LOCUS       DATCBJ010000001         2773 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_35933, whole genome shotgun sequence.
ACCESSION   DATCBJ010000001 DATCBJ010000000
VERSION     DATCBJ010000001.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2773)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2773)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2773
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_35933"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..520
                     /locus_tag="VII50_00005"
     CDS             <1..520
                     /locus_tag="VII50_00005"
                     /inference="COORDINATES: protein motif:HMM:NF024491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HEY5049252.1"
                     /translation="DVAAIEQVFNADFTHARITASPATDLVWSPNQSEPALLSLIDSA
                     TSSLLIENEEMADKPVTAALVSAAHRGVRVVVVMTDQTDWHSAFASLVTAHAVVRIYA
                     ATASLYIHAKVVVADAGTPHQRAFVGSENFSNASLNQNRELGVETTDPGVVAGLTTTL
                     NSDAAGAQSWRS"
     gene            611..985
                     /locus_tag="VII50_00010"
     CDS             611..985
                     /locus_tag="VII50_00010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5049253.1"
                     /translation="MDENRALRVVDALRARGIDARMSKEGVYQFGVRIALSDAREAVW
                     DSDDTAELEAQVMRDGMLVGFVPVIEGSADFDEAQIIDAIANTDYDQPIAHRAAAPAP
                     AAPALAPEGGVFRRFLGGFRSD"
     gene            complement(1006..1329)
                     /locus_tag="VII50_00015"
     CDS             complement(1006..1329)
                     /locus_tag="VII50_00015"
                     /inference="COORDINATES: protein motif:HMM:NF020317.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GYD domain-containing protein"
                     /protein_id="HEY5049254.1"
                     /translation="MAKYLFKASYTPEGIKGVMKEGGSSRTNAVEKTVAGLGGTLESL
                     YFAFGATDVLVTADLPDHASAVAFAAAVGSTGAFSTVETVVLLTPGDIDAAVHKSVDY
                     RAPGS"
     gene            1494..>2773
                     /locus_tag="VII50_00020"
     CDS             1494..>2773
                     /locus_tag="VII50_00020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0098561 - methyl accepting chemotaxis
                     protein complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HEY5049255.1"
                     /translation="MEKEQGQVEESPVPDGVRWAHYCAAFLVTAYFVSVAVRRVGSHD
                     APIDGWGVDLFEVSVGVLCMLRYRHGSWRTARSTVTFFPLILGSACILWGFGDTARTI
                     ESLGGATPPTPSAADGFGLMFFPLCYVALVLLLRGESKGFAITPWLDRAIGALGFASV
                     AAAFVLQPVLKAVGGWSLATATGLAYPIGDLLLLGVVAGGFTTASRARQSVLVVVGVA
                     QVVNFAGDMYVLVQPDSTIGNLANALAWPISILMLSAAAWLQPANARSTSKVRAGGFM
                     LPGLGAVASLTILVFASVGSVGKDAVGLATATLLVAGIRMTLTVRKAQGMNDERQYAM
                     TERQAVLLKVLNEVARNAEMLTAASERLTATATKLSSGADQASSQADVVANASRLIGA
                     STQSVATGTEQMTASIAEIARNAADAAMAGAEAMR"
BASE COUNT          444 a          911 c          923 g          495 t
ORIGIN      
        1 cgacgtggct gccatcgagc aggtgttcaa cgccgacttc acgcacgcgc gcatcaccgc
       61 gtcgccggcc accgatctgg tgtggagccc gaatcagtcc gagcccgcgc tgctgtcgct
      121 gatcgactcg gcgacgtcga gcctgctgat cgagaacgag gagatggccg acaagccggt
      181 caccgccgca ctggtcagtg cggcgcaccg cggcgtccgg gtcgttgtcg tcatgaccga
      241 ccagaccgat tggcactccg cattcgcctc gctcgtcacc gcgcatgcgg tcgttcgcat
      301 ctacgcagcc actgcgtcgc tctacattca cgcgaaggtc gtcgtggcgg acgccggaac
      361 ccctcaccag cgcgcgttcg tcggatccga gaacttctcc aacgcatcgc tgaaccagaa
      421 ccgcgaactc ggtgtcgaga ccaccgaccc cggggtcgtg gcgggcctga ccacgaccct
      481 caactccgac gcagcgggcg cgcaaagctg gcgctcctag ctcgcaaccc tggccccggg
      541 tgaggctccc gacatcgccg tccggcgttc ggtagttttg aggcctgacg agcagcggga
      601 gttttgaggc atggacgaga accgggcact acgagtcgtc gacgcgctgc gggcgcgcgg
      661 catcgacgcg cgcatgtcga aagagggcgt ttaccagttc ggcgtgcgga tcgcgctctc
      721 cgatgcccgc gaggcggtgt gggattccga cgacaccgcc gagctggagg ctcaggtcat
      781 gcgcgacggc atgctcgtcg ggttcgtccc ggtgatcgaa gggtccgccg acttcgacga
      841 agcgcagatc atcgacgcca tcgcgaacac cgactacgac cagccgatcg cccaccgcgc
      901 ggcggcaccg gcccctgccg ctccggcgct cgcacccgaa ggcggcgtgt tccgccggtt
      961 cctcggcggc tttcgcagcg actgagtgcc tacctggcgc aggccctagg agcctggcgc
     1021 ccggtagtcg accgacttgt gcacggcggc gtcgatatcc ccgggggtca gcaggacgac
     1081 cgtctcgacc gtgctgaacg ctccggtgga tccgaccgcg gccgcaaaag cgacggcgga
     1141 ggcgtggtca gggaggtcgg ccgtgactag aacgtccgtg gcgccgaacg cgaagtagag
     1201 cgactccagc gttccgccca agcctgcgac ggtcttctcg acggcattcg tccgggagct
     1261 gccgccctct ttcatcacgc ctttgatgcc ttctggggtg tatgacgcct tgaacaggta
     1321 cttggccacg gtggccacct ctctttcgtc cggctcatct aatgggtccc cgtttgggcg
     1381 atcaatggtg gcggagccta aaggctgccc aaggacgtcc gatacgaacg tgacgaatcg
     1441 tcgaatcggg tcgcgcgcgt ggcgatccgt acccagatgg gccgcgtgca ccgatggaga
     1501 aagaacaagg ccaggtcgag gagagccccg tccctgacgg cgttcgatgg gcccactact
     1561 gcgccgcgtt cctcgtgacg gcctatttcg tttccgtcgc agtccggcgg gtcggctcgc
     1621 atgacgcccc catcgacggc tggggagttg acctattcga agtctccgtt ggcgtcctgt
     1681 gcatgcttcg ctaccggcac gggtcatggc ggacggcgcg atccacggtc acgtttttcc
     1741 cgttgatatt gggatctgcc tgcatcctgt ggggcttcgg cgataccgcg cggaccatcg
     1801 aatcgctcgg tggagcgacg ccgccaactc cgtcggccgc cgacggattc ggcctgatgt
     1861 tcttcccgtt gtgttacgtc gccctggtgt tgctgctgcg tggcgagtcc aaaggattcg
     1921 cgatcactcc ttggcttgac cgggcgatcg gagcgctcgg tttcgcctct gtcgctgccg
     1981 cattcgtttt gcagcccgtc cttaaagcag tcggtggttg gtccttggcg acagcgactg
     2041 gcctggcgta tccgatcggt gacctgctgc ttcttggggt cgtggcgggc ggcttcacca
     2101 cggcctccag agcgcgccag agcgttctcg tcgttgtcgg cgtggcgcag gtggtcaact
     2161 tcgccggcga catgtacgtg ctggtgcagc cggatagcac gatcggcaac ctggcgaacg
     2221 cgctcgcgtg gccgatctcg atcttgatgc tgtcggcggc ggcctggctg cagccggcca
     2281 acgcacggag cacgagcaaa gttcgagccg gtggcttcat gctgcccggt ctcggcgcgg
     2341 tcgcgagcct caccattttg gttttcgcca gcgtcggaag cgtcggcaag gatgccgtcg
     2401 ggctagcgac ggcgacactt ctcgttgccg gcatccggat gactttgacg gtgcgtaagg
     2461 cacaggggat gaacgacgag cgtcagtacg cgatgaccga acgccaggcg gtgctcctga
     2521 aggtactcaa cgaggtcgct cgcaacgcgg agatgctaac cgcggcctcc gagcggctta
     2581 ccgcgactgc caccaagctg agcagcggag ccgaccaggc ctcctcacag gcagatgtcg
     2641 tcgccaatgc aagcaggctc atcggagcga gcacccagtc ggtcgcgacc ggcaccgagc
     2701 aaatgacggc gtcaatcgcg gagatcgccc gaaacgcggc ggacgcggcg atggcgggcg
     2761 ctgaggcgat gcg
//
LOCUS       DATCBJ010000002         6663 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_551426, whole genome shotgun sequence.
ACCESSION   DATCBJ010000002 DATCBJ010000000
VERSION     DATCBJ010000002.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 6663)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6663)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6663
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_551426"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..243
                     /locus_tag="VII50_00025"
     CDS             <1..243
                     /locus_tag="VII50_00025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrB/UvrC motif-containing protein"
                     /protein_id="HEY5049256.1"
                     /translation="GRQMSRGKSPVPGLSSRGAGAHARELAGMPREELGELIAQLTEQ
                     MHTAATELHFELAARLRDEIHELKKELRGMEAAGVR"
     gene            363..599
                     /locus_tag="VII50_00030"
     CDS             363..599
                     /locus_tag="VII50_00030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5049257.1"
                     /translation="MSADRGRTSAKSHAVRYLFVALFAAAATKASSHPAPRKVAKKAI
                     DLGRAWSNEQLNRALQAPVIKSQGERWRAMHPDA"
     gene            617..883
                     /locus_tag="VII50_00035"
     CDS             617..883
                     /locus_tag="VII50_00035"
                     /inference="COORDINATES: protein motif:HMM:NF015735.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HEY5049258.1"
                     /translation="MLIYESPEDLDMDELRARFPAHRARWSIFQEQGTLLLIGPYANR
                     EGAQAVFTTREAAEEFAATDPFALNGLVAKWHMQAWNEALLEPL"
     gene            1237..1461
                     /locus_tag="VII50_00040"
     CDS             1237..1461
                     /locus_tag="VII50_00040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5049259.1"
                     /translation="MPKRRTAQWYRLGGLIWALVAALYWIAEIVQPRPLHLAFGILFT
                     CVAVIYFVGAKELARRSAARLASRWELPDK"
     gene            complement(1489..2154)
                     /locus_tag="VII50_00045"
     CDS             complement(1489..2154)
                     /locus_tag="VII50_00045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEY5049260.1"
                     /translation="MASDEYTGDVKVGGPVAVRELSGLRVTKVAVGPYDNNAYLLRCT
                     ATGDTMLIDAAAEPDRLLAARADGLLVSVVTTHQHPDHWQGLEAVVGVTGPAVIAHPL
                     DAPGLPIPVTETVEDGDVLAIGDVQVKVIHLKGHTPGSIAIVYGWESAAPHIFTGDSL
                     FPGGPGNTRKNVADFTSLMDDLQAKVFGVFPDATWVYPGHGGDTTLGVERPHLQDWRA
                     RGW"
     gene            complement(2193..2909)
                     /locus_tag="VII50_00050"
     CDS             complement(2193..2909)
                     /locus_tag="VII50_00050"
                     /inference="COORDINATES: protein motif:HMM:TIGR03083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maleylpyruvate isomerase family
                     mycothiol-dependent enzyme"
                     /protein_id="HEY5049261.1"
                     /translation="MSQHWDPAPDSAEIEAATSRLLATAATLSDADVLAPSGLPEWSR
                     GHVLTHIARNADSLVNLLTSAATAVPHPQYASKEARDTAIDAGAARPAAEQVADIEAS
                     HRRFEDAVSAMPPGGWQHDVRWLSGETRPATKILDARLREVAIHHIDLDGAYTASDWS
                     SQFALRILVSVLPAFEVRGIEPVTLVPSDVDVRIDLAGGSAVEVRGPASALAIWVLGR
                     DDGSTLEVTGGQLPTPPAWT"
     gene            3092..5977
                     /gene="uvrA"
                     /locus_tag="VII50_00055"
     CDS             3092..5977
                     /gene="uvrA"
                     /locus_tag="VII50_00055"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HEY5049262.1"
                     /translation="MIRGAREHNLKDVSLDLPRDALIVFTGLSGSGKSSLAFDTIFAE
                     GQRRYVESLSAYARQFLGQMDKPDVDFIEGLSPAVSIDQKSTSRNPRSTVGTITEVYD
                     YLRLLYARIGHPHCPVCGRPISRQTPQQIVDRILELDEGSRFQVLAPLIRGRKGEYAD
                     LFGDLQTKGFARVRVDGVVYPLTEPPTLKKQEKHTIEVVVDRLAVKESAKRRLTDSVE
                     TALKLAGGLLTLEFVDLPDDDPHRERVFSEHLACTYDDLSFEELEPRSFSFNSPFGAC
                     TECTGLGTKLEVDPDLIIADGSLSIAKGVIAPWAGGHGSDYFARLLDSLADTMGFSTS
                     APWNKLPIKVRRAVLYGYDEQVHVRYKNRYNRERSYYTTYEGVIPFIERRHGEVESDW
                     SREKFEGYMREVPCAECHGKRLKPVSLAVTLDDKSIADVAGLPIGECATFLRGLELSP
                     REKQIAERLLKEVNSRLGFLLDVGLHYLSLDRAAGTLAGGEAQRIRLATQIGSGLVGV
                     LYVLDEPSIGLHQRDNHRLIDTLIRLRDLGNTLIVVEHDEDTIRTADWVVDIGPGAGE
                     HGGQVVVSGSVKDLLASKDSLTGAYLSGRRKIDVPAKRRAPDPKRALVVKGAREHNLR
                     DVDVSFPLGCLVAVTGVSGSGKSTLVNDILYAALARELYGAKTVPGRHVRITGLDQVD
                     KAIHVDQSPIGRTPRSNPATYTGVFDNIRKLFAETTEAKIRGYLQGRFSFNVKGGRCE
                     ACAGDGTIKIEMNFLPDVYVPCEVCHGARYNRETLDVHFKGKTIADVLDMPIEEAAEF
                     FAAIPAIARHLVTLVEVGLGYVRLGQPAPTLSGGEAQRVKLASELQKRSTGRTVYVLD
                     EPTTGLHFEDVRKLLGVLQSLVDKGNTVIVIEHNLDVVKTADWVIDMGPEGGSGGGLV
                     VAEGTPESLAGNEASHTGVFLRHLLAAQPATAPPAARRPRKMAAARA"
     gene            6042..>6663
                     /locus_tag="VII50_00060"
     CDS             6042..>6663
                     /locus_tag="VII50_00060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HEY5049263.1"
                     /translation="MVDPASYRPATGSIPESPGVYRFRDSQDRVVYVGKAKNLRSRLN
                     SYFADVGSLHPRTQSMVCSASRVDWTVVATEVEALQLEYNWIKEFDPRFNVKYRDDKS
                     YPYLAVTLADDVPRSMVMRGAKRRGVRYFGPYSHAWAIRETLDLLLRVFPMRTCSNGV
                     YRRAEQTGRPCLLGHIGKCAAPCVGWVSIDEHRKIVEDFCDFMAGHP"
BASE COUNT         1127 a         2170 c         2202 g         1164 t
ORIGIN      
        1 ggccggcaga tgagccgggg caagtccccg gtgcccgggc tgtcgtctcg aggtgccgga
       61 gcgcacgccc gtgagctcgc cgggatgccg cgcgaggagc tgggcgagct catcgcccag
      121 ctcaccgagc agatgcatac cgccgcgaca gagctgcact ttgagctggc ggcgcgactg
      181 cgcgacgaga tccatgagct caagaaggaa ctgcgcggaa tggaggccgc tggcgtccgc
      241 tgatcgccga tcgcccggcc gggtggccgc cacaacaaat cctgttggaa tcggccgtcc
      301 gccgatatcg tcggctcgct gggtgcgtgt gcgtcgctga gtgtcctcgt taggagcatc
      361 ggatgagtgc cgatcgcggt cggacgagcg ctaagagtca cgcggtgcgg tatctgtttg
      421 tcgcgttgtt tgctgctgcg gcgaccaaag cgagcagcca ccccgccccg cggaaagttg
      481 ccaagaaagc gatcgacctc ggccgggcgt ggtcgaacga gcaactgaat cgagccctgc
      541 aagcgccggt gatcaagtcg cagggtgagc gctggcgcgc gatgcacccg gacgcctagg
      601 cccgattgtg aagtaggtgc ttatctacga gtcgccagaa gacctcgaca tggacgaact
      661 gcgcgcgcgg ttccctgcgc accgcgctcg gtggtcgata tttcaggagc agggcacact
      721 cctgttgatc ggcccgtacg cgaaccgcga gggcgcccag gcggtgttta cgacgcgcga
      781 agccgccgag gaatttgccg caactgatcc gttcgcgctg aacggtcttg tcgccaagtg
      841 gcacatgcag gcgtggaacg aggcgttgct agagccgctg tgatcggcgg tacttacaga
      901 cgcgggtact tgcttctggt cggtggcgcg gtaggacgga cgccgggccg tcgacgccgg
      961 acatgggttc tgctgctcct ggcggcggca gacatattcg cagcacgccg gatggtgtgg
     1021 cctttgcgcc gcgctcggcg atccatcaag cccagcagcg tgccgactgc gagcacaacg
     1081 gcagcgatga tgagccagac gtacacgacc attgctacct ccagcctctg tgccgaagtt
     1141 gcacggccta gccacaggaa cctgatacag atgtgccacg ccacacccca cgtgttgccg
     1201 tagtgggacg gcgcgggggg cttgtaatct gtggcgatgc cgaaacgacg aacggcccag
     1261 tggtaccggc tgggtggcct gatctgggcg ctggtagcgg cgttgtactg gatagccgag
     1321 atcgttcagc cgcgacccct gcaccttgct ttcgggatcc tgttcacctg tgtcgccgtc
     1381 atctacttcg ttggcgccaa ggagttagcc cgtcgttccg cggcgcggct agccagccgt
     1441 tgggaactgc cggacaagta gccgaacttc cgcgcacagt tggctgacct accagccgcg
     1501 cgcgcgccag tcctgtagat gtgggcgctc gacacctagc gtcgtgtcgc cgccatggcc
     1561 gggatacacc catgtggcgt cagggaagac gccgaagacc ttcgcctgga ggtcgtccat
     1621 caatgaggtg aagtcggcga cgttcttgcg ggtattccca ggcccgccag gaaataacga
     1681 gtcacccgtg aagatgtgcg gtgcagccga ctcccagccg tacacaatcg cgatgcttcc
     1741 tggcgtgtgg cctttcaagt gaatgacctt cacttgcacg tcgccgatcg ccagcacgtc
     1801 gccgtcctcg accgtttctg tcaccggtat cgggaggccg ggcgcgtcga gcggatgagc
     1861 gataaccgca gggcctgtga cgccgacgac tgcctcgaga ccttgccagt gatccggatg
     1921 ctgatgtgtc gtcacgacac tgaccagcaa cccgtcggcg cgcgcggcca agaggcggtc
     1981 gggttcggct gctgcatcga tcaacatcgt gtcgcccgtc gcggtgcacc ggagcaggta
     2041 ggcgttgttg tcatagggcc cgaccgcgac cttggtgacc cgcaatccgg acagctcgcg
     2101 cacagcgacc gggccgccga ctttcacatc gccggtgtat tcgtcagatg ccatgccgat
     2161 cattgtgtcc cgtccgcggt gaccggacga gatcaggtcc aggccggcgg cgtcggcagc
     2221 tgcccgcccg tcacctcgag tgtcgagccg tcgtcgcgac cgagcaccca gatggccagc
     2281 gccgacgcgg ggccgcggac ctcgactgcc gagccgccgg cgaggtcgat tcgcacatcg
     2341 acgtcgctcg gcaccagcgt gaccggctcg atcccgcgga cctcgaacgc gggcaacacc
     2401 gagaccaaga tccgcaacgc aaactgcgac gaccagtcgg acgccgtgta ggcgccgtcg
     2461 aggtcgatgt gatggatcgc cacctcgcga agccgcgcgt cgaggatctt cgtcgctggc
     2521 cgtgtctcgc ccgacagcca gcgcacgtcg tgctgccagc cacctggcgg catcgccgat
     2581 acagcgtcct cgaatcgccg gtgcgacgcc tcgatatcgg ccacctgctc ggccgccggg
     2641 cgagcagccc cggcgtcgat ggcggtatcg cgcgcctcct tgctggcgta ctggggatgc
     2701 gggacggcgg tcgccgccga ggtcagcagg ttgaccaggc tgtcggcgtt gcgggcgatg
     2761 tgggtcagca catgaccgcg ggaccactcg ggcaggcctg agggcgccag cacatcggcg
     2821 tccgacagcg tcgcggcggt ggcgagcaac cgggacgttg ccgcctcgat ctcggcggaa
     2881 tcgggcgcgg ggtcccagtg ctggctcacc ttccggacgc tacctgcgaa ccggtgtcaa
     2941 ccgctcccat tttgtgcgcc ggagtttctg tcggaccccg cacctacgat ggacgagccc
     3001 ggggtgcatc cgggcgagcg ggctcgagcc ggcaggcccg agaccagccg atgtgagggg
     3061 atggggcagc cgcgtcatgg tcgatcgtct ggtgatccgc ggcgctcgcg agcacaacct
     3121 caaggacgtg tcgctcgatt tgccgcgcga tgcgttgatc gtcttcaccg ggctgtccgg
     3181 ctcagggaag tcgagcctcg ccttcgacac gatcttcgcc gagggccagc ggcgctacgt
     3241 cgagtcgctg tcggcctacg cccggcagtt cctgggccag atggacaagc ccgacgtcga
     3301 cttcatcgag ggtctctcgc ccgcggtctc gatcgatcag aaatcgacct cgcgcaaccc
     3361 gcgatcgacg gtcggcacga tcaccgaggt gtatgactac ctgcggctgc tctacgcgcg
     3421 gatcggtcat ccgcactgcc cggtctgcgg tcggccgatc tcacggcaga ccccgcaaca
     3481 gatcgtcgac cgaattctcg agctcgacga gggcagccgg ttccaggtgc tcgcgccgct
     3541 gattcgcggt cgcaaggggg agtacgccga cctgttcggc gacctgcaga ctaagggatt
     3601 cgcgcgagtg cgcgtcgacg gcgtggtgta tccgttgacc gagccgccga cgctgaagaa
     3661 gcaggagaag cacaccatcg aggtcgtcgt cgaccggctc gcggtgaaag agtccgcgaa
     3721 gcgccggttg acagactcgg tcgagaccgc gctgaagctc gccggcggtc tgcttaccct
     3781 ggagttcgtc gatttgcccg acgacgatcc gcatcgcgag cgcgtcttct ccgagcacct
     3841 cgcgtgcacc tatgacgact tgtcgttcga ggagctcgag ccacgctcgt tctcgttcaa
     3901 ctcgcccttt ggtgcatgca cggaatgcac cggtctcggc accaagctcg aggtcgatcc
     3961 cgacctcatc atcgccgacg ggagcctgtc catcgcgaag ggcgtgatcg cgccctgggc
     4021 gggcggccac ggctccgatt acttcgcgcg actcctcgat tcgctcgccg acacgatggg
     4081 gttcagcacc tcggcgccgt ggaacaagct gcccatcaag gtgcgcaggg ccgtgctgta
     4141 cggctacgac gagcaggtcc acgtccgcta caagaaccgc tacaaccgcg agcgttcgta
     4201 ctacaccacc tacgaaggcg tgatcccatt catcgagcgc cgccacggcg aggtcgagag
     4261 cgattggagt cgcgagaagt tcgagggtta catgcgcgag gtgccgtgcg ctgagtgcca
     4321 cggcaagcgg ttgaagccgg tgtcgctcgc ggtgacgctc gacgacaaat ccattgcgga
     4381 cgtcgcgggg ttgcccattg gcgaatgcgc gacgttcctt cgtggtctcg aactgtcgcc
     4441 gcgcgagaag cagattgccg agcggctgct caaagaagtc aactcccgct tgggatttct
     4501 gctcgatgtc ggcctgcatt acctgtcgct cgatcgggcc gccggtacgc tcgccggcgg
     4561 tgaagcccag cgcatccggc tggccaccca gatcgggtcg gggctggtcg gcgtcctgta
     4621 tgtgctcgac gagccgagca tcggtctgca tcagcgcgat aatcaccggc tgatcgacac
     4681 gttgattcgg ctgcgcgatc tcggcaacac cctcatcgtg gtcgaacatg atgaagacac
     4741 cattcgcacc gcagactggg tcgtcgacat cggaccaggt gcgggcgagc acggcggcca
     4801 ggttgtcgtg tcgggttcgg tgaaggatct gcttgccagc aaggactcct tgaccggcgc
     4861 ctacttgtca ggacgccgca agatcgacgt gccggcgaaa cgtcgcgcac ccgatccgaa
     4921 gcgcgcacta gtagtcaagg gcgcccgcga acacaatctg cgcgatgtcg acgtgagctt
     4981 cccgctcgga tgtctcgtcg cggttaccgg cgtctccggc tccggcaagt cgacgttggt
     5041 caacgacatc ctctacgcgg ccctcgctcg cgagctctat ggcgcgaaga cggttccggg
     5101 tcgccacgtg cggatcaccg gtctcgacca ggtcgacaag gcgatccatg tcgaccagag
     5161 cccgatcggc cgcacaccac gatcgaaccc ggccacctac accggagtct tcgacaacat
     5221 ccgcaagctt ttcgccgaga ccaccgaagc gaagatccgc ggctacctcc aaggccgctt
     5281 ctcgttcaac gtgaagggcg gtcgctgcga ggcgtgcgct ggcgacggca ccatcaagat
     5341 cgagatgaat ttcctgcccg acgtctacgt cccgtgcgag gtgtgtcacg gcgcgcgcta
     5401 caaccgcgag acgctcgatg tgcatttcaa gggcaagacc atcgccgacg tgctcgacat
     5461 gccgatcgag gaagccgccg agttcttcgc cgccatcccg gcgatcgcgc ggcatctggt
     5521 cacgctggtc gaggtcggtc tcggctacgt gcggctcggg cagccagcgc cgacgctgtc
     5581 tggcggcgag gcgcagcggg tcaagctcgc ctccgaactg cagaagcggt cgaccggccg
     5641 cacggtctac gttctcgatg aaccgacgac ggggctgcat ttcgaagatg tccgcaagct
     5701 gctcggcgtc ctgcagtctc ttgtcgacaa gggcaacacc gtcatcgtga tcgagcacaa
     5761 cctcgacgtg gtgaagaccg cggactgggt catcgacatg ggtcccgagg gtggctccgg
     5821 cggcgggctc gtggtcgccg aaggcactcc ggagtccctc gctggcaacg aagcaagcca
     5881 caccggggtg ttcttgcggc acctattggc cgcccaaccg gcgacggccc cgccggcagc
     5941 tcgacgtccc cggaagatgg ctgcggcacg ggcctagtcg ccgtccggcg gcaggtcaca
     6001 gcgcgccgcg ttctgtcgcc gccgtcggta ggctggacgc tgtggtagac cccgcgagtt
     6061 atcggcccgc gacgggctcg attcccgaat cgccaggcgt ctatcggttc cgcgattcgc
     6121 aggatcgggt cgtctacgtc ggcaaggcga agaaccttcg ttcccggctc aactcgtact
     6181 tcgccgacgt cgggtcgctg catccccgta cgcagtcgat ggtctgcagt gcgagtcgtg
     6241 tcgattggac cgttgtcgcg acagaggtcg aggcgctcca gctcgaatac aactggatca
     6301 aggagttcga cccgcggttc aacgtgaagt accgcgacga caagagctac ccttacctcg
     6361 ctgtgacgct cgccgacgac gtcccacgtt ccatggtgat gcgcggtgcg aaacgcaggg
     6421 gcgtgcgcta tttcggtccg tattcgcatg cctgggcgat ccgagaaact ctcgatctgc
     6481 tgcttcgcgt ctttcccatg cgtacctgtt cgaatggggt ctaccggcgg gctgagcaga
     6541 ccggccggcc ttgcttgttg ggtcacatcg gcaagtgcgc agcgccgtgc gtcggctggg
     6601 tctcgatcga cgagcaccgc aagatcgtcg aggacttctg cgacttcatg gcgggccatc
     6661 ccg
//
LOCUS       DATCBJ010000003         2986 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_515835, whole genome shotgun sequence.
ACCESSION   DATCBJ010000003 DATCBJ010000000
VERSION     DATCBJ010000003.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2986)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2986)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2986
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_515835"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..228
                     /locus_tag="VII50_00065"
     CDS             <1..228
                     /locus_tag="VII50_00065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004561228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HEY5049264.1"
                     /translation="IGFITSGPLVAAAVEGPRAIETWRAMMGPTDPVKAPSGTIRGDF
                     GLIVTENLVHGSDSPASAAREVALFFPDLLT"
     gene            533..1558
                     /locus_tag="VII50_00070"
     CDS             533..1558
                     /locus_tag="VII50_00070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019983803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HEY5049265.1"
                     /translation="MSFLGRDMAVDLGTANTLVYVRGRGIVLNEPSVVAINTNTGGIL
                     AVGIEAKRMIGRTPGNIVAIRPLKDGVIADFDTTERMLRYFIQKVHRRRYLARGPRVV
                     VCVPSGITGVEQRAVKDAGYAAGARRVYIIEEPMAAAIGAGLPVHEPTGNMVVDIGGG
                     TTEVAVISLGGIVTSQSIRIGGDELDSSIINYVKKEYSLMLGERTAEEIKMAIGSAFP
                     APDEPHAEIRGRDLVSGLPKTIVVSAAEIRKAIDEPVNAIVDAVKTTLDKCPPELAGD
                     IMDRGIVLTGGGALLKGLDERLRHETGMPIHITERPLDSVAMGSGKCIEEFEALQQVL
                     ISEPRGR"
     gene            1623..2723
                     /gene="mreC"
                     /locus_tag="VII50_00075"
     CDS             1623..2723
                     /gene="mreC"
                     /locus_tag="VII50_00075"
                     /inference="COORDINATES: protein motif:HMM:NF016011.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005618 - cell wall [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HEY5049266.1"
                     /translation="MRDSRRTRVVLALLLLTAFTLITLDYRSRGGGFFGSIRRTASSV
                     FGPVERVAADVVRPVRHAVQAVGDVGSNNKQVTDLQKEVATLQQQLRLQPFDTARETE
                     LEQLLHVSAVGQYKLLYAQVIAVSATDGFEWTATIDAGSSDGLKANMTVLNGDGLVGR
                     LKIVGSTTSTVLLAIDPEFNVGVRLPNNAMGVVSGHGRSAMSMEMLDPTARVKVGDGL
                     VTQGSLDQSPFVWGVPVGSVISATNPLAGDVQTGEVKPYVDFSTLDLVGVVVEPPRVD
                     PRDALLAPPLPTITVTTTATATATVTATPLASFTPTFSFSATPTGKPSATPTSKVTPK
                     PTVTPSSTPTPRPTSTPSGSPSATPTASATHG"
     gene            2716..>2986
                     /gene="mreD"
                     /locus_tag="VII50_00080"
     CDS             2716..>2986
                     /gene="mreD"
                     /locus_tag="VII50_00080"
                     /inference="COORDINATES: protein motif:HMM:TIGR03426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HEY5049267.1"
                     /translation="MAEVPRAALIVVLVLLACVIDAVLLDPLNLPGSPPNLLVLVVAA
                     IALVLGPVGGAVTGFLAGLAADVLPPADHLIGRYALVLCLAGYLVG"
BASE COUNT          494 a          988 c          974 g          530 t
ORIGIN      
        1 atcggcttca ttacctccgg accgctggtc gctgccgccg tcgaaggccc gcgggccatc
       61 gaaacctggc gggccatgat ggggccgacg gacccggtga aagcaccatc aggaacgatc
      121 aggggcgatt tcggactcat cgtcaccgag aatttggtgc acgggtcgga ctcgccagcg
      181 tcggcagccc gagaggtcgc cctcttcttc cctgacttgc tcacgtaggc cggcccccgc
      241 cgcctcgcct gcgcgttttt gaaagtgatc acgtaaggca gccgtcaggg tcctcaggta
      301 agtgtgggca cctgcctcag gacctcccgg gctgcttaac gtggtcaacc gccggcggag
      361 ctgggagcgg gctgaggggc gtcggccggt tttggccgag gtggtgggtc accgcgtagc
      421 atggccaggc tgtccccgcc ccggggatcc cgggcactgc agcaatcgtt tcgcagcagt
      481 cgagttgtcg cgtcagtcga tcggaaggtt tgcagcaccc atggcctcca acatgtcgtt
      541 cctcggccgg gacatggccg tcgacctcgg caccgccaac acgctggtct acgtccgtgg
      601 ccgtggcatc gtcttgaacg agccctccgt ggtggccatt aacaccaata ccggcggcat
      661 tttggcggtc ggcatagagg ccaagcggat gatcggtcgg acgccgggca acatcgtcgc
      721 gatccgcccg ctgaaggacg gcgtcatcgc cgacttcgac acgacggaac gcatgctccg
      781 ttacttcatc cagaaggtgc accgccgccg ttacctcgcg cgcggcccac gcgtcgtcgt
      841 gtgtgtgccg agtggtatca ccggcgtcga gcaacgcgca gtgaaggacg ccggctatgc
      901 ggcgggcgca cgacgcgtct acatcatcga ggagccgatg gcggcggcga tcggtgccgg
      961 cctgccggtt cacgagccca ccggcaacat ggtcgtcgac atcggcggtg gcaccaccga
     1021 ggtcgcggtg atctcactcg gtggcatcgt caccagccag tccatccgca tcggcggcga
     1081 cgagctcgac agttcgatca tcaactatgt gaagaaggaa tactcgctga tgctcggcga
     1141 gcgaaccgcc gaggaaatca agatggcgat cggctcggcg ttccccgcgc ctgacgaacc
     1201 gcatgcggag attcgcggtc gcgacctcgt tagtgggttg ccgaagacga tcgtggttag
     1261 cgccgccgag attcgtaagg ccatcgacga accggtcaac gccatcgttg atgcagtgaa
     1321 gacaaccctt gacaagtgtc cgccggaact tgccggcgac atcatggacc gcggcatcgt
     1381 gctcaccggt ggtggcgcgt tgctaaaggg cctcgacgag cggctgcggc acgagacggg
     1441 aatgccgatc cacatcaccg agcggccgct cgactcggtc gcgatgggtt cgggtaagtg
     1501 catcgaggag ttcgaggccc tgcagcaggt tctcatctcc gagccgcgcg gtcgctgacc
     1561 gccgacccgg tcctggtgtc gcgcaacacg tctgaggtct tcatgaatcg ccgcgtgcgc
     1621 ttgtgaggga tagtcgccgc acccgcgtcg tcctcgcgtt gttgctgctg acggcattca
     1681 cgttgatcac ccttgactac cgatcgcgcg gcggcggatt ctttggttcc atccgccgta
     1741 ccgcgtcctc ggttttcggg ccagtcgaac gggttgccgc cgatgtggtg cggccggtcc
     1801 ggcacgccgt acaagccgtg ggcgacgttg gtagcaataa caagcaggtc accgatctac
     1861 aaaaggaagt tgccaccctt caacaacagc tgcgcctgca gccgttcgac acagcacggg
     1921 agaccgagct cgagcaactc ctgcacgtgt cggccgtcgg ccagtacaag ctgctctacg
     1981 cgcaggtgat cgcggtcagt gcaacggacg gcttcgagtg gacggcgaca atcgacgccg
     2041 gctcgagtga cggtctcaag gcgaacatga ccgtcctcaa cggcgacggt ctcgtcggcc
     2101 ggttgaagat cgtcggttcg acaacatcga cggtgctgct tgcgatcgat ccggagttca
     2161 acgtcggtgt tcggctgccc aacaacgcca tgggagtcgt atcggggcac ggtcgctcgg
     2221 cgatgagcat ggagatgctc gacccgacgg ctcgggtgaa ggtgggcgac ggactggtga
     2281 cacagggatc cctcgaccag agtccgttcg tgtggggcgt gccggtcggc agcgtgatct
     2341 cggcgaccaa tccgctcgca ggcgacgtgc agaccggcga ggttaagccc tacgtcgact
     2401 tcagcacgct cgacctcgtc ggcgtcgttg tcgagccgcc acgggtcgac ccgcgcgacg
     2461 ccctccttgc gccgccattg ccgaccatca ccgtgacaac cacggcgacg gcgacggcga
     2521 ccgtcacggc gactccgctc gcgtcgttca caccgacctt cagcttcagc gcgacgccga
     2581 ccggaaagcc gagcgcgacg cctacgtcaa aggtgacccc gaagccgacc gtgacgccaa
     2641 gcagcacgcc gacgccgcga cccacttcga cgccgtccgg ttccccgagt gctaccccga
     2701 ctgcgtcggc tacccatggc tgaggttccg cgggcggcgc tgatcgtggt cctcgtcctg
     2761 ctcgcctgcg tgatcgacgc ggtgctcctc gacccgctca atctgccggg ttcaccgccc
     2821 aacctgctgg tgctcgttgt cgcggcgatc gcgctggttc tcggcccggt cggcggagcg
     2881 gtgaccggct tcctcgccgg tctcgccgcc gacgtgctgc cgccggctga tcatttgatc
     2941 ggccgctatg cgctggtcct ctgcctggct gggtacctcg tcggcc
//
LOCUS       DATCBJ010000004         2090 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_441803, whole genome shotgun sequence.
ACCESSION   DATCBJ010000004 DATCBJ010000000
VERSION     DATCBJ010000004.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2090)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2090)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2090
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_441803"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            239..586
                     /locus_tag="VII50_00085"
     CDS             239..586
                     /locus_tag="VII50_00085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5049268.1"
                     /translation="MTSLADLLVDATRLLVVEGIEYDPSSRRKTRALFRDTDPASIAQ
                     LVASLATGPSPDDVALMQWPSMTFVLFEGDHELARVEYLGGWLRWSGWQGDRPLIRPA
                     DLASWLTAHGLTA"
     gene            865..>2090
                     /locus_tag="VII50_00090"
     CDS             865..>2090
                     /locus_tag="VII50_00090"
                     /inference="COORDINATES: protein motif:HMM:NF033542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HEY5049269.1"
                     /translation="MGAVVIGMDPHKRTATIEVMDGDETILGGGRYATDVAGYQAMLA
                     FGQQFADRTWAIEGCNGIGKHIADRLLADGEQVVDVPPKLSARTRVFATGQGRKTDAT
                     DAHSVALVGTRMSGLRPVVADEELAVLRLLADRRRSLGQDHTRMVAQLHRILLELIPG
                     GAKKQLSAAQAKVLLGKVRPRDTAGKTRKRVCAELVADLQRIHERTKAADKELKALLV
                     ATGTTLMTLHGIGPSGAARLLVEVGDITRFPTRAHFASWTGTAPIDASSGDHVRHRLS
                     RGGNRQINRTLHIMAVAQLRNPTKGRAYYNRKKVDGKAPMEAMRCLKRRLSDVVYRQL
                     LADAIRPSKAGPGGHSGAALDSSAVDSNPDIDASKKSLPGPANTHVTRAKSPRPDRSS
                     TRSRRPATAPNRSRRQA"
BASE COUNT          388 a          664 c          675 g          363 t
ORIGIN      
        1 ctcgatgtag gggtacccag cgtcaatgtg cctgtcgaag ccgacgacga cgtggccagc
       61 cgcgccaatg agcgcggcca gtcggccgat ttctgtctct agaacctcgc gggtcggctc
      121 caacagctcg tgggcgcgga cgggatcggt tggaggcacg tcggtcacgg cgccatcatc
      181 tctgagccgc gcccagtcgg cggcgtgttc gagcgtcttc atggccgaga atgcaactgt
      241 gacttcgttg gcggacttac tcgttgatgc aactcggctt ctagtcgttg agggcatcga
      301 gtacgaccct tcgagccgtc gcaagaccag agcactcttt cgcgacaccg acccggcgtc
      361 catcgcgcaa cttgtggcat cgctggccac aggcccctcg cccgacgacg tcgcgctcat
      421 gcagtggccg tcgatgacat tcgtcttgtt cgagggcgac cacgaacttg cgagagtgga
      481 atacctaggc ggatggctcc gatggagtgg atggcagggt gatcggccac tgatacgccc
      541 tgccgacctg gcctcgtggc tgacggctca cggcctcact gcgtgaatcc ggaactaccg
      601 gatctcggca ggttcgaaag tgcgtcatcg cggcttctta tagctttcgg ctagggccga
      661 gcccggcagg gtttggccta ggcgtccggc tcgtcgttct cattgcggct gcccacgctc
      721 gtcgtgtggc tcacatttgg cctgcgcgag ccggacgtcg tttcaacgag cgggagaggc
      781 tcaagaaggg tttgctctgc tcgaagaagc ggtgccctcc cagctcacac acatcaacac
      841 cgtgtcgaga tttgaggagt cgggatgggt gcagtggtga tcgggatgga cccgcataag
      901 aggaccgcga cgatcgaagt gatggacggc gacgagacga ttcttggtgg cggccggtat
      961 gccaccgacg tcgccggcta ccaggcgatg ctcgcgttcg gtcagcagtt cgctgatcgg
     1021 acgtgggcga tcgaaggctg taacgggatc ggtaaacaca tcgccgatcg gctcctggcc
     1081 gatggtgaac aggtagtgga tgtgccaccg aagctgtccg cccggacgcg ggtgttcgcc
     1141 accggccagg gccgcaaaac cgacgccacc gacgcgcact cggtcgcgtt ggtggggacc
     1201 aggatgagtg ggctgcgacc ggtcgtggcc gatgaggagc tcgccgtgtt gcggctgctc
     1261 gcggatcgtc gccgctcgct tggtcaagac cacactcgga tggtcgctca gctccatcgg
     1321 atactcctcg agctcattcc tggtggagcg aagaaacagc tgtccgcggc gcaagccaag
     1381 gtattgctgg ggaaagtccg cccccgcgac accgccggta agacccgcaa gcgcgtttgc
     1441 gcagagctgg tcgctgatct gcagcggatc catgagcgca cgaaagccgc cgacaaagaa
     1501 ctcaaagcgc tgctggtagc gaccggaacg acgttgatga cgctgcacgg catcggacca
     1561 tcaggtgcgg cgcggctgct cgtcgaggtc ggtgacatca cccgcttccc gacccgtgcg
     1621 cacttcgcct cgtggaccgg caccgccccg atcgacgcgt cgtctggtga tcacgtgcgg
     1681 caccggctgt ctcggggtgg gaaccggcag atcaaccgca ctctgcacat catggccgtc
     1741 gcgcaactac gcaaccccac gaagggacgc gcgtactaca accggaaaaa ggtggacggg
     1801 aaagcgccga tggaagcgat gcgctgcctc aaacgacgac tgtccgacgt cgtctaccgg
     1861 cagctactcg cggacgcgat ccggccgagt aaggcaggtc cgggaggaca ctcgggggcg
     1921 gctcttgact ccagcgcggt cgactcaaac cccgacatcg acgcttcgaa gaagtcactt
     1981 cccggacccg ccaacaccca cgttacccgc gcgaaaagcc ctcgaccaga ccgttcttcg
     2041 acccgttccc gacgccctgc aacagcaccg aaccgcagcc gtcgtcaagc
//
LOCUS       DATCBJ010000005         2100 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_515658, whole genome shotgun sequence.
ACCESSION   DATCBJ010000005 DATCBJ010000000
VERSION     DATCBJ010000005.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2100)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2100)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2100
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_515658"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..384
                     /locus_tag="VII50_00095"
     CDS             <1..384
                     /locus_tag="VII50_00095"
                     /inference="COORDINATES: protein motif:HMM:NF019040.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HEY5049270.1"
                     /translation="DFHADEKAAQSTLLGQLDAAVSGFLTTVAAGSHGLDVVTVLYSE
                     FGRRVAANGSQGTDHGTAGNIFVAGAAVKGGMFGDQPSLTDLAMDDLKYNVDFRSVYA
                     TVLEGALGAAADQVLGGSFPKLAFI"
     gene            533..1090
                     /locus_tag="VII50_00100"
     CDS             533..1090
                     /locus_tag="VII50_00100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5049271.1"
                     /translation="MPATKKLTIAALLAVTLSIAGCNSKKASSTDSGSPASTAAAAPA
                     ASAANSGASAPAAAATGAGAASSGGCPLTAAQVTTVMGATYGAPQNFNGICFYTGNES
                     LSIDVENASGIHSFDATLAAVKESQGTDTTTTIPGLGDKATGVGQEIVVAAGGKTIDI
                     RSADTSGLGWPKSIALAKLVIAGLR"
     gene            complement(1457..>2100)
                     /locus_tag="VII50_00105"
     CDS             complement(1457..>2100)
                     /locus_tag="VII50_00105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012691715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEY5049272.1"
                     /translation="DPAITRAIGSIPDDAWTTISYPNAVFDEQLQHWVSDAEVAEIPF
                     TAFTSKPRNQQVNARLIVRRVRDANPNDIVANAQGEVFPAWRHHAVFTDTPLPMLDAE
                     GDHRRHAIIEQVIADLKNGPLAHLPSGKFAANSAWLVLAAIAFNLTRAAGCLASKFHA
                     RATTATIRTQLISVPARVARSARRLTLRLPQRWPWQDAWETLFTASTGPPTNP"
BASE COUNT          338 a          649 c          731 g          382 t
ORIGIN      
        1 gacttccacg ccgacgagaa ggcggctcaa agcacactgc taggacagct ggacgccgcg
       61 gtgtcggggt tcttgaccac ggtcgccgct ggttcgcatg gcttggacgt cgtgacggtg
      121 ctgtactccg agttcggtcg gcgggtagcc gcgaacggca gccagggcac cgaccatggg
      181 acggcgggga acatcttcgt cgcgggtgcc gccgtgaagg gcggcatgtt cggcgaccag
      241 ccgagcctga ccgatctggc tatggacgac ctgaagtaca acgtcgactt ccgatcggtc
      301 tacgcgaccg tgctcgaggg tgcattgggg gcggcggccg accaggtgct tggcggcagc
      361 ttccccaagc ttgcgttcat ctgacgtctc gcgtgacggg tcgcgtcacg gctgccgccg
      421 tgctgcgctc gatctgccgc cctgatcgag ggtttgcccc gattccccag gctcgccgcg
      481 aacctaccgt cggcaatgtg aacgcttgcc cgacggcgac cctggagtcg acatgccagc
      541 aaccaagaaa ctgacgatcg cggcacttct cgccgtcacc ctgtctatcg ccggatgcaa
      601 ttcgaagaag gcgagctcga ccgattcggg tagtcccgcg tcaaccgcgg ctgcggcacc
      661 agcagcgtcg gcagccaact ccggcgccag cgcacccgcg gccgccgcaa ccggggctgg
      721 tgcggcctcg tccggaggct gcccgttgac cgcagctcaa gtcaccacgg tgatgggcgc
      781 cacctacggg gctccacaga acttcaatgg gatctgcttc tacacgggca acgagtcgct
      841 cagcatcgac gtcgagaacg cgtccggcat ccatagcttc gatgccacac tcgcggcggt
      901 gaaagagtct cagggaacgg acacgacgac aaccattccg ggccttggtg acaaagcgac
      961 cggcgtcggg caggagatcg tcgtcgcggc cggcggcaag accatcgaca tccgcagcgc
     1021 cgatacctcg ggactcggct ggcccaagtc gatcgcgttg gcgaagctgg ttatcgccgg
     1081 cctccgctag gtgaaccgcc actgaaaccg gccggatgcg ccgcgccgac gacgggcggg
     1141 gcggaacccc ggaccgtgat cggaccgaac gggatcgaac cggccaagag ttgcttgagc
     1201 atctggctcg cagtcagcac ggcatcgaag cacgggtgct gaccgcgcgg ctattcgcgt
     1261 caatgccaat tgaggacgtt tacagatgcc gtttgttttc gctccgcggc ttagcccgga
     1321 tccaccgtgg tctgatttcg tgggcgcgta gttgatccgg tggttgtgtc gcggcggcgg
     1381 ggtgtggctg actggccggt gtgcccggct tttccacttc gagtcctggt cgtggccgtc
     1441 gcggcctgtg aacggttcag gggttggttg gtggtccggt cgaggcggtg aacagcgttt
     1501 cccatgcgtc ttgccagggc cagcgttggg gcagtcgcaa cgtcagtcgt cgcgcggatc
     1561 gggcgacgcg cgccgggacg ctgatcagct gcgtccggat cgtggcggtc gtcgcgcgag
     1621 catggaattt cgaggcaagg cagccggcgg cgcgggtgag gttgaacgcg atcgcggcga
     1681 gcacgagcca tgcgctgttg gcggcgaact tccccgaggg caggtgcgcg agcggcccgt
     1741 tcttcagatc tgcgatgact tgttcgatga tcgcgtgccg gcggtggtcg ccttcggcgt
     1801 cgagcatcgg cagtggtgtg tcggtgaaca cggcatggtg gcgccaggcg gggaacactt
     1861 caccttgggc gttcgccacg atgtcgttcg ggttggcatc gcggacgcgg cgcacgatca
     1921 accgtgcatt cacctgctgg ttgcgcggtt tcgacgtgaa cgcggtgaac gggatttcgg
     1981 cgacctcggc atcggaaacc caatgctgga gttgttcatc gaagaccgcg ttcgggtagc
     2041 tgatcgtcgt ccacgcatcg tcggggatcg acccgatcgc ccttgtgata gctgggtcct
//
LOCUS       DATCBJ010000006         2549 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_220490, whole genome shotgun sequence.
ACCESSION   DATCBJ010000006 DATCBJ010000000
VERSION     DATCBJ010000006.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2549)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2549)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2549
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_220490"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(305..1585)
                     /gene="thrC"
                     /locus_tag="VII50_00110"
     CDS             complement(305..1585)
                     /gene="thrC"
                     /locus_tag="VII50_00110"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020660962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HEY5049273.1"
                     /translation="MSATQSPPAVPPHSTLLGSATSLSCRECGEQYPISPRYACEMCF
                     GPLEVAYDYAGVSRKSIEAGPHNIWRYKQLLPVPANVAETPNLEPGMTKLVLANNLAR
                     ELGMRKLWVKDDSGNPTHSFKDRVVAVALAAARELGFSVLACPSTGNLANAVAAAAAR
                     AGIKSVVFIPADLERQKVVTTAVYGGTLVAVEGNYDDVNRLASEIAGDEEDWAFVNVN
                     VRPFYAEGSKTLGYEIAEQLGWRLPSQVVIPIASGSQLTKVDKAFTEFGKLGLVDATP
                     YRVFGAQASGCSPVSAAFKAGYDVVQPVRPNTIAKSLAIGNPADGPYVLDVCRRTGGL
                     VEDVTDEEIVDGMRLLARTEGIFGETAGGVTIGVLRKLLASGQLDPDAETVVFNTGDG
                     LKTLDAVAPVVSPSVTIPPSIEAFRAYLDVAGLA"
     regulatory      complement(1599..1734)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            complement(1958..>2549)
                     /gene="otsB"
                     /locus_tag="VII50_00115"
     CDS             complement(1958..>2549)
                     /gene="otsB"
                     /locus_tag="VII50_00115"
                     /EC_number="3.1.3.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004805 - trehalose-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="trehalose-phosphatase"
                     /protein_id="HEY5049274.1"
                     /translation="NLLIVGHYGLETWTAAAGVVPVAGTDPRRSERIDHVRAALPDLL
                     RDVGAPPGTRVEDKGAAVAVHVRETADPHAALELLREPLRRLADSQGLRLEPGRLVLE
                     LRPAGTDKGSALSSLVHVFNARSVCYVGDDLGDIAAFDALHDLRSRGFAALAVFSGTP
                     DQPGLADLAARADVQLSGPAAVVDFLEALRSAFRSG"
BASE COUNT          440 a          871 c          823 g          415 t
ORIGIN      
        1 cggcgagctg gttcataccg cgctcgaggg cgcgacgagc agtctcgtca tacgcgatga
       61 tcttggacat gcggatggtc ctcccgggtc gggaaacgta gcgtgcggcc ggactcgacg
      121 cccgcgacgg ccgaccacgc ccaaccggtg tcatgtccgc cggctcggca gggtctcgaa
      181 ggcccggcca ttaacttgct gaactgtcac tcacgtgctc agagtgctag ctcatgttta
      241 gcactcaggc accccgagtg caaccgatga ccggtgaaca actccagcgt gtgcgcggcc
      301 cgcgctaggc caggccggcg acgtccagat aggcgcgaaa cgcttcgatg gacggcggga
      361 tggtgaccga cggcgagacg accggcgcga cggcgtcgag ggtcttcagc ccgtcgccgg
      421 tgttgaagac gacggtctct gcgtcggggt ccagctgccc tgacgcgagc aacttgcgca
      481 gcacgccgat cgtcacgccc cctgcggtct caccgaagat gccctcggtg cgggcgagca
      541 gccgcatgcc gtcgacgatc tcctcgtcgg tgacgtcctc gaccagcccg ccggtacgcc
      601 ggcagacgtc gagtacgtaa ggtccgtcgg ccgggttgcc gatggccagc gacttcgcga
      661 tcgtgttcgg ccgcaccggc tgcaccacgt cgtagccggc cttgaacgcg gccgacacgg
      721 gcgagcaacc agaagcctgc gcaccgaaaa ctcggtaggg ggtcgcgtcg acgaggccga
      781 gcttgccgaa ctccgtgaac gccttgtcga ccttcgtcag ttgcgaacct gacgcgatcg
      841 ggatgaccac ctgcgacggc aaccgccagc cgagctgctc ggcgatctcg tagcccagcg
      901 tcttcgatcc ctcggcgtag aagggccgca cgttcacgtt gacgaacgcc cagtcctcct
      961 cgtcgccggc gatctccgaa gcgagccggt tcacgtcgtc gtagttgccc tcgaccgcga
     1021 ccaacgtgcc gccgtaaacc gccgtcgtga cgaccttctg ccgctcaagg tccgccggga
     1081 tgaacaccac cgacttgatg ccggcccgcg ccgctgcagc ggccacggcg ttcgccaggt
     1141 tcccggtcga ggggcaggcg agcacggaga agccgagctc gcgcgcggcc gccagcgcga
     1201 ccgcgacgac tctgtccttg aacgagtgtg tggggttgcc gctgtcgtcc ttcacccaca
     1261 gcttgcgcat cccgagctca cgggcgaggt tgttcgctag caccagtttt gtcataccgg
     1321 gctcaagatt cggcgtctct gcaacgttgg ccggcaccgg cagcaactgc ttgtagcgcc
     1381 agatgttgtg cggtccggct tcgatcgact tccgactcac acccgcgtag tcgtaggcca
     1441 cctcaagtgg cccgaagcac atctcgcacg cgtagcgcgg actgatcgga tactgctcgc
     1501 cgcactcgcg acatgacagc gacgtggccg accccaacag ggtcgaatgc ggcgggacgg
     1561 cggggggcga ctgggtcgcg gacacggcga agcctctctc ctcatcttcc ccgcggacca
     1621 tccacgggac ggagttggca cctgtttcgc tactgcgaga cgttcctcac gtagtacgtc
     1681 gaaatggttg ccggggcttc aacgggccgg tccctctgcc cctcttgatg agcactgcaa
     1741 atcggtactg ctgccgaact gtcgacaagg gtagcgcgtc caggcatcgg ccgaacggct
     1801 gaccaaagtc ggccgttttt cggagagcca acgagtcatc cagggtttat cgtcatcgac
     1861 attggagggg aaccgcgggc ggggacatac cgggcaggaa tgttctcggc cgcggttttt
     1921 ttctgcccac gagcaggtca agcagggcgg acggcactta gccagacctg aaagcactgc
     1981 gcagcgcttc gagaaagtcg accaccgcag cggggccgct gagttgcacg tcagcgcgcg
     2041 cagccaggtc ggcaaggccc ggctgatccg gcgtgccgct gaacaccgcg agtgccgcga
     2101 atcccctcga ccgcagatcg tggagcgcat cgaacgctgc gatgtcaccg aggtcgtcgc
     2161 ccacgtagca caccgaccgt gcgttgaaca cgtgcacgag cgagctgagc gcgctcccct
     2221 tgtccgtacc ggcagggcgc aactcgagga ccagccggcc gggctcgaga cgtaacccct
     2281 gcgagtcagc aagccgccgc agcggttctc gcagcaactc cagcgcagcg tggggatctg
     2341 cagtttccct gacgtgcacg gcgaccgcgg cacccttgtc ctcgacgcgc gttcccggcg
     2401 gtgcgccgac gtcgcgcagc aagtccggta acgccgcgcg tacgtggtcg atgcgttctg
     2461 accgccgagg gtcggtgccc gccaccggca ccactccagc agccgccgtc cacgtctcga
     2521 gaccgtaatg gccgacgatc aacagattc
//
LOCUS       DATCBJ010000007         6184 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_441493, whole genome shotgun sequence.
ACCESSION   DATCBJ010000007 DATCBJ010000000
VERSION     DATCBJ010000007.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 6184)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6184)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6184
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_441493"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..149)
                     /locus_tag="VII50_00120"
     CDS             complement(<1..149)
                     /locus_tag="VII50_00120"
                     /inference="COORDINATES: protein motif:HMM:NF022629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="general stress protein"
                     /protein_id="HEY5049275.1"
                     /translation="MTFGDPIRLDPIHAAWNTVASFGTYEDAQEAVDRLSDSGFPVEN
                     IDIIGS"
     gene            complement(160..1590)
                     /locus_tag="VII50_00125"
     CDS             complement(160..1590)
                     /locus_tag="VII50_00125"
                     /inference="COORDINATES: protein motif:HMM:NF012503.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HEY5049276.1"
                     /translation="MSAQRATYDAALDRAVVHAKAWLDSLPDRDVPPRLSADEIAPSF
                     AGPLPDGPTDPAEVIELLAALAEPGLMAMPSGRFFGWVIGGALPAALAADWLTSAWDQ
                     NSTLRYATPAAAAAEEAAGGWLLDLLGLPVGADVGFVTGATMANFSGLAAARLQVLAH
                     VGWDVNTKGLNGSPPIRVIVGAERHSAVDVALRYLGLGEPTPVAADDQGRIQVDALAA
                     ALDAGSGPTIVCLQAGNVHSGAFDDIGEAARVAHEHGTPDARPWVHVDGAFGLWAAAS
                     PSLRLLVRGLADADSWATDAHKTLNVPYDCGIVMVARPAALRAALGVRASYLISSDGP
                     VGDPDEKVPELSRRARGIPVWAALRSLGRDGVADLVDGLVRHARAIAAGLETIDGAQV
                     LNDVVFTQVCVAFEDDERTRAVTAQLLADGTAWMSGSRWHGRDVLRVSVSNWSTDEGD
                     VEASVEAVRRAANAVRLASVGQGNSL"
     gene            1738..2991
                     /locus_tag="VII50_00130"
     CDS             1738..2991
                     /locus_tag="VII50_00130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5049277.1"
                     /translation="MATRDRGELPVTRPVRRLASAPAATVLGITAAAFAVAALVISVA
                     DHKAASAGIVLVPTTMFAAVGVVVARRQPANPIGWLLIFVSIFENMYAFVAAYVTFNH
                     WLGHGLNWPSFTALWFENSFWDLGLIVGLPALLLFPDGVVPSPRWRPTLRLYVVGGVL
                     VVISQCVAATILVRFHDLQVDANGQPVGTPKSAGFVAFLESGVAVLILLPLMVSWVVH
                     LAVAFRRSEGVLRQQLKWATAGALVLVVSLAVSVPLGNDTSAVAQVVSGLCLALFGAF
                     PLSLGLGILKYRLYDIDRLISRTLSYTVVTGLLIGVYVGVVVLTTNVLSFSSPVAVTA
                     STLAAAALFTPIRTAAQRLVDRRFNRARYDAEATVAAFSARLRDAVDLETVRLDLLAV
                     VSHAVEPTHASIWISPARATRAGSP"
     gene            complement(2954..3967)
                     /gene="hpnH"
                     /locus_tag="VII50_00135"
     CDS             complement(2954..3967)
                     /gene="hpnH"
                     /locus_tag="VII50_00135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009076658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosyl-hopene transferase HpnH"
                     /protein_id="HEY5049278.1"
                     /translation="MAVPMRQGARIGWYLFSQKLHRREKFPLIVELEPLFACNLKCNG
                     CGKIQQPHDLLRQRMPVERAIAAIEEAGAPMVSLAGGEPLMHPQIDVMVNELVKRKKY
                     VYLCTNAELVRKRWDKFNFKPSPYFAFTIHIDGLQERHDESVAKEGVFEEAIEAIKFL
                     KEKGFRVTTNSTFFNTDTPQNIIDVLNFLNDEVKVDQMMISPAYAYEKAPDQDHFLGV
                     TETRELFAKAFAGGNRKRWRLNHSPLFLDFLEGKVDFECTAWGIPSYSLLGWQRPCYL
                     MADGYVKTYKELIETTDWSKYGRGRDERCNNCMAHCGYEPTAVLATMSSIKESLRALR
                     EQG"
     gene            complement(3967..5466)
                     /gene="ispH"
                     /locus_tag="VII50_00140"
                     /gene_synonym="lytB"
     CDS             complement(3967..5466)
                     /gene="ispH"
                     /locus_tag="VII50_00140"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009076659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA];
                     GO_process: GO:0050992 - dimethylallyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HEY5049279.1"
                     /translation="MINLLIVTALRTEYAALAGRVPGATLARCGMGPERVNAWLPRLA
                     QLSPNAVVVAGVAGGLDPTLRPGDVVVATEVRDERGRIVQRAAAPLVAELRRMGLRVR
                     TGPMVSCDHIVRGAAARARLAATGAIAVDMESAAIVRAAAGVPTAVVRVIVDTAYSPL
                     ASLNTATAGARALLTLHKIGPALRLWADLVGPRRVLLAAPRSFCAGVERAIDIVELAL
                     QRYPRPVYVRRQIVHNAHVVADLQRQGAVFVEELDQVPDGTTVVFSAHGVAPAVRAEA
                     ARRGLNVIDATCPLVAKVHSEARRFAARGDTVLLIGHAGHDEVEGTLGEAPGKIRLVQ
                     SAADAERVSAEDPEHVSFLMQTTLAVDDAMETVDVLRRRFPSIESSATDDICYATTNR
                     QQAVRAIAAESDVMMVLGSANSSNSVRLVEVAERCGVPAHLVDDANDILPEWLDGAST
                     IGITAGASAPPHLVDEVIETLRALGPVDVIERAVTDEDVTFSLPKGVAG"
     gene            complement(5463..>6184)
                     /locus_tag="VII50_00145"
     CDS             complement(5463..>6184)
                     /locus_tag="VII50_00145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="prenyltransferase/squalene oxidase
                     repeat-containing protein"
                     /protein_id="HEY5049280.1"
                     /translation="GNDAKPAIERGLAWLTGMQSRDGGWGAFDADNTRALINKLPFCD
                     FGAVIDPPSADVTAHVVEMLGHSGMAQTEAARRGVRWLLDAQEEDGSWFGRWGANYVY
                     GTGAVLPALAAVGADRDSIPVRRAVHWLEKHQNPDGGWGEDLRSYTDAEWRGKGESTP
                     SQTAWALLGLLAVDPGNDAVIRGVDWLVRTQRSDGGWDEDLYTGTGFPGDFYINYEMY
                     RVVFPLSALGRYLTTDKAGSP"
BASE COUNT          995 a         2125 c         1993 g         1071 t
ORIGIN      
        1 gagccgatga tgtcgatgtt ctcgaccggg aatccggaat cggacagccg atcgacggct
       61 tcctgagcat cttcgtaggt gccgaaactg gcgaccgtat tccaggccgc gtggattgga
      121 tcgagccgga ttgggtctcc gaacgtcatc gtcgtctcct caaagactgt tcccctgccc
      181 aacgctcgcg agccgtacag cgttcgcggc ccggcgaaca gcctcaaccg acgcctcaac
      241 gtcgccttcg tccgtcgacc agttgctgac cgatacccga agcacgtcgc gtccatgcca
      301 ccgcgagccg gacatccatg ccgtcccgtc tgccagcagt tgggccgtta ccgcccgggt
      361 acgctcgtcg tcctcgaaag cgacgcagac ctgggtgaag accacgtcgt tgagcacctg
      421 cgcaccgtcg atggtctcga gcccggcggc gatcgcccgc gcgtgccgca ccaacccgtc
      481 gaccaggtcc gcgacaccgt ctcggccaag tgatcgcaac gcggcccaca ccgggatgcc
      541 tcgcgcccgc cgcgacaact ccggaacctt ctcgtccgga tcgcctaccg gtccgtccga
      601 ggagatcaaa taacttgcgc ggacgccgag tgctgcccgc agtgcagctg gtcgggcgac
      661 catcacgatg ccacagtcat agggaacgtt gagggtctta tgggcatcgg tggcccagga
      721 atcagcgtcc gcgagacctc ggacaagaag ccgcaggctc ggcgacgccg ctgcccacag
      781 cccgaaggcg ccgtcgacgt gaacccaggg ccgcgcatcc ggcgtcccgt gctcatgcgc
      841 gacgcgggcg gcttcgccga tgtcatcgaa cgcgccggaa tgcacgttgc cggcctgcag
      901 gcagacgatc gtggggccac tcccggcgtc gagtgccgct gcgagggcat cgacctggat
      961 ccggccctgg tcgtcggcgg ccaccggtgt cggttcacct aggccgaggt aacgcagcgc
     1021 tacgtcgacg gcgctatgcc gctcggcgcc gacgatcacc cgaatcggcg ggctgccatt
     1081 cagacccttc gtattaacgt cccagcccac gtgtgcgagc acctgcagcc gagcggctgc
     1141 cagaccggag aagttggcca tcgtcgcacc ggtgacaaag ccgacgtcgg cgccgacggg
     1201 cagcccgagc aagtcgagaa gccagccacc cgccgcctct tccgcggcag cagcggcggg
     1261 cgtcgcgtat cgcagcgtgc tgttctggtc ccaggcactg gtgagccagt cggccgcgag
     1321 cgccgccggc agcgcgccgc cgatcaccca gccgaagaac cggccgcttg gcatcgccat
     1381 gaggccgggt tccgccagcg cggcgagcag ctcgataacc tccgcgggat cggtcggccc
     1441 gtccggaagc ggaccggcga acgacggcgc gatctcgtct gccgacaggc gcggcggcac
     1501 gtcgcgatct ggcagcgagt cgagccacgc cttcgcgtgg acgaccgcac gatcaagcgc
     1561 agcatcgtag gtcgcgcgtt gtgcgctcat cgcttaatca ccatgccccc atcatcgttc
     1621 gcccagagcc cgccgtcgac acccttttac gtcgcccgca ggcggttacc aaccgcgctc
     1681 gaacagccgt gcgcgtaacg atctcggaac gccggcggtc taacctgctc cctatcgatg
     1741 gctacgagag atcgcgggga gttgccggtg accaggccag tgcggcggct ggcatccgcg
     1801 ccggccgcca ccgtgctggg catcaccgcg gcggcgttcg ccgttgctgc tcttgtcatc
     1861 tctgtggccg atcacaaggc cgccagcgct ggcatcgtgt tggttcccac gacaatgttt
     1921 gccgcggtcg gagtggtcgt cgcgcgccgc cagcccgcca accccatcgg gtggctgctg
     1981 atcttcgtat cgatatttga aaacatgtat gccttcgtgg cggcctacgt gacgttcaac
     2041 cactggctcg gccacgggct caactggccg tcctttactg cgctgtggtt tgagaactcg
     2101 ttttgggatc tcggattgat cgtcggcttg cccgcactgc tgctctttcc cgacggcgtc
     2161 gtgccgtcac ctcgctggcg cccgacgtta cgtctgtacg ttgttggtgg cgtgcttgtg
     2221 gtgatctcgc agtgcgtcgc cgccaccatc ctggtgcgct ttcacgattt gcaggtggac
     2281 gcgaacggcc aaccggtggg cacgcccaag tcagctggct tcgtggcgtt ccttgagtcg
     2341 ggcgtggcgg tgctcatact gctcccgctg atggtgtcgt gggtggtcca cctcgcagtc
     2401 gcctttcgcc ggagcgaggg cgtcttgcgg cagcaactca agtgggcgac tgccggcgcc
     2461 ctcgtcttgg tagtgagcct cgcggtgtca gtgccgctgg gcaacgacac gtcggcggtg
     2521 gcacaggtgg tcagcggcct ctgcctcgca ttgtttggcg cattcccgct gagcctggga
     2581 ctaggcattt tgaaataccg gctctacgac atcgatcgcc tgatcagccg cactctgtcg
     2641 tacacggtgg tgaccggcct gctgatcggc gtgtacgtgg gagttgtggt cctcacaacc
     2701 aacgttctct cgttctcgtc acctgtcgcg gtcaccgcgt caaccctcgc cgcagcagcg
     2761 ctgttcaccc cgatccgcac agcagcgcag cggctggtcg atcgccggtt caaccgcgcg
     2821 aggtacgacg ctgaggcgac cgtcgcggca ttttccgctc gcttgcgcga cgcggtcgat
     2881 ctcgagacgg tacgtctcga cctgctcgcc gtcgtcagtc acgccgtcga gcccacccac
     2941 gcctcgatct ggatcagccc tgctcgcgca acgcgcgcag gctctccttg atcgagctca
     3001 tcgtcgcgag caccgcagtg ggttcgtacc cgcaatgcgc catgcagttg ttgcaccgct
     3061 cgtcgcggcc gcgaccgtac ttgctccagt cggtggtctc gatgagctcc ttgtacgtct
     3121 tcacgtagcc atcggccatc aggtagcacg ggcgctgcca gcccagcaac gagtacgagg
     3181 gaattcccca cgcggtgcac tcgaagtcga ccttgccctc gaggaagtcg aggaacaggg
     3241 gcgagtggtt caaacgccag cgcttgcggt taccgccggc gaacgccttc gcgaacagct
     3301 cgcgagtctc ggtcacgccg aggaagtgat cttgatctgg cgccttctca tacgcatacg
     3361 ccggcgagat catcatctgg tcgaccttga cctcatcgtt gaggaagttc agcacgtcga
     3421 tgatgttctg cggcgtgtcg gtgttgaaga acgtcgagtt cgtcgtgacc cgaaaaccct
     3481 tttccttcag gaacttgatc gcctcgatcg cttcctcgaa cacgccctcc ttggcgacgg
     3541 attcgtcgtg acgttcctgc agtccatcga tgtggatcgt gaaggcgaag tacggcgacg
     3601 gcttaaaatt gaacttgtcc caacgcttgc ggacgagttc ggcgttggtg cacaggtaga
     3661 cgtacttctt gcgcttgacg agttcgttga ccatcacgtc gatctgcggg tgcatcagcg
     3721 gctcaccacc ggcgagcgac accatcggcg cgccagcctc ctcgatcgcc gcgatcgctc
     3781 gctcgaccgg catgcgctgg cgcagcaggt catgcggctg ctggatcttg ccgcagccgt
     3841 tgcacttgag gttgcaggcg aacagcggct cgagctcgac gatcagcggg aacttctcgc
     3901 gtcggtgcag cttctgggag aacaggtacc agccgatacg agcgccctgg cgcatgggaa
     3961 cggccatcag ccggcaaccc ctttcggtag cgagaacgtg acgtcctcat ctgtaacggc
     4021 acgctcgatg acgtcgacgg ggccaagcgc ccgaagcgtt tcgatgacct catcgacgag
     4081 atgtggtgga gccgacgctc cggcggtgat gccaatcgtc gacgcgccat cgagccattc
     4141 gggcaggatg tcgtttgcat cgtcaacgag atgcgcgggg actccgcaac gctcggctac
     4201 ttcgacgagc cgcaccgagt tcgaggagtt cgccgacccg agcaccatca tgacgtcgga
     4261 ctctgcggcg atcgcgcgca ccgcctgctg gcggttcgtg gtcgcgtagc agatgtcatc
     4321 ggtcgctgac gactcgatcg aggggaaccg tcggcgcaac acgtcaaccg tctccatcgc
     4381 gtcgtcgacg gcgagcgtcg tctgcatcag gaacgacacg tgctcggggt cctcggcgct
     4441 gacccgctcg gcgtccgcgg cgctctggac gagccggatc ttgccgggag cctcgcccaa
     4501 ggtgccttcg acctcgtcgt gacctgcgtg cccgatgagc agcacagtgt caccgcgcgc
     4561 agcgaagcgg cgagcctcgc tgtggacctt cgccaccaac gggcaggtgg cgtcgatgac
     4621 gttcagcccg cggcgcgcgg cctcagcccg gacggcgggt gcgacaccgt gtgccgagaa
     4681 caccaccgtg gtcccgtcgg gcacttggtc gagttcctcg acgaacaccg cgccctgccg
     4741 ctgaaggtcg gcgaccacgt gcgcgttgtg cacgatctgg cggcgcacat agacgggtct
     4801 cgggtaacgc tgcagggcca gctcgacgat gtcgatcgca cgctcgacgc cggcgcaaaa
     4861 cgatcgaggt gcagccagca ggacgcgtcg ggggccgacg agatcggccc aaagccgcag
     4921 cgccggaccg atcttgtgca gcgtcagcag cgcacgtgcg ccagcggtcg cggtgttcag
     4981 gcttgccagc gggctgtagg cggtgtcgac gatgacccga acgacagcag tcgggacgcc
     5041 ggccgctgcc cgcacgatcg ctgcggactc catgtcgact gcgatcgcgc cggtggcggc
     5101 cagccgcgct cgagcggccg ccccgcgcac gatgtggtca cagctgacca tcgggccggt
     5161 tcggactcgc aaccccatcc gacgcaactc cgcgacaaga ggcgcggcgg cgcgctgcac
     5221 gatccgacca cgctcatcgc gcacttcggt agccacgacg acgtcaccgg ggcgcaacgt
     5281 cgggtcgagg ccgcccgcaa cgcccgcaac gaccaccgcg ttcggcgaaa gctgtgcgag
     5341 ccgcggcagc cacgcgttca cccgctccgg ccccatgccg cagcgggcca gggtcgcgcc
     5401 aggcacccgg cctgccaacg cggcgtactc ggtgcgcagc gcggtcacga tgagcaggtt
     5461 gatcacggcg acccggcctt gtcagtcgtc aggtagcggc cgagcgcgct gagcgggaac
     5521 accacgcggt acatctcgta gttgatgtag aagtcgccgg gaaagccggt gccggtgtag
     5581 aggtcttcgt cccagccgcc gtcacttcgc tgggtgcgta ccagccagtc gacgccgcgt
     5641 atgacggcat cgttgccggg gtcgacggcg agcagaccga gcagcgccca agccgtttgc
     5701 gacggggtcg actcgccctt gcctcgccac tccgcgtcgg tgtacgagcg caggtcctcg
     5761 ccccacccgc cgtccggatt ctgatgcttc tcaagccagt gcacggcgcg ccgcaccggt
     5821 atcgagtcgc gatcggcacc gacggcggca agtgcaggca ggacggcgcc ggttccgtac
     5881 acatagttcg cgccccatcg gccgaaccag gaaccgtctt cctcctgcgc atcaaggagc
     5941 caccggacgc cgcgtcgcgc cgcctctgtc tgcgccattc cgctatgccc gagcatctcg
     6001 accacgtgcg cggtgacatc agcggacggc gggtcgatca ccgcgccgaa gtcgcagaag
     6061 ggcagcttgt tgatgagcgc gcgggtgttg tccgcgtcga acgcgcccca gccgccgtcc
     6121 ctggactgca tgccggtcag ccaggccagc ccgcgctcga tggcgggctt ggcgtcgttg
     6181 cccg
//
LOCUS       DATCBJ010000008         2176 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_404049, whole genome shotgun sequence.
ACCESSION   DATCBJ010000008 DATCBJ010000000
VERSION     DATCBJ010000008.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2176)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2176)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2176
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_404049"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..450)
                     /locus_tag="VII50_00150"
     CDS             complement(<1..450)
                     /locus_tag="VII50_00150"
                     /inference="COORDINATES: protein motif:HMM:TIGR01470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEY5049281.1"
                     /translation="MNAGYPLLLDVAGRAVLVVGAGPVGARRAIGLADAGALVTVVAP
                     AADDALSGDDRITLRRRAYEASDLDAVWLVHACTGDDAADSQVAADADAARVWCVRAS
                     SAPSSAAWTPAVARVDDLIVAVNAGGDPGRAAAVRDAVRLLLETGELP"
     gene            complement(450..1772)
                     /locus_tag="VII50_00155"
     CDS             complement(450..1772)
                     /locus_tag="VII50_00155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HEY5049282.1"
                     /translation="MSVIDNAAMLAATPVPALDPDVEMLRFATAGSVDDGKSTLVGRL
                     LHDSKSVLTDTLEAVERASRDRGTDGVELALLTDGLRAEREQGITIDVAYRYFTTSKR
                     KFVLADTPGHVQYTRNMVTGASTAELVVILIDARKGIVEQTRRHLAVAALLDVRHLVL
                     AVNKMDLVDFDEATFLAIAEEFAAAAQSFGVRHHVVIPVSAHLGDNVVERSTSMPWYA
                     GPTLLEHLEAVPVGFDAWIQPARLPIQYVIRSTDYRGYAGQLASGVIEIDDIVVIMPS
                     GMRTTIVGIDTFDGEVALAHAPQSVVVRLADDIDVGRGDLIVHPRQEPRVVREFEATV
                     CQLSDRPLSAGDRVLIKHTTKVVKAVVASVNHRLDVVTLEHDAADSLGLNEIGGVTLR
                     VAGPLAVDDYADDRLTGSFLVIDEHDGQTLAAGLVGDPIRRTPTGGES"
     gene            complement(1776..>2176)
                     /locus_tag="VII50_00160"
     CDS             complement(1776..>2176)
                     /locus_tag="VII50_00160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002855962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase family
                     protein"
                     /protein_id="HEY5049283.1"
                     /translation="YNGRHKPGEHVRVFPLSNWTELDVWQYVEQESIELPAIYLAHER
                     DVFLRAGMWLTAGEWGGPKAGEPTETKTVRYRTIGDMSCTGAVDSDARDVAQVINEIA
                     VATITERGATRADDKASEAAMEDRKREGYF"
BASE COUNT          382 a          750 c          695 g          349 t
ORIGIN      
        1 cggcagttcg ccggtctcga gcaacaggcg caccgcatca cggacagccg ccgcccgacc
       61 ggggtcaccg cccgcgttga cggcaacgat caggtcatcg acccgcgcga cggccggcgt
      121 ccatgcagcg gacgacggcg ccgacgacgc gcgcacgcac cacacccggg ccgcatcagc
      181 atcggcggca acctgagagt cggcagcgtc gtcaccggtg caggcgtgca ctaaccacac
      241 cgcgtcgagg tccgaggcct catatgcgcg ccgcctcaag gtgatgcggt cgtcgccgga
      301 cagcgcgtcg tccgccgccg gcgcgacaac ggtgaccagc gcacccgcgt ccgccaggcc
      361 gatcgcgcga cgggcaccaa ccggacccgc tccgaccacc agcaccgccc gccccgcgac
      421 gtcgagaagc agcggatagc cggcgttcat cacgactcgc caccagtggg cgtgcggcgg
      481 atcgggtcgc ctaccaaacc ggctgccagc gtttgcccgt cgtgctcgtc gatcaccaga
      541 aagctgccgg tcaaccggtc gtcggcgtag tcgtcgacgg cgagtggacc ggctacccgc
      601 aaagtgacac caccgatctc gttcaggccg agcgaatcgg cggcgtcgtg ctcaagcgtc
      661 acgacgtcga ggcggtggtt cacgcttgcc acgacggcct tcacgacctt cgtggtgtgc
      721 ttgatcagca cccgatcgcc tgcactgagc ggacgatcag acagctggca cactgtcgct
      781 tcgaactcgc gcacgacgcg tggttcctgg cggggatgca cgatgaggtc gccgcgcccg
      841 acgtcgatgt cgtcggcgag gcgcaccacg acggactgcg gcgcgtgcgc cagcgccacc
      901 tcgccgtcga atgtgtcgat gcccacgatg gtcgtgcgca tcccggacgg catgatgacg
      961 acgatgtcgt cgatctcgat gacaccggac gcgagttgcc ctgcatatcc gcggtagtcg
     1021 gtcgatctga tgacgtattg aatcggcagc cgagcgggct ggatccacgc gtcgaagcca
     1081 accggcacgg cctcaagatg ctcgagcagg gtcggcccgg cgtaccacgg catcgaggtc
     1141 gaacgctcga cgacgttgtc gcccagatga gcggacaccg ggatcaccac atggtggcga
     1201 actccgaatg attgagccgc cgctgcgaat tcctccgcaa tagcaaggaa agtagcctcg
     1261 tcgaagtcga cgagatccat cttgttcacg gcaagaacaa gatgccggac gtcgagtagt
     1321 gccgccactg ccaagtgccg ccgcgtctgt tcgacaatgc ccttgcgcgc gtcgatcaag
     1381 atgacgacga gttcagcggt ggatgctccg gtcaccatgt tgcgggtgta ctggacatgg
     1441 ccaggcgtgt ccgcgagcac gaacttgcgc ttcgacgtcg tgaagtagcg gtacgccacg
     1501 tcgatcgtga tgccctgctc gcgctcggcc cgcagcccat cggtcaacag ggcgagttcg
     1561 acgccgtcgg tcccgcgatc gcgactcgcc cgctcgacgg cttcgagggt gtcggtcagc
     1621 actgacttag agtcatgcag cagccgtccg actagggtcg acttgccgtc atcgaccgaa
     1681 ccggccgtcg caaagcgcag catctcgacg tccggatcaa gcgcgggcac gggcgtcgcg
     1741 gcgagcatgg cggcattgtc tatgacggac atttcctaga agtaaccttc ccgcttgcga
     1801 tcctccatcg ccgcctccga tgccttgtcg tcggcacggg tcgcaccacg ctcggtgatc
     1861 gtggcgaccg cgatctcgtt gatgacttgg gcgacgtcgc gcgcatcgga gtcgaccgcg
     1921 cccgtgcaac tcatgtcgcc gatcgtgcga taccgcacgg tcttcgtctc ggtcggctcg
     1981 ccggctttcg ggccgcccca ctcccccgcg gtcagccaca tgccggcgcg caggaacacg
     2041 tcgcgctcat gagcgagata gatcgccggc agctcgatcg actcctgctc gacgtactgc
     2101 cagacgtcaa gctctgtcca gttcgacagg ggaaagacgc gcacgtgctc gcccggcttg
     2161 tggcgaccgt tgtaca
//
LOCUS       DATCBJ010000009         2095 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_145801, whole genome shotgun sequence.
ACCESSION   DATCBJ010000009 DATCBJ010000000
VERSION     DATCBJ010000009.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2095)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2095)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2095
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_145801"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..199
                     /locus_tag="VII50_00165"
     CDS             <1..199
                     /locus_tag="VII50_00165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5049284.1"
                     /translation="AILGSALGAAIGLIAASYAFGLSALVVVGALGAMGTGAAAALLG
                     TGAVLAVTGGRSLTPALARDE"
     gene            196..903
                     /locus_tag="VII50_00170"
     CDS             196..903
                     /locus_tag="VII50_00170"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY5049285.1"
                     /translation="MTVEIRGAEVCFRQVRKTYRTGAGVSFQAIAGVNLEVPAGQSVA
                     ITGRSGSGKSTLLSLAGGMDVADAGTVSVDERNLGSFSNRERTVFRRGVGFVFQRFHL
                     LPALTAWDNVAAPVVPFRTTFDKRARARELLAAVGLQGREHDVPARLSDGEQQRVAIA
                     RALIGEPRLLLADEPTGNLDSVVGSQVLDLLFSMHREHGVTLIIATHDPAVASRCERV
                     VTLSDGRITGEYRNEAV"
     gene            complement(923..1855)
                     /locus_tag="VII50_00175"
     CDS             complement(923..1855)
                     /locus_tag="VII50_00175"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEY5049286.1"
                     /translation="MRGLEIGPLASPRVRKDEGPVRYVDHASAAELREKYATDEGMQD
                     RLDRIVDVDYVLGVDTTISEAVAKDVPFDYVLASHVIEHIPDPIGWMDDLTRVLRPGG
                     VLSLVIPDKRYCFDINRSLTEISDFVDGNLRKLRQPSFRQAFDFYSKAIGGTVDTAAV
                     WAGTADYSLAVRQDFADPDAAALEACRHMLISDEFVDVHCHVFTPDSFVRLVEKMARL
                     DLIDYEIAAFHPTEINNFEFSVSLRLLDTSMGRDAMKELQLASVARALDANARPATAQ
                     SGLVRMEVSKQEQRLIDMKRRSLARARAVLKRGR"
BASE COUNT          365 a          661 c          673 g          396 t
ORIGIN      
        1 tgccatcctc ggatcggcgc ttggcgcggc gatcgggttg atcgcggcca gctacgcatt
       61 cggactctca gcgctcgtcg tcgtgggagc actcggcgcg atgggcaccg gtgcggccgc
      121 cgcccttttg ggcaccggag cggtgctcgc tgttaccggt ggccgatcgc tgacgccggc
      181 gctggcgagg gacgaatgac ggtcgagatt cgcggtgcag aggtgtgctt ccgacaggta
      241 cggaagacct accgtaccgg ggcgggcgtg tcctttcagg cgatcgccgg tgtgaacctg
      301 gaggtgccgg cgggtcagtc cgttgcgatc accggccgct cgggctccgg gaagtcaacg
      361 ctgctcagtc tcgcgggcgg catggacgtc gcggacgcgg ggaccgtcag cgttgacgag
      421 cggaatctcg gatcgttcag caaccgcgag cgaacggtat tccggcgcgg agtcgggttc
      481 gtcttccagc gcttccacct cctgccagcc ttgaccgcgt gggacaacgt cgccgcgccg
      541 gtggtgccgt ttcgcacgac gttcgacaag cgtgcgaggg cccgcgagtt gctcgcggcc
      601 gtgggtctcc aggggcgtga gcatgacgtg ccggccaggc tctcggacgg ggaacagcag
      661 cgcgtcgcca tcgcgcgcgc cctcattgga gaacccaggc tccttctcgc cgacgagccg
      721 acaggcaacc tcgacagcgt ggtggggagc caggtgctcg acctcctgtt ctccatgcat
      781 cgcgagcacg gcgtcacgtt gatcatcgcg acgcacgatc cggcggtagc ttcgcgttgc
      841 gagcgcgtcg tcacgctcag cgacggcaga atcaccggtg aataccgcaa cgaggctgtg
      901 tagcagttcc cgccaacgcg ctttagcgac cccgcttgag aacggcccga gcacgagcga
      961 gagagcgtcg tttcatatcg atgagcctct gctcctgctt gcttacctcc atccgtacca
     1021 accccgactg agccgtcgcc ggccgtgcgt tcgcatcgag cgcgcgcgcc actgatgcaa
     1081 gctgcaactc tttcatcgca tcgcgaccca tggaggtgtc aagcaaacgc agactcacgg
     1141 agaactcgaa attattgatc tcggtgggat ggaatgcagc tatctcgtag tcgataaggt
     1201 cgagccgggc catcttctca acgagtcgaa cgaatgagtc gggcgtgaag acatggcaat
     1261 gcacgtcgac gaactcgtcg ctgatcagca tgtggcggca ggcctccagc gcagccgcgt
     1321 ctgggtcagc aaaatcctgg cgaacggcaa ggctgtagtc ggcggtgccg gcccataccg
     1381 ccgccgtatc gactgtgccg cctatcgcct ttgagtagaa gtcaaaggct tggcggaaac
     1441 tgggctgccg caacttgcgt aggttgccat ccacaaagtc gctgatctcg gtgagcgacc
     1501 gattgatgtc gaagcaatac cgtttgtccg ggatgacgag ggacaggact ccgcccggtc
     1561 gcaagacgcg cgtcaggtcg tccatccacc caatcggatc ggggatatgt tcgatcacgt
     1621 gtgacgcgag cacatagtcg aagggcacat ccttcgccac cgcctcggag atcgtcgtgt
     1681 ccacgccaag gacgtagtca acatcgacga tcctgtcaag tcgatcttgc atgccttcgt
     1741 cggtcgcgta cttctctctg agctctgcag cgctggcgtg atccacatag cgcaccgggc
     1801 cttcgtcctt ccgcactcga ggagacgcga gcggccctat ctccagcccg cgcatgctcg
     1861 cgacgtcgat cgcggctagt tcaggtcgca tgtgtcggta gtgtgttcct gtcctgaatt
     1921 gcggtatgca aggatctggt ggcgatctgt ctgtcagggg ccgacgcaac cacggactct
     1981 acccctccgg cgacctcgct gacgcgcggg tattggctga tgcagcttga acgggcagat
     2041 cgacaacacg gccgtttctc acggtcttct catccgagcg ccccgcaccg actga
//
LOCUS       DATCBJ010000010         3132 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_403874, whole genome shotgun sequence.
ACCESSION   DATCBJ010000010 DATCBJ010000000
VERSION     DATCBJ010000010.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 3132)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3132)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3132
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_403874"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..479)
                     /locus_tag="VII50_00180"
     CDS             complement(<1..479)
                     /locus_tag="VII50_00180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HEY5049287.1"
                     /translation="MRVALDATPLLGTRTGIGHYVAHLAAALAKEPELDVVLTAFTWR
                     GQSELRGMQLDISEASEAGHPEYRVHSRRAPARLLRVAWSRSSLPPVEMFSGAVDVFH
                     GTNFVLPPTRHAAGVVTIHDLGFVLHPETVDRTSLAYQRLVPRALECARVILTPSQAA
                     "
     gene            649..1446
                     /locus_tag="VII50_00185"
     CDS             649..1446
                     /locus_tag="VII50_00185"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY5049288.1"
                     /translation="MAEMWRYRGLIGNFASRELKSKYKHSVLGWTWSLINPAATLAVY
                     TLVFSTIFRATPPKAHNGMTTYVIYLFIALVAWNFFSNVVNGSMAALIGAGPLLKKIY
                     FPPYAPVIGNSFSALVQTFIESAVLLVVLCGINNMSWSMFLTPVILLLLLVFASGIGL
                     MFALFNVYYRDVSYLVSIVMQLLFYSTPVIWAPSQLLVRKHGHFAIEVLSKSPMYQYV
                     EGFRDALWTGTMPALSRWLWMVASAIVSITVGLFVFNRGSRDVGEEL"
     gene            1443..2711
                     /locus_tag="VII50_00190"
     CDS             1443..2711
                     /locus_tag="VII50_00190"
                     /inference="COORDINATES: protein motif:HMM:NF025877.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY5049289.1"
                     /translation="MTTLSSDRRPPAVVVDHVTKEFKLYKDRRTSLKERFTKRRPSRF
                     DMFRALDDINLNVPAGSTYGLIGSNGSGKSTLLKLMANIHRPTSGSITHSGRISALLE
                     LGAGFHPELSGRDNVYLNGSILGMTRKQVDAAMDAIIDFSGIEEFIDTPVKVYSSGMY
                     VRLGFSIAVNLDPEILIIDEIIAVGDEEFQRRCFDYLYELRKRGVTIIFVSHSLPLVQ
                     TLCDRAAWIEKGVLRAEGSALEVVDAYVATVNRAERVRLDSEGHGRADGDLERRGGGE
                     ATVTAVTFIGSDGEPTLAATAGERLTLRIAYQASTALDDAVFGLAFHTESGVLVSGPN
                     SEFGGIHSGRIHGPGHIDYTVDSLALTPATYLVSAAITDITLLHVFDYRDRAFTLAVQ
                     PGKSPDRNGVVTLDGHWAPPISGTGTEFSQ"
     gene            2708..>3132
                     /locus_tag="VII50_00195"
     CDS             2708..>3132
                     /locus_tag="VII50_00195"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEY5049290.1"
                     /translation="MRVNLGCGQAYMEGWTNVDASPDVKADIHLDAAEFVRQYGDQVD
                     EVYMGHVFEHLMPGYAASLLRLLNERLRPGAVISVVTPDMRAIFEAYLAGEIDNDKLN
                     ASFIYSYVQPSHHVWCYDEATLLELFRRAGLADAEPITDV"
BASE COUNT          550 a          992 c          980 g          610 t
ORIGIN      
        1 gcagcttgcg agggcgtgag gatgacccgc gcgcattcga gcgccctcgg caccaaccgc
       61 tggtaggcca gcgatgtgcg gtcgaccgtt tctgggtgca agacaaatcc gagatcgtgg
      121 atcgtcacaa cgccagccgc atggcgggtc ggcggcagca caaaatttgt gccatggaaa
      181 acatcgacgg caccgctgaa catttccacc ggaggcaggc tgctgcgact ccacgcaacc
      241 cgcagcagtc gtgccggcgc gcgcctcgag tgcactcggt attcggggtg gccggcctcg
      301 ctggcctcac tgatgtcgag ttgcatgcca cgcaattcgg actggccacg ccaggtaaag
      361 gcggtcagga cgacgtcgag ctcgggctcc ttggcaaggg cggcggccag gtgtgcgacg
      421 tagtggccga tgccggtgcg ggtgccgagc aacggggtgg cgtccagcgc gactcgcacc
      481 tcggcatgtt cgcacaggtc gcggtgacac gggcgctcgc gcgcggtaaa ccggctggcg
      541 cgcaggggtg acgtaacatg ggacgtcgcc gtgagcccag gccccgccac acctgcgccg
      601 tccatgcggc cgcccaaccc ggaagcgagt ccacctgccg tgacccagat ggcggagatg
      661 tggcgctatc gcggacttat cggcaacttc gccagtcgcg aactgaagtc gaagtacaag
      721 cacagcgtgc tcggctggac ctggtcgctc atcaacccgg ccgccaccct cgccgtctac
      781 acgctcgtat tttcgaccat cttccgtgca actccgccca aggcgcacaa cgggatgacg
      841 acgtacgtca tctacctctt catcgcgctc gtcgcctgga acttcttcag caacgtggtg
      901 aacggcagta tggcggcact catcggggca ggtccgctac tgaagaagat ctacttccct
      961 ccatacgcgc cagtgatcgg caactcgttc tcggcattgg ttcagacgtt catcgaatcc
     1021 gcggtcctgc tggtcgtact gtgcgggatc aacaacatgt cgtggtcgat gtttctgaca
     1081 ccggtcatcc tgttgctctt actggttttc gcctcgggta tcgggctgat gtttgcgctc
     1141 ttcaatgtgt attaccgcga cgttagctat ctcgtcagca ttgtcatgca gttactgttc
     1201 tactcgacgc cggttatctg ggctccgagc caattgctgg tacggaagca tgggcatttc
     1261 gcgatcgagg ttctctcgaa gagtccgatg taccagtacg tcgagggctt ccgcgatgcc
     1321 ctgtggacag gcacgatgcc ggcgctatct cgatggttgt ggatggtggc gtcggcgatc
     1381 gtcagcatca cggtggggtt gttcgtcttt aatcgtggct cccgcgacgt cggcgaggaa
     1441 ctgtgaccac gctgtcctcc gaccgtcgcc cgcccgcggt ggtcgtcgac cacgtcacca
     1501 aggagttcaa gctctataag gatcggcgta ccagcttgaa agagcggttc accaagcgtc
     1561 gcccgagtcg cttcgacatg ttcagggcgc tcgacgacat caacctgaac gtgcccgccg
     1621 gtagcaccta cggcctgatt ggttctaacg gttcagggaa gtcgacgctg ctgaaactga
     1681 tggcgaacat ccatcggccg acgagtggca gcatcacgca ttccggccgg atctcggcgc
     1741 tactcgaact cggcgccggg ttccaccctg aactctccgg tcgcgacaac gtctacttga
     1801 acggttcgat ccttggcatg acccgcaagc aagtcgacgc cgccatggac gcgatcatcg
     1861 acttcagcgg gatcgaggag ttcatcgata ccccggtgaa ggtctactcg agtggcatgt
     1921 acgtgcggct cgggttttcg attgcggtca acctcgaccc agagatcctc atcatcgacg
     1981 agatcatcgc ggtcggcgac gaggagttcc aacggcgctg cttcgactac ctgtacgagc
     2041 ttcgcaagcg cggcgtcacc atcatcttcg tctcgcacag cttgccgctg gtgcagacct
     2101 tgtgcgaccg agcggcgtgg atcgagaagg gtgtgttgcg tgccgagggc tccgcactcg
     2161 aggtcgtcga tgcgtacgtc gcgaccgtca accgggccga gcgagtgcga ctggattccg
     2221 agggccacgg ccgggccgac ggcgatctcg agcggcgggg cggtggggag gctaccgtca
     2281 ccgccgtcac gttcatcggc tccgacggtg aaccgacgtt ggcggctact gcgggcgagc
     2341 ggctgacgct tcgcattgcc taccaggcaa gcaccgcact cgacgatgcc gtgttcgggc
     2401 tggcgttcca caccgagtcg ggagttctgg tgagcggccc caattccgaa ttcggcggca
     2461 ttcacagcgg ccggatccac ggcccgggcc acatcgacta caccgtggac tcgctcgcgt
     2521 tgacgccggc gacgtacctt gtttcggcgg ccattactga catcaccttg ctgcacgtgt
     2581 tcgactatcg cgaccgcgct tttaccctcg ccgtccagcc gggtaagtcg cccgaccgca
     2641 acggcgtggt gaccctcgac gggcactggg cgccgccgat ctcaggaacc ggaacggagt
     2701 tctcgcagtg agagtcaatc tcgggtgtgg ccaggcctac atggagggct ggacgaacgt
     2761 cgacgcgagc cccgacgtca aggctgacat ccaccttgac gccgcggagt tcgtgcgcca
     2821 atacggcgac caggtcgacg aggtctacat gggccatgtc ttcgagcacc tgatgcccgg
     2881 ctatgcggcg tcgttgctgc gcctgctcaa cgagcgcctg cgccccggcg ccgtcatctc
     2941 ggtggtcact cccgacatgc gcgcgatctt cgaggcctat ctcgctggtg agatcgacaa
     3001 cgacaagctc aacgcgtcgt tcatctactc ctacgtgcag ccgtcgcatc atgtgtggtg
     3061 ctacgacgag gcgacattgc ttgaactgtt ccgccgagcc gggttggccg acgccgagcc
     3121 gatcactgac gt
//
LOCUS       DATCBJ010000011         2827 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_256901, whole genome shotgun sequence.
ACCESSION   DATCBJ010000011 DATCBJ010000000
VERSION     DATCBJ010000011.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2827)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2827)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2827
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_256901"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..450)
                     /locus_tag="VII50_00200"
     CDS             complement(<1..450)
                     /locus_tag="VII50_00200"
                     /inference="COORDINATES: protein motif:HMM:NF017984.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0036297 - interstrand cross-link repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crosslink repair DNA glycosylase YcaQ family
                     protein"
                     /protein_id="HEY5049291.1"
                     /translation="MTGRAAVELAAVQLSADEARRFALAAQHLVGSPDRRAGVAGLVG
                     SLRAVQLDTISVLARSHELVAYARLGAVGRPKVEAAYWGGDAFEYWAHAACVMPISQW
                     PLFAFRRRHFQRRAKHWGQHSSSVAKAVLKRVRAEGPMTATDLGGAKK"
     gene            627..>2827
                     /gene="secA"
                     /locus_tag="VII50_00205"
     CDS             627..>2827
                     /gene="secA"
                     /locus_tag="VII50_00205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HEY5049292.1"
                     /translation="MPAIFDKLMRAGEGKILRQLKAVSDQVNSVEAEFEALSDAELRA
                     QTDEFKERYAAGESLDDLLPEAFATVREAARRTLGQRHFDVQIMGGAALHLGNIAEMR
                     TGEGKTLVSTLPVYLNALSGEGVHVVTTNDYLARRDSEWMGRVHRFLGLEVGVILSQM
                     PSAQRRTAYAADITYGTNNEFGFDYLRDNMALSAEELTQRGHYFAVVDEVDSILIDEA
                     RTPLIISGPVELNLKWYTDFASLVARLKRGEEGEGDYEADEKKRTVAISEQGVQRVED
                     WLGIENLYEATHTPLVGYLNNALKAKELFARDKDYVVIDGEVLIVDEFTGRMLYGRRY
                     NEGMHQAIEAKEGVEIKQENQTLATITLQNYFRLYQKLGGMTGTASTEAAEFDQIYKL
                     GVVPIPTNRDAVRIDQRDVVFKSEKAKFESVVDDIAERHEKGQPVLVGTISVEKSELL
                     SGMLLRRGVPHEVLNAKYHEKEAAIIAQAGRKGAVTVATNMAGRGTDIMLGGNPEFLA
                     QLELSEHGLSPVETAEEYEAAWPAAVAKWKGSVAAEHSEVVDLGGLYVLGTERHDSRR
                     IDNQLRGRSGRQGDPGESRFYLSLGDDLMRLFNGAAVEKIMDTLNIPDEVPIESKMVT
                     RAIRSAQTQVEQQNFEIRKNVLKYDEVLNKQRTVIYAERRRVLHGDDLHEQVRHMVDD
                     VIRGYVEAATAEGYAEEWNLEELWKALKTLYPVGVTIEEVETASGGGREGL"
BASE COUNT          545 a          918 c          937 g          427 t
ORIGIN      
        1 cttcttcgcc ccgccgaggt cggtggcggt catgggtcct tcggcgcgca cccgtttcag
       61 cacggctttc gctacggaac tcgagtgctg accccagtgc ttcgcgcggc gctggaagtg
      121 acgccgacga aacgcgaaca atggccactg cgagatgggc atcacgcagg cggcatgcgc
      181 ccagtactca aaagcgtcgc caccccaata cgccgcctcg accttgggtc gcccgaccgc
      241 gccgagcctc gcgtaggcaa cgagttcgtg cgagcgggcc agcaccgaga tcgtgtcgag
      301 ctggacggcg cgaagcgacc cgacgagccc cgcgaccccg gcccggcggt caggggaacc
      361 gacgaggtgt tgggcagcca gcgcgaatcg gcgggcctcg tcggctgaca actgcactgc
      421 ggccaattcc actgcggcgc gtccggtcat gggtcggatc gtaggtcgcg gcgctgacgc
      481 cggccgacac cagcgcacgc ccgccgccca cgcgacacgg gcgctacgcg cggtgatgcc
      541 tgcgcctcgc ctacgatggc tggaggacgg tgcgcacccc ccgccgtccc gctatcgtcc
      601 agcaccgttt cgaggagcca agcttcgtgc ccgcaatctt cgacaagctc atgcgcgccg
      661 gcgaggggaa gatccttcgc cagctcaagg cggtctccga tcaggtcaac tcggtcgaag
      721 cagagtttga ggccctctcg gacgccgagt tgcgggcgca gaccgatgag ttcaaggagc
      781 gttacgcggc cggtgagagc ctcgacgacc tgctgccgga ggcattcgcc acggtcaggg
      841 aagcggcccg acggacgctt gggcagcggc acttcgacgt ccagatcatg ggcggcgccg
      901 ccctgcacct ggggaacatc gcggagatgc gtaccggcga ggggaaaacc ctggtctcca
      961 cgctgccggt ctacctgaat gcgctctccg gcgagggtgt gcacgtcgtc accacgaacg
     1021 actacctggc ccgccgtgac tccgagtgga tgggccgggt gcaccgcttc ctcggcctcg
     1081 aggtcggggt gatcctgtcg cagatgccct cggcgcagcg gcgcaccgcc tacgccgccg
     1141 acatcaccta cggcacgaac aacgagtttg gcttcgacta cctgcgcgac aacatggcct
     1201 tgagcgccga ggagctcact cagcgtgggc actacttcgc cgtcgtcgac gaggtcgact
     1261 cgatcctgat cgacgaggcc cgcacgccgc tgatcatcag cggtccggtc gagctcaacc
     1321 tcaagtggta caccgacttc gcctcccttg tcgcccgctt gaagcgcggt gaagagggtg
     1381 agggcgacta cgaagccgat gagaagaagc gcaccgtcgc catcagcgag cagggcgtcc
     1441 agcgcgtcga ggactggctc ggcatcgaga acctttacga ggcgacgcac acgccgctgg
     1501 tcggctacct gaacaacgcg ttgaaggcca aggaactctt cgcacgggac aaggactacg
     1561 tcgtcatcga tggcgaggtg ctgatcgtcg acgagttcac cgggcgcatg ctctacggcc
     1621 gccgctacaa cgaggggatg caccaggcga tcgaggccaa agagggcgtc gagatcaagc
     1681 aggagaacca gactctcgcg acgatcacct tgcagaacta cttccggctg taccaaaagc
     1741 tcggcggcat gacaggtacc gcaagcaccg aggccgctga gttcgaccag atctacaagc
     1801 tcggcgtggt gccgatcccg acgaaccgcg acgccgtgcg aatcgaccag cgcgatgtgg
     1861 tcttcaagtc ggagaaggcc aagttcgagt cagtcgtcga cgacattgcc gagcgacacg
     1921 aaaagggtca gccagttctg gtcggcacca tcagtgtcga gaagtccgag ctgctgtccg
     1981 ggatgctgtt gcgccgcggc gtcccgcacg aggtgttgaa cgcgaagtac cacgagaagg
     2041 aagccgcgat catcgcgcag gccggccgca agggcgcggt cacagtggcc accaacatgg
     2101 ccggtcgagg caccgacatc atgctcggtg gcaaccccga attccttgcc cagcttgaac
     2161 tttccgagca cggtctgtcg ccggtcgaga cggccgagga gtatgaggcc gcgtggccgg
     2221 ccgctgtcgc taagtggaag ggctccgtcg ccgccgagca cagcgaggtc gtcgacctcg
     2281 gtggtctcta cgtgctcggc accgagcggc acgactcacg tcgcatcgac aaccagctgc
     2341 gcgggcggtc cggccgacag ggcgaccctg gagagtcgcg cttctacctc tcgctcggcg
     2401 acgacctgat gaggctgttc aacggcgctg cggtcgaaaa aatcatggac acgctgaaca
     2461 ttcccgacga ggtgcccatc gaatcgaaga tggtcactcg tgcgatccgt tcggcgcaga
     2521 cgcaggtcga gcagcagaac ttcgagatcc gcaagaacgt cctcaagtac gacgaggtgc
     2581 tgaacaagca gcgcaccgtc atctacgccg agcggcgccg cgttctgcac ggcgacgacc
     2641 tgcacgagca ggtgcgccac atggtcgacg acgtgatccg cggatacgtc gaggcagcaa
     2701 cggccgaggg ctacgccgag gaatggaacc tcgaggagtt gtggaaagcg ctcaagactt
     2761 tgtacccggt cggcgtcacg atcgaggagg tcgaaacggc gagcggcggt ggtcgcgaag
     2821 ggttgag
//
LOCUS       DATCBJ010000012         2865 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_403595, whole genome shotgun sequence.
ACCESSION   DATCBJ010000012 DATCBJ010000000
VERSION     DATCBJ010000012.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2865)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2865)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2865
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_403595"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..826
                     /locus_tag="VII50_00210"
     CDS             <1..826
                     /locus_tag="VII50_00210"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HEY5049293.1"
                     /translation="GLSGRDISLAGRIVALADSFEVMTAARAYKKAMTRGTALAEIVR
                     CSGTQFDPDVVRALLEVSAPRLRWAMGPTSWLMGTPLLGSAPSLTAAGVAAQAAVGVS
                     AVAVAGMAGVAGPALAANTAPPAVPNAQPGVATHAVAAPTDMATPPLARVPTKHSAPR
                     TIQLPTSASSASPTQITAATTVPAPSQTAALVRPATLTGPTDGHAATNPPAPKPTPTP
                     RATPAPAIAVPAVAAATKAAPPAPAPTPDATAKSTSGNHGNNGNRHGHGWGHGSGG"
     gene            complement(890..961)
                     /locus_tag="VII50_00215"
     tRNA            complement(890..961)
                     /locus_tag="VII50_00215"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(927..929),aa:Val,seq:cac)
     gene            1161..1601
                     /locus_tag="VII50_00220"
     CDS             1161..1601
                     /locus_tag="VII50_00220"
                     /inference="COORDINATES: protein motif:HMM:NF016564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsgA family sporulation/cell division regulator"
                     /protein_id="HEY5049294.1"
                     /translation="MKCMKRHQKRERVVEVRTDNVILDLEFSVVLADNDPVKVDAHLR
                     YDARDPFAVSVSFDAGVNQIEWTFARDLLDKGLWEAVGDGDVKVWPRAGAVFLALCSP
                     SGKAVLETRRPAIADFMARIERIVPVGRESEFVDVDRELEGLFS"
     gene            complement(1659..2675)
                     /locus_tag="VII50_00225"
     CDS             complement(1659..2675)
                     /locus_tag="VII50_00225"
                     /inference="COORDINATES: protein motif:HMM:NF012640.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I family
                     protein"
                     /protein_id="HEY5049295.1"
                     /translation="MTVDTRQAFAYVDGRLASGLVEVRHDLSALDERGFWVVVGGFDG
                     VVTCARFADVREAPLPDGAWSGPGRTEWTTSLDRAAYVEGVETIRALIEKGTVYQVNL
                     CRVLSARSDAGDLSGLASVLAAEHPSRYPALVCLPQAGIQVCSASPELFLSRSRVDGA
                     DVMATRPIKGTGRSSADISEKDRAENIMIVDMARNDLSRIASTGGVDVPALCELEEYP
                     GLVHLVSTVSATLVPRVTWTQIFAATFPPASVSGAPKSSALRIIRELEPVPRGPYCGA
                     IGWVDTDQQTACLAVAIRTFWLDAGQLRLGTGAGITWGSDPEAEWAETELKAELLLGL
                     ASRG"
     gene            complement(2672..>2865)
                     /locus_tag="VII50_00230"
     CDS             complement(2672..>2865)
                     /locus_tag="VII50_00230"
                     /inference="COORDINATES: protein motif:HMM:NF012703.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="arginase family protein"
                     /protein_id="HEY5049296.1"
                     /translation="LPGGLRAVELLAAAFMAGRDSNVRSVDIVEVDATADAPDFRTVR
                     LAALIVLEVAAGLALRPQA"
BASE COUNT          516 a         1014 c          914 g          421 t
ORIGIN      
        1 gggactcagt ggccgcgaca tctcgctcgc cggtcggatc gtcgcacttg ccgactcctt
       61 cgaggtgatg accgctgcga gggcttacaa gaaagcaatg acccgcggaa cagcgcttgc
      121 cgaaatcgta agatgcagcg gcacccagtt cgaccccgac gtggtgcgtg cgctgctcga
      181 agtgtccgca ccacgactgc gttgggcgat ggggcccacg tcctggctca tggggacacc
      241 gctgctcggc tctgcgccgt cactaaccgc ggcaggcgtc gctgcccaag ccgccgttgg
      301 cgtcagtgct gtcgcagttg ccggcatggc cggggtcgcc ggcccggcac tcgccgcaaa
      361 cactgccccg ccagccgtgc ccaacgcgca gccgggcgtg gctacccatg ccgtcgccgc
      421 gccaaccgat atggccacac caccactcgc acgcgtcccg acgaaacact cagcgccgcg
      481 aacaattcag ctacccactt cggcatcgtc ggcgagcccg acccagatca ctgcggcaac
      541 gacagtgccc gctcccagtc agacggctgc gttggttcgc ccggccacgc tcacagggcc
      601 caccgatggc cacgctgcga caaatccgcc ggcacccaaa cccacgccga ccccgagagc
      661 tacgccggca cccgccatag ctgtgcccgc cgtggccgcc gccaccaaag ccgcgccgcc
      721 agcaccggcg ccgacacccg acgcgaccgc gaaatccacc agcggcaacc acggcaacaa
      781 cgggaaccgg cacgggcacg gctggggtca cggttccggc ggctgactta ccggctgcgc
      841 ggccggcgtt tcgtcctgcg catggcagct gacctgtagg gacactgggt gggcgtacct
      901 ggtttcgaac cagggacctc tgccgtgtga aggcagcgct ctcccgctga gctatacgcc
      961 ccagcagcgc gtcactctac acgggcgacg cgcccggccc ggccggccaa aagtcgtcgg
     1021 gactggtggg tcgccacagg ttagaactgc gcgcaatcgg ggatccctgt ctcagtgctc
     1081 tccgcgaccc cgagatcaca cagagtaatg gtggtcactc agcgcaacct tgggtgccca
     1141 tcccaagatc gagaaccgcc atgaaatgca tgaagcggca ccagaagcga gaacgggtgg
     1201 tcgaagtgcg cacggacaat gtgattctgg acctggagtt cagtgtcgtg ctcgccgaca
     1261 acgatccggt caaggtggac gctcatttgc gctacgacgc gcgcgatccc ttcgcggtgt
     1321 ccgtgagctt tgacgccggg gttaatcaga tcgagtggac cttcgcccga gacctgctgg
     1381 acaagggcct atgggaagcc gtcggcgacg gcgacgtgaa ggtctggccc cgagccggcg
     1441 cggtgttctt ggctctctgc tccccctcag gcaaggccgt actggagact cgccggccgg
     1501 cgatcgccga cttcatggcg cggattgagc ggatcgtgcc agtcggccgc gagtcagaat
     1561 tcgtcgacgt cgaccgcgaa ctcgagggtc tcttttccta gcctcgcgtc accacgtgtg
     1621 cgcgatcgtc gcatcaccac atctgcgcga tcctcgcatc acccgcgcga cgccagcccc
     1681 agtagcagtt cggccttcaa ttccgtctcg gcccactccg cttccggatc cgagccccag
     1741 gtgatgcctg cacctgtccc caagcgcagc tgaccggcgt ccagccagaa cgttctgatc
     1801 gccaccgcga gacacgccgt ctgctgatct gtgtcgaccc acccgatcgc gccgcaatag
     1861 gggccgcggg gaaccggctc gagctcgcga atgatgcgta acgcactcga cttcggcgca
     1921 ccagagaccg aagccggcgg aaacgtcgcg gcaaagatct gcgtccaggt gacgcgggga
     1981 acgagcgtgg cggaaacggt cgagaccagg tgcacgagcc ctgggtactc ctcaagctca
     2041 cacagggccg ggacgtcgac accgccggtc gaagcgatgc ggctgaggtc gttgcgggcc
     2101 atgtcgacaa tcatgatgtt ctcggcgcgg tccttctccg agatgtccgc cgaggaacgg
     2161 ccggtgccct tgatcggtcg ggtcgccatg acgtccgcgc cgtcgacgcg actgcgagac
     2221 aagaacagct cgggactggc cgagcagacc tggatacctg cctgcggcag gcagaccagc
     2281 gcggggtaac gggacggatg ctcggcggcc agaaccgagg cgagcccgct cagatcgccg
     2341 gcgtcactgc gagcggacag cacccggcac aggttgacct ggtagaccgt gcccttctcg
     2401 atcagtgcac ggatggtctc gacgccttcg acataggcgg cgcgatcaag cgacgtcgtc
     2461 cactcggtgc gccccggacc cgaccacgcg ccgtccggca gcggcgcttc gcggacgtcg
     2521 gcgaaacgcg cgcaagtcac cacgccgtcg aagcctccga ccacgaccca aaaaccccgc
     2581 tcatcgaggg ccgaaaggtc gtgcctgacc tcgacgagtc cgctcgcgag ccggccgtcg
     2641 acatacgcga aggcctgcct ggtatcgacg gtcatgcctg gggtcgcagg gccagcccgg
     2701 cggccacttc gaggactatg agcgcagcga gccgcacggt gcggaaatcc ggggcatccg
     2761 cggtcgcatc gacctcgacg atgtcgaccg atcggacgtt ggagtcccga cccgccatga
     2821 acgcggccgc caggagttcg accgcccgca ggccgccggg caacg
//
LOCUS       DATCBJ010000013         6085 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_403538, whole genome shotgun sequence.
ACCESSION   DATCBJ010000013 DATCBJ010000000
VERSION     DATCBJ010000013.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 6085)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6085)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6085
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_403538"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..935
                     /locus_tag="VII50_00235"
     CDS             <1..935
                     /locus_tag="VII50_00235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008983 - protein-glutamate
                     O-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HEY5049297.1"
                     /translation="QAAIVIGPGKEYLVESRLLPLAREAGLDDVGALVARLQGSHDDG
                     LRRQIVEAMTTNETSFFRDRDPFNALSQVIVPELCKLRRGSHRLTLWSAACSTGQEPY
                     SIAMTLLDHALITADWHVDVLATDINGDVLERARRARYSQLEVNRGLPAPLLVRHFER
                     AETDWRVVAGVRKMVRFARLNLAEPFEVHQMDVVFLRNVLIYFDPATKIEVLRRVRAI
                     LRPDGFLFLGGAETTLGLDDGFERVAVGSTIAYRSKGAGPLDLPRRLAPAVAGIGLSG
                     TVPLGARPRPVAGAWQLSPQPPPPPQPVLRQPLN"
     gene            1070..1459
                     /locus_tag="VII50_00240"
     CDS             1070..1459
                     /locus_tag="VII50_00240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY5049298.1"
                     /translation="MEGARPMLAMVIDDSSAMRLILGRIMRSLGFDVVDAPNGAEAIA
                     RLQSGVMPDIALVDWNMPVMDGLAFVNAVRREEKWRQITLMMVTTESEQSQIVRALAA
                     GAHEYVIKPFTPDAIADKLALLGVLAY"
     gene            1481..1927
                     /locus_tag="VII50_00245"
     CDS             1481..1927
                     /locus_tag="VII50_00245"
                     /inference="COORDINATES: protein motif:HMM:NF025070.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheX"
                     /protein_id="HEY5049299.1"
                     /translation="MTYIPSADDLEVITREVWTSFLDGDPSGLIAAPGDMPADRITGC
                     VYLSGAYTGSIMLQCSPPAARDAAAALFSMAPAAVTQAEVVDALGEIANMVGGNVKSM
                     LPGPSTLSLPSVVQGQLSVPGARIVREVQLSWRDEPLFVSLWQRAS"
     gene            1935..2297
                     /locus_tag="VII50_00250"
     CDS             1935..2297
                     /locus_tag="VII50_00250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY5049300.1"
                     /translation="MRILIVDDSRAMRMIVARTLRMAGYDEHELVEAVDGLHALEVVA
                     QEPPDVVLCDWNMPLMSGLELLTALRSAGWRIPFGFVTSEASPQMRAAALEAGAMFLV
                     AKPFTPEDFQDALDPVLA"
     gene            complement(2324..4438)
                     /locus_tag="VII50_00255"
     CDS             complement(2324..4438)
                     /locus_tag="VII50_00255"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HEY5049301.1"
                     /translation="MTTTAPAVNTVPTIATANPAPGDVGPGRVKRLVRGRPADPVWVR
                     PSLFALLIGTALLYIWGLGASGWANSFYSAAVQAGSQSWKAFFFGSSDASNFITVDKP
                     PASLWVMELSARVFGVNAWSILVPQALEGVATVAVLYATVRRWFTPAAGLLAGAVLAL
                     TPVAVLMFRFNNPDALLVLLLTIAAYAVTRALEAASTRWLVLAGSLVGLGFLTKMMQA
                     FFVVPAFGLVYLLAAPTPVRRRIWQLLLAGVAMFVSAAWWVVAVTLTPASARPYVGGS
                     QHNSILELIFGYNGLGRLTGNETGSVTGGGGGNATGGAGGGSMWGPTGITRLFGSEMG
                     TQVSWLIPAALVMLLGALWLSRREGRTSKLRAATVLWGTTLIVSGLILSLSKGIIHPY
                     YTVALAPAIGALVGIGGTELWRHRRSLTSRLFLSAAIGVTTWWANVLLSRTPTFESWL
                     RPTILVAGIAAALTVLVIGHVKRGIAVAIAAGSMLTAVGAPVAFALDTASTPHTGSIP
                     TAGPSSGQGGPGGFGGARGGPTGGQRTGGFGTAPNGTAGGPPPTGTTGFAPPTGTAGA
                     TGTGNTGGFGGGRGQGGGGLLGSSTPSAALTAALKANASSYTWVAAAIGSNDASGYQL
                     ATGDPVMAIGGFNGSDPAPTLAQFETFVAAGKIHYFIAGSVGTPNGGSNASAQISTWV
                     SSHYTAVTIGGVRVYDLTKATS"
     gene            complement(4435..5688)
                     /locus_tag="VII50_00260"
     CDS             complement(4435..5688)
                     /locus_tag="VII50_00260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009081152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glycosyltransferase family 2/GtrA
                     family protein"
                     /protein_id="HEY5049302.1"
                     /translation="MTTTMQLPDELTEEQATSAPAIEIVIPVHNEEHDLEPSVRRLHE
                     YLTSTFPLSFRITIADNASTDDTWPIACALEAELANVRATYLSKKGRGLALHAVWLAS
                     AAEVVCYMDVDLSTDLSALLPLVAPLLSGHSDVAIGTRLARSSRVVRGPKREVISRCY
                     NVLLRTTLATQFSDAQCGFKAMRTDRARELLPMVQDNGWFFDTELLVLAERSGMRIHE
                     VPVDWIDDPDSRVDIVQTAIADGLGIARLARDLARGRIPLRAHRDEVSTGPLGAQLFR
                     FCSVGIASTLAYIALFLVARTTMSAAWSNAIALLITAIGNTAVNRSFTFGVRGRTARL
                     QHQLQGLAVFGIGLALSMGALAALHAAAPHAGRSIEVATLVVANLAATILRFLLFRSW
                     VFRRPSDALNSHPSRTSTTTVREFS"
     gene            complement(5798..>6085)
                     /locus_tag="VII50_00265"
     CDS             complement(5798..>6085)
                     /locus_tag="VII50_00265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5049303.1"
                     /translation="VEADAETAVARHRCMSCGDSKDVLDSAEHWNFPRMWACPSCSQS
                     IAELATGLHTTDDGAVSWIALAARCVECGTIDGLTDFTLDAVPADEVLNQL"
BASE COUNT         1077 a         1967 c         2010 g         1031 t
ORIGIN      
        1 gacaggccgc catcgtcatc ggcccgggca aggagtacct cgttgagagc aggctgctgc
       61 cgctggcccg cgaggccggc ctcgacgacg tcggggcgtt ggtcgcgcgc ctgcagggca
      121 gccacgacga tggcctgcgg cggcagatcg tcgaggcgat gaccaccaac gagacgtcgt
      181 tcttccgcga ccgggaccct ttcaatgcgc tgtcacaagt gatcgtgccc gaactgtgca
      241 agctgcgccg cgggagtcat cgtctgaccc tctggtcggc ggcgtgttcg accggtcagg
      301 agccgtacag catcgccatg acgctgctgg accatgcgct gatcaccgcg gactggcacg
      361 ttgacgtcct cgcgactgac atcaacggcg acgttctcga gcgagcccgt cgggccaggt
      421 attcgcagct cgaagtgaac cgggggctac cggcaccgct gctcgtccgg cacttcgagc
      481 gagcggagac cgactggcgg gtcgtggcgg gggttcgcaa aatggtccgg ttcgcccgcc
      541 tgaacttggc cgagcctttc gaggtacacc agatggacgt cgtgttcctt cgcaatgtcc
      601 tcatctactt cgatccagcc accaagatcg aggtgctgcg ccgggtccgc gcgatcctgc
      661 ggcccgacgg cttcctcttc ctgggcggcg ccgaaacgac gctcggactc gatgatggct
      721 tcgagcgggt cgccgttggt tccacgatcg cgtatcgctc caagggcgcc ggaccgctcg
      781 atctcccacg tcgccttgcg cccgccgtcg ccggtattgg cttgtccggg acggtccctc
      841 tcggcgcccg gccgcgaccc gtcgcagggg catggcagct gtcgccacag ccgcctccgc
      901 caccgcagcc cgtcttgcgc caaccgctca attagttggc gtaaaggccg atgaacaggc
      961 gagtactgat gaggcgaatg ccgcaacagc agccgccccg ctcgaccgat tagaacccta
     1021 gcaatacgaa gacccggctc cagttcagtc cggatcgacc gatttgacga tggaaggtgc
     1081 acgcccgatg ttggccatgg tgatcgacga ctcgagcgcg atgcggttga tccttggtcg
     1141 gatcatgcgg tcgctcgggt ttgacgtcgt tgacgccccc aatggtgctg aagccatcgc
     1201 gcggctgcaa agcggcgtca tgccggatat cgctcttgtc gactggaaca tgccggtgat
     1261 ggatggtctc gccttcgtga acgcggtccg tcgcgaagag aagtggcgtc agatcacgct
     1321 gatgatggtg acgaccgaaa gcgagcagtc gcagatcgtg cgcgcgctcg ccgcgggtgc
     1381 gcacgaatat gtcatcaagc cgttcactcc tgatgcgatc gctgacaagc tcgccctcct
     1441 cggcgtcctc gcctactagc gcgcaaagag gtagaaggcc gtgacttaca ttcccagtgc
     1501 cgatgacctc gaggtcatca cgcgtgaggt ctggacgtcg ttcctcgacg gtgacccatc
     1561 ggggctgatc gccgcgcccg gcgacatgcc cgcggacagg atcactggat gcgtctacct
     1621 gtcgggtgcc tacaccggat cgatcatgct ccagtgctcg ccgccggcgg cgcgcgacgc
     1681 tgctgcagcg ctgttctcca tggcgcctgc tgcggtcacg caagccgagg tcgtcgatgc
     1741 actcggcgaa atcgcgaaca tggtcggtgg caacgtcaag agcatgctgc ccggcccgtc
     1801 gaccctgtcg ctgccgtcgg tggtgcaagg tcagttgtcc gttcccggcg cgcggatcgt
     1861 tcgggaagtc cagctctcgt ggcgcgatga gccgctcttc gtgagcttgt ggcagcgggc
     1921 gtcgtaggtc cgcgatgcga atcctgatcg tcgacgacag cagagccatg cgcatgatcg
     1981 tggcgcgcac cctgcgaatg gctggctacg acgagcacga gctggtcgaa gcggttgacg
     2041 ggctgcatgc actcgaggtg gttgcgcagg agccaccgga cgtcgttctg tgcgactgga
     2101 acatgcccct gatgtcgggg ctggaactgc tgaccgcgct gcgatcggcg ggctggcgca
     2161 ttccctttgg cttcgtgacc tcggaagcct cgccccagat gcgggcagcc gcactcgaag
     2221 ccggcgcaat gttcctggtc gccaaaccct tcactcccga ggactttcag gacgcgctcg
     2281 acccggtcct cgcctagcgc ttgcgccggg ccgcggtgcg cggtcatgac gtggccttcg
     2341 tcaggtcgta gaccctgacg ccaccgatcg tcaccgcggt gtagtggctg ctcacccagg
     2401 tcgaaatctg ggccgacgca ttgctgccgc cgttcggagt gcccacgctg cccgcgatga
     2461 agtagtgaat cttgcccgca gcgacgaacg tctcgaactg cgccagcgtg ggcgccgggt
     2521 cgctgccgtt gaacccaccg atcgccatga cgggatcgcc cgtcgcgagc tggtatcccg
     2581 acgcgtcgtt ggacccgatc gcggcggcaa cccacgtgta tgaacttgcg ttcgccttca
     2641 gtgctgcggt cagtgccgcg ctcggtgtgc tgctgccaag cagcccgccg ccgccttgcc
     2701 cgcgaccgcc gccaaatcca cctgtgtttc cagtgccagt ggcgccggcc gtgccggttg
     2761 ggggtgcgaa gccggtggtg ccggtcggcg gtggaccacc tgccgtcccg ttcggtgctg
     2821 tgccgaaccc acctgtgcgc tggccgccgg tcgggccgcc acgagcgccg ccgaatccgc
     2881 cggggccacc ttggccggac gacggcccgg ccgtcgggat cgaaccggtg tgcggcgtcg
     2941 aggcggtgtc gagagcaaag gcaaccggtg cgccaactgc agtgagcatc gagcccgcag
     3001 cgatggcgac cgcgataccg cgctttacgt gcccgatgac cagcacggtg agggcggccg
     3061 cgataccagc gacgaggatc gtcggacgca gccatgactc gaaggtcggt gtgcggctaa
     3121 gcagcacgtt cgcccaccac gtcgtcaccc cgatcgcggc cgacaagaag agccgtgacg
     3181 tcaaggacct gcggtggcgc cacaattcgg tgccgccgat gcccacaagt gcaccgatcg
     3241 ccggcgcgag tgcgaccgtg tagtaggggt gaatgatccc cttgctcaag ctgaggatga
     3301 gtccgctcac gatcagcgtg gtgccccaca gcacagtcgc ggcgcgaagc ttgctggtgc
     3361 gtccctcgcg acggctcagc cacaaagcgc cgagtagcat gaccagcgcg gccgggatca
     3421 gccacgagac ctgggtgccc atctccgatc cgaacagtcg ggtgatgccg gtcggacccc
     3481 acatgctgcc gccaccggca ccgccggttg cgttgccgcc gcctcccccg gtgacgctgc
     3541 ccgtctcgtt gccggtcagg cggccgaggc cgttgtagcc gaagatcagc tcaagaatgc
     3601 tgttgtgctg cgatccgccg acgtaagggc gtgcgctggc cggcgtcaac gtgaccgcaa
     3661 cgacccacca cgcggctgag acgaacatcg caacaccggc aaggagtaac tgccagatcc
     3721 tgcgtcgaac gggtgtcggt gcagcgagca agtacacaag accgaatgcg gggacgacga
     3781 agaacgcctg catcatcttg gtcaggaagc caaggcccac caaagatccg gcaagtacca
     3841 gccagcgagt gctcgcggct tccagtgccc gagtcaccgc gtaggcagca atggtcagta
     3901 gcagaaccag caatgcgtct ggattgttga agcggaacat cagcacagcg acgggggtga
     3961 gggcgagcac agcgccggcg agcaaacccg ctgccggggt gaaccagcgt cgaaccgttg
     4021 catagagaac ggccaccgtg gccacacctt cgagtgcctg gggcaccagg atgctccagg
     4081 cgttcacacc gaagacgcgc gccgagagtt ccatgaccca cagtgacgct ggcggcttgt
     4141 cgaccgtgat gaagttcgac gcatcggagg agccgaagaa gaacgccttc cagctctgcg
     4201 agccggcttg cacggccgcc gagtagaacg agttcgccca acccgacgca ccgagacccc
     4261 agatgtagag gagcgccgtt ccgatcaaca aggcgaacag cgacgggcgc acccacacag
     4321 gatcggccgg tcggccgcgg accaggcgct tcacgcgccc cggcccgaca tcgcccggcg
     4381 cagggtttgc agtcgcgatg gtcggcactg tgttgacggc gggcgcggtg gtggtcatga
     4441 gaactccctg acggtcgtgg tgctggttcg cgacgggtgc gagttcagtg cgtctgacgg
     4501 cctgcggaag acccacgacc ggaacaacaa gaagcgaaga atggtggcgg cgagattggc
     4561 gactacgagc gtggcgactt cgatcgatcg ccctgcgtgc ggtgcggctg catgcagggc
     4621 cgccagggcc cccatgctca gcgcaaggcc gatgccgaag accgcgagcc cttgcagctg
     4681 gtgttgcaac ctggcggtac gacctcgcac accaaacgtg aaggagcggt tgacggccgt
     4741 gttgccgatc gctgtaatca gcagtgcgat cgcattcgac cacgcagcag acatggtcgt
     4801 tcgcgcgaca agaaagagtg cgatgtaggc gagcgtgctc gcgataccca cactgcagaa
     4861 gcggaacagc tgcgcgccta gcggcccagt gctgacctcg tcgcgatgcg ctcgcagtgg
     4921 tatccgtccg cgcgcgagat cgcgcgccag tcgggcgatg cccaggccgt cggcgatcgc
     4981 cgtctgcacg atgtcgacgc gggaatccgg atcgtcgatc cagtcgaccg gcacctcgtg
     5041 aatccgcatg cctgaccgct cggcgagaac gagtagttcg gtgtcgaaga accaaccgtt
     5101 gtcctgcacc atgggcaaca actcgcgtgc tcggtcggtg cgcatcgctt tgaatccaca
     5161 ttgcgcatcc gagaattggg tagcgagcgt cgtccgcagt agcacgttgt agcaacgcga
     5221 gatgacctca cgcttcggtc cacgaacgac gcgcgacgag cgagcaagcc gagtgccgat
     5281 cgcgacatcg ctatgccctg aaagcaaggg cgcaaccaac ggcagcagtg cggacagatc
     5341 ggtcgacaga tcgacgtcca tgtaacacac gacttccgcg gcgctcgcca accacacggc
     5401 atgtagggcg aggccacggc ccttcttgct gaggtaggtg gcacgcacgt tcgccagttc
     5461 ggcttccagt gcgcacgcga ttggccatgt gtcatcggtg ctggcgttgt cggcaatggt
     5521 gatccgaaac gacaagggga atgtcgatgt caagtattcg tgcaagcggc gaacgcttgg
     5581 ttcgagatcg tgttcttcgt tgtgcacggg aatgacgatc tcgatcgccg gggcgctggt
     5641 ggcctgctcc tcggtcagtt catccggcag ctgcatcgtt gtcgtcatga gatgacaatc
     5701 gcgtgccggc ctgcagcgaa cctgaactcg acctgacgac cgcgtcggag atttgtttcg
     5761 gagaagttgc gccctacagc cggcgcaagt gtgcgcctta cagctggttc aagacctcgt
     5821 ctgccggcac tgcgtcgaga gtgaagtcgg tcaggccatc gatggttccg cattcgacgc
     5881 agcgagcagc gagcgcgatc caactcactg cgccgtcatc ggtggtgtgc aagccggtcg
     5941 cgagctcggc gatcgactgc gagcagctcg ggcaggccca catgcgcggg aagttccagt
     6001 gttcggcgga gtcgagcacg tccttgctgt caccgcaact catgcagcga tggcgcgcga
     6061 ccgcggtctc cgcatcggct tcgac
//
LOCUS       DATCBJ010000014         8094 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_549945, whole genome shotgun sequence.
ACCESSION   DATCBJ010000014 DATCBJ010000000
VERSION     DATCBJ010000014.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 8094)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8094)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8094
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_549945"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..790
                     /locus_tag="VII50_00270"
     CDS             <1..790
                     /locus_tag="VII50_00270"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEY5049304.1"
                     /translation="AEAASAVVADINAAGGIASAHVGDCSAVDAATSLVNQAIHVFGR
                     LDAVVNNAGIVRDRTLAKMTAEEWDAVIAVHLRGHYALTHAACAYWKEHGGPGRIVCT
                     SSTSGLLGNFGQANYGAAKAGIAAFSTIVAQEMARYGVTCNAIAPAARTRMTEGAYGD
                     IAGDSGEFDFWHPDNVAPMVAFLCSSASANISGKVFGVQGDTVEIYRPWTSAGVIENG
                     ERRWDVDQLADRVMELFEESGIASGPENAMARLRFSMTDRTPRL"
     gene            925..1416
                     /locus_tag="VII50_00275"
     CDS             925..1416
                     /locus_tag="VII50_00275"
                     /inference="COORDINATES: protein motif:HMM:NF013179.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HEY5049305.1"
                     /translation="MADVGVAVVPGSKHDGMSYLLIPLIQRPSPELAPLRETLRAWVT
                     VVATSADPCIVLNREGRIAGLSAAAAALFAEAPVELVGRRLVGDVFTLVDFSSAAEPM
                     PDEAHVPPTQAIATGMLNRGLVRLRRSDGVTLTLDAIATPLHADDGTIIGSLTFVGQL
                     AAH"
     gene            complement(1381..2541)
                     /locus_tag="VII50_00280"
     CDS             complement(1381..2541)
                     /locus_tag="VII50_00280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5049306.1"
                     /translation="MAFVRILALIGGFAILGGTASSVLRTLVVPRGVKSKVSDMVLDS
                     TVRVSRLLADRIADYERRDNILAWAAPLSLMTLLFTWIASFWIGFSLLDFGFTHFTAS
                     AAMRQAGSSIATLGFAASPTASLTILDIVTGITGMVVVALQIAYLPTLYAAYNRRESL
                     ITVLSARGGEPAWGPEILARHVWLNLQGSLPDLYRDWETWAATVSESHTSYPVLIHFR
                     SPQPLRSWLIALLGVMDAAALQTALAPTTAPVEARLMLQMGYLTLRDLAAIESIPTRR
                     DVSPDDPIQLPYAEFEEAVDMLSSVGLQVERTAAQAWPHFRGWRINYEDAAYGLAQHI
                     DAVPARWSGPRRRQDQPIPPVRPINRQPTMSRDGEPTTTERSVGGELADEGQ"
     gene            2583..3209
                     /locus_tag="VII50_00285"
     CDS             2583..3209
                     /locus_tag="VII50_00285"
                     /inference="COORDINATES: protein motif:HMM:NF025935.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4446 family protein"
                     /protein_id="HEY5049307.1"
                     /translation="MSLSSSTAALLAVIGTSAGGVGLLVALFAHLRLRKLRRNLDILQ
                     AGDDAESFLSSAAKQVELVARLRAELSAVEGRMQRLRLDVADALRHVAVVRYDAFRDM
                     GGRMSFSAALLDDAGDGLVISAIHGRTETRSYAKGVKGGASDQQLSPEERQAIAFATR
                     TGPGPSGRQPTAAQKDEPAVLVRRANPAEVRLFDDDEIGLPAVDPIES"
     gene            3233..4174
                     /gene="pheA"
                     /locus_tag="VII50_00290"
     CDS             3233..4174
                     /gene="pheA"
                     /locus_tag="VII50_00290"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HEY5049308.1"
                     /translation="MPAVAPSRYAYLGPEGTFTEAALRTIPEAADAELLPYRSVSAAL
                     DAVRRGEVNAALVPLENSVGGGVASTIDELSTGELLHIQREVLLDVGFALAVRPGTPR
                     SSLKRVAAHPQAEAQCRRWIADNLSDVEIVTSASNAQAAASVASPASSLDGAITSALA
                     AQRYGLEVTDDDIHDHAGAVTRFVLVGQPTTPTAPTGTDKTSLVAFIGADHPGALLEI
                     LTEFAVRGINLTWIESRPTGVGLGRYCFSIDCEGHVVDARVSEALMGLRRICSDVRFL
                     GSYPRADGLPPTMRRGTSDTEFRDAAAWLAKVTAGEA"
     gene            4206..5468
                     /gene="serS"
                     /locus_tag="VII50_00295"
     CDS             4206..5468
                     /gene="serS"
                     /locus_tag="VII50_00295"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009085827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HEY5049309.1"
                     /translation="MIDIKLVRDDPDRVRTSQRMRGEDPALVDVLLAADERRRAAVLR
                     FDTVRASQKSLGKEVAAAKGPEKHALLAHAKDLAAEVREAEAELATADEALRVASLAL
                     SNVVEPGVPEGGEDDFALVATVGTVPSFDFTPRDHVELGKILGAIDVERAAKVSGSRF
                     YYLTGVGALLELALVNMAMMQAVAAGFTPMLPPALVRSRAMEGTGFLGQAAENVFRLE
                     ADDYYLVGTAEVPLAAYRMDETLALDELPLRYVGFSPCFRREAGTYGKDTKGIIRVHQ
                     FDKVEMFTFCAPEDAVAEHQRLLGWERQFLDALEIPYRVIDVASGDLGSSAARKYDCE
                     AWVPTQGKYREVTSTSNCTEFQARRLNIRLRDGDGVRSAATLNGTLVAIPRTIVAILE
                     NHQQADGSVVVPAALRPYLGRDVLEPVG"
     gene            5475..6290
                     /locus_tag="VII50_00300"
     CDS             5475..6290
                     /locus_tag="VII50_00300"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF019887.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEY5049310.1"
                     /translation="MSQQPRMVATDLDGTIVRSDGTISTRTVKALLTAEKTGATIVLV
                     TGRPPRWITTIAEQTDHHGIVICSNGAIVYDLRTEQILESTLIPVDTVAKVVGILSTA
                     LPDLGFAVENGFDVYRSPAYRGGWNIGPDVALPVHDLDVVTSHAAAKLLASHPEIAAD
                     ELLVIARGLVGDLVEPTHSNGRGLVEMGPLGVTKGTALARLAGERGIDRADVVAFGDM
                     PNDLPMLAWAGTGYAVANAHPDVLATVDLTTASNDDDGVAAVLEQLFAPRRAG"
     gene            complement(6311..6604)
                     /locus_tag="VII50_00305"
     CDS             complement(6311..6604)
                     /locus_tag="VII50_00305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5049311.1"
                     /translation="MAYGICSYLSGSTGNAFTIIVNNATGVFDFNGTVATAKQNQGTD
                     TSTAIPGLGDKAAGVGQQIVVQSGGKTIDIRNVDETPWTQSIALATLVIASLH"
     gene            complement(6952..7437)
                     /locus_tag="VII50_00310"
     CDS             complement(6952..7437)
                     /locus_tag="VII50_00310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterial proteasome activator family protein"
                     /protein_id="HEY5049312.1"
                     /translation="MADDRTPEDPQVEIIDPDDDASGGTVADLVNQPAKVMRIGNMIR
                     QLLEEVRSAPLDEAGRVRLKEIHAASVKELESGLSPELVDELERITLPFSESEIPSES
                     ELRIAQAQLVGWLEGLFHGIQTALVAQQMAAHAQLEQMRRALPPGTPPEGQRPNQGMY
                     L"
     gene            complement(7449..>8094)
                     /locus_tag="VII50_00315"
     CDS             complement(7449..>8094)
                     /locus_tag="VII50_00315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HEY5049313.1"
                     /translation="AGVPEAACTVWSTVFQAAAARAGETLLVHGGTSGIGTFAIQLAR
                     ALGVRVLTTAGTPEKCARAVALGAELAIDYRRDDFVSAIRSHTGGAGVDVILDVVGGE
                     YLARNLEALAPDGRLVVLAMQRGRRAELDLGMLMAKRASVYSAGLRARPLSQKAAIVA
                     AVRSAVWPLVESGAIRVVVQAEVPMAQAAEAHRLMEAGDHVGKILLTIAETIAV"
BASE COUNT         1310 a         2653 c         2735 g         1396 t
ORIGIN      
        1 cgctgaggcc gcgtccgcgg tggtcgccga catcaacgct gcgggcggca tcgccagcgc
       61 gcatgtgggc gactgcagtg cggtcgacgc agcgacatcc cttgtcaatc aggcgattca
      121 cgtcttcggc cggctcgacg cggtggtgaa caacgcgggc atcgtgcgcg atcgcacgct
      181 ggccaagatg actgccgagg aatgggacgc ggtcatcgcg gtccacctgc gagggcatta
      241 cgcgctgacc cacgcggcgt gcgcgtattg gaaggagcac ggtggcccgg gccgcatcgt
      301 atgtacgtcg agcacctcag ggctcctagg caacttcggc caggcgaact acggcgccgc
      361 gaaagctggc atcgctgcct tctcgacgat cgtcgcccag gagatggcgc gctacggggt
      421 gacctgtaac gcaatcgcgc ctgcggcgcg gacccggatg accgaaggcg cctacggtga
      481 tatcgccggc gatagcgggg aattcgactt ctggcacccc gacaatgtcg cgccgatggt
      541 ggcattcctt tgcagttcgg cgtctgcgaa tatcagcggc aaggttttcg gagtacaggg
      601 cgacacagtg gagatatacc ggccatggac gtcggccggc gtcatcgaga acggcgaacg
      661 gcgatgggac gtcgatcagc tggcggaccg ggtcatggag ctgttcgagg aatcaggaat
      721 tgcgagcggc ccggaaaatg ccatggcgcg gctacgtttc tccatgacgg accggacgcc
      781 tcgtttgtag catcctgcgg ctatttgcgc tgcatgcgct gcgccgaatg ggctcgctcg
      841 gtctgtacct acgcacggtg tcgatgctgc ttcgcctgtg tcacgctgat gagcagcaaa
      901 taactggggg atgtggataa tcgggtggct gacgttgggg tagccgtcgt tcctggctcc
      961 aaacacgatg gcatgtcgta cctgttgatt ccgctgatcc agcggccgtc accagaactt
     1021 gctccgctgc gcgaaacatt gcgcgcgtgg gtcacggtcg tggcaacgtc ggccgacccg
     1081 tgcatcgtgt tgaaccgtga gggccggatt gccgggctgt ctgccgcggc cgcggcgttg
     1141 ttcgcggagg cgcccgtcga gctggtcgga cgtcgattgg ttggcgacgt gttcaccctc
     1201 gtcgacttca gcagcgccgc ggagcccatg cccgacgaag cgcacgtgcc gcccacgcag
     1261 gcgattgcta ccgggatgtt gaaccgcggc ctggtccgac tgcgtaggtc ggacggcgtg
     1321 acgctgacgc tcgatgcgat cgcgacgccg ctgcacgcgg acgacggcac gattatcggc
     1381 tcactgacct tcgtcggcca actcgccgcc cactgatcgt tcggtcgttg tcggctcgcc
     1441 gtctcgcgac attgtcggct gccgattgat tggccggacc ggcgggatgg gctggtcttg
     1501 gcgacgacgg ggacctgacc atcgcgcggg taccgcatcg atgtgctgtg cgagcccgta
     1561 ggccgcgtcc tcgtagttga tccgccaacc acgaaaatgc ggccatgctt gcgctgcggt
     1621 gcgctcgacc tgcaagccga ctgagctcag catgtcgacg gcttcctcga attccgcgta
     1681 aggcagctga atcgggtcgt ccggcgagac atcgcggcgg gtcggaatgg actcgatcgc
     1741 ggcgagatcg cgcaacgtca ggtatcccat ctggagcatc agtcgggcct ccaccggcgc
     1801 ggtcgtcgga gcgagcgcag tttgcaacgc cgcggcgtcc atgactccga gtagggcgat
     1861 caaccacgag cgaagaggtt gcggcgatct gaagtggatc aggaccgggt agctggtgtg
     1921 actctccgac accgtcgccg cccaggtttc ccagtcccgg tagagatccg ggaggctccc
     1981 ctgcagattc agccacacat gccgggcaag gatttccgga ccccacgcgg gttcgccgcc
     2041 gcgcgccgag agcacggtga tcagcgactc gcgccggttg tacgccgcat agagggtcgg
     2101 caagtaggcg atctgcaggg cgacgaccac catgccggtg atcccggtca cgatgtcgag
     2161 gatggtcagc gatgcagtcg gggatgccgc gaacccgagc gtcgcgatcg acgagcccgc
     2221 ctggcgcatc gccgcgctgg cggtgaagtg ggtgaacccg aagtcgagca ggctgaaacc
     2281 gatccagaac gaggcgatcc aggtgaacag cagcgtcatc aacgacagcg gtgccgccca
     2341 cgccaggatg ttgtcgcgcc gttcgtagtc ggcgatgcgg tcggcgagca ggcgagacac
     2401 ccgtacggtc gagtcgagca ccatgtcgga aaccttcgac ttcacgccgc gtgggacgac
     2461 gagagtccgc agcacgctcg atgcggtgcc gcccaggatg gcgaatccgc cgatgagcgc
     2521 gaggatccgg acgaatgcca tggccggacg atactggctg cggtgcggtt aggctgagcg
     2581 ccgtgtcgct ctccagttcg actgctgcct tacttgccgt gatcgggacg tcggccggtg
     2641 gcgtcggcct gctggtcgca ctgttcgccc atctgcggtt gcgcaagctc cgccgcaacc
     2701 tcgacatcct gcaggccggc gacgatgccg agtcgttctt gtcctcggcc gcgaaacagg
     2761 tcgagctggt cgcccggttg cgtgccgagc tgtcggctgt cgagggtcgc atgcaaaggc
     2821 ttcgactcga cgtggccgac gcgctgcgcc atgtcgcggt cgttcgctac gacgcctttc
     2881 gggacatggg cggccgcatg tcgttctcgg ctgcgctact cgatgacgcc ggcgacgggt
     2941 tggtgatcag cgcgattcac ggccgaacag aaacgcgctc gtacgcgaag ggcgtcaagg
     3001 gcggcgctag cgaccagcag ctctcgccgg aggagcggca ggcaattgcc tttgccacca
     3061 ggaccggtcc gggtccgagc ggacgccagc ccaccgccgc ccaaaaggat gagcctgcgg
     3121 tccttgtccg gcgggctaac cccgccgagg tccggttgtt tgacgacgac gagatcggac
     3181 tcccggccgt cgacccgatc gagtcctgaa gccgccacga taaagtcggc gcatgcccgc
     3241 agttgcgccc tcccgctatg cctacctggg ccccgagggc acgttcaccg aagcggcgtt
     3301 gcgcacgatc ccggaagctg ccgacgcgga gttgctgccg tatcgcagcg tctcggctgc
     3361 gcttgacgcg gtccggcgcg gtgaggtcaa cgccgcgttg gtgcccctgg agaactcggt
     3421 tggcggcgga gtggcctcga ccatcgacga gttgtcgacc ggcgagctgc tgcacattca
     3481 gcgtgaggtg ctgctcgatg tcgggttcgc ccttgccgtc cggcctggaa cgccgcgctc
     3541 ctcgctgaag agagtggccg cgcacccgca ggccgaagcg caatgccggc gttggatcgc
     3601 cgacaactta agcgacgtcg agatcgtgac gtcggcgtcc aatgcgcagg cagcggcatc
     3661 tgtcgcttcg cctgcgtcga gcctcgatgg agccatcacc tcggccttgg cggcgcagcg
     3721 ctacggactc gaggtcaccg acgacgacat ccacgatcat gcgggtgcag tgacgcgttt
     3781 cgtgctcgtc gggcagccca ccacgccaac tgccccgacc ggcaccgaca agacctcgct
     3841 cgtggcgttc atcggtgcgg accacccggg cgcgctgctc gagatcctca ccgagttcgc
     3901 cgtgcgcggc atcaacttga cctggatcga gtcccggccc acgggtgtcg ggctcggccg
     3961 gtactgcttc tcgatcgact gcgaaggtca cgtcgttgac gccagggtga gcgaggcgtt
     4021 gatgggactg cggcgcatct gcagcgacgt ccgcttcctt ggcagttacc cacgcgccga
     4081 cgggctgccg ccgaccatga ggcgggggac ctcggacacc gagttccgcg acgcggcggc
     4141 ctggctggcc aaggtgacgg ccggcgaagc ctgaccaccc aacccccctc ggatagcctc
     4201 gtgcggtgat cgacatcaag ttggtgcgtg acgaccccga ccgggtgcgc acgtctcagc
     4261 ggatgcgtgg cgaggatccg gcgctcgtcg acgtgctact cgcggccgat gaacggcgcc
     4321 gtgccgccgt gctgcgcttc gacaccgtgc gtgcgtcaca gaaatcgctg ggcaaagagg
     4381 tcgccgctgc gaagggtccc gaaaagcatg cgttgcttgc gcacgcgaag gatctcgcgg
     4441 ctgaggttcg cgaggccgag gccgagctcg ctacggctga cgaagcgctg cgcgttgcgt
     4501 cactggcgtt gtcgaacgtc gtcgagcccg gggtccctga aggtggcgag gacgatttcg
     4561 cgctggtcgc caccgtcggc accgtgccgt ccttcgactt caccccccgc gatcacgtcg
     4621 agctcggcaa gatcctcggt gcgatcgacg tcgagcgcgc tgccaaggtc tccggttcac
     4681 ggttctacta cctgaccggc gtgggggcgt tgctcgagct cgcactcgtc aacatggcca
     4741 tgatgcaagc ggtcgccgcc ggcttcaccc cgatgttgcc gccggcgctc gtgcggtcgc
     4801 gggcgatgga gggaaccggg ttcctcggtc aggccgcaga aaacgtgttt cgcctcgaag
     4861 ccgatgacta ttacctcgtc gggacggcgg aggtgccgct cgctgcgtac cgcatggacg
     4921 agacgttggc cctggatgaa ttgccgttgc gctacgtcgg attctcaccg tgctttcggc
     4981 gtgaggccgg tacctacggc aaggacacca agggcatcat tcgcgttcat cagttcgaca
     5041 aggtcgagat gttcacgttc tgcgcccccg aggacgccgt cgccgaacac cagcggctgc
     5101 tcgggtggga gcggcagttt ctcgatgccc tcgaaatccc gtaccgggtg atcgatgtcg
     5161 cgtccggtga cctcggctca agtgcggcgc gcaagtacga ctgcgaggcg tgggtgccga
     5221 cgcagggcaa gtatcgcgag gtcacctcga cgtcgaactg cacggagttc caggcgcggc
     5281 gactgaacat ccggctgcgc gatggcgacg gcgtccgatc cgccgcgacc ctcaacggaa
     5341 cgctggtcgc gatcccccgc acgatcgttg cgatcctgga gaaccatcag caggcagacg
     5401 gctctgttgt ggtgccggcg gccctgcggc cctacctcgg gcgcgacgtg ctcgaaccgg
     5461 ttgggtaggc cgcggtgtcg cagcaaccgc gaatggtcgc gaccgatctg gacggcacca
     5521 tcgtcaggtc ggacggcacc atttcgaccc ggacggtgaa ggcgctgctc acggccgaga
     5581 agaccggcgc cacaatcgtc ttggtgacgg gccggccgcc gcgctggatc accacgatcg
     5641 ctgagcagac agatcaccac ggcatcgtga tctgctccaa cggcgccatc gtctacgacc
     5701 tgcgcactga acaaattctc gagtcgacgc tgatccccgt cgacaccgtg gccaaggttg
     5761 tcggcatcct ctcgaccgcg ctgcccgacc tcggatttgc ggtggagaac ggcttcgacg
     5821 tctaccgatc gccggcgtac cggggtggct ggaacatcgg ccccgacgtc gcgttgccgg
     5881 ttcacgatct cgacgtggtg acctcccacg ccgccgcgaa gttgctggcc tcgcatcccg
     5941 agatcgctgc ggatgagctc ctggttatcg cgcgcggctt ggtcggcgac ctggtcgagc
     6001 ccacccattc gaacgggcgc ggcttggtcg agatgggacc gctcggggtc accaaaggca
     6061 ctgcgctggc gcgtctagct ggggagcgcg gcatcgaccg cgccgacgtg gtcgccttcg
     6121 gcgacatgcc caacgatctg ccgatgctcg cgtgggccgg tacgggctat gcggtggcga
     6181 atgcgcaccc cgacgtgctc gcgaccgtcg acctgacaac cgcaagcaac gacgacgacg
     6241 gcgtcgcagc ggtcctcgag cagctcttcg ccccgcgccg tgcgggttga tcacgtaagg
     6301 gggcgatcgg tcagtgcaag ctggcgatca ccagcgtggc gagcgcaatc gactgggtcc
     6361 agggcgtttc gtcgacgttg cggatgtcga tggtcttgcc gcctgactgg acgacgatct
     6421 gctgcccgac gccggcggcc ttgtcaccga gccccggaat ggcggtcgac gtgtcggtcc
     6481 cctgattctg cttcgcggtc gcgacagtcc cgttgaagtc gaagactccg gtggcgttgt
     6541 tcacaatgat ggtgaacgcg ttgcccgtgc tgccgctcag gtaagaacaa atgccgtagg
     6601 ccactgatcg gatccgcgta ggtcacgccg atcgcggtgg tgatctgagc ccagtcagcg
     6661 ggcaaccagc agccgacgca gatccgccgg cggccgcggg tgcgctggca ccgcccgcag
     6721 cgcctgagtt ggctgccgcg gctaccgacc ctgccgctgc agcggccgtg gatgcggtaa
     6781 ccgtgtcggc cgagatcgcc ttctcgaact gttgcatccg gcgacaaact cggtgacggc
     6841 gagggggctg caatcgtcag cttgttggtt gctggcaggt cgactccttg ttaggtgtcg
     6901 ccagccaaac agccgcccgt aacggctcag tgattcgtgg agatgtagtg cctagaggta
     6961 catgccctgg ttcgggcgtt ggccttcagg cggtgttcct ggcggcaaag cgcgccgcat
     7021 ctgctccagc tgagcgtgcg ccgccatctg ctgcgccacg agtgcggtct ggatcccgtg
     7081 aaagaggccc tcgagccagc cgaccagctg cgcctgcgcg atacgcagct ccgattcgct
     7141 cgggatctcg gactcgctga acggcagcgt gatgcgctcg agctcatcga ccagctcagg
     7201 tgacagaccg ctctcgagct ccttgacgga cgccgcgtgg atctccttca gccgcacccg
     7261 gccagcttca tcgagcggtg ccgaccgtac ctcttcaagg agctgacgga tcatgttgcc
     7321 gatccgcatc accttggccg gctggttcac caaatccgcg accgtgccgc cggacgcatc
     7381 gtcgtccgga tcgatgatct cgacctgtgg atcctcgggc gttcgatcgt ctgccatgcc
     7441 accgatactc atacggcgat tgtctcagca atcgtgagca ggatcttgcc cacgtggtcg
     7501 cccgcttcca tcaggcggtg cgcctcggcg gcttgcgcca tcggcacttc cgcctgcacg
     7561 acgacccgga tcgcgcccga ttcgacgagc ggccagacag ccgaccgaac cgcggcaacg
     7621 atggccgcct tctgagaaag gggccgcgcg cgcaaccccg cggagtacac cgacgcgcgc
     7681 ttggccatga gcattccgag atcgagttcc gctcgtcgtc cgcgttgcat ggcgagcacc
     7741 acgagtcgac cgtccggtgc gagtgcctcc agattgcgcg ccaagtactc cccgccgaca
     7801 acgtcgagga tcacgtcgac ccctgcgccg ccggtatggg agcggattgc cgacacgaag
     7861 tcgtcgcgcc ggtagtcgat cgccagctcg gcacccagcg ccaccgcccg cgcgcacttc
     7921 tccggcgtcc cggcggtggt gagaacgcgg acgccgaggg cgcgggcaag ctgaattgcg
     7981 aacgtaccga tgccgctcgt tccgccgtgc acgagcagcg tctcgccggc gcgtgcagcc
     8041 gcggcctgaa agacggtcga ccacaccgtg caggccgcct cgggcacccc ggcc
//
LOCUS       DATCBJ010000015         4866 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_476487, whole genome shotgun sequence.
ACCESSION   DATCBJ010000015 DATCBJ010000000
VERSION     DATCBJ010000015.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 4866)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4866)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4866
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_476487"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..907)
                     /locus_tag="VII50_00320"
     CDS             complement(<1..907)
                     /locus_tag="VII50_00320"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HEY5049314.1"
                     /translation="MTRHLLSAGDLSRDDALLILDTATELARLSDRPIKKLPTLRGRT
                     VVNLFFEDSTRTRSSFEAAAKRLSADVINFSAKGSSVSKGESLKDTALTLEAMGADGV
                     VIRHEASGAPHRLAGWIKGSVVNAGDGTHEHPTQALLDAFTMRRHLGNLEGRRVAIVG
                     DVLHSRVARSNVLLLHTLGADVTVVAPPTLFPVGIDGWSCSYSYDLDAVLPKTDVVMM
                     LRVQRERMNASYFPNAREYSRRYGLDAARMAVLPDHAIVMHPGPMNRGMEIAAEVADS
                     ERSVIVEQVTNGVSVRMAVLYLLLGG"
     gene            complement(904..1590)
                     /gene="pyrR"
                     /locus_tag="VII50_00325"
     CDS             complement(904..1590)
                     /gene="pyrR"
                     /locus_tag="VII50_00325"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HEY5049315.1"
                     /translation="MTAAHPHQRQNDQHDELRRGDQPDQQVPSAMPREVLEAPDMSRA
                     LTRIAHEILERTRGGADVLLLGIPTRGVGLARRLAERMSAVENRAVPVGSLDITMYRD
                     DLRMRPPRPLARTEVPGDGVDGMLVVLVDDVLFSGRTVRAALDALGELGRPRAVQLAV
                     LIDRGHRELPIKADYVGKNLPTSVREQVTVLIEELDGRDAVLLGPTSASGSASVAGSA
                     SVTAQGGDAR"
     gene            1874..2365
                     /locus_tag="VII50_00330"
     CDS             1874..2365
                     /locus_tag="VII50_00330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEY5049316.1"
                     /translation="MNDYAKALGARLRAIRTQQGLSLHGVEEKSRGRWKAVVVGSYER
                     GDRAVTVQRLAELAEFYGVPVGELLPDAAPSGASEPPPRLVIDLEQLADVPPEKARPL
                     ARYAATIQSQRGDYNGRVLSIRQDDLRSLAVIYDESPSLLTEELISWGVLNAEARRAI
                     DTL"
     gene            complement(2426..3772)
                     /locus_tag="VII50_00335"
     CDS             complement(2426..3772)
                     /locus_tag="VII50_00335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009074580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HEY5049317.1"
                     /translation="MDPTNELRAELPRGQLITDGDAVEAYRRDEARLVEPGHPLGVVL
                     ARAVGDVQATMRWATRHRVPVVARGAGTGLSGGATAIDGCVVLSLAKMNAIVEVNAGD
                     QLAVVEPGVINVDLQTAAAEYGLMFAPDPASFEISTIGGNIATNAGGLRCVKYGVTRD
                     SVLGLEVVLADGRIMKPGRRTVKGVAGYDLTALFVGSEGTLGIVTQATVKLRPRPSTD
                     PVTVVGVFATLTSAGEAVDDVMGAGLQPSLLELMDNATIRAVDDWKHVGLDRTAAAML
                     IAQVDSEHPREPALAIEKAFAAAGATYTAVSTDATEAAELLGIRRMTHWAIERLGMIL
                     TEDVGVPRSKLAQMLARIEEIGAQYEVVIATVGHAGDGNLHPSLVVDSERAQPAAEAI
                     YTAALALGGTITGEHGIGVLKRDWLPAEIGEVGMDVQRAVKATFDPLGILNPGKAI"
     gene            3853..4095
                     /locus_tag="VII50_00340"
     CDS             3853..4095
                     /locus_tag="VII50_00340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5049318.1"
                     /translation="MTATDAARGFSALLTAVERDGETFLVTRGGRTIARIEPARGTSG
                     AAVKALLRHYPADREWQSELAAIKASLNTQERSWQD"
     gene            4083..4475
                     /locus_tag="VII50_00345"
     CDS             4083..4475
                     /locus_tag="VII50_00345"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HEY5049319.1"
                     /translation="MARLILDSEVLIATERAGKHLNGVIDDDDDVAIAAISAAELLTG
                     VNLADATDRDKRTAFVRSVLDTIPIEVYDLAVARAHADLLAHVRRVGRPRGAHDLIIA
                     ATAVATDRIVVSADSRAYADLPGVSVRS"
     gene            complement(4494..>4866)
                     /gene="nusB"
                     /locus_tag="VII50_00350"
     CDS             complement(4494..>4866)
                     /gene="nusB"
                     /locus_tag="VII50_00350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HEY5049320.1"
                     /translation="VLFECDLRSSDALQTLARHISTATPPVPAYTVTLVEGVVAHRPR
                     IDELIETYAVGWTLDRMPVVDRNLLRIGTFELLWAEEVPDGVVLSEAVKLARDLSTDD
                     SPAFVNGVLAKLLDEKPALSV"
BASE COUNT          854 a         1642 c         1578 g          792 t
ORIGIN      
        1 tgccgccgag caagaggtac agaaccgcca tccgtacgct cacaccgttc gtcacctgct
       61 cgacgatgac cgagcgttcg gagtcggcga cctccgccgc aatttccatg ccccggttca
      121 ttggtcctgg atgcatgacg atcgcgtggt cgggcagtac tgccatgcgc gcggcatcga
      181 gaccatagcg acggctgtac tcgcgcgcat tcggaaaata cgacgcgttc atccgctcgc
      241 gttgaacgcg caacatcatg acgacatcgg tcttgggcag cactgcatcg aggtcgtagg
      301 agtacgagca ggaccaccca tcaatgccga ctgggaacaa ggtcggcggt gcgacgactg
      361 tgacgtccgc accgagcgtg tgcagtagca ggacgttcga gcgagccacc ctgctgtgca
      421 aaacatcgcc gacgatcgcg acccggcgtc cttcgagatt gccgaggtgg cgacgcatcg
      481 tgaaggcatc aagaagggcc tgcgtcgggt gctcgtgggt gccgtcgcca gcgttcacga
      541 cgctgccctt gatccaaccg gccaatcggt ggggagcgcc gctggcttcg tgccggatga
      601 ctacgccgtc ggcgcccatc gcctcgagcg tcagcgcggt gtccttcagg ctctcgccct
      661 tcgacacgct tgaccccttc gccgaaaagt tgatcacgtc ggcggacagc cgtttcgcgg
      721 cagcctcgaa cgagctgcga gtgcgggtgg agtcttcgaa gaagaggttg acgaccgtgc
      781 ggccgcgaag ggtgggcaac ttcttgattg gccggtccga tagacgggcg agttcggtcg
      841 cggtgtcgag aatcagcagc gcgtcgtcgc ggctcagatc gccggccgag agtaggtgcc
      901 gagtcatcgt gcatcaccgc cctgtgctgt tacggaagca gagcctgcaa cggacgcaga
      961 ccctgaagcg gaagtggggc ctagcaacac ggcgtcccgc ccgtcgagct cttcgatgag
     1021 caccgtcacc tgctcgcgca ccgacgtcgg caggttcttg cccacgtagt cggctttgat
     1081 tggcagctcc cgatgtccgc ggtcgatgag cacggccagc tgcaccgccc gcggtcgccc
     1141 gagctcgccc agtgcgtcga gcgcggcgcg gacggtgcgc ccggagaaca agacgtcgtc
     1201 gacgagcacg acgagcatcc cgtcgacacc gtcgccgggc acctcggtgc gggcgagcgg
     1261 gcgaggcggc cgcatccgca ggtcatcgcg gtacatcgtg atgtcgagcg agccgaccgg
     1321 gaccgcccgg ttctcgacgg cgctcatccg ctccgccagc cgacgcgcga gccccacgcc
     1381 gcgggtcgga atgccgagaa gcaggacgtc ggcgccacca cgggtacgtt cgaggatttc
     1441 gtgagcgata cgtgtcagcg cgcggctcat gtcgggtgct tcgagcacct cgcgaggcat
     1501 cgcggacggt acttgctggt cgggctgatc gccccggcgc aactcgtcgt gctggtcgtt
     1561 ctggcgctgg tgcggatggg cagccgtcat cgccgggacc tccttccccg cctcaccgga
     1621 cgggccttaa aggatgtcga gcagatctca aacagcgcca acctagcaga cctgcttgac
     1681 gcagagtgac gatgggccta ccgtcacagt gcgtaacgag cgcggctttg acttcgggat
     1741 ttctgggtca ctatgtcccc attcaggggc atcccccgaa gtagccactg gtgaaaccgg
     1801 cgcccgggag tgaccaggcg tgagcctggt ccggccagtt cactcacccg atcgcgccac
     1861 cgatgaggtc accttgaacg actacgcgaa ggcgctcggc gcccgattgc gcgccatccg
     1921 cacccagcag gggctgtcct tgcacggcgt cgaggagaag tcgcgcggcc gctggaaggc
     1981 cgtcgtcgtc ggctcctacg agcgtggcga ccgggcagtg accgtccagc ggctggccga
     2041 gctcgctgag ttctacgggg tcccggtcgg tgagttgctg cccgacgcag caccaagcgg
     2101 tgcgagcgag ccgccaccgc gcctggtcat cgacctcgag caactggccg acgtcccgcc
     2161 cgaaaaggcg cgcccgctgg ctcgctacgc agcgactatc cagagccagc gtggtgacta
     2221 caacggacgc gtgctctcga ttcggcagga cgatctgcgc tccctcgcgg tcatctacga
     2281 cgagtcgccg agcctgctga ccgaagagct gatctcgtgg ggcgtcttga acgccgaagc
     2341 acgccgggcc atcgacaccc tgtaatccct cgcagcccgc actgcacatg cagcgggtgc
     2401 acaagcgaca cccagggctg cgaagctaga tggctttgcc tgggttgaga atgccgagtg
     2461 ggtcgaaggt cgccttcacg gcgcgctgca cgtccatccc gacctcgccg atctccgccg
     2521 gcagccagtc gcgtttcaat acgccgatgc cgtgctctcc ggtgatcgtt ccgccgagcg
     2581 caagggcggc ggtgtagatc gcctctgcgg ccggctgtgc acgctcagag tcgaccacca
     2641 ggctcggatg caggttcccg tcaccggcgt gcccaacggt ggcgatgacg acctcgtact
     2701 gcgccccgat ttcctcgatg cgggcgagca tctgcgccag cttcgatcgg gggacgccga
     2761 catcctcggt gagaatcatt ccgagccgtt cgatcgccca atgcgtcatt cgccggatgc
     2821 cgagaagctc cgcggcctcg gtcgcatcgg tcgatacggc ggtgtaggtc gcgccagctg
     2881 cggcgaatgc cttctcgatc gcgagcgcgg gctcgcgcgg gtgctcggag tcgacctgcg
     2941 caatcagcat cgcggctgcg gtccggtcga gaccgacgtg cttccagtcg tcgacggctc
     3001 ggatcgtcgc gttgtccatc agctcaagaa gactcggttg cagtccggca cccatgacgt
     3061 catcgaccgc ttcgcccgcg gacgtcagag tcgcgaagac gccgacgacc gtgactgggt
     3121 cggtactcgg ccggggtcgt agcttcacgg tggcctgggt cacgatgccg agcgtgccct
     3181 cggacccaac gaacagggcg gtgaggtcgt aaccggcaac gcccttcaca gtgcgccgcc
     3241 cgggcttcat gatccggccg tcggcgagca ccacctcaag gccgagcacg gagtcgcgag
     3301 taacgccgta cttgacacac cgcaaaccac cggcgttcgt cgcgatattg ccgccgatgg
     3361 tcgagatctc gaagctggcc ggatcgggag caaacatcaa gccatactcg gcggcggcgg
     3421 tttgcagatc gacattgata acgcccggct cgaccaccgc aagttggtca cccgcattga
     3481 cctcgacgat ggcattcatc ttcgcgagcg agagcacgac gcagccgtca atcgcagtcg
     3541 cgccgccgga caacccagtg cccgcacccc tcgcgaccac gggaacccgg tggcgggtcg
     3601 cccaccgcat cgtcgcctgc acatcgccga cggcacgagc gaggacgacg ccgagcgggt
     3661 gtccgggttc gacgagccgc gcttcgtcgc ggcggtaagc ctccacggcg tcgccgtcgg
     3721 tgatcagttg cccccgtggg agttccgcac gcaactcatt ggtcggatcc acggcgttca
     3781 gactagcgtc cgacccggcg gggagcagtc gacctcacaa cggaaatcag ataatctgac
     3841 gacatgagag aggtgaccgc caccgacgcc gcacgcggct tctcggccct gctcaccgcc
     3901 gtggaacggg acggcgagac cttcctggtc acccgcggcg gccggacaat cgcgcgcatc
     3961 gagcccgcac gcggaacgtc cggcgctgcg gtgaaggcgc tccttcgcca ctaccccgcc
     4021 gatcgcgaat ggcaaagcga actagctgca atcaaggcgt cgctcaacac gcaggagcgc
     4081 tcgtggcaag actgatcctc gacagcgaag tcttgatcgc caccgaacgc gccggcaagc
     4141 acctcaacgg ggtgatcgat gacgacgacg acgtggccat cgcggccatt agcgcagcgg
     4201 aacttcttac gggggtcaac ctcgcggacg ctactgaccg cgacaaacgg actgctttcg
     4261 tccgcagtgt cctcgacacc atccccatcg aggtctacga cttggccgtc gctcgcgcgc
     4321 atgccgactt gctcgcccat gtgcgccgcg tcgggcgccc gcgcggcgca catgatctga
     4381 tcattgcggc aacggccgtc gccaccgatc ggatcgtcgt ttccgctgac agcagggcat
     4441 acgccgacct acctggcgtg agcgttcgca gctgaaccga cgagtaggac tgttcagacg
     4501 gacagcgcgg gcttctcgtc gagcaacttc gcaagcaccc cgttgacgaa cgccggcgag
     4561 tcgtcggtcg acaggtcacg ggccagcttg acggcctcgc tgagcacgac gccgtcaggt
     4621 acctcctcgg cccacagcaa ctcgaacgtg ccgatccgca acagattgcg atccactacc
     4681 ggcatccggt caagcgtcca gccgacggcg tacgtctcga tcagctcatc gatccgcgga
     4741 cggtgtgcga ccacgccttc gaccagtgtg acggtgtacg caggcacggg tggggtggcc
     4801 gtcgagatgt gccgggccag ggtctggagt gcatctgagc tacgcaggtc gcactcgaac
     4861 agcacg
//
LOCUS       DATCBJ010000016         2087 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_109717, whole genome shotgun sequence.
ACCESSION   DATCBJ010000016 DATCBJ010000000
VERSION     DATCBJ010000016.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2087)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2087)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2087
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_109717"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..540
                     /locus_tag="VII50_00355"
     CDS             <1..540
                     /locus_tag="VII50_00355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HEY5049321.1"
                     /translation="EGSGFVFADGRVMTNAHVVAGVHNPQVQVGGVGPQLAATVVYFD
                     PKVDVAILVVKDLHAKPLTFSGVLGSSADAVVAGFPEDGPYQTVAARIRGAELARGPD
                     IYQDADVTRDIYAVRAQVQPGNSGGPLLDTSGSVAGVVFGRAVNDSSTGYALTASQVS
                     AAAIAGRTATAPVSTHGCD"
     gene            complement(537..1001)
                     /locus_tag="VII50_00360"
     CDS             complement(537..1001)
                     /locus_tag="VII50_00360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009083138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF309 domain-containing protein"
                     /protein_id="HEY5049322.1"
                     /translation="MSIERDRDPSGRARNARPRDGLGRPLPRGSGDGSERVPDELHMS
                     VDDTVAFAQRYLDSGRPFHAHEVLEALWKTQPAGERDLWQGLAQIAVGLTHAMRGNTV
                     GATTLLRRGAGRVADYRGDTHGIDIDVVLTWADALAAAPGVPPPPLALRADR"
     gene            complement(1012..1944)
                     /locus_tag="VII50_00365"
     CDS             complement(1012..1944)
                     /locus_tag="VII50_00365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY5049323.1"
                     /translation="MPVTDSSVVMVDGPWTHREVSANGMRFHAAEMGAANQDPLVVLL
                     HGFPQFWWSMRHQLQDIAAAGYWVIAPDLRGYGGSDKPPRGYDAFTLASDIAGIVRSL
                     GARDAIIVGHDWGGYGAWCTAVLHPGAVRGIVTLAAPHPLRFRSALLTEPRGQFMASA
                     HLVGYQRPWVPERQLVGDDADAVGRMLHAWSAPSWPDEESERRYREQIQIPHVAHSAL
                     EYYRWTLRSQLRRDGQRFNRQLRQPVGVPTLQLHGALDTCVLPRTAAGSGRYVGGPYT
                     WRLIEDAGHFLQEESPKAVSAEILHWLDELDHSR"
BASE COUNT          341 a          665 c          731 g          350 t
ORIGIN      
        1 gaaggcagcg ggttcgtctt cgccgacggt cgggtcatga cgaacgccca tgtggttgcc
       61 ggcgtgcaca atccgcaagt acaggtcggc ggcgtcggcc cgcagttggc ggccacggtc
      121 gtgtacttcg acccgaaggt cgacgtggcg atcctggtgg tgaaagacct gcatgccaag
      181 ccgttgacct tcagcggagt cctcggatcg tcagccgacg cggtagtcgc cggctttcca
      241 gaagacgggc cataccagac ggtggctgcg cgcatccggg gtgctgaact cgcgcgcggg
      301 ccggacatct accaggacgc cgacgtgacc cgcgacatct acgccgtccg cgcccaggtt
      361 cagccgggca actccggcgg acccttgctc gacacgtccg gcagcgttgc gggagttgtg
      421 ttcggcagag cggtcaacga ctcgagcacg gggtatgcgt tgaccgcgtc acaggtgagt
      481 gcggcagcaa tcgcagggcg gacggcgacg gcacccgtct ccacgcacgg ctgcgactag
      541 cggtccgcgc ggagagccaa tggtggtggc gggacgccgg gtgctgctgc gagggcgtcg
      601 gcccaggtga gcacgacgtc gatgtcgatg ccgtgcgtgt cgccgcggta gtcggcgact
      661 cttccagcac ctcggcgcag taacgttgtc gcaccgacgg tgttcccgcg cattgcgtgg
      721 gtgagaccga ccgcgatctg cgcgagtcct tgccacagat cgcgctcgcc ggccggctgc
      781 gtcttccaga gggcctcgag aacctcgtgc gcgtggaacg gacgaccgct gtcgagatag
      841 cgctgggcga aagcaacggt gtcgtcgacg ctcatgtgca gttcgtcggg cacccgctcg
      901 ctgccgtcac ccgacccgcg cggcagcggt cggccgagcc cgtcgcgcgg cctggcattg
      961 cgcgcgcgac ctgatggatc gcggtcacgc tcgattgaca tggccggtcg atcagcggct
     1021 gtggtcgagt tcgtccagcc agtgcaggat ttctgcggac acggccttcg gtgattcctc
     1081 ctgcagaaag tgcccggcgt cctcgatcaa tcgccaggta taagggccac cgacgtagcg
     1141 acccgaacct gccgccgtcc gcggcagtac gcaggtgtcg agagcaccgt gcaattgcaa
     1201 cgtcgggacg ccgacgggct ggcgtagttg tcggttgaac cgttgcccgt ctcgtctcaa
     1261 ctgcgagcgc agcgtccagc ggtagtactc gagggcggag tgggcgacat gcgggatctg
     1321 gatctgctcg cggtagcgcc gctcggattc ttcatcaggc caactcggcg ccgaccatgc
     1381 gtgcaacatg cgccccacgg cgtccgcgtc atctcctacg agctgccgct cgggaaccca
     1441 cggtctttgg tatccgacga ggtgagcgct ggccatgaac tggccgcgcg gctcagtgag
     1501 cagggcgctg cgaaagcgca gtggatgcgg cgcagcaagg gtgacgatgc cgcggaccgc
     1561 gcccggatgc agcaccgcag tgcaccatgc gccgtagcca ccccagtcgt gcccgacaat
     1621 gatcgcgtcg cgggcgccga gtgatcggac gatcccggcg atgtccgatg cgagggtgaa
     1681 ggcgtcgtag ccacgcggtg gtttgtcact gccgccgtag ccacgcaggt caggggcgat
     1741 cacccagtag ccggcggcag cgatgtcctg gagctgatgg cgcatcgacc accagaactg
     1801 cggaaagccg tgcagcagaa cgacgagcgg gtcttggttg gccgcgccca tctcggcggc
     1861 gtggaagcgc attccattcg cgctgacctc gcggtgggtc cacggtccgt cgaccatcac
     1921 cacggaggag tccgttaccg gcatggcgcc cagcgtgcca caggttgggg cagatgatgc
     1981 tgcgtgattt tcgtgaggca cctgggctgg ctcgccgacc ggtggccaaa ttagtgacgg
     2041 ttcttcacct cccaggtgaa aaagcgtcac taattctggg atcgggg
//
LOCUS       DATCBJ010000017         2280 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_329567, whole genome shotgun sequence.
ACCESSION   DATCBJ010000017 DATCBJ010000000
VERSION     DATCBJ010000017.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2280)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2280)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2280
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_329567"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            297..857
                     /locus_tag="VII50_00370"
     CDS             297..857
                     /locus_tag="VII50_00370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5049324.1"
                     /translation="MRRSAIIVVNVVCGALVTSACSSGSANPGVAQLPATGSTPAVSG
                     APASSGAPKGAASSLAHAECMRSHGVPDFPDPGPLRIDVRTHPDLDPSSPPFVAAQKA
                     CLSLAPGGTAGGTVSAQQQAAALAYSSCMRSHGFPKFPDPVFSDGGEHVTINGINMGS
                     AQFQNADKVCQTSTGLGGLPSGGSGP"
     gene            854..1960
                     /locus_tag="VII50_00375"
     CDS             854..1960
                     /locus_tag="VII50_00375"
                     /inference="COORDINATES: protein motif:HMM:NF024923.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-binding protein"
                     /protein_id="HEY5049325.1"
                     /translation="MKATVGGIGLVVAASVGLGFVLTSAESNAVQPAAGQPAVASGPA
                     ATSSSPSPTAQPAPAASAIPFVASSGIAVQLNFSSPGVLTAVMVKVGDHVTKDQVLAT
                     VDSTAARIAVTSAQANADKAKTALAVLTQGLSAQELAQLNLGEAQGATAITTAQQALA
                     DAQAQADQDAATQNSLLSAAEQSLTDSEAQASQDATDSAQQVAVAQAQLSSDEAQAHP
                     DPVKIAADTAAVAAAQNSQADTALRDTQQVHAATANVTNAQNNVSATALHDSQAIHQG
                     QANVTTAQQQLAATKVADAVRTEPPRSTALASARDDVTAAQAQLQSAELALSATSLVA
                     PANGVVAAINAVVGESVAAGGATEFITLEEPGSS"
     gene            1957..>2280
                     /locus_tag="VII50_00380"
     CDS             1957..>2280
                     /locus_tag="VII50_00380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5049326.1"
                     /translation="MTSERGWISRRVLVTVVVVVVVIGGVIGGVALFGTKAPAKAASV
                     DNAAPTSTATVAQRSLSAQTNVSATLGYAGSYSVVNQAAGSFSQLPTVGEVIEPGEVL
                     YAVGGQ"
BASE COUNT          409 a          722 c          781 g          368 t
ORIGIN      
        1 ggcggatcag gtgggccgtg atgccggccc agggtgacaa cgaggtgaca agtcgtgtca
       61 cctcgccgtc accggcgccg ccgtacaaca agaagaccat cgatccgagg agcaggagga
      121 cgagcatcga catctgcccg aactcacccg gcgctcactc aaggacgagc ttgaccgcct
      181 acccgtataa cggttatata ggtggtctga ccgttaagtt cggaagggac aagccgcgca
      241 tggacccccg ttttgctgac cgacatcgat ccactccagc gcggcgatgc tcactggtgc
      301 ggcggtccgc catcatcgtg gtgaacgtgg tgtgtggcgc actcgtcacc tccgcctgta
      361 gcagcggctc ggccaaccct ggtgtcgcgc agctcccggc caccggctcg acccccgcag
      421 tgtcgggcgc gccggccagt tccggagcgc ccaaaggtgc ggcgtcgtcg ctcgctcatg
      481 cggaatgcat gcggtcgcac ggcgtgcccg acttccccga tcccggccca ctgcgaatcg
      541 atgtccgcac ccaccccgac ctcgatccca gctcgccgcc gtttgtggcg gcgcaaaagg
      601 cgtgcctgtc gttggcaccg ggcggcactg cgggcggcac tgtcagtgct cagcagcagg
      661 cagcagcgtt ggcgtactcc tcctgtatgc gatcgcacgg ttttccgaag ttccccgacc
      721 cggttttcag cgacggtggt gagcacgtca cgatcaacgg gatcaacatg ggctcggcac
      781 agtttcaaaa cgctgacaag gtgtgccaga cgtcgacagg gctaggtggc ctcccgtcgg
      841 ggggcagtgg cccgtgaagg ccaccgtggg tggcatcggt ctcgtagtcg cggcaagtgt
      901 cgggctcggc ttcgtgttga cgagtgccga aagcaacgcg gtgcagcccg ctgcgggaca
      961 gcccgcggtc gcgagtggac cggcggcgac ctcatcgtcg ccgtcaccta cggcacaacc
     1021 cgctcctgcg gcgtccgcta ttccgttcgt tgcaagttct ggcatcgccg tccaactgaa
     1081 tttctcgtcg cctggggtgc tgactgcggt gatggtgaag gtcggcgatc acgtcaccaa
     1141 ggatcaggtc ttggctaccg tcgattcgac cgccgcacgg attgcggtca ccagtgcgca
     1201 ggcgaacgcc gacaaggcga agactgcgtt ggccgtgttg acgcaggggt tgtcggcgca
     1261 ggagctggct cagctcaacc ttggtgaggc acaaggagca acggcgatta ctaccgcgca
     1321 gcaggcgttg gcggacgcgc aggcccaagc tgatcaggac gccgcaacgc agaacagtct
     1381 gctcagcgca gcggaacagt cgctgaccga cagcgaggcg caggccagtc aggatgcgac
     1441 ggattcggcg cagcaggttg ctgttgcgca ggcgcagctg tcttccgatg aggcgcaggc
     1501 gcatcccgat ccggtgaaga ttgctgccga tacggcggca gttgcagcag cccagaacag
     1561 ccaggcggac accgcgctgc gggacaccca gcaagtgcac gcagcgacgg ccaatgtgac
     1621 caacgcccag aacaatgtgt cggcgacggc gttgcacgac tcacaggcga tccaccaggg
     1681 gcaggcgaat gtgaccactg cgcagcagca actcgccgcc acgaaggtgg ccgatgcggt
     1741 gcgcacggaa ccgccgaggt ccactgcgct tgctagcgcc cgcgacgatg tgacggccgc
     1801 gcaagcccaa ctccaatctg ctgagctcgc cctgagcgcg acgtcgcttg ttgcgcccgc
     1861 gaacggagtc gtcgcggcga tcaacgcggt ggtcggggag tcggtggcgg ccggtggcgc
     1921 gaccgagttc atcaccctcg aggagcctgg cagttcatga caagtgaacg gggatggatc
     1981 tcgcggcggg tcctggtgac cgtggtggtt gtggtcgtcg tgatcggcgg ggtgatcggc
     2041 ggtgtggcgc ttttcggaac gaaggcgccg gcaaaagctg cgagcgtcga caatgcagcg
     2101 ccgacatcga ccgctaccgt cgcgcagcgg tcgttgtcgg cgcagacgaa cgtcagcgca
     2161 acgctgggct acgcgggcag ctacagcgtc gtcaatcaag cggcgggaag cttcagccaa
     2221 ctaccaacgg tcggcgaggt gatcgagccc ggcgaggtgc tgtatgcggt gggcggccag
//
LOCUS       DATCBJ010000018         2162 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_144129, whole genome shotgun sequence.
ACCESSION   DATCBJ010000018 DATCBJ010000000
VERSION     DATCBJ010000018.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2162)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2162)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2162
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_144129"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(69..1688)
                     /gene="groL"
                     /locus_tag="VII50_00385"
     CDS             complement(69..1688)
                     /gene="groL"
                     /locus_tag="VII50_00385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020643281.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HEY5049327.1"
                     /translation="MPKILEFDEDARRSLERGVDKLADTVKVTLGPRGRNVVIDKKWG
                     APTITNDGVTVAKEIELENPYENLGAQLAKEVATKTNDVAGDGTTTATVLAQAMVHLG
                     LRSVAAGANPMDLKRGIDLAAAEVSERLLKSAREVEEQTEIAHVATISAQDVKIGELI
                     AEAFAKVGKDGVVTVEESPTMGLELEFTEGLQFDKGYVSQYFVTDQERLEAVLEDAYV
                     LVHQNKISSMADFLPLLEKVAQTKKPLLVIAEDVEGEALATIVVNKIRGLLNIVAVKA
                     PGFGDRRKAMLGDIATLTGATLVTEDLGIKLESVGLDALGSARRIVVTKDNTTIVDGA
                     GDQTAIQERIRQIQTEIEATDSDWDKEKLQERLAKLSGGVCVLRAGAATEVELKEKKH
                     RLEDAISATRAAIEEGIIVGGGAALVHVASGLDDLGKTGDVATGVLVVKKALLEPARW
                     IALNAGQEGSVVVAKIAGLPAGHGYNAATGEYGDLMSQGVIDPVKVTRSAVQNAASIA
                     GMLLTTETLIVEKPAEETSESADGHSHGHSH"
     gene            complement(1803..2114)
                     /gene="groES"
                     /locus_tag="VII50_00390"
     CDS             complement(1803..2114)
                     /gene="groES"
                     /locus_tag="VII50_00390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003966899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HEY5049328.1"
                     /translation="MVTTATKVVIKPLEDRIVVKPLEAEQVTASGLVIPDTAKEKPQE
                     GKVLAVGPGRFDDKGARVPVDVKVDDVVLYSKYGGTEVKYSGEEYLVLSARDVLAIIE
                     K"
BASE COUNT          289 a          733 c          738 g          402 t
ORIGIN      
        1 cgcgggggcc acggtgatcc gtggcccccg cgttcgcggg gtgaccctgt gcggcgcgtc
       61 tcgcgtgatt agtgcgagtg cccgtgcgag tggccgtcgg ccgactcgga ggtctcctca
      121 gccggcttct cgacgatcag ggtctcggtc gtcagcagca tgccggcgat ggacgcggcg
      181 ttctgcacgg cggaccgggt gaccttaacc gggtcgatga cgccctggct catcaggtcg
      241 ccgtactcgc cggtggcggc gttgtagccg tgaccggcgg gcagcccggc gatcttggcg
      301 accaccacgg agccctcctg gcccgcgttg agcgcgatcc agcgggccgg ctccaacagc
      361 gccttcttga cgacgagcac gcccgtcgcc acgtcgccgg tcttgccgag gtcatcgagg
      421 ccggacgcga cgtgcacgag tgccgccccg ccgccgacga tgatgccctc ttcgatcgcc
      481 gcgcgggtcg ccgagatcgc gtcctcaagg cggtgcttct tctccttgag ctcgacctcg
      541 gtggccgcgc cggcccggag cacgcagacc ccgccggaga gcttggccag ccgctcctgc
      601 agcttctcct tgtcccagtc cgagtccgtg gcctcgatct cggtctggat ctggcggatc
      661 cgctcctgga tcgcggtctg gtcgccggcg ccgtccacga tcgtggtgtt gtccttggtc
      721 acgacgatgc gccgggcgga gccgagcgcg tcgaggccaa ccgactccag cttgatgccg
      781 aggtcctcgg tgacgagggt cgcgccggtc agcgtggcga tgtcgcccag catggccttg
      841 cgacggtcgc cgaagcccgg cgccttcacc gcgacgatgt tgagcaggcc gcggatcttg
      901 ttgacgacga tcgtggcgag cgcctcgccc tccacgtcct cggcgatcac cagcagcggc
      961 ttcttggtct gggcgacctt ctcgagcagc ggcaggaagt cggccatgga ggagatcttg
     1021 ttctggtgga cgagaacgta agcgtcctcg agcaccgcct caagacgctc ctggtcggtg
     1081 acgaagtact gcgagacgta gcccttgtcg aactgcaggc cctcggtgaa ctcgagctcg
     1141 aggcccatcg tcggggactc ctcgacggtg accacgccgt ccttgccgac cttggcgaac
     1201 gcctcggcga tgagctcgcc gatcttgacg tcctgggcgg agatggtcgc gacgtgcgcg
     1261 atctccgtct gctcctccac ctcgcgggcg gacttgagca gccgctccga cacctcggcg
     1321 gcggcgaggt cgatgccgcg cttgaggtcc atcgggttgg cgccggccgc gaccgagcgg
     1381 agcccgaggt ggaccatcgc ctgggcgagc acggtcgcgg tggtggtgcc gtcaccggcg
     1441 acgtcgttcg tcttggtggc gacttccttg gcgagctgag cgccaaggtt ctcgtagggg
     1501 ttctccagct cgatctcctt ggcgacggtc acgccgtcgt tggtgatcgt gggagcgccc
     1561 cacttcttgt cgatgacgac gttgcggccg cgcgggccga gcgttacctt cacggtgtcg
     1621 gcgagcttgt ccacgccacg ctcaagggag cgtcgggcgt cctcgtcgaa ctccaggatc
     1681 ttgggcattg acttacctca acctacggtt ggatcaggtg cggtacttgg acctgggtgt
     1741 cgccgacggg cccccggccg cccgatgtga cgggcagccg ggcgcacgcg cgggcgcttg
     1801 atctacttct cgatgatcgc gagcacgtcg cgggccgaga gcaccaggta ctcctcgccc
     1861 gagtacttca cttcggtgcc gccgtacttg ctgtacagca ccacgtcgtc gaccttgacg
     1921 tcgacgggga cgcgagcgcc cttgtcgtcg aaccgacccg gaccgaccgc gaggaccttg
     1981 ccctcctggg gcttctcctt cgcggtgtcc gggatgacca gcccggacgc agtgacctgc
     2041 tcagcctcga gcggcttgac cacgatgcgg tcttcgagcg gcttgattac aaccttggtg
     2101 gcggtcgtca cgatctgacc tccccctccg gaaacgtatt ggaaagttgg cttgcgatgg
     2161 cg
//
LOCUS       DATCBJ010000019         2324 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_33432, whole genome shotgun sequence.
ACCESSION   DATCBJ010000019 DATCBJ010000000
VERSION     DATCBJ010000019.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2324)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2324)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2324
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_33432"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(9..1139)
                     /locus_tag="VII50_00395"
     CDS             complement(9..1139)
                     /locus_tag="VII50_00395"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase family protein"
                     /protein_id="HEY5049329.1"
                     /translation="MEPQQEQQPGDRKLRVAVLFGGRSTEHSISCITAGSVLAALDPL
                     RYDVVPIGITREGRWVLASDDPATYQIQGGALPQVAASDASVVLAADPTAPELVVSEP
                     GAVPRQLVDVDVVLPLLHGPYGEDGTIQGLLELAGIPYVGAGVLSSAVAMDKHYMKLV
                     LQGGGLPDIPHVVVRPREWDEDPSAVRESVASLGFPVFVKPARGGSSIGISKVHDVGE
                     LDDAIELAQRSDPKVIVEAMVEGREIEVGVLEGIDGGAPEVSLAAEITVRDREFYDFD
                     AKYLALDTDIAVPASLTADVEAQIRRLAVMAFEALSCEGLARVDFFVTSAQRVLVNEV
                     NTMPGFTPTSVFPRVWAASGLDYPALLDRLIQTALRRPTGLR"
     gene            complement(1150..2157)
                     /locus_tag="VII50_00400"
     CDS             complement(1150..2157)
                     /locus_tag="VII50_00400"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046167 - glycerol-3-phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HEY5049330.1"
                     /translation="MTKAAVFGTGSWGTAFGMVLSDAGTETVLCGRRLEIVESINSQH
                     HNPWYQEGVELPDTLRATLDPAEALASADLVVLAIPAQTLRANLAVWMPLFPADALLV
                     SLIKGVELGTTKRMSEVICEVADAPAQRVAIVTGPNLAREIAERQPGASVVACTDADN
                     AERLQLACQTAYFRPYTNTDVVGCELAGAVKNVIALAVGIADGMGFGDNAQASLITRG
                     LAETARLGVALGADPHTFSGLAGLGDLVATCSSPLSRNRTVGEHLGRGMSVEAALAAT
                     TTTAEGVMSSKSILELAHKHDIEVPIIEVVVSVLYEQLQPGDGVARLMGRTAKAERHT
                     Q"
BASE COUNT          410 a          782 c          749 g          383 t
ORIGIN      
        1 ctgcgccgtc agcgcagacc ggttggtcgc cgcaacgcgg tctgaatcaa ccggtcgagc
       61 aacgccgggt agtcaagacc gctcgccgcc cagacccgtg ggaagactga ggtcggggta
      121 aagccgggca tggtgttgac ctcgttgacg agcacccgct gtgccgacgt cacgaagaag
      181 tcgaccctgg caagcccctc gcacgacaat gcctcgaacg ccatcacggc aagtcgccgg
      241 atctgcgcct cgacgtccgc ggtcagcgat gccggaaccg cgatgtcggt gtcaagcgcg
      301 aggtatttcg catcgaagtc atagaactcg cggtcgcgca cagtgatctc ggcggcgaga
      361 ctgacctctg gcgcaccgcc gtcgatgcct tccaggacgc cgacctcgat ctcacggccc
      421 tcgaccatcg cttcgacgat cactttcggg tcgctgcgct gcgccagctc gatggcgtcg
      481 tcgagttcgc cgacatcgtg caccttgctg atgccgatac tcgacccacc ccgggccggc
      541 ttcacaaaga ccggaaatcc caagctggca accgattcac gcacggccga cggatcctcg
      601 tcccactcgc gcggccgcac caccacgtgc gggatgtcgg gcaggccgcc gccctgcagc
      661 acgagcttca tgtagtgctt gtccattgcc accgccgacg aaagcacgcc ggcaccgacg
      721 tacggaatcc ccgcaagctc gagcaatcct tgaatggtgc cgtcctcgcc atagggtccg
      781 tgcagcagcg gcaacacgac gtcgacatcg acaagttggc gtgggacggc gcccggttcg
      841 gacaccacaa gctcgggcgc agtcgggtcg gcggcgagca cgactgaggc gtcagacgcc
      901 gcaacctgcg gtaacgcgcc accctggatc tggtacgtag ccggatcatc ggacgccagt
      961 acccagcggc cctcgcgggt gataccgatc ggcacgacgt cgtaacgcag cgggtcgagc
     1021 gcggccagca cgctgcccgc ggttatgcag gagatggagt gctcggtgct gcgaccgccg
     1081 aacaggacag ccacccgcag tttgcggtca ccgggctgct gctcttgctg cggttccaca
     1141 gcgccgaccc tactgcgtgt gccgctcggc cttcgcggtc cgacccatca gcctcgcgac
     1201 tccatcgccg ggctgcagtt gctcgtaaag gaccgagacg acgacctcga tgatgggcac
     1261 ctcgatgtcg tgcttgtggg cgagctcaag gatcgacttg gacgacatga ccccttcggc
     1321 cgttgtcgtg gtcgccgcga gcgcggcctc aacactcatg ccacgcccca ggtgttcacc
     1381 gacagtgcga ttgcgcgaca acgggctcga gcacgtggcc accaagtcgc ccaagccggc
     1441 caggccggag aacgtgtgcg ggtcagcccc gagggcgaca ccgaggcgcg ccgtttcagc
     1501 gagtccgcgg gtgatcagcg aggcctgggc gttgtcgccg aagcccatcc cgtcggcgat
     1561 cccgacggcg agcgcgatca cgttcttcac tgcccctgcc agctcgcagc cgacgacgtc
     1621 ggtgttggtg tatggcctga agtaggcggt ctggcaggcg agctgcaagc gctccgcatt
     1681 gtcggcgtcc gtgcaggcaa cgaccgacgc acctggctgt cgctcagcaa tctccctggc
     1741 caggttcgga ccggtgacga tcgcgactcg ctgcgcgggg gcgtcggcaa cctcgcagat
     1801 cacctcgctc atccgcttcg tggtgccgag ctcaacaccc ttaataaggc tgacaaggag
     1861 cgcgtctgct gggaacagtg gcatccagac tgcgaggttc gcccgaagcg tttgggccgg
     1921 gattgcgagc acgacgaggt cggcgctcgc gagtgcctcg gcaggatcga gtgtggcccg
     1981 gagagtatct ggcagctcaa caccttcttg gtaccaagga ttgtgatgct ggctattgat
     2041 cgactcgacg atctccagac ggcgaccgca gagcaccgtt tcggtgccgg cgtcggacaa
     2101 caccatgccg aacgcggtgc cccacgagcc ggtaccgaat accgcggctt tggtcacgcc
     2161 gcgctcaccg gcttggcttc gaagaacgtt gcgggcgggg tttcaccgcg gatgtcggca
     2221 agcagctgcg tcacttcggt catcagcaga tcggtgatct cgcgcaacac gtcgggggta
     2281 ggctcacggc ccttgaatcg ggagagatcg agtggctcgc ccgc
//
LOCUS       DATCBJ010000020         5303 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_255001, whole genome shotgun sequence.
ACCESSION   DATCBJ010000020 DATCBJ010000000
VERSION     DATCBJ010000020.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 5303)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5303)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5303
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_255001"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(48..1079)
                     /locus_tag="VII50_00405"
     CDS             complement(48..1079)
                     /locus_tag="VII50_00405"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HEY5049331.1"
                     /translation="MPCDDRELTELMSEQLRIAVTGSIATDHLMSFPGRFADQLMPDQ
                     LHNVSLSFLVDNLEIRRGGIAANICFGIATFGLRPILVGAVGVDFDDYRSWLERHGVD
                     TVSVHISDLHHTARFVCTTDADQNQIASFYAGAMSDARFIELGPIAARVGGLDLVVVS
                     PNDPLAMLRHTDECRDRGYRFVADPSQQLTRMEGPEIRRLIEGAALLFTNEYEKAMSE
                     AKTGWSDADVLARVGVRVTTLGSRGAIIERVGERPITVAAVPERRLVDPTGVGDGFRA
                     GFFAGLAWGLGLERCAQIGSLLATYVLETVGPQEYEVKPGAFYDRFAETFGDTAAAEI
                     GHLVEAGLN"
     gene            complement(1155..1550)
                     /locus_tag="VII50_00410"
     CDS             complement(1155..1550)
                     /locus_tag="VII50_00410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly accessory protein"
                     /protein_id="HEY5049332.1"
                     /translation="MTAQGDTTTRPDTETGVTDIETGVTGIVLTDHAAAKVKALLEQE
                     GRTDLALRVAVQPGGCSGLRYQLYFDDRSVDGDQVQDFGGVNVVVDKMSQPYLSGASV
                     DFVDTIEKQGFTIDNPNAGGSCACGDSFH"
     gene            complement(1636..2721)
                     /locus_tag="VII50_00415"
     CDS             complement(1636..2721)
                     /locus_tag="VII50_00415"
                     /EC_number="2.7.1.31"
                     /inference="COORDINATES: protein motif:HMM:NF014634.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HEY5049333.1"
                     /translation="MNVWPAARQRVLVAPDSFGGSMSAGEAADAIAAGWREASTTDTV
                     TTVPLSDGGPGFVDVLAAGLQGRLVEVEVGDPLGRSTRGAVLVVDSGGRRTAYVESAQ
                     ACGLHLLRPPERDPSRTTSAGVAALLRAALSAGADRIVVGLGGSGTSDAGSGMIESLA
                     ADAEGMRGVDLVVATDVDNPLLGPLGATAVFAAQKGATPDMMPVLEERLARFADLLGP
                     ELAQVPGAGAAGGLGFALFALGGHRESGADLVLRELDVAGLIAASDLVVTGEGSLDGQ
                     SLRGKLPVRVAAGCLDAAVPCVAIAGRVMLGRREVAATGFAGAYSIEVYAGSLEAAIA
                     LGAPGLRGLAAQVAPLWRLGVPSSGGL"
     gene            complement(2718..3299)
                     /locus_tag="VII50_00420"
     CDS             complement(2718..3299)
                     /locus_tag="VII50_00420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5049334.1"
                     /translation="MGFFDTVLGRTKPVKADLDDLFALPSAALTLQAGLDFAPTGLGS
                     VCFKAAEGAAFARTQTDVQQLLDSSSAGSAPKVELSKDSFGYTWLLVRHDAGDVPGLV
                     TDLHAVNSGLQDAGFGPQLLCSLISFAHADGRRLALVYLYKRGTFYPFAPLGTDQRRD
                     NALEIEVQGTLVNDMKVEPELTRWFPVWGAPGL"
     gene            complement(3302..4228)
                     /gene="htpX"
                     /locus_tag="VII50_00425"
     CDS             complement(3302..4228)
                     /gene="htpX"
                     /locus_tag="VII50_00425"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metalloprotease HtpX"
                     /protein_id="HEY5049335.1"
                     /translation="MARTRFAADRGLSARMVFVMFLLGLLYVVFVGVLIAIHLSPILI
                     VVIAGGFFLFQFFGSDRIALYAMHGHIVTPEEAPELHAIIDRLCALSNTPKPKVAIAD
                     SDVPNAFATGRSQKRAVVCATTGIMRRLDSEELEGVLAHEMSHVAHHDVVVMTIASFL
                     GVLAGLITRFAFYATLFGGGGNQRGRNNNQDSAALVEFVVLIASAIVYAISFFLIRAL
                     SRYRELSADRSGALLTGRPSALASALQKVTGAMGQIPTRDLRSAESFNAFFFTPALAP
                     GFSLSSLFSTHPSLDKRLQQLAEISRELGQGR"
     gene            complement(4243..4521)
                     /locus_tag="VII50_00430"
     CDS             complement(4243..4521)
                     /locus_tag="VII50_00430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5049336.1"
                     /translation="MILRILGEGQFELADHTVEALNGLDNELLAAVESGDDGAFHRTL
                     AELLALVREVGEPVPDDHLGPSELVLPGADSTLEEVRALLGDEGLIPG"
     gene            complement(4518..5294)
                     /locus_tag="VII50_00435"
     CDS             complement(4518..5294)
                     /locus_tag="VII50_00435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspA/IM30 family protein"
                     /protein_id="HEY5049337.1"
                     /translation="MSLIFNAKANKVLDRAEDPRETLDYSYTKQLELLQKVRRGVADV
                     ATSRKRLELQATQLQQQADKYDGQARASLAAGREDLAREALTRKSALTQQISDLQTQH
                     ASLLAEEEKLTLASQRLQAKVDAFRTKKETIKATYTAAEAQTKIGEAFSGISEEMGDV
                     GLAVQRAEDKTQSMQARAGAIDELLASGALIDSTGTSTDDIQLELDRMSSTNDVESQL
                     AAMKGQLASPPATPAIEGAATTATPAAPVAEPATQPEESS"
BASE COUNT          931 a         1801 c         1751 g          820 t
ORIGIN      
        1 ccgcggacag ggcggtcgga aatccggcgc ggcgagagcg ccagcaatca gttcagaccg
       61 gcctcaacga ggtgcccaat ctcagccgcg gcagtgtcgc cgaaggtctc cgcaaagcgg
      121 tcgtaaaacg cgcccggctt cacctcgtac tcctgcggcc ccaccgtctc gagcacgtag
      181 gtcgccagta acgacccgat ctgcgcgcac cgttcaagac cgagacccca cgcgagcccg
      241 gcgaagaaac ccgcccggaa accgtcgccg acgccggtcg gatcgacgag cctgcgctcc
      301 ggcaccgccg ccaccgtgat cggccgctcg cccacccgct cgatgatcgc cccacgcgac
      361 ccgagtgtgg tcacccgcac ccccacccgc gccagtacat cggcgtccga ccagccggtc
      421 ttggcctcgc tcatcgcctt ctcgtactcg ttggtgaaca gcaacgcggc accctcgatc
      481 agccgacgga tctcgggtcc ttccatacgg gtcagctgtt gcgagggatc ggccacgaag
      541 cggtacccgc ggtcgcggca ctcatcggtg tgccgcagca tcgccagcgg atcgttcggg
      601 ctaacgacaa ccaggtcgag gccaccgacc cgggccgcga tggggcccag ctcgatgaac
      661 ctggcgtcgc tcatggcgcc ggcgtaaaac gaggcgatct ggttctggtc ggcgtccgtc
      721 gtgcaaacga accgtgcagt gtggtgcagg tcggagatgt gcaccgacac ggtgtcgacg
      781 ccgtggcgtt cgagccagga tcggtagtcg tcgaagtcga cgccgaccgc tccgacaagg
      841 atcggccgca ggccaaacgt cgcgatgccg aagcagatgt tggccgcgat gccgcctcga
      901 cgaatctcca ggttgtcgac caggaaggac aacgagacgt tgtgcagctg atcgggcatc
      961 agctggtcgg cgaaccgccc aggaaaggac atcaggtgat cggtcgcgat cgacccggtc
     1021 accgcgatcc gcagctgctc tgacatcagt tcggtcaact cccggtcgtc gcagggcacc
     1081 gcatcatcgc aggcacgcca tcatcaggca cgccaaaggc cccgccggac ggcagggcct
     1141 tccgctgacg agaactagtg gaacgaatca ccgcaggcgc acgagccgcc ggcgttcggg
     1201 ttgtcgatcg tgaagccctg cttctcgatg gtgtcgacga agtcgaccga agcaccggag
     1261 aggtagggct ggctcatctt gtcgacgacg acgttgacgc caccaaagtc ttgaacctgg
     1321 tcgccgtcaa ctgagcggtc gtcgaagtag agctggtagc gaaggcctga gcagccaccg
     1381 ggctggaccg ctacgcgcag cgcgaggtcg gtacgaccct cttgctcaag cagagccttc
     1441 acctttgcgg cggcatgatc ggtgagcacg atgccagtga cgccagtctc gatgtcagtg
     1501 acaccggtct cggtgtcagg tcgtgtggtg gtgtcgcctt gagcggtcat cgatgtgctc
     1561 cctggtaaac gcgcgtgtgg tcccatcgtc tcatgaccaa caccagttga ggcgccggtc
     1621 atcccgaatc tggtcctaaa gtccgccact gctcggcacc cccagccgcc acagcggggc
     1681 tacctgcgct gccagcccgc gcagtccagg ggcaccgagc gcgatggccg cctccagcga
     1741 gccggcgtaa acctcgatcg aatacgcgcc ggcgaatccg gtggcggcca cctcacggcg
     1801 tccgagcatg actcgcccgg cgatcgccac gcagggaacc gccgcgtcga ggcagccagc
     1861 agccacccga accgggagct tgccccgcag cgactgaccg tccagcgacc cctcccccgt
     1921 caccaccagg tcggacgccg caatcaggcc ggccacgtcg agctcccgca gcaccaggtc
     1981 ggcgccgctc tcgcgatgac cacccagcgc gaagagcgcg aagccaagcc ctccggcggc
     2041 gccagcgccg ggcacctgcg cgagctccgg ccccaggagg tcggcgaacc tggcgagccg
     2101 ctcctcgagc accggcatca tgtcgggagt cgcacccttc tgcgccgcga agacggccgt
     2161 cgcgccaagc ggcccgagca gcgggttgtc gacatcggtg gcgaccacga ggtcgactcc
     2221 acgcatgccc tcagcgtcgg cggcaagact ttcgatcatg cccgagcccg cgtcgctggt
     2281 gcccgatcca cccaggccga ccacgatgcg gtcggcgccg gcggacagcg ccgcccgcag
     2341 cagcgccgcg acgcccgccg acgtcgtccg gctcggatcg cgttcgggtg ggcgcagcag
     2401 atgcagccca cacgcctgcg ccgactcgac atacgccgta cggcgaccac ctgagtcgac
     2461 gaccaaaacc gcgccgcgag tcgaccggcc gagcggatcg ccgacctcga cctcgacgag
     2521 cctgccctgc agtccggcgg caaggacgtc cacgaagccg gggccgccgt cggacagcgg
     2581 caccgtggtc acggtgtccg tggtcgaggc ctcgcgccag ccggcggcga tcgcgtcggc
     2641 agcctcgccg gctgacatcg atccgccgaa cgagtcgggt gcgacgagca cccgctgccg
     2701 cgcagccggc cacacgttca caagccgggc gcgccccaga ccgggaacca gcgggtcagc
     2761 tccggctcga ccttcatgtc attgacgagc gtgccctgca cctcgatctc cagcgcgttg
     2821 tcgcggcgct ggtcggtgcc aagcggcgcg aacgggtaga acgtgccgcg tttgtagagg
     2881 tagaccagcg ccagccgccg cccgtcggcg tgggcgaagg agatcagcga gcacagcagt
     2941 tgtggcccga acccggcatc ttgcaaacca ctgttgacgg catgcaggtc ggtcacgaga
     3001 ccagggacgt cgcccgcatc gtggcgcacc agcaaccagg tgtagccgaa gctgtcctta
     3061 ctgagctcga ccttcggggc gctccccgcc gacgacgagt cgagcagttg ctggacgtcg
     3121 gtctgcgtgc gggcgaatgc ggcgccctcg gcggccttga agcagaccga cccaaggccc
     3181 gttggcgcga agtcgagccc tgcttgaaga gtcagtgctg cggatggcaa cgcgaacagg
     3241 tcgtcgaggt cggccttcac gggtttcgtc cggccgagga ccgtatcgaa gaaacccatt
     3301 cctacctgcc ctggccgagc tcgcgggaga tctcagcaag ctgctggagc ctcttgtcca
     3361 gcgaggggtg cgtcgagaac agcgacgaga gcgagaagcc tggggcaagt gctggcgtga
     3421 agaagaacgc gttgaagctc tcagccgagc gcaggtcacg ggtcggaatc tgacccattg
     3481 cgccggtcac cttctgcaac gcactcgcga gtgcagacgg acgtcccgtc agcagcgcgc
     3541 ccgaccggtc cgcggacaat tctcgatacc gcgacagcgc cctgatcagg aagaagctga
     3601 tggcatagac gatggccgag gcgatcagca cgacgaattc gacaagtgct gcgctgtctt
     3661 ggttgttgtt gcgcccgcgc tggttcccac ctccgccgaa tagcgtggcg tagaacgcga
     3721 accgggtgat cagaccggcg agcaccccga gaaacgaggc gatcgtcatc acgacgacgt
     3781 cgtggtgcgc gacgtgcgac atctcgtggg ccagcacgcc ctcgagctct tcgctgtcga
     3841 gccgtcgcat gatgccagtg gtcgcgcaga ccaccgcccg cttctggctg cggccggtgg
     3901 caaacgcatt gggaacgtcg ctgtcggcta tcgcgacctt cggcttcggg gtgttcgaga
     3961 gcgcgcagag ccggtcgatg atggcatgga gttcgggtgc ctcttcaggc gtcacgatat
     4021 ggccgtgcat cgcgtataac gcgatccggt cggacccgaa gaactggaac aggaagaagc
     4081 cgcccgcgat caccacgatg agaatcggcg acaggtggat cgcgatgagc acgccgacga
     4141 acacgacgta cagcagcccg agaaggaaca tgacaaagac catgcgagcc gacagaccgc
     4201 ggtctgcggc aaaacgggtt cgggccataa gagggacgcc gatcagccag ggatgagtcc
     4261 ctcgtcgccg agcagcgcac gcacctcctc caaggttgaa tcagcgccgg gcaggaccaa
     4321 ctcggatggg ccgaggtggt cgtcgggcac cggttccccg acctcacgga caagggcaag
     4381 cagctcggca agggtgcggt gaaacgcgcc gtcatcgcca ctctcgactg cggcgagcag
     4441 ttcgttgtcg aggccgttca acgcttcgac cgtgtgatca gcgagctcga actggccttc
     4501 accgagaatg cgcaggatca tgacgactcc tccggctgcg tggcgggctc ggcgaccggc
     4561 gctgctgggg tcgcggtggt ggccgcgccc tcgatggccg gggtcgccgg cgggctggcg
     4621 agctggccct tcatagcggc cagctgcgac tcgacatcgt tggtgctcga catgcggtcg
     4681 agttcgagct ggatgtcgtc ggtgctcgtg ccggtcgagt cgatcaaggc acccgaggca
     4741 aggagctcgt cgatcgcacc ggccctggcc tgcatgctct gcgtcttgtc ttcggcccgc
     4801 tgcaccgcca ggccgacatc gcccatctcc tcggagatgc cggagaaggc ttcgccgatc
     4861 ttcgtctggg cctcggccgc cgtgtaagtg gccttgatcg tctctttctt ggtacggaaa
     4921 gcgtcgacct tggcctgcag acgttgcgag gcgagcgtca gcttctcctc ctccgccagc
     4981 agcgaggcgt gctgagtctg gaggtcgctg atctgctggg tcagcgccga cttgcgggtg
     5041 agcgcttcgc gggcgaggtc ttcgcgaccc gccgccaacg acgcgcgcgc ctggccgtcg
     5101 tatttgtcgg cctgctgctg cagctgggtg gcctgcagtt cgagtcgctt gcgcgacgtg
     5161 gccacgtcgg caacgccacg ccgcaccttc tgcagcagct cgagctgttt ggtgtagctg
     5221 tagtccagcg tttcgcgcgg gtcttcagcg cggtcaagga ccttgttggc cttggcattg
     5281 aagatgagcg acatgcgctt cat
//
LOCUS       DATCBJ010000021         3336 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_548874, whole genome shotgun sequence.
ACCESSION   DATCBJ010000021 DATCBJ010000000
VERSION     DATCBJ010000021.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 3336)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3336)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3336
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_548874"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            198..464
                     /locus_tag="VII50_00440"
     CDS             198..464
                     /locus_tag="VII50_00440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5049338.1"
                     /translation="MERQVECFATVVKVWKRGSMETRGFRVEIIARRTSTTMYRSAVK
                     STEAEALTLACKFLKRNPSLVLAPQLGGGSTPPATAILAAMVAS"
     gene            461..970
                     /locus_tag="VII50_00445"
     CDS             461..970
                     /locus_tag="VII50_00445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5049339.1"
                     /translation="MTHSINTVRAFLQLQRESDEPDEILAILKTQDGKQLTKRILAKL
                     PGGEARWRLSQFATMTHLETRDYGRTGGNAGIHLLIAYQTTNVTIDAAQIEEKNPGYF
                     AGRRARNASRDALADDTGRLNNMANTLNVYEHAKAQLDEAKDNLDALIQHGAPFAAEC
                     NAHRRKGAA"
     gene            970..1422
                     /locus_tag="VII50_00450"
     CDS             970..1422
                     /locus_tag="VII50_00450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5049340.1"
                     /translation="MDTRHEVTEENAPKFLEWIAKRGGVAIWPSVDLSDPSASMSSPA
                     LTTEGEPTPKPHWKLALEPSRVITDASLIDVMTWKEVKRFHVGVRRGDGLSFTLTDGA
                     TRKLRAAVAKAGENATYRFDYETQEAVIVVPDKVVPLDKWVDEAGVPR"
     gene            1419..1745
                     /locus_tag="VII50_00455"
     CDS             1419..1745
                     /locus_tag="VII50_00455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5049341.1"
                     /translation="MSTVAGLKFACGCCSDGCICVTHCTTTPVVLNGSLVRKLVVCRK
                     HATTKSKRGVTIEMHVTRDQRRAINHQVGKRGEATDAEVAAWIKKAAGADLQVLEVEY
                     DNAEEK"
     gene            1811..2038
                     /locus_tag="VII50_00460"
     CDS             1811..2038
                     /locus_tag="VII50_00460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5049342.1"
                     /translation="MFEVECFVAPDGKVVPVPSGEYGYTGYAHVVGHDVYETRSDAET
                     EAFKRAEKDVKDKTKWLREAESYLARLRASV"
     gene            2084..2272
                     /locus_tag="VII50_00465"
     CDS             2084..2272
                     /locus_tag="VII50_00465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5049343.1"
                     /translation="MVDKQGHEHQVQTAIRIPESWVDRLDKLAKIMSAPGVPATRAGA
                     LRSALYRGIVEMEKEKKR"
     gene            2269..2922
                     /locus_tag="VII50_00470"
     CDS             2269..2922
                     /locus_tag="VII50_00470"
                     /inference="COORDINATES: protein motif:HMM:NF021759.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT-like domain-containing protein"
                     /protein_id="HEY5049344.1"
                     /translation="MSNVRWACPTSDSGHPAVIAPSRPRKDDVRRFCLACSGASGRLV
                     ARVPPVLERKREARRVHEVERLVKVAVREREREASYYTVAGVNLMDEMCRMIQAKAFI
                     GTPLRHRGRRPTLIVRRRSSKPRRFGFAQGGRWRIIISAYPDITAHDVRETLCHELAH
                     LATTGTVHGIAWKTMFRNASEELFGVRPRVELRYHGEVTGLLKARDEREAAESAGPT"
     gene            2928..>3336
                     /locus_tag="VII50_00475"
     CDS             2928..>3336
                     /locus_tag="VII50_00475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5049345.1"
                     /translation="MMLLVVEVPTLDHAGYIQKEAERLVGLAVRKALSELPRELMDKA
                     YVSSVTATPFGEGSTCALLADEVQRLHAVIDVIKKPHEFSTPPAVGGGRGIEIKVVDD
                     KCELRVVRSKTKEVVHRVDVSGRSDGMIEKVMSG"
BASE COUNT          666 a         1025 c         1138 g          507 t
ORIGIN      
        1 ggccgtcatc cgcaagcacg gcgtcgcgcc acacccggcc tacgtgctcg gctggggtcg
       61 cgtctcctgc gccgcctgca tcttcggctc gcccagtggg tgatgccccg tggggcgttc
      121 ggcgagagct gcgggccgac ctgatagtgg ttgacaacgt ataccaagtg attacgcttg
      181 gtgttggagg taggaaaatg gaacgccagg tagagtgctt cgcaactgtc gtcaaagtct
      241 ggaagcgcgg atcgatggag acgcgcgggt ttcgggtcga gatcattgcc cgccgcacgt
      301 cgaccacgat gtaccggtcg gcggtaaagt cgaccgaggc cgaagcactg acgctggcgt
      361 gcaaattcct gaagcgcaac ccgagcctgg tgctcgcgcc acagctcggc ggcggtagta
      421 ctcctccggc gacggcgatt ctggccgcga tggtggcgtc atgacccact cgatcaacac
      481 cgtccgcgcc ttcctccagc tccagcgcga gtccgacgag cccgacgaga tcctcgccat
      541 cctgaagacc caggacggca agcagctgac caagcgcatc ctcgccaagc tccccggcgg
      601 cgaggcgcgg tggaggctca gccagttcgc cacgatgacg cacctggaga cgcgcgacta
      661 cggccgcacc ggcggcaacg ccggcatcca cctgctcatc gcctaccaga ctacgaacgt
      721 caccatcgac gccgctcaga tcgaggaaaa gaacccgggt tacttcgccg gccggcgagc
      781 gcgcaacgct tcgcgtgatg cgctagcaga tgacaccggc agactgaaca acatggccaa
      841 cacgctcaac gtgtacgaac acgcgaaggc gcagctcgac gaagcgaaag ataacctcga
      901 cgcactcatc caacacggcg cgccgttcgc tgccgaatgc aacgcgcaca ggcgcaaagg
      961 agcagcgtga tggatacgag acacgaggtg acggaagaga acgccccgaa gtttctggag
     1021 tggatcgcca agcgcggcgg cgtggccatc tggccgagcg tcgacctgtc cgatccgagc
     1081 gcgtcgatgt cgtcgccggc tctgacgacc gaaggcgagc cgacgcccaa gccgcactgg
     1141 aagctggcgt tggaaccgtc gcgggtaatc acggacgcca gcctgatcga cgtgatgacc
     1201 tggaaagagg tcaagcggtt ccacgttggc gtgcggcggg gcgacgggct gtcgttcacg
     1261 ctgacggacg gcgccacccg caagctgcgc gcagcggtcg ccaaggccgg cgagaatgcg
     1321 acgtatcggt tcgactacga gacgcaagag gcagtcatcg tggtgcccga caaggttgtg
     1381 ccgctcgaca agtgggtcga tgaggctggg gtgccgcgat gagtaccgtc gctggtctca
     1441 aattcgcgtg cgggtgctgc tcggacgggt gcatctgtgt cacgcactgc accaccactc
     1501 ctgtcgttct caacggatcg cttgttcgaa agttggtcgt gtgccgcaag cacgcgacga
     1561 cgaagagtaa gcgtggtgtc acgatcgaga tgcacgtcac gcgcgaccag cgccgagcga
     1621 tcaaccacca ggtcggtaag cgcggcgaag cgacagacgc ggaggtggcg gcgtggatca
     1681 agaaggcggc tggagctgac ctgcaagtgc tggaagtgga atacgacaac gcggaggaaa
     1741 aatgaccacg gtggcatcca caacgacaat cttctgggcc attcggaacg cctggcgcga
     1801 aggcgccccc gtgttcgagg tcgagtgctt cgtcgcgccg gacggtaagg tcgtcccggt
     1861 cccgagcggc gagtacggct ataccggcta cgcccacgtt gtcgggcacg acgtctacga
     1921 gaccaggtcc gacgccgaga ccgaagcgtt caagcgtgct gagaaggacg tgaaggataa
     1981 gaccaagtgg ctgagggagg cggaatccta cctcgcgcgg cttcgtgcgt cggtttgaca
     2041 gaagcggttg acggtggtat acaattaggt acggagacac tcaatggtag acaagcaggg
     2101 tcacgagcat caggttcaaa ccgccatccg catcccggag tcgtgggtcg accgtctcga
     2161 caagctcgcc aagatcatgt cggcgccagg tgtaccggcc acacgagctg gcgcgctgcg
     2221 gtcggcgctg taccggggga tcgtcgagat ggagaaggag aagaagcgat gagcaacgtc
     2281 cgttgggcct gccccacctc cgactccggc cacccggccg tcatcgcgcc gtcgcggccg
     2341 cgcaaggacg acgtgcggcg cttctgcctg gcgtgcagcg gcgcgtcagg gcggctcgtt
     2401 gctcgcgtcc cgcctgtgct ggagcgcaag cgcgaagcgc ggcgagtcca cgaggtggag
     2461 cgcctggtga aggtcgccgt cagggagcga gagcgcgagg cctcctacta caccgtcgct
     2521 ggggtgaatc tcatggacga gatgtgccgg atgattcagg cgaaggcgtt catcgggacg
     2581 cccctgcgtc atcgaggaag acgcccgacg ctcatcgtcc gcaggcgcag ctccaagccg
     2641 aggcggttcg gcttcgctca aggcggccgg tggcgcatca tcatctcggc ctacccggac
     2701 atcacagcac acgacgtccg cgagacgctc tgccacgagc tggcgcacct ggcgacgacc
     2761 ggtaccgtgc acggcatcgc gtggaagacc atgttccgca acgcgtcgga ggagctattc
     2821 ggcgttcggc cgcgcgtcga gcttcgctac cacggcgagg tcacggggtt gctgaaagcg
     2881 cgagacgagc gggaagccgc agagagtgcg gggccaacgt gaaggcgatg atgctcctcg
     2941 tcgtcgaggt cccgacgctt gaccacgccg gctacatcca gaaggaagca gagcgcctcg
     3001 tcgggctcgc ggtacgcaag gcgctgagcg agctaccgcg cgagttgatg gacaaggcgt
     3061 acgtctccag cgtcactgcc acaccgttcg gtgaaggctc gacgtgcgcg ctactggcgg
     3121 acgaagtaca gcggctgcac gccgtcatcg acgtgatcaa gaagccgcac gagttcagca
     3181 cgccgccagc ggtcggcggt ggccggggga tcgagatcaa ggtggtcgac gacaaatgcg
     3241 agctacgggt ggtccgcagc aagaccaagg aggtcgtaca tcgtgtcgat gtctccggca
     3301 ggtccgacgg catgatcgag aaggtgatga gcggca
//
LOCUS       DATCBJ010000022         2255 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_181590, whole genome shotgun sequence.
ACCESSION   DATCBJ010000022 DATCBJ010000000
VERSION     DATCBJ010000022.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2255)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2255)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2255
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_181590"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1122
                     /locus_tag="VII50_00480"
     CDS             <1..1122
                     /locus_tag="VII50_00480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HEY5049346.1"
                     /translation="LADAVRVGASDIHVEQQADGIWIRHRIDGVLRDVTRMPKSSASA
                     LVSRLKIVAGMDIAQRRLPQDGRMKITANGIAVDARVSTLPSVNGEKVVIRLLPDADR
                     ITPLDRLGMEPAQLQVLVSAATASQGLVLITGPTGSGKTNTLYSLLAEVATREKNVIT
                     LEDPVEIQLPGITQVQINERAGMTFARGLRSMLRQDPDVILVGEVRDSETALLALEAS
                     LTGHLVLSTLHTNNACAAVTRLVEMGVEPYLVASSLALVVGQRLVRRPCAACAEPYKP
                     SAELLSRLRLTAADLRGALPMRGTGCLECNDSGYRGRVGIFEVLPVNADLREVLLTTP
                     TEGEVTKVAVAMAMVTMRAAGISKAKRGETTYEEVLRVT"
     gene            1140..1310
                     /locus_tag="VII50_00485"
     CDS             1140..1310
                     /locus_tag="VII50_00485"
                     /inference="COORDINATES: protein motif:HMM:NF037605.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5522 domain-containing protein"
                     /protein_id="HEY5049347.1"
                     /translation="MVSDSDSARIAAVHAKAVADGEDGYLDPSTGLFVLTEDYLTRNG
                     SCCGNGCRHCPY"
     gene            1455..>2255
                     /locus_tag="VII50_00490"
     CDS             1455..>2255
                     /locus_tag="VII50_00490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEY5049348.1"
                     /translation="MSKRGWLLFTAMAVIWGIPYLLIKVSLRDLTPGTLVFARTGIAA
                     ILLVPLAAKRGMLRPLLSKWRVLLLYTVIEIAGPWFLLSSAEQRLSSSLAGLLVASVP
                     LIGAIAAFIVGGVGGGHDDRPDARRVLGLLVGLGGVAVLVGVDVSGSDVWAVVAIAIT
                     AIGYATGPMLISRKLADLPTLGVVAASLGLTAIGYAPVAATQWPTHMDAKVLGSVLGL
                     ALICTALAFMVFFALIAEVGPARATVITYVNPAIALLLGVLLLNEPFTV"
BASE COUNT          359 a          753 c          721 g          422 t
ORIGIN      
        1 cttgcggatg ccgtgcgcgt tggcgcgagc gacatccacg tggagcagca agccgacggg
       61 atctggatcc ggcaccgcat cgacggcgtg ctgcgtgacg tcacccggat gcccaagagt
      121 tcggcgtcgg cccttgtcag ccgactgaaa atcgttgcag ggatggacat cgcacaacgc
      181 cgcctgcccc aggacggccg gatgaagatc accgcgaacg gcatcgccgt cgacgcacgc
      241 gtgagcacgt tgccctcggt caacggagaa aaggttgtca tccggctgct gcccgacgcg
      301 gaccgcatca cacctctcga caggctcggc atggagcccg cccagcttca agtgctcgtg
      361 tcggcagcga cagcgtcgca gggtctggtg ctgatcaccg gtccgaccgg atccggcaag
      421 accaacacgc tgtactcgtt gcttgccgag gtagcgaccc gcgagaagaa cgtcatcacg
      481 ctcgaggatc cagtagagat ccaattgccg ggcatcactc aggtgcagat caacgaacga
      541 gccggcatga ccttcgctcg cggcctgcgc tcgatgttgc gtcaggaccc cgacgtcatc
      601 ttggtcggag aggttcgaga cagcgaaacc gcgctccttg cactcgaggc ctcactgacc
      661 gggcacctcg tgctgtcgac gctgcacacc aacaacgcct gcgccgcggt gacccgcctc
      721 gtcgagatgg gcgttgagcc ttacctcgtc gcgtcgtcgc tcgccctcgt cgtcgggcaa
      781 cgattggtgc gccgcccctg cgccgcatgt gccgagccct acaagccctc ggctgagttg
      841 ctcagccgct tgcggctcac cgcggccgat ctgcgaggcg ccctacccat gcgcggtaca
      901 ggttgcctgg agtgcaatga ctccggttac cgcgggcggg tgggcatctt cgaggtgctg
      961 ccggtcaacg ctgacttgcg cgaggtgctc ctgactaccc cgaccgaggg tgaagtcacc
     1021 aaggtcgccg tcgcgatggc aatggtgacg atgcgcgcgg ccggcatttc aaaggccaag
     1081 cgcggcgaga ccacgtacga agaggtcttg cgcgtcacgt aagcgcctag catcgacgtg
     1141 tggtttccga ctccgatagc gcgcgcatcg ccgccgtcca tgccaaagct gtggcggacg
     1201 gcgaggacgg ctatctcgac cccagcaccg gtcttttcgt tctgaccgag gactacctga
     1261 cccgcaacgg ctcctgctgc ggcaacggct gccggcactg cccttactga ccggtcacgc
     1321 gctgcgattg ctgaacggtc gcgttcagcg acaccttcac aaacgcggat tctccttacg
     1381 ctgcagccga aatccgcgag aattgtgtca catatacgtg agagggttga gctctgcagc
     1441 gatgagggag cggcatgtcg aagcgtgggt ggctgctatt cactgccatg gcggtgatct
     1501 ggggcatccc ctacttgctc atcaaggtct ctctgcgcga cctcacgccg ggcaccctcg
     1561 tcttcgcccg taccggcatc gcggcgatct tgctggtccc actcgccgcc aagcgcggca
     1621 tgctgcgacc gctgctgtcg aaatggcggg tcctgctgct gtacacggtg atcgagatcg
     1681 ccgggccctg gttcctgttg tcgagcgcgg agcaacgcct ctctagttcg ctggctggct
     1741 tgctcgtcgc gtccgtaccg ttgatcggag cgatagccgc attcatcgtg ggcggcgtcg
     1801 gcggcggcca cgatgatcgg ccggatgccc gccgcgtgct tggcctgctg gtcggtctcg
     1861 gcggggtggc tgtgctcgtc ggcgtcgacg tcagcggcag cgatgtatgg gcggtcgtgg
     1921 cgatcgcgat taccgccatc ggctacgcaa cgggcccgat gctgatctct cgcaagctgg
     1981 ccgacctgcc gacgcttggt gtcgtcgctg cctccctcgg cctgaccgcg atcgggtacg
     2041 cgccggttgc tgccacccag tggccgaccc acatggacgc gaaggtgctc ggctcggtcc
     2101 tcggtctcgc actcatctgc acggcgctcg cgttcatggt cttcttcgcg ctcatcgccg
     2161 aagttggccc ggccagagcg acggtgatca cttacgtgaa cccggccatc gcgctgctac
     2221 tgggcgttct gctgctgaac gagcccttca cggtc
//
LOCUS       DATCBJ010000023         2155 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_512623, whole genome shotgun sequence.
ACCESSION   DATCBJ010000023 DATCBJ010000000
VERSION     DATCBJ010000023.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2155)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2155)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2155
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_512623"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..254)
                     /locus_tag="VII50_00495"
     CDS             complement(<1..254)
                     /locus_tag="VII50_00495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5049349.1"
                     /translation="MSGDGRGALVTAAAAVSLVVVGVVAGFIGIIEATALVRLGPVHI
                     SVGAVISGVANCALGLLAVWGLGSREAAALPALGWFVMVAV"
     gene            complement(264..>2155)
                     /locus_tag="VII50_00500"
     CDS             complement(264..>2155)
                     /locus_tag="VII50_00500"
                     /inference="COORDINATES: protein motif:HMM:NF013593.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEY5049350.1"
                     /translation="LSLFVGLPTLFLWYRIIEHRPFNVFALPHLNLTIVALFFVPALL
                     LVGMVVMYASYGRSPHFVVRPEQIDVRLSDVVGIDLVKAEVVRSLNLFLAHKAFSSEM
                     GGRPRRGLLFDGPPGTGKTYTAKALAAEANVPFLFATATSFQSSMQGATQRKVRSYFK
                     ALRALARKEGGAIGFIDEFDALGGSRSGVAASTPSGALDGMPMSCGGLEGLANVVSPA
                     TQQTQFVGSGDLGMAVQELLVQLQSFDQPTAGERLVGKITDAANLLLPATHQLKKPAV
                     KSSNIMLIASTNRPDSLDPALMRPGRFDQRLTFDRPDKVGRRELIAHFLARKSHAAEL
                     DDPQRREALAAVTQGYSPAMLEGMFDEALINAVQEDRRAMTWLDVEHARLDSEIGLGQ
                     PVLYTDHEAKLIATHESGHATIAWLVAPGRRLEVLSIIKRRSALGLLAHGDREEVYLQ
                     SRSELLGLIAISMGGQSAEELFFGDVSTGPSGDLLAATRVAASMVGSCGMEGSLVSFG
                     AVQNSGFSDTNIVGRVLGDKDGRAAVEEVLQQQKLRAKALLEDNRHLVMALRDALLER
                     HELIGTEITDVLEQAQHEHAAGHTRSFGSGTTPTALAAVIDLRETALPTQATGPRDST
                     IEPAG"
BASE COUNT          389 a          725 c          708 g          333 t
ORIGIN      
        1 accgccacca tgacgaacca gccgagagcc ggcagcgcgg cggcctcgcg gctgccaaga
       61 ccccacacgg cgagcagccc aagcgcgcag ttcgcgacgc ccgagatgac tgcaccgacg
      121 gagatgtgca ccgggccgag ccggaccagc gcggtggcct cgatgatgcc gatgaacccc
      181 gccacgacgc cgacaacaac gagtgacacg gccgcggccg ccgtcaccag ggcgccgcgc
      241 ccgtcaccgc tcaccgcggc aggctatccg gcaggttcga tcgtggagtc tcgcggaccg
      301 gttgcctgcg tcggcagcgc tgtctcacgc aggtcgatga ctgcggccaa ggcggtaggc
      361 gtcgtgcccg agccgaagct gcgggtgtgg ccagccgcgt gctcgtgttg cgcctgctca
      421 agcacatcgg tgatctcggt gccaatcagc tcgtgccgct caagcagcgc gtcacgaagg
      481 gccatgacca ggtggcggtt gtcctcgagc aaagccttgg cccgcagctt ttgttgctgg
      541 agcacttctt cgaccgccgc gcggccatcc ttgtcgccga gcacgcggcc gacgatgttg
      601 gtgtcggaga atccgctgtt ctggacggcg ccaaaggaaa ctaacgagcc ttccatgccg
      661 caggaaccga ccatgctcgc tgctacccgg gttgccgcga gcaggtcacc gctcggcccg
      721 gtcgacacgt ctccgaagaa gagctcctca gcggactgcc cacccatcga gatcgcaatg
      781 aggccaagca gctcgctgcg cgactggagg tagacctctt cacggtcacc gtgcgcgagt
      841 aaaccaagtg cgctgcgacg cttgatgatg ctcagcacct cgagcctgcg gcccggagca
      901 accagccacg cgatcgtggc gtggccggac tcgtgagtcg cgatcaactt cgcctcgtgg
      961 tcggtgtaca gaaccggctg gccgagcccg atctcgctgt caagccgcgc gtgctcgacg
     1021 tcgagccacg tcattgccct gcgatcctcc tgcacggcat tgatcagcgc ctcgtcgaac
     1081 atcccctcga gcatcgccgg gctgtacccc tgggtgaccg cggcgagcgc ctcgcgccgc
     1141 tgcgggtcgt cgagttcggc cgcatgggac ttgcgagcaa ggaagtgcgc gatcagttca
     1201 cggcggccga ccttgtcagg cctgtcgaac gtgagtcgct ggtcgaaccg gccgggccgc
     1261 atcaacgcgg ggtcgagcga gtcgggccgg ttcgtcgagg cgatcaacat gatgttcgac
     1321 gacttgacgg ccggcttctt cagctggtga gtcgccggga gcaacaggtt tgccgcgtcg
     1381 gtgatcttgc cgacgagccg ctcgccagct gtgggctgat cgaaggactg cagctgcaca
     1441 agaagttctt gcaccgccat gcccaggtcg ccgctgccga cgaactgcgt ctgctgggtc
     1501 gcaggcgaca cgacgttggc taggccctcg agaccgccac acgacatcgg cattccgtcg
     1561 agggcgcccg agggcgtcga cgccgcgact ccggagcgcg atccaccaag tgcgtcgaac
     1621 tcgtcgatga acccgatcgc gccgccctcc ttgcgagcca gcgcgcgcag cgccttgaag
     1681 tacgagcgca ccttccgttg cgtcgcgccc tgcatgctcg actggaacga ggtcgcggtc
     1741 gcgaacagga acggcacgtt ggcttcggcc gcgagggcct tcgccgtgta ggtcttaccg
     1801 gtgccgggcg ggccgtcaaa gagcaagccg cgccgtggcc ggccgcccat ctcactcgaa
     1861 aatgccttgt gtgccaagaa aaggttgagc gaccgcacga cttcggcctt caccaggtca
     1921 atgccgacaa catcagaaag gcggacgtcg atctgctcag gccgcacgac aaagtgcggc
     1981 gagcgcccgt agctcgcgta catgacgacc atgccgacta gcagcagcgc gggcacgaag
     2041 aacagcgcga cgatcgtcag gttgaggtgt gggagcgcga acacgttgaa cggccggtgc
     2101 tcgataatgc ggtaccaaag gaacagcgtc gggagtccga cgaacaacga cagcc
//
LOCUS       DATCBJ010000024         2121 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_512565, whole genome shotgun sequence.
ACCESSION   DATCBJ010000024 DATCBJ010000000
VERSION     DATCBJ010000024.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2121)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2121)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2121
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_512565"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            136..687
                     /locus_tag="VII50_00505"
     CDS             136..687
                     /locus_tag="VII50_00505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5049351.1"
                     /translation="MLKNTSICGSTNDLGVRVYVGSVVVALALAILLPRQAIEIVPPG
                     TVSMLFPVDGLAVGETVEAVCEADGDGDGDGDGDTETDGEGDADVDGVPVVVGVGVGV
                     GVTVGVGDTDGATGPPTAMPLMQVKTYSIPGGLGRTMDGDGDFGGGLVEGVAGGEVDG
                     VTLTGVVFEVVGVGGVGGGVGAT"
     gene            1099..1809
                     /locus_tag="VII50_00510"
     CDS             1099..1809
                     /locus_tag="VII50_00510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEY5049352.1"
                     /translation="MSTVSHVPRLLLIEDDAAIRTSLTRSLSERGHAVASAPTAMAGL
                     QAVVDTAPDLVVLDLGLPDLDGGKLIQMVRAVSKVPIIVATARDDQAEMVRILDWGAD
                     DYVVKPFASDHLEARIRAVLRRMASGDAAQAVVVGGLRIDQRTREASLDGDKLELTRK
                     EFDLLLYLAEHEGEVVSKRVLLAEVWQQAYGGADKTVDVHLSWLRRKLGESAAEPRYL
                     LSVRGVGVKLVGPTEPDS"
     gene            1828..>2121
                     /locus_tag="VII50_00515"
     CDS             1828..>2121
                     /locus_tag="VII50_00515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5049353.1"
                     /translation="MRNRLLILAAATTSLVIVAFLVPLSVLVRNLAHQRALNDGLKES
                     QQVVTAVQTGERTKAAVAQAVSGSPAGIVVSVRLGDGQWIGLVPPDPDSPALRL"
BASE COUNT          311 a          649 c          795 g          366 t
ORIGIN      
        1 accgccaccc acgacagcaa gtttcatccg cgcaactcct cggcaggtga ttgtaaagtt
       61 ccctgcctac cacgctagtg cggtggcttc gacggtgtca atgctgatgg cgctcggctc
      121 gggtgccttt agttcttgct gaagaacacg tcgatttgtg ggtcgaccaa cgacctcggg
      181 gtcagggtgt atgtgggatc ggtggtcgtc gcgctggcgt tggcgatctt gttgccgagg
      241 caggcgatcg agatcgtgcc gccgggtacg gtctcgatgc tcttcccggt cgatgggctg
      301 gcggtgggcg agactgtcga ggccgtgtgc gaggccgacg gcgacggcga cggcgacggt
      361 gatggcgaca cggaaaccga cggcgagggt gatgcggacg tcgacggcgt gccggtcgtc
      421 gtcggagtgg gcgtcggcgt cggcgtcacc gtcggagttg gtgatacgga cggcgccacc
      481 ggcccaccca cggcgatgcc gttgatgcag gtgaagacgt actcgatacc gggcggcctc
      541 ggaagaacga tggacggcga cggcgacttc ggcggcgggc tggtggaggg cgttgcgggc
      601 ggcgaggtcg acggcgtcac cttgacgggt gtggtcttcg aggtggtcgg tgtcggcggt
      661 gtcggcggcg gtgtgggtgc gacgtaaggc ggcgtcggta ccgaggtggg cgtccgggcc
      721 gcggaacttg ccgaggcggc ggaggctgat tgcgccgcca cctgtctgtt gacgacgtcc
      781 tgcgggacga tcagaccctg gctgccactg acttcgcgcc cgacccgttc gactgccgtc
      841 aatgtcacgg ccgtgatcag aacgcaggag agcagccagc ccagcgccag ggtgagcggc
      901 cgacccggcc gtcgccgacc gccatcggcg ggcgcaccag cgacggcggc tgcgcctcgg
      961 cgccaccacg ggcgctgatc aggggtctgc tgctcgggca catgtccatt ctcggccgat
     1021 ctgcgtcggt ccggagttaa cgcgctctca aggtgccggc caaatcttgg cccggccggt
     1081 gcggcatcgg cgcctgcatt gagtaccgtc agtcacgtgc cccgcctgct gctgattgag
     1141 gatgacgccg cgatccgtac gtcgctcacg cgctcgctct ctgagcgcgg ccacgcggtc
     1201 gcttccgcgc cgacggcgat ggccggcctc caagcggtcg tcgacacggc tcccgacctg
     1261 gtcgtcctgg atctggggct gccggatctc gacggcggca agctcatcca gatggtgcgg
     1321 gcggtcagca aggtgccgat catcgtggcc accgcccgcg acgatcaggc cgagatggtc
     1381 cggattctcg actggggcgc cgacgactac gtcgtcaagc cgttcgcatc tgaccacctc
     1441 gaggcgcgca tcagggcagt cctgcggcgg atggccagcg gggacgccgc ccaagccgtg
     1501 gtggtcgggg gtctgcggat cgaccagcgg accagggagg cgagtctcga cggcgacaag
     1561 ctcgagctga ctcgcaagga gttcgacctg ttgttgtacc tcgccgagca tgaaggcgag
     1621 gtcgtgagca agcgggtgct gctggcggag gtgtggcagc aggcatatgg aggcgccgac
     1681 aagactgtcg acgtccactt gtcgtggttg cgccgcaagc tcggcgagtc cgcggccgag
     1741 ccgcgatacc tgctgagtgt gcgcggagtc ggcgtcaaac tggtcggccc gaccgagccg
     1801 gacagctagg ccgacggggc cggttaggtg cgcaacaggc tcttaatcct tgcggcggcg
     1861 acgacatcgt tggtgatcgt cgccttcctc gttccgctgt cggtccttgt gcgcaacctc
     1921 gcgcaccagc gggctctgaa cgacggactg aaggagtccc agcaggtcgt taccgcggtc
     1981 cagacgggcg agcggacgaa ggcggcggtc gcacaggcgg tgtcgggttc gccggcgggg
     2041 atcgtggtgt cggtacgact tggcgacggt caatggatcg gactggtgcc gcccgatccc
     2101 gactctccgg cgttgcggtt g
//
LOCUS       DATCBJ010000025         4158 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_181109, whole genome shotgun sequence.
ACCESSION   DATCBJ010000025 DATCBJ010000000
VERSION     DATCBJ010000025.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 4158)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4158)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4158
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_181109"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(124..1434)
                     /locus_tag="VII50_00520"
     CDS             complement(124..1434)
                     /locus_tag="VII50_00520"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014167165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HEY5049354.1"
                     /translation="MSVFDDLVGQERVVALLRSVAISAAEVAWASDLGGDLFGALSDA
                     SVVLPEAVAAAAPGAAMTHAWLFTGPPGSGRSVAARSFAAALQCTAAMNGGGDIEPGC
                     GVCLGCHTVLAGTHADVEIVAPQGLSLGVREARDLVSRAALAPSGGHWQVIIIEDADR
                     LTEGAANVLLKAIEEPSPRTVWLLCVPSAEDLVVTIRSRCRVVALRTPPTDAVASVLV
                     LRDGVSPSVAQFAARAAQGHVGRARRLATDEAARSRRHDVLQLPSALADVGGCLAAAG
                     ALVKAVGAESAEVTSALDEAETASYKEAFGEGGTATGLGRGAAGGGGGAARGALRSAA
                     AALKDLEGRQKSRATRTQRDALDRALTDLAGFYRDVLVVQLGAGVDIANTDETAAISA
                     LAASSSAESTVRRLSAVLACRTSLDANANPLLTVEALTLALRAS"
     gene            complement(1431..3554)
                     /gene="tmk"
                     /locus_tag="VII50_00525"
     CDS             complement(1431..3554)
                     /gene="tmk"
                     /locus_tag="VII50_00525"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HEY5049355.1"
                     /translation="MPPPAPLVEAARAAATEVGGFRGVLAIKAFRKLWIALSLSSFGD
                     WLGFLAQTALAAELAAGHTYLVQNSSVGLVVFVRLLPAVLLAPLAGAFADRLDRRLTM
                     VIADIGRFSLYVSIPIVHTLWWLFIATFLIETLSLFWIPAKEATVPNLVPREQLEQAN
                     QLSLLTTYGSAPVAALVFALLSLFTGVLGHALPFFTTNRIDLALYFDASTFLVSAGTI
                     FTLREVSHQGGRRRRAELEDGAAGDVVPISQPSVLRAIAEGWQFIGQTKVVRGLVVGM
                     LGAFAAAGVVVGLAKRYTLDLGGGDPAYGAMFGSVFVGLAVGMFLGPKFFRRLSRRRL
                     FGVAIAASGLTLALTAVVHNLVLVVLGALVMGAFSGVAWVTGYTLLGLEVEDEKRGRT
                     WATLQSLMQVDLLLMVVAGPFISGAIGVHTLHIKRVVLRQDGAACTLLGAGLIAFVVG
                     VTAYRQMDDRDVPLWRDLIGALRGWRPLEGRGVTTGLFVAFEGGEGAGKSTQCKLLAS
                     WLTERGYEVVTTREPGATPIGRQLRHLLLNPDNGEIAPRAEALLYAADRAQHVATVIR
                     PALARGAVVVSDRYVDSSLAYQGVGRSLSTGEIERLSRFATSGLRPDLTVLLDVPVTD
                     GLARTSVRDELPDRLEAESAAFHERVRAAFRELAEASPDRYLVIDATLQPATIHQLIV
                     RRIADFVPELSPDLQPTVQLKVGRR"
     gene            complement(3627..>4158)
                     /locus_tag="VII50_00530"
     CDS             complement(3627..>4158)
                     /locus_tag="VII50_00530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019044598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="topoisomerase C-terminal repeat-containing
                     protein"
                     /protein_id="HEY5049356.1"
                     /translation="LKLLTLPRILGAVEGEEVVASNGRYGPYVKKGSDTRSIDSEEQL
                     LTISLQEALEVLAQPKPRGRRAAAAGPLRELGVDPANQKPVVVRDGRFGPYVTDGETN
                     ATLRKGDDVAAITLERASELLADKRARGPAPKKAAGRRPAKKAATKTVAKTASKTGAK
                     KAAARKTAKKAAAKRT"
BASE COUNT          761 a         1480 c         1341 g          576 t
ORIGIN      
        1 cccatcgagc gaacaccgga caagcggcga cacgaacgcc gcagtccctg cccggccatc
       61 accagcaacc ggcaacaacc accacacctg acctcacacg atcaagatca tgaatgatcg
      121 aggttagctg gcgcgcaggg ctagcgtcag ggcctcgacg gtgagcagtg ggttcgcgtt
      181 cgcgtcgagc gacgtgcggc acgccagcac cgccgacaac cggcgcacgg tcgactctgc
      241 tgacgacgac gctgctaatg ccgagatcgc cgcggtttcg tcggtgttcg cgatgtcgac
      301 gcccgcacct aactgcacca cgagcacgtc gcggtagaaa cccgcgaggt cggttagcgc
      361 acggtccagt gcgtctcgct gcgtgcgcgt ggcgcgggac ttctgccggc cctccagatc
      421 cttcaacgcg gcggcggccg agcgcagcgc gccccgagcc gcacccccgc caccaccagc
      481 agcaccgcga ccgagaccgg tcgccgtccc gccctcgccg aaggcctctt tgtatgacgc
      541 ggtctcggct tcgtcgagcg ccgacgtcac ctcggccgac tccgcaccca cggccttgac
      601 caacgcaccg gcggccgcga ggcatcctcc gacgtccgcc agcgccgacg gcaactgcaa
      661 cacgtcgtgt cgccgcgacc gcgccgcttc atcggtcgcg agccgtcgcg cgcggccgac
      721 gtgaccctgc gccgcccgag ccgcgaactg tgccaccgac ggagaaacac cgtcgcgcaa
      781 aacaagcacc gaagcgaccg catcggtagg tggagtacgc aacgccacca cccggcagcg
      841 cgagcggatc gtcaccacga ggtcttcggc cgaaggcacg cataacagcc acaccgtgcg
      901 cggcgacggc tcctcgatcg cctttaacag cacgttcgca gcgccctcgg tcagccggtc
      961 ggcatcttcg atgatgatga cctgccaatg cccgccggac ggcgcgagcg cggcccgact
     1021 gacgaggtcc cgggcctctc gcacaccgag agataaccct tgcggcgcga cgatttcgac
     1081 gtcggcgtgg gtgccggcga gcaccgtgtg gcagcctaaa caaaccccgc agccgggctc
     1141 gatgtcgcca ccaccattca tcgcggctgt gcattgcaag gcagccgcga atgagcgcgc
     1201 cgcaaccgat cggcccgagc ccggcggacc ggtgaacaac cacgcatgcg tcatcgccgc
     1261 acctggcgct gcagccgcaa ccgcctcggg caacacaacc gatgcgtccg agagcgcacc
     1321 gaacaggtcg ccacccaagt cgctggccca cgcaacttcg gcagccgaga tggccaccga
     1381 acgcaacaac gccaccacgc gctcttggcc gacaaggtcg tcgaagacgc tcaccggcgt
     1441 ccgactttca gttgcactgt cggttgaagg tctggcgaaa gctcgggcac gaaatcggcg
     1501 atccgccgca caatcagctg gtgaatcgtg gccggctgca gcgtcgcgtc gatgacgagg
     1561 tagcggtccg gcgaggcctc cgctagttcg cgaaacgccg ctcgcacccg ctcgtgaaat
     1621 gcggccgact ctgcctccag ccggtccgga agctcgtcgc gaaccgacgt ccgggcaagg
     1681 ccgtctgtga ccgggacgtc gagcagcacc gtcaggtcag gacgcagtcc cgacgtggcg
     1741 aaccgtgaaa gccgctcgat ctcgccggtc gacaacgacc ggccgacgcc ctgatacgcc
     1801 agcgaagaat cgacgtagcg gtcactcacg acgacggcgc cgcgcgcgag tgcaggccgg
     1861 atcaccgtcg ccacgtgctg cgcgcggtcg gccgcgtaca gcaacgcctc ggctcgtggc
     1921 gcgatctcgc cgttgtcggg gttgagcagc aggtgccgca attggcgccc gatcggcgtc
     1981 gcgccgggct cgcgcgtcgt cacgacttcg tacccgcgct cggtcagcca cgacgcgagc
     2041 agcttgcact gcgtcgactt gccggctcct tcgccaccct cgaatgccac gaacagaccc
     2101 gtcgtcacgc cgcggccctc cagcggacgc cagcctcgca gcgcaccgat gaggtcccgc
     2161 cacagcggca catcgcgatc gtccatctgc cggtaggcgg tcacgccgac gacaaaggcg
     2221 atgagtcccg cgcctagcaa ggtgcatgcc gcgccatctt ggcgcaaaac gacccgcttg
     2281 atgtgcaagg tgtgcacgcc gatggccccg gagatgaacg ggccggccac gaccataagg
     2341 agcagatcga cctgcatcaa cgactgcagc gtggcccacg tccggccacg tttctcgtcc
     2401 tcgacctcga ggccgagcaa ggtgtacccc gttacccagg ccacaccaga gaatgcgccc
     2461 atgaccagcg ccccgagcac gacaagcacg aggttgtgga caaccgcggt caaagcgagc
     2521 gtcaaccctg acgcggcaat cgcgacgccg aacagccgcc gtcgcgagag tctccggaag
     2581 aatttcgggc cgaggaacat gccgactgca aggccgacga acaccgaccc gaacatcgcg
     2641 ccatacgcag ggtcaccacc gccgaggtcg agcgtgtatc gctttgccaa cccgaccacc
     2701 acgccggcgg ccgcgaacgc accgagcatg ccgacgacca aaccgcgtac gaccttcgtc
     2761 tgaccaatga actgccagcc ttcagcgatc gcgcgcaaca cgctgggttg cgaaatcggc
     2821 accacgtcgc cggcggcccc gtcctcgagc tcggcgcgcc gccgccgtcc gccctggtga
     2881 gagacctcac gcagggtgaa gatcgtgcct gccgaaacga ggaacgttga ggcgtcgaag
     2941 tacagggcca ggtcgatccg gttggtggtg aagaacggca gcgcgtgacc gagtacgccg
     3001 gtgaacagcg agagcagtgc gaatacgagg gcggcgaccg gtgcagatcc gtatgtcgtc
     3061 agtaggctga gctggttggc ctgctcgagc tgctcacgcg gcaccaggtt cggcacggtg
     3121 gcttccttgg ccggaatcca gaacaggctg agcgtctcga tcaggaacgt cgcaatgaac
     3181 agccaccaca gcgtgtgaac gatcggaatc gagacgtaga gcgagaaccg gccgatgtcg
     3241 gcgatgacca tcgtgagccg tcggtccagc cgatccgcga acgcaccagc gagcggagca
     3301 agcaggacgg cggggagtag ccgtacgaag acgacgagcc ctaccgagga gttctgcacg
     3361 aggtaggtgt gaccggccgc gagctcggcg gccagtgcgg tctgggcaag gaagcccagc
     3421 cagtcaccga agctcgacag cgagagcgct atccacagtt tgcggaacgc cttgatggcg
     3481 agcacaccac gaaagccgcc gacctcggtg gccgcggcgc gggcagcctc gaccagcggc
     3541 gccgggggcg gcaccgcgcc ggggactcgc tcggagatgg ctcaactccc cactgccggg
     3601 aaccgccagt atctcgcgtc ccggcgtcag gtccgcttcg cggcagcctt cttggcggtc
     3661 ttgcgggcgg ccgccttctt ggccccagtc ttgctggcgg tcttggcgac ggtcttggtg
     3721 gcggccttct tggcgggcct gcgccccgcg gccttcttcg gcgccgggcc gcgcgcccgc
     3781 ttgtcagcca gcaactccga cgcgcgttcg agcgtgatgg cggccacgtc gtcgcccttg
     3841 cgaagcgtgg cgttggtctc gccgtcggtc acgtagggcc cgaaacgacc atcgcggacc
     3901 acgacaggct tctgatttgc cggatcgaca ccgagctcac ggagcggacc agcggccgcc
     3961 gccctgcgtc cgcgcggctt gggttgggca agaacctcga gcgcctcctg caggctgatg
     4021 gtcagcagtt gctcctcgct gtcgatcgag cgcgtgtcgg agcccttctt gacgtaaggc
     4081 ccgtagcggc cgttgctggc gacgacctct tcgccttcaa ccgcgccgag gatgcgcgga
     4141 agggtgagca gcttgagg
//
LOCUS       DATCBJ010000026         2145 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_70299, whole genome shotgun sequence.
ACCESSION   DATCBJ010000026 DATCBJ010000000
VERSION     DATCBJ010000026.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2145)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2145)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2145
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_70299"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1174)
                     /locus_tag="VII50_00535"
     CDS             complement(<1..1174)
                     /locus_tag="VII50_00535"
                     /inference="COORDINATES: protein motif:HMM:NF013486.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family N-terminal
                     domain-containing protein"
                     /protein_id="HEY5049357.1"
                     /translation="MSGDWGVALEEIALPEFELPRQQPVIPAGTYQRRVADVYVAMAT
                     RGLDAVIVYGDREHFANMSYLTGFDPRWEESVLILVPGHTPRLVVGNENVTWARVVPY
                     EIDVVRLPKFSLIGQPDPGDVALSDVLRDAGIAAPAVRRVGLIGWKFWTGAATAAWID
                     LPHFIVHEVAALVDSVTNATDMLMSPDGGLRLYNDVDQIAAFEFAGTHASEAVRRLLH
                     GVRAGMTELEATQLMQPILLPFSYHPTMLSGPNAAYGVASAGDRILQVGDPVCTGLGY
                     WGGNTARAGFLVEDVSQLPDAAADYVQKLAEPYYATAAAWYETIRIGLTAGELYDVTF
                     SRIGDPFYGVYLNPGHFIHLDEWPSSPVSPASPVVFTSGNALQLDIIPITGSAYHTA"
     gene            complement(1171..1635)
                     /locus_tag="VII50_00540"
     CDS             complement(1171..1635)
                     /locus_tag="VII50_00540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5049358.1"
                     /translation="MLHDLKTRPQAALAHLNRRQQTALTSWSAFTATFVAVRGITYAI
                     KDNVGPFHNVTLGGEHIHHYMWGIGMVATAGGIALQDQDTKDPHTALAIVYGSGLALI
                     VDEFAELLDLQDVYWAKQGRVSVDIAIAVIATGGSYLAARPFWHRLVKGRTR"
     gene            complement(1640..>2145)
                     /locus_tag="VII50_00545"
     CDS             complement(1640..>2145)
                     /locus_tag="VII50_00545"
                     /inference="COORDINATES: protein motif:HMM:NF024770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEY5049359.1"
                     /translation="GADSVAAGNGDPDAPARVPGVQLGMRARVSGFVRALYAAGIDPA
                     SVSVVAGARNSREAGRDAAARMLADRHPTAVFGVTDVLALGVLDAAASRGVEVPRQLS
                     VVGFDDVAAAAASVPPLTTVAQSLFDQGRLAASVVLDQVAGRPVHIPPIRTEVVVRGS
                     TAPPLYN"
BASE COUNT          363 a          726 c          716 g          340 t
ORIGIN      
        1 gtgccgtgtg atacgcgctg ccggtaatcg ggatgatgtc cagctgcaac gcgttgccgg
       61 acgtgaatac cacgggagat gcggggctga ccggagacga cggccactcg tcgaggtgga
      121 tgaagtggcc cggattgaga tacacgccgt agaacggatc gccgatccgg ctaaacgtca
      181 cgtcatagag ctcccccgcc gtcaagccga tgcggatcgt ctcgtaccag gcggccgcgg
      241 tcgcgtagta cggctccgcg agcttctgca cataatcggc ggcggcgtcc gggagctggc
      301 tcacgtcttc gacgaggaac cccgcccgag cggtgttgcc gccccagtag ccaagtccgg
      361 tgcagaccgg gtcgccgacc tgcagaatgc ggtcacccgc gctcgccacg ccgtacgccg
      421 cgttcggccc gctgagcatc gtcgggtgat agctgaaggg cagcaggatg ggctgcatca
      481 gctgcgtcgc ctcgagttcg gtcattccag cccggacgcc gtgcagtagc cgccgtaccg
      541 cctccgaggc gtgagtgccg gcaaactcga acgccgcgat ctgatcgacg tcgttgtaga
      601 ggcgcaaacc gccgtccggg ctcatcagca tgtccgttgc gttcgtgacg ctgtcgacga
      661 gcgccgcgac ctcgtgcacg atgaagtgcg gcaggtcgat ccacgcagcg gtggcggcgc
      721 ccgtccagaa cttccaacca atcaagccaa cccggcggac ggcgggagcc gcgatcccgg
      781 cgtcgcgcag gacgtcggac agcgccacgt cgccgggatc cggctggccg atcaggctga
      841 acttcggcag ccggacaaca tcgatctcat acggcaccac cctggcccac gtgacgtttt
      901 cgttgccgac gacaagccgc ggggtatggc cggggaccag gatcagcacc gactcctccc
      961 agcgcgggtc gaagcccgtc aggtaggaca tgttggcgaa gtgctcgcgg tcgccataga
     1021 cgatgacggc gtcgagaccg cgcgtcgcca ttgccacgta gacgtccgcg acgcggcgct
     1081 ggtaggtccc cgccgggatc accggctgct gccgtggcag ttcgaactcc ggaagggcga
     1141 tctcttcgag cgccacgccc caatcgccgc tcatcgcgtt ctccctttga cgaggcggtg
     1201 ccagaacggg cgggccgcca gataggagcc accggttgcg atcaccgcga tcgcgatatc
     1261 gacgctgacg cgaccctgct tcgcccaata cacatcctgc aggtcgagca gctcggcgaa
     1321 ctcgtcgacg atcagcgcca gccctgagcc gtacacgata gccagtgcgg tgtgcggatc
     1381 cttggtgtct tgatcctgca acgcgatccc gccggcggtg gccaccatgc cgatgcccca
     1441 catgtagtgg tgaatgtgct cgccaccgag tgtcacgttg tggaatggcc cgacgttgtc
     1501 cttgatggcg taggtgatgc cacgcacggc gacgaatgtt gccgtgaacg cgctccagct
     1561 cgtcagtgcc gtctgttggc gccggttcag gtgggccaaa gccgcctgcg gtcgagtctt
     1621 caggtcgtgg agcatggcgt cagttataca gcggcggcgc ggtgctgcct cggacgacga
     1681 cttcggtgcg gatcggcggg atgtgcaccg ggcgccctgc gacctgatcg agcaccacgc
     1741 tcgcggccag ccgaccctga tcgaacagcg attgcgccac cgtggtcagt ggcggcacgc
     1801 tcgcagcagc ggccgcgacg tcatcgaacc ccacgaccga cagctgccgc gggacctcga
     1861 cacccctgct cgcggcggca tccaggacgc cgagtgcgag cacgtcggtg accccgaata
     1921 ccgcggtcgg atgccgatcc gcgagcatcc gcgcagcagc gtccctacct gcctctcgcg
     1981 agtttcgggc cccggcgacg accgacacgg aagccgggtc gattccggcc gcatagagcg
     2041 cccgcacgaa tccggaaact cgagctcgca tgccgagctg gacgccgggc acgcgtgctg
     2101 gtgcgtcggg gtccccgttg ccggcggcga ctgagtcggc accat
//
LOCUS       DATCBJ010000027         3454 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_364142, whole genome shotgun sequence.
ACCESSION   DATCBJ010000027 DATCBJ010000000
VERSION     DATCBJ010000027.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 3454)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3454)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3454
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_364142"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1178)
                     /locus_tag="VII50_00550"
     CDS             complement(<1..1178)
                     /locus_tag="VII50_00550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009073038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HEY5049360.1"
                     /translation="MSLTRRTALIALAGVLVVFVYPLRGYMVLIVEGALAIGIIADLA
                     LAGSVRALRVSRYGDTARRLDEVSEVGVLIVNPGGRRVRGVLRDAWPPSAQASPTRQL
                     IDVPAGQRRRFPTAIRPRRRTELQGGRVTVRSFGPLGLAARQGNHDVPWSLRVLPAFP
                     SRRYLPEKLAKLRILDGRTAARVRGQGTEFDSLRDYVEGDDARSIDWRATARRSAVVV
                     RTWRPERDRRIVLVLDTGRTSAARIGAAPRLDAALDAALLLGTLASRAGDRVDLIAVD
                     DRPRLLSRGARDHGDPLSGLLEAMAGLHPRLVETDGRRLVAEVLRRVKQRALIVLFTT
                     LDEAAITEGLLPVLPSLTRKHVVMVASVADPQVLAMVGVHDDTRTVYDAAAAERTTAA
                     "
     gene            complement(1186..2154)
                     /locus_tag="VII50_00555"
     CDS             complement(1186..2154)
                     /locus_tag="VII50_00555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016461382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HEY5049361.1"
                     /translation="MTSTSSIEQSRDAFAAVRAEVAKAVVGQDAVVSGLVIALLADGH
                     VLLEGVPGVAKTLLVRSLAAALSLDTKRVQFTPDLMPGDVTGSLIYDARTAEFQFRKG
                     PVFTNLLLADEINRTPPKTQAALLEAMEERQVSVDGLPRPLPDPFMVIATQNPVEYEG
                     TYPLPEAQLDRFLVKLNVPLPGRDDEFGMLRRHAEGFDPRDLAAAGLRAVAGPTELAA
                     GRAAAARVSVAPEVLSYIVDLCRATRSSPSVSLGASPRGATALLRTSRAWAWLSGRDF
                     VTPDDVKALARPTLRHRLALRPEAELEGATTDAVLEGVLATVPVPR"
     gene            complement(2151..3341)
                     /locus_tag="VII50_00560"
     CDS             complement(2151..3341)
                     /locus_tag="VII50_00560"
                     /inference="COORDINATES: protein motif:HMM:NF025617.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4350 domain-containing protein"
                     /protein_id="HEY5049362.1"
                     /translation="MTVLEELPGATDGGPIRVGHSADPTIRERWRRSRVLIGAGVVVV
                     LGAIIIALGSGTGGAALDPRSYAPSGTHALSILLGQRGVHVTTETDVATAEQLAVQDT
                     TLVVVSPQLLSADELSAIASSHADLVVVGAGLAEIQGLGLPLATQPDDGNTLRQPSCS
                     LPAAMVAGSAQLTGGGYTVPNAGQGCYPDGDGFALVTLERSGHRVTLLSDGTPLMNSS
                     LANDGNAALALGLLDIHPDVVWLVPLRIAPASSGGGNASVPSLLPSRLKLAFLQLVIA
                     VIVIAIWRGRRLGRLVPEDLPVVVRQSETVRGRSRLYRRSRSLDTAATALREGTRDRL
                     GHRLGLGLRPLPAALVQAVAARAGQPSVQVEALLYGSTPADDLTLVTLAQALTALEQE
                     VLRT"
BASE COUNT          606 a         1205 c         1137 g          506 t
ORIGIN      
        1 tgcgcagccg tcgtccgctc tgccgcggcc gcgtcataca ccgtgcgcgt gtcgtcgtgc
       61 acgcccacca tcgcgagtac ttgcgggtcg gccaccgagg cgaccatcac cacgtgcttg
      121 cgggtcagcg acggcaagac aggcagcagt ccctcggtga tcgccgcttc gtccagtgtc
      181 gtaaacagca caatgagcgc acgctgcttc acccgtcgca gcacttcggc caccaaccgg
      241 cgcccatctg tttcgaccag gcgagggtgc aacccggcca tcgcctcgag caggccagaa
      301 agtggatcgc cgtgatcgcg cgcaccgcgc gagagcagcc gcggccgatc atccaccgcg
      361 atcagatcga ctcggtcgcc cgcacgcgac gcgagcgttc caaggagcaa tgccgcgtcg
      421 agcgcagcgt cgagtcgcgg tgctgcgccg attcgagccg ccgacgtgcg gccggtgtcg
      481 aggacgagta cgatccgccg gtcgcgttcc ggccgccagg tgcggacgac gactgctgac
      541 cgccgagcgg ttgcgcgcca gtcgatcgac ctggcgtcgt cgccctcgac gtagtcacgc
      601 aacgagtcga actcggtgcc ctgtccgcgg acccgcgcag ctgtgcggcc gtcgagaatc
      661 cgcagcttgg caagcttttc cggcaggtat cgccggctcg gaaacgcagg caacacccgc
      721 aaactccacg gcacatcgtg attgccttgc cgcgcagcga gaccgagcgg cccgaagctg
      781 cgcaccgtga ctcggccgcc ctggagttcg gttcggcgtc gcggccggat cgccgtcggg
      841 aagcgacgcc gttgacccgc ggggacgtcg atcaactggc gagtaggtga cgcttgcgca
      901 ctcggcggcc atgcatcgcg caggacgccg cgcactcggc gtccgcccgg attgacgatg
      961 agcactccca cctcggacac ctcgtcgagg cggcgcgcag tgtctccata tcgagacacc
     1021 cggagcgcgc gcacgctgcc cgccaacgca aggtcggcga tgatgccgat cgccaatgcc
     1081 ccttcgacga tcaataccat gtagccgcgc agcggataca cgaacaccac cagcacgccg
     1141 gcaagcgcga tgagtgcagt gcgccgcgtc agtgacacga aacactcacc ttggaacggg
     1201 gaccgtggcg agcacgcctt cgagcaccgc gtcggtggtc gcaccttcga gttcggcctc
     1261 ggggcgcaac gccagccggt ggcgcaacgt cggtcgcgcc aaagccttca cgtcatcagg
     1321 agtcacgaag tcgcggccgg acaaccacgc ccacgcacgc gacgtccgca gcaacgctgt
     1381 agcaccacga ggcgacgcac caagtgacac cgacggtgaa ctgcgcgttg cccggcacaa
     1441 gtcgacgatg tagctcaaca cctccggagc taccgatacc cgtgccgctg cagctcggcc
     1501 cgcagccagc tcggtaggcc cagcgactgc acgcaaccct gctgccgcaa gatcgcgtgg
     1561 atcgaatcct tcggcgtggc gtcgcagcat gccgaactca tcgtcgcgac cgggcagcgg
     1621 aacgttgagc ttcacgagga accggtcgag ctgtgcctcg ggcaacgggt aggtgccttc
     1681 gtactcgacc gggttctgtg tcgcgatcac catgaacgga tcaggcagcg gccgcggtag
     1741 tccgtcgacc gacacctgtc gttcctccat cgcttcgagc aatgcggcct gcgttttagg
     1801 cggtgtgcgg ttgatctcgt ccgcgagcag caggttcgtg aacaccgggc ccttccggaa
     1861 ctggaactcc gcggtgcgcg cgtcgtagat gagcgagccg gtcacgtcac cgggcatcag
     1921 gtcgggagtg aactgcaccc gcttggtgtc gagtgacagt gcagcggcga gcgaccgcac
     1981 gagcaacgtc tttgccaccc cgggcacccc ttcgagcagc acgtgcccgt ccgcgagcaa
     2041 cgcgatgacg agaccgctca cgacggcgtc ctgcccgacc acggccttgg cgacctcggc
     2101 ccgcaccgcg gcgaacgcgt ctcgcgactg ctcgatggaa ctggtcgacg tcacgtccgg
     2161 agtacctcct gctcgagggc ggtcagtgct tgcgccagcg tcaccagtgt gaggtcgtcg
     2221 gccggtgtcg aaccgtatag gagcgcctcg acctgcacag aaggctgtcc tgctcgtgca
     2281 gcaacggcct ggacgagcgc cgcgggaagt ggtcgaagac cgaggcccag ccggtgtcca
     2341 aggcgatcgc gcgttccttc gcgtaacgcc gtagccgcgg tgtcgagcga gcgtgatcgc
     2401 cgatacagac gggagcggcc gcgcacggtc tcggattgcc ggacgacgac cggcaggtct
     2461 tctggaacca gccgacccag gcgtcgaccg cgccaaatcg cgatgacgat gaccgcgatc
     2521 accagttgca ggaacgccag cttcaaccgg ctcggcagca gcgatgggac actcgcgttt
     2581 cctccgccgc tcgatgcggg agcgatccgc agcggcacca gccacacgac gtccggatgg
     2641 atatcgagaa gccctagggc aagtgcggcg ttgccgtcgt tggcaagcga cgagttcatc
     2701 aggggagtgc cgtcggagag caacgtcacg cgatggcccg atctctcgag ggtcaccagc
     2761 gcgaagccgt cgccgtcggg atagcagcct tgcccggcgt tcggcacggt gtagccgccg
     2821 ccggtcagtt gagccgaacc cgccaccatc gcggccggca aggaacacga gggttgccgc
     2881 agcgtgttcc cgtcgtcggg ttgggtcgcc agcggcaggc cgagcccttg gatctcagcc
     2941 agccccgcgc cgacgacgac gagatcggca tgcgacgacg cgatcgcgct cagttcgtct
     3001 gcactcagca actgaggcga gacgaccacg agcgtcgtgt cttggacggc gagttgttcg
     3061 gcggtggcga cgtccgtctc cgtcgtgacg tgcacgccgc gttgcccgag caagatcgaa
     3121 agtgcgtgag tgcccgacgg cgcgtaggac cgcggatcga gcgcggcgcc acctgtcccc
     3181 gaacccagcg caatgatgat tgcgccgagc acaaccacga ccccggcacc gatcagcaca
     3241 cgtgagcgcc gccaacgctc gcggatcgtg gggtcggcgc tgtgccccac gcggatcgga
     3301 ccgccgtccg tcgctcccgg caactcctcg aggacggtca cgtgctgcca accagaccgc
     3361 gatgggagcc ggcgacgcga gcgtcgagcc tacgcagcaa ctcctcgtcg gcggcgctcg
     3421 ccgtgcggcc tccgtaccag acctcgtcga agat
//
LOCUS       DATCBJ010000028         2829 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_547985, whole genome shotgun sequence.
ACCESSION   DATCBJ010000028 DATCBJ010000000
VERSION     DATCBJ010000028.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2829)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2829)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2829
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_547985"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..563)
                     /locus_tag="VII50_00565"
     CDS             complement(<1..563)
                     /locus_tag="VII50_00565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5049363.1"
                     /translation="MAVSEQIILVGSRSNVGKVTIDPAAIRSEGGIRPELIVPITVVM
                     NARTADGQLAVTWLRGRLRTSFHVAPGSEICAPADYSLLANMPARSLPSSAANDHRVD
                     LRFAIGPAETAALDSLRHVGGASSGLDLYLSLDVHVAGLATAGEVSAPHPSAQTPTSQ
                     WGTEFGLHSWLAYFWSVSIQPLTIAISR"
     gene            complement(765..1397)
                     /locus_tag="VII50_00570"
     CDS             complement(765..1397)
                     /locus_tag="VII50_00570"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEY5049364.1"
                     /translation="MRVIVADDQTAVREGLVTLLSTMPGIDVVGSAADGEEAITLVAQ
                     LTPDVVLMDLRMPHVDGIEATRRIRAAHPTTQVVVLTTYADDESILAALRAGAIGYLT
                     KDAARDHIRRAIEAAAAGQAVLDPAVQARLVSVAQPPRSDSSQLPDGLSEREGEVLRL
                     IASGKSNSEIAAQLFVSEATIKTHVNHIYAKTSSRDRAQAVTYAHQHRLT"
     gene            complement(1394..2539)
                     /locus_tag="VII50_00575"
     CDS             complement(1394..2539)
                     /locus_tag="VII50_00575"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HEY5049365.1"
                     /translation="MRPSQRLRASGSDPVGGAVRAVRIVGLGVLLWMVASEHHSDGSP
                     RVPAALLAITAAGWITWLVCSHLNRPKRETWLALGVVAGVGGVVGGLAPLGITFPAVS
                     VIATASLIGVRQALTVAALGAVALTTTVLAARTPNAIIAEGLLALAAAILGGASRRQY
                     QERVEQAEQLLAERQRGDAEHARAAALAERNRIGREVHDVLAHSLGALSVQLEAADAL
                     LETDGDIPSVRKHVQQARRLAVDGLVETRRAVHALRDEPGELRAQIATLASAAGAPLS
                     VTGAERRLDADVELALYRAAQEALSNARKHAPGAAVTLRLDYGPTATVLVVENGPRPS
                     ASSPSPLTATGGGFGLRGMRERIELLGGDVSAAAQDSGWTVRATVPV"
     gene            complement(2577..>2829)
                     /locus_tag="VII50_00580"
     CDS             complement(2577..>2829)
                     /locus_tag="VII50_00580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5049366.1"
                     /translation="AVLWVAGIGARVAFSMWTSHGGSATLRDFSIAHHITSGTAWGAA
                     FILMAIAEVATRTGVIFVKTVRTGAVIERGGLIRRFVTA"
BASE COUNT          453 a          929 c          986 g          461 t
ORIGIN      
        1 cggctgatgg caatcgtgag cggctggatg ctcacgctcc agaaatacgc gagccacgaa
       61 tgcaggccga actcggtacc ccactggctg gtcggagtct gcgccgacgg atgcggtgcg
      121 gatacctcgc ccgcggtcgc cagaccggcc acatgtacgt cgagagacaa gtacaggtcg
      181 agaccgctac tggcgcctcc gacgtggcgg aggctgtcga gggcggccgt ttcggctggg
      241 ccgattgcaa accgcagatc aacccggtgg tcatttgcag cgctcgacgg taaggaccgt
      301 gcgggcatgt tggcaaggag tgagtaatcg gcaggcgcac aaatctcgga gcccggagcc
      361 acatggaagc tggtccgcag gcgaccgcgt agccaggtca cggccaactg gccgtcagcc
      421 gttcgggcgt tcatcacgac cgtgattggc acgatcagct ccggtcggat accgccttca
      481 ctgcggatcg ccgccgggtc gatcgtgacc ttcccgacgt tgctccgaga cccgacgagg
      541 atgatctgct ccgaaactgc cattcgacaa ggctatggcg caccgtgact gagagcgatt
      601 tctttgtccc gacacgtcct cgcgccggtg tcgtgacgcg ctcgtgggcg ggttcggacg
      661 cgcgtcatcg cggcagaagc gtggacatcc cggcagatgc ggacgttgct gggttcagct
      721 tgggactact gccgccctca accttggcga ccacccggag gcggtcacgt tagtcggtgt
      781 tggtgggcgt aagtgaccgc ctgtgctcga tcgcggctgc tggtcttggc gtagatgtga
      841 ttgacgtggg tcttgattgt cgcctcgctg acgaacagct gtgccgcaat ttcgctgttc
      901 gacttcccgg aggcgatcag gcgcaagact tcaccttcgc gttcggagag tccgtctggc
      961 agttgtgacg agtcggaccg cggcggttgg gcgaccgaca cgaggcgtgc ttgcaccgcg
     1021 gggtcgagca cggcttgtcc agcggcggca gcctcgatcg cgcggcggat gtggtcgcgg
     1081 gcggcgtcct tggtgaggta tccgatcgcg ccggcgcgca gcgcagcgag gatcgattcg
     1141 tcgtctgcgt aggtggtcag gacgacgact tgtgtcgtgg ggtgcgccgc gcggatgcgc
     1201 ctggtcgctt cgatcccgtc gacgtgaggc atccgcaagt ccatcagcac cacatcgggt
     1261 gttagctgcg caacgagggt gattgcttct tcgccatcag cggccgatcc gacaacgtcg
     1321 atgcccggca tcgtcgacag caacgtgacg aggccttctc gaacggcggt ctgatcatcg
     1381 gcgacgatga ctctcatacc ggcaccgtcg cgcgcacggt ccagccggag tcctgcgcag
     1441 cggcggagac gtcgccgccg aggagttcga tgcgttcgcg catgccgcga agtccgaagc
     1501 ctccgccggt ggcagtcagt gggctggggg agctcgcgct cggacggggc ccgttctcga
     1561 cgacgaggac tgtggcggtc gggccgtaat cgagccgcag cgtgacagct gccccgggtg
     1621 cgtgcttgcg ggcattgctc aacgcttcct gcgccgcgcg gtacagcgcg agctcgacat
     1681 cggcatccag tcggcgctca gcaccggtca cactcagtgg cgcaccggct gccgaagcga
     1741 gtgtggcgat ctgcgcgcgc agttcgcccg gctcgtcgcg cagcgcgtgc acagccctgc
     1801 gtgtctcgac caggccgtcg acggcgaggc ggcgggcctg ttggacgtgc ttgcgcacgc
     1861 ttggtatgtc gccgtcggtc tcaagcaacg cgtcggcggc ttcgagttgc accgacagcg
     1921 cgcccagaga gtgcgctagt acgtcatgca cctcgcgtcc gatgcggttg cgctcagcca
     1981 gtgccgcggc gcgggcgtgc tcggcgtcgc cgcgctggcg ctccgcgaga agctgctcgg
     2041 cctgctcgac gcgctcttgg tactgccgac ggctcgcgcc acccaggatc gctgcggcga
     2101 gggccagcag cccctcagcg atgatcgcgt tcggcgtccg ggcggcgagc accgtcgtgg
     2161 tcagggccac ggcaccgagc gcggcaacgg tgagggcctg ccgcaccccg ataagcgaag
     2221 cagtcgcgat gaccgagact gccgggaagg tgatgcccag cggcgccagc ccgccgacga
     2281 cgcctccgac ccccgccacc accccaaggg cgagccacgt ctcgcgcttg ggtcggttga
     2341 ggtgactgca gaccagccag gtgatccagc ccgccgcggt gatagccagt agcgccgccg
     2401 gaacacgtgg cgagccatcg gagtggtgtt cgcttgcgac catccacagc aacacgccca
     2461 acccgacgat gcggacggcg cggacggcgc ctccaaccgg gtcagacccg gaagcccgaa
     2521 gccgctgcga aggcctcatg ccggtcatcg tctcaccgcc gcctggcctg agggggctac
     2581 gcggtgacga accggcggat caggccgccg cgctcgatca cagcgccggt gcgcaccgtc
     2641 ttgacgaaga tgacgccggt ccgggtcgca acttcggcga tcgccatcaa gatgaaagcg
     2701 gcaccccagg cggtgcccga cgtgatgtgg tgggcgatgc tgaaatcgcg gagcgtcgcc
     2761 gagccgccgt ggctcgtcca catcgagaac gcgacccgag caccgattcc cgcgacccac
     2821 agcacggca
//
LOCUS       DATCBJ010000029         2242 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_400953, whole genome shotgun sequence.
ACCESSION   DATCBJ010000029 DATCBJ010000000
VERSION     DATCBJ010000029.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2242)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2242)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2242
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_400953"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..985
                     /locus_tag="VII50_00585"
     CDS             <1..985
                     /locus_tag="VII50_00585"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEY5049367.1"
                     /translation="VALCEPVRDAGGYYCPHHRNYGRSVVQGYQDCLSIAKCADVALH
                     LAHCHVNYLQNAGRAIEVLDAIDAAVAQDGLDVSLDSYPYLAGATYLAALLPSWVHAG
                     GADAVVSRLQDPETRRRILYEIEVSGSDGSHNMPVDWTTISVSGVADERSAWAVGATI
                     AELASARDEEPGALFCNLLVADRLGVGCLVHVGNEDNVRAIMRHRAHTAGSDGILVGA
                     RPHPRGWGTFPRYLGTYVRELGILRLEECVAHLTSRPARRLRMVDRGVLRVGAVADIV
                     VFDADTIDATGTYDDPRRQPQGIQHVMVNGEYTLRDGHRTDRLPGRAIRRA"
     gene            complement(1014..1886)
                     /locus_tag="VII50_00590"
     CDS             complement(1014..1886)
                     /locus_tag="VII50_00590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009084399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEY5049368.1"
                     /translation="MQDEQFWAERFEQQRPRLQALAYRMLGSTAEAEDAVQETWLRVS
                     RASGEDVANAAGWLTTIVSRQCLNMLRSRAARREDPLDIRMPDPVVEFGDGVDPAEQS
                     VLADSVGLALLVVLESLDPAERLAYVLHDMFAVPFDDIAPMLERTPAATRQLASRARR
                     HIQGATATGRPDRVRQREIVDAWLAAARSGDFAGLVAMLHPNAVLRVDTGRADSKLVR
                     GAAAIAGQAMRYRATAALLAHFATVNAGPGIIGTVDGRVTSVLGFTVANGLIVEVNIL
                     TDPRRLEALDLGLS"
     gene            1945..>2242
                     /locus_tag="VII50_00595"
     CDS             1945..>2242
                     /locus_tag="VII50_00595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009084398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEY5049369.1"
                     /translation="MTAVAMEEMMEARMTHPVMVLPDAMSALLALTNAAQSGTIPPAT
                     HQLIQLRVSQINGCSVCVDMHARELKNLGEKDERIWGVGAWRESPLFSEAERAAL"
BASE COUNT          388 a          719 c          750 g          385 t
ORIGIN      
        1 cgttgcgctc tgcgagccgg tgcgtgatgc cggcggctac tactgcccgc atcatcgcaa
       61 ttacggtcgc tcggtggtcc agggttatca ggactgtctg agcatcgcga aatgtgcgga
      121 cgttgccctg cacctcgcgc attgccacgt gaattacctg cagaacgccg gtcgggcaat
      181 cgaggtactt gacgccattg acgcggccgt cgcacaggat ggtctcgacg tcagcctcga
      241 cagctatccc taccttgctg gcgcgactta cctggccgcg ttgttgccga gctgggtgca
      301 tgctggcggc gctgacgccg ttgtctctcg gctgcaagat ccggagacgc gccgccgaat
      361 cctctatgaa atagaggttt ccggctcgga cggatcgcac aacatgccag tcgactggac
      421 cacgatctct gtatcggggg ttgctgacga gcgatcggcg tgggcggtcg gcgcgacgat
      481 cgccgaactc gcgagtgctc gcgatgagga gcccggagcg ttgttttgca acctgctcgt
      541 cgctgatcgg ttgggtgtcg gctgcctcgt gcatgtgggc aacgaggaca acgtgcgcgc
      601 catcatgcga cacagggcac atacggcggg cagtgacggc atcctcgtcg gcgcgcgacc
      661 acatccgcgc ggttggggaa catttccgcg ctacctaggc acgtacgtgc gtgagctcgg
      721 cattctcagg ctcgaggaat gtgtcgccca cctgacgtcg cgcccggccc ggcgcctgcg
      781 aatggtcgac cgcggggtgc tacgagttgg tgccgtggcc gacatcgtcg ttttcgacgc
      841 cgacacgatc gatgcgacag ggacgtacga cgacccgcgc cgccagccac aggggattca
      901 gcacgtgatg gtaaacggcg agtacacgtt gcgcgatggc caccgcaccg accgcctgcc
      961 tgggcgggca atcaggcgcg cctagagttc gcggtcgtcg caggggtcgc gtgtcaacta
     1021 agcccaaggt cgagggcctc gagtcgtcgc gggtcggtca agatgttgac ctcgacgatg
     1081 agcccattgg cgacggtgaa gccaaggact gacgtcacgc ggccatcgac tgttccgatg
     1141 atgccggggc cggcgttgac ggtggcgaag tgcgcgagga gtgctgcggt cgcccggtat
     1201 ctcatcgcct gcccggcgat ggcggccgca ccgcggacaa gcttggagtc ggcgcggccg
     1261 gtgtcgacgc gcagcaccgc attcgggtgc agcatggcga ccaaccctgc gaagtcgccg
     1321 ctgcgtgcgg cggcgagcca ggcgtcgacg atctcccgct gtcgcacgcg gtcgggtctt
     1381 ccggtcgcag tcgcaccctg gatgtggcgg cgtgcccggc tggctagctg tcgcgttgct
     1441 gccggggttc gctccagcat cggggcgatg tcatcgaagg gcacggcgaa catgtcgtgt
     1501 agcacgtaag cgagtcgctc ggcaggatcc agcgactcaa gcacgaccag cagcgccaga
     1561 ccgaccgagt cggcgaggac gctttgctcg gccggatcga ccccatcacc gaactcgacg
     1621 actgggtcgg gcattcgaat atccagcggg tcctcgcgcc gggccgcccg tgagcgcagc
     1681 atgttcaggc attgccgcga cacgatcgtc gtgagccaac cagcggcgtt ggccacgtcc
     1741 tcgcctgagg cacgggagac gcgcagccag gtttcctgaa cagcgtcctc ggcctcagcc
     1801 gtcgagccca acatccgata ggcgagcgcc tgcaaccgcg gtcgctgctg ctcgaaacgc
     1861 tcagcccaga actgttcgtc ctgcacgtgt cacatcctct cgacccggcg ggtcacttct
     1921 atgacacgcc ccagcagggc cgatgtgacc gcagtagcga tggaggaaat gatggaagcc
     1981 cgaatgactc accctgtcat ggtgctgccc gacgcgatga gcgcgctgct cgcgcttacc
     2041 aacgcagcgc agtcaggcac tatcccgccc gcgacgcacc agctgatcca gcttcgagtc
     2101 agtcagatca acggctgcag cgtgtgcgtc gacatgcacg ccagagaact caagaacctc
     2161 ggtgagaaag acgaacggat ctggggcgtc ggagcatggc gggaatcgcc gctcttcagc
     2221 gaagccgaac gggccgctct cg
//
LOCUS       DATCBJ010000030         3934 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_69823, whole genome shotgun sequence.
ACCESSION   DATCBJ010000030 DATCBJ010000000
VERSION     DATCBJ010000030.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 3934)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3934)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3934
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_69823"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(12..296)
                     /locus_tag="VII50_00600"
     CDS             complement(12..296)
                     /locus_tag="VII50_00600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5049370.1"
                     /translation="MEDDSMDDTVGEDVEADAGIETDAGIETDVDSEVDADATELATW
                     TRPEVTGVASVDAAIEELARLDQLPTGEHVAVYEGLHRQLQDALADLDGA"
     gene            384..716
                     /locus_tag="VII50_00605"
     CDS             384..716
                     /locus_tag="VII50_00605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5049371.1"
                     /translation="MASPDECKAAIAQLAARLAGPGDRRAEGLDRSVSAEVTDLGLVF
                     RGHLHDGVLDDITTDDAPPAQIRLILSSDDLVLLAVGELSLGSAWLSGRVKLQASLTD
                     MLRLRTMI"
     gene            complement(737..1777)
                     /locus_tag="VII50_00610"
     CDS             complement(737..1777)
                     /locus_tag="VII50_00610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIA family hydrolase"
                     /protein_id="HEY5049372.1"
                     /translation="MTRAAGRSLLGISTPLATTYDVALLDLDGVVYLGPDPIPSAAPA
                     LAKARAAGMRLAFVTNNASREPAAVAAHLVALGVPAEAGDVVTSSQAAATVLVERLGV
                     GASVLVTGSAALRTAVELVGLRVVTSSDDRPDAVVQGYSPELRYADLAEAALAVRAGA
                     LWVASNTDSTLPSPRGLLPGNGSLVAAVAVATGRRPIVAGKPQLPMHAEGVRRTDAKN
                     PLVVGDRLDTDIEGANVADTDSLLVLTGVTAATDLVDASVEHRPTFLAADLTGLVDTQ
                     PNVERDGDMTRCGGWTASADRLVGRIAISIHGDGRPIDGLRALLTAAWATRDADAAAV
                     DASAALRRLAFS"
     gene            complement(1774..2172)
                     /locus_tag="VII50_00615"
     CDS             complement(1774..2172)
                     /locus_tag="VII50_00615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY5049373.1"
                     /translation="MDEPSPSVDGEGPYDWYTRGLALLEAGSAGAAEQLLARAATAEP
                     DSRSVLEALGRAQFEARHYAKAKATFSRIIEADPTDDYGQFALGLSAARIGELRTAVE
                     HLALAAAMRPDLAHYGRELRMVRARLEGAT"
     gene            2265..3548
                     /locus_tag="VII50_00620"
     CDS             2265..3548
                     /locus_tag="VII50_00620"
                     /inference="COORDINATES: protein motif:HMM:NF018004.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1015 domain-containing protein"
                     /protein_id="HEY5049374.1"
                     /translation="MTASPTGTLQLLPFRAVRYNTSVSDLAAVLAPPYDVIDDAGQAA
                     LEATDPHNVVRLILPRDPAIPGLDRYAAAAQTFGQWLDDETLLVDDAPALYVYQQTRA
                     GAVLQRGLLGAVVMQPIDGGVILPHENVRSGPVEDRLALMRAMRANPEPIFLLYDGGG
                     AATASSASAVARDPVASTRTSDGIKHELWSITDVAELAGIAADLAGRHAMIADGHHRY
                     TTYGYLRDELHAAGEGAGPWDAGLTLLVDASVAAPDIRAIHRVVPQLPLDEALIAAAD
                     AFRIVRLNADVDGVDRALALLSEHDSPAFLLGDGRSFALLLDPDPDRLAAAKPSDRAP
                     AWWALDASIASVFVMESLFGVSDAAGQVEAEHDPAAALRLAQRDNGIALFLKPAPLAG
                     IVAVAEAGDAMPRKSTLFLPKPRSGMAMRAFRFEG"
     gene            complement(3553..3867)
                     /locus_tag="VII50_00625"
                     /pseudo
     CDS             complement(3553..3867)
                     /locus_tag="VII50_00625"
                     /inference="COORDINATES: protein motif:HMM:NF012651.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          631 a         1398 c         1257 g          648 t
ORIGIN      
        1 cccgccgcgg ctcaggcgcc atcgagatcg gcaagcgcat cctggagctg gcgatgcagg
       61 ccctcgtaga ccgcgacgtg ctcgccggtc ggaagctgat ccagtcgggc gagttcctcg
      121 attgccgcgt ccacgctcgc cacgccggtg acctcgggtc gcgtccacgt cgccagttcc
      181 gttgcgtcgg cgtccacctc gctgtcgacg tcggtctcga tgccggcgtc ggtctcgatg
      241 ccggcgtcgg cttcgacgtc ctcgcccacg gtgtcgtcca tgctgtcgtc ttccacgcga
      301 ccacggtacc ggtcgacgcg ttgcggctga ggtagcctcg gcgcggcccc atcatccaac
      361 cgccccggcc ggagaggacg cgcgtggcca gccccgacga atgcaaggca gcgatcgccc
      421 agctagctgc tcgactggcc ggtcctggtg atcgccgtgc cgagggactc gaccgctcgg
      481 tcagcgcgga ggtcacggac ctcggcctgg tatttcgcgg tcatttgcac gacggcgttc
      541 tcgatgacat cacgacagat gacgcaccac ccgcccagat ccgactgatc ctcagcagcg
      601 acgacctggt cctactcgcc gtcggcgagt tgtcgctggg gtcggcgtgg ctgtcgggtc
      661 gggtcaagct gcaagcctcc ctgaccgaca tgctgcgtct gcgaacgatg atctgaacgg
      721 cgtcccagcg gggcgatcag gaaaaggcga gcctccgcag agcagctgag gcatccaccg
      781 cagcagcgtc cgcgtcgcgt gtcgcccacg ccgccgtcag cagcgcacgc agcccatcaa
      841 tcggacgacc gtcaccatgg atgctgatcg cgatgcggcc gaccagccgg tcagccgacg
      901 ccgtccagcc accgcatcgc gtcatatcgc catcgcgttc gacgttgggt tgggtgtcga
      961 ccagccccgt caggtcggct gcgaggaacg tcgggcggtg ctcaacggat gcatcgacca
     1021 agtcggttgc agcggtcacg ccggtcaaaa ccagcagact gtcggtgtct gcaacattcg
     1081 caccctcgat gtcggtatcg agtcggtcgc cgaccacgag cggattcttc gcgtcagtac
     1141 gccgaacacc ctccgcgtgc atcggtagct gcggcttgcc tgccacgatc ggtcgccggc
     1201 cggtcgccac cgcgaccgct gcgaccaacg acccattccc tggcagcaag ccgcgcggac
     1261 tcggcaacgt gctgtccgtg ttggacgcca cccacaatgc gccggcgcgt actgccagcg
     1321 ctgcttcggc gaggtcggcg taccgcagct cgggtgagta cccctgcacg acggcgtccg
     1381 gccgatcatc tgatgaagtg acgacccgca gtccaaccag ttcgacggcg gtgcgcaatg
     1441 ccgcacttcc cgtcaccagc accgacgcac cgacaccgag gcgttcgaca agcaccgtgg
     1501 cggctgcctg cgacgacgtg acgacgtcgc cggcttccgc gggcactccc agcgcaacca
     1561 gatgcgcagc tacggcagcg ggctcgcgcg acgcattgtt ggtcacgaat gccagccgca
     1621 taccggctgc gcgcgccttc gccagggcgg gcgcagcact agggattggg tcgggaccga
     1681 ggtagacgac accgtcgagg tcgaggaggg ccacgtcgta tgtagtcgca agtggggtgc
     1741 tgattcccaa caggctgcgg cccgcggcac gggtcacgtg gctccttcga gtcgggcgcg
     1801 gaccatccgc agctcccgac cgtagtgggc tagatcaggt cgcatggcgg cagccagcgc
     1861 gagatgctcg acagcggtgc gcaactcacc gatccgcgcc gcgctaagtc cgagtgcgaa
     1921 ctggccgtag tcgtcggtgg gatcggcctc gatgatccgg ctgaacgtcg cctttgcctt
     1981 cgcgtagtgg cgtgcttcga actgggcacg gccgagtgcc tcgagcacac tgcgcgagtc
     2041 cggttcagcc gtggcagctc gggccaacaa ctgctcggca gcccccgcgc tgcccgcctc
     2101 gagcagcgca agcccgcgtg tgtaccagtc gtaaggcccc tcgccgtcca ccgatgggct
     2161 tggttcgtcc acgcggcctc ctcactccac gaccactagc atgagcgcgc cgggcgccga
     2221 cgtcgtactc gcgtcgccta ccacgggcac tcacagggag caccatgacc gcgtcgccga
     2281 cagggacatt gcagctgctc ccctttcgtg cggtccgtta caacacgagc gtctcagatc
     2341 tcgccgcggt gctcgcaccg ccctacgacg tcatcgacga tgcagggcag gccgcactcg
     2401 aagctaccga tccgcacaac gtggtgcgcc tgatcttgcc gcgtgatccc gccatccccg
     2461 gcctcgatcg ctacgcggcg gccgcgcaga ccttcggaca atggttggac gacgaaaccc
     2521 tgctggttga cgacgctccc gccctctacg tgtaccagca aacccgggca ggtgccgtgc
     2581 tgcagcgcgg gttgctcggt gcggtcgtca tgcagcccat cgacgggggc gtcatcttgc
     2641 cgcacgagaa cgtcagatcc ggcccggttg aggatcgcct agcgctgatg cgagccatgc
     2701 gcgcaaatcc cgaacctatc tttctgctct acgacggcgg cggggcggcg acggcgagta
     2761 gtgcgtcggc cgtggcccgc gacccggttg cctcgacccg cacgtcggac gggatcaagc
     2821 acgagctctg gtcgatcacc gacgtcgcgg agttggccgg gatcgcagcc gacctcgcag
     2881 gccggcacgc gatgatcgcg gatggacacc atcgctacac gacgtacggg tacctgcgcg
     2941 acgagctgca tgcggcgggc gaaggcgctg gcccgtggga cgcgggactc accctccttg
     3001 tcgatgccag tgtggcggcc ccggatatcc gtgcgattca tcgggtggtg ccgcagctac
     3061 cgctcgatga ggcgctcatt gcggccgcag acgccttccg gattgtccgt ctcaacgcgg
     3121 acgtcgacgg cgtggaccgt gccctcgcac tcctgagtga gcacgacagc cctgcatttc
     3181 tgctcggcga cggccgctcg ttcgcacttc tcctcgatcc cgatccagac cgcctcgcgg
     3241 cagcaaaacc gagtgatcgg gcgccggcct ggtgggctct ggacgcgagt atcgcgagcg
     3301 tcttcgtcat ggagtcgctc tttggagtct cggatgcggc ggggcaggtc gaggccgagc
     3361 acgatcccgc ggcagcactg cgactggcgc aacgcgacaa cggtattgcg ctgtttctca
     3421 agcccgctcc cctggccgga atcgtcgccg tcgccgaggc cggcgacgcc atgccccgca
     3481 agtcgacgtt gttcttgcct aagccccgat ccggcatggc gatgcgtgca ttccgattcg
     3541 agggatgacg gatcagctgg ttgcgccgat ccttctgatc tcgagaacct gcacctcgca
     3601 ccgactcgcg gcgccttgcg cgccgcgcca gaatcggcgc cgcagttcgc cgcggacctc
     3661 gatcacctca ccgccgatcc atcgagccgc aacccgtcgc acgccggcct tgaaggcaac
     3721 gcaatcgatc gtgtcgacct tgggcaactg ccgaccgctg ggcgacagac caccgttgtc
     3781 acggccgacg accagccgcc agctgaccat ctcatcgccg ctcggaagaa cccgaacggt
     3841 ggtcgcccca gacacccgac ctcctagaac gacctcgttg tgtccctcga ctacggcgac
     3901 gcgacccgac ttggctcaat ttagtgcaca cctt
//
LOCUS       DATCBJ010000031         2057 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_69793, whole genome shotgun sequence.
ACCESSION   DATCBJ010000031 DATCBJ010000000
VERSION     DATCBJ010000031.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2057)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2057)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2057
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_69793"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            30..>2057
                     /locus_tag="VII50_00630"
     CDS             30..>2057
                     /locus_tag="VII50_00630"
                     /inference="COORDINATES: protein motif:HMM:NF024777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LamG-like jellyroll fold domain-containing
                     protein"
                     /protein_id="HEY5049375.1"
                     /translation="MSTFNPTASALVSAMVLAPDGSRVVVAGKFDHLDSDARTGLGAV
                     DAITGADDPWDASNPIADDGATSDLTSLTADATQVYGGGYFFTHGGNYEGRFAVNADT
                     GAIIWMNSCHGDTYATFPIGQVLYSASHEHECSDIGAFSQTTPGPIQAVHHFGEAETT
                     YATGTLHPAIFTLGGGPTYYNFGGQPSGTQLDWYPTFTEGTYTGQSQAAWSVSGNTHY
                     VSYGGEFPTVNGTAQQGLVRFAVSSIAPNKLGPTGLVAPTVVSQSSGTARIGWEAASD
                     QDNTALTYAVTRDSSTTPIYTTTVDSTFYNLPALSYVDKNLTPGSTHTYKVRVTDPFG
                     NTVLSAATSVTIGSATPDAYTASVLASGPVAFWPLSEPGGSALAYDHAGGADLTMGAH
                     GTFGVAGPHAGSTAVSFQGDPTIPSSFIPPSTIVPPVYSASTSGSVATAPPLATYSVE
                     AWVKTTSTTGGMIAGDGLWQTNDSQVEDRVLYFDTSGDLHFGAFNNGPTDAGLDRAIA
                     SPTAYNDGSWHYVVGTLGAGGAALYVDGVKVASDPTMVDTSVYFGHWRVGGDVIDGLP
                     LAGVPGFPAAPANGYLAGSIADVAVYPTALSASTVLSHFNGGAAGSPVAAFSSNCTAL
                     SCAFDASGSTDTSGTITSYSWDFGDGSAPVTGENPTHVYATAQTYNGTLTVT"
BASE COUNT          365 a          737 c          625 g          330 t
ORIGIN      
        1 tgtcgtggaa cccgtcgacc agcgcaaccg tgagtacgtt taacccgacc gccagcgcac
       61 tcgtgagtgc catggtgctc gcgcccgatg gctcgcgggt cgtcgtggcg ggcaagttcg
      121 accacttgga cagcgacgcc cgcaccggtc ttggtgcagt cgacgcgatc accggcgccg
      181 acgacccgtg ggatgccagc aacccgatcg ccgacgacgg tgcgacgtcg gacctgacca
      241 gcctcaccgc cgacgcgacc caggtctacg gcggcggcta cttcttcacc cacggcggca
      301 actacgaggg ccggttcgcg gtgaacgccg acaccggcgc gatcatctgg atgaacagtt
      361 gccacggcga cacctacgcc accttcccga taggtcaggt gctctacagc gccagccacg
      421 agcacgagtg ctccgacatc ggcgcgttct cccagaccac accgggcccg atccaagcgg
      481 tgcaccactt cggggaggcc gagacgacgt atgcgaccgg caccctgcac ccggccatct
      541 tcaccctcgg tggcggaccg acctactaca actttggtgg ccagccctcg ggcacccagc
      601 tcgactggta cccgacgttc actgagggca cctacaccgg ccaaagccaa gcagcgtggt
      661 cggtgtccgg caacacccat tacgtctctt acggcgggga gttcccgact gtcaacggca
      721 ccgcgcagca gggcctcgtg cggttcgcgg tctcctcgat cgcgccgaac aagctagggc
      781 caacgggttt ggtagccccg accgtcgtct cgcagtcgtc tggtaccgca cgcatcgggt
      841 gggaagctgc atcagaccag gacaacaccg cgctcaccta tgccgtgacg cgtgacagca
      901 gcacaacgcc gatctacacg accacggtcg actcgacgtt ctacaacctt cctgcactct
      961 cctacgtcga caagaacctg acaccaggct cgacccacac ctacaaagtg cgggtcaccg
     1021 atccgttcgg caacaccgtt ctgtcagcgg caaccagcgt gacgatcggc tcggcgacac
     1081 ccgacgccta taccgcaagt gtgctcgcaa gtggccccgt cgcgttctgg cctctgtcag
     1141 agccgggtgg cagcgcactc gcctacgacc acgccggtgg cgcggacctg acgatgggtg
     1201 cccacggcac cttcggagtc gccggcccgc acgcgggcag caccgcggtg tcgttccagg
     1261 gcgatccgac gattccaagc tcattcattc cgccgtccac gatcgttccg ccggtgtact
     1321 cggcaagcac gtccgggtcg gtcgctaccg ctccgccgct ggctacgtac agcgtcgaag
     1381 cgtgggtgaa gaccacgtca accacgggcg gcatgatcgc cggtgacggt ttgtggcaga
     1441 cgaacgacag ccaggtcgag gatcgcgtgc tgtacttcga caccagcggc gacctgcact
     1501 tcggcgcctt caacaacggt ccgactgatg ctggtctcga ccgggcaatc gccagcccga
     1561 ccgcgtacaa cgacggcagc tggcactacg tcgtcggtac cctcggcgcc ggtggagctg
     1621 cgctctacgt ggacggcgta aaggtcgcca gcgacccgac gatggttgac acgtcggtct
     1681 acttcggcca ctggcgggtc ggcggcgacg tgatcgacgg cctgccgctt gccggcgtgc
     1741 ccggttttcc ggctgcgccc gcgaacggct acctggccgg atcgatcgcc gatgtcgcgg
     1801 tatacccgac cgcactgtcg gcgtccactg tcctctcgca cttcaacggc ggtgcggcag
     1861 gctcaccggt agctgcgttc agctcgaact gcaccgcgct gtcgtgcgcg ttcgatgcgt
     1921 cgggctcaac cgacactagt ggcaccatca ccagctacag ctgggacttc ggtgacggca
     1981 gcgcgccagt cacgggtgag aacccgaccc acgtctacgc caccgcccag acgtacaacg
     2041 ggacgttgac cgtcacc
//
LOCUS       DATCBJ010000032         2306 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_400472, whole genome shotgun sequence.
ACCESSION   DATCBJ010000032 DATCBJ010000000
VERSION     DATCBJ010000032.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2306)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2306)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2306
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_400472"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..482)
                     /locus_tag="VII50_00635"
     CDS             complement(<1..482)
                     /locus_tag="VII50_00635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5049376.1"
                     /translation="MRDMPRSAQAYVYVVVCAGLAVIIAASASGRVNWAAVAVLGALY
                     WLADSREIITRTGVLGVSASSPVALASLLLLGSWGGALVCAVSALAYARHAAWFKHVF
                     NGAQAALSAALAGCIYHFVPGAAKDGFVHASFPAVLPAVAVAGLSYFVLNNLFVAVVV
                     R"
     gene            1258..1923
                     /locus_tag="VII50_00640"
     CDS             1258..1923
                     /locus_tag="VII50_00640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5049377.1"
                     /translation="MGDPTGPAASAATAASATSAASLDRYTALVAALLGVRTDYASAR
                     FDAEVAAALAANRVDVETARTLRWWQRASVREVEGYAGSVLPVVLATRSDADTVAAMD
                     SDESAASWQQAQTITPSPTPSTVSASLSATLSATLSANAATTVVRPEVAEPPRPSRVI
                     TIPDPSTRRRSASEIQQAVRAALRATAPPLDAGPPFEAPSPQDASSQRRDRGDHAHTA
                     PTA"
     gene            1898..>2306
                     /locus_tag="VII50_00645"
     CDS             1898..>2306
                     /locus_tag="VII50_00645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5049378.1"
                     /translation="MPTLRLLPELEEALRQVPGIRAASVVTGPDAVPTEIHIVASRSK
                     GAKQVVRDVQSLAMAGYDIDIDHRIVSVVQFDDDIDGVQADDNLHTIIRMPDTAPAID
                     TLPRPVIAAITIRTAGSEADASVVVRTGDASYDG"
BASE COUNT          471 a          777 c          724 g          334 t
ORIGIN      
        1 cgaacaacca ccgccacgaa caagttgttg agcacgaagt acgacagccc ggcgaccgcg
       61 accgccggca ggacagccgg gaaactcgcg tgaacaaagc catccttggc cgcgccgggc
      121 acgaagtggt agatgcagcc ggcgagggca gcgctcagcg ctgcctgcgc accgttgaaa
      181 acgtgcttga accaggccgc gtgccgggcg taggcgagag cgctcacagc gcagacgagg
      241 gcgccgcccc atgatccaag aaggagaagc gacgcgagag cgacagggga gctcgcagaa
      301 acaccgagca ccccagtcct ggtgatgatc tcgcgcgagt cggccagcca gtacaaggca
      361 cccagaaccg cgaccgccgc ccagttcact cggccagagg cgctcgcggc gatgatcacc
      421 gcaagtccgg cgcacacgac cacatagacg tatgcctgcg cgctgcgcgg catgtctcgc
      481 atggtctctc ctgccaggga accagcggtt cgaggcgggc tgagaaacgt tgcccggccg
      541 agaagccgac gtgtgggtgc ggggggaagc gcaggaaccg tacggaagct gggaagcaga
      601 aagctgccgg gagacgaaga acgaaacggg acttaggtca accttgctct acgtgcactt
      661 gctctaactc cacttggtgc tagcgccgag gaagaacttt tcgacaacgg cggtgatctt
      721 ctgcatggat ctcagctcct acgaatcacg tcataggcgc cgctaaggcc tacgcgagat
      781 acgctccgct aaaggagtag aaactgcgta aatacgccca agtcccgttc gctctgcagt
      841 tgttttgcga cccgggaaat tgggggctcc ccgggggcac aaacggcgat ctttgcaccg
      901 gactcgaacc gaaattcgag ggatgaaaga gcgtccgcat aaggagtgaa tcggtgaaga
      961 tcagggcaag tcaagaccct gcagacactg cagccacacg ggtttcgggg cactccttgc
     1021 tggccagtca taaaggacta gaccgcgtac gacaaggatg accgctcaat acgatacgga
     1081 tgacggtgaa ataccgacca tcaccctgtt catccgttta atgccactct ggtagggccg
     1141 cagccaccag caccactgtc acaacactgc aggacctaag ggactcccgc gaaaccatcg
     1201 cggcacccta cactcgggtc cggtgtgtga cggatgtgac gattggggct acttgccatg
     1261 ggcgacccga ccggtcccgc ggccagtgcg gcgactgccg cgagcgcgac cagcgccgcg
     1321 tcgctggatc gctatacggc actggttgcg gccctgctcg gcgtccggac cgactacgca
     1381 agtgcccgct tcgacgccga ggtagcggct gcgctcgccg cgaacagggt agacgtcgag
     1441 accgcacgca cgttgcgctg gtggcaacgc gcgtccgtgc gggaggtcga ggggtacgcg
     1501 ggcagcgtcc tgcccgtggt actggcaacc cgctccgacg ccgacaccgt ggctgcgatg
     1561 gacagtgacg agtccgcggc gtcgtggcag caggcccaga ccatcacgcc cagccccacg
     1621 ccgtccaccg tgtcggccag cctctcggcc acactgtcgg ccacactgtc ggccaacgct
     1681 gcgaccaccg tcgtccgccc cgaggtcgcg gaacccccgc ggccgtcgcg tgtcatcacc
     1741 attcccgatc cctcgaccag acggcgatcg gccagtgaga tccagcaggc tgtccgcgcg
     1801 gcgttgcgag ccactgcccc gccgttagat gcaggcccgc ccttcgaggc gccctcaccc
     1861 caggacgctt cttcgcaacg aagggatcgt ggcgaccatg cccacactgc gcctactgcc
     1921 tgaactcgag gaagccctac gccaggtacc cggcatccgg gcggccagtg ttgtgacggg
     1981 acccgacgcc gtccccacgg aaattcacat cgtcgccagc cgcagcaagg gcgccaagca
     2041 ggttgtccgc gacgtccagt cgctcgcgat ggcgggctac gacatcgaca tcgaccaccg
     2101 catcgtgagt gtcgtgcagt tcgacgatga catcgacggc gttcaggccg acgacaacct
     2161 gcacacgatc atccggatgc cggacacagc gccagcgatc gacaccctgc cgcgcccggt
     2221 catcgccgcg atcaccatcc ggaccgcggg ctcggaggcc gacgcatcgg tcgtcgtccg
     2281 gaccggcgat gcgtcttacg acggcc
//
LOCUS       DATCBJ010000033         2298 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_31530, whole genome shotgun sequence.
ACCESSION   DATCBJ010000033 DATCBJ010000000
VERSION     DATCBJ010000033.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2298)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2298)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2298
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_31530"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..615
                     /locus_tag="VII50_00650"
     CDS             <1..615
                     /locus_tag="VII50_00650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018538739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha
                     C-terminal domain-containing protein"
                     /protein_id="HEY5049379.1"
                     /translation="TVERGRGYVQANGNKQPSHEIGRIPIDSIYSPVLKVTFKVEATR
                     VEQRTDFDKLIVDVETKPCMRPRDAVASAGKTLVELFGLARELNIDAEGIDIGPSPTD
                     AALAADLALPIEDLNLTVRSYNCLKREGIHTVGELVSRSEADLLDIRNFGAKSIDEVK
                     AKLIEMGLNLKDAPPGFDPSSVVYAGDDDDADFDDQGYAETEQL"
     gene            706..1056
                     /gene="rplQ"
                     /locus_tag="VII50_00655"
                     /pseudo
     CDS             706..1056
                     /gene="rplQ"
                     /locus_tag="VII50_00655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016293478.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
     gene            1443..>2298
                     /gene="truA"
                     /locus_tag="VII50_00660"
     CDS             1443..>2298
                     /gene="truA"
                     /locus_tag="VII50_00660"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HEY5049380.1"
                     /translation="MDEPGHPDQGRTGLVRLRVDFGYDGTAFFGWARQPGLRTVQGVL
                     EPAFATVLRLPTPPLLTIAGRTDAGVHARGQVAHVDVPANAESLLAMLAGDGGPDAVA
                     RRLNGVLGLDVRVRSVTVAPSGFHARFAALSRRYSYRVADHWFFPPRRDDTLAHPHPL
                     DLDAMNVAASLLLGEHDFAAFCRRREGATTVRTLLVLQWVRDDVGVAIARVEADAFCH
                     SMVRSLIGTLLEVGDGKRDVEWPAAVLAGRDRSAAARVAAAHALTLEEVRYPPDADLF
                     ARTQVTRHR"
BASE COUNT          404 a          768 c          787 g          339 t
ORIGIN      
        1 actgtcgagc gcggtcgtgg ttacgtgcag gcgaacggga acaagcagcc aagccacgag
       61 atcggccgca tcccgatcga ctcgatctac tcccccgtct tgaaggtgac cttcaaggtt
      121 gaggcgaccc gagtcgagca gcgcaccgac ttcgacaagc tcatcgtgga cgtcgaaacc
      181 aagccgtgca tgcgcccgcg cgacgcggtt gccagtgcgg gtaagacgct cgtcgagttg
      241 ttcggtcttg cacgcgagct gaacatcgac gccgaaggca tcgacatcgg tccgtcaccg
      301 accgacgccg cacttgccgc cgacctggcg ctgccgatcg aagacctcaa cctcacggtg
      361 cggtcgtaca actgccttaa gcgtgaaggc atccacacgg ttggcgagct ggtctcgcgt
      421 agcgaggccg acctgctcga catccgcaac ttcggtgcga agtcgatcga cgaggtcaag
      481 gccaagctca tcgagatggg cttgaacctg aaggacgcac ctcccggatt cgacccgagc
      541 agcgttgtgt acgccgggga cgatgacgat gccgacttcg atgaccaggg gtacgcggag
      601 accgaacaac tctgatctga actcgaccgg ttaccgggca ctgactgtta ccggagaact
      661 gaagttacct gagaactgaa gtattgacct taggaaggcc aggacatgcc gacgcccacg
      721 aagggacccc gcctcggcgg cggctcgtcg cacgagcgga tcattctcgc gaacctcgcg
      781 accgcgttgt tcgagcacgg ccacatcacg acgacgctgg ccaaggccaa gcggctgcgt
      841 ccggtggccg agcgcctggt caccaaggcc aagcggggcg acctgcactc gcgtcgccag
      901 gtgatgacgg tcgtgcgcga caagacggtc gtgcacggtc tgttcaccca gatcggcccg
      961 cgctacctca accggcccgg cggctacacc cgcatcgtga agctcgggcc gcgcaagggc
     1021 gacaacgcgc cgatggcggt catcgagctg gtcgagccgc tggcggagca agtggtcgcc
     1081 gaggcaaccg gggcaacccg ccggggcgcc aaggagcgca ccgtacggac gcccgctgcc
     1141 gcaaccacga gcaaggccgc cgagctcgag gctctcgacg cgaaggcgga agcggcggcc
     1201 gaggctgccg aggacgccga ggctgaaggt gacgtcgaag gtgccgtagc cgcggccgcg
     1261 gaagcggacg aggcggcagc gcaggccgaa gagctcgctg gggagatcgg cgacgatgcg
     1321 tcggccgagg tcgccggtga cgccctggcg ttcgaagaga tcgcggacga ggctgccgac
     1381 gcagccaaga aagccgcggg cacctccgag gacgacgcct gagcaacagc agcacgcaga
     1441 acatcgacga gcccggccat cccgatcagg ggaggaccgg gctcgttcgg ctacgggtcg
     1501 acttcggcta tgacggcacc gcctttttcg gctgggcccg ccagccgggt ctgcggacag
     1561 tgcagggcgt cctcgagccg gcgttcgcca ccgtgttgcg cctgccaacg cctccacttc
     1621 tcaccatcgc gggtcgaaca gacgccgggg tgcacgcgcg tggccaggtc gcgcatgtcg
     1681 acgtgcccgc taacgcggaa tccctgctgg cgatgctcgc cggcgatggc gggccggacg
     1741 ctgtcgcgcg ccggctcaac ggcgtcctgg ggctcgacgt ccgggtgcga tcggtcacgg
     1801 tagcgccgag cgggttccac gcacggttcg cggcattgag ccggcgttac tcctaccggg
     1861 tcgccgatca ctggttcttc ccgcctcgac gcgatgacac gctcgcgcat ccgcacccac
     1921 tcgatcttga cgcgatgaac gtggcggcct cgctgcttct cggcgagcac gacttcgccg
     1981 ccttctgccg ccggcgcgag ggagcaacga cggtacgaac gctgcttgtg ctccagtggg
     2041 ttcgcgacga cgtcggggtc gctatcgctc gcgtcgaagc cgacgcgttc tgccactcga
     2101 tggtgcgctc gctcatcggg acgttgctcg aagtcggaga cggcaagcgg gacgtcgagt
     2161 ggccggccgc cgttttggcc ggcagggacc gatcagcggc cgcgcgcgtt gcggccgcgc
     2221 atgcgctgac gctcgaggag gtccgctacc cgccggacgc cgacttgttc gcgcgcactc
     2281 aggtgacgcg gcatcgtc
//
LOCUS       DATCBJ010000034         5964 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_288923, whole genome shotgun sequence.
ACCESSION   DATCBJ010000034 DATCBJ010000000
VERSION     DATCBJ010000034.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 5964)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5964)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5964
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_288923"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            66..1079
                     /locus_tag="VII50_00665"
     CDS             66..1079
                     /locus_tag="VII50_00665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HEY5049381.1"
                     /translation="MTSGPMLAALPDGTSRALAAAVAARLIGNVERVVHGRRAAVELV
                     TAGLLVGGHVLVQDVPGSGKTTLARAFARSVGGSFRRIQGTADLLPGDITGSAMWDPD
                     QHSFVFVPGPVFANVLLVDELNRATPRAQSALLEALDEGAVTVDGVRHALPEPFFVVA
                     TQNPTDQYGTYPLPEGQLDRFSVALTLGENDLATERQVVREQLLRPTVDDLQPVVSPH
                     DLRWVQDEVRRTHVADGVLDFALNVIRATRRHPAVRIGASSRAALSLVRCAQARTLVY
                     DREFVVPDDVKVLAPYVLGHRLVLRRGVVAQGSRGAGFDALGAGSAVVAEVLASVPVP
                     VRG"
     gene            1072..2205
                     /locus_tag="VII50_00670"
     CDS             1072..2205
                     /locus_tag="VII50_00670"
                     /inference="COORDINATES: protein motif:HMM:NF013998.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HEY5049382.1"
                     /translation="MAESVVPAAGARRVRITSNGWAAVAGAGLMFAMAIQTQSIWMQV
                     VGSALLGLLGISLLSVMRRRNGVTVSMQAPANVMVGVPFDIQVVVSNTGQHPTSPLRI
                     RYDVNIEPPPVAPAVVYVDPVASGDKAVLSVTRVPLRRGAAKSSRLVVDVIGPFGFFT
                     SSYRVDGTPGLFAAPLASRPIDVARALSTDAGGSGPMGAGLDVRGVREWRPGDAVRHV
                     HWRSTARTGRLAVLEYGEPTVDMFGVLIAGTRATDPRFEAELALAASTTWRGLDDGVM
                     VVIAFADDAIANAQYLMSVAAEPELLRLTPTQYHMIFASLRAAVPDPGTVDGLLAYVG
                     VGGVVLLATGDGLADEFLSYVESAASVAGVRLVRVADYLDAVR"
     gene            2202..4322
                     /locus_tag="VII50_00675"
     CDS             2202..4322
                     /locus_tag="VII50_00675"
                     /inference="COORDINATES: protein motif:HMM:NF023418.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminaseTgpA domain-containing protein"
                     /protein_id="HEY5049383.1"
                     /translation="MTRPARSPAVVNAHATRLALLIGGAAAQFAIMPLLSGVSAAFAL
                     GSYLAVGLATLRLPLPKVAPGGRSGGFRLTMAPRQATALAVMMVGAVMVLARVNTFDP
                     NSKAAALTTLLLVVQVAHCLALQTRREAALGCAIIIVMLSVGAAFAGDVTLLLPVMVA
                     LPAVAVTASLLHRGALIDGADVTSAGGVGSIVRACVTPVVLATVLGLVVFLVLPDTGH
                     LRTHARLASTAGNSDPSSGPASGGSSGRAASDPGAGTIDLGLRGSLSNAAVFEAPANS
                     PAYWQGAIFSNFDGQSWTAHGPFTEWTSGPGATQLAPVDPTSQADDVVSTYTVRVLMT
                     TPLDVVLAPGHPTSYLGVGRVIDDTDGTPHVTYDSATFGAATYIVSSTLPKDAANPDS
                     VASTGSDLTNPQWLEIPADLPGRVSALAASLVAGSTSRLNAVNSVEDYLRTTEKYNLN
                     SPLPKPGDDAVDDFLFVSHQGFCEQFATAAVVMLRSQGIPARFVTGYAVGDSTIDPGE
                     RVFRGSDAHAWVQVFYPGVGWVNSDPTASAVLQTSVPSLRQRIGASMTRLWHRVPGGR
                     WGAVVALALAVVIGAALSTIGRRWLRRRRRYIGIDRGRHGDGPILAAYLRLDIALHGV
                     ERARAPSESLGELAGRLGGFVATPGEVAAAISYLERETYSVDPPSGAESAAAIAVFDR
                     LRLGAGSQLVAVAVGTGTGTNSAR"
     gene            complement(4348..5007)
                     /gene="nucS"
                     /locus_tag="VII50_00680"
     CDS             complement(4348..5007)
                     /gene="nucS"
                     /locus_tag="VII50_00680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018550058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease NucS"
                     /protein_id="HEY5049384.1"
                     /translation="MRLVIARCSVDYVGRLTAHLPSAQRLILVKADGSVSIHADDRAY
                     KPLNWMSPPCTLQEAPGVWTVTNKAGEQLVLTIEELLHDSSHELGVDPGLVKDGVEAH
                     LQVLLAAHPGALGEGMTLVRREYPTPIGPVDLLCRDATGVSVAVEIKRRGEIDGVEQL
                     TRYLELMNRDALLAPVRGIFAAQEIKPQARVLALDRGIECITIDYDALRGADDPTARL
                     F"
     gene            complement(5077..5550)
                     /locus_tag="VII50_00685"
     CDS             complement(5077..5550)
                     /locus_tag="VII50_00685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HEY5049385.1"
                     /translation="MPSSRGVAERKADYAAARKAESGFTLIELLVVMIIIGILAAIAI
                     PLYLSQKQKAADTQAKSDVHSAAIAEESYFADFLTYVPATTSTTPPAVLAAQGFKMSS
                     KTVDVTVYTYPQGATTGGSTSLSQSGGYCVRVTSGSSKAFYYASFLGGITNNACG"
     gene            complement(5594..>5964)
                     /locus_tag="VII50_00690"
     CDS             complement(5594..>5964)
                     /locus_tag="VII50_00690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="molybdopterin dinucleotide binding
                     domain-containing protein"
                     /protein_id="HEY5049386.1"
                     /translation="RPGPGCLILQTIRSHDQYNTTIYGLNDRYRGIHHGRRVVFVSPD
                     DIATLGLADGDMVDLVSVWIDGTSRRAPAFRVVSYPTARGCAAAYFPETNVLVPLDST
                     ADTSNTPTSKSIIVRLEPAP"
BASE COUNT          889 a         1899 c         2037 g         1139 t
ORIGIN      
        1 gcccttcatg agtgccgata ggcggccgat gagacaaggg accagtcgat gagcccggag
       61 gacctatgac gagcggccct atgctggctg ccctgcctga cggcacctcc cgcgcgctcg
      121 cggcggcggt ggcggccagg ctgatcggca acgtcgagcg ggtcgtgcac ggccgccgcg
      181 ctgccgtgga gttggttact gccggccttc ttgttggtgg ccacgtcctc gtgcaggacg
      241 ttcctggcag cggcaagacg actctggcac gcgcgttcgc gcgttcggtc ggtggcagct
      301 tccggcggat ccagggcacc gctgacctgc tgcccggcga catcaccggc tcggcgatgt
      361 gggacccgga ccagcacagc ttcgtattcg tgcccggtcc tgtcttcgcc aacgtgctgc
      421 ttgttgacga actgaaccgt gcgactccgc gtgcgcagtc cgcgttgctc gaggcccttg
      481 acgaaggcgc agtcactgtc gatggcgtcc gccatgcatt gcctgaaccg ttcttcgtgg
      541 ttgcgaccca aaatcccacc gaccagtacg gcacataccc gttgcccgaa ggacagctcg
      601 accggttctc ggtcgccctc acgctgggcg agaacgatct cgcgaccgag cgccaagtag
      661 tccgtgagca gctgttgcgg ccgactgtcg acgatctgca gcccgtggtc tcgccccacg
      721 acctgcggtg ggtgcaggac gaagtgcgcc gaacgcacgt ggccgacggc gtcctggatt
      781 tcgctttgaa cgttatacgc gcaactcgcc gtcatccggc ggtacgcatc ggcgcgagct
      841 cgagagccgc gttgtcactt gtgcgatgtg cgcaggcgcg aacgctcgtc tacgaccgtg
      901 agttcgtcgt gccggacgac gtcaaggtcc tcgcgccata tgtactcggg caccgccttg
      961 tcctgcggcg cggggtggtc gcccagggat cgcgaggtgc aggcttcgac gccctcggcg
     1021 ccgggagcgc ggtggttgcc gaggtgctgg cctcggtgcc ggtcccggtc cgtggctgag
     1081 tctgtcgttc cagccgccgg cgcacggcgg gtgcggatca cctccaacgg gtgggctgcc
     1141 gtggccggcg cgggcctgat gttcgcgatg gccatccaga cgcagagcat ctggatgcag
     1201 gtcgttggca gtgcgctact gggtcttctc ggcatctcgt tgctttccgt catgcgccga
     1261 agaaatggtg tgacggtcag catgcaggcg ccggccaacg tgatggtcgg tgtccccttc
     1321 gacatccaag tagtcgtgag caataccggc cagcatccca cttcgccgtt gcgtatcagg
     1381 tatgacgtga acatcgagcc gccgccggtg gcgcccgcgg ttgtgtacgt cgatcccgtc
     1441 gcgtcgggcg acaaagctgt cctgtccgtg actcgggttc cgcttcggcg cggagcggcg
     1501 aaatcgagtc gcctcgtggt cgacgtgatt ggaccgttcg gctttttcac ctcctcctac
     1561 cgggttgacg gcacgcctgg gctgtttgca gctccgcttg cgtcccggcc gatcgatgtc
     1621 gcgcgggcgt tgagcacaga cgctggtggt tccggcccga tgggcgcagg actggacgtg
     1681 cgtggtgtgc gtgagtggcg gccgggggac gcggtgcgtc acgtgcactg gcgttcgacg
     1741 gcgcgcaccg gccgcctggc ggtcctcgag tacggcgaac ccactgtcga catgttcggc
     1801 gtgctgattg ccgggacgag ggccaccgac ccgcgcttcg aagccgagct cgctctcgca
     1861 gcgtcgacca cgtggcgagg cttggacgac ggtgtcatgg tggtcattgc attcgccgat
     1921 gatgcgatcg cgaacgctca gtacctcatg agcgtagccg cggaacccga actgctgcgg
     1981 cttacgccga cgcagtatca catgatcttc gccagcctgc gcgcggcggt tcccgacccc
     2041 ggcaccgtcg acggattgct cgcatacgtt ggagtcggcg gcgtcgttct gctggcgacc
     2101 ggcgacggac tcgccgacga attcttgtcc tacgtcgagt cggcggcgag cgttgccggc
     2161 gtgcgcctcg ttcgcgtcgc cgactatctg gacgctgtgc gatgacccga ccagcgcgga
     2221 gccccgccgt cgtcaatgcc cacgccaccc gtcttgcctt gctcatcggc ggtgctgctg
     2281 cacagttcgc gatcatgccg ttgctcagcg gagtctcggc ggctttcgca ctcggctcct
     2341 accttgcggt tgggttggca accttgcggc ttccgctgcc caaggtggca ccaggcggtc
     2401 ggtcgggagg cttccgactg acgatggcgc cgcggcaggc gaccgcactc gccgtcatga
     2461 tggtgggcgc ggtcatggtt ctcgcgcggg tgaatacgtt cgacccgaat tcgaaagcag
     2521 cggcgttgac gacgctgctc ctcgtcgtcc aggttgcgca ttgtcttgcc ctgcagaccc
     2581 gccgagaagc cgcgctcggc tgcgccatca taatcgtgat gctctcggtg ggcgcagcgt
     2641 tcgcgggcga cgtgaccttg ctcttgcctg tgatggtggc gttgccggcg gtggcggtga
     2701 ccgcgtcgtt gctgcatcgc ggcgccctca tcgacggcgc ggatgtgaca tcagccggcg
     2761 gtgttggctc gatcgttcgt gcctgcgtga cgcccgtggt gctcgcgacg gttcttggac
     2821 tcgtcgtgtt cctggtgttg ccggacaccg ggcatctgcg cacccacgcg cgcctcgcca
     2881 gtactgcagg caactcggac cccagttcgg gtccggcgag cggcggatcg agtggccgcg
     2941 ctgcgagcga tcctggagca ggcacaatcg acctcgggtt gcgtggatca ctgagcaatg
     3001 cggccgtgtt cgaggcgcca gctaactcgc ccgcgtactg gcaaggtgcg atcttcagca
     3061 actttgacgg gcagtcctgg accgcgcacg gaccgttcac ggagtggact tccggtcctg
     3121 gcgcgacgca gctggcaccg gtcgatccga cgtcgcaagc cgacgatgtg gtgagcacgt
     3181 acaccgttcg ggtgttgatg acgacaccgc tcgatgttgt cctggcaccg ggccacccga
     3241 ccagctacct gggcgtcggc cgcgtgatcg acgacactga cggaacaccg cacgtcacgt
     3301 acgattcggc gaccttcggt gcagcgactt acatcgtttc ctcaacgctg cctaaggacg
     3361 cggcgaaccc cgactcggtc gcgtcaactg ggtccgatct cacgaatccg caatggctgg
     3421 agattccggc cgatctaccc ggccgcgtga gtgcgctcgc ggcgtcgctc gtcgccggct
     3481 cgacgagccg actcaacgcc gtgaactccg tcgaggacta cctccgaacg accgagaagt
     3541 acaacctgaa ctcgccgttg ccgaagcccg gcgacgatgc ggtcgatgat ttccttttcg
     3601 tgtcacacca aggcttctgt gaacagttcg cgacggccgc cgtggtcatg ctgcgcagtc
     3661 aggggattcc ggcgcgtttc gtaaccggct atgccgtcgg cgattcgacg atcgatccag
     3721 gcgaacgagt gtttcgtggg agcgacgcgc acgcgtgggt acaggtgttc tatccaggcg
     3781 tgggatgggt caacagtgat ccgactgcta gcgccgtctt gcagacctcc gtgccgtcac
     3841 tacggcagcg catcggtgca tcgatgaccc gactgtggca tcgtgttccc ggtggtcgat
     3901 ggggtgccgt tgtcgccctc gcgcttgccg tcgtcatcgg cgcggccctc agcacgatcg
     3961 gacgccgatg gctgcgccgc cggcgccggt atatcggcat cgataggggt cgtcacggag
     4021 atggtccgat cctggctgcc taccttcggc tcgacatcgc gttgcacgga gtggagcgcg
     4081 ctcgtgcgcc gagcgagagc ctgggcgagc tggccggtcg gctcggcggg ttcgtggcca
     4141 cccccggcga ggttgcggcc gcgatcagtt acctcgagcg cgagacgtac agtgtcgacc
     4201 caccatcggg cgcagagtct gccgccgcaa tcgcggtgtt cgatcggctt cgccttggtg
     4261 caggttccca gttggtcgcc gtcgccgtgg ggaccggaac cggaacgaac agcgcccgct
     4321 gaccgccgtc ccgctcgtca gcggggctca gaacaggcgg gcagtcggat cgtccgcgcc
     4381 gcgcaacgcg tcgtagtcga tggtgatgca ctcgatgccg cggtcgaggg cgagcacgcg
     4441 tgcctgtggc ttgatctctt gcgccgcgaa gatgccacgc accggtgcga ggagtgcgtc
     4501 gcggttcatc agctcgaggt agcgagtgag ctgctcgacg ccgtcgattt cgccgcggcg
     4561 cttgatctcg accgccacgc tcacaccggt agcgtcgcga cacagcaggt cgaccgggcc
     4621 gatcggtgtc ggatattcgc gacgcacgag ggtcatgccc tcgccgagag cgccggggtg
     4681 tgccgcgagc agcacctgta ggtgtgcctc cacgccgtcc ttcaccaggc cgggatcgac
     4741 cccgagctca tggctggagt cgtgcagcag ctcttcgatg gtgagcacga gttgttcgcc
     4801 ggccttgttc gtcacggtcc agacgccggg agcctcctgc agcgtgcagg gcgggctcat
     4861 ccagttcagt ggcttgtacg cgcgatcgtc ggcgtggatc gagactgagc cgtccgcctt
     4921 gacgaggatc aaccgttgcg cgctcggcaa atgggctgtc aaccggccga cgtagtcgac
     4981 gctgcatcgc gcaataacca gccgcacccc gacacgttag cgggtcacgg tagtggcaag
     5041 aacaccgatc ggacgacgtg ggcccgacga atcgggttac ccgcaggcat tgttggtgat
     5101 cccgccaagg aagctcgcgt agtagaaggc cttgctcgac cccgaggtca ctcttacgca
     5161 gtagccgccg gactgcgaga ggctcgtcga gccgcctgtc gtcgctcctt gtgggtacgt
     5221 gtagaccgtg acgtcgacgg tcttgctcga cattttgaag ccttgcgcgg ccagcaccgc
     5281 tggcggcgtc gtggaggtgg ttgccgggac gtaggtgagg aaatcggcga agtagctctc
     5341 ttcggcgatt gcggcgctat gtacgtcact cttcgcctga gtgtcagcag ccttttgctt
     5401 ctggctcagg tagagcggga tcgcgatcgc ggcgaggatg ccgatgatga tcatgacgac
     5461 aaggagctcg atcagggtga agcccgactc cgctttccgg gcagcggcgt agtccgcttt
     5521 gcgctcagcg actccgcggg aggacggcat gggtagttct tcggattccg gcgagccgca
     5581 ctttaggctt cctttaaggt gccggttcga gtcgcacgat gatcgatttg gacgtcggcg
     5641 tgttgctggt gtcagcggtg ctgtcgaggg gcaccaggac gttggtttcg gggaaatagg
     5701 cggccgcaca tcctcgcgcg gtcgggtagg agacaacccg gaacgccggc gcgcgccgcg
     5761 acgtgccatc gatccacacg ctcaccaggt cgaccatgtc gccgtctgcg agaccgagtg
     5821 tcgcgatgtc gtcagggctc acgaacacca cgcggcgacc gtgatggatg ccgcggtaac
     5881 ggtcgttgag gccatagatc gtggtgttgt actggtcgtg cgaacgaatc gtctgcagga
     5941 tgaggcagcc agggccaggc cgca
//
LOCUS       DATCBJ010000035         8938 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_546964, whole genome shotgun sequence.
ACCESSION   DATCBJ010000035 DATCBJ010000000
VERSION     DATCBJ010000035.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 8938)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8938)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8938
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_546964"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..609
                     /locus_tag="VII50_00695"
     CDS             <1..609
                     /locus_tag="VII50_00695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HEY5049387.1"
                     /translation="VADDGELLVRGEHVFDRYWADPDATAAARDADGFLHTGDLGAID
                     NDGFITITGRKKEILVTAGGKNVSPALLEDRLRGHPLIAQAMVVGDRRAYIAGLITID
                     EEALATWKTKVGKDPSLGLAELREDPDLLAEIQAAVDDANRVVSRAESIRRFRILDRD
                     FTEAGGQLTPTLKLRRNLVMTEFADEIEGLYSPTAPGTRMAP"
     gene            699..1418
                     /locus_tag="VII50_00700"
     CDS             699..1418
                     /locus_tag="VII50_00700"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY5049388.1"
                     /translation="MVARTPAYVETIGLRVAFGPVVAVHSLDLTVQPASAVALVGRNG
                     AGKSTTLRVLAGVLPATSGEVMVAGVDVTRDPATAKSHVGYCPDVGGLIPRATPWEHL
                     ALAAKLRGLRPDWKDRAHELLELFDLVGAADRVTAGFSHGMGRRLSVVLAVLHEPSVL
                     LLDEPFDGVDPLGVDATLEVISRARDNGCAVVVSTHLLELAAAVCDEVVVLRAGRIVA
                     AGPSAELRGEAGELRYRALLA"
     gene            1415..2917
                     /locus_tag="VII50_00705"
     CDS             1415..2917
                     /locus_tag="VII50_00705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5049389.1"
                     /translation="MTGAQLPALVRLRWQMVRAERVRVGLLVLAACVPGLLCAAVIAG
                     QLAPRSRSFDVTVLGPTAFLGFAVLSLVAPLAAGGGNELFPPEQLVAYPIRPATHFFE
                     SLLVAPLNLAWMSQFVLLFGMTSFVTGARPGLGLALVTTLVYVAMVTVAGQMVAWMLI
                     GARQSLLGRRAVWALTAAIVVTTLVVLHAHLGYRVLDNAPTKPVVSAVLAGGGHRYFA
                     WASGTTALGIILLGCLWCGPRICAWALRRPGDRTRFRESRVERRRPTPPSPLATLVRV
                     DRANVWRAAPLRRGLLVLAILPGVVAASAALRWDSLVLLPALVAAGAGLLYGVNMFCL
                     DAGGATWLASLPSPPRLAVTAKTIVLGETIGGSCLVAAIAGSVRAAGPRTAADLVAMA
                     CCLVSCTAVVLATCLRLSIRTPHRAELRGHRDTPAPPAAMAVYSVRLATVTTLIGLLF
                     SLAAHEQSFAACLVLASGLIALSGLSIAQSFRRWDDPSVRSRIVAAVSNG"
     gene            3107..3820
                     /gene="cpaB"
                     /locus_tag="VII50_00710"
     CDS             3107..3820
                     /gene="cpaB"
                     /locus_tag="VII50_00710"
                     /inference="COORDINATES: protein motif:HMM:TIGR03177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly protein CpaB"
                     /protein_id="HEY5049390.1"
                     /translation="MGRRTVLLVAAVLVAAIGAGLVLIYVHDVNQKVKAREAPEQILV
                     AKKVIPAGTTGAAASAEGDLEFLRVAREAVAPGALATIDPVANLVTLAPIYPGEQILS
                     LKFGKQGASSLLTIPTAGTVALSISLSGPAGVNGFIAPGSQVAVFLTAGGATKLILPR
                     VSVIAVGSRTLVPSSGQATGTNQQSDVVTFGVTAAQAQTLIYAQSAGSLYLTLLTANS
                     SVPSLPPTTATNVTSGAPS"
     gene            3845..5044
                     /locus_tag="VII50_00715"
     CDS             3845..5044
                     /locus_tag="VII50_00715"
                     /inference="COORDINATES: protein motif:HMM:NF013793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEY5049391.1"
                     /translation="MSVIVEPDPTRAETLRSALGPGVLVLKTLDDLRRHLEAHPGEDA
                     VVLGPSVDQDAAFHLSDSMRITRTSLGVVLVRRRIDTPLLVDALRNGIREVVEERNLA
                     GVNDAVRRVRDVAAAMRQRASHGEGEDGRRGVVVTVFSAKGGCGKTTLASNLAASLAD
                     GGRREVCLVDLDLNFGDVAIVLQLFPAHTLADAVPLLETLDLPAVTALLTPHSPGLSA
                     LVAPVEPGSAETVPASLITTILQLLREEFAFVVVDTPPAFTDHVLAAFDQSDVIALLA
                     TLDIPALKNLKLTLETLDLLNYPRERLRVVLNRADSKVGLALSEVEKTLRVPIALHIP
                     SSRAVPASINRGVPIMLDDPSHPVSMAIRQFGDDYVLPLARAADPIASNLRTDRRGLL
                     RRKSKTS"
     gene            5041..6456
                     /locus_tag="VII50_00720"
     CDS             5041..6456
                     /locus_tag="VII50_00720"
                     /inference="COORDINATES: protein motif:HMM:NF012652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpaF family protein"
                     /protein_id="HEY5049392.1"
                     /translation="MSLADRLAQARRSRDAGADSTTQALEAQASAARRTADPFIDLKR
                     TVHATLLDNLGPQLYDTRFTQADLEQKVRHTLQGVLAQEETPLSVADRTRIAQEIADD
                     ILGYGPLEPFLRDSDVSEIMVNGPSQIYVERAGRLHAVAASFVDEAHLRRTIDKIVAQ
                     VGRRVDETSPMVDARLADGSRINAVVPPIAVDGSLLTIRKFATDPYEADDLIGFGTMT
                     PELAEFLRACVKARLNILVSGGTGAGKTTTLNVLSSFIPSDERIITVEDAAELQLHQE
                     HVLRLEARPPNIEGRGEIRIRDLVRNALRMRPDRIVVGEVRDAAALDMLQAMNTGHDG
                     SISTVHANSPKDVLARIETMAMMAGMELPVRAIREQMASAFDVIVHQARFKDGSRLIT
                     CVTEVVGIAPEGDTVELHDLYLFDHGPGYDDIGRPLGSLRPTGALPNFVAKLAAAGIR
                     VDPVSIGVTAVALPDQADHPS"
     gene            6453..8486
                     /locus_tag="VII50_00725"
     CDS             6453..8486
                     /locus_tag="VII50_00725"
                     /inference="COORDINATES: protein motif:HMM:NF012321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HEY5049393.1"
                     /translation="MKRAGAWATGLTGVAALVLGVGPIALVGTARAASAGTASPAAPS
                     VSPTGRIESQSSKPGQLNVTFSAIGLPAGQSVDLASVRVFLNGTPVDSKATAIGSVTA
                     SAIPEASRAAVLVVDISSSMTGTGLAGAQAAARAFLKAVPPDVMVGLVTVSTTASLVA
                     APTTDRATLAADINGLAATGGTALYDATLLAERAVGTTGSRTIVLLTGSRDDSSKATL
                     AQAVSTAQLSGAVVDAVSLGTSATQVAPLQELTAASGGRLLATQQAADFAPAFRSAAQ
                     DISTEILITAQVPAQFAGLGVTVAVTARAGNTTVTDSAAYQLSSTTNAGSTASKAAAA
                     ASPRPESVTITPLGTHTYLVIGIAAVFVGLLALFVLLAFNAKLLDTRDTRMRRRLSFY
                     TLSGRNTRAEAQPTALGDSSVTRSAVELAGRLVVSRDFDARLGRRLEAAGVPLKPAEW
                     IVVQAGSACGLGVLFLLLGGGSAVAAILGVLIGIGGPIGYLVMRESRRTTAFLEQLPD
                     TLQLIAGSISVGHSLAQAMDAVVREDIQPVSVEFNRALVESRLGMPVEDALEGISTRM
                     VSQDFAWVVMAIRIHREVGGNLAEILTTVAATIRERERLRRQVRGLSAEGRLSAWILG
                     ALPPIFATYLILVRPTYIKPLFTDPLGVTLVGIMIVLMIAGIVWLRRVVRVEV"
     gene            8488..>8938
                     /locus_tag="VII50_00730"
     CDS             8488..>8938
                     /locus_tag="VII50_00730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5049394.1"
                     /translation="MTEQLLLVGGVIAIFAAIVGLVMALGTTASDRQQVNRSLAALDA
                     LGPRRDEVRRELDRPLVERVFGPILHRLSRLGGQLSPRDEASKLRHRIDLAGNPTGWD
                     VERILAFKVIGLVCGIAIGISLPLALNGGLAVTAGVTVLLGPLGYFAP"
BASE COUNT         1383 a         3099 c         2929 g         1527 t
ORIGIN      
        1 gtcgccgacg acggcgagtt gctggtgcgc ggagagcatg tcttcgaccg ctactgggcc
       61 gaccccgatg ccacggcggc cgcacgcgac gccgacggat tcctgcacac cggcgatttg
      121 ggggccatcg acaacgacgg cttcatcacg atcaccggcc gcaagaagga aatccttgtc
      181 acggcaggcg gcaagaacgt gtcgcccgcc ctgctcgaag atcgtttacg cggccatccc
      241 ctaattgcgc aggcaatggt cgtcggcgac cggcgggctt acatcgccgg cttgatcacg
      301 atcgatgagg aagcactcgc cacctggaag accaaggtcg gcaaggaccc atcgctcggt
      361 ctggccgagc tgcgcgaaga ccccgatctg ctggcggaga tccaggcggc cgtcgatgac
      421 gccaaccggg tggtgtcccg tgccgagtcg atccgacgct tccggatcct ggatcgggac
      481 ttcaccgaag ccggcggcca gctcacgccc acgctcaaat tgcgccgcaa tttggtgatg
      541 actgaattcg ccgacgagat cgaaggtttg tattcgccca cggcacccgg cacccggatg
      601 gcaccgtgac atcttggggg atatctacct ggtgacttca gagggatgca aggtcggtcg
      661 agcagacaag cgagatcgcg cgcagccgga ggtgagcagt ggtcgcccgc acacccgcct
      721 acgtcgaaac gatcggcctg cgagtcgcgt tcggacctgt tgtcgcggtc cactcgctgg
      781 acctcacggt gcagcccgcg tcggccgttg ccctcgtggg ccgcaacggt gccggtaagt
      841 cgacgacctt gcgcgtgctg gccggcgtac tcccggccac cagcggcgag gtcatggtcg
      901 ccggggtcga tgtgacccgt gacccggcga ccgccaagtc gcacgtcgga tactgcccgg
      961 acgtcggcgg cctgatcccc cgcgctacgc cgtgggagca ccttgccctg gcggcgaagc
     1021 tgcgcggcct gcgcccggac tggaaggacc gcgcgcatga gttgctcgaa ctcttcgatc
     1081 tcgtgggcgc ggccgatcgg gtcaccgcgg ggttttccca tggcatgggg cgccggcttt
     1141 ccgtcgtgct tgccgtgctg catgagccgt cggtgttgct gctcgacgag cccttcgacg
     1201 gcgtcgaccc gcttggcgtc gacgccacgc ttgaagtgat ttcacgggcc cgcgacaacg
     1261 gctgcgccgt ggtcgtatca acgcacctgc tcgaactcgc cgcagcagtg tgcgacgagg
     1321 tcgtggtgct gcgagcaggc cggatcgtcg cagcgggccc gtcagcggaa ctacgcggcg
     1381 aggcaggcga gctgcgctac cgggcgctac tcgcatgacg ggggcacaac ttcctgccct
     1441 cgttcggttg cgctggcaga tggtcagggc ggaacgcgtc cgcgtcgggc tgctcgtgct
     1501 ggccgcatgc gtacccggcc tgctgtgtgc cgccgtcatc gcgggtcagc ttgcgccgcg
     1561 ctcccgatcg ttcgatgtca ccgtcctcgg accgaccgca ttcctcggct ttgccgtgct
     1621 ctcgctcgtc gcgccgctgg cggccggcgg cggtaacgaa ctgttccctc ccgaacagct
     1681 ggtcgcgtac ccgatccggc cggcgaccca cttcttcgag tcgttgctcg tcgcgccgtt
     1741 gaacctcgcg tggatgagcc agttcgtctt gctgtttggc atgacgtcgt tcgtgaccgg
     1801 cgcccggccc ggcctcggac tcgccctggt cacgactctt gtctacgtcg ccatggtcac
     1861 ggtcgcgggc cagatggtcg cgtggatgtt gatcggtgcc cgccagagcc tgctcggcag
     1921 gcgcgccgtg tgggccctga cggcagccat cgtggtgacg acgctggtcg tcctgcacgc
     1981 gcaccttggc taccgggtcc tcgacaacgc gccgacgaag ccggtcgtgt ctgccgtcct
     2041 cgccggtggt gggcaccgat acttcgcgtg ggcgtcgggg acgactgcgc tcggcatcat
     2101 cttgttgggc tgtctttggt gcgggccgcg gatctgtgcg tgggcacttc gacgcccggg
     2161 cgaccgtacg agattccgag agtcgcgcgt cgagcgccgc cggccgacgc cgccgagccc
     2221 gctggccact ctcgtgcgcg tcgaccgcgc caacgtctgg cgggcagcac cgctgcgccg
     2281 cggcctcctc gtgctggcga tcctgcccgg cgtggtcgcg gccagtgcgg cgctgcggtg
     2341 ggactcactc gtactgcttc ccgcactcgt cgctgcaggc gccggacttt tgtacggcgt
     2401 caacatgttc tgcctcgacg ctggcggcgc cacgtggctc gcctcgctgc cgagtccgcc
     2461 ccggctcgcc gtgaccgcga agacgatcgt cctcggcgag accatcggcg gcagctgcct
     2521 ggtggcggct attgccggca gcgtccgggc ggccggaccg agaacagcag cggacctcgt
     2581 tgccatggcg tgttgcctcg tgtcgtgcac cgccgtcgtc ctggccacct gcctgcggct
     2641 atcgatccga acgccccatc gggcggagct tcggggacac cgtgataccc cagcgccgcc
     2701 cgcggcgatg gctgtctatt cggtgcggct cgcgaccgtc acgaccctca tcggcctgct
     2761 gttctcgttg gccgcacacg agcagtcctt cgctgcctgc ctcgtgctcg cctcgggttt
     2821 gatcgctctc agcggcttgt ccatcgccca aagcttccgc cgctgggatg acccgtcggt
     2881 gcgttcgaga atcgtcgccg ccgtctcgaa cggctgaacc caccccgagc aagatgaccg
     2941 gagtcccgcc gcccccgtag gcttgcggta ccaagtcttc gtgtcccgcg cggccaggcc
     3001 tgcccggccg cccgcagagg tgcgagtatg cgcgtcaagt gtggccgccg ccgggcgcac
     3061 tctcgcacct gaacgacgct cgcacatgaa gggagtggga cgccgaatgg gacgccgcac
     3121 ggtcctgctc gtcgccgccg tcctggtcgc cgccatcgga gccggcttgg tgctgatcta
     3181 cgtccacgac gtgaaccaga aggtcaaggc ccgtgaggcg cccgagcaga tcctggtcgc
     3241 caagaaggtc ataccggcag ggacgacggg ggctgcggcg tcggccgaag gcgacctcga
     3301 gttcctaagg gtcgcgcgcg aagccgtcgc cccgggggcg ctggcgacga tcgacccggt
     3361 cgccaacctg gtcacgttgg cgccgatcta ccccggcgag cagattctga gcctgaagtt
     3421 tggcaagcaa ggcgcgtcga gcctgctgac gatcccgacg gcgggaaccg tggccttgag
     3481 catctcgctg tccgggcctg caggggtcaa cgggttcatc gcgccgggtt cgcaggtagc
     3541 ggtgttcctc accgcgggcg gcgcgacgaa gctgatcctc cctcgcgtct cggtcatcgc
     3601 ggtcggtagc cgcacgctgg tgccgtcgtc gggtcaagcg acgggtacca accagcaatc
     3661 cgacgtggtg acttttggcg tcaccgccgc acaagcgcag acgctgatct acgcgcagtc
     3721 ggctggcagc ctgtatctga ccctgctgac cgcgaattcc tcggtccctt cactgccgcc
     3781 aacgacggca acgaacgtca cgagcggcgc cccgtcatga gggcacagtc atgagcacca
     3841 tgtcatgagc gtcatcgtcg aacccgaccc cacccgagcg gaaacgttgc ggtccgcact
     3901 cgggccgggc gtccttgtcc tgaagacgct tgatgacctt cgccgccacc ttgaagcgca
     3961 ccccggcgag gacgccgtgg tcctcggccc ctctgtcgac caggacgccg ccttccatct
     4021 cagcgacagc atgcggatca ctcgcaccag cctcggcgtg gtcctggtgc gtcggcgcat
     4081 cgacactccc ctgcttgtcg acgcactgcg caacggcatt cgcgaagtcg tcgaggagcg
     4141 caaccttgcg ggagtgaacg acgcggtccg acgggtgcgt gatgtcgcag cggcgatgcg
     4201 tcagcgcgcc tcgcacggcg agggtgaaga cggccgccgc ggagtggtcg tgacggtctt
     4261 ctccgctaag ggcggctgcg gaaagaccac gctggcaagc aatctcgccg cctctctcgc
     4321 cgacggcgga cgacgtgaag tgtgcctcgt cgatctcgac ctgaacttcg gcgacgtcgc
     4381 catcgtgctg caactcttcc cggcgcacac actcgccgac gctgtaccgc tgctcgaaac
     4441 actcgacctg cctgcggtca ccgcattgtt gacaccgcac tcgccgggcc tgtccgcgtt
     4501 ggtcgcgcca gtcgagcctg ggtccgccga gacagtaccg gcgtccctca tcaccacgat
     4561 cctgcagttg ctgcgcgagg aatttgcgtt cgtcgtggtc gacacgccgc ccgcgttcac
     4621 cgaccatgtg ttggcggcgt tcgaccagtc cgacgtgatc gcactgcttg cgactctcga
     4681 catccccgcg ctgaagaatc tcaagctcac cctcgaaacg ctcgacctac tcaactatcc
     4741 ccgcgagcga ctgcgtgtcg tactcaaccg ggccgactcc aaggtcgggc ttgcattgtc
     4801 ggaggtggag aagacgttgc gggtgccgat tgcattgcac atcccgtcgt cgcgcgcagt
     4861 gcctgcctcc atcaaccgtg gagtgccgat catgctcgac gacccgagtc atccggtgag
     4921 catggcgatc cgacagttcg gcgacgacta cgtactgccg ctcgcacgag ctgctgatcc
     4981 gatcgcgtcg aacttgcgaa cggatcgtcg cgggctgttg cggagaaagt cgaagacgtc
     5041 atgagtctcg cggatcggct tgcccaagcc cgccggtcgc gcgatgcggg cgccgactcg
     5101 acgacgcaag cgctcgaggc gcaggcgtcg gccgcgcgcc gcaccgccga tccattcatc
     5161 gatctcaaac gcacggtgca cgccacgttg ctcgacaacc tcggccctca gctctacgac
     5221 acacgattca cccaagccga ccttgagcag aaggtgcggc acacgctcca gggcgtgctg
     5281 gcccaggagg aaaccccact ctcagttgcc gaccgcaccc gcatcgccca ggagatcgcc
     5341 gacgacatcc tcggctatgg cccacttgag ccgttcctgc gcgattccga cgtcagcgag
     5401 atcatggtga acggcccgag ccagatttat gtcgagcggg ccggccggct gcacgccgtc
     5461 gccgcatcgt tcgtcgatga agcgcatctg cggcgcacca tcgacaagat cgtcgcgcag
     5521 gtcgggcgcc gggtggacga gacaagcccg atggtcgacg cccgacttgc cgacggcagc
     5581 cgaatcaatg cggtcgtgcc gccgatcgcc gtcgacgggt cactgctgac gatccgcaag
     5641 ttcgcgacgg atccttacga agccgacgac ttgatcggct tcggcacgat gacgcccgaa
     5701 ctcgccgaat tcttgcgcgc gtgcgtgaag gcacggctca acatcttggt ctctggtggc
     5761 actggggcag ggaaaaccac gaccctcaac gtgctgtcgt cgttcatccc gtcggacgaa
     5821 cgcatcatca ccgtcgagga tgccgctgag ttgcagctgc accaagagca cgtactgcga
     5881 ctcgaagccc ggccgcccaa catcgaaggg cgcggcgaga tccggatccg cgacctcgta
     5941 cgcaatgccc tgcgcatgcg ccccgatcgc atcgtcgtcg gcgaggtgcg cgatgccgca
     6001 gcactcgaca tgctgcaggc aatgaacacc ggccatgacg ggtcgatcag cacggtgcac
     6061 gccaactcgc cgaaggacgt gctcgcccgc atcgaaacga tggcgatgat ggccggcatg
     6121 gaactgccgg tacgcgcgat ccgcgagcag atggcgagcg cgttcgacgt catcgtgcac
     6181 caagcaaggt tcaaggacgg cagccgactg atcacctgcg tgaccgaggt tgtcggcatc
     6241 gcgcccgagg gcgacactgt cgagctgcac gacctgtacc tgttcgacca cggccccggg
     6301 tacgacgaca tcgggcgccc gcttggatca ctgcggccga ccggcgcgct gcccaacttc
     6361 gtcgccaaac tcgctgccgc aggtattcgt gttgaccccg tctcgatcgg agtgaccgcc
     6421 gtcgccctcc cggatcaggc ggaccacccg tcatgaagcg cgccggcgcg tgggcgaccg
     6481 gactgaccgg tgtcgcggcg ctggtgctgg gtgtggggcc aatcgcactc gttggcaccg
     6541 cacgcgctgc tagtgcaggg acggcgagcc cggcggcccc gtcggtgtcg ccgaccggac
     6601 gcattgaaag ccagtcgagc aagcccggcc aactcaacgt cacgttcagc gcgatcgggc
     6661 tgccggctgg ccagtccgtc gacctcgcat ccgttcgcgt cttcctcaac ggcacgcccg
     6721 ttgacagcaa ggccacggcg atcgggagtg tgaccgcgag cgcgatcccc gaagccagtc
     6781 gcgccgcggt gctcgttgtc gatatcagca gcagcatgac gggcaccggc ctcgcgggcg
     6841 cgcaagctgc cgcgcgggcg tttctcaagg ccgtgccgcc cgacgtgatg gtcggcctcg
     6901 tcacggtctc gacaaccgca tcgctcgtcg cggcgccgac aaccgaccgc gcaacgcttg
     6961 cggccgacat caacgggctc gccgcgaccg gcggcaccgc gctgtacgac gcgaccctgc
     7021 tggccgaaag ggcggtcggg acgacagggt cgcgcaccat cgtcttgctg accggtagcc
     7081 gcgacgattc gagcaaggcg actctcgccc aggcggtctc gacggctcag ctgtccgggg
     7141 ccgtcgtcga cgcggtgtcc ctcggcacat ccgcaaccca ggtcgcgccg ctccaggagt
     7201 tgacggcggc ctcgggtggc cggctgctcg ccactcagca ggccgccgat ttcgcgccag
     7261 cgtttcgcag cgcagcgcag gacatctcca ccgagatcct gatcaccgca caagttccgg
     7321 cgcagttcgc gggcctgggt gtcacggtgg cggtcaccgc acgggccggc aacacgaccg
     7381 tcaccgactc ggccgcctac cagctttcga gcaccaccaa tgctggtagc accgcgtcga
     7441 aagcggcggc agcagcgagc ccacgaccag agagcgtgac gatcactccg ctaggcacgc
     7501 acacctacct ggtgatcggc attgccgcgg tcttcgtggg gttgctcgcg ctgttcgtcc
     7561 tgctggcgtt caacgcgaag ctgctcgaca ctcgcgacac gagaatgcgc cgccgcctgt
     7621 cgttctacac gctcagcggc cggaacaccc gggcggaggc gcaaccgacg gcgttgggtg
     7681 acagctcagt gacgcgctcg gcggtcgaac tcgccggccg gctggtggtg tcccgcgact
     7741 tcgatgcacg cctgggccgc cgcctcgagg ctgcgggggt gccgctcaaa ccggccgaat
     7801 ggatcgtcgt gcaggccgga tctgcctgcg gcctcggtgt gctcttcctg ttgctaggcg
     7861 gtggatccgc cgtcgcggcc attctcggcg tgctgatcgg catcggcgga ccgatcggct
     7921 acctcgtcat gagggaaagc cgtcgcacca ccgcgttcct tgaacagttg cccgacacgc
     7981 tgcaactcat cgccggcagc atctcggtcg gccactcgct cgcgcaggcg atggatgccg
     8041 tcgtccgcga agacattcaa ccggtatccg tcgagttcaa tcgagcgttg gtcgagtcgc
     8101 gcctcggcat gcccgtcgag gacgctctcg aagggatatc gacacggatg gtgagtcagg
     8161 acttcgcgtg ggtcgtgatg gcgatccgaa tccatcgcga ggtcggcggg aacctggccg
     8221 agatcctgac gaccgttgcg gccaccatcc gcgagcgcga gcgactacgt cgacaggtcc
     8281 gaggactgtc ggccgaaggc aggctgtcgg cctggatctt gggggcgctc ccaccgatct
     8341 tcgccacgta cctgattctg gtgcgcccga catatatcaa gccgctcttc actgacccgc
     8401 ttggcgtcac gctggtcggg atcatgatcg tcttgatgat tgccggcatc gtctggctgc
     8461 gccgcgtcgt gagagtcgag gtctgagatg accgagcagc tcctgctcgt gggtggggtc
     8521 atcgccatct tcgccgcgat cgttggcctc gtgatggcgc tcggcacaac ggcgtcggac
     8581 cgtcagcagg tgaaccgatc cctggcggcc ctcgacgcac tcggtccgcg gcgcgacgag
     8641 gtgcggcgcg aactcgaccg cccattggtc gagcgggtgt tcggcccgat cctgcaccgc
     8701 ctcagccggc tcggcggcca gctcagcccg cgcgatgagg cgtcgaagct gcgtcaccgc
     8761 atcgacctcg ccggaaaccc aacgggttgg gacgtcgaac gcatcttggc tttcaaggtc
     8821 atcggcctgg tctgcggaat cgcgatcggg atttccttgc ccctggcatt gaacggcggc
     8881 cttgcagtca ctgcaggcgt caccgtcttg ttaggcccgc tcggctactt cgcgcccg
//
LOCUS       DATCBJ010000036        12760 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_400053, whole genome shotgun sequence.
ACCESSION   DATCBJ010000036 DATCBJ010000000
VERSION     DATCBJ010000036.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 12760)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12760)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12760
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_400053"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            201..791
                     /locus_tag="VII50_00735"
     CDS             201..791
                     /locus_tag="VII50_00735"
                     /inference="COORDINATES: protein motif:HMM:NF024273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEY5049395.1"
                     /translation="MSRPGVPGLELGADNGAQEVVSRMDPDKAAVHEELERVRQVFAR
                     HIDDMTPGALDRISDGTRWTNRQLLFHMLFGYIIVRRLIWMVKLLSYLPAAATKPFAA
                     LLNAATRPFNWANYIGPVFAARIISPARMQHWLRGLTVWLEKDLNRQNAKGLTRGMYY
                     PTKWDPYFKRYMTLADLYRYPTQHFDHHDRQLSAPQ"
     gene            complement(1524..1877)
                     /locus_tag="VII50_00740"
     CDS             complement(1524..1877)
                     /locus_tag="VII50_00740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5049396.1"
                     /translation="MDGEWLDDAFGPDGVRVTFCFTTVVSRYAQALSGIGIVGSMVTG
                     ESRVGPSPWRRFRPQLLHGLERVVAGYVLAIEADGSKPFYVRRQTRDAIIEFSNSAHA
                     VVVAGGVSALANFRS"
     gene            complement(1967..2377)
                     /locus_tag="VII50_00745"
     CDS             complement(1967..2377)
                     /locus_tag="VII50_00745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5049397.1"
                     /translation="MPAAGITPDVPAAGAVATITPTQAYVDSCTTDGVCDKGSAPTIQ
                     LAVVTDPNSGQAAAGGSLTPVMDHTLAYVLTWTNVPCAPAGAPVGATPTPTTCTLVDF
                     VDANTGKTLYGLDKSGPNDLSPVTSQSPATTTKP"
     gene            3153..3464
                     /locus_tag="VII50_00750"
     CDS             3153..3464
                     /locus_tag="VII50_00750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5049398.1"
                     /translation="MCPGEASIGDRVTITSDTMCGAQPNPPAALVFLGSKSYMGSGGG
                     GVDVRAITRHGSGFAVSFVIPKTYVSGDPVNAEVPVTPGAYFFTTLPISTCYVQLQVV
                     G"
     gene            3742..4257
                     /locus_tag="VII50_00755"
     CDS             3742..4257
                     /locus_tag="VII50_00755"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEY5049399.1"
                     /translation="MTTFDGFYAKDRALLGRYLLVAGCPRDAVEDVAQEAYGRALKHW
                     SDIGDRNPEAWVRVVATRIWFDEYRRSVKQHAVGDRLRLVGPTARDSHAEASTMKVDV
                     GRALERLPAHQRAVVVLFYLTDASVGEIATTLGWTRAKVKVDLFRGRRRLLAVLATYG
                     AAPPVKEDHHA"
     gene            complement(4345..4695)
                     /locus_tag="VII50_00760"
     CDS             complement(4345..4695)
                     /locus_tag="VII50_00760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5049400.1"
                     /translation="MITRTWLFHADPPTICVPDGTGLEVGGAEATAGGLVSCAEVDGV
                     GVGWADGVGTAVGVGATEAGDGTTDGVAGEGVLLNTGSTAGARPSWRDCAKAHVPTDK
                     ATIATTTQMAAARH"
     gene            4727..5164
                     /locus_tag="VII50_00765"
     CDS             4727..5164
                     /locus_tag="VII50_00765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5049401.1"
                     /translation="MSSAQAARAARVSAFTPVGDGVLVPAEQANSPQQLYLYLSSFAG
                     QPPASFRGDFLCVAADGSQTATQSVVTAAGLHLGDSAARLREIYGAAATFIPEPTGGI
                     EPHPGYVVHQGQFDLVFKLDPSLSRVIAIAGGIAPMTPSQCNG"
     gene            5339..6199
                     /locus_tag="VII50_00770"
     CDS             5339..6199
                     /locus_tag="VII50_00770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5049402.1"
                     /translation="MAAMPLRVLCGVTLLLLVGCRASGRQPAASEPTTLASVGGSTSS
                     ASVSAPAGSVSSRPSTPISASSPTASTPAIAPATPSQTRPADTSTPASPGTGHATASP
                     GGKPATPSPGGNPLVDEIWGNNAVPFRPSTSADYLHTPRVQQSFGGQAASLVPANWKY
                     VDETIPSDHNDLMWYDPANPAARIEFTGTGCESCVLQNVDATHPVRDVVRGLPFDTTS
                     YDVYNHGLDAGFRETDADGYAVNGVVALIGTDADPNGYVTYRVSLPADDTPLATRILN
                     SFTGLSQGTR"
     gene            complement(6180..6449)
                     /locus_tag="VII50_00775"
     CDS             complement(6180..6449)
                     /locus_tag="VII50_00775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5049403.1"
                     /translation="MVGLALAAGLAVTVVDATGAGRAEAIADSGGIDEVAAVDAVNGA
                     LEGPDLCAWVVGVQLTSRPTHPVSATNLNAPTTAPLSSTIIWSLG"
     gene            6508..7956
                     /locus_tag="VII50_00780"
     CDS             6508..7956
                     /locus_tag="VII50_00780"
                     /inference="COORDINATES: protein motif:HMM:NF019151.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1524 domain-containing protein"
                     /protein_id="HEY5049404.1"
                     /translation="MTGYNPAQFGPAWPELAGCDALNSVLARDLTKTRLQGSCIVKSG
                     ILVSPYSGATINFGSNPGLVTIDHVVALGDAWQTGAQTWTAAKREIFANDLLELLATD
                     AASAGQKGDADAATWLPSNKTFQCDYVETQVDVKTRYGLWVTQVEHDAIARVLASCLT
                     PAPVAAAVFQFLPATYRVTSTTPVDLDGSDTPQVVVTAIGTIQDGGSSGLAPTTVLLL
                     TFDPLANRWTEAFDASQEPSYQANSQQGAGPGVVSPTGSGPQIALVHDQPAGGADLLY
                     WVNSVGGNSGKLIVGIVHFASGVATLTYSEDGDEGHVGSFDVPTSTPAAVSVTGTGPN
                     QTVQITLPWITAADSRSQAARMYTRTLAPNWTGYYQVIADDRPYVGVAISPLPGRTQA
                     RVESVDPTSPANGELHVGDILTGVADTALGTTITSTLIGPPVIDEVAILQPETTVTLQ
                     IVRNGTSSDVTLRLAHWSAAADALTKVGPTAL"
     gene            complement(8715..9038)
                     /locus_tag="VII50_00785"
     CDS             complement(8715..9038)
                     /locus_tag="VII50_00785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5049405.1"
                     /translation="MGAGPTNAGGGTEDGEKVATNVLAPTGPGGLLTGPDGLLLTGAD
                     TLGLTVAMPGDETAAVGSAPNGLPAECGFLPATASQVTPLASTTTTATAITSDRRGRG
                     HRAGS"
     gene            9061..10029
                     /locus_tag="VII50_00790"
     CDS             9061..10029
                     /locus_tag="VII50_00790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5049406.1"
                     /translation="MTSNTAFGGVGIFALDGGAISTVRGFPAGGCPVVRAFRIPGTEQ
                     WAVVWQPADNASAPCGLGPGQLYIIDAARSTAHLVGSEQGVVAADRGSVWTVTGVDLL
                     PGQQLSVPEQVQRISLTGAVLSRVYSLPLGWTVTKGLTPDLLLLARDLANGLDNWEAW
                     QPSTGTVLGQYERVLAANASVVAWVNNTCAPDNCPIHLSPPSGGADRTVSLPAGAYAY
                     DGSLSDDGTYLALSLSTGRDTQGATDQDTGVLVDMASRAVHPIQQTKIPAGETGSLSL
                     NFGPNGWVIVSTPGPNMTSQLAAYNPTTNSFVVCRHLPPADESMAF"
     gene            10178..10918
                     /locus_tag="VII50_00795"
     CDS             10178..10918
                     /locus_tag="VII50_00795"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HEY5049407.1"
                     /translation="MNEESLGGNVTAAVRVGNTVRRPVGPWTDAVDALLEHLAAVGFT
                     GAPKALGRDEQGRQVLEFVPGGVGTPSPDYSLSELHQIGHLLRDLHTATASFTAPAMA
                     EWNQVIVPDRQELICHNDPAPWNLVKAADRWVFIDWDFAGPGSRLWDLAYNAQTSVPL
                     QAGLEVSAVAPRLRALVDGYDLDGTGRIALAGMLARRARAMHEMLQRRASAGEAPWSR
                     IWIEDGPYWDETADYLATNSAAWADALL"
     gene            complement(11153..11593)
                     /locus_tag="VII50_00800"
     CDS             complement(11153..11593)
                     /locus_tag="VII50_00800"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEY5049408.1"
                     /translation="MPPIIVSADVGRSADDVYAYTTDPSRFGEWQQGVVRGHMELAAT
                     AEKPRCVTVRRIGFAERASTSEVVRADPPRAWSVRGIDGPIRALVNVSVTPLTHVRSR
                     VTISVDFEGHGVGKLLVPLAVRRQARKEMPTSVATLKRRLESES"
     gene            complement(11594..12076)
                     /locus_tag="VII50_00805"
     CDS             complement(11594..12076)
                     /locus_tag="VII50_00805"
                     /inference="COORDINATES: protein motif:HMM:NF019928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEY5049409.1"
                     /translation="MTQADSAVVRTHIVVEVSAEKAFSVFTERLGDFKPPEHNLLAAP
                     ITETVFEPRVGGHIYDRATDGTECHWARVLVYEPPLRVVFSWDIGPTWQLETDPENAS
                     EVEVRFIAETADRTRVELEHRNIDRHGAGWEAVADGVGNDEGWPLYLARYSALFGEGS
                     "
     gene            complement(12073..12411)
                     /locus_tag="VII50_00810"
     CDS             complement(12073..12411)
                     /locus_tag="VII50_00810"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEY5049410.1"
                     /translation="MVAYGSEGAWAALGDPTRHAIVVALAERPRAVTELAAELPVSRP
                     AVSQALKVLKDAGLVTERAAGTRRIYRLNPTAVAALRDQLDTFWSRALANFTDTVEQP
                     SAERPRQESS"
BASE COUNT         2277 a         4346 c         3970 g         2167 t
ORIGIN      
        1 cacgcggctg cgccgagaaa cgctgtcacc gcgtagatcg tccaataacg tcgcgaccgt
       61 ccttgaccgg tgtaccacat tgttcctctg tacccggcga gcgacctccc gacccacatt
      121 gacgcctcgc acgtgcgcgt ctcagcggca tgtgagtgtg tcggtgacag gcaacgaggc
      181 catacggccg accgtgtttg gtgagtcgcc ccggtgtgcc cggacttgaa ctgggcgccg
      241 acaatggcgc ccaggaggtg gtgtcacgta tggacccgga caaggcggcg gtgcacgagg
      301 aactcgaacg ggttcgtcag gttttcgctc ggcacatcga cgacatgacg cccggcgccc
      361 tcgaccggat cagcgacggc accagatgga ccaaccggca actgctgttc cacatgctct
      421 tcggctacat catcgtccgg cgcctgatct ggatggtgaa gttgctgagc tatctgccgg
      481 ccgccgccac caagcccttc gcagccctgc tcaacgccgc gacgaggccg ttcaactggg
      541 ccaactacat cggccctgtc tttgccgcaa gaatcatctc accggctcgg atgcagcact
      601 ggctccgtgg gcttacggtc tggctggaga aggacttgaa ccggcagaac gccaagggtt
      661 tgactcgcgg gatgtactac ccgacaaagt gggacccgta cttcaagcga tacatgacac
      721 tggcggacct ctaccgctac ccgacacaac acttcgacca tcacgaccgc caactcagtg
      781 ccccgcaata gctgggacgc tcctatccca cctcgccgcg aaaccagccg aatcgtcgaa
      841 cgaccgtcat ggcggcaaat gatcgcgttc agctgacgcg ggtccgcggc acggcggact
      901 cagatcagcg cggttggtcg agttcgatta gagccctttg cgccgcagcc ctcgcagcag
      961 accgatcggg cgccttcccc agctcacgcg accacgaacg gctactgacc agtaccagcc
     1021 gcgcctcggc cctcgccatc gaactttgac gcgcgcattc agctcccgag gatggcacta
     1081 acggcagcac gaccgcacgc tcgtcaaagc gcaaatgagg atgtccaact tcgcaaggcc
     1141 ggtaccgttc aatcgtgcga atcacttacg acccagaggc caacgccgcc tacatctatt
     1201 tcaccgacga cccgcttatg cccggtcgag acagcatgcc gctccaagac gttctaagtg
     1261 agcgcggcga gatggtcgtc atggattgga aggacggcaa gatcgtcggg ctggaagttc
     1321 tcggggcatc gtcgtttgct gcatgccgac ctactcgctc aggccgttcg gccagggaag
     1381 cattaggcag cccatcgctt aggacaatta ccggatctca acacgacaga aacggccatc
     1441 aaggcggcaa gacagtgcga atcttgcagg tcgtcaggct tcgtaaggtc tctatggttc
     1501 tggactttcg cgcacggccg cacctatgac cggaagttgg cgagggccga aacaccgcct
     1561 gctaccacga ctgcgtgagc gctgttggag aactcgatga tcgcgtcgcg tgtttgccga
     1621 cgtacataga acggtttcga gccgtctgct tcgatggcca acacgtaccc ggctactacc
     1681 ctttcgaggc cgtggagaag ttgtggacgg aaccgtcgcc agggtgacgg gccaacgcga
     1741 gactcacccg tgaccattga tccgactatc ccaatcccgc tcaacgcctg ggcgtagcgg
     1801 gacacaaccg tcgtgaagca gaaagtcacg cgcaccccgt ccggtccaaa cgcatcgtcg
     1861 agccattcgc cgtccatgtg cctatcctcc cgcgacggtt cgaacgtgcg tcatcgcggc
     1921 aaatgtgcgc gtttcgctcc ggcgtcagtt gtggcgcgca agctcatcaa ggcttcgtag
     1981 tagtcgctgg gctttggctc gtaacagggc tgagatcgtt gggcccgctc ttgtctagtc
     2041 cgtaaagagt cttgccggtg ttcgcgtcta cgaagtccac cagggtgcag gtcgttggcg
     2101 tcggtgtggc gccaacgggc gcgccggctg gtgcgcaggg gacgttcgtc cacgtcaaca
     2161 cgtaggccag cgtgtggtcc atgaccgggg taagtgagcc gccggctgcg gcctgcccac
     2221 tattggggtc tgttacgacg gcgagttgga tggtcggcgc gctgcctttg tcacatacgc
     2281 cgtcagttgt gcagctgtcg acgtaggcct gggtcggcgt aatcgtggcc acggcgcccg
     2341 ctgcgggaac gtcgggagta attccggccg cgggcatgat ctctggaacc gtcagcctcc
     2401 tgaagcttgt agcgggcgcg aacccaggcg gcggtgggcc taacgagctc attaaggcga
     2461 ccaccgtggg cgtagttgca gtcggcggac cggaagcagt ggtatctgcg accgtccgac
     2521 aggcgcaccc ggcaacgccg acaagcaccg ccgctgcgca gatcaacagc cgggtgcggc
     2581 ttccagcaat gctgatcacc tacctaggat gcgccagctg tgagtgcggt tcctgaacac
     2641 tcgattgact cccagcgagc accgccagcc agatgagcag ccattgctcc cacccccact
     2701 cgcacgaccg aacgcgcgtc aacgcggcat gaccgtgcga ccggtatgcc caccgtcggc
     2761 ggcatgaccg gatgtgctgt gcccgcgtca ggaatcattt gatcgatcat taggacgtcg
     2821 gtccgtacgc cgcgcttctg ccttgcgcgt ccaattgagg tggcgctctc tgcggatcag
     2881 gggcgacagg tgaaggaggc agaccgatga cccgaactcg ttggacgcgc gatcttgttg
     2941 tcgtggtggc tgcggccgcc ctcgcggtgg cgggatgttc gagttccggt ggctccggcg
     3001 tgcgatcctc gccacaggag acgagttctt cttcgtcgca gtcggtctcg atcccggtca
     3061 gcagtccggc tatcaccccg tctaacccaa taacgacctc gcctgcaacg ccagcctgtg
     3121 tttcgaagag cagcgtcgat gggaccctga cgatgtgtcc cggggaggca tccatcggtg
     3181 atcgagtcac gatcacctca gacacgatgt gcggtgccca gcccaaccct ccggcagcgc
     3241 tcgtgttcct cggctcgaag tcctacatgg gttccggtgg cggcggtgtg gacgtcaggg
     3301 cgatcactcg acacgggtcg ggcttcgcag tgagcttcgt cattccaaag acctacgtct
     3361 ccggtgatcc cgtcaacgcc gaggtcccgg ttactcccgg cgcatacttc ttcaccacgc
     3421 tgccaatctc cacctgctat gtgcagctcc aagtcgtggg ctgacggcgt cccggcggca
     3481 gatctgtccg gcgcccgcga cgagccgatc ccgtgtcggg cctgcaggtt tcctccattc
     3541 ggcgggtgat tcacgtaggc gatcgaatcg ctgatcgcgc accgcggccg cgatcgagct
     3601 cgacccgagc acgatgtggc ggccacggtt gacgatgcgc cggaaacgcg ttggcggcac
     3661 gttctctgaa agatcttgta accggatggc ctacggtcac gtagtcgtag tgtgaatgcc
     3721 gcgaccgacc gcaaggaagc cgtgactacc ttcgatgggt tctacgccaa agaccgggcg
     3781 ctgcttggtc gctatctgtt ggtagccgga tgtccgcggg acgccgtcga ggacgttgcg
     3841 caggaagcgt acggccgtgc gctcaagcat tggtcggaca tcggcgaccg gaaccccgag
     3901 gcctgggtgc gtgtcgtggc cacccgcatc tggttcgacg aataccgccg atcggtcaag
     3961 cagcacgcgg tgggcgatcg tctacggctc gtcggcccca cagcccggga ctcgcacgct
     4021 gaggcgtcga ccatgaaggt cgacgtcggg cgtgcgctgg agcgcttgcc cgctcaccag
     4081 cgagctgtcg tcgtgctgtt ctacctgacg gacgcatccg tcggcgaaat cgcgaccaca
     4141 ttgggttgga cgcgggcgaa ggtcaaggtt gacttgttcc gtggccggcg tcggttgcta
     4201 gctgtcctcg cgacgtacgg cgcggcccca ccggtgaagg aagaccacca tgcgtgactt
     4261 cgagcgtgca gaagacaacc gactggcagc cgcgctgcaa cgcctcgacc ccgactcttt
     4321 ggcgcccgcc ccgccgccga ccagtcagtg gcgcgcagcg gccatctgcg tcgtcgtcgc
     4381 gatggttgcc ttgtcagttg gcacgtgggc ttttgcgcag tcgcgccacg atggtcgcgc
     4441 acccgcagtg gaacccgtgt tgagcaacac gccctcacca gctaccccgt ccgtcgtgcc
     4501 atcgccagcc tcagtcgcgc cgacgccgac ggcggtgccg acgccgtccg cccagccgac
     4561 gccaacgccg tcgacttctg cgcaactgac gagcccgccc gcggtggcct ctgcaccccc
     4621 cacctcgagc ccggtgccgt ccgggacgca gattgtgggc gggtctgcgt ggaacagcca
     4681 cgtccttgtt atcaccggcc actctttggg cggcgtgagc atcggaatgt ctagcgccca
     4741 agcggcccgc gcggcccgcg tttcggcgtt caccccagtc ggcgacggcg tgctcgtacc
     4801 cgccgaacag gcgaacagcc cgcagcagct ttacctctac ttgagcagct tcgccggtca
     4861 acccccggcg tctttccgcg gggacttcct ctgcgtcgcc gccgatggat cgcagaccgc
     4921 gacacagtcc gtcgtgaccg ctgcgggact gcatctgggc gactccgcag ctcggctgcg
     4981 cgaaatctac ggtgcggccg ccaccttcat cccggagccc accggaggca tcgagcccca
     5041 ccccggctac gtcgtccacc aaggacaatt cgacctcgtc ttcaaactcg acccgtccct
     5101 atcgcgcgtc atcgccatcg ccggcggcat cgcgccgatg acgcccagcc aatgcaacgg
     5161 ctgaatgcca cctggtgtgc gcggcgcccc ctgctgctag gttcacacgc gcgtcaaagc
     5221 gacaattgcg gatgcgtctg gctcagacca cgaccgcatc ggacgtactc agtcaacctg
     5281 cagcggtgct gatgcgtctc atgatgagag atcttccgca cagcagaacg gagatcggat
     5341 ggccgcgatg ccgctgcggg tgttgtgtgg tgtgacgttg ctgctgttag tcggatgtcg
     5401 cgccagcggt cgccaacctg cggcttccga accgaccact cttgcgtcgg tgggcggctc
     5461 aacctcgtcg gcttcggttt cggcgcccgc gggctcggtc tcgtcccggc cctccacgcc
     5521 aatcagcgcg tccagtccca ctgccagcac cccggctatc gcgcctgcga cgcccagcca
     5581 gacgcgacca gcagacacct caacgcccgc gtcgcccggc accgggcatg caaccgcaag
     5641 ccccggcgga aagcctgcaa ccccaagccc gggcggcaat ccgcttgtcg atgagatctg
     5701 gggcaacaac gccgtgccgt ttcggccgtc aacatctgcc gactacctgc acaccccgcg
     5761 tgttcaacaa tctttcggag gccaagccgc aagcctggta ccggcgaact ggaaatacgt
     5821 cgacgaaacg atcccctccg accacaacga cctcatgtgg tacgacccgg cgaacccggc
     5881 ggcgcggatc gagttcaccg gcaccggctg cgaatcctgt gtccttcaaa acgtcgatgc
     5941 cacgcacccc gttcgcgacg tcgtgcgcgg actgccgttc gacacgacca gctacgacgt
     6001 gtacaaccac ggcctcgacg cgggcttccg cgaaaccgac gccgacggat acgccgtcaa
     6061 cggcgtcgtc gcactgatcg gcacggacgc cgacccaaac ggctacgtca cctaccgcgt
     6121 ctcgctccct gccgacgaca ctccgctcgc cacccgcatc ctcaacagct tcaccggact
     6181 cagccaaggg accagatgat ggtcgaggac aagggagctg ttgtgggggc gttgaggttc
     6241 gtcgcgctga cgggatgcgt tggcctgctc gtcagctgca cgccgaccac ccaggcacac
     6301 aagtcggggc cttcgagcgc accgttgacg gcatcgacgg ccgcgacctc gtcgatgcca
     6361 ccgctatccg cgatggcctc agcgcgccca gcgccagtgg cgtcgacgac agtcaccgcc
     6421 agcccggctg ccaatgccag cccaaccacg tttgccgcca atgagctcgc gtcactggcg
     6481 cggctgccga tcagggcggc tgcaccgatg accggctaca acccggcgca gttcggcccc
     6541 gcgtggcctg agctcgccgg ctgcgacgcc ctcaatagcg tgctggcccg cgacctgacc
     6601 aagaccaggc tgcagggcag ctgcatcgtc aaaagcggaa tacttgtcag cccctacagc
     6661 ggggcgacca tcaacttcgg gtcgaacccc ggtctcgtga cgatcgatca cgtggtcgcg
     6721 ctcggtgatg cgtggcagac cggcgcgcaa acctggaccg ctgcgaaacg cgagattttc
     6781 gccaacgacc tcctcgagct gctcgccacc gacgcggcaa gtgccgggca gaaaggcgac
     6841 gcggacgcgg cgacctggct ccccagcaac aaaacgttcc aatgcgacta cgtcgagacc
     6901 caggtcgacg tcaaaaccag atacggcctg tgggtcaccc aggtcgaaca cgacgcgatc
     6961 gcgcgcgtgc tggccagctg cctcaccccg gcaccggtag ccgcggctgt cttccagttc
     7021 ctgcccgcca cctatcgggt gacgagcacg acaccggtcg acctcgacgg atccgacacc
     7081 ccgcaagtgg tcgtcactgc gatcggcacg atccaagacg ggggctcgtc cgggttggca
     7141 cccacgacag tgctgctgct gaccttcgac ccattggcga atcgctggac tgaagcattc
     7201 gacgcctccc aagaaccgtc ctaccaagcc aactcccagc aaggcgccgg accgggcgtg
     7261 gttagtccga ccggctcggg cccgcaaatc gcactcgtcc acgatcagcc agctggcgga
     7321 gccgacctgc tgtattgggt gaactctgtc ggcgggaaca gcggcaaact catcgtcggc
     7381 atcgtgcatt tcgccagtgg cgtcgccacc ctgacctaca gcgaggacgg cgacgaaggc
     7441 cacgtcggca gcttcgacgt accgaccagc accccagctg cggtcagcgt caccggtacc
     7501 ggcccgaacc agacggtgca gatcaccctg ccgtggatca ccgccgcaga cagcagatcg
     7561 caagccgccc gaatgtacac acgaacactc gcgcccaact ggaccggcta ctaccaggtc
     7621 atcgccgacg accggcctta cgtcggtgtc gcgatctcac cgcttcctgg ccgcacccaa
     7681 gcgcgcgtcg aatccgtcga ccccacctca cccgccaacg gcgaactgca cgtcggggac
     7741 atcctcaccg gcgtagcaga caccgcactc ggcaccacca tcaccagcac cctcatcggg
     7801 ccaccagtca tcgacgaggt cgccatcctg cagcccgaaa ccaccgtcac cctgcaaatc
     7861 gttcgcaacg gcacgtcgag cgacgtgacg ctgcgactgg ctcactggag cgccgccgct
     7921 gacgcactca ccaaagtcgg gccaaccgcc ctgtgacagc cgcacgaccc cgacgcagac
     7981 atcggcacga ccgaacgcgc gtcgcggcat gttagtgcgt tgcgcgaggg ctatctcact
     8041 gagcagacca cgtcggacgc gacagcttga ccggcggcga gactcaccgt gtcggcggtc
     8101 gttcgacagc cacctggact gttggtgtcc tgccccatcg cgccagtaac cgtgtacgtc
     8161 ccgacaggaa gcactgcggc gtacttgcca tctgatccga ccgttacatg tcgagatacc
     8221 gggccatcga tgttcactga cccgggaaca ggacgtgcca accccggcgc aggcccgcca
     8281 acctctatga ggcgtccagt aagtgttgcg ttgctggctg gcgaggatgt cggcgtggtc
     8341 ggacccgcgt gcgtgctagt acacgccgca gccagactgg tcgcggcgag ggcgagcatc
     8401 acaccgcgga acatgggcac gacgatacga tgccagcgat gggctccgcg ttgcacgatc
     8461 agccatctag gcatccaccg accactgtct gggatgcctc gagtcgaggc agcatgccgt
     8521 cgggctcacc cgccaaccga ccctctcggc ggcattatcg ggcgatgtga cctgctctac
     8581 ctgagtgaat gctttggtcg gtgaagcctg tggtcgcgac ttctcgccgg atatccggtg
     8641 cgcatacagt cgctgggaca atcgcagcaa agcggcctga ggatagaggt cactgccatc
     8701 ggcgacgttg taggtcacga cccggcgcgg tggcctcggc cacggcgatc gctggtgatc
     8761 gcggtggccg tggtggttgt gctggccagc ggcgtgactt ggctcgccgt ggcaggaaga
     8821 aaaccgcact ctgcgggcaa gccgttcggt gcgctgccga ctgcggcggt ttcgtcaccg
     8881 ggcatcgcga cggtaagccc gagcgtgtct gcgccagtga gcagcagccc atccggaccg
     8941 gtaagcagcc cgcccggacc ggtgggcgcg agcacgttcg tggcgacctt ctcaccgtct
     9001 tctgtgccgc cgccggcgtt ggtgggtccg gctcccacaa acaccggtat ccggatgctc
     9061 gtgacgtcca acaccgcgtt tgggggtgtc gggatctttg ccctcgacgg tggggcgata
     9121 tcaacagtgc gcggcttccc cgcgggtggc tgcccggtgg tccgcgcctt tcggataccg
     9181 ggcacggagc aatgggcagt ggtctggcaa cccgccgaca atgcgtcggc gccgtgcgga
     9241 ttgggacccg gccagctgta catcatcgac gccgccaggt cgacggcaca cctggtcggg
     9301 tcagaacagg gtgtcgttgc tgctgatcga ggctcggtgt ggaccgtcac gggcgtcgat
     9361 ctgctgcccg gccagcaact ctcggtcccc gaacaggtgc aacggatcag cctgaccggt
     9421 gcagttctca gccgcgtcta ctccctgccg ttggggtgga cggtgaccaa aggactcacc
     9481 cctgacctgc tgcttctcgc ccgcgacctg gcaaacggac tcgacaactg ggaggcgtgg
     9541 caacccagca caggcactgt tctcggtcaa tacgagcggg tgctcgcggc gaacgcaagc
     9601 gtagtcgcct gggtgaacaa cacctgtgcg ccggacaact gccccattca cctcagccca
     9661 ccatcgggtg gtgctgaccg cacagtctcc ttgcccgcag gcgcgtatgc ctacgacggt
     9721 tcgctcagcg acgacggcac ctacctcgcg ctatcgctta gtaccggacg cgacacccaa
     9781 ggggccaccg accaagacac cggcgtcctg gtcgacatgg cttcgcgagc cgtccacccg
     9841 atccagcaga ccaaaattcc cgccggtgaa acggggagcc tgtcactgaa cttcggaccg
     9901 aacggatggg tcatcgtcag cacccctggg cccaacatga ccagccaact cgccgcatac
     9961 aacccaacca ccaacagctt cgtggtctgc cgacaccttc cgcccgcgga cgaatccatg
    10021 gccttctagc gcacctctgc ccgcggcggc accacggtct tggcagacga ttcccgacgt
    10081 catcgatgct cgacaccgcg cgacgactcg cgcgcatggc ggcaagacag tgcgtttccg
    10141 gtggcgagct gaacaccggg gcggcgacac tgtctgagtg aatgaggagt cgctgggcgg
    10201 gaacgtcact gccgcggtgc gggtcggcaa caccgtgcgt cgaccagttg gaccatggac
    10261 agatgccgtg gacgcgctac tcgaacacct ggccgcggtt ggattcactg gggcaccgaa
    10321 ggctttgggt agagacgagc aggggcgtca ggtcctggag tttgtgcccg gcggagttgg
    10381 gacgccctcg ccggactact cgctgagcga gctgcaccag atcggccacc tcctacgcga
    10441 cctgcacacc gcaaccgctt cgttcacagc tccggcgatg gcggaatgga atcaggtgat
    10501 cgtgcccgac cgccaggagt tgatctgcca caacgaccca gcgccatgga acctcgtgaa
    10561 agcagccgac cggtgggtgt tcatcgactg ggatttcgcg ggcccgggtt cgcgtctgtg
    10621 ggacctcgcc tacaacgcgc agacctcggt cccgctccag gccgggctcg aggtttccgc
    10681 ggtcgcgccc cggctgcggg cgcttgtcga cggatacgac ctggacggca caggccgaat
    10741 cgcactcgca ggaatgctcg cccggagagc gcgggcaatg cacgagatgc tgcaacgcag
    10801 agccagcgca ggcgaagctc cgtggtcgcg gatctggatc gaagacggac cctactggga
    10861 cgaaactgcc gactatctcg ccacgaacag tgccgcctgg gccgatgcgt tgctctgagc
    10921 cgagcgatca ttccgaccga tcaccggcag ggaccaggta gtccctgtcg cgggatgcaa
    10981 cgcgcgtcaa cgcggcaata tcgggcgttc ccgaccgcgg cagtcgagcc gcttcctcgg
    11041 agtgcgatcg gcgctacgag cgggccctcg catcgtgccc cgcgagaaac tgacgctgtg
    11101 aggacgatcc gtaagtgcgt gtgagcgggt gggcgagtgt cctggtgacg ggtcacgact
    11161 cactttcgag tcgccgtttg agggtggcga cgctagtcgg catttccttg cgggcctgcc
    11221 ggcgtaccgc gagtgggacg agcagcttgc caacgccgtg gccttcgaag tcgaccgaga
    11281 tagtgacccg ggagcgcacg tgcgtgagcg gtgtcacgct gacgttgacg agcgcccgga
    11341 tcggcccatc gatgccgcgc acgctccacg cgcgtggcgg gtcggcacgg acaacttcgg
    11401 aggtgctggc acgctcggca aagccaatcc tgcgtaccgt gacgcaccgc ggcttctccg
    11461 ccgtggccgc cagctccatg tgtccgcgca caacgccctg ctgccactcg ccgaaccggg
    11521 acgggtcggt ggtgtacgcg tagacatcgt cggccgaccg tcccacatcg gcgctcacga
    11581 tgatcggcgg cattcagctg ccctcgccga acagagccga gtaccgcgct aggtacagcg
    11641 gccaaccctc gtcgttacca acgccatcgg cgacggcttc ccacccggca ccgtgcctgt
    11701 cgatgttgcg gtgctcgagt tcgacgcgcg tgcggtcggc agtctcggcg atgaatctga
    11761 cctcgacctc gctcgcattc tccggatcgg tctccagctg ccacgtcggg ccgatgtccc
    11821 agctgaacac gacccgaagc ggcggctcgt atacaagcac ccgagcccaa tggcattccg
    11881 tgccgtccgt ggcgcggtca tagatgtgac cgccgacccg cggctcaaag accgtctcgg
    11941 tgatcggcgc cgccagcaga ttgtgctctg gtggtttgaa gtcgcccagc cgctccgtga
    12001 acaccgagaa cgccttctcc gccgatacct cgacgacgat gtgcgtgcgg acgactgcgc
    12061 tgtcggcctg cgtcatgacg actcctgtct cggtcgctcg gctgatggtt gctcgacggt
    12121 gtcggtgaaa ttggcgagag cgcggctcca gaacgtgtcc agctgatccc gaagagcggc
    12181 gacggcggtc gggttgagcc ggtagatccg ccgcgttccc gccgcgcgct ccgtgaccaa
    12241 tccggcgtcc ttgagcactt tcaacgcttg ggacaccgcc gggcggctga ccggtagctc
    12301 cgccgccagt tcagtcaccg cccgcggccg ttctgccaga gccacgacaa tcgcgtgccg
    12361 agtcggatca ccgagcgccg cccacgcccc ctcagatccg taagcaacca tgaaccgtaa
    12421 gcattctcta acgcttagcc caagtcaagc gcccagccgg agaccagcaa acgcgcacgt
    12481 ccgcacatcg gcagatgatc gcgttgggca ggcggcttgt gctgccagag accccacatg
    12541 ttgccgtggt ggcgcgacct tggggccggt tgactgagct gcccggccag caggttgcgg
    12601 gatgtagcga tgtgaggatc tgttgatgaa cgcgtcatcc tcacgatcgt gtcagcgatt
    12661 ggagatggcg cgacgccaac gtgcagcgac cagaggggga ccaggcgggg accaacatgt
    12721 tgatgtgcct gctagggaac gcatctgtcc gtttggacgt
//
LOCUS       DATCBJ010000037         5167 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_362889, whole genome shotgun sequence.
ACCESSION   DATCBJ010000037 DATCBJ010000000
VERSION     DATCBJ010000037.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 5167)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5167)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5167
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_362889"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1352
                     /locus_tag="VII50_00815"
     CDS             <1..1352
                     /locus_tag="VII50_00815"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HEY5049411.1"
                     /translation="QSLVGAPNATVHLYNATAPLFRRVVFKNSRAETVALAADGTRHV
                     MKHAERLLGSTEVFGYEYSPEIFIDTELDFAVEVCEAVMDVWQPGPGREIILNLPATI
                     ERSTPNVYADQIEWMGRHLSRREFICLSLHPHNDRGCAVAASELGVMAGADRVEGCLF
                     GHGERTGNVDLVTLGMNLFSQGIDPQIDFSQVDEVRRTVEYCTQLPVHFRHPYAGDLV
                     YTAFSGSHQDAIKKGFEALAADAAAAGKSVDEIDWAVPYLPIDPKDVGRSYEAVIRVN
                     SQSGKGGIAYVMKAEHSLELPRRLQIEFSRVIQARTDASGGEVSPSEMWKIFEAEYLS
                     PSSPLTLLAHHTSAAVDQKDALSVEVRYQGSTQVLEGTGNGPIAAFLDALTTIGIDVR
                     VLDYTEHAISAGGDARAAAYLECVVGDEVLWGVGVDPNIVTASLRAVVSAVNRAADR"
     gene            1494..2225
                     /gene="recO"
                     /locus_tag="VII50_00820"
     CDS             1494..2225
                     /gene="recO"
                     /locus_tag="VII50_00820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HEY5049412.1"
                     /translation="MTLYRDDGVVLRVQKLGEADRIVTLLTRRTGRVRAVAKGVRRTK
                     SKFGGRLEPFSHVDAQFYVGRSLDIVTQVETYTAFGDRLAGDYPRYTAGTAMLETAER
                     LTAEEREPALRLYLLLVGGLRALTEETHDARLVLDAFALRALAVSGYAPALGECARCG
                     IDGPHRWFSVQGGGTVCADCRPPGSATPSSETLLLLEALLDGDWLAADASESRHQREA
                     SGLTAAYLQWHLEHSIRSLRLVEHA"
     gene            2225..3025
                     /locus_tag="VII50_00825"
     CDS             2225..3025
                     /locus_tag="VII50_00825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HEY5049413.1"
                     /translation="MAARRAVNVPAPHPSGARPPDIPAELVPRHVAIVMDGNGRWARS
                     RGLPRTSGHERGEAALLDVVEGAIEVGVGWISAYAFSTENWKRSPDEVKFLMGFNRDV
                     IRRRRDYMHSLGVRVRWAGRRPRLWASVIRELEIAEELTRDNDVLTLTMCVNYGGRAE
                     IADAARALAIDVAAGKVDPKRVDERVFARYLDEPSMPDVDLFLRSSGEQRTSNFLPWQ
                     SAYAEMVFLDTLWPDFDRRDLWRAIEIYASRDRRYGTARPNLSASGGD"
     gene            3067..4467
                     /locus_tag="VII50_00830"
     CDS             3067..4467
                     /locus_tag="VII50_00830"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase"
                     /protein_id="HEY5049414.1"
                     /translation="MASSTKKSDDRLDAIVNLSKRRGLVFPSSEIYGGLRAAWDYGPL
                     GVVLKNNVKRQWWKSMVHERDDIVGLDSSVILNPIVWETSGHITAFVDPLTECQSCHK
                     RFRADHLEEAYAEKHGKPAAHGLADINCPNCGTKGAFTEPRMFNGLLKTYLGPVEDES
                     GLAYLRPETAQGIFINYLNVQQSSRKKPPFGIAQQGKSFRNEITPGNFIFRTREFEQM
                     EMEFFVKPGSDEEWHQYWIDTRLAWYVDLGLRPDMLRLFEHPQEKLSHYSKRTVDIEY
                     RFGFQGSEWGELEGIANRTDYDLTVHSKASGVDLSYFDQETNERWTPYVIEPAAGVDR
                     CVLTFLIDAYTEDEAPNAKGELEKRTVLRLDPRLAPVKVAVLPLSRNADLSPKARDLS
                     AELRRRWNVDFDDASAIGRRYRRQDEIGTPYCVTVDFETLEDQAITIRERDTMKQERV
                     ALDQVVAYLSERLTDL"
     gene            complement(4536..4694)
                     /locus_tag="VII50_00835"
     CDS             complement(4536..4694)
                     /locus_tag="VII50_00835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5049415.1"
                     /translation="MCEFEFGNDDDPVPGAEPMSFAEYRAEWESRGSPVFRPADVADI
                     NATRERTS"
BASE COUNT          896 a         1643 c         1711 g          917 t
ORIGIN      
        1 tgcagagcct ggtcggcgcg ccgaacgcga ccgtgcacct gtacaacgcg accgcaccgc
       61 tgtttcggcg ggtggtgttc aagaacagcc gggccgaaac ggtggcgctc gccgccgatg
      121 gcacccggca cgtgatgaag cacgccgagc gactgttggg ttcgacggag gtgttcggct
      181 atgagtactc gccggagatc ttcatcgaca ccgaactcga tttcgccgtc gaggtatgcg
      241 aagccgtgat ggacgtctgg caacccggtc cggggcgaga gatcatcctg aacctgccgg
      301 cgaccatcga gcggtcgacg ccgaacgtct acgccgacca gatcgagtgg atgggccgcc
      361 acctgtcgcg gcgcgagttc atctgcctgt cgctgcatcc ccacaacgac cgaggctgcg
      421 ccgtcgccgc atcggagctg ggcgtgatgg caggtgctga ccgcgtcgag ggctgcctgt
      481 tcgggcacgg agagcgcacc ggcaacgtcg acctggtcac gctcgggatg aacctgttca
      541 gccagggcat cgacccgcag atcgatttct cgcaggtcga cgaggtccgg cgcacggtcg
      601 agtactgcac gcagctgccc gtgcattttc ggcatccgta tgccggcgac ctcgtctaca
      661 ccgcgttttc cggctcgcat caggacgcga tcaagaaggg ctttgaagcg ctggccgccg
      721 atgctgctgc cgcagggaag agcgtcgacg agatcgactg ggcggtgccc tacctgccga
      781 tcgatcccaa ggacgtcggc cgctcctacg aagcggtgat tcgcgtcaac agccagtccg
      841 gcaagggcgg catcgcgtac gtgatgaagg cggagcactc ccttgaactg ccgcgccggc
      901 tgcagatcga gttctcccga gtgattcagg cgcgcaccga tgcgtcgggc ggggaagtgt
      961 ctccgtcgga gatgtggaag atcttcgagg ccgagtacct ctcgccgtca tcgccgctca
     1021 ccttgctggc gcatcacacg tctgcggcgg tcgaccaaaa ggacgccctg tcggtcgagg
     1081 tccggtatca gggctcgacg caggtgctcg agggcaccgg caacgggccg atcgcggcct
     1141 tcctcgacgc gctgacgacg atcgggatcg acgtccgggt cctcgactac accgagcacg
     1201 ccatctccgc cggtggcgac gcgcgtgcgg ccgcctacct cgagtgcgtg gtcggtgacg
     1261 aggtgctgtg gggcgtcggc gtcgatccga acatcgtgac ggcgtcgctg cgtgcagtgg
     1321 tgagcgcggt gaaccgggcc gccgaccgct agctgatccg cgtggtagac gcctgccggt
     1381 ctcgagaccg ctcggcggct accaggctcc agctctgaga cgcgcggcgg ggacgtgatt
     1441 ggcggcagct tcagggagaa tgacccggtg actctgtatc gggacgacaa ccggtgaccc
     1501 tgtatcggga cgacggggtc gtgctgcgcg tccagaagct cggcgaggcg gaccgcatcg
     1561 tcactctgct cacccgccgg actggccgag tgcgggccgt cgcgaagggt gtgcgccgca
     1621 cgaagtccaa gttcggtggc cggctcgagc cgttctccca cgtcgatgcg cagttctacg
     1681 ttgggcgatc gctcgacatc gtgactcagg tcgagaccta cacggccttc ggcgaccggt
     1741 tggccggcga ctatccgcgc tacacggcgg ggaccgcgat gcttgagact gcggagcggc
     1801 tcacggccga agagcgcgag ccggcccttc ggctctacct cttgctcgtc ggcggcctgc
     1861 gggcgctcac cgaagagacc catgacgcgc gtcttgtgct ggacgctttc gcactgcgcg
     1921 cgcttgcggt ctccggctac gcccctgcgt tgggggagtg cgcgcgttgt ggcatcgacg
     1981 ggccgcatcg gtggttctcg gtgcagggcg gcggcacggt gtgcgccgac tgccgtcccc
     2041 ctggctcagc taccccgtcg tccgagacgc tgctgcttct cgaagctttg ctggacggcg
     2101 attggctggc cgccgatgcc agcgaatcgc ggcatcaacg tgaagcgagt gggttgaccg
     2161 ccgcctatct gcaatggcat ctcgagcaca gcatccgttc gctacggctc gtcgagcacg
     2221 cctgatggct gcgcgccgcg cggtcaacgt cccggcaccg catccctcgg gcgcccggcc
     2281 tcctgatatt ccggccgaac tggtgcccag gcacgtcgcc atcgtgatgg acgggaatgg
     2341 tcgatgggcg agatctcgcg gccttccccg tacatctggc cacgaacgcg gggaggcggc
     2401 gctgctggac gtcgtcgagg gcgccatcga ggtcggcgtc ggctggatct cggcctacgc
     2461 attttccacc gagaactgga agcgctcacc cgacgaagtg aagttcctga tgggcttcaa
     2521 tcgcgacgtg attcgccgcc gccgcgacta catgcactca ctcggcgtgc gagttcgctg
     2581 ggccggacgc cgtccgcgat tgtgggcgag tgtgattcgc gaactcgaga tcgccgagga
     2641 attgacccgc gacaacgacg tcctcacgct gacgatgtgc gtcaactacg gcggtcgcgc
     2701 cgagatcgcc gatgccgcgc gagcattggc gattgacgtg gccgcaggca aggtcgaccc
     2761 caaacgcgtg gacgagcggg tcttcgcgcg ctacctcgac gagccttcga tgccggacgt
     2821 cgatctgttc ctccgttcgt caggtgaaca acgaacgtcg aacttcctgc cctggcagtc
     2881 ggcgtacgcc gagatggttt tcctcgacac gctgtggccg gacttcgacc ggcgcgatct
     2941 gtggcgggcg atcgagatct acgccagccg cgatcggcgg tacgggacgg cgcgaccgaa
     3001 cctctcggcg tccggcgggg attgatacgc tgagcgctca ccgaccgcca gccggatgga
     3061 catttcatgg ccagctccac caagaagtcc gacgaccgac tcgacgccat cgtcaacctg
     3121 agcaagcgcc gcgggttggt cttcccgagt agcgagatct acggcggcct gcgggctgcg
     3181 tgggactacg gaccgcttgg cgtcgtgctc aagaacaacg tgaagcgcca gtggtggaag
     3241 tcgatggtgc acgagcgtga cgacatcgtc gggctcgact cgtcggtcat tctcaatccg
     3301 atcgtgtggg agaccagcgg tcacatcacc gcgttcgtcg acccgctgac ggagtgccag
     3361 tcgtgccaca agcgttttcg cgcggaccat ctcgaagagg cgtatgccga gaagcacggc
     3421 aagccggcag cgcacgggtt ggcggacatc aactgtccca actgcgggac caagggtgcg
     3481 ttcaccgagc cacggatgtt caacgggttg ctgaagacct acctcggtcc ggtcgaggac
     3541 gaatcgggcc tggcctatct gcggcctgag acggcgcagg gcattttcat caattacctc
     3601 aacgtgcagc agtcgtcgcg caagaagccg ccgttcggta ttgcgcaaca gggcaagagc
     3661 tttcgcaacg agatcacgcc gggaaacttc atcttccgga cgcgcgagtt cgagcagatg
     3721 gagatggagt tcttcgtcaa gccgggtagc gacgaggaat ggcaccaata ctggatcgac
     3781 acccggctgg cgtggtacgt cgacctcggt cttcgtccag acatgctgcg gctctttgag
     3841 caccctcaag agaagttgtc gcactattcc aagcgcacgg tcgacatcga gtatcgcttc
     3901 ggattccaag gctcggagtg gggcgagctg gaaggaatcg cgaaccgcac cgactacgac
     3961 ctcaccgtgc actcgaaggc ttcgggcgtc gacttgtcgt acttcgacca agagaccaac
     4021 gagcgctgga cgccgtacgt gatcgaaccg gcagctggtg tcgaccggtg cgtgctgacg
     4081 tttctcatcg acgcttacac cgaggacgaa gcgccgaacg caaaaggcga actggagaag
     4141 cgcaccgtgc tgcggctcga tccgcggctc gcaccggtca aggtcgccgt gctgccgctg
     4201 tcgcgcaacg ccgacctgtc accgaaggcg cgcgatctgt cggcggagct gcgtcgtcgc
     4261 tggaacgtcg acttcgatga tgcatcggcg atcggacgtc gttaccgccg tcaagacgag
     4321 atcgggacgc cgtactgcgt gaccgttgac tttgagacgc tggaagatca agcgatcaca
     4381 attcgtgaac gcgacacgat gaagcaagaa cgagtcgcgt tggatcaggt ggtcgcctac
     4441 ctaagcgagc gtctgaccga cctctagcgc ggggctttcc ctgacccgtg aatcctgggt
     4501 gatcgctgtc gctcccgcga cggtgggctc acgatttagc tggtgcgttc gcgggtggcg
     4561 ttgatatccg cgacgtctgc tgggcggaat accgggctgc ccctcgattc ccattcggct
     4621 cggtactcgg cgaacgacat cggctcggcg cctggcacgg gatcgtcatc attgccgaat
     4681 tcgaattcgc acatcgggca gacctcgtag gtcgcctccc cgagccagtc cctgtagggc
     4741 ggccgattgc tagtcctccg ggcggtggcc agatggcgta gggcttgcgg cggagcctta
     4801 cgaactggca caccgggcac tcagacatgt cattacttcc tttggcatgt gagctcggtt
     4861 acgaaatcga acgttccgtc atctctgggc cagagatgac gggcccaatc taaagcgccc
     4921 ggatccgtca gctttctcga acttccccag gatgtcgcgc atgccatcgc ttcgagatga
     4981 ggtgatgcgg cgccggtcca cctagggtct cttccaacga cgtccgcgag ggcggcgcac
     5041 cgctcgccaa cggacacact cgacattccc ggcggcacat caggaagcgg cgccatcgcc
     5101 ggacggacct gcatcagtac ggtcacgacc acgacgtcgc ccgacgcgtc cttaaggata
     5161 cgcgatg
//
LOCUS       DATCBJ010000038         4579 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_362848, whole genome shotgun sequence.
ACCESSION   DATCBJ010000038 DATCBJ010000000
VERSION     DATCBJ010000038.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 4579)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4579)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4579
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_362848"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..348
                     /locus_tag="VII50_00840"
     CDS             <1..348
                     /locus_tag="VII50_00840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016461675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HEY5049416.1"
                     /translation="DQAAIEGLPPGAGLLVVRRGPNAGSRFLLDADVTTAGRHPESDI
                     FLDDVTVSRRHAEFRKTPDGFFVRDVGSLNGSYVNRERIDDSALQGGDEVQIGKFRLV
                     FLTGHRPGDPAAQ"
     gene            360..1100
                     /locus_tag="VII50_00845"
     CDS             360..1100
                     /locus_tag="VII50_00845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEY5049417.1"
                     /translation="MSARSTSRASLSIGEVLTQLRGDFPDVTISKIRFLESEGLVEPD
                     RTSAGYRKFSHADVHRLRYVLTAQRDHYLPLRVIKDHLDALDRGLEPADHANAGPRVP
                     MALIATDGLPGPEAFGTDPAEVRLSRKELMDAAELDEAALGQLEQYGLVAPRPGSNHY
                     DGTSLVVARTVAEMSRFGIEPRHLRSFKAAADREVGLVEQVVTPLVRQRNPEARARAE
                     EVARELAALSVRLHASLVKAGLRPGLSR"
     gene            1238..1717
                     /locus_tag="VII50_00850"
     CDS             1238..1717
                     /locus_tag="VII50_00850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018386697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nuclease family protein"
                     /protein_id="HEY5049418.1"
                     /translation="MNELHVVGVRVELPTNQPIVLLREVEGERYLPIWIGAVEATAIA
                     FAQQGVVPPRPLTHDLMRDVLAALGAKLRAVRITEMRDNVFYAELVFDDGREVSARPS
                     DAIALALRTGASIFGSESVLEEAGIEIADEHEAEDEVEKFREFLDTISPEDFDTTQG"
     gene            1952..2539
                     /locus_tag="VII50_00855"
     CDS             1952..2539
                     /locus_tag="VII50_00855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019311013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEY5049419.1"
                     /translation="MTPAPLTPVQPGNSSRQGSLFTDDFPVPDDGVGYRGPVACSAAG
                     ITYRQLDYWARTGLVEPTVRAAAGSGSQRLYGFRDILVLKVVKRLLDTGVSLQNIRAA
                     VQHLRERGIDDLAQITLMSDGASVYECTSADEVIDLVQGGQGVFGIAVGRVVREVEGS
                     LAVLPSERPETDDAAPTDASAAEDELARRRARRIG"
     regulatory      2582..2676
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            2799..>4579
                     /locus_tag="VII50_00860"
     CDS             2799..>4579
                     /locus_tag="VII50_00860"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009074223.1"
                     /note="acts in conjunction with GvcH to form
                     H-protein-S-aminomethyldihydrolipoyllysine from glycine;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine dehydrogenase
                     (aminomethyl-transferring)"
                     /protein_id="HEY5049420.1"
                     /translation="MNSSRPTPVSPGRAPRSLRSLESSRTFADRHIGPDADEQAKMLA
                     VVGYGFLEELAAQAIPASIADVPDRQSSIPPAATEEEVTEELRALAAQNTVLTPMIGL
                     GYYGTLTPAVIRRNVLENPAWYTAYTPYQPEISQGRLEALLNFQTVISDLTGLPVAGA
                     SLLDEPTAAAEAMALCRRNSSSSSNRFYVDADVLPQTIAVIHTRALPMGLDVVVVDLA
                     NGLPADGCFGVLIQDPGTSGRVRDHAALAAQAHSAGALVVAATDLLASTLLRPPGEWG
                     ADIAVGSSQRFGVPLWFGGPHAGFIAVREALVRSLPGRLVGQSIDADGQPAYRLALQT
                     REQHIRREKATSNICTAQVLLAVTAAMYAVYHGPDGLTDIAARVHRNAAVLAEGLRRG
                     GIDVATGAAEFFDTVQVSVPGRAAAIVAAACERGINIRLIDADTLSMACDETTQRQHL
                     ASLWAAFGVGLSVDDIDSIDDTVRDEFASPVRRTSDFLRHQVFHQHRSETSMLRYLRS
                     LSDKDVALDRSMIPLGSCTMKLNATTEMEPISWPEFAQIHPFAPIEQATGYLTLIRQL
                     ESWLADVTGYDAVSLQPNAGSQGELAGL"
BASE COUNT          755 a         1507 c         1547 g          770 t
ORIGIN      
        1 gaccaggcgg ccatcgaagg gctgccgccg ggcgccggac tcctcgtggt ccgccggggt
       61 ccgaacgccg gcagcaggtt cctgctggat gccgatgtca ccaccgcggg tcgccatcca
      121 gagagcgaca tctttctcga cgacgtcacc gtctcgcggc gccacgcgga gttccgaaag
      181 acgccggacg gcttcttcgt gcgtgacgtc ggcagcctga atgggagcta cgtcaaccgc
      241 gagcgcatcg atgactccgc actgcagggc ggcgatgagg tgcagatcgg aaagttccgg
      301 ctggtcttct tgaccgggca ccggcccggc gatcccgcgg cacagtgacg gcgccagcga
      361 tgagcgcccg atcgacctct cgcgcgtcac tgtctatcgg tgaggtgctg acgcagttgc
      421 ggggagactt tcccgatgtc acgatttcga agatccggtt cctcgagtcc gagggcttgg
      481 tcgaaccaga ccggacgtct gccggctacc gaaagttctc ccacgctgac gtgcaccggc
      541 tgcgttacgt gctcacggct caacgcgacc actacctgcc tctgcgggtg atcaaggacc
      601 acctcgacgc actcgaccgt ggtctcgagc cagccgatca cgccaacgcc ggtcctcggg
      661 ttccgatggc gctgatcgcc accgacggtt tgcccggtcc tgaagcattc ggcaccgatc
      721 cggcggaagt ccggctgtcg cgcaaggagc tgatggacgc ggccgagctc gatgaggcag
      781 cgcttggcca gctcgagcag tacggtctgg tcgcgccccg ccccggcagc aaccactacg
      841 acggaacgtc gttggtcgtc gcgcgcacgg tggcggagat gtcgcgcttc ggcattgagc
      901 cgcgtcactt gcggtcgttc aaggcagcgg ccgaccgcga ggttggcttg gtcgagcagg
      961 tcgtgacgcc gctcgtacgc cagcgtaatc cggaggccag ggcgcgtgct gaggaggtag
     1021 cccgcgaact cgcagcactc tcggtgcgcc tgcacgcgtc cttggtgaag gccgggttgc
     1081 gtcccggtct gagccggtag cgcttgctgc gcccacccac ccgaatcgtc catggattga
     1141 cgatcggatg agctcggctg ttggcccgac ttgcggtgta gcgtgagccg caggcggcag
     1201 gcgactggcg gaaactagcg agggcagggc ggtcaccgtg aacgaactgc atgtggtggg
     1261 cgttcgggtc gaactgccaa cgaaccagcc gatcgtgctg ctgcgcgagg tcgaaggcga
     1321 gcgctacttg ccgatctgga tcggcgctgt cgaggcgacc gccattgcct tcgcgcaaca
     1381 aggcgtcgtg cccccgcgtc cgctgaccca cgatttgatg cgcgacgtgc ttgccgcact
     1441 tggcgcgaag cttcgcgccg tccgtattac cgagatgcgc gacaacgtct tctacgccga
     1501 actggtcttc gatgacggtc gcgaggtcag tgcgcggcca agtgacgcga tcgcattggc
     1561 cctgcgtacc ggagcgtcaa tcttcgggtc cgagtccgtg ctcgaggaag ccggcatcga
     1621 gattgccgat gagcacgagg ccgaggacga agtggagaag ttccgcgagt tcctcgacac
     1681 catttcgccc gaggatttcg ataccaccca gggttagtgc cgcgacggcg acacttctag
     1741 gcgtggctct gaccctcgac cagaggttca gggttgcggg tttgcagtag gttctcgcgc
     1801 ttccgcgcgt cgcgttgact cgggcggggt gcgtccatag cgtggcagtt ccggccgtgg
     1861 tacgaggcag tggcatgaga gaggacgtca ggtggcgaac aacagtgagc ccccagttgc
     1921 agggtggacg tcgccgctga caccggcgcc gctgacacca gcgccgctga caccagtgca
     1981 accaggtaac tccagccggc agggctcgtt gttcaccgac gacttcccgg tgcccgacga
     2041 cggcgtcggc taccgcgggc cggtcgcctg ctcggcggcc ggcatcactt accggcaact
     2101 cgactactgg gcgcgcaccg gattggtcga accgaccgtt cgcgctgccg ccggatcggg
     2161 ctcgcaacgc ctgtacggat tccgcgacat cctggtcttg aaggtcgtca agcggctcct
     2221 cgacacgggc gtgtcgctgc agaacatccg ggcggccgtg cagcacttgc gcgagcgcgg
     2281 gatcgacgat ctcgcccaga tcaccctgat gagcgacggc gcaagcgtct atgagtgcac
     2341 ctccgccgac gaggtcatcg acctcgtgca gggcggtcaa ggcgtgttcg ggatcgcggt
     2401 cgggagagtc gtccgcgagg tcgaggggtc cctggcggtc ctgcccagcg agcggcccga
     2461 gaccgacgac gcggcaccga cggacgcttc ggcggcggag gacgaacttg cccgccgtcg
     2521 ggcgcgccga atcggctgag cccccacatc ggtcgcgcct gcagtaacgt ggagccgccg
     2581 acgacccctc gcgggagagt tctcacgctg ccagcgtgag gcgccgaagg agcaacctcc
     2641 ccggaatctc tcaggcccag cgaccgtgcg ggcgaggcga ctctggaaag aacgtgcctg
     2701 atggcgcgtc accgaaggtg aaagccggcg aacgcgtcgg cgaagctctc aggtgcccac
     2761 gacagagggg gagtccgatc cggcgcccgg aggctccctt gaactcgtcc cgtcccaccc
     2821 cggtgtctcc cggtcgcgcg ccacggtcgc tgcgcagcct cgaatccagt cgcacgttcg
     2881 ccgacagaca catcgggccg gacgccgacg agcaggccaa gatgctggca gtcgtcggct
     2941 acggctttct cgaagagctt gcggcgcagg cgatcccagc atcgatcgcc gacgtgcccg
     3001 atcggcaatc ctcgatcccg cctgctgcga ccgaggagga ggtcaccgaa gagctgcgcg
     3061 cgctggctgc ccaaaacacg gtcctgactc ccatgatcgg gctcggttac tacgggacgc
     3121 ttacgccagc ggtcatccgc cgaaacgtcc tggagaaccc cgcctggtac acggcgtaca
     3181 cgccgtacca gcccgagatc tcgcagggca ggctcgaggc actgctcaac ttccagacgg
     3241 tgatcagcga cctgaccgga cttccggtcg cgggcgcttc gttgctcgac gagccgacag
     3301 ctgccgcgga ggcgatggcg ctgtgtcggc gcaactcctc gtcgtccagc aatcgtttct
     3361 atgtcgacgc cgacgtgttg ccgcagacga tcgccgtgat acatacgcgc gcgctaccca
     3421 tgggcttgga cgtcgtcgtc gtcgatctcg cgaacggcct cccggccgac ggctgcttcg
     3481 gtgtattgat ccaggaccct ggcacgagtg gccgggtgcg agatcatgct gcacttgctg
     3541 cgcaggcgca ctcagccggc gcactcgtcg tggctgcgac ggatctgctg gcatcgacgt
     3601 tgctgcggcc tccgggtgag tggggcgcgg acatcgccgt gggatccagc cagcggttcg
     3661 gggttccctt gtggttcggt ggaccgcacg cgggattcat cgccgtccgc gaggcattgg
     3721 tgcggtcgtt gcccggacgg ctcgtcggcc agtcgatcga cgccgatggg cagcccgcgt
     3781 accggctcgc gttgcagacc cgcgagcaac acatcaggcg cgaaaaggcg acgagcaaca
     3841 tctgcactgc acaggtgctg ctcgcagtca cagcagcgat gtatgcggtc taccacgggc
     3901 ccgacggctt gaccgacatc gccgcgcgcg tgcaccgcaa cgcggcagtt ctcgcagagg
     3961 gcctgcggcg tggtggtatc gacgtggcga ccggggcggc ggagttcttc gacaccgtgc
     4021 aggtgagcgt tccaggtcgc gcggccgcga tcgtggccgc tgcgtgcgag cgcggtatca
     4081 acattcggtt gatcgatgcg gacacgctga gcatggcgtg cgacgagacg acgcagcggc
     4141 agcacctcgc gtcgttgtgg gccgcgttcg gagtcggcct gagtgtcgac gacatcgact
     4201 cgatcgatga caccgtgcga gacgagttcg ccagcccggt tcgaaggacc tcagatttcc
     4261 ttcgccacca ggtgtttcac cagcaccggt ctgaaacgtc gatgctgcgc tacttgcgca
     4321 gcctttccga caaggacgtt gctctcgacc gatcgatgat cccgctcggc tcgtgcacga
     4381 tgaagttgaa cgcgactacg gagatggagc ccatcagctg gccggagttc gcgcagatcc
     4441 atcccttcgc gccgatcgag caggcaacgg gctacctcac cctcatccgg caactggagt
     4501 cgtggctcgc cgacgtcacg ggatatgacg cggtctcgct tcagcccaac gccgggtcac
     4561 aaggcgagct cgcgggcct
//
LOCUS       DATCBJ010000039         3333 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_68709, whole genome shotgun sequence.
ACCESSION   DATCBJ010000039 DATCBJ010000000
VERSION     DATCBJ010000039.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 3333)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3333)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3333
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_68709"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..927
                     /locus_tag="VII50_00865"
     CDS             <1..927
                     /locus_tag="VII50_00865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009079167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEY5049421.1"
                     /translation="DPRTAVTGGVTIARIFEEAGLPPGVLQMLPGGADVGEALVTHPL
                     VGVISFTGSTAAGRRVAELAGKHLKRVHLELGGNSALIVLDDADVDAAVNLAAWGSFF
                     HQGEICMTTGRHLVHTRLHDEFVDKLAAKALHLPVGDPATGHVALGPLIDAGQRDKVH
                     HLVTSSVAAGAQVAAGGSYEDLFYKPTVLSGVSLTSPAFAQEVFGPVAPVIRFADAEE
                     AVAIATSTEYGLSLGIVTRDVMKGLDLAAQIPTGIVHINDQTVNDEANSPFGGVGASG
                     TGSRFGGAAANVDAFTETQWVTIRSEPPTYPF"
     gene            1120..1248
                     /locus_tag="VII50_00870"
     CDS             1120..1248
                     /locus_tag="VII50_00870"
                     /inference="COORDINATES: protein motif:HMM:NF019845.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HEY5049422.1"
                     /translation="MLGHEGAGTVETVGSGVTEFAPGDRVVIGWPSCGTLPQLSGG"
     gene            1589..1882
                     /locus_tag="VII50_00875"
     CDS             1589..1882
                     /locus_tag="VII50_00875"
                     /inference="COORDINATES: protein motif:HMM:NF012335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HEY5049423.1"
                     /translation="MRHDSRPTLASERGATHVVNVTTDEPLAAIASICGGPADYALEC
                     TGVIDIVRQAADCVGMLGTCILIGGAPAAAEFSLDHRSTSWGKRIVGAPGERS"
     gene            2065..2661
                     /locus_tag="VII50_00880"
     CDS             2065..2661
                     /locus_tag="VII50_00880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020662573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphatase"
                     /protein_id="HEY5049424.1"
                     /translation="MPSRQLVLASASPARLRLLWDAGFDPIVMVSGVDESAVENSDPA
                     DLVEILAVRKAAAVAKHVPASSVVIGCDSMLSFDGDVMGKPPTPEAARHYWQRMRGRE
                     GVLVTGHCVVDVGSGREASAVASTIVHFGTPTDAELDAYINTGEPMKVAGAFTLAGFA
                     GPFVDGIEGDHGNVIGLSLPLLRRLLADIEIRIVDLWR"
     gene            2652..>3333
                     /locus_tag="VII50_00885"
     CDS             2652..>3333
                     /locus_tag="VII50_00885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006549575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HEY5049425.1"
                     /translation="MALKIGPIEVDPPIVLAPMAGVTNVPFRQLCRSYGAALYVSEMV
                     SARAVIEHNPNTDAMVRFGADEPVRSLQLYGVDPDVMYAAVRYAVGEIGVDHIDLNFG
                     CPAQKVNRLGGGAALPLRRVLFRAVVAAAVKAAGTVPVTVKMRKGIDDATLTYLDAAR
                     AAEDVGVAAIALHARTAEQLYSGAADWSAITELREAITSVPVLGNGDIWAADDALRMM
                     RETGCAGVV"
BASE COUNT          508 a         1055 c         1143 g          627 t
ORIGIN      
        1 gatccgcgca ccgcggtgac cggcggcgtc acgatcgcca ggatcttcga ggaagcgggg
       61 ctgccacccg gagtccttca gatgctgccc ggtggcgctg acgtcgggga agcgttggtg
      121 acgcatccct tggtcggcgt catctcgttc acgggttcga cggcggccgg acgtcgggtc
      181 gccgaactcg ccggcaagca tctgaagaga gtgcatctgg aactgggtgg taactccgcg
      241 ctcatcgtcc ttgacgacgc cgacgttgac gcggcggtca acttggcggc gtggggatcg
      301 ttcttccatc agggtgagat ctgcatgaca accggacgtc acctggtgca cacgcggctg
      361 cacgacgagt tcgtcgacaa gctggccgcg aaggccctcc acttgccggt cggtgatccc
      421 gctaccggcc acgttgcgct cgggcccctc atcgatgctg ggcagcgtga caaggtgcat
      481 cacctcgtaa cgtcgagcgt cgcggccggc gcccaggtcg ccgctggtgg cagttacgaa
      541 gacttgttct acaagccgac cgttctcagc ggcgtctcgc ttacgtcgcc tgctttcgcg
      601 caggaagtct tcgggccggt cgcgcccgtc atccgcttcg cggatgccga ggaggctgtg
      661 gccattgcga cgtcgacaga gtacggattg tctctcggca tcgtgacgcg tgacgtcatg
      721 aagggcctcg acctcgccgc gcaaatcccg accggcatcg tgcacatcaa tgaccagacc
      781 gtcaacgatg aagcgaacag tccgttcggt ggcgttggcg cttccgggac cggttcgcga
      841 ttcggcggtg ccgcggccaa cgtcgatgcg ttcaccgaga cccagtgggt gacgatccgc
      901 agcgagccgc cgacctatcc gttctgagcg aaaggttgca cctgtggcga ccgagatcac
      961 ggcggcggtt gtcgaggcgc aggtcaagcc attcgagttg cagactctcg agttcgatga
     1021 gccgggcgcg ggcgaggtgc tcgttcgcct cgtcgctgcc ggcgtttgcc acaccgacgg
     1081 actcgcccgc catggcgact tgcctttccg tttcctggcg tgctcggcca cgagggcgcc
     1141 ggcaccgtcg agacggtcgg ctcgggggtc acggagtttg ccccgggaga ccgcgtggtc
     1201 atcggctggc cgtcatgtgg aaccctgccg caactgtcag gcgggtgagc cccgctattg
     1261 ccaacacctc ggcgaactgt tggcgggcgg cactcgacgt ggtggtacct ccgcctttgc
     1321 atcggtcgga cgggtcggcg gtttcgagtc acttcttcgg gcagtcgtgg ttcgccacct
     1381 atgcctggac gacaccacgc tcgctggtcg ccgtcccggc ctgatgttcc gttggagatg
     1441 atgggcccgc tcgcgtgcgg cgtgtcgacc ggcgcgggcg cggtcttcaa cactgctcga
     1501 ccgacgcggg atcgagcatc gcggtgttcg gcgtcagcac cctagggctt gccgcgatca
     1561 tggccgcacg caactcgcct gcgacgaaat tcgccatgat tcacgtccca cgctcgcgtc
     1621 cgaacgcggc gcgacacacg tcgtcaacgt cacgaccgac gagcccctcg cggcgatcgc
     1681 gtcgatctgt ggtgggccgg ccgactacgc attggaatgc accggcgtca tcgacatcgt
     1741 cagacaagcg gccgactgcg tcggcatgct cggcacctgc atcctcatcg gaggtgcgcc
     1801 ggccgcggcg gaattcagcc tcgaccaccg gtcgacctcg tggggcaagc gcatcgttgg
     1861 cgcgcccggc gaacggtcgt agcgcagaac tcatcggtgg cttgatcgac ctccacgcgt
     1921 tgggcaggtt cccctcgatc gccttgtctc gtacttcgac ctcgacgagg tcgagactgc
     1981 attggcgagg tcgtacgacg gatccgtcgt caagccgtcc tgcgaatgcc tacctgacgc
     2041 tggttgcctt aagctcgtgg tctgatgccg tcccgccagc ttgtcctcgc ttcggcgtcc
     2101 ccggctcggc tgcgcctcct atgggacgcc gggtttgatc cgatcgtgat ggtcagcggc
     2161 gtcgacgaga gcgcggtcga aaacagcgat ccggccgatc tcgtcgagat actcgcggtg
     2221 cgcaaggcgg ccgcggtcgc caagcatgtg ccggcgagtt cggtggtgat cgggtgcgac
     2281 tcgatgttga gcttcgacgg tgacgtgatg ggtaagccgc cgacgccgga ggccgcgcgg
     2341 cattattggc agcgcatgcg cggccgtgag ggcgtgcttg tcactggtca ttgcgtcgtc
     2401 gacgtcgggt cgggtcgcga ggcatcggcg gtggcaagca ccatcgtgca tttcgggacg
     2461 ccgacagatg ccgaactcga tgcctacatc aacaccggcg aaccgatgaa ggttgctgga
     2521 gcgttcacac tcgccgggtt cgccgggccc ttcgtcgatg ggatcgaagg cgatcacggc
     2581 aacgtgatcg gcctctcgtt gccgttgctg cgccggcttc tcgccgacat cgagatcagg
     2641 atcgtggatc tgtggcgctg aagatcgggc cgatcgaggt cgatccgccg atcgtgcttg
     2701 cgccgatggc gggcgtgacc aacgtgccgt ttcgccagct gtgccgaagc tacggcgcgg
     2761 cgctttatgt gagcgagatg gtgtctgcgc gtgcggtcat cgagcacaat cccaataccg
     2821 atgcgatggt gcggttcggt gccgacgagc cggttcgcag cctgcagctc tacggcgtcg
     2881 accccgacgt gatgtacgca gctgtgcggt acgccgtcgg cgagatcggc gtcgaccaca
     2941 tcgatctcaa cttcgggtgt ccggcgcaga aggtcaaccg gctcggcggc ggtgccgcgc
     3001 tgccgcttcg ccgcgtgctg tttcgtgctg ttgtggccgc cgcagtgaag gcggcgggca
     3061 ccgttccggt gacggtaaag atgcgcaaag gcatcgacga cgcaacgctg acttacctcg
     3121 acgctgcacg cgcggccgaa gatgtcggtg tagccgcgat cgcgttgcac gcgcgaaccg
     3181 ctgagcagct gtattcgggc gcggccgact ggtcggcgat cactgagctg cgagaagcga
     3241 tcacgtcggt gccggtcttg ggcaacggtg acatctgggc cgccgacgat gcgctgcgaa
     3301 tgatgcgcga gacgggatgt gcgggtgtcg tgg
//
LOCUS       DATCBJ010000040         2537 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_546250, whole genome shotgun sequence.
ACCESSION   DATCBJ010000040 DATCBJ010000000
VERSION     DATCBJ010000040.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2537)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2537)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2537
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_546250"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            283..>2537
                     /locus_tag="VII50_00890"
     CDS             283..>2537
                     /locus_tag="VII50_00890"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-translocating pyrophosphatase"
                     /protein_id="HEY5049426.1"
                     /translation="MLGLDLVSATNGSATSVTGGNYTWVWIVAVVALLALGVAFALVR
                     EVLSADQGTENMKRIAGAVQEGASAYLKRQFRTLGIFAVIVFFLLLLLPADSGTQRIG
                     RSLFFLVGAGFSGVTGYTGMWLAVRANVRVAAAARGGESRGAMRIAFRTGGVAGMFTV
                     GLGLFGACLVVLFFRANAPKVLEGFGFGAALLAMFMRVGGGIFTKAADVGADLVGKVE
                     QGIPEDDPRNAATIADNVGDNVGDCAGMAADLFESYAVTLVASLILGKTAFGNLGLVF
                     PLIVPAIGVLTAVVGIFAVSPRSGDRSGMTAINRGFFISAGFSVVLVAVLAFTELPAK
                     FSQLKGLTNSDIASHSGNPRVFALIAVVIGIALAAIIQQLTGYFTETNRRPVRDIGRT
                     SLTGPATVILAGVSVGLESAVYAALIVAAAVYGVFLLGGGITVLSLFAVALAGTGLLT
                     TVGVIVAMDTFGPVSDNAQGIAEMSGDVTGQGAVVLTQLDAVGNTTKAITKGIAIATA
                     VLAATALFGSFTSAISDALTTAGTSASGLTGITAASLSTDVTSPNVIVGAIIGASVVF
                     FFAGLLISAVARAAGAVVYEVRRQFREIVGIMEGTALPEYGKVVDIVTRDSLRELATP
                     GLMAVLAPIATGFALGVGALAGYLVGAIAAGALMAVFLANSGGAWDNAKKLVEDGNHG
                     GKGSPAHEATIIGDTVGDPFKDTAGPAINPLLKVMNLVSLLIAPAVVTWSIGKDQNTP
                     LRVIIAIVAAA"
BASE COUNT          368 a          816 c          871 g          482 t
ORIGIN      
        1 gggctggccg tcatcagcgg actcgtcgat gacgtgacgg tcgagtacct cgcggccgga
       61 tccgtggtca cgatgacctg gccgatcggg taaccgccag atgctccccg ctcgcagatg
      121 tcagctgagg tgggacgtga cttctgacac acgcactacc agggcaatag tgggagacat
      181 agactcccgc aaccgctttg acgtccgact agttggccgg gcagcgcggg gtccacaggt
      241 ttctcgcttc tgcaatggcg cagtggcggt cgaggaggac acatgcttgg gctagacctg
      301 gtcagcgcaa cgaatgggtc tgcgaccagc gtcacgggcg gcaactacac atgggtgtgg
      361 atcgtcgcgg tcgtggcgtt gctcgcgctc ggagtggcct tcgcgctggt gcgcgaggtg
      421 ctgtcagccg accagggcac cgagaacatg aagcggatcg cgggcgctgt tcaagagggc
      481 gccagtgctt acctcaagcg gcagtttcga acgcttggca tcttcgccgt catcgtgttc
      541 ttccttctcc tgttgctgcc tgccgactcg ggcacccagc gcatcggccg gtcgctcttc
      601 ttcttggtcg gtgccggctt ctccggcgtg accggctaca ccggcatgtg gctcgcggta
      661 cgcgccaacg ttcgggtcgc cgcggccgca cgcggtggtg agtctcgcgg tgcgatgcgt
      721 attgcgttcc gcacgggcgg cgtcgccggc atgttcaccg tcgggctcgg cttgttcggc
      781 gcctgcctcg tcgtgctgtt tttccgcgcg aacgcaccca aggtgcttga gggctttggt
      841 ttcggcgcgg ctctgctcgc gatgtttatg cgtgtcggcg gcggcatctt caccaaggcc
      901 gccgacgtcg gagctgacct ggtcggaaag gtcgagcaag ggatccccga agacgacccg
      961 cgcaacgcgg cgacgatcgc cgacaatgtc ggcgacaacg tgggcgactg cgcaggtatg
     1021 gccgcggacc tgttcgagag ctacgccgtc acgctcgtcg cgtcgctgat tctcggcaag
     1081 acagcgttcg gcaacctcgg gctcgtcttc ccactcatcg tgccggcgat cggcgttctc
     1141 actgcggtcg tcggcatctt cgccgtctca ccgcgctccg gtgaccgcag tggcatgacg
     1201 gcgatcaacc gaggcttctt catctcggca gggttctccg tcgtgctcgt tgcggttctt
     1261 gccttcaccg agctgccggc gaagttctct cagctgaagg gcctgaccaa cagcgacatc
     1321 gcgtcgcact cggggaaccc gcgggtgttc gcgctgatcg cggtggtcat cgggatcgcc
     1381 ctcgccgcga tcatccagca gttgaccggg tatttcactg agacgaaccg gcgcccggtg
     1441 cgagacatcg gcaggacctc actgacgggg ccggcgacgg tgatcctcgc cggggtatcg
     1501 gtggggctcg agtctgcggt gtacgcagcg ttgatcgtcg ccgccgcggt ctacggcgtg
     1561 ttcttgcttg gcggcggcat caccgtgctg tcgctattcg ccgtcgcgtt ggcgggcacc
     1621 ggcctgctga cgaccgtcgg cgtcattgtc gcgatggaca ccttcggacc cgtgtccgac
     1681 aacgcccaag gcatcgccga gatgtcgggc gatgtgaccg ggcagggcgc cgtggtcctg
     1741 acccagctcg acgcggtcgg caacaccacc aaggccatca ccaagggcat cgcgatcgct
     1801 accgccgtgt tggccgctac cgcgctgttc ggttcgttca cgtcggcgat cagcgacgct
     1861 ctcacgaccg ccggcacctc ggcgtcgggc ctgacaggta tcacggcggc gagcttgtcg
     1921 accgacgtca cctcaccgaa cgtcattgtc ggcgcgatca tcggcgcatc ggtcgtgttc
     1981 ttcttcgcgg gcttgctcat cagcgccgtg gcgcgggcgg ccggcgctgt ggtctatgag
     2041 gtgcggcggc agttccgcga gatcgtcggg atcatggagg ggacggcgct gccggagtac
     2101 ggcaaggtcg tcgacatcgt tacccgcgac tcgctacgtg aactcgcgac acccgggctg
     2161 atggcggtgc tcgcgcccat cgcgacgggc ttcgcgctcg gagtcggcgc gctcgcgggg
     2221 tacctcgtgg gcgcgattgc cgcgggcgcg ctgatggcgg tgttcctcgc gaactccggt
     2281 ggagcgtggg acaacgccaa gaagctggtc gaggacggta accacggcgg caagggttcg
     2341 cccgcgcacg aggcgaccat catcggtgac acggtcggtg acccgttcaa ggacaccgcc
     2401 ggcccggcga tcaacccgct gctcaaggtg atgaacctgg tgtcgctgct gatcgctccg
     2461 gccgtcgtga cctggagcat cggcaaggac cagaacaccc cgctgcgggt gatcatcgcg
     2521 atcgtggcgg cggcgat
//
LOCUS       DATCBJ010000041         3455 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_510240, whole genome shotgun sequence.
ACCESSION   DATCBJ010000041 DATCBJ010000000
VERSION     DATCBJ010000041.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 3455)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3455)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3455
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_510240"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            443..772
                     /locus_tag="VII50_00895"
     CDS             443..772
                     /locus_tag="VII50_00895"
                     /inference="COORDINATES: protein motif:HMM:NF013677.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEY5049427.1"
                     /translation="MLESLGREDQQMPRSYPPEFRRQVIELARAGTRVKQLAVTFEMS
                     DATIYSWLKQDRIDRGEAEGATTSQQLELAAAKRRIRQLETELAVARKVSEVFLSEGI
                     SPKGSTR"
     gene            829..1047
                     /locus_tag="VII50_00900"
                     /pseudo
     CDS             829..1047
                     /locus_tag="VII50_00900"
                     /inference="COORDINATES: protein motif:HMM:NF024673.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            1143..1757
                     /locus_tag="VII50_00905"
     CDS             1143..1757
                     /locus_tag="VII50_00905"
                     /inference="COORDINATES: protein motif:HMM:NF033516.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HEY5049428.1"
                     /translation="MADRYHRAPDREGKVYCAVVLDAFSRLVVGWSIDSTQTTTLVLN
                     ALGMATQRRQDRDGLVIHSDRGVQFTSWAFSQNVRDAGIAPSMGAVGSAFDNAMMESF
                     WGRVQVELLNRKKWKTRIELATAIHDYIELFHNTRRRHSGLGMLTPSEVEAGWLKSQD
                     ERLLAKTAPNLPVDGTLLTGLFDAPVEDDHQHHLQLQNNNDRAA"
     gene            1841..2122
                     /locus_tag="VII50_00910"
     CDS             1841..2122
                     /locus_tag="VII50_00910"
                     /inference="COORDINATES: protein motif:HMM:NF013677.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEY5049429.1"
                     /translation="MPRSRKYPTELLDRGARLVFESGRPIAHVAADLGIPAETLRLHV
                     RRVEVDTGRREGLTSEEREEIRKLRKENFELRRANEILKSASVFFGAPG"
     gene            2696..3190
                     /locus_tag="VII50_00915"
     CDS             2696..3190
                     /locus_tag="VII50_00915"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HEY5049430.1"
                     /translation="MLTHRYALRIALSALAIALLTVGLLTHASTAQRRAPALPSRALS
                     GKLTTLADLHGYAAVVLFWAPDCAPCHWEAPAIERFARSARGRGRIVAIDDDYTSAWR
                     KFLRDYDWTFPVLADQTLAVADAYRILGLPTAVFLDPDGRIASSRSGPQTVVSLTRGL
                     SAAA"
     gene            complement(3235..>3455)
                     /locus_tag="VII50_00920"
     CDS             complement(3235..>3455)
                     /locus_tag="VII50_00920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5049431.1"
                     /translation="GQMQYLIGSGKQLAPVWKEWFVGSTRDIGKPDLVGHTALVYGIS
                     ASGKLTTIYPPNFAPSQITHDVPALLAS"
BASE COUNT          607 a         1064 c         1184 g          600 t
ORIGIN      
        1 gtcgggcgtc ggctcgcgct gaaacgaggg gctgcgcgct cgtcttgacc gtcagcaggt
       61 cgagattgac gcgatgcgcg cagggttcgc cgcactgtac gacgccgaca agcaacgcgt
      121 gctcagcgtc gctcggcgcg agctgaccgg atgactctga tcgtcaacgt cgccgcggtg
      181 tgctaggggc gcatgtccga cgcactcgtg ctagccatga gacgcctcct gggggggtcg
      241 gggacgagcg gcaggaccgg ccgcgtcagg agggtcgacg aagcctgcgc tgcagcacct
      301 tctggctgga cacgcaggag tttcgtcgtg aggcgatggt gcgcggagta gcatccttcc
      361 tccctatcga caggacgatc cggagtgagc catcctgggt tccacggagg ccttgatctg
      421 acccgggaaa agtggagtcg gcatgctgga gtctctcggc agagaggatc agcagatgcc
      481 acgcagctat ccaccggagt tccgtcgtca ggtcattgag ctggcgcgtg cgggaacgcg
      541 tgtgaagcag ctcgcggtga cgtttgagat gtcggacgcg acgatctata gctggctcaa
      601 gcaggaccgc atcgaccgtg gcgaggccga gggggcgacg acttcgcagc agctcgagct
      661 ggccgccgcc aagcgccgga tccgacaact cgagaccgag ctggcggtgg ctcgcaaggt
      721 cagtgaggtg ttcctctcgg aggggatcag cccaaaaggc tctacccggt gatcgactct
      781 ctgaccgggc agggcatcaa cgtgcagcag gcgtgcgtcg cgttggctgt gtcgcagtcg
      841 ggctattacg cgtggagggg ccgtgttgac gcgccgagaa cgctgcggcg gatctggctg
      901 gccggggaga tcgctgatgt ccacaaggcg tcgggcggca cttacggggc gctgcgcgtg
      961 accgccgagc tgcgctatgg ccgcgacatt caggtcgggc acaacgcggt ggagttgatc
     1021 atgagggagc tcgggatcaa ggggctaccg acccgtcggc tgccgagagg cgcgaaggtg
     1081 tcgaaggtca cgtcgcttga cctggtcggt cgcgtgttcc gacgcgaccg acccaacgag
     1141 ctgtggctga ccgatatcac cgagcacccg accgtgaggg gaaggtctac tgcgcggtgg
     1201 tgctggacgc gttctcccgg ctcgtggtcg gctggtcgat cgacagcacc cagaccacca
     1261 ccctcgtgct caacgcgctg gggatggcca cccaacgccg ccaggaccgc gacgggctcg
     1321 tgatccactc cgacaggggc gtgcaattca cctcctgggc gttcagccag aacgtccggg
     1381 acgccggcat cgcgccatcg atgggcgccg ttggatcggc gttcgataac gcgatgatgg
     1441 agagcttctg gggacgggtg caggtcgagc tgctgaaccg caagaagtgg aagacgagga
     1501 tcgagctggc gaccgcgatc cacgactaca tcgagctgtt tcacaacacc cgacgccggc
     1561 actccggact ggggatgctc acgccatccg aagtcgaagc cggctggctg aaatcccagg
     1621 atgaacgttt gctcgctaag accgcgccca acctgccagt cgacggaacg ttgcttactg
     1681 gtctctttga cgctccggtt gaagacgacc accaacacca tctacaactc caaaacaaca
     1741 acgaccgagc cgcctgattc cagaaagccg actccaccga aaccaggtca gatcaccttc
     1801 gatgccaggt tttgtctggt ggagggagaa gtctctggtc atgccacgtt cgaggaagta
     1861 ccccacggag ttgttggacc gcggtgcgcg gttggtgttc gagtcgggac ggccgatcgc
     1921 gcatgtcgct gctgaccttg gtatccctgc ggagacgttg cggttgcacg tgcgccgcgt
     1981 cgaggtcgat actggccggc gtgaagggtt gacgagcgag gagcgtgagg agattcgcaa
     2041 gctgcgcaag gagaacttcg agttgcgccg cgcgaacgag atcctgaagt cggcgtctgt
     2101 gtttttcggt gcgccgggat aaaccggcat ggacaaggag atgaaagagc tctacatcga
     2161 aggagtagcg gtccgcggtg gccccgagtc atgcgctggc gctcgcgagg gtgctggcga
     2221 agcgttgaca ggggtacgtg caggctgggc tattgagcct cgaaaacact caattcaggg
     2281 cgccgacgcg gtcgaaaagc gcggaaggcg agatcggcgg tcgcgttatc gcgagcggct
     2341 gctgggctct gcgtggtcga agaacctgtg catgcacgga atctccatgc gcgagaaccg
     2401 ggaggtcccg cgttcgccca gtccggtagg tcgcggcgga gcgccgccgg cgacgccccc
     2461 ttccgccgcg gcccgcccga ccaccacgcc gcgcccgcca ccacgcgccc cgcaggcctg
     2521 gaggagcagg actgtcgagc accgggcggc gtagccggct ggcttggtct ttgtagatca
     2581 gtgaggacac gcggtggagg gagcgtgtcg cgtatggcgg cacactgtgg gaacgtaggt
     2641 cagcgaatat ccttccgccg tcgcgcccac tgcctgagcc ttggatgctg cccgcttgct
     2701 gactcaccga tacgcgctac ggatcgcgct cagcgcgctc gcgatcgcgc tccttactgt
     2761 ggggcttctg acgcatgcct ccaccgctca gcgccgtgcg cccgctctgc ccagccgcgc
     2821 tctcagcggc aagctgacca cgctcgctga cctgcacggc tacgccgccg tggtcctgtt
     2881 ttgggcaccc gactgcgcgc cgtgtcactg ggaagctccg gcaatcgagc ggttcgcccg
     2941 ttccgctcga ggtcggggcc gtatcgttgc aatcgacgac gactacacaa gtgcgtggcg
     3001 caagttctta cgcgactacg actggacgtt ccccgtgctc gcggaccaga cgttggcagt
     3061 cgccgacgcc taccggattc tcggccttcc gaccgcagtg ttcctcgacc ctgacggacg
     3121 tatcgcatcg agtcgcagtg gaccacagac tgttgtcagc ctcacgcgag gactctcggc
     3181 agcagcgtaa agccacccgt ggcgcgggct gctaccgcgc cctaggcggc ttcgtcaact
     3241 tgccagcagt gccggcacgt cgtgagtgat ctggctcggc gcgaagttcg gggggtagat
     3301 cgtcgttagc ttgccgctcg cgctgatgcc gtagacgagc gctgtgtgac cgacaaggtc
     3361 gggtttgccg atgtcgcgag tagagcccac gaaccattcc ttccaaaccg gcgcgagctg
     3421 cttgccggag ccgatcaggt actgcatctg gccga
//
LOCUS       DATCBJ010000042         3723 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_549112, whole genome shotgun sequence.
ACCESSION   DATCBJ010000042 DATCBJ010000000
VERSION     DATCBJ010000042.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 3723)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3723)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3723
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_549112"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1089
                     /locus_tag="VII50_00925"
     CDS             <1..1089
                     /locus_tag="VII50_00925"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit D"
                     /protein_id="HEY5049432.1"
                     /translation="IEKNCEYRNFTQAVTFLTRADYLSPLFTETGYSMAVEKLLGITD
                     EVPVRAQVIRVMMMELNRISSHLVWLGTGGLEIGALSAMLYAFRERELILDIFEMVTG
                     LRMNHAYIRPGGVIQDLPPGAVEKIREFVQLMPGKVDEYSNLLTGQPIWIARLQGVGY
                     LDVNACVALGVTGPLLRAAGLPWDLRKSQPYCGYETYEFDVPTQQEGDCFARFLVRLD
                     EIRESVKIVEQCLDRLRPGPVMVEDKKIAWPAQLALGADGMGNSLDHIRHIMATSMES
                     LIHHFKLVTEGFHVPPGQVYVPIESPRGQLGVHLVSDGGTKPYRVHLREPSFVNLQAV
                     PAVCEGSLIADVIASVASLDPVMGGCDR"
     gene            1086..1880
                     /gene="nuoE"
                     /locus_tag="VII50_00930"
     CDS             1086..1880
                     /gene="nuoE"
                     /locus_tag="VII50_00930"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HEY5049433.1"
                     /translation="MTHSHDEASHSHGEPNGLPTVTLDPRLGPGASLADPMSDDAKAR
                     VRELGAQVIARYPRARSAMMPLLYLVQAEQGYVSPDGIKLVAELLGLTTAEVAAVSTF
                     YTMYKRRTCGDYHVGVCTNTLCAILGGDAIFAALKEHLGVGNAGTTPDNLVTLEHIEC
                     NAACDYAPVVMVNWEFFDNQTVESAKELVDKLRAGDLPVPTRGASLITHVETSRVLAG
                     FPDTLVNEGPSAGPGSLVGLDLAKERGDAAPAIESNAAAAESKDES"
     gene            1877..3181
                     /gene="nuoF"
                     /locus_tag="VII50_00935"
     CDS             1877..3181
                     /gene="nuoF"
                     /locus_tag="VII50_00935"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HEY5049434.1"
                     /translation="MTFALTPVLTANWGTPRSWTLAAYRRTGGYDSLRAALAMEPDAV
                     ISMVKDSGLRGRGGAGFPTGMKWGFIPQGDGKPHYLVVNADESEPGTCKDIPLMMASP
                     HTLLEGVLISAYAIRANEAFIYVRGEVLHVVRRLLNAVEEAKAAGFIGRGVLGSGFDI
                     NVVVHIGAGAYICGEETALLESLEGFRGQPRLRPPFPAIEGLYACPTVINNVETIASV
                     PAIVRGGAAWFRKMGTEKSPGFKIFSLSGHVAQPGQYEAPLGITLRELLDATGGMREG
                     HELKFWTPGGSSTPLFTAAHLDVPLDFEGPVANGSMLGTTALQLFDETTCVVRAVLRW
                     SEFYAHESCGKCTPCREGVFWYVQILERLERGEGDEQDIETLLDLSENILGRAFCALG
                     DGATSPVTSSIQYFRDEYIAHFDGKGCPFDPVASTVFAGASA"
     gene            3181..>3723
                     /locus_tag="VII50_00940"
     CDS             3181..>3723
                     /locus_tag="VII50_00940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HEY5049435.1"
                     /translation="MTVTTNPPAPGGANQPARSDLINLTVDGFEISVPKGTLVIRAAE
                     FVGIQVPRFCEHPLLDPVGACRQCLVEVEGQKKPMASCTTTVTEGMIVKTQYTSAVAE
                     KAQNGVIELLLVNHPLDCPVCDKGGECPLQNQTMTNGYSESRYRDVKRTYPKPIAISA
                     QVLLDRERCVLCTRCTRFAQQ"
BASE COUNT          645 a         1212 c         1186 g          680 t
ORIGIN      
        1 atcgagaaga actgcgagta ccgcaacttc acgcaagccg tcacgttcct cacccgcgcc
       61 gactacttgt cgccgttgtt caccgagact ggctactcga tggcagtcga gaagctgctc
      121 ggcatcaccg acgaagttcc ggttcgtgcg caagtgatcc gcgtgatgat gatggagctc
      181 aaccggattt cctcgcacct ggtctggctc ggcactggcg gtctcgagat cggtgcgctg
      241 tcggcgatgc tctatgcgtt ccgcgagcgc gaactcatcc tcgacatctt cgagatggtc
      301 accggtctgc ggatgaacca cgcgtacatc cggcccggcg gcgtgattca ggatctgccg
      361 cccggcgcgg tcgagaagat ccgcgagttc gtgcaactca tgccaggaaa agtcgacgag
      421 tacagcaacc tgctcaccgg gcagccgatc tggattgccc ggctgcaggg cgtcggttat
      481 ctcgacgtca acgcctgcgt cgcgctcggc gtcaccggtc cgctgctgcg cgccgcgggc
      541 ctgccgtggg acctgcgcaa gagccagccg tactgcggtt acgagacgta tgagttcgac
      601 gtcccgacgc aacaagaagg cgactgcttc gcgcgcttcc tggtgcggct cgacgagatc
      661 cgcgagtcgg tcaagatcgt cgagcagtgc ctcgatcggc tgcgtccagg ccccgtcatg
      721 gtcgaagaca agaagattgc gtggcctgcg cagctcgcgc tcggtgccga tggcatgggt
      781 aactcgctcg accacatccg gcacatcatg gcgacgtcga tggagtcgct catccaccat
      841 ttcaagttgg tcaccgaggg attccacgta cccccgggcc aggtctacgt gccgatcgaa
      901 tcaccacgcg gacaactcgg cgtccatctg gttagtgatg gtggcaccaa gccgtaccgc
      961 gtgcacctgc gcgagccatc gttcgtgaac ctgcaggcgg tcccggcggt gtgcgagggc
     1021 agcctcattg ccgacgtgat cgcgtcggtt gcgagcctcg acccggtgat gggtgggtgc
     1081 gaccgttgac tcacagtcac gatgaagcga gtcacagtca cggtgaaccg aacggcctgc
     1141 cgaccgtcac gcttgatcca cggctcggtc ccggcgcgtc ccttgccgat ccaatgtcgg
     1201 acgacgcaaa ggcgcgcgtt cgcgagctcg gcgcgcaagt catcgcgcgc tacccgcggg
     1261 cgcggtcggc catgatgcca ttgctctatc tcgtgcaagc cgagcaaggt tacgtgtcac
     1321 ccgacggcat caagctggtc gccgaacttc tcgggctgac gacggccgaa gtggccgcgg
     1381 tgagcacgtt ctacacgatg tacaagcgtc gtacgtgcgg cgactaccac gtgggcgtct
     1441 gcacgaacac cctgtgcgcg atccttggcg gcgacgccat tttcgctgcg ctcaaagaac
     1501 atcttggtgt gggcaacgct ggcacgacgc ccgacaactt ggtgaccctc gagcatatcg
     1561 agtgcaatgc ggcttgcgac tacgccccgg tggtcatggt caactgggag ttcttcgaca
     1621 accagaccgt cgagagcgcc aaggaactcg ttgacaagct gcgtgcgggc gacctgccgg
     1681 tgccgacgcg cggtgcatcg ctcatcacgc atgtcgagac ctcgcgcgtg cttgccggct
     1741 tccccgacac gttggtcaac gaaggtcctt ccgcgggtcc cggctcgctg gtcggtctcg
     1801 acctggcgaa ggagcgcggc gacgctgcgc cagcaattga gagcaacgct gcagccgctg
     1861 agagcaagga cgagtcatga ccttcgcgct cacgccggtc ctcacggcga actggggtac
     1921 gccgcggtcc tggacgctgg ccgcgtaccg ccgcacgggc ggttacgact cgctgcgcgc
     1981 ggcactcgcg atggagcccg acgccgtcat ttcgatggtc aaagactccg ggctgcgtgg
     2041 tcgcggcggt gctggcttcc cgaccggtat gaagtggggc ttcatcccgc aaggtgacgg
     2101 caagccgcac tacctcgtcg tcaatgcaga cgagagcgaa cccggcacct gcaaagacat
     2161 tccgctgatg atggccagcc cgcacaccct gctcgagggc gtgctgatct cggcgtatgc
     2221 gatccgcgcc aacgaggcct tcatctacgt ccgcggcgag gtgttgcacg tcgtccgccg
     2281 attgctcaac gcggtcgagg aggcgaaggc ggccgggttc atcgggcggg gcgtgctcgg
     2341 ctcgggcttc gacatcaatg tcgttgtgca catcggcgcc ggtgcctaca tctgtggtga
     2401 agagaccgcg ctgctcgaat cgctcgaagg cttccgcggt cagccgcggt tgcggccccc
     2461 gttccccgcg atcgaaggcc tttacgcctg tccgaccgtc atcaacaacg tcgaaaccat
     2521 cgcgagtgtg ccggcgatcg tgcgcggtgg cgccgcctgg ttccgaaaga tgggcacaga
     2581 gaagtcgccc ggattcaaga ttttctcgct ctccggccat gtcgcgcagc ccggccagta
     2641 cgaggcgccg ctcggtatca ccttgcgcga gttgctcgac gccaccggcg gtatgcgcga
     2701 gggtcacgaa ctcaaattct ggacgccggg gggctcgtcg actccgttgt ttacagcagc
     2761 acacctcgac gtcccgctcg atttcgaagg gccagtggcg aacggctcga tgcttgggac
     2821 aaccgccctt cagctcttcg acgagacgac ctgcgtggtg cgcgcggtgc tgcggtggag
     2881 tgagttctac gcgcacgaat cctgcggcaa gtgcacgccg tgccgcgaag gagtgttctg
     2941 gtacgtgcag atcctcgaac gcctcgagcg tggcgagggt gacgagcagg acatcgaaac
     3001 gctgctcgac ctctcagaga acattctcgg ccgcgcgttt tgtgctcttg gtgacggtgc
     3061 gaccagcccg gtgacctcgt cgattcagta cttccgcgac gagtacatcg cgcacttcga
     3121 cggcaagggc tgtccgttcg atccggtcgc gtcgaccgtc ttcgccggag cgagtgcctg
     3181 atgactgtca cgacgaatcc tcctgccccc ggcggcgcga accagccggc ccggtccgac
     3241 ctgatcaatc tcacggtcga tgggttcgag atttcggtgc ctaagggcac cttggtgatt
     3301 cgggcagctg agtttgtcgg catccaggtg ccgcgctttt gcgagcaccc gctgctcgac
     3361 ccggtcggcg cctgccgtca gtgcctcgtc gaggtcgagg gtcagaagaa gccgatggcg
     3421 tcgtgcacga ccacggtcac cgaaggcatg atcgtcaaga cgcagtacac ctcggccgtt
     3481 gccgaaaagg cgcagaacgg cgtcatcgaa ttgctgctcg tcaaccaccc gctcgactgc
     3541 ccggtgtgcg ataagggcgg cgagtgcccg ctgcagaacc agaccatgac caacggctac
     3601 tccgaatccc gctatcgcga cgtgaagcgc acctatccca aacccatcgc gatctccgcc
     3661 caggtgcttc tcgaccgcga gcgctgcgtg ctgtgcaccc ggtgcacccg cttcgcgcag
     3721 cag
//
LOCUS       DATCBJ010000043         3290 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_288221, whole genome shotgun sequence.
ACCESSION   DATCBJ010000043 DATCBJ010000000
VERSION     DATCBJ010000043.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 3290)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3290)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3290
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_288221"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..546)
                     /locus_tag="VII50_00945"
     CDS             complement(<1..546)
                     /locus_tag="VII50_00945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009083328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HEY5049436.1"
                     /translation="MSKPAGSAQDVRQQAARARTLDELTADSTTCRACPRLVRWREEV
                     ARVKRRSFADEPYWGRPVPGWGDRKARIVIVGLAPAAHGGNRTGRVFTGDRSGDWLFA
                     ALHRAGLAAKPTSVRADDGQRLIGVRMVAAVRCAPPANKPTTDERDTCRPWLVRELEL
                     LAPTLKVLVVLGGFGWQALLPN"
     gene            808..1122
                     /locus_tag="VII50_00950"
     CDS             808..1122
                     /locus_tag="VII50_00950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5049437.1"
                     /translation="MPRQASRIALAAAATVAITLPLTGTASAMSYDRHVVARTAVTVK
                     ATPGHLALARKDVLQAGGQVVRWLPTSNGFVAQVPITAVVGLKSAPGIASVSVLGALI
                     RE"
     gene            complement(1134..2063)
                     /locus_tag="VII50_00955"
     CDS             complement(1134..2063)
                     /locus_tag="VII50_00955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020664780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ppx/GppA phosphatase family protein"
                     /protein_id="HEY5049438.1"
                     /translation="MTRVAAIDCGTNSLRLLIADVDAARGTLVDVDREMNIVRLGQGV
                     DRTRRFADDALERTFAVCREYAERIGAHHVERVRFVATSASRDVTNRDAFVDGVRRAL
                     GVAPEVISGDEEAALSFAGATRELLDVQGMQEPYLVVDIGGGSTEFVVGGRDVEAARS
                     VDVGCVRLTERHLRDDPPTPAQVDAARADIVAAIDLAAQTVPMKSAATLVGLAGSVTT
                     VVALALGLEEYDPVRIHHTRITAAQVHLIAEQLLAMTRAQRAALPVMHPGRVDVIGAG
                     ALVLDTIVARVGAAEVVASEHDILDGIAFSVGD"
     gene            complement(2060..2572)
                     /locus_tag="VII50_00960"
     CDS             complement(2060..2572)
                     /locus_tag="VII50_00960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009649213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF501 domain-containing protein"
                     /protein_id="HEY5049439.1"
                     /translation="MTTPAVSHPDEPTAGDLAVVERQLGRAPRAVRAVAHRCECGLPD
                     VIESAPRLPDGSPFPTFYYLTCPRAAGAVGTLEAAGLMREMSARLQTDGALAAAYDAA
                     HRDYLARRDAVERLPGDPGVGGMPTRVKCLHVLVAHSLVAGPGVNPFGDEALAMVPTW
                     GSAGPCVDAS"
     gene            complement(2569..3180)
                     /locus_tag="VII50_00965"
     CDS             complement(2569..3180)
                     /locus_tag="VII50_00965"
                     /inference="COORDINATES: protein motif:HMM:NF016839.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HEY5049440.1"
                     /translation="MPRRGPAADDDAAPGVGVPPDGDGPRHGKPAFTGRAAILAVVVA
                     ALALAIAAPLRELLSQRSQISSLNSSITSQQQQLAGLQKAQKQWANPTYVEQQARDRL
                     HYVFPGQVPYVTLSPTPVASASAAAAAALASQPWYGRLWSSVQEAAHVAPPKPSPSPS
                     PTLAPALISPPAASAPVITAAPVLTTDPNAAPGATASAGFVGP"
     gene            complement(3183..3281)
                     /locus_tag="VII50_00970"
     CDS             complement(3183..3281)
                     /locus_tag="VII50_00970"
                     /inference="COORDINATES: protein motif:HMM:NF012341.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5049441.1"
                     /translation="MAKYNQLMRIEEELDDAARYAGASAFPRFVVG"
BASE COUNT          533 a         1159 c         1128 g          470 t
ORIGIN      
        1 attcggcagc aacgcctgcc aaccgaaacc acccaggacg accagcacct tcagcgtcgg
       61 cgcgagcaac tcaagttcgc ggaccagcca cggacggcag gtgtcgcgtt cgtcggtcgt
      121 cggcttgttc gccggcggcg cacatcgaac ggcagcgacc atgcggacgc cgatcagccg
      181 ctgcccgtcg tcggcgcgca cgctcgttgg cttggcggca agacctgccc ggtggagcgc
      241 cgcgaacagc cagtcgccgc tgcggtcgcc ggtgaagacg cggccggtgc ggttgccgcc
      301 gtgtgcggcg ggggcgagtc cgacgatcac gatccttgcc ttccgatcgc cccagcccgg
      361 gaccggacgc ccccagtagg gctcgtcggc gaacgaccgg cgtttgacgc gggcgacctc
      421 ttcgcgccag cgcaccagtc gcggacacgc gcggcacgtg gtgctgtcgg cggtcagttc
      481 atcgagggtg cgggcgcgag ccgcctgctg ccgcacatcc tgcgcgctgc ccgccggctt
      541 gctcacgccg cgctcccgtc agatcgtcag cccgcccgtt ctggcgatca tgcacgaagt
      601 aggtaggcga ggccccccgt ctgctaccaa atttgtgcgt gctgatcatg gcgggcaccc
      661 gcccgccgcc atccgctgag ccggatgcgc ggtggcgttg cggcgatcgc ggcgacaccg
      721 cgcctggttg gcggatgggg cggtcggggt ttgttggcga taactcgagt aagccgcaat
      781 gagcggcaac ctcagggggt ccacgccatg ccgagacagg ccagccgaat cgcgctcgcg
      841 gcggccgcga cggtcgcgat cacgttgccg cttacgggta ccgcgtcggc gatgagctac
      901 gaccgccatg tagtcgcgcg gaccgcggtc acggtgaaag caacgcccgg tcacctcgcg
      961 ctcgcgcgca aagacgtgct gcaggccggc ggccaggtgg tccgctggct gccaacgagc
     1021 aatggattcg tcgcccaggt tccgattact gcggtcgtcg gcctgaagag cgcgcccggc
     1081 atcgccagcg tcagcgtcct gggtgcgctg atccgcgagt agcgagccga cgttcagtcg
     1141 ccgacagaga acgcgatccc gtcgagaatg tcgtgctcgc tcgccacgac ctccgccgcc
     1201 cccacccgcg cgacaatcgt gtcgagcacc aacgcgccgg cgccaataac atcgacgcgg
     1261 cccggatgca tgacgggcag cgccgctcgc tgtgcgcgtg tcatggcgag tagctgctcg
     1321 gcgatcaggt ggacctgcgc cgccgtgatg cgcgtgtggt gaatccgaac cgggtcgtac
     1381 tcctcgagac caagcgcgag ggccacgacc gtggtgaccg agccggccaa cccgaccagc
     1441 gtcgccgccg acttcatcgg aaccgtctgc gccgcgagat cgatcgccgc gacgatgtcg
     1501 gcccgcgccg cgtcgacctg cgcaggagtg ggcgggtcgt cacgcagatg ccgttcggtc
     1561 agccgcacgc acccgacgtc gaccgatcgc gcagcctcga catcgcgccc accaaccacg
     1621 aattctgtcg agccgccgcc gatgtccacg acaaggtagg gctcctgcat tccttggacg
     1681 tcgagcagct cgcgagtcgc gccagcgaac gacagtgcgg cttcctcatc gccggagatc
     1741 acctcgggcg caactcccag tgcccgccgc accccatcga cgaatgcgtc ccggttcgtt
     1801 acatcgcgcg acgcgctggt ggccacgaac cgcacccgct cgacgtgatg ggcaccgatc
     1861 cgttcggcgt actcgcggca gacggcgaag gtgcgttcca acgcgtcgtc ggcaaagcgc
     1921 cgagtgcgat cgacaccctg ccccaggcgc acgatattca tctcgcggtc gacgtcgacc
     1981 aatgtgccgc gcgccgcatc gacatcggcg atgagcaatc gcagcgagtt cgtcccgcag
     2041 tcaatcgcgg caacgcgcgt catgacgcgt cgacgcacgg gcccgcgctg ccccacgtcg
     2101 gcaccattgc gagcgcctcg tcgccgaacg ggttcacgcc ggggccagcg acaagcgaat
     2161 gcgcgaccag tacatggagg cacttcaccc gcgtcggcat gccgccgacg ccgggatctc
     2221 cgggcagccg ctcgacggcg tcgcgacgcg ccaggtagtc gcgatgtgcc gcgtcatacg
     2281 cggcggcgag cgcgccgtcg gtctgcaggc gagccgacat ctcccgcatg agaccggccg
     2341 cctccaatgt gccgacggcg ccggccgcac gcggacacgt caggtagtag aacgtcggaa
     2401 acggcgaccc gtcgggcagt cgcggtgccg attcgatgac gtcgggcaga ccgcactcgc
     2461 atcgatgggc aacggcacga acagcacgcg gcgcgcgacc gagttgccgt tcgacgacag
     2521 cgaggtcacc cgcggtcggc tcgtcggggt gcgaaacagc gggggtcgtc acggaccgac
     2581 gaagccggcc gacgcggtcg caccaggtgc tgcattcgga tctgtggtga gaacgggtgc
     2641 ggcggtgatg acgggcgccg acgccgctgg tggcgaaatc aacgcaggcg ccaacgtcgg
     2701 gctcggtgac ggcgacggct tcggcggcgc gacatgcgcg gcctcctgca ctgacgacca
     2761 cagtcggccg taccacggct gggacgcaag cgcggcggcg gcggcggccg acgccgacgc
     2821 taccggggtc ggcgacaacg tcacgtatgg cacttgccca gggaacacgt agtgcagccg
     2881 gtcgcgcgcc tgctgctcga catacgttgg attggcccac tgcttctggg ctttctgcaa
     2941 gccggcgagc tgctgctgct gactggtgat gctgctgttc agcgaggaaa tctgcgagcg
     3001 ctgactgagc agctcacgca acggcgccgc gatcgcgagt gcaagggcgg caacgacaac
     3061 cgcgaggatc gcggcccgtc cggtaaacgc cggcttgccg tgccgcggcc cgtcaccatc
     3121 aggcggaacg ccgacgcctg gcgcagcatc gtcgtctgcg gccggaccgc ggcggggcaa
     3181 cgtcagccga ccacgaaacg ggggaacgcc gacgcacctg cgtagcgcgc cgcatcgtcg
     3241 agttcttcct cgatgcgcat cagctggttg tacttcgcaa tgcgttcgcc
//
LOCUS       DATCBJ010000044         3239 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_472843, whole genome shotgun sequence.
ACCESSION   DATCBJ010000044 DATCBJ010000000
VERSION     DATCBJ010000044.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 3239)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3239)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3239
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_472843"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..404
                     /locus_tag="VII50_00975"
     CDS             <1..404
                     /locus_tag="VII50_00975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5049442.1"
                     /translation="VLYADEQSFTFMTPQGHMFAGWITFSAERTATGTTNLQAQVLLR
                     ANDPIYELALIFGGHRKENKFWEQTLTSVARHLAVADPVVEANVVCVDSGRQWRHWTN
                     VWHNAAIRSLFQSGARIVTAPFRRLRAKPHG"
     gene            397..1836
                     /locus_tag="VII50_00980"
     CDS             397..1836
                     /locus_tag="VII50_00980"
                     /inference="COORDINATES: protein motif:HMM:NF024842.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEY5049443.1"
                     /translation="MGDAPDAIVVGSGPNGLAAAITLADAGLRVHVYEGAAVPGGGAR
                     TEELTLPGFRHDVCSAAHPLALASPFFRRFNLQARGVRVLEPEVQFAHPLDGGRAGFV
                     VRSVDETADGLGADAAAYRRWCGRSVRHADDVVDTVLSSLRRPPRHPLRVAGFGLAAL
                     EPASLMARHFGTDEMRGLLAGVAAHSMLRLSRPPSGAVALLLTTLGHSVGWPVIEGGS
                     SSLVAAMVSAIEDAGGAVFTDRWVTSLDELPPTRAVLLDVTPRSLIELGGDRLPSAYR
                     RALARFRYGAGVCKVDFALSGPVPWTADACRRAGTVHLGGTFEEVARSEDDVAAGRHP
                     ERPYVLAVQAGVVDPSRAPAGQHALWTYCHVPAGSTVDMSARIQAQIERFAPGFRDLV
                     LAKAVRTAAQEEQHNPNYPGGDIASGAQTLRQTFARPALRWNPYRTGIPGVYLCSAST
                     PPGAGVHGRCGELAAQTALREAFGASRTG"
     gene            complement(1858..2055)
                     /locus_tag="VII50_00985"
     CDS             complement(1858..2055)
                     /locus_tag="VII50_00985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5049444.1"
                     /translation="MGYEQGATAQLDVAARPQVRHAVKKTVSGPTALCGAGRIEIRLL
                     GSFDTTAENACPQCAELLAAI"
     gene            complement(2080..2709)
                     /locus_tag="VII50_00990"
     CDS             complement(2080..2709)
                     /locus_tag="VII50_00990"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HEY5049445.1"
                     /translation="MAGVIAVLWAVQIVNWIDHYWLSRHFGIVAREPSRLPDIFTAPL
                     LHWSFSHIEANSAPLFFLGFFAAYRGIRRFVAVTVLIVVMSGLGSWLFSPTRTVSAGA
                     SGVVFGYFGYVVLRGIVERHVLDVIVGIVVAVSYWSILQGVLPEDPHISWQAHLFGLI
                     GGTVAAVLLRQRPPSGSTTDPRPGKPPAAPAGDPSRAALHKELDDLGLG"
     gene            complement(2762..>3239)
                     /locus_tag="VII50_00995"
     CDS             complement(2762..>3239)
                     /locus_tag="VII50_00995"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HEY5049446.1"
                     /translation="VLLVDIDNFKAVNDRYGHLTGDDVLRGVATGLTEGLRPRDFVGR
                     FGGEEFVILLAGSDLEQARHTAERVRAQVAEVAVAAANRREQVSVTVSVGVAAFRDSG
                     HSVHELLDAADTALYAAKHAGRNCVRVAEGARQQVLDLTADGRRLIDMRAQRESVD"
BASE COUNT          549 a         1072 c         1086 g          532 t
ORIGIN      
        1 tggtgctcta tgccgatgag cagtcgttca cgttcatgac ccctcaaggc cacatgttcg
       61 ccggctggat caccttcagt gccgagcgca cggcgacggg cacgacgaat ctgcaggcgc
      121 aggtgttgtt gcgggcaaac gatccgatct acgagctcgc gttgatcttc ggcggccatc
      181 gcaaggagaa caagttctgg gaacagacgc tcacgtcggt ggcacggcac ctcgcagtcg
      241 cggacccagt ggtcgaggct aacgtcgtgt gtgtcgacag cggccgacag tggcggcact
      301 ggactaacgt gtggcacaac gcagcaatcc gcagtctgtt ccaatcggga gcacgcatcg
      361 tgacagcacc gttccggcgg ttgcgcgcca aaccgcatgg gtgatgctcc ggacgcgatc
      421 gtcgtcgggt cggggccgaa cggcctcgcc gcagcaatca cccttgccga tgccggcctg
      481 cgggtgcatg tgtacgaggg tgccgccgtc ccgggcggtg gcgcacggac cgaagaactg
      541 acactgcccg gatttcgtca tgacgtgtgc tcggcggccc acccgttggc actcgcctca
      601 ccgttcttcc gtcgattcaa tctgcaggct cgcggtgttc gagtactcga gcccgaggtg
      661 cagtttgcgc atccactcga cggggggcgg gctggctttg tcgtgcggtc ggtcgacgag
      721 actgctgacg ggctcggagc cgatgctgcg gcttaccgcc ggtggtgtgg ccgctcggtg
      781 cgccacgccg acgacgtcgt cgacaccgtg ctgtcgagcc tgcgccgacc gccacggcat
      841 ccgctgcggg ttgccgggtt cgggctcgcg gcgttggaac cggcatccct catggcacgg
      901 cacttcggca ccgacgaaat gcgcggactg ctcgccggtg ttgccgcaca ttccatgctg
      961 cggctgagtc gcccgccgag cggcgcggtc gctctgctgc tgacgaccct cggccactcg
     1021 gtcggctggc ccgtcatcga gggcgggtca agcagcctgg tcgcggcaat ggtcagcgcc
     1081 atcgaggacg ccggtggtgc tgtgttcacc gatcgatggg tcacctcgct cgacgaattg
     1141 ccgccgacac gcgcggtgct gcttgatgtc accccgcgct cgctcatcga gctcggcggc
     1201 gatcgcctgc cgtcggcgta tcggcgcgcg ctggcgaggt tccggtacgg cgcaggggtg
     1261 tgcaaggtcg acttcgcctt gtcaggaccc gtgccgtgga cggctgacgc gtgccggcgc
     1321 gccgggaccg tgcacctcgg aggcacgttc gaggaggtcg cgcgcagcga agacgacgtc
     1381 gcggcggggc gccaccccga gcggccctac gtgctcgcag tccaagccgg cgtggtcgat
     1441 ccaagtcgcg caccggccgg acagcatgcc ttgtggacct attgccatgt ccccgccgga
     1501 tccaccgtcg atatgagcgc gcggatacaa gcgcagatcg aacgattcgc gcccggcttt
     1561 cgcgacctcg tgctggcgaa ggccgtacgc actgcggcgc aggaggagca gcacaacccg
     1621 aactatccgg gcggcgatat tgcgtcgggt gcacaaacgc tgcggcagac attcgcacga
     1681 cccgcgttgc ggtggaaccc ttatcgcacg ggcattcccg gcgtctactt gtgctcggcc
     1741 tcgactcccc caggggctgg tgtgcacgga cgatgcggtg aacttgcggc gcagacggct
     1801 ctccgcgagg cgttcggcgc aagccgaacc gggtagtgcg gcggccgccg ccagcgccta
     1861 gatggctgct agcaactccg cgcattgcgg acacgcgttc tcggcggtgg tgtcgaaact
     1921 cccgaggagg cggatctcga tccgacccgc accgcagagc gcggtaggcc cggagaccgt
     1981 cttcttcaca gcatgccgaa cctgcggccg ggcagccaca tccagctgag cggtggcgcc
     2041 ttgctcatat cccatgaatt gggatattcc cgagacgggc tatcccaatc ccaggtcgtc
     2101 gagttccttg tggagtgcgg cccgtgacgg atcacccgcg ggggcggccg ggggcttccc
     2161 tggccgcgga tcggtcgtgg atccgctagg cggccgctgc ctcagcagca ctgcggcgac
     2221 ggtcccgccg atgaggccga ataggtgtgc ctgccagcta atgtgcggat cttcgggcag
     2281 cacaccctgc aggatcgacc agtacgacac agcaacgaca atgcccacga tgacgtcgag
     2341 cacgtggcgc tcgacgatcc cgcgcagcac cacgtagccg aaatagccga aaacaacgcc
     2401 actcgcccct gcgctgacgg tccgggtcgg cgagaacaac cagctgccga ggccgctcat
     2461 caccacgatc agcaccgtca ccgcgacgaa tcgccggatc cctcgatacg cggcgaagaa
     2521 ccccagaaag aacagcggtg cggagttggc ttcgatatgc gagaagctcc agtgcaagag
     2581 cggagcagtg aagatatcgg gcaacctcga gggttcgcgc gcgacgatcc cgaaatgccg
     2641 gctcagccag tagtggtcga tccagttgac gatctgcacg gcccacagca ccgcgatcac
     2701 accagccatg acgaaaagcg cacgtctcgc gtcggacaga gcgcgatcag agcggggcac
     2761 gctagtcgac ggattcgcgc tgggcgcgca tgtcgatcag tcgccgaccg tctgcggtga
     2821 gatcgagaac ctgctggcga gcgccttcag cgacgcgcac gcagtttcga cctgcgtgct
     2881 tcgcggcgta cagcgcagtg tcggccgcat cgagcaactc atggacggag tggccgctgt
     2941 cgcggaatgc tgccacaccc acggagacag tgacgctgac ctgttcgcgg cgattggccg
     3001 cggcaacggc gacctccgcg acttgcgccc tgacgcgttc tgctgtgtgg cgggcttgtt
     3061 cgagatcgga accggcgagc aggatgacga actcctcgcc gccgaaccgg ccgacaaagt
     3121 cgcgcggacg caggccttca gtcagccctg tcgccacacc tcgaagcaca tcgtcaccgg
     3181 tgaggtggcc gtaccggtcg ttgaccgcct tgaagttgtc gatgtcgacc agcagcacc
//
LOCUS       DATCBJ010000045         3316 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_68412, whole genome shotgun sequence.
ACCESSION   DATCBJ010000045 DATCBJ010000000
VERSION     DATCBJ010000045.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 3316)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3316)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3316
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_68412"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1285
                     /locus_tag="VII50_01000"
     CDS             <1..1285
                     /locus_tag="VII50_01000"
                     /inference="COORDINATES: protein motif:HMM:TIGR00350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HEY5049447.1"
                     /translation="GVIKPGGATAADGAENVLLVGSDSRTGAGDQFAQAPKGQAPVEG
                     QRSDTVILAHLAKGNKAATLVSLPRDSWVTIPAYTDAAGVAHPAHLDKLNAAFSLGGA
                     PLLVSTVQGLTGIHIDHYVEIDFAGFQNMVNALGGVDICLSQPAHDVQTGLNLTAGAH
                     HLNGTTALEFVRQRYGLPLGDIDRIKRQQQFLASMMRKVESAGTLANPIKLNDFLDAL
                     TKSISVDSGMSASDLAKLALKLKGLGTGNVVLTTMPLTGFSTQNGVDVDDIDVAKSTA
                     LFQSLAVDAPVVLPSAPAAPTPVTLTVPVSAVHVEVFNGGGLSGLARKAASDLTKLGF
                     QLVGTPADRITGASASVIQYGPTQAEAARTLQAAIPGATLQANPQLNTTLQLIVGKSY
                     NGAQAPGTTGVAVPVVTPTEDALAETTAANASCTA"
     gene            1393..2952
                     /locus_tag="VII50_01005"
     CDS             1393..2952
                     /locus_tag="VII50_01005"
                     /inference="COORDINATES: protein motif:HMM:TIGR00350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HEY5049448.1"
                     /translation="MAPRLGRPKVLDPAGEQTAYEHAQRRLRIIGRLIVGFFALIVLA
                     GGIGGVAYQKIFSKVDYGKPLALVLPAAERPAKAVPKPNATTGAATTSGTAKNILILG
                     VDSRLGENAQFQVKGAPAQTEDLSDTAILAHLSADGKNVTLVSIPRDSVVQIPSCEKV
                     DNDGNPILSSTGQLQYSKPTKALFNQAIELGGPYCSAKTLEGLTGVRIDNFLEIDFTG
                     VVKMSKAIGGVPLNICEAIHDSNTGLNLTAGPVNLIGDQALAFVRARYGLTGGDDLHR
                     IQRQQQFMASMVRKALSSANFFNPATMYNFYSAVAGSLSTDMTSSQLLSLGLNYRHIN
                     TSNIIFTTVPTYPAPKGDPFYQHLYWSVDEANALFADIHDDIPIPVSTGTNVGDITLP
                     RSAVTVEVLNGTSTDGLARQVRDALTTAGFDVVSIGTATNSPVAKTTVSYVASHTTSM
                     QTLAGALTHTPQEVLDSSAGANIVLTLGADWAGVSTTAPASSSAPASASPSAIPGVKA
                     TSATTNNCVQG"
     gene            complement(2991..>3316)
                     /locus_tag="VII50_01010"
     CDS             complement(2991..>3316)
                     /locus_tag="VII50_01010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hotdog domain-containing protein"
                     /protein_id="HEY5049449.1"
                     /translation="FVVPVHVGDIVTCDAQINWTGRTSCEVGVRVTAQPWDEAGDVGR
                     HVASAYLVFVAIDDDERPRTVPPVMPENDEDRRRFREAEIRRGTRLARREAILRSRER
                     EARQL"
BASE COUNT          621 a         1171 c         1019 g          505 t
ORIGIN      
        1 gggcgtcatc aagcccggcg gcgcgactgc cgccgacggg gctgagaacg tcctgctcgt
       61 cgggtccgac agccgaaccg gcgccggcga ccagttcgcg caggcaccga agggccaggc
      121 gccggtcgag ggccagcgtt ccgacaccgt gatcctcgcg cacctggcga agggcaataa
      181 ggcggcgacc ctggtgtccc tgccacgcga ctcgtgggtg acgatccccg cgtacaccga
      241 cgccgcgggc gtcgcacatc ccgcgcatct cgacaagctc aacgcggcgt tctccttggg
      301 cggggcgccg ctcctggtca gcaccgtgca ggggctgacc ggcatccaca tcgaccacta
      361 cgtcgagatc gacttcgcgg gtttccagaa catggtcaac gcgctaggcg gcgtcgacat
      421 ctgcctctca cagccggcac acgacgtcca gaccggactg aacctgaccg cgggcgcgca
      481 ccacctcaac ggcacgacgg cgttggagtt cgttcgccag cgctacggct tgccgctcgg
      541 cgacatcgac cgcatcaagc ggcagcaaca gttcctggcg tcgatgatgc gcaaggtcga
      601 gagcgccggc actctcgcca accccatcaa gttgaacgac ttcctcgacg cgctgacgaa
      661 atcgatcagc gtcgactccg gcatgagtgc gagcgatctc gccaagctgg cgctgaagct
      721 gaagggcctg ggtaccggca acgtcgtgct gacaacgatg cccctgaccg gcttcagcac
      781 acagaacggc gtcgacgtcg atgacatcga tgttgcgaaa tcgaccgcgc tcttccagtc
      841 acttgcggtc gatgcgcccg tcgtcttgcc gagcgcgccg gccgcaccta cgccggtaac
      901 gctaaccgtg cccgtcagcg ccgtccatgt cgaggtgttc aacggcggcg gcctgagcgg
      961 cctcgcacgc aaggcggcgt ccgatctgac gaagctcggc ttccagcttg tcgggacgcc
     1021 agccgaccgc atcaccggtg cgagcgccag cgtcatccag tacgggccga cgcaggcgga
     1081 ggctgctcgc acgttgcaag cggcgatccc cggcgcgacg ttgcaggcga acccgcagct
     1141 gaacaccacg ctgcagctca tcgtcggcaa gtcctacaac ggtgcgcagg cgcccggcac
     1201 gaccggcgtg gcagtgcccg ttgtgacacc cacggaggat gccctcgcag aaaccacagc
     1261 tgcgaacgca tcgtgcacgg cctgaccggg aaccaacggc tcgtacccga ccgttacgat
     1321 gggagcgcgg tcgggccgcg ctgatgccgg caggctctga gacgaccgac ttaccaggag
     1381 aatcagcact ctatggctcc gcgactaggt cgccccaagg tgctcgaccc cgcgggcgag
     1441 cagactgcct atgagcatgc ccagcgtcgt ctgcgcatca ttggtcgcct catcgtcgga
     1501 ttcttcgcgc tgatcgtgct cgccggtggt atcggcgggg tcgcctacca aaagattttc
     1561 agcaaggtcg actacggaaa gccgctggcg ctggtccttc ccgccgccga gcgacctgct
     1621 aaggccgtgc cgaagcccaa cgcgaccacc ggagcggcga cgacgtcggg aacggcgaaa
     1681 aacattctca tcctcggcgt cgacagccgg ctcggcgaga acgcgcagtt ccaggtgaaa
     1741 ggcgcgcccg cacaaaccga ggacctgtcc gataccgcga tcctggctca cctctccgct
     1801 gacgggaaga acgtcacgct ggtcagcatt ccgcgcgact ccgtcgtcca gatcccgtcg
     1861 tgcgaaaagg tcgacaacga cggcaatccg atcctcagct cgaccggtca gttgcaatac
     1921 agcaagccga cgaaggcgct gttcaaccag gcgatcgaac taggtggccc ctactgctct
     1981 gccaagacgc tcgagggcct cactggcgtg cgtatcgaca acttcctcga gatcgacttc
     2041 accggcgtcg tcaagatgtc caaggcgatc ggcggcgtgc cgctgaacat ctgcgaggcg
     2101 atccacgact caaacaccgg cctcaacctc accgccggcc cggtaaacct catcggcgac
     2161 caggccctcg cctttgttcg tgcgcgctac gggctcaccg gtggcgacga cctgcatcgc
     2221 atccaacgcc agcagcagtt catggcgtcg atggtgcgca aggcgttgtc gagtgcgaac
     2281 ttcttcaacc cggccacgat gtacaacttc tactcggcgg tcgcgggctc gctcagcacg
     2341 gacatgacca gcagccagct gctttcgctg ggcctcaact accggcacat caatacctcg
     2401 aacatcattt tcacgacggt gccgacgtac cccgccccga agggcgaccc gttctatcag
     2461 catctgtact ggagcgtaga cgaggcgaac gccctgttcg ccgacatcca cgacgacatt
     2521 ccgatcccgg tcagcaccgg caccaacgtc ggcgacatca cgctgccgcg ctcggcggtc
     2581 acggtggaag tgttgaacgg aacgtcgacg gatggcctgg ctcggcaggt gcgcgacgcg
     2641 ctcaccacag ccggcttcga cgtcgtctcc atcggcaccg cgaccaactc tccggtcgcg
     2701 aagaccaccg tgagctacgt ggccagccac accacatcga tgcaaacgct ggcgggtgcg
     2761 ctaacgcata cgccgcagga ggtactcgac tccagcgccg gagcgaacat cgtcttgacg
     2821 ctcggtgccg actgggcggg cgtgtcgacg acggcgccgg cgtcctcgag cgcaccggca
     2881 agtgcttcac cgtccgccat ccccggggtc aaagcgacaa gcgcgacgac caacaactgc
     2941 gtgcaaggct gagcgaagct cagcgccagc agcgggctaa gcgaagccgg ttagagctgc
     3001 ctggcctcgc gctcacgtga tcgcaggatc gcctcgcgtc gggccagccg ggtgccccga
     3061 cgaatctcgg cctcgcgaaa gcgcctgcgg tcctcgtcgt tttccggcat caccggcggc
     3121 acggtgcgcg gacgctcgtc gtcgtcgatg gccacgaaaa ccaggtacgc cgacgccacg
     3181 tgacggccca cgtcgccggc ctcgtcccac ggctgcgccg tgacccgcac accgacctca
     3241 cacgacgtgc gcccggtcca gttgatctgg gcatcgcacg tcacgatgtc gccgacatgc
     3301 acgggaacga cgaact
//
LOCUS       DATCBJ010000046         2639 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_68409, whole genome shotgun sequence.
ACCESSION   DATCBJ010000046 DATCBJ010000000
VERSION     DATCBJ010000046.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2639)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2639)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2639
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_68409"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..196
                     /locus_tag="VII50_01015"
     CDS             <1..196
                     /locus_tag="VII50_01015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5049450.1"
                     /translation="VVAACGWLKLNGLVVHAAGQLGWSGRQDVVRIGVLVATGFAMAL
                     ARNLEVERHQLAVRQGDHHA"
     gene            269..358
                     /gene="kdpF"
                     /locus_tag="VII50_01020"
     CDS             269..358
                     /gene="kdpF"
                     /locus_tag="VII50_01020"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: protein motif:HMM:NF021144.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit F"
                     /protein_id="HEY5049451.1"
                     /translation="MTAENLIGLILAIGVTIYLVIALLMPERF"
     gene            359..2020
                     /gene="kdpA"
                     /locus_tag="VII50_01025"
     CDS             359..2020
                     /gene="kdpA"
                     /locus_tag="VII50_01025"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020661714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HEY5049452.1"
                     /translation="MSATLAGILFLGSLVIALALSYRPLGDYMARVFTAKTHWRAERV
                     MYRAIGVNPDADQKWSTYLRSLLAFSLISVLGLYTMLRAQHHLPFSLGYKGMEQGQSF
                     NTAASFVTNTNWQSYSGESTLGYTAQMVGLAVQNFASAAVGICVAIALVRGFARSRTD
                     QLGNFWVDLIRTVTRILLPMSIVFAVILIAGGAIDNFHATHVVNTIAGNHQSITGGPI
                     ASQEAIKQIGTNGGGFYNANSAHPFENPTNWTNWLQIYFLLVIGFALPRTFGRMVGDK
                     RQGLAIVAVMAVLSILSVIALNVAQGVHHGSVPTSVGAASEGTETRFGVLDSATFASA
                     TTLTSTGAVDSMHDSYTSLGGAITLIDMQLGEVAPGGTGSGLYGILVLATITVFVGGL
                     MVGRTPEYMGKKIAAREMKFSSLYFLTTPAFVLIGTAVAMALPGERAAMENTGAHGLS
                     EVLYAFTSTANNNGSAFAGIGVNTNWYNTVLGIVMLGARFIPMIFVLGLAASLARQQP
                     VPVSEGTLPTHRPLFIGMVVGVVLIIVALTYLPALALGPFAEGLH"
     gene            2020..>2639
                     /locus_tag="VII50_01030"
     CDS             2020..>2639
                     /locus_tag="VII50_01030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009074345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5049453.1"
                     /translation="MTSTALTPPVAMPATHRVQGGFLDPHQLWRSTPDAFRKLNPATL
                     YKNPVMFIVEIGSAFTTWLAIRDHTAFAIITTAWLWLTVLFANLAEAVAEGRGKAQAE
                     TLRRAKTTTMARRVSGWKPGMTVDARSIQEVVASQLVQGDFVVVEAGETIPGDGDVIE
                     GIASVDESAITGESAPVIRESGGDRSSVTGGTKVLSDRIIVRITANP"
BASE COUNT          435 a          840 c          830 g          534 t
ORIGIN      
        1 cgtcgtggct gcgtgcggat ggctgaagct gaacggcctg gtcgttcacg cggccggtca
       61 gctcggctgg tcggggcgcc aggatgtcgt ccggatcggc gtcctcgtcg cgaccgggtt
      121 cgccatggca ctggcccgaa acctcgaagt agagcgacac caactcgccg tgcgccaagg
      181 agatcaccat gcgtgacctg ttcttcatcc tgctcgccct cgcattgttc gccctgtttg
      241 cgctgattgt gcggggggcg gagaagctgt gaccgcggag aatctcatcg gcctgatcct
      301 cgcgatcggc gtcaccatct acctggtcat tgcgttgctg atgccggagc ggttctagat
      361 gagcgcgacc cttgccggca tcttgttctt gggttcgctc gtcatcgcac tggccctgtc
      421 gtaccggccg ctcggcgact atatggcgcg ggtcttcacg gccaagaccc attggcgtgc
      481 cgagcgcgtc atgtaccgcg cgatcggcgt gaaccctgac gccgatcaga agtggtcgac
      541 gtacctgcga agcttgctgg cgttttcact gatatccgtg ctcgggctgt acacgatgct
      601 gcgtgcgcag catcatctgc cgttcagtct cgggtacaag ggcatggagc aagggcaatc
      661 gtttaacacc gctgccagct tcgtgaccaa cacgaactgg cagtcgtact caggtgaatc
      721 gacgctcggt tacacggcgc agatggttgg cctggcagta cagaacttcg catctgcagc
      781 cgtcggcatc tgtgtcgcca tcgctctcgt ccggggcttc gctcgctcgc gtaccgatca
      841 gttgggaaac ttctgggtcg acctgattcg caccgtcacg cggatcctgc tgcccatgtc
      901 gatcgtgttt gcggtgatcc tcatcgcggg cggagcgatt gacaacttcc acgccactca
      961 cgtcgtgaac acgatcgcgg gcaaccatca gtcgatcacc ggcggcccga tcgcctccca
     1021 ggaggccatc aagcagatcg gcactaacgg cggcggtttt tacaacgcga actctgcgca
     1081 cccgttcgag aatccgacca actggacgaa ctggctccag atctacttcc tgctggtcat
     1141 cgggttcgcg cttccgcgca cgttcggtcg catggtgggc gacaagcggc agggtttggc
     1201 gattgtcgcg gtgatggctg tgctgtcgat cctcagcgtg atcgctctca acgtggccca
     1261 gggagtgcac cacgggagcg tgccgacctc ggttggtgcg gcgtcggagg gcacggagac
     1321 gcgcttcggt gtgctcgatt ccgcgacgtt tgcttcggcg acgacgctga cctcaacggg
     1381 cgcggtcgac tcaatgcacg actcctacac gagtttgggc ggcgccatca cgctgatcga
     1441 catgcagctg ggtgaggttg ctcctggcgg taccggtagc ggtctgtacg gcatcctcgt
     1501 cctcgccacg atcaccgttt tcgtgggcgg tctgatggtc gggcgaacac ctgagtacat
     1561 gggcaagaag atcgccgcac gagaaatgaa gttctcctcg ctgtacttcc tgaccacgcc
     1621 ggctttcgtg ctgatcggta ccgcggtcgc aatggcgtta cccggtgaac gcgcggcgat
     1681 ggagaacacc ggcgcgcacg gcctttcgga agtgctgtac gcgttcacct cgaccgccaa
     1741 caacaacggg tcggcgttcg caggcatcgg tgtcaacacc aactggtaca acacggtgct
     1801 cggcatcgtg atgctcggcg cccggttcat tccgatgatc ttcgtgctcg ggttggcggc
     1861 gtcgctcgct cgtcagcagc cggttcccgt ctcggaaggc accttgccta cgcaccggcc
     1921 cttgttcatc ggcatggtcg tcggcgtggt gctgatcatc gtcgctctca cctacttgcc
     1981 agcgcttgca ctcggcccgt tcgcggaagg tctgcactga tgacgtccac tgcgctcacc
     2041 ccaccagtcg cgatgcctgc aacgcatcgc gtgcagggcg gcttcctcga tccccaccaa
     2101 ttgtggcgct cgacgccgga cgcgttccgg aagctcaacc cagcgacgct gtacaagaac
     2161 ccggtcatgt tcatcgtcga aattggctcc gcgttcacca cctggttggc gatccgtgat
     2221 cacacagcgt ttgccatcat cacgactgcc tggttgtggc tcaccgttct cttcgcgaac
     2281 ctggccgagg ctgtcgccga gggacgcggc aaggcgcaag ccgaaacgct gcggcgcgcg
     2341 aagaccacga cgatggctcg ccgcgtcagc gggtggaagc ccggcatgac ggtcgacgcg
     2401 cgcagtatcc aggaggtcgt ggcgagccag ctcgttcaag gcgacttcgt cgtggtcgag
     2461 gctggcgaga cgattcccgg cgacggcgac gtcatcgagg gcatcgccag cgtcgacgag
     2521 tcggccatta ccggcgagtc ggcgcccgtg attcgcgaaa gcggcggcga ccgctcatcg
     2581 gtgaccggcg gcacgaaagt gctctccgat cggatcatcg tgcgcatcac ggccaatcc
//
LOCUS       DATCBJ010000047         5423 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_436069, whole genome shotgun sequence.
ACCESSION   DATCBJ010000047 DATCBJ010000000
VERSION     DATCBJ010000047.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 5423)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5423)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5423
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_436069"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..582)
                     /locus_tag="VII50_01035"
     CDS             complement(<1..582)
                     /locus_tag="VII50_01035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5049454.1"
                     /translation="MAVATVFRMETDLLERRAGADADVVDDVTMLERLASTEPGLFSM
                     MVLEGIDPTKLDFAARLDYAKAWDRQLRSSTAGAQNALAGVLIADLPDDDDVVGLEMN
                     RRADMAAVTMQWPPVTGFGRLDQARRLVTEPPGTFALLSAGEISFRHAQLIADATTGL
                     EPADVLLVEARVLEKSIGQTPAEYGRSARRTERL"
     gene            933..1709
                     /locus_tag="VII50_01040"
     CDS             933..1709
                     /locus_tag="VII50_01040"
                     /inference="COORDINATES: protein motif:HMM:NF013718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEY5049455.1"
                     /translation="MSATSTRLRHLPMRRLFLIVLGAYVVLTAAVVFGSPLDVIDRAA
                     AMSDLAHRFPHATPWVLRYVMLGQRGPSSHVAFVYLVYRALRQRSWRPLVLFVTALTM
                     LNVTVGSVKLATGRLGPALTTHPRAVFDGGDIFPSGHTSNAVVIFGVLAIVAVEHRRA
                     WIALAVFVSTTVGLSTIFLDTHWVTDVLGGWLAGALVLFALPATYALIEQWAFRLILT
                     VTEWFRPSPASWDTAAWDAVPRQQINDLASELLTEDAMYR"
     gene            complement(1684..2823)
                     /locus_tag="VII50_01045"
     CDS             complement(1684..2823)
                     /locus_tag="VII50_01045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional RNase H/acid phosphatase"
                     /protein_id="HEY5049456.1"
                     /translation="MSPRRLIVEADGGSRGNPGPAGYGAVVREASTGEVLAERYEYLG
                     VVTNNVAEYRGLLAGLSAAAEIDPQAYIECRLDSRLVVEQLSGRWQVKHPDLRPLVRD
                     GAALAASFGPGHVTYTWIPRERNKDADRLANRAMDEGTGQPLGAKASGSSSGLRSVAP
                     AELPPSNRIVGWTAGAPPTTLVLVRHGATTFTVEKRFSGVGDPPLIEQGRQQIKAAAA
                     ELAARGGVQRIVSSPLSRCRESAAIIGAALGVPLEVDDDLREVDFGAWEGLTFPVVEE
                     RWPRELALWLGDTSISPPDGESYESLRHRVRIAQERIVNRHRGGTICVVSHSRPIAMI
                     AANLLDTPTGSLYRLHVDNASVSEVDYYDDGPAVLRLFNGTSHLR"
     gene            complement(2820..3524)
                     /locus_tag="VII50_01050"
     CDS             complement(2820..3524)
                     /locus_tag="VII50_01050"
                     /inference="COORDINATES: protein motif:HMM:NF014630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc ribbon domain-containing protein"
                     /protein_id="HEY5049457.1"
                     /translation="MQALDSSLDRLDHRRRTLPELATIDELTARLREVSDALVTAETL
                     ESDLARDQRKAEGDVDQVRQRSDRDQQRLDAGQVSSPRELENLQSEIGSLARRRSDLE
                     DVVLEIMERRELAQSDMSSLAVERDKLTGELDSVTSRRDELFGEIDAEIAASHAARAA
                     VAPDVGAELLALYEKIRGSSDGVGAAALHRARCEGCHLTVNPSELGQMRAAPPDAVLR
                     CEECRRILVRTPDSGL"
     gene            complement(3560..4720)
                     /locus_tag="VII50_01055"
     CDS             complement(3560..4720)
                     /locus_tag="VII50_01055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051540 - metal cluster binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HEY5049458.1"
                     /translation="MPETTLADVVAIMEGLYPPSLAASWDAVGLVCGDPAQRVRRILF
                     AVDPVAVVVDEAIERGADLLITHHPLFLHGTSSVAATTAKGRSVHRLIRAGIALHVAH
                     TNADHANPGVSDALAAALGLVDTRPLEPLPAPQLDKVVTFVPEAHAEKLLDALAAAGA
                     GEIGDYARCAWTAVGVGTFVPRPGATPAIGAVGDVTRVDETRIEMVLPRASRDEVIHA
                     LRAAHPYEEPAFDVLELASWPGDTGTGRVGELPESTTLRDFASTIAAALPSTAGGGRI
                     AGDLGAVVRRVAACGGAGDGYLRQASASGSDVFVTADLRHHVVSEHLADGGCAVVDMP
                     HWATEWPWLGAAADAVRAALTQRGTTVSTDVSTMPTDPWVARVDAGTRKDPH"
     gene            complement(4893..5273)
                     /locus_tag="VII50_01060"
     CDS             complement(4893..5273)
                     /locus_tag="VII50_01060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5049459.1"
                     /translation="MTLTNHKTYEDRIVRLHELVDSTAAPALEGYTFRRCKVSGPVLL
                     WLDPTGSIQIEGCGFNAPDLESILWVNTSQVAFVGVTAVRNCSFISCEMDRVGFGGSP
                     AQISDLRRILSGRVNGSPVPDRRS"
BASE COUNT          927 a         1871 c         1716 g          909 t
ORIGIN      
        1 cagccgctcc gtccgccgtg cactgcgccc gtactcggca ggtgtctgac cgatcgactt
       61 ctcgagcacg cgcgcctcga ccagcaacac gtcggcaggc tcgagaccgg tggttgcatc
      121 ggcgatcagc tgcgcgtgcc ggaagctgat ctcaccggcg ctcagcaacg cgaacgtgcc
      181 cggcggttcg gtcacgagcc gtcgggcttg atcgagccgg ccgaagccag tcactggtgg
      241 ccattgcatg gtgaccgcgg ccatgtcggc acggcggttc atctcgaggc cgaccacgtc
      301 atcgtcgtct ggcaggtcgg caatcaacac cccggcaagc gcgttctgcg caccagccgt
      361 ggacgagcga agctgccggt cccacgcctt cgcgtagtcg aggcgtgcag caaagtccag
      421 cttcgtcggg tcgataccct cgagcaccat catcgagaac agaccaggct cggtgctcgc
      481 gagacgctcg agcatcgtga cgtcgtcgac gacgtccgca tcggcacctg cacggcgctc
      541 gagcaggtcg gtctccatac gaaagaccgt agcgacggcc actgacgaaa cgtttcgagc
      601 atcggaccgg tcacgtacgc gtcacgaacg ggtcacgttg agcgtgccgc ctcgatcaca
      661 agtgcacgtg tcctcaggtg tttctttgct tattaccagg cacttcccag cattgtgcgt
      721 gaaatacatc ggtaactacg gggaagtaaa atccagtgat gcgtgacgag ctcttggctg
      781 atcggccaac cgagacccag gtcggcacca cgacatccat cgcgcacaca gcaggcgtcg
      841 cacacaggtc caacgcacgc acagtgccgg tcgcaagcgt tccccccgcg cgcatgtcac
      901 cgatcgccgg cgtgacggaa ctagcacgca tcgtgtcagc gacatccacg cgcttgcgtc
      961 accttccgat gcgcagactc ttcctcatcg tgctgggcgc atacgtcgtg ctcaccgccg
     1021 cggtcgtgtt cggttcaccg ctcgatgtga tcgatcgcgc tgccgcaatg tcagacttgg
     1081 cgcaccgatt cccgcatgcc actccgtggg tgcttcgcta cgtcatgctc ggccaacgtg
     1141 ggccgtcgtc acacgtcgcc ttcgtgtacc tcgtataccg agccctgcgt cagcgatcct
     1201 ggcggccact cgtgctgttc gtcacggcgc tcacgatgct gaatgtgacg gtcggcagcg
     1261 tgaaactcgc cactggacgg ctcggaccgg ctctgacaac gcacccgcgc gcagtgttcg
     1321 acggcggcga catcttccct tcgggacata cgtcgaacgc cgtcgtcatc ttcggtgtct
     1381 tggcgatagt cgccgtcgag catcggcggg cctggatcgc actcgccgtg ttcgtctcca
     1441 cgaccgtcgg tctgagcacc atcttcctcg acacgcactg ggtgaccgat gtgctcggag
     1501 gctggctcgc cggtgccttg gttctgttcg ccttgccggc gacctacgca ctgattgagc
     1561 aatgggcctt ccgcctgata ctcactgtca cggaatggtt tcgcccgtca cctgcgagtt
     1621 gggacaccgc ggcttgggac gcagtacccc gacagcaaat caatgacttg gcgagcgagc
     1681 tgctcaccga agatgcgatg taccgttgaa cagtcgcaga accgccgggc cgtcgtcgta
     1741 atagtcgacc tcgctcacgc tcgcgttgtc gacgtggagt cggtacagcg aaccggtcgg
     1801 cgtatcgagt aagttggcgg cgatcatcgc gatcggtcgt gaatgactca ccacgcaaat
     1861 cgtccctccg cgatgccgat tcacgatccg ttcctgcgca atgcgcacgc gatgccgaag
     1921 cgactcatag ctctcaccat caggcggtga gatcgaggta tcccccagcc atagcgcaag
     1981 ttcgcgtggc catcgctcct cgacgacggg aaacgtcaga ccctcccacg caccgaagtc
     2041 gacctcgcgc aggtcgtcgt cgacctcgag aggcacacca agcgcagcgc cgataatcgc
     2101 cgcggactca cgacaccgcg ataacggcga ggagacgatt cgctggacgc cgccccgtgc
     2161 cgccaactcc gcagcggccg ccttgatctg ttgccggccc tgctcgatca agggcggatc
     2221 accaacgcca gagaatcgct tctctaccgt gaacgtcgtc gccccgtgcc ggacgagcac
     2281 gagcgtcgtc ggtggcgcac ccgccgtcca gccaacaatg cggttggacg gcggcagctc
     2341 cgcaggagcg acactgcgta aacctgacga cgatcccgat gctttggccc cgagtggttg
     2401 cccggtcccc tcgtccatcg cgcggttcgc caaccgatcg gcgtccttgt ttcgctcgcg
     2461 tggaatccac gtgtaggtca cgtgtccagg tccgaacgac gccgcgagtg ccgcaccgtc
     2521 acggaccaac ggccgcaggt ccgggtgctt cacctgccaa cgtcccgaca gctgctcgac
     2581 gacgagacgt gagtcgagcc ggcattcgat gtaggcctgc ggatcgatct cggccgccgc
     2641 actgagcccg gccagcaggc cgcgatactc ggcaacgttg ttggtgacca caccgaggta
     2701 ctcgtagcgc tccgccaaca cctcacccgt cgacgcttcg cgcacgaccg cgccatagcc
     2761 ggccggtccg gggtttccgc gcgatccgcc gtcggcctca acgatcagcc ggcgtggact
     2821 cacagacctg agtccggagt gcgcaccagg attcgccggc actcctcgca tcgcaacacc
     2881 gcatcaggcg gcgctgcgcg catctgccca agctccgagg gattgaccgt gagatgacaa
     2941 ccctcacagc gcgcgcgatg caacgccgct gcaccgacgc cgtccgacga acctctgatt
     3001 ttctcgtaca gggcaagcaa ttcagcgccg acatcaggag cgacggcggc ccgcgcggcg
     3061 tgactcgcag caatctcggc atcgatctcg ccgaacaact catcgcgtct cgacgtgacc
     3121 gaatcgagtt cgcccgtcag cttgtcacgc tcgaccgcga gtgatgacat gtccgactgc
     3181 gcgagctcgc ggcgttccat gatctcgagc acgacgtcct cgagatcgga cctgcggcgg
     3241 gcgagggacc cgatctccga ctgcaggttc tccagctcgc gaggcgagct gacctgaccc
     3301 gcgtcgagcc gttgttggtc gcgatccgac cgctggcgca cctgatcgac gtcgccctcg
     3361 gctttgcgct ggtcgcgggc gagatcggac tcgagtgttt ccgccgtcac caatgcgtcg
     3421 ctgacttcgc gcagccgcgc ggtcagctca tcgatagtcg ccaactcggg cagggtgcgg
     3481 cgccgatggt cgagccggtc gaggctggag tcgagcgcct gcaagtcgag caggcgggcc
     3541 tggctggcgg gatcggcgtt cagtgcgggt cctttcgtgt tcctgcgtcc acccgagcca
     3601 cccagggatc ggtgggcatg gtcgagacgt ctgtgctcac cgtagtgccg cgttgcgtga
     3661 gcgccgcgcg caccgcatcg gcggccgctc ccagccacgg ccactcggtg gcccagtgcg
     3721 gcatgtcgac gaccgcgcat ccgccgtccg cgaggtgctc agacacgacg tggtgacgta
     3781 gatcagcggt gacaaacaca tccgagccgg acgccgaggc ctggcgcaaa taaccgtcgc
     3841 cggcgccgcc acaggcagcg acccgtcgca ccacggcgcc cagatccccc gcaatccggc
     3901 caccgccggc ggtcgagggc agcgccgcag cgatcgttga cgcgaagtcg cgcagagtcg
     3961 tcgactccgg gagttcgccg acccggccgg tgcctgtgtc gcctggccac gacgccaact
     4021 caaggacgtc gaaggccggc tcctcgtagg ggtgtgctgc tcgcagtgcg tggatcacct
     4081 cgtcgcgact cgcgcggggt agcaccatct cgatgcgggt ctcgtcgacc cgcgtcacgt
     4141 cgccgacggc accgatggcc ggcgtcgcgc cgggcctcgg cacgaaggtt ccgacaccga
     4201 cggccgtcca agcgcaccgg gcgtagtcgc cgatctcgcc cgcgccggcc gccgcgaggg
     4261 catcgagcag cttctcggcg tgggcctcgg gaacgaaggt gacgaccttg tcgagctgcg
     4321 gggcgggcag cggctcgagt gggcgggtgt cgaccagccc gagcgccgct gcaagggcat
     4381 cggacacccc gggattcgcg tgatcggcgt tggtgtgcgc gacgtgcagg gcgatgccgg
     4441 cacggatcag ccggtgcacc gacctcccct tcgcggtcgt tgcagcgacc gacgatgtgc
     4501 cgtgcaggaa cagcgggtga tgcgtgatca gcaggtcggc tccccgctcg atcgcctcgt
     4561 cgaccacgac cgcgacggga tcgaccgcaa acaggatccg gcggacacgt tgcgccggat
     4621 caccgcacac caaccccacg gcgtcccatg acgcggcgag cgatggcgga tacagaccct
     4681 ccatgatcgc cacgacgtcg gcgagcgtcg tctctggcac aagtcagagg ttagcttcca
     4741 ccgcgccgcg gcatcggtcc gtgtggtgtg ggtttggtcg cggcccggac gccgaagcgt
     4801 ccgggccact cgacgcccgg cccggggtga gcggatgcga cgccgtattc agtggacgtt
     4861 tcgacagcag cagaagttgc acgccgagcc ggtcaggagc ggcggtcggg aaccggggag
     4921 ccgttgactc ggccgctcaa gatccttctc aggtcgctga tctgcgccgg actgccgccg
     4981 aagccgaccc gatccatctc acaactgatg aacgagcagt tgcgcacggc ggtcacaccg
     5041 acgaacgcga cctgcgaggt gttcacccac aggatggatt ccagatcggg cgcattgaag
     5101 ccgcagcctt cgatctggat gctgcccgtc gggtcgagcc acagcaggac tgggccgctc
     5161 accttgcagc gccgaaacgt gtagccctcg agggccggcg ccgccgtcga atccaccagc
     5221 tcatggagcc ggacgattct gtcctcatag gtcttgtgat tggttaaagt catcgcgtcc
     5281 cccacggacg tgtcgccctg ttcaaaagcc gtccccaatg ccgattcccc gtcggtcctc
     5341 aagaccctag ggggcaggct gccgcacgac taggcccaac tatcggtgca aagcgggtcg
     5401 aatgggggca ggcgcgcggc tgt
//
LOCUS       DATCBJ010000048         6192 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_472446, whole genome shotgun sequence.
ACCESSION   DATCBJ010000048 DATCBJ010000000
VERSION     DATCBJ010000048.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 6192)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6192)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6192
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_472446"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            274..347
                     /locus_tag="VII50_01065"
     tRNA            274..347
                     /locus_tag="VII50_01065"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:308..310,aa:Trp,seq:cca)
     gene            404..661
                     /gene="secE"
                     /locus_tag="VII50_01070"
     CDS             404..661
                     /gene="secE"
                     /locus_tag="VII50_01070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HEY5049460.1"
                     /translation="MTDTASGAAAPRRAPQAGRVERRNVFSRLGLWWRQIIAELRKVI
                     WPTRKELIAYTTVTLVFAVFMIALVASLDFGANWLVLRVFG"
     gene            891..1448
                     /gene="nusG"
                     /locus_tag="VII50_01075"
     CDS             891..1448
                     /gene="nusG"
                     /locus_tag="VII50_01075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HEY5049461.1"
                     /translation="MRRAPGDWFVVHTYAGYENKAKANLETRIQSLNMEDFIYQIEVP
                     QEEVTEIKNGKRQVVKRNVFPGYLLVRMEMTDESWSAVRYTPAVTGFVGPSSSKPSPL
                     TLDEVFRFLAPATKEQKEAQAAAAPRIVDFEVGESVTVTDGAFATLPASISEINPDQQ
                     KLKVLVSIFGRETPVELSFSQVAKI"
     gene            1505..1936
                     /gene="rplK"
                     /locus_tag="VII50_01080"
     CDS             1505..1936
                     /gene="rplK"
                     /locus_tag="VII50_01080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010058817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HEY5049462.1"
                     /translation="MPPKKKLAAIIKLQIKAGAATPAPPIGPALGQHGVNIMEFCKQY
                     NAATEAQRGNVIPVEISVYEDRSFTFITKTPPAAELIKKAASVDKGSPTPHKVKVASL
                     SKAQLRQIAETKMPDLNARDIEAAEAIIAGTARSMGITIGS"
     gene            2047..2766
                     /gene="rplA"
                     /locus_tag="VII50_01085"
     CDS             2047..2766
                     /gene="rplA"
                     /locus_tag="VII50_01085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013222526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HEY5049463.1"
                     /translation="MMKRSKAYLAAAEKVDADKLYQPIDAIRLARETSLTKNAGTVEV
                     AMRLGVDPRKADQMVRGTVNLPHGTGKTARVLVFATGDRAEEAKAAGADIVGGDELID
                     RVAAGYLDFDSAVATPDLMGKVGRLGRVLGPRGLMPNPKTGTVTNDVAKAVSEIKGGK
                     IEFRVDRQANLHFIIGKTTFTDVQLVENYAAALDEVLRLKPSSAKGRYLRKATVTTTM
                     GPGIQVDPNKTRNLLEDQPVS"
     gene            3052..3564
                     /gene="rplJ"
                     /locus_tag="VII50_01090"
                     /pseudo
     CDS             3052..3564
                     /gene="rplJ"
                     /locus_tag="VII50_01090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005313626.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
     gene            3990..4379
                     /gene="rplL"
                     /locus_tag="VII50_01095"
     CDS             3990..4379
                     /gene="rplL"
                     /locus_tag="VII50_01095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009648085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HEY5049464.1"
                     /translation="MAKLSTDDLIDAFKELTLLELSEFVKQFEDTFGVTAAAPVAVAA
                     AGPAGGGAAVAEVEEQDEFDVILEEAGAQKIQVIKAVRELTSLGLKEAKDLVDGAPKA
                     VVEKGTKEAAEKAKATLEAAGAKVTVK"
     gene            4842..>6192
                     /locus_tag="VII50_01100"
     CDS             4842..>6192
                     /locus_tag="VII50_01100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014525059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5049465.1"
                     /translation="MQVESFDWLLGNDVWKSRVDAATKAGRDDVNLSSGLEEIFEEIS
                     PIEDFSGTMSLSFRDHRFEPAKNSIEECKERDFTYSAPMFVTAEFVNNGTGEIKSQTV
                     FMGDFPLMTDKGTFIINGTERVVVSQLVRSPGVYFDKTIDKTSDKDVFGCKIIPSRGA
                     WLEFEIDKRDAVGVRVDRKRKQSVTVLLKALGWDDARILDRFGQYESMRATLEKDHVH
                     TQDEALIDIYRKLRPGEPPTREAAQTLLDNLYFNPKRYDLAKVGRYKINKKLGLELPF
                     TQGTMTEDDIVATVEYIVRLHAGETEMNLNGRAVPVEEDDIDHFGNRRLRTVGELIQN
                     QVRLGLARMERVVRERMTTQDVEAITPQTLINIRPVVASIKEFFGTSQLSQFMDQTNP
                     LAGLTHKRRLSALGPGGLSRERAGFEVRDVHPSHYGRMCPIETPEGPNIGLIGSLATH
                     "
BASE COUNT         1164 a         2047 c         1961 g         1020 t
ORIGIN      
        1 gcgccgaata cgatgaccgg atgcccggcc gccttcaggg ccttcgcttt cgcgtcgacc
       61 gcgagggtgg cggattcggc gatcgagccg atccggcgtg agaccctgcc gggcgtgtca
      121 gtcatgatgg tgatgatccc atgacagcga ccgtggacgc gctcgggttt cttggaagcg
      181 gttcgaccgt gcgcggcctt gccccgtaca ctgtcgaggc tggtcccctg agctattaag
      241 tggcaagagt ggtaatggtg cttggggcca cgaagggcag tagctcaact ggtcagagtt
      301 ccggtctcca aaaccggcgg ttgggggttc gagtccctcc tgccctgcat gcaccgacca
      361 gctactcaag acaggttttc ctaggtaact gcagggagtc cacgtgaccg acaccgcgag
      421 cggtgctgcc gcaccacggc gtgcgccgca ggctggccgc gtcgagcgcc gcaacgtgtt
      481 ctcgcgtctc ggcttgtggt ggcggcagat catcgccgag ctccgcaagg tcatctggcc
      541 gacccgcaag gagctgatcg cgtacacgac ggtcaccctg gtgtttgcgg tcttcatgat
      601 cgcgctggtg gccagcctcg acttcggggc caactggttg gtcctgcggg tcttcggctg
      661 acgcgccttt cgacccgttc ctgttcgtcg cagtccgccg tcctcaagga agaagagcaa
      721 tccacgtgac cgacttaccg ccgcccgccg accccgatct cgatgtcgag atcgaccttg
      781 acgccgaggt ggccgcggtc gaagcagtcg aagcggctgc ggttgaatcg gctgcggtcg
      841 aatcggcgga gcccgtcgag gtcgatccgg ccgaggcctt ccgagacgcg ctgcgtcgtg
      901 ctccgggcga ctggttcgtc gtgcacacct acgcgggcta tgagaacaag gcgaaggcga
      961 acctcgagac ccggatccag tccttgaaca tggaggactt catctaccag atcgaggtcc
     1021 cccaagaaga ggtcaccgag atcaagaacg gcaagcgcca ggtcgtcaag cgcaacgttt
     1081 tccccgggta tctgctggtc cggatggaga tgaccgacga gtcgtggtcc gcggtccgct
     1141 acaccccggc ggtcactggc ttcgtcggcc cgagttcgag caagccgtcg ccgctgaccc
     1201 tcgacgaggt gttccggttc ctcgcgcccg cgaccaagga gcaaaaggaa gcccaggctg
     1261 ccgctgcccc acggatcgtc gacttcgagg tgggcgagtc cgtcactgtc accgacggcg
     1321 cgttcgccac gctgcctgcg agcatcagcg agatcaaccc cgaccagcag aagctcaagg
     1381 tgctggtctc gatcttcggc cgcgagacgc cggtcgagct ctccttcagt caggtcgcca
     1441 agatctagca ctcccttcga tcccgtactc ttgatcctgc gaatccacca acgaaggacc
     1501 ggtcatgccc ccgaagaaga agctcgcggc gatcatcaag ctgcagatca aggccggcgc
     1561 cgcgacgcct gcgccgccga tcggcccggc gctgggccag cacggcgtca acatcatgga
     1621 gttctgcaag cagtacaacg ccgcgaccga ggctcagcgg ggcaacgtga tcccggttga
     1681 gatctccgtg tatgaagatc gctccttcac cttcatcacg aagacgccgc ccgctgcgga
     1741 gctcatcaag aaggccgcca gcgtcgacaa gggctcgccg acgccgcaca aggtgaaggt
     1801 cgcatcgctg tcgaaggcgc agctgcggca gatcgccgag accaagatgc cggacctgaa
     1861 cgcgcgcgac atcgaagccg cggaagcgat catcgccggc accgcccgct cgatgggcat
     1921 cacgatcggc agttagactc cccaagcccc tgggtggagg tgtttccttc aggggtgttt
     1981 ttagtgtgac gtgggagggc cggcgcggcc cgctgaccac gacctccgat cagattcaag
     2041 gagcacatca tgaagcgcag caaggcgtac ctcgcggcgg ccgagaaggt cgacgccgac
     2101 aagctgtacc agcccatcga cgccatccgg cttgctcgcg agacctccct gaccaagaac
     2161 gccggcaccg tcgaggtggc catgcgcctg ggcgttgacc cgcgcaaggc cgaccaaatg
     2221 gtgcgtggca cggtgaacct gccccacggc accggcaaga cagcccgcgt gctggtcttc
     2281 gccaccggtg accgtgccga ggaggccaag gctgccggtg ccgacatcgt cggaggcgac
     2341 gagctgatcg accgggtcgc cgcgggctac ctcgacttcg actccgcggt cgcgacccct
     2401 gacctgatgg gcaaggtcgg tcgactcggt cgagttctcg gcccgcgcgg gttgatgcca
     2461 aacccgaaga cgggcacggt caccaacgac gtcgcgaagg cggtctccga gatcaagggc
     2521 ggcaagatcg agttccgcgt cgaccggcag gcgaacctgc acttcatcat cgggaagacc
     2581 accttcaccg acgtccagct ggtcgagaac tacgcggcgg ccctcgatga agtgctgcgg
     2641 ctcaagccgt cgtccgcgaa gggccggtac ctgcggaagg ccactgtgac gaccacgatg
     2701 ggtcctggca tccaggtcga cccgaacaag acccgcaacc tgctcgagga ccagcccgtc
     2761 agctgacctc ggcgatggtc gttttgaccg gacggcggga gcgaccgtac tgttgacccg
     2821 ccacagaccg ccggtcggcg tgtgcggccc tcgtttgcga gcctctcggc aagcggcgcg
     2881 ggtcgtgaac tcccgaaggc cccgcaaccg cgggcggccc gcgcaggtac acgaacgtcc
     2941 gacgcctttg cgtcgacacg ccccgtgcgc ctagcgccgg ggcgctcgct tttacagggc
     3001 aactcgaggg acgcatgtcg ccggctccac ccggaaggag gccggcagcc aatgccgagc
     3061 cctgacaagg tgaccgacgt cgccgagttg acggagaact tccgtacctc ggccgcggcc
     3121 gtgctcaccg agtaccgcgg cttgaccgtg tcgcaactca cgcaactgcg gcacaacctc
     3181 gggaccacca cgcaatacac ggtggtcaag aacacgctgt ccaagatcgc ggtgcgcgag
     3241 gccggactgc aggagctcga gagcatgctt tcgggtccgt cggcgatcgc gttcatcaag
     3301 ggtgacaccg tcgaggcggc gaagagtctg cgcgacttcg ctaaggcgaa cccgttcctg
     3361 gtgatcaagg gtggggtgct cgacggcaaa ctggtgactg ccgccgaact ttccaagatc
     3421 gccgacctgg agtcccgcga ggtgctcctt gccaaggtgg ccggtgcagc caatgctgca
     3481 ctgtccaagg ccgcggcgct gttccaggca ccgctcgccc aggtggcccg actggccgag
     3541 gccctgcgcg ctcaggttgc cgagaacacg ccggctgcag cgacacccgc tcccgtcgca
     3601 gaagcgccgg ccgaggtagc agctgtcgcc caggctgccg aaccgactgt cgaggctgcc
     3661 gaaccgactg ctgaggctgc cgagaacacg ccggcttcag cgacacccgc tcccgtcgca
     3721 gaagcgccgg ccgaggtagc agctgtcgcc caggctgctg aaccgactgt cgaggctgcc
     3781 gaaccgactg ctgaggctgc cgaaccggcc gccgaagttg ccgcaccgac gccccaaccc
     3841 gaacccgaac ccgaaaccga acccgaaacc gaacccgaaa ccgaacgcga aaccgaaccc
     3901 gaaccaacgg cgactgacgc cgagtagcag cctgctttgc caaccgtccc cgcacatcgc
     3961 tgcgtttact tgaggaagga cccgccacca tggcgaagct cagcaccgac gacctgatcg
     4021 atgcgttcaa ggagctcacg cttcttgagc tctccgagtt cgtgaagcag ttcgaagaca
     4081 ccttcggcgt caccgcggcc gcgccggtcg cagttgccgc ggctggcccc gcgggcggcg
     4141 gtgcggccgt tgccgaagtc gaggagcagg acgagttcga cgtcatcctc gaggaggccg
     4201 gcgcccagaa gatccaggtg atcaaggccg tccgtgagct cacgagcctc ggcctgaagg
     4261 aagccaagga cctcgtcgat ggcgcgccga aggccgttgt cgaaaagggc accaaggagg
     4321 cggccgaaaa ggcgaaggcc accctcgagg cggccggcgc gaaggtcacc gtcaagtagt
     4381 tgcccgcggt tccacctagt tcgtgtacga agcagcggct gccctcatgc gggcggccgc
     4441 tgtttgtttg tccggagata ggccgggtgg gtgattgtgc acccacaaac cacccgagaa
     4501 gcttgacgtc acggcgtcac agcagcatcc tcgcttgtag ctgtgaataa cttgcacagg
     4561 tcctccaggg accgattggg ccggctcgca agggccgcct cgacagcggc gtgcccatcg
     4621 ggtacgctgc tcgtttgcgc tgtcctctga cgcctgagtg agaactcggg tgctccgtcg
     4681 tcgtgcgcac gccacattgc gagccctgga aggaccccca cttggccgcc tcgcgcagca
     4741 cattgacgtc cggcccccgc acttctggac ctacctatgc accaccccgc gttagcttcg
     4801 ccaagatccg tgaaccactt gaggttccga accttctagc tttgcaggtc gagtccttcg
     4861 actggctcct cggcaacgac gtctggaagt cccgcgtcga cgctgccacc aaggcaggcc
     4921 gcgacgacgt gaacctgtcg tcgggtcttg aggagatctt cgaggagatc agtccgatcg
     4981 aggacttctc cggaaccatg tcgctgtcat tccgcgatca ccggttcgag cctgccaaga
     5041 actccatcga ggagtgcaag gagcgcgact tcacctactc ggcgccgatg ttcgtgacgg
     5101 ccgagttcgt gaacaacggc accggtgaga tcaagagcca gacggtcttc atgggcgact
     5161 tcccgctgat gaccgacaag ggcaccttca tcatcaacgg caccgagcgg gtcgtcgttt
     5221 ctcagctcgt ccggtcgccg ggcgtctatt tcgacaagac gatcgacaag acctccgata
     5281 aggacgtctt cggctgcaag atcatcccga gccgtggtgc ctggctggag ttcgagatcg
     5341 acaagcggga cgccgtcggt gtccgtgtcg accgcaagcg caagcaaagc gtcacggttc
     5401 tgttgaaggc actgggctgg gacgacgctc ggatcctcga ccgcttcggt cagtacgagt
     5461 cgatgcgcgc aacgcttgag aaggaccacg tccacaccca ggacgaagcc ctcatcgaca
     5521 tctaccgcaa gttgcgcccg ggcgagccgc ccacgcgtga ggctgcacag acgctcctcg
     5581 acaacttgta cttcaacccg aagcgctatg accttgccaa ggttggtcgc tacaagatca
     5641 acaagaagct cggtctcgag ttgccgttca cgcaaggcac gatgaccgaa gatgacatcg
     5701 ttgccacggt cgaatacatc gtccggctgc acgcgggcga gaccgagatg aacctgaacg
     5761 gtcgcgcggt tccggttgag gaagacgaca tcgaccactt cggcaaccgt cgtttgcgta
     5821 cggtcggcga gttgattcag aaccaggtac gacttggcct tgctcgtatg gagcgggttg
     5881 tccgcgagcg gatgacgaca caggacgtcg aggcgatcac gccgcagacc ctgatcaaca
     5941 ttcgccccgt ggtcgcgagc atcaaggagt tcttcggaac ttcgcagctc agccagttca
     6001 tggaccagac caacccgttg gctggcctca cccacaagcg tcgcctgtcg gcgcttggtc
     6061 cgggcggttt gtcgcgtgag cgcgctggtt tcgaggttcg cgacgtccac ccgagtcact
     6121 acgggcgcat gtgtccgatc gagactcctg agggtccgaa catcggcctg atcggatcgc
     6181 tcgcgacgca tg
//
LOCUS       DATCBJ010000049        10382 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_107086, whole genome shotgun sequence.
ACCESSION   DATCBJ010000049 DATCBJ010000000
VERSION     DATCBJ010000049.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 10382)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10382)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10382
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_107086"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(9..827)
                     /locus_tag="VII50_01105"
     CDS             complement(9..827)
                     /locus_tag="VII50_01105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011718945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-6-naphthoate synthase"
                     /protein_id="HEY5049466.1"
                     /translation="MTPLSIAFSPCPNDTFAFYAWTHGLIPGAPPVDITFADVDVTNG
                     AAGRGEFDVVKVSYAALPWLLDQYALLPSGGALGRGCGPLVLTAGRTDLRGASVVVPG
                     ERTTAYLLFRLWAADQGVGQISVLPFPEIMPAVRDGKFDAGLVIHEARFTYGNYGLTN
                     VVDLGAWWEADTGLPIPLGAILARRELDAAGLAAAARASVEHAFANPSASAQFVAEHA
                     SEMDPDVQARHIALYVNEFSVNLGDEGYAAISALLNRAAVEGLVPAVDPAALRP"
     gene            complement(824..1516)
                     /gene="mqnB"
                     /locus_tag="VII50_01110"
     CDS             complement(824..1516)
                     /gene="mqnB"
                     /locus_tag="VII50_01110"
                     /EC_number="3.2.2.26"
                     /inference="COORDINATES: protein motif:HMM:TIGR03664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="futalosine hydrolase"
                     /protein_id="HEY5049467.1"
                     /translation="MTILVICAVAAERDAVVRNFGPARDVSASPFTGLAVDTPAGELH
                     AFSVGVGPVAAAIGTAALLTGDYEMVVSAGIAGGFRGRVAIGDVVLANLATYADLGVR
                     VEGGFLTPRDMGIDQDSSLAFGDQRLIDRMEAGPATVLVGQVLTLSAMTGTDADADEL
                     ATRYPRALAEAMEGFAVIAAAGSDHGLRYVAEIRAISNIIGRRDRSTWNIPLAFDALA
                     DAMSTLLNEPLP"
     gene            complement(1513..2967)
                     /locus_tag="VII50_01115"
     CDS             complement(1513..2967)
                     /locus_tag="VII50_01115"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY5049468.1"
                     /translation="MSEPRTTRPPETGDRESVGRKAVHVLAVAGGSAGRWFTRSTRAE
                     GADASGLARLIDLHAVQSAGDALLTVALANTLFFSVAVDQARSRVAIYLLITMAPFSV
                     IAPVIGPLLDRFRNGRRYALAATLILRAFLAWVMAGAVGSKGAFALYPAAFGALVCSK
                     AYGVSRSAVVPRVLPPNVTLVRGNARLTLWGVLTATVAAPIGQGLTWATGTPSWALRL
                     CAVVYLGGSIFAFRLPERVDSSEGEVRLRRRPRRPRPALEDGSRRDAAAPRSTYRRIL
                     PPLRGIGPRMTTMLRAGAGLRAFTGFLTLFLAFLIRRHPLGVFHPGVDLGIIVACAAL
                     GSVLGTTIGGWLKPRQPEILAIVSLFSAAVIGVVTAIWFSLGTAALAILVANISQSLS
                     KLGLDSVIQRDAVEHVRTSAFARSETVLQLSWVLGGFVAILLPSNGSLGLTLGSAVLV
                     VVLVSTMKAVRTRVSPDRGIELLRERRRPAPSDV"
     gene            3025..3996
                     /locus_tag="VII50_01120"
     CDS             3025..3996
                     /locus_tag="VII50_01120"
                     /inference="COORDINATES: protein motif:HMM:NF022674.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3027 domain-containing protein"
                     /protein_id="HEY5049469.1"
                     /translation="MTDTSLDIAVQSVGNGDPGVIPGDIPTMPDAVCAEAVDLAREAA
                     QAVAGQSRVGDHEGVEADAERIVTHFFSSTDRAYANWRWAVTVVRAPRSKHVTVDEVV
                     LLPGSGALLAPPWVPWSDRLQAGDLGIGDVLPTAADDPRLVPGYAGIGPHDQTDDADT
                     FDALVNDLWLGRPRVLSEYGRVEAADRWYDGDHGPQAPIARAADMHCASCGFLTLMRG
                     TLSRVFGICANEYAPDDGRVVSFDHGCGAHSQALVAQTEPLRTPDDAAPEDDVQAIDA
                     FTVDSVVDAPVVDVPAGEILPDDAVLEDMRDATPDDATPDDEGVGTH"
     gene            4032..6905
                     /locus_tag="VII50_01125"
     CDS             4032..6905
                     /locus_tag="VII50_01125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5049470.1"
                     /translation="MLAAWAASPARFREDANAEEDLVLGGYRDRVLIELAQNAADAAS
                     RAAVRGRLRFELNSADAVLRASNTGAPLDAAGVESASTLRASAKRDDPAVGRFGVGFA
                     AVLAVSDEPSMLTNSGSVSWSLARTRADLAPISELADEVTRRGNALPVLRLPYPSELR
                     PAADGFDTEVALPLRDEAAVELVRGLLTDVDAVLLLTLPSLASVEIVLDGTARELTAE
                     PTADGVIVEGTRWIVHETTGRLDPEMMRVRPVEERGRRDFVLTWAVPVDDAGAPVAMP
                     TSVPAVVHAPTPTDEPLTLPALLVAPLPLDPTRRHVAPGPLRDVLLDHAAAAYADLVR
                     ELPSTPDVLALVPVSVAAGAIDGELRARILPLLRDVAFIATAEAEIRMRPANAVLLDD
                     AGLASDEALGDVLVQVLPELVPPAWARRAPTALTALGVRRIQLAALIDELATVDREPS
                     WWHSLYSALANAGVSTDVLAGLPVPLGSGGVARSARGLLMPGDFGDLTVLGLRTIHPD
                     AAHPLLLRLGAVDAEPRAVLRDDNVRAAVENSYDAEQPADVADAVLALVSAAQLQVGD
                     EPWLAELALPADDGELYVAGELLMPQGKLAALVADDAPFGTVDPALVERWGAAVLEAV
                     GVLDSFAVVRDNDVISADHDLDREDEYLDAMRSVLAVDEPVVMSELVGVRDLELVRND
                     AWDAALSLLSQPPLRAAVVEQAVVIGAGLHARVPSYTAWWLREHRVVSGRLATSDPLL
                     MGLFDVVVAAGGVGATGDDEFLIAAGALRDLDDADHDEIAARLGDGDRVVTREQVKAL
                     YARLEPTYAPASVRALRHVELVVVAAGDAVVVDAPDLLALLGNLAAVPASLRNASRVA
                     DALDVSLASELGNFAVVSAGRPGGDHIVHDRLLVADVNGEPQPAAWRVVDGALHVDAG
                     ALDFGLGRGRAWRDGQWSQRYLETELLRGVVPSSLLLAEADLD"
     gene            complement(6833..7135)
                     /locus_tag="VII50_01130"
     CDS             complement(6833..7135)
                     /locus_tag="VII50_01130"
                     /inference="COORDINATES: protein motif:HMM:NF022205.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2530 domain-containing protein"
                     /protein_id="HEY5049471.1"
                     /translation="MAAPRRADPPPFQTNEFAPVIVATGVWLVAFFVLLTQHHSLASH
                     GHGWYVWVGLSGFLLGLWGLSLMLLRRRGIQRAQSRSASASSNDEGTTPRKSSVSR"
     gene            7207..7638
                     /locus_tag="VII50_01135"
     CDS             7207..7638
                     /locus_tag="VII50_01135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011718949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEY5049472.1"
                     /translation="MPCTTATTELASELRVSLMRLARRLRAERLDEGLTPTQLAVLGT
                     LDRHGPMTLGELASHEKVQPPSMTRTTATLEGRGLLTRVTQDLDRRQVRIAISAAGLA
                     LLREDRKRRDAWLAGRFEKLTSDERALLHQVAPLLDRLAQS"
     gene            7662..8936
                     /locus_tag="VII50_01140"
     CDS             7662..8936
                     /locus_tag="VII50_01140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011718950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY5049473.1"
                     /translation="MRNYRLYASGQVVSNTGTWMARVTQDWLVYHILTHDNSFALGFV
                     TALQFLPALAFGMYGGMLGDRYPKRVVLTITQSAMALLSLLSGVLIVMHAMQLWTICV
                     IALVFGVASALDMPVRQAFVIEMVGRDVLQNAVSLNSATFNASRMVGPAVAGLLIEAF
                     GAAPGFFLNAVSYLAVIVGMLAMTKTELHAAPRVARARGQLREGLVYVRNRPELLLPI
                     MLMGFIGTFGFNFQITNALVAQGVFHRGAGSYGILSTAQAVGSLAGALFAARRKQQPR
                     VRLLIIAALVFSVLVAASGFLPSYLLFMVLLLPIGCAGILLATSCNAMLQLSAAPEMQ
                     GRVMALYTTVFVGCAPFGSLLVGWLGGAFGPRWALFAGGVICAVAVVGCALYYVRAQR
                     LEIRVADHVWWSRTLADDELEDEALLADVRAR"
     gene            complement(9210..9611)
                     /locus_tag="VII50_01145"
     CDS             complement(9210..9611)
                     /locus_tag="VII50_01145"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HEY5049474.1"
                     /translation="MLVVDASVLVVALADDGPDGDQARARVRGEQLAAPELLDVEVAS
                     VLRRQVRLGAVAPRRAALALADLAAAPLARAPHQPLLARCWELRDNLTIYDATYVALA
                     EALGVDFLTGDARLANAAGPRCRIEVFRSRR"
     gene            complement(9598..9828)
                     /locus_tag="VII50_01150"
     CDS             complement(9598..9828)
                     /locus_tag="VII50_01150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5049475.1"
                     /translation="MPSVQIKDVPEQTHAVLRQRAAAAHQSLQEYLRARLVEEASRPT
                     LDEILDRAGGRAGGSVPLKAAVSAVRSDRARR"
     gene            complement(9956..>10382)
                     /locus_tag="VII50_01155"
     CDS             complement(9956..>10382)
                     /locus_tag="VII50_01155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5049476.1"
                     /translation="GGCEGIKPGWVRVNFNYFISEPVFTYIVDAVALVARDGWRLLTD
                     YVFDPATGLWRHRQGLVEPPLRLSQVNYEADGRMGYPHHDDRAAESDLASYLAEAVAL
                     FAASAGEEPSDADRPSAALSEDFEHLRWFDLPAQCLTAN"
BASE COUNT         1635 a         3432 c         3584 g         1731 t
ORIGIN      
        1 ttgcagtctc agggtcgcag ggcggcgggg tcgaccgccg ggaccaaacc ttcgacggcc
       61 gcgcggttca acaacgcgga gatggcggcg tagccctcat cgccgagatt gaccgagaat
      121 tcgttgacgt acaaggcaat atgacgcgct tggacgtcgg gatccatctc actcgcgtgc
      181 tccgcaacaa actgcgcgga cgccgagggg ttggcgaatg cgtgctcgac cgaggcccgc
      241 gccgcagccg ccaacccagc ggcgtccaac tctcgtcgtg cgaggatcgc accgagcgga
      301 atgggcaagc cggtgtcggc ctcccaccac gcgccgaggt cgaccacgtt ggtcaagccg
      361 tagttgccgt aggtgaagcg cgcctcgtgg atcaccaggc cggcgtcgaa tttgccgtcg
      421 cgaacagccg gcatgatttc tggaaaaggc aacacgctga tctggccaac accctggtcg
      481 gctgcccata gccggaacag caggtaggcc gtggtccgct cgccgggaac cacgaccgat
      541 gcgccacgca ggtcggtacg cccggcggtg aggacgagcg gtccgcaccc gcgccccagt
      601 gcgccgccgc tcggcagcag cgcgtactgg tcgagcaacc atggcaatgc ggcgtacgac
      661 accttcacca cgtcgaactc gccccgcccg gcggcaccgt ttgtcacatc gacgtcggcg
      721 aacgtgatgt cgacgggtgg tgcgccgggg atcaggccat gagtccacgc gtagaacgcg
      781 aacgtgtcgt tggggcacgg tgagaaggcg atcgagagag gcgtcacggc agcggctcgt
      841 tcagtagcgt cgacatggca tctgcaagag cgtcgaacgc cagcggaatg ttccaggtcg
      901 agcggtcgcg ccgcccgatg atgttcgaga tcgcccggat ctcggcgacg tagcgcagcc
      961 catggtccga gccggcggcg gcaatcacgg cgaagccctc catcgcctca gccagcgcgc
     1021 gcggataccg ggtcgccaac tcgtcggcgt ccgcgtcggt gccggtcatc gccgagagcg
     1081 tcagcacctg gccgaccaac acagtcgccg ggccggcttc cattcgatcg atgagtcgct
     1141 ggtcgccgaa tgcgagcgag gagtcttggt cgatgcccat gtcgcgcggt gtcaggaagc
     1201 cgccttcgac gcgaacgccg agatcggcat acgtcgcgag attggcgagc acgacgtcgc
     1261 cgatcgcgac ccgcccacgg aagcccccgg cgatccccgc actcacgacc atctcgtagt
     1321 cgccggtcag cagcgccgcc gtgccgatcg ccgccgcgac tgggccgacg ccgacgctga
     1381 acgcgtgcag ctcaccggca ggagtgtcga ccgccagtcc ggtgaagggg ctcgccgaga
     1441 cgtcgcgcgc cggcccgaag ttgcgcacga cggcgtcccg ctcggccgcg accgcgcaga
     1501 tgacgaggat cgtcacacgt cgctcggcgc gggtcggcgg cgttcgcgta gtaactcaat
     1561 gccgcggtcg gggctcaccc gagtgcggac ggccttcatt gtcgagacca gcacgaccac
     1621 gagcaccgcg gacccaagcg tcaacccgag gctgccgttc gaaggaagca ggatcgcgac
     1681 gaatcctccg agcacccacg acagctggag caccgtctcc gaccgggcaa atgccgacgt
     1741 gcgcacatgt tcgaccgcgt cgcgctggat caccgagtcg aggccgagct tcgacagcga
     1801 ctgactgatg ttggccacca ggatcgcgag tgcagcagtc ccgaggctga accagatcgc
     1861 ggtcacgaca ccaatcaccg ctgccgagaa caggctgacg atcgcgagga tctcgggctg
     1921 ccgcggtttc agccacccgc cgattgtcgt cccgagcacg ctaccgagcg cggcgcacgc
     1981 cacgatgatg ccgagatcga cgccggggtg gaacacaccg agcggatgcc gcctgatcaa
     2041 gaaggcgagg aagagggtca ggaagccggt gaacgcacgc agtcccgcgc cagcgcgcag
     2101 catcgtggtc atacgcgggc caatcccgcg aagcggcggc aggatccggc ggtaggtcga
     2161 tcggggcgcg gccgcatcgc gacgcgaacc gtcttcaaga gctggccgcg gccgtcgtgg
     2221 tcgccggcgc agtcgaacct cgccctcgct cgaatcgacg cgctcgggca gccgaaacgc
     2281 aaagatgctg ccgcccaagt agacgaccgc gcagaggcgc agtgcccatg acggtgtgcc
     2341 ggtagcccag gtgaggccct ggccgatcgg tgcggcgacg gtcgcggtga gcacgcccca
     2401 cagggtgagc cgggcgttgc cacggaccaa cgtcacgttc ggcggcagga cgcgcggcac
     2461 gaccgcgctg cgcgataccc cgtaggcctt cgagcagacg agtgcaccga aggctgcggg
     2521 gtacagggcg aatgcgccct tggagccgac ggcgccggcc atcacccagg cgaggaacgc
     2581 gcgcaggatc agcgtcgcgg ccagcgcgta gcggcggccg ttgcggaagc ggtcgaggag
     2641 cggaccgatg accggcgcga tgaccgagaa cggcgccatc gtaatgagca ggtagatcgc
     2701 gacccggctg cgcgcctggt cgacggccac cgagaagaac aaggtgttcg caagagcgac
     2761 agtgagcaag gcgtcaccgg cactttgaac ggcatgcagg tcgatcaggc gtgccagccc
     2821 gcttgcgtcg gccccctcgg cgcgcgtcga acgggtgaac caacgaccgg cggagccgcc
     2881 cgccacggcg agcacgtgga cggccttgcg gccgacgctc tcgcggtcgc cggtctcggg
     2941 aggccgggtc gttcggggtt cagacacggg catcagtcaa ctactcacag gccgcgcgcg
     3001 cgatgcatgc gagaatcatg gactgtgacc gacacttcgc tcgacatcgc cgtgcaatct
     3061 gttggtaacg gcgaccccgg tgtcatccct ggcgacatcc ccacgatgcc cgacgcggtc
     3121 tgcgctgagg ccgtcgatct tgcccgtgaa gccgcccagg cggtcgcggg gcagtcgcgg
     3181 gttggcgacc acgagggtgt tgaagccgac gccgagcgga tcgtcacaca cttcttttcc
     3241 agcacagatc gggcgtacgc caactggcga tgggcagtaa ccgtcgtccg cgcacctcgt
     3301 tcgaagcacg tcacggtcga tgaagtcgtg ctgctgcccg ggtctggcgc gttgctcgca
     3361 ccaccttggg tgccctggag tgaccggcta caggccggcg acctcgggat cggggatgtg
     3421 cttccgacgg ccgccgacga tccgcggctg gtgcctggct atgccggcat cggtccccat
     3481 gaccagaccg atgacgccga tacctttgac gccctcgtca acgatttgtg gctcggccgc
     3541 ccccgggtgc tttcggagta cggccgagtc gaggcagccg accggtggta cgacggcgat
     3601 catgggccgc aggctccgat cgcccgggcc gctgacatgc actgcgcgtc gtgcggattc
     3661 ttgacgctga tgcgaggcac gctgagccgc gtgttcggga tctgcgccaa cgagtacgcc
     3721 cccgatgacg gccgggtcgt ctccttcgac cacgggtgcg gagcccactc gcaggctttg
     3781 gtcgcgcaga ccgagccgtt gcgcacaccc gacgacgcag cacccgagga cgacgtgcag
     3841 gcgatcgacg cgttcaccgt tgactcggtc gtcgacgccc cggtggtcga tgtcccggcc
     3901 ggtgagatcc tgccggacga cgcggtgctc gaggacatgc gtgacgcgac gccggacgac
     3961 gcgacgccgg acgacgaggg cgtgggcacg cactgaacga tccgttcggc acggcgggcc
     4021 tgcgggccaa ggtgctggcc gcgtgggccg cctcgccggc ccgcttccgc gaggatgcca
     4081 acgccgagga agacctagtt ctcggcggtt atcgcgaccg cgtcctgatc gagcttgccc
     4141 aaaatgccgc cgatgccgcg agcagggcgg ccgttcgcgg tcggctgcgt ttcgagctga
     4201 acagcgcgga cgccgtgtta cgcgcgtcga acaccggcgc accgctggat gccgccggcg
     4261 tcgaatccgc gtcgaccctg cgggcatcgg cgaaacgtga tgacccggcg gtcggcaggt
     4321 tcggcgtcgg attcgctgcc gtgcttgccg tcagcgacga gccgagcatg ctgaccaact
     4381 ccggttccgt cagctggtcg ctcgcgcgca cgcgcgctga ccttgcgccg atttccgagc
     4441 ttgccgacga ggtgacccgc cgtggtaacg cgcttccggt gctgcgcttg ccctacccga
     4501 gcgagctgcg gcctgcggct gacgggttcg acaccgaggt cgcgttgccc ctgcgcgacg
     4561 aggcagcggt cgagctcgtg cgcggtctgc tcaccgatgt ggacgccgtg ttgttgctga
     4621 cgctgccctc gttggcctcc gtcgagatcg tgttggacgg cacggcgcgt gagctaactg
     4681 cggagccgac cgcagacggt gtgatcgtcg agggcactcg ctggatcgtg cacgagacca
     4741 cgggccgcct cgatcccgag atgatgcgcg tccggccggt cgaagagcgt ggccggcgag
     4801 acttcgtgct cacctgggca gtgcccgtcg acgacgccgg tgcgccggtc gcgatgccca
     4861 cttcggtgcc cgccgtcgtc cacgcaccaa cgcctacgga tgagccgctc accttgcctg
     4921 cgctgctcgt ggctccgttg ccgcttgacc ccacgcgccg gcacgtcgca cccgggccgt
     4981 tgcgtgatgt gctgctcgat cacgcggcgg ctgcgtatgc cgacctcgtt cgcgagctcc
     5041 cgtcgacgcc agacgtgctc gctctcgttc cggtgtcggt ggctgccggg gcgatcgacg
     5101 gcgagctgcg cgcgcgcatc ctgcccctgc tgcgcgacgt cgcgttcatc gcgaccgcgg
     5161 aggccgagat tcgcatgcgg cctgcgaatg cggtactgct ggacgacgcg gggctcgcgt
     5221 ctgacgaggc gctcggcgac gtacttgtgc aggtcttgcc tgagttggtg ccgccggcgt
     5281 gggcacgccg ggcaccgacc gcgctgactg cgttgggcgt gcggcgcatt caactcgcag
     5341 cgctgatcga cgagctggcc actgtcgacc gggaaccgtc gtggtggcac tcgctctaca
     5401 gcgcgctggc aaacgccggg gtctcgacgg atgtgctcgc tggactgccc gtgccgctgg
     5461 gatctggcgg tgtggcgcga tcggcgcgcg gcctgctgat gccgggtgac tttggtgacc
     5521 tgacggtgct cgggcttcgc accatccatc ccgacgccgc ccatccgttg ctgttgcgac
     5581 tgggggccgt cgatgccgag ccgcgcgcgg tgctgcgtga cgacaacgtt cgagcagcgg
     5641 tagagaactc ctacgacgca gaacaaccgg ccgacgtcgc ggacgccgtg ctcgcgctgg
     5701 tttcggcggc gcaactgcag gtcggcgatg agccctggct ggccgagctg gcgctaccgg
     5761 ccgacgacgg cgagctgtac gttgcgggcg aactccttat gccacaagga aagctggccg
     5821 cgctcgtcgc cgacgacgcc ccgttcggaa cggtcgaccc tgcactggtc gagcggtggg
     5881 gtgcggcagt gctcgaggcc gttggcgtgc tcgacagctt tgccgtggtt cgcgacaacg
     5941 acgtcatcag cgccgaccac gatctcgatc gcgaggacga gtatctcgac gcgatgcgat
     6001 ccgttctcgc ggtcgacgag ccggtcgtca tgagcgagct ggtcggcgtc cgtgatctgg
     6061 agctcgtccg taacgatgcg tgggacgccg cactgtcgct gctgtcgcag ccgccacttc
     6121 gggctgcggt cgtcgagcag gcggtggtca tcggcgccgg cctgcacgca cgcgtgccgt
     6181 cctatacggc gtggtggtta cgtgagcatc gcgttgtcag cggccgcctc gcgacgtccg
     6241 accctttgct tatggggctt ttcgacgttg tggttgctgc tggcggagtt ggagctaccg
     6301 gggacgacga gttcctgatc gctgccggcg cgcttcgcga tctcgacgac gccgatcacg
     6361 acgagatcgc ggcccggctc ggtgacggcg atcgcgttgt gacgcgcgag caggtcaagg
     6421 ccctttacgc gcgtctcgag ccgacgtatg cgcctgcgtc ggtgcgggcg ttgcgtcacg
     6481 tcgagcttgt cgtcgtagca gctggcgatg cggtggtcgt cgatgcaccg gatttgctgg
     6541 cgttgctcgg gaatctcgcc gccgtgcccg catcgcttcg caatgcgtcg cgggtcgccg
     6601 acgcgctcga cgtgtcccta gcaagtgagc ttggcaactt cgctgtagtg agcgcagggc
     6661 gtcctggcgg tgaccacatc gtgcacgatc ggttgctggt cgccgatgtc aacggcgagc
     6721 cgcagccggc cgcatggcgt gttgtcgacg gcgcactgca cgtcgacgcc ggtgctttgg
     6781 acttcgggct cggtcgcggc cgtgcgtggc gcgacgggca gtggtcccag cgctacctgg
     6841 agaccgaact cttgcgcggt gtcgtgcctt cgtcgttgct gctggctgaa gccgacctcg
     6901 actgagcccg ctggattccg cgccggcgaa gcagcatgag ggacaggccc cacaagccga
     6961 gcaggaaacc gctgagtccg acccatacgt accagccgtg cccgtggctg gccagtgagt
     7021 ggtgttgggt cagcagcacg aagaacgcca ccagccatac cccggtcgcg acgatgaccg
     7081 gcgcgaactc attggtctga aaaggcggtg ggtcggcacg acgcggcgcg gccatggtcc
     7141 tcaggttatc gccgtccggg gaaacagact aaccattagc aatgctaatg agctacgcta
     7201 acgatcatgc cctgcacgac cgcgaccaca gagcttgcga gcgagttgcg cgtgtcactt
     7261 atgagactgg cccgacgcct ccgggcggag cggctcgacg aagggctgac accaacccag
     7321 cttgcggttc tcggaaccct tgatcggcac gggccgatga ccctcggcga gcttgcatcg
     7381 cacgagaagg tgcagccgcc gtcgatgacg cggacgaccg cgacccttga ggggcggggt
     7441 cttctcaccc gggttactca ggacctcgac cgccggcagg tgcggattgc gatcagtgcc
     7501 gcagggctcg ccttgttgcg cgaggaccgc aagcgccgcg atgcctggct cgcggggcgg
     7561 ttcgaaaagc tgaccagtga cgagcgcgca ctgttgcacc aggtagctcc gctgctcgat
     7621 cgactggcgc agtcttgaaa ggaatgttta gggcgctgtc ggtgcgcaac taccggctct
     7681 acgccagcgg tcaggtggtc tcgaacaccg gcacctggat ggcgcgggtt acacaggact
     7741 ggctcgtcta ccacatcctg acgcacgaca acagtttcgc gctcggcttc gtcacggctc
     7801 tgcaattcct tcctgcgctg gcgtttggca tgtacggagg catgctcggt gaccggtatc
     7861 cgaaacgcgt ggtgctgacg atcacccaga gcgccatggc gctgctgtcg ttgctgtccg
     7921 gcgtgctcat cgtgatgcat gcgatgcagc tgtggacgat ctgcgtgatc gcgctggtgt
     7981 tcggtgtcgc gtcggcgctc gatatgccgg tgcggcaggc cttcgtcatc gagatggtcg
     8041 gccgggatgt tctgcagaac gccgtgagtc tcaactcggc gaccttcaac gcatcacgga
     8101 tggtcgggcc tgctgtcgcc ggcttgctga tcgaagcttt cggcgcagcg ccagggttct
     8161 tcctcaacgc ggtgtcgtac ctcgccgtca tcgtcgggat gctcgcgatg accaagaccg
     8221 agctgcatgc tgctcctcgt gttgcgcggg cacgtggcca gctccgcgag ggccttgtgt
     8281 acgtgcgcaa tcgtcccgag ctgttgctac caatcatgct gatgggcttc atcgggacct
     8341 ttggcttcaa cttccagatc acgaacgcac tggtcgcgca gggcgtgttc caccggggcg
     8401 ccgggagcta tgggattctg tcgaccgcgc aagccgttgg gtctttggcc ggcgcgctgt
     8461 ttgcggctcg ccgcaagcag cagccgaggg tccggctgtt gatcatcgcc gcgctggtgt
     8521 tcagcgtgct ggtggcggcg tccggcttcc tgccgtccta cctgctgttc atggtgctgc
     8581 tgcttccgat cggctgcgcg ggaatcctgc tcgcgacatc gtgtaacgcg atgctgcagc
     8641 tgagtgcggc gcccgagatg caaggacggg tgatggcgct ctacacgacg gtcttcgtcg
     8701 gctgcgcgcc gttcggctcg ctgctggtgg gctggctcgg cggcgccttc ggaccgcgtt
     8761 gggcgctgtt cgccggcggg gtcatctgtg cggtcgctgt tgtgggatgc gcgctgtact
     8821 acgtgcgagc gcagcgcctg gagatcaggg tcgccgatca cgtgtggtgg tcgcgaacgc
     8881 ttgccgacga cgaacttgag gacgaggcgc tgctggccga cgtgcgagcg cgctgacatg
     8941 ttcggggaga cctcccggca tgtcgtgaat ctcgacgcca tcaaccttag cgagaccgcc
     9001 gataccacag cgtgcacaag cccgtcccgg agaccgaccg atggtcgatg aagtccccgg
     9061 caacgccacc ggacttgctg cccgacgccc ctgcggccgt acacgtcatc gctggttgtc
     9121 tgatgccgtc gtgctcttcg gcgcgctcac gggtcaggtc ccggactcga gtaatggcgc
     9181 gggcggtttc ggatgtgctg cgtccggcgt tagcgccttg atctgaagac ttcgatgcga
     9241 cagcgcgggc cggcggcgtt agccagccga gcgtcgccgg taaggaagtc gacgcccaac
     9301 gcctctgcca gtgcgacata cgttgcgtcg tagatcgtca ggttgtcacg cagctcccag
     9361 caccgggcaa gcagtggctg atgcggtgcg cgcgccagtg gagcggcagc gaggtctgca
     9421 agagcaagcg ccgcacgacg aggtgcgacc gcgccgagcc tgacctgcct gcgcagcacc
     9481 gaggccactt ccacgtcgag caactccgga gccgctagct gttctccgcg aacccgcgcc
     9541 cgcgcctgat ccccgtccgg tccgtcatcg gcaagtgcaa cgaccagcac actggcgtca
     9601 acgacgagca cggtcagacc gcaccgccga caccgctgcc ttcaacggaa ccgacccgcc
     9661 tgcccgcccg cctgcccggt cgaggatctc gtccagtgtt ggccggctgg cttcctccac
     9721 cagtcgcgcg cgcagatact cctgcagtga ctggtgggct gctgcggcgc gctgccgcaa
     9781 caccgcgtgc gtctgctcgg gaacgtcttt gatctgaaca ctgggcatgg cgccattatg
     9841 gcgccaccag cgccagatgg caaccacccg actttcggca gacgattgcg tttgtgctgg
     9901 tgagattccg ctcgggctca gagtggctgc gttcgaggga agacgcgtcg tctcgtcagt
     9961 tggctgttag gcattgcgcg ggcaagtcga accaccgaag gtgctcgaag tcttcggaca
    10021 gcgcggccga gggccgatcg gcatccgacg gttcctcgcc ggcgctcgcg gcgaacagtg
    10081 cgacagcctc ggcgaggtag ctcgcgaggt cagactccgc ggcgcgatcg tcgtgatgcg
    10141 ggtaacccat cctgccgtcc gcctcgtagt tgacttggct cagccgaagc ggcggctcga
    10201 ctaatccctg ccggtgccgc cacaacccag tggccgggtc gaaaacgtag tcggtgagga
    10261 gccgccaccc gtcgcgcgcg actagcgcaa cggcgtccac gatgtaggtg aaaaccggct
    10321 cggagatgaa gtaattgaag ttgacgcgca cccaaccggg tttgatccct tcgcagccgc
    10381 cc
//
LOCUS       DATCBJ010000050         2402 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_545668, whole genome shotgun sequence.
ACCESSION   DATCBJ010000050 DATCBJ010000000
VERSION     DATCBJ010000050.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2402)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2402)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2402
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_545668"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(97..1899)
                     /locus_tag="VII50_01160"
     CDS             complement(97..1899)
                     /locus_tag="VII50_01160"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY5049477.1"
                     /translation="MKGAAIAVTAGLLAAACGSSGHKASQATPSAGPSSSAPSASAPA
                     ATTPASSAPASAPASAPASSTPAASAPAGSIAPAAWKQGGTVTIYNEQGQTWPCQFNP
                     FNPAVSSEALGFVYEPLIFVDLLNNSAETPMLATGYQWSADKKSIVFTIRDGVQWSDG
                     QPFSAADVAFTFNLMKKVPALDLYSLWSAAGLQSVTANGNQVTMTFSSVATTYFFNFA
                     DQVGIVPQHIFSAGDPAAHPDTWPDVAPVGTGPFTVSPCTANNIQYVANPHYWMPGRP
                     YIQKVEYPAYLDNNPANLDLASGKAQWGSQFIPNIQQAYLSKSKDNNTWSPPVTNVEI
                     FPNLDPSHKATSTLAVRQAIAAAIDRTQIATIGEGGQQPAANQTGVVVPTFQKYFDSA
                     ALSASGYDKPNVAKATQLMASAGYSTSKPLKLSIITITGYTDWDASLAVIKQELKPIG
                     INLTVNDEAQQTYDDALFNGNFDLAYYGEAGGPSPYYEMRQILDSANTAPIGKQANSN
                     YERYKNPAVDALFQQYPTADDAGQVAIIKQIEAAMIKDVPIIPTTESVDWFQYNTSDL
                     EGWPTKDNPYAQPAAFNVPDGEQVLLHLYSKAAQ"
     gene            complement(1997..>2402)
                     /locus_tag="VII50_01165"
     CDS             complement(1997..>2402)
                     /locus_tag="VII50_01165"
                     /inference="COORDINATES: protein motif:HMM:NF012692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEY5049478.1"
                     /translation="SPAERITLEDVVSGKALAAIAHTEFGTRTSVAEIFERATTELAA
                     ARIVEAFTRELAQHVVNLAIAVDPARIAVGGGLARSWATIAPRLRAALDAAVPFPPDL
                     VLAQFPFDAPLIGALALGVEAAAAVLGEGALR"
BASE COUNT          359 a          741 c          832 g          470 t
ORIGIN      
        1 agccgagccg gcggagtagg tacctcacga cgaatagctc ctcgcgctca ccctcatgga
       61 ccgtccttac acgcgtctac tgctgatcct ttactactac tgagctgctt tggagtacag
      121 atggagcaga acctgctccc cgtcgggcac gttgaacgcg gccggctgcg cgtacgggtt
      181 gtccttggtc ggccagccct ccagatccga ggtgttgtac tggaaccagt ccaccgactc
      241 ggtggtcggg atgatcggca cgtccttgat catggccgct tcgatctgct tgatgatcgc
      301 cacctggccg gcgtcgtcgg ccgtcgggta ctgctggaac agcgcgtcca cggcggggtt
      361 cttgtaccgc tcatagttgc tgttggcctg cttgccgatc ggtgcggtgt tcgcagagtc
      421 aaggatctga cgcatctcgt agtacggcga aggaccgccg gcctcaccgt agtaggcgag
      481 gtcgaagttg ccgttgaaga gcgcgtcgtc gtaggtctgc tgcgcctcgt cgttgaccgt
      541 gaggttgatg ccgatgggct tcagttcctg cttgatcacg gcgagcgatg catcccagtc
      601 ggtgtagccg gtgatcgtga tgatcgacag cttcaacggc ttggaggttg agtagcccgc
      661 gcttgccatc agctgtgtcg ccttcgcgac gttcggcttg tcgtagccgg acgcactcag
      721 agccgccgaa tcgaagtact tctggaacgt cgggacgacg acgccggtct ggttcgccgc
      781 cggctgctgg ccgccttcgc cgatagtcgc gatctgggtc cggtcgattg cggccgcgat
      841 tgcctgccgg accgcgagcg tgctcgtcgc cttatgcgac ggatcgaggt tcgggaagat
      901 ctcgacgttc gtcacgggag gcgaccacgt gttgttgtcc ttcgacttgg acaggtacgc
      961 ctgctggatg ttcgggatga actggctgcc ccattgagcc ttgccgctgg caaggtcgag
     1021 gttggccggg ttgttgtcga ggtacgccgg gtactcgacc ttctggatgt agggccggcc
     1081 tggcatccag tagtgcgggt tggccacgta ctggatgttg ttcgccgtgc acgggctcac
     1141 cgtgaacgga ccggtgccga caggtgccac gtctggccag gtgtccgggt gggccgccgg
     1201 gtcgccggcc gagaagatgt gctgcgggac gatgcccacc tggtcggcga agttgaagaa
     1261 gtaggtcgtc gccacggacg agaaggtcat cgtgacctgg ttaccgttcg cggtcacgct
     1321 ttgcaggcct gcggcgctcc acaacgagta gaggtcgagc gcgggcacct tcttcatcag
     1381 gttgaacgta aatgcgacgt cggccgcgct gaagggctgg ccgtcgctcc actgcacgcc
     1441 atcgcggatc gtgaagacga tcgacttctt gtcggccgac cactggtagc cggtcgcgag
     1501 catcggggtc tcggccgagt tgttcagcag gtcgacgaag atgagcggct cgtagacgaa
     1561 cccgagagct tctgagctga ccgcgggatt gaacgggttg aactggcagg gccaggtctg
     1621 gccctgctcg ttgtaaatgg tgaccgtgcc accctgcttc caggcggccg gcgcgatgct
     1681 gcccgccggt gcgctggcgg ccggcgtgct gctggccggt gcgctcgccg gtgcgctcgc
     1741 gggcgcactg ctcgccggtg tggtcgcggc cggcgcactc gcgctcggag ctgacgacga
     1801 tggtcctgcc gacggagtcg cttggctggc cttgtgtccg ctactaccgc atgcggcggc
     1861 caacaggccg gccgtcactg cgatggcggc acctttcatt cgcctcatcg tgttagcccc
     1921 tcactgcttg cgcggcaggc accgtgcctg tcgggatacg gctgtccgga ctgtcaaaca
     1981 tgggtcgaaa cttttgtcac ctgagcgccc cctctccgag gacggccgcg gccgcctcga
     2041 cacccagcgc cagcgcaccg atgagtgggg cgtcgaacgg gaactgggcg agaacaaggt
     2101 cgggcgggaa cgggacggca gcgtcgagtg cggcacgcaa gcgaggcgcg atggtcgccc
     2161 acgagcgcgc gaggccgccg ccgaccgcaa tgcgtgcggg atccaccgcg atcgccaggt
     2221 tcacgacgtg ctgggccaac tcgcgggtga aggcctcgac gatgcgtgcg gccgcaagct
     2281 cggtggtggc cctctcgaag atctccgcga ccgacgtcct ggtcccgaac tcggtgtgcg
     2341 cgatcgccgc cagggctttg ccgctcacga cgtcctcgag ggtgatgcgc tccgccggcg
     2401 ac
//
LOCUS       DATCBJ010000051         2521 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_398792, whole genome shotgun sequence.
ACCESSION   DATCBJ010000051 DATCBJ010000000
VERSION     DATCBJ010000051.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2521)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2521)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2521
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_398792"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            144..866
                     /locus_tag="VII50_01170"
     CDS             144..866
                     /locus_tag="VII50_01170"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: protein motif:HMM:NF014021.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HEY5049479.1"
                     /translation="MDLSDPTRAVTATLDGTVLAVLAAAGKPLTVGQVAQQAVRGSEI
                     GIRRSLARLVDQGIVRATLMGRNQVHELNRDHIAAGVADALAELRPELWKRLRAELSH
                     WRPHPLYASVFGSAARGDGNESSDIDLLLVHPPFPGEKRPPRPNARLGSQVTEALAEF
                     AAASSEPEAALRWEEQVDRLRGLVQGWTGNALQVVDVSFQDWRHPHSDSEPLFVEVER
                     DGIELMKSRVFTVWPVRRPTRG"
     gene            859..1302
                     /locus_tag="VII50_01175"
     CDS             859..1302
                     /locus_tag="VII50_01175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5049480.1"
                     /translation="MAKARSTRPATLENARVRLRVSEAYLQVAELVLEEPDRTEMPGV
                     SVGIAVLAGIAASNLICARRLGQIHRGQDHRAAAALLEQATPDGKKLAATFIRLIDLK
                     DQAHYGMLTIPRSRPAAHSSGHSYSSTAHDKSSNDKARVHPFHAY"
     gene            1630..>2521
                     /locus_tag="VII50_01180"
     CDS             1630..>2521
                     /locus_tag="VII50_01180"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEY5049481.1"
                     /translation="MARTTVEAWCDRWLEGYATRRPSTVRQAKVHVAQIEAAFGSLPL
                     SAVRPSHVKSWTAKLKADGKSTSYVYALHARLAQVMADAVHDGLLARNPCSRRTSPGA
                     GSQRAYVATTEQVWALHDAMPKHLRIAVLLGAFVGLRKAEACGARVADVDFMRGIVSP
                     TVQSPGEPPKSEMSRTAVPIPNELALELSAAVKQWPATTVVSDGAGGVAAPWAIERAI
                     RTARAKVKGLPAGFRYHDLRHYFASLLISSGADVKVVQARLRHASAKTTLDTYGHLWP
                     GSDESARAAVGVVLAARADSL"
BASE COUNT          433 a          818 c          831 g          439 t
ORIGIN      
        1 gagccaccga ccgatccatc ctcaaatggc cgcgatgatg ctcgtttcgt cgtgccggat
       61 agcccatgca cggaaacggg ggccgagtct cgccgttctt gaaccttggc tatctaaaat
      121 gatcggctgc tatagttttc gatatggacc tatctgatcc gacacgtgcg gtgacggcaa
      181 cgctggacgg caccgtgctg gcggtgctcg ctgcggcagg gaagccgttg acagtgggtc
      241 aggtcgctca gcaggccgtc cggggctccg aaatagggat ccgccgcagc cttgcccgac
      301 tcgtcgatca gggcatcgtg cgcgccacgt tgatgggccg taaccaagtt catgaactca
      361 accgcgatca tattgccgcc ggtgttgccg atgccctggc cgaactcaga cccgagctct
      421 ggaaacgtct tcgcgcggag ctgtctcact ggcgcccaca cccgctgtac gcgagcgtgt
      481 tcggttcggc tgcccgcggc gacgggaacg agtcgagtga catcgacctt ctcctcgttc
      541 acccaccctt ccctggcgag aagcggccgc cgcgcccgaa cgcacgtctc gggtcgcagg
      601 tgacggaggc actcgctgaa ttcgcggcag ccagtagcga acccgaggcg gcactgcgat
      661 gggaggagca ggtcgaccgt ctccgaggcc tggtccaagg atggaccgga aatgccctac
      721 aagtcgtcga cgtctctttc caggactggc gacatcccca cagcgacagc gagccgttgt
      781 tcgttgaggt cgaacgtgat gggatcgagt tgatgaagtc acgcgtattc acggtgtggc
      841 ccgtacgtcg gccgacccgt ggctaaggca cggagcacgc ggccagcaac tctcgagaac
      901 gcgcgcgtac gtctgcgtgt cagcgaggct tatctgcagg tcgccgagct ggttcttgag
      961 gagccagacc ggaccgagat gccaggcgtc tcggtaggga ttgcggtcct ggccggcatc
     1021 gcagcgtcca atttaatttg cgcccgtcgc ctcggccaga tccatcgcgg gcaggatcac
     1081 cgcgccgctg ccgctctgct cgagcaggcc acacctgatg gcaagaaact cgctgcgacc
     1141 ttcattcgat taatcgatct gaaggaccag gcccactacg gcatgcttac cattccccgc
     1201 agcaggcccg cagcgcactc aagtgggcac agctactcgt cgaccgcgca cgacaagagc
     1261 tcgaacgata aggcacgcgt gcaccccttc cacgcctact aggtggtaac cctcgatcgc
     1321 cgtacacatg gcgttagcgt tgcgcgcctc gcagagtgaa atcgcagtga cccgttagcg
     1381 cgcgagtggt gccagtggtg aacgcttcgc tgttcgtgtt gctgagaacg cccgcgttga
     1441 cgccgacgtg cctcccgata ggcccggacg ggtgatcatc gatgggccgg ttggtcgcag
     1501 tggcgcgccc ggtaccgcga cgcagccggc cgagagcact ccaagcactc ccgcgcaagg
     1561 tcgacgcgac gcgttggctc gatcaggtca cccgcggccg ttgtgtccgg cgcctacgtc
     1621 gatccgaagt tggcgcgcac gacggtcgaa gcgtggtgtg accggtggct cgagggctac
     1681 gcgacgcgca gaccatcgac ggttcggcag gcgaaggtcc atgttgcgca gatcgaggcc
     1741 gcgttcggct cgcttccgct gtcggccgtg cgtccctccc acgtcaagtc gtggacggcg
     1801 aagctgaagg ccgacggcaa gtcgacgagc tacgtctacg cgctgcacgc ccgactggct
     1861 caggtgatgg ccgatgcggt ccacgacggg ctactggccc gcaatccatg ctcgaggagg
     1921 acgtcgcccg gcgcagggtc gcagcgtgct tatgtagcga cgacggagca ggtgtgggca
     1981 ctgcacgacg cgatgccgaa gcatctgcgc atcgcggtat tgctcggcgc gttcgtcggg
     2041 ctccggaaag ccgaggcgtg cggggcgcga gtggccgacg tcgacttcat gcgcggcatc
     2101 gtgtcgccga ccgtccaatc gccaggtgag ccgccgaagt cagagatgag ccggaccgct
     2161 gtgccgattc cgaacgagct cgcactcgag ctctccgccg cggtgaagca gtggccggcg
     2221 accacggtcg tgtctgacgg cgccggggga gtcgctgcgc catgggcgat cgagcgtgcg
     2281 atccggacgg cgcgagccaa ggtcaagggc ttgcctgccg gcttccgcta ccacgatttg
     2341 cgccactact tcgcgtcgct gctgatctca tcgggcgccg acgtcaaggt cgtccaggcc
     2401 cgacttcgcc acgcctcggc gaagaccact ctcgacacct acgggcacct gtggcccggc
     2461 agcgatgagt ccgcacgtgc ggccgtcgga gtggtgctgg ctgctcgtgc ggactcactg
     2521 c
//
LOCUS       DATCBJ010000052         4078 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_398646, whole genome shotgun sequence.
ACCESSION   DATCBJ010000052 DATCBJ010000000
VERSION     DATCBJ010000052.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 4078)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4078)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4078
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_398646"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1317)
                     /locus_tag="VII50_01185"
     CDS             complement(<1..1317)
                     /locus_tag="VII50_01185"
                     /inference="COORDINATES: protein motif:HMM:NF012492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEY5049482.1"
                     /translation="MTQTRLEPDQLLAVATRVLHQLAGPSAVLRPDQLVAAQALVCEG
                     RRVLCVQATGWGKSAVYWIAAAALRSAGSGPVLVISPLLALMRDQVAAAQRAGLHAVT
                     LNSANVDDWAAIEKQLTDDEVDVLLTSPERLASTRFAESVLPNLLERLGMLVIDEAHC
                     VSDWGFDFRPDYQRLTRLLTSTRPDLPVLATTATANDRVTRDVAYQLAAGNTVEPLVL
                     RGTLARSSLQLGVVPGLSALQRYAWVAAALDRLDGSGIVYVLTVADTVRLADFLTSRG
                     HAVAAYSGQLDADERERIEDQLQRNEIKTVVSTSALGMGYDKPDLAFCLHVGSPASPV
                     AYYQQVGRAGRALDTAVAVLLPAETDDRVWAYFADASTPDPALAAAVLSALAAASQPL
                     SLPKLEDATGSRRGRLEALLKILAVDGAVRRVGTGWQSTGEPWVFDE"
     gene            1480..3531
                     /locus_tag="VII50_01190"
     CDS             1480..3531
                     /locus_tag="VII50_01190"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEY5049483.1"
                     /translation="MASPAEETPNRGWTAWWAYLTLIFIGSGSLIGLSARALTHDHLH
                     GSASAFALVAALLVLGELRPLVASGSYDPDGVSLSTAFVFAILLIWGPAPAIILQFAG
                     ISMREVLMRKAWWRVIFNAGQYAICLATASVVLRLFGISPSLAHPHEVTGGTLLGLVV
                     AAIVYHLMNLLLVGIIISIRRGTTLWAEIIDEFWYYTSTTMAVIALSPVIAVVAVHAW
                     QLIPLLILPLFLVYKTASISLEREQQSLHDALTGLANRKLLMARTGELLDELQLTDGR
                     VALVLLDLDRFKDINDTLGHPVGDRVLQVVAARVQDAVRPGDLVARLGGDEFAILLHD
                     LSDEATVIEVAERIRATLHEPISFDGTMVDLDASLGIALFPDHGDDFDQLHRRADVAM
                     YVAKSSGGGVAVYDIEKDTNSLTRLGLLGELRHAVEVGGLELWYQPKVSLATGEVLGV
                     EALVRWHHPTLGLVMPDQFIPLAEQSGLMPRLTDLLLDQALAVRAQLDAGGSGLVLAV
                     NISVRDLLDFGFADRVAAALRRHHVAPETLMLEITERRLLADYDRGAKVLSELVASGV
                     HISLDDFGTGYSSLTMLKRVPVCELKIDQSFVSRIGDSEADATIVGSIVELAHALNLT
                     VVAEGVETTDVLERLKALGCDQAQGWLVGLPMVAATASAWIARHRRDTQGARAPLRAL
                     S"
     gene            3637..3846
                     /locus_tag="VII50_01195"
     CDS             3637..3846
                     /locus_tag="VII50_01195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5049484.1"
                     /translation="MIRRPLPGSPFNVITVARPVEVFAVNDRVSHDRYGLGCVIGVEP
                     DVAVLVDFGPRQERLVAPYPRLTKL"
     gene            3846..>4078
                     /locus_tag="VII50_01200"
     CDS             3846..>4078
                     /locus_tag="VII50_01200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5049485.1"
                     /translation="MRTLFANGSVFDGTGAPAVRGAVVIEGDRIVEVGQGNGNEMDAD
                     VRLDLAGAAVVPGFFDCHTHVTVSGFDYMSLLQR"
BASE COUNT          673 a         1307 c         1365 g          733 t
ORIGIN      
        1 ctcgtcgaag acccacggct cgccggtcga ctgccatccc gtcccgaccc gcctaaccgc
       61 gccgtcgaca gccaggatct tgagcaacgc ctcgagccgg ccgcgccttg agccggtagc
      121 gtcctcaagc ttgggcagcg aaagcggctg cgacgcggca gccaacgccg acaacaccgc
      181 tgccgccagt gccggatcgg gcgtcgatgc gtcggcgaag taggcccaga cccggtcgtc
      241 ggtctcggcg gggagcagga ccgccaccgc ggtgtcgagg gcgcgaccgg ctcgcccgac
      301 ttgctggtag taggcgaccg gcgatgcggg tgatccgacg tgcaggcaga acgcgaggtc
      361 aggcttgtcg tagcccatgc ccaatgcgga ggtcgagacg acagtcttga tctcgtttcg
      421 ctgcagctgg tcctcgatac gttcacgttc atcggcgtca agctggcccg aatatgccgc
      481 gacggcgtga ccacgcgagg tgagaaagtc agcaaggcgg accgtgtctg cgacggtgag
      541 cacgtagacg atcccggacc cgtcaagccg gtcgagcgcg gcggcgaccc atgcataccg
      601 ttgcaaagcc gaaagccccg ggacgacgcc gagctgcagc gagcttcggg ccaaggttcc
      661 gcgcaacacc aacggctcga cggtgttgcc cgccgcgagc tgataggcga cgtcgcgggt
      721 gacccgatcg ttggcggttg ccgtcgtggc cagtacgggc aggtcggggc gcgtgctggt
      781 gagtagccgg gtcagtcgct ggtagtcggg acgaaagtcg aatccccagt cggatacgca
      841 gtgcgcttcg tcgatgacga gcatgccgag gcgttcgagc aggttcggca acaccgactc
      901 tgcgaagcgc gtggacgcga gtcgctccgg ggaggtgaga aggacatcga cctcgtcgtc
      961 ggtcagctgc ttttcgatgg ccgcccagtc atcgacattg gccgagttga gcgtgaccgc
     1021 atgcaggccg gcgcgctgcg ctgcggcgac ctgatcgcgc atcagcgcga gcaagggaga
     1081 gatgaccagg accggaccgg atccggcgga ccgcagcgcg gcggccgcga tccagtagac
     1141 cgcggacttg ccccacccgg tcgcctggac gcacagcacc cttcggccct cgcagacgag
     1201 ggcttgcgcg gctaccagct ggtcgggccg aaggaccgcg ctaggcccgg ccaactggtg
     1261 gagcactctg gtggcgacgg cgagcagctg gtctggctcg agtcgagtct gcgtcactcc
     1321 ccgagcatgg cctaccgctc ggacaccccg gcgtggccct ccacaccccg ggcgtggtga
     1381 gccaaatggc ctttagccac ggctgggcgt accgataatc catcgaggct gcccgtcacc
     1441 ctcaaggcca gagatcggcg accgggaaag aggttgtacg tggcgtcccc ggcggaggaa
     1501 acgcctaatc gcggctggac tgcgtggtgg gcctacctca cgctgatttt tatcggctcc
     1561 ggttcgttga tcggcttaag cgcgcgggcg ctaacccacg accacctgca tgggtccgcg
     1621 agcgcgttcg ccctggtcgc ggcgcttctg gtgttgggtg agctgcgccc actggtggcc
     1681 agcgggtcct acgacccgga cggcgtcagc ctgagcactg cgttcgtctt cgcgatcttg
     1741 ctcatctggg gtccggcccc ggccatcatc ttgcagttcg ccggcatctc gatgcgcgag
     1801 gtgctgatgc gcaaggcgtg gtggcgggtg atattcaacg cggggcaata cgcgatctgc
     1861 ctcgcgaccg catcggtcgt gctgcggctg ttcggaatat caccgtcgct agcccatcct
     1921 cacgaggtga ccggcggaac gttgctaggg cttgtcgttg cggcgatcgt ctaccacctg
     1981 atgaacctgc tgctcgtcgg catcattatc tcgattcgcc gcggcaccac cttgtgggcg
     2041 gaaatcatcg acgagttttg gtattacacc tcgacgacga tggcagtgat cgccttgtcg
     2101 ccggtgatcg ccgtggtcgc cgtccatgca tggcaactga tccctctgct gatactgccg
     2161 ctgttccttg tctacaagac cgcgtcgatc tcactcgagc gcgagcagca gtcattgcac
     2221 gacgcgctga cgggtctggc gaaccgcaag ttgctgatgg ctcgcacggg tgagcttctg
     2281 gatgaactcc agctcactga cggccgagtt gcgctggtgt tgttggacct cgaccggttc
     2341 aaggacatca acgacacgct ggggcacccg gtgggagacc gtgtgctgca ggtcgtcgca
     2401 gcacgcgtac aagacgccgt ccgcccaggg gatctggtcg cgaggctagg tggtgacgag
     2461 tttgcgatcc tgcttcatga cctgagcgac gaggcgacgg tgatcgaggt cgccgagcgc
     2521 attcgcgcga cgctgcacga acccatttcc ttcgacggca cgatggtcga cctcgacgcg
     2581 agtctcggca tcgcactttt ccccgatcac ggcgacgatt tcgaccaact gcatcgacgc
     2641 gccgatgtgg cgatgtacgt ggccaagtca tctggcggtg gagttgccgt ctatgacatc
     2701 gagaaggaca ccaactcact caccaggtta ggcctgctcg gtgagctgcg gcacgcggtc
     2761 gaggtcggcg ggttggagct ttggtaccag cccaaggttt cgttggcgac cggcgaggtt
     2821 cttggcgtcg aggccttggt tcgatggcac catccgacgc tcggcctcgt gatgcccgac
     2881 cagttcatcc cgctggccga gcagtccgga ctgatgccgc ggctcaccga cctgctgctc
     2941 gaccaggcgc tcgcggtgcg agcacagctg gacgccggtg ggtcggggct cgtcttggcc
     3001 gtcaacatca gcgtgcgcga cctgctcgat ttcgggttcg ccgacagggt cgccgctgca
     3061 ctgcgccgcc atcacgtcgc acccgaaacc ctcatgctcg agatcaccga gcgccggctc
     3121 ctcgccgact acgaccgtgg cgcgaaggtt ctgagcgaac tcgtcgcgag tggtgtgcat
     3181 atcagccttg acgacttcgg caccggatac tcctcgctga ccatgctgaa gcgggtgccc
     3241 gtctgcgagc tgaagatcga ccagtcgttc gtgtcgagaa ttggcgactc tgaggcagac
     3301 gccaccatcg tcggctcgat cgtcgaactc gcccacgccc tcaacctcac ggtggttgcc
     3361 gagggcgtgg agaccaccga tgtgcttgag cggttgaagg ccctcggctg cgaccaggca
     3421 cagggctggt tggtcggtct tccgatggtc gccgcgacgg ccagcgcatg gatcgcgcgg
     3481 catcgccgtg acacgcaggg cgcccgcgcc ccgttaaggg cactgtccta gcggaaatgc
     3541 agatggccaa agggctatcc gggcgcaata tcgggccttc ccgcgacaag ggaccggtcc
     3601 aggacggata ctgaggggaa tgaacaggac cgcgccgtga tccggcgccc gctgcccggt
     3661 agccccttca acgtgatcac ggtcgcccgg ccggtcgagg tgttcgcggt gaacgaccgg
     3721 gtgagccacg acaggtacgg cctagggtgc gtcatcgggg tcgaacctga cgtcgcggtc
     3781 ttggtcgatt tcggtccacg gcaggagcgc ttggtcgctc cgtacccccg gctgaccaag
     3841 ctttagtgcg aactctcttt gcgaacggtt ctgtctttga cggcaccggc gcgcctgctg
     3901 tgcgcggtgc cgtcgtcatc gagggcgatc gcatcgtcga agtgggtcag ggcaacggca
     3961 acgagatgga cgccgatgtg cgactcgacc tcgcaggtgc ggccgtcgtc ccgggtttct
     4021 tcgactgcca cacccacgtc acggtctccg gctttgacta catgtcattg ctccaacg
//
LOCUS       DATCBJ010000053         4163 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_29537, whole genome shotgun sequence.
ACCESSION   DATCBJ010000053 DATCBJ010000000
VERSION     DATCBJ010000053.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 4163)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4163)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4163
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_29537"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..642
                     /locus_tag="VII50_01205"
     CDS             <1..642
                     /locus_tag="VII50_01205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HEY5049486.1"
                     /translation="HERIDIVGPLGRPFALPREPATCVLVGGGYGTAPLFPLADALRG
                     RGCRVDFVMGAAREERLFGALDAKRISATLTVTTDDGSVGHHGRVSDVLPDVLAASKA
                     DVVYACGPMGMLRAVSQVAAAVGLPCQIAVEELMACGIGVCMTCVLPVIGDDGITRMV
                     RSCVDGPVFHGDRVRFDDVGIIPADVHGAPVPPAAKAPPRTPGPRKARAEVTR"
     gene            639..1625
                     /locus_tag="VII50_01210"
     CDS             639..1625
                     /locus_tag="VII50_01210"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HEY5049487.1"
                     /translation="MTLTVRASTPVTKVASTVDMTTTLGRFALPNPVLTASGCAASGQ
                     ELAQFFDVAELGGVVTKSIMINVRSGRATPRMAETPSGMLNSIGLQGPGIEIFLARDL
                     PWLIEQGARAVVSIAGSTVDEYAKLAMRLREAAGISMIEVNISCPNVEDRGQVFACDA
                     RAAAEVIQAVRRATRSELPVFAKLSPDVTDIVSIARSCVTAGADGLSMTNTLLGMVID
                     TDTLRPVLAGVTGGLSGPAIRPVALRCIWQVHAAMPEVPILGMGGIRTGLDALQFILA
                     GARAVSVGTEIFHDPTAPVRVLAELEEALALRGIMRLLDAVGLAHGERESAP"
     gene            1645..2352
                     /gene="pyrF"
                     /locus_tag="VII50_01215"
     CDS             1645..2352
                     /gene="pyrF"
                     /locus_tag="VII50_01215"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HEY5049488.1"
                     /translation="MTGTAPIAVALDARDLETASAWARAVEPHVSTLKIGLELFCRYG
                     PEAIASVRGGSRIQLFLDLKLNDIPNTVAGAVRSVARLRPAYLTVHALGGRAMIEAAA
                     QELPDGVITAVTILTSLDDGDLAEFGIAGSSQDAVRRLSVISVSAGAGAIVCSAREVA
                     AVRAEVGPSITLITPGVRAPGADAQDQARVATPEQALADGADLLVIGRQITGAADPGA
                     AAASLAAALRRFSRAEK"
     gene            2515..2838
                     /gene="mihF"
                     /locus_tag="VII50_01220"
     CDS             2515..2838
                     /gene="mihF"
                     /locus_tag="VII50_01220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006382163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor, actinobacterial type"
                     /protein_id="HEY5049489.1"
                     /translation="MALPILTPEQRADALAKAAVARQRRAEIKSRLKHSGESLGAVLD
                     LGERDDVVGKMRVTALLESLPGVGKVRAAQIMERLGIASTRRVRGLGTKQRAALVAEF
                     GGGRS"
     gene            2807..3391
                     /gene="gmk"
                     /locus_tag="VII50_01225"
     CDS             2807..3391
                     /gene="gmk"
                     /locus_tag="VII50_01225"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007589232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HEY5049490.1"
                     /translation="MSQSSGADVPEPARLTVLSGPSGVGKGTVVAALARRVPGLWVSV
                     SVTTRFPRPGEIDGVNYRFVDDARFDEMIAAGEFLEYATYGKHRYGTPRKPVEDKLAA
                     GVPALLEIDLQGAQQVRAVMPDVFCVFLTPPSWDELVRRLTGRGTESPEIAAARLERA
                     RLELAAGPEFDAVVVNESVQQACDELVKLLHLRP"
     gene            3415..3678
                     /gene="rpoZ"
                     /locus_tag="VII50_01230"
     CDS             3415..3678
                     /gene="rpoZ"
                     /locus_tag="VII50_01230"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004948662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HEY5049491.1"
                     /translation="MSGIVASPEGITNPPIDDLLEIADSKYALVIYSAKRARQINAYY
                     SQLGEGLLEYVGPLVETHPHEKPLSVALREINGRLLTAEPVEA"
     gene            3693..>4163
                     /locus_tag="VII50_01235"
     CDS             3693..>4163
                     /locus_tag="VII50_01235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavoprotein"
                     /protein_id="HEY5049492.1"
                     /translation="MTAAHSPRESGRDADRPRIVLGVTGGIAAYKACELLRLLTEANF
                     DVTVVPTQAALKFVGAPTWAALSGNPVRVDLFDDVHEVAHVRLGQQADLVLVVPATAD
                     VLARAAHGLADDLLTNVLLTSRGPVVFAPAMHTEMWDHPATAANVATLRSRGAIV"
BASE COUNT          622 a         1351 c         1436 g          754 t
ORIGIN      
        1 cacgagcgga tcgacattgt cggacccctt ggccggccct tcgcgctgcc gcgggagccg
       61 gcaacttgcg tgctcgttgg cggcggctac ggcacggcac cgctcttccc gctcgccgac
      121 gcgttgcgcg gtcgggggtg ccgcgtcgac ttcgtgatgg gcgcagccag ggaggagcga
      181 ctgtttggcg cacttgacgc gaaacggatc tccgcgacgc tgacggtcac gacggatgac
      241 gggtcggtag gccaccacgg ccgagtatcc gacgtcctgc ccgatgttct tgcggcaagc
      301 aaggcggacg tcgtgtacgc ctgcggcccc atgggcatgc tgcgagccgt tagccaggtc
      361 gctgcagcgg tcggcctgcc ttgtcagatc gccgtcgagg agttgatggc ctgcggcata
      421 ggcgtctgca tgacctgcgt cctgccggtg atcggtgacg acgggattac ccggatggtc
      481 cgctcgtgcg tcgacggtcc ggtgttccac ggtgatcggg tgcgcttcga cgacgtcggg
      541 atcatcccgg ctgacgtgca tggtgcgccg gtgccacccg ccgcgaaagc gccgccgcgc
      601 accccgggcc cgcgcaaggc ccgcgccgag gtgactcgat gacgctgacg gtccgcgcct
      661 cgacgccggt gaccaaggtc gcgagcaccg tcgacatgac aacgacgctg ggcaggtttg
      721 cgctacccaa cccggtgctg acggcgtccg gttgcgcggc atcgggacag gaactcgcgc
      781 agttcttcga tgtcgccgaa cttggtggcg tcgtcacgaa gtccatcatg atcaacgtcc
      841 ggtccggtcg cgcgactccg cgaatggccg agacgcccag tgggatgttg aactcgatcg
      901 ggctgcaggg gcccgggatc gagatcttcc ttgcgcgcga tcttccatgg ttgatcgagc
      961 agggtgcgcg cgcggtggtg tcgatcgccg gcagcaccgt cgatgagtat gcgaagttgg
     1021 cgatgcggtt acgcgaagcg gctgggattt cgatgatcga ggtcaacatc tcgtgtccga
     1081 acgtcgaaga ccgtggccaa gtgtttgcgt gcgacgccag agcggccgcc gaagtgatac
     1141 aggcggtgcg ccgcgctacg cgctcagaac tcccggtatt cgccaagctg tcaccagacg
     1201 tcaccgacat cgtgtcgatc gcccgctcgt gcgtgactgc tggagcagac ggattgtcga
     1261 tgacgaacac gttgctcggc atggtgatcg acaccgacac cttgcgcccg gtgctcgccg
     1321 gagtcaccgg tgggttgtct ggtccggcga ttcgcccagt tgccctgcgg tgcatctggc
     1381 aggttcacgc tgcgatgccc gaggtgccaa tccttggcat gggcggtatc cgcaccggcc
     1441 ttgacgcgtt gcagttcatc ctcgctggcg cgcgtgccgt atctgttggc accgagatct
     1501 tccacgatcc gaccgcgccg gtgcgtgtgc ttgccgagct cgaggaggcc ctggcgctgc
     1561 gcggcatcat gcgattgctg gacgccgtgg ggctggcgca cggtgaacgc gagagcgcgc
     1621 catgagcgaa cgcgcgagcg cgccatgacc gggaccgcac ccatcgcggt cgcgctcgat
     1681 gcgcgcgacc ttgagacggc aagcgcgtgg gcacgtgcgg tcgagcctca tgtgagcacc
     1741 ctgaagattg gcctcgaact cttctgccgt tacggtcccg aggcaatcgc cagcgtgcgc
     1801 gggggcagcc gcatccagct gttcctcgac ctgaagttga acgacatccc caacacggtg
     1861 gcgggtgcgg ttcgctctgt cgcgcgactt cgcccggcct acctcaccgt gcatgcgctc
     1921 ggcggccgcg cgatgatcga agccgcagcg caggaattgc ccgatggcgt catcacggcg
     1981 gtcacgattt tgaccagcct cgacgacggc gacctcgccg agttcggaat cgccggcagt
     2041 tcgcaggacg ccgtgcgcag gctttcggtc atctcggtct ccgccggtgc cggcgccatc
     2101 gtttgctcag cgcgcgaggt agccgccgtg cgggcggagg ttggcccgag tatcacgctg
     2161 attacgccgg gagtgcgggc gcctggtgcg gacgcacagg atcaggcgcg agtggccaca
     2221 cctgagcagg cgctcgccga tggtgccgat ctgttggtga tcggccggca gatcacgggt
     2281 gctgccgacc ccggtgcggc cgcagcctca ctcgctgctg cattgcgccg attctcgcgc
     2341 gccgagaaat agtcgtcgtc ctcgcgccgc tcgaattgga ggcttgagta agggccgcgt
     2401 tgcccgagac cgctcgttgt cgctaggttg cgaactcgtc ggggcaggaa caccgcgtcg
     2461 acgcccattc ggtccactcc ggcctcgcgc cgccgttcgt tcgaggtgac cttcgtggca
     2521 ctcccgatcc tgacgcccga gcagcgagcc gatgcacttg cgaaagctgc ggtcgcgcgg
     2581 cagcggcgcg ctgagatcaa gtcaaggctc aaacattccg gagagtcgct tggcgccgtg
     2641 ctcgaccttg gagaacgcga cgacgtggtc ggaaagatgc gggtgaccgc gctgctcgaa
     2701 tcattgccgg gcgtcgggaa ggtacgagcg gctcagatca tggagcggct cggcattgca
     2761 tcgaccaggc gggtgcgagg tctcggtacc aagcaacgtg ccgcgcttgt cgcagagttc
     2821 gggggcggac gttcctgagc cggctcgcct gacggtgctg tccggtccct ctggtgtcgg
     2881 caagggcacc gtcgtggccg cgctcgcgcg tcgtgtccct gggctttggg tctcggtatc
     2941 ggtcaccaca cgttttccgc ggcccggtga gatcgacggg gttaactatc gcttcgtgga
     3001 cgacgcgcgg ttcgacgaga tgatcgccgc cggtgaattc ctcgagtacg cgacatacgg
     3061 caaacaccgg tacgggacgc ctcgcaagcc cgttgaggac aagttggccg ccggcgtccc
     3121 tgcgttgctg gagatcgacc tgcagggggc gcagcaggtg cgggcggtga tgccggacgt
     3181 cttttgcgtg ttcttgacgc cgccctcgtg ggacgaactc gtgcgccggc tcaccggccg
     3241 gggtaccgaa tcgcctgaga ttgccgccgc ccgcctcgag agggcccggc tggagcttgc
     3301 tgctggccct gagttcgacg cggtcgtggt caacgagtcc gtccagcagg catgtgacga
     3361 actggtaaag ttgttgcatc tgcgcccgta ggcgccttaa ccgaagggca tcgcgtgtca
     3421 ggcattgtcg ccagtcccga gggcatcacg aatccgccta tcgatgactt gctcgagatc
     3481 gccgactcca agtacgccct ggtcatctac tcggccaagc gcgcgcggca gatcaacgcg
     3541 tactactccc agctcggtga gggtctgctc gagtacgtcg ggccgctcgt cgagacgcac
     3601 ccccacgaga agccgctgtc cgtcgccttg cgcgaaatca acggccggct gctgaccgcc
     3661 gagccggtcg aggcctgacg ccggcgggtg tgatgactgc cgcacactcg cctcgcgagt
     3721 ccggccgcga cgcggaccgg ccgcgcatcg tgctcggggt taccggtggg atcgcggcct
     3781 acaaggcctg cgaactgttg cggctgctga ccgaggcgaa ctttgacgtg acggtcgttc
     3841 cgacgcaggc cgcactgaag tttgtcggtg cgccgacgtg ggccgcgctg tccggcaatc
     3901 cggttcgcgt cgatcttttt gatgacgtgc acgaagtcgc gcacgtgcgc cttggccaac
     3961 aggccgactt ggtgctcgtc gttccggcca ctgccgacgt gctggcccgc gcagctcacg
     4021 gcctcgccga cgacttgctc acgaatgtgt tgctgacgtc gcgcggccct gtcgtgtttg
     4081 ccccggcgat gcataccgag atgtgggacc accccgccac cgcggccaac gtggctacct
     4141 tgcgatcccg cggcgccatc gtg
//
LOCUS       DATCBJ010000054         2847 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_67331, whole genome shotgun sequence.
ACCESSION   DATCBJ010000054 DATCBJ010000000
VERSION     DATCBJ010000054.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2847)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2847)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2847
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_67331"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            329..640
                     /gene="groES"
                     /locus_tag="VII50_01240"
     CDS             329..640
                     /gene="groES"
                     /locus_tag="VII50_01240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HEY5049493.1"
                     /translation="MTTATKSKVAIRPLEDRIVVQAVEAETTTASGLVIPDTAKEKPQ
                     EGIVLAIGPGRFEDGQRVPLDVKEGDRVLYSKYGGTEVKYSGEEYLVLSARDVLAVIE
                     K"
     gene            759..2393
                     /gene="groL"
                     /locus_tag="VII50_01245"
     CDS             759..2393
                     /gene="groL"
                     /locus_tag="VII50_01245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020643281.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HEY5049494.1"
                     /translation="MPKILQYDETARRSLERGVDALANAVKVTLGPKGRNVVIAKSYG
                     VPTITNDGVTIAREIELLDRHENLGAQLAKEVATKTNDVAGDGTTTATVLAQAMVHEG
                     LRNVAAGADPLALKRGMEAAVTAVTARLLDTAREVSGKGEIAHVAAISAQDPEVGGLI
                     AEAFDKVGKDGVITVEESNTMGLELELTEGLQFDKGYISPYFVSDTERMEAVLENPLI
                     LLNQGKLSSVSDLLPLLEKVIQAGKSLLVIAEDVDGEALSTLVVNKIRGTFSSVAVKA
                     PAFGDRRKAMLEDIAILTGAQVVAPEVGLKLDDVGLEVLGTARRVTITKDDTTIVDGA
                     GSAEAVADRVRQLRAEIDRTDSEWDRDKLQERLAKLAGGVAVIRVGAATEVELKEKKH
                     RLEDAISATRAAVEEGIVAGGGSALVHAAAALDKLDLVGDEATGATIVRKALVEPLRW
                     IAENSGLEGYVVTQKVAGLPIGHGLNAATGDYGDLIADGVIDPVKVTRSALANAASIA
                     ALVLTTETLVVEKPEDTEADAHGGHGHSHGPGGHSH"
     gene            2403..>2847
                     /locus_tag="VII50_01250"
     CDS             2403..>2847
                     /locus_tag="VII50_01250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5049495.1"
                     /translation="MDLDEAAPTAQRGPGAPDALAALLAKGERAAFHGPPGTAVPALE
                     EAVALATRSGRLAEATAATWLLGVAQASRGRFGTALTLLGGVMGPLRDALSGADTAPE
                     LRLFGALAAATVASVHRQLGRHTVAWEFDALGLTAADALPGLDEAV"
BASE COUNT          494 a          972 c          951 g          430 t
ORIGIN      
        1 gccgttgcgt gggcgatggc ttcgcgccca ggctccgtcg cgagcgcccc gaacgtgtcg
       61 gcgtccatcg agcaaccctc ctcgccgggt ccccggcgaa agcgctggcg ggagcgcatt
      121 catgcctggc actcgggttg acggagtgct aagcccggca tagtcttcga gtggacgtgc
      181 tggcactctc gtaccccgtt tgctaacacg ttgcttgctc ggtctgaccc ccgcgacggc
      241 aggccggtgt ggacaccaac tgctccagat caccaagtgc taacgcccaa ccatcatcac
      301 aacaacactg aggaggggga gtcacgctgt gacgaccgcc accaagtcca aggtagccat
      361 ccggccgctc gaggaccgca tcgtcgtgca ggccgttgag gccgagacca ccaccgcttc
      421 cggcctagtc atccctgaca ccgccaagga gaagccccag gagggcatcg tcctcgccat
      481 cggccccggc cggttcgaag acggtcagcg ggtgcccctc gacgtcaaag agggtgaccg
      541 cgttctctac agcaagtacg gcggcactga ggtcaagtac agcggcgagg agtacctcgt
      601 actctccgct cgcgacgtcc tcgccgtcat cgagaagtag cttgctcgcg gcgtaagtcg
      661 caccggctcg cgccgccgcc ccggagccac acggccccgg ggcggcgtcc gtcgggcccc
      721 agcttgaacc agtgaattgt tgagaggacc tctaccgcat gccgaagatc ctgcagtacg
      781 acgagaccgc ccgccgatcg cttgagcgcg gtgttgacgc gctcgcgaac gccgtgaagg
      841 tgacgcttgg gcccaagggc cgcaacgtcg tcatcgccaa aagctacggc gtcccgacga
      901 tcaccaacga cggcgtgacc atcgctcgcg agatcgagct ccttgaccgc cacgaaaacc
      961 tcggtgcgca gctcgccaaa gaggtggcca ccaagaccaa cgatgtggct ggcgatggca
     1021 cgacgaccgc cacggtgctc gcgcaggcga tggttcacga aggcttgcgc aacgtcgcag
     1081 ccggcgccga cccgctcgcg ctgaagcgtg gcatggaagc cgccgtcacc gccgtgaccg
     1141 cacgtctcct cgacactgcg cgtgaggtat ccggcaaggg tgagatcgct cacgtcgccg
     1201 cgatctcggc gcaagacccc gaggtcggcg gcctcatcgc cgaagcgttc gacaaggtcg
     1261 gcaaggacgg cgtcatcacc gtcgaagagt cgaacacgat gggcctcgag cttgagctca
     1321 cggagggcct gcagttcgac aagggctaca tctcgccgta cttcgtcagc gacaccgagc
     1381 gcatggaagc ggtcctcgaa aacccgctga tcctgctcaa ccagggcaag ttgtcatcgg
     1441 tgtccgacct gctgccattg ctggaaaagg tcatccaggc cggcaagtcg ttgctggtca
     1501 tcgccgagga cgtcgacggc gaggccctct cgactctggt cgtcaacaag attcgcggca
     1561 ccttctcctc ggtcgcggtc aaggcacccg cgttcggcga tcgccgcaag gccatgctcg
     1621 aagacatcgc gatcctcacc ggtgcgcagg tcgtggcgcc cgaggtcggc ctcaagctgg
     1681 acgacgtggg gctggaagtc ctcggcactg cccgccgggt caccatcacg aaggacgaca
     1741 cgacgatcgt tgacggcgcc ggctcggccg aggcagtcgc cgaccgcgtt cgccagttgc
     1801 gcgcggagat cgatcgcact gactccgagt gggatcgcga caagttgcag gagcggctgg
     1861 cgaaacttgc cggcggcgtc gcggtgatcc gggtcggtgc cgcgaccgaa gtcgagctca
     1921 aggagaagaa gcaccggctc gaggacgcga tctcggccac ccgcgcggcc gtcgaagagg
     1981 gcatcgtggc cggtggcggc tccgctttgg tacacgcggc ggcggcgctc gacaagctcg
     2041 acctcgtcgg tgacgaggcg accggcgcga ccatcgtgcg caaggcgctc gtcgaaccgc
     2101 tccggtggat cgccgagaac agcggtctcg agggctacgt cgtcacgcag aaggtcgccg
     2161 gattgccgat cggccatggg ctcaacgccg cgaccggcga ctacggcgat ctgatcgctg
     2221 acggagtcat cgacccggtc aaggtgacgc gatctgcgct ggcgaacgca gcctctatcg
     2281 cggcgctggt gctcacgacg gagacactgg tcgtcgagaa gcctgaagac accgaggccg
     2341 acgcgcacgg tggtcacggt cacagccacg gtcccggcgg tcacagccac taacctggcg
     2401 cggtggacct tgacgaggct gccccaacag cccagcgcgg ccccggcgcg ccggacgctc
     2461 tcgctgcgct gctagcgaag ggcgagcgcg cggcgtttca cggaccgccc ggcaccgccg
     2521 tccccgctct cgaagaggct gttgcactcg cgacccgtag cggtcggctg gccgaagcga
     2581 ccgcggcgac ctggctcctc ggcgttgcgc aagcatctcg tggtcggttc ggtaccgcac
     2641 tgactctgct tggcggagtg atgggtccgc tgcgcgacgc cctctcgggc gcggacaccg
     2701 ctcctgagct gcggctgttc ggtgcgctcg cggcagcgac agtcgccagc gtgcaccggc
     2761 agctcgggcg gcacaccgtg gcgtgggagt tcgacgcact cgggctgacg gctgcggatg
     2821 cgctgccggg gctcgacgaa gccgtct
//
LOCUS       DATCBJ010000055         2938 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_360882, whole genome shotgun sequence.
ACCESSION   DATCBJ010000055 DATCBJ010000000
VERSION     DATCBJ010000055.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2938)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2938)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2938
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_360882"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..957
                     /locus_tag="VII50_01255"
     CDS             <1..957
                     /locus_tag="VII50_01255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009073117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEY5049496.1"
                     /translation="GAEAVENAVKIARHATGRPAIIAFDHAYHGRTNLTMALTAKNMP
                     YKQGFGPFAGEIYRVPMAYPYRWPSGPENCGPEAAVEVIGAMKTQLGVDSIAAVLIEP
                     IQGEGGFIVPGAGFLTALSEFCTANGIVFIADEIQTGFARTGEMFACTYEGVVPDLIT
                     TAKGIAGGMPLSAVTGRTEIMDAAHVGGLGGTYGGNPVACAAALGAMRTIDELDLVAR
                     ARDIEAVMLPRLRAMAEKFDCIGDVRGRGAMIAVEIVKPGTIEPDAATTSAVAKACHA
                     AGVVVLTAGTYSNVLRFLPPLVIPEHLLHEGLDVVEQAFAAL"
     gene            complement(1037..1378)
                     /locus_tag="VII50_01260"
     CDS             complement(1037..1378)
                     /locus_tag="VII50_01260"
                     /inference="COORDINATES: protein motif:HMM:NF014677.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF190 domain-containing protein"
                     /protein_id="HEY5049497.1"
                     /translation="MTVDGLVARLTIYLGAADRTGKRLAPEDVVRRAHTAGLTGATVS
                     RASQLDGDTNRPRTISLSHPAAVTVTILGTEPILRTFLAGLDDLREAGATMLLEGADE
                     VHEANTQLAGP"
     gene            complement(1516..>2938)
                     /locus_tag="VII50_01265"
     CDS             complement(1516..>2938)
                     /locus_tag="VII50_01265"
                     /EC_number="2.7.7.65"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA];
                     GO_function: GO:0071111 - cyclic-guanylate-specific
                     phosphodiesterase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional diguanylate
                     cyclase/phosphodiesterase"
                     /protein_id="HEY5049498.1"
                     /translation="HLAAVVACDLTVLWALGDADYGQVVVMPGALIVVAVVVGRLVRA
                     ADAAEIDTLTGLPNRRGLDRHLGEALAGGSPGIHSVALAFLDVDHFRSVNNTEGQGGG
                     DRLLRAIAAAWRPLLRDTDFLARNGSDEFALLITDALPGEVAMVIGRLQAAMPKAHTC
                     SAGASDWQRGDTVSLLAARTDTALYESKRNGRNRLTYHDSSGNSEEAQLRRAVADGEI
                     VAYYQPIVELGSGVVTGFEALARWIRPGHGLVVPDAFIPVAEENGAIEDIGSMMLAAA
                     ARQVALWSIAQSRPLTASVNVCGRELRNPGYVAQVKEILRDAGLPAAQLVLELTETAF
                     EGNAAPVIAALDELHALGIGIAIDDFGTGYSSLSRLDRLPVDVVKIDRSFVSTLPAGA
                     TRAPLVAAIIAMAQALGLHVVAEGIEHPHQVALLFGLGCNKGQGYWFDRPCPADEVDL
                     SPREPAFVTSVGTGSAAALRYAG"
BASE COUNT          539 a          967 c          957 g          475 t
ORIGIN      
        1 ggggcggaag cggtggagaa cgccgtcaag atcgccaggc atgccaccgg ccggcccgcg
       61 atcatcgcct tcgaccacgc ctaccacggc cggaccaacc tcacgatggc gctgacggcc
      121 aagaacatgc cttataaaca aggctttggc ccgttcgccg gcgagatcta ccgcgtaccg
      181 atggcctacc cctaccgctg gccgagcggg ccagagaact gcggccccga agcggcggtg
      241 gaggtcatcg gggcgatgaa gacgcagctc ggcgttgaca gcatcgccgc cgttctcatc
      301 gagccgatcc agggcgaggg cggtttcatc gttcccggcg cgggcttcct cacggcgttg
      361 tccgagttct gcaccgcgaa cggcatcgtc ttcatcgccg acgaaattca gaccggcttc
      421 gcccgtaccg gcgagatgtt cgcgtgtact tacgagggcg tcgtccccga tctgatcacg
      481 acagccaagg gcatcgctgg cggaatgccg ctatccgcag ttaccggccg gaccgagatc
      541 atggacgccg cacacgttgg cggcctgggt ggcacgtacg gcggcaaccc ggtggcttgc
      601 gctgcggcgc tgggcgcgat gcgcacaatc gacgagctcg atctggtggc acgcgcgcgc
      661 gacatcgaag cggtcatgct gccccgactt cgtgcgatgg cggagaagtt cgactgcatc
      721 ggcgacgtcc gcgggcgggg cgcgatgatc gccgtcgaga tcgtgaagcc gggcacgatc
      781 gagcccgacg cagccacgac atcggcggtg gcgaaggcct gtcacgccgc tggagtcgtt
      841 gtgctgacag caggcacgta cagcaacgtc ttgcgattcc tgccaccgct ggtgattccg
      901 gaacacctgc tgcacgaagg tctcgacgtc gtagaacagg ccttcgccgc cctgtaataa
      961 gaatcgtggg cgtttaccgc ctctgagggt agtaaagacc cacgatctca tcgggagact
     1021 gaaagaacgg ccgctattac gggccggcga gctgggtgtt ggcctcgtgc acctcgtcgg
     1081 caccttcgag cagcatggtc gcgccggctt cgcgcaaatc gtcgaggccg gcgaggaatg
     1141 tgcgcaagat cggctcggtg ccaagaatcg tgacggtcac cgcagcaggg tgcgacaacg
     1201 agatcgttcg cggacggttg gtgtcgccgt ccagctgcga ggcgcgcgaa acggtcgcgc
     1261 cagtcagccc ggccgtgtgc gcgcgccgaa cgacgtcttc tggcgcaagt ctcttcccgg
     1321 tgcggtcggc tgcgccgaga tagatggtga gccgagccac caaaccgtct accgtcatcg
     1381 ggcactcctc ggatcattgc ggcaagtggt gcgccgcggt ccatcgtgcc aactggactc
     1441 cgaaatgtgc gactgccgag ccgataaaca tgtgggtagt taggcagaat gccccttatg
     1501 ggctattaga tcctgtcagc cagcgtagcg cagtgctgcc gccgagcccg tacccacgct
     1561 cgtgacaaac gccggctccc gcggcgatag atcaacctcg tctgccgggc atggccgatc
     1621 gaaccagtac ccctggcctt tgttgcaacc caggccaaac agcaacgcaa cctgatgcgg
     1681 gtgctcgatt ccctcggcaa ccacatggag cccaagagcc tgcgccatgg cgatgatggc
     1741 ggccaccaac ggtgcccggg tcgccccggc gggcaacgtg ctcacgaaag atcggtcgat
     1801 cttcacgacg tcgaccggca gccggtcaag ccggctcagg ctggagtacc cggtaccgaa
     1861 atcgtcgatc gcgatcccga tgccgagcgc gtgcagttcg tcgagcgccg cgatcaccgg
     1921 cgcggcattg ccctcgaacg ccgtctcagt gagctcaagc accagctgag ccgccggcaa
     1981 cccggcgtcc cgcagaattt ctttcacctg cgccacgtag cccggattgc gcaactcccg
     2041 cccacagacg ttgacgctgg cggtaagcgg cctggactgg gcgatggacc agagggcaac
     2101 ttggcgcgcg gccgcggcga gcatcatgga gccgatgtcc tcaatggcgc cgttttcctc
     2161 ggcgacgggg atgaacgcgt cgggtaccac cagaccgtgg ccgggtcgga tccaccgcgc
     2221 gagagcttcg aagccggtca cgacaccact gccgagctcg acgatcggtt ggtagtacgc
     2281 aacgatctcg ccgtcagcaa cggcgcgacg aagctgcgcc tcctcggaat tcccgctgct
     2341 gtcgtggtac gtaagcctgt tgcggccgtt gcgctttgac tcatagagcg cagtgtcggt
     2401 gcgcgcagcg agcagcgaca ccgtgtcgcc acgctgccag tcggatgcac cggcagaaca
     2461 ggtgtgcgcc ttcggcattg ccgcctgcaa ccgaccgatc accatcgcga cttcgcccgg
     2521 caatgcgtca gtgatcaaca gggcgaattc atcgctgccg ttgcgcgcaa gaaagtcggt
     2581 gtcacgcagc agtggtcgcc acgcggccgc aatagctcgc aggagccggt cgccgccgcc
     2641 ctgaccttcg gtgttgttga ccgatcggaa atggtcgacg tcgaggaacg ccagggctac
     2701 ggaatggatg ccaggcgatc cgccggcgag agcttcgccc aagtgccggt cgagtccgcg
     2761 gcggttcggc agtcccgtca acgtatcgat ctcggccgca tcggcggcac ggaccagtcg
     2821 gccaacgacg accgcgacga cgatcaatgc acccggcatc acgacgacct ggccgtagtc
     2881 ggcgtcgccc aatgcccaga ggaccgtcaa gtcgcacgcg acgacggcgg cgagatgc
//
LOCUS       DATCBJ010000056         2970 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_177629, whole genome shotgun sequence.
ACCESSION   DATCBJ010000056 DATCBJ010000000
VERSION     DATCBJ010000056.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2970)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2970)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2970
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_177629"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..191)
                     /locus_tag="VII50_01270"
     CDS             complement(<1..191)
                     /locus_tag="VII50_01270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5049499.1"
                     /translation="MNKTAIRLVAVAAVAGLGIAGCSSKKASTAASSAPAATTSSAAP
                     AATSSAAPAATSSAAPPAS"
     gene            complement(384..1268)
                     /locus_tag="VII50_01275"
     CDS             complement(384..1268)
                     /locus_tag="VII50_01275"
                     /inference="COORDINATES: protein motif:HMM:NF014665.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0016653 - oxidoreductase activity, acting
                     on NAD(P)H, heme protein as acceptor [Evidence IEA];
                     GO_process: GO:0006784 - heme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="COX15/CtaA family protein"
                     /protein_id="HEY5049500.1"
                     /translation="MALTSLIANVGIVSTGGLVRLTNSGLGCPSWPDCSSGDLVPVRA
                     LSSHKAIEFGNRALTTLLVIAVAAVFVAVWRSAPRHAGLVRWAWVTLLGVPAQIVVGG
                     VLIFTHLNPIAVAGHFMLSMALIAAATVLWWRTREPADIESASPPAVPVVVSRLGWAT
                     WLLTYAVFIVGTLVTGSGPHAGDATAKRLHLQPKAISQLHADLVMLLVGIAVALAVCV
                     TAVGADAVARRTTRVLIGVLLAQATVGFAQYFSGLPVPLVELHVTGAAVLAATATAVV
                     LSLRGERAQNVQARQKAL"
     gene            1472..2233
                     /locus_tag="VII50_01280"
     CDS             1472..2233
                     /locus_tag="VII50_01280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEY5049501.1"
                     /translation="MKSTMVSEVPTAPGVVGGPDDARTRDRVARALHDHGPQSAGALS
                     ASFGLTPAAVRRHLDALIAAGRVCEVPERKVHGQLRGRGRPAKLFALTDNGHSLFPHA
                     YDDIAVDALNYLRDAGGDAAVGAFARQRLQGLEIRYAAALAQVPADRRPAELAAALTA
                     DGYAASAQQAGTGIQVCQHHCPIAHVAADFPQLCEAETQMFVRLLGTHVQRLATIAHG
                     DGICTTHIPLAPAGSSTPVVGSIPSPVQQPGEVTA"
     gene            2230..>2970
                     /gene="sufB"
                     /locus_tag="VII50_01285"
     CDS             2230..>2970
                     /gene="sufB"
                     /locus_tag="VII50_01285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019633769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HEY5049502.1"
                     /translation="MTTIEHPELDQLGRYKFGWSDSDVAGAAARRGLSEDVVRDISAK
                     KSEPAWMLEQRLKGLRIFEKKPMPLWGADLSGIDFDNIKYFVRSTEKQADSWDDLPAD
                     IRNTYDKLGIPEAEKQRLISGVAAQYESEVVYHKIREDLEAQGVLFLDTDTALKEHPE
                     MFQEYFGTVIPVGDNKFSALNTAVWSGGSFIYVPPGIHVDIPLQAYFRINTENMGQFE
                     RTLIVVDEGSYIHYVEGCTAPVYSSDSLH"
BASE COUNT          528 a          969 c          993 g          480 t
ORIGIN      
        1 cttgacgcgg gaggggccgc cgagctggtg gcagcaggcg ctgctgagct ggtggcggcg
       61 ggcgctgcgg agctggtggt ggcggcggga gcgctggacg ccgccgtgga tgccttcttg
      121 ctgctacagc cggcaatgcc gagcccggct accgccgcga cggcaacgag ccgaatcgca
      181 gtcttgttca tgcacggtcc cctttttgct cctgggcgca ggacgtggtg ccctgcagga
      241 ctgcccagtt gttgtgccgc acaacatatg caccgaggac cggtgaacgt aagggccaca
      301 gagatcatct ttgcgttcgg aatggatctg ttacatacgc gtgaccctgc gggtacacac
      361 ccgacgggca tctttatgcc gttttacaac gctttctgcc gagcctggac gttctgggca
      421 cgctcgccgc gcagggacag gacgacggcc gttgcggtgg ccgccagaac agccgcgccg
      481 gtcacatgca actcgaccaa tggcaccggc agcccgctga aatactgcgc gaacccgact
      541 gtcgcctgtg ccagcagcac gccgatgagc actcgcgtcg tccgtcgggc aacagcgtca
      601 gcgccgaccg cggtgacaca caccgcgagc gcgactgcga tgccaaccag cagcatcacg
      661 aggtcggcat gcagctgact gatcgccttc ggctgcaggt gcagccgctt ggctgtcgca
      721 tcgcccgcgt gtggaccgct gcccgtcacg agcgtgccga cgatgaacac ggcatatgtc
      781 agcaaccagg tggcccagcc caggcgactc acgacgacgg ggacggcggg cggcgacgcc
      841 gattcgatgt ccgcaggctc gcgggtacgc caccagagca cggtggcggc cgcgatcagc
      901 gccatcgaca acatgaagtg cccggcgacg gcgatcggat tcaggtgcgt gaagatcaac
      961 accccaccga ccacgatctg cgcggggaca ccgagcagcg tgacccaggc ccagcgaacg
     1021 aggccagcat ggcgcggggc ggaacgccag acggcgacga aaacggccgc aacggcgatc
     1081 acgagcaggg tcgtgagcgc gcggttgccg aactcgatgg ctttatgaga ggacagcgcg
     1141 cggaccggga cgaggtcgcc gctcgaacag tccggccacg acgggcagcc caggcccgag
     1201 ttggtcagcc ggaccaagcc gccggtcgag acgatgccca catttgcgat cagcgatgtc
     1261 agtgccaacc gccgcactgt cgatggagct ggccgccgga ttatggcaac cgcgattgac
     1321 cgccgggggg cgggtggggc tgcgggtcgg gcggtggatg ctgtcatcgc ggtcgatggt
     1381 aggccgcctg tgcaggctcc aaccaggccg ggccgaaccc aggcaggagg gggtgtgtgc
     1441 aggtcggggg taaataggta acaatggtgt tgtgaaatcc acgatggtct ctgaggtgcc
     1501 gaccgctcca ggcgttgtcg gtggcccgga cgacgcccgc acccgcgacc gagtcgcccg
     1561 ggcgttgcat gatcatggtc cccagagcgc aggcgcgctg agcgcgagct ttggcctcac
     1621 gcccgcggcg gttcgccgcc atctcgacgc gctgattgcc gccgggcgag tgtgtgaggt
     1681 tcccgagcgc aaggttcatg gtcagctccg tggccgtggc cggccggcca agttgttcgc
     1741 cttgaccgac aacggtcatt cgttatttcc ccatgcctac gacgacattg ccgtcgatgc
     1801 cctcaactac ttgcgggacg ccggtggcga tgctgccgtg ggcgcgtttg cgcgccaacg
     1861 cctgcagggt ctcgaaatcc gctacgccgc cgcattggcg caagtcccag ccgaccggcg
     1921 tccggctgag ttggctgcgg cgctcacggc cgacggctac gcggcgtccg cgcagcaggc
     1981 cggcaccggc atccaggtct gtcagcacca ctgcccgatc gcgcacgtag ccgcagactt
     2041 cccgcagctg tgcgaggccg agacgcagat gtttgtccgg ctcctgggta cccacgtcca
     2101 gcggctcgcc accatcgctc atggcgacgg catatgtacg acccacattc cgttggcgcc
     2161 tgcaggcagt tcaacgcctg tcgtcggttc aatcccgtcc ccagttcaac agcccggaga
     2221 ggtcaccgca tgacgaccat cgagcatcca gagctcgacc agctcggccg gtacaagttc
     2281 ggctggtccg actccgacgt ggcaggggcg gcggcgcggc gtggcctttc cgaggacgtc
     2341 gttcgcgaca tctccgcaaa gaagagcgag cccgcgtgga tgctcgagca gcggttgaag
     2401 ggcctgcgca tcttcgagaa gaagccgatg ccgctgtggg gcgccgacct gtcggggatc
     2461 gacttcgaca acatcaagta cttcgtgcgc tcgacggaga agcaggccga cagctgggac
     2521 gacctgcctg ctgacatccg caacacctac gacaagctcg gcatccccga ggccgagaag
     2581 cagcgcctga tctctggtgt ggccgcgcaa tacgagtctg aagtcgttta ccacaagatt
     2641 cgcgaagacc ttgaggccca gggtgtgctg ttcctcgaca ccgacaccgc gctgaaggag
     2701 cacccggaga tgttccagga gtacttcggc accgtcatcc cggtcggcga caacaagttc
     2761 tccgcgctga acaccgcggt gtggtcgggt ggctcgttca tctatgtgcc gccgggcatc
     2821 cacgtcgaca tcccgttgca ggcatacttc cgcatcaaca ccgagaacat gggccagttc
     2881 gagcgcaccc tgatcgtcgt cgacgaaggt tcgtacatcc actacgtcga gggttgcacg
     2941 gcgccggtgt actcgagtga ctcgttgcac
//
LOCUS       DATCBJ010000057         3245 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_508671, whole genome shotgun sequence.
ACCESSION   DATCBJ010000057 DATCBJ010000000
VERSION     DATCBJ010000057.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 3245)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3245)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3245
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_508671"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1051)
                     /locus_tag="VII50_01290"
     CDS             complement(<1..1051)
                     /locus_tag="VII50_01290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5049503.1"
                     /translation="MEHTLSSRRFPAAVGVVVLATGVFAAWVGLRVGGGQVTLLVDDL
                     ATPLAALIGCGLCFRARARHDGRMRLFWLLLGLAMACWTVAEVIWGYYALALDAAVPV
                     PSWADIGYLSAIPLAVAALVVHPATHGSGTRKTRSLFDGLVVAAALLFLSWTLVLGSL
                     WRSTNLDTWSGIVAVAYPFGDVVIVFFIVLAMRGMTSGDRLSLWCLLAGLLAMAVSDS
                     TFTYLTASPSYASPGLIDTGWVAAYLGIALAAFSSQPSSGRVLRAERARPSLASLISP
                     LVPVLLALAVAAVQIRLGHQLDHAAWVMAFGLVVLVLVRQGLILFELFAHSRDTQAGL
                     MERLTHAALGGAGESQ"
     gene            complement(1488..>3245)
                     /locus_tag="VII50_01295"
     CDS             complement(1488..>3245)
                     /locus_tag="VII50_01295"
                     /inference="COORDINATES: protein motif:HMM:NF013799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HEY5049504.1"
                     /translation="AAIAAVAIGGTVAISAPSASAHHKGQIKHAVLISVDGMHQSDLD
                     WYIANNPSSELAQLASGGAEFTNNHTSDPSDSDPGGTALMTGGDPRATGVYYDVEYSH
                     AVDEAGAACTPGQPATGGDVIYDSPDDTLNAVPDLLNNGSGNTFPSFDEGGSIYHNGV
                     DTKPAAIMNLSAHPQSGLNTGSFPVDPTTCQPIMPWDYLKVNTIFQVIHSAGLRTAWS
                     DKHAIYTSFNGPGSGGTSIDDFFGPEIDSQAVEPNGVPYPTDTDWAHDDAATKQYDGY
                     KVQAVINELNGYDHSGTQKVGVPAILGMNFQTVSVAEKVDSPSTLTENANGSYTEGPT
                     ELAGYLPGGTTPGPLLASAFDYVNAQLQQMADTIQQDGLASTTAIIITAKHGQSPQDP
                     LQLKRIADGPIISAINAAWTAQTGDQNKLIVAGTDDDLWQSYLSVKTQAAADFVKNYL
                     WNHSATAVLYNNDGVNRGTEQVAHSGLAQIYAGHEAADFFGVPYADPRYPDVFGRVQV
                     GTVYTTGTKIAEHGGDNPGDRDVPLLVYAPGTVSHSTVTSWVETTQVAPTILHLLGLD
                     PNALKAVQEEGTEVLPGID"
BASE COUNT          555 a         1068 c         1080 g          542 t
ORIGIN      
        1 cctgactttc gcccgcgcca cctagagcgg cgtgcgtgag cctctccatc agaccggctt
       61 gtgtgtcgcg gctgtgcgcg aatagttcga acaggatcag cccctggcgg acgagtacca
      121 gcacgaccag cccgaacgcc ataacccaag ccgcgtggtc aagttgatgg ccgagcctga
      181 tctgcacggc ggcgacggcc agcgcaagca acaccggcac tagcggggat atcagcgacg
      241 ccagcgatgg cctcgcgcgc tcggcgcgca ggaccctgcc gctgctcggc tgcgagctga
      301 acgcggcgag tgcgatcccg agatatgccg cgacccaacc ggtgtcgatc agacccggac
      361 tcgcgtaact cggtgaagcg gtgaggtagg tgaaggtgct gtccgagacc gccatcgcca
      421 gaagaccagc caacaggcac cacagcgaca gacggtcgcc gctggtcatc ccgcgcatcg
      481 caagcacgat gaaaaagacg atcaccacgt cgccaaacgg ataggccacc gccacgatgc
      541 cagaccatgt atcaagattg gtgctccgcc acagcgagcc gagcacgagc gtccagctga
      601 gaaacagcag cgcggcggcg acgaccaggc cgtcgaacag cgagcgggtc ttgcgtgtgc
      661 cgctgccgtg tgtcgccggg tgcacgacca gtgccgcgac cgcgagcggg atcgccgaga
      721 gatagccgat atccgcccac gacggcaccg gcacagccgc gtcgagggcc agcgcgtagt
      781 agccccagat gacctccgcg accgtccagc acgccatggc caagccgagc agcaaccaaa
      841 acagacgcat ccgcccgtcg tggcgagcac gagcgcgaaa acacagccca catccgatca
      901 acgccgccag tggggtcgca agatcatcga ccaagagcgt gacctggcca ccgccgacgc
      961 gcaatccgac ccaggccgcg aacacgcccg tcgcgagcac gacaacacct actgcggcgg
     1021 gaaagcgccg gctactgaga gtgtgctcca tcacgcacac gtatcggtcg ccgcagtcta
     1081 gggggcgcac tttcgtccca aaaagtggac ccctaggccc gccgctcacc cgcccctgtt
     1141 ccccacgcgt gcatcgcgag cccattgggt aacaagccga ttctgttacc cgtatcggcg
     1201 tctatcggcc gatatgcgac cctatccgcc ggacgccgga ccgacgcgaa tcccagcaaa
     1261 cacgccgttt ctccggctgt atggcgtttc ccaggtcgcc tccggagcga gaggtcgcag
     1321 gttcaaatcc tgccgggcgc ggttgcgatt gagcctgcaa tcgcgggcct tctagcggat
     1381 gcggcatgcc aggacaccgc cgcgagcatg ccagcatcgg cgttagcgcc tgctcccggc
     1441 ccggggtgct accaatgcca ggttgcaccc cgggaggagc ggaatggcta gtcgatgccg
     1501 gggaggacct ccgtgccctc ctcctggacg gccttcagcg cgttcggatc cagaccaagg
     1561 aggtggagga tcgtcggtgc cacctgggtc gtctccaccc aggaggtgac cgtcgagtgc
     1621 gaaaccgtcc ccggcgcgta gacgagcagc ggcacgtcgc ggtcacctgg gttgtcgcca
     1681 ccgtgctcgg cgatcttggt gccggtggtg tacaccgtgc cgacctgcac gcgaccgaac
     1741 acgtccgggt agcgcgggtc cgcgtacggc acgccgaaga agtcggcggc ctcatgaccg
     1801 gcgtagatct gcgccagacc ggagtgcgcg acctgctcgg tgccgcggtt cacgccgtcg
     1861 ttgttgtaga gcacggccgt ggcgctgtga ttccagaggt agttcttgac aaagtcggcc
     1921 gcggcctgag tcttgaccga caggtagctc tgccagaggt cgtcgtcggt gccggcgacg
     1981 atcagtttgt tctggtcacc ggtctgcgcg gtccaggccg cgttgatcgc gctgatgatc
     2041 ggcccgtccg cgatccgctt gagctgcagc gggtcctgcg gcgactgccc gtgcttggcg
     2101 gtgatgatga tcgccgtggt cgacgccaga ccgtcctgct ggatcgtgtc ggccatctgc
     2161 tggagctggg cgttgacgta gtcgaatgcg ctggccagca gcgggcccgg cgtcgttccg
     2221 ccggggaggt agccggcgag ctccgtcggc ccctccgtgt agctgccgtt tgcgttctcg
     2281 gtgagggtgc tgggcgagtc gaccttctca gcgaccgaca cggtctggaa gttcatccca
     2341 aggatcgccg gcacaccgac cttctgggta ccgctgtggt cgtagccgtt cagctcgttg
     2401 atgaccgcct gcaccttgta accgtcgtac tgcttggtcg cggcgtcatc gtgcgcccaa
     2461 tccgtgtcgg tcgggtacgg cacgccgtta ggctcgaccg cctgcgagtc gatctcggga
     2521 ccgaagaagt cgtcgatgct cgtgccaccg gaaccgggcc cgttgaacga cgtgtagatc
     2581 gcgtgcttgt cagaccaagc tgtgcgcagg ccggcgctgt ggatcacctg gaagatcgtg
     2641 ttgaccttga ggtagtccca cggcatgatc ggctgacagg tcgtcgggtc gaccgggaag
     2701 ctccccgtgt tcaggcccga ctgcggatgc gcggacagat tcatgatcgc tgccggtttc
     2761 gtgtccacac cgttgtggta gatcgagccg ccctcgtcga aggacgggaa cgtgttgcca
     2821 ctgccgttgt tgagcaggtc aggcacagcg ttgagcgtgt cgtccggcga gtcgtagatg
     2881 acgtccccgc cggtcgccgg ctgccccggc gtgcaggcgg cgcccgcctc gtccacggcg
     2941 tggctgtact cgacgtcgta gtacacgccg gttgctcgcg gatcgccgcc ggtcatcagc
     3001 gcggtgccgc ccggatcgga gtccgacggg tccgacgtgt ggttgttggt gaactcagcg
     3061 ccaccggagg cgagctgggc gagctccgag ctcggattgt tggcgatgta ccagtcaagg
     3121 tcggactggt gcataccgtc gaccgagatc agcaccgcat gcttgatctg gcctttgtga
     3181 tgggcggatg cgctcggcgc cgagatggcg acggtccccc cgattgcaac agcggcgatg
     3241 gcggc
//
LOCUS       DATCBJ010000058         4278 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_471516, whole genome shotgun sequence.
ACCESSION   DATCBJ010000058 DATCBJ010000000
VERSION     DATCBJ010000058.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 4278)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4278)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4278
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_471516"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..202
                     /locus_tag="VII50_01300"
     CDS             <1..202
                     /locus_tag="VII50_01300"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009072889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008725 - DNA-3-methyladenine glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HEY5049505.1"
                     /translation="APTRRRGAPKTLADVPAISPESTAMAKELKRRGFQFVGPTTAYA
                     HMQATGMVNDHLATCHARKLCP"
     gene            complement(228..1055)
                     /locus_tag="VII50_01305"
     CDS             complement(228..1055)
                     /locus_tag="VII50_01305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEY5049506.1"
                     /translation="MSEFPADAGRRHGVELHHGVELAIVDAVATVTIASDRGVLSTAT
                     KTALRDVLNRLADDPAVRAVVLTGSGATFCVGQDLKEHAAALSADATAAWDTLQDHYI
                     PIVTALATMPKPVVAGVNGTAAGAGAAFAFACDLRLVSDVAVFHLAFAGIGLSVDSGL
                     SWTLPRLVGFGRATDLLLRGRPLSASDALALGLATEVVPAGDLATRAATVAAELAAGP
                     TVAFGATKQALAYSAGHGLADALDMEAGMQTLAGTTEDHADAVRAFLDKRPPSFRGR"
     gene            1258..1425
                     /locus_tag="VII50_01310"
     CDS             1258..1425
                     /locus_tag="VII50_01310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002514062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3117 domain-containing protein"
                     /protein_id="HEY5049507.1"
                     /translation="MAAMKPRTGDGPLEVTKEGRGIVMRVPLEGGGRLVVELSADEAS
                     ALGDALKNVVG"
     gene            1553..3061
                     /locus_tag="VII50_01315"
     CDS             1553..3061
                     /locus_tag="VII50_01315"
                     /inference="COORDINATES: protein motif:HMM:NF002073.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M17 family peptidase N-terminal
                     domain-containing protein"
                     /protein_id="HEY5049508.1"
                     /translation="MPPTVKLADAVPPGAAAVVVAITPGTGDPATAKLGRGAAADIER
                     FGADPAGLLAVLAREKTKGNVGDIATLPVGARLVLAAGLGGGTPRDYRRAGAAIARRL
                     KSVESAAITVGRRSSPAQVRALAEGLLLGGYSFKVTNSPKPETLMSITLVVDQPSDAI
                     ADALAHGVVTATASALARDFANMPSSTKTPAWLAKQAAGLARTSGLEVRVRDDRELRA
                     EGFGGVVAVGMGSTSPPRLIELTYTPDPAIAVDGHVVLVGKGITFDSGGLSLKPNAGM
                     IAMKTDMAAGGAIIAAMSVMRELGVRVQVTGLVPAAENMPSGSAQRPSDVITQYGGMT
                     VEVLNTDAEGRLVLADALAYADAALDPDVIVDLATLTGAIASGLGKRHAGMFTEDDKL
                     AAVLTAAGADSGEAVWRMPFVEDYAAALESSVADMSNVAIDGGRAFSGGSIIAALFLR
                     EFVGRRRWAHLDIAGVGRAEADEHEATKGPTGFGVRLLLHWLCAPDPLRGLR"
     gene            complement(3068..3595)
                     /locus_tag="VII50_01320"
     CDS             complement(3068..3595)
                     /locus_tag="VII50_01320"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007589634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HEY5049509.1"
                     /translation="MPGGGAALRFLAALLDARAVVEIGTGCGVSGVWLLRGMNPEGVL
                     TSIDVEAEHQRLAKQAYADAGMATARTRLISGAALDVLPRLTDGAYDLVFCDGEKKEY
                     GDYLREALRLLRVGGVVAFDNATWHHRVADPAQRDPATITIRDLGKQLREEPGLVPTL
                     LPVGDGLLVAVKREA"
     gene            3932..>4278
                     /locus_tag="VII50_01325"
     CDS             3932..>4278
                     /locus_tag="VII50_01325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEY5049510.1"
                     /translation="MTDSALLPQTGPLPDSLPEPSAHDPDWAAPSWDDVVRTHGARVY
                     RLAYRLTGNAHDAEDLTQDVFVRVFRSLGSYTPGTFEGWLHRITTNLFLDMVRRRARI
                     RFESLADDASDRLP"
BASE COUNT          672 a         1445 c         1483 g          678 t
ORIGIN      
        1 cgcgccgacc cggcgccgcg gagctccgaa gacgctcgcc gacgtgccgg ccatctcacc
       61 ggagtcgacc gcgatggcca aggagctgaa gcggcgcggt tttcaattcg tcgggccgac
      121 gaccgcgtat gcgcacatgc aggcgaccgg catggtcaac gaccacctgg cgacctgcca
      181 cgcgcgcaag ttgtgcccct aggggtgcgc tgacgcaaca aacgcgatta ccggccgcgg
      241 aaggacggcg gccgcttgtc gaggaatgcg cgcactgcgt cggcgtgatc ctcggtcgtg
      301 ccggccagcg tctgcatacc ggcctccatg tcgagggcgt ctgcgaggcc gtgccccgcg
      361 gagtaggcaa gagcctgctt ggttgccccg aacgccaccg tcgggccggc cgcgagttcg
      421 gcggcgacgg tcgccgcgcg ggttgcgaga tcgccggcgg gcaccacctc ggttgccaga
      481 ccgagagcga gcgcgtcgga cgccgacagc gggcgcccgc gcaacagcaa gtccgttgcc
      541 cggccgaagc cgaccagccg tggcagggtc cacgacagcc cggagtcgac agacaacccg
      601 atgcccgcaa acgccagatg gaacaccgcg acgtccgaca ccagccgcag atcgcaggcg
      661 aacgcgaagg cagcgccggc acccgccgcc gtcccgttca cccccgcgac gaccggcttc
      721 ggcatcgtcg ccagtgctgt gacgatcggg atgtagtggt cttgcagggt gtcccaggcg
      781 gccgtcgcgt ccgccgacag cgcggcagcg tgctctttga ggtcctggcc gacacagaag
      841 gtggcgcccg aaccggtgag cacaacggcc cgcaccgccg gatcgtcggc gagtcgattc
      901 aggacgtcgc gcagtgcggt cttcgttgcc gtcgaaagga ctccgcggtc actggcgatg
      961 gtgaccgtag ccacggcatc cacgatcgcc agttccacac cgtgatgcag ttctaccccg
     1021 tggcgacggc ccgcatcggc cgggaattcg gacacagaac ctccctggaa ctgccctggc
     1081 cgggacgggg acgccgtcgc ggtttccttc tggcccctcc ttgccacgat aatcttggtc
     1141 aagacagtgc gatgggttcg gcgctcgacg tgccgacggt gcaatctggt ccggcactgg
     1201 cgtgccgcgg tgcaatctgc tgcggcacga agtgccgcac gaggaaaggg agcgcgaatg
     1261 gcggccatga agccgcggac gggcgacggt ccgctcgagg tcaccaagga gggccgcggc
     1321 atcgtgatgc gcgttcctct cgaaggcggt ggccggctcg tcgtcgagtt gtctgcagac
     1381 gaggccagcg cccttggcga cgccctcaag aatgtggtcg gctgaccaac ccacagtgga
     1441 gcgggccccg gcgaagtcca cagggacttc gttcggggcc tcaaacgtgt cgggggtagc
     1501 gtcgacctgg atctgttcac cccggcctgc ctcggctcag aaaggttcgc gcgtgccgcc
     1561 cacggtaaaa ctcgcagatg cggtgccacc tggggccgcc gcagtcgtgg tggcgatcac
     1621 gccgggtacc ggtgacccgg cgacagcgaa gcttgggcgc ggggccgcgg cagacatcga
     1681 gcgtttcggt gcggatcctg cggggctgct ggccgtcctg gcgagagaga agacgaaagg
     1741 caacgtcgga gatatcgcga cattgccggt cggtgcgcgg ctggtcctcg cagcgggcct
     1801 cggtggaggg acgccgcgtg actatcgtcg tgcgggcgcc gcgatcgccc gacggttgaa
     1861 gagcgtcgag tccgcggcaa tcaccgttgg gcgccggtcg agccctgcgc aggtccgggc
     1921 tttggccgag ggcttgctcc tcggcggcta ctcgttcaag gtgaccaact cgccgaagcc
     1981 cgagacgctc atgtcgatca cgctcgtcgt tgaccaacct tccgacgcga tcgccgatgc
     2041 gctcgcgcac ggtgtcgtga cggcaaccgc tagcgcgctc gcccgcgatt tcgcgaacat
     2101 gccctcgtcg acgaagactc ctgcgtggct ggccaagcag gccgcggggt tggcacgcac
     2161 gagcggtctc gaggtccggg ttcgtgacga ccgcgagttg cgcgcggaag ggttcggtgg
     2221 cgtggtcgcg gtcggcatgg ggtcgacgag ccctccgcgc ctcatcgagt tgacctacac
     2281 ccccgacccc gccatcgcgg tcgacggaca tgttgtgctt gtcggcaagg gaatcacgtt
     2341 cgactccggc ggactctccc tcaagcccaa tgcgggcatg atcgcgatga agaccgacat
     2401 ggcggcgggt ggggcgatca tcgcagcgat gtcggtgatg cgtgagttgg gtgtgcgggt
     2461 ccaggtcacc ggactcgttc ctgctgcgga gaacatgccg tccggctcgg cgcagcgacc
     2521 cagtgatgtg atcacccagt acggcggcat gacagtcgag gtgctcaaca ccgacgccga
     2581 gggccgactg gtactcgccg acgcgctcgc ctatgccgac gccgcgctcg atcccgacgt
     2641 catcgtcgac cttgcaacat tgacgggcgc tatcgcgagc ggccttggca agcggcacgc
     2701 ggggatgttc accgaagacg acaagcttgc cgcggtgctc actgctgccg gtgccgatag
     2761 cggcgaagct gtctggcgga tgccgttcgt cgaggactac gccgcagctc tcgagtcgtc
     2821 ggtcgccgac atgtcgaacg tggctatcga cggtggccgg gccttcagcg gcgggtcgat
     2881 cattgcggca ctgttcctac gagagttcgt cggacgccga cggtgggcgc acctcgatat
     2941 cgccggcgtc ggtcgggccg aggcggacga acacgaagcc accaaggggc cgacgggttt
     3001 cggcgtccgg ctgctgttgc attggttgtg cgcacctgat ccgctgcgtg gcctgcggta
     3061 gcccttctta cgcctcgcgc ttcacggcga ctaacagacc gtcccctacg ggtagcaggg
     3121 tcggcaccag gccgggctct tcgcgtagct gcttgcccag atcgcggatc gtgatcgtgg
     3181 ccgggtcgcg ctgcgcggga tcggcaaccc ggtggtgcca ggtcgcgttg tcgaaggcaa
     3241 ccactccgcc gacccgcagc agccgcagtg cctcgcgcag gtagtcgccg tactccttct
     3301 tctcgccgtc gcagaagacg aggtcgtaag caccgtcggt gagtcgcggc aggacgtcga
     3361 gtgcggctcc cgaaatcagc cgcgtacgtg ccgtcgccat cccggcgtcc gcgtaagcct
     3421 gcttggctag ccgttggtgc tcggcctcca cgtcgatcga ggtgagcacg ccttcggggt
     3481 tcatgccgcg cagcagccag actcccgaca caccgcaacc ggtcccgatt tcgacgacgg
     3541 cgcgggcgtc aagcaacgcc gcgaggaatc gaagcgcggc cccgccgccc ggcatgatcg
     3601 gcgtggcgcc gagttcggcg ccacgcgcgg acgccgccgt cagaacgtcg tcctggccga
     3661 tgtaggcctc ggtgtagtcc cacgtcgatt gcagaccgga gatggcggcc tcccgagaga
     3721 gtcgtggtca tgtgatggca ccgtgcgggg cgccgttgac cttcagcgca gcgtagcccg
     3781 cccgaggtcg tcgaactgcg ggaacccggc ggggcgactt tcggggaacc cgaccggagg
     3841 cacagtcgtt gcgcttctca ggaggacatg tgacggccat caggattccc tcaggatcgg
     3901 ggcgcaggat gccagcggag gtgtccccct gatgacagat tccgccctgc ttccgcagac
     3961 tggcccactg cccgattcgc tgcccgagcc atcggcccac gacccggatt gggccgcgcc
     4021 cagctgggac gacgtggtgc gcacgcacgg tgcccgggtg taccggcttg cctaccggtt
     4081 gacaggcaac gcccacgatg ccgaagacct cacccaggac gtgttcgtcc gggtgtttcg
     4141 atcgctcggg tcgtacaccc ccggcacctt cgaggggtgg ctgcaccgca tcaccacgaa
     4201 cctcttcctc gacatggttc gccggcgcgc gcggatccgc ttcgagtccc tcgccgacga
     4261 cgcctccgac cggctccc
//
LOCUS       DATCBJ010000059         3254 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_220529, whole genome shotgun sequence.
ACCESSION   DATCBJ010000059 DATCBJ010000000
VERSION     DATCBJ010000059.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 3254)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3254)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3254
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_220529"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..272
                     /locus_tag="VII50_01330"
     CDS             <1..272
                     /locus_tag="VII50_01330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5049511.1"
                     /translation="SEGIQRTARYAGAPQKYHATVSRAWVELVGHHVDAHPGTDFDAF
                     VERNVALLDKRLLTRFYRPATLAAAPARDGWVEPDLAPFPWQQQS"
     gene            305..736
                     /locus_tag="VII50_01335"
     CDS             305..736
                     /locus_tag="VII50_01335"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEY5049512.1"
                     /translation="MIAIASAHVVATASPSAIFERWADVATWPQWNADTEWVRLDGAF
                     TQGGTGVLKPKGGPKVKFVIDKLTGTEFIDVSRLAGARLRFSHVVTPASDGCVVDVAI
                     TMSGPLAWLWNRLLGKGLAASVQPDLDALARAAESGHTLAQ"
     gene            733..1170
                     /locus_tag="VII50_01340"
     CDS             733..1170
                     /locus_tag="VII50_01340"
                     /inference="COORDINATES: protein motif:HMM:NF013231.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEY5049513.1"
                     /translation="MTRLASEHESADDSTGLLLWQVTNRWQAAQRAALKPFDLTHVQF
                     VLLASLTWLDADGPVTQRQLADHAVTDPMMTSQVLRVLQGRGLVERVVHPADKRAWSL
                     TATTAGVKLANRANAAVEACDRQFFFALEGDRAEFTAALRRLR"
     gene            complement(1183..2835)
                     /locus_tag="VII50_01345"
     CDS             complement(1183..2835)
                     /locus_tag="VII50_01345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009083984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair helicase XPB"
                     /protein_id="HEY5049514.1"
                     /translation="MTEGPLIVQSDKTLLLEVEHELAAECRRAIAPFAELERSPEHVH
                     TYRLTPLGLWNARAAGHDAEEVVDALLKYSRYAVPHALLIDVAETMARYGRLQLTNDP
                     AHGLVLRSVDRAVLEEVLRSPKVAPMIGVRLDDDAVVIHPSERGHLKQVLLKLGWPAE
                     DLAGYVDGEAHAISLHEDGWTIRPYQLEAVEGFWHGGSGVVVLPCGAGKTIVGAAAMA
                     KAQATTLILVTNTVAARQWRHELLKRTSLTDDEIGEYSGAKKEIRPVTIATYQVMTTR
                     RKGVYAHLELFDERDWGLIIYDEVHLLPAPIFRFTADIQSRRRLGLTATLVREDGREG
                     DVFSLIGPKRYDAPWKDIEAQGYIAPADCVEVRVTLTEHERLLYATAEPEERYRLAST
                     TETKMRVIEALAARHVGDQLLVIGQYLDQLDELGERLGVPVIKGDTTVKERERLYDLF
                     RTGEITTLVVSKVANFSIDLPEASVAIQVSGSFGSRQEEAQRLGRILRPKHDGRTARF
                     YAVVARDTIDQDYAAHRQRFLAEQGYAYRIIDADDVLAGDEI"
     gene            complement(2832..>3254)
                     /locus_tag="VII50_01350"
     CDS             complement(2832..>3254)
                     /locus_tag="VII50_01350"
                     /inference="COORDINATES: protein motif:HMM:NF024676.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HEY5049515.1"
                     /translation="RPRPLRRRPELAAPAASVVTVAVKAIRGGDRAVTSTRGTVVGSA
                     ISDVVPHSASSETLAILQDAVAAGRAVWIGYLNAQGQASNRIIEPQRLAGGYLTAFDY
                     RRDEARTFAVHRITGVAELTGDEIAENADEPTPGTMVQ"
BASE COUNT          533 a         1072 c         1073 g          576 t
ORIGIN      
        1 tgagcgaagg aattcagcgc actgcacgat atgccggagc gccgcagaag tatcacgcga
       61 ccgtcagccg cgcctgggtc gaactcgtcg gccatcatgt cgacgcccac cccggcaccg
      121 acttcgacgc gttcgtcgaa cggaacgtcg cattgctcga caagcgactg ctgacccgtt
      181 tctaccgccc ggcgacgttg gcggccgcgc ccgcgcggga cggctgggtc gagcccgacc
      241 tcgcgccctt tccgtggcag cagcagtctt gacatcacag aagctaagcg cgcatactaa
      301 acccgtgatt gctatcgcct ctgcgcacgt tgtcgccact gcatcaccgt ccgccatctt
      361 cgaaagatgg gccgacgtcg ccacctggcc gcagtggaat gccgacaccg aatgggtccg
      421 actcgacgga gcattcacgc aaggcggcac cggggtgctg aaacccaagg gtggcccgaa
      481 ggtcaagttc gtcatcgaca agctgaccgg caccgagttc attgacgtct cacgccttgc
      541 cggcgcgcgg ttgaggttca gccatgtcgt cacaccagcc tccgacggct gcgtcgtcga
      601 cgtcgccatc accatgagcg ggccgctcgc gtggctgtgg aatcgcctcc tcggcaaggg
      661 ccttgccgcg tccgttcaac ccgacctcga cgcgttggcg agggcagcgg agagcggtca
      721 cacgctggcg caatgacgcg acttgccagt gagcatgaaa gcgccgacga cagcaccggc
      781 ctgctgctct ggcaggtcac gaaccgctgg caagcagcac aacgcgccgc tctcaagccg
      841 ttcgacctca cgcacgtgca gttcgtcctt cttgcgtcac tgacatggct ggacgccgac
      901 gggccggtga ctcaacgaca gctggccgac catgcggtga ccgatcccat gatgacctcg
      961 caggtgttgc gggtacttca agggcgcgga ctcgtggagc gcgtcgtgca ccccgccgac
     1021 aagcgtgcgt ggtcgctcac cgcgacgacc gcgggcgtga agctggccaa tcgcgcgaac
     1081 gcggcagtcg aggcatgcga ccgccagttc ttcttcgctt tagaaggaga tcgagccgag
     1141 ttcacggcag cgctacggcg gctgcgctga ccggcgtccg gcctaaatct cgtcgccggc
     1201 cagcacgtcg tcggcgtcga tgatgcggta tgcgtagccc tgttcggcaa ggaatcgctg
     1261 gcgatgcgcg gcgtagtcct ggtcgatggt gtcgcgcgcg acgaccgcgt agaaccgtgc
     1321 ggtgcgaccg tcgtgcttgg gccgcaatat ccggccgagg cgctgcgctt cctcctgccg
     1381 tgatccgaac gatcccgaga cctgaatcgc caccgatgcc tcgggcaggt cgatcgagaa
     1441 gttcgcgact ttcgacacga cgagcgtggt gatctcaccg gttcgaaaca ggtcgtacag
     1501 tcgctcgcgt tccttcaccg tcgtgtcgcc cttgatgacc gggacgccga gacgttcgcc
     1561 taattcgtcg agctggtcga ggtattggcc gatgacaagc aactggtcgc caacgtggcg
     1621 agcagcgagc gcctcgatca cccgcatctt cgtctcggtc gtcgacgcca gccggtagcg
     1681 ctcctcaggt tcggcggtcg cgtagaggag ccgctcgtgc tcggtgaggg tgacgcgcac
     1741 ctcgacgcaa tcggccggcg cgatgtagcc ctgagcctcg atgtccttcc agggtgcgtc
     1801 gtagcgcttc ggaccgatca ggctgaacac atcgccctcg cggccgtcct cgcgcacgag
     1861 cgtcgcggtg agcccgagcc gccgccgcga ttggatgtcg gccgtgaacc gaaagatcgg
     1921 cgcgggcagc aggtgcacct cgtcgtagat gatcaggccc cagtcgcgtt cgtcgaagag
     1981 ctcgagatgc gcgtagacgc ctttgcgacg ggtcgtcatg acctggtacg tcgcgatggt
     2041 gaccggccgg atttcctttt tggcgccgga atactcgccg atctcgtcgt cggtcagtga
     2101 cgttcgtttg agcagttcgt gtcgccactg acgagctgcg acggtgttcg tcacgaggat
     2161 caaggtggtc gcctgcgcct tggccatggc agccgcgccg acgatggtct tgccggcgcc
     2221 gcacggcagc acgacgacgc ccgacccgcc gtgccagaaa ccctcgaccg cttcgagttg
     2281 gtagggccgg atcgtccatc cgtcctcgtg cagggagatc gcgtgcgcct cgccgtcgac
     2341 ataaccggcg aggtcttcgg ccggccagcc gagcttgagc agcacctgct tgaggtgacc
     2401 tcgctcgctc ggatgaatga cgacggcatc gtcatcaagc cggacgccga tcatcggcgc
     2461 aaccttcggg ctgcgcagca cttcttcgag caccgcgcgg tcgacggagc gcagcacgag
     2521 gccgtgcgcg ggatcgttcg tgagctggag tcggccgtag cgggccatcg tctcggcgac
     2581 gtcgatcagt agcgcatggg gcaccgcgta gcggctgtat ttcagcagtg cgtcgacaac
     2641 ttcttcggcg tcgtgtccgg cagctcgcgc attccatagg ccgagcgggg tgaggcgata
     2701 ggtgtggacg tgctcgggag aacgctcgag ttctgcgaac ggcgcgatcg cccgccggca
     2761 ctccgctgca agttcgtgct cgacctcgag cagcagcgtc ttgtcggatt gcacgatcag
     2821 cggtccctcg gtcactggac cattgtccca ggtgtcggct cgtccgcgtt ttcggcgatc
     2881 tcgtcaccgg tgagttcggc gactccagtg atccggtgta ccgcgaacgt ccgcgcctcg
     2941 tcgcgtcgat agtcgaatgc cgtcaggtat ccgccggcca ggcgttgcgg ttcgatgatg
     3001 cgattgctgg cttgaccttg ggcgttgaga tagccgatcc agacggcacg gccggccgcg
     3061 acggcgtcct gcaggattgc caaggtctcg ctggacgccg aatggggcac gacgtccgaa
     3121 atcgcactgc ccacgacggt gccgcgagtc gacgtcacgg cgcggtcgcc gccgcggatc
     3181 gccttgaccg cgacggtgac gaccgaagcc gccggcgccg caagctcggg acgccgccga
     3241 agcggtcgcg gtcg
//
LOCUS       DATCBJ010000060         2463 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_544380, whole genome shotgun sequence.
ACCESSION   DATCBJ010000060 DATCBJ010000000
VERSION     DATCBJ010000060.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2463)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2463)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2463
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_544380"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..548
                     /locus_tag="VII50_01355"
     CDS             <1..548
                     /locus_tag="VII50_01355"
                     /inference="COORDINATES: protein motif:HMM:NF013989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HEY5049516.1"
                     /translation="LADVVIAGAGTVAAEGYGPARAREQYQELRAAAGQQPAPTMVVV
                     SQRLQLDFESKYFAQASQPPIVVTSAAAPADRLLAARKVAEIVIAGDSAVSPALMVDA
                     LVARGHRRLLCEGGPTLLGTVAADGVLDELCLTIAPMLVGGPSRRVLEGPVLDPAPKL
                     ALTQLLQDDDDLLYARYEVQR"
     gene            560..1621
                     /locus_tag="VII50_01360"
     CDS             560..1621
                     /locus_tag="VII50_01360"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003910 - DNA ligase (ATP) activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA ligase"
                     /protein_id="HEY5049517.1"
                     /translation="MPPVKPMLAKSQPAIPAGMVYEPKWDGFRCIVFRDGDEIELGSR
                     NEKPLTRYFPEVVEAVRASVPQRCVLDGEIVIVSGNSLDFEALLQRIHPAASRVKKLA
                     VETPATFVAFDLLALGDESFVDEPFARRREALEEVLGSTASSVLLTPATDDLELANRW
                     FEVFEGAGLDGVVAKSRDLPYRQDQRIMVKVKHERNADCVVAGFRWHRASPEGTAVGS
                     LMLGLYGDNGVLQHVGVCGAFSAVRRRELIAELEPYRTDDGSHPWGAADADAAETGEP
                     DRRPGGVTRWNAGKDLTWVPLRPELVCEVAYDHMEGGRFRHTAQFRRWRPDRDPASCS
                     YDQLDRPVNFDLASVLTGG"
     gene            complement(1618..>2463)
                     /locus_tag="VII50_01365"
     CDS             complement(1618..>2463)
                     /locus_tag="VII50_01365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5049518.1"
                     /translation="SARAGSSSAASTSGFTSPTPLVSIGSPAPTDAAESSRVQAVTEL
                     AQRLLAEMVLAVPGGSKELGGQPVGVLTAPMTLYDDDVIDVADWWTVPETADAVLTYV
                     QHHLPAGLTSEGTGGGPVGAEVVFDRTPAGPADEGATVYVAVAPDPAGGTDLRVDVQD
                     RWQPVRPALEQVPSTVTGATLVRRAGLRETPLPPVTIHVGADIARHIAALLNALPSEA
                     GGAPDAGGGADTTTTVSFDGDPGNVQYVVAGSFYNAVTVDAPSQAMPTLSGAGDLDSY
                     LEGLF"
BASE COUNT          392 a          835 c          813 g          423 t
ORIGIN      
        1 cgctcgccga tgtcgtgatc gccggtgccg gcacggtggc tgccgaaggg tacggtcccg
       61 cccgcgcccg cgagcagtat caggagcttc gcgcggcagc cggtcagcag cccgcgccca
      121 ccatggtggt cgtgtcgcag cggctacagc tcgactttga atcgaagtac tttgcgcagg
      181 catcgcagcc gccgatcgtc gtcacatccg cagctgcgcc cgcggaccgc ctcctcgccg
      241 cacgcaaggt ggccgagatc gtcatcgccg gcgacagtgc tgtctccccc gcccttatgg
      301 tcgacgcgct cgttgcgcgc ggtcaccgac ggctcctatg tgagggcggg ccgacactgc
      361 ttggaacagt ggcagctgac ggcgtcctcg atgaattgtg tctgacgatc gcgccgatgc
      421 tggtgggcgg gccatcgcga cgcgtcctcg agggaccggt tctcgatccc gctccgaagt
      481 tggcgctgac ccagttgttg caggacgacg acgacctgct ctacgcgcgg tatgaggtgc
      541 agcgatgaag cttccagtga tgcctcctgt caaaccgatg ctcgccaaat cccagcccgc
      601 catcccggcc ggcatggtgt acgagccgaa gtgggacggg tttcgctgca tcgtctttcg
      661 cgacggtgac gagatcgaac tgggcagccg caacgaaaag ccgttgaccc ggtactttcc
      721 cgaggtcgtc gaagcggtgc gcgcgagcgt gccgcagcgc tgcgtgctcg acggcgagat
      781 cgtgatcgtc tcgggcaact ccctcgactt cgaggccctg cttcaacgca tccaccccgc
      841 tgcgtcacgc gtgaagaagc tggcggtaga aactccggcg acctttgtcg cattcgatct
      901 gcttgcgctg ggcgacgaat cctttgtgga cgagccgttt gcgaggcgcc gggaagcact
      961 tgaagaagtg ttgggctcga cggcgtcctc ggtgttgctg acaccggcga ccgatgatct
     1021 cgagctcgcg aatcgatggt tcgaggtctt cgaaggtgcc gggcttgacg gcgtggtcgc
     1081 aaagtcgcgg gacctcccct accgccagga ccagcgcatc atggtgaagg tcaagcacga
     1141 gcgcaatgcc gactgcgtcg ttgccggttt tcgctggcat cgggcatcac cggaaggcac
     1201 cgcggtcggc tcgctgatgc tcggtcttta tggcgacaac ggggtgctcc agcatgtcgg
     1261 cgtgtgcggc gcattctccg ccgtccgccg ccgcgaactt atcgcggagc tcgagccata
     1321 tcgcaccgac gatgggtcac acccctgggg tgcggccgat gccgacgcag ccgaaaccgg
     1381 cgagcccgat cggcgtcccg gcggtgtgac tcgctggaac gcgggcaaag acctcacctg
     1441 ggtgccgcta cgccccgaac tcgtgtgcga ggtcgcctac gaccacatgg aaggcggccg
     1501 gttccggcac acggcccagt ttcggcgctg gcggcctgat cgcgaccctg cctcgtgcag
     1561 ctacgaccaa ctcgacaggc cggtcaactt cgacctcgcc agcgtcttga ccgggggcta
     1621 gaacaagcct tcgaggtagc tgtccaggtc accagcgccg gacagcgtgg gcatcgcttg
     1681 gctgggagcg tcgaccgtaa cggcgttgta gaagctaccg gcgacgacgt actgcacgtt
     1741 gcctgggtcc ccgtcgaagc tgacggtggt cgtcgtgtct gctccgccgc cggcatccgg
     1801 tgctccgcct gcttcggaag ggagcgcgtt gagcagcgcg gcgatgtgac gtgcgatgtc
     1861 ggcaccgacg tgaatagtca ccggcggcag cggagtttcc ctgaggccgg cgcgccgcac
     1921 cagtgtggca cccgtcacgg tagaaggcac ttgctcaagc gccggacgca ctggctgcca
     1981 gcggtcctgc acgtccaccc gcagatcggt gccacccgca ggatcgggag ccaccgcgac
     2041 gtacaccgtc gcaccttcgt cggcaggccc ggccggcgtc cgatcgaaga cgacctcggc
     2101 gcccaccggg ccgccaccgg tcccctccga cgtcagccca gcaggcaaat ggtgttgaac
     2161 gtacgtcagc acggcgtcgg cagtttccgg caccgtccac cagtcggcaa catcgatcac
     2221 gtcgtcgtca tacaacgtca tcggcgcggt cagaactccg accggctgcc cacccaattc
     2281 cttcgaaccg cccggcaccg ccagcaccat ctcggcgagg aggcgctggg ccagttccgt
     2341 gactgcctgt acccgtgaac tctcggccgc gtccgttggc gcgggacttc cgatgctgac
     2401 cagcggggtc gggctcgtga agccgctggt gctggcggcc gagctgctcc cggctcgcgc
     2461 cga
//
LOCUS       DATCBJ010000061         5421 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_544379, whole genome shotgun sequence.
ACCESSION   DATCBJ010000061 DATCBJ010000000
VERSION     DATCBJ010000061.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 5421)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5421)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5421
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_544379"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1460
                     /locus_tag="VII50_01370"
     CDS             <1..1460
                     /locus_tag="VII50_01370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-1,4-glucan--maltose-1-phosphate
                     maltosyltransferase"
                     /protein_id="HEY5049519.1"
                     /translation="LLARHPVRTLLTVSPSYPLWVDRERALFGSWYEFFPRSEGATLN
                     PPRSGTFATAAKRLPAIAAMGFDVVYIPPVHPIGTTFRKGPNNTLTPGPGDPGVPWAI
                     GSSEGGHDTIHPDLGTMEDFDAFVAAAANSGLEVALDLALQCSPDHPWVTQHPEWFTT
                     RADGSIAYAENPPKKYQDIYPLNFDNDYEGLYAEIVRVVRLWMSHGIRIFRVDNPHTK
                     PVRFWEELLADIRSTDPDVLFLAEAFTRPAMMRGLAKVGFHQSYTYFTWRNTRPELEL
                     YLDELSHETSDVLRPNFFVNTQDILHAFLQQGGPPAFKIRAVLAATGSPTWGVYSGYE
                     LFENTPLRPGSEEYLDSEKYQYRPRDYAAAEAAGRSLAGYITTLNRIRREHVALQRLR
                     NLHLHGADNAQIITYSRQVVTDDATDTILVVVNLNPFNVSEATLALDMPALGVDWGAQ
                     LEVIDQLSGARYTFGQYNYVRLDPHVEPAHIFVVS"
     gene            1548..3227
                     /gene="treS"
                     /locus_tag="VII50_01375"
     CDS             1548..3227
                     /gene="treS"
                     /locus_tag="VII50_01375"
                     /EC_number="5.4.99.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047471 - maltose
                     alpha-D-glucosyltransferase activity [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose alpha-D-glucosyltransferase"
                     /protein_id="HEY5049520.1"
                     /translation="MTDPVADSAEHSVDPHWYKRAVFYEVLVRGFADSNGDGTGDLKG
                     LVSKLDYLSWLGIDCIWLLPIFQSPLRDGGYDISDYFTVLPEFGDLGDFVELVEEAHK
                     RGMRVIADLVMNHTSDQHPWFQASRSDPTGPYGDYYVWSKTDTSYRDARIIFVDTEKS
                     NWTFDPVRGEYYWHRFFGHQPDLNFENPAVQDAMLGTLRFWLELGIDGFRLDAVPYLF
                     EEEGTNCENLPRTHEYLKRVRKEIDRDFPDRVLLAEANQWPADVVQYFGDSDECHMAF
                     HFPLMPRIFMSVRREQRYPISEILSQTPPIPDGCQWGIFLRNHDELTLEMVTDEERDY
                     MYTEYAQDPRMKANIGIRRRLAPLLENSRDQMELFTALLLSLPGSPVLYYGDEIGMGD
                     NIWLGDRDGVRTPMQWTPDRNAGFSNCDPGRLYLPVIMDPIYGYQGLNVEAQMRTSTS
                     LLHWTRRMIDTRKRHPTFGAGNFAELGASNPSILAFVREFGDDRVLCVNNLSRFPQPV
                     ELDLRRYEGVVPVEMLGGMHFPKVGELPYLLTLPGHGFYWFTLPAKRAEDV"
     gene            3228..4640
                     /locus_tag="VII50_01380"
     CDS             3228..4640
                     /locus_tag="VII50_01380"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HEY5049521.1"
                     /translation="MSTKKVDRTQLEDFLRDWLPKQRWFAGKDRGAKAVHIATERVLL
                     AGDPSLLHLFVRVEQNPAVNPLPDLEPAGDSVTTTYQLLLGVRSTLPPELEHASIGQV
                     VDLGWAYEASQDHDLMHTMLGWVAGEETRGDIVFALEPGAKIDPDAVSLVLGGEQSNT
                     SVVFGEQTIMKLFRIVVPGLNPDLEVTRALTEMGNLHVPALQGWIESDVEGTASTLGL
                     LQEFLRTGSDGWALALTSVRDLFAEGDLHAGEVGGDFAAESERLGMATADVHSAMAQA
                     LPTGIADQHALETMAQSMRKRLDAARIAVPELADHVVAISAVFDALANSREPVTLQRV
                     HGDFHLGQVMRTDNGWVLLDFEGEPGAPIAARTALSSPLRDVAGMLRSFDYASRYLLA
                     DQPAAAQLEYRAAEWAERNRDAFCTGYAKSAGRDPREDDVLLRAFELDKAVYEVAYEA
                     RNRPSWVRIPLGSIERLVAA"
     gene            4637..>5421
                     /locus_tag="VII50_01385"
     CDS             4637..>5421
                     /locus_tag="VII50_01385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5049522.1"
                     /translation="MTSGPMPPKKDEPEAPVTAQPAATVVPEKKVATVTKPADTKPAD
                     TRLASVPRAPVTAPPEEIDRLVSGAHHNPHGILGAHLAGGVVTFRSFRPNAREVTVLV
                     DLLRIPLAHERGGVWSATWTSSAVPDYRVEVVYDDGIPHIVDEPYRFLPTLGEIDLHL
                     IGEGRHEQLWEVLGAHEHHYDTPSGVVNGTSFAVWAPNARAMRVAGDFNFWDSQANPM
                     RSLGSSGVWELFIPGVASGDRYKLNVLGSDGQWREKADPMAFAT"
BASE COUNT          979 a         1826 c         1687 g          929 t
ORIGIN      
        1 cgcttcttgc acgccaccca gtgcgcaccc tgctgaccgt gtcgccgtcc tacccgctat
       61 gggtcgatcg cgagcgcgcg ctcttcggct cctggtacga gttcttcccg cgctcggaag
      121 gcgcgacgct caacccgccg cggtcgggca cgttcgcgac tgcagcgaaa cggctgccgg
      181 cgatcgcagc gatgggattc gacgtcgtct acatcccgcc ggtgcatccg atcggaacga
      241 cgtttcgcaa gggaccgaac aacaccctca cgccaggccc aggcgatccc ggagtcccgt
      301 gggccatcgg gtcgagtgag ggcgggcacg acacgatcca tcccgacctc ggcacgatgg
      361 aggacttcga cgcgttcgtc gcggcggcag ccaacagcgg cctcgaggtt gcgctcgacc
      421 tggcgctgca gtgctcaccc gaccatccgt gggtaaccca gcatccggag tggttcacca
      481 cccgcgccga cggaagcatc gcctatgcgg agaacccacc gaagaaatac caggacatct
      541 acccgctgaa cttcgacaac gactacgaag gcctctacgc cgagatcgtg cgggtcgtcc
      601 gcttatggat gagtcacggg atccggattt tccgggtcga caacccgcac accaagccag
      661 ttcgcttctg ggaggaactg cttgcggata ttcgctcaac cgaccccgac gtcttgttcc
      721 tggcggaggc gttcacccgg ccggccatga tgcgcggtct ggcgaaggtc ggattccacc
      781 agtcgtacac ctacttcacc tggcgcaaca ccaggccgga gctcgagctt tacctcgacg
      841 agttgtcgca tgagacctca gatgtcctgc gccccaactt cttcgtcaac acccaagaca
      901 tcctgcatgc gttcctgcaa cagggcggcc cgcccgcgtt caagattcgc gcggtgctcg
      961 ccgcgacggg ctcgccaacc tggggcgtgt actcgggcta cgagctcttc gagaacaccc
     1021 cgctccgccc aggcagcgag gagtatctcg actcggagaa gtaccagtac cgtccccgcg
     1081 actacgcggc cgccgaggca gccggccgga gtcttgccgg ttacatcacg acgctcaacc
     1141 ggatccggcg cgaacatgtc gccctgcaac ggctgcgcaa cctgcacctg cacggcgccg
     1201 acaacgcgca gatcatcacc tattccaggc aggtcgtgac cgatgatgcg accgacacga
     1261 tcctggtcgt cgtcaacctc aacccgttca acgtcagtga ggcaactctc gcgctggata
     1321 tgccggcact gggcgtcgac tggggagccc agcttgaggt catcgaccag ctcagcgggg
     1381 cgcgctatac cttcgggcag tacaactatg tacggctgga tccgcacgtc gagccggcgc
     1441 acatcttcgt ggtttcgtga ccatctgcag tgacgatcat cgttgacatc acagttcaca
     1501 tccagtgaca tccccacagt gacatcccca tagggcggcg ttgacagatg acagatcccg
     1561 tggccgattc ggcggagcac tccgtggatc cgcactggta caaacgcgcg gttttctacg
     1621 aagtgctggt ccgcggcttc gccgacagca acggtgacgg caccggcgac ttgaagggct
     1681 tggtgtcgaa gctcgactat ctgagctggc tcggcatcga ctgcatctgg ctgctgccga
     1741 tcttccagtc gccgctgcgc gacggcggct acgacatctc cgactacttc acggtcctgc
     1801 ccgagttcgg tgacctcggc gacttcgtgg agctggtcga ggaggctcac aagcgcggga
     1861 tgcgcgtcat cgccgacctg gtcatgaacc acaccagcga ccagcatccg tggttccagg
     1921 cctcgcgctc agatccgacc ggtccgtatg gcgactacta cgtgtggtcc aagaccgaca
     1981 ccagctatcg cgatgcacgc atcatcttcg tcgatacaga gaagtcgaac tggaccttcg
     2041 acccggtgcg cggcgagtac tactggcatc gattcttcgg ccaccagccc gacctgaact
     2101 tcgagaaccc ggcggtccag gacgccatgc tcggcaccct gcggttctgg ctcgaactcg
     2161 gcatcgatgg cttccggctg gacgccgtcc cctatctgtt cgaagaagag gggaccaact
     2221 gcgagaacct gccgcgcacg catgaatacc tgaagcgggt gcgcaaggag atcgacagag
     2281 acttccccga ccgcgtcctg ctggcagagg ccaatcagtg gcccgccgac gtcgtccaat
     2341 atttcggcga cagcgacgaa tgccacatgg cgttccactt cccgctgatg ccacgcatct
     2401 tcatgtccgt gcgccgcgaa cagcgctacc cgatctcgga gatcttgtcc caaacaccgc
     2461 cgatccccga cggctgccaa tggggcatct tcttgcgcaa ccacgacgag ttgacccttg
     2521 agatggtgac cgacgaagag cgcgactaca tgtacaccga gtacgcgcag gatccacgca
     2581 tgaaggcgaa catcggcatc cggaggcgat tagccccatt actggagaac tctcgggacc
     2641 agatggagct gttcaccgcc ctgctgctct cgctgccggg ctccccggtg ctctattacg
     2701 gcgacgagat cggcatgggc gacaacatct ggcttggtga ccgcgacggg gtccggacgc
     2761 cgatgcagtg gacgccagac cgcaacgccg gcttctccaa ctgcgatccc ggccggctgt
     2821 acctgccggt gatcatggat ccgatctacg gctaccaggg cctcaacgtc gaggcacaga
     2881 tgcgcacctc gacgtccctg ctgcactgga cacggcggat gatcgacacc cgcaagcgcc
     2941 atccgacctt cggcgccggc aatttcgccg aactgggtgc gtcgaatccg agcatcctgg
     3001 cgttcgtccg cgagttcggc gacgaccggg ttctgtgcgt gaacaacctg tcgcgcttcc
     3061 cgcagccagt ggaactcgac ctacgccggt acgagggtgt ggttccggtg gagatgctgg
     3121 ggggtatgca cttcccgaag gtgggcgagc tgccctacct gctgaccctg ccgggccacg
     3181 gcttctactg gttcacgctg ccggccaagc gcgcggagga tgtctaaatg agcaccaaga
     3241 aggtagaccg gacgcagctc gaggacttcc tgcgcgactg gctaccgaag cagcgctggt
     3301 tcgccggcaa agaccgcggc gcgaaagcgg tgcatatcgc gactgagcgg gtgttgctcg
     3361 ccggcgaccc gtcccttttg cacctgtttg tgcgagtcga acaaaacccg gccgtgaacc
     3421 cgctccctga cctcgagccc gccggcgaca gcgtcacgac cacgtatcag ttgctgctcg
     3481 gtgtccgctc caccctgccc ccggagctcg agcacgccag catcggccag gtcgtcgatc
     3541 tgggttgggc ctacgaggcc agccaggacc atgatctgat gcacacgatg ctgggctggg
     3601 ttgcgggcga ggagacccgt ggcgacatcg tcttcgccct tgaaccgggc gcgaagatcg
     3661 atcccgacgc cgtcagcctg gtgcttggcg gcgagcagtc gaacacgtcg gtggtcttcg
     3721 gcgagcagac catcatgaag ctcttccgca tcgtcgttcc gggcctcaat cccgacctcg
     3781 aggtgacgcg tgcgctcacc gagatgggca acctgcacgt cccggcgctg caaggctgga
     3841 tcgaaagcga cgtcgaaggc accgccagca cgctaggcct gctccaggaa ttcctgcgga
     3901 ccggctccga tggctgggcg ctcgcgctga ctagcgtgcg cgatctgttc gccgagggtg
     3961 acctgcacgc tggcgaggtc ggcggcgact tcgcagcaga gtcggagcga ctcggcatgg
     4021 ccacggccga cgtgcactcg gcgatggcac aggctctgcc gacggggatc gctgaccagc
     4081 acgctcttga aacgatggcc cagtcgatgc gcaagcgact cgacgcggca cggatcgccg
     4141 tcccggaact tgccgatcac gtcgtggcaa tctctgcggt ctttgacgct ctcgcgaaca
     4201 gccgcgaacc ggtcaccctg caacgcgtcc acggcgactt ccacctcggc caggtcatgc
     4261 gcacggacaa tggctgggtg cttctcgact tcgagggtga gcccggcgcc ccgatcgccg
     4321 cgcggaccgc actctcgagc ccgttgcgag atgtcgccgg catgttgcgg tcgttcgact
     4381 acgcgtcgcg ttacctgctc gccgaccaac cagcggccgc gcagctcgaa tatcgggccg
     4441 ccgagtgggc agagcgcaac cgcgacgcgt tctgcaccgg ctacgcgaag agtgccggcc
     4501 gcgacccgcg ggaggacgat gtgttgctgc gtgctttcga actcgacaag gcggtgtacg
     4561 aggtggcgta cgaagctcga aaccgtccca gctgggtacg gattccgctg ggttcgatcg
     4621 agcggctggt ggcggcatga ccagcggacc tatgcctccg aagaaggacg agccagaagc
     4681 tccagtgacc gcgcagcccg cggcaaccgt cgtccctgag aagaaggtcg ccacagtgac
     4741 gaagccagcc gacaccaagc cagccgacac gaggctagcg agcgttccgc gtgcgccggt
     4801 gacggcgcca ccggaggaga tcgaccgctt ggtctccgga gcgcatcaca atccgcacgg
     4861 cattctcggc gcgcaccttg ccggcggcgt cgtgactttc cgcagcttcc ggccgaacgc
     4921 gcgtgaggtc accgtgttgg tcgacctgct ccgtattccg ctcgcgcacg aacgtggcgg
     4981 ggtgtggagc gcgacgtgga ccagttcagc cgttcccgat taccgcgtcg aggtcgtcta
     5041 cgacgacggg atcccgcaca ttgtcgacga gccgtaccgc tttctgccga cgctcggcga
     5101 gatcgacctg cacctcatcg gtgagggtcg ccatgagcag ttgtgggagg tgctcggcgc
     5161 gcacgagcac cactacgaca cgccgagtgg cgtggtgaat ggcacgtcgt tcgccgtctg
     5221 ggcaccgaac gcgcgcgcaa tgcgggtcgc cggcgacttc aacttctggg actcccaagc
     5281 caacccgatg cgatccctgg gttccagcgg cgtgtgggag ctgttcatcc ctggcgtcgc
     5341 gtccggcgac cgctacaagc tgaacgtgct cgggtccgac ggccagtggc gcgagaaggc
     5401 cgacccgatg gccttcgcga c
//
LOCUS       DATCBJ010000062         2319 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_70479, whole genome shotgun sequence.
ACCESSION   DATCBJ010000062 DATCBJ010000000
VERSION     DATCBJ010000062.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2319)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2319)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2319
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_70479"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..253
                     /locus_tag="VII50_01390"
     CDS             <1..253
                     /locus_tag="VII50_01390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HEY5049523.1"
                     /translation="TFRSLTADCTATVEIVARFLALLDLFRDGCIAFEQVTALGDLLV
                     RWTLQDDADDSESAAGIIGEPADADARIALDPTPSTVDD"
     gene            298..909
                     /gene="scpB"
                     /locus_tag="VII50_01395"
     CDS             298..909
                     /gene="scpB"
                     /locus_tag="VII50_01395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HEY5049524.1"
                     /translation="MTIEFEAEDSVPEHGHLTGLQLRGAIEAILLIVDEPADPTVLAQ
                     VLTTPEPEIVAELQALQGNYDETGRGFELREIAGGWRLYTRAEFAPFVERFVLEGQQA
                     RLTQAALETLAVVAYRQPVSRTRVSAVRGVNVDGVMRTLVSRGLVEEAGADHESGAIL
                     YQTSRYFLERLGLGNLTELPNLADFLPESTTIDALEDELSSEG"
     gene            911..1657
                     /locus_tag="VII50_01400"
     CDS             911..1657
                     /locus_tag="VII50_01400"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003796661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HEY5049525.1"
                     /translation="MNSSDIGQSEIRLQKVLAAAGLGSRRACEEMIAQGRVEVDGQLV
                     LEQGKRVDPENAVIKVDGMRVTTRTSAVYLALNKPRGMVSTMSDPQGRPCLADLVGNR
                     EQRLFHVGRLDADSEGLILLTNDGELGHRLTHPSFGVPKTYLAEVVGAVPRDLGRTLK
                     TGVELDDGLVQVDSFRVVGRSGARVMVEVVLHEGRNHVVRRLLAAVDHPVQRLIRTAV
                     GPVRLGDLRPGRSRPLNSHEIAELFAAVDL"
     gene            2034..>2319
                     /gene="aroH"
                     /locus_tag="VII50_01405"
     CDS             2034..>2319
                     /gene="aroH"
                     /locus_tag="VII50_01405"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR01796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004106 - chorismate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HEY5049526.1"
                     /translation="MAVRAVRGAIQVDADEPDLIFQGVRDMLAAVLARNELGSQDVIS
                     IFFTATTDLTSCFPAAGAREVGFSDVPLLCASEIAVPGSMPRTLRLLAHVE"
BASE COUNT          385 a          714 c          785 g          435 t
ORIGIN      
        1 gacattccgg tcgcttacgg ccgactgcac agcgacggtg gagatcgtcg cccgattcct
       61 ggcgctgctg gacctcttcc gtgacggttg cattgccttc gagcaggtga ccgcgcttgg
      121 ggatctcttg gtccgctgga cgctgcagga cgacgccgat gacagcgaga gcgccgcggg
      181 catcatcggc gagccggctg acgccgatgc ccgtattgcg ctcgatccga caccgtcgac
      241 cgtggacgac tagtgtcgcc tgccggggat cgaccgagcg ggacgaaggc aggtgcgatg
      301 acgatcgagt tcgaggccga agacagcgtg cctgagcacg gccacctcac cgggctgcag
      361 ctgcggggcg cgatcgaggc gatcctcttg atcgtcgacg agccggcgga cccgactgtt
      421 ctcgcccagg tgctgaccac ccccgagccc gagatcgtcg ccgagttgca ggcgctccaa
      481 ggcaactacg acgagaccgg gcgcggcttt gagctgcgag aaatcgcggg cggctggcgt
      541 ctctatacgc gtgcggagtt cgcgcccttt gtcgagcggt tcgtcctgga ggggcagcag
      601 gcgcggctca ctcaggccgc actggaaacg ctcgccgtgg tcgcctaccg acagccggtc
      661 agccgcacga gggtcagcgc ggtgcgggga gtcaacgtag acggggtgat gcggacgctg
      721 gtcagccggg gactcgtcga agaggccggc gccgatcacg agtcgggcgc gatcctctac
      781 cagacgtctc ggtacttcct cgagcgccta ggcctcggca acttgacgga actaccaaat
      841 ttggctgact ttctgcccga gtctacgacg attgatgcac ttgaagacga actctcaagt
      901 gaaggttgag atgaatagct cggatatcgg ccagtcagag attcgcctcc agaaggttct
      961 ggccgcagct ggcttgggca gtcggcgagc ctgcgaggag atgatcgccc agggccgggt
     1021 cgaggtcgat ggccagctgg ttctcgagca gggcaagcgc gtcgatccgg agaacgcggt
     1081 gatcaaggtc gacgggatgc gcgtgacgac ccgtacatcc gccgtgtacc tcgccttgaa
     1141 caagccgcgc ggaatggtca gcaccatgtc ggacccgcag ggccgcccat gcctggccga
     1201 tctggtcggt aatcgtgagc aacggctctt ccacgtgggc cgcctggacg cggacagtga
     1261 gggcctgatc ctgctgacca acgacggcga gctcggccac cggctgacgc atccgtcgtt
     1321 cggcgtaccg aagacctacc tggccgaggt cgtgggcgcg gttccccgtg atctgggcag
     1381 gacgttgaag accggcgttg agctggacga cgggttggtc caggtcgatt cgtttcgggt
     1441 ggttggccga tccggcgccc gggtgatggt cgaagtggtc ctgcacgaag ggcgcaatca
     1501 cgtcgtccgg cggttgctcg ctgcggtcga tcatcctgtc cagcggctga tcaggacggc
     1561 ggtcggaccg gttcggctgg gggatctgcg ccccgggcgc agccggccgt tgaacagcca
     1621 cgagattgcc gagctgttcg ccgccgtcga tctttaggac ggcttttggt tggtggcgat
     1681 cttggagtcc agtagtcgtc gtcgatccaa agcgcgatat cgagatgtcg ataagtagtt
     1741 tggattatct gtcggcccgt accgaaactg cagggcggcg ttcccgacga tcgcgtacga
     1801 gcgaccatcc cctattcatt tgcacagctc acagcgccta tggagtcgac ttattgctaa
     1861 gtcgtccacg cccgatcccg ggtcgtctga cccctgcgta gtcgccgaat acggccatcg
     1921 ctttgtcgat agggcggcat cgcgactgac cgacacaccg ctctggcgct ttgaggcgat
     1981 gtcactagct cgctatggtg acctcctggt cggccaacgt aggaggagca accgtggcgg
     2041 ttcgcgctgt tcgaggcgcc atccaggttg atgccgacga gccagacttg atcttccagg
     2101 gggtgcgcga catgttggct gccgtcctgg cgcgcaacga gttgggctcg caggatgtca
     2161 tcagcatctt cttcaccgcg accaccgatc tgacctcctg ctttcctgcc gccggcgcca
     2221 gggaagtggg gttctccgat gttcccttgc tctgcgcgag cgagatcgcg gtccccgggt
     2281 cgatgccacg caccctgcgg ctgctcgccc acgtcgaga
//
LOCUS       DATCBJ010000063         2326 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_545465, whole genome shotgun sequence.
ACCESSION   DATCBJ010000063 DATCBJ010000000
VERSION     DATCBJ010000063.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2326)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2326)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2326
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_545465"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..446)
                     /locus_tag="VII50_01410"
     CDS             complement(<1..446)
                     /locus_tag="VII50_01410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY5049527.1"
                     /translation="MPELPSRVDVVVVGAGHNGLVAATFLARSGLDVVVVEAAQKLGG
                     AAKTEQPFARVPGLSQSTGAYLLGLMPPELPRKLGVEFPVLRRDPHYFLPTTGDRYLL
                     FGSDREATRRQYEDFFSAADWKADQALQAELDLLRADLAPAWLAEPL"
     gene            complement(455..1042)
                     /locus_tag="VII50_01415"
     CDS             complement(455..1042)
                     /locus_tag="VII50_01415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5049528.1"
                     /translation="MTEGQDVDPIDTEHADPAPIASLSLDDVVLPIYDLQAFNDADLH
                     RVSSFVTNFGSWHLPPEVATALAAGKNSAALEWIHDTGELVLLGGTPTEGELSAELPG
                     FAGAAAEFTPAFLGGEAGATKTDGSGMVREYFKGEVMPPGSRVALMAHIEHGPAVHEL
                     LWGWHHEHRTAEGWNWLVERLARLEDASGQSDHLG"
     gene            complement(1094..1840)
                     /gene="dapB"
                     /locus_tag="VII50_01420"
     CDS             complement(1094..1840)
                     /gene="dapB"
                     /locus_tag="VII50_01420"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HEY5049529.1"
                     /translation="MNNAVAVLGADGKMGATVCAAVRAATDLELVGSFDVGYSHDELA
                     ASGAQVVVDFTTPAAVMGNIEFCVGEGMHVVVGTTGFDADRLATVAAWLKAAPNVGVV
                     IAPNFGIGAVLTMAFAEKAARYFESAEIVELHHPYKADAPSGTSRHTAERIAAAWRDA
                     GLAPIPDATTSGLDGARGADVDGVHVHSVRLGGLVAHQEVLFGGHGETLTLRHDSLDR
                     VSFMPGVLLAVRSVAARPGLTVGLESLLDL"
     gene            complement(1848..>2326)
                     /locus_tag="VII50_01425"
     CDS             complement(1848..>2326)
                     /locus_tag="VII50_01425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HEY5049530.1"
                     /translation="LNGALGGGMSSRLFQEIREKRGLAYSVYSYAAHHAETGMFGVYA
                     GCQPAKAAEVLDICRDQLDLVARAGITTEELSRGKGQLAGALVLGLEDTGSRMSRLGK
                     AELVYGELLTVDELLARIDSVTLEQVSDVAATVLSAKQTLAVIGPFDDDAFADAVA"
BASE COUNT          434 a          779 c          746 g          367 t
ORIGIN      
        1 aggggctcgg cgagccacgc aggtgcgagg tcggcgcgca gcagatcgag ttcggcctgc
       61 agcgcttgat ctgccttcca gtcagcagcg gagaagaagt cttcgtactg ccgtcgcgtc
      121 gcctcgcgat cagagccgaa caacaggtac cgatcgccgg tggtcggcag aaagtagtgc
      181 ggatcacgac gcagcactgg aaactcgacg ccgagcttgc gcggcagctc gggcggcatg
      241 agtccgagta gataggcacc agtcgactgc gaaagccctg ggacgcgcgc aaacggctgc
      301 tccgttttcg ctgcaccgcc aagcttttgc gccgcctcga cgacgacaac gtcgagcccg
      361 ctgcgtgcga gaaaggtcgc cgcgacaagt ccgttgtggc cagcgccgac aacgacgacg
      421 tcaacccgcg aaggtaactc cggcatcggg tacgtcagcc gaggtggtcg gactggcccg
      481 acgcgtcctc gagccgcgcg agccgttcga ccagccagtt ccagccttca gcggtgcggt
      541 gctcgtgatg ccatccccac agcagttcgt ggacggcggg accgtgctcg atgtgagcca
      601 tgagggccac ccgggaaccc gggggcatga cctcgccctt gaagtactcg cgcaccatgc
      661 cggagccatc ggtcttcgtc gcgcctgctt cgccacccag gaaggccggc gtgaattccg
      721 ctgccgcgcc ggcaaaacca ggtagttcgg cggagagctc accctctgtc ggggtacccc
      781 cgagcaacac gagttcgccg gtgtcatgga tccactcgag cgcggcggag ttcttgcctg
      841 cggccaaggc cgtggcgacc tcagggggta gatgccagga accgaagttc gtcacgaagc
      901 tgctcacccg gtgcagatcg gcgtcgttga acgcctgcag gtcgtagatc ggcagcacga
      961 cgtcgtcaag cgacagcgat gcaatcggcg ccggatcagc gtgctcggtg tcgatcggat
     1021 cgacgtcctg gccttcggtc atcgcgtgcc tctctgcggc atcctccgac agcggaggta
     1081 catacgggtg gaactacaga tcgagcaggc tttcaagacc gacggtcagg ccgggacgcg
     1141 ccgcgacgct tctcaccgcg agcagcactc ccggcatgaa cgagactcga tcgagtgaat
     1201 cgtggcgaag ggtcagcgtc tcgccgtgcc caccgaacag cacttcctgg tgagcgacaa
     1261 gtccgccgag ccgcaccgag tgaacgtgca cgccgtcgac atcggcgcca cgagctccat
     1321 caaggccgga cgtcgtagcg tcgggaatcg gcgcaaggcc ggcgtcgcgc catgcggccg
     1381 caatgcgttc cgcggtgtgc cgcgacgtcc cggagggcgc atctgccttg tacggatgat
     1441 gaagttcgac gatctcggcg gattcgaagt agcgcgcagc cttctcggca aacgccatcg
     1501 tgagcaccgc accgattccg aagttggggg cgatcacgac gccgacattc ggagccgctt
     1561 tcagccacgc cgcaaccgtc gcgagtcgat cggcgtcgaa gcccgtcgta ccgacgacga
     1621 catgcatgcc ctcgccgacg cagaactcga tattgcccat caccgcagcc ggcgtggtga
     1681 agtcgacgac gacctgcgca ccggatgcgg caagctcgtc gtgggagtag ccgacgtcga
     1741 acgaccccac caactcaaga tcggtcgcag cgcggacggc ggcgcagacc gtcgcgccca
     1801 tctttccgtc agccccaaga actgcgaccg cgttattcat cgcgcgctca cgctacggcg
     1861 tccgcaaatg cgtcgtcatc gaacggtccg atgacagcga gcgtctgctt tgccgacaac
     1921 acggtggcag cgacatcgct gacctgctca agggtcaccg aatcgatgcg cgcgagcagt
     1981 tcgtcgaccg tgagcaactc gccgtagacg agttcggcct ttccgagccg actcatgcgc
     2041 gagccggtgt cttccaggcc gagaacgagc gcccccgcga gctgtccctt cccgcgcgac
     2101 agctcctcgg tggtgatgcc cgcgcgggcc acgagatcga gctgatcgcg gcagatgtcg
     2161 agcacctccg ccgccttcgc gggctgacaa ccggcgtaca caccgaacat gccggtctcg
     2221 gcgtgatgtg ccgcgtagct gtaaacggag taggcgagtc cgcgcttctc acggatctcc
     2281 tgaaacaacc gcgagctcat gccgccgcca agtgcaccgt tcagca
//
LOCUS       DATCBJ010000064         6211 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_360083, whole genome shotgun sequence.
ACCESSION   DATCBJ010000064 DATCBJ010000000
VERSION     DATCBJ010000064.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 6211)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6211)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6211
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_360083"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..932
                     /locus_tag="VII50_01430"
     CDS             <1..932
                     /locus_tag="VII50_01430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aldo/keto reductase family protein"
                     /protein_id="HEY5049531.1"
                     /translation="GSQVEGDAAKRCVRAALDAGITTFDTADVYAGTRAESVLGDALA
                     GTRRESYELCTKVFFATGEGHNDRGLSRKHIIESCHASLRRLQTDHLDLYQAHRFDVE
                     TPLEETLRAFDDLVRQGKVNYIGVSEWTADQIASALRIADDMGLDRIVSNQPQYNMLW
                     RVIEAEVVPLSQREGIGQIVFSPLAQGVLTGKYKPGAEPPEGSRATDTKGGARFVSRL
                     LEDAVLSRVQLLQPLAAQAGLPLSQLALAWVLHNPNVSSAIIGATRPEQINQNVAASG
                     VKLEADLLARIDEILDPIIERDPRKTERLAKRP"
     gene            complement(929..1099)
                     /locus_tag="VII50_01435"
     CDS             complement(929..1099)
                     /locus_tag="VII50_01435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5049532.1"
                     /translation="MAPADLPPAETFPTQSDAESWLGEEWRPLLAAGVARVSLLEGER
                     VVYGPMSLDPAS"
     regulatory      1200..1383
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            1768..2925
                     /locus_tag="VII50_01440"
     CDS             1768..2925
                     /locus_tag="VII50_01440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0009986 - cell surface [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEY5049533.1"
                     /translation="MAPTNVGLFGAQDPTYDGVYRQSLAILGLVATGHVPNNPAVGWL
                     LAQQCSDGAFTAYRANTSAACTPASEDENATAAAVEALVAVVRPTATAVAALKHFQLA
                     DGGFYDNTAFGPAASDANSTGLALSALVAAGVNPATVTSTTGKTGDDYLRSVQIACTA
                     TTGAGAYDVQGEATLHANDYATVQALLGELGKAFPLSPATSTGSTPTCPTTVADSATS
                     AAAASSYLAARLAATKGAIPSAFGSGTDWTTTANAVLDLEAGGAGTTAIAPALTALEA
                     GAKAYVKTAGAFAPGPLATLLMVAHTSGVDPTAFGGVDLAAALASTERVAAAAPPAAE
                     PSRSPIATPVATLPTTGSTDALPLGALGALLVGSGIAALLVGRRRVGQHEC"
     gene            2930..3796
                     /locus_tag="VII50_01445"
     CDS             2930..3796
                     /locus_tag="VII50_01445"
                     /inference="COORDINATES: protein motif:HMM:NF040672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO2322 family protein"
                     /protein_id="HEY5049534.1"
                     /translation="MTMTRAAALGLAALAGYAAVVITVPGQARAGTADRYWAYYVAHG
                     ASWQYSQRGPATEHPVDGEVQGWRFAVQVDAANGLLPRAAPAFATLCASTPAEAGELR
                     VGVVIDFGLATDAPAHERPPAAVVPGCVYVHDGGTGADVLAAAAAVRIGTGSDAGLVC
                     GIDGYPRTECAVAVATRSTAPAPPTQTPAPASPRVAPSTRPPTAATPASSRAAAPTAA
                     GQSASVPAPETLAAASSGSARPATASALSLAALRSSTRRGHGIQAITVVGIGLIVVLG
                     AGALWRTRVRGR"
     gene            3801..4949
                     /locus_tag="VII50_01450"
     CDS             3801..4949
                     /locus_tag="VII50_01450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009084757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CbiQ family ECF transporter T component"
                     /protein_id="HEY5049535.1"
                     /translation="MSLYTGRPARALHPGAWWAWSLALATAATRTTNPIVLLLLVAVA
                     AFTVSARRSPQSSRSFGLFLRLGLLVLAVRVVFDIVFGAQPTGHVLVRVPTVPLPGWA
                     AGLHLGGNITVQGMLTAVYGGLQLATLLVCVGSANALASPRRLLRAMPGALYEAGVAI
                     TVAMSFAPQLAAASVRVRRARRLRGRPASGVRSWLGIALPVLEDALERSIELAAAMDS
                     RGFGRRGAASALRRRLVSSAVVVGLVAVAIAVYELLDATSPTLIGLPLLVVGTVIACC
                     AVLFGGRQPTRTKYRPDSWQVPEWIVIASGVAALAGMLVAGRLYPGSLYTTTYPLVVP
                     QLPWPALVGLVLALIPAWVTSPKTPALQGFSTTLEPAQDSPTPAGVGR"
     gene            4946..>6211
                     /locus_tag="VII50_01455"
     CDS             4946..>6211
                     /locus_tag="VII50_01455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY5049536.1"
                     /translation="MIRFESVTITYPDAEGPAIRDVELSIPEGELALVIGRTGAGKST
                     LLKAINGLVPHFTGGTLAGRVTVDGRDTRSYPPRELADVVGYVGQDPLAGFVTDSVEE
                     ELAYAMESLGVSPAVMRRRVEDILDSLGLTGLRQRPLRTLSSGEQQRVAIGSVLTAGA
                     RVLVLDEPTSALDPPAADDVLSALTRLVHDLGVTVVAAEHRLERVIQYADRVVHVDDD
                     GRVHDTTPIAAMRTSVVAPPVVELGRLVWWAPLPLSVRDARRAADTLRAQLATMPTPH
                     DRPAATGEPLIEVRKLHANYDDIIALRGIDLVVRPGEIVALMGRNGAGKSTLLAQLVG
                     LNRPLAGRVVVAGHDPYRRRGSDLVRDVGLVPQEPADLFWADTVDDECAGAETGARAL
                     LDRLAPGIAGHTHPRDLSEGQRLALALALV"
BASE COUNT          928 a         2195 c         2090 g          998 t
ORIGIN      
        1 acgggtcaca agtcgaggga gacgccgcga agcgctgcgt tcgagccgca ctcgacgcgg
       61 gcatcacgac attcgacacc gccgacgtct acgccggaac gagggcggag tccgtcctgg
      121 gcgatgcgct cgcgggcacc cgtcgggagt cctacgagct gtgcaccaag gttttcttcg
      181 ccacgggcga gggtcacaac gaccgcggct tgtcgcgcaa gcacatcatc gagagctgcc
      241 acgcgtctct tcgacgactg cagaccgacc atctcgacct ctaccaagcg caccgtttcg
      301 atgtcgaaac gccgctcgag gagacgttgc gcgccttcga cgatctggtg cgacagggca
      361 aggtcaacta catcggcgtg agtgagtgga cggcggacca gatcgcgtct gccttgcgca
      421 tcgccgacga catgggtctc gaccggatcg tgtcgaacca gccgcagtac aacatgctct
      481 ggcgggtcat cgaggccgaa gtcgtcccac tttcacagcg cgaaggcatc ggccagatcg
      541 ttttctcgcc gctggcacaa ggagtgctca cggggaagta caagccaggc gccgaaccgc
      601 cggagggatc gcgagccacc gacaccaagg gcggcgcgag attcgtctcc cgcctgctag
      661 aagacgcggt gttgtctcgc gtgcagctcc tgcagccgct cgctgcgcag gccgggctcc
      721 cgctgtcgca gctcgcgctt gcgtgggtgt tgcacaaccc gaacgtgtcg agcgcgatca
      781 tcggggcgac caggcccgag caaatcaacc agaacgtggc ggcgtcgggg gtcaagctgg
      841 aagccgacct gctcgcgcgc atcgatgaga tcctcgatcc gatcatcgag cgtgatccgc
      901 gcaagacgga gcggttggcg aaacggccct agctcgcagg gtcgaggctc atcggcccgt
      961 aaacgacccg ttcgccttcg agcaggctga cccgagcgac gcccgccgca agcagcggcc
     1021 gccattcctc gccgagccac gactcggcgt ccgactgcgt cgggaaggtt tccgcgggcg
     1081 gcaggtcggc gggtgccacg ggttcaccgt cagcattctc gtaccgccac gaccagctca
     1141 ccatgcgctc ctccggtcaa ctcgcgtcat cgattcgacc ggtaggcagg ctagcgcgtc
     1201 ctttgactgg gtgccccggc tcgtggtttg ctcgcgccac aactgaagac gcatccgcgt
     1261 tcaggggaag ccggtccaat gccggcgctg acccgcaacc gtaggccgca ccgatgcccg
     1321 tttcttttgt cttgggcacc ggtacggcaa gccggattac ctgccggatg cgaaacggct
     1381 ccaatcgcct gccgaggttc gcggggcgga gcccgggtcg caccgacccg ccaacgcccc
     1441 cacgctgcgc cgtttgcgct cgtggggttc gtgttgtcgg gggcggtcga gggccgggac
     1501 ctaccacgtg gaccccggcc acctcccgag aggaacacga tgtcgcacgg atccacgcat
     1561 ctcacggagc cacagcgtct ccaggacaca cagcgtctcc aggacacaca gcgtctccag
     1621 gaaatgccgc gtcatggggc cgggtctgcc ctccgaacgc ttgctgcatg tgccgtagga
     1681 gccgtcgccg tcaccacgct cctcgcctcg accgcgatgg ccgccacgcc ggccgccaca
     1741 cccggaactg ccacccctat cgccatcgtg gcaccaacca atgtcggcct gttcggtgcg
     1801 caagatccga cctacgacgg tgtctaccgg caaagcctgg cgatcctcgg cctcgtggcg
     1861 accggtcacg tccccaacaa ccctgcggtc ggatggttgc ttgcgcagca atgctccgac
     1921 ggcgcgttca cggcataccg cgcgaacacc tcggcggcgt gcacgccagc ctcggaggac
     1981 gaaaacgcaa ccgcggccgc ggtcgaagcc ttggtcgccg ttgttcggcc gacagcgacg
     2041 gccgtggcgg ctctcaagca tttccaattg gcggacggcg ggttctacga caacaccgcg
     2101 ttcggccccg cggcatcgga tgcaaactcc accggcctgg cgcttagtgc cctggttgct
     2161 gcgggcgtga atccggccac agtcacctcg acgaccggca agaccggcga cgattacctg
     2221 cggtcggtgc agatcgcgtg caccgcgacg accggcgcgg gtgcctatga cgtccagggt
     2281 gaggccaccc tgcatgccaa cgactacgcg actgtgcagg cgttgctggg cgaactcggt
     2341 aaggcgtttc cgctgagccc ggcaacgtcg accggatcga cacccacctg ccccaccacg
     2401 gttgccgact cggcaacgtc ggccgcggct gccagcagct accttgccgc gcggttggcg
     2461 gcgacgaagg gggcgatccc ctcagcgttc ggcagcggca ctgattggac gacgacagcg
     2521 aacgccgtgc tcgaccttga ggccggtggc gcgggaacaa cggcaatcgc accggcactg
     2581 accgcactgg aagcaggcgc caaggcgtac gtgaagacag cgggcgcatt cgcgcccgga
     2641 ccgctggcaa ccctgctgat ggtcgcccac acgagcggtg tcgaccccac tgcgttcggc
     2701 ggtgtcgacc tcgcggccgc gctggcatct accgagcggg tcgctgcggc agcgccaccg
     2761 gctgcggagc cgtcccgatc gccgattgcg acgccggtcg ccaccctgcc cacgaccggt
     2821 tcgaccgacg ctctaccgct cggcgcgctc ggcgcgttgc tggtcgggtc ggggatcgcc
     2881 gcgctgctcg tcggccggcg ccgggtcggt cagcacgagt gctgaccaca tgacgatgac
     2941 cagggcggcc gctctcgggc tggccgcctt ggcgggttac gccgccgtcg tcatcaccgt
     3001 tcctggtcag gcacgtgcag gaacggccga ccggtactgg gcgtattacg tcgcgcacgg
     3061 cgcgagctgg cagtactcgc agcgcgggcc tgcgactgag cacccagtcg atggcgaggt
     3121 tcagggatgg cggttcgcag tgcaggttga cgcggcgaac gggctgctgc ccagggccgc
     3181 gccagccttc gccacactct gcgcgtcgac cccggccgag gccggcgagc tacgtgtcgg
     3241 cgtggtcatc gacttcgggc tcgcgaccga cgcacctgct cacgagcgac cgccggcggc
     3301 ggtcgtgccc ggatgcgtgt atgtgcacga cggcggaacc ggcgcggacg tcctggcggc
     3361 tgcagccgcc gtccggatcg gcaccgggtc ggacgccgga ctcgtgtgcg gcattgacgg
     3421 atatccgagg accgagtgcg cggtcgccgt cgcgacccgc agtacagcac cagcgccacc
     3481 cacgcagacc cctgctcccg ccagcccgcg ggtcgcaccg agtacccgcc cgcccaccgc
     3541 ggcgacgccg gcttcgagcc gcgccgcagc gcccacggcc gccgggcagt cggcgtccgt
     3601 tcctgcgccc gagacccttg cagcggcgtc atccgggtcg gcccgaccgg cgacggcgtc
     3661 ggcactctcg ttggcagcgt tgcgatcgag cacccgccgc ggccatggca tccaggcgat
     3721 caccgtcgtg gggatcggcc tcatcgtggt gctgggcgcc ggagcgctct ggcgtactcg
     3781 cgtgcgcggg cggtgagcag atgagcctgt acaccggccg gccggcccgc gccttacacc
     3841 caggggcatg gtgggcatgg tcacttgcgc tggccacggc cgcgacgcgc accacgaacc
     3901 cgatcgtcct gctactgctc gttgcggtcg ccgcgttcac cgtgtcggca cgccggtcac
     3961 cgcaaagctc ccgatcgttt gggctcttcc ttcgcctcgg cctcctcgtg ctcgccgtcc
     4021 gcgttgtctt tgacatcgtg ttcggcgcac aacccaccgg tcacgtgctg gtgcgggtgc
     4081 cgactgtccc attgccgggg tgggcagctg gattgcatct cggcgggaac atcaccgtgc
     4141 agggcatgct cacggcggtc tacggcggcc tgcagctcgc gacgctgctc gtctgtgtcg
     4201 gatcggcgaa cgcactggcc agcccgcgcc ggctccttcg agcgatgccg ggcgcgctgt
     4261 acgaagcggg cgtcgcgatc acggtcgcca tgtcattcgc tccccagctc gccgccgcgt
     4321 cggttcgggt gcggcgggcc cgacgactgc gcgggcgtcc agcttccggc gtccggtcct
     4381 ggctcggtat tgcactcccc gtcttggagg acgctctgga gcggtcgatc gagttggcag
     4441 cggcgatgga ttcgcgcggg ttcggcaggc gcggggccgc gtctgcgcta cggcgccgac
     4501 tggtctcgtc cgcggtcgtc gtcggactcg tcgcagtcgc catcgcggtc tacgaactcc
     4561 tcgacgcgac atcaccgacg ttgatcgggc tcccgctgct tgtcgttggc acggtcatcg
     4621 cctgctgcgc tgttttattt gggggccgcc agcccacccg gacgaagtac cggccagatt
     4681 cctggcaggt ccccgagtgg atcgtgatcg cgtcgggcgt tgcggcgctg gccggcatgc
     4741 tcgttgcggg ccgcctgtat cccgggtcgc tgtacacgac gacatatcca ctggtcgtcc
     4801 cgcaattgcc gtggcccgcc ttggtcgggc ttgtgctcgc gctgatcccg gcgtgggtca
     4861 catcgccgaa aacccctgcc ctgcaagggt tttccacgac tttggagccg gcgcaggact
     4921 cccccacgcc agcgggagtc ggccgatgat tcggttcgag tcggtcacca ttacctaccc
     4981 ggacgccgag gggccggcga ttcgcgacgt cgaactgtcg atccccgaag gcgaactcgc
     5041 gttggtcatc ggccgcaccg gcgcgggcaa gtcgacgttg ctgaaagcca tcaacgggct
     5101 ggtgccgcac ttcaccggcg gcacactggc gggccgcgtc accgtcgacg ggcgcgacac
     5161 tcgcagctat ccgccgcgcg aactcgccga cgtcgtgggg tacgtcggtc aggatccgct
     5221 tgccggtttc gtcaccgaca gcgtcgaaga agaactcgcc tacgcgatgg aatcactggg
     5281 cgtatcgcct gcggtgatgc gccgtcgcgt cgaggacatc ctggactccc tgggccttac
     5341 cggtctgcgc cagcgaccac tgcgcacgct gtcgtcaggc gagcaacagc gagttgctat
     5401 cgggtcggtg ctcacggcag gcgcgcgggt tcttgtgctc gacgagccga cgtcggcact
     5461 cgaccctccc gcggccgacg acgtcctgtc agccttgacg cggctcgtgc acgatctggg
     5521 ggtcacggtg gtggccgccg agcaccggct cgagcgggtg atccagtacg ccgaccgagt
     5581 cgtgcacgtc gatgacgacg gtcgcgtgca tgacacaacc ccgatcgcgg caatgcgaac
     5641 cagtgtggtc gcgccgcctg ttgtcgagct cggccggctc gtgtggtggg cgccgttgcc
     5701 gctgtcggta cgcgacgcac gccgagcggc cgacaccttg cgtgcccagc tggccacgat
     5761 gcccacacca cacgaccggc ctgcggccac tggtgagccg ctgatcgaag tgcgcaaact
     5821 gcacgcgaac tacgacgaca tcatcgcgct gcgtggcatc gacctcgtcg tgcggccggg
     5881 cgagatcgtc gcgctgatgg gccgcaacgg cgccggcaag tcgacgctgc tcgcgcagtt
     5941 ggtcggactg aaccgtccgc ttgcgggccg ggtggtcgtc gcgggccacg atccgtaccg
     6001 gcggcgtggc tccgatctgg ttcgcgacgt cggcctggtc ccacaggagc ctgccgacct
     6061 cttttgggcc gacacggtcg acgacgaatg cgccggcgcc gaaaccggtg cgcgggcact
     6121 gctcgaccga ctcgcgcccg gcatcgccgg ccacacgcac ccgcgggatc tctccgaagg
     6181 acagcggctt gcgctcgctc tcgcactggt c
//
LOCUS       DATCBJ010000065         2893 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_359955, whole genome shotgun sequence.
ACCESSION   DATCBJ010000065 DATCBJ010000000
VERSION     DATCBJ010000065.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2893)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2893)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2893
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_359955"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1185
                     /locus_tag="VII50_01460"
     CDS             <1..1185
                     /locus_tag="VII50_01460"
                     /inference="COORDINATES: protein motif:HMM:NF020926.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-binding domain-containing protein"
                     /protein_id="HEY5049537.1"
                     /translation="LVARDDPLDTYLVHHPDALFGPPVEAAVFDPDNPYVLGPHLCAA
                     ASEWPLSPDDLDLFGPTATDVVADLVDRRLLRARPNGWFWTKRERACDLADIRGTGGA
                     PVRIVDEATGQLLGTANAAAADSTVHTGAVYLHQGETHVVRTFDVDDRVALVEPADVD
                     YFTSARDITDVRITATSRTTLWGAAEVSFGSVDVTTRVVSFLRRRVVSGEVLGEQALD
                     LPERQLSTRAVWWTIGEQDLARAGFVWADLAGAAHAAEHASIGLLPLVATCDRWDIGG
                     VSTALHEDTGRLTVVVYDGYPGGAGFAERGYEAAAIWLTATREAIAACACETGCPSCV
                     QSPKCGNGNDPLDKAGAIRLLDTLLSDAPLPDALSDAPLSDLPLPDRPSAITLALPAH
                     AR"
     gene            complement(1844..>2893)
                     /gene="ssd"
                     /locus_tag="VII50_01465"
     CDS             complement(1844..>2893)
                     /gene="ssd"
                     /locus_tag="VII50_01465"
                     /inference="COORDINATES: protein motif:HMM:TIGR03815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein Ssd"
                     /protein_id="HEY5049538.1"
                     /translation="AGARLGWTKAALVLVGDDVSITLGRASVRRRVGVVLVGRDLDDG
                     TVWKRAVGVGAEHVALLPDAEPWLIARMAAAVDESAALGLTVAVIGGRGGAGASTLAA
                     ALAVTAMRGGRRAMLVDADPCGGGADLLFGGEHAAGLRWCDIAGASGRIGGEELHAAL
                     PHMGSLAVLSYDRGDPLDLRADAMSSVLSAAARVCDLTVADLPRHFDDATGVVLDVAD
                     LALLVVPAEVRAVASAARIATEVATAVDDVRVVVRGPAPGGLAALDVAHALGLPLAGY
                     LKAESNLGARLERGKAPAGQGRGPLAQFCRDLLASLTFPADRVRDPAADVSVDWTDTT
                     AGGVETAGWRPTAAP"
BASE COUNT          453 a         1067 c          974 g          399 t
ORIGIN      
        1 ctggtggccc gcgacgaccc gctcgacacc tacttggtgc accaccccga cgcgctgttc
       61 gggccgccgg tcgaggccgc ggtgttcgat cccgataacc cctacgtgct cgggccccat
      121 ctgtgcgcgg cggcgagcga gtggccgctc tcgccggacg accttgacct cttcgggccg
      181 accgcaaccg acgtggtggc cgatctggtc gaccgcaggc tgctgcgagc gcggccgaac
      241 ggctggttct ggaccaagcg cgagcgcgcg tgtgatctgg ccgacatcag gggcaccggc
      301 ggcgcgccgg tccgcatcgt cgacgaggcg accggtcagc tgctcggcac cgcgaatgct
      361 gcggccgccg actccaccgt gcacacgggg gcggtctacc tgcatcaagg cgagacgcat
      421 gtcgtgcgta ccttcgacgt cgacgaccgc gtcgcactcg ttgaaccggc ggacgtcgac
      481 tacttcacct ccgcccgcga catcaccgac gtcagaatta ctgccacgtc gcgaacgacg
      541 ctgtggggcg cagccgaagt gagcttcggt tcggtcgacg tgacaacgcg tgtcgtgtcg
      601 ttcctgcgcc gtcgcgttgt gagcggagaa gtgctcggtg aacaggctct cgacctgccc
      661 gagcggcagc tctcgacccg cgccgtctgg tggacgatcg gcgagcagga cctcgcgcga
      721 gccgggttcg tctgggctga tctggcgggc gctgcgcacg ccgccgagca tgcttcgatc
      781 ggtctgttac cgctggtcgc cacctgcgat cggtgggaca tcggcggcgt ctcgacagcg
      841 ctgcatgagg acaccggtcg gctgacggtc gtggtctatg acggctaccc gggcggggcg
      901 ggattcgccg agcgcgggta cgaggcggcg gcgatctggt tgaccgcgac ccgcgaggcg
      961 atcgcggcct gcgcatgcga gaccggctgc ccgtcgtgcg tgcaatcgcc gaagtgcggc
     1021 aacggcaacg acccactcga caaggcaggt gcgatccggc tgctcgacac gctgctgtcg
     1081 gatgcaccgc tgccggacgc gttgtcggat gcaccgctgt cggacctgcc acttccagac
     1141 aggccctccg ccatcaccct ggccctgccc gcgcacgcgc gctgacggcg tcgagcccga
     1201 gtccgcgtag cgagccaagt gccgagattc gcacgctcgt ggtgacgtcg acgacctgcc
     1261 caatcacctg acagctcgtg acgatcgcgt cgctggctag ggcgatggcg ttggccgccg
     1321 cgcacgctcc cgccgttgtt cggatcaccg gcggagtcac cggtcagatc cctgcgtccg
     1381 atcaccgagg cgcggaattc ggaacgacag gcacacaccg ccaggcgaaa ccggcggcag
     1441 cacggcatgc aagcagacgc cgtcggcgag ccggatgtcg gcctacggca ccgcgtcgtc
     1501 gaggcgacgt ccggccgccc ccgcgagcct ggcagccagc cgacggattt tgcgatcacc
     1561 gccgaggtga acgtcagcac gctcgagccc ccgaccatgg tcgacccata cctcttcggg
     1621 gccgttgacc agcacgtcgg tgacgccggt gcgcgcagcc gcgcttcgag aggaccggcg
     1681 ccgatgagct cgctccgcaa tgtctcgacc gcggcgagca cctgcgccgc acctcgcacg
     1741 ccggcctctt cccggaccag cgcggccacc cgcgccgagg ttggcggcgt gacgtcgtct
     1801 gcgagccggc tccgcacctt cgccaccaag gccgcgtcca gcgtcatggc gctgctgtcg
     1861 gacgccagcc ggccgtctca acgccgccgg cggtggtgtc ggtccaatca accgacacat
     1921 cggccgcggg gtcgcgcacc cgatcggcgg ggaacgtcaa cgatgcgagt agatcgcggc
     1981 agaactgggc gagtggtccc cggccctgac ccgcgggcgc tttaccgcgt tcgagcctcg
     2041 cgccgagatt cgactccgct ttgaggtagc ccgccaacgg cagaccgagc gcatgcgcaa
     2101 cgtcgagcgc ggccagtccg cccggtgcgg gacctctgac gacgactcga acatcgtcaa
     2161 ccgccgtggc gacctcagtg gcgatccggg cggcggaagc cactgcacga acttctgccg
     2221 ggacgacgag caacgcgaga tcggccacgt cgagaacgac gccggtagcg tcgtcgaaat
     2281 gccggggcag gtcggccacc gtcaggtcgc acacccgagc ggcggccgag agcacgctcg
     2341 acatcgcatc ggcccgcagg tcgagcggat cgccacggtc atacgacaag accgcgagtg
     2401 agcccatgtg cggcaacgcg gcgtgcagct cctcgccccc gatccggccg ctggcgccgg
     2461 caatgtcgca ccaccgcaaa cccgcggcat gctcaccacc gaagagcaga tcagccccac
     2521 cgccgcaagg atccgcgtcg acgagcattg cccgacgtcc gcctcgcatg gccgtgactg
     2581 cgagagcagc agcaagcgtg ctcgcacccg ctcccccacg cccgccgatc acggcgaccg
     2641 tcagccccaa cgcggcggat tcgtcgacag cggcggccat tcgcgcgatc agccacggct
     2701 cggcgtccgg cagcagcgcg acgtgctcgg cgccgacgcc caccgcccgt ttccagacgg
     2761 tgccgtcatc gaggtctcgc cccacgagca cgacaccgac ccggcgccgc acggatgcgc
     2821 ggccgagcgt gatcgacacg tcgtcgccga ccagcacgag ggctgccttc gtccacccca
     2881 gccgggcgcc ggc
//
LOCUS       DATCBJ010000066         2359 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_285609, whole genome shotgun sequence.
ACCESSION   DATCBJ010000066 DATCBJ010000000
VERSION     DATCBJ010000066.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2359)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2359)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2359
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_285609"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..635)
                     /locus_tag="VII50_01470"
     CDS             complement(<1..635)
                     /locus_tag="VII50_01470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5049539.1"
                     /translation="MSVELDERLLDDPDALAAADLAGMLRTIAGSGAQVREASTLVGE
                     TDVESLSDERPRAVVLSAVGSSAAACEVLRALVADDVSLPLIEASGPTLPRWVGPLDL
                     VIAVSRSGTARPTLDAVTEAGRRGCRVVTIAAPRSPLDVLGDRVRGLTLAVDKADRHT
                     RTSLWSLAIPLLAVADALGLAAVSLDEMALAADRLDATAEACRPSSESFVNP"
     gene            complement(635..844)
                     /locus_tag="VII50_01475"
     CDS             complement(635..844)
                     /locus_tag="VII50_01475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trm112 family protein"
                     /protein_id="HEY5049540.1"
                     /translation="MNLEPSLLEILACPACHSPLTIERLPDAPKDADAEELVCTNTEC
                     RRAYPVRGDIPVLLIDEARILPVAG"
     gene            complement(857..2209)
                     /locus_tag="VII50_01480"
     CDS             complement(857..2209)
                     /locus_tag="VII50_01480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009083041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HEY5049541.1"
                     /translation="MHDLSKIVKAYDIRGVVPDELDAEVARAVGAAFAIFTKAPRIVI
                     AHDMRESSGPLSQAMAEGAASQGTDVVMAGLASTDMIYFAAGSLDIPGVMLTASHNPA
                     KYNGIKLCLAGARPVGQETGLADIRALIENGIPAYSGTPGTITTHELVEDYAVFLRHL
                     VDLSGIRPLKVVVDAGNGMGGYTVPIVLKDLPLDIVPMYFELDGNFPNHEANPIDPKN
                     TADLQAKVLETHSDIGLAFDGDADRCFVIDERGQIVSPSVITALVAVRELKKSPGSAI
                     IHNLITSKAVAEIVREHGGVPVRSRVGHSFIKATMGETNAVFGGEHSGHFYFRDFWRA
                     DSGMLAALHVLAALGETPPGTKLSEFLAEYSRYVASGEINSTVRDQAAVSAKVRAAYI
                     DAPDATIDDLDGMTVTTPTWWFNLRASNTEPLLRLNVEADDQATLDRVRDEVLALIRG
                     "
BASE COUNT          414 a          789 c          772 g          384 t
ORIGIN      
        1 gggttcacga acgactcgct gctgggccgg caggcctccg cggtggcatc gagccggtcg
       61 gccgcgagtg ccatctcatc gagcgatacc gccgcaaggc cgagcgcatc ggccaccgcg
      121 agcaacggaa tcgcgagtga ccacaggctg gttcgggtgt gccggtcggc tttgtcgacc
      181 gcaagcgtga gaccacgaac acgatcaccg aggacgtcga ggggcgagcg tggtgccgcg
      241 atcgtgacga cgcggcaacc gcgccgaccg gcctcggtca ccgcgtcgag cgtcggccgc
      301 gcggtccccg atcgggacac cgcgattacc aggtcgagcg ggcccaccca gcgcggcaac
      361 gtgggaccgg acgcctcgat aagcggcagc gaaacgtcgt cggcaaccag cgctcgcagg
      421 acctcacacg cggccgcact cgagccaacg gccgagagca ccaccgcgcg cggtcgctcg
      481 tcggacagcg actcgacgtc cgtttcgccg acaagcgtcg atgcctctct cacctgggca
      541 cccgatcccg cgatggtccg gagcatgccg gccaggtcag ctgcggcgag cgcgtcagga
      601 tcgtcgagca ggcgctcgtc gagctcgacg gacatcagcc ggcaaccggc aggatccgcg
      661 cctcgtcgat cagcagaacc ggaatgtcgc cgcgtaccgg gtaggcacgg cgacactcgg
      721 tgttggtgca gaccagctcc tcggcgtcgg catctttggg tgcgtcgggc agccgttcga
      781 tcgtgagcgg cgaatggcac gccggacacg ccaggatctc cagcaatgac ggttcgagat
      841 tcatgaagtc ctccgatcag ccgcgaatga gtgcgaggac ctcgtcgcgc acccggtcga
      901 gcgtcgcctg gtcgtcggcc tcgacgttca agcgcagcaa cggctcggtg ttcgacgcac
      961 gcaggttgaa ccaccacgtc ggcgtcgtga ccgtcatgcc gtcgaggtcg tcgatcgtcg
     1021 catctggcgc atcgatgtag gccgcgcgca ccttcgcact caccgcggcc tggtcgcgca
     1081 ccgtcgagtt gatctcgccc gacgccacgt accgcgaata ctccgcaaga aactccgaca
     1141 gcttcgtccc cggcggcgtt tcccccagag cggcaagaac gtgcaaggca gccagcattc
     1201 ctgagtctgc gcgccagaaa tcgcggaaat agaaatggcc ggaatgctcg ccaccgaaca
     1261 ccgcattcgt ctcgcccatc gtggccttga tgaacgagtg cccgacgcgg ctacgcaccg
     1321 gaactccgcc atgctcgcgc acgatttctg caacggcctt tgaggtaatc aggttgtgga
     1381 tgatggctga cccaggactc ttcttcaatt cgcggacggc gaccagtgcc gttatgacgg
     1441 acggcgacac gatctggcca cgctcgtcga tgacgaaaca gcggtcggcg tcaccgtcga
     1501 acgcgagacc gatgtcggag tgcgtctcga gcaccttcgc ctgcaggtcg gccgtgttct
     1561 tcggatcgat cggatttgct tcatggttgg ggaaattccc gtccagctcg aagtacatgg
     1621 gcacgatgtc gagcggcagg tccttcaaca cgatcggcac ggtgtagccg cccatgccgt
     1681 tacctgcatc gacgacgacc ttgagtggcc ggatgccgct gaggtcgacg agatgccgca
     1741 ggaacacggc gtagtcctcg acgagctcat gcgtcgtgat cgtgccgggc gtgccgctgt
     1801 acgccggaat cccgttctcg atcagcgcgc gaatgtccgc gagcccggtc tcctgcccga
     1861 ccggtcgggc gcccgcgaga cacagcttga tgccgttgta cttggccggg ttgtggctgg
     1921 cggtgagcat cacgccaggg atgtcgaggc tgccagccgc gaaatagatc atgtcggtgc
     1981 tcgcgagccc ggccatgaca acgtcagttc cctggctggc ggcgccttcg gccatggcct
     2041 gcgacaacgg gcctgacgac tcgcgcatgt cgtgcgcgat cacgatccgc ggcgccttgg
     2101 taaagatggc gaacgccgcg ccaacagcgc gcgcaacttc cgcgtcgagc tcgtcgggta
     2161 ccacaccgcg gatgtcgtac gccttgacga tcttggagag gtcgtgcact aggccctgcc
     2221 tcggtttcgc ccgtctgccg ccagccaccc ggcgggcgca cagggaatgt gcgcgtgagc
     2281 gcagcctagc ccgccatgat ccagcgatga tgaggagatg acgtggaccg cacccaaccc
     2341 gccttcgctt gtggacggg
//
LOCUS       DATCBJ010000067         3295 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_249188, whole genome shotgun sequence.
ACCESSION   DATCBJ010000067 DATCBJ010000000
VERSION     DATCBJ010000067.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 3295)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3295)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3295
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_249188"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..881
                     /locus_tag="VII50_01485"
     CDS             <1..881
                     /locus_tag="VII50_01485"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HEY5049542.1"
                     /translation="VGARQVLASGKAIALGPRDLRCTDGIDCPLRSGVVVPLTSGGVV
                     VGTLVALAPSTRAGLLRAAGEVARFASTQLELAELDSARARAAEAELRFLRAQISPHF
                     VYNALTAIASFMRSDPDRARDLLLGFADFIRYSFRSRGQFATIADELQAVDTYLELER
                     ARFGDRLQVILRIAPEVLAVAVPCLLIQPLVENAVRHGLENKEGVGHVTISAQDGDVE
                     CILSVEDDGMGMDPEIVRDQLAGRRDHGVGLVNVDERLRQVFGADHGITVETALGAGT
                     KIIVRIPKYRAGVRAS"
     gene            878..1684
                     /locus_tag="VII50_01490"
     CDS             878..1684
                     /locus_tag="VII50_01490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009073893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HEY5049543.1"
                     /translation="MTASTSSYQPEPTVGGLRVLVVDDEAPARADLEFLLHEQPAVAT
                     VDVAADATEALRRLQADHYDGVFLDIMMPGLDGLELARVLLRFADPPVVVFVTAYDSH
                     AVAAFEARARGYLLKPVSSERLADAVALIADARRPPQSKAGDGVPPAGNDLSVVPVDS
                     GGRTWMVERADVCWIQANGDYVRLHTVQGGSHLVRLPLGLFEERWADYGFVRIHRSHL
                     VALRHVVEVRTGSAGEAVVRVAGQDLTVSRRHTAELKHRLLRVQHGGRQQ"
     gene            1681..2082
                     /locus_tag="VII50_01495"
     CDS             1681..2082
                     /locus_tag="VII50_01495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5049544.1"
                     /translation="MTADPPSEPAGAPAPPQRSIVRSSDVVTARRGGANPPAFDEHTE
                     YRDVLLHSLMRAQFGLTLGFIALAVGVLISLPLVVSLVPSLADKHVFGLPLTLAVLGV
                     AVYPVLVVIAVAYVRLAERTERHFLDLVEKL"
     gene            2108..>3295
                     /locus_tag="VII50_01500"
     CDS             2108..>3295
                     /locus_tag="VII50_01500"
                     /inference="COORDINATES: protein motif:HMM:NF012686.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation acetate symporter"
                     /protein_id="HEY5049545.1"
                     /translation="MTVAPLALAAIIIVTLITMIVGALGIRVARTPDDFMVAARAVPP
                     VRNAAAISGEYLSAASFLGVAGLILKDGLGMLWYPVGYAAGYLMLLLLVAAPLRRFGA
                     YTIPDFAEGRLGEPRLRKVATAFVLVIGWFYLLPQLKGAGLTLNVLLGTPYWVGVGVL
                     AIVVTSNIAAGGMKGVTFVQALQYWIKLVAIAVPAVVLLAVVGGVHREALTRPVPPVF
                     ATATTVHVQTSVDIKVITPVRVTAHGTIDGVQQDGPVTLIPGKYEIKDGAVLVFPAGA
                     ATPHATGLPILDGKSWVSPFVDAGHRGSHPLAATYSLIVATFLGTLGLPHILVRFYTN
                     QDGRAARRTTVIVLMLLGSFYVFPAVFAVLGRLRDPSLYLTGQTDSVVLVLPHTLIGG
                     IGGS"
BASE COUNT          486 a         1073 c         1107 g          629 t
ORIGIN      
        1 tggtgggcgc gcggcaagtg ctagcgagtg gcaaggcgat cgccctcggc ccgcgcgacc
       61 tacggtgcac cgacgggatc gactgtccct tgcgttctgg cgtcgtcgta ccgctcacca
      121 gcggcggtgt ggtcgtcggg acgctcgtcg cgctcgcgcc atcgacccgc gccggactgc
      181 tccgggccgc cggcgaggtg gctcgtttcg cgtcgacaca gctggaactg gccgagctcg
      241 acagcgcgcg ggcgcgcgcc gctgaggctg agctgcgctt tctgcgcgcg cagatctccc
      301 cacatttcgt ctacaacgcg ctcacggcga tcgcgtcgtt catgcgctcc gatcccgatc
      361 gggcacgcga tctcctgctc ggcttcgccg acttcatccg gtacagcttc cgcagtcgtg
      421 gtcagttcgc gaccatcgcc gacgaactgc aagcggtcga cacgtacctg gaactcgaac
      481 gcgcccggtt cggcgatcgt cttcaggtga tcctccggat cgcccctgaa gtgctcgcgg
      541 tcgcggtacc gtgcctgctc atccagccgc ttgtcgagaa cgcggtacgg cacggcctgg
      601 agaacaagga aggcgtcggc cacgtgacca tctccgcgca ggacggggac gtcgagtgca
      661 ttctgagcgt cgaggacgac gggatgggga tggatccgga gatcgtgcgt gaccaactcg
      721 ccgggcgccg cgaccacggc gtcggcctgg tcaacgtcga tgaacggttg cgccaggtgt
      781 tcggcgcgga ccacgggatc acagtcgaaa ccgcgctcgg agccggcacg aagatcatcg
      841 tccggatccc caagtaccgg gctggggtcc gagcttcgtg acggcatcga cgtccagcta
      901 ccaacccgag ccgaccgtcg gcggcctacg ggtgttggtc gtcgatgacg aagctccggc
      961 ccgcgcggat ctggagttcc tgctgcacga acagccagcc gtggcgaccg tcgatgtcgc
     1021 tgcggacgcg accgaggcat tacggcggct gcaggccgac cactacgacg gggtctttct
     1081 cgacatcatg atgcccggcc tcgacgggct cgaactcgcg cgagtgctgc tgcggttcgc
     1141 cgatcctccg gtggtcgtgt tcgtcaccgc ctatgactca cacgccgttg cggccttcga
     1201 ggcgcgggcc cgcggctacc tgctgaaacc ggtgtccagc gagcgcctcg cggatgcggt
     1261 ggccctgatc gccgacgcac ggcggccccc gcagagtaag gcaggcgatg gtgttccgcc
     1321 cgcgggcaac gacctctccg tagtaccagt cgattccggt ggccgtacgt ggatggtcga
     1381 acgtgccgac gtgtgctgga tccaggcgaa cggtgactac gttcggctgc acaccgttca
     1441 aggcggttcg caccttgttc ggctgccact cggactgttc gaagagcgct gggccgacta
     1501 cggcttcgtc cggatccacc gcagccatct agtcgcgttg cgccatgtcg tcgaagtgcg
     1561 caccgggtcg gcaggtgagg cagtcgtgcg cgttgccgga caggacctga cggtgagcag
     1621 acggcatacc gcggagttga aacaccgact gcttcgcgtg cagcatggtg ggcgccagca
     1681 gtgactgccg acccgccatc cgagccggcc ggggcacctg cgccgcccca gcgatcgatc
     1741 gtgcggtcgt cggacgtcgt taccgcgcga cgcggcggtg cgaacccgcc ggctttcgat
     1801 gagcacacgg agtaccgcga cgtgctgctg cactcattga tgcgtgcgca gttcggtctg
     1861 acgttgggat tcattgcact cgcggtcggt gtgctcatct cgctgccgct cgtcgtcagt
     1921 ctggttccgt cgctcgccga caaacacgtg ttcggcctgc cgctcacgct tgccgtactc
     1981 ggcgtcgcgg tgtatcccgt gctggttgtt atcgcggtcg cgtatgtgcg attggcggaa
     2041 cgaaccgaac ggcactttct cgaccttgtc gagaagctat gaagtgcctg gatttggtcg
     2101 agaagctttg acggtcgcgc cgctcgcgct cgccgccatc atcatcgtga cgctgatcac
     2161 gatgattgtt ggagcgctcg gcatccgggt tgcccgaact cccgacgact tcatggtggc
     2221 agcgcgcgct gtcccacctg ttcgcaatgc cgcggcgatc tccggcgagt atctgtcggc
     2281 ggcctcgttc ctcggcgtcg ccggactcat actcaaggac ggcttgggga tgctctggta
     2341 cccggtcggt tacgcggccg gctatctgat gctgctgctg ttggtcgcgg cgccgcttcg
     2401 acggttcggc gcctacacga tccccgactt cgcggagggc cggctgggcg agccgcgatt
     2461 gcgcaaggtc gcgacagcat tcgtgctcgt catcggctgg ttctatttgc tgccacagct
     2521 gaaaggcgcc ggcctcacgc tgaacgtctt gctcggcacg ccgtactggg tgggggtcgg
     2581 cgtgctggcg attgtggtca ccagcaacat cgcagcaggc ggtatgaaag gggtgacctt
     2641 cgttcaggcg ctgcagtact ggatcaagtt ggtggcaatc gccgtccccg ctgttgtcct
     2701 tctggcggtt gtcggcggcg tgcaccgcga agcgctgacg cggccggtgc cgccggtatt
     2761 tgcaaccgcg acaactgttc acgtgcagac gtccgtcgac atcaaggtga tcacgccggt
     2821 gcgagtcact gcccacggaa caatcgacgg cgtgcagcag gacggtccgg tgaccttgat
     2881 tccaggaaag tacgagatca aggacggcgc ggtgctggtg tttccggcgg gcgcagccac
     2941 tccccacgcg accggattgc ccatcctgga cggcaaatcg tgggtgtcgc cgttcgtcga
     3001 cgccggccat cgcggctcgc acccgttggc ggcgacgtac tcgctgatcg tggcgacctt
     3061 ccttggcacg ttggggttgc ctcacatcct ggtgcgcttc tacacgaacc aggatggtcg
     3121 ggccgcgcgc cgcacgacgg tcatcgtgtt gatgctgctg gggagcttct atgtgttccc
     3181 ggccgtcttc gccgtgctgg gacgactgcg tgatccgagc ctctacctca ccggccagac
     3241 cgactcggtg gtgttagtac tgccgcacac gctgatcggc ggcatcggcg ggtcg
//
LOCUS       DATCBJ010000068         2109 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_470698, whole genome shotgun sequence.
ACCESSION   DATCBJ010000068 DATCBJ010000000
VERSION     DATCBJ010000068.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2109)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2109)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2109
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_470698"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..537)
                     /locus_tag="VII50_01505"
     CDS             complement(<1..537)
                     /locus_tag="VII50_01505"
                     /inference="COORDINATES: protein motif:HMM:NF014774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heterodisulfide reductase-related iron-sulfur
                     binding cluster"
                     /protein_id="HEY5049546.1"
                     /translation="MKVAYWPGCVSRGFTPELHGSMAKVAPLLDLELVELDRAACCGA
                     GVIAEHNQELADTLNARTFALAQQTDGELMMNICSTCQGAQSECQERLDANTDYRNQI
                     NQNLADEGLSYSKGLTNKNLLWLLVEEIGLDTLKAKVKRPLTDLKVGPFYGCYIVRPV
                     HRLGIDAEHPRDRYMHMVI"
     gene            complement(538..1782)
                     /locus_tag="VII50_01510"
     CDS             complement(538..1782)
                     /locus_tag="VII50_01510"
                     /inference="COORDINATES: protein motif:HMM:TIGR00384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase/fumarate reductase
                     iron-sulfur subunit"
                     /protein_id="HEY5049547.1"
                     /translation="MAEYTLKIRRFDPQSGEAAYWDEHQIDLAPTASVLDAILKVRDE
                     VDGSVGIRCSCQQAICGSCGVRMNGKPGLACNTHLDEAAARTHGAGWNPEDEANGDLT
                     PPKVITVEPMGNMPVIRDLIVDMDAVHWKKIQRVTPFLINKEPVPEREYIVPHANMVD
                     VTQTMACIQCGACVSDCLSMEIDPLFVGPAALSKAYRFVGDPRDAEHFERLNDLSQDE
                     HGIYDCTHCFNCIDACPKGVDPMSQIMRLRRKAGSDHKIVDVNNGHRHEDAFVTLVKD
                     YGLLNEAELLPRSYGGNSWFGKFHPAAGKELLSSLGTVVTGVLRGKMSPKIALFGHKL
                     PKDDLASLNRIYSTVEGRPERVELNLYVSGYEDDPAETAGAETPPTVTESAEPDSAPA
                     DKSPTFPGPDGQGEPGPSESEN"
     gene            complement(1788..>2109)
                     /locus_tag="VII50_01515"
     CDS             complement(1788..>2109)
                     /locus_tag="VII50_01515"
                     /inference="COORDINATES: protein motif:HMM:NF014907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5049548.1"
                     /translation="QKAHEIIRRLKEEAPKASIDDRGTVFNQDVIGAIELGYMLDVAE
                     TIVVAAQERKESRGSQYRTDFLGRNDDEWLKHIDITANGGDVPKVGYSPVTITKWEPQ
                     ERTY"
BASE COUNT          312 a          662 c          720 g          415 t
ORIGIN      
        1 gatgaccatg tgcatgtacc ggtcgcgggg atgctcggcg tcgatcccga ggcggtgcac
       61 cggacggacg atgtagcagc cgtagaaggg gccgaccttg aggtcggtga gcgggcgctt
      121 gaccttggcc ttgagggtgt cgagcccgat ctcctccacc agcagccaca gcaagttctt
      181 gttggtcagg cccttggaat agctcaggcc ctcgtcggcg aggttctggt tgatctggtt
      241 ccggtagtcg gtgttggcgt ccagccgctc ctggcactcg gactgcgcgc cctggcaggt
      301 ggagcagatg ttcatcatca gctcgccgtc ggtctgctgg gcgagcgcga acgttcgggc
      361 gttgagggtg tcggcgagct cctggttgtg ctcggcgatg acgccggctc cgcagcacgc
      421 agcgcggtcc agctcgacca gctcgaggtc gagcagcggc gcgaccttgg ccatcgagcc
      481 gtgcaactcc ggcgtgaacc cgcggctcac gcagccgggc cagtaggcaa ccttcatcta
      541 attctcactt tcgctcggac cgggctctcc ctgcccgtcg ggtcccggga aggtcggtga
      601 cttgtcggcc ggtgcggaat cgggctcggc gctctcggtc accgtgggcg gtgtctccgc
      661 gccggctgtc tcggccgggt cgtcctcgta gccgctcaca tacaggttga gctcgacgcg
      721 ctctggacgg ccctccaccg tggagtagat ccggttcagg gaggccagat cgtccttggg
      781 cagcttgtgg ccgaacaggg cgatcttcgg gctcatcttg ccgcgcagca cgccggtgac
      841 cacggtgccc agcgaggaga ggagctcctt gccggcggcg gggtggaact tgccgaacca
      901 cgagttaccg ccatagctgc ggggcaggag ctcggcctcg ttgagcaggc cgtagtcctt
      961 gacgagggtg acgaacgcgt cctcgtgccg gtggccgttg ttgacgtcga cgatcttgtg
     1021 gtccgagccg gccttacggc gcaggcgcat gatctggctc atcggatcca cgcccttggg
     1081 gcaggcgtcg atgcagttga agcagtgcgt gcagtcgtag atgccgtgct cgtcctgaga
     1141 caagtcattg aggcgctcga agtgctcggc gtcccgcggg tcgccgacga agcgataggc
     1201 cttggacagg gcggcgggac cgacgaacag gggatcgatc tccatcgaca gacagtccga
     1261 gacgcaggcc ccacactgga tgcaggccat cgtctgggtg acgtcgacca tgttggcgtg
     1321 gggcacgatg tactcgcgct cgggcaccgg ttccttgttg atcaggaacg gggtcacgcg
     1381 ctggatcttc ttccagtgca cggcgtccat gtcgacgatc agatcgcgga tcacgggcat
     1441 gttgcccatc ggctccacgg tgatcacctt cggcggggtg aggtccccgt tcgcctcatc
     1501 ctccggattc cagcccgcac cgtgcgtccg ggctgccgcc tcatcgaggt gggtgttgca
     1561 ggccagtccg ggcttgccgt tcatgcgcac gccgcatgag ccacagatgg cctgctggca
     1621 ggagcagcga atgccgacgg atccatcgac ctcgtcgcga accttgagga tcgcgtcaag
     1681 cacggaggcg gttggcgcca ggtcgatctg gtgctcgtcc cagtacgcgg cttcgcccga
     1741 ttgcgggtca aagcggcgga tcttcagcgt gtactcagcc atcggactta atacgtcctt
     1801 tcctggggct cccacttggt gatcgtgacc ggcgagtagc cgaccttggg cacgtcgcca
     1861 ccgttggcgg tgatgtcgat gtgcttgagc cattcatcgt cgttgcgacc gaggaagtcc
     1921 gttcgatact gcgacccgcg cgactccttg cgctcctgcg ccgccacgac gatcgtctcg
     1981 gccacgtcca gcatgtaacc cagctcgatc gcgccgatca cgtcctggtt gaagacggtg
     2041 ccgcggtcgt cgatcgacgc cttgggcgcc tcctctttga gccggcggat gatctcgtgg
     2101 gccttctgg
//
LOCUS       DATCBJ010000069         5323 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_250591, whole genome shotgun sequence.
ACCESSION   DATCBJ010000069 DATCBJ010000000
VERSION     DATCBJ010000069.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 5323)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5323)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5323
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_250591"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(152..832)
                     /locus_tag="VII50_01520"
     CDS             complement(152..832)
                     /locus_tag="VII50_01520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY5049549.1"
                     /translation="MTSVLVIDDEAAILRALRINLSARDYTVTTASDGASGLAAMARE
                     RPDVVILDLGLPDMAGTDVIRGIRGWSTVPIIVLSANEQELQKVQALDAGADDYVTKP
                     FGMNELLARLRAATRRAAPGVDEPVVVTAEFTIDLGAKRVVREDGRDVRLTPTEWQLL
                     EVLARHGGRLVSGRQLLQDVWGPTYGTETNYLRVYMAQLRRKLEPDPARPRYLITEPG
                     MGYRLVTD"
     gene            complement(829..1965)
                     /locus_tag="VII50_01525"
     CDS             complement(829..1965)
                     /locus_tag="VII50_01525"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY5049550.1"
                     /translation="MTWRLADGAASGIGGRRLLGAFALEAAGLPVLTLCLAAVRLHLA
                     FADDLLLYLAAVIGVTLVGGFVPAVIAAIASSLLLNWYFTPPVHTWTIDSPQNILALV
                     LFVAVAVTVSSVVHLAAHRSALAAGLAAEAARAQGLAEGNRIRTALLAAVGHDLRTPL
                     ASVKASVSTLRQTDVTWSPQDQTELLATIEEGADRLNALISNLLDMSRIQTGAVHLFV
                     RPTSLEEVAPLVVQAVDDGALLRVEMSEHLPLVGADPGLLERALANLVANALRYSPPG
                     QPPTLVARAVQNGDARGAVQIDVIDHGPGVPPEQWARIVQPFEQLGDRRNGNGVGLGL
                     AVTKGFVEAMGGKLSADATPGGGLTMRIELPVSPAGASVGPGQQ"
     gene            2107..4023
                     /locus_tag="VII50_01530"
     CDS             2107..4023
                     /locus_tag="VII50_01530"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HEY5049551.1"
                     /translation="MTTSVGSVKRFFVGRPLRSQQLHETLLPKWLALPIFASDPISSV
                     AYATEEIVLVLAVGGGAAALTYAKNVAAAVALLLVIVTLSYRQICKAYPNGGGAFAVS
                     LDNFGENAALTAGSALLVDYVMTVAVSVVSGVVAITSAFPSLHRHAVALSVGFVVLLM
                     VANLRGVKESGRAFAVPTYSFIGLTFVMFAVAAAKAANGGLPRASTASQTFTHSHLAG
                     LALILLTLRAFASGCTALTGVEAISNGVPSFRKPKARNAAKTLTAMGTLAVSMFIGIT
                     VLALHVKAHAQPSGNPSVISQIAAVVFGKSNLLFYLYQAATAGILILAANTAFNGFPV
                     LSSILAQHSYLPRQLQKRGDKLVFSNGILLLGGFAIALIVGFNANIDKLIQLYIIGVF
                     TSFTLSQSGMVKHWKHKLAEAPRSERRSIRTSRVINFIGAAATALVLAIVLYAKVPDG
                     AWIAIVAMVLLFAVMKGIRRHYSAVSEELDIEAAENPTMPSRNHAVVLVSRLHLPALR
                     ALAYAKATRPSTIEAVTINADEAEAKTLQEQWQARGIDVPLVLLDSPYREVTRPLLEY
                     IRGRRGQSPRDVVTVFIPEYVVGHWWEQLLHNQSALRLKARLLFEPGVMVTSVPWQLH
                     STDRLRTESTTGAE"
     gene            4077..5048
                     /locus_tag="VII50_01535"
     CDS             4077..5048
                     /locus_tag="VII50_01535"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEY5049552.1"
                     /translation="MEYTTLGRTGLQVSRISLGCMSYGEPGRGNQPWSLPEHESVPFF
                     RQAIDAGINFFDTANVYSLGSSEEITGRAVAQFAPREDLVIATKVHGVMRPGPNGGGL
                     SRRSILTEIDHSLRRLGTDYVDLYQIHRWDYETPIEETLEALHDVVRAGKARYIGASS
                     MYAWQFSKALYLAEMHGWTKFVSMQNHYNLLYREEEREMIPLCIDQGIGIIPWSPLAR
                     GRLAREWDDSTNRSEKDEFGRSLYSDDDRIVVDAVGQVADQRGIPRAQVAIAWALAKP
                     GITAPIVGATKPHHLADAIAAVDVHLTPDEVELLEKPYQPHNVSGFR"
BASE COUNT          936 a         1781 c         1702 g          904 t
ORIGIN      
        1 gtcaagtgcg ccgttgagga taacgactag ggcctcgccg tcggcgacga actgcagcga
       61 ttcaccggcc tcgaaatcgg aaatctcgag cgtgatgagg tcggtgtcct tgccccgcgt
      121 gatcagagtg cgcacccagc catcctctcg ctcagtcggt tacgaggcgg tagcccatac
      181 ccggttcggt gatcaggtag cgcggccgcg ccggatcggg ctcgagcttg cggcgcagct
      241 gtgccatgta gacgcgcaag tagttggttt ctgtcccgta cgtcggtccc cacacatctt
      301 gaagcagttg gcgtccgctc acgagccggc cgccgtggcg cgcgaggacc tcaagcagct
      361 gccactccgt gggagtgagc cggacgtcgc gcccgtcctc gcgcacgacc cgcttcgcgc
      421 cgagatcgat ggtgaactcc gccgtgacca cgaccggctc gtcaactcct ggagcagcgc
      481 ggcgggtcgc tgcccgcaat cgggcaagga gttcgttcat gccgaacggc ttggtgacgt
      541 agtcgtcggc gccggcgtcg agggcctgca ccttctgcag ttcctgctcg ttcgccgaca
      601 acacgatgat cggcacggtc gaccaaccgc ggattccgcg gatgacgtcg gtgcccgcca
      661 tgtcgggcag accgagatcc aaaatcacca catccggccg ctcgcgcgcc atggcagcaa
      721 gacccgacgc gccgtccgag gcggtcgtga ccgtgtagtc gcgggccgac aagttgatgc
      781 gcagcgcacg caagatcgcc gcttcgtcgt cgatgacaag cacgctagtc attgctgccc
      841 aggcccaaca cttgcgcccg cggggctgac cggaagttcg atgcgcatcg tgagcccgcc
      901 acctggcgtg gcgtcggcgc tcagcttgcc gcccatggcc tcgacaaacc ccttggtgac
      961 agcaagtcca agaccaacgc cgttgccgtt tcgccggtcg ccgagctgct cgaagggctg
     1021 gacgatgcgc gcccactgct cggggggcac gccaggaccg tggtcaatga cgtcgatctg
     1081 gacggcgccc cgagcgtcgc cgttctgcac ggcccgcgcg acaagcgtgg gcggctgccc
     1141 gggcggggag taccgcaagg cgttggcgac gaggttggcc aacgcccgct ccagcaggcc
     1201 aggatccgcc ccgaccaggg gcagatgttc cgacatctcc acccggagca acgcaccgtc
     1261 gtccactgcc tggacgacga gtggcgcaac ctcctccagc gaggtcggcc ggacgaatag
     1321 gtgtacggca ccggtctgga tccggctcat gtcgagcagg ttgctgatca aagcgttgag
     1381 ccggtcagcg ccctcctcga tggtggcgag aagttccgtc tggtcctgcg gcgaccaggt
     1441 cacatccgtc tggcgcaatg tgctcacgct cgccttgacg gacgccagtg gtgtgcgcag
     1501 gtcgtgcccg accgccgcga gcaacgccgt acggatccgg ttgccctccg ccagcccttg
     1561 cgcgcgggcg gcttctgccg ccagaccggc ggcaagcgcc gagcggtggg ccgccaggtg
     1621 gacgacgctg ctcaccgtga ccgccaccgc gacgaacaac accagcgcga ggatgttctg
     1681 cggggagtcg attgtccagg tgtgcaccgg cggcgtgaag taccagttga gaagaaggct
     1741 gctcgcgatc gccgcgatca ccgccggcac gaagccgcct accaacgtga cgcctatgac
     1801 ggcagccaga tagagcagaa ggtcgtcggc aaaagcaaga tgaagtcgca cggccgccag
     1861 acacagggtg aggacgggaa gaccggccgc ctcgagagcg aacgcgccga ggagtcggcg
     1921 gccgccgatg cctgaagcgg cgccgtccgc aagtctccac gtcacgactc gagtgtggcc
     1981 ctgtcacgac cagatcaccc tgcagggcgg ccgcgccacg atcttgacgg ttccttgacg
     2041 gcctgcccag gtgatcttga cgttgcgctg acgccgtcgc agccccgccc tactagcatc
     2101 cagacagtga ctacctccgt cggcagcgtg aagcgattct tcgtcggtcg accacttcga
     2161 agccagcaac tccacgagac gctgctgccg aaatggctcg cactgccgat ctttgcgtcc
     2221 gacccgatct cgtcggtggc gtacgcgacc gaggaaatcg tcctcgtcct ggccgtcggc
     2281 ggcggggcag ccgccttgac ctacgccaag aacgtcgcag ccgcggtcgc actcctgctg
     2341 gtcatcgtga ccttgtccta ccgccagatc tgcaaggcgt atcccaacgg tggcggcgcc
     2401 ttcgcggtca gcctcgacaa cttcggagag aacgccgcgc ttacggccgg cagcgccttg
     2461 ctggtcgact acgtcatgac ggttgccgtg tcagtggtct ccggggtcgt cgccatcacc
     2521 agcgcgttcc cgtcgctgca tcgccatgcg gtcgcgctgt cggtcggctt cgtcgtcctg
     2581 ctgatggtgg ccaacctgcg cggcgtcaaa gaatccggcc gcgcattcgc cgttcccacc
     2641 tactcgttca tcggtttgac gttcgtcatg ttcgcggtcg ccgctgctaa ggcggcgaac
     2701 ggcggccttc cccgggcatc gaccgcctcg caaacattca cccatagtca tctggccggg
     2761 ctcgctctga tcctgctgac cctgagggca tttgcctcgg gctgtacggc cctgaccggc
     2821 gtcgaggcca tcagcaacgg agtgccgtcg tttcgcaagc cgaaggcgcg caatgccgcg
     2881 aagacgctga cggcgatggg taccctcgcg gtcagcatgt tcatcggcat cacggttctt
     2941 gctttgcacg tcaaggcgca tgcccaaccg agcggcaatc cgtcggtgat ctcgcagatc
     3001 gccgccgtcg tgttcggtaa gagcaacctg ctgttttacc tgtaccaggc cgcgactgcg
     3061 ggcatcttga tcctcgccgc caacaccgcc ttcaacggat tcccggtgct gtcgtcaatt
     3121 ctcgcccaac acagctatct gccgcggcag cttcaaaagc gcggcgacaa gctcgtcttc
     3181 agcaacggga tcctgctgct cggcggtttc gcgatcgcac tgatagtcgg cttcaacgcc
     3241 aacatcgaca agctcatcca gctgtacatc atcggcgtgt tcacgagctt cacactgagc
     3301 cagtccggca tggtcaaaca ctggaagcac aagctcgcgg aggcgccgcg tagcgaacgc
     3361 agaagcatcc gtacatcgcg agtcatcaac ttcattggtg ccgcggccac tgctcttgtg
     3421 ctcgcaatag tgctctatgc aaaggtgccg gacggcgcgt ggatcgcgat cgttgcgatg
     3481 gtgctgctct tcgcggtgat gaaaggcatc cggcgccact acagcgccgt cagcgaggag
     3541 ctcgacatcg aggccgccga gaacccgacg atgccgtctc gcaatcacgc ggtcgtgttg
     3601 gtctctcgcc tccacctgcc ggccctgcgc gcgctcgcgt atgccaaggc cactcgcccg
     3661 agcacgatcg aggcggtcac catcaacgcc gacgaagccg aggcaaaaac cctgcaggag
     3721 caatggcagg cgcgcggcat cgacgtccct cttgtcttac tggattcgcc ctatcgcgag
     3781 gtgacccgtc cgttactcga atacattcgc ggacgccgag ggcagagccc gcgcgatgtc
     3841 gtgaccgtct tcattcccga atacgtcgtc ggccactggt gggaacagct gctgcacaac
     3901 cagagcgccc tgcgtctgaa ggcgcgcctg ctgttcgagc ccggcgtcat ggtgaccagc
     3961 gtgccctggc agctgcactc gaccgatcgg ctgcgcacgg agtcgacgac cggggcggag
     4021 tagccgcccg agcagcgagt taggctcgat gtcttacgtg cgcgaggagg cagcggatgg
     4081 agtacacgac actaggccgg accgggttgc aggtatcgcg catctcgttg ggctgcatga
     4141 gctacggcga accgggtcgc gggaatcagc cgtggtcgct tcccgaacac gagagcgtcc
     4201 cgttcttccg gcaggccatc gacgccggca tcaacttctt cgataccgcg aacgtgtact
     4261 cgctgggcag cagcgaggag atcaccggtc gcgcggtggc gcagttcgcg ccacgcgagg
     4321 acctcgtcat agcgacgaaa gtgcacggcg tgatgcgccc ggggccaaac ggcgggggcc
     4381 tgtcgcgcag gtcgatcctg accgagattg accacagcct gcgcaggctc ggcaccgatt
     4441 acgtcgacct gtaccagatc caccgctggg actacgagac gccgatcgag gagacgctcg
     4501 aggcgctgca cgacgtcgtc cgggctggga aagcccgcta tatcggcgcg tcgtcgatgt
     4561 acgcctggca gttcagcaag gcgctctacc tcgccgaaat gcatggctgg accaagttcg
     4621 tgagtatgca aaaccactac aacctcctgt atcgcgagga ggagcgcgaa atgatcccgc
     4681 tctgcatcga tcagggaatc gggatcatcc cgtggagccc gctggcacgc gggcgcctcg
     4741 cgcgggaatg ggacgactcg acgaaccgct ccgagaaaga cgagttcgga cgcagcctct
     4801 acagcgacga cgatcggatc gttgtcgatg cggtcggcca ggtcgccgac cagcgcggca
     4861 tcccgcgcgc gcaagtggcc atcgcatggg cgctggcgaa gccgggcatc acggctccca
     4921 tcgtcggggc gacgaagccg catcacctcg ccgacgcaat tgccgccgtc gatgtccatc
     4981 tcacgccgga cgaggtcgag ctgctggaga agccctacca gccgcacaac gtctccggtt
     5041 tccgctgagg tgccggcagg tatcgacgtc cctgataggc gtggtcggat cggcctcgat
     5101 cgggtcggcc gagacctcac tcgcaatcca cgggtcgtgg ttcgcggggt ggagttaacg
     5161 tccgacgggt ggcacgtgct ccggcgaacg accttcgact accagaactc caagggcgac
     5221 tggtcaaccc agcatcggga gacttacgac cggggcaatg gcgcgaccat cttgctgtac
     5281 gacgtcggcc gccgcatggt gctgttgtcg cggcagttcc gat
//
LOCUS       DATCBJ010000070         2075 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_513393, whole genome shotgun sequence.
ACCESSION   DATCBJ010000070 DATCBJ010000000
VERSION     DATCBJ010000070.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2075)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2075)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2075
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_513393"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..966)
                     /locus_tag="VII50_01540"
     CDS             complement(<1..966)
                     /locus_tag="VII50_01540"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HEY5049553.1"
                     /translation="MNVPMLGAIVAISALVAWILFHLAHGGVVAAEQRSRGTTAQGEP
                     PAADVELLHALRALYKRMYFTAYLCGAALAGGGVAAVQWSSLGPGGRVVTGGVLLVVL
                     ALPMMFAPRPVATGYARARGISRAELRSRRTTVFRVIWMLIAAWPVPVAFAVEPMVVD
                     RVFFAAVAYLLALPLAVGVLAPFMVRLLAPHPITPDIAARLTAVASRAGIRLLTTRVI
                     ASRTRKVANALQVGWLPGLQWVVMTDYLLDSLTDADVDAVFAHELGHARGHDTWKRSA
                     LAGAMLFCLSCTIISGLAQAPNLLLAIGGLATAAVLGRVQRQIAVR"
     gene            complement(1019..>2075)
                     /gene="dop"
                     /locus_tag="VII50_01545"
     CDS             complement(1019..>2075)
                     /gene="dop"
                     /locus_tag="VII50_01545"
                     /EC_number="3.5.1.119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010498 - proteasomal protein catabolic
                     process [Evidence IEA];
                     GO_process: GO:0019941 - modification-dependent protein
                     catabolic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="depupylase/deamidase Dop"
                     /protein_id="HEY5049554.1"
                     /translation="GCHENYLTKRSTPFTEIVRHLTPFFVTRQVFCGAGRVGIGADGR
                     GHGFQISQRADFFEVEVGLETTLKRPIINTRDEPHSDPEKYRRLHVIIGDANMSEIST
                     YLKMGSTALVLAMIEDGFLTADFSIEGPVAALRAVSHDSTLSHRVTLRDGRKMTGLQV
                     QMEYLEQARKYVEDRFGADADDVTRDVLDRWESVLSRLSEDPMQLSTELDWVAKLSLL
                     EGYRDRENLNWEAHKLQLVDLQYHDVRADRGLYNRLVARGSMQTLQNEDAVVRATTQP
                     PDDTRAYFRGRCLAKFGTEVAAASWDSVIFDLPGRDSLQRIPTLEPLRGTKAHVGDLI
                     DRCATASELVAALTDSH"
BASE COUNT          385 a          668 c          689 g          333 t
ORIGIN      
        1 ccgcaccgcg atctggcgct gcaccctgcc gagaacagct gcggtggcaa gcccgccgat
       61 ggcgaggagg aggttcggtg cctgggccag accgctgatg attgtgcaag acaggcaaaa
      121 gagcatggcg cccgcgagtg cactgcgttt ccaggtgtcg tggcctcgcg catggcctaa
      181 ctcgtgggca aagacggcat cgacgtcggc gtcggtcaat gagtcgagca ggtagtcggt
      241 catgaccacc cactgcaagc cgggcagcca acccacctga agcgcgtttg ccaccttgcg
      301 ggtacgcgac gcgatgaccc gcgtggttag caaccggatt cccgctctcg acgcaactgc
      361 ggtcagcctg gctgcgatgt caggtgtgat cggatgaggt gcaagcagcc ggaccatgaa
      421 cggcgcaagt acgcccacgg caagcggtag cgcgagcaga tacgcgactg ctgcgaagaa
      481 gactcggtcg accaccatcg gctcaacggc gaaggcaaca ggcaccggcc acgccgcgat
      541 cagcatccag atgacccgga aaacggtggt gcgccgcgaa cgcagctctg ctcgcgagat
      601 tccgcgagcg cgcgcgtacc cggtggcaac cggccgtggg gcgaacatca tcggcaaagc
      661 cagcacgaca agcagcacgc cgccagtcac cacacggccg cccggcccga gactcgacca
      721 ttgcacggcg gccacgccgc cgcctgcgag cgcggcgccg cagaggtagg ccgtgaagta
      781 catccgcttg taaagcgcgc ggagcgcgtg gagcagctcg acatccgcag caggaggctc
      841 gccctgtgct gttgtaccgc gcgaacgttg ctcagcagcg acgaccccgc catgcgccag
      901 gtggaagaga atccacgcaa ccagcgccga gatggcgacg atcgcgccca acatcggcac
      961 gttcatgaac tcccccggtg aactgccttg gtgtgaatcg gctgggcgtg aaattggctc
     1021 agtgtgaatc ggtgagcgca gccacgagct cgctggcagt cgcgcaccga tcgatcaggt
     1081 cgccaacatg cgccttcgtg cctcttaagg gttcgagcgt ggggattcgc tgcaatgaat
     1141 cgcggccggg tagatcgaag atcactgaat cccacgacgc cgcggcaacc tcggtgccga
     1201 acttggcaag gcaacggcca cggaaatacg cacgagtgtc gtccggcggt tgcgtcgttg
     1261 cccgcaccac ggcgtcctcg ttctgcaggg tttgcatgct gccccgcgcc accagccggt
     1321 tgtagaggcc gcgatcggcg cgcacatcgt ggtactgcag gtcgacgagc tgcagcttgt
     1381 gtgcctccca gttcaggttc tcgcgatcgc ggtaaccctc gagcagggac agcttggcca
     1441 cccagtcgag ctcagtcgac aactgcatcg ggtcttcgga caaccgggag agcaccgact
     1501 cccagcggtc gaggacatcc cgcgtgacgt cgtcggcgtc ggcgccgaac ctgtcctcga
     1561 cgtacttgcg ggcctgctca aggtattcca tctgcacctg gaggccggtc atcttgcggc
     1621 cgtcgcgcaa ggtgacgcgg tggcttagcg tcgagtcatg cgaaaccgcg cgcagggcgg
     1681 cgaccgggcc ctcgatggag aagtcggccg tcaggaaacc gtcctcgatc atcgcgagga
     1741 ccagtgcggt tgatcccatc ttgagatagg tggagatctc gctcatgttc gcgtcgccga
     1801 tgatcacgtg gagccggcgg tacttctccg ggtcggagtg cggctcgtcg cgggtgttga
     1861 tgatcggccg cttcagcgtc gtctcgagcc cgacctcgac ttcgaagaag tcggcgcgct
     1921 ggctgatctg gaacccgtgg ccgcggccgt cggcgccgat tccgacccgg ccggcgccgc
     1981 agaaaacctg ccgagtcacg aagaacggcg tcaggtgtcg cacgatctcg gtgaacggcg
     2041 tgctccgctt ggtcaggtag ttctcgtggc agccg
//
LOCUS       DATCBJ010000071         2390 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_249106, whole genome shotgun sequence.
ACCESSION   DATCBJ010000071 DATCBJ010000000
VERSION     DATCBJ010000071.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2390)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2390)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2390
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_249106"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..860)
                     /locus_tag="VII50_01550"
     CDS             complement(<1..860)
                     /locus_tag="VII50_01550"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase"
                     /protein_id="HEY5049555.1"
                     /translation="MILVCGETLIDLVHVDGEIWRALPGGSPANTAVALARLSTSTAM
                     LARISSDAFGARLRERLVGDGVDVRYVVTAAEQSSLAVVDLADNGSARYSFYLRDTAD
                     WQWVTSDLPVAFDPSVTALHAGSMALLLPPGGVVLEAMLERERSQRVVSIDPNVRASI
                     CPDRRVYRETVERWLRLAHIVKASVDDVNWLYPDRSYADVLEDWSTRGPSLVVFTRSA
                     NGALARLPSGLEVGVGGIDVEVVDTIGAGDTFTAGLLHALDEVGLLHVSTLAQLTAEQ
                     VEEALRFAVLV"
     gene            complement(884..2044)
                     /locus_tag="VII50_01555"
     CDS             complement(884..2044)
                     /locus_tag="VII50_01555"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEY5049556.1"
                     /translation="MPGNPLVPRLQGLGTTIFAEMSALALETGSINLGQGFPDVDGPH
                     IIKQAAVDAIWAGRNQYPPGIGVPELRFAIADHQRRFYGLSLDPDIEVLVTAGATEAV
                     AAAMLAMVDAGDEVIALEPYYDSYVASIVMAGGVRVPVTLRAPDFRLDVERLRTAVTS
                     RARLILLNSPHNPTGTVLTRDELQAVASVAIEHDLYVVTDEVYEHLVYDGEHVPLATL
                     PGMWERTLTISSAGKTFALTGWKVGWVSGPSALVAAVRTTKQFLTYVNGAPFQPAVAV
                     GLGLDDSFYATVTSDLRRKRDLLCAGLASVGFSVFKPAGTYFVTTDVRPLGYDDGVAF
                     CRDLPKRAGVVAIPHSVFYDNASVGRPLVRFAFCKRDEVLVEAISRLGQLAS"
     gene            2078..>2390
                     /locus_tag="VII50_01560"
     CDS             2078..>2390
                     /locus_tag="VII50_01560"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEY5049557.1"
                     /translation="MPIALITGSTSGIGAGFAEIYAKLDHNLVLVARDRGRLDAQAAR
                     LHEQWQVDVEVLAADLGTDAGCSAVEARVADEAQPIDVLVNNAGFGMGLDFLHSALAD
                     EE"
BASE COUNT          471 a          802 c          754 g          363 t
ORIGIN      
        1 accaggacgg cgaaccgcag cgcctcctcg acctgctctg cggtgagttg cgcaagcgtc
       61 gaaacgtgca gcagccctac ctcgtcgagt gcgtgcagaa ggccggcagt aaacgtgtcg
      121 ccggccccga tcgtatcgac gacctcgacg tcgatgccgc cgacgcccac ctcgagtccc
      181 gacggcagtc gagcgagcgc gccgttcgcg cttcgagtga acacgaccag cgaaggtccc
      241 cgggtggacc agtcctcgag cacgtcggcg taggagcgat ccggatacag ccagttcacg
      301 tcgtcgacac tggctttgac gatgtgtgcg agacgaagcc atcgctcgac cgtctccctg
      361 tacacccgcc ggtcgggaca gatcgacgca cgcacattcg ggtcgatcga gaccacacgc
      421 tgcgaccgct cgcgctccag catcgcctcg aggacgacgc cccccggcgg cagcagcagc
      481 gccatcgaac ccgcatgcag agcggtgaca gacggatcga aggccaccgg taaatcgctg
      541 gtgacccact gccagtcagc tgtgtcccgg agatagaagc tgtagcgcgc gctgccgttg
      601 tcggcgaggt cgacgacggc cagcgaactc tgctccgcgg ccgtgacgac gtagcgaacg
      661 tcgacaccgt caccgaccag ccgctcccgc aatcgcgcgc cgaaagcgtc agaggaaatt
      721 cgcgcgagca tcgctgtcga cgtgctgagc cgagcgagtg cgaccgcggt gttcgcaggc
      781 gatcccccgg gcagtgctcg ccatatctcg ccgtcgacgt ggacgagatc gatgagggtc
      841 tccccgcaca ccaaaatcac cggcgacctc cgagatagga cagctaggaa gcgagttggc
      901 cgagtcgaga gattgcctcg accagtacct cgtcgcgctt gcagaaagca aaccgcacta
      961 acgggcgtcc aactgaagcg ttgtcgtaga agaccgaatg cggaatcgcg accacaccgg
     1021 ctcgcttggg caggtcgcga cagaatgcca caccatcgtc gtatccgagc gggcgaacgt
     1081 cggtcgtgac gaagtacgta ccggccggct tgaacaccga gaaacccacg gaagcaagcc
     1141 cggcacagag cagatcgcgc ttgcgccgca gatcactggt caccgtcgcg tagaacgagt
     1201 cgtcgagccc gagacccacc gccaccgcag gctgaaaagg tgcgccgttg acgtacgtca
     1261 agaactgctt ggtggtgcgc acggccgcca ccaatgccga gggtcccgac acccagccga
     1321 ccttccagcc ggtgagcgcg aatgtcttgc cggccgaaga aatcgtcagc gtccgttccc
     1381 acatcccagg caacgtcgca agaggcacat gctcgccgtc gtaaaccaag tgctcgtaca
     1441 cctcgtcggt cacgacgtac aagtcatgtt cgatcgccac cgaggcgacc gcttgcagtt
     1501 cgtcacgagt cagtacagtt ccggtcggat tgtgcggtga gttcagcaga atcagccgag
     1561 cgcgggaagt gaccgcggtg cgtagccgct cgacatccag ccggaagtcg ggtgcgcgca
     1621 acgtgaccgg tacccgcacc ccgcccgcca tgacgatcga cgcgacgtag gagtcgtagt
     1681 acggctccaa cgcgatgact tcgtcgcccg cgtcgaccat cgcgagcatt gccgccgcga
     1741 cggcttccgt tgcgcccgct gtcaccaaga cctcgatgtc cgggtcaagc gaaaggccgt
     1801 agaaccgccg ctgatggtcc gcgatggcga aacgcaattc ggggacgccg atccccggcg
     1861 ggtattgatt ccgaccagcc cagatcgcgt cgaccgccgc ctgcttgatg atgtgtgggc
     1921 cgtcgacgtc gggaaacccc tgcccgaggt tgatagaccc ggtctccagc gcgagcgccg
     1981 acatctcggc aaagatcgtg gtcccgagcc cttggaggcg cggcaccagc gggttacccg
     2041 gcacgcgggt cagcctagtg ctggcaagct ggcgtctatg ccgattgcac taattaccgg
     2101 atcgacctca gggatcggtg ccggattcgc cgagatctac gccaagctgg accacaatct
     2161 agtgctggtg gcgcgcgatc gtggccgcct cgacgcccag gccgcaaggc ttcacgagca
     2221 atggcaggtg gacgtcgagg tgctggccgc cgacctcggc accgacgccg ggtgctcggc
     2281 ggtggaagcc cgcgttgccg atgaagccca gcccatcgac gtcctggtca acaatgccgg
     2341 cttcgggatg gggctcgact tcctgcactc cgcgctggcc gacgaagagc
//
LOCUS       DATCBJ010000072         4279 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_433789, whole genome shotgun sequence.
ACCESSION   DATCBJ010000072 DATCBJ010000000
VERSION     DATCBJ010000072.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 4279)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4279)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4279
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_433789"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1188
                     /locus_tag="VII50_01565"
     CDS             <1..1188
                     /locus_tag="VII50_01565"
                     /inference="COORDINATES: protein motif:HMM:NF013073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HEY5049558.1"
                     /translation="TWPAPRKKEIAHVASPQIVLAPRTTRRIARRGPRRAALLTGLTA
                     TLALTLLPTGAAQADPATTVDTAAGQAQVQALADKAEIVTEKYDASQDALAAAQTQLT
                     TDRAAIAAAQRTVDSTQGLVDAIARDAYESGALGGAASVLLSQDPQAALRGADYLERL
                     AENRDPELAQATTARNALVQAQASTNQELARVQALQVSIQAEQKTINALIGKQQAALV
                     ATQAQAAPQVAATTQASRGQARIALPKAAVVVAARAAPRAAAPAAAAPAAAAPAAAVP
                     AVASSKAAAVLRYAYAQLGKPYRYGAAGAQTFDCSGLTMRAWAAAGVAISHNAAAQYD
                     STKHVARSALQPGDLVYFGRPIHHVGIYIGGGNFIEAPYTGADIRISNLGARHDYAGA
                     SRP"
     gene            1237..2361
                     /locus_tag="VII50_01570"
     CDS             1237..2361
                     /locus_tag="VII50_01570"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEY5049559.1"
                     /translation="MTRTLVVTNDFPPRAGGIQAFVHELVARLEDVVVYAPAWEGAAA
                     FDAAQGFEVVRHPSSLMLPTRGVSRRAAALLKAHKCDAVWFGAAAPLGLLAAALRRAG
                     ADRIVATTHGHEAGWAQLPVARQTLRRIGDRVDVMTYLGKYTRSRLERALSASAQGHL
                     ARLAPGVDTSFFKPGAGGDVVRRDLGLGDRQVIVCVSRLVPRKGQDVLLRALPAVLRA
                     LPDAALLIVGDGPDRKRLEAITDREGVRAAVRFSGPVAWADLPAYYDAGQVFAMPCRT
                     RRRGLDVEGLGIVYLEASATGLPVVAGRSGGAPDAVILGETGQLVDGRDVAAVAASIV
                     ALLRDPGAAAAIGAAGRAWVERDWTWDASAGRLAQLLATS"
     gene            complement(2364..3743)
                     /locus_tag="VII50_01575"
     CDS             complement(2364..3743)
                     /locus_tag="VII50_01575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5049560.1"
                     /translation="MPPPLVPPSGLVQVAGGVEGGRAAVRPIWFDAFLVGVIALAVFA
                     ATAQYHDPLHNDAHATAVAAWQLGHHGNATLTAFRGQFPWLYAVDGRDVTNRLPGTIF
                     WAAPFYALLGTSHFPPIYPSAIAAVVASAVAIGLAFALCCRLVPRSQAAAAALALAFA
                     TGTWTVSAKELWTHGPAQAAVLMTVLCCSHRRWLAAGIPAGFAILIRPHLGVIAVVLG
                     VAAAIRERSARPLLISVGSAIGLWILLLYNHAVWGRWTVFGGYADKSQASVHALAEFG
                     VGSVGTFVSPERGVLVMTPALLLLLPGCRAAWRVAPWWVRSATVSAIAYVAVQIWVSR
                     FSGGDGFYSYRTPLEGLTLSVPLLTLAWREWTSTTRVRRTAFAGLAAASVAFHAFGAI
                     VNWVPGGPNHSPWKTFMPFDLARHIGGVQTAGWLCAAVAAVATSATLTWRAGVRVPAA
                     PEPQGAMTA"
     gene            complement(3718..3933)
                     /locus_tag="VII50_01580"
     CDS             complement(3718..3933)
                     /locus_tag="VII50_01580"
                     /inference="COORDINATES: protein motif:HMM:NF016828.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp family type IVb pilin"
                     /protein_id="HEY5049561.1"
                     /translation="MIAYMVAVRRHVKDDKGASAVEYGLLIAAIAAVIVVIVFALGSV
                     VKSKFQKTCNGINSGNTAAGATTTCTP"
     gene            complement(4137..4277)
                     /locus_tag="VII50_01585"
     CDS             complement(4137..4277)
                     /locus_tag="VII50_01585"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HEY5049562.1"
                     /translation="MARQLYISESTTKTHISKLYEKLGAANRAQALMSAMRLRLIKSP
                     EQ"
BASE COUNT          697 a         1440 c         1483 g          659 t
ORIGIN      
        1 acttggccgg cgccgaggaa gaaggagatc gcccacgtgg cgtcccctca gatcgttttg
       61 gcgccgcgca ccaccaggcg cattgccagg cgtggcccgc gccgggccgc gctgcttaca
      121 gggcttaccg cgacgttggc cctgactttg ttgcctacgg gcgccgcgca ggcggatccg
      181 gcaacgaccg tcgataccgc cgctggccag gcgcaagtcc aggctcttgc cgataaggcc
      241 gagatcgtga ccgagaagta cgacgcctcg caggacgcct tggcggccgc gcagacccag
      301 ctcaccactg atcgggccgc tatcgccgcc gcgcagagga ccgtcgactc cacccagggc
      361 ctcgtcgacg ccattgctcg cgacgcctac gaatcggggg cactcggcgg cgctgccagc
      421 gtgctgctct cccaggaccc ccaggcggcc ttgcgtgggg ccgattatct ggagcggctc
      481 gcggagaacc gcgaccctga gcttgcccag gccaccaccg cacgcaacgc gctcgtccaa
      541 gcgcaggcgt cgaccaatca ggagctcgcc cgcgtgcagg ctctgcaggt gtctatccag
      601 gccgagcaga agaccatcaa cgccctgatc ggcaagcaac aggccgcgct tgttgcgacg
      661 caggcgcagg ctgcgccgca ggtcgccgca acgacgcagg cgagccgcgg ccaggcgagg
      721 atcgccttgc ccaaggctgc cgtggttgtg gccgcgaggg cggctccgcg tgccgccgct
      781 cccgcagccg ccgctcccgc agccgccgct cccgcggccg ccgtcccggc tgtggcgagt
      841 agcaaagcgg ccgcggtgct gcgttatgcc tatgcccagc tcggcaagcc ctaccgctac
      901 ggtgcggcag gtgcgcagac gttcgactgt tcgggcctca ccatgcgggc ctgggcagcc
      961 gccggcgttg cgatcagcca caacgcggcc gcgcagtatg actcgaccaa gcacgtggcg
     1021 cgtagcgcgc ttcaacccgg cgacctcgtg tacttcggcc gcccgatcca ccacgtcggt
     1081 atctacatcg gtggtggcaa tttcatcgag gcgccctaca ccggcgccga catccggatc
     1141 tccaacctcg gtgcccgcca cgattacgcg ggtgcttccc ggccgtaatc gcgttggggc
     1201 acagcggttg atgatcggcc gccctaccat gacggcgtga cgcgcacgct cgtcgtgacg
     1261 aacgacttcc cgccgcgcgc tggcgggatt caggccttcg tccacgagct cgtggcgcga
     1321 ctcgaggacg tcgtggtgta cgcgccggcg tgggagggcg cagctgcctt cgacgccgca
     1381 caaggattcg aggtcgtgcg gcacccgtcg tccctcatgc tcccgacgcg cggcgtctcg
     1441 aggcgggccg cagcgttgtt gaaggcgcac aagtgcgacg ccgtgtggtt cggggccgct
     1501 gcgccgttgg ggttgctggc ggcggcgctt cgccgagcag gcgccgaccg gatcgtggca
     1561 acgacgcacg gccatgaagc cggctgggcg caacttccgg tggcccgaca gaccttgcgc
     1621 cgaatcggcg accgggtaga cgtcatgacc tacctgggca aatacacccg cagccggctc
     1681 gagcgggccc tgtcggcgtc cgcgcaagga cacctcgcac ggttggcacc gggtgtcgac
     1741 acatcgttct tcaagcctgg cgccggcggc gacgttgtgc gccgcgacct cggactgggc
     1801 gaccgccagg tcatcgtctg cgtgtcgcgg ttggtgcctc gcaagggcca ggacgtgttg
     1861 ctgcgcgcgt tgcccgcggt gctgcgggca ctacccgatg cggcgctgtt gatcgtcggt
     1921 gacggaccgg accgcaagcg gctcgaggcg atcaccgatc gcgaaggggt cagggccgcc
     1981 gtccgtttca gcggacccgt cgcgtgggcc gacctgcccg cgtactacga cgcaggccag
     2041 gtgttcgcga tgccgtgccg gaccagacgc agagggctgg acgtcgaggg gctcggcatc
     2101 gtgtatctgg aggcctccgc caccgggctt ccagtggtgg ccggccggtc gggtggcgcg
     2161 ccagacgcgg tgattctcgg ggagaccggc cagcttgttg acggccgtga tgtcgccgcg
     2221 gtggcggcct cgattgtcgc gctgttgcgc gatccggggg cggcggccgc gatcggcgca
     2281 gcggggcgcg cgtgggtcga gcgggattgg acctgggacg ctagcgccgg tcgacttgcg
     2341 cagctgctgg cgaccagttg aactcaggca gtcattgcac cctgcggttc gggtgctgcc
     2401 ggcacccgga cgccggctcg ccaggttaac gtcgcgctag tcgcgacggc ggcgactgcg
     2461 gcgcacagcc agccggcagt ttgaacaccg ccgatgtggc gcgcgaggtc gaagggcatg
     2521 aacgtcttcc atggtgagtg gtttggtccg ccgggcaccc agttcacgat cgctccgaac
     2581 gcgtgaaacg ccacgctcgc cgcggcaagc ccggcgaagg ccgtccggcg cacgcgggtc
     2641 gtcgaggtcc attcacgcca cgcgagtgtg agcagcggaa cacttagcgt gagtccttct
     2701 agcggggttc ggtaggagta gaagccatct ccgcctgaga atctgctcac ccagatctga
     2761 acggcgacgt aggcaatggc cgacactgtc gccgaccgca cccaccacgg cgcgacccgc
     2821 cacgcggcgc ggcagccggg caacagcaag agcagcgctg gcgtcatcac aagcaccccg
     2881 cgctccggtg acacaaatgt gccgacactc ccaacgccga actcggcgag cgcgtgtacg
     2941 gaggcctggc ttttgtcggc gtagccgcca aatacggtcc acctgcccca cacagcgtgg
     3001 ttgtacagca ggaggatcca caagccgatt gccgagccga cactgatgag tagcggtcga
     3061 gcgcttcgct cacgaatcgc cgcggcgacg ccgagcacaa cggcgatcac gccgaggtgt
     3121 gggcggatca aaatcgcgaa cccggcaggg attcccgctg ccaaccaccg tcgatgcgag
     3181 cagcacagca cggtcatgag caccgcagcc tgcgcgggtc cgtgggtcca caattccttc
     3241 gcagaaacag tccatgtacc ggtcgcaaac gcaagcgcaa gtgcggcagc cgccgcttgc
     3301 gaacgaggca cgaggcggca acacaacgcg aacgcgagtc cgatggcgac tgccgatgca
     3361 accacggccg cgatggcgct ggggtagatg ggcgggaagt ggctagtccc gagcaacgca
     3421 tagaacggtg ccgcccaaaa gatcgtgcca ggcagccggt ttgtgacgtc acggccgtcg
     3481 actgcgtaga gccacgggaa ctggccacgg aaggccgtga gtgtcgcatt gccgtggtgg
     3541 cccaactgcc atgcagcgac ggcggtggca tgagcgtcgt tgtggagcgg gtcgtggtac
     3601 tgcgccgtcg ccgcgaagac cgcgagcgcg atgacgccga cgagaaatgc gtcgaaccag
     3661 atggggcgga cggctgcgcg gccgccctcg acgccgcccg ctacctgcac aagcccgcta
     3721 gggggtacaa gtggtggtgg caccggcagc cgtgttaccg ctgttgatgc cgttgcacgt
     3781 cttctggaac ttggacttga ccacgctacc cagggcgaag acgatgacca cgatgacggc
     3841 cgcgatggcc gcgataagca ggccgtattc gacggcggac gcgcctttgt cgtccttcac
     3901 gtggcggcgc actgccacca tgtacgcgat catggacgaa cccttcgagg cggaggtgct
     3961 gacccgggct tgtcggggtc aactgccagg cgtaacccac ccagcgtctt gagcctgcgc
     4021 cctgcaactg ccacgcggta tcggacgcct ggtcgaaaac cgcgtaccga tgggggatgg
     4081 cagactgcag cattccggac tattgtgcgc ggctcgcgat tcggggggcg tgcgccctac
     4141 tgttcaggcg acttgatgag ccgcagtctc atggccgaca tcagtgcttg ggcgcggttc
     4201 gccgccccca gcttctcgta aagcttggag atgtgtgttt tcgtcgtcga ctcgctgatg
     4261 tagagctggc gggcgatcg
//
LOCUS       DATCBJ010000073         2916 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_359620, whole genome shotgun sequence.
ACCESSION   DATCBJ010000073 DATCBJ010000000
VERSION     DATCBJ010000073.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2916)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2916)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2916
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_359620"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            295..525
                     /locus_tag="VII50_01590"
     CDS             295..525
                     /locus_tag="VII50_01590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5049563.1"
                     /translation="MTPDLDVDALLCCWQKASAALIEAECRDPYSAEVDDIADEVIAA
                     RLALTQAGVRDIAALAESRHHPAQPTGVAIGF"
     gene            954..>2916
                     /locus_tag="VII50_01595"
     CDS             954..>2916
                     /locus_tag="VII50_01595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5049564.1"
                     /translation="MRKHAAGPRLSWAKVGSSGIVASLLVWGVASTLGSHGTHATRAT
                     ALASAFGSSASGYVSPSSDSEPVELAAAMPVVPGLAQAAIRQPASVGYATIAPVGGAA
                     ARAVGVAAQPAALEVTTIPRLASVAYQRAATTEAHDASSCGVSWEILAGIGLVESDHG
                     RSGGSASPSWSGVASPAIFGPVLNGQDGFPVIKDTDHGVLDGDPLFDRAVGPMQFLPA
                     TWREYDTATSGSAAPNPENISDAAFAAARYLCASGADLRTSTGLVDAIYGYNHSFAYV
                     TNVVTAAQRYAEGTLQGAAGALAELPALLSGQPTASFVTDPVVLASGTPGTSSGSPAS
                     GTSAGTSPSTTRSAIVGAASPDPDPTVDPTSDPIQDPTPAPTDSSPSDSSSTSDSPAP
                     DTTDSPAPDSSTSDPSATDSASTSDSDSPPADTSAPGTSAPDTSSPTPSLPPTPTPTP
                     TITPTPTLTPTQPTTTTPTPPPATPGGSARPGPTNTGVPAGTQLTVYNGDLTITTPGA
                     TYADLDIHGFIRVEAPDVTIKDSIIRGGPATSNTAIIYDLSDAATNLVVEDSEIMPAD
                     PSVFIDGIDGWNYTALRLNIHGTVDGAKMFGTNATIEHSWIHDLVTYAHDPSQNGGQT
                     HNDDVQILSGSNLQILGNDLEGGSNSAIQL"
BASE COUNT          529 a         1001 c          892 g          494 t
ORIGIN      
        1 gtgctcgacc ttgcgctcgc tcattcctaa acgtccgcag gtgctggtct cactccctgc
       61 tgtgcgaaag cttcctgcgt acgaggacac gctcatgcgg gcggtcgagc tctgccgggc
      121 acgcaacacc cctaccgagg ctcctgtcac gcctttgctg cgctgagatt tcgcctcgtc
      181 ttcgccccaa atttcgtcgc tggctacgcg tccaaccgat gatcaggttg tgcgctgcgc
      241 ccgacgtgcg atgtcagcgt gcgggggaac gtagccgaga tgggaaggac gacgatgacg
      301 ccagacctgg acgtggacgc cctgctgtgc tgttggcaga aggcgtcagc tgccctcatc
      361 gaagccgaat gccgcgaccc ctacagcgct gaggtcgacg acatcgccga cgaggtgatt
      421 gcggccagac tcgccctaac ccaagccggc gtgcgagaca tcgccgccct tgctgaaagt
      481 cggcatcacc ccgcgcagcc aaccggcgtc gccatcggtt tctgacgaga cttgacgccg
      541 ctcctggcta cgcgggcgtc ggtgtgtact cgccgacgcg acatacgact tgcctgcggt
      601 ggccgcagtt ccaccacagc acgccgggaa ccttggtcgc aaggagaggc cgaccggacc
      661 cccttgccga ctacacgccc gaaagatacc cagaccacgc ttccacgggt gaaccctagg
      721 tggagaggaa ggtcggacgc cggcggcggg gttgctaccg agaatcctga gagccggcct
      781 cttgatgtgg cctgaggctc gcagcactgt ttgttaacgc tttggatatc cgccattgac
      841 gtagccgcca gacttcgttc ttgggcaacc gatatggtcg tcgcgtggtt tcgaacgcac
      901 ggcccagaca cgcgcggggg ccacgtcacg tcagccgacc cagacatgaa cgggtgcgaa
      961 agcacgctgc cggtccgcga ctgtcgtggg cgaaggttgg gtcctcggga attgtcgcga
     1021 gcctgctggt ctggggtgtc gcttcgacgc ttggatccca cgggacgcac gccacccgtg
     1081 caaccgctct cgccagcgcg ttcggctcgt cggcgtccgg ctatgtgtcg ccgtcgtccg
     1141 actcggagcc cgtcgaacta gccgctgcga tgcctgtcgt cccgggtctc gcacaggcgg
     1201 cgattcgcca gcccgcgtcg gtcggttatg cgacgatcgc cccagtcggt ggagcagcgg
     1261 cgcgcgccgt cggagtggcc gcgcagccgg cggccctcga agtcacgacc attccgcgac
     1321 tcgcaagcgt cgcataccag cgcgcggcaa cgacagaggc gcacgatgcc tcatcgtgcg
     1381 gggtcagttg ggagattctg gcaggcatag ggctggtgga gtccgaccac ggccgcagcg
     1441 gcgggtcagc gagtccgagc tggtcgggcg tcgcaagtcc cgcgatcttc ggcccggtgc
     1501 tcaacggcca ggacggattt cctgtgatca aggacacgga tcatggggtg ctcgacggcg
     1561 acccgttgtt cgatcgcgcc gtcgggccta tgcagttcct gccagccacg tggcgcgaat
     1621 acgacaccgc gacgagcggg tcggcagcgc cgaatccaga gaacatctcg gacgccgcat
     1681 ttgctgccgc ccgttacctc tgtgcgtctg gtgctgattt gcgtacgtca accggtctag
     1741 tcgacgccat ctacgggtac aaccattcgt tcgcctacgt cacgaacgtc gtcaccgccg
     1801 cgcagcgata cgcggaaggc actttgcagg gggcggcggg tgcactggca gaactacccg
     1861 cgcttctctc cgggcaaccc actgcgagct ttgtgacgga cccggtcgtc ttggcgtcag
     1921 gaacgccggg aacgtcgtct ggatcgcctg cgagcgggac gtcggccggt acatcgccga
     1981 gcacgacgcg ttccgccatc gtcggtgctg cgtcgccgga cccggatcca acggtggatc
     2041 cgacctccga tcccatccag gacccgaccc ccgcgccgac cgatagttcg ccgtccgact
     2101 cgtcgtcgac gtcagattcg ccggcccccg atactacgga ctcgcccgcg ccggactcgt
     2161 ccacatcgga tccgtcggca acggactcag cctcgacgtc ggattccgac tcgccacccg
     2221 cagacacttc tgcaccgggt acctctgcgc cggatacgtc gagccccaca ccatcgctgc
     2281 cgccgacacc gacaccgaca ccgacaatca cacccacgcc gacgctcaca ccgacccagc
     2341 ccacgacgac gaccccaaca ccgccaccgg ccacgccggg cggaagcgca agacctggac
     2401 cgacgaacac cggtgtgccg gccggtacgc agttgacggt ctacaacggc gacttgacga
     2461 tcacgacgcc cggcgccacc tacgcggacc tcgacatcca cggcttcatc agggtcgagg
     2521 cgccagatgt cacgatcaaa gactcgatca tccgcggcgg cccggcgacg tccaataccg
     2581 caatcattta tgacctgtca gatgctgcta ccaacctggt tgtcgaggat tccgagatca
     2641 tgcctgcgga tccatcggta ttcatcgacg gcatcgacgg ctggaactac accgcattgc
     2701 gcttgaacat tcacggcact gtcgacggcg cgaaaatgtt cggaaccaat gcgacgatcg
     2761 aacactcctg gatccacgac cttgtgacct acgcgcacga tccatctcag aacggcgggc
     2821 agacgcataa cgacgatgtg cagatcttgt cgggatccaa tctgcagata ctgggcaacg
     2881 acctcgaggg cggcagcaac agcgcgatcc aattat
//
LOCUS       DATCBJ010000074         2003 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_470383, whole genome shotgun sequence.
ACCESSION   DATCBJ010000074 DATCBJ010000000
VERSION     DATCBJ010000074.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2003)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2003)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2003
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_470383"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1973)
                     /locus_tag="VII50_01600"
     CDS             complement(<1..1973)
                     /locus_tag="VII50_01600"
                     /inference="COORDINATES: protein motif:HMM:NF012271.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HEY5049565.1"
                     /translation="MRKVLSSVLVGVTSLALLSTTVIAAAAATTTAGSTCPAIDAPKS
                     MNDMPASADCPTPGALHANLTPLVTPVAPTSPIASPPLPFDRTNGDLLAITKISGTND
                     IAFGGNFTLVYTPDLVSHPAVNFAVVDETTGAILYAGNPSNSVTPSDNYVRAIASLNG
                     VIYVGGDFDGWDGAARTHVAMLSPTGNPAPAAWAVNSSWHPSPGGDIRGLAVDSNAVY
                     MAGDSSILTAVNSTSGATIWSHSGTGGSMHAVFLDQGALFVGGLFATYNGYAQNGLVK
                     VSTVDGSVITAFHTDLRPNTGVGQYGQYDGEDVLALAFAPNSTTQILVGVGGHAPPGD
                     SSNETILFNITTGQRIWKYSTLGDSQAVGAVGDTVVSGYHNNTASAGNPLSANYFGIQ
                     LEGSNGTPTTWDPQINGNQANADGGNNGVQAMYVDPVNDILFLGGAFEHWNGTTGLTH
                     ASLIAFSFTPSTATAPGSPTGVTAVAGNGNAYVSFTAPVSNGGSPITGYTVKSSPGGF
                     TATAPTATPVTVSGLTNGVAYTFTATATNTVGTSAPSTASTPVSPASGGGSSNSAEGG
                     TSGTSVTATNSGGASGNPFSVVSKGTGATLVYSTAADAHGALGYALTGAAGTATDMGW
                     TGYNATSMATRFYYNPGPTLPSTLLRLADIRNAS"
BASE COUNT          347 a          598 c          712 g          346 t
ORIGIN      
        1 gatgcgttgc ggatgtccgc caaccgcaac aacgtcgaag gcagcgtcgg acccgggttg
       61 tagtagaacc gcgtcgccat cgacgtggcg ttgtacccgg tccaacccat gtcagttgcg
      121 gtgccggccg cgcccgtcag ggcgtacccg agcgcgccat gcgcgtcggc agccgtcgag
      181 tacaccagcg tggcgcccgt gcccttgctg accaccgaga acgggttacc cgaggcgcca
      241 cctgaattcg tagcggtcac ggaggtaccc gacgtgccac cctcagccga gttggacgac
      301 ccgccgccgc tcgcggggct caccggcgtc gaagccgtgg agggcgcgga ggtaccgacg
      361 gtgttcgtcg cggtcgcggt gaaggtgtaa gcgactccgt tcgtcaagcc ggacaccgtc
      421 accggggtag ccgtcggagc cgtcgcggtg aatccacctg gcgacgactt cacggtgtag
      481 ccggtaatcg ggctgccgcc gttcgagacc ggcgcggtga acgaaacgta cgcgttcccg
      541 ttgccagcga cagcggtgac cccggtcggc gaacccggtg ccgtggccgt cgagggcgtg
      601 aacgagaagg cgatcaacga ggcatgggtc aggccagtcg tgccgttcca gtgctcgaag
      661 gcgcccccga ggaaaaggat gtcgttgacc gggtcgacgt acatcgcctg cacaccgttg
      721 ttgccgccgt cggcgttagc ctggttcccg ttgatctgcg gatcccacgt cgtcggggtg
      781 ccgttgctgc cttcgagctg gatgccgaag tagttcgcgg acagcgggtt gcccgcggag
      841 gcggtgttgt tgtgataacc cgagacgacc gtgtcaccga cggcgcccac cgcctgagag
      901 tcaccgagcg tgctgtattt ccagattcgc tggccagtcg tgatgttgaa gaggatggtc
      961 tcgttcgacg agtcgccggg cggggcgtgg cccccgacgc ccacgagaat ctgggttgtg
     1021 ctgttgggtg cgaacgcgag cgcaaggacg tcttcaccgt cgtactggcc atattggcca
     1081 acgccagtgt tgggtcgcag gtcggtgtgg aacgcggtga tgaccgagcc gtcgacggtc
     1141 gagaccttga cgaggccgtt ctgggcatac ccgttgtacg tcgcgaacaa gccgcccacg
     1201 aacaacgcgc cctggtccaa gaagacggca tgcatcgaac caccggtgcc cgaatgcgac
     1261 cagatggtgg ccccgctcgt ggagttaaca gccgtgagga tgctggagtc gcccgccatg
     1321 tacacagcgt tgctgtccac cgctaagcca cgaatgtcac cgccgggtga cgggtgccag
     1381 cttgagttga cagcccaggc tgctggagcg gggttgccgg taggcgacag catggcgaca
     1441 tgagtgcgag ctgcgccgtc ccagccgtcg aagtcgccgc caacgtagat gacgccgttc
     1501 aggctggcga tggcacggac atagttgtcg ctgggggtca cgctgttgga cgggttgccg
     1561 gcgtaaagaa ttgcgccggt tgtctcgtcg acgacggcaa agttgacggc cgggtgagac
     1621 acgaggtccg gggtgtagac cagagtgaag ttgccgccga acgcgatgtc gttggtgccg
     1681 ctgatcttgg tgatcgcgag gaggtcaccg ttggtgcggt cgaagggcag cggcggcgag
     1741 gcaatgggcg aggtcggcgc caccggggtg acgagcggtg tcaaatttgc gtggagggcg
     1801 cctggcgtcg ggcagtccgc ggacgccggc atgtcgttca tcgacttcgg ggcatcgatg
     1861 gcagggcagg tcgatcctgc ggtagtcgtc gccgcagcag ccgcaatgac cgtagttgag
     1921 agcagcgcga gcgatgtcac gccgaccaga acagacgaaa gtaccttgcg cattacaatt
     1981 cccccaggga atgactcttg ccc
//
LOCUS       DATCBJ010000075         4925 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_212373, whole genome shotgun sequence.
ACCESSION   DATCBJ010000075 DATCBJ010000000
VERSION     DATCBJ010000075.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 4925)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4925)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4925
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_212373"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..845
                     /locus_tag="VII50_01605"
     CDS             <1..845
                     /locus_tag="VII50_01605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004358 - glutamate N-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="bifunctional ornithine
                     acetyltransferase/N-acetylglutamate synthase"
                     /protein_id="HEY5049566.1"
                     /translation="AVCSTGLIGEYLPIDKLLTGVHAAHAALDADGGGDAARAIMTTD
                     TVPKEATVSSPAGWTVGGMAKGAAMLAPGLATMLVVLTTDAAADAATLDQALRAATAK
                     TFDRLDSDGCMSTNDTVLLMASGASGVEADGHELAAAVTQVCAALADKLIDDCEGASK
                     VIEIEVLGAVNESEAVDVARAVSRSNLLKCAIHGEDPNWGRILSALGTTSATFDDDGV
                     DVAINGVWVCRASTIGDDRTLVDMSARRVIITVDLHAGAAAAKLLTTDLTAEYVRENS
                     AYST"
     gene            842..1750
                     /gene="argB"
                     /locus_tag="VII50_01610"
     CDS             842..1750
                     /gene="argB"
                     /locus_tag="VII50_01610"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HEY5049567.1"
                     /translation="MSTSRDRESAQEKASTLHEALPYLARFHGRTVVVKYGGHAMTDP
                     ALAASFAADVVFLRYAGLRPVVVHGGGPQINAQLDKLGIEPAFAGGLRVTTPETMDVV
                     RMVLVGQVQRDVVGLLNAHGPFAVGLSGEDGQLFTAVRRPAIVDGEPVDIGLVGDIAA
                     VEPGVVTSLLDDGRIPVVSSVARGFGDDGEPCVYNVNADTAAAALAVALKAEKLVVLT
                     DVEGLYADWPNSDAVISALSADDLEVLLPSLSAGMVPKMEACLRAVRGGVASAHVLDG
                     RVPHAVLLEIFTDEGIGTMVKAQVSA"
     gene            1747..2937
                     /locus_tag="VII50_01615"
     CDS             1747..2937
                     /locus_tag="VII50_01615"
                     /EC_number="2.6.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006525 - arginine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine transaminase"
                     /protein_id="HEY5049568.1"
                     /translation="MSNADLLGRFDAVMMPTYAPAVPLVRGEGCTVWDADGRAYLDLI
                     AGIAVSSLGHAHPAVVEAVSTQVATIAHTSNLFCHEPGINLAQKLVSLLGGNARAFFT
                     NSGTEATEAAYKLVRRYAGPNRSYVVAAERSFHGRTMGALALTGKASIRDPFGPYGAD
                     VVFVPYGDTEALAAAVTDRTAALFLEPLLGEGGVIAPPAGYLRAAREICDATGAVFVL
                     DEIQSGIGRTGAWFLHETEGIRPDVLTLAKGLGGGLPIGVCLAIGEFGEVFAKGDHGS
                     TFGGNPIACAAALAVIDTIERHDLLAHVTKVGAHFADGLGAVEHPALVGIRGRGLWLA
                     LELAAPNAPAVEKASRAGGFLVNAVQPDAIRIAPPLILTIEEADRFVAALPTILDAAT
                     GVQP"
     gene            2934..3863
                     /gene="argF"
                     /locus_tag="VII50_01620"
     CDS             2934..3863
                     /gene="argF"
                     /locus_tag="VII50_01620"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HEY5049569.1"
                     /translation="MTTQPRHFLIDDDLDPVEVVAILDQADQLKKDRLAARPLAGPRS
                     VAVLFEKQSTRTRVSFEVGIAELGGHPLIIDARTSQLVRGEPIEDTGRVLSRYVSAIV
                     LRTFGHDRIEALAASSSVPVVNALSDLAHPCQWLADLMTIREHRGEPAGQTITYVGDG
                     NNVAHSILLGGALAGMHVRVASPSGYEPLPEVVQRATAIGERTGGTVLVTPDAADAAA
                     GADVLYTDVWASMGQESESDARSLVFAPYQIDDRLVAFAADDVMVLHCLPAHRGEEIA
                     ASVIDGPHSFVFDQAENRLHAQKALLVFLLEQT"
     gene            3860..4426
                     /locus_tag="VII50_01625"
     CDS             3860..4426
                     /locus_tag="VII50_01625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006525 - arginine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HEY5049570.1"
                     /translation="MSAARQRAAAGPTEGRRNDAPATKAARHARIIDLLAWTPVHSQS
                     ELVELLAAEGLSITQATLSRDLDEIGALRLRSAGGGFVYVVPRDGDDAVSRPTSDRVQ
                     AASAAHARLIRMAGELLVSVRASGNIVVARTPPGGAHLLAGAIDHADLATVIGTVAGD
                     DTVLCVCRDPDGGADVAEQLLHLSDRKS"
     gene            4472..>4925
                     /locus_tag="VII50_01630"
     CDS             4472..>4925
                     /locus_tag="VII50_01630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase domain-containing
                     protein"
                     /protein_id="HEY5049571.1"
                     /translation="MTERVVLAYSGGLDTSVCIGWIAAETGAEVIAVAADVGQGGEDM
                     NVVRERALACGAVEAIVIDAKDEFADDYCLPALKANALYMERYPLVSALSRPVINKHL
                     VAAAHQFGATRVAHGSTGKGNDQVRFEVGIGALDPNLTVLAPVRDSGMT"
BASE COUNT          793 a         1600 c         1694 g          838 t
ORIGIN      
        1 tcgcggtctg ctcgaccggc ctgatcggtg agtatctgcc gatcgacaag ctgctcaccg
       61 gcgtccatgc ggcccacgct gcgctcgacg cggacggcgg cggcgacgcc gccagggcca
      121 tcatgacaac cgacacggtg cccaaagagg cgacggtgag ctcgccggcg gggtggactg
      181 tcggtggcat ggccaagggc gcggcgatgc ttgctccagg gcttgccacg atgcttgtgg
      241 tactcacaac tgacgccgct gcggacgctg cgacgctcga ccaggccctg cgtgccgcaa
      301 ccgcgaagac cttcgaccgg ctcgactccg atggctgcat gtcgaccaac gacaccgtgt
      361 tgctgatggc ctcgggtgcc tcaggagtcg aagccgacgg ccatgaactc gccgccgccg
      421 tgacacaggt gtgcgcggcg ctcgccgaca agctcatcga tgactgcgag ggggcgagca
      481 aagtcatcga gatcgaggtt ctcggtgccg tgaacgagtc tgaggccgtc gacgtcgccc
      541 gcgcggtctc gcgcagcaac ctgttgaagt gcgcgatcca cggcgaggac cctaactggg
      601 ggaggattct ttcggctctc ggtaccacct ctgcgacgtt tgacgacgac ggcgtcgacg
      661 tcgcgatcaa cggggtctgg gtctgccgcg cctcgacaat cggcgacgac cgcacgctgg
      721 ttgacatgtc ggcgcgtcgc gtcattatca cggtcgacct gcatgcgggc gccgctgccg
      781 ccaagctcct cacgaccgac ctgacagcgg agtacgtgcg ggagaattcg gcatactcga
      841 catgagtacc agccgggacc gtgagtccgc ccaggagaag gcctcaacgc tgcacgaggc
      901 gctgccgtac cttgcccgct tccacggccg cacggtggtg gtgaagtacg gcggacacgc
      961 aatgaccgat ccggcactcg ccgcatcatt cgccgcggac gtcgttttct tgcgctacgc
     1021 aggtctacgg cctgtcgttg tccatggtgg cggcccgcag atcaacgcac agctcgacaa
     1081 gctcgggatc gaacccgcgt ttgccggcgg gctccgggtc acgacacccg aaacgatgga
     1141 cgtggtccgc atggtgctcg tcggtcaggt gcagcgcgac gtcgtcgggt tgctcaacgc
     1201 acacggcccc ttcgcggttg ggctcagtgg cgaggatggg caactgttca ccgcggtccg
     1261 ccggccggcc atcgtcgacg gcgaacccgt agacatcggc cttgttggtg acatcgcggc
     1321 cgtcgaacct ggtgttgtca cgagcctgct cgacgacggg cgcatccccg ttgtctccag
     1381 cgtggcccgc ggcttcggtg acgacggaga accgtgcgtc tacaacgtca atgcggacac
     1441 ggcggcggcg gcgctcgcgg tcgcgctaaa agccgagaag cttgtggtgc tgaccgacgt
     1501 cgagggcctc tacgcggatt ggccgaacag cgacgcggtg atcagcgcgt tgagcgccga
     1561 cgacctcgag gtcctgctgc cgagcctgtc ggcaggcatg gtgccgaaga tggaagcgtg
     1621 cctgcgcgcc gtcaggggcg gtgtcgcgag cgcccacgtt ctcgatggac gggtacccca
     1681 cgccgtcctg ctcgaaatct tcaccgatga agggatcggc acgatggtca aggcgcaggt
     1741 gtcggcatga gtaacgccga cctgttgggc cgcttcgacg cggtcatgat gccgacgtat
     1801 gcacccgcgg tgccgctcgt gcgtggcgag ggttgcacgg tatgggacgc cgacgggcgc
     1861 gcgtacctcg acctgatcgc gggcattgcg gtgtcgtcct tgggccacgc gcacccggcc
     1921 gtcgtcgagg cggtgtccac ccaggtcgcc acgattgcgc acaccagcaa cctcttctgc
     1981 cacgagccgg ggatcaacct tgcccagaag ctcgtgagct tgctcggcgg caacgcccgt
     2041 gcgttcttca ccaacagcgg caccgaggca accgaagccg cctacaagct ggtacggcgc
     2101 tacgccggtc ccaaccgcag ctatgtcgta gcggcggagc gaagctttca cggtcggacc
     2161 atgggcgctc tcgcactgac cggcaaggca tcgattcgtg acccgttcgg gccttacggc
     2221 gccgacgtcg tgttcgttcc ctacggcgac accgaggcgc tggcggccgc agttacagat
     2281 cgaactgcgg cgctgtttct cgagccgttg ctgggagaag gcggcgtgat cgccccgcct
     2341 gccggttatc tgcgggcggc ccgcgagatc tgcgacgcca ccggtgccgt gttcgtcttg
     2401 gatgaaatcc agtccggcat tggacgtacc ggcgcctggt tcctgcatga gaccgagggg
     2461 atccggccgg acgtgttgac tctcgcgaag ggtttgggtg gcggattgcc gatcggagtg
     2521 tgtctcgcga tcggtgagtt cggcgaggtg ttcgcgaagg gcgaccacgg cagcaccttc
     2581 ggtggcaatc cgattgcctg cgccgcggca ctggccgtga tcgacacgat cgagcggcac
     2641 gatctcctcg cccacgtgac caaggtcggc gctcatttcg ccgacgggct aggcgctgtc
     2701 gagcaccccg ctcttgtggg catccgcggt cgcggtttgt ggctcgctct cgagttagct
     2761 gcacccaacg cgccggcggt cgagaaggcc agcagggctg gcggatttct ggtgaatgcg
     2821 gtgcaacccg atgccatcag gatcgcgccg ccgctgatcc tgacgattga ggaagccgac
     2881 cggttcgttg ccgcgctgcc gacgattctc gacgccgcga ccggtgtgca accgtgacga
     2941 cgcagccccg acacttcttg atcgacgacg acctcgaccc cgttgaggtg gtggccatcc
     3001 tcgatcaggc ggaccagctc aagaaagaca ggctggctgc gagaccgctt gcaggtccgc
     3061 ggtcggtcgc ggtcttgttc gagaaacagt cgacccggac ccgggtctcg ttcgaggtcg
     3121 gtatcgccga acttgggggg caccccctca tcatcgatgc ccgaacgtcg caactggtcc
     3181 gcggcgaacc gatcgaggac accggccggg tgctatcgcg gtacgtgtcg gcgatcgtat
     3241 tgcgaacgtt cgggcacgat cggatcgagg cactcgcggc gagttcgagc gttccggtcg
     3301 tgaacgcgct gtccgacctt gcgcacccgt gccaatggct ggccgacctg atgacgatcc
     3361 gggaacaccg tggcgagccc gctggtcaga cgatcaccta cgtcggggac ggcaacaatg
     3421 tcgcgcattc gatcctgctc ggtggggcgc ttgcgggcat gcacgtacgg gtggcatcgc
     3481 cgtccggata cgagccattg cccgaggtcg tccaacgagc aacggcgatt ggtgaacgca
     3541 caggcggcac cgtgctggtg acgccggacg ccgctgatgc cgccgccggt gccgacgtcc
     3601 tgtacacgga cgtctgggcg tcgatgggcc aggagagtga gtccgacgca cgcagcctgg
     3661 tattcgctcc gtaccagatc gatgatcgcc tcgtcgcgtt cgcggcggac gacgtgatgg
     3721 tgttgcactg ccttccggcg catcgcggcg aggagatcgc cgcgtccgtc atcgacgggc
     3781 cgcattcgtt cgtgttcgac caggcggaga accggctgca cgcccagaag gcgctgttgg
     3841 tcttcctgct ggagcagacg tgagcgcggc ccgtcaacgc gccgcggccg gcccgaccga
     3901 aggacggcga aacgacgctc ctgccacgaa ggcagcgcgg cacgcccgga tcatcgatct
     3961 gctcgcctgg acgccagtgc attcacagtc agagctcgtc gagctgctgg cggccgaggg
     4021 tctgtcgatc acgcaggcga ccctgtcgcg cgacctcgac gagatcggag ctctgcggct
     4081 gcgcagcgct ggtggcggct tcgtctacgt ggtgccgcgt gatggcgacg atgcggtgag
     4141 ccggccgacg tccgaccggg tgcaggccgc gtcggctgcc cacgcacggc tgatccggat
     4201 ggcgggggag ctgttggtct cggtacgcgc gtccggcaac atcgtggtgg ctcgcacccc
     4261 accgggcggt gcgcatctgt tggccggcgc catcgaccac gccgatttgg caaccgtcat
     4321 cggtacggtg gccggcgacg acaccgtgtt gtgcgtatgc cgcgaccctg acggcggcgc
     4381 cgatgtggcc gagcagttgc tgcacttgtc cgaccgcaag tcctaaccgc atgaccaaat
     4441 cgatcgcacc gactaccact ggagaagttt cgtgacagag cgcgtcgtac tcgcctattc
     4501 aggaggtctc gacacctcgg tgtgcattgg ctggatcgcg gccgagaccg gggcggaggt
     4561 catcgcggtc gcggccgatg tcggccaggg cggcgaagac atgaacgtcg tgcgcgagcg
     4621 agcacttgcg tgcggcgcgg tggaggcgat cgtcatcgac gccaaggatg agtttgccga
     4681 cgactactgc ctgcccgcgc tgaaggccaa cgcgctgtac atggagcgct acccgctggt
     4741 ctcggcgctg tcgcggccag tcatcaacaa gcacctcgtc gcggccgcgc accagttcgg
     4801 cgcgaccagg gttgcgcacg gcagcaccgg taagggcaac gaccaggtca ggttcgaggt
     4861 cgggatcggc gcgctcgatc caaacctgac ggtgctcgcg ccggtgcgcg actctggcat
     4921 gaccc
//
LOCUS       DATCBJ010000076         3295 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_175962, whole genome shotgun sequence.
ACCESSION   DATCBJ010000076 DATCBJ010000000
VERSION     DATCBJ010000076.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 3295)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3295)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3295
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_175962"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..649
                     /locus_tag="VII50_01635"
     CDS             <1..649
                     /locus_tag="VII50_01635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5049572.1"
                     /translation="LTTTGQLPGWLDWFSTIVTIAGVAGILLVGVRRQVRNWRQAGGE
                     RRQQLKWVAFGGAVSVVWLAGTPVVGNFFFVGLAALPVSMGIAILKYRLYDIDRIISR
                     SLSYALVTGLLVGVYVGMIALTTLVIPLSSSVAVAASTLAAVALFTPLRRRVQQRVDR
                     RFNRATYDSAAMVSAFTSRLRDAVQLETVRTDLLTLVSAAVEPTHVALWIRPISR"
     gene            909..1109
                     /locus_tag="VII50_01640"
     CDS             909..1109
                     /locus_tag="VII50_01640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019257861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEY5049573.1"
                     /translation="MQNKVRQLRTAGGLSQAELGTKLGVSRQTINAIEAGRYLPSLPL
                     ALGLARFFSSTVEDLFFDADSR"
     gene            1115..1513
                     /locus_tag="VII50_01645"
     CDS             1115..1513
                     /locus_tag="VII50_01645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5049574.1"
                     /translation="MAEQLRAAARKHATLATLAILGGAITAAIWAGGDHGWAVGVGAF
                     YLVCCAALFMWSRGDGDVAAILRLSGDERQAMLDLRATAATGVATIVFCLAGAIVNLA
                     RGGSGDPWTLICAVAGFAYIVALAWSRHRH"
     gene            2011..2676
                     /locus_tag="VII50_01650"
     CDS             2011..2676
                     /locus_tag="VII50_01650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5049575.1"
                     /translation="MTSVLCDWPGTNGAGIRLFGQPAGQATGGVALVILVTAQSVNVR
                     AATGSGTTYAQREFNGTGVTNFDAAKGGSINTPLTEDPAAGTHPGTIGAARSINLTID
                     CSGQQPGTTSLTVSGNTAGGAVTGGVSSARVDCASYAQGLTADVTGLTTVGGKSVLVI
                     VGVTWAGITVAVDGHFYTNSAVTTGTASATGGQWNGDVTDQSTGTTLHVAGDATCGSS
                     TKT"
     gene            complement(3036..>3295)
                     /locus_tag="VII50_01655"
     CDS             complement(3036..>3295)
                     /locus_tag="VII50_01655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5049576.1"
                     /translation="TLGTVIEVDLTVTPPATVGAATSSNEAVVTPISGQGQNTPTSRT
                     VFRADAAGTANLFAMINAACAPNAGCGAAAGFSYLLTVVPR"
BASE COUNT          560 a         1072 c         1074 g          589 t
ORIGIN      
        1 cctcaccact acgggtcagc tgccgggctg gctggactgg ttcagcacga tcgtcaccat
       61 cgcgggagtc gcggggatct tgctggtggg cgtccggcgc caggtgcgga actggcggca
      121 ggcgggaggc gagcgccgcc agcagctgaa gtgggtggcg ttcggcggcg cggtcagtgt
      181 cgtctggctc gccggtactc ccgtcgtcgg taactttttc tttgtcgggc tcgcggcgct
      241 gccggtcagc atgggcatcg cgatcttgaa ataccggctg tatgacatcg accggatcat
      301 cagccgcagc ctgtcatatg cgctcgtcac tggcctgctg gtcggtgtct acgtggggat
      361 gatcgcgctg accaccctcg tcatcccgtt gtcgtcttcg gtggcggtcg ccgcatcgac
      421 gctggccgcc gtcgcgctgt tcaccccgct gcgccgccgg gtccagcaac gggtcgaccg
      481 acgattcaac cgggccactt acgactccgc cgcgatggtg tccgcgttca cctcgcgcct
      541 gcgcgatgct gtccaactcg agaccgtgcg gaccgacctg ctcaccctgg tcagcgccgc
      601 ggttgaacca acgcatgtgg cgttgtggat ccggccgatc agtcgctgag gcgactgtcg
      661 catcgtcctc aaccttcgtc atgttgaccg ccggtcgggc gccaaagacg atgatgctca
      721 gcaagcaaac cccatcagct ggccttcacg cgatgcggtt gaaaggacag ctaccgacgg
      781 ccctaactga actaccggat ctcggcagga ccaacgtcat agccagaggc ggatgtttct
      841 tgacgatccc cgaaccacgt atggaaagca accttgacac cgattgtatg acaagctagc
      901 tttacactgt gcagaacaag gtacgtcagc ttcgaacggc tggcggacta tcccaagcgg
      961 agctcggtac gaagctcggc gtgtcacggc agaccatcaa cgccatcgag gccggccggt
     1021 acctgccatc gctgccgttg gcgttgggac ttgcgcgctt cttctccagc actgtcgaag
     1081 accttttctt cgacgccgac tcgaggtgac gaccatggcc gagcaactca gggctgcggc
     1141 ccgaaagcac gcaacgttgg cgaccctggc gatccttggg ggcgctatca ccgcggcgat
     1201 ctgggctggc ggtgaccacg gatgggcggt cggcgtcggc gccttctacc tggtgtgttg
     1261 tgctgcgctg ttcatgtggt cacgcggtga cggtgacgtc gccgcgatcc ttcggctctc
     1321 cggagacgag cggcaagcga tgctcgacct gcgcgccaca gctgctactg gggtcgccac
     1381 catcgtgttc tgcctcgcag gcgccatcgt caacctcgca cgtgggggca gcggcgaccc
     1441 gtggacactg atctgcgccg tcgccgggtt cgcctacatc gtcgcgctcg cgtggagccg
     1501 tcaccgccac tgactcccgc gcagcaccat cctcgtcgcc ttgcgtgatt gagccgccgg
     1561 tgtgtcgtgt ccgtcgcctg ggcaggtgcg agtgtgatgt gacgttcggt tgaaggtgac
     1621 gttcggttga aggtgatgtg ccactgagca cggaactcac cctccggcca ttgaccatgt
     1681 gcacggtatg ctggccgccg caggcggcct ggatacagga gccttaccgt gttgaaccgt
     1741 tgggtcgtcg cgatgctagc cgtgactacc gtcgcgctcg ctgcctgcag cggttcgtca
     1801 aggtccgcca caacgggggg tagcgccact gcgaccgcga caccggcccc cgcgggttcg
     1861 ccgacggcga ctgggtcgat cgtcggtggt tcgtcaaggt ccgccgcgac gggggctagc
     1921 gccactgcga ccgcgacacc ggcccccgcg ggcccgccga cggcgaccgg gtcgatcgag
     1981 ggagatacca acgtcggggg cagtgtcaag gtgacgtcgg tcctatgcga ttggccgggg
     2041 acgaacgggg ccggcatccg gctgtttggt caaccggcgg ggcaggccac gggcggtgtc
     2101 gcgctggtca tcctcgttac cgcgcaatcg gtcaacgtca gggctgccac cggttctggg
     2161 accacctacg cgcaacgcga attcaacgga accggggtta ctaacttcga cgcggccaag
     2221 ggcgggagca tcaacacacc gctcaccgag gaccctgcgg cgggtaccca ccccggcacc
     2281 atcggcgcgg cgaggtcgat caacttgacg attgactgtt cgggtcagca accaggcacg
     2341 acgtcattga cggtgtcggg aaacactgca ggcggggcgg tgaccggagg ggtctcctcc
     2401 gcgcgcgtgg actgtgcgag ctacgcccag gggctgaccg ctgacgtcac cggtctcacc
     2461 acggtcggtg ggaagtcggt tctggtcatc gtcggcgtca catgggcagg catcaccgtc
     2521 gccgtcgacg gccatttcta tacgaactct gccgtcacaa ccggaaccgc cagcgcgact
     2581 ggcggccaat ggaacggtga tgtgaccgac cagagcactg gcaccacgct gcacgtagcc
     2641 ggtgatgcta cctgcgggag ctcgacaaaa acctgacctt cgccggcgct ggtggccaac
     2701 aacgttcgaa ggcgccatcg tgtgcagctc caccgcaagt gacccttggc gggctcttac
     2761 gacaggctca cacgatcgcc cctcaaccct gaggaacccg gccggagttg caccactcga
     2821 cgcgacatca ccaccatgcg gtccccaacc aatcccatca gatccgagct gggccacatc
     2881 cgcacatcga cacgatcgag cctgcaggaa cgaccgggtc agcggcagaa gcaacgtttg
     2941 tgagctgcgc tcgtgaactc gactgctgta tcaaggacgc gacgcaacgc cgcggcgacc
     3001 atcatcttgt gcaggctaaa ggcgcggtca aacgctcatc gcggcacgac tgttaacagg
     3061 tacgagaacc cggccgccgc gccgcatccg gcgttcggcg cgcatgcggc gttgatcatc
     3121 gcgaacagat tcgctgtgcc ggcggcatcc gcgcggaaga ccgttcgact ggtcggtgtg
     3181 ttctgtccct ggccgctgat cggggtgacg acagcttcgt tgctggaagt tgcggcaccg
     3241 accgtggcgg gcggcgtaac agtcaggtca acctcgatga cagtaccgag tgtga
//
LOCUS       DATCBJ010000077         2693 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_248724, whole genome shotgun sequence.
ACCESSION   DATCBJ010000077 DATCBJ010000000
VERSION     DATCBJ010000077.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2693)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2693)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2693
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_248724"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..506
                     /locus_tag="VII50_01660"
     CDS             <1..506
                     /locus_tag="VII50_01660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5049577.1"
                     /translation="VFLGNIGIKPLTGPLLRRFGFRTVLVHAAVWSAATIALCGLVTA
                     HTPLPVIALILLASGVFRSIGFTAYNTISFVDIAPEAIGDANTLTTTIQQLTMGMGVV
                     AGALALRAGGPIAKLLGVTAGARGAFAIAFCLIAVVALLAVVESALLPRDAGAAATAP
                     PMAEQPT"
     gene            complement(484..>2693)
                     /locus_tag="VII50_01665"
     CDS             complement(484..>2693)
                     /locus_tag="VII50_01665"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEY5049578.1"
                     /translation="QETEADAVRVAVERALETFGAELGALVRDQAILACVGVGRNEPP
                     VEALTRAVAGTTVIDVAGLGVLHAVAVHLYDDRSGHLVVARADEEFTGEERQLLLSMA
                     SVLGLALRSLRTLQVERTLRNEREQEATERLRLLETLGQRQRLLESLLTIQRAISHRA
                     PLQTVLDAVTTGAAQLLNDAFVALVLSEPLAGRNRIVSSAAPAHDTDDHDALVMAAAA
                     DSVAIDGVVTRVSSEYDAVGRTALAAPVHIEGETIGSLVTVVLPETPDVDDRREMLAA
                     FAEQASLALTDARTVEAMRDAYHDSVTGLPNRSLFLDRLNQALQNGARMGTEVTVLFI
                     DLDRFKDVNDSLGHAAGDELLAWVAGRVREALRDDDTAARLGGDEFAVLLERTVGSDA
                     GQIVAGRIARALLEPIRLVGREVFAFASIGIASSDGSVRTADELLRNADVAMYRAKRS
                     GARAALVYDPSMHTETMDVLELGSDLYHAVARGELRLQYQPVVNLRTGKAVAAEALVR
                     WDHPARGLIAPNAFIPIAERNGVIVEIGQWVLGSGCTQAAAWRASSLPDLRISVNVSA
                     RQLDDLGFADMVTEVLRQTGLPAEALTLEVTESVLMHDPLRIIDRLSPLKALGVRLAI
                     DDFGTGYSSLASLQHFPADQLKIDKAFIDTIETSAEGSAIVRTVVELARTLRMETVAE
                     GIETMQQFASLRELSCDLGQGYHFARPLAAEAVPAFFATSMGIGDLLTLAARP"
BASE COUNT          462 a          925 c          846 g          460 t
ORIGIN      
        1 cggtgttcct cggcaatatc ggcatcaaac cgctcaccgg cccactgttg cgccggttcg
       61 ggttccggac ggtgctcgtg catgcagcag tgtggagcgc ggcgactatc gccttatgcg
      121 gcctcgtcac cgcccacact ccgctgccag tgattgctct catcctgctc gcgagtggag
      181 tctttcgctc cattggattc acggcgtaca acaccatcag cttcgtcgat atcgcgccgg
      241 aagctattgg cgacgccaat acgctgacaa cgaccatcca gcaactaacg atgggaatgg
      301 gcgtcgtcgc gggcgcgctg gctctacgcg cgggtggccc gattgccaag ctcctcggtg
      361 tcactgcggg cgcgcgcggt gcattcgcga tcgcgttttg cctcatcgcg gtcgtcgcgc
      421 tgctcgcggt ggtcgagtct gcactgctgc cgcgcgacgc cggcgcagcc gccaccgccc
      481 cgcctatggc cgagcagcca acgtgagcaa gtcaccgatc cccatagagg tggcgaagaa
      541 ggccggtacg gcctcggcag cgagtggccg cgcgaagtgg tagccctgac cgagatcgca
      601 cgacagttcc cgcagactgg cgaactgctg catcgtctcg atgccttcgg cgacagtctc
      661 catgcgcagt gtgcgggcca gttccacgac ggtccgcacg atcgccgagc cttcggcaga
      721 ggtctcgatg gtgtcgatga acgccttgtc gatcttgagc tggtcggccg gaaaatgctg
      781 cagggaggcg agcgatgagt aaccggtgcc aaagtcgtcg atcgccaacc gaacgcctag
      841 tgcctttagc gggctgagtc ggtcgatgat ccggagcgga tcgtgcatca gcaccgactc
      901 ggtgacctcc aatgtcagcg cctcagcggg caggccggtc tgccgtagca cctcggtcac
      961 catgtcggcg aaccctaggt cgtcgagttg ccgggccgac acgttgacgc tgatccgcag
     1021 gtcgggcagc gacgatgcgc gccacgctgc agcctgcgtg caaccagaac ccaacaccca
     1081 ctggccgatc tcgacaatga cgccgttgcg ctcggcgatc gggatgaagg cgttcggtgc
     1141 aatcaagcct cgggccggat gatcccaccg caccaacgcc tctgctgcga ccgctttacc
     1201 ggtccgcagg ttgaccacag gctggtactg aaggcgcaac tctccgcgcg ccactgcgtg
     1261 gtacagatcg ctgccgagtt ccaacacatc catcgtctcg gtgtgcatgc tcgggtcgta
     1321 gaccagcgca gcgcgggcgc cgctgcgctt cgcgcggtac atcgcgacgt ccgcgttacg
     1381 caagagctca tccgccgtgc gcaccgagcc gtcgctcgag gcgattccga tgctggcgaa
     1441 ggcaaagacc tctcggccga ccagccggat cggctccaga agcgccctcg cgatccggcc
     1501 cgcgacgatc tgaccggcat cggagccaac ggttctctcc agcaggacgg cgaactcgtc
     1561 cccaccgagg cgggcggcgg tgtcatcgtc gcgcaaggcc tcacgcacgc gaccggcaac
     1621 ccaagccagc agctcatcgc ccgccgcatg gcccagactg tcgttgacgt ctttgaaccg
     1681 atccaggtcg atgaagagca ccgtaacttc tgtgcccatc cgagcaccgt tctgcaacgc
     1741 ctgattcaag cgatcgagaa acagcgatcg gttcggaagt cccgtcaccg agtcgtggta
     1801 cgcgtcacgc atcgcctcaa cggtgcgcgc gtcagtcaat gcgaggctcg cctgctcggc
     1861 gaatgccgcg agcatctcgc gccggtcgtc gacatctggc gtctcgggaa gcaccactgt
     1921 cacgaggctg ccgatcgtct ccccttcgat gtgcaccggc gctgccagcg ccgtacgtcc
     1981 gaccgcgtcg tactcgctgc tcaccctggt gaccacgccg tcgatggcga ctgagtcggc
     2041 tgcggccgcc atgacgagcg catcgtgatc gtcggtgtcg tgggccgggg cggccgaaga
     2101 aacgatccga ttccggccgg ccaacggctc gctgagcacc agcgcgacga acgcatcgtt
     2161 cagtagttgg gctgcgccag tcgtgacggc atcgagcacg gtctgcagcg gcgcccgatg
     2221 cgagatggca cgctggatgg tgagcaacga ctcgagcagc cgctgtcgct gaccgagcgt
     2281 ctcgagcagc cgcaaccgtt cggtggcttc ctgttcacgt tcgtttcgca gcgtgcgctc
     2341 gacctgcaac gtacgcaaac tgcgcagcgc cagtccaagg acgctcgcca tgctgagcag
     2401 tagctgacgt tcctcgccgg tgaactcctc gtccgcccgc gcgacgacaa ggtgtccgga
     2461 acggtcgtcg taaaggtgca cggcaacggc gtgcaacact cccagccctg cgacgtcgat
     2521 gacggtcgtg ccggcgacgg ctcgcgtcag cgcctcgacg ggcggctcgt ttcggccgac
     2581 tcccacgcac gccaagatcg cctggtcgcg aaccaacgca ccgagctccg caccgaacgt
     2641 ctcgagtgca cgctcgacgg ccacgcgaac ggcgtccgct tcagtctcct ggg
//
LOCUS       DATCBJ010000078         7591 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_138029, whole genome shotgun sequence.
ACCESSION   DATCBJ010000078 DATCBJ010000000
VERSION     DATCBJ010000078.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 7591)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7591)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7591
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_138029"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..381
                     /locus_tag="VII50_01670"
     CDS             <1..381
                     /locus_tag="VII50_01670"
                     /inference="COORDINATES: protein motif:HMM:NF016047.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding repeat-containing protein"
                     /protein_id="HEY5049579.1"
                     /translation="STATYLGAHPGRAYAIGGPAAAADPAASATVGVDRYATAAAVAT
                     KFFSAPTIVGFATGSNFPDALAGGSLLAKEGAPLLLATAAGLSTPTATYLTSVRSSVV
                     TAQLFGGDTVISGTASTAITNALN"
     gene            complement(362..1801)
                     /locus_tag="VII50_01675"
     CDS             complement(362..1801)
                     /locus_tag="VII50_01675"
                     /inference="COORDINATES: protein motif:HMM:NF028467.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing diguanylate cyclase"
                     /protein_id="HEY5049580.1"
                     /translation="MGRPGFRATQPAPLLASVAVLTVTLALLPWAGRYLGPGLGFLPM
                     MLSAAACFDILTAWLLAGNFLDTGDRRFLVMACAYLWSLLLMGAYAFAFPGVVSSHPP
                     LAVTVSMAPYMFVCWHAGFPVLLGAAWGPWLGPNAIETVRPKRRRLLAAAAGATIAVG
                     AATIAAFAANAHRLPALIHGIDTTQLARVTGPIGLPLVLISLVFCARGIRGATGPERW
                     AIVAILGCLCDLVISYPSHYRYSLGWYAGRTLGMLSAGVVLAAMLASFRRLKATAEFD
                     AAYDHLTGLPNRRSAQDSLNAMFARGRRMHTALALIAFDLDFFKRINDEYGHAAGDAV
                     LRAVGLAVRTAIRPSDIVARIGGEEFLVLLPDATDDDARMVAERLRTALRTLDLIGPD
                     VPVTASFGVARMQDFDLDAEQLLQRADRALYGAKASGRDGIVVAPLVPSGVIEPDDQL
                     PGALVRCAASAGSTNSSSTAKKSPSSTHS"
     gene            1903..2259
                     /locus_tag="VII50_01680"
     CDS             1903..2259
                     /locus_tag="VII50_01680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5049581.1"
                     /translation="MGADFQIVNIIFADMCIGASGDASGLAAALAGLSLAFVLFGGGF
                     TGAATEAGSVAAAGQAGKFGAALGIGLLGLAFCDDRAPAPAIPPAPIDDGDDSGGTYV
                     KPVPECPVEESEGGTC"
     gene            complement(2290..2622)
                     /locus_tag="VII50_01685"
     CDS             complement(2290..2622)
                     /locus_tag="VII50_01685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5049582.1"
                     /translation="MTYGAATPQHVEPGIDACDYANKGSSDPIDIQDLTIQVISLAGC
                     YTQLREADGPGVTVTGIGDEAFGHEIGLDVKLGNKCIEVSGLTHAEFADNYAPDVAMA
                     KIIIAKLG"
     gene            2654..3001
                     /locus_tag="VII50_01690"
     CDS             2654..3001
                     /locus_tag="VII50_01690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5049583.1"
                     /translation="MSEQTSMAPPPAAGAGTVEAAELAAVLALAGALDSTDWLATDAR
                     GDAVGVASSAAVAVAGSAVAGVGVVLDFVVGLHAADDNATTTMAVVADSARTCMAAPY
                     SAARRLALPPNRS"
     gene            3118..5931
                     /locus_tag="VII50_01695"
     CDS             3118..5931
                     /locus_tag="VII50_01695"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HEY5049584.1"
                     /translation="MIESDQLRGADPADLIGRAAERLAIVSALDEAADRTAAVLLTGD
                     AGIGKTAIWESIVEQRRAAGDHVLISRATSAEARLPWVGMTDLLRAMPSTTLQSLPDV
                     QRRALEVVSLQSGSVHGGSSETLDERMVGTALLSALQSATNTAPVLLAIDDLPYLDTA
                     SASAVTFALRRMEGPHPARLLATVRDHDVPLPVLQGLPTDRCSVISIGPLTLGALFDV
                     LQSRRGIRLARPMLLRVYETSGGNPLYALELARALDRLEISPKAGTPLPVPAGLNALV
                     DARVRDLPAEVADVVAATSAAWRFTATDRDADAIELAVAAAMVIVEEPAVVGGARVIR
                     AAHPLLSAAAYNGLTTSRRRQLHARLAAAADDPVERVRHAALAATHPQATLAADLDVG
                     VAAALAAGVPDIAVGLAQLSLEHTTDVALRPARLDRLADAQLRSGDSSGAWQSQSEAI
                     AATEAGAERARRRIRLAEIAVEVTSWPDAERELQVAIDEAAGDPLVLAEALLTLAAVT
                     DDTDVAVTSAQRAVDLLDAQGDPDPMILSGALGQLAGASFRSGYGLDRELFARAIDIE
                     RGHPSRRLSDRADAAYAALLKYADDIDAAEDRLLALLEETRATGDLSSITYALGHLVP
                     IYLWRGQLARGRGYADEHLEVSIQGELQGQETSARYNLGLAMAYQGQLDEASQVLLGL
                     LAAESTDSWIVHRVHAVLGFVALSRNDATGAVEHTDLWHDALVEMHFGEPGYSRGHLD
                     HLCALIGAGRTADAATFCDELSEQAQRSGRESAAAVVSTGRAMLEAHAGRLSNALALS
                     TSALSWYDSSPLRFDRARTLLIAGRISRRAKAKSDARALLTEAEHEFASFGATAWQEQ
                     AAAELARVNVRPSAPAELTETERLVAQLAASGLSNKEVADRTFLAVKTVEANLARAYR
                     KLGIRSRAELGARMGVVPQRGAQ"
     gene            complement(5925..6176)
                     /locus_tag="VII50_01700"
     CDS             complement(5925..6176)
                     /locus_tag="VII50_01700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5049585.1"
                     /translation="MAGFLVERYWPGVTAADVDAVCRRLTELGGADATFVASTLIPAD
                     EVVFFEFRAVDDAGVLELARRADLRCDRLVSAERHPHAY"
     gene            complement(6344..7372)
                     /locus_tag="VII50_01705"
     CDS             complement(6344..7372)
                     /locus_tag="VII50_01705"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEY5049586.1"
                     /translation="MHVGVDSFVSAVTDPVTGREVGPEERIEHLLEEIALADAAGVYS
                     FGIGEHHRVEFYDSAPPVILGAAAARTSRIRLGSAVTVLSAADPVRVFQQFATLDLIS
                     KGRIDLIVGRGSFIEAFPLFGLDLDDYDTLFAEKLDLLLKIRDDVHVTWSGRHRAALT
                     GQGVYPRPVQNPLPIWVGVGGTPESFVRAGLLGLPLMVAIIGGEPRRFAPLVDLYRRA
                     GAEAGHDPEHLQVGLHVFGFVADSVRSASDTIYPGWHQMFTRASQERGFAPPTRAQFD
                     ATSGPDGAFFLGDPDTVATKLRRISAQLGGVDRVSMQMTNPRLAHADLLRGIELLGTK
                     VAPQVADD"
BASE COUNT         1268 a         2557 c         2546 g         1220 t
ORIGIN      
        1 tcgactgcga catacttagg tgcgcatccg ggccgtgcct acgccatcgg cggccccgcc
       61 gctgcggccg acccggcagc ttcggcaacc gtcggcgttg accgatacgc gacggccgca
      121 gccgttgcca cgaagttctt cagtgcaccg accatcgtcg ggttcgcgac cggatccaac
      181 ttccccgacg ccctcgctgg tgggtcgcta cttgcgaagg aaggcgcacc cttgctgctc
      241 gcgaccgcgg ctggcctgtc cacgccgaca gcgacctacc tgacgtccgt ccggtcgtca
      301 gtcgttacgg cacagctgtt cggcggcgac accgtcatat cgggcacggc ctcgaccgca
      361 atcacgaatg cgttgaacta ggggacttct tcgccgtgga ggaggaattc gtcgaacccg
      421 cggatgccgc gcagcggacc agagccccgg gtaactgatc gtcaggttcg atgacgccgc
      481 tgggcacgag cggcgcgacc acgatgccgt cgcgaccgga cgccttcgcc ccgtacagcg
      541 cgcggtcagc ccgctgcagc agctgctcgg cgtcgaggtc gaagtcctgc atgcgcgcga
      601 ccccaaagct cgccgtcacc ggtacatccg ggccaatgag gtcgagcgtg cgcagtgcgg
      661 tgcgcagccg ctcggccacc atgcgcgcat cgtcatcagt cgcgtccggc agcagaacaa
      721 ggaactcctc gccaccgatg cgtgcgacga tgtcactggg acgaatagcg gtgcggacgg
      781 cgagaccgac ggcccgcagg acggcgtcgc ccgcggcatg gccgtactcg tcgttgatcc
      841 gcttgaagaa gtcgaggtcg aacgcgatca gcgccaacgc ggtgtgcatc cggcgaccgc
      901 gcgcgaacat cgcattcaac gaatcctgag cgctgcgccg gttcggcaat ccggtgaggt
      961 ggtcgtaggc cgcgtcgaac tcggccgtcg ccttgagtcg ccgaaacgag gcaagcatcg
     1021 cggccaggac gacgccggcc gacagcatgc ccagcgtgcg gcccgcatac cagccaaggc
     1081 tgtatcggta gtgcgaggga tagctgatga cgaggtcgca aaggcaaccc aggatggcga
     1141 cgatcgccca gcgctcgggt ccggtggcgc cacggatgcc gcgcgcacaa aacaccaggc
     1201 tgatcaagac caggggcagg ccgatcggcc ctgtcactct ggccagctgc gtcgtgtcga
     1261 tgccatgaat cagcgcaggc agccggtgcg cgttcgccgc gaaagcggcg atcgtcgccg
     1321 ctcccaccgc gattgtcgct ccggcggcgg cggcgagtag ccgtcgccgc ttcgggcgca
     1381 ccgtctcgat ggcgttcggt ccaagccacg gaccccaagc agcgccaagc aacaccggga
     1441 agcccgcgtg ccagcagacg aacatgtacg gagccatcga caccgtcacc gcgagcggcg
     1501 gatggctcga gacaacgccc gggaacgcga acgcgtacgc gcccatcagc aggagcgacc
     1561 acaagtacgc gcacgccatg accaggaacc ggcgatcacc ggtatcgagg aagtttccgg
     1621 ccagcagcca ggcagtcaga atgtcgaagc aggcggccgc cgagagcatc atcggcagga
     1681 agcccagccc ggggccgaga taccgaccgg cccacggcag gagcgcaagc gtcactgtta
     1741 ggacggcaac ggacgcgagc agcggggcgg gctgcgtcgc acgaaatcca gggcgaccca
     1801 tgaatctctc atcggcggcc ggccgcgact ccggaggaaa aattacgacg agtcaggaca
     1861 ccgccaacaa atgcgagcct ctattactgc ctcggcaaga cggtgggcgc ggatttccaa
     1921 atcgtcaaca tcatcttcgc ggacatgtgt attggcgctt caggtgacgc gtccggactc
     1981 gcggctgcgc tcgcagggct ttctctcgcg ttcgtcctgt tcggtggcgg attcaccggg
     2041 gccgcgactg aggctgggtc ggtagccgct gccgggcagg cagggaaatt cggtgcggct
     2101 ttgggtatcg gtctgctcgg gctcgccttc tgcgacgatc gtgcgccagc cccagcaatc
     2161 ccgcccgcgc cgatcgacga cggagacgac tcgggaggca cgtacgtcaa gcccgttccg
     2221 gagtgtccag tcgaagagtc ggagggcggg acgtgctagt agcgcgccaa cgtccttcga
     2281 actaggcgct cagcccagct tcgcgatgat gattttggcc atcgccacgt cgggcgcgta
     2341 gttgtcggcg aactcagcgt gcgtcagtcc tgagacttcg atgcacttgt tgccgagttt
     2401 gacatccagg ccgatctcgt ggccgaacgc ttcgtcgccg atgcccgtca ccgtgacgcc
     2461 ggggccgtcg gcttcacgca gctgggtgta acaccctgcc agcgagatca cctgaatcgt
     2521 caagtcttgg atgtcgatgg gatccgaaga gcctttgttt gcgtagtcgc aggcgtcgat
     2581 gcccggctcc acgtgctgcg gcgtggcggc tccgtaggtc acctcgttga tcgcggatgc
     2641 ttgagccgac gtcatgagcg aacagacgtc gatggctcca ccgccggctg cgggcgccgg
     2701 tacggtcgaa gccgccgagc ttgccgcggt gctggcactc gccggcgcgc tcgattcaac
     2761 cgactggctc gcaaccgacg cacgggggga cgccgtcggg gtggcctcgt ccgcagctgt
     2821 cgcggtcgcg ggcagcgccg tcgccggcgt cggcgtggtc ttggacttcg ttgtcggatt
     2881 gcacgcggct gacgacaacg cgacgacaac gatggctgtg gttgctgata gcgctcggac
     2941 ctgcatggct gctccctact cggcggctcg gcgactggct ctgccgccaa atcgaagcta
     3001 ggtgcgcagc gtgaccgtgg aatcggggca aaccctcgat cagggcggga cgccgatggg
     3061 cgggcgtacg ctggccgcgc gcccgctcca cctgccacgc cgccatgggg gaaaccgatg
     3121 atcgagtcgg accaacttcg tggggctgat cccgcggacc tgatcggacg cgctgcggag
     3181 cggctcgcga tcgtgtctgc gctcgacgaa gcggccgacc gcaccgccgc cgttctccta
     3241 acaggcgatg cgggcatcgg caagacagcg atctgggagt cgatcgtcga gcagcgacgc
     3301 gcggctggcg atcacgtgtt gatctcgcgc gcaacgtcgg ccgaagcccg gctgccgtgg
     3361 gtcggcatga cggacctcct gcgcgcgatg ccgtcgacga cactgcaatc cctgccggat
     3421 gtgcaacgcc gtgcgctcga ggtcgtgtca ctccagtccg gatcagtgca cggtggtagc
     3481 tcggagacac tcgacgaacg catggtaggc accgcactgc tgtcggcgtt gcagtccgcg
     3541 acgaacaccg cacccgtgct gttggctatc gacgacctgc cctacctcga caccgcgagc
     3601 gcatcggcag tgacgtttgc cctacgacga atggaaggac cgcatcccgc gcggctgctc
     3661 gcgaccgttc gcgaccacga cgtcccactg ccggtgctgc agggtctgcc gaccgatcgg
     3721 tgctcggtga tctcgatcgg cccgctgacg cttggtgcac tgttcgatgt gctgcagagc
     3781 cgccgcggta tccggctcgc acggccgatg ttgctccgcg tctacgagac gtccggcggc
     3841 aacccgctct acgccttaga gcttgcccgc gctctcgacc gcctcgagat cagtccgaag
     3901 gcaggcacgc cgctgcccgt accggccgga ctcaacgcgt tggtcgatgc gcgcgtgcgt
     3961 gatctcccgg ccgaggtggc tgacgtcgtc gccgccacct cggccgcgtg gcgattcacc
     4021 gcgaccgatc gcgacgccga cgcgatcgag ctggccgtcg cggccgcgat ggtgatcgtc
     4081 gaggagccag cggtggtcgg cggcgcgcgg gtgatccgcg cagcgcaccc gctcttgagc
     4141 gcggccgcgt acaacggcct aacgacgtcc cgtcgccgac agctgcacgc gcggctcgct
     4201 gccgcagccg atgaccctgt cgagcgggtg cggcatgctg cgctcgcggc gacccacccg
     4261 caggccaccc tcgccgccga tctcgatgtc ggcgtcgccg cggcgctcgc cgccggcgtc
     4321 ccggatattg ccgttggtct tgcgcagttg tcgctcgaac acacgaccga cgttgccctg
     4381 cgtcccgcgc gcctcgatcg cctcgccgat gcgcagctac gttccgggga cagctcgggc
     4441 gcgtggcagt cgcaatcgga ggcgatcgcg gcgaccgagg cgggggcgga gcgtgcccgc
     4501 cgtcgcatca gactcgccga gattgccgtt gaagtaacga gctggccgga cgccgagcgg
     4561 gagctgcagg tggcgatcga tgaggccgca ggcgacccgc tcgtgttggc cgaagcgttg
     4621 cttaccctcg cggccgtcac ggacgacacc gacgtggcgg tgacctccgc gcagcgcgcc
     4681 gtcgatctcc tcgacgcgca gggcgatccg gatccgatga tcttgtctgg tgcgctcggt
     4741 caactcgcag gcgcgagctt tcggtccggc tatgggctcg atcgcgagtt gtttgcccgg
     4801 gccatcgaca tcgagcgtgg acatccctcg cggcgactct ccgatcgggc ggacgccgcc
     4861 tatgcggcgt tgctgaagta cgccgacgac attgacgcag cagaggaccg gctgctcgca
     4921 ctgctcgaag agacgcgtgc aactggtgat ctttcgtcca tcacgtacgc gctcggtcac
     4981 cttgtgccga tctacctgtg gcgtgggcag cttgcgcgtg gccggggcta cgcggacgag
     5041 cacctcgagg tttccatcca aggcgagcta caaggccaag agacctcagc gcgctacaac
     5101 ctcggccttg ccatggcgta ccagggtcag ctcgatgagg cctcacaagt cctgctcggg
     5161 ttgcttgccg ccgagagcac cgactcctgg attgttcatc gcgtccacgc tgtcctcggc
     5221 ttcgtcgctt tgtcccgaaa cgacgcgacc ggggcggttg agcacaccga tctgtggcat
     5281 gacgcgttgg tggaaatgca ctttggtgaa cctggctaca gtcgcgggca cctcgatcac
     5341 ctgtgcgcac tcatcggtgc cggtcggacg gcggatgccg ccacgttttg tgatgagctc
     5401 tccgagcagg cacaacggtc gggtcgcgag tcggccgccg cggtcgtatc gaccggacgc
     5461 gcaatgctcg aggcgcatgc aggacggttg agcaatgcgc tcgcactttc gacgtccgcg
     5521 ctgtcttggt acgactcctc gccgctgcgc ttcgatcgcg cacgcacgtt gctgatcgcc
     5581 ggccggatca gccgtcgcgc gaaggccaag tcagacgcgc gagctctgct caccgaggct
     5641 gagcacgagt tcgcctcctt cggcgcaact gcgtggcagg aacaggcggc ggctgagctc
     5701 gcgcgagtca acgtgcgtcc gtctgcgccg gctgaactga cggagaccga gcggctggtc
     5761 gcccagctcg ccgcatcggg actgagcaac aaggaagtcg ccgaccgcac gttccttgcg
     5821 gtcaagaccg tcgaagccaa tctggcgcgc gcgtaccgca agctcgggat tcgttcgcgc
     5881 gcggagctcg gagctcggat gggagtggta ccgcagcgcg gcgctcagta ggcgtgcgga
     5941 tggcgctcgg cggacaccaa ccggtcgcac cgcaagtcag cgcgccgagc cagctcaagg
     6001 accccggcgt catcgaccgc ccggaactcg aagaacacca cctcgtcggc cgggatgagg
     6061 gtgctcgcga caaatgtcgc gtcggcgccg cccaactccg tgagtcgccg acagacggca
     6121 tcgacgtccg cggcggtcac ccccggccaa tagcgctcta cgagaaatcc ggccatgtgc
     6181 agaccgtagg tccgcccttc gaccggcgcc agatgtgtcg ccctgatcga gggtttgccc
     6241 cgattcccct atcgagccgc gaatctagcg tcggcaacat gaacacaaac ctcaccgcga
     6301 ccccgcgtcg atggtcgagt gcggcctcgt catgtagttc ccgtcagtcg tcggcgacct
     6361 gcggggccac cttcgtgccg agcagttcga tgccgcgcag caggtcggcg tgggcgagcc
     6421 gcggattcgt catctgcatc gagacccggt caacaccgcc caactgcgcc gatatgcgcc
     6481 gcagcttggt cgcgacagtg tcagggtcgc caagaaagaa cgcgccgtca ggaccactcg
     6541 tggcgtcgaa ctgtgcgcgc gtcggcggcg cgaacccgcg ctcctgtgac gccctggtga
     6601 acatttggtg ccagcccgga tagatggtgt cggaggcaga ccggacgctg tcggcgacga
     6661 agccgaacac gtgcagaccc acctgtaggt gttcggggtc gtgacccgcc tcggcgcccg
     6721 ctcgtcggta cagatctacc agcggggcaa atcgccgcgg ttcgccgccg atgatggcca
     6781 ccatcagcgg cagcccaagc aggcccgcac gcacgaacga ctcgggcgtg cctccgaccc
     6841 ccacccagat cggcagcggg ttctgcaccg gacgtggata cacgccttgc ccggtgaggg
     6901 cggcgcggtg tcgaccggac cacgtgacgt ggacgtcgtc gcggatcttc aacaggaggt
     6961 caagcttctc ggcaaaaagc gtgtcgtagt cgtcgaggtc cagtccgaac aaggggaacg
     7021 cctcgatgaa cgagccgcgc ccgacgatga ggtcgatgcg gcccttcgaa atgagatcga
     7081 gggtggcgaa ttgctggaac acccgcaccg ggtcggccgc gctgagcacc gtcacggcac
     7141 tgcccagccg gatccgagag gtccgcgcag cggccgcgcc gaggatcacc ggtggggcgg
     7201 aatcatagaa ctccacccgg tggtgctcgc caatcccaaa ggagtacacg cctgcggcat
     7261 ccgcgagcgc gatttcctcc agcaggtgtt cgattcgctc ctcgggaccg acctcgcgtc
     7321 cggtcaccgg atcggtcacc gccgagacga agctgtcgac gccgacgtgc atcgtggcct
     7381 cctgtccgtc agtcgaggtc gaatgcggcg tccaagggtg ggcgccgacc ctgaggcagc
     7441 cgagatgtag ctgaggcagg tgtctgcacc tgcctcagca cccctaaggc tgcctgacgt
     7501 gatcaacttc cgagaaggcc ggggcgactt ctggttggcg gtggaggtgg gatttgaact
     7561 cccaaaatcg ggtgttttcg cgtggcccct c
//
LOCUS       DATCBJ010000079         4717 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_101638, whole genome shotgun sequence.
ACCESSION   DATCBJ010000079 DATCBJ010000000
VERSION     DATCBJ010000079.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 4717)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4717)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4717
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_101638"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..504)
                     /locus_tag="VII50_01710"
     CDS             complement(<1..504)
                     /locus_tag="VII50_01710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY5049587.1"
                     /translation="MVASGPERGTILVVDDDADIVRFVEMNLRLEGYRVLTARDGLEA
                     LEMVAAEAPDLVLLDVMLPGVDGVEVTRRLRADSRTSTLPVIMLTAKTMTADRVLGLT
                     TGADDYIIKPFDTLELVARVRSTLRRNAEARAVSPLTSLPGNVRIEDEIASRVSSGSP
                     FAVAYLDL"
     gene            complement(556..1233)
                     /gene="rpe"
                     /locus_tag="VII50_01715"
     CDS             complement(556..1233)
                     /gene="rpe"
                     /locus_tag="VII50_01715"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HEY5049588.1"
                     /translation="MSVQIAPSILSADFARLADEAAAVATVAEWLHVDVMDNHFVPNL
                     TLGAPVVAALHAATTLPLDCHLMIDDPDRWAATYAEVGAGNVTFHAEAAADVVQTARS
                     IRAAGARAGLAVKPGTDVENYLEVLGEFDMLLVMTVEPGFGGQEFLAAMMPKVARARQ
                     YLRDTGLPLWIQVDGGVTEETIGECARAGADVFVAGSAVYGAHDPAAAVTALRHRASV
                     FAAEAPA"
     gene            complement(1288..2685)
                     /locus_tag="VII50_01720"
     CDS             complement(1288..2685)
                     /locus_tag="VII50_01720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HEY5049589.1"
                     /translation="MKATPPAAARPPRVPRVPADPVRVVALDALRTVREQDAYANLVL
                     PGLLASRRLEARDAAFATELVYGTLRALGTYDAILGDLVDRPLDALDPLVLDVLRLGT
                     HQLLATRVGDHAAVDESVKLVRDKVGHGPTGLVNAVLRKVAARDLAGWCTAISPRFDV
                     DPIGHLSLTQSHPRWIVEGFRDALDGDLDETMALLAADNVRPSVTLVARPGRCDVEEL
                     VALGARLGRWAPTAAILDGGAPTALAAVREGRAGVQDEGSQLVALALAAAPIVGSDER
                     WLDMCAGPGGKAALLAGLASGRGARLLAVERQPHRARLVSHAVADTADVAVADGTAPP
                     WVPASFDRVLVDVPCSGLGALRRRPEARWRRTLADVEALRPQQVALLRSALDAVRPGG
                     LVAYVTCSPHLRETRSVVDEAISGRPGAGVERIDVRPYLPGVDLLGDGPDVQLWPHRH
                     GTDAMYIALLRRGLV"
     gene            complement(2682..3623)
                     /gene="fmt"
                     /locus_tag="VII50_01725"
     CDS             complement(2682..3623)
                     /gene="fmt"
                     /locus_tag="VII50_01725"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HEY5049590.1"
                     /translation="MRILFAGTPAVALPALETLLASPHELLAVITRPDAAAGRGRVAT
                     GSPVGSRAQEAGIEVLKPTKPADPQFQQRLRALAPDCVAVVAYGALVPPSALEIPRLG
                     WVNLHFSLLPAWRGAAPVQHAILHGDDITGATTFELEAGLDTGPVLGVLTEEIRPRDT
                     AGELLGRLSIAGAGLLAATLDGLAAGTLEAVPQRLDGVSLAPKITPADAHLDWSAPAM
                     HVDRLIRACTPAPGPWTTFRGRRLKLGPVLPALTGISTQLAAGAVAVVRDGVEAVLVG
                     TATTPVMLTTVQPEGKAPMAAVAWARGARLLPDDRFS"
     gene            complement(3678..4220)
                     /gene="def"
                     /locus_tag="VII50_01730"
     CDS             complement(3678..4220)
                     /gene="def"
                     /locus_tag="VII50_01730"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HEY5049591.1"
                     /translation="MAVQPIRLFGDPVLRTPAEPVTTFDKELRNLVRDLIDTMRDAPG
                     VGLAAPQIGVGLRVFSYHVDDEVGHLINPSLDLSDEQQDGDEGCLSFPGLVYPCKRSL
                     FAVAKGFNEFGEPIVIEGTDLLARCVQHETDHLDGVLFIDRLELEQRKFAMRAVREAD
                     WAGDPVRVKVSPHATHGMAL"
     gene            4452..>4717
                     /locus_tag="VII50_01735"
     CDS             4452..>4717
                     /locus_tag="VII50_01735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5049592.1"
                     /translation="MGEDQLGPWWVTPEPGAVPPQDGPGMVPPHLQSPPPWPGNSVLP
                     ASPESARRSLSAAAIAAVLAGLLIVVGGGGYLVLHHPKSSSPGV"
BASE COUNT          790 a         1639 c         1614 g          674 t
ORIGIN      
        1 gaggtcgagg taggcgacgg cgaacgggct gccactactt actcgactgg cgatctcgtc
       61 ctcgatcctg acgtttcccg gcaggctggt cagtggtgaa acggcacgcg cttctgcgtt
      121 gcgacgcaac gtcgatcgaa cccgggcaac aagctcgagc gtgtcgaacg gcttgatgat
      181 gtagtcgtcg gcgccggtcg tcagaccgag cacacggtcg gcggtcatcg tcttggcggt
      241 aagcatgatg acgggcagcg tcgacgttcg gctgtccgcg cgcaaccgcc gggtcacctc
      301 aaccccgtcg acgccaggaa gcatgacgtc gagcaacacg aggtcgggtg cctcggccgc
      361 caccatttcc agcgcctcga gaccgtcgcg agcggtgagc actcgatagc cttcgagacg
      421 caggttcatc tcgacgaagc ggacgatgtc cgcgtcgtcg tcgacgacca ggatcgtgcc
      481 tcgctcagga cctgacgcga ccactccact gcctctcaac gcacttcaga acctgtctca
      541 ccagggtcat cggccttatg ccggcgcttc tgcagcgaag acgctggccc gatggcgcaa
      601 ggcagtcact gcggcggccg ggtcgtgcgc gccgtaaacc gcggatccgg ccacgaacac
      661 gtcggcaccg gcccgcgcgc actccccgat cgtctcctcc gttacgccgc cgtcgacctg
      721 aatccacagc ggtaggccgg tgtcgcggag gtactgtcga gcgcgggcca ccttgggcat
      781 catcgcggcg aggaactcct gcccgccaaa accgggctcg acggtcatca ccagcagcat
      841 gtcgaactcg ccgagcacct cgagatagtt ctcgacatcc gtgcccggct tcacggcgag
      901 accagctcgc gcgcccgcgg ccctgatcga gcgcgccgtc tggaccacgt cggcggccgc
      961 ctcggcgtga aacgtcacgt tgcctgcgcc aacctcggcg taggtcgccg cccagcggtc
     1021 gggatcgtcg atcatcaggt ggcagtcgag cggcagggtt gtcgcggcgt gcagagcagc
     1081 gacgaccgga gcgcccagag tcaggttcgg cacgaagtgg ttgtccatga cgtcgacgtg
     1141 caaccactcg gcgaccgtgg caaccgcagc cgcctcgtcg gccagcctgg cgaaatccgc
     1201 agacaggatg ctcggcgcaa tctgcaccga catggccgcg ccgccttcca gataccagag
     1261 aagtcccctg agtctatggc gagggcgtca caccaagccg cgacgcaaca gcgcgatata
     1321 catcgcgtcg gtgccgtgcc gatgcggcca caactgcacg tcgggaccat cgccgagcag
     1381 gtcgacaccg ggcaggtagg gccggacgtc gatgcgctcg acacccgcgc cgggtcgccc
     1441 gctaatcgcc tcgtcgacga ccgacctggt ctcgcgcaga tgaggcgagc aggtgacgta
     1501 ggcgacgagg ccaccaggcc ggacggcgtc gagcgcagac cgcaacagtg ccacctgctg
     1561 gggccgcagg gcttcgacgt ccgcgagcgt gcgccgccaa cgagcctcgg ggcggcgccg
     1621 caacgcaccg agcccagaac aggggacgtc gaccagaacg cgatcaaacg atgcgggcac
     1681 ccagggcggc gcagtgccgt cggcgaccgc aacgtccgcg gtgtcggcga ccgcgtggct
     1741 gaccaaccgc gcccgatgag gttggcgttc gacggctagc agcctcgcgc cgcgcccgga
     1801 ggccaggccg gccagcagtg ccgccttgcc acccggtcca gcgcacatat cgagccaccg
     1861 ctcgtcgctg cccacgatcg gggccgccgc aagcgcgagc gcgaccagtt ggctgccttc
     1921 gtcctgcacg ccggcgcggc cctcgcgaac ggcggcaagc gcggtcgggg cccccccgtc
     1981 gaggatcgcc gccgtcggcg cccaccgacc cagtcgtgct ccgagcgcta cgagctcctc
     2041 gacatcgcag cggccgggcc gggcgacgag ggtgaccgat ggacggacgt tgtcggcggc
     2101 aagcagcgcc atcgtttcgt ccaggtcacc gtcaagggcg tcccggaatc cttcgacgat
     2161 ccagcgcgga tggctttggg tcaacgagag gtgcccgatc gggtcgacgt cgaagcgcgg
     2221 cgagatggcg gtgcaccagc cggccaagtc gcgagcagcc accttgcgca agaccgcgtt
     2281 gaccagaccg gtcggcccgt gcccgacctt gtcgcgcacc agcttcacgg attcgtcgac
     2341 cgcagcgtga tcgcccaccc gagtcgccag cagctgatgg gtgcctaggc gcaggacgtc
     2401 gaggacaagc ggatcgaggg cgtcgagggg gcgatcgacg aggtcgccaa ggatcgcgtc
     2461 gtacgtgccc aacgcccgca atgtgccgta cacgagttcg gtcgcgaacg ccgcatcgcg
     2521 agcctcgagc cggcgactgg cgagcagccc aggcagcacc agattggcgt aggcgtcctg
     2581 ctcgcgcacg gtgcgtagcg cgtccaaggc gacgacgcgc accgggtcgg caggtacccg
     2641 cggcacccgc ggcggccgtg ccgcggcggg tggggtggcc ttcacgagaa ccggtcgtcg
     2701 ggcagcagtc gggcgccgcg cgcccacgcg acggcagcca tcggggcctt gccctcgggc
     2761 tggaccgtcg tcaacatgac cggcgtcgtc gcggttccga cgagcaccgc ctcgaccccg
     2821 tcgcgcacga ccgccacggc cccagcagct agctgcgtgc tgatgcccgt gagcgcgggc
     2881 agcaccgggc ctagcttgag tcgccgcccg cgaaacgtcg tccacggtcc tggcgccggc
     2941 gtgcaggcgc ggatcaagcg gtcgacgtgc atcgccggcg cggaccagtc gaggtgggca
     3001 tcggccggcg taatcttggg cgccaagctg acgccgtcca ggcgttgggg taccgcctcc
     3061 agcgttcccg cggcgagccc atccagtgtc gccgcgagca ggcctgcacc cgcaatcgac
     3121 agccgaccga gcagctcgcc cgcggtatcg cggggccgga tttcctcggt cagcacgccc
     3181 aggaccgggc ccgtgtcgag cccggcttcg agctcgaacg tcgtcgcgcc cgtgatgtcg
     3241 tcaccatgca ggatcgcgtg ctggaccggc gccgcgccgc gccatgccgg cagcagggaa
     3301 aagtgcagat tgacccagcc gaggcgagga atttctagcg cggacggcgg gacaagcgcg
     3361 ccgtaggcga caaccgcaac gcaatcgggg gcgagcgcgc gcagccgctg ttggaactgc
     3421 ggatcggccg gcttggtcgg cttcaacacc tcgatgcctg cctcctgtgc ccgactgccg
     3481 acggggctgc cggtggcgac cctgccgcgc cccgccgcgg cgtccggccg ggtgatgacg
     3541 gcgagcagtt cgtgaggcga cgccaacagc gtctcgaggg ccggcaacgc aaccgccggg
     3601 gttccggcga acaggatgcg catggcgtca ggtcgtcacg gcgatagcga tgccaccggg
     3661 acggcgggtc acgctcgcta cagcgccatg ccgtgcgtcg cgtgcggcga gaccttcacc
     3721 cgcaccgggt caccagccca gtccgcctcg cgaacggccc gcatcgcgaa cttgcgctgc
     3781 tcgagctcga gccggtcgat aaacaagacc ccgtcaaggt ggtcggtttc atgctgaacg
     3841 cagcgcgcga gcaggtcggt accttcgatg acgatcggtt cgccaaactc gttgaacccc
     3901 ttggctaccg caaacagcga ccgcttgcac gggtagacga gtccggggaa cgagaggcag
     3961 ccctcgtcgc cgtcctgctg ctcgtcggag aggtcgagcg acgggttgat cagatgcccg
     4021 acttcgtcgt caacgtggta gctgaagaca cgaagaccga cgccgatctg cggcgccgcg
     4081 aggccgacgc ccggagcatc gcgcatggtg tcgatgaggt cgcgcaccag gttgcgcaac
     4141 tccttgtcga aggtcgtgac cggttcggcc ggcgtacgca agacaggatc gccgaagagg
     4201 cgtatcggct gaacggccac gcgaactcct gacgactctt ggcacgggcg atccacaccc
     4261 ggccgggtgc acgcttgcgt cgattctacc gatgcaccga cgcagggtca gagttgcgcg
     4321 tcagcgtaca gaactagtcg aatccgccta atcggtgtca tgacgacaga ttggccttca
     4381 gtcccctcaa gaagcggtcg aggccaaggc gaagccgtgc gtctcgggca gtgcctatcg
     4441 atggagtacg catgggtgag gatcagctcg gaccgtggtg ggtcactcct gagccgggag
     4501 cagtgccgcc gcaggacggc ccaggaatgg tgccgccgca tcttcagtcg ccaccgccgt
     4561 ggccgggcaa cagtgtgctg ccggcgagcc ccgaatcggc acgccgctca ttgtcggctg
     4621 ccgcgatagc tgccgtgctg gccggcttgc tcatcgttgt cggtggcggc ggctacttgg
     4681 tgttgcacca cccgaaatcc tcgtcgcccg gcgtcca
//
LOCUS       DATCBJ010000080         2419 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_65299, whole genome shotgun sequence.
ACCESSION   DATCBJ010000080 DATCBJ010000000
VERSION     DATCBJ010000080.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2419)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2419)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2419
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_65299"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..646
                     /locus_tag="VII50_01740"
     CDS             <1..646
                     /locus_tag="VII50_01740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="carbamoyl phosphate synthase small subunit"
                     /protein_id="HEY5049593.1"
                     /translation="DLAGQVSTREPYVVPAIGPRRFTVAAIDLGIKAMTPHRMAQRGM
                     EVHVLPAVSSIDDVAAINPDGVFFSNGPGDPAASVGPVQLLAGVLARRLPFFGICFGN
                     QLLGRALGFGTYKLGYGHRGINLPVIDRATGKVEVTAHNHGFAVDAPLTGETATPFGR
                     ARVSHVCLNDDVVEGLECLDTPAFSVQYHPEAAAGPHDAAYLFDRFGQLMSAGR"
     gene            646..>2419
                     /gene="carB"
                     /locus_tag="VII50_01745"
     CDS             646..>2419
                     /gene="carB"
                     /locus_tag="VII50_01745"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HEY5049594.1"
                     /translation="MPKREDLKSVLVIGSGPIVIGQACEFDYSGTQACRVLKAEGLRV
                     SLVNSNPATIMTDPEFADATYVEPITPEFVEMIIAKERPDALLATLGGQTALNTAVAL
                     HEAGVLEKYNVELIGANIEAIQAGENRQRFKEIVASVGAESARSVICHTVADCLGAVD
                     ELRYPVVVRPSFTMGGAGSGIAYDEVDLRRIAGAGLQASPTTEVLLEESILGWKEYEL
                     ELMRDRNDNVVVVCSIENLDPMGVHTGDSITVAPALTLTDREYQHLRDIAIAVLRAVG
                     VDTGGCNIQFAVQPETGRVIVVEMNPRVSRSSALASKATGFPIAKIAAKLALGYTLDE
                     IPNDITRKTPASFEPSIDYVVVKVPRFAFEKFPGADDTLTTTMKSVGEAMSIGRNFTE
                     ALQKALRSLEQTGSSFRWDGEIGDRRELIEQARRPTDGRIVTVQQAIRAGATVDELHE
                     ATGIDRWFLDQILLLDEVAQTVAQAESLDLATLHRAKRHGFCDAQIARIRGLTEATVR
                     GVRHDLNLRPVYKTVDTCAAEFAASTPYHYSSYDDETEVVPRTKPAVLILGSGPNRIG
                     QGVEFDYSCVHAAWALQAAGRAAVV"
BASE COUNT          422 a          793 c          791 g          413 t
ORIGIN      
        1 cgatttggcc ggccaggtgt cgacacgcga gccgtacgtg gtgcccgcga tcggtccgcg
       61 gcgtttcacg gtcgccgcga tcgacctcgg tatcaaggcg atgacgccgc atcggatggc
      121 gcaacgcggc atggaggtgc acgtgctgcc ggccgtctcg agcatcgacg acgtcgcggc
      181 gatcaatccc gatggggtct tcttctccaa cggcccaggg gatccggctg catcggtcgg
      241 ccccgtgcag ctgctcgccg gcgttcttgc tcgccggttg ccgttcttcg ggatctgttt
      301 cggcaatcag ttacttggcc gtgccctcgg gttcggcacc tacaagctgg gttacgggca
      361 ccggggcatc aacctgccgg tcatcgaccg cgccaccggc aaggtcgaag tgaccgccca
      421 caatcacggc tttgctgtcg acgctccgct gacgggtgag acggcgacgc cgttcggtcg
      481 cgcgcgcgtc agccatgtgt gtctaaacga tgacgtcgtc gagggtctgg agtgtcttga
      541 tacgccggcg ttctcggtgc agtaccaccc tgaggccgcc gcaggtccac acgatgctgc
      601 gtacctgttc gaccgcttcg gccagctcat gagtgcgggc cgctgatgcc gaagcgggaa
      661 gacctgaaat cggtactggt catcggctcc gggccgatcg tgatcggaca ggcctgcgag
      721 ttcgactact cgggtaccca ggcctgccga gtgctgaagg cagaaggcct acgggtctcg
      781 ctggtgaact ccaatccggc cacgatcatg accgatcccg aattcgctga cgcgacctac
      841 gtcgagccga tcacgcccga gttcgtcgag atgatcatcg ccaaggaacg gcccgatgcg
      901 ctgctcgcga cgctcggcgg tcagacggcg ctgaacaccg cggtggcgtt gcacgaggcg
      961 ggcgtgctgg agaaatacaa cgtcgaactc atcggtgcga acatcgaggc gatccaggcg
     1021 ggtgagaacc ggcagcggtt caaggaaatc gtcgcatcgg tcggtgccga atcggcccgc
     1081 tcggtgatct gccacaccgt tgccgactgc ttaggtgcgg tcgacgagct tcggtatccg
     1141 gtcgtcgtcc ggccctcgtt cacgatgggt ggcgccggct ccggaatcgc ctacgacgag
     1201 gtcgacctga ggcggatcgc cggcgccggc ctgcaggcca gcccgaccac cgaggtcttg
     1261 ctcgaggagt cgatcctcgg ctggaaagag tacgagctcg aactgatgcg cgaccgcaac
     1321 gacaacgtcg tcgtcgtgtg ctcgatcgag aaccttgacc cgatgggtgt gcacactggc
     1381 gactcaatta cggtcgcgcc tgcactgacc ttgaccgatc gcgaatacca gcacctgcgt
     1441 gatatcgcga tcgcggtgct gcgtgcggtc ggcgtcgaca caggcggctg caacatccag
     1501 ttcgctgtgc agccggagac gggccgggtc atcgtcgtgg agatgaaccc gcgggtgtcc
     1561 cggtcatcgg cgttggcgag caaggccacc ggattcccga tcgccaagat cgccgcaaaa
     1621 ctggcgctcg gctacaccct cgacgagatc cccaacgaca tcaccaggaa gacgccagcc
     1681 agtttcgagc cgagcatcga ctacgtggtc gtgaaggtgc cgcggttcgc gttcgagaag
     1741 ttccccggcg ctgacgacac gttgacgacg acgatgaaga gcgtcggcga agcgatgtcg
     1801 atcggccgca acttcactga ggccttgcag aaggcgctgc gatcgttgga gcagaccggc
     1861 tcgtcatttc gatgggacgg cgagatcggc gaccgccgtg agctgatcga gcaggctcgg
     1921 cgtccgaccg acggccgcat cgtcaccgtg cagcaggcga tccgcgccgg cgcaaccgtc
     1981 gacgaactgc acgaggcgac cggcatcgac cggtggttcc tcgatcagat cctgctgctc
     2041 gacgaggtcg cgcaaactgt cgcgcaggcg gagtcacttg accttgcaac acttcatcgg
     2101 gccaagcggc acggcttctg cgatgcgcag atcgcgcgca tccggggact gaccgaggcc
     2161 acggtgcgtg gcgtgcggca cgacctcaac ctgcgacccg tctacaagac cgtcgatacc
     2221 tgcgccgctg agttcgccgc cagcaccccg taccactact cgagctacga cgacgagacc
     2281 gaggtcgtgc cacgcaccaa acccgccgtc ctcatcctcg gatcgggccc gaaccgcatc
     2341 ggccagggtg tcgagttcga ctactcgtgc gtccacgccg cgtgggccct gcaagccgcc
     2401 ggccgcgccg ccgtcgtga
//
LOCUS       DATCBJ010000081         2964 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_101405, whole genome shotgun sequence.
ACCESSION   DATCBJ010000081 DATCBJ010000000
VERSION     DATCBJ010000081.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2964)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2964)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2964
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_101405"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..402
                     /locus_tag="VII50_01750"
     CDS             <1..402
                     /locus_tag="VII50_01750"
                     /inference="COORDINATES: protein motif:HMM:NF018749.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1349 domain-containing protein"
                     /protein_id="HEY5049595.1"
                     /translation="EVEFGATFDAGVLLVWANERSWAKLCLEYSPAGQPMVVSVVTRG
                     RSDDANSRDVEGLGTWLRISRSTAGSYAFHAATDGVHWSLIRHFHLDTGADDAVRIGF
                     LAQSPTGEGCRASFSQLRYLPEALDDLRGGD"
     gene            complement(417..1376)
                     /locus_tag="VII50_01755"
     CDS             complement(417..1376)
                     /locus_tag="VII50_01755"
                     /inference="COORDINATES: protein motif:HMM:NF012607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase family protein"
                     /protein_id="HEY5049596.1"
                     /translation="MTHRVLVLDDYQDVARKFGPWQQLSGVAGSPFDLDVRTEHLDGE
                     ALTAALQGVDIVVAMRERTPFTRDVLAQAPALKLLVTTGMANASIDLDACRELGVEVR
                     GTTGLRASTAELTWGLILTLARQICAEDRQVRGGGWQHTLGFELAGARLGVVGLGKLG
                     SEVAKIGLAFSMDVVAWSQNLQPEYASELGVRAVAKHELFATSDVVTLHLKLSDRTRG
                     VVGADELAAMSRTAYLVNTSRGPLIDQDALIDALRTDSIAGAGLDVFDIEPLPADHPL
                     RTLPNTVLTPHVGYVTTATYERWWPQVVEDIRSWADGEAVRVL"
     gene            1436..2101
                     /locus_tag="VII50_01760"
     CDS             1436..2101
                     /locus_tag="VII50_01760"
                     /inference="COORDINATES: protein motif:HMM:NF013936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HEY5049597.1"
                     /translation="MDADSAADRPLIQQMVVIHRVFRREFGLLPALIRNVAPDDLERA
                     KVVADHATGLLRFVHVHHSGEDEFLWPVLLERVEIETDLIRRMEDQHLHVAALIPRAQ
                     EQLPGWAAQPSAERGDELATTFEDIASVLDEHLGEEEKQILPLVETHLTVAEWAQLGQ
                     HATASMAPPELMASLAAILEEADDDERAMFTVVLPPPALTIFVEQAEPAYREQMRRLR
                     AGV"
     gene            2104..>2964
                     /locus_tag="VII50_01765"
     CDS             2104..>2964
                     /locus_tag="VII50_01765"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF013345.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HEY5049598.1"
                     /translation="MPVAGFDDEVLDWRWKGFPALAAGQRVSQFIASRPLLFEAGFSW
                     PIATLSTSAIDNNVAVLAAWCEDNDVLLAPHGKTTMAPALFARQLAAGAWAMTAATPW
                     QARVYRAHGVRRVLLANELVDVDFMRWLVAERASDPDFEFICYVDSVAGVDLLGHALP
                     AGDATPAQPLGVLVEIGPMGGRTGCRTRDAVRAVAHAVQQHASLALIGVAGFEGPFGH
                     GRDASTLTGIRTFVEELADTLRELDTDALLDPRSPYFVLTCGGSEQVDVVTSVLREAP
                     ECSRPVRSVLR"
BASE COUNT          478 a         1001 c          995 g          490 t
ORIGIN      
        1 gaggtcgagt tcggcgccac cttcgacgcc ggcgtgcttc tcgtgtgggc gaacgagcga
       61 tcgtgggcca aactctgcct cgaatactcg ccggcggggc agccaatggt cgtctccgtc
      121 gtcaccaggg ggcgatccga cgacgccaac agcagggacg tcgaggggct ggggacgtgg
      181 ctgcgaatct cgaggtcgac ggccggcagc tacgccttcc atgccgcgac cgatggcgtg
      241 cactggagcc tgatccgcca cttccatctc gacactgggg ccgacgacgc cgtgcgaatt
      301 ggcttcctcg cacaatcgcc gaccggcgaa gggtgccggg cgagcttctc ccagctgcgc
      361 tacctgcccg aggccctcga cgatctgcgc ggcggggact agaggcggcg aacgccctac
      421 agcacccgga cggcttcgcc gtccgcccag ctcctgatgt cctcgaccac ctgaggccac
      481 cacctctcgt acgtcgctgt ggtcacgtag cccacatgcg gagtcagcac cgtgttcggg
      541 agggttcgca gggggtggtc ggcgggcagc ggctcgatgt cgaacacatc gagtccggct
      601 cctgcgatgg agtcggtccg cagcgcgtcg atcagggcgt cctggtcgat cagcgggccg
      661 cgtgacgtgt tcactaggta cgcggttcgc gacatcgcgg cgagttcgtc ggcccccacg
      721 acgccacggg tgcgatcgct gagcttgagg tgcagcgtga caacgtcgga ggtggcaaac
      781 aactcgtgct tggcgaccgc ccgaacgccg agctcgctgg cgtactcagg ctgcaggttc
      841 tggctccagg caacgacgtc catgctgaat gcgaggccga tcttggcgac ctcgctgccg
      901 agcttgccca agccgaccac tccgagccgc gcgcccgcga gttcgaagcc gagagtgtgt
      961 tgccaaccgc cgccgcgaac ctgacggtcc tccgcacaga tctggcgcgc gagcgtgaga
     1021 atcaaacccc aggtcaactc cgcggtcgac gcccgcagcc cggtggttcc tctcacctcg
     1081 acgccgagct cgcgacacgc gtcgaggtcg atggatgcgt tcgccatccc agtcgtgacg
     1141 agcagcttta gggcgggcgc ctgcgcgagc acgtcgcgcg tgaacggcgt tcgctcgcgc
     1201 atggcgacca cgatgtcgac gccctgcagc gcggctgtca atgcctcgcc gtcaaggtgc
     1261 tcggttctca cgtcgaggtc gaacggggac cccgcaactc cggacagttg ctgccacggc
     1321 ccgaacttcc gggcgacatc ttgatagtcg tcgagaacca gaacacgatg cgtcatgcac
     1381 gcatcctggc atcatgacaa gacgccattc acctgacagg aaaggaccgc cgccgatgga
     1441 cgccgacagc gccgccgatc gtccactgat tcagcagatg gtggtgatcc accgcgtctt
     1501 ccgccgcgag ttcggcctgc tgccggctct gatccgcaac gtcgcccctg atgatctcga
     1561 acgcgcgaag gtcgtcgccg accacgcgac cggtctgctg cgattcgtgc acgtgcacca
     1621 cagtggcgag gacgagttcc tctggccagt gttgctcgag cgggtcgaga tcgagacaga
     1681 cctgatccgc cggatggaag atcagcatct gcacgtcgcg gcgctgatcc cgcgtgcgca
     1741 ggagcagctg ccgggctggg ccgcgcagcc atcggcggaa cgaggcgacg agctcgcgac
     1801 aacgttcgaa gacatcgcgt cggtcctcga cgagcacctg ggcgaggaag agaagcagat
     1861 tctgccgctc gtcgagaccc acctcaccgt cgcggagtgg gcacagttgg gccagcacgc
     1921 gaccgccagc atggcaccgc cagagctgat ggcgagcctt gcggcgattc ttgaagaggc
     1981 ggacgacgac gagcgggcga tgttcacggt ggtactaccg ccccccgctc tcacgatctt
     2041 cgtcgagcag gctgagccgg cttaccggga gcagatgcgc cgactccgcg ccggcgtgtg
     2101 acggtgccgg tcgccggttt tgacgatgag gtgctcgact ggcgatggaa gggctttccc
     2161 gccctggctg ccgggcagcg agtgtcgcag ttcatcgcca gccgcccgtt gctgttcgaa
     2221 gctggattca gttggcccat cgccacgctg agcacctcgg cgatcgacaa caacgtcgcc
     2281 gttctcgctg cgtggtgcga agacaacgat gttctgctcg cgccacacgg caagacgacg
     2341 atggcgcccg ccctgttcgc gcgccagctg gcagccggtg cgtgggccat gacggctgcc
     2401 acgccgtggc aagcgcgcgt gtaccgggcg cacggtgtcc gccgcgtgct gttggccaac
     2461 gaactcgtcg acgtcgactt catgcggtgg ctggtcgccg aacgcgcctc ggatccagac
     2521 ttcgagttca tctgctacgt cgactcagtc gcgggcgtcg acctgctcgg ccacgcgttg
     2581 cccgctggcg atgccacacc tgcgcagccc ctcggcgtcc tcgttgagat cggtcccatg
     2641 ggtgggcgga ccggctgccg aacccgcgac gcagtacgcg ccgtcgcgca tgcggtgcaa
     2701 cagcacgcgt cgctggcgct catcggggtt gccggcttcg aggggccttt cgggcacggc
     2761 cgcgacgcca gcacgttgac tggaattcgg acgttcgtgg aggagctcgc ggacacgctg
     2821 cgcgagctcg acacggacgc actgctcgat ccgcgttcgc cgtacttcgt cctcacctgc
     2881 gggggcagcg agcaagtcga cgtcgtgaca tcggtgctgc gagaagcgcc ggagtgttcg
     2941 cggcccgttc gcagcgtgct gcgc
//
LOCUS       DATCBJ010000082         2187 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_324514, whole genome shotgun sequence.
ACCESSION   DATCBJ010000082 DATCBJ010000000
VERSION     DATCBJ010000082.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2187)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2187)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2187
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_324514"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..412)
                     /locus_tag="VII50_01770"
     CDS             complement(<1..412)
                     /locus_tag="VII50_01770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5049599.1"
                     /translation="MHQHVSLRDRLEALTEHREPVFEPSDDPTLVAFSKLFAESGLVA
                     FDASKRVRLDALSAIYDCETESRSGADTAPEVFVAIWTPVWSGEDPLTGFRTGQDSVA
                     FGSTPTVVMPHDERTAALLLLVTSTFVHLSASGAE"
     gene            complement(532..915)
                     /locus_tag="VII50_01775"
     CDS             complement(532..915)
                     /locus_tag="VII50_01775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5049600.1"
                     /translation="MSALVLDARAFVAVDRGDRAMVARLRAAERNGVELRTNGVVVAQ
                     VWRDPTGRQVELARLLRCVDVLAVDQYIGRDAGVLLAAAAAGDAVDATVVVMAFAGDR
                     ILTSGPGDIRALVAASGRAIVVVPC"
     gene            complement(912..1157)
                     /locus_tag="VII50_01780"
     CDS             complement(912..1157)
                     /locus_tag="VII50_01780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5049601.1"
                     /translation="MKVDKLSVSFDPDLGDAVRAAASRSGRGLSGWLADAAAARLRAE
                     ALGDFLDSWEAGHGPLTADELARACAELGLPGVDPAA"
     gene            1276..>2187
                     /locus_tag="VII50_01785"
     CDS             1276..>2187
                     /locus_tag="VII50_01785"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HEY5049602.1"
                     /translation="MDVGRGSLAINANAPDSAAPVQAETRWESRRFAAWSLRAGIVIA
                     PVIAAVIVMAVVSRVVPRPRSGSLLLLAGWWALVLASSLVALWATDRAARRLTPLAAL
                     LDLAVLFPGRAPTRFAIARKAGDLRALKTLSEAVLPGQQPGNVATAAEQILALVGSLR
                     AHDRHTRGHSERVRVYTDMIAAELRLPASAVDRLRWAALLHDIGKLRIPASVLNKPGK
                     LTSSEWAAIRRHPDAGAELAADLLPWLGPWGLAIAQHHEHFDGSGYPHGLSGTHISLA
                     GRIVAVADSFETMTGARPYKKARTRAAG"
BASE COUNT          361 a          775 c          711 g          340 t
ORIGIN      
        1 gttccgctcc actggcagac aggtgcacga aagtgctcgt caccaggagc agcagcgcag
       61 cggtccgctc gtcgtgcggc atgacgaccg ttggcgtgct gccgaatgcg accgagtctt
      121 gccccgtacg aaagccggtc aagggatcct cgccggacca gaccggcgtc cagatagcga
      181 cgaacacctc tggtgcggta tcggccccgg accgcgactc ggtttcgcag tcgtagatcg
      241 ccgagagcgc atcgagacga acgcgtttgc ttgcatcgaa cgcgactagc cccgactcgg
      301 cgaagagctt gctgaaggcg accagcgtcg gatcgtcgct cggttcgaac acgggctcgc
      361 ggtgttctgt caacgcctcc aggcgatcgc gcagtgacac gtgctgatgc atggcggctc
      421 cttcgtcgcc tccatggtca cacgctcggc gagactgtca ccgcgctgag cgaagcgatc
      481 ggcgccatcg agccacccaa gggcataccg gcgggcatgg tcgtgggcag gtcagcaggg
      541 cacgaccacg atcgctcggc ctgacgcagc gaccagagct cggatgtccc cgggaccact
      601 ggtgagtatc cggtctccgg cgaaagccat cacgacaaca gtggcgtcaa ccgcatcgcc
      661 cgcggcggca gcggccagga gcaccccggc gtcccgtccg atgtattggt cgacggccaa
      721 gacgtcgacg cagcgcagga gtctcgcaag ctccacctgc cgcccggtcg gatcccgcca
      781 gacttgcgcc acaacgaccc cgttggttcg cagttcgacg ccgttgcgtt cggctgcacg
      841 tagtcgggcg accatcgccc ggtctccccg atcgaccgcc acaaacgccc gggcatcgag
      901 cacgagagca ctcatgctgc gggatcgact cctggcaggc ccagttccgc gcacgcacgg
      961 gctagttcgt cggcggtgag cggcccatgg ccggcttccc agctgtcgag aaagtcgccc
     1021 agcgcctcgg cgcgcagtcg agccgcagcc gcgtcggcca gccaacctga aaggccccgc
     1081 ccgctgcgcg acgcggccgc gcgcaccgcg tcacccagat ctggatcgaa ggacacgctc
     1141 agcttgtcaa ccttcatgcg ttgagcatac tacggtacgt cctaccctgg tatgaaccgc
     1201 tcaatgtcca ggattcgggg cacagcggcc agcagatggc ccctcaacat tcgcgagcca
     1261 actgccgaca cctagatgga cgtcgggagg ggttcgttgg ctattaacgc gaacgctccc
     1321 gattcggctg cgccagtaca ggccgaaacc cgctgggaaa gccgccgttt tgcggcctgg
     1381 tcgttgcgcg cgggcatcgt gatcgcgccc gtcatcgccg cggtcattgt catggcggtc
     1441 gtcagccgcg tggtgcctcg accccgctcc ggctccctgc tgttgctcgc cggctggtgg
     1501 gcgctggtgc tcgccagctc gctggtcgcg ttgtgggcaa ccgaccgcgc cgcgcgccgg
     1561 ctgactccgc tcgccgcctt gctcgatctc gcggtgctgt ttcccggccg cgcgccgacc
     1621 cgattcgcga tcgccagaaa ggccggcgac ctgcgcgcac tcaagacact gtccgaggca
     1681 gtgcttccgg gccagcagcc gggcaatgtc gccaccgccg ccgaacagat cctggctctc
     1741 gtcggctcgc tgcgcgcaca cgatcggcac acgcgcggcc attccgagcg ggtgcgcgtc
     1801 tacaccgaca tgatcgccgc cgaactgcgg ctaccggcgt cggcggtcga ccgattgcga
     1861 tgggccgcgc tgctgcacga catcggcaag ctgcggatcc cggcgtcggt attgaacaag
     1921 cccgggaagc tcacaagctc ggagtgggca gcgattcgcc gacacccgga cgccggagcg
     1981 gagctcgccg cggatctgtt gccgtggctg ggtccgtggg gtctcgcgat cgcccagcat
     2041 cacgagcact tcgacggcag cggctacccg cacgggctga gcggaacgca tatctccctc
     2101 gccggccgaa tcgtcgccgt cgccgactcg ttcgagacga tgacaggagc gcgcccatac
     2161 aagaaggcgc gcacccgcgc ggccggg
//
LOCUS       DATCBJ010000083         2781 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_143312, whole genome shotgun sequence.
ACCESSION   DATCBJ010000083 DATCBJ010000000
VERSION     DATCBJ010000083.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2781)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2781)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2781
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_143312"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(360..653)
                     /locus_tag="VII50_01790"
     CDS             complement(360..653)
                     /locus_tag="VII50_01790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5049603.1"
                     /translation="MTGVTALVGTGVAMAATYPPAPPVTTQTAVPLITPAVAPVAVLG
                     EVVTKPAPTVATPSASLPFTGLDTMALVAVGAALVAGGTAMTVATRRRRSHSH"
     gene            complement(775..2685)
                     /locus_tag="VII50_01795"
     CDS             complement(775..2685)
                     /locus_tag="VII50_01795"
                     /inference="COORDINATES: protein motif:HMM:NF024594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4012 domain-containing protein"
                     /protein_id="HEY5049604.1"
                     /translation="MTGDRVDPEDPAAQADDSDEPDVPEESDATAQPDEWVAPIAVDE
                     VPRWRRIPPAAWAVFGVIVVLLGAVVWTGVKAWQLDRSISRSEVDVSYLRTDLGAGRF
                     TAFAPDTDRLQNDATAASSASHDPMWELAGHVPFVGRNLRAASVVAQSLSRIVNGALV
                     PLSRSHTFTVAASHNAGVSNLLTALGQDSTTIAAAVTTVEAANADVADLHTGGLLGFV
                     SRRVVSAQTKLGRATTQVQDLSALSTVAPALLGQDKPRTILLMFQTPAEERGTGGLIG
                     AWGELRTDKGAISLLAFGSLDSLAKVPVPPPGVSPDVVADYGSDVSLPQNFNLSASFP
                     DAAQIFSASWTTGPGRGVVPDAVVSIDPVALGHMLSATGPVKTSNGTELSATNASDIL
                     QKQEYYTFPGADQAPRIAFLGEVTRAVFARMTKPGYAVGALGRKIAQSAGTGDLEVWS
                     ADPTAEAKWVEMGVGGPLGSATAPGAATTVRLALNSLDGSKLGAYLTTSFTSTNVCSP
                     GPLLTITLRSTAPLDIPWYAGTHLSGLDITTMKLAYSLYLSPLWGVKSVSAAGQPAAV
                     SAQMEGGWRLIRGVIDVPRTATRIVAVQLTASAGASEITKILTEPEASPVATSVQGNV
                     TGGTACTAQPNG"
BASE COUNT          467 a          932 c          943 g          439 t
ORIGIN      
        1 ccgctagcgc gaccgcggtg ttgagcgcgg tcaccgacac gcgggtgtta ccgacggtgg
       61 cggcggcgcc gggatgcgcc ggactgcagg ccgcgacgga cagcgcaagc gcagctgtga
      121 ccgccatggc cgggacgacg gtagccgtgt gcgcgaacct gctgcgggac accgatgtcc
      181 ttccgtccgt tcacccgagt cggcgggtgg ctgggcttgc cgtacttgcc cgggggccat
      241 tgtcgcgggc gcaacggaca gccgtgaaac cagggtcctc cgggcatgca gcagggcgcc
      301 tggccgaggc cgggcgccct gctgtgtgaa gactagctgt gctcctacag aaggctagat
      361 cagtgcgagt ggctgcgacg cctgcgggtc gcgaccgtca tggccgtacc accagcgacg
      421 agcgcggcac cgacggcgac aagcgccatc gtgtcgagac cggtgaacgg caggctggcc
      481 gacggtgttg cgacggtcgg tgccggcttg gtcaccactt cgccgagcac tgcgacgggc
      541 gcaaccgcag gcgtaatcaa gggaaccgcg gtctgcgtgg tgacgggcgg cgcgggcgga
      601 taggtggccg ccatcgcaac gccggtcccc acgagtgcag tgacgcctgt catgcccccg
      661 acgacaagta gacgagctcc gtgcttcatt cgtacccccc agaaaatact gccccgatga
      721 aatctcacat acacactgtt cctgaagtta ctgagagtag tcaggaaccg tcaatcagcc
      781 gttcggttga gcggtgcacg ccgttccgcc agtcacgttc ccttgaacgc tcgtggccac
      841 tggcgacgct tccggctcgg tcaaaatctt ggtgatttcg ctcgctccag cgctcgcggt
      901 caactgtact gcaacgatgc gggtggccgt cctaggaacg tcaataacgc cacgaattag
      961 tctccatcca ccttccattt gggccgaaac cgccgctggc tgacccgccg cggacaccga
     1021 cttcacgccc caaaggggcg acaggtacag cgaataagcg agcttcatcg tggtgatgtc
     1081 gagcccgctg aggtgagttc cggcgtacca cgggatgtcc agtggggcgg tgctacgcag
     1141 cgtaatcgtc agcagcggcc ccgggctgca gacgttcgtc gacgtgaaac tcgtggtcag
     1201 gtaagcgccg agcttcgacc cgtcgaggct gttcagcgcc agccgtaccg tggtcgctgc
     1261 gccaggtgcc gtggccgagc cgagcgggcc accaacgccc atctccaccc acttggcttc
     1321 cgccgtcggg tccgcactcc agacctccaa gtcgccggtc cccgcgctct gggcgatctt
     1381 tcgcccaagg gcgcccaccg cgtatccggg cttcgtcatc cgcgcgaaca cggcccgggt
     1441 gacctcgccg aggaacgcga tccgcggcgc ctggtcggca cccgggaagg tgtagtactc
     1501 ctgcttctgc aagatgtcag acgcgttcgt cgcgctgagc tcggtgccat tggaggtctt
     1561 gaccggtccg gtcgcgctca acatgtggcc cagtgccacc gggtcgatgg acacgacagc
     1621 atcgggaacc acgccccgcc ccgggcccgt cgtccacgac gcgctgaaga tctgcgcggc
     1681 gtccggaaac gatgccgaga ggttgaagtt ctgcggcagc gagacgtccg agccgtagtc
     1741 ggccacgacg tcaggactca cgccgggcgg cggcaccggc accttggcta aggagtcgag
     1801 cgacccgaag gcgagcagcg agatcgcgcc cttgtctgtg cgtagttcgc cccatgcacc
     1861 gatcagtccg ccggtgcctc gctcctcggc aggggtctga aacatcagca ggatcgtccg
     1921 cggcttgtcc tggcccagca acgcgggcgc cacggtcgag agtgccgaaa ggtcctgcac
     1981 ctgggtagtc gctcgaccga gcttggtctg agcgctgacc actcgacgcg acacgaatcc
     2041 cagtaagcca ccggtgtgca gatcggcgac gtccgcgttc gccgcctcga ccgtcgtcac
     2101 ggcagccgcg atcgtcgtgc tgtcctgccc gagcgcggtg agcaggttgc tcactcctgc
     2161 gttgtgggac gcggcgacgg tgaacgtgtg cgagcggctc aacggcacga gcgcgccgtt
     2221 gacgatgcgt gacagcgact gcgcgacgac ggatgcggcg cgcaggttgc gcccgacgaa
     2281 cggaacgtgg ccggccagtt cccacatcgg gtcgtgcgag gcggacgacg cggcagtggc
     2341 gtcgttttga agccgatcgg tgtcgggggc gaaggcagtg aagcgcccgg cgccgaggtc
     2401 ggtgcgtagg taagagacgt cgacttcgga gcgggatatc gagcggtcga gctgccacgc
     2461 cttgacgcct gtccagacga ctgcgcccag cagcacaacg atgacgccga ataccgccca
     2521 cgcggcggga gggatccgcc gccagcgggg tacttcgtcg accgcaatcg gggcgaccca
     2581 ctcgtccggc tgcgcggtcg cgtccgactc ctcgggcacg tcaggctcgt cggagtcgtc
     2641 agcctgcgcg gccggatcct ccgggtcgac gcgatccccc gtcacagact cctcctcagc
     2701 acgggcgcaa gatccctctc aacagccgag acgatcactg tgagggctga ccggcgggca
     2761 gcaactcccg cgcgagagcg c
//
LOCUS       DATCBJ010000084         2336 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_65075, whole genome shotgun sequence.
ACCESSION   DATCBJ010000084 DATCBJ010000000
VERSION     DATCBJ010000084.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2336)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2336)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2336
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_65075"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..767
                     /locus_tag="VII50_01800"
     CDS             <1..767
                     /locus_tag="VII50_01800"
                     /inference="COORDINATES: protein motif:HMM:NF014801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HEY5049605.1"
                     /translation="TTVGSHDTASAVVGVPAVVSNFGYISCGTWSLVGLELDQPVLTD
                     AARSANFTNEAGVDGTTRFLRNVMGLWLLQECVRTWNSLGLPADLETLLVEAGRVPPF
                     TAVIDPDDPVFLPPGDMPARIANACERRGQTPPQSQAQTVRCILDSLALAYRSAIREA
                     QQLSGRAVEVLHIVGGGARNALLCQLTADACAVPVVAGPVEAAAIGNVLVQARALGAV
                     TGSLTELRQLVCDTMELHRYEPRGSQAAWDAAAARL"
     gene            complement(869..1114)
                     /locus_tag="VII50_01805"
     CDS             complement(869..1114)
                     /locus_tag="VII50_01805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5049606.1"
                     /translation="MEETTNEMMNEMTYDETEDLMMSQDEGTQAEIATIREAYAEKIN
                     AAIGADDDKLAIELSRQFRDEAFEAIFGENARKRDVA"
     gene            complement(1333..>2336)
                     /locus_tag="VII50_01810"
     CDS             complement(1333..>2336)
                     /locus_tag="VII50_01810"
                     /inference="COORDINATES: protein motif:HMM:NF012692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEY5049607.1"
                     /translation="TRTGVPGPAAILYEVRPEAGFVLGLDVGREYLRGAIADLTGTIR
                     SKSSPRSTATEGHGRVVELVALGKALLADAGLTPSDITQTVIGSPGVYDPKRDALALA
                     GDLPGWDSPAVLVDLREAFGSTVMVENDIDVAALAERAHGHGRNVDSFAFISVGTGLG
                     MGLVLGGKLHRGAHGAAGEIGYMPFSEGHGADVEDARQRGSFEAASTAAAVVRAATRG
                     GVPNATSAQQVFSAAAHGNSAAAAVVAEEALLVAKAVCAVITVVDPDLVVLGGGIGQA
                     LGFMAAVQANLATMAPVLPTVKTSALGADAVVQGCVAAGLDRAWELVIAGIPAPPSE"
BASE COUNT          365 a          767 c          798 g          406 t
ORIGIN      
        1 tcacgacggt cggctcgcac gacacggcct cagccgtcgt tggagtgccg gcggtggttt
       61 cgaacttcgg gtacatctcg tgcggcacct ggtcgctggt tgggctcgaa cttgaccagc
      121 cggtactgac ggacgccgcg cggtcggcga acttcaccaa tgaggcaggg gtcgacggca
      181 ccacccgatt cctgcgcaac gtcatggggc tgtggctgtt gcaggaatgc gtgcggacct
      241 ggaattccct cgggctgccg gccgatctcg aaacattgct cgtggaggcg ggccgcgtac
      301 cgccgtttac ggcggtcatc gatcccgacg atccggtgtt cctgccgccc ggtgacatgc
      361 cggcgcgcat cgcgaatgct tgcgagcggc gcggacagac gccaccgcaa agtcaggcgc
      421 agaccgtgcg gtgcatcctc gacagcctgg cattggctta ccgatccgca atacgcgagg
      481 cacagcagct gagcggccgc gcggtcgagg tgctccatat cgtgggtggc ggcgcccgca
      541 acgcgctgct gtgccagctc acggccgatg cgtgtgcggt acccgtcgtt gccggcccgg
      601 tcgaggcggc cgccatcggc aatgtgctgg tgcaggcgcg cgcgctcggc gcggtgaccg
      661 gatcgctgac ggaacttcgt cagctggttt gcgacaccat ggaactccac cgctacgagc
      721 cgcggggctc acaagcggca tgggacgcgg cagcggcccg gttgtagggg acgtggcagc
      781 ggcccggttg taggggacgc ggcagccgcg cggttgctgg cacgcggcag ccgcaccgtt
      841 gtattgggaa atgaagaaaa gttggtgact aggcgacgtc gcgcttgcgc gcattctccc
      901 cgaatatggc ctcgaaagcc tcgtcgcgga attgccgcga cagctcaata gcgagtttgt
      961 cgtcatcggc accgatcgcc gcgttgatct tctcggcgta ggcctcgcga atcgtggcga
     1021 tctccgcctg ggtgccctcg tcctgcgaca tcatcaggtc ttcagtctcg tcgtaggtca
     1081 tctcgttcat catctcgttc gtcgtctctt ccatctttag gccctccttc gttgctcagc
     1141 ccgagctatc actctgccag tggtatcggc agccaggggc cgtgcacgcg ttactaacgg
     1201 gaaaatactg tgtgacattc cccgcaaatg tgcggcccca gttgttcatc attagcggag
     1261 cctcaaatgg ctggtaattc gcatttggaa atcgcgggtg tcggtgacgt atgtctcatt
     1321 ttgcgggcag catcattctg acggcggggc ggggatgccc gcgatcacca gttcccacgc
     1381 gcggtcgagt ccggcggcca cgcagccctg caccaccgcg tcggcaccaa gggcgctcgt
     1441 cttcacggtg gggagtaccg gtgccatcgt ggcgaggttg gcctgcacgg cggccataaa
     1501 accgagcgcc tggccgatcc cgccgccaag tacgacaagg tcggggtcaa ccaccgtgat
     1561 gaccgcgcac accgccttgg cgactagcag cgcttcctcg gcgacgacgg cggcggccgc
     1621 gctgttgccg tgcgctgcgg cgctgaacac ctgctgggcc gaggttgcgt tgggcacgcc
     1681 gccgcgggtg gctgcacgga ccaccgctgc tgcggtggac gctgcctcga aactgccgcg
     1741 ttgacgagcg tcctcgacgt cggcgccatg gccctcggag aacggcatat agccgatctc
     1801 gccggccgcg ccgtgcgccc cacgatgcag cttgccgccg aggaccaagc ccatgccgag
     1861 gccggtgcca acggagatga acgcgaagct gtcgacgttg cgcccgtgcc cgtgcgctcg
     1921 ctcggcgagc gcggccacgt cgatgtcgtt ctcgaccatc acggtcgacc cgaacgcctc
     1981 gcgaagatcg accagcacgg cgggggagtc ccagccgggc aggtcaccgg ccagcgcgag
     2041 cgcgtcacgc ttcgggtcgt agacgccggg actgccgata acggtctggg taatgtcgga
     2101 cggcgtgagg cccgcgtccg cgagcagcgc cttcccgagc gcaaccagct ccacgacgcg
     2161 gccgtggcct tcggtggcgg tggatcgagg cgaggacttg ctgcgaatgg tgccggtcag
     2221 gtcggcgatc gcgccgcgca ggtattcgcg accgacgtcg agaccaagga cgaacccggc
     2281 ctctggccgc acctcgtaaa ggatcgccgc aggccctggc acgccggtgc gggtgc
//
LOCUS       DATCBJ010000085         2986 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_64979, whole genome shotgun sequence.
ACCESSION   DATCBJ010000085 DATCBJ010000000
VERSION     DATCBJ010000085.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2986)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2986)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2986
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_64979"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..574)
                     /locus_tag="VII50_01815"
     CDS             complement(<1..574)
                     /locus_tag="VII50_01815"
                     /inference="COORDINATES: protein motif:HMM:TIGR03083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maleylpyruvate isomerase N-terminal
                     domain-containing protein"
                     /protein_id="HEY5049608.1"
                     /translation="MADASRLRAILADGDLARDIAGCPGWDLAELGQHVGGIYRFATA
                     GIEGRAIDEPTGPRERLEMLEWFDAGLAPLLAVLEPRDWTRACWTFAPPHRVGFWIRR
                     MCHETALHLWDAETSQGSSAGLDPLVAPDGVDEVVTMFFPRQVRLRRISALRDCVQIV
                     LSDQVGAPAMLIHGDGSAPSDAIVPDAVVRG"
     gene            complement(876..1286)
                     /locus_tag="VII50_01820"
     CDS             complement(876..1286)
                     /locus_tag="VII50_01820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5049609.1"
                     /translation="MIVDDFLGLMFVGGLVGTVIGLVPLEFLPGWALRQWHRGVWAAL
                     FALASSGVVDLLLLNHHRQHASSRSSIVTTVVLFVLFAGGSVVFREHFIRMRRAATDA
                     PQGFLAKFRELISPAPPAIATPAPAPPVADAPAS"
     gene            complement(1354..1611)
                     /locus_tag="VII50_01825"
     CDS             complement(1354..1611)
                     /locus_tag="VII50_01825"
                     /inference="COORDINATES: protein motif:HMM:TIGR02276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5049610.1"
                     /translation="MARQSAAAPAAAVYVTNSGDATISAIDADTDAVVATIPVGQRPW
                     AVTVSSDGTTAYVSDSLSAYVSVLTCARARFADCRCRSASL"
     gene            complement(1830..>2986)
                     /locus_tag="VII50_01830"
     CDS             complement(1830..>2986)
                     /locus_tag="VII50_01830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016337099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HEY5049611.1"
                     /translation="TGTVDEPAGVPVFAWKGESLEEYWWCTEQVLLWPEGDGPNMILD
                     DGGDATLLVHKGVEFEKAGEVPDPSTADSDEFEVILRLLQRSLVEDPRRWTAIASGLK
                     GVTEETTTGVHRLYQMQETGSLLFPAINVNDSVTKSKFDNKYGCRHSLIDGINRATDV
                     LIGGKVAVVCGYGDVGKGCAESLRGQGARVIITEVDPICALQAAMDGYQVATLEDVVS
                     TADIFVTATGCLNVITADQMSRMKHQAIVGNIGHFDNEIDMAGLAKLPDVTRINIKPQ
                     VDEWMFADGHGIIVLSEGRLLNLGNATGHPSFVMSNSFTNQVLAQIELYTKTAQYPVG
                     VYVLPKYLDEAVARLHLSALGVRLTELTPAQAGYLGVPIEGPYKADAYRY"
BASE COUNT          586 a         1021 c          916 g          463 t
ORIGIN      
        1 gcccacgcac gacggcgtcc ggaacaatcg catcgcttgg agccgagcca tctccgtgaa
       61 tgagcatcgc tggagccccg acctggtcgc tcagcacgat ctgcacgcag tcacgcaggg
      121 ccgagattcg tcgaagccgg acctggcggg ggaagaacat cgtgacgacc tcatcgacac
      181 cgtcgggcgc gacgagagga tcgagtcccg cgctactgcc ctgtgacgtc tcggcatccc
      241 acagatgaag cgcagtttca tgacacatcc gccggatcca aaaaccaacg cgatgcggcg
      301 gcgcaaacgt ccagcaagcg cgggtccagt cgcgcggttc gagcaccgcg agtagcggcg
      361 cgagcccggc gtcgaaccac tccagcatct cgagccgttc tcgcggtccg gtcggctcat
      421 ctatggctcg accctcgatg cctgcggtcg caaagcgata gatcccaccg acgtgctgcc
      481 ccaactccgc gagatcccag cccgggcagc cagcgatgtc gcgcgcgaga tcgccatcgg
      541 ccaggatcgc ccgcagccgc gaagcatccg ccattagccg ggcatgatgc tccgtgttgt
      601 ccacagcgat ccatccctcc caatttgctg cgcctcgcgt tcccggcgat tcttccggca
      661 cgggggctca aggtccacgg catattctcg gagcggcaac ctgccgaagc ggcaacttct
      721 cacagcggca acttcccgca gcaagggcct cgtagcagaa ggcaagatcg aagcccccga
      781 tagcgtttgt ctcgcagatc gcgagcgcgc gctccgcgtg gaacaaaggc ggctcgacgc
      841 ggcgaagcgt ggcgacagac cttccgttcg aagactcacg aggcgggcgc atcagctacg
      901 ggcggcgcag gtgcgggtgt tgcaatcgcg ggcggtgccg gcgagatgag ttcccggaac
      961 tttgcaagga aaccctgcgg cgcatcggtg gcagctcgac gcatgcgaat gaagtgctcg
     1021 cgaaagacca ccgagcctcc cgcgaacagc acgaacagca ccaccgtggt gacgatcgag
     1081 ctcctgctgc ttgcgtgttg ccgatggtgg ttgagcagca ataagtcgac aacgccggaa
     1141 cttgccagcg cgaacaatgc cgcccagact ccgcgatgcc actgccgaag cgcccaaccc
     1201 ggcaggaact ccaacggcac cagcccgatg actgtcccga cgagaccacc gacgaacatc
     1261 aaaccgagga agtcgtcgac aatcacgagt ccggcgaacg cacgggcttc tgacgtcgac
     1321 gtaaaggaat cggccgtccg ggctcagcac agatcacaag gacgctgacc gacaacgaca
     1381 gtcggcgaac cgtgcgcgtg cgcatgtcaa gacgctgacg tacgccgaca gcgaatcaga
     1441 gacgtacgcc gtcgtgccgt cgctgctgac cgtcacggcc catggacgct gaccgaccgg
     1501 aatcgtcgcg acgacagcgt cagtgtccgc gtcgatcgcc gagatggtgg cgtcgcccga
     1561 gttcgtcacg tacacggcag ccgcgggcgc ggcagccgat tgcctggcca atgccagaac
     1621 cgaagcccgc cccgacgcca caccgaccac gagcaggccg cccgcgaacg tgattcgcgc
     1681 gccggcggct cacagctcca tccgcacatc ttcacggcgg acgagccccg cgacaaggtc
     1741 atgccgcgag cccaactgtc aaacgctggc gggtctagag acccgcaact gtctgacagt
     1801 cgacgagacc cgacagcaga cccgacagat cagtagcggt aggcgtcagc cttgtacggg
     1861 ccctcgatcg ggacgcccag atagccggcc tgcgcaggcg tgagctccgt cagccgcact
     1921 ccgagcgccg acaggtgcaa gcgggcaacc gcctcgtcga ggtacttggg cagcacgtag
     1981 acgccgaccg ggtactgcgc ggtcttcgtg tatagctcga tctgcgcgag cacctgattg
     2041 gtaaacgagt tgctcatcac gaagctcggg tgcccggtag cgttgccgag gttcagcagc
     2101 cgaccttcgg acagcacgat gatgccgtgc ccgtcggcga acatccactc gtcgacctgc
     2161 ggcttgatgt tgatgcgcgt cacgtcgggc agcttcgcaa gaccggccat gtcgatctcg
     2221 ttgtcgaagt ggccgatgtt accgacaatc gcctggtgct tcatacgcga catctggtcg
     2281 gcggtgatga cgttgaggca gccggtcgca gtgacgaaga tgtcggccgt cgacacgacg
     2341 tcctcgagcg tggcgacctg gtagccgtcc atcgcggcct gcagtgcgca gatcgggtcg
     2401 acctcggtga tgatgacgcg cgcgccctgg ccacgcagcg actccgcgca acccttgccg
     2461 acgtcaccgt agccacacac cacggcaacc ttgccgccga tcagcacgtc ggtcgcccgg
     2521 ttgatgccgt cgatgagcga gtgccggcag ccgtacttgt tgtcgaactt cgacttggtc
     2581 accgagtcat tcacgttgat tgccgggaac agcagcgaac cggtctcctg catctggtag
     2641 agccggtgca cgccggtcgt ggtctcctcg gtcacaccct tcagacccga agcaatcgcc
     2701 gtccaacgtc gtgggtcttc gacgagcgag cgctgcagca gccgcagaat gacttcgaac
     2761 tcgtcggagt cggccgtcga cgggtcggga acctcgccag ccttctcgaa ctcgacgccc
     2821 ttgtgcacga gcaacgtcgc gtcgccgccg tcgtccaaaa tcatgttcgg accgtcaccc
     2881 tcaggccaca acagcacctg ctcggtgcac caccagtact cctcgagcga ctcgcccttc
     2941 caggcgaaca caggcacgcc ggctggctca tcgacagtgc ccgtcg
//
LOCUS       DATCBJ010000086         3535 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_26810, whole genome shotgun sequence.
ACCESSION   DATCBJ010000086 DATCBJ010000000
VERSION     DATCBJ010000086.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 3535)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3535)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3535
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_26810"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..530)
                     /locus_tag="VII50_01835"
     CDS             complement(<1..530)
                     /locus_tag="VII50_01835"
                     /inference="COORDINATES: protein motif:HMM:NF012742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEY5049612.1"
                     /translation="MTVSNAYNRPDQLSEQTRARVLAVAKELGYAGPDPAGRSLRTRR
                     ADTIGVLLTEQLPYAFADPGMVSFLHGLATGLADVGQALLLVPTEGNAEHGHVRNALV
                     DGFVLTSLVAGHPAVADVLGRRLPVVSWGHPRIPGVPRISVDNARAAAKAARHLLELG
                     HRSFGVVTFGDSAGPVG"
     gene            718..1347
                     /locus_tag="VII50_01840"
     CDS             718..1347
                     /locus_tag="VII50_01840"
                     /inference="COORDINATES: protein motif:HMM:NF016109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQ-loop domain-containing transporter"
                     /protein_id="HEY5049613.1"
                     /translation="MTGHEIAFGLGYLGAALGVAMVVPQIVRIVRHPSLPGVSPISWA
                     LTVFACLLWMTYGLRSGALPQIPGNVLLVCGAIAVVLLVKTRTSRARRAVLLTLGAGL
                     LEATAWVIPPQGVGYLAFAIGTVSTWPQLYDSVGNWRAHLNSGVSLSTWGLRIASQVC
                     WLSYAVGTSDLPVGIGACVAGLTGIAMVALETAARAPAFGSGGVMTETA"
     gene            1437..2123
                     /locus_tag="VII50_01845"
     CDS             1437..2123
                     /locus_tag="VII50_01845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5049614.1"
                     /translation="MAGAQIRSLAAVAIVAIAGFSLTACSKSSNPKAVTPNVTAPSQA
                     AQGNGGGAASSPGGGGGGGGGGSAAAASAAAGGNTLLANGPACQILTAADVQAAVGMP
                     LGDITGDISGGPQGATNYHSCIWTKDVTGEGAAVNVEYGTYASASSQLATQKATDTDS
                     ANQLNSLSPNSQTIKDVSVGDGGYELTTSAGGDDTITFTKGQQLIQVEVIKGVPGAAM
                     TIAQKVASKL"
     gene            2166..>3535
                     /locus_tag="VII50_01850"
     CDS             2166..>3535
                     /locus_tag="VII50_01850"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012789.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HEY5049615.1"
                     /translation="MFDFLAELNPEQRAAATFETPVPLRIVAGAGTGKTTALASRVAW
                     LLASGTPPERVLLLTFTRRAARQLLARTETMLANAPTARRVRNPGRVQGGTFHSVAHR
                     TLRHHAPRLGLPDGFSVLDTSDAADLFDLVRDQQLGTSTARRRLPRKALLLDLYSRAV
                     NTGRPLSEVISVVAPWCTELTESVAAMCREYVVRKRALGLVDFDDLLLYWRAAALDPM
                     VGPQLAGEFDHILVDEYQDVNALQVDVVAALRSSDRRLTVVGDDAQAIYGFRAADPAH
                     LLDFELAFPDATTITLETNYRSSPQILSAANAVGAQAPAGFRAILRATEPERSNGNRP
                     QLLRCADEDAQSNAVCDRVLEHREAGLRLRDQAVLFRAAHHSEHLELELSRRAIPYVK
                     YGGLRYLEAAHVKDLLAAFRLADNTRDEVSWFRLLQLLDGVGPATARRVIDALALVQN
                     KTEADA"
BASE COUNT          582 a         1193 c         1166 g          594 t
ORIGIN      
        1 ccgaccggac cggcactgtc gccgaaggtc accaccccga aactcctgtg cccgagctcg
       61 agcagatgtc gcgcggcctt ggccgcggcg cgcgcgttgt ccacgctgat ccgtggcaca
      121 ccggggattc gcggatggcc ccacgacacc accggcagcc tgcggccgag gacgtcggcc
      181 accgccggat gcccggcgac cagcgatgta aggacgaacc cgtcgaccag cgcgttgcgc
      241 acgtgcccat gttctgcatt tccctcggtc ggaaccagca acaatgcctg gccgacgtcg
      301 gcgaggccgg tggccaagcc atgcaggaac gacaccatgc cggggtcggc gaaggcgtag
      361 ggcaattgct cggtcagcaa caccccgatg gtgtcggctc ggcgggttcg tagcgagcgc
      421 ccggccgggt cggggccggc gtatccgagc tccttcgcga ccgcgagaac ccttgctctg
      481 gtctgctccg acagctggtc tggccggttg taggcgttcg acaccgtcat cgccgatacg
      541 ccagcgactt gggcgacggt ttccagagtc gggcgtcctg gggttatgag ggcgctccct
      601 tctctggtgc tgtatcgtta cagaaaagac gtcgatatgc cgaggtacca gctagcaacg
      661 gatgatcgcc cgtgatcgcc gtcccgtctg aggagtatgc acggtgggtg tacggcaatg
      721 accgggcacg agattgcgtt cgggctgggc tacttgggag ccgcgctggg cgtcgcgatg
      781 gtggtccccc agatcgtgcg gatcgtgcga cacccctcac tgccgggagt ttcgccgatt
      841 tcgtgggcgc tcaccgtctt tgcctgcctg ctgtggatga cctacgggct acgaagtggt
      901 gccctgccgc agatccccgg aaacgtgctg ctcgtctgtg gtgcgatcgc ggtcgtgctg
      961 ctggtcaaga cccggacctc gcgggcccgc cgcgcagtgc tgctcaccct cggcgccggg
     1021 ctgctcgagg cgacagcgtg ggtgatcccg ccccaaggcg tcggctatct tgcgttcgcc
     1081 atcggcaccg tctccacctg gccgcagctg tatgactcgg tcggcaactg gcgggcgcac
     1141 ctgaactccg gcgtctcact gagcacctgg ggactccgga ttgcctcgca ggtgtgctgg
     1201 ctctcctacg ccgtcggcac gtccgacctg cccgtcggga tcggcgcctg cgtcgcagga
     1261 ctgacgggta tcgcgatggt cgcactcgag accgcagcca gagcacctgc gttcgggtca
     1321 ggtggcgtca tgaccgagac ggcctagctc agatcgtcta agcaattgac tgcggatacc
     1381 ggaccatctc cgccagccct aggatctcaa gaccgttggc tgctggggag gacatcatgg
     1441 cgggagccca aattcgctcg ctcgcggcag tggcgatcgt tgctatcgcc gggttcagcc
     1501 tgactgcgtg ttcgaagtcg agcaatccaa aggccgttac gccgaacgtc accgcgccct
     1561 cgcaggccgc ccaaggcaat ggcggtggag cggccagtag cccgggtggc ggcgggggcg
     1621 gtggcggcgg ggggtcggca gctgccgcat cggcagctgc aggaggaaac accctgctcg
     1681 ccaacggtcc ggcctgtcag atcttgacgg ccgccgacgt ccaggccgcg gtcgggatgc
     1741 cgttgggcga catcaccgga gacatcagcg gcggcccgca gggggctacg aactaccaca
     1801 gctgcatctg gaccaaggac gtcaccgggg aaggcgcggc cgtcaacgtc gaatacggaa
     1861 cctacgcatc ggcgtcgtca cagctcgcca cccaaaaggc gacggacacc gactcagcga
     1921 accaactcaa cagcctgtcg cctaactcgc agaccatcaa ggacgtgtca gttggggacg
     1981 gcggctacga gctcaccacg agcgccggtg gagacgacac cattaccttc accaaggggc
     2041 aacagctcat ccaggtcgag gtcatcaagg gcgtgcccgg cgcagcgatg acgatcgcgc
     2101 agaaggtcgc ctcgaagctc tgattccagg cggcccggcg gcctcaatgg gctacagtcg
     2161 aacatgtgtt cgacttcctc gctgaactaa accccgaaca gcgggcggcg gcgactttcg
     2221 agacgccggt gccactacgg atcgtggccg gagccggcac cggcaagacg acggcgttgg
     2281 ccagcagggt ggcatggctg ctcgcgtctg gaacgccgcc cgagcgcgtc ttgctgttga
     2341 cctttacccg ccgggcggct cgacagttgc tggctcgcac ggagaccatg ctcgccaacg
     2401 cccctaccgc gcggcgggtg cgaaacccgg gtcgcgttca gggcggcact ttccactcgg
     2461 tggcccaccg cacgttgcgc caccacgccc cccggctcgg cctccccgac ggcttctcgg
     2521 ttctcgacac gtccgatgct gccgacctct tcgatctcgt ccgcgaccag caattgggga
     2581 cgtcgaccgc gcgccgcagg ctgcctcgca aagcgctgct gctcgacctc tactcgcgag
     2641 cggtgaacac cggccggcca ctgtccgagg tgatctccgt ggtcgcgccg tggtgcacag
     2701 agctgaccga atcggtcgcc gccatgtgca gggagtacgt cgtccggaag cgcgcgttgg
     2761 ggctcgtcga cttcgacgac ttgctgctct actggcgcgc cgctgcgctc gacccgatgg
     2821 tcggcccgca actcgcgggc gagttcgacc acatcctggt cgatgagtac caggacgtga
     2881 acgcattgca ggtcgacgtc gtggcggcgt tgcgcagcag cgatcgtcgc ctcaccgtcg
     2941 tcggcgacga cgcgcaggcg atctacgggt ttcgagccgc cgatcctgct cacctgctcg
     3001 acttcgagct ggcctttccc gacgcgacga cgatcaccct cgaaacgaat taccgttcgt
     3061 cgccgcaaat cctgtcagca gccaacgccg tgggtgcgca ggcgccagcg ggctttaggg
     3121 cgatcctgcg cgctaccgag ccggagcgtt cgaacggcaa tcggccgcag ctactgcgct
     3181 gcgccgacga agacgcgcaa agcaacgctg tgtgcgatcg cgtgctcgag catcgcgagg
     3241 ctggcctccg gctgcgcgac caggctgtgt tgttcagggc cgcgcaccac tccgaacacc
     3301 tcgaactcga gctgagccgg cgcgcaattc cgtatgtgaa gtacggaggc ttgcgctatc
     3361 tcgaagccgc ccacgtcaag gatctcctcg ctgccttcag gctcgctgac aacactcgcg
     3421 atgaggtgtc gtggtttcgg ctgttgcaac tcctcgacgg cgtcggacca gcgacggcgc
     3481 gtcgcgtcat cgatgcgttg gcccttgtcc agaacaagac cgaggcggac gccga
//
LOCUS       DATCBJ010000087         3048 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_26757, whole genome shotgun sequence.
ACCESSION   DATCBJ010000087 DATCBJ010000000
VERSION     DATCBJ010000087.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 3048)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3048)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3048
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_26757"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            82..1431
                     /locus_tag="VII50_01855"
     CDS             82..1431
                     /locus_tag="VII50_01855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020661760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuconate dehydratase"
                     /protein_id="HEY5049616.1"
                     /translation="MPIVQSVDVVDVRFPTSLDRDGSDAMNKDGDYSAAYVMLSTDDP
                     ELAGFGLTFTIGRGNELCVAAARQQAALLVGMDVDVMAADMGAVYRVLTADSQLRWLG
                     PEKGVVHLSLAAVLNAAWDLLARRAQVPLWRLLAGMSAQQLAAVADLRYVSDVLTRAE
                     AESMLAALEPTRADRIALLERDGYPCYTTSPGWLGYSDEKLTRLCAEAVAAGFSYIKL
                     KVGANLEDDRRRCGIARGILGPDRHLMIDANQVWDVGQAIEWVNALADFEPLWIEEPT
                     SPDDILGHAAIRAGVRPVGVATGEHAHNRVMFKQFLQAGAIDYAQIDSCRLASVNEII
                     TVLLMAAKFGVPVCPHAGGVGLCELVQHLSIFDYVAVSGSLDKRVIEYVDHLHEHFTD
                     PCVIKNAAYMLPTAPGYSSRMHSGSVSTYRYPDGAYWQQRLKQVGLDVPAQPPAYWI"
     gene            1582..1878
                     /locus_tag="VII50_01860"
     CDS             1582..1878
                     /locus_tag="VII50_01860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5049617.1"
                     /translation="MAGAIARSLDAADTAAFWADVGRTMGASDAGSAASSDVEQLSGS
                     LTAGLDPDETWEDVWAVAETSTSATSARRSVTSRDSAGPLSSSPTTGSIDMACP"
     gene            1934..2074
                     /locus_tag="VII50_01865"
     CDS             1934..2074
                     /locus_tag="VII50_01865"
                     /inference="COORDINATES: protein motif:HMM:NF014505.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5049618.1"
                     /translation="MLPVVSYVQCELIRAVSADRLIRPVASLDTAHIANIEAILRRIL
                     DL"
     gene            complement(2076..>3048)
                     /gene="guaA"
                     /locus_tag="VII50_01870"
     CDS             complement(2076..>3048)
                     /gene="guaA"
                     /locus_tag="VII50_01870"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HEY5049619.1"
                     /translation="EPSWTTAGIVADQVARIRSQVGSGRVICGLSGGVDSAVAAALVQ
                     RAVGGQLTCVFVDHGLLRKGEAEQVERDFVASTGVRLHVVEASAIFLAALAGVSDPES
                     KRKIIGREFIRAFEVAAREISATTPVDFLVQGTLYPDVVESGGGSGTANIKSHHNVGG
                     LPDDLQFALVEPLRTLFKDEVRAVGAELGLPESLVWRQPFPGPGLAVRVIGEITAERL
                     AVLREADAIAREELTASGLDRDIWQCPVVLLADVRSVGVQGDGRTYGHPIVLRPVSSE
                     DAMTADWSRVPFGVLARISTRITNEVREVNRVVLDITSKPPGTIEWE"
BASE COUNT          558 a          983 c          954 g          553 t
ORIGIN      
        1 ctgcgcgccc gaatcgtttg acctttgcga accctgagcc taacgttgcg cgttacatcc
       61 gatcactttc tggggtttct cgtgccgatt gttcagtccg tcgacgtggt cgacgtgcga
      121 tttccgacgt cgctcgaccg cgatggttct gatgcgatga acaaggacgg ggactactcg
      181 gcggcatacg tgatgctgtc gaccgacgac cccgagctgg cgggtttcgg gttgaccttc
      241 acgatcggcc ggggcaacga gttgtgcgtc gcggcagcgc ggcagcaggc ggcgttgctg
      301 gtggggatgg acgtcgacgt gatggcggct gacatgggtg cggtctaccg ggttctgacg
      361 gccgactcgc aattgcgctg gttgggtccc gagaaggggg tcgtgcacct gtccttggcg
      421 gcagtactga acgcggcctg ggatctgctc gcacggcgag cgcaggtgcc gctctggcga
      481 ctgttggcgg ggatgtcggc gcagcagcta gctgcggtgg ctgatcttcg ttacgtctct
      541 gacgtgctga ctcgcgcgga ggctgagtcg atgttggcgg ccttggagcc aacccgagca
      601 gaccgcatcg cgctactgga gcgagacggt tacccctgtt acacgacctc tccgggctgg
      661 ctcggctaca gcgatgagaa gctgacgcgg ttgtgcgcag aggctgtggc ggcggggttc
      721 tcctatatca agctgaaggt tggtgcgaat ctcgaagacg atcgacggcg atgcggcatc
      781 gcgcgcggga ttctcgggcc ggatcgccat ctcatgatcg acgcgaacca ggtgtgggac
      841 gtcggtcagg cgatcgagtg ggtcaacgcc ctcgccgact tcgagccact gtggatcgag
      901 gagccgacca gccccgacga catcttgggc cacgctgcga tccgcgccgg tgtgcgcccg
      961 gtgggtgtcg cgaccggcga acacgcccac aacagggtga tgttcaaaca gttcctgcaa
     1021 gccggcgcga tcgactacgc acagatcgac tcgtgccggt tggccagtgt caacgagatc
     1081 atcacggtgt tgttgatggc cgccaagttc ggcgtcccgg tctgcccgca cgcaggcggc
     1141 gtcgggcttt gcgagctcgt acagcacctg tcgatcttcg attacgtagc ggtttccggc
     1201 agcctcgaca agcgagtcat cgagtacgtc gaccacctcc atgaacactt cacggacccg
     1261 tgcgtaatca agaacgccgc gtacatgctc cccaccgccc ccggttacag ctcacgtatg
     1321 cactccggct ccgtcagcac ctaccgctac cccgatggtg cctactggca gcaacggctc
     1381 aagcaggtcg ggctcgacgt gcctgcgcag ccgcccgcct actggattta agacaggtgg
     1441 cccggctcga tccgggtaga cagttgggta cacaccggtg gaaggaaagg tatacccgct
     1501 ggtatgccgt ctacgacgat caaggttccg gtcgagttga gagatcgcct tgcctcgctc
     1561 gctagccgtg aaagcacaac gttggccggc gcaatcgccc gatcgctgga cgcagcagat
     1621 accgccgcgt tctgggctga tgtcgggagg acgatgggcg catcggacgc cggatcggcg
     1681 gcctcctcgg atgtcgagca actcagtggc agcctcacgg ccgggctcga tccggacgag
     1741 acctgggagg acgtgtgggc agtcgcggag acatctacct ctgcgacttc ggcacgccga
     1801 tcggtcacga gccgagattc cgcaggcccg ctgtcgtcgt ctcccacaac gggctcaatc
     1861 gacatggcgt gcccatagtg ctgccggtta cgcgggctcg taggggctat ccgacacacg
     1921 tgagttggac agcgtgctgc cggttgtcag ctacgtgcag tgcgagttga tacgagccgt
     1981 gtcagccgat cgcctgatcc gaccggtcgc atcgttggat accgcgcaca tcgccaacat
     2041 cgaggcgatc ttgcgacgca tccttgacct gtgacttact cccattcgat agtgccgggc
     2101 ggcttgctgg tgatatcgag cactacccga ttgacttcgc gcacttcgtt cgtgatccgc
     2161 gtcgagattc gcgcgagtac gccgaacgga acccgtgacc agtcggccgt cattgcatcc
     2221 tccgacgaca ccggcctcaa cacaatcggg tgaccatagg tgcggccgtc gccctgcact
     2281 ccaaccgacc ggacgtcggc cagcagcacg accggacatt gccagatatc gcgatccaga
     2341 cccgacgcgg tgagttcctc gcgcgcaatc gcgtcggctt cgcgtaacac cgccagccgc
     2401 tcggccgtga tttccccgat gacgcgcacc gcaaggccgg gaccaggaaa aggctgccgc
     2461 cacaccaagg attccggcag accaagttcc gcgccgaccg cgcgtacctc gtccttgaac
     2521 aaggtccgca acggctcgac gagagcgaac tgcaggtcgt cgggtaagcc accaacgttg
     2581 tggtgtgact tgatatttgc ggtgccgctc ccgccgcccg actccacgac gtccggatac
     2641 aacgtgccct gaaccagaaa atcaaccggc gtcgtggcac tgatctcacg cgccgcaacc
     2701 tcgaacgcgc ggatgaactc gcggccgatg attttgcgct tcgattcggg atcactcacc
     2761 ccggcaagtg cggctagaaa aatagccgac gcctcgacaa cgtggagacg aacacccgtc
     2821 gaagcaacga aatcccgctc cacctgctcg gcctcgccct tacgcagcaa accgtggtcg
     2881 acgaacacgc aggtgagctg gccgcccacc gcacgttgca ccaaagctgc cgcaacggcc
     2941 gagtcgacac caccagacag tccgcagatc acccggcccg atcccacctg cgaacggata
     3001 cgcgcaactt gatccgcgac gatccccgcc gtcgtccaac ttggttcg
//
LOCUS       DATCBJ010000088         2598 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_322723, whole genome shotgun sequence.
ACCESSION   DATCBJ010000088 DATCBJ010000000
VERSION     DATCBJ010000088.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2598)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2598)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2598
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_322723"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..305)
                     /locus_tag="VII50_01875"
     CDS             complement(<1..305)
                     /locus_tag="VII50_01875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5049620.1"
                     /translation="MKSVALGIGLCVIAIALGGSLWYWRRGSRGLGTPLQRTTYDVLH
                     TAGSAARGLRAGLTRESAARSAPALRALLAARAMAITSTSGLLAWDGPGIDHAPSVLV
                     "
     gene            486..2459
                     /gene="acs"
                     /locus_tag="VII50_01880"
     CDS             486..2459
                     /gene="acs"
                     /locus_tag="VII50_01880"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020664180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003987 - acetate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019427 - acetyl-CoA biosynthetic process
                     from acetate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HEY5049621.1"
                     /translation="MPDPTIEALFDESRSFPPPDHFAGPSTVTSEHIYDEAKDSQGWW
                     AAEAEKVVWRKPWDQVLDWSKAPFAKWFVGGRLNITETCLDQHLAEHGDRVAYYWEGE
                     PGDTRALTYADLHAEVCRLANALTELGLQPDDRVAIYMGMVPELPAALLACARIGAPH
                     SVIFGGFSAEALRDRIVDAQAKVLITCDGAYRNGGVIPLKEWADEAVSQTPSIEKVLV
                     VKRTNNDVQMQEGRDVWYHDVVPQQLATFTPTDRDSEDLLYMLYSSGTTAKPKGIVHT
                     TGGYLVGTASTFRMVFDIRPETDIYWCTADIGWVTGHSYIVYGPLANGATSVMYEGVP
                     QFPDKDRIWDMVERYKVNILYTAPTAIRTMVRWGNEYPERHDLSSLRLLGSVGEPINP
                     EAWIWYQTVIGHGNCPIVDTWWQTETGQILLSPLPGLTATKPGSACRPLPGITADVVD
                     NEGNSVPLGGAGYLVITQPWPAMLRTLFNDPDRYVDTYWRRFSKPEDDRWIYFPADGC
                     KRDAEGYYWLLGRVDDVMNVSGHRISTLEVESALVDHPSVAEAAVVGRNDARTGQAIA
                     AFVSPKSGVVADEALIQELRDHVAKKIGKIARPSSIVFTDELPKTRSGKIMRRLLRDV
                     SEQRQLGDVTSLANADVVRDIAERAKSMVTQEE"
BASE COUNT          489 a          863 c          829 g          417 t
ORIGIN      
        1 accaagaccg acggcgcatg gtcgattcct gggccgtccc aggcgaggag cccgctggtg
       61 ctggtgatcg ccatcgcccg cgccgcgagc agcgcgcgca gggcaggcgc cgaccgcgcg
      121 gcactctcgc gcgtcagacc ggcccgcagc ccgcgcgcgg ccgacccggc ggtgtgcagc
      181 acgtcgtacg tggtgcgctg caatggggtg cccaggccgc ggctgcctcg ccgccagtac
      241 cacagcgagc cgccgagggc gatcgcgatc acgcaaagac cgatgcccag cgctaccgac
      301 ttcacgcccc cgacagtagg ccgggaagtc ggttggttaa agaggcgcgc gtgaccactg
      361 ggcgatcact gccaccgttc ggcgcgcaaa aagatccgct cggcgcgcca cgaccagaca
      421 gacgttgcca agaactgcaa ccgctcccag gctctgcgca gaactgatct tctgggaggc
      481 acccgatgcc cgatccgacc atcgaagccc ttttcgacga gtcgcgtagc tttccgccgc
      541 ctgaccattt cgccggcccg agcacggtca cgagcgagca catttacgac gaggcaaagg
      601 attcgcaggg ctggtgggcc gccgaggcgg agaaggtcgt ctggcgcaag ccgtgggatc
      661 aggtgctgga ctggagcaaa gcgccgttcg cgaagtggtt cgtcgggggc cggctgaaca
      721 tcaccgagac ctgcctcgac cagcacctcg ccgagcatgg tgaccgggtc gcgtactact
      781 gggaaggcga gccgggcgac acgcgcgcgc tgacctatgc cgatctgcac gccgaggtat
      841 gccggcttgc aaatgcgctt accgaactcg gcctgcaacc cgacgaccgg gtcgcgattt
      901 acatgggcat ggtgcccgag ctacccgcgg cactgctcgc gtgcgcccgg atcggtgcgc
      961 cgcactcagt catcttcggc ggcttctcgg cggaggcgtt gcgcgaccgc attgtcgacg
     1021 cccaggcgaa ggtgctcata acctgcgacg gcgcctaccg caacggcggc gtgatcccac
     1081 taaaggaatg ggccgacgag gcagtttcgc aaaccccgtc catcgagaag gtccttgtcg
     1141 ttaagcgaac gaacaacgac gtgcagatgc aggaaggtcg cgacgtctgg taccacgacg
     1201 tggttccgca gcagctggct actttcacgc ccaccgatcg cgactccgag gatttgctgt
     1261 acatgctgta ttcctcgggt accaccgcca agccgaaggg catcgtccac accaccggcg
     1321 gttacttggt cggcacagcc agcaccttcc ggatggtctt cgacattcgt cctgagaccg
     1381 acatctactg gtgcaccgcc gacatcggtt gggtcaccgg ccacagctac atcgtgtacg
     1441 ggccgcttgc caacggcgcc acgagcgtca tgtacgaagg cgtgccgcag ttccccgaca
     1501 aggaccgcat ctgggacatg gtcgagcgct acaaggtgaa catcctgtac accgcgccga
     1561 cggcgatccg cacgatggtg cgctggggca acgagtaccc ggagcgacat gatctgtcgt
     1621 ccctgcggtt gctcggcagt gtgggcgagc cgatcaaccc tgaagcctgg atctggtacc
     1681 agaccgtcat aggccacggg aattgcccga tcgtcgacac ctggtggcag accgagaccg
     1741 ggcagatcct gctgtcgccg ctgcccgggc tgaccgcgac gaaacctggg tcggcgtgcc
     1801 gcccgttgcc gggcatcacc gccgacgtcg tcgacaacga gggcaactcg gtgcccctcg
     1861 gcggtgccgg gtatctagtc atcacgcagc cgtggccggc catgttgcgg accctcttca
     1921 acgaccctga ccgttacgtc gacacgtact ggcgacgctt cagcaagcca gaggatgacc
     1981 ggtggatcta cttcccggcc gacggttgca agcgtgacgc ggagggttac tactggctgc
     2041 tcggtcgggt cgacgacgtc atgaacgtct caggccatcg catctcgacc cttgaggtcg
     2101 agtccgctct ggtcgaccat ccgtcggtcg cggaagcagc cgtcgttgga cgcaacgacg
     2161 cccgcaccgg gcaggccatc gctgcattcg tgtcaccaaa gagtggtgtc gttgctgatg
     2221 aagcgctaat tcaggagttg cgcgaccacg tcgcgaagaa gatcggcaag atcgcgcgac
     2281 cgtcgtcgat cgtgttcacc gatgaacttc cgaagacgcg cagcggcaag atcatgcgcc
     2341 gtctgttgcg cgacgtctcc gaacagcgcc aactcggtga cgtgacgtca cttgccaacg
     2401 ccgacgtcgt ccgggatatc gccgaacgag cgaagtcgat ggtgacccaa gaggagtaag
     2461 agcaaagcac tgcagcacgc gtacgtgtca gatgttaacc aggacccagt tctcatccag
     2521 gaggcacagt gtccagtcag accccggccg cacccgcgcc ggcacagttc atcgccgacc
     2581 cggggccact cgggctcg
//
LOCUS       DATCBJ010000089         2151 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_211521, whole genome shotgun sequence.
ACCESSION   DATCBJ010000089 DATCBJ010000000
VERSION     DATCBJ010000089.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2151)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2151)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2151
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_211521"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..686
                     /locus_tag="VII50_01885"
     CDS             <1..686
                     /locus_tag="VII50_01885"
                     /inference="COORDINATES: protein motif:HMM:NF017050.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HEY5049622.1"
                     /translation="VQGAPQTAGRFEAEVRMYKGPPPEVSCEQFYETDPAKPYKRGFS
                     IQTVSPLPITYAEHVVAQGHWGESLREYMRDYVHWASLGALCELLPLHANAVTLSGEV
                     DRHGLPVANFHYSMGDNDKQLMAAAQTKMEEILRAAGATETMTIRRFAHLVGGCRMAR
                     SPKDGVVNAEQRTFAVPNLYITDGSVLPTQGSANPALTIMSLAARVADHLTARQHTGP
                     AAAAEARHG"
     gene            679..1605
                     /locus_tag="VII50_01890"
     CDS             679..1605
                     /locus_tag="VII50_01890"
                     /inference="COORDINATES: protein motif:HMM:NF024771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HEY5049623.1"
                     /translation="MAEVAPDRVVADDDLRVVRLNASAYTIPTDLPEADGTLAWDATT
                     LVVVELGLQAGGSGLGFAYGHQAMVEVIKQTLTPNVIGIDVRDTGAAWSAMVKAIRNL
                     GRPGLSSMAIAAVDLALWDAKARALDQPLHRLLGPVRESVPIYGSGGFTTYSMPQLLN
                     QLAGWVGAGIPRVKMKIGTDWGRSWRADVARIAAVRDAIGDDAELFVDANGGYDRVQA
                     RKLGMRFAADFGVSWFEEPVSSDDLHGLAALVDALPLDVAAGEYGYDLNYFANMLAAG
                     AVDILQADVGRCGGITEWLRVAALSAAAQLPL"
     gene            1620..1841
                     /locus_tag="VII50_01895"
     CDS             1620..1841
                     /locus_tag="VII50_01895"
                     /inference="COORDINATES: protein motif:HMM:NF024771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HEY5049624.1"
                     /translation="MPLSGHCGPAIHLHAATVPPNLRHLDYFHDHVRIENMLFDGIVQ
                     PENGALTPAVTPGNGYAFKHADAKPYRVA"
     gene            complement(1906..>2151)
                     /locus_tag="VII50_01900"
     CDS             complement(1906..>2151)
                     /locus_tag="VII50_01900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5049625.1"
                     /translation="NAAADPGRSFDEWRQALMALKVAESRGEPYATLLRRSEDVIRAR
                     SVLTHERVQAGLRVPDDVLEHLTVDELLLGQPDDRVR"
BASE COUNT          396 a          776 c          655 g          324 t
ORIGIN      
        1 tggtgcaagg cgcgccacag accgccggac ggttcgaggc cgaagtgcgg atgtacaaag
       61 ggccgccacc ggaagtcagc tgcgagcagt tctacgaaac cgatcccgcg aagccctaca
      121 agcgcgggtt ctccatccag accgtgtcac cgctgccgat cacctacgcc gaacacgtcg
      181 tcgcgcaagg acactggggt gaatcattgc gcgaatacat gcgcgactac gttcactggg
      241 catcactggg cgccctatgc gaactgctgc ccttgcacgc aaacgcggtg acactctccg
      301 gcgaggtcga ccggcacggc ctgccggtgg ccaacttcca ctactcgatg ggcgacaacg
      361 acaagcaact gatggccgcc gcccaaacga agatggaaga aatcctgcgc gcagccggtg
      421 cgaccgagac gatgaccatc cgacgattcg cccacctcgt cggcggctgc cggatggcac
      481 gctcacccaa agacggcgtc gtcaacgccg aacagcgcac cttcgccgtg ccgaacctct
      541 acatcaccga cggtagcgtg ctgccgaccc agggctcggc taacccggcg ctgacgatca
      601 tgtcgctggc cgcccgcgtc gcggaccacc tgaccgcccg ccagcacacc ggcccggccg
      661 cggccgcgga ggctcgtcat ggctgaggtg gcgcctgacc gtgtcgtggc cgacgacgat
      721 ctgcgcgtgg tgcggctgaa cgcgtcggcc tacacgatcc cgaccgacct tcccgaagcc
      781 gacggcaccc tggcctggga cgccacaacg ctcgtcgtcg tcgaactggg cctgcaagcg
      841 ggcgggagcg ggctcggctt cgcctacggc caccaggcga tggtcgaggt catcaagcag
      901 acactgacac ccaacgtcat cggcatcgac gtccgggaca ccggcgcggc ctggtccgcg
      961 atggtgaaag cgatccgcaa cctgggccga cctgggctgt cgtcgatggc gatcgccgcc
     1021 gtcgaccttg cactgtggga cgccaaagcc cgcgccctgg accagccgct gcataggtta
     1081 ctcggcccgg tgcgggagtc ggtaccgatc tatggcagcg gaggattcac cacctactcc
     1141 atgccgcagc tgttgaacca actcgccggc tgggtcggtg ccggtatacc ccgcgtcaag
     1201 atgaagatcg gcaccgactg gggtcgctca tggcgcgccg acgtcgcacg catcgctgcg
     1261 gtccgcgacg ccatcggtga cgacgccgaa ctgttcgtcg atgccaacgg aggatacgac
     1321 cgggtgcagg ccagaaagct cggcatgcgg ttcgccgccg acttcggcgt cagctggttc
     1381 gaagagcccg tcagctccga tgatctgcac ggccttgccg cgctggtgga cgcactgcca
     1441 ctagacgtcg ccgcaggcga atacggctac gacctcaact atttcgcgaa catgctcgcc
     1501 gccggcgccg tcgacatcct gcaagccgac gtcggccgct gtggcgggat caccgaatgg
     1561 ctgcgtgttg ccgctctgag cgccgccgcg cagttgccgc tctgagcgcc gccgcgcagt
     1621 tgccactctc cggccattgc ggcccggcga tccacctgca tgccgcgacc gtccccccca
     1681 acctgcgcca cctcgactac ttccacgacc acgtccgcat cgagaacatg ctgttcgacg
     1741 ggattgtcca acccgaaaac ggcgccctca caccagctgt gacgccgggt aacggctacg
     1801 cgttcaagca cgcggacgcc aagccgtacc gagtcgccta gaaccacaca tcgcgcgccg
     1861 acagacaacc acgcatcgca gccatcgcga cgctgcgcct tcctgttacc tgaccctgtc
     1921 gtccggctgg ccaagcagca attcgtcgac ggtcaggtgc tccaagacat cgtcgggcac
     1981 ccgaagcccg gcctggaccc gctcatgggt gaggacggaa cgcgcgcgga tcacatcctc
     2041 cgaacgtcga agcaacgtcg cgtacggttc gccacgactc tcagcgacct tcagtgccat
     2101 cagcgcctgg cgccactcat cgaatgaccg acccggatcg gccgctgcgt t
//
LOCUS       DATCBJ010000090         2895 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_247753, whole genome shotgun sequence.
ACCESSION   DATCBJ010000090 DATCBJ010000000
VERSION     DATCBJ010000090.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2895)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2895)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2895
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_247753"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..757
                     /locus_tag="VII50_01905"
     CDS             <1..757
                     /locus_tag="VII50_01905"
                     /inference="COORDINATES: protein motif:HMM:NF039362.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF6159 family protein"
                     /protein_id="HEY5049626.1"
                     /translation="WAALTADPTLLLVPLAVLFVGGVASAGYIAVFGGLAHLLTGGRG
                     AVAVKTLPRAVVLAVVSVIGRAVVVADATQRLQGAKPRIDMAWRLKVTRLPALIGFGV
                     AQAVESTMTAALRNSAAGRIAANVADNAWDFATFLAVPTILFEKAGPLAAVRRSGLLV
                     ASRWGTQLTAQAVLSFAIAFCTIPLAIVAIVVASANAALGIALLVGILLAAIAVSSAL
                     NGILSAAIYRFATTGMLAPGFNEADMWSVFAGR"
     gene            complement(765..1442)
                     /locus_tag="VII50_01910"
     CDS             complement(765..1442)
                     /locus_tag="VII50_01910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5049627.1"
                     /translation="MGPVILVPGYGGSDSMLETLANRLHSAGRTTILLVLPDDATGDL
                     NVQAGILAARVTQALNAGAPSVDLVGYSAGGIVIGLFVAAHPKDVRQVVTIGSPLHGT
                     EIAALAVTLNMSSSVCPVACREMAPGSSVLGSLDAAQPARTGVPWTSMWTTDDQVVLP
                     PDSARFTGATNIVLQQVCADDTANHLNLPADPLVAGLTLRALDTSDVGTPSASDCSSL
                     RAPATGA"
     gene            1468..1647
                     /locus_tag="VII50_01915"
     CDS             1468..1647
                     /locus_tag="VII50_01915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5049628.1"
                     /translation="MLADEASLGDATALTAGALLTGTGLDGNELGSSLGDARDVGGEV
                     TLEIGALALVAPDAE"
     gene            1787..2890
                     /gene="prfB"
                     /locus_tag="VII50_01920"
     CDS             1787..2890
                     /gene="prfB"
                     /locus_tag="VII50_01920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HEY5049629.1"
                     /translation="MPTADPREELKALDATLSSIEQVLDVPALRRQVAKLEEQAAVPD
                     LWNDPDEAQRVTSKLSHAQGDVRRVEGLRSRLDDVGVLLDLAESEADAGVHVEALAEI
                     IALTKAIGELEVRTLLSGEYDSREAVVTINSQAGGVDAADWAQMLLRMYLRWAERHNY
                     PTEVYDTSYAEEAGIKSATFAVKVPYAYGTLSPEHGTHRLVRISPFDNQGRRQTSFAG
                     VDVVPVVESSDHVEIPDDDIRVDVYRSSGPGGQGVNTTDSAVRITHLPTGIVVSCQNE
                     RSQIQNRASAMLVLQAKLLERRRQDEAAKMAEIRGDSTTSWGTQIRNYVLHPYQLVKD
                     LRTDVETGNTTAVLDGEIDEFIEAGIRWRRRAG"
BASE COUNT          487 a          949 c          997 g          462 t
ORIGIN      
        1 ctgggcagcg ttgacggcgg accccaccct gctgctcgtg ccgctggcgg ttctgttcgt
       61 cggaggcgtt gcatcggctg gctacattgc cgtattcggc ggcctcgccc atctgctcac
      121 cgggggtcgc ggcgccgtcg cggtcaagac gctgccacgt gccgtcgtcc tcgctgttgt
      181 ctctgtcatc ggccgcgcgg tcgtcgtcgc ggatgcaacg cagcgactgc aaggtgcgaa
      241 gccacgcatc gacatggcct ggcggttgaa ggtcaccagg ctgcccgcgc tgatcggctt
      301 cggtgtcgcg caagcagtcg agagcaccat gacggccgcg ttgcgcaaca gtgcggccgg
      361 acgcatcgct gcgaacgtgg ccgacaacgc gtgggacttt gcgaccttcc tcgccgtccc
      421 gacaatcttg ttcgagaagg ccggaccact cgcggcggtg cgccggtcgg gcttgctcgt
      481 ggcctcgcga tggggcacgc agctgactgc gcaagcggtg ttgtcgttcg caattgcatt
      541 ctgcacgatc ccgctcgcca tcgtcgcgat tgtcgtcgct tcagcgaatg ctgcgctcgg
      601 aatcgctttg cttgtaggga ttttgctcgc ggcgatcgcg gtgagttcgg cgctgaacgg
      661 catcttgtcg gcggcgatct accgcttcgc taccaccggc atgctggcgc ccggcttcaa
      721 cgaggcagac atgtggtcgg tgttcgcagg ccgctagacg gcagttaggc gccggtggct
      781 ggtgcgcgaa gactcgagca gtcggacgcc gagggggtgc ccacatccga cgtgtcgagt
      841 gcgcgcagcg tgaggcctgc tacgagtggg tcggccggca ggttgagatg gttcgcggtg
      901 tcatctgcgc acacctgctg aagcacgatg ttcgtcgcac cggtgaagcg tgccgaatcg
      961 ggtggcagca cgacttgatc gtcggtggtc cacatcgacg tccatggcac gcccgtgcgt
     1021 gcaggctggg cagcgtcaag cgacccgagc accgacgagc ccggtgccat ctcgcgacag
     1081 gcgaccggac ataccgacga cgacatgttc agcgtgaccg ccagcgcggc aatctcggtg
     1141 ccgtgtagtg gcgagccgat cgtgacgacc tggcgcacgt ccttcggatg cgctgccacg
     1201 aacaggccga tcacgatccc gccggccgag tagccgacga ggtcgacgga cggcgcgccg
     1261 gcattcaacg cctgggtcac tcgggcggcg aggatgcccg cttgcacgtt cagatcgccg
     1321 gtcgcgtcgt ccggcaagac gagcaggatc gtcgtccgcc cggcactgtg caatcggttc
     1381 gcgagggtct cgagcatcga gtcgctgccg ccatagccgg gcaccaaaat gaccggcccc
     1441 aaggctgaaa ggtcgagggg cgacggcgtg ctggctgatg aggcttcgct cggtgatgcc
     1501 acagcgctca cagcgggcgc gctactgact ggcaccgggc tcgatggcaa cgaactgggc
     1561 agctcgctcg gcgatgccag agacgtcggc ggcgaggtca cgctcgagat cggggcgctc
     1621 gcgctcgtcg caccggatgc tgagtgatgc gagccggagc acgccgacag cagcaagcac
     1681 gcggcacccg caagcgccgg ccagcgtcgg atcggcatac ctcattgtct cgctgccaca
     1741 gacgtggacg gcgggcgggc gcccgcagcg actacgctcg gacctcgtgc ctaccgccga
     1801 tccacgcgaa gagctcaagg ctctcgatgc cacgctgtcc agcatcgagc aagttctcga
     1861 cgtcccggca ttgcgtcgtc aggtggcgaa gctcgaggag caagcggccg tacccgacct
     1921 gtggaacgac ccggacgagg cgcagcgggt gacgagcaag ctctcgcacg cacagggcga
     1981 cgtgcgcagg gtcgaagggc tccgctcgcg gctcgacgac gtcggggtct tgctcgacct
     2041 cgccgaatcc gaagccgacg ccggtgtgca cgtcgaagcg ctcgccgaga tcatcgccct
     2101 gactaaggcc atcggcgaac tcgaggtccg caccctgttg tcgggggagt acgactcgcg
     2161 cgaggccgtc gtgaccatca actcccaagc gggcggcgtc gacgcggccg actgggcgca
     2221 aatgctgctg cggatgtacc tgcgctgggc cgagcggcac aactacccga ccgaggtcta
     2281 cgacacgtcc tacgccgaag aagccggcat caagtcggca accttcgcgg tgaaggtccc
     2341 gtacgcctac ggcaccctgt cgcccgagca cgggacgcat cgcctcgtgc gcatcagtcc
     2401 gttcgacaac cagggacgcc ggcagacctc gttcgccggc gtcgacgtcg tcccggtcgt
     2461 cgagtcctca gaccatgtgg agattccgga cgacgacatc agggtcgacg tctaccgctc
     2521 gtccgggccg ggtggccagg gcgtgaacac gacggactcc gcggtccgga ttacccacct
     2581 gcccaccggg atcgtggtga gctgccagaa cgagcgcagc cagatccaga accgggccag
     2641 cgcgatgctg gtgttgcagg ccaagctgct ggagcgacgg cgacaggacg aagcggccaa
     2701 gatggccgag atccgcggtg acagcacgac cagttggggc acccagatcc gcaactacgt
     2761 gctgcacccc tatcagctgg tcaaggacct gcggaccgac gtcgagacgg gcaacacgac
     2821 ggcggttctc gacggcgaga tcgacgaatt catcgaggcg ggcatccgct ggcggcggcg
     2881 ggcaggttag ctcat
//
LOCUS       DATCBJ010000091         2294 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_506146, whole genome shotgun sequence.
ACCESSION   DATCBJ010000091 DATCBJ010000000
VERSION     DATCBJ010000091.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2294)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2294)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2294
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_506146"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1176
                     /locus_tag="VII50_01925"
     CDS             <1..1176
                     /locus_tag="VII50_01925"
                     /inference="COORDINATES: protein motif:HMM:TIGR00711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HEY5049630.1"
                     /translation="GLALFTLASAACALAPNATDLIVARTIQGLGAAAVLPLSLTILT
                     TAFPPERRGLIVGIYGGLAGLAVATGPLVGGVVTEGLDWHWIFWINVPIGILAIALAA
                     RLLPESHGAAERVDLVGVALVTAGVVSLVWALVRANDVGWSSPEIVGTLVAGCVLLVA
                     FVGWERRVRAPMVPMRLFHSRTFAVGNVTMFLMSGATFAAAFLIIQEFQFARGYSPVS
                     TGLRLLPFFGTPMVVSPLAGALSDRIGRRPIMVVGLTLQALGFAWVAARGSLSTSWVE
                     LVLALLVAGIGISMALPTVPTAVLSCVDAVEMGKASGICAMSQRFGAVFAIAIGSAVF
                     SAHGNLSDPAAVTAGFRPALWSCVIFAALGAVTSAAITTRTSPVSRDTDVPDVLVNA"
     gene            1197..1646
                     /locus_tag="VII50_01930"
     CDS             1197..1646
                     /locus_tag="VII50_01930"
                     /inference="COORDINATES: protein motif:HMM:NF019928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HEY5049631.1"
                     /translation="MPDILHRISIDAPQKRVHDLIATTDGVARWWTGRPLDGESAVGG
                     SFGVYFGAAEKAAAVMQVMSATREEVVWRVIDGPDEWIDTLITFALRAASDGGTTLLF
                     SHSGWRAANEFMSGCSTNWGSYLTSLKTGAEGHEFGAFPAGEISRWS"
     gene            1706..>2294
                     /locus_tag="VII50_01935"
     CDS             1706..>2294
                     /locus_tag="VII50_01935"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HEY5049632.1"
                     /translation="MTLTDVSALAPVIVVSPHLDDAVLSCAGLIAGATGVTVVTVFAG
                     NPPARDAATPAEFLPGTTAWDQASGFVGGDDVVGLRRAEDRAALAHLGAVPQWLDFLD
                     SQYVVEPGESATPVHIAASIRDAIEELRPATIAFPLGLSHTDHVRTHEACFLLLQKSP
                     QLVQNWVAFIDVPYRALHRAQADVRLARLRELGYDL"
BASE COUNT          323 a          768 c          774 g          429 t
ORIGIN      
        1 ggcctggcgc tgttcacgct ggcctctgcc gcgtgcgcgc tcgccccgaa cgcgactgac
       61 ctgatcgttg cacggaccat tcagggcctg ggcgcggccg cggtcttgcc gctgagcctg
      121 acgatcctca ccacggcgtt cccaccggag cggcgcggat tgatcgtcgg catctacggt
      181 ggtctcgcgg ggctggctgt tgcgaccggg ccgctcgtgg gtggcgttgt caccgaggga
      241 ctcgactggc actggatctt ctggatcaac gttccgatcg ggatcctcgc gatcgcgctc
      301 gcggccaggc tgctgccgga gagtcacggt gccgcggagc gggtcgacct cgtcggcgtc
      361 gctctcgtga ccgcgggcgt cgtcagtctg gtctgggcct tggtgcgggc gaacgatgtc
      421 ggatggtcga gcccggagat cgtcggcacg ctggtggccg gctgcgtctt gctggtcgcg
      481 tttgtcgggt gggagcgccg cgtacgtgcg ccgatggtgc cgatgcggct gttccacagc
      541 cggacgttcg cggtcgggaa cgtcacgatg ttcctcatgt cgggggccac cttcgccgcc
      601 gcgttcctga tcatccagga gttccaattc gcgcgcggct actcgccggt gtcgaccggg
      661 ttgcggctcc tcccattctt cgggacgccg atggtcgtct caccgctcgc cggcgcgctc
      721 tcggatcgga tcgggcggcg tccgatcatg gtggtaggcc tgacgttgca ggcgcttggc
      781 ttcgcatggg tcgcagcacg agggtccctg agcaccagct gggtggagct cgtcctcgca
      841 ttgctcgtcg ccggcatcgg catctcgatg gcgctgccca ccgtgcccac cgccgtcctg
      901 tcctgcgtcg acgcagtcga gatgggcaag gcatccggaa tctgcgccat gtcgcagcgc
      961 ttcggtgccg tctttgcgat cgcgataggc agcgcagtct tttccgcgca cggcaacctc
     1021 agcgatccag ccgcggtcac tgccgggttc cggcctgcct tgtggtcgtg cgtgatcttc
     1081 gccgccctcg gcgcggtcac gtcggccgcc atcaccacac gcacgtcgcc ggtgagcagg
     1141 gacaccgacg tacccgacgt cctcgtcaac gcctaaccga tcgaaaggac acgacaatgc
     1201 ccgacatcct gcaccgcatc agcatcgacg ccccacagaa acgcgtccac gatttgatcg
     1261 ccaccaccga cggcgtcgcg cgctggtgga ccggccgtcc gctcgacggc gagagcgcag
     1321 tcggtggcag cttcggcgtc tacttcggtg ccgccgaaaa ggccgctgcc gtcatgcagg
     1381 tcatgtccgc cacacgggag gaggtcgtgt ggcgggtcat cgacgggccc gacgagtgga
     1441 tcgacaccct catcacgttc gccttgcgcg cagcgagcga tggagggacc acgctgttgt
     1501 tcagccactc cgggtggcgg gcggcgaacg agttcatgag cggctgcagc accaactggg
     1561 gctcctacct gacgagtctg aagaccggcg ccgagggtca cgagttcggt gcgttcccgg
     1621 ccggcgagat cagccgctgg agctgaactg tccggtctag accaccgggt gcgatggcag
     1681 cgatgaggag tgatacgaat ctcccatgac gctcaccgat gtcagcgctc tcgcgcccgt
     1741 catcgtggtt tccccgcacc tcgacgacgc ggtcctcagc tgtgccggat tgatcgcggg
     1801 tgctaccggc gtcacggtgg tcacggtgtt cgccgggaac ccgccggccc gtgacgcggc
     1861 gacgccggcc gagttccttc ccgggacgac ggcctgggac caggccagcg ggtttgttgg
     1921 aggcgacgac gtcgtgggcc tgcgccgggc ggaggatcgg gccgcgctcg cgcatcttgg
     1981 cgccgtaccg cagtggctcg acttcctcga cagccagtat gtggtcgagc cgggtgagtc
     2041 ggcgacacca gtacacatcg cggccagcat tcgcgacgcg atcgaagagc ttcgaccggc
     2101 cacgattgcg tttccgctag gcctttcaca caccgaccac gtgcggaccc acgaagcgtg
     2161 tttccttctc ctgcagaaat ctccccaact agtgcagaac tgggtcgcgt tcatcgacgt
     2221 cccgtatcga gctcttcatc gtgctcaggc ggatgttcga ctcgctcgcc tgcgtgagtt
     2281 gggatatgac ctcg
//
LOCUS       DATCBJ010000092         3484 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_358453, whole genome shotgun sequence.
ACCESSION   DATCBJ010000092 DATCBJ010000000
VERSION     DATCBJ010000092.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 3484)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3484)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3484
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_358453"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..769)
                     /locus_tag="VII50_01940"
     CDS             complement(<1..769)
                     /locus_tag="VII50_01940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5049633.1"
                     /translation="MGILTAVCADKGSPGATTLAVLLAAAHPNQPILVEADPAGGDLT
                     HRLFAGTGMTPPPGANLLTLAGESRRGAPAAMLAAHAAITPVGVRLVEGLSSDEQQVG
                     LAPLWRGLAAALVASDDDVIADLGRVSTTHPGLVVVASADRIVIVVRPDQEQLVRLPG
                     RIRHLLSVAAGDVSKVLVVVVSPEKAARRDQRGVAAVLAAEGLSAVPVVWFPFAPKEV
                     AAFYAGRPNRRGYLWRAAEGLAAELRVPTEEHPEASAA"
     gene            complement(776..1507)
                     /locus_tag="VII50_01945"
     CDS             complement(776..1507)
                     /locus_tag="VII50_01945"
                     /inference="COORDINATES: protein motif:HMM:NF020250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAF domain-containing protein"
                     /protein_id="HEY5049634.1"
                     /translation="MTATHTSFPPPQQSAPHIPPHQVSGRLPTRQRRPALIAVAVLLI
                     VSGALGGLQLLTASSHTVSVLVLVRPVPAGHVIESVDLGTTALSGKVAYTPGSAEANV
                     VGKTAARDLFAGQLLTQSMMATASVPDGTQALVGLRLSAAQVPSAGLADGNTVVLVEV
                     PSANGAAAVAAPLASGQATTPSSATSGVATPNLVLATGTVYAIAADTASAGGALITVL
                     VPREQSEALSVAASSGQVTLIRVGS"
     gene            complement(1533..2237)
                     /locus_tag="VII50_01950"
     CDS             complement(1533..2237)
                     /locus_tag="VII50_01950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5049635.1"
                     /translation="MDCNGCIWSPWSAGPGLQDDPAYLQWLAAGGKGSLSQEACYPGP
                     TTTVVYFPPGAAPPPPGLPASQLGAAAVAMLPLPAMVTASAPGAIGRPFAQTFVNFPT
                     FLWLNSAQWHDYTATANDGFKAVTAIAVPSSVTWTMGDGHATTCGGPGVAWQPGVDQS
                     DCTYTYATSSVHQAQVGSDVNDRPYVVVAAVTYQVTWTCAGACGGVAAGSVGPVTGPG
                     SPMPLVVGEIQTVVTG"
     gene            complement(2489..3175)
                     /locus_tag="VII50_01955"
     CDS             complement(2489..3175)
                     /locus_tag="VII50_01955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5049636.1"
                     /translation="MTERPLRRLPSGHRALRVFLLVCVGVAAVVGVVFAVTLLPEHHG
                     SAPKPATAASAAASQSLSPSLDVAPSSATSPTAVSAPASEPALSASVAQPDPQVALKA
                     AVVAAQSGFEATLNQELDSNTGNLTPLFAVATGQALANAEDAVAKLRSQGRTQRGAPT
                     ITDATVVSVASPMVAVVSACEDDSTARIVDAKTGALIDQLHERYTTTTTMVLLGGSWK
                     ASTSSSGGAC"
BASE COUNT          608 a         1195 c         1137 g          544 t
ORIGIN      
        1 ccgcagcaga cgcttcaggg tgctcctcgg tcggcacgcg aagctcggcc gcaaggcctt
       61 cggcggcccg ccacaggtag ccgcgccgat tcgggcggcc tgcgtagaac gcggcaactt
      121 ccttgggggc aaagggaaac cacacgacgg gaacagccga gaggccctcc gcggccaaca
      181 ccgcagcgac ccctcgttgg tcgcggcgag ccgctttctc cggcgagacg accacaacga
      241 gcaccttgga aacgtcgccc gcggccacgc tcagcaggtg acgaatgcgc ccagggagcc
      301 gcaccagctg ttcctggtca gggcggacca cgatcacgat ccggtccgcc gaggccacca
      361 cgacaagccc aggatgtgtt gtcgagactc ggcccaggtc agcgatcacg tcgtcgtcac
      421 tggcgaccag ggcggccgcg aggcccctcc acagaggcgc caggccgact tgctgttcgt
      481 cggagctcag gccctccacc agccgcacgc cgaccggcgt gatcgcggcg tgcgcagcga
      541 gcatcgctgc aggcgcaccc cgacgtgact ccccggcaag tgtcagcaag ttcgcccccg
      601 gcggtggcgt cataccggtg cccgcgaaca gccggtgggt caggtcaccg cctgccggat
      661 ccgcttcgac gaggatcggc tggttcgggt gcgcggctgc cagcagcacg gcgagcgtgg
      721 tcgcaccggg actgcccttg tcggcgcaca ctgcggtcaa gatgcccaac gctggtcagc
      781 tcccgaccct gatcaatgtg acctggccgg agctggcagc gaccgacaac gcctcggact
      841 gctcccgcgg cacgagcacc gtgatcaatg ccccgccggc cgatgccgtg tcggccgcga
      901 tcgcgtacac cgtgccggtg gccagcacca gattcggagt cgccacacca ctcgtggcgc
      961 tcgacggtgt ggtcgcttgc ccgctcgcca gcggcgccgc gaccgcagcg gccccgttgg
     1021 ccgagggcac ctcgaccagc acgactgtgt tgccgtcggc aagccctgcc gatggcacct
     1081 gggcagcgct caaacgcaat cccaccagcg cctgcgttcc gtcgggcact gatgcggtcg
     1141 ccatcatcga ctgcgtcagc agctgccccg cgaacaggtc gcgtgcggcc gtcttgccga
     1201 cgacgttggc ttctgccgag cccggtgtgt aggcaacctt gcccgacagt gccgtcgttc
     1261 ccagatcaac cgattcgatg acgtggccag ccggaacggg cctgactagc acgagtacgg
     1321 acacggtgtg gctggatgcg gtcaacagct gaaggccgcc gagtgcgccg gatacgatca
     1381 acaacacggc gaccgcgatc agcgcagggc ggcgctgccg ggtcggcaga cgtccgctga
     1441 cctgatgtgg cggaatgtgt ggcgcactct gctgtggtgg cggaaaactt gtgtgggtcg
     1501 cggtcatgcg aacctcctcg agcgtgcggg cattagccgg tcaccacggt ctggatttcg
     1561 ccgacgacca gcggcatcgg cgagccaggg ccggtcaccg ggccgacgct gccggcggcg
     1621 actccgccgc atgcgcccgc gcacgtccac gtcacctgat aggtgacagc cgcgacgacg
     1681 acgtacggcc ggtcgttgac atcactgccc acctgcgctt ggtgcaccga cgacgtggca
     1741 taggtgtagg tgcagtcgga ctgatcgacg ccgggctgcc atgcgacccc tgggccgccg
     1801 cacgttgtgg catgaccatc gcccatcgtc caggtcaccg acgacggcac cgcgattgcc
     1861 gtcactgctt tgaaaccgtc gttggccgtc gccgtgtagt cgtgccactg cgcactgttc
     1921 agccaaagaa aggtcgggaa gttcacgaac gtttgcgcga acggccgacc gatggcgcct
     1981 ggtgcagaag ctgtcaccat cgccggcaac ggcaacatcg cgaccgccgc agcgcctaac
     2041 tgcgacgccg gtaggccggg cggaggaggt gccgcaccgg gcggaaagta gacgacagtt
     2101 gttgtcggcc ccgggtagca cgcctcctgc gacagactgc ccttcccccc cgccgcaagc
     2161 cactgcagat acgcggggtc atcttgaagc ccagggcccg ccgaccacgg gctccagatg
     2221 catccgttgc aatccacgcc cgcaggaatc cctggcccac tcggcggaac gcacccggcg
     2281 ccagaacttg caaccggctg ccctcccacg ccagcgcctg gaccgccagg gtcggcgacg
     2341 tgcacgacgc agtgcgggtt gttcggatcg gtgcagacaa cagggacggc cgcgtttgcg
     2401 atccctgcag cgaccaccaa ggcaccgctg caggctgcca acgggatgac aaaagcgatt
     2461 agcatttgtg caatacgtcg aggccacatc aacaggcacc gccggaggaa ctggtggatg
     2521 ccttccacga gccacccaag agcaccatcg tcgtggtggt tgtgtaccgc tcgtggagtt
     2581 ggtcgatgag ggcgccggtt ttcgcgtcga cgattcgagc ggtcgagtcg tcttcgcatg
     2641 cagatacgac tgccaccatt ggggacgcga ctgacaccac cgtggcgtca gtaatcgtcg
     2701 gtgcgccacg ctgcgttcgg ccctggctgc gcagcttggc cacggcgtct tcggcgttgg
     2761 cgagcgcctg accggttgcg acggcaaaca gcggagtgag gtttcccgtg ttcgagtcga
     2821 gttcttggtt gagagtggct tcgaaacccg actgggcagc gaccaccgcg gcttttaggg
     2881 ccacctgagg atcgggctgt gccaccgacg cgctcagcgc cggttcactc gctggtgccg
     2941 agaccgcggt tggcgaggtc gcgctgctcg gcgcgacgtc aagcgacggc gacagcgatt
     3001 gcgatgcggc ggccgacgct gcggtggcgg gcttcggcgc actcccgtgg tgctcgggga
     3061 gcaacgtgac cgcgaaaacg acgcccacaa cagccgcgac acctacgcac accagcagga
     3121 atacgcgcaa cgcgcggtgc cctgacggta agcgacgcag cggacgctca gtcattgtcg
     3181 gacctccccc gtggcggtcg tgacgattca caccgctcag gcggagtcgt acgccgacca
     3241 ccgtagagtg cgaaacccga cgtcggcacc ttccaatcgg atgagtgacg cgacgtgcat
     3301 atctggtaca cacaccgtgt atagacttct cgcccattgc gccgcatatc ggcggccagc
     3361 agtagcgcag cccacaacat tggtcgctgc cacgttggtc gcgacctcct acaactggat
     3421 gtcttgagca ccatcaaacg tctccttcaa gtcgtattcc tcgaatgagt tgtccctctt
     3481 gtag
//
LOCUS       DATCBJ010000093         2116 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_475777, whole genome shotgun sequence.
ACCESSION   DATCBJ010000093 DATCBJ010000000
VERSION     DATCBJ010000093.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2116)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2116)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2116
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_475777"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(164..>2116)
                     /locus_tag="VII50_01960"
     CDS             complement(164..>2116)
                     /locus_tag="VII50_01960"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009081111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HEY5049637.1"
                     /translation="PLTGRRIPIVADEHVDPAFGTGAVKVTPAHDPNDFEIGQRHGLP
                     AITVLDERGVITAHGPFKGLDRFEARPAVTAALRELGLVVAEKRPYIHSVGHCSRCDT
                     VVEPRLSLQWFVKVESLAKAAGAAVRDGRVTIHPPEMARNYFDWVDNMHDWCISRQLW
                     WGHRIPVWYGPDGEVVAVGPDDEPPSGDGWRQDEDVLDTWFSSALWPFSTLGWPAKTA
                     DLEKFYPTSVLLTGYDIIFFWVARMMMFGLYAMADRGPEGSVPFRTVALTGLVRDDRG
                     RKMSKSAGNGIDPLEWIDTYGADAARFTLARSASPGSDIPVSEELAQGGRNFITKLWN
                     ATRFAILNGATVEGPLPDLETLPATERWILSRLVRTAADVDGYFDDFQFAKACDALYH
                     FTWDELCDWYLELAKLPLERGGVEATATRRVLGHVLDVLLRLWHPVIPFVTEELWTTL
                     TARETVVLAGWPTGLVTGGTTALVDGATVDGPAEALITQLQAVVTEVRRFRAEQGLRP
                     AQRVPAHIDGLTNGALAPFEDQIRSLARLQIPTDGFRETAAVVVGHSERAVRVALDLS
                     GTIDIGKERARLTKAFDVAVNEIALAEAKLADDAFIAKAPAPVVDKIRARLRDAQAEV
                     DRLRGQLDALPLEVLPPDAPPPQSPQ"
BASE COUNT          377 a          703 c          684 g          352 t
ORIGIN      
        1 ccggcgatgt gcaccgacgg atacgcgaga tgcggatcac ccatgagatc catcagcgcg
       61 gtcatgcgcc cgggcttcca ctcgatgcgc gactcgggcc agcgcgcgag caactcgctc
      121 tcaacgcgtt cgagttcgag cgcctgctca ctcatcggcg caatcattgc ggcgactggg
      181 gaggcggtgc atcaggaggc agtacctcaa gtggaagggc gtcgagctgt ccccgcagcc
      241 gatcgacttc ggcctgggcg tcgcgaagtc gggcgcggat cttgtcgacc accggggccg
      301 gcgccttcgc gatgaatgcg tcgtcggcta gcttcgcctc cgcgagcgcg atctcgttca
      361 ccgcgacgtc gaaggctttg gtgagacggg cgcgctcctt gccgatgtcg atcgtgcctg
      421 acagatcgag agcaacccgc accgcgcgct cgctgtggcc aaccaccacg gcggccgttt
      481 cacggaagcc gtcggttggt atctgcaatc gcgcaagaga ccggatctgg tcctcaaacg
      541 gcgcgagcgc accgttggtg aggccgtcaa tgtgtgccgg aactcgttgc gccggccgaa
      601 ggccctgctc ggcgcggaac cgccgaacct cagtgacgac cgcctgcagt tgcgtgatga
      661 gtgcctcggc cggcccatcg accgtcgcgc catcgaccaa ggcagttgtg ccgccggtca
      721 cgagaccggt cggccaccct gcgagcacga cggtctcgcg ggcggtcaag gtggtccaca
      781 attcctctgt cacgaagggg atcactggat gccacagtcg tagcagcacg tcgagaacgt
      841 ggcccagcac gcggcgggtg gccgtcgcct cgacgccgcc cctttcgaga ggcaacttcg
      901 ccaactcgag gtaccagtcg caaagctcat cccaggtgaa gtggtagagc gcgtcgcacg
      961 ccttggcgaa ctggaagtcg tcgaagtacc cgtcgacgtc agctgcggtg cggaccaacc
     1021 tcgacaggat ccaacgttca gtcgccggca acgtctcgag atcgggcaac ggaccctcga
     1081 ccgtggcgcc gttgagaatc gcgaaccgtg ttgcgttcca gagcttggta atgaagttgc
     1141 ggccgccttg cgccagctct tcactgaccg ggatgtcgga ccccggtgag gcgctgcggg
     1201 caagggtgaa acgcgctgcg tcggcgccgt aggtgtcgat ccattcgagt ggatcgatgc
     1261 cgttgcccgc cgacttgctc atcttgcggc cgcggtcgtc gcgcaccagg ccagtgagcg
     1321 caaccgtacg gaacgggacc gagccttcgg ggccccggtc ggccatcgca tacaggccga
     1381 acatcatcat tcgcgcaacc cagaagaaga tgatgtcgta gcccgtgagt aacaccgacg
     1441 tcggatagaa cttctcgagg tcggctgtct tggcgggcca cccgagggtc gaaaagggcc
     1501 acaacgccga agaaaaccag gtatcgagga cgtcctcgtc ctggcgccaa ccgtcgccgc
     1561 tcggcggctc gtcgtccgga ccgactgcaa ccacttcgcc atccggcccg taccacaccg
     1621 gaatccggtg accccaccac agctgccgcg agatgcacca gtcatgcatg ttgtcgaccc
     1681 agtcgaagta gttgcgcgcc atctcgggcg gatgaatcgt cacacgacca tcgcgaactg
     1741 cggcacccgc cgccttcgcc agcgactcga ccttgacgaa ccattgcagc gacaacctcg
     1801 gttcgaccac cgtgtcgcat cgagagcaat ggccaacgga gtgaatgtag ggtcgcttct
     1861 cggcgacgac caggcccagc tcgcgtagcg ctgcggtgac cgcgggcctt gcctcgaatc
     1921 tgtcgagacc cttgaagggg ccgtgtgcgg tgatcacgcc gcgctcgtcg agcaccgtga
     1981 tcgccggcaa tccgtggcgc tggccgatct cgaagtcgtt cggatcgtgc gccggcgtca
     2041 ccttcaccgc gccggtgccg aacgcgggat cgacatgttc gtccgcgacg atcgggatgc
     2101 gccggccggt cagtgg
//
LOCUS       DATCBJ010000094         2292 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_395312, whole genome shotgun sequence.
ACCESSION   DATCBJ010000094 DATCBJ010000000
VERSION     DATCBJ010000094.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2292)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2292)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2292
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_395312"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..212
                     /locus_tag="VII50_01965"
     CDS             <1..212
                     /locus_tag="VII50_01965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="macrolide ABC transporter permease"
                     /protein_id="HEY5049638.1"
                     /translation="LSFIGGVLGVGAGLLAGFLVPRLAGIAVTITATPVIISVCVAAG
                     VGLIFGVYPAARAARLAPIAALRAE"
     gene            250..1203
                     /locus_tag="VII50_01970"
     CDS             250..1203
                     /locus_tag="VII50_01970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5049639.1"
                     /translation="MNRAILGAAGVTACLGIAACGGAAASGGASPTATPSAGGRAAFA
                     RNAAFGQLVQINGSTLILSGSTGDSTVTYASTTTITQTSTGTLADITPGTCITATGTK
                     DAAGTLTATSVSLAAAVSGSCVTAGFGGGTFSPRPGATPRPSFSPPAGAAGFTAARGQ
                     VKSVTGTSVTLTETTGTVTTTTIINVPTTVKVTTSTAIKATALQTGECVTAVRPKASS
                     GTVAASSLIISPAAPMAALKASVALAAVGLAVQAPAPRVRNGISMRVLVAEDHRGLRE
                     VLVQGQSRDTRVVVVVLRWNAQIWATSSPKSKANALAPATH"
     gene            complement(1348..1623)
                     /locus_tag="VII50_01975"
     CDS             complement(1348..1623)
                     /locus_tag="VII50_01975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5049640.1"
                     /translation="MDGDQFIPSPPTPPPEQPERQATGFRKVAATALLSVGLLAGSGI
                     GGYAIVHAAGSSNTAASSTAATTTAATPTPAATPTCPNRGTSTGTTG"
BASE COUNT          353 a          768 c          786 g          385 t
ORIGIN      
        1 cgctgagctt catcggaggc gtgctcggtg tcggagcggg tctgctcgcc ggattcctgg
       61 tgccgcgcct cgccggcatc gcggtgacca tcacggcgac tccggtgatc atctccgttt
      121 gcgttgcggc cggagttggc ctgatcttcg gcgtctaccc cgcggcgcga gcggctcgcc
      181 tcgcgccgat tgcggcactg cgggctgaat gacctcacac gctgtcatcc gtttgggaga
      241 acgaacctca tgaaccgtgc catcctcggc gccgccggcg tcacggcctg cctcggcatc
      301 gccgcctgcg ggggcgcagc ggccagtgga ggtgcaagcc ccaccgcgac gccgtcagct
      361 ggcggtcgcg cggcgttcgc gcgcaacgcg gccttcggtc agctggtcca gatcaacgga
      421 tcaacgctga tcctgagcgg cagcaccggc gactcgacgg tgacatacgc ctcgacgacc
      481 accatcacgc agacgagcac gggcaccctc gcggatatca cgccgggcac ctgcatcacc
      541 gccacgggca ccaaggacgc ggctgggacg ctgaccgcga cgtcagtgag cctcgccgcc
      601 gcagtgagcg gttcttgcgt caccgccggg ttcggcggcg gcacgttcag cccgcggccg
      661 ggggccaccc cgcgcccgag cttctcgcct ccggctggtg ccgccgggtt caccgccgcg
      721 cgcggccagg tcaaatcggt gaccggcaca tcggtcacgc tcaccgagac gacagggacg
      781 gtgaccacca ccacgatcat caatgtcccg acgaccgtca aggtgacgac ctcgaccgct
      841 atcaaggcga ccgcgctcca gaccggtgag tgcgtcaccg cggtccgccc caaggccagc
      901 tccggcacgg tcgcggctag ctcactgatc atctctcctg cggcgccaat ggctgcactg
      961 aaggcttcgg tggctttggc ggcggtgggt ttggccgtgc aggcgccggc accacgggtg
     1021 cgtaacggga tcagcatgcg agtcctggtt gcggaggacc accgcgggct ccgggaggtc
     1081 ctcgtccagg gtcagtcccg ggatactcgt gtcgtcgttg tggtgctccg ctggaacgcg
     1141 cagatctggg cgacgagctc gccgaagtcg aaggcgaacg cgcttgctcc ggctactcac
     1201 taggtggacg tagatgcatt cggtcacact gtcgcggaac ctgctcccga cccgacctac
     1261 ccgcgcctcg atgactggga tggcaggctg ttcgagcagt ggtgagacgg cccgccacat
     1321 tggcgggccg tctgcaggac tgtccggcta gcccgtggtg cccgtcgagg tgcccctatt
     1381 cgggcacgtc ggtgtagcgg ccggcgtcgg ggttgccgcg gtcgtggttg cggcggtgct
     1441 cgacgccgcg gtgttcgagc tgccggctgc gtgaacgatg gcgtagccac cgattcctga
     1501 tccggcgagc aggcctacgc tgagcagagc tgtcgccgcg accttgcgga aacccgttgc
     1561 ctgccgctcc ggctgctccg gcggcggggt cgggggcgat gggatgaact ggtcgccgtc
     1621 catggtgtgt acctcctagg tggactgacg tttacgtcga ccagcgtcgc gctcgggcgt
     1681 gggaacccgg tgggcgcctc cttggagacc gcttggagtt tgtgactacg tgtcggcgtg
     1741 atcctcgtgg tcggggcgtc ggtagttcct tcacgccgcc gtaaggcaac ccgacgctgc
     1801 ctgcatagcg tcgcggctca tggacatgct cggcgcgagg ggcatccatg gggccttgac
     1861 gcacggaccc ttccgaccag gccatcagga ggcgatggtc taccaaggtg atgatgacca
     1921 ccggaaggaa tggtcagggg gctgactcgt cctactagag ccactacgtt gaaccgagcg
     1981 ggtgcctcga tcggtcatgg cccctcggca caatctcatg ggccgctgtc ggagtgccgc
     2041 cgattgcccg ccaaacgagc gccgccgagc gctgtggcca attccgtggc ggaggtgtgc
     2101 ccgcgagggc gcgggcgtac cacgatcccg tacacatggt gaccgccaca tcgagatcgg
     2161 cgtcagtgtc gatcaaagcg agccgctgcg ctcgctcgag aataagccga agtcgacgac
     2221 gccgaggtgc gaccacctgg gcgactgcca gcgaacccgt gagcttcgaa aaacacatca
     2281 aaacgatgtt cc
//
LOCUS       DATCBJ010000095         4615 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_322134, whole genome shotgun sequence.
ACCESSION   DATCBJ010000095 DATCBJ010000000
VERSION     DATCBJ010000095.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 4615)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4615)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4615
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_322134"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..307)
                     /locus_tag="VII50_01980"
     CDS             complement(<1..307)
                     /locus_tag="VII50_01980"
                     /inference="COORDINATES: protein motif:HMM:NF013188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HEY5049641.1"
                     /translation="MHDVTSFGGVVLVWGLVGTAALLARRVARWVGIPAPAVFLLAAA
                     VAANSIKALRTAVDLHVVENVVTLALIVVLFDGGLHLGRRRFREAAVPIVALGVLGTF
                     "
     gene            complement(421..2397)
                     /gene="acs"
                     /locus_tag="VII50_01985"
     CDS             complement(421..2397)
                     /gene="acs"
                     /locus_tag="VII50_01985"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020664180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003987 - acetate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019427 - acetyl-CoA biosynthetic process
                     from acetate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HEY5049642.1"
                     /translation="MTGAVPEANLSNLLHETRRFDPPPKLAADAIAKKEVYAEAAKDP
                     LAFWEKQANRLTWAQPWAQVLDWSDAPFSKWFVGGKLNVAVNCLDRHVEAGHGDQVAY
                     HWEGEPGDTIAITYADLTKRVCQAANALTELGVAAGDRVMIYMPMIPETVVSMLACAR
                     IGAPHSVVFGGFSAEALAGRTNDCEAKFIITADGGYRRGAASALKPAVDLAIKECPQV
                     ETVLVVRRTGQDVEWYEGRDVWWHDVVDKQPETHVAQAFDAEQPLYIMYTSGTTAKPK
                     GILHTSGGYLTGVSATHKMVFDLHEDTDTYWCAADIGWVTGHSYIVYGPLANRATSVM
                     YEGTPDTPARDRWWSMIAKYKVSILYTAPTTIRTFMKWGEEFPAAHDLSSLRLLGSVG
                     EPINPEAWMWYRKNIGSDSCPIVDTWWQTETGSILITPLPGVTSTKPGAAMTAFPGIA
                     ADIVDNDGKSVGNGEGGYMVISQPWPSMLRGIWRDPERYKETYWSRFPGLYFAGDGAK
                     KDEDGDFWLLGRIDDVMNISGHRISTTEVEHALVGHPKVAEAAVVGANDPITGQAIVA
                     FVTVKGNVPDSESGGAAFAAELRAHVAKEIGAIAKPRDILISPDLPKTRSGKIMRRLL
                     RDVADHRALGDTTTLLDPNVVASIAAQMPASTEE"
     gene            complement(2750..3580)
                     /locus_tag="VII50_01990"
     CDS             complement(2750..3580)
                     /locus_tag="VII50_01990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009084385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IB family hydrolase"
                     /protein_id="HEY5049643.1"
                     /translation="MSTTGRPAAFFDLDKTIIAKSSVMAFGRPFYQGGLINRRALLKS
                     TYAQFVYLVGGADHDQIERMRAYLSQLCKGWDVEQVKAIVAETLHDLIDPLIYDEAAA
                     LIEEHHAAGLDVVIVSTSGIEVVEPIGELLGADRIIATRMVVEDGRFTGDIDYYAFGE
                     AKAAAVIELAAKEGYDLAASFAYSDSSTDIPLLEAVGFPSVVNPDRALRKEAVSRGWP
                     VLNFDNPVPLRARVGLGSPRSPVYAAAGLGAGMAAAGLVWYARRRSKDGQPARAAAKT
                     "
     gene            3905..>4615
                     /gene="ssd"
                     /locus_tag="VII50_01995"
     CDS             3905..>4615
                     /gene="ssd"
                     /locus_tag="VII50_01995"
                     /inference="COORDINATES: protein motif:HMM:TIGR03815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein Ssd"
                     /protein_id="HEY5049644.1"
                     /translation="MPISPDSVARHRPLLVTADPDLLDDILRLGAAAGVDVDVAHDPV
                     GARLGWSNAALVLVGDDVSNAVGRATLRRRAGVVLVGRDLGDGDIWKRAVGVGAQHVA
                     LLPDAESWLIARLADAVDEGAARALTVAVIGGRGGAGASTLAAALAVTAMRGGRRVML
                     VDADPYGGGADLLFGGAHAAGLRWCDIAGASGRIGGEELHAALPHIGSLAVLSYDRGD
                     PLDLRADAMSSVLAAAARV"
BASE COUNT          692 a         1535 c         1562 g          826 t
ORIGIN      
        1 cgaacgtccc gagcacgccg agggcgacga ttgggaccgc ggcctcgcgg aaccgccgtc
       61 gtccgagatg cagtccgccg tcgaaaagga ccacgatcag ggctaacgtg accacgttct
      121 cgactacgtg caggtcgacc gcggttcgca gcgccttgat cgagttggca gctaccgcgg
      181 cggcgagcag aaagaccgcc ggggccggga tcccgaccca gcgcgcgacg cgacgtgcga
      241 gcagtgcggc ggtgccgacg aggccccaga cgagcacgac gccgccgaac gacgtgacgt
      301 cgtgcacggg agcacctcac gggagtcggc gggcaggggt gaaccatgcc gaccagactt
      361 cccggcgcac cacgccgcag cctatcccgt cgtccggccc gcggcgggtg gctgtcgggg
      421 ttactcctcc gtcgaggctg gcatttgcgc ggcgatgctg gcgaccacgt tggggtcaag
      481 cagcgtggtc gtgtcaccca gggcacggtg gtcggccacg tcgcgcaaca accggcgcat
      541 gatcttgccc gaccgcgtct tcggtaggtc gggactgatc aggatgtccc gcggtttcgc
      601 gatcgcaccg atctctttgg cgacgtgcgc gcggagctcg gctgcgaacg ccgcgccccc
      661 ggattcgcta tcgggcacgt tgcctttcac cgtgacgaag gcgacgatcg cctggccggt
      721 gatcggatcg ttggcgccga cgaccgctgc ctcggcaacc ttcggatgac cgacgagcgc
      781 gtgctcgacc tcggtcgtcg agatgcgatg accggagatg ttcatgacgt cgtcgatgcg
      841 gccgagcagc cagaagtcgc cgtcctcgtc cttcttcgcg ccgtcgccgg cgaagtacag
      901 gccggggaac cgtgaccagt aggtctcctt gtagcgctcc gggtcgcgcc agattccgcg
      961 cagcatcgat ggccacggct ggctgatgac catgtagccg ccttcgccgt tgccgaccga
     1021 cttcccgtcg ttgtcgacga tgtcggcggc gatgccaggg aatgcggtca tggccgcgcc
     1081 cggcttggtc gaggtgacgc ccggcaacgg agtgatgagg atcgacccgg tctcggtctg
     1141 ccaccacgtg tcgacgatcg ggcagctgtc gctgccgatg ttcttgcggt accacatcca
     1201 cgcttcgggg ttgatcggct ccccgacgct acccagcagc cgtagcgacg acaggtcatg
     1261 tgcagcaggg aattcctcgc cccacttcat gaaggtgcgg atcgtggtgg gggcggtgta
     1321 gaggatcgag accttgtact tggcgatcat cgaccaccag cggtcgcgcg ccggggtgtc
     1381 gggcgtgcct tcgtacatga cggatgtcgc acggttggcg agtgggccat agacgatgta
     1441 actgtggccg gtgacccagc caatgtcggc ggcgcaccag taggtatcgg tgtcctcgtg
     1501 caaatcgaag accatcttgt gggtggcgct gacaccggtc aggtagccgc cgctggtgtg
     1561 cagaattccc ttcggcttcg ctgtcgtgcc gctcgtgtac atgatgtaga gcggctgctc
     1621 ggcgtcgaat gcctgtgcta catgggtttc cggctgcttg tcgacgacgt cgtgccacca
     1681 cacgtcgcgg ccctcgtacc actcgacgtc ctgtccagtg cggcgcacaa ccaggacggt
     1741 ctcgacctgc gggcactcct tgatcgccaa gtcgacggcg ggcttcaacg cgctcgcggc
     1801 gccacgtcgg taaccgccgt ccgcggtgat gatgaacttc gcctcgcagt cattcgtgcg
     1861 gccggcaagt gcctcggcgg agaagccgcc gaacaccacc gagtgcggcg caccgatacg
     1921 cgcacacgcc agcatcgaga cgacggtctc gggaatcatc ggcatgtaga tcatcacccg
     1981 gtcgccagct gcgacgccga gttccgtcaa cgcgttcgcg gcctgacaca cccgctttgt
     2041 gaggtcggcg tacgtgatgg cgatggtgtc gcccggctcg ccttcccagt ggtaggcgac
     2101 ctggtcgcca tgaccggctt cgacgtgacg gtcgaggcag ttcaccgcga cgttgagctt
     2161 gccgccgacg aaccacttgg agaacggcgc gtcactccag tccagaactt gcgcccacgg
     2221 ctgcgcccac gtcagccggt tcgcctgctt ttcccagaag gccaaaggat ctttcgcagc
     2281 ctctgcgtag acctccttct tcgcgatcgc atccgcggcc agcttcggcg gcggatcgaa
     2341 ccgcctggtc tcgtgcaaca agttcgacag gttggcctcg ggaactgctc cggtcacgat
     2401 gcgccttctt tcgagtgatg ctgtggctgc gtagcgaggt tctgctggac tgcgcagtga
     2461 gaactcatag tgcgagcggc gccccgttca cacgggacgc cgccgctcgc acgatcctcc
     2521 ggttaccaag cgtgcacctg cgcattgtcg gcgagcaggc aactccgagg agctgccgcc
     2581 cgactcgacc gcctgattcg gctggttccg cgtgggtgcc tgaggtgtcg tgcgcgagtc
     2641 cggaacctcg agggagaccc ctcgcggtac ccgacccatg cctagtttct acgcctaacg
     2701 ccacccgatc ggaagagtct tgcgccttgg aagacggtca gctttctcct caggtctttg
     2761 ctgcggccct cgcgggctgg ccgtccttgc tgcggcggcg ggcgtaccag accagcccgg
     2821 cggcagccat gcctgcaccc agtccggcgg ccgcgtaaac cgggctgcgc ggcgacccga
     2881 ggccgactcg cgcccgcagt ggcaccgggt tgtcgaagtt caacaccggc cagccgcggc
     2941 tgaccgcctc cttgcgcaac gcgcgatcgg gattgacgac cgacgggaag cccaccgcct
     3001 ccaggagcgg gatgtcggtc gacgaatcgc tgtaggcaaa ggaagctgca aggtcgtagc
     3061 cctctttggc cgcgagttcg atgaccgctg ccgccttcgc ctcgccgaac gcgtaatagt
     3121 cgatgtcgcc ggtgaagcgc ccgtcttcga ccaccatccg ggtggcgatg atccgatccg
     3181 cgccgagcag ttccccgatc ggctcgacta cttcgatgcc cgacgtgctc acgatcacga
     3241 cgtccaggcc cgccgcatgg tgctcttcga tcaaggcggc agcctcgtcg tagatcagcg
     3301 gatcgatgag atcgtgcagc gtctccgcga cgatcgcctt gacctgctcg acgtcccatc
     3361 ccttgcacag ttgagagaga taggcgcgca tgcgttcgat ctggtcgtgg tcggccccac
     3421 ccaccagata gacgaactgg gcgtaggtgc tcttgagcag cgcccgccgg ttgatcaggc
     3481 cgccttggta gaagggtcgc ccgaaggcca tgacactcga cttggcgatg atcgtcttgt
     3541 cgaggtcgaa gaatgccgcc ggccggccag tggtgctcat gctctgagga taagacgccg
     3601 gacctgatca tccctgcgtc ggcgtgggcc ttttgtcaca acctgttctt gtcacgaatt
     3661 gattgcgaca gggggatgtg cgagacgggg cccttgcctt acgttagtcc cggtacggct
     3721 ctccccccag agtcggaccc ttcgtgaacg gcccccgcta cccccccggc gggggccgtt
     3781 ccatgtcccg ggaccgattg cgtcggggtc cgtgggttgt ccccagcggc gcgtcggtgg
     3841 gtgccgcgtc cacagatcca ggtcggccac gcgatcgccc tgtgccggcg ttgatcgttg
     3901 gctcatgccg atctcgccgg attccgtcgc ccgccaccga ccgctgctcg tcaccgcgga
     3961 tccggacctg ctcgacgaca tcctgcggct cggtgccgcc gccggggtcg acgtcgacgt
     4021 cgcgcacgac ccggtgggcg cgcggctggg gtggtcgaac gcggcgctgg tgctggtcgg
     4081 cgacgacgtg tcgaacgcgg tcggccgtgc aacgctgcgc cgccgagcgg gcgtggtgtt
     4141 ggtgggtcga gatcttggcg acggcgacat ctggaagcgg gcggttggag tcggcgccca
     4201 gcacgttgcc ttactgccgg atgccgagtc gtggctgatc gcgcggcttg ctgacgccgt
     4261 cgacgagggc gccgcacgcg cgctgacggt tgcggttatc ggcgggcgcg ggggcgcggg
     4321 cgcgagcacc ctcgccgctg ccctcgcggt caccgccatg cgaggcggac gtcgggtcat
     4381 gctcgtcgac gcggatccgt acggcggcgg cgctgatctg ctcttcgggg gtgcgcacgc
     4441 ggcgggcctg cgctggtgcg acatcgctgg tgccagcggc cggatcggcg gcgaggagtt
     4501 gcacgcggcg ttgcctcata tcggctcact cgcggtgttg tcctatgacc gcggcgatcc
     4561 cctcgactta cgagccgacg cgatgtcaag cgtgctcgca gccgccgctc gggtg
//
LOCUS       DATCBJ010000096         3144 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_511639, whole genome shotgun sequence.
ACCESSION   DATCBJ010000096 DATCBJ010000000
VERSION     DATCBJ010000096.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 3144)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3144)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3144
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_511639"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..932
                     /locus_tag="VII50_02000"
     CDS             <1..932
                     /locus_tag="VII50_02000"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEY5049645.1"
                     /translation="DVLGWSMGSMIAQALAMRHPTDVAKLVLCASWPGTGAAVVPAQT
                     AIDALKSGQPSEVRGVLYPADQVFSYDAYVGALSTYPRAPSASASTITQQDGAITGWW
                     AGTDAAGRNPAGISVPTLIADGASDQLDNVSNSNALAKLIPTATVQLYPDAGHAFLFQ
                     EGAPFAFLVESFLSGPPAPAPDATVSSVFQTGETNVAAATRTSQAAVKALSSSPTSAQ
                     LAAIEAAYAGAITVFDDQLLNLAVSGGVDDAVKALVDANEKLVMDALALSVQSNSTAA
                     SWAARATADNEAVQAASAAVRKALGLPAASQSP"
     gene            1173..2234
                     /locus_tag="VII50_02005"
     CDS             1173..2234
                     /locus_tag="VII50_02005"
                     /inference="COORDINATES: protein motif:HMM:NF039482.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HEY5049646.1"
                     /translation="MISRIGLVVHSGKQAAMTAADQVRQWAAARAIPCVDVDVWNASD
                     NGTRRNSTQEAERAGNPDLIVTVGGDGTFLRGARVAAPTGAMVLGVDVGRLGFLTEIE
                     ADDLAQALDALHAGAVQVDARLTLTMRSSRPLEIPQGMQALLRYGRGPALPPPPQRAG
                     DAADVGWGVPLDIMALNDVVFEKLSRDRQVNVAVYVTGRLFALYSADALIVATPTGST
                     AYSFAAGGPIVSPQLDALIFTPVAPHMAFNRSLVLSARDQRVGVRVLEQSGQVAVTVD
                     GQLRGVLDPGDWVSVYAGPERAKLARLTDLDFLGRVRGRFGLADSAAALADGTAPAIY
                     DPGEPLPDDLAHPGPPDIR"
     gene            complement(2268..>3144)
                     /locus_tag="VII50_02010"
     CDS             complement(2268..>3144)
                     /locus_tag="VII50_02010"
                     /inference="COORDINATES: protein motif:HMM:NF022517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2867 domain-containing protein"
                     /protein_id="HEY5049647.1"
                     /translation="AIQPIASTDMTRYLIGAIDVAPGVYEVGGADVTSYREMITAYAA
                     ARGLRRRRIVDVPYLTPRLSSYWLDLVTPVDRRVSHALIESLVTEVVVEDHERTDAAF
                     NIKPMGLVAALSAALDDQALELDHDLLGRDGGLLDGVYTVRVAVPVSGTASARLDADL
                     GRIGGSYSWYGLTFAWKGRALLGRVAGEHWKLGSPTEIVRGAAVDWWQVARREPGILV
                     LRATRWFPGEAWLGYQCTDHELIQVGSLRPKGIPGFVYWKMLQPVHRQVFRALARHRV
                     ARAATHEDQVLRTQG"
BASE COUNT          560 a         1074 c         1014 g          496 t
ORIGIN      
        1 ccgacgtgct cggctggtcg atgggcagca tgatcgcgca ggcattggcc atgcggcacc
       61 cgaccgacgt cgcgaagctc gtactgtgcg cgagctggcc aggaaccggc gccgcggtcg
      121 tacctgcgca aacagcgatc gacgcgctca agagcggtca gcccagcgag gtgcgcggcg
      181 tgctgtatcc cgccgatcaa gtttttagct acgacgcgta cgtaggagcg ctgtcgacct
      241 acccaagagc gccctcagcg tcggcgagca cgattaccca acaggacggc gccatcaccg
      301 gttggtgggc gggcacagac gcggccggtc gcaaccccgc gggtatctcg gtaccaacgc
      361 tgatcgccga cggagcgagc gatcagctgg acaacgtgtc caacagcaac gccctggcaa
      421 agctgatccc cacggccaca gtgcagcttt atcccgatgc aggtcacgcc ttcttgttcc
      481 aagaaggcgc gccgttcgcg tttctcgtcg agtcctttct gagcggcccg ccggcaccgg
      541 cacccgacgc aaccgtgagc agcgtgttcc agaccggcga gaccaacgtg gccgcggcca
      601 cacgaacgtc gcaagccgca gtcaaggcgc tgtccagcag cccgacgtcc gcgcaactcg
      661 cggcgatcga agcggcctat gccggggcga tcaccgtatt cgacgaccag ctgctcaacc
      721 tcgccgtatc tggcggcgtc gacgacgccg tcaaagcact cgtcgacgcc aacgaaaagc
      781 tggtcatgga cgcactcgcg ctgtcggttc agtcgaactc cacagctgcg agctgggcgg
      841 cacgtgccac ggccgacaat gaggcggtac aggcagccag cgccgcagtg cgaaaggccc
      901 tcgggcttcc cgccgcgagc caatcgcctt agctacacgt ggtcaggcgc cgcgctgctc
      961 cctgccatcg ctatctccgt caaccacgcc agggtaaccg cgcccgcaag cggagaccgg
     1021 gccgcaatgc tcgccggggc ggtggcggct atgttggcga ccgtagttgc gtggcgcgcc
     1081 cgagaggcaa tccccgctat ccgtcgggga agcaccgcca ccgagcgcga gcaaccggcc
     1141 ggacggcggc cccggcatct ggaggctgcg cggtgatctc acgtatcggg ctcgttgttc
     1201 acagcggcaa gcaggccgca atgaccgccg cggatcaggt gcgccaatgg gccgctgctc
     1261 gggcaatccc atgcgtcgac gtcgacgtgt ggaatgcctc cgacaacggc acccgacgca
     1321 actcgacgca agaggccgag cgtgccggca accccgatct gatcgtgacc gtcggcggcg
     1381 acggcacgtt cctgcggggc gcacgtgtcg cggcaccgac cggcgccatg gtcctcggcg
     1441 ttgacgtagg ccgacttgga ttcttgaccg agatcgaagc tgacgacctt gcgcaggcgc
     1501 tcgacgcgtt acacgccggc gcggttcaag tcgacgcccg gctcacgctg acgatgcgct
     1561 cgtcacgccc gttggaaatc ccgcagggca tgcaggcgct gctgcgctac ggccgtggtc
     1621 cagccctgcc cccgccgccg cagcgtgccg gggacgcagc cgatgtcggc tggggcgttc
     1681 cgctcgacat catggccttg aacgacgtgg tctttgagaa gctgtcgcga gaccggcaag
     1741 taaacgtggc tgtctacgtc accggccggc tgttcgcctt gtattcggcg gacgcactca
     1801 tcgtcgccac gccgacagga tcgaccgcct acagcttcgc cgcaggcggt cctatcgtgt
     1861 ctcctcagct agacgcgctg atcttcacgc ccgtcgcgcc ccacatggcg ttcaacagaa
     1921 gtctcgtgct ctccgcaaga gaccaacgcg tcggtgtgcg agtgctcgag cagtcaggcc
     1981 aagtcgcagt cacggtggat ggacagctgc gcggcgtcct ggaccccggc gattgggtca
     2041 gcgtgtacgc cggccccgaa cgcgcgaagc tcgccagact caccgacctc gatttcctcg
     2101 gccgagtgcg cggacggttc gggctcgccg actccgcggc cgcactagcc gacggaacag
     2161 cgccggcgat ctacgacccc ggcgagccac tacccgacga cctcgcccat cctgggccac
     2221 cggacattcg gtagtccccg accgcagccc cctgaaaggc gacgttctta tccttgggtg
     2281 cggagcacct gatcctcgtg tgttgctgcg cgtgccaccc tgtgtcgagc aagcgctcgg
     2341 aagacttgac ggtgcacagg ttggagcatt ttccaataga cgaagccagg gattcctttc
     2401 ggacggagcg agccgacttg gatcagctcg tggtccgtgc attgatagcc gagccatgct
     2461 tccccgggaa accagcgagt cgcacgtaat acgaggatgc cgggttcacg tcgagcgacc
     2521 tgccaccagt cgacggctgc gcctcggact atctccgttg gcgagccgag cttccagtgc
     2581 tcccccgcca cacggccgag gagcgcgcgc cccttccatg cgaacgtgag gccataccag
     2641 ctgtagcttc cgccgatccg gccgagatcg gcgtcgagac gcgctgatgc ggtgcctgaa
     2701 acggggacgg cgactcgaac cgtgtagaca ccgtcaagga gaccgccgtc gcgaccaagg
     2761 aggtcgtgat caagctcaag cgcctggtcg tcgagtgcag cggagagcgc ggcgacgagg
     2821 cccatcggct tgatgttgaa ggccgcgtca gttcgctcat gatcttcaac tacgacttcg
     2881 gtcacgagac tctcgatgag cgcgtggctg acgcggcgat ccaccggagt gacgagatct
     2941 agccagtacg acgacaatcg gggtgtcagg tatggcacgt cgacgatccg ccgacgccgc
     3001 agaccgcgcg cggccgcgta agcagtaatc atctcgcgat acgacgtgac gtcggcgccg
     3061 ccgacctcgt acacgccagg cgcgacgtcg atcgctccga tcagatagcg ggtcatgtcg
     3121 gtcgaggcga tcggctggat cgcg
//
LOCUS       DATCBJ010000097         2466 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_505496, whole genome shotgun sequence.
ACCESSION   DATCBJ010000097 DATCBJ010000000
VERSION     DATCBJ010000097.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2466)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2466)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2466
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_505496"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1980
                     /locus_tag="VII50_02015"
     CDS             <1..1980
                     /locus_tag="VII50_02015"
                     /inference="COORDINATES: protein motif:HMM:NF037660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HEY5049648.1"
                     /translation="FAGNDRYIVDYLVDEVLQRQPDHVRTFLLTTSVLERLSGPLCDA
                     VTQRHDSRAMLETLDHANLFVIPLDDIREWYRYHRLFADVLQAHLLETRPDEIAALHV
                     RASAWYQHNGDASEAIRHAMAGHDYDRAADLVELAIPVMRRIRQEATLHGWLKSLPAD
                     VIRARPVLSVGLAGALLAIGEFDGVEARLQDAEGWLSPASGVGSSDQATEMVVANEEE
                     FGRLPAAIALYRAAGALAHGDVPATVAHAQLALDLSPVDDHLPRAAAAGLLGIAYWIT
                     GDSRLEAGHQAWAECVAGLYRAGHVADTFGCSIGMADIRRVQGRLGDAMRTYEQALAH
                     ASAHGGPTLRGTADMYVGISEICSERDDMPAALEHLRHSQELGEHLGLPQNPYRWRVA
                     MARVREAEGDLSGALELLNDAERVYRGDFFPNVRPIPALRARLWIAQGSLDEALGWAH
                     EQGLSADDDLSYMREFEHITLARVLLAQHGKAHDERALHDATGLLQRLLEAAEEGGRT
                     GSVIEILVLQALAQQAVGAVSDALMTLERALTLAQPQGYVRIFVDEGQPMSALLRAAT
                     KEGIARDYVRRLLAAFTKTDTDRPVDQGLIEPLSERELDVLRLLATDLNGPGIARTLV
                     VSLNTVRTHTKNIYTKLDVNDRRAAVRRAGELDLL"
     gene            2066..2338
                     /locus_tag="VII50_02020"
     CDS             2066..2338
                     /locus_tag="VII50_02020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5049649.1"
                     /translation="MSETPSDSRWDDAGQYEVRLKGHLDPRWSAWFDGLILSEDSDGT
                     TVICGAVIDQAALHGLLQKVRDIGLPLVSVTRVDPNHPDLHRFDPR"
BASE COUNT          457 a          787 c          807 g          415 t
ORIGIN      
        1 ttcgcgggca acgaccggta catcgtcgac tacttggtcg atgaggttct gcaacgtcag
       61 ccggatcacg tgcggacctt cctgctcacg acatccgtgc tggaacgact gagtggcccg
      121 ctgtgtgatg cggtgaccca gcggcacgac agcagggcaa tgctggagac gctggaccac
      181 gccaacctgt tcgtgattcc actcgacgac atccgcgagt ggtatcgcta tcaccgcctc
      241 ttcgcggacg tattgcaggc gcacctgctc gagactcgac ccgacgagat tgccgcgttg
      301 cacgtgcgtg cgagtgcgtg gtaccagcac aacggcgacg cgtccgaagc gatccgccac
      361 gcgatggccg gccatgatta cgaccgagcg gctgacttgg ttgagctagc gatcccggtc
      421 atgcggcgga tccgccagga ggccacactc cacggttggc tgaagtccct tcccgccgac
      481 gtgatccggg ccaggccggt gctcagcgtg ggcctggctg gtgcgttgct ggcaatcggg
      541 gagttcgacg gcgttgaagc tcgactgcag gacgccgagg ggtggctgtc gccggcgagc
      601 ggcgtcggtt cgtcggacca ggccacggag atggtcgttg ccaacgagga ggaatttggg
      661 cggctgcctg cggcaatcgc gttgtatcgc gccgctggcg cgctggcgca cggcgatgtg
      721 ccggcaacgg tggcgcatgc ccaactggcg ctcgacctgt ctcctgtgga cgaccacctt
      781 cctcgggcgg ccgcagcggg cctattggga atcgcgtact ggatcacggg ggattctcga
      841 cttgaggcag gccatcaggc gtgggcggaa tgcgttgccg ggctctaccg cgccgggcac
      901 gttgccgaca cgttcggttg ctcgattggg atggccgaca tccggcgtgt gcaaggtcgt
      961 ctcggtgacg cgatgcgtac ctacgagcag gcattggcgc atgcgagcgc gcacggcggc
     1021 ccgacgctgc gtggcaccgc agatatgtac gtcgggatca gcgagatctg cagtgaacgc
     1081 gatgacatgc cggccgctct ggagcatttg cggcacagcc aggagttggg ggagcacctc
     1141 gggttgccgc agaatccgta ccgctggcgg gtcgcgatgg ctcgcgtacg ggaggccgag
     1201 ggtgacctga gcggcgcact cgagctgctc aatgacgccg agcgcgtata tagaggcgac
     1261 ttcttcccca acgttcgacc gatccccgcg ttgcgggcac gattgtggat cgcgcaaggg
     1321 tcattggatg aagcgctcgg atgggcgcat gaacaaggcc tctctgccga cgacgacctc
     1381 agctacatgc gcgagttcga gcacatcacg ctggctcgcg tgctgctggc acagcacggg
     1441 aaagcgcatg acgagcgagc actgcacgat gcaacgggct tgctgcagcg gctgcttgag
     1501 gctgcagaag aaggggggcg gacggggagc gtcatcgaga tcctggtgct gcaggcgctt
     1561 gcgcagcagg ctgtgggtgc agtctcagat gccctcatga cgctcgaacg agcactgaca
     1621 ttggcgcagc cacagggcta cgttcgcatc ttcgtcgacg agggccagcc gatgtcggcc
     1681 ctgctaagag cggcgacgaa agaaggaatc gcccgggact atgtccggcg actgctggcc
     1741 gccttcacca agaccgacac cgaccggccc gtcgatcagg gcttgatcga gccattgagc
     1801 gaacgcgaac tggacgtgct ccggctgctc gccaccgacc tgaacggacc tggaatcgcc
     1861 cggaccctcg tcgtctcgct caacaccgtg cggacgcaca ccaagaacat ctacacgaag
     1921 ctcgacgtga acgaccgccg ggccgccgtc cggcgagcag gggaactcga cctgctgtag
     1981 atcaccacat caatcaccac acatggtgat gccggctcat cacgttcgct cctagctttc
     2041 cctccagaag aactggagga aatcaatgag cgaaacgcca tcggacagca gatgggatga
     2101 cgccggccag tacgaggttc gcctcaaggg ccacctcgat ccgcgctgga gcgcctggtt
     2161 cgacgggctg atcctgtccg aggacagcga cggcaccacc gtcatctgcg gcgcagtcat
     2221 cgaccaggcc gcgctccatg gcctgctcca gaaagtccgc gacatcgggt tgccgctcgt
     2281 ctctgtgact cgcgtcgacc ccaaccaccc cgacctccac cgattcgacc ctcgctagcc
     2341 ctcaccccac aacaggagac cgacatgtcg accaccgttc aaggcacagc agcgaagcgc
     2401 accccgatga caccgttgag gaagaaggcg ctcgtcgccg gcatcttcta cgtggtcacc
     2461 ttcatc
//
LOCUS       DATCBJ010000098         2224 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_246838, whole genome shotgun sequence.
ACCESSION   DATCBJ010000098 DATCBJ010000000
VERSION     DATCBJ010000098.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2224)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2224)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2224
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_246838"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..626)
                     /locus_tag="VII50_02025"
     CDS             complement(<1..626)
                     /locus_tag="VII50_02025"
                     /inference="COORDINATES: protein motif:HMM:NF017051.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HEY5049650.1"
                     /translation="MLVVGLSHRSAPLPLLETASLSVDAAGKLLDDVVASPAVDEAML
                     LSTCNRVELYASVDKFHAGVAALSELLSRHIGVGFDELSPQLYVHYEDRAAQHLFAVT
                     SSLDSMLVGEHQILGQVRAAFRTARDRGDAGSILHDVVERALHAAKRVHAETGIDAVG
                     TTLIEAGLRLAGTHGLQGRRAVVVGAGSMASIAVAALRAAGAGELVVA"
     gene            complement(629..1372)
                     /locus_tag="VII50_02030"
     CDS             complement(629..1372)
                     /locus_tag="VII50_02030"
                     /inference="COORDINATES: protein motif:HMM:NF003992.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0051775 - response to redox state [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HEY5049651.1"
                     /translation="MSASALPTHREPASREHARSATVSSAGTAQARGIPEASIARLPL
                     YLRALQSMADRGVTTVSSQELAVASGVNSAKLRKDLSHLGSYGTRGVGYEVAYLVYQI
                     SRELGLTQDWAVAIVGVGNLGRALANYGGFASRGFRAACLVDADPAQVGQRVGGLVVH
                     HLDDLERLIAEHRIAIGVITTPASAAQRVADRLVAAGIRSVLNFAPTVLTVPPDVELR
                     KVDLSTELQILAFHEQRRVTCAADAAVSL"
     gene            complement(1456..1734)
                     /locus_tag="VII50_02035"
     CDS             complement(1456..1734)
                     /locus_tag="VII50_02035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin family protein"
                     /protein_id="HEY5049652.1"
                     /translation="MSNIDSLSAPPPGPTIRVVLYTRPGCHLCVAARAVVERVTAEFG
                     EPFAERDITLDPQEMGAYADSIPVVLVDGVQVDFWRVSEDRLRAALRR"
     gene            1851..>2224
                     /locus_tag="VII50_02040"
     CDS             1851..>2224
                     /locus_tag="VII50_02040"
                     /inference="COORDINATES: protein motif:HMM:NF012415.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HEY5049653.1"
                     /translation="MTTLRVRRTARWVAALSLTVTALVGSAAFASSAFASPAGDLATA
                     TNTARVSAGLAPLTINAQLNAVAQAWANKLAAAGTLSHNPAVRTQVADWTVLGENVGM
                     AGDVPTVQAAFMHSPEHKANIL"
BASE COUNT          413 a          779 c          688 g          344 t
ORIGIN      
        1 ctggcgacga ccaactcgcc ggcaccggcc gcgcgcaacg cggccacggc gatggatgcc
       61 atcgagccgg ccccgacgac gacggcacga cgtccctgta gcccatgcgt tcctgctaac
      121 cgcaggcccg cctcgatcaa cgtggtgcct actgcgtcga tgccagtctc agcgtggact
      181 cgcttggccg catgcagagc gcgctcgacg acgtcgtgca agatgcttcc ggcgtcaccg
      241 cggtctcgag cagtgcgaaa cgccgccctc acctgaccaa gaatttggtg ttcaccgacc
      301 agcatcgaat cgaggctgga ggtcaccgcg aacaggtgtt gcgctgcacg atcttcgtag
      361 tgcacataca actgcggtga cagttcgtcg aatccgacgc cgatgtggcg cgacaacaac
      421 tcggaaagcg ccgcaacccc ggcatggaat ttgtcgaccg acgcatagag ctcgaccctg
      481 ttgcaggtcg acagcaacat cgcctcatcg acggccggcg aggccacgac atcatcgagc
      541 aacttgcccg ccgcatcgac tgacaacgaa gccgtctcga gtaagggcaa cggtgcactg
      601 cggtgcgaga ggccaacgac gagcaagctc acagcgacac cgccgcgtca gcagcgcacg
      661 tcacgcgacg ctgttcgtgg aacgcgagga tctgcagctc ggtggagagg tcgacctttc
      721 gcagttcgac gtccggcgga acggtcagga ccgtcggcgc gaagttcaac acgctgcgga
      781 tgccagcggc taccaaacga tcggccactc gttgcgccgc gctcgccggc gtggtgatca
      841 ccccgatcgc aatccggtgc tcggcgatca accgttccaa gtcgtcgaga tggtggacga
      901 ccaagccgcc cacgcgctgc ccgacctgcg ccgggtcggc atcgacgaga cacgcggcgc
      961 gaaacccgcg actggcgaag ccgccgtagt tcgccagagc ccgaccgagg ttgcccaccc
     1021 ccacgatcgc gaccgcccag tcctgggtca gcccgagctc gcgagagatc tggtaaacga
     1081 ggtaggccac ctcatagccg acccctcgcg tgccgtacga acccaggtgc gacaggtcct
     1141 tgcgcaactt ggccgagttg acacccgacg ccacggccag ttcctgcgac gaaacagtcg
     1201 tcaccccgcg gtcagccatg gactgcagcg cgcgcaggta taacgggagc cgggcgatcg
     1261 aggcttctgg gatgccgcga gcctgggcgg tgccggcgct acttacggtc gcgctacgtg
     1321 catgctcgcg actggcaggc tcgcgatggg taggtagggc acttgcagac acgctgcttc
     1381 cagacacgcg ggctccgact ttctccgggt cgtgcccagg gtacgtgttt gtgaacacgc
     1441 gcacaaaagc gccgatcacc gccgaagagc cgcccgaagc cgatcttctg agacccgcca
     1501 gaagtcgacc tggacgccgt caacgaggac aaccgggatc gagtcggcgt acgcgcccat
     1561 ctcctgcgga tccaacgtga tgtcccgctc ggcaaagggc tcaccgaact cggccgtgac
     1621 cctctcgacc accgccctcg ccgcgacgca caggtgacag ccgggccggg tgtagagcac
     1681 cacccggatg gtcggcccgg gcggcggcgc gctcaaggaa tcgatattcg acacgccccc
     1741 aaggctaggc cgccccgctc agtaggccga cagggtgatg ttgccgtggc atgtccgatt
     1801 tgtcgcattt gccgttcaag cgttttgcgg gcctcgccga cctatagatc atgaccactc
     1861 tccgtgttcg ccgtacggct cgatgggtcg ctgccttgtc actgacagtt accgcgctcg
     1921 tgggctcagc tgctttcgcg tcttccgcgt tcgcgagccc cgcaggcgac ctagccaccg
     1981 ccacgaacac ggcccgcgtc tccgcgggct tagcgccact gaccatcaac gcccagctca
     2041 acgccgtcgc tcaggcgtgg gcgaacaagc tcgccgccgc cgggacgctt tcccacaacc
     2101 ctgccgttcg gacgcaggtc gccgactgga ctgtccttgg ggagaacgtc ggcatggcgg
     2161 gcgacgtgcc caccgtgcag gccgccttca tgcacagccc ggagcacaag gccaacatcc
     2221 ttga
//
LOCUS       DATCBJ010000099         2148 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_63466, whole genome shotgun sequence.
ACCESSION   DATCBJ010000099 DATCBJ010000000
VERSION     DATCBJ010000099.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2148)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2148)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2148
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_63466"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..933
                     /locus_tag="VII50_02045"
     CDS             <1..933
                     /locus_tag="VII50_02045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HEY5049654.1"
                     /translation="DEIVKIAATRPDIVAITAAMLHPVGLAAFAKAYPNRIVDVGIAE
                     QHAVTSAAGMAMGGLHPVFCVYATFLNRAFDQVLMDVGLHRLPVTFVLDRAGVTGDDG
                     ASHNGMWDLSILSLVPGMRIALPRDGARLRELLAEVVAIDDGPTAIRFPKGGLPHEIE
                     EVDRIGGLDILRRDPDADVLIVAFGPMVELALDVAGRLGDQGMSSTVLDPRWVLPVNP
                     AIAGLAASHSLVVTIEDHGRQGGAGSTVAMALREAGVATPVQVQSIPQRFLAQGKRAE
                     VLHDIGLTAQEIARQVVESVAGIDALDQPQTVTD"
     gene            complement(972..1850)
                     /locus_tag="VII50_02050"
     CDS             complement(972..1850)
                     /locus_tag="VII50_02050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5049655.1"
                     /translation="MSTPVGGPGIESLKDSTALRRCTLAVSVVHEIDLIPTDQGIVLL
                     GVPDIPVSFAQIADAIGNVDPADSVAQDRLHRWLRARRAIADKSLDELAETIRPVALP
                     VGHDLHPGPRWVRQPVLGGSLDLGIGFVGLLADQPELVFVTPPSVLSAAGIDPSPWWR
                     GAMEYLENMGALATTRWRRDPRSPLRPMGDCDVLTLLASRVLRGALCDDAGGMRAIAA
                     PVRTRGWLDLSRIDAAFALTAAAIADDESRGFARPVLLTIDEVSMVADGGRPAEIVLR
                     DPASDETPWLRDVLYH"
     gene            complement(1931..>2148)
                     /locus_tag="VII50_02055"
     CDS             complement(1931..>2148)
                     /locus_tag="VII50_02055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ribonuclease D"
                     /protein_id="HEY5049656.1"
                     /translation="PLADEHGLPAENLLQPDAVRRLAWRPPSDLLAGPVGADLRGFGA
                     REWQIALTAGPLARALLRAATHDVVAN"
BASE COUNT          351 a          687 c          753 g          357 t
ORIGIN      
        1 gacgagatcg tcaagattgc ggcgaccagg cccgacatcg tcgcgatcac cgctgcgatg
       61 ttgcatccgg tcggcctcgc cgcgtttgcg aaggcttacc caaatcgcat cgttgacgtc
      121 ggcatcgcag agcaacacgc agtgacgtcg gcggctggga tggccatggg tggtctgcac
      181 ccggtgttct gcgtttacgc cacgttcttg aaccgcgcat tcgaccaagt cttgatggac
      241 gtcggtttgc atcgcttgcc ggtgacgttc gtgctcgacc gggctggtgt tacaggcgac
      301 gacggggcga gccacaacgg catgtgggat ctgtcgatcc tgtcgttggt ccccggcatg
      361 cgcatcgcgc tgccccgtga tggtgctcgc cttcgcgaac tgctggctga ggttgttgcg
      421 attgatgacg gcccgacggc aatccggttc cccaagggcg ggctgccgca cgagatcgag
      481 gaggtcgacc gcatcggtgg cctcgacatc ttgcgtcgcg accccgacgc cgacgtgctc
      541 atcgtggcgt tcggaccaat ggtcgaactc gccctcgacg tcgcgggccg gctcggcgat
      601 cagggcatgt cctcgactgt gcttgacccg cggtgggtgc tccccgtcaa tccggcgatc
      661 gccggcctcg cggcgtccca ttcattggtt gtgacgatcg aagaccacgg ccgtcaaggt
      721 ggtgcgggtt caacggtggc gatggcgctg cgcgaggccg gcgtcgcaac tccggtacag
      781 gtgcagtcga tcccgcagcg gttccttgct cagggcaagc gtgctgaggt gcttcatgac
      841 atcgggctga cagcgcagga gatcgcccgc caggtcgtcg agtcggttgc cggaatcgac
      901 gctcttgatc aaccgcagac ggtgaccgac tagtagttca ccggggatcg cggtttcgtg
      961 ctccgcaatt tttagtgata gaggacgtcg cgtagccagg gcgtttcgtc actcgccggg
     1021 tcacgcagca cgatctctgc gggccgaccg ccatcagcaa ccatcgacac ctcgtcgatc
     1081 gtcagcagca ccggccgcgc gaagccacgg ctttcgtcgt ccgcgatcgc ggccgcagtc
     1141 agggcaaacg cggcgtcgat ccgggagagg tcgagccagc cgcgggtgcg aaccggcgcg
     1201 gcgatcgcgc gcattccgcc ggcatcgtcg cacaacgcac cgcgcaggac gcgtgatgcg
     1261 agcagggtaa ggacgtcaca gtcgcccatc ggccgcagtg gcgaccgtgg gtcgcggcgc
     1321 cagcgtgtcg tcgccagtgc acccatgttc tcgaggtact ccatcgcgcc ccgccaccat
     1381 gggctcgggt cgatacctgc cgcggaaagg acgctgggcg gcgtgacgaa cacaagctca
     1441 ggctggtctg cgagcaagcc tacgaagccg atgccgaggt cgagggagcc gccaagaacg
     1501 ggctgccgaa cccagcgcgg gcccgggtgt aggtcgtggc cgaccggcaa cgcaaccggc
     1561 cggatcgtct cggcaagttc gtcgagggac ttgtccgcga tcgcgcgccg ggctcgcaac
     1621 cagcggtgca ggcgatcctg agcgaccgaa tcggccgggt cgacattgcc gatggcatcc
     1681 gcgatctgcg caaaactgac gggaatgtcc ggaacgccga gcagaacaat gccctggtcg
     1741 gtcgggatca ggtcgatctc gtggaccacg ctaaccgcga gcgtgcagcg acgaagcgcg
     1801 gtgctgtcct tgaggctctc gatccccggg ccccccacgg gcgttgacat gcacagacgg
     1861 tacctgcgcg tgagcggccg tgggtagcgc gtacgggtga atcgatccgg cgacgcgcgt
     1921 cagcacgtcg tcagttggcg accacgtcgt gagtagcggc ccgaaggagc gcacgggcaa
     1981 gcggccctgc tgtgagcgcg atctgccatt cgcgggcacc gaacccgcga aggtcggccc
     2041 cgaccggtcc ggccagcaga tccgagggtg gtcgccaggc gagccggcgg acggcgtcgg
     2101 gctggagcag gttctccgcg ggaaggccgt gctcgtcggc gagggggg
//
LOCUS       DATCBJ010000100         3224 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_321118, whole genome shotgun sequence.
ACCESSION   DATCBJ010000100 DATCBJ010000000
VERSION     DATCBJ010000100.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 3224)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3224)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3224
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_321118"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1158)
                     /locus_tag="VII50_02060"
     CDS             complement(<1..1158)
                     /locus_tag="VII50_02060"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HEY5049657.1"
                     /translation="MVADAAQVRYDNGEVLDKGLKGGALSLVSSIVIGVASTAPAYSL
                     AATLGFVAILAGTQTVIVVILAFVPIFLISFGYKELNRREPDCGTTFTWGTKAFGPKT
                     GWMGGWGILAADILVMASLSQVAGQYFFLLIGAHGIGGNATSGWVLLVGIIWIVLMTW
                     ICYVGIEISANIQKVLLAIEVVMLAVFSITAIVKTYGNNAPAGSSHISLAWFNPAHMS
                     VSHFVQAILLMLFIYWGWDSAVSVNEETEDKEKTPGRAAVISTILLVAIYAVVAIAVV
                     GFAGVGSTGNGLTNSDNSSDVISILGSAAFGAHGAGLFFSKLLVLMVLTSAAASTQTT
                     ILPTARTSLAMAAYKAIPKAFSKIHPRFLTPSVSTLAMGGVSIVLYVVFNFL"
     gene            1338..2378
                     /gene="speB"
                     /locus_tag="VII50_02065"
     CDS             1338..2378
                     /gene="speB"
                     /locus_tag="VII50_02065"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR01230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008783 - agmatinase activity [Evidence
                     IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HEY5049658.1"
                     /translation="MTRYGAMFGPDVTFLGVDRCDLADPASFAEADVVIVGAPFDGGA
                     SHRPGARFGPQAIRSTDYLPHDGSRPHLALRVDALRDLRVLDAGDVEMPPGEIERALA
                     SLEDAVATVAAAGAIPVVLGGDHSIALPDVTGVARHHGFGRVAVLHFDAHADTDDIQF
                     GSLYGHGQPMRRLIESGAARGDRFLQIGLRGYWPEPETLDWMATQRMRSYEMTEIGVR
                     GLPAVLDEAFGIALDDCDAVFLSVDIDVCDPGHAPGTGTPEPGGLTARELLDSVRRIC
                     RELPIVGVDVVEVSPPFDHAEITAMLANRVVLEALSGLAARRRDSATAVTFDPAMPLL
                     SGRHDGTGRTTL"
     gene            2375..>3224
                     /locus_tag="VII50_02070"
     CDS             2375..>3224
                     /locus_tag="VII50_02070"
                     /inference="COORDINATES: protein motif:HMM:NF015400.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase NADP-binding
                     domain-containing protein"
                     /protein_id="HEY5049659.1"
                     /translation="MKILLVGAGGVGGAITGIAARRLFFDAMVVADYDEARAQRAAER
                     TGDPRFSAIRIDASDESAVASALREHECDVLLNATDPRFVMPLFRAALSAGAHYLDMA
                     MSLSRPHAEQPYALTGVKLGDEQFELSGKWDDAGRLALVGIGVEPGLSDIFARYAADH
                     LFSHIDEVGIRDGANLTVEGYDFAPTFSIWTTIEECLNPPVVWERNRGWYTTEPFSEP
                     EVFDFPEGIGPVECVNVEHEEVLLVPRWVEAERVTFKYGLGDEFIGVLKTLHKLGLDR
                     VDKIKVA"
BASE COUNT          566 a         1014 c         1062 g          582 t
ORIGIN      
        1 aaggaagttg aacacgacgt acagcacaat cgatacgccg cccattgcca gcgttgagac
       61 acttggtgtc aggaagcgcg ggtgaatctt tgaaaaggct ttcgggatcg ccttgtatgc
      121 agccatcgcg agcgacgtgc gggcggtcgg caagatcgtc gtctgcgtcg atgcagccgc
      181 cgaggtgagc accatcaaaa cgagcagctt ggagaagaac aggcccgcac cgtgcgcacc
      241 aaacgcggcg ctgccgagga tgctgatgac gtcgctggag ttgtcgctgt tcgtgagccc
      301 gttacccgtg gaacccaccc cggcgaaccc gacaacggcg atcgccacga ccgcatagat
      361 cgccaccagc aggatcgtcg agatgactgc ggcgcgaccc ggcgtcttct ccttgtcctc
      421 cgtctcttcg ttcaccgaca ccgcactgtc ccagccccag tagatgaaca gcatcagcag
      481 aatggcttgg acaaagtggc tcacgctcat gtgggccggg ttgaaccacg ccaggctgat
      541 gtgactggag ccagcagggg cgttattgcc gtaggtcttc acgatggcgg tgatggagaa
      601 tacggcgagc atgacgacct cgatcgccag cagcaccttc tggatgttgg ccgagatctc
      661 gatgccgacg tagcagatcc aggtcatcaa gacgatccag atgatgccga ccagcaagac
      721 ccaaccgctc gtcgcgttgc caccaatgcc gtgggcgcca atgagcagaa agaagtattg
      781 gcccgcaacc tgagagaggc tggccatgac caggatgtcc gcggcgagga tgccccagcc
      841 acccatccag ccggtcttcg gcccgaacgc cttggtaccc caggtgaacg tcgtgccgca
      901 gtctggttcg cgccggttca actccttgta cccgaacgag atcaggaaga tcgggacgaa
      961 ggccagaatg acgacgatga ccgtctgggt gccggccagg atcgcgacaa agcccagcgt
     1021 cgccgcgagg ctgtaggccg gcgctgtcga tgcaacgccg atgacgatgc tcgagacgag
     1081 gctcagtgca ccgcccttga gccctttgtc gagcacttct ccgttgtcgt agcggacctg
     1141 cgcagcgtcg gcgaccatga gcacctcggg gagcgatgtg ggcggattga tggcaccagc
     1201 ggttttgaac cctccggtta tcgcactgat ttcatcggat gacaagggat tccgtggtct
     1261 cgttctgata gcgcgaccgt ttcggtggta gcgtcccgcc atgcgagcgt tgcctgtcgg
     1321 tcggctgccg gtgtgaagtg acccggtatg gggcgatgtt cggcccagat gtcacatttc
     1381 taggcgtcga tcgttgcgac ctggccgacc cggcatcgtt cgcggaggcg gacgtcgtca
     1441 tcgtcggagc gccgttcgac ggtggcgcct cacaccgccc cggcgccagg ttcggtccgc
     1501 aggcgatccg ctcgaccgac tacctgccgc acgacggatc ccggccgcat ctcgccttgc
     1561 gcgtcgatgc gctgcgcgac ctgcgggtcc tcgacgctgg cgatgtcgag atgccgcccg
     1621 gcgagatcga gcgcgcgctg gcaagccttg aggatgccgt ggccacggtc gcggcagcag
     1681 gagcgattcc ggttgtgctt gggggagatc actcgatcgc actccccgat gtcacgggcg
     1741 ttgcccgcca ccacggattc ggtcgcgtcg ccgtgttgca cttcgatgcg cacgcggaca
     1801 ccgacgatat ccagttcggc tcgctctacg gtcatggcca gccgatgcga aggctcatcg
     1861 agtctggagc tgcgcgaggc gaccggtttc tccagatcgg tctgaggggc tactggcccg
     1921 agccggaaac gctcgactgg atggcgacgc agcgcatgcg gtcgtacgag atgaccgaga
     1981 tcggcgtgcg cgggctcccg gcggtgctcg acgaggcgtt cggcatcgcg ctcgacgact
     2041 gcgacgccgt gttcctatct gtcgacatcg acgtctgcga cccaggtcat gcccccggta
     2101 cgggcacgcc agaacccggt ggcttgacgg cgcgggagct gcttgattcc gtccgccgaa
     2161 tctgtcgtga gctgcctatc gtgggcgtcg atgttgtcga ggtgtcaccg ccgttcgacc
     2221 acgcggaaat aaccgcgatg ctcgcgaatc gggtggtact cgaggcgctg tcgggcttgg
     2281 cggctcggcg tcgcgactca gcaaccgcag tgacgttcga cccggcgatg ccattgctct
     2341 ccggccggca cgacggtacc ggaaggacaa cgttatgaag atcctcctcg taggtgccgg
     2401 cggtgtaggc ggagccatca ccggaatcgc cgcacgccgg ctgttcttcg acgccatggt
     2461 ggtggccgac tatgacgagg cccgggcgca gcgggcggcg gagcgcaccg gcgacccgcg
     2521 attctctgcg atccgcatcg acgcctcgga cgagtcggct gtcgcgtcgg cgctgcgtga
     2581 acacgagtgt gacgtgctgc tcaatgcgac agatccgcgt ttcgtgatgc cgttgttccg
     2641 ggccgccttg tcggccggcg cgcactacct cgacatggcg atgtcgctgt cgcggccgca
     2701 cgccgagcag ccctacgcgc tgaccggggt aaagctcgga gacgagcagt tcgagctgtc
     2761 cgggaaatgg gacgacgcgg ggcggctcgc tctcgtcggc atcggtgtcg aacccggctt
     2821 atccgatatc ttcgcccgct acgccgccga ccatctgttc tcacacatcg acgaggtcgg
     2881 catccgcgac ggcgcgaacc tgaccgtcga gggctacgat ttcgcgccga ccttctcgat
     2941 ctggaccacg atcgaggagt gcctgaaccc gccggtcgtg tgggagcgga accgcggttg
     3001 gtacacgacc gagccattca gtgagcccga ggtgttcgac ttccccgagg ggatcgggcc
     3061 cgtcgaatgc gtgaacgtcg agcacgagga ggtgctcctt gtaccgcgtt gggtcgaggc
     3121 ggagcgggtc accttcaaat atggcctagg cgacgagttc atcggcgtcc ttaagaccct
     3181 tcacaagctc ggtctcgacc gcgtcgacaa gatcaaggtc gctg
//
LOCUS       DATCBJ010000101         4195 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_99178, whole genome shotgun sequence.
ACCESSION   DATCBJ010000101 DATCBJ010000000
VERSION     DATCBJ010000101.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 4195)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4195)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4195
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_99178"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(25..1467)
                     /gene="sucB"
                     /locus_tag="VII50_02075"
     CDS             complement(25..1467)
                     /gene="sucB"
                     /locus_tag="VII50_02075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase, E2 component,
                     dihydrolipoamide succinyltransferase"
                     /protein_id="HEY5049660.1"
                     /translation="MSVSVTMPRLGESVTEGTVTRWLKKEGDQVAVDEPLLEVSTDKV
                     DTEIPSPVAGILSKITVAEDETVEVGSELAVIDDGSAGAAPAAAPALADADAAPAAVA
                     DPPAAEAAPAVEAAPAPDAEEPAPQPEASDQSALVEPPADAPVPAPPAAAEPATEAGT
                     DAGGDGGDPSYVTPLVRKLATEHDVDLSTLTGTGVGGRIRKQDVLAAAEAARAAKAAA
                     AAPRAPAATTAPAGEPSPAAAALRGRTEKMSRLRGVLASRLVESLQTAAQLTTVVEVD
                     VTRVSRLRDGAKVDFEAREGVKLSFLPFFALATVEALKQHPIVNASIDTAAGTITYHD
                     AEHLGIAVDTPRGLLVPVVRDAGDLNLGGLARKIADVANRTRNNQVNPDELSGGTFTV
                     TNTGSRGALFDTPILLPPQVGILGTGAVVKRPVVVDDGDLGETIAIRSMVYLALTYDH
                     RLVDGADAARFLGTIKARLEEGRFEADLGL"
     gene            complement(1504..2886)
                     /gene="lpdA"
                     /locus_tag="VII50_02080"
     CDS             complement(1504..2886)
                     /gene="lpdA"
                     /locus_tag="VII50_02080"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009074768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HEY5049661.1"
                     /translation="MADENGTFDLVILGGGSGGYAAAFRASELGLSVALIEKDKVGGT
                     CLHRGCIPTKALLHAAEIADGARDGASVGVKSTFEGIDVPAVHAYKDGVVNRLYKGLQ
                     GLVKANKITYIEGEGRLVSPTAVEVGGQRYEGRHILLATGSAPRSLPGLDIDGDQIIT
                     SEHALRLDRVPASVVVLGGGVIGVEFASVWKSFGSEVTIVEALPHLLPLEEESSSKLL
                     ERAFRRRGINFELGVRFEGVKTTDAGVVVTLEGGKTIEAEYLLVAVGRGPVSAGLGYE
                     ENGVATERGFVLVDEYCRTNVPTISAVGDLIPTLQLAHVGFAEGILVAERLGGLSPVP
                     IDYDGVPRITYSEPEVASVGLTEAKAKEKYGDNLKSFTYDLAGNGRSQILKTSGAVKV
                     IAVADGPVVGIHIVGARVSELIAEAQLIYNWEALPSEVAQLIHPHPTQTEAIGEALMA
                     LAGKPLHVHS"
     gene            complement(2993..>4195)
                     /locus_tag="VII50_02085"
     CDS             complement(2993..>4195)
                     /locus_tag="VII50_02085"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF002073.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HEY5049662.1"
                     /translation="EVAISLGTAADGTISDDAVKAVAEGALLGAYSYRRYRRATLHGY
                     LPPVRSVVVLTTDPVPRSTKQAVARAKIVCDAVCAARDLVNTPPSDLHPREFADIATE
                     EANRAGLAIEVLDEKALKKGGYGGIIGVGQGSVNPPRLVRLAYRHPKATRTLALVGKG
                     ITFDSGGLSLKPAAPMEWMKSDMGGAAAVLMAMVAIARLKLPVNVTGWAPLAENMPSG
                     SAQRPSDVITIYGGKTVEVLNTDAEGRLILADALARASEELPDLIVDAATLTGAQLVA
                     LGTRMSGVMANDDDFRTAVVDAATRAGEHMWPMPLPPELRKSLDSSIADIANMGEPFG
                     GMLVAGSFLSEFVGTRDGEPITWAHLDIHGPAFNQGDAYGYTPKGGTGAAVRTFVQIA
                     DDVAAGRL"
BASE COUNT          638 a         1397 c         1448 g          712 t
ORIGIN      
        1 ctcttcatgg ccggcgcacc ggatttagag gccgaggtcg gcttcgaagc gcccttcctc
       61 gagtcgcgcc ttgatcgtgc ccaggaaccg ggccgcgtcg gcgccgtcga cgagccggtg
      121 gtcataggtc agagcgaggt agaccatcga gcggatcgcg atcgtctcgc cgaggtcgcc
      181 atcgtcgacg accaccggac gtttgacgac agctccggtg ccgagaatgc cgacttgcgg
      241 aggcagcagg atcggagtgt cgaacaacgc gcccctgctg ccggtattcg tcacagtgaa
      301 ggtgccgccc gacaactcgt cgggattgac ctggttgttt cgggtgcggt tcgcaacatc
      361 ggcgatcttg cgcgcgagac cgccgaggtt gaggtcgccg gcgtcacgca cgaccggcac
      421 cagcaggccg cgcggggtgt cgacggcgat cccgaggtgc tcggcgtcgt ggtaggtgat
      481 ggtgccagcg gcggtgtcga tgctggcgtt gacgatcgga tgctgcttca acgcttcgac
      541 ggttgcgagc gcgaaaaatg ggaggaacga cagcttcacg ccttcgcgcg cctcgaagtc
      601 gaccttcgcg ccgtcgcgca gccgcgaaac gcgcgtgacg tcgacctcga cgaccgttgt
      661 cagttgcgcg gcggtctgca gggattcgac aagacgagac gcgaggacgc cgcgaagccg
      721 ggacatcttc tcggtgcgac cgcgcaacgc ggcggccgca gggctgggct ccccggccgg
      781 cgccgtcgtt gcagctgggg ccctcggcgc ggcggcggca gctttggctg ctcgcgccgc
      841 ttcggccgcc gcgagcacgt cctgcttgcg gatccggccg ccgacgccgg taccggtcag
      901 ggtgctgagg tcgacgtcat gctcggtggc cagcttgcgc accagcggcg tcacgtagga
      961 cgggtcaccg ccatcgccac ctgcgtcggt gccagcttcg gtcgcgggtt ccgcggccgc
     1021 cggcggtgcg ggtacaggcg cgtcggccgg tggttctact agggcggatt ggtcgctggc
     1081 ttcgggttgc ggcgctggct cctcggcgtc cggcgcgggt gctgcctcga ctgctggtgc
     1141 tgcctcggcc gcaggcgggt ccgccactgc tgccggcgcg gcgtccgcgt cggccagtgc
     1201 cggcgccgcc gcgggcgcag ccccggcaga gccgtcgtcg atgacggcga gctctgagcc
     1261 gacctcgacg gtctcgtcct cggcgacggt gatcttggac aggatcccgg ccactggcga
     1321 agggatttcg gtgtcgacct tgtcggtcga gacctcgagc agcggttcgt cgacggcgac
     1381 ctggtcgccc tctttcttca accagcgggt gacggtgccc tcggtgacgc tctccccgag
     1441 gcggggcatc gtgacggaga ccgacatgag ggctatgact cccgagtgga cgtagtgatg
     1501 gggctagctg tgcacgtgga gcggcttgcc ggccagggcc atgagggcct cgccgatggc
     1561 ttcggtctgc gtcgggtgcg ggtggatcag ctgagcaacc tcactcggca gcgcctccca
     1621 gttgtagatg agctgggctt cggcgatcag ttcgctcacg cgagcaccga cgatgtgaat
     1681 acccacgaca gggccgtccg caaccgcgat tactttgacg gcgcccgacg tcttcaggat
     1741 ctgactacgg ccattccccg cgaggtcgta ggtgaagctc ttcaggttgt cgccgtactt
     1801 ctccttggcc ttggcctcgg tgagtccgac cgaagcgacc tcaggttcgg agtaggtgat
     1861 gcgcggaacg ccgtcgtagt cgatcggcac gggggacagg ccacccaggc gctcggcgac
     1921 caggatgcct tcggcgaagc cgacatgggc aagttgcagt gtggggatca ggtcgccgac
     1981 ggcggagatg gtcggaacat tggtccggca gtactcgtcg accagcacga agccacgttc
     2041 ggtcgcgacg ccgttctctt cgtagccgag tccggcagac accggtccac gaccgaccgc
     2101 aaccaagagg tattcagcct cgatcgtctt gccgccctca agggtcacca ccacgcccgc
     2161 gtcggtggtc ttgactcctt cgaagcgcac gccgagctcg aagttgatgc cgcggcggcg
     2221 gaaagcgcgc tcgagcagct tcgaacttga ctcctcttcc aagggcagca ggtgggggag
     2281 cgcctcgacg atcgtgacct cggagccaaa ggacttccag acactcgcga actcgacgcc
     2341 gatgacgccg ccaccaagga cgaccacaga ggccggcacc cggtcgagcc gcagtgcgtg
     2401 ctcactggtg atgatctggt cgccgtcgat gtcgagacca ggcaacgacc tcggcgcgga
     2461 cccggttgcg agcaggatgt gccggccttc ataccgctgg ccgccgacct cgaccgccgt
     2521 cggggagaca agccgaccct cgccctcgat gtaggtgatc ttgttcgcct tcacgagacc
     2581 ctgcagaccc ttgtagaggc ggttcacgac gccgtccttg taggcgtgca cggcaggaac
     2641 gtcgatgccc tcgaaggtcg acttgacgcc gaccgatgca ccgtcgcgcg cgccgtcggc
     2701 gatctcggcc gcgtgcagca gcgccttggt cgggatgcag ccgcggtgca ggcaggtgcc
     2761 gccgaccttg tccttttcga tcagcgcgac cgagagcccg agctcgctcg cgcgaaaggc
     2821 agccgcgtag ccgccgctcc cgccgccgag gatgaccagg tcaaacgtgc cgttctcgtc
     2881 tgccacgagc actccttacg cgagtcgttg cgcaccgacg gtgcagcgcc tgcgggatcg
     2941 ccgtggcgtc aacatcattc tcccacgccg gtctagccgc cgggatcgcg cgtcagagcc
     3001 gaccggccgc tacgtcgtcc gcgatttgca cgaaggtccg gacggcggca ccggtgccgc
     3061 ccttgggggt gtacccgtag gcgtcgccct gattgaacgc cgggccgtgg atgtcgaggt
     3121 gcgcccaggt gatcggctcg ccgtcgcgcg tcccgacgaa ctcagacagg aagctccccg
     3181 cgaccagcat gccgccaaac ggctcgccca tgttcgcgat gtcggcgata gaggagtcga
     3241 gtgactttcg aagctcgggt ggcaagggca tcggccacat gtgctcgccg gctcgggtcg
     3301 cggcgtcgac caccgcggtc cggaaatcgt cgtcattggc catgacgccc gacatacggg
     3361 tgccgagcgc gacgagttgc gcgcccgtca atgtcgccgc gtcgacgatg aggtccggta
     3421 gctcttcact cgcgcgggcg agggcgtcgg ccagaatcaa cctgccctcg gcgtcggtgt
     3481 tcagtacctc gacggttttc ccgccgtaga tggtgatcac gtcggacggc cgctgtgccg
     3541 aacccgacgg catgttttca gcgagtggcg cccagcccgt gacgttgacc ggcagcttca
     3601 gtcgggcgat cgcgaccatc gccatgagca ccgccgcggc gccgcccatg tcggacttca
     3661 tccactccat cggcgccgcc ggcttgagcg acaagccgcc ggagtcgaac gtgatgccct
     3721 tgccgacaag cgcaagcgtt ctcgtcgcct tcggatgccg gtaggccagc cgcaccaggc
     3781 gcgggggatt taccgagccc tgcccgacgc cgatgatgcc gccgtagccg cccttcttca
     3841 gggctttctc gtcgagaacc tcgatcgcca ggccggcccg attcgcttcc tccgtcgcga
     3901 tgtcggcgaa ctcgcgtggg tgcagatcgg acggcggtgt gttgacgaga tcgcgggccg
     3961 cgcacaccgc atcgcagacg atcttggccc gcgcgactgc ttgcttcgtc gatcgcggta
     4021 ccggatcagt agtgagaacc acgacggatc tgaccggagg caggtatccg tgcagcgtgg
     4081 cgcggcggta tcgccggtag gagtacgcgc cgagcaaggc gccctcggca acggctttca
     4141 ccgcgtcgtc gctgatcgtg ccatcggcgg cggtgcccag cgagatcgcg acctc
//
LOCUS       DATCBJ010000102         8631 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_320827, whole genome shotgun sequence.
ACCESSION   DATCBJ010000102 DATCBJ010000000
VERSION     DATCBJ010000102.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 8631)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8631)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8631
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_320827"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1559)
                     /locus_tag="VII50_02090"
     CDS             complement(<1..1559)
                     /locus_tag="VII50_02090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HEY5049663.1"
                     /translation="MPIPAQTAGLLNRSDEPQTNGRRRLGELLVSHGLLTDEQVRHAL
                     SEQERMEGGRRRRLGRVVVDEGFLTEGQLAYALADLLHLELVDLGELTLDLSVARTLP
                     RRVAERHCMLVIGRDDHAIRVATADPTNVVALDDVRLYTGATTVTVVVSTESQIQTEL
                     RRVWAILDQDAELSNIADEPEAVDEHDLENAADQGPTVRLATSIVTEAVRSRASDIHV
                     EPQVDGLRIRYRIDGLLRDIMKVPRNSSAALVSRLKIVSGLDIAERRLPQDGRTRLTV
                     DGNGVDARVSTLPSVHGEKVVIRLLASGDHVPPVTQLGLDEQQLEALLAGTASPQGLV
                     LITGPTGSGKTHTLYSVLSHMATPDKNVVTLEDPVEIQLPGITQVQTNERAGLSFSKG
                     LRSILRQDPDVVLVGEVRDSETAELALQASMTGHLVLTTLHTNDAVAALTRLVDMGIQ
                     PFLIASSLSLVVAQRLVRRPCADCAAPYMPSARVLTLLGLSEADLVDATPMRGRGCGE
                     CGGTGYRGRTG"
     gene            1726..2982
                     /locus_tag="VII50_02095"
     CDS             1726..2982
                     /locus_tag="VII50_02095"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY5049664.1"
                     /translation="MRAPALLRTNRDFRLLWFGESVSELGTTVTYVALPFVALIVLHA
                     TTFEVASLAAVETIFWPFIALPIGVWVDRVSRRRVLLAADAGRALALVSIPIAAALGD
                     LTLVQLYVVGVVAGVLTIFFSVAYPSYLQSVVTKDELVEGNSLLTGAEQVAHISGPAL
                     GGVLVQAMGAAYALIADVASFVVSVAAVFAIRAEEPPPVTERRAMRNEVGEGVRFLLD
                     HKVLRAFIVAAATSNLFAGGAQAITVLFLVRVVHVRPGVVGLLLSVGALGGLLGAVLA
                     RRLIRLFGDGRTMVLAAVVEALGAYLVPATAQGWRLALFCVGQAIASIAIVAFNVVGG
                     SYRQEIVPARLMGRVMAANRMVTWGALPIGAFGAGALAANLGVRPALWILAAGLSLAP
                     LSLLVAGFATIRTLPRESPLVVETLL"
     gene            complement(2979..3422)
                     /locus_tag="VII50_02100"
     CDS             complement(2979..3422)
                     /locus_tag="VII50_02100"
                     /inference="COORDINATES: protein motif:HMM:NF039693.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6221 family protein"
                     /protein_id="HEY5049665.1"
                     /translation="MIDNLTEWLLLQIAEDEMKARSAGSEPWRWRVVDAPTQRDALVR
                     PNGTVCADSWGADRQETGFRGAEAKMFIERFNPERVLADCDSKRRMLARLQNLAKAWW
                     TTTRSADEARGVIREMAMSYSNRPGYRDEWRPDLGMRPLQAVSSN"
     gene            complement(3620..4810)
                     /gene="glgA"
                     /locus_tag="VII50_02105"
     CDS             complement(3620..4810)
                     /gene="glgA"
                     /locus_tag="VII50_02105"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003796502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - glycogen (starch) synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase"
                     /protein_id="HEY5049666.1"
                     /translation="MRVALLTREYPPDVYGGAGVHVEYLARELRRFIDVEVHCFGKPR
                     SEPGVFAHLADPTLLELGANPALLTMSTDLSIAGATGDADILHSHTWYANFAGHVAKL
                     LQDAPHVVTTHSLEPLRPWKAEQLGGGYALSSFCERTAIEAADAVVAVSAGMADDVLR
                     CYPLVDPGRLHVIHNGIDTHEYAPTTDTSVLVDHGIDLDRPIVIFVGRITRQKGLPYL
                     LKAAHSFDQDAQLVVLASSADTPEIADEVEDGIVALQETRDGVFWIRRMLPKLELIQV
                     FTAATVFVCPSIYEPMGIVNLEAMACETAVVATRTGGIPEVVADGETGVLVPIDPDPA
                     DPYGAPRDPDAFAGAFAEAVNALVRDSARARAMGVAGRSRAVEHFSWTAIAERTRDLY
                     ATLL"
     gene            5011..6249
                     /gene="glgC"
                     /locus_tag="VII50_02110"
     CDS             5011..6249
                     /gene="glgC"
                     /locus_tag="VII50_02110"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HEY5049667.1"
                     /translation="MANKVLGIVLAGGQGTRLLPLTADRAKPAVPFGGIYRLIDFVLS
                     NLVNGGFLKIVVLTQYKNHSLDRHITTTWRMSTLLGNYVTPVPAQQRVGPRWFSGSAD
                     AIYQSLNLVYDERPDYILVFGADHIYRMDPRQMLDQHIASGAGVSVAGIRAPRKEASA
                     FGVIQTVQQGPDAGRRIEAFLEKPQDIEGIGLPDSPDEVFASMGNYCFTTAALLDAVN
                     RDAADERSAHDMGGNIIPMFVERGDAQVYDFNDNVVPGATDRDRAYWRDVGSLDAYYD
                     AHMDLISVHPIFNLYNENWPIYTQHRPLPPAKIVHEDTRVVDSMLSNGVIVADSTVAG
                     SVLSPNVHIESGADIDGSVLLENVRVGRGAVIHNAIIDKNVEVPSGAKIGVDLVRDRQ
                     RFTVSDHGIIVIGKNAVVDV"
     gene            complement(6266..6802)
                     /locus_tag="VII50_02115"
     CDS             complement(6266..6802)
                     /locus_tag="VII50_02115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1003 domain-containing protein"
                     /protein_id="HEY5049668.1"
                     /translation="MARRETHRAGLDQPKSDRRVTLRPSYDPETFGRLSERIARFFGT
                     ARYLAMQTLVVIAWISYNGVQTFLFYHDHGSKPFDIYPFILLNLVFSTQAAYAAPLIL
                     LAQTRQAERDRIQSEDDRSRNERNIADTEYLAREVASLRIALGDVATRDFIRGELQRM
                     LEDLDERRVAGDEQRDAL"
     gene            complement(6789..8120)
                     /locus_tag="VII50_02120"
     CDS             complement(6789..8120)
                     /locus_tag="VII50_02120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019510465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEY5049669.1"
                     /translation="MSGPMSRVFVARLAGVVVFDPNGDQVGRVRDVIVLLRPGTQQPR
                     VLGLVVEVPPRRAIFLPITRVTGIEAGAVMTTGTVSLRRFAKRDSETLVLGDLLDRKV
                     TLTESGEVVTVTDVAMERSRARDWLLTKVAVRSDVRRVRRRGAIRVVDWDAVTGLSLH
                     EHAQGAANLLAAFDQLRPADLAGVMHDLSEKRRAEVAAALDDERLADVLEELSEEEQV
                     EILATLEGERAADVLEAMGPDDAADLLAELPEAEKERLLGLMLPEEANPIRRLLDYSW
                     NTAGGLMTTEPVILDPTATVAEALAHVRNPDLSPATASQVYVVRPPTDTPTGRYLGTA
                     HLQRLLREPPATLVSSIIDNDIAPLRPDVSLPAITSHLATYNLVAAPVVDDEDRLLGA
                     VTVDDVLDHLLPDGWRDTDPSEAVNGGNGAPHEDGAPHEDGSSEGATNGTP"
     gene            8368..>8631
                     /locus_tag="VII50_02125"
     CDS             8368..>8631
                     /locus_tag="VII50_02125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5049670.1"
                     /translation="MRGWPGRHRADAALRSESKRVHDAHAGDYEGAMAVVRRALAQLD
                     VARAHRDELVVPAVLRATHERIDDALARAAAAATAVYIALFEAA"
BASE COUNT         1351 a         2785 c         2954 g         1541 t
ORIGIN      
        1 attccggttc tgccgcgata cccggttccg ccgcactcgc cgcaaccgcg gccgcgcatc
       61 ggtgttgcgt cgaccaggtc ggcttcgctg aggccgagca acgtgagtac gcgggcgctc
      121 ggcatgtagg gcgcggcgca gtcggcacac ggacgccgga ccagccgctg tgcgacgacg
      181 aggctcaatg acgacgcgat cagaaagggt tggatgccca tgtcgaccag tcgggtcagc
      241 gcggccaccg cgtcgttcgt gtgcagggtg gtcagcacga ggtggcccgt catcgacgcc
      301 tggagggcca gttctgccgt ctcggagtca cgtacttcac caacgagcac gacatccggg
      361 tcctgccgaa ggatcgaccg caggcccttg ctgaacgaca ggccggcgcg ctcgttcgtc
      421 tgcacctggg tgataccggg aagctgaatc tctaccgggt cttcgagggt gacgacgttc
      481 ttgtccgggg tggccatgtg agacagcacc gagtacagcg tgtgcgtctt gcccgagccg
      541 gtcgggcccg tgatgagcac gagtccttgc gggctcgcgg tgccagcgag cagggcttcc
      601 agttgctgtt cgtcaaggcc gagttgggtc accggcggta cgtggtcgcc gctggccagc
      661 agccggatga cgaccttctc gccatgcact gaaggcagcg tgctgacgcg ggcgtcgacg
      721 ccattgccat ccacggtaag tcgcgtgcgg ccgtcctgcg ggaggcgtcg ctcggcaatg
      781 tcgagcccgg agacgatctt gaggcggctg accagcgcgg ccgaggagtt gcgcggcact
      841 ttcatgatgt cgcgaaggag gccgtcgatg cggtacctga tccgcagccc gtcgacttgc
      901 ggctcgacgt ggatgtcgct ggcccggctg cgcacggctt cggtgacgat gctcgtcgcg
      961 agccggacgg ttggtccctg gtcggccgcg ttctcgagat cgtgctcatc gacggcttcg
     1021 ggttcgtccg cgatattcga cagctctgca tcctggtcga ggatcgccca gacgcggcga
     1081 agctcggtct gaatctgcga ctcggtgctc accaccaccg tgaccgttgt cgctccggtg
     1141 tagagccgga cgtcgtcgag ggcgaccacg ttggtcggat ctgcggttgc caccctgatc
     1201 gcgtggtcgt cgcgtccgat caccaacatg cagtggcgct cggcgacgcg gcgcggcagt
     1261 gtgcgcgcga cgctcaggtc cagcgtgagt tcaccgaggt cgaccagttc gaggtgcagc
     1321 agatcggcga gcgcgtacgc gagctgaccc tccgtgagga agccctcgtc gacgaccacc
     1381 cggccgagtc tgcgtcgccg tccgccctcc atgcgctctt gttctgacag cgcgtggcgc
     1441 acctgctcgt cggtcagcag accgtgcgaa accagcagct caccgagacg tcggcgcccg
     1501 ttagtttgcg gctcgtcgct gcggttgagc aaaccagcgg tttgggcagg tatcggcacg
     1561 tccgaactcc tcacgtttcc tacattgtcg ggcgcaggga acggggctac accggctaag
     1621 tgcctcctag agatcctccg aacggtcggg tggcgctggt ctgcgcgcta cgccgatcgc
     1681 ataacgcgtt gacccgaacg gcggggtcga cacagtgtgg agcccatgcg tgcgcccgct
     1741 ctgctccgga cgaaccgcga cttccggttg ctgtggttcg gggagtccgt cagcgaactc
     1801 ggcaccaccg tgacctacgt agcgctgccg ttcgtcgcgt tgatcgtgtt gcatgcgacc
     1861 acgttcgagg tcgcgtccct ggcggccgtg gagaccatct tctggccgtt catcgcactg
     1921 ccgatcggag tatgggtcga tcgcgtgtcg cgacgtcggg tgctactcgc ggccgatgcg
     1981 gggcgcgcgc ttgcgctggt atccattccg atcgcggccg cgctcggcga cctgaccctg
     2041 gtccagctct atgtcgtcgg cgtggtggcg ggcgtgctca cgatcttctt ttccgttgcc
     2101 tatccgtcgt acctgcaaag cgtggtcacc aaagacgagc ttgtcgaagg caactcactg
     2161 ctgaccggcg ccgagcaggt cgcgcatatc tcgggtcccg cgctcggcgg tgtgctcgtc
     2221 caggcaatgg gcgccgccta cgccctgatt gccgacgtgg ccagcttcgt cgtttcagtg
     2281 gcggcggtgt ttgcgatccg cgccgaggag ccgccaccgg taacggagcg gcgggccatg
     2341 cggaacgagg tcggcgaggg agttcgcttt ctcctggatc acaaggtgct gcgggcattc
     2401 atcgtcgccg ctgccacgtc gaatctcttc gccggcggcg cgcaggccat cacggtgctc
     2461 tttttggtgc gggtcgttca tgtgcggccc ggtgtcgtgg gcctgctgct atctgtcggc
     2521 gcgctcggtg gactgctcgg cgcagtactg gcgcgcaggt tgatccggct gttcggcgac
     2581 ggtcgaacga tggtgctggc cgccgtcgtc gaagcgttgg gtgcctacct ggtgccggcg
     2641 accgcgcagg ggtggcggct cgcactcttc tgcgtaggtc aggcgatcgc cagcattgcc
     2701 atcgtggcgt tcaacgtcgt gggcggcagc tatcggcagg aaatcgtgcc ggcgaggttg
     2761 atgggccggg tcatggccgc gaaccggatg gtgacctggg gagccttgcc gatcggcgca
     2821 ttcggcgctg gcgcactggc cgcgaacctt ggcgtgcggc cggcgttgtg gatcctcgcg
     2881 gccgggttgt cgctcgcccc actgtcgctg ctcgtcgctg ggttcgcgac cattcgcacg
     2941 ttgccgcgtg aatcgccgtt ggtggtcgag acgctcctct agttcgagga gacggcctgc
     3001 agcgggcgca tgccgaggtc cgggcgccac tcatcacggt agccgggacg gttggagtag
     3061 ctcatcgcca tctcacgaat gacgccgcgc gcttcgtcgg cggagcgggt cgtggtccac
     3121 catgcttttg cgaggttttg caggcgggcc agcatccggc gcttcgaatc gcagtcggcg
     3181 agtacccgct cgggattgaa tcgctcgatg aacatcttgg cctcggcacc gcgaaaaccg
     3241 gtttcctgcc ggtcggcgcc ccatgagtcg gcgcacaccg tgccgttcgg ccggaccagg
     3301 gcgtcgcgtt gggtcggggc gtcgacgacg cgccaccgcc acggctcgct gccggcactg
     3361 cgcgccttca tctcgtcctc ggcgatctgg agcagcagcc attccgtcag gttgtcgatc
     3421 acgctgcgtg acggtagcga catgggactc gcggtccttc cggtggagca ggctcgccgc
     3481 ggccgttctc aagagggccg attgcctaca aacacatcat cgaaacggct gacccgactc
     3541 ttgaccggca aacgtgcgat agctcccgaa caacccgatc ggcgtagcgc cgatggcgaa
     3601 ctcagcgctc ccgcgaggtc tacagcaagg tggcgtatag gtcgcgcgtg cgctccgcaa
     3661 tcgccgtcca gctgaaatgc tcgaccgctc ggctgcgacc ggcgacgccc atggccctgg
     3721 cgcgagcgga atcgcggacg agcgcgttca cggcctcggc gaacgccccg gcgaatgcgt
     3781 ctgggtcgcg cggcgcgccg taagggtcgg ccgggtcggg gtcgatgggc accagcacgc
     3841 cagtttcgcc gtccgcgacc acctcgggga taccgccggt ccgggtcgcg acgactgctg
     3901 tttcgcatgc catggcctcg aggttgacga tccccatcgg ctcgtagatc gacgggcaga
     3961 cgaaaaccgt ggccgccgtg aacacctgga tgagctcgag cttcggcagc atccggcgga
     4021 tccagaagac accgtctcgg gtctcctgca gcgccacgat gccgtcctcg acctcgtcgg
     4081 cgatctccgg ggtatcggct gagctggcca gcacgacgag ctgagcgtcc tggtcaaacg
     4141 agtgggcggc cttcaacagg tagggcaggc ccttctgcct ggtgatgcgg ccaacgaaga
     4201 tgacgatcgg ccgatcgaga tcgatgccgt ggtcgaccag caccgaagtg tccgtggtcg
     4261 gcgcgtactc gtgcgtatcg atgccgttat ggatgacatg gagtcggccg gggtcgacga
     4321 gcggatagca acgcaggacg tcgtctgcca tgcccgcaga tacggcgacg acggcgtctg
     4381 ctgcttcgat cgccgtccgc tcgcaaaacg aggaaagggc gtagccgccg ccgagttgct
     4441 ccgctttcca cggccgcagc ggttccagtg agtgcgtggt cacgacgtgc ggggcgtcct
     4501 ggagcagttt cgcgacatgg ccggcgaagt tcgcgtacca cgtgtgcgag tgcaagatgt
     4561 cggcgtcgcc ggtcgcgccg gcgatcgaca ggtcggtcga catcgtgagc agcgccgggt
     4621 ttgctccgag ctcgagcagc gtcgggtcgg cgaggtgcgc aaagacaccc ggctccgacc
     4681 gcggcttgcc gaagcagtgc acctcaacgt cgatgaaccg tcgcagctcg cgggcgaggt
     4741 attcgacgtg cacgcctgcg ccgccataga catcaggggg gtattcgcgg gtgagcagcg
     4801 cgactcgcat gaccggcaac gatagccaag ggggatgtcc cccacgttgc attgggtcaa
     4861 ctgcgaccgt gcgatgggtc aactgcgacc ctgcgatggg tcaactgtga ccgtgggatg
     4921 ggtcaactac aaccgtgcga cggggtcaac cgcgccagaa tctccacccg cggaggcgcg
     4981 aatcgaggcg ccaggaggta ggttcccgac atggcgaaca aggttctcgg catagtcctg
     5041 gcgggtgggc agggcactcg actgttgccg cttactgccg accgcgcgaa gccggcggtg
     5101 ccctttgggg gcatctaccg gctcatcgac ttcgtgctct cgaacctcgt caacggtggg
     5161 ttcctgaaaa tcgtcgttct gacccagtac aagaaccaca gtctcgaccg gcacatcacc
     5221 acgacctggc gaatgtcgac gctcctgggc aactacgtga cgccggtgcc ggcccaacaa
     5281 cgggtcggcc cgcgctggtt ctcgggctca gccgacgcga tctaccagag cctgaacctg
     5341 gtctacgacg agcgacccga ctacatcctg gtattcggcg ctgatcacat ctaccgcatg
     5401 gacccgcgcc agatgctcga ccagcacatc gcctcgggtg caggcgtgtc ggtcgccggg
     5461 attcgggccc cccgcaagga agcatcagcg ttcggcgtga tccagacggt ccagcaaggt
     5521 cctgatgccg gacgccgcat cgaggcgttc ttggagaagc cgcaggacat cgaaggcatc
     5581 ggactgccgg actcacccga cgaagtgttc gcctcgatgg gtaactactg cttcacgact
     5641 gccgcactcc tcgacgccgt gaaccgcgat gcagccgacg aacgatccgc tcacgacatg
     5701 ggcggcaaca tcatcccgat gttcgtcgag cgtggcgacg cgcaggtata tgacttcaac
     5761 gacaacgtcg tgcccggcgc gacggatcgc gaccgggcgt actggcgcga cgtcgggagt
     5821 ctcgacgcgt actacgacgc gcatatggac ctgatctcgg tgcacccgat tttcaatctc
     5881 tacaacgaga attggcccat ctacacccag catcgcccgc tgccaccggc gaagatcgtg
     5941 catgaagaca cccgagtggt cgactcgatg ttgtcgaacg gggtgatcgt cgccgattcg
     6001 acggtggccg gctcggtgct ctcgcccaac gtgcacatcg agagcggtgc tgacatcgac
     6061 ggcagtgtgc tactcgaaaa cgtacgagtt ggccggggtg ctgtgatcca caacgccatc
     6121 atcgacaaga acgtcgaggt cccctccggc gcgaaaatcg gcgtcgacct tgtacgcgac
     6181 cggcaacgat tcaccgtgtc ggatcacggc atcatcgtga ttggcaagaa cgcagtagtc
     6241 gacgtctgac ccggtcgctg gtcggctaga gggcgtcgcg ctgttcgtcg cctgccactc
     6301 gtcgctcgtc gaggtcctcg agcatgcgtt gcagttcacc gcggatgaag tcacgggtgg
     6361 ccacgtcgcc gagcgcgatt cgcaaggacg cgacctcgcg ggcgagatat tcggtgtcgg
     6421 cgatgttgcg ctcgttgcgc gatcggtcgt cctcggactg gatccggtcg cgctcagcct
     6481 ggcgagtctg cgccagcagg atcagcgggg ctgcgtacgc ggcctgtgtc gagaagacca
     6541 ggttcagcag gatgaacgga tagatgtcga acggcttcga gccgtgatcg tggtagaaga
     6601 gaaacgtctg cacgccgttg tagctgatcc acgcgatcac cacgagcgtc tgcatcgcga
     6661 ggtaacgagc ggtcccgaag aaccgcgcga tcctttcgga tagccgcccg aacgtttcag
     6721 ggtcgtaact cgggcgcagc gttacgcggc ggtcggactt cggctggtcg agcccagccc
     6781 ggtgggtttc acggcgtgcc attggtcgca ccttcgcttg agccgtcctc gtggggggcg
     6841 ccgtcctcgt ggggggcgcc gttcccgccg tttactgcct cgcttgggtc cgtgtcgcgc
     6901 cagccatcgg gcagcaggtg gtcgagaacg tcatcgactg tgaccgcgcc aaggagccga
     6961 tcctcgtcgt cgacgaccgg tgcggccacc aggttgtacg tcgccaagtg actggtgatt
     7021 gccggcaacg agacgtcggg acgcagcggt gcgatgtcgt tgtcgatgat ggaactcacc
     7081 agcgtcgcgg gtggttcacg tagcagcctt tgtaggtgcg cggtgccgag gtagcgaccg
     7141 gtcggtgtgt ccgtcggcgg gcgcacgacg tagacctgac tcgcggtggc tggggacagg
     7201 tcggggttgc gcacgtgcgc gagggcctcg gccacggtcg ccgttgggtc gaggatcacc
     7261 ggttcggtcg tcatcaggcc gccggcggtg ttccaggagt agtcgagcag gcggcggatc
     7321 gggtttgctt cctcgggcag catgaggccg agcagtcgct ccttctcggc ctccggcaac
     7381 tcggccagca ggtctgccgc gtcgtccggg cccatcgcct cgagaacgtc agccgcccgc
     7441 tcgccctcga gggtcgccag gatctcgacc tgctcctctt cggacagttc ttccaggacg
     7501 tcggccagcc gctcgtcgtc cagcgccgcg gctacctcag ctctgcgctt ctccgatagg
     7561 tcgtgcatca ccccggccag gtcggcgggg cgcaactggt cgaacgcggc cagcagattc
     7621 gctgctcctt gggcgtgctc gtgcagcgac agtccggtga cggcgtccca gtcgacgacc
     7681 cggatcgcgc cacgccggcg gacccttctg acgtcgctgc gaaccgcgac cttggtcagc
     7741 agccagtcac gtgcgcggct gcgttccatc gcgacgtcgg tgaccgtgac gacctcacca
     7801 gattcggtga gcgtgacctt gcggtcgagg aggtcaccga gcaccagcgt ctcggagtcg
     7861 cgcttggcga agcggcgaag gctgacggtt ccggtcgtca tcaccgcgcc agcctcgatg
     7921 cccgtaaccc gcgtgatcgg gaggaagatc gcccggcgcg gcggcacctc gacgacgagc
     7981 ccaaggacgc gaggctgctg ggtgccgggc cgcagcagca cgatgacgtc gcgcacccgg
     8041 ccgacctggt cgccgttcgg atcgaagacg acaacgccgg cgaggcgcgc aacgaagacc
     8101 ctcgacatcg gcccgctcat gtgcggaggc taccgccgtg tggcccgcgc gccccgcgcc
     8161 tttggcgctg acgcgctgag ccccttctgg cgttgacgcg ctgagcgctt tttggcgttg
     8221 acgcgctgag ccccttctgg cgctgacgcg ctgagccctt ttggcgctga cggccggtcc
     8281 gattgaaaac cggcagtctg gggcacaacc ccaaaaggtc ccacatagcg cacgtagagc
     8341 acgacatgac agcggggagg cgaagcgatg cgaggctggc ccggacgcca tcgggcggac
     8401 gcggcactcc gcagcgaaag caagcgggtg cacgacgcgc acgccggcga ctacgagggg
     8461 gcgatggccg tggtccggcg ggcgctggct cagcttgacg tggcccgcgc acatcgcgac
     8521 gaacttgtcg tgcccgccgt cctccgcgct actcacgagc gcattgacga cgccttggct
     8581 cgggccgcgg cggccgcgac ggctgtctac attgccctct ttgaggcggc c
//
LOCUS       DATCBJ010000103         2001 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_504650, whole genome shotgun sequence.
ACCESSION   DATCBJ010000103 DATCBJ010000000
VERSION     DATCBJ010000103.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2001)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2001
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_504650"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..346
                     /locus_tag="VII50_02130"
     CDS             <1..346
                     /locus_tag="VII50_02130"
                     /inference="COORDINATES: protein motif:HMM:TIGR01727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015440 - ABC-type peptide transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="oligopeptide/dipeptide ABC transporter
                     ATP-binding protein"
                     /protein_id="HEY5049671.1"
                     /translation="ADRIMIMYAGHVVESGPAEAVLADPKHPYTQLLLSAVPDPRAPL
                     AVGAETDKGEPPKVIDPAPGCRFRARCPFVIDECHHVDPQLRLLAPRHFAACHVAKPD
                     ADFLAPAAVTPF"
     gene            complement(347..970)
                     /locus_tag="VII50_02135"
     CDS             complement(347..970)
                     /locus_tag="VII50_02135"
                     /inference="COORDINATES: protein motif:HMM:TIGR03085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03085 family metal-binding protein"
                     /protein_id="HEY5049672.1"
                     /translation="MTNLAASERDSLSDLLNRVGPEAPTLCEGWTARDLTAHLVLRES
                     RPDAVLGVVIRPLSGWTARVQKKLARKDFTELVNRLRGGPPIWSPFRPHAVGELLSNV
                     ELFVHHEDVRRAAAQWEPRVLDSTTSALMWRRSSQMAKHVLRSTPGGIELVRSDTGQR
                     RTVRPGQPVLTLAGAPQELLLYMFGRRDHALVERAGDAEGFAVLEKS"
     gene            complement(967..1665)
                     /locus_tag="VII50_02140"
     CDS             complement(967..1665)
                     /locus_tag="VII50_02140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5049673.1"
                     /translation="MARRALLARRGLAGVIVVAAVAATAALGVRYARTRTPGRLDRYL
                     DDAIQRRVAGHSHLLVKLIDVADPGSVIVICALLSALFLLMNRRRLAILAVLGPGLAG
                     GLVELVLKPLFDRHLGASLSFPSGHTAATASLALVVIIAMIGPSRLPWPFVFRWLIAA
                     AATASVPVIAIALVGGGYHYATDTIGGLLVAIACVLSVALLIDAVAGHPGSHRAVPNH
                     ATQQDGAVLPRVRA"
     gene            1787..>2001
                     /locus_tag="VII50_02145"
     CDS             1787..>2001
                     /locus_tag="VII50_02145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding protein"
                     /protein_id="HEY5049674.1"
                     /translation="MPGSPQERSLGSAQEYDVDLLIVGAGPAGLYAAYYAGFRALSVV
                     IVDSLTEPGGQLAALYPEKMIYDVGGIP"
BASE COUNT          343 a          690 c          667 g          301 t
ORIGIN      
        1 cgccgaccgg atcatgatca tgtacgccgg ccacgtcgtc gaatcgggac ccgcggaggc
       61 ggtgttggcc gaccccaagc atccctacac ccaattgctg ctctcagcag tgcccgatcc
      121 gcgggcgccg ctggcggtgg gcgccgagac cgacaagggc gagccaccga aggtcatcga
      181 cccggccccc ggttgtcgat tccgggcgcg ctgcccgttc gtcatcgacg agtgtcacca
      241 cgtcgatccg cagttgcggc tgctggcacc gcggcacttc gcggcctgcc atgtcgcaaa
      301 accggacgcc gacttcctcg cgcccgcagc agtgacgccc ttctgatcac gacttctcga
      361 gtaccgcgaa gccttctgcg tcacccgcac gctcaaccag cgcatggtcg cgtcgcccga
      421 acatgtacag gagcagttcc tgtggcgcac cagcgagtgt cagcacgggc tgacccggcc
      481 gcacagtgcg gcgttgcccg gtgtcggagc gcaccagttc aataccgccg ggggtggatc
      541 gcaacacgtg cttggccatc tggctcgacc gtcgccacat gagtgcgctc gtcgtgctgt
      601 cgagcacgcg gggctcccac tgcgccgcag cgcgccgcac atcctcgtgg tgcacgaaca
      661 actcgacgtt gctcaggagc tcgccgaccg cgtgcgggcg aaacggcgac cagatcgggg
      721 gcccgcctcg caaacggttc accagttcgg tgaagtcctt tcgcgcgagc ttcttctgca
      781 ccctcgccgt ccagcccgat agcggccgga tcacgacgcc cagaacggcg tcgggcctgc
      841 tttcacgcag cacgaggtgc gcggtcaggt cgcgggcggt ccagccctcg cacaaggttg
      901 gcgcctcggg gccgacgcga ttgagcaggt cactgaggct gtcgcgctca ctggcggcga
      961 gattggtcac gctcttactc taggcagcac tgcgccgtcc tgctgtgtcg cgtgattggg
     1021 cacggcacgg tggctgcccg gatgtccagc caccgcgtcg atcaacaacg cgaccgatag
     1081 cacgcaggca atcgcgacga gcaggccgcc gatcgtgtcg gtggcgtagt ggtagccgcc
     1141 tccgaccagg gcgatcgcga tgaccgggac cgatgcggtc gccgcggcag cgataagcca
     1201 ccggaagacg aacggccacg gcagccgact tggtccgatc atcgcgatga tgacaacgag
     1261 ggcgagcgag gcggtggcgg cggtgtgccc gctcgggaac gacagcgagg cgcccaaatg
     1321 ccggtcgaac agcggcttca acacgagctc gacaagaccg ccagccaagc ccggtccgag
     1381 gaccgcgagg atcgcgagcc ggcgccgatt catgagcagg aacaacgcgg acagcagcgc
     1441 acagatcacg atcacgctgc ctggatctgc gacgtcgatc aatttcacca gcagatgcga
     1501 gtggcccgcc acgcggcgct ggatcgcgtc gtcgaggtac cggtcgagac gacccggtgt
     1561 gcgagtacgc gcatatcgga cgccgagggc agccgtcgcc gcgacggcgg cgacgacaat
     1621 gacacctgcc agaccgcggc gcgcgagtaa cgctcggcgg gccatccctg aagtctacga
     1681 gccgaaacgt cgcgcggccg gacgttcgcg acatgcaccg ggacgtgccg cgaccgcgcg
     1741 cggctggcgt actaccgtgc aggtcacaac ctcttggagg aattcggtgc caggttctcc
     1801 gcaggagcgc agtctggggt ccgcgcagga gtacgacgtc gacctgctca tcgtcggtgc
     1861 aggtccggcc ggcctctacg ccgcctacta cgccggtttc cgcgccctgt cggtggtcat
     1921 cgtcgattcg ctgaccgagc ccggtgggca gctcgcggcg ctgtacccgg agaagatgat
     1981 ctacgacgtc ggcggtatcc c
//
LOCUS       DATCBJ010000104         3300 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_286719, whole genome shotgun sequence.
ACCESSION   DATCBJ010000104 DATCBJ010000000
VERSION     DATCBJ010000104.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 3300)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3300)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3300
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_286719"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(142..456)
                     /locus_tag="VII50_02150"
     CDS             complement(142..456)
                     /locus_tag="VII50_02150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5049675.1"
                     /translation="MSRIDSLRTFIVGHPADPFPRYALALEYKNGDRLTEARDEFAAL
                     MRDHADYVAAYLHAGNTHLALGDRAAARETFERGIEASIRRGDGHARGELESALALVA
                     GP"
     gene            complement(466..864)
                     /gene="panD"
                     /locus_tag="VII50_02155"
     CDS             complement(466..864)
                     /gene="panD"
                     /locus_tag="VII50_02155"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004847071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004068 - aspartate 1-decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HEY5049676.1"
                     /translation="MRRTLFKSKIHRATVTQADLDYEGSVTIDADLMRAADMLPYEKV
                     HIWNRTNGARLETYALEGPAGSGVICVNGAAAHLARPGDMVIIATFAEANDEAEAHAW
                     VPKVVHVDAQNHIAPVEGEIPGPQRRLQRV"
     gene            1023..1886
                     /locus_tag="VII50_02160"
     CDS             1023..1886
                     /locus_tag="VII50_02160"
                     /inference="COORDINATES: protein motif:HMM:NF016163.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF429 domain-containing protein"
                     /protein_id="HEY5049677.1"
                     /translation="MTTRSFVHVLGIDLGGGKGKTTALATLRVDGAAATVVDIAPRNG
                     ALPFYDPALLETIRAYGDETLLCVDAPLTLPPCLRCLVPVCPGQSDCVDPAVLEMRAL
                     ASETDGARGRDARRGKPPITPYTQRTTEVYLSHRTGIVSREGLGQGTGPLAARAAHLL
                     RALADRFRLNENLIEAYPKATLAALGLGTPFKKHLRERETRARILEALADELRFGPGV
                     WRELCIQSDHLFEAVICAFTGFLWARDGWSLPQGAGQREDDARLRDGWIWTPPARSDD
                     VAAPKKSVAEW"
     gene            complement(1968..3179)
                     /locus_tag="VII50_02165"
     CDS             complement(1968..3179)
                     /locus_tag="VII50_02165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011237251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISAzo13 family transposase"
                     /protein_id="HEY5049678.1"
                     /translation="MASETEIRRKWDALSPILDERERRLWAGAEAEHSEDGIAAVERA
                     TGLSRTTIRAGRDELRGGLDPDGVVNVRKAGAGRPSIEEKNPDLVPTLESLVDPVTRG
                     DPESPLRWTSKSTRKLASELAARGMKVSPQKVGELLYAAGYSLQGANKTIEGAQHPDR
                     NAQFEYINGRVDDFHARGQPVISVDTKKKELVGEFKNVGAEWQPSGSPVPVRVHDFID
                     DGGKVIPYGVYDLGRNQAWVNVGVDHDTPAFAVRSIADWWRHMGKKAYPDATELLITA
                     DAGGSNGYRSRLWKTELQSFADRTGLNIGVSHFPPGTSKWNKIEHRLFCHITENWRGR
                     PLVDHETVVQLIGSVRTKTGLVVKAKLDTRLYPIGVEVADEEMDGLKITRDSFHGEWN
                     YAFAPSIRRAA"
BASE COUNT          516 a         1126 c         1072 g          586 t
ORIGIN      
        1 cggcagatgg tcaacagcgg gcgcttacgt ctctccgaag gaccgagcgt ccgcgacgac
       61 acatcacatc tgacggccga acgtggcggt ctcgacgaag cgccgacccg gcacgccggc
      121 agtcgggtac ggccgcgcag gctacggccc ggcgaccagc gccagtgccg actcgagctc
      181 gccgcgggcg tggccgtcgc cgcggcgaat ggaggcttcg atcccgcgct cgaaggtctc
      241 gcgggcagcg gcccgatcgc ccagcgccag gtgggtgttg cccgcgtgca ggtaagccgc
      301 cacgtagtcg gcgtggtcgc gcatgagcgc ggcgaactcg tcgcgcgcct cggtcagccg
      361 atcgccgttc ttgtactcca acgccagcgc gtagcgcggg aacggatcgg ccggatgtcc
      421 gacgatgaag gttcgcaggg agtcgatgcg cgacatgggg cgacgtcaga cgcgctgcag
      481 gcgccgctgc gggccgggaa tttccccttc gaccggcgca atgtggttct gggcgtcgac
      541 gtggacgacc ttgggcaccc aggcgtgtgc ctccgcttcg tcgttggcct ccgcgaaggt
      601 cgcgatgatg accatgtcac ccggacgggc caggtgggcc gccgcgccgt tgacgcagat
      661 gacacccgat ccggcgggtc cttccagcgc ataggtctcg aggcgggcgc cgttggtgcg
      721 gttccagatg tgcacctttt cgtagggcag catgtccgcg gcgcgcatga ggtcggcgtc
      781 gatcgtcacc gagccttcgt agtcgaggtc ggcttgggtg acggtcgcgc ggtgaatctt
      841 cgacttgaag agggtccggc gcatttcgaa gcctccgtcc aggccggcaa attgccggtc
      901 ccggcgtggt tgccgttagg atacaaagtg aagccccgca agtcaaaagc caactcgacc
      961 gtcgggcgac cgacaagtcc gcggttgacg gtggtgaccg aatcccgcac gctcactgag
     1021 gcgtgacaac gcgcagtttc gtccacgttc tcgggatcga cctcggcggg ggcaaaggca
     1081 aaaccaccgc gctcgccacg ctgcgcgtcg atggcgccgc cgccaccgtg gtggacatcg
     1141 ccccccgcaa cggcgccctg cccttttacg acccggccct cctcgagaca atccgcgcgt
     1201 acggcgacga gacgctcctc tgcgtcgacg cgccgctgac gctgccgccc tgcctgcgct
     1261 gtctggtccc cgtctgccct ggccagagcg actgcgtgga tccggcggtg ctcgaaatgc
     1321 gcgcgctggc ctcggagacc gacggggcgc gcggacgcga cgcccggcgg ggcaagccac
     1381 ccatcacgcc ttatacccag cgcacgaccg aggtctatct gtcgcaccga acggggatcg
     1441 tctcccgcga agggctcgga caggggaccg gaccgctggc ggcgcgcgcg gcgcatctgc
     1501 tgcgcgcgct ggccgaccgg ttccgactca acgagaacct gatcgaggca tacccgaagg
     1561 cgacgctggc cgcgcttgga ctcggcacgc cgttcaagaa gcacctgcgc gagcgcgaga
     1621 cccgggcgcg catcttggag gcactggccg acgagctgcg cttcggcccg ggtgtctggc
     1681 gcgagctctg tatccagagc gaccatctgt tcgaggcggt catctgcgcc ttcaccggat
     1741 tcctgtgggc gcgcgacggc tggtcgctcc cgcagggtgc cggacaacgt gaagacgacg
     1801 cgcgcctgcg cgacggctgg atctggaccc cgcccgcccg cagcgacgac gtcgccgccc
     1861 cgaagaagtc ggtcgccgaa tggtagccgc tcccgttcgg tcgggcgtcg cgaccgacgc
     1921 cgtggcgcgc tcagcgtgcg cttaggggcc gatcagaaat tacttgatca agcggcacga
     1981 cggattgagg gagcgaatgc gtagttccat tcgccatgaa agctatcgcg cgtaatcttc
     2041 aggccatcca tctcctcgtc ggcgacctcg acgccgatcg gataaagccg ggtatcaagc
     2101 tttgctttga cgacaagtcc agtcttcgtt cggacgctgc cgatgagttg cactacggtc
     2161 tcgtgatcga cgagcggccg gccacgccag ttctcggtga tgtgacagaa gagccggtgc
     2221 tcgatcttgt tccacttgct ggtgcctggc ggaaaatggg aaacgccgat gttcaggcct
     2281 gttcggtccg cgaaggattg aagctcggtc ttccagagac gcgatcggta gccgttgctg
     2341 cctcccgcgt cagcggtgat cagcagctcc gtggcatcgg ggtaggcctt cttccccatg
     2401 tggcgccacc agtcggcgat cgaccgcaca gcaaacgctg gggtgtcgtg gtcgaccccg
     2461 acgttcaccc aggcttggtt ccgtcccaga tcgtacacgc cgtacgggat caccttgccg
     2521 ccatcatcga tgaagtcgtg cacgcgtaca ggcacgggac tgccgctcgg ctgccactcg
     2581 gcaccgacat tcttgaattc tccgacgagc tccttcttct tcgtgtcgac ggagatgact
     2641 ggctggccgc gggcgtgaaa gtcatccacc cggccgttga tgtactcgaa ctgcgcgttt
     2701 cggtcgggat gctgcgcgcc ctcgatggtc ttgttcgccc cctgcaggct gtagccagct
     2761 gcgtagagca gctcaccaac tttctgcggg ctgaccttca tgccacgcgc agccagctcg
     2821 ctcgccagct tccgcgtgct cttggatgtc catcgcaacg gcgactccgg atcgccccgt
     2881 gtgaccggat cgaccagcga ttccaacgtc ggcacgagat cggggttctt ttcctcgatc
     2941 gatggccggc cggctcccgc tttgcgcacg ttcacgacgc cgtcggggtc gagccctccg
     3001 cgcagctcgt ctcgcccagc acgaatcgtc gtgcgcgaga gcccagtcgc tcgttcgacc
     3061 gccgcaatcc catcttcgct gtgctctgcc tccgctcccg cccaaagccg ccgctcacgt
     3121 tcgtcgagga tcggagacag cgcgtcccat ttccttcgaa tctcggtctc gcttgccatc
     3181 cccaccatag atcatgcccc gagtcgtttg tcgatccccc gctgcgcagt aatcaggtaa
     3241 tttctgagcg gggcctaact cgtcgcgctc tcgcgctggc agcacgaggc tcggatcctc
//
LOCUS       DATCBJ010000105         2702 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_357006, whole genome shotgun sequence.
ACCESSION   DATCBJ010000105 DATCBJ010000000
VERSION     DATCBJ010000105.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2702)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2702)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2702
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_357006"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..831)
                     /locus_tag="VII50_02170"
     CDS             complement(<1..831)
                     /locus_tag="VII50_02170"
                     /inference="COORDINATES: protein motif:HMM:NF024758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HEY5049679.1"
                     /translation="MQAYPPPQSLPEQPPTSSPRSVPSARWLLALTCVLILAVVGAAI
                     FGWSQAHRLASTRGQLATLRASAASEQAQVSALASAVSAQQQSVASLGTQLGVTQTQL
                     ATTQSKLGTTQSQLQTAQTRLGTDEQQLSSTTKQLSVTTKQLPPDLTALATQVSPSVV
                     LITCTTGAGGDTGTGFALALPAASGYATTVITAEHVIDACTDPSTGAFISLARGGTTL
                     PVHLQAFDSGEDVAILDTTAKLPTLKPAAAPVVGEFLMAVGNPLGVNDNVTSGNVSQV
                     Y"
     gene            complement(940..1497)
                     /locus_tag="VII50_02175"
     CDS             complement(940..1497)
                     /locus_tag="VII50_02175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009082406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HEY5049680.1"
                     /translation="MRTLIVTEFISLDGVVEAPGGEATHPHAGWTMAYGVPDSYAFKL
                     KETMDAESMLLGRTTYEGFAAAWPKMSDVFADKMNTMPKHVVTSTLTELEWNATPMAA
                     DVAAVTALKQGGGGPILVHGSATLVRWLLAQHLVDELHLMVFPVMVGGGLRIFPDQRE
                     KLEFELIDLTRYDSGVLLQIYRPVP"
     gene            1528..1896
                     /locus_tag="VII50_02180"
     CDS             1528..1896
                     /locus_tag="VII50_02180"
                     /inference="COORDINATES: protein motif:HMM:NF015925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HEY5049681.1"
                     /translation="MSRRGYGRYDNPELIGCASVPSVFTLVVRFDLPDAAGARAFDEL
                     TAAAVPIIRAHEPGTLVYETYAVEGAPLARVFYEVYRDRDAHAEHERQPRTAAFLTAV
                     RTLVAQTRVEILSRLESGGL"
     gene            complement(1893..>2702)
                     /locus_tag="VII50_02185"
     CDS             complement(1893..>2702)
                     /locus_tag="VII50_02185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5049682.1"
                     /translation="NSVTPDTDRAVVVLRRHRLTGVVALAVTLGGLGLGGCSAINKVK
                     SVAHAVENKKKTMDAFSTKIQSGEAKPFEATYVTTGSTPTTVVYAVQPPKGLSFTNTP
                     TSTSSAGSTGDTNAPSLDIIVNSTGEYSCTPPDTSGSAGKGTWACQMLSPVAADEQNA
                     IFDFYTPAHWINFLKGFSLAAGFAGDKVTASTMTVNAFAMSCVDFATPGEAGESTICT
                     TAQGILGYVKVAGDSTSFEIKSYTSSPSASLFELPPGATVTQVTLPSSPAA"
BASE COUNT          434 a          872 c          907 g          489 t
ORIGIN      
        1 gtacacctgc gacacgttgc ctgacgtcac gttgtcgttg acaccgagcg ggttcccgac
       61 cgccatcagg aattcgccga cgaccggggc cgccgcaggc ttcagcgtcg gcagcttggc
      121 ggtcgtgtcg aggatcgcga cgtcctcgcc cgagtcgaag gcttgaaggt gcaccggcaa
      181 tgtcgtgccc ccgcgcgcca gagaaatgaa cgcgcctgtc gacgggtcgg tgcaggcgtc
      241 gatcacgtgc tcagctgtga tgacggtggt ggcatagccg gacgccgccg gcagcgccag
      301 agcgaatcca gtgccggtgt cgccgccggc ccccgtcgtg caggtgatca ggaccaccga
      361 cggcgagacc tgcgtggcaa gcgccgtgag atccggcggc aactgcttgg tcgtgacgct
      421 cagctgcttg gtcgtcgagc tgagctgctg ctcgtcggtc ccaaggcgag tttgcgccgt
      481 ctgcaactgc gattgcgtcg tacccaactt gctttgggtc gtcgccagct gagtctgcgt
      541 aacgccgagc tgggtgccga ggctggcaac actttgctgt tgtgcactta cggcgctcgc
      601 gagtgctgat acctgcgcct gttccgacgc cgccgaggcg cgcagcgtcg cgagctgacc
      661 gcgcgtgctc gcgaggcggt gcgcttggct ccaaccgaag attgcggcgc cgacgaccgc
      721 cagtatcaag acgcaggtaa gggcgagcag ccagcgcgcg gacggcactg accgcggcga
      781 cgaggtcggc ggctgttcgg gaagcgactg gggcggcggg taggcctgca cgacgtccgt
      841 tctacctcac acagcccggt cgcgcagcgg gcgcgcggcg acattcgcgc tggctccgcc
      901 ggctcgtccc tcgccagccc caccagcgac tagttacttt cagggcacgg gtcggtagat
      961 ctgcagcaag acgccgctgt cgtagcgcgt caggtcgatg agctcgaact cgagcttctc
     1021 ccgttgatcg gggaagatgc ggaggccacc gccgaccatc accgggaaaa ccatcaggtg
     1081 aagttcgtcg acgaggtgct gtgcgagcag ccaccgaacg agggtcgcgc tgccgtgaac
     1141 caagattgga ccgccgccgc cctgcttcag cgcggtcacg gcggcgacgt cggccgccat
     1201 tggagtcgcg ttccactcaa gctcggtgag cgtgctcgtg acgacatgct tgggcattgt
     1261 gttcatcttg tccgcgaaca cgtcgctcat cttgggccac gccgcggcga atccctcgta
     1321 ggtggtccgg ccgagcagca tcgactccgc gtccatggtc tccttcagct tgaacgcata
     1381 tgagtccggg acgccgtacg ccatcgtcca gcccgcatgc gggtgcgtcg cctctccgcc
     1441 tggcgcctcg acgacgccat caagactgat gaactcggtg acgatcaggg tgcgcacggg
     1501 gatgtccttc cggttgttga gccacaggtg agccgtcggg gatatggacg ctacgacaac
     1561 ccagaactca tcggctgcgc tagcgtgccg agcgtgttca ccttggtggt tcgattcgac
     1621 ctgccggacg cggcgggcgc acgcgcgttc gacgagctga ctgcggccgc cgtgccgatc
     1681 attcgtgcgc acgaaccggg cacgctggtc tacgagacgt acgcagtcga aggcgcacca
     1741 ctggcccgcg tgttctacga ggtctatcgc gaccgcgatg cgcacgccga gcacgagcga
     1801 caaccgcgca ccgcggcgtt cctcaccgcg gtgcgcacgc tcgtcgccca gacccgcgtc
     1861 gagatcctgt cgcggctgga gtcaggcgga ctttaggcag ccggcgacga gggcagcgtt
     1921 acctgggtaa ccgttgctcc gggcggcagc tcgaacagcg atgcggacgg cgacgatgtg
     1981 tagctcttga tctcgaaact cgtgctgtca cccgccacct tgacgtaccc gaggatgccc
     2041 tgcgcggttg tgcagatcgt gctttcgccg gcctcacccg gcgtcgcgaa gtcgacgcac
     2101 gacatggcga acgcgttcac cgtcatggtt gacgccgtga ccttgtcccc ggcgaaaccc
     2161 gcagcaagcg agaacccctt caggaagttg atccagtggg cgggagtgta gaagtcgaag
     2221 attgcgttct gctcgtccgc ggcgaccggc gacaacatct ggcacgccca ggtcccctta
     2281 ccagccgagc cggatgtgtc gggcggggtg caggagtact cacctgtcga gttcacgatg
     2341 atgtcgaggc tcggtgcgtt ggtgtcgccc gtgctgcccg cgctgctggt gctcgtcggc
     2401 gtgttcgtaa acgagaggcc ctttggtggc tggacggcgt agacgaccgt cgtcggggtc
     2461 gacccagtgg tcacgtacgt ggcctcgaac ggcttcgctt caccggactg gatcttggtg
     2521 ctgaacgcgt ccatggtctt ctttttgttt tccacggcgt gcgcaaccga cttgaccttg
     2581 ttgatcgccg aacatccacc gagccctagg ccgccaagcg tgaccgcgag agcgacaact
     2641 ccggtcagcc ggtgacggcg aagtacgacg actgctcggt cggtgtctgg ggtcacgctg
     2701 tt
//
LOCUS       DATCBJ010000106         4689 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_393705, whole genome shotgun sequence.
ACCESSION   DATCBJ010000106 DATCBJ010000000
VERSION     DATCBJ010000106.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 4689)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4689)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4689
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_393705"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..246
                     /locus_tag="VII50_02190"
     CDS             <1..246
                     /locus_tag="VII50_02190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5049683.1"
                     /translation="DIADWRIAVVYLVGVPVAEAIPTPGGIGGVDAVLVAGLARVGTS
                     TAGAVIAAVIVFRLLTFWAPIVPGVISAGLLRRQHAL"
     gene            633..1571
                     /locus_tag="VII50_02195"
     CDS             633..1571
                     /locus_tag="VII50_02195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HEY5049684.1"
                     /translation="MLPADLLIADKRNNRLIVVDPQGRVVWQFPRPGDLAAGQTFLIP
                     DDAFFSADGKYIVATEEDDFAIRVVDVATHRIVASYGHPGVHGSGPGYVWNPDDAMML
                     ANGDLVAADIKNCRVIVVSTTAQTVTRQFGITGRCAHNPPSTFGSPNGAFPTTNGRYL
                     VTEINGDWVDEIDLTGHVYWSTHPPGVRYPSDTNEIGVNSYLTVDYSSPGQIVIFNQA
                     GQTLWRYAPTGSSQLNHPSLALPLPNGDIVVNDDLNDRVIVVDPRTNAVVWQYGHTGV
                     RGVAAGYLNIPDGVDLVPPYSFAAGHPAALSAPGGP"
     gene            1571..2077
                     /locus_tag="VII50_02200"
     CDS             1571..2077
                     /locus_tag="VII50_02200"
                     /inference="COORDINATES: protein motif:HMM:NF016121.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HEY5049685.1"
                     /translation="MKRAFAAIVATVAGLVLLLGFKTHSARLSAQPPIAATASSPPAP
                     QTSAPASAPPAGAPPASGPPAKTPSAAAPSAPAQTRTVTGSSIRTRYGDVEVQVTFTG
                     AQITDVVPVKLPDSNGVDQEIDQQVVPILIQETLTAQSANIQAVSGATYTSDGYIRSL
                     QSALDKAK"
     gene            2074..2934
                     /locus_tag="VII50_02205"
     CDS             2074..2934
                     /locus_tag="VII50_02205"
                     /inference="COORDINATES: protein motif:HMM:NF012471.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family zinc carboxypeptidase"
                     /protein_id="HEY5049686.1"
                     /translation="MSLRRGYPLWMLVVLALTGCAADRHADAVVVHVSSSGPAPMSPA
                     ASLLPRAAPIVASTLATAPPPPSPTPTPGLVNRTTTFGYSVRGRPLKVTEIGNPASTH
                     RVLIVGSIHGNELAGDQIATALQAAVPRADADLWIVQELNPDGAAAATRQNADSVDLN
                     RNFPFRWQPLGPRGSLNFSGTRALSEPESAAAASLIVRIHPTLAIWFHQHLAVVDDSE
                     GPINVERRFSALVALPLARLTDYPGSVAGWQDSMFGPTAFVVELPPGQLPSGSAKRYV
                     EAIEAVATMN"
     gene            complement(2958..>4689)
                     /locus_tag="VII50_02210"
     CDS             complement(2958..>4689)
                     /locus_tag="VII50_02210"
                     /inference="COORDINATES: protein motif:HMM:NF013507.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5049687.1"
                     /translation="AGGLDSRQVTTAQTLVFDPSSPSIRVAGRLAVAVHDAGAGVVGG
                     TALVVGGGSAASSDAVQAVRADGTSAIVGRLPQVRSDDSVAVASNELYVIGGYDGIHE
                     LPGVLATTDGATFHQVGQLGETVRYGAAYASLGAVWIFGGEHQGQPIADIQRVDTGTG
                     VSTVVGKLPHPLAHAAVAVLGGHVLILGGSDGHNPQNTIFAFDPATGSVSLVGSLPES
                     VSDMATAVVGSIAYVIGGNALTAEQTIAPTTAIVAVKVASVVAQATVQGSAAALFSGK
                     LLIADRGNNRLILVDPAGTIHWTFPNAAAPAPPTGFFFPDDAFFTEHGTAILSNQEGN
                     NTIVEISYPGGRPLWSYGHPGIASAAPGYLHEPDDAYKLADGRVIVADANNCRVLIIS
                     AQAQPIGQIGTTGTCIHKPPTSLGYPNGDTPLQNGNVLISEVTGSWISEYTTSGALVW
                     TIHLPLTYPSDPQQIGPDTYIVADYTKPGGIVEFDRAGHILWEYRVPSGHGMLDHPSL
                     AEVLPSGVICVNDDYRHRVVLIDPKTKTIVWQYGVDDVSGTAPGLLNTPDGFDLLAPD
                     GTTPTHPWTG"
BASE COUNT          785 a         1523 c         1511 g          870 t
ORIGIN      
        1 gacatcgccg attggcgaat cgccgtcgtc tatctggtgg gtgttcctgt tgcggaggcg
       61 attccgacgc caggcggaat cggaggcgtg gacgccgtgc tcgtcgctgg cctcgcccgc
      121 gtcggcacct ccaccgcagg cgcggtgata gctgcggtga tcgtctttcg attgttgacc
      181 ttctgggcgc cgatcgtgcc cggagtaatc tccgccggcc ttctgcggcg ccaacacgca
      241 ctgtgactgt tcccgtgccg aaccgacagc ttcatcgccg cacgctggtc gcgacggtgg
      301 tagcagcggc ggcggttgca gccgttgtgg tcggggcact gtcgggaacc tcgtcgtcct
      361 cgacgcccac gacgtcccat gcctcgggcg tccacaattt tccgctggct ggtccgactg
      421 cggcacccga tccaacagcg gcgtctgatc caactgcggc gccagctgca agcagttctg
      481 caccgcaatc tttcagcccg cgaattgatt cagcgccaac tgtgcctgcg gtctcatcgc
      541 ctacggtcgc cccaaccgtt gccgaaccgc catggacggc ggcggcgccc gtaggtgcgg
      601 cagggtacct gcagccaggg tctgacccga gcgtgttacc tgcggacctg ctgatcgcgg
      661 acaagagaaa caaccggctc atagtcgtcg acccccaggg gcgcgtggtg tggcagttcc
      721 cgcggcccgg cgaccttgcg gctggccaga cgttcctcat cccagacgac gcgttcttca
      781 gtgccgacgg caagtacatc gtggcaaccg aagaggatga ctttgcgatc cgcgtcgtcg
      841 atgtcgcgac ccaccggatt gtcgccagct atggacaccc gggcgtgcac ggttccgggc
      901 ccgggtacgt gtggaatcca gacgacgcaa tgatgctcgc gaacggcgac cttgttgccg
      961 ccgacatcaa gaactgccgg gtgatcgtcg tgtcgaccac ggctcagacg gttacgcggc
     1021 agttcgggat cactgggcgc tgcgcccaca acccgccatc caccttcggt agtcccaacg
     1081 gtgcgttccc gacaaccaac ggccgttact tggtcaccga gatcaatggc gattgggtcg
     1141 atgagatcga cctcactggt cacgtgtact ggtcgacaca ccctccgggc gtgcgatacc
     1201 cgtcggacac gaacgagatc ggcgtgaaca gctacctgac agttgactac tcatctccgg
     1261 gtcagattgt cattttcaac caagctggac agaccctttg gcgttatgcc ccgaccgggt
     1321 cgagccagct gaaccatcct tcgctcgcgc tgccgctgcc caacggcgac atcgtcgtca
     1381 acgatgacct caacgatcgc gtcatcgtcg tggacccgcg gacgaacgcc gtcgtctggc
     1441 agtacggcca cactggtgtg cgcggtgtcg cggccgggta tctcaacatt cccgacggtg
     1501 ttgatctcgt ccctccatat tcattcgcag ccggtcaccc ggctgcgctc agcgctcctg
     1561 gtggtccttg atgaagcgtg cattcgcggc gatcgtcgcc accgtggcag ggctggtact
     1621 gctgctcgga ttcaagacgc attcggctcg actgtcggcg caaccgccga tcgccgcaac
     1681 ggcgtccagc ccgccagcgc cacagacgtc ggcgcccgcg agtgcgccgc ccgcgggcgc
     1741 cccgcccgcg agtgggccgc ccgccaagac gccgtcagca gcggcaccct cagcgccagc
     1801 gcagactcgc accgttactg ggtcttcgat tcgtacgcgc tacggcgatg tcgaggttca
     1861 ggtgacattt acgggagccc agatcaccga cgttgttcct gtgaagctcc ccgacagcaa
     1921 cggcgtcgac caggaaatcg accagcaggt tgtcccgata ctgattcagg agacgctgac
     1981 ggcgcagagc gcgaatattc aggccgtgtc gggtgcgacc tacaccagcg acggctacat
     2041 caggtcgttg cagtcagcac tcgacaaggc gaagtgagtc tccgtcgcgg atacccgttg
     2101 tggatgctcg ttgtgctggc gctaaccggc tgtgcagccg atcgtcacgc tgacgcagtt
     2161 gtcgttcacg tgtcgtcgtc cggaccggcc cctatgtcgc cggccgcgtc gctgcttccg
     2221 cgggctgctc cgattgtcgc gagcaccttg gcgactgcac ctcctccacc atcgccgacg
     2281 ccgactcctg ggctggtcaa ccggacgaca acgttcggat attcggttcg tggtcggccc
     2341 ttaaaggtga ccgaaatcgg aaatccagcg agcacgcacc gggtccttat cgtcggcagt
     2401 attcatggaa acgagttggc cggcgaccag atcgcgaccg cattgcaagc tgctgtaccg
     2461 cgggcggacg cagacctgtg gatcgtccag gagctgaatc ccgacggcgc ggcggccgct
     2521 acgcggcaaa acgctgacag cgtcgacctc aaccggaact tcccgttccg atggcaaccg
     2581 ctaggcccac gcggctcgct caacttcagc ggaacgcgtg ccctgtccga gccggaatcc
     2641 gccgccgccg ccagtctgat tgtccgcatc cacccgacgc tggcaatttg gtttcaccag
     2701 catcttgctg tcgtcgacga ttccgaaggt ccaatcaacg tcgagcggcg cttcagtgcc
     2761 cttgtggccc ttccgcttgc acgactgacc gactaccccg ggagcgtcgc cggttggcaa
     2821 gactcgatgt tcggccccac ggccttcgtt gtcgagctgc cgcccgggca actgccttca
     2881 ggttctgcga agcgttacgt cgaggcgatc gaggccgtcg caacaatgaa ctaactgtcg
     2941 acagctgagt gtgtgcgtca gcctgtccat ggatgggtag gcgtggtgcc atcgggcgcg
     3001 agcaggtcga atccgtccgg cgtattcagc agcccaggcg cggtgccgga tacgtcgtcg
     3061 acgccgtact gccagacgat ggtcttggtt ttcgggtcga tcagtacaac gcgatgccgg
     3121 tagtcgtcgt tgacgcagat gacgccgctt ggcagtacct cggcgaggct cgggtggtcg
     3181 agcatcccgt gcccgcttgg gacgcggtac tcccacaaga tgtgcccagc tcggtcgaac
     3241 tcgacgattc cgccgggctt ggtgtagtcg gccacgatgt aggtgtcggg accgatctgc
     3301 tgtggatccg acgggtaggt cagtggcagg tggatcgtcc agaccagtgc gccggacgtg
     3361 gtgtactcgc tgatccacga gccggttacc tcggagatca acacgttgcc gttctgtagg
     3421 ggcgtatcgc cgttcgggta tcccagtgag gtcggtggct tgtggatgca ggtgcctgtg
     3481 gtcccgatct ggccgatggg ttgcgcctgc gcgctgatga tcaagacccg acagttgttg
     3541 gcgtcggcga cgatgacgcg cccgtcagcg agcttgtagg cgtcgtccgg ttcgtgcagg
     3601 tagcccgggg cagcgctcgc gatgcccggg tggccgtaag accagagcgg ccttccgccg
     3661 ggataggaga tttcgacgat cgtgttgttc ccctcctggt tcgacaggat cgccgtgccg
     3721 tgttccgtga agaaggcatc gtcaggaaag aagaatccgg tcggcggcgc gggagcggcc
     3781 gcattcggga aggtccagtg gatggtgccc gccgggtcaa caaggatgag gcggttattg
     3841 ccgcggtcgg caatgaggag cttgccgctg aacagcgcgg ccgcggagcc ctggaccgtc
     3901 gcctgcgcga ctaccgatgc gaccttcacg gcgacaatgg cggttgtcgg cgcgattgtc
     3961 tgctccgcag tcagggcgtt tccaccgatg acataggcga tgctgccgac gacagccgtt
     4021 gccatgtcgg aaaccgactc cggcaaggag ccgacgaggc tgaccgagcc ggtggccggg
     4081 tcgaaggcga agatcgtgtt ttgcgggttg tgcccgtcgg atccgccgag gatcaagacg
     4141 tgaccgccga gtacggccac cgcggcatgc gccagcgggt gcgggagctt gccgacaacc
     4201 gtgctcacgc cggtgccggt gtcgacgcgt tggatgtcgg caatgggttg gccctggtgc
     4261 tcgccgccga agatccatac cgcgcccaat gacgcgtacg cagcgccgta acgcacggtc
     4321 tcacctagct gtccgacctg atgaaaggtg gcgccgtcgg tggtggcgag aacacccgga
     4381 agttcgtgga tgccgtcgta cccaccgatc acgtacaact cgttgctcgc caccgcgacg
     4441 gaatcgtccg agcgaacttg agggagccgt ccgacaatcg ccgacgtgcc atcggcccgc
     4501 actgcttgca ctgcatcgct cgatgcggcc gagccgcctc cgaccacgag tgccgtgcct
     4561 ccgacgactc ctgccccggc atcatgcacc gcgaccgcga gacggccggc cacccgaatc
     4621 gacggtgagc tgggatcgaa gaccagggtt tgggcggtcg tgacttgccg cgagtcgagt
     4681 ccgccagcc
//
LOCUS       DATCBJ010000107         3539 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_251632, whole genome shotgun sequence.
ACCESSION   DATCBJ010000107 DATCBJ010000000
VERSION     DATCBJ010000107.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 3539)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3539)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3539
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_251632"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1309
                     /locus_tag="VII50_02215"
     CDS             <1..1309
                     /locus_tag="VII50_02215"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY5049688.1"
                     /translation="ARMHRLRVKVFGDLPNEVAVLSAVAFSVAVGFGIVAPVIPLFAH
                     HFGVGKAEVGAVLSAFAFMRLASALGVGRLVDAIGERVVLATGIGIVAVSSALAGFAH
                     SYLQLLLLRGAGGIGSAMFSVSAMGLLLRVVGPAQRGRSVGLFQGGFLLGGISGPAIG
                     GPLAAWSIRAPFFVYAGTLVVAGTVAMVGLRRASLARKTEQVDADQPSRTSLAAAFKQ
                     RAYRAALVANLADSWAAMGVRNTLVPLFVVESLHRGTAWVGIGFTIVAFFNAATLIPA
                     GRLVDSRGRRPVMVGGCLLSASSMALLALSPNLVGYIIAMVVFGIGSGLLDVAPAAIV
                     GDVAGKRGGTVVAGFQMAGDGGSVSGPIVAGWVADTWSYGAAFWLTTGILAGAGALAA
                     RAPDIRAANGEPAPDQPASRRRFRSLPTVEPGPPVLQGDSPPR"
     gene            complement(1200..2366)
                     /locus_tag="VII50_02220"
     CDS             complement(1200..2366)
                     /locus_tag="VII50_02220"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009083187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HEY5049689.1"
                     /translation="MTDHSDDMHSTESFETRAIHAGQPSDPRTGAVVPPIFQVSTFHQ
                     DAVGSPRHGWEYSRSGNPTRAALEACLADLDGGARGLAFASGLAAEDCILRTLCAPHD
                     HVVVPGDAYGGTFRLVDKVFGRWGVTHTSVDLSDLDWLVRAMTPKTTVVWCETPSNPL
                     LGIADIAAVARIAHEGGAKLVVDNTFASPYLQQPLALGADIVVYSTTKYLGGHSDVVG
                     GALVAADAELGDQLAFHQNAIGSIAGPFDSWLVMRGVKTLAVRMDRHCENAARIAAFL
                     QHHKAVSQVLYPGLPTHAGHEIAAKQMRDFGGMVSFRMDAGEAAAVDVCNRARLFTLA
                     ESLGGVESLIEHPGRMTHVSVVGSPLEVPADLVRLSVGIENVDDLLADLEQALR"
     gene            2482..3177
                     /locus_tag="VII50_02225"
     CDS             2482..3177
                     /locus_tag="VII50_02225"
                     /inference="COORDINATES: protein motif:HMM:NF013117.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HEY5049690.1"
                     /translation="MAWRERPVAAAAFADARELIFRPKFRRALVTAVVFVAAAIAGAY
                     DNGVKARHTVNFVLVPDQPHRVFALVAIGVCLVAGIITVQLTANELARLARHRGSPAA
                     ASAIRLSVQIVGYLAILVTVLGLFAVKIESVLLSGAIVSVVLGLAAQQSLGNAFAGIV
                     LLISRPFKVGDYITLRAGALGGQYDGQISAISLMFTTMRTKEGPISFPNAAVLSSATG
                     VREDPSGSSPLIT"
     gene            complement(3190..>3539)
                     /locus_tag="VII50_02230"
     CDS             complement(3190..>3539)
                     /locus_tag="VII50_02230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5049691.1"
                     /translation="KLLRPGGWMVFDDLPWTYASSPALSQLPWVQKMSDEERTTPQVQ
                     RIFDLLVKTHPAYDLFREEGQWGLAQKALTTSGTGAEVRIVKEVEVRREKYGLGAVAV
                     KAARKASRKLKRP"
BASE COUNT          527 a         1222 c         1149 g          641 t
ORIGIN      
        1 ggcgcggatg caccggctac gtgtgaaggt gttcggcgac ctgccgaacg aggttgccgt
       61 cctgtcggcg gtggcgttca gcgtggcggt cgggttcggc atcgtcgcgc ccgtcatccc
      121 actgttcgcg catcacttcg gtgtcggaaa ggcggaagtc ggcgccgtcc tgtctgcctt
      181 cgcgttcatg cgactcgcgt ccgcactcgg cgttggccgc ctggtcgacg ccatcggtga
      241 acgcgtggtg ctggccaccg gcatcggcat cgttgcggtc agcagtgcac tcgccggatt
      301 cgcgcacagc tacctgcaac tgctgctgct gcgcggcgct ggtggtatcg gttccgcgat
      361 gttctcggtg agcgcgatgg gactgttgct gcgcgtcgtc ggcccagcac agcgcggtcg
      421 ttcggtcgga ctcttccagg gcggcttcct gctcggcggc atctctggcc cggccatcgg
      481 cggaccgctc gccgcgtggt cgatccgcgc accgttcttc gtctacgccg gtacgctcgt
      541 cgttgccggc accgtcgcga tggtcggcct gcgacgtgcg tctctcgccc gcaaaaccga
      601 acaagtggac gccgatcagc ccagccgcac gagcctcgcc gcggccttca agcaacgggc
      661 ctatcgagcg gcgctggtgg ccaacctcgc cgacagctgg gccgcaatgg gagtgcggaa
      721 cactcttgtc ccgctgttcg tggtcgagtc cctgcatcgc ggcaccgcct gggtcggcat
      781 cggattcacc atcgtggcgt tcttcaacgc ggccaccctg attcccgcgg gccgtctcgt
      841 cgactcgcgt ggccggcgtc ccgtgatggt ggggggctgc ctgctgtcgg ccagctcgat
      901 ggcgttgctc gcgttgtcgc cgaacctcgt cgggtacatc atcgcgatgg tcgtattcgg
      961 gatcggttcg ggattgctcg acgtcgcacc ggccgcgatc gtcggcgatg tcgcgggcaa
     1021 gcgcggcggc acggtggtcg ccggatttca gatggccggc gacggcggtt cggtaagcgg
     1081 cccgatcgtg gccggctggg tggccgacac ctggtcctac ggcgccgcgt tttggctgac
     1141 gaccggcatc ctcgcgggcg ccggcgccct agccgcccgc gcgcccgaca ttcgagcggc
     1201 taacggagag cctgctccag atcagccagc aagtcgtcga cgttttcgat ccctaccgac
     1261 agtcgaacca ggtccgccgg tacttcaagg ggactcccca ccacgctgac atgcgtcatc
     1321 cgccccggat gctcgatcag cgactcgacc ccaccgagtg actcagccaa cgtgaacaat
     1381 ctcgcgcggt tgcaaacgtc gacggcggcc gcttccccgg cgtccatccg aaacgacacc
     1441 atgcccccga agtcgcgcat ctgtttcgcg gcaatctcgt gaccggcatg cgtgggcagt
     1501 cccgggtaca gcacctgcga aacagccttg tggtgttgca ggaatgccgc gatgcgggcc
     1561 gcgttttcgc agtgccgatc catgcggacg gcgagtgtct tcacgccgcg catcaccagc
     1621 cacgaatcga acggaccggc gatagagccg atggcgttct ggtggaacgc gagttggtca
     1681 ccgagctcgg catcggccgc gaccaatgcg ccgcccacga catcggagtg gccgccgaga
     1741 tacttcgtcg tggaatacac gacgatgtcg gcgcccaaag ccagcggttg ctgcaaatac
     1801 ggcgacgcga aggtgttgtc aacgaccagc tttgcgccgc cctcgtgtgc gatccgggcg
     1861 accgccgcga tgtcggcgat ccccagtagc ggattgctcg gcgtctcgca ccacaccacc
     1921 gtcgtctttg gcgtcatcgc gcggaccagc cagtcgaggt cggacaggtc gaccgacgtg
     1981 tgcgtcacgc cccaccggcc gaacaccttg tcgaccaacc ggaacgtgcc gccgtacgca
     2041 tcgccgggca cgacgacgtg gtcatgcggc gcgcacaacg tgcgcaggat gcagtcctcg
     2101 gccgccaaac cgctcgcgaa agccaacccg cgcgcgccgc cgtcgagatc ggcaaggcac
     2161 gcctcgagcg ccgcccgcgt cggattgccc gaccggctgt actcccaccc gtggcgcgga
     2221 ctgcccaccg cgtcttggtg gaacgtcgag acttgaaaga tcggggggac gacggcacct
     2281 gtgcgtgggt ccgacggctg gccggcgtga atggcccggg tctcgaagct ttccgtggag
     2341 tgcatgtcat cggagtggtc tgtcatgaag ccgaggctat cccgcagccg ctaagttggc
     2401 cgtcaatatg gcgcgcaggc gccgtcccgc accaccctgc tggcccgcat cacccgccgt
     2461 ccggcatcag aggaggtcgt tgtggcctgg cgcgagcgac cagtcgcggc cgctgccttc
     2521 gccgatgccc gcgagctgat cttccgcccc aagttccggc gcgcactcgt caccgcggtg
     2581 gtgttcgttg ccgcggccat cgcgggggcc tacgacaacg gcgtcaaagc caggcacacc
     2641 gtgaacttcg tactcgtgcc tgatcagccg caccgagtct tcgccctggt ggcgatcggc
     2701 gtgtgcctgg tcgccggcat catcaccgtc caactaacgg ccaacgaact cgcgaggctg
     2761 gcgcggcacc gtggcagccc cgccgctgcg tccgccatcc ggctgagcgt gcagatcgtc
     2821 gggtatctgg cgattctcgt gacggtgctc gggttgttcg cggtcaagat cgagagcgtt
     2881 ctgctctccg gcgccatcgt cagcgtcgtc cttggactgg cagcgcagca gtcgctcggc
     2941 aacgcgttcg cgggcatcgt gttgctcatc tcacgaccgt tcaaggtcgg cgactacatc
     3001 acgttgcggg ccggcgcgct gggcgggcag tacgacggcc aaatcagcgc gatcagtttg
     3061 atgttcacca cgatgcggac gaaggaagga ccgatttcct tcccgaatgc ggcggtgctg
     3121 tcctctgcga ccggggtgcg cgaggatccc agcggcagct cgccgctgat cacctaatgt
     3181 cctgaccgcc taggggcgct tcagctttcg gctggccttg cgcgctgcct tcaccgcgac
     3241 tgcacccagg ccgtacttct cccgacgcac ttccacttcc ttgacaatcc tgacttccgc
     3301 cccagtgccg gacgtggtca gcgccttctg cgccaggccc cattggcctt cctcgcggaa
     3361 caagtcgtac gcggggtgcg tcttcaccag cagatcaaag attcgctgga cctgcggggt
     3421 cgtacgctcc tcgtcggaca tcttctgcac ccatggcagc tggctgagag ccggactact
     3481 ggcatacgtc cacggcaggt cgtcgaacac catccatcca cccgggcgca gcagcttgt
//
LOCUS       DATCBJ010000108         4462 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_320600, whole genome shotgun sequence.
ACCESSION   DATCBJ010000108 DATCBJ010000000
VERSION     DATCBJ010000108.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 4462)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4462)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4462
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_320600"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..768)
                     /locus_tag="VII50_02235"
     CDS             complement(<1..768)
                     /locus_tag="VII50_02235"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009073872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003985 - acetyl-CoA C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HEY5049692.1"
                     /translation="MTSTAHAPSEICDALPAVIVPSGENAGRNRASDSMAFDGLTDPF
                     EHIAMGESTERYNAHLGISREEQDAFAARSHQRAAVARKEGLFDDEIVAVPVPHRRGE
                     PVMVAQDEGIREDTTIESLARLRPAFSPDGTITAGSASQISDGACAVLVTSRENAGRR
                     GWPILAEIGAHGVVAGPDNSLHSQPSHAIARALAREQLAPAEIDLFEINEAFAAVAIQ
                     SMRELDIDPSVVNVNGGAIALGHPIGMSGARLAVHLIY"
     gene            683..1177
                     /locus_tag="VII50_02240"
     CDS             683..1177
                     /locus_tag="VII50_02240"
                     /inference="COORDINATES: protein motif:HMM:NF014820.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5049693.1"
                     /translation="MRPAFSPDGTITAGSASQISDGACAVLVTSRENAGRRGWPILAE
                     IGAHGVVAGPDNSLHSQPSHAIARALAREQLAPAEIDLFEINEAFAAVAIQSMRELDI
                     DPSVVNVNGGAIALGHPIGMSGARLALHLIYELRRRGGGIGVAGLCGGGGQGDALVLR
                     VAAH"
     gene            1198..2145
                     /gene="meaB"
                     /locus_tag="VII50_02245"
     CDS             1198..2145
                     /gene="meaB"
                     /locus_tag="VII50_02245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0015819 - lysine transport [Evidence IEA];
                     GO_process: GO:0015822 - ornithine transport [Evidence
                     IEA];
                     GO_process: GO:1903826 - L-arginine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HEY5049694.1"
                     /translation="MTSDIEGLVKAARAHDARALGKLLSMVEDDDPALPALMSELRGA
                     GVHAAYVIGLTGAPGVGKSTTTSALIALWREREQRVGVLAVDPTSPFTGGALLGDRVR
                     MQTHATDSDVFIRSMASRGQLGGLAASTPQAVRVLEATGFDLVLVETVGVGQAEVDIA
                     ATADTTVVLLAPGMGDGIQAAKAGLLEVADLLVVNKCDRDGADVAVRELQNMIATGGE
                     REPGEWRPVVVRATATAAGGIDELVDAVDKHRAWLVSSGDGEQRRVRRATQEIRSIAL
                     AMLSARVGLRADDPRLAELAGAVAAGTIDAYTAAGRLVS"
     gene            complement(2138..2905)
                     /locus_tag="VII50_02250"
     CDS             complement(2138..2905)
                     /locus_tag="VII50_02250"
                     /inference="COORDINATES: protein motif:HMM:NF021425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2127 domain-containing protein"
                     /protein_id="HEY5049695.1"
                     /translation="MDWSLRTCGRRGHVTYAPTEPGLRERLHVVTPVGEAWRCLRCGD
                     FVLGAPVGDGPAGDAPTVLRGRALRDAFVLRLLAAERFIRALVIGAGSYAVLRYSNSE
                     NSLRALFEKDLPAAKPLANAFGYNLDNSSIVREIRKLLLVKPSTLHLVAILLAVYAVI
                     ELCEAVGLWLLKRWGEYFAAVATGIFLPYEIYDIVNKVTAIRVGTFIINVAAVVYLLW
                     TKRLFGIRGGYAAYEAKRHEESLLEVEEAARPEAEVS"
     gene            2955..3464
                     /locus_tag="VII50_02255"
     CDS             2955..3464
                     /locus_tag="VII50_02255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009073908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEY5049696.1"
                     /translation="MGKPLNLPFDPIERAAQRWEETWGPASAMRVATSIMRAQQILLA
                     RYDELLKPHGLTFARYEVLVLLRFSRYGALPLGRIGERLMVHPTSVTNSIDRLEAAGF
                     VERLPNPRDGRGTLAAITESGLAVVEAATADLMAADFGLDALAEPARAEVVDALRTLR
                     LDAGDFSTE"
     gene            3524..>4462
                     /locus_tag="VII50_02260"
     CDS             3524..>4462
                     /locus_tag="VII50_02260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HEY5049697.1"
                     /translation="MDARHSADRQGMAAARGSADGRPRSTLGGLPLEPAYGPPDGADD
                     PRVARIGRPGEYPFTRGLYTSGYRGRPWTIRQFAGFGNATQTNERYRMILDAGGGGLS
                     VAFDMPTLMGRDSDDPQARGEVGHCGVAIDSASDMDLLFQGIPLGDVTTSMTISGPAV
                     PVFCMYVAAAERQGVAHGKLNGTLQTDIFKEYMAQKEWLFTPQPHLRLIGDLMEFCAH
                     EMPDYKPLSVSGYHIREAGSTAVQELAYTLANGFAYVELGLSRHIDVDTFAPQLSFFF
                     DAHIDFFEEIAKFRAARRIWAHWMRDVYGAKTERAQW"
BASE COUNT          734 a         1434 c         1545 g          749 t
ORIGIN      
        1 atagatcaga tgcacagcga ggcgtgctcc tgacatcccg atcgggtgac cgagcgcgat
       61 cgcgccgcca ttgacgttga ccacgctcgg gtcgatgtcg agttctcgca tcgactggat
      121 cgccaccgcg gcgaatgcct cgttgatctc gaacagatcg atttctgcgg gcgccagttg
      181 ttcgcgtgcc agcgcccgcg cgatggcgtg cgacggttgt gagtgcagcg agttgtctgg
      241 tccggcgact accccgtgcg caccgatctc cgcgaggatc ggccagcctc gtcgtccggc
      301 attctctctt gacgtgacca gcaccgcgca cgcaccgtcg gagatctgcg atgcgctgcc
      361 cgcggtgatc gtgccgtccg gtgagaacgc cggccgcaac cgtgcgagcg actcgatcgt
      421 ggtgtcttcg cgaatgccct cgtcttgggc gaccatgacc ggctcgccgc ggcggtgcgg
      481 cacgggcacg gcgacgatct cgtcgtcgaa cagaccttcc ttgcgcgcta ccgctgcgcg
      541 ttgatgcgag cgggccgcga acgcgtcctg ttcctcgcga ctgataccga gatgcgcgtt
      601 gtaacgttcg gtcgactcgc ccatcgcgat gtgttcgaac gggtcggtca ggccgtcgaa
      661 cgccatcgag tcgctcgcgc ggttgcggcc ggcgttctca ccggacggca cgatcacggc
      721 gggcagcgcg tcgcaaatct ccgacggtgc atgcgcggta ctggtcacgt caagagagaa
      781 tgccggacgt cgagggtggc cgatcctcgc ggagatcggt gcgcacgggg tggtcgccgg
      841 gcccgacaac tcgctgcact cgcaaccgtc gcacgcgatc gcacgggcgc ttgcacgcga
      901 acaactcgcg cccgcagaaa tcgatctgtt cgagatcaac gaggcgttcg ccgcggtcgc
      961 gatccagtcg atgcgagaac tcgacatcga cccgagcgtg gtcaacgtca atggcggcgc
     1021 gatcgcgctg gggcacccga tcgggatgtc aggggcacgc ctggccctgc atctgatcta
     1081 tgagcttcgc cgccgcggtg gaggcattgg cgtcgccggt ctttgcggcg gtggcgggca
     1141 gggtgacgcg ctggtcctgc gcgtcgcggc gcattaaatc caacacggct aaacgcaatg
     1201 acgtcggaca tcgaggggct ggtgaaggct gcacgggcgc acgatgcccg tgctctggga
     1261 aagctgctct cgatggtcga ggacgacgac cccgccctgc ccgccctcat gtcggaactt
     1321 cgcggggccg gcgttcacgc ggcctacgtc atcgggctca ctggcgcgcc gggtgtcggt
     1381 aagtccacga cgacgtccgc gctgatagcc ctgtggcgtg aacgcgagca acgagttggt
     1441 gtgcttgcgg tcgatccgac atcgccgttc acgggtgggg cgttgctggg cgatcgggtg
     1501 cgcatgcaga cccatgccac cgactcggac gtcttcatca gatcgatggc ctcgcgcggg
     1561 caactcggcg gcctggcggc gtcgacgccg caggcggtgc gtgttctcga ggcgaccggg
     1621 ttcgacctcg tcctcgtcga gaccgttggg gtgggccagg ccgaggtcga catcgcggcg
     1681 acggccgaca ccacagtcgt gctactcgcc ccgggcatgg gcgacggcat tcaggcggcg
     1741 aaggccgggc tgctcgaggt cgccgatctg ctggtggtca acaagtgcga tagagacggg
     1801 gcggacgtcg cggtgcgcga gttgcagaac atgatcgcga cgggtggcga gcgagaaccg
     1861 ggggagtggc gacctgtcgt ggtgcgcgcg accgccaccg ccgctggtgg gatcgacgag
     1921 ctggttgacg cggtcgacaa gcatcgcgca tggctcgtgt catcaggtga tggcgagcag
     1981 cggagggtcc gccgcgcgac gcaggaaatc aggtcgatcg ccctcgcgat gctgtcggca
     2041 cgagtcgggt tacgcgccga cgacccgcga cttgccgagc tggctggcgc cgtcgccgcc
     2101 ggcacgatcg atgcctacac agctgccggt cggctcgtca gctgacttca gcctccgggc
     2161 gcgcggcctc ttcgacctcg agtagcgact cttcatggcg cttcgcttcg taggctgcgt
     2221 agccgccgcg gatcccgaac agtctcttgg tccataacag atagacgacg gcggcgacgt
     2281 tgataatgaa cgtgccgacc ctgatcgcgg tcactttgtt aacgatgtcg tagatctcgt
     2341 acggcaagaa gatgccggtg gccaccgcgg cgaagtattc gccccatcgc ttcaacagcc
     2401 agaggccgac cgcttcgcac aactcgatga ccgcgtagac cgccagcagt attgcaacga
     2461 ggtgcagtgt cgagggcttg acgagcagca acttgcggat ctcgcggacg attgaggagt
     2521 tatcgaggtt gtagccaaag gcattggcga gcggcttcgc ggcgggcagg tccttttcga
     2581 agagcgcgcg cagcgagttc tcgctgttgg agtagcgcag gacggcgtac gagccggcgc
     2641 cgatgacaag cgcacgaatg aaccgctcgg ccgccagcaa ccgcaacacg aacgcgtcac
     2701 gcaacgcgcg gccgcgcagg accgtcggcg catctccagc cggaccgtct ccaaccggtg
     2761 cgccgaggac gaagtcgccg cagcgcaggc accgccatgc ctcgccgacg ggggtcacga
     2821 cgtgcagccg ttcgcgcagc cccggctcag tcggggcata cgtgacgtgc ccgcgccgac
     2881 cgcaggttcg caggctccag tccacgagct gaagcgtagg ggaactgcag ccccgtacgc
     2941 ggtaggttgc gcacgtgggg aaaccgttga atctgccgtt cgatccgatc gagcgggcgg
     3001 cgcaacgctg ggaagaaacg tggggcccgg cctcggccat gcgggtggcc acctcgatca
     3061 tgcgcgcgca gcagatcctg ctggcccgct atgacgagct gctcaagcct cacggcctta
     3121 cttttgcccg ctacgaggta ctcgtgctgc tgcgtttctc gcggtacggc gcgcttccgc
     3181 tcggccggat cggcgagcgg ctgatggtgc acccgacgag cgtcacgaac tcgatcgacc
     3241 gtctcgaggc ggccggcttc gtcgaacgtt tgcccaaccc gcgggacggc cggggcacgt
     3301 tggcggcgat caccgagagc gggctggccg tcgtcgaggc tgccaccgcc gatctgatgg
     3361 cggccgactt cggactcgac gcacttgccg agccggcgcg agccgaggtg gtcgacgccc
     3421 tgcgcacctt gcggctggat gcaggcgact tcagcacgga gtagcgaacg ccgaagaata
     3481 gtcggtcgtc ctactatcga aggcgaagct gctggaggca ggcgtggacg cacggcacag
     3541 cgcggatcgt cagggcatgg ccgcggcacg aggcagcgcg gacggccggc cacgatcgac
     3601 gctcggcggg ctaccactgg agcccgcgta cgggccgccc gacggcgccg acgatccgcg
     3661 ggtcgcccgg atcgggcgcc ccggcgagta tcccttcacc cggggcctgt acaccagcgg
     3721 gtatcgcgga cgaccgtgga cgatccgcca gtttgccggc ttcggcaacg cgacgcagac
     3781 caacgagcgg taccgcatga ttctggacgc cgggggtggc ggtctgtcag tcgccttcga
     3841 catgcccacg ctcatgggtc gcgattccga tgatccgcag gctcgtggcg aggtcggcca
     3901 ttgcggcgtt gccatcgact cggcgtcgga catggatctg ctattccagg gcattccgct
     3961 cggcgatgtc acgacgtcga tgacgatcag cgggcccgcc gtgccggtgt tctgcatgta
     4021 tgtcgccgcc gccgaacgcc aaggcgtcgc gcacggcaag ctgaacggca cgctgcagac
     4081 cgacatcttc aaggagtaca tggcgcagaa ggagtggctg ttcacgccgc agccgcatct
     4141 gcgtctcatc ggtgacttga tggagttctg cgcgcacgag atgccggact acaagccgct
     4201 gtcggtgtcg ggctaccaca tcagggaagc cggctcgacg gccgtgcaag agctcgcgta
     4261 cacactcgcc aacgggttcg cgtacgtcga acttggtctg tcgcggcaca tcgacgttga
     4321 cacgttcgcg cctcagttgt ccttcttttt cgatgcccac atcgacttct ttgaagagat
     4381 cgcgaagttc cgggcggccc ggcggatctg ggcgcactgg atgcgtgacg tctatggagc
     4441 gaagacagaa cgcgcgcagt gg
//
LOCUS       DATCBJ010000109         2199 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_102911, whole genome shotgun sequence.
ACCESSION   DATCBJ010000109 DATCBJ010000000
VERSION     DATCBJ010000109.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2199)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2199)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2199
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_102911"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            107..>2199
                     /locus_tag="VII50_02265"
     CDS             107..>2199
                     /locus_tag="VII50_02265"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HEY5049698.1"
                     /translation="MTVSAPPRPVGSASGIAVAASAPAVDVLRELGVAAGVGLSSAEV
                     AQRQAQYGPNSVSSHRARLLPVLWHQLRSPLLALLLIAAVASYFVGERSDAIIIGVIV
                     GLSVGLGFVNEYRAERAAEALHDQIHHETIVIRDGQPASVDVGALVPGDLVELRLGSI
                     VPADIRLIEVTNFECDESILTGESLPVEKGGGQVAAGTALAELTGCALMGTVVHAGSA
                     AGVVVTTGARTEFGKIAAGLGTHQLDTEFQVGLRKFSMLLVYVAGALTTSIFVINVVL
                     HKPIIDAALFSLAIAVGITPQLLPAVVSTSLAAGSRRMSHRKVLVKRLVCIEDLGNVD
                     VLFTDKTGTLTLGRIDYMRAVPAVSGASDDEVVRWGLLSTETTGTDSPAVGGNPLDSA
                     LWESPAAANERASLAGYTRVGLLPFDHERRMVSAIVRDAEGQFTLVTKGAPESVFDRC
                     IDVPASARDALAAEFVAGNRVVAVASRLVAEAAQPTPDDERGLSLVGLLVFLDPPKDG
                     AADALRRLANLGIAVKVVTGDNAAVAVKVCNELGLDDGDALTGADLDALDDAALAAAI
                     GRTTVFARVSPEDKARIVRVQRSTGGAVAFLGDGVNDALALHAADVGISVESATDVAK
                     DAADVILLEKDLNVLADGVAEGRRIFANTIKYVLMGTSSNFGNMFSAAGASLFLKFLP
                     MLPSQILLNNMLYDS"
BASE COUNT          346 a          682 c          744 g          427 t
ORIGIN      
        1 cgcccatgac ctggctcctt accgacggct agctttcagt tcattgagac tgggtacatg
       61 agccgggaag tagttacggg tcgaccatga cgggcgggcg ccgaccatga cggtttctgc
      121 gccgccgcga ccggtgggct ccgcgagcgg tattgcggtc gccgcctccg cgcccgcggt
      181 cgatgtcctg cgagagctcg gtgtcgcggc gggtgtgggt ctgtcgagcg ccgaggtcgc
      241 gcaacggcaa gcgcagtacg ggcccaactc ggtgtcgtcc catcgtgctc gcttgctgcc
      301 tgtcctgtgg catcagttgc gatcgccgct cctggccttg ctgctcatcg ctgccgtcgc
      361 ctcgtatttc gtaggtgagc gaagcgacgc catcatcatt ggcgtcattg tcggcttgtc
      421 cgtggggctc ggcttcgtta acgaatatcg ggccgagcgc gcagcggagg cactgcacga
      481 tcagattcac cacgagacca tcgtcatccg cgatgggcag ccggcgtccg tcgatgttgg
      541 agctctggta ccgggcgatc tggtcgagct gcggctcggc agcatcgtgc ccgcagacat
      601 ccgcctcatc gaggtcacca acttcgagtg cgacgagtcg attctgaccg gtgaatcgct
      661 gccggtggaa aagggtggcg gccaggtggc ggccggaact gctctggctg agctcactgg
      721 gtgcgcgctc atgggcactg tcgtgcatgc gggcagcgca gctggggtcg tggtcacgac
      781 cggcgctcgc acggagttcg gcaagatcgc ggctggtctc ggcacccacc aactcgacac
      841 ggagttccaa gttgggctac gcaagttttc gatgttgctc gtctacgtcg caggtgcgct
      901 gacgacgtcg atatttgtca tcaatgtcgt gctgcacaag ccgattatcg acgcggccct
      961 cttctcgttg gcgatcgcgg taggtatcac gccccaactc ttgcccgcgg ttgtgtcgac
     1021 cagcctggca gcggggtcgc ggcggatgag ccatcgcaag gtgttggtca agcggctggt
     1081 ttgcatcgag gacctcggca atgtcgacgt gctcttcacc gacaagacgg gcacgttgac
     1141 gcttggccga atcgactaca tgcgcgctgt gcctgccgtg agtggcgcca gtgacgacga
     1201 ggtagtgcga tgggggctgc tgtccaccga gaccacaggc accgacagtc ccgcagtcgg
     1261 cggaaacccg ctcgactccg cgctgtggga atcgcctgcc gctgcaaacg aacgagcctc
     1321 gctcgcgggc tacacgcgcg tcggactgct gccgttcgac catgagcggc ggatggtctc
     1381 ggcgatcgtt cgggacgccg aggggcagtt cacgctcgtc accaaaggcg ccccggagag
     1441 cgtgttcgac cgctgtatcg acgtcccggc ttccgctcgc gacgcactag cggccgagtt
     1501 cgtcgcgggc aaccgggtcg ttgcggtggc ttcccgcctc gttgcggaag cagcgcaacc
     1561 tacgccggac gacgagcgcg gattgtctct tgtcggcttg ctggtcttcc tcgacccacc
     1621 gaaggacggt gcagccgacg cgttgcgtcg actggcgaac ctcggcattg cggtgaaagt
     1681 tgtgaccggc gataacgcgg cggtcgcggt caaggtgtgc aacgagctcg ggctcgatga
     1741 cggggacgcg ctcacgggcg ccgatctcga tgcgctggac gacgctgcgt tggccgccgc
     1801 gattggccgc accaccgtgt tcgcccgggt cagccccgag gacaaggcgc gcattgtgcg
     1861 cgttcaacgg agcaccggtg gagcggtcgc gtttctcggt gacggggtga acgacgccct
     1921 cgctctgcac gccgcggacg ttggcatctc ggtcgagtcg gcgaccgacg ttgccaagga
     1981 cgctgccgat gtgatcctgc tcgagaaaga cctcaatgtg ctcgccgacg gagtggctga
     2041 ggggcgacgg atcttcgcca acacgatcaa gtacgtgctg atgggcacgt cgagcaactt
     2101 cggaaacatg ttttctgcgg ctggggcgtc gttgttcctg aagttccttc cgatgttgcc
     2161 gtcccagatc ctgctcaaca acatgctcta cgacagcag
//
LOCUS       DATCBJ010000110         2107 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_394911, whole genome shotgun sequence.
ACCESSION   DATCBJ010000110 DATCBJ010000000
VERSION     DATCBJ010000110.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2107)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2107)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2107
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_394911"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..246)
                     /locus_tag="VII50_02270"
     CDS             complement(<1..246)
                     /locus_tag="VII50_02270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5049699.1"
                     /translation="MEGDAQAGVALPGVVLPPVVAVVDRATAVAEEARPVRIGRPAAV
                     AGSRCASGRCATPLVLDRLAEEQRRAVRLVEECGGAGA"
     gene            312..1997
                     /locus_tag="VII50_02275"
     CDS             312..1997
                     /locus_tag="VII50_02275"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HEY5049700.1"
                     /translation="MIGFVMIVFTGRLLQLQGVDSTVYAADANAQYSHKVTLPAQRGA
                     IVDRNGATLADTVNASDVIGDPLVATNSTTTSPWTMAATLSPILGVNRFTLVSDLTGK
                     SQFVYLAHAVTPQIAKQVLALDLPGVTTENTNKREYPAGNVAANVVGFVGSDGKGLGG
                     LEYQYQNLLAGKEGQLTVQVGHDGQVIPDGVGGGQAAVPGQGIELTIDRDIQWKAQQA
                     IAAQVQATGADGGSVIVMQPRTGQILAMATTPTFDPADPGAAPAEDRGNSALSDVFEP
                     GSTNKVITMSAAIDSGVLTPMSPIDVPPTLVRAGKVFHDAESHGLLHLTLAGVLAESS
                     NIGTILTSEKVGVDRLYAYLQAFGLGQPSGLNFPGESPGILDPLRNWSATQQYTVPYG
                     QGLAVNALQVATVYSTIANGGVRVEPSLVKGFLQPDGSVKPANAPAQTRVVSPSTATQ
                     VEGMLEAVTTDQGTAPEARIPGYRVAGKTGTAQRVDPTCGCYRGYTASFVGFAPADNP
                     QLVVLVVLDNPIHGYYGGAVAAPVFKDVMSFALESEKIPPTGTTPPVVKLSLP"
BASE COUNT          362 a          715 c          686 g          344 t
ORIGIN      
        1 cgcaccagcg cccccgcact cctcgaccag ccgtaccgcg cggcgctgct cctcggccag
       61 ccggtcaagc accaggggcg ttgcgcaccg accgctcgcg cagcgagatc cggcaactgc
      121 ggcaggccga ccgatccgga ctggccgggc ctcctccgca acagcagtcg cgcgatccac
      181 gacagccacg acaggcggca ggacgacacc cggcagcgcg acgcccgcct gcgcgtcgcc
      241 ctccacgacc tccgcgccgc actattggcc ggctcggctt cccggcccgc cgtttgcacg
      301 cagggttgct ggtgatcggg ttcgtcatga tcgtgttcac gggtcggctg ctgcagttgc
      361 agggcgtcga ctccactgtc tatgccgcag atgccaatgc gcagtactcg cacaaggtga
      421 cgctgccagc gcagcgcggc gcaattgttg atcgcaacgg ggcgacgctc gccgataccg
      481 tgaacgcgag cgacgtcatc ggcgatccgc tggttgccac caactcgacg accaccagtc
      541 cctggacgat ggcggcgacg ctctcgccga tccttggcgt caatcgcttc acgctcgtaa
      601 gcgatctaac aggcaagagt cagttcgtgt atttggccca tgccgtcacg ccacagattg
      661 ccaagcaggt gctggctctc gacctcccag gtgtgacgac cgagaacacg aacaagcggg
      721 agtacccggc gggcaacgtt gcagcgaacg tcgtcggatt cgttgggtcc gacgggaaag
      781 gtctcggcgg ccttgagtat cagtaccaga acctgctcgc gggcaaggag gggcaactca
      841 ccgtgcaggt gggtcacgac ggccaggtca ttccggacgg cgtcggtggc gggcaggcgg
      901 cggtgcccgg tcaaggcatc gaactcacga ttgaccgcga cattcagtgg aaagcacagc
      961 aggcgattgc cgcgcaggta caggcgacgg gtgctgatgg cgggtcggtg atcgtcatgc
     1021 agccgcggac cggtcagatc ctcgcgatgg cgacgacgcc cacgttcgac ccggcggacc
     1081 caggcgcagc tccggctgaa gaccgtggaa actctgcgct gtctgatgtc ttcgagcccg
     1141 gcagcaccaa caaggtcatc accatgtcgg ccgccattga ttccggcgtg ctgacaccga
     1201 tgtcgccgat cgatgtcccc ccgacgctcg tccgtgcggg caaggtcttc catgacgctg
     1261 agtcgcacgg actcctgcac ttgacgcttg ccggtgtgct tgcggagtcc agcaatatcg
     1321 gcacgatcct cacgtcggag aaagtcggcg tcgaccggct gtacgcgtac ctgcaggcat
     1381 tcgggctcgg ccagccgtcg ggcctgaact tccctggcga gagtcccggc atcctcgatc
     1441 cgctgcgcaa ctggtccgcg acccagcaat acaccgttcc ctacggccaa gggctggcgg
     1501 tgaatgcgct gcaggtcgcc accgtctact cgacgattgc gaatggcggg gtgcgcgtcg
     1561 aacccagcct ggtgaaggga ttcttgcaac ctgacgggtc cgtcaagccg gccaacgcac
     1621 cggcgcagac tcgggtcgtg agcccatcga cggccacgca ggtcgagggc atgctcgaag
     1681 ccgttaccac cgaccagggc actgcgcccg aggctcggat ccccggttat cgggtcgccg
     1741 ggaagactgg aaccgcgcaa cgagtagacc ccacgtgtgg gtgctaccgc ggctacacgg
     1801 cgtccttcgt cggcttcgca ccggcggaca atccgcagct cgtcgtcctt gtggtgctgg
     1861 acaatcccat ccacggttat tacggggggg ccgtcgcggc gccggtgttc aaggacgtca
     1921 tgtcgttcgc gctcgagtcg gagaagattc cgccgacggg gacgacgcca ccggtcgtca
     1981 aactcagcct tccctgacgg gtagggtgga gcgccgtgcc tggccaagac ccacgttcga
     2041 cctccttgcg tccggcggcg acgccggcgc ggcagtcggt cgaccttgct gatcgcctgg
     2101 gcacgac
//
LOCUS       DATCBJ010000111         4621 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_430478, whole genome shotgun sequence.
ACCESSION   DATCBJ010000111 DATCBJ010000000
VERSION     DATCBJ010000111.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 4621)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4621)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4621
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_430478"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(197..2182)
                     /locus_tag="VII50_02280"
     CDS             complement(197..2182)
                     /locus_tag="VII50_02280"
                     /inference="COORDINATES: protein motif:HMM:NF016047.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding repeat-containing protein"
                     /protein_id="HEY5049701.1"
                     /translation="MKLVRPGIVLASVALAIAVPLGAGAAATSSGIHPTTAELTGSVV
                     DTASHPLAGICATVVDSNRDVVNWSQTTAAGTYDITGIAPATYTITYEDCNRSTGPNV
                     QPQYYDNTQSYGSMQQQTLVAGQTKALDTQTMQAGAQVSLHLTDSHGNPVTGVEVKME
                     TTSAPGLLPSWDTYPQTDSGGNVTFVGLFPLIPYTVEYKSCPSTDCATVGYYPSDPNR
                     PGNASPVVLPVVGTPKSLTDILDLPPVESSTVTLTAAPASTAVAQLVTLTAAVTNPDG
                     SIPVGPVEFTVGTTEVGFADVDASGQAVVTTTLPLGTSDVLASFYPLEIGVTNSSGEL
                     WVTVPVVLPGGDGSGAGGGSSAGRGAVAPTGSVIGAPVARLAGTDRIATAVAVSQDSF
                     PAGNAGAVVLARADDYPDALVGGPLAAEKNAPLLLTEGSTLPGATATEVKRVLPVGGK
                     VYVLGGTSAIPTSVTSQLTSTGYTVVRYSGADRYATAVAVATALGSPTTVLLATGTNF
                     PDALAAGPAAAHVHGAILLTDGSTVPSETATYLDGARVTYAIGGPAAAAAPSATAILG
                     ADRYATAAAVATKFFPASTVVGVATGAGFPDALAGGAQLALRGAPLLLSSLGSVPTST
                     SSYLGADHSALTNVYVYGGIAVLNSGVVSQLAAAFVG"
     gene            complement(2318..3670)
                     /locus_tag="VII50_02285"
     CDS             complement(2318..3670)
                     /locus_tag="VII50_02285"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase class II"
                     /protein_id="HEY5049702.1"
                     /translation="MQGIDSWRALNVAQVPVWPDQQALRGVVEQLEQLPPLVVPEECD
                     RLRSRLADVAKGKAFLLQGGDCAETFADATVAKVRDKIKILLQMAVVLTYGASVPIVK
                     VGRMAGQYAKPRSKNTEFIDGVELDSYRGDAVNSIERTPAARVPDPARLLEAYHRAAA
                     TLNLIRAHATGGFADLRKVHSWNSDFVGRSPAGQRYESIAMDIDRALSFMQACGFDLD
                     RADVAHQVELFSSHEALLLDYEAALTRWDVDRSAYYDQSAHLVWVGERTRALDGAHVE
                     FARQIANPIGVKLGPSTTPDAAVALVDKLDPDRRPGRLMFVTRMGADRVLDVLPPIVE
                     KVTASGAEVVWSCDPMHGNTIESASGYKTRHFDDIVEEVTGFFEVHHGLGTVPGGLHV
                     ELTGEDVTECLGGGDDIVDADLSGRYETALDPRLNVGQSLELAFLVASMLQDGRRDRS
                     "
     gene            complement(3701..>4621)
                     /locus_tag="VII50_02290"
     CDS             complement(3701..>4621)
                     /locus_tag="VII50_02290"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004558616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent 6-phosphofructokinase"
                     /protein_id="HEY5049703.1"
                     /translation="GFRDGWRGPINADTVPLGIPQVRGILPRGGTILGSSRTNPAKID
                     GGFETIAANFETLGIDALVAIGGEDTLGVATQLDARGLNVVGVPKTIDNDLSATDYTF
                     GFDTAVNIATEAIDRLHTTAESHHRALIVEVMGRHAGWIALHAGMAGGANVILIPEIP
                     FDIEQVCAYIEHRFQSHYAPIVVVAEGATPAEGTMEVATGDLDAFGHVRLGGIGQRLA
                     DEIEKRTGKEARTTVLGHIQRGGTPTAFDRVLATRFGLHAIDAVHDGNFGMMVALRGT
                     DIELSKLADATGELKTVPAERYAEAQVLFG"
BASE COUNT          822 a         1490 c         1524 g          785 t
ORIGIN      
        1 ctcgccagta gccaaacaag accaccacgc cggcaactgc tgccccaacg aatggtgcat
       61 agagcgaggc catccccgca accatcgcga tcccggcgac tacgagtcca attcctagcg
      121 cgggcgcgac ccatggacgg actggaccga aggcgtcgcg cttctcaggc atgccaccac
      181 gctacccggc gcccggtcaa ccgacgaacg ccgccgcgag ctgactaacc acaccactgt
      241 tgagcactgc gatgccgccg taaacgtaga cgttcgtcag cgcgctgtga tcggcgccga
      301 ggtagctgct ggtactcgtc ggcacgctac ccaggctcga cagcagtagg ggtgcgcctc
      361 tcaacgccag ctgcgcgcca ccggccaagg cgtcggggaa gcccgctccg gttgcgacac
      421 ccacaacggt tgaagcaggg aagaacttcg tcgcgacggc tgcggccgtc gcgtagcggt
      481 cggcgcccag gattgcggtg gccgagggcg ccgcggctgc ggcagggccg ccgattgcat
      541 acgtcacgcg agcaccgtca aggtaggtcg cagtctccga tggcaccgtc gagccgtcgg
      601 tgagcaggat cgcaccgtgc acatgcgctg cggctgggcc ggctgcgagc gcgtccggga
      661 agttggtgcc ggtcgcgagc aacactgtcg tcggactgcc gagggccgta gcaaccgcga
      721 ccgccgtcgc atatcggtct gcaccggagt aacgcacgac cgtgtagccg gtgctagtga
      781 gttggctagt aacactggtc gggatggctg atgtgccgcc gagaacgtag accttgccac
      841 ccactggtag aacacgcttg acctcggttg ccgttgctcc aggcagcgtc gagccttcgg
      901 taagcagcaa aggcgcgttc ttttcggccg caagcggtcc accgaccagt gcgtcggggt
      961 aatcgtcggc gcgcgccaag acgaccgctc ctgcgttacc agccgggaac gagtcctggg
     1021 aaacagcgac agctgttgcg attcgatcag tcccggcgag cctggcgacc ggcgcgccga
     1081 tcacagatcc ggttggtgcg acagctccac gacccgcact cgaaccacca ccggcaccgg
     1141 aaccgtcacc gcccggcaag acgaccggga cagtgaccca cagctcgcca gaactgttgg
     1201 tcacgccgat ttcgagggga tagaagcttg caagaacatc cgaggtgccg agcggaagag
     1261 tcgttgtcac gaccgcctga ccggacgcgt caacgtccgc gaagccgacc tctgtcgttc
     1321 cgacggtgaa ttccacgggt ccgacgggga tggacccgtc agggttggtc actgcggcgg
     1381 tcagtgtgac gagctgggct acggcagtag acgctggcgc ggccgtcagc gtgacagtgc
     1441 tgctctcgac agggggtagg tcgagaatgt cagtaaggct cttcggtgtg cccacgaccg
     1501 gcagcactac tggcgacgcg ttccctgggc gatttgggtc gctcgggtag tagccaacgg
     1561 tcgcgcagtc agtagacggg catgacttgt attccactgt gtaggggatc agcgggaaca
     1621 ggccgacgaa ggtgacgttg ccaccggaat ctgtttgcgg atacgtgtcc catgacggca
     1681 agagcccggg ggcggatgtc gtctccatct ttacctcgac gccagtcacc gggttgccgt
     1741 gggagtcggt caagtgcagc gagacctgcg cgccagcctg catggtctgc gtatcgagag
     1801 ccttcgtctg ccccgccacg agggtctgct gttgcataga cccgtaggac tgggtgttgt
     1861 cgtagtactg cggctggacg ttgggaccag tcgatcggtt gcagtcctcg taggtgatcg
     1921 tgtaggtcgc gggtgcaatc ccggtgatgt cgtatgttcc cgcagccgtc gtctggctcc
     1981 aattcacgac gtctctgttc gagtccacaa cggtcgcgca gatgccagca agcgggtggc
     2041 tggccgtatc cacgacactg ccggtgagct cagcagtggt tgggtgaata ccggagctgg
     2101 tcgcagctgc gccagcacct agaggtacag cgatggcgag cgcgacactc gcgagaacga
     2161 tccccggacg aacaagcttc atgaactttc ccccgtatcg gcgtacgtgg gcttattgtt
     2221 tcgccgccta cgcaaacctt ggggcgaagc taaaagtgca accgatcggc tgaggctcaa
     2281 aacgacccaa ttcaccgaag gcggtcaggt cagccaatca gctgcggtcg cgacggccgt
     2341 cttgaagcat cgacgcgacc aagaacgcga gctcgagcga ctggccaacg ttgagtcgag
     2401 ggtcgagcgc ggtctcgtag cggccagaca aatcggcgtc cacgatgtcg tcaccgccgc
     2461 ctaggcactc cgtgacgtcc tcgccggtca gctcgacgtg caagcctccc ggcaccgtgc
     2521 cgaggccgtg atgaacctca aaaaagccgg tgacctcctc gacgatgtcg tcgaagtgcc
     2581 gcgttttgta gccgctcgcg gactcaatcg tgttgccatg catggggtcg caactccaca
     2641 cgacctcggc gccggacgcc gttaccttct cgacgatggg cggcagcacg tcgagcaccc
     2701 gatccgcgcc catgcgggtg acgaacatca aacggccggg acgccggtcc gggtcgagct
     2761 tgtcgacaag cgcgacggcc gcgtcgggcg tcgtcgaagg cccgagcttg acaccgattg
     2821 gattcgcaat ctggcgggcg aattccacat gggcgccgtc gagtgcgcgg gttcgctccc
     2881 ccacccagac cagatgcgcc gactggtcgt agtacgccga ccgatcgacg tcccacctcg
     2941 tgagggcggc ttcatagtcg agaagaagcg cctcgtggct ggagaacaac tcgacctggt
     3001 gcgcgacgtc ggctcggtcg agatcgaaac cacacgcctg catgaacgac agcgcgcgat
     3061 cgatgtccat cgcgatcgac tcgtagcgct gcccagccgg actgcgaccg acgaagtcgg
     3121 agttccatga atggaccttg cgcagatcgg cgaaaccgcc ggtggcatgg gcgcggatca
     3181 ggttcaacgt tgcggccgcg cggtggtaag cctcgagaag cctggccgga tccgggaccc
     3241 gagccgccgg cgtccgctcg atcgaattga ctgcgtcgcc tcggtagctg tcgagctcga
     3301 ccccgtcgat gaattcggtg ttcttcgacc gcggcttggc gtactgcccc gccatccggc
     3361 cgactttcac gatcggcacg cttgcgccgt acgtcagcac gaccgccatc tgcagcagga
     3421 tcttgatctt gtcgcggacc ttggcgacgg tcgcatcggc gaaggtctcc gcgcagtcgc
     3481 cgccttgcaa caggaacgcc ttgcccttgg cgacgtccgc gaggcgcgac ctgaggcggt
     3541 cacattcttc cgggacgacg aggggtggca gctgctccaa ctgctcgacg acaccacgca
     3601 aggcctgctg gtcaggccat accggcacct gagcaacatt cagcgcacgc cacgaatcga
     3661 ttccctgcac ccgtggagtc taccggggtc gggagctgcc tcagccgaat aggacctggg
     3721 cctccgcgta gcgctcggcg ggcacggtct ttagctcacc ggtcgcgtcg gccagcttcg
     3781 agagctcgat gtcggtgcca cgcagagcga ccatcatgcc gaagttgcca tcgtggacgg
     3841 cgtcgatcgc atgcagcccg aatcgggtgg cgagcacccg gtcgaacgcg gtcggcgtac
     3901 cgccgcgctg gatgtgaccg aggaccgtcg tccgagcctc tttgccggtg cgcttctcga
     3961 tctcgtccgc cagccgctga ccgatgccgc ccaggcgcac gtgaccaaat gcgtcgaggt
     4021 cgccggtcgc gacctccatc gttccctcgg ccggcgtcgc gccctcggca acgacgacga
     4081 tcggtgcgta atggctctgg aaccggtgct cgatgtaggc gcagacctgc tcgatgtcga
     4141 acgggatctc cggaatcaag atgacgttcg cgccgccagc catgccggcg tgcagggcta
     4201 tccagcccgc gtggcggccc atgacttcga cgatcagtgc ccggtgatgc gattcggccg
     4261 tcgtgtggag gcggtcgatc gcctcggtcg cgatgttgac cgcggtgtcg aacccgaagg
     4321 tgtagtcggt ggcggacaga tcgttgtcga tggtcttcgg gacgccgacc acgttgaggc
     4381 cacgagcgtc cagctgcgtc gcgacgccga gcgtgtcctc gccgccgatc gcgacgagcg
     4441 cgtcgatgcc gagcgtctcg aagttggcgg cgatggtctc gaaaccgccg tcgatcttcg
     4501 ccgggttggt gcgcgacgat ccgaggatcg tgccgccccg gggcaggatg ccgcgaacct
     4561 ggggaatgcc gagtggaacg gtgtcggcgt tgatcgggcc acgccacccg tcacggaatc
     4621 c
//
LOCUS       DATCBJ010000112         2491 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_320397, whole genome shotgun sequence.
ACCESSION   DATCBJ010000112 DATCBJ010000000
VERSION     DATCBJ010000112.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2491)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2491)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2491
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_320397"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            199..2259
                     /locus_tag="VII50_02295"
     CDS             199..2259
                     /locus_tag="VII50_02295"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY5049704.1"
                     /translation="MTTNGKERTTTRRFSRGRITGLTLIAVLTLGLGYLHFAGSSTAV
                     SVPAGAHAGQLTLKSCTYNTEKGAYAADCGTLVVPENRADPHSRLIALPVIRIRAHSA
                     HPGDPIFRLQGGPGVTNMSFPDASRFTANHDVVLVGYRGVDGSSVLNCPEVTAALENS
                     ADFLGQKSLDAYSNAFRSCAKRLEANGVDLAGYTLPQRVDDLEAARKALGYHQIDLLS
                     ESAGTRTAMIYSWRYPTAIHSSVMIGVNPPGNFLWYPKTTDEQIARYARLCAQDATCH
                     KRTSDLAASLKRTVAHMPNRWLFFPIKKGNARIASFFGLMETTSNASPLDGPMTLDAW
                     LSAAHGDSSGLWFESLLAQITFPSVFVWGDLAAVAMADNQAGERYFSSSEPDRGSIIG
                     NPGTEFLFGGGGLLHAWPANPTDNEYTHVQTSKVPTLLIGGSLDFATPPEVATKELLP
                     YLPNGHQVILAGFGHTTSFWTEQTKASNHLINTFFDTGKVDASLYKPQAVNITPGSTQ
                     TAPAKAIAGAMMGFGLLTILSLLLMWRRAHKRGGFGRKASAMLRSVYLLVLGLGGWFI
                     GLIIVLVALPTVPLNSELMAVLSIGTPIGLGTYWAWVNRDRPARATRIGIWVALTGAL
                     VGALLGFNATSGLIALVTTIIGAAAGANLGLIALDIARIGERPETVPPIAIPAPIAIP
                     AVER"
BASE COUNT          437 a          915 c          730 g          409 t
ORIGIN      
        1 gttcaggccg tcgtcctcgc gtacgagagc gggctggtca cgcccggcgc gtagacgcgc
       61 ctctgtcctt cgacagaggc gcgagcggca aagatctgcg ctgaggagga gcgcagacgc
      121 gccctgcggg cgatgacagg cacagccggc ggtcccagag tgagccccag gcaacttgca
      181 ccgacctgag gagcagtgat gacgaccaac gggaaagaac gaaccacgac cagacgcttc
      241 agtcgcggtc ggatcaccgg cctgactctg atcgccgtcc tcacgctcgg gctcggctac
      301 ctccacttcg ccggcagttc tacggccgtc tccgtgcctg ccggcgcgca tgccggccag
      361 ctgacgctca agagctgtac ctacaacacc gaaaagggcg cctacgccgc cgactgcgga
      421 acgctcgtgg tgcccgagaa ccgcgccgac ccgcactcgc ggctgatcgc gctgccggtc
      481 atccggatcc gtgcgcattc ggcacacccc ggcgacccga tcttccgcct gcaaggcggc
      541 cccggcgtca cgaacatgtc gttcccagac gccagtcggt tcaccgccaa ccacgacgtc
      601 gtcctcgtcg gctaccgcgg cgtcgacggc tcttcggtgc tgaactgtcc cgaggtgacg
      661 gcggcgctcg agaactccgc agacttcctc ggccagaagt ccctcgatgc ctactcgaac
      721 gcgttccgct cgtgcgcgaa gcggctcgag gcaaacggcg tcgatctcgc cggctacaca
      781 ctcccccaac gggtcgacga cctcgaggcg gcgcggaaag ccctcggcta ccaccagatc
      841 gacctgttga gcgagagcgc gggcacccgc accgcgatga tctactcctg gcgctacccg
      901 accgccatcc acagttcggt gatgatcggc gtcaacccac ccggcaactt cctctggtac
      961 ccgaagacga cggacgagca gatcgcccgc tacgcccgcc tctgtgcgca agacgcgacg
     1021 tgccacaagc gcaccagcga tctcgccgca tcgctgaagc ggaccgtcgc gcacatgccg
     1081 aaccgctggt tgttctttcc gatcaagaag ggcaacgcac ggatcgccag cttcttcgga
     1141 ctcatggaga cgacgtcgaa tgcctcgccg ctcgacggac cgatgaccct ggacgcctgg
     1201 ctctcggccg cccacggtga ctcgagcggg ctctggttcg aatcgctcct cgcccagatc
     1261 actttcccgt cggtgtttgt ctggggcgac ctcgcagctg tcgcaatggc tgacaaccaa
     1321 gctggcgagc gttacttctc gtcaagcgaa cccgaccgcg gctcgattat cggcaacccc
     1381 ggaacggagt tcctcttcgg cggcggaggc ctcctgcacg catggccggc caacccgacc
     1441 gacaacgagt acacccatgt ccagacatcg aaggtgccga cgctcctgat cggcggcagt
     1501 ctcgacttcg ccaccccgcc cgaggttgcg accaaggagc tcctccccta cctgcccaac
     1561 ggccaccagg tcatcctcgc cggcttcggc cacacaacct ccttctggac cgagcagacc
     1621 aaggcgagca atcacctgat taacaccttc ttcgacacag gcaaggtcga cgcctcgctg
     1681 tacaaacctc aagcggttaa catcacgccc ggctccacac agaccgcacc cgccaaagcc
     1741 atcgccggcg cgatgatggg cttcggactc ctcacgatcc tgtcactgct actgatgtgg
     1801 cgccgggcgc acaagcgcgg cggcttcggg cgcaaggcga gcgcgatgct gcgctccgtc
     1861 tacctgctgg tgctcggcct cggcggatgg ttcatcggcc tgatcatcgt gctggtggct
     1921 ttgccgacag tgcccctcaa cagcgaactc atggctgttc tctccatcgg cacgccgatc
     1981 ggccttggca cctactgggc ctgggtcaac cgcgaccggc cggcccgggc cacgaggatc
     2041 ggcatctggg tcgcgctgac gggcgccctc gtcggcgcgc tgctggggtt caacgcaaca
     2101 tccgggctca tagcgctcgt caccacgatc atcggagcag ctgccggtgc gaacctcggc
     2161 ctgatcgccc tcgacatcgc acgaatcggc gagcggcccg agaccgttcc gcccatcgcc
     2221 attccagcgc ccatcgccat tccagcggtc gaacgctgac ccccggagtc cgttcgcgca
     2281 agcatgtggc ggtgtgctgg ttgccgccac atgctctgct gacgtacgtg cgcgctcggt
     2341 ggtctgaacg acgtcatcaa gttacggtgg atccagcgtg acccacgctg ccgacccgcg
     2401 tgctgacgcc tacatcgatg ccctgcccga ttggcagcaa gccatctgcc gcgagattcg
     2461 cgatctcgtc caccctgatc agcctcttca c
//
LOCUS       DATCBJ010000113         3204 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_356706, whole genome shotgun sequence.
ACCESSION   DATCBJ010000113 DATCBJ010000000
VERSION     DATCBJ010000113.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 3204)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3204)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3204
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_356706"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..767
                     /locus_tag="VII50_02300"
     CDS             <1..767
                     /locus_tag="VII50_02300"
                     /inference="COORDINATES: protein motif:HMM:NF041024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acVLRF1 family peptidyl-tRNA hydrolase"
                     /protein_id="HEY5049705.1"
                     /translation="LQGPRCSKDRAVPRTALFQGPRCSKDRAVPRTALWRGILGTVSH
                     VRTLDIGPERLERWVTGFGERHGGATWSAVGDAMQFTAADGATASLVAPFPPLPTAWA
                     SGQPVAALVDHALVPRVIGIVLVRLGGYAAGVFEGEKLIASKVGSRQVHGRSAAGGWS
                     QHRFARRREGQVKVALEKAADTAVEVILPLLGRLDAVVTGGDRQALHTVLADPRLSRL
                     RPLVTARILDVPDPRARVLRDSLQASRAIRVTLSEP"
     gene            complement(786..1178)
                     /locus_tag="VII50_02305"
     CDS             complement(786..1178)
                     /locus_tag="VII50_02305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEY5049706.1"
                     /translation="MHVQDGMNPDVLTIGPSHTLREASHLMAKRRVGAAVVSDAGGLG
                     ILTERDVLEAIGANQDPDVELVGDHLTKDLVFAAPDWNMDQAAAAMVRGGFRHLVVLE
                     GNDVVGILAMRDIVRSWLAVAASTEPAT"
     gene            complement(1261..2079)
                     /locus_tag="VII50_02310"
     CDS             complement(1261..2079)
                     /locus_tag="VII50_02310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HEY5049707.1"
                     /translation="MPDASPPNDLALAPNDDLSLALHLADIADEISTARFRATDLRVE
                     TKPDLTPVSDADRAVETAIRTKLADIRPRDAIVGEEFGANSASTGRRWVIDPIDATKN
                     FVRGVPVWATLIALMDGSEVIVGVASAPALGHRWWAGRGLGSWTSRAGADPRRNRVSA
                     VSSMADASLSYSDLFDWGSHASGFQQLAASVWRTRAYGDFWSHLLVAEGAVDISTEPD
                     VSIWDVAALVVIVEEAGGRVTGTDGAPSPHAPSIVCSNRLLHEAALATLASRHL"
     gene            2104..2652
                     /locus_tag="VII50_02315"
     CDS             2104..2652
                     /locus_tag="VII50_02315"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEY5049708.1"
                     /translation="MRWTVHGERTVYDSDWVRLGLVDVEIPGEGGGRRFEHHVLRMPH
                     PASGVVVHDAERGVLLLWRHRFVTDTWGWEIPAGRAEAGETPRQAAAREVVEETGWRP
                     GPLRELATYHPNNGMSDLTFNLFMADGATPVGPPTDPSESERIEWVPLPAVRDLVRGG
                     EVRDGLSLTALSMALLFELPEQ"
     gene            2665..>3204
                     /locus_tag="VII50_02320"
     CDS             2665..>3204
                     /locus_tag="VII50_02320"
                     /inference="COORDINATES: protein motif:HMM:NF021386.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2087 domain-containing protein"
                     /protein_id="HEY5049709.1"
                     /translation="MTTEDATPDAASVLALLVAGDRLPVVAALVLGAQTIGDIAQRTQ
                     LPARDVLRVLTRLESAGLASADGPHWTLHVEVLRDVVTGHRWAPVQQATSSEIEDESP
                     ANAAVLRAFFRDGRLVRVPAQRTKRLVVLDKLARDFEPGVRYPEVEVNQKLTAHHPDY
                     AALRRYLVEEDFLARENGVY"
BASE COUNT          523 a         1039 c         1088 g          554 t
ORIGIN      
        1 tgctccaggg accgcgctgt tccaaggacc gcgctgttcc aaggaccgcg ctgttccaag
       61 gaccgcgctg ttccaaggac cgcgctgttc caaggaccgc gctgtggcgc ggcatactcg
      121 gcaccgtgtc acacgtgcgc actctcgaca tcggacccga acgcctcgag cgctgggtaa
      181 ccggattcgg cgagcggcac ggtggagcga cgtggagcgc ggtcggcgac gccatgcaat
      241 tcaccgctgc ggacggggcg accgcttcac ttgtcgcccc gtttcccccg ttgccgacgg
      301 cgtgggcgag cggacaaccg gtcgctgcgc tcgtcgatca cgcactggtt ccgcgcgtaa
      361 tcgggatcgt gctcgttcgc ctcgggggat atgccgcagg ggtgttcgag ggtgagaaac
      421 tgatcgcgtc caaggtggga tcgcgacagg ttcacgggcg gtcggcagcg ggcggctggt
      481 cgcagcaccg cttcgcacgg cggcgtgagg gtcaggtcaa agtggccctg gagaaagcgg
      541 cggacacggc cgtcgaggtg atactgccgt tgctcggacg attggacgca gtcgtcaccg
      601 gcggcgaccg gcaggcgttg catacagtgc tcgccgaccc gcggctgtcg cgattgcggc
      661 ccttggtgac cgcacgcatt ctcgatgtgc cagaccctcg cgcgcgggtg ctgcgcgaca
      721 gccttcaggc cagccgtgcg atccgggtga ctttgtcgga gccctagtaa gcgcagcccg
      781 ccgttttacg tcgccggctc cgttgacgcg gcgacagcca gccaactgcg aacgatgtca
      841 cgcatggcga ggatgccgac gacgtcgttt ccctcgagga cgacgaggtg tcggaacccg
      901 cctcgaacca tcgccgctgc agcctgatcc atattccagt cgggagccgc gaacacgaga
      961 tccttggtca ggtggtcgcc gacgagttcg acgtcgggat cctggttcgc gccgatggct
     1021 tcgagtacgt cgcgctcggt gagaatgccg agcccgcccg catcggagac cacggcggcg
     1081 ccgacccgcc gcttcgccat caggtgactt gcttcgcgca gggtgtggct tggtccgatc
     1141 gtcaaaacgt cggggttcat accgtcctga acatgcatcg cagcctccag gggcgcacgg
     1201 cgcaccacgt gcacctcgct tcatcgtgga cccaccggag cgctcccaca aggaccggcc
     1261 tcacagatgt cgagaggcaa gggtcgcgag cgcggcctcg tgcagtagcc ggttgctgca
     1321 cacgatgctc ggcgcgtgtg ggctcggtgc accgtccgta ccagtgaccc gaccacctgc
     1381 ttcttcaacg atcacgacga gcgcggcgac gtcccagata gaaacgtctg gttcggtgga
     1441 gatatcgacg gcgccctcgg cgaccagcag gtgtgaccag aaatcgccgt aagcccgcgt
     1501 gcgccatacc gacgccgcga gttgttgaaa gccgctcgca tgcgaacccc agtcgaacag
     1561 gtcggagtag gacaacgacg catccgccat ggagctcacg gcggagaccc gattgcgccg
     1621 cggatctgcg cctgctcgcg aagtccagct gccgagacct cgcccggccc accatcgatg
     1681 gcccagggcg ggggcacttg ctactccgac gatgacctcc gatccgtcca tgagtgcgat
     1741 cagggtcgcc cacacgggca cacctcggac gaaattcttg gtggcgtcga tggggtcgat
     1801 cacccatcgc cgtcctgtcg aggccgagtt cgcgccgaac tcctcaccga cgatcgcgtc
     1861 gcggggtcga atgtctgcaa gcttcgtgcg aatggctgtc tcgaccgcgc ggtcggcatc
     1921 gctgaccggc gtgaggtccg gcttggtctc gacccgcagg tcggtagctc ggaagcgggc
     1981 cgtgctgatc tcgtcggcga tgtcagcgag gtgcaacgcg agcgaaaggt cgtcattggg
     2041 tgctagcgcc aggtcgttag gcggggatgc gtcgggcatg tcggcacgct atacggtcga
     2101 cgcatgcgct ggactgtcca tggtgagcgg acggtctacg acagcgactg ggtccgtctg
     2161 ggtttggtcg acgtcgagat tccaggtgag ggtggcgggc gacgattcga gcaccacgtg
     2221 cttcggatgc cgcaccctgc aagcggtgtc gtcgtccacg acgccgaacg cggcgtgctg
     2281 ctgctgtggc ggcatcgctt cgtcacggac acgtggggct gggagattcc cgcaggtcgt
     2341 gcagaagccg gcgagactcc ccggcaagcg gccgctcgcg aggtcgtgga agagaccggt
     2401 tggcgccctg ggccgttgcg cgagctcgcc acctatcacc cgaacaacgg catgagcgat
     2461 ctgaccttca acctcttcat ggccgacggc gccaccccgg tcggaccccc gaccgacccc
     2521 agcgagtcgg agcggatcga gtgggtgcca cttcccgcgg tgcgcgatct cgtgcgtggt
     2581 ggtgaggtac gcgacggtct cagcctgacc gcgctctcga tggcgctgct gttcgaactg
     2641 cctgagcagt aaggcaattc gatggtgact accgaagatg cgacacctga cgcggccagc
     2701 gtgttggcac tgctcgtggc aggcgatcgg cttccggtcg tcgcggccct tgtgctcgga
     2761 gctcaaacga tcggcgacat tgcgcagcgc acgcaattgc cggcacgcga tgtcttgcgg
     2821 gtgctgacgc ggctggagtc cgccgggctt gcgtccgccg atggtccgca ctggacgctg
     2881 cacgtcgagg ttcttcggga cgtggtgacc gggcaccgtt gggcgcctgt gcagcaggcc
     2941 acgtccagcg aaatcgagga tgaatcaccg gcgaacgccg ccgttctccg tgcatttttc
     3001 cgagacggcc gcctcgtccg tgttcccgct caacgcacga agcggctggt ggtgctcgac
     3061 aagctcgcgc gtgacttcga accgggcgtg cggtatcccg aagtcgaggt caatcaaaag
     3121 ctgaccgctc accaccccga ctacgccgcc ctgcgccgat acctagtcga ggaggatttt
     3181 ctcgcgcgtg agaacggcgt gtac
//
LOCUS       DATCBJ010000114         2799 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_24241, whole genome shotgun sequence.
ACCESSION   DATCBJ010000114 DATCBJ010000000
VERSION     DATCBJ010000114.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2799)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2799)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2799
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_24241"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..159)
                     /locus_tag="VII50_02325"
     CDS             complement(<1..159)
                     /locus_tag="VII50_02325"
                     /inference="COORDINATES: protein motif:HMM:NF024949.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEY5049710.1"
                     /translation="MHLVCTIRYIDGMSDMPSAGALLRTARERAGLSQAELAARAGVT
                     QSVISVYES"
     gene            375..587
                     /locus_tag="VII50_02330"
     CDS             375..587
                     /locus_tag="VII50_02330"
                     /inference="COORDINATES: protein motif:HMM:NF021474.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapB family
                     antitoxin"
                     /protein_id="HEY5049711.1"
                     /translation="MSKTLIDVDDSLLERARVILGPGATKKDAVNTALSKLVRLHDQR
                     DTIEWITATDPIADLRDPQVRAAARR"
     gene            584..1009
                     /locus_tag="VII50_02335"
     CDS             584..1009
                     /locus_tag="VII50_02335"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HEY5049712.1"
                     /translation="MSRYLVDNSVLQRLPRSSAVQTAVRALLDAEHELCCCALSLDEF
                     AYSARSAAEHTEASRRPRTSFLYLPLSPAIDQLILDIRAALWNAGTGRAAGVIDVALA
                     ATAVDSAATMLHYDSDFDHITAAYPPLHASWVVPRGSID"
     gene            complement(1190..2776)
                     /locus_tag="VII50_02340"
     CDS             complement(1190..2776)
                     /locus_tag="VII50_02340"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HEY5049713.1"
                     /translation="MNERVGTHVDLDALLERTRLASTPEACQDCLPEIARALELVTAS
                     ADRARLLICRARVRSFQSLDREACEDAVAAMALFEMVGDTELALDAASLAAAHASLLG
                     NVSLASDLATKSILGLESVNDDRLRMEISNRLGIFCYYYLDYDRAVEQFELSLAAAER
                     IGDGDRVCRELYNIADSLLLAAQQQRVSHLEIDTGFLERAEAVARRLLLEAAANPRLG
                     NYRLLAQVLCELDRVDEALQVLDRFWDEANADTPANHYADLTWVEARCLRLAGRVEEA
                     LAAARRDVRIIETSDDEHDLMLALEELAACEEAAGDLQSALAHTREVAVHMWAIHQRQ
                     TRQLVQQVWARVDMERDRTSLQAEASEAARSADEDALTGIGNRRVLERFLRDEAATET
                     EIACVIVDIDSFKAINDTFGHDIGDAVLCQIGRLLSTNTRPGQVAVRYGGDEFVVAMP
                     GVAPAGAHGFAERLRLAVCNIDWTAVAPDLHVTVSLGVACGPAKGWPVTFTAADGRLL
                     AAKRQGRNAVVTSSTSAVTA"
BASE COUNT          490 a          973 c          851 g          485 t
ORIGIN      
        1 cgactcatac acactgatga cgctctgagt gacacctgcg cgcgccgcca actcggcttg
       61 ggacaacccc gcccgttcgc gtgccgtgcg cagcagagcg ccggctgagg gcatgtcaga
      121 catgccatca atatagcgga tcgtgcatac caaatgcatc cgactttcaa gcagttgagg
      181 attttcggcc gtcccgcgat cagcgcgctg cgccctcgcc agtccatggc tccaggcgcc
      241 atagcctagc cgtgcaaact gctgacctgg tgcagcagcg acctccgcaa ggacgacgcg
      301 cctcgtattt ggggtcgact ccgtatatct actgtggata tactccctgc gatcggtgaa
      361 actgaggagg ccctatgtcg aagacactca tcgatgtcga cgactcgctg ctggagcggg
      421 ctcgagtgat ccttggccca ggcgcaacca agaaggacgc ggtgaacact gccttgtcca
      481 agctggtgcg cctgcatgac cagcgagaca ccatcgagtg gatcactgcc actgatccca
      541 tcgccgacct tcgtgacccg caggtcaggg ccgccgcgcg ccggtgagcc gctacctcgt
      601 cgacaacagc gtgctgcaac gactgcctcg cagctcggcc gtacagacgg cagtccgcgc
      661 gctgctggac gcggaacacg agctgtgttg ctgcgccctc agcctggacg agttcgcata
      721 tagcgctcgc tccgcagccg aacataccga agctagtcgc cggccgcgga cctcgttcct
      781 ctacctgccc ctgtcccccg ccatcgatca actcatcctc gacatccgcg ccgcactgtg
      841 gaacgccggc actggtcgtg ccgccggggt gatcgacgtc gcactcgccg ccactgccgt
      901 cgattcagca gccacgatgc tgcactacga cagcgacttc gaccacatca ccgcggccta
      961 cccaccactg cacgcctcat gggttgttcc gcgcggatca atcgactgac cgcccggccg
     1021 ggtccgcggc ttcgacctag tcccgcatgg cgtcggttgc cggagttgac cccacgacca
     1081 actccgcacg cagagcccat gtacgtaagt ttttgcgacc cctcggacac gatgtgatgt
     1141 ctcgagacat taggtgcacc tgtccgcgac acatagttca caccctcctt cacgctgtga
     1201 cggcgctggt agaggaggtg acgactgcgt tcctgccctg ccgcttggcg gcgagaagcc
     1261 gcccgtccgc ggcggtgaac gtgacgggcc agcctttcgc cggaccgcaa gcaaccccga
     1321 ggctcacagt tacgtggagg tcgggcgcga cggcggtcca gtcaatattg cagacagcga
     1381 gccgtaaccg ctcggcgaat ccatgggcgc ccgctggggc gacgccgggc atcgccacca
     1441 cgaactcatc cccgccgtag cgaacggcga cctgtccggg tcgcgtgttg gtcgagagga
     1501 gccgcccgat ttggcagagt acggcgtcgc cgatgtcgtg gccgaacgta tcgttgatcg
     1561 ccttgaacga atcgatgtcc acgatgacgc acgcaatctc ggtttcggtg gccgcctcgt
     1621 cgcggagaaa gcgttcaagc acacgcctgt tgccgatgcc ggtcagggcg tcctcgtccg
     1681 ccgatcgtgc cgcttccgac gcctcagcct ggagactcgt gcggtcccgc tccatatcca
     1741 cgcgcgccca gacctgttgc acgagctgtc ttgtctggcg ttggtgtatc gcccacatgt
     1801 gcacagcgac ttctcgggtg tgcgccagcg cgctctgcag gtcgccggca gcctcctcgc
     1861 aagcggcgag ctcctccagc gcaagcatca agtcgtgctc gtcgtcgctc gtctcgatga
     1921 tccgcacgtc gcgccgggca gcagcgagtg cttcctccac ccggccggcc aggcgcagac
     1981 atcgggcttc gacccaggtc aggtcggcgt agtgatttgc aggcgtgtcg gcgttcgcct
     2041 cgtcccagaa ccggtccagc acctgcaacg cctcgtccac gcggtctagc tcgcacagaa
     2101 cctgggcgag cagccggtag ttgccgagcc gcgggttcgc tgccgcctcg aggagcaatc
     2161 ttcgggccac ggcctcggcg cgttcgagga agccggtgtc gatttccagg tgggacactc
     2221 gctgctgctg ggcggcaagc agcagcgagt cagcgatgtt gtacaactcg cggcagaccc
     2281 tatcgccatc accgatgcgc tcggcggcgg cgagcgacaa ctcaaactgc tcaactgccc
     2341 ggtcgtagtc caggtagtag tagcagaaga tcccgagccg gttgctgatc tccatccgca
     2401 gacggtcatc gttgaccgat tccaggccca ggatgctctt tgtggccagg tccgacgcga
     2461 gcgagacgtt gccaagcagg gaggcgtgag ccgcggcgag gctcgccgcg tcgagcgcaa
     2521 gctcagtgtc gccgaccatc tcgaagagag ccatcgccgc gacggcgtcc tcacaggcct
     2581 cgcggtcgag cgactggaat gaccgcactc gcgcccggca gatgagcagc cgcgcccgat
     2641 cggcagaagc cgtcaccaac tcaagcgctc gcgcgatctc gggcaggcaa tcctggcaag
     2701 cctccggggt cgacgccaga cgcgtccgct cgagcaacgc gtcgagatcg acgtgcgtac
     2761 cgaccctttc gttcacccct gcctatcggc ccgcgcata
//
LOCUS       DATCBJ010000115         3397 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_430208, whole genome shotgun sequence.
ACCESSION   DATCBJ010000115 DATCBJ010000000
VERSION     DATCBJ010000115.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 3397)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3397)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3397
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_430208"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..714
                     /locus_tag="VII50_02345"
     CDS             <1..714
                     /locus_tag="VII50_02345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SEC-C metal-binding domain-containing protein"
                     /protein_id="HEY5049714.1"
                     /translation="GLSAEFLDEELIADAQAAYDTREQGLGTLPEGEPIMRELERKVV
                     LSVLDRKWREHLYEMDYLQEGIQLRSYGQRDPLVEYQREGFTMFTTMMDAIKEESVQL
                     LFNLEVQVAAPEQPAAAVADEAATQDAELPAKSAPARKTVAQEAAAAADGAGHGLPHL
                     VAPGLQEARRPTRLQYSAPTIDGDAGNLAPPVSGGPAAPTAGAEAHLMYEGTPRNAKC
                     PCGSGRTYKRCHGDPRSAV"
     gene            911..1318
                     /locus_tag="VII50_02350"
     CDS             911..1318
                     /locus_tag="VII50_02350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5049715.1"
                     /translation="MSKDGSLRCGARRVRLAGRRRVVLATEAARLGCPSLLADADCYG
                     GVIAQVLGLLDESPGLAAAVRQANAGNLDLRTLATLARVVGPNLRVLTGVARAEHWPE
                     LRPSGLVAAYAVARQLAALTVIDWDSVFSRTRS"
     gene            1333..1533
                     /locus_tag="VII50_02355"
     CDS             1333..1533
                     /locus_tag="VII50_02355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5049716.1"
                     /translation="MPRRNGSTLATLTDADHVVAVGSADPVGLQRLVRGLAELSEAIP
                     DCRPQVVVNPGAQGSDQRRCRG"
     gene            1844..2350
                     /locus_tag="VII50_02360"
     CDS             1844..2350
                     /locus_tag="VII50_02360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5049717.1"
                     /translation="MRIYLPATLPGLAAGGLGSEPVASQVEVAPAGARACAVTPALRE
                     WYRDGDIDELEYAAMTRAALASLRLLAGRGGDRRVVLAADVADNAVVFDPGDDPAAVR
                     VIDAVRLDQIVSAHVDDPSASYDVGAAVIMLERGDADDDDVQFAIDGLDDHELQWYAV
                     QEIAFLLR"
     gene            2447..>3397
                     /locus_tag="VII50_02365"
     CDS             2447..>3397
                     /locus_tag="VII50_02365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009075672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEY5049718.1"
                     /translation="MDAVTAVPSPTNEPPLHFAPGSTERARLELRLKEMSGEQLELTM
                     ALGSRRPMAGGEPIDVVAPHRRFTVLGRMREATAGDVADAVETAMAAAPAWRRLSFDD
                     RAAIFLKAADLLAGPWRDTINAATMLGQSKTCYQADIDAACELADFWRFNVHFAQGIL
                     AEQPISPPGVWNRLDHRPLEGFVLAITPFNFTSIAGNLPTAPAIMGNVVVWKPAPTSQ
                     LSSHYLMALLAEAGLPPGVITMVTGFGSAVSQVALPHRDLAGIHFTGSTPVFQQLWRA
                     VGDNLGSYRSYPRMVGETGGKDFVIAHPSADVDVLRTALVR"
BASE COUNT          535 a         1135 c         1169 g          558 t
ORIGIN      
        1 gggttgagcg cggaattcct cgacgaggag ttgatcgccg acgcgcaggc tgcctacgac
       61 acccgcgagc agggtctcgg cacgctgccg gaaggcgagc cgatcatgcg cgagctcgag
      121 cgcaaggtcg tgctgtcggt gctcgaccgc aagtggcgcg agcacctcta cgagatggac
      181 tacctgcaag aaggcatcca gctgcgctcg tacggccagc gcgatccgct ggtcgaatat
      241 cagcgcgaag gcttcacgat gttcacgacc atgatggacg ccatcaagga ggagtcggtc
      301 cagctgctgt tcaacctcga ggtacaggtc gcggcgccag agcagcccgc tgccgctgtc
      361 gccgacgagg cagcgacgca ggacgccgag ttgccagcga agtcggcacc cgcgcgaaag
      421 accgtggcgc aagaagccgc ggctgcggct gacggcgcag ggcatggcct gccgcatctg
      481 gtggctccgg gtttacagga ggcacgacgc cccacgcgcc tccagtactc cgcacccacc
      541 atcgacggcg acgcgggcaa ccttgcgcca ccggtgtcgg gtggcccggc ggcgccaacc
      601 gcaggtgcgg aggcgcacct catgtacgag ggcacgccgc gcaatgcgaa gtgcccctgc
      661 ggatcagggc gcacgtacaa gcggtgccac ggagatccgc gcagcgcggt ttagctcggc
      721 gtccggcagg tactgtccgc cgatgccggt cccgcactca tctccgcgga ggtgacgacg
      781 gcgatcgctg acggcgttgc cactggtggg gtcgatccgc tgcgcctcgg gtacgccgaa
      841 ccgcgcgccg cgctgcctaa agtgccggcg ctggccctcg tgccagcgga cgacgccgaa
      901 gccaacgatc gtgtcgaagg acggctcgtt gcggtgtggg gcccgacggg tgcgcctggc
      961 cggacgacgg cgggtcgtcc tcgcaacaga ggcggctcgt ctcggttgcc catctttact
     1021 agcggacgcc gattgctacg gcggtgtgat cgcgcaagtg ctcgggctgc tcgatgagtc
     1081 acccggcctt gccgccgcgg tacggcaggc gaacgccggc aaccttgatc tgcggaccct
     1141 cgcgaccttg gctcgtgtcg tcggtccgaa cctgcgggtg ttgacgggcg tcgcgcgcgc
     1201 cgagcattgg cccgagttgc ggccgagcgg gttggtcgcg gcgtacgcgg ttgcgcggca
     1261 acttgcggct ctcaccgtca tcgattggga ttcggtcttc agcaggacga ggagctgagt
     1321 tacgacacga cggtgccacg ccgcaacggc tcgacgctgg cgacccttac cgacgccgac
     1381 cacgtcgtcg ccgtcggatc cgccgatccg gtcgggctgc aacggttggt gcgcggcctc
     1441 gccgaattgt ccgaggcgat accggactgc cgtccacagg tcgtggtgaa cccgggtgcg
     1501 caagggtccg atcaacggcg atgccgagga tgaaatccgg aggcattgcc gcgctacgcg
     1561 gggatcgacg acgtcgtctt tgtgccctgc gacagcgtct cgctggatga ggccatcgcc
     1621 ggcggccaca ccctcggtga gatcgcgcca gattcgcccg tgcgccgcgc actgctgccg
     1681 ctcgctcggc gatacgcggg cgttcgcttg gttcgtccag gccgtcgacg tcgcgcccgg
     1741 ggcgacgggc ggcgctccgc ctgagtgtgc tcaacaggac cgtgcgcagc atgactgtgc
     1801 acagccggcg cgctgcccgc ccaaccgtgg gaccatgtcg cccgtgcgca tctacctacc
     1861 ggcgacgctg cccggcttag cggcgggcgg actcggctcc gagccggtcg cgtcgcaggt
     1921 cgaggtggcg ccagcggggg cacgcgcgtg cgcggtcact ccggcattgc gcgagtggta
     1981 tcgcgacggc gacatcgatg agctcgagta cgcggcaatg accagagcag ctctcgcctc
     2041 gctgcggttg ctggccggaa ggggcggcga ccggcgcgtc gtgctggcag ccgacgtcgc
     2101 cgacaacgcc gtcgtcttcg atccaggcga tgacccggcc gcagttcggg tcatcgacgc
     2161 ggtgcgcctc gaccagatcg tctccgccca cgtcgacgat ccgagcgcgt catacgacgt
     2221 aggcgccgcg gtcatcatgc tcgagcgcgg cgacgcggat gacgacgatg tgcagttcgc
     2281 gatcgacggt ctcgacgacc acgagctgca gtggtacgcc gtgcaagaga ttgccttcct
     2341 tctgcgctga ccgtgccagg atggatggag gccgtacccg gccctcagcc cagccaagcg
     2401 cagcccagcc cagcccataa ggcataccct cgcaggagtg gaagcaatgg atgccgtcac
     2461 cgcggttccg agtcccacca atgaaccgcc gctgcatttt gcaccgggaa gcaccgagcg
     2521 tgcccggctc gaactccgct tgaaggagat gtcgggcgaa cagcttgagt tgacgatggc
     2581 gctcggcagt cgccgtccga tggctggcgg tgaacccatc gatgtcgtcg caccacatcg
     2641 ccgattcacc gtgctggggc gcatgcgcga ggcgaccgcg ggtgacgtag ccgacgcggt
     2701 tgagaccgcg atggctgcag ctcccgcatg gcgtcggttg agtttcgacg accgcgcggc
     2761 catcttcttg aaggccgccg atctgctcgc cggcccgtgg cgcgacacga tcaacgccgc
     2821 cacgatgctg ggccagagca agacgtgcta ccaagccgac atcgacgcag cctgtgagct
     2881 cgccgacttc tggcgtttca acgtgcactt cgcgcagggc atcttggctg agcaaccgat
     2941 ctcgccgccg ggagtctgga accggctcga tcatcggccg ctcgaaggct tcgtgctggc
     3001 catcaccccg ttcaacttca cgtcgatcgc cggcaatctg ccgaccgcgc cggccatcat
     3061 gggcaacgtc gtcgtctgga aacccgcacc gacatcgcag ctctcgtcgc attacctgat
     3121 ggcgttgctt gccgaggccg gacttccgcc cggtgtgatc acgatggtga ccggtttcgg
     3181 ttctgcggtg tcgcaggtgg cgttgcccca tcgtgacctt gcggggatcc acttcaccgg
     3241 gtcgacgccg gttttccaac agctgtggcg ggccgtcggt gacaacctcg gcagctatcg
     3301 cagctacccg cggatggttg gcgaaacggg tggcaaggac tttgtcattg cgcatccgtc
     3361 ggcggacgtc gatgtgctgc gtactgcttt ggtgcga
//
LOCUS       DATCBJ010000116         3270 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_282059, whole genome shotgun sequence.
ACCESSION   DATCBJ010000116 DATCBJ010000000
VERSION     DATCBJ010000116.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 3270)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3270)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3270
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_282059"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(181..336)
                     /locus_tag="VII50_02370"
     CDS             complement(181..336)
                     /locus_tag="VII50_02370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5049719.1"
                     /translation="MLAAADDATDCPAPAWLRWLEQPAAVAASSAVTAAITVCDVVRI
                     TLYPPAL"
     gene            479..934
                     /locus_tag="VII50_02375"
     CDS             479..934
                     /locus_tag="VII50_02375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5049720.1"
                     /translation="MPAVPPLGTHATTVVAGLTCPDAGSPGQATSPSPKPLSPNVAIK
                     AVVGCETVQRSYPGLGTWNVQLAEVADSGLGPLLADLRASSDSMPSGILCPDFRILVP
                     WFELVDAAGTAINVAIPTDQCGAPKTATITALQALHFRVTDAVRLAPAA"
     gene            987..3086
                     /locus_tag="VII50_02380"
     CDS             987..3086
                     /locus_tag="VII50_02380"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HEY5049721.1"
                     /translation="MTASTQLTGQALLTGGQGSDYTARHLLVLEGLEAVRKRPGMYIG
                     STDSRGLMHCVWEIIDNSVDEALVGYCTKIDIVLRDDGSVEVRDDGRGIPVDIEPKTK
                     LSGVEVVMTKLHAGGKFGGGSYNATGGLHGVGASVVNALSVRLDIEVDRDGATHAMSF
                     RRGKPGIFIGDGGEAKFSERSGLTKTKRIAKKITGTRTRFYPDRQIFTKDAAVELAPL
                     LERARQTAFLVPSLTITVRDDRSAKGNEPSEQSFRYAGGLRDFVDFLAPDAGITDTLR
                     LTGSGSFDETVPMLDDAGHMTATDVQRDLDVDVALRWGVGYETIAKSFVNIIATPKGG
                     THVAGFERALVKVMNEQLRATKLLKVNDDPVIKEDILEGLTAVVTVRVAEPQFEGQTK
                     EVLGTPAASKIVADVVGRELKVFLTSTKRGDKQYARALLEKVGAASKTRLAARATKEA
                     QRRKNALESSSLPAKLADCRSEDVDRTELFIVEGDSALGTAKLARKSEFQALLPIRGK
                     ILNVQRAAVGDMLGNAECASIIQVIGAGSGRTFDLDAARYGKVIVMTDADVDGAHIRC
                     LLLTLFYRYMRPLLDAGRVFAAVPPLHRIEVVGVKEPVYTYSDAEMRRLLLDLEKKGK
                     KYKNPQRYKGLGEMDADQLAETTMEPGRRVLRRIRLEDAEVADNVFELLMGNDVAPRK
                     EFIVDSAALLDRARIDT"
BASE COUNT          567 a         1039 c         1102 g          562 t
ORIGIN      
        1 attcgggctg ttaggcgcga atcgggctcc cacatcgcgg tgcgcgggct tggagaattg
       61 tggtgcatca caccctaaga ttcgcgccgc cggagtcggc ggttcactat gaagatcggg
      121 cgtggggccg acgaggtaaa gcctggcggc ggtttgtgct tgaggtggtt cgtgaggtgg
      181 ttagagcgca ggggggtaca gggtgatgcg gacgacgtcg cagacagtga tggcggcagt
      241 caccgcgctg ctggcggcaa ctgcggcggg ctgttccagc catcgcagcc aggccggggc
      301 cgggcaatcc gtggcgtcgt ctgcagctgc gagcactcag ctcacgagtg cggccacctc
      361 ggccagcccg gccagctccg cctcggccgg gccggcatcg tctggtgagg cagcactgtc
      421 gacgcctgtc ccgcctgtcc cttccgtcgc ggcgcctgtc gcgtcacgaa gtggagccgt
      481 gccggctgtt ccgccgctcg ggacacacgc gaccacggtt gtggccgggc tgacctgccc
      541 ggatgccggg tctcctgggc aggcgacatc gccgtccccg aaaccgcttt cgccaaacgt
      601 cgcgatcaag gctgtggtgg gttgcgagac tgtccagcgc agctatccgg gtttgggcac
      661 ctggaacgtg cagcttgccg aggtcgccga ctcaggtctt ggaccactct tggccgacct
      721 gcgggcgtcc tccgattcca tgccgagcgg catcctgtgt cccgactttc ggatcttggt
      781 gccatggttc gagctcgtcg acgccgcagg gaccgcgatc aacgtcgcga tcccgacgga
      841 tcagtgcggc gcaccgaaga ccgcaacgat cacggcgctc caggccttgc actttcgggt
      901 gaccgacgcg gtgcggctgg cgcccgccgc ctgagtcagc ccggtagcct gcacaggcac
      961 ccgcgagaaa gcgcaggatc taccgcgtga cggcatcgac ccaactgacc ggccaggcgt
     1021 tgctcaccgg cgggcaaggc tccgactaca cggcacggca tctcctggtg ctcgagggtc
     1081 tcgaggccgt ccgcaagcgc cccgggatgt acatcggctc gaccgacagt cgcgggctga
     1141 tgcattgcgt gtgggagatc atcgacaact ccgtcgacga ggcactggtc ggctactgca
     1201 cgaagatcga catcgtgttg cgtgacgacg gctcggtcga ggtccgggac gacggccgcg
     1261 ggatcccggt cgatatcgag cccaagacca agctgtccgg cgtcgaagtc gtcatgacca
     1321 agttgcacgc tggcggcaag ttcggtggag gctcgtacaa cgcaacgggt gggctgcacg
     1381 gtgtgggtgc gagcgtcgtc aacgcgctgt cggtccggct cgacatcgag gtcgaccgcg
     1441 acggcgctac tcatgccatg agctttcgtc ggggtaagcc cggcattttc atcggcgacg
     1501 gcggcgaggc gaagttcagc gagcggtcag gcctgacaaa gaccaagcgg atcgcgaaga
     1561 agatcaccgg cacgagaacg cggttctatc ccgaccggca gatcttcacc aaggatgccg
     1621 cggtcgagct cgccccgctt ctcgaacgcg cccggcagac ggcctttctc gtgccgagcc
     1681 tcacgatcac ggtgcgcgac gaccgaagtg ccaagggcaa tgagcccagc gagcagtcgt
     1741 tccgctacgc cggcggtctg cgtgacttcg tcgatttcct cgcgcctgat gcgggcatca
     1801 ccgacacctt gcggctcacg ggttcgggat cgttcgacga aaccgtgccc atgctcgatg
     1861 acgccgggca catgaccgcc accgacgttc agcgcgacct cgacgtcgat gtggcacttc
     1921 gctggggcgt cggctacgag accatcgcga agtcattcgt caacatcatt gcgacgccta
     1981 agggcggcac gcatgttgcg ggtttcgaac gtgcgctggt caaggtgatg aacgagcagt
     2041 tgcgcgcgac caagctgctc aaggtcaacg acgacccggt catcaaggaa gacatcctcg
     2101 aagggctcac cgcggtggtt accgtgcggg tagccgagcc gcagttcgag ggccagacga
     2161 aggaagtcct gggcacgccg gctgcgtcga agatcgtcgc tgatgttgtc ggccgcgaac
     2221 tcaaggtgtt cctcaccagc acgaagcgtg gcgacaagca atacgcgcgc gcgttgctcg
     2281 aaaaggtcgg ggccgcgtcg aagacccggc tcgccgcccg cgccaccaaa gaggcgcagc
     2341 gtcgcaagaa cgcgctcgaa tcgtcgtccc tgccggcgaa gctggctgat tgcaggagcg
     2401 aggacgtcga ccggaccgag cttttcatcg tcgagggcga ctcggctctc ggcaccgcga
     2461 agctcgcccg caagagcgag ttccaggcac tgcttccgat ccgcggcaag atcctgaacg
     2521 tgcagcgggc agcggtcggc gacatgctgg gcaatgccga gtgtgcatcg atcatccagg
     2581 tcatcggcgc cggctcaggg cgcacgttcg atctcgatgc cgcgcgctac ggcaaggtga
     2641 ttgtgatgac cgatgcagac gtcgacggcg ctcatatccg ttgtctgttg ctcacgttgt
     2701 tctaccggta tatgcgtccg ctgctcgacg ccggtcgggt ctttgccgcg gtgcctccgc
     2761 tacatcgcat cgaggtcgtc ggcgtcaaag agccggtcta cacctacagc gacgccgaaa
     2821 tgcgtcgtct gctgctcgac cttgagaaga agggcaagaa gtacaagaac ccgcagcgat
     2881 acaagggcct cggggagatg gacgccgatc agctggcgga gacgaccatg gaacccggcc
     2941 gtcgggtctt gaggcggatc cggctcgagg acgccgaggt cgcggacaat gtcttcgagc
     3001 tgctgatggg aaacgacgtc gcaccacgca aggagttcat cgtggactcc gcggcgcttc
     3061 tcgaccgggc tcgcatcgac acctgaggcc cacatcatgt cgacgacgtt ggcaggcgat
     3121 cgtgccgcca aggcgccggc caccaccccc gtgtgcggcc ggcgtcctgc ggctcccacg
     3181 gcttagacgc atctcggcgc gtattggttc gctgcccttc gaggtttggt tcacctcggg
     3241 gcagcaattt gcgttcgatg ggctaagccg
//
LOCUS       DATCBJ010000117         2078 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_466570, whole genome shotgun sequence.
ACCESSION   DATCBJ010000117 DATCBJ010000000
VERSION     DATCBJ010000117.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2078)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2078)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2078
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_466570"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..438
                     /locus_tag="VII50_02385"
     CDS             <1..438
                     /locus_tag="VII50_02385"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEY5049722.1"
                     /translation="EQEAWWRGAHLDPDAIPLLDSLRARGLRVGLCSNAPYRIQSLHS
                     QLAYLGLDGHLDAVTFSAEVGWRKPSPRMFAAALSALGTIPERTVMVGDSETADIAGA
                     HAAGMRAVLIRRSGNQASGDADEVIAALHELPGALRNMGLYLG"
     gene            441..692
                     /locus_tag="VII50_02390"
     CDS             441..692
                     /locus_tag="VII50_02390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5049723.1"
                     /translation="MEFKEYIKERCAERGISLHRLALLCDLNQIYFYQSVNKNKDNPP
                     PWVLRRAAPHLAVPYMELMIRAGYVRDADIDEWRQHSGS"
     gene            717..1925
                     /locus_tag="VII50_02395"
     CDS             717..1925
                     /locus_tag="VII50_02395"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF002882.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HEY5049724.1"
                     /translation="MTHRAIIHLDLDAFYASVEQLRRPELRGLPVIVGGAPSEDGRAQ
                     LNRGVVSAASYEARAFGVHSAMPLRTALRLCPQAVLVPVDFRAYRDASKTVFGIARDY
                     TPVIEPLSLDEAFLEVSGSVRRFGSPEHIAAEIRDRILTASGLHASFGVATCKTVAKI
                     ASDLRKPRGFVVVAPGDEAAFLAPLPLRRLPGLGPASERALSGLGISTLGQLAALPLD
                     TIQRRVGRASGTSLWERSRGIDTAPVTVPGAPRSVSREETFARDVAQRDALQARISEL
                     ASDVGARLRTGGWTARTVTLKLRYSDFTTITRQETLASSTATDTTVRDAAAALLDAAW
                     SGDAVRLLGVGVTGLAEAPQLDLFDQPKSDRIDHTLDRLRERFGQSAIRRGTGEGLHD
                     LDWRGDDLRR"
BASE COUNT          335 a          712 c          684 g          347 t
ORIGIN      
        1 gagcaggagg cctggtggcg cggcgcacac ctcgacccgg atgccatccc ccttctcgat
       61 tccctgcgcg ctcgaggact tcgtgtcggg ctgtgctcca acgcgccgta tcgaatccaa
      121 tcgctgcact ctcagcttgc ctatctcggt ttggacggcc atctcgatgc ggtgaccttc
      181 tcagccgagg ttggatggcg taagccgtcg ccacgcatgt tcgcggccgc gctgtctgcg
      241 ctcggaacga tcccggaacg cacggtcatg gtcggcgaca gcgagaccgc agacatcgcc
      301 ggggcgcatg ccgccgggat gcgcgcagtg ctcattcgca ggagcggcaa ccaggcctcg
      361 ggagacgctg atgaagtgat tgccgcactt cacgaattgc ccggtgcgct gcggaacatg
      421 ggcctatact tgggctaagc gtggagttca aggagtacat caaagaacgg tgcgcggaga
      481 gggggatttc cttgcaccgt ctcgcccttt tgtgtgatct gaatcagatc tacttctatc
      541 agagcgtcaa caagaacaag gacaatccac cgccgtgggt gttgcgccgc gccgccccgc
      601 accttgcagt tccgtacatg gagctgatga ttcgcgcagg ctatgtgcgt gacgcggaca
      661 tcgatgagtg gaggcagcac tcgggaagct gacgcggccg gcgacgggaa gcggtcgtga
      721 cgcaccgcgc gatcatccat ctcgacctcg acgcgttcta tgcatcggtc gagcagttgc
      781 gccgtcccga gctgcgcggg cttcccgtga tcgtcggcgg cgcaccctcc gaggacggtc
      841 gtgcgcagtt gaatcgcgga gtggtcagtg cggcgtcata cgaagcacgc gcattcggag
      901 tccattcggc aatgccactg cgcaccgcac tccggctgtg cccgcaggcc gtgctggtgc
      961 cggtcgactt tcgcgcgtat cgcgacgcgt cgaaaacggt cttcggcatt gctcgcgact
     1021 acacgccggt catcgagccg ctctcgctcg acgaggcatt tctggaggtc tccggatcag
     1081 tccgtcgctt cgggtcgccc gagcacatcg cagccgagat ccgcgatcga atcctcaccg
     1141 cgagcgggct ccacgcctcc ttcggggtcg caacctgcaa gacggtggcc aagatcgcct
     1201 ccgacctgcg caagccgcgg ggattcgtgg tcgtcgcgcc cggggacgaa gccgcattcc
     1261 tggcgccgtt gccgctgcgc aggctgcccg gacttggacc cgcatccgaa cgcgcgttga
     1321 gcgggttggg aatctcgacc ctcggccagc tcgccgcgct cccactcgac accatccaac
     1381 gccgcgtcgg gcgcgcctcc gggacgtcgc tctgggaacg gtcgcgggga atcgacacgg
     1441 cgccggtcac ggtgccgggc gctccacgaa gcgtctcgcg ggaggagacg ttcgcccgcg
     1501 acgtcgccca gcgggacgcg ctccaggcac gcatctccga gctcgcgagc gatgtggggg
     1561 cgcgcctccg caccggcggg tggacggccc gcaccgtgac gctcaagctc cgctactcgg
     1621 acttcaccac catcacccgc caggagacgc tggcgtcgtc gaccgcgacc gacaccaccg
     1681 ttcgcgacgc ggctgccgca ctcctcgacg ccgcctggtc gggcgacgcc gtgcgcctgc
     1741 tcggggtggg cgtcaccggc ctcgccgagg ctccacagct ggatctgttc gaccaaccga
     1801 agagtgatcg catcgaccac acgctcgaca ggctccgcga acgcttcggt cagagcgcga
     1861 tacggcgcgg caccggcgag gggttgcacg acctcgactg gcgtggtgac gacctgcgcc
     1921 gatgagtcag agtcggtccg tgcccaaagc cacatccgcc ccggcttccc agccgcgtcc
     1981 acgcacctgg ctcgagatgc gtgagcgaat cgagaagatc ctcgaacgtc ggaccggcga
     2041 gggcatctcc gtgtgggtgg cgcgcgttgc cgagctcg
//
LOCUS       DATCBJ010000118         2376 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_172077, whole genome shotgun sequence.
ACCESSION   DATCBJ010000118 DATCBJ010000000
VERSION     DATCBJ010000118.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2376)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2376)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2376
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_172077"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            168..683
                     /locus_tag="VII50_02400"
     CDS             168..683
                     /locus_tag="VII50_02400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MogA/MoaB family molybdenum cofactor
                     biosynthesis protein"
                     /protein_id="HEY5049725.1"
                     /translation="MPTPRALVITASDRASGGMYADKGGPLIIEALQAIGFEADGPIV
                     VPDGAPVEVALRSGIEAAYDVIITTGGTGLGPRDHTPQMTAQVLDYEVPGIAEAIRAT
                     ARIDVPTAVLGRGLAGVAGGTLIVNLPGSVGAVRDGMAVLAPILQHAVEQLAGNNHDG
                     GPADHDPYHHG"
     gene            690..1319
                     /locus_tag="VII50_02405"
     CDS             690..1319
                     /locus_tag="VII50_02405"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HEY5049726.1"
                     /translation="MSVGWPVVLEHGPVRLRPLRLRDAGEWRMTRLMNREWLEPWEAT
                     TPGVTPSDGGRGYGDAVRALRRDARLGLCYPFALLVGEQFAGQVTVGAIARGSANSAY
                     LGYWIARHFAGRGIMPTALAMVIDHCFGDAGLHRVEANIRPENAASRRVVEKLGFRLE
                     GTRERYIHIAGAWCDHLSYAMTVEDVPGGLLPRWLAAGGGARLSSADST"
     gene            1391..2323
                     /locus_tag="VII50_02410"
     CDS             1391..2323
                     /locus_tag="VII50_02410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5049727.1"
                     /translation="MSSLIVLGIVGIWILVLVPMWLNRHDADSASRSMDTFSTAMRVL
                     SRRGPSRTRFGRADRSDRTADGQYLVMPRDDWGVTVDNQIEISTTRRRLRVPLHLPSR
                     RQSYATSGRAQLIARRRRIAVGFAVLVIGLALSAEFVHTSWWFELGAGLALAGYLIHL
                     RTEAIRTSEMRRRRLARAARAAAPYPTARTAGGERYYAARVRHDDADVTPAAAAEGVS
                     ASGTVSASGTADGSRWEPIAVPLPTYVSKPVVSRPATAAPRADVESGPTIDLTQPGKW
                     IDSQVDVRYLLLDDDVAEVDELDVILARRRAVNH"
BASE COUNT          375 a          805 c          797 g          399 t
ORIGIN      
        1 ggcggtcgat cccgcagccg tcatcaccga catccgggtc gaagagaagc ccggcggctc
       61 gagcggcacc tggactcgcc caacctgacc tggcgtccgg ccttgcgaag gcccgaccga
      121 ggttgccccg cagcagcgtc cagctggcga tggactagtt tctcgtcatg ccgaccccga
      181 gggcgctcgt catcaccgcg tccgaccgcg cgtcgggcgg gatgtacgcc gacaagggcg
      241 gtccgttgat catcgaggcg cttcaggcga tcgggttcga ggcggacggt ccgatcgtgg
      301 tccctgacgg cgctcccgtc gaggtcgcct tacggtcggg aatcgaagcc gcttacgacg
      361 tcatcatcac caccggcggg accggccttg gcccgcgcga tcacactccc cagatgaccg
      421 cgcaggttct cgactacgag gtgccgggaa ttgcggaggc cattcgcgcg acggcacgga
      481 tcgacgtccc gactgccgtt cttggtcgtg gccttgccgg tgtcgccgga ggcacgctga
      541 tcgtgaacct gcccggatcg gtcggcgcgg tgcgtgacgg gatggcggtt ttggcgccga
      601 tcctccaaca tgctgtcgag caactcgcgg gcaacaacca cgatggcggt cccgcggacc
      661 atgaccccta ccaccatgga taggcgctcg tgagcgtggg ctggccagtc gttcttgagc
      721 acggcccggt gagactgcgg ccgctgcgac tgcgcgacgc cggcgagtgg cggatgacgc
      781 gcctgatgaa tcgcgagtgg ctcgaaccgt gggaagccac gacgcccggg gtcacgccgt
      841 ccgatggtgg ccgcgggtac ggcgacgccg ttcgagcgct ccgacgcgac gcccgtcttg
      901 gcctctgcta cccgttcgcc ctgctcgtcg gggagcaatt cgccgggcag gtcacagtcg
      961 gcgcgatcgc tcgcggctct gcaaactccg cctacctcgg ctactggatc gctcgccact
     1021 tcgccggtcg aggcatcatg ccgacagctc tcgcgatggt catcgatcac tgcttcgggg
     1081 acgccgggtt gcatcgcgtc gaggcgaaca tcaggcccga gaacgcagcg agccgccgtg
     1141 tcgtcgagaa gctgggcttc cggctcgagg gcacccgcga gcgatacatc cacatcgccg
     1201 gcgcctggtg tgaccacttg tcgtacgcga tgacagtcga agatgtgcca ggcgggctgc
     1261 ttccgcgctg gctcgcggcc ggcggcggtg ccagattgag cagcgccgac tcgacctgaa
     1321 gtcgacatat tccccgacac accaccgatg tactcgtcac atccgtcact cgtgtctagc
     1381 gtgacgaagt gtgagcagcc tgatcgtcct tggcatcgtg gggatttgga tcctggtgct
     1441 cgtcccgatg tggctcaacc ggcacgacgc cgacagtgca agccgctcga tggacacctt
     1501 ctcgacagcg atgcgcgtgc tctcgcgccg cggcccgtcg cgcacccgtt ttggtcgcgc
     1561 ggaccgctct gatcgcaccg ctgacggtca atacctcgtg atgccacgcg acgattgggg
     1621 agtcaccgtg gacaaccaga ttgagatatc aacgacccgt cgacggcttc gggtgccgct
     1681 gcatctgccg tcgcgccgac agtcgtacgc gacgagcggc cgagcacaat tgatcgcccg
     1741 ccgacgtcga atcgcggtcg gattcgcggt gctcgtcatc ggactcgcgc tctcggcgga
     1801 gttcgtgcac acgtcgtggt ggttcgaact aggcgccggc ctggcgctcg cgggctacct
     1861 gatccacctg cgcaccgaag ccatccgcac gtcggagatg cgccgccgcc ggctcgctcg
     1921 cgcagcgaga gcagcggcgc catacccaac agcgcggacg gcggggggcg agcgttacta
     1981 cgccgctcgc gtccggcacg acgacgcgga cgtcacgccg gcagccgcgg ctgaaggggt
     2041 cagcgcctcg ggaacagtta gtgcctcggg aacagcagat ggatcgcgat gggaacccat
     2101 cgccgtcccg ttgccgacgt atgtttccaa gcctgtggtc tcgcgtccgg ctactgcggc
     2161 gccgcgtgca gacgttgagt cagggccgac gatcgacctc acgcagccgg gaaagtggat
     2221 cgattcgcag gtcgacgtcc ggtacctgct gcttgatgac gacgttgctg aggtcgacga
     2281 actcgacgtg atcctcgctc gtcggcgggc cgtcaaccac tagcgcgccg tcaccgactc
     2341 agctgtcacc gtcggagggg accgccggac gccggt
//
LOCUS       DATCBJ010000119         2049 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_319623, whole genome shotgun sequence.
ACCESSION   DATCBJ010000119 DATCBJ010000000
VERSION     DATCBJ010000119.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2049)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2049)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2049
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_319623"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..397)
                     /locus_tag="VII50_02415"
     CDS             complement(<1..397)
                     /locus_tag="VII50_02415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5049728.1"
                     /translation="MVRRVVAVGAALGAGIAGFGFALVAVATAASASTALDVRGSWSE
                     VATTQGANYPQTVTWTTENFSTGALTGTDVGGGATFTMTGTLSGSKITTHLSEVGANY
                     TSDSVGTIKNAGGTLTWSGTFTDSVGHPAG"
     gene            534..1277
                     /locus_tag="VII50_02420"
     CDS             534..1277
                     /locus_tag="VII50_02420"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HEY5049729.1"
                     /translation="MYDGILAETTTISGHGGDLIEAYFARPLGPGPFGSVVVIHHLPG
                     YDAATKEIARRFAVLGYATLMPNLYSREAPGALPDDAAALVRAQGGVPDDRLVGDVGG
                     AAAHLRALPNSNGKVGVIGYCSGGRQSFLAATSLPLDAAVDCYGAFIVGVVPEGMPLK
                     VGPIVDRSPQLSCPLLGLFGSEDKYPSPEQVLELQQALDAAGKTYEFHSYDDAGHAFF
                     APDRPSYRPAAATEGWQRIETFFARYLGA"
     gene            1279..1539
                     /locus_tag="VII50_02425"
     CDS             1279..1539
                     /locus_tag="VII50_02425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6295 family protein"
                     /protein_id="HEY5049730.1"
                     /translation="MCTYITKTLPASGSAKGRAEWFDVTDVSVYFDHPVHAKAGHTLN
                     IDLRNPVLGPSARVAAELTADSARALAHAILAILDEAPPGLT"
     gene            complement(1536..>2049)
                     /locus_tag="VII50_02430"
     CDS             complement(1536..>2049)
                     /locus_tag="VII50_02430"
                     /inference="COORDINATES: protein motif:HMM:NF025736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEY5049731.1"
                     /translation="PGLVRVVNLFYLVAHIGSLVIFLPWMFIRHRAQYRRWRNIIAVF
                     TGVSLLIQLVSVAPPRLLPQFGFIDTAALYHESAYTHLGRGLTGQLSSMPSIHVGWAI
                     AIAMAVISTSTSRWRWLVLAQPILTVYAVVATANHFWLDCVAAAGLVLLVFAAVNGWG
                     RRKLGAAELA"
BASE COUNT          348 a          670 c          687 g          344 t
ORIGIN      
        1 tgccggccgg gtgtccgacg ctgtcggtga acgtgccgct ccacgtgagg gtgccgccgg
       61 cgttcttgat ggtgcccacg gaatcggagg tgtagttggc gcccacttcg gacaggtgtg
      121 tggtgatctt cgagccgctg agggttcccg tcatggtgaa cgtcgcaccg ccgccgacgt
      181 cggttcctgt tagtgctccc gtcgagaagt tctcggttgt ccaggtcacg gtctgggggt
      241 agttcgcccc ctgggtggtc gccacctccg accacgaccc gcgaacgtcg agtgcggtac
      301 tcgccgatgc ggcggtcgcg accgctacca gcgcgaaccc gaaacccgcg atcccggcgc
      361 cgagtgctgc gccgaccgct actactcgcc gaaccattgc gtgcctctca cctggatccg
      421 ttaagtgatg atgacgccaa cgccgacgcc gtgtcagtgt tttgcgcagg tcgacataac
      481 gtggtcgcgc gccggtaggc gaacccagcc aacccaacag cggaggagcg tcgatgtacg
      541 acgggatcct ggccgaaacg accaccatca gcggccacgg tggcgacctg atcgaggcct
      601 acttcgctcg cccgcttggg cctggtccgt tcgggtccgt cgtggtgatc caccacctgc
      661 ccgggtacga cgctgcgacc aaggagatcg cccgtcgctt tgcggtgctc ggttacgcga
      721 ccctgatgcc gaatctgtat tcgcgggagg ctcccggtgc cctgcccgac gacgccgctg
      781 cgttggtgcg cgcccagggt ggcgtgcccg acgatcgctt ggtcggcgac gtcggtgggg
      841 ccgcggccca cttacgagcc ttgccgaatt cgaacgggaa ggtcggcgtc atcgggtatt
      901 gctcgggagg tcggcagtcg ttcctcgccg caaccagtct tccgctcgat gcggcggtcg
      961 actgttatgg cgcgttcatc gtgggcgtgg tgccggaggg catgccgctc aaggtcggac
     1021 cgatcgtgga tcgctcgccg cagctgagct gcccgctgct cggtttgttc ggatcggaag
     1081 acaagtaccc gtcgcccgaa caggttctcg agttgcagca ggctctggac gctgcaggaa
     1141 agacgtatga gttccacagc tacgacgacg ccggtcacgc gttcttcgcc cccgaccgac
     1201 cgagctaccg gccagccgct gctaccgaag gctggcagcg catcgagacg ttcttcgcgc
     1261 gctacctggg cgcatgagat gtgcacctac atcacgaaga cgctgcccgc gagcggcagc
     1321 gctaaggggc gcgcagagtg gttcgacgtc actgacgtca gcgtgtactt cgatcacccc
     1381 gtgcacgcga aggccggcca caccctcaac atcgatctgc gcaatccggt gctcggcccg
     1441 agcgccaggg tcgcggcaga gctgaccgcc gactccgcac gggcactcgc ccacgcgatc
     1501 ctcgccattc tcgacgaagc gccgcccggg ctgacctaag cgagctcggc ggcgcccagc
     1561 ttccggcgtc cccacccgtt gacagcggcg aacacgagca gcaccaatcc tgcagccgca
     1621 acgcagtcga gccagaagtg gttggcggtc gcgaccacgg cgtaaaccgt caggatcggt
     1681 tgtgcgagga ccagccaccg ccagcggctg gtacttgtcg agatgacggc catcgcgatc
     1741 gcgatcgccc agccgacgtg gatcgacggc atcgaggaca gctgcccggt cagtcctcgc
     1801 ccgaggtgcg tgtatgccga ctcgtggtag agcgctgcgg tatcgatgaa accgaactgc
     1861 gggagcagcc gggggggagc gacggagaca agctggatga gcaacgagac gccggtaaac
     1921 accgcgatga tgttgcgcca gcggcggtat tgcgcgcggt gccggatgaa catccacggt
     1981 aagaagatga caaggctgcc gatgtgcgcg accaggtaga agaggttcac gacgcgcacg
     2041 aggcccgga
//
LOCUS       DATCBJ010000120         4580 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_101972, whole genome shotgun sequence.
ACCESSION   DATCBJ010000120 DATCBJ010000000
VERSION     DATCBJ010000120.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 4580)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4580)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4580
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_101972"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            234..422
                     /gene="rpmB"
                     /locus_tag="VII50_02435"
     CDS             234..422
                     /gene="rpmB"
                     /locus_tag="VII50_02435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014138233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HEY5049732.1"
                     /translation="MSAVCDVCGKGPGFGMSVSYSHRRTRRRFNPNIQTVRAVVGRGT
                     RKRLNVCASCIKAGKVTR"
     gene            complement(412..846)
                     /locus_tag="VII50_02440"
     CDS             complement(412..846)
                     /locus_tag="VII50_02440"
                     /inference="COORDINATES: protein motif:HMM:NF019928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HEY5049733.1"
                     /translation="MSAVEGVRASSPIPRAFACFVAADPARVWTALTNARNTGAFMYG
                     LSARSTWEIDAPIEFQSVGDSALIGRVVCVQPHRRLSYLVQAGPDDPPVYLTWLIRPS
                     PGGVTIRLHVDEIEYPDEDEEAEDIWLPMLAALQRTLTDVSE"
     gene            complement(952..1428)
                     /locus_tag="VII50_02445"
     CDS             complement(952..1428)
                     /locus_tag="VII50_02445"
                     /inference="COORDINATES: protein motif:HMM:NF014082.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HEY5049734.1"
                     /translation="MIVEPIGRVESSRSQAIDDDWDAVIATITLDGEQFTPDAVAGLG
                     EFSHVEVVFVFDGVDPEDIEQGARRPRGNAEWPRVGIFAQRAKMRPNRIGVTVCALLG
                     IDGLTLTVRGLDAIDGTPVLDVKPYMAEFGPRGDVRQPVWSRELMVGYWTAPEGAS"
     gene            complement(1425..1907)
                     /locus_tag="VII50_02450"
     CDS             complement(1425..1907)
                     /locus_tag="VII50_02450"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY5049735.1"
                     /translation="MNELQFTVAALDAPSSLELIEAVQQEYVARYGGRDITELAVAEF
                     TPPQGIFVIGRDGGQPVACGGVRLVEAAVGELKRMYVVPTARRRGIARALLDHLETEA
                     RTLGATRLRLETGLHQPEALALYASAGYADAEPFGHYAGAPLARHLAKNLIESGVPQS
                     "
     gene            complement(1904..2692)
                     /gene="thiD"
                     /locus_tag="VII50_02455"
     CDS             complement(1904..2692)
                     /gene="thiD"
                     /locus_tag="VII50_02455"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009084022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HEY5049736.1"
                     /translation="MTLPRVLTIAGSDSGGGAGIQADLKTMLACGVHGMSVVTAVTAQ
                     NSLGVQGYWELPPEAVRAQLDSVLGDIGVDAVKTGMLASSGLVSLVSAVLAGLDVPVV
                     VDPVGVSKHGDSLLAPDAVGAIKADLLPVATVVTPNLYEVAQLTGVKVNDEAGLREAA
                     EAVHALGPKWVLVKGGHLDGDAVDLLFDGMTQYLYRAPRLDNRHTHGTGCTLASAIAS
                     FLALGDAVPDAVQLAKDYVTGAIAAGFALGRGIGPVDHGWRRRP"
     gene            complement(2709..3674)
                     /locus_tag="VII50_02460"
     CDS             complement(2709..3674)
                     /locus_tag="VII50_02460"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEY5049737.1"
                     /translation="MSGALGRGPGIMTGMREGERTSGPAHELALPSSTDLTLIAIAVI
                     AVSTSGPLIVASAAPALALAFWRNGLGAAAMMSVALTRHRPELRRLTRREWRLIVTAG
                     VLLAAHFATWIPSLRYTSVASSTALVASQPVWAALLARTQGAVIGRSAWAGIVIAVLA
                     VVLLTGLDVRVSGRALSGDLLALVGGMLAAGYVTAGAAVRRTVTTTSYTSLCYSTTAV
                     LLLVVCLIGRQSLHGYSGADWARIVALTAGAQLLGHSVFNRVLKTTSPTIVSLSILFE
                     VPGATLIAAVFLHQHVRLEQVPAAVLLLGGLALVIRSGTRSMPAE"
     gene            complement(3858..>4580)
                     /gene="thiL"
                     /locus_tag="VII50_02465"
     CDS             complement(3858..>4580)
                     /gene="thiL"
                     /locus_tag="VII50_02465"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020661824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HEY5049738.1"
                     /translation="LADVAAMGAVTTSLLVGLAAPADLAAFWAERFADGLRDECDLVG
                     AGVVGGDVVRSDVLVIAVTALGDLEGRTPVTRSGAQSGDVVALAGRLGWAAAGLATLS
                     RGFRSPRLVVDAHRRPQPPYQAGPEAAKAGATSMIDVSDGLVADLGHVAAASGVRIDV
                     DSTAFAIPDALSQVASALSVDPLTWILRGGEDHALVATFGPGAELPAGWQVIGEVLDG
                     TGENTVTVDGLPYSAGGFDHFR"
BASE COUNT          797 a         1641 c         1462 g          680 t
ORIGIN      
        1 ccaccggcga gccgccgcgg catcgaggac ctccagcacc tcggcgtcct cccgatctgt
       61 actctcgccg gctagccagc gccggtccac tgggacgagg ctagccagcc tctgggacac
      121 cggccggttg gcgtgaccgg cgccgcgtcg gatagtctcg tgcggttgcc cggttcatga
      181 tctgcggcag ccagtgctgc tgtccgcccc acctcgcgct tggagtgtct cccgtgtctg
      241 ctgtctgcga tgtctgcggc aagggtccgg gcttcggcat gtccgtgtcg tactcgcacc
      301 gccgcacccg ccgccgcttc aacccgaaca tccagaccgt gcgtgcagtg gtcggccgcg
      361 gcacccgcaa gcggctgaac gtgtgcgcct cctgcatcaa ggcgggcaag gtcactcgct
      421 gacgtcggtc agcgttcgct ggagagccgc cagcatgggc agccagatgt cctcggcctc
      481 ttcgtcctcg tccggatatt cgatctcgtc gacgtgcagc cggatcgtga cgccgcccgg
      541 gctcggccga atcagccagg tgaggtagac cggcggatcg tcagggccgg cctgcacgag
      601 gtacgacagc cgacgatgcg gctgtacgca cacgacgcga ccgatgagcg cgctatcccc
      661 cactgattga aactcgatgg gcgcatcgat ctcccaggtc gagcgcgccg acagcccgta
      721 catgaatgcg ccggtgttcc gggcattggt caatgccgtc cagacgcggg ccggatcggc
      781 cgcaacgaaa cacgcaaacg cccgcggtat cggcgacgaa gcccgcacac cctcaacggc
      841 gctcatgtca ccaactccct gcgccgggcg acgtgccggc ggctcggcga tttcgccgag
      901 ggtacggcag cgcaggacgc ctgtcgatga tcaaactcac cgattcgtac ctcagctagc
      961 gccttcgggc gcagtccagt agccgaccat cagctcgcgc gaccacaccg gctggcggac
     1021 gtcgcctcgc ggaccgaact ccgccatgta cggcttgacg tcgaggaccg gcgtgccgtc
     1081 gatggcgtcc aggccccgca ccgtcagtgt cagcccgtcg atgccgagca gcgcacagac
     1141 ggtgacaccg atccggttcg ggcgcatctt cgcgcgctgc gcgaaaattc ccacccgcgg
     1201 ccactccgcg ttgccgcgcg ggcggcgagc gccctgttcg atgtcttccg ggtcgacccc
     1261 gtcaaacacg aagacgacct ccacgtggga gaactcacca agaccggcca cggcatcggg
     1321 ggtgaactgc tcgccgtcca gcgtgatcgt tgcgataacg gcatcccagt cgtcatcgat
     1381 cgcctgactg cgcgacgact cgacccgccc gatgggctcg acaatcacga ctgcggtacc
     1441 cccgattcga tgaggttctt cgccagatgc ctcgcaagtg gcgcgcccgc gtagtgtccg
     1501 aacggttcag cgtccgcata accggcgctt gcgtacagtg ccagtgcctc aggctggtgc
     1561 agtccagttt cgagccgcaa ccgcgtcgct ccgagtgtgc gcgcctcggt ttcgaggtga
     1621 tccagcaagg cccgggcgat accgcggcga cgcgcggtag ggacgacata catgcgcttg
     1681 agctcgccga cggcagcttc gacgaggcgc acgccaccgc acgcgacggg ctgaccgccg
     1741 tcccgcccga tcacgaaaat gccttgtggc ggcgtgaatt cggccaccgc gagctcggtg
     1801 atgtcgcgac cgccgtaccg cgcgacgtat tcctgctgaa cggcctcgat gagctcgagc
     1861 gatgacggtg catcgagcgc cgccacagtg aattgaagtt cattcacggc cgccgccgcc
     1921 agccgtgatc gactggcccg atgccacggc cgagcgcgaa tccggcggcg attgcgccgg
     1981 tgacgtagtc cttcgcgagc tgcacggcgt cgggaaccgc atcgcctagc gcgagaaacg
     2041 acgcgatggc actcgccaac gtgcagccgg tgccgtgcgt gtgcctgttg tccagccggg
     2101 gcgcccggta gaggtactgc gtcatcccgt cgaagagcag gtcgacggcg tcaccgtcga
     2161 gatgcccacc cttgaccagc acccacttcg gcccgagcgc gtgcacggct tcggctgcct
     2221 cgcgcagccc cgcctcgtcg ttcaccttca cgccggtcag ttgcgcaacc tcgtacaggt
     2281 tcggagtcac gacagtggcg accggcagca gatcggcttt gattgcacca accgcgtcgg
     2341 gcgccagcag cgaatcaccg tgcttcgaga ccccgaccgg atcgaccacc acagggacgt
     2401 cgaggccggc cagaaccgca gagacaagcg aaaccaggcc ggacgacgcg agcatccctg
     2461 tcttgactgc gtcgacaccg atgtcgccta gcaccgaatc gagctgtgcc cggacggctt
     2521 ctggcggcaa ctcccagtag ccctgcacgc cgagggagtt ctgcgcggtg accgcggtga
     2581 ccacgctcat gccatgcacg ccgcacgcca gcatcgtctt caggtcagct tgaattcctg
     2641 ccccgccgcc cgaatcagac cccgcgatcg tgagcactcg cggcagcgtc atgcgtccat
     2701 tctcccagct actcggccgg catcgagcgg gtgcccgacc tgatgaccag ggccaggccg
     2761 ccgaggagca gcaccgcagc cggcacctgc tcgagccgaa catgctgatg caagaacacc
     2821 gccgcgatca gcgtggcgcc cggcacttcg aacagaatcg acaggctgac gatcgttggg
     2881 ctcgtggtct tcagcacccg attgaagacg gagtggccga gcaactgagc accggcagtc
     2941 aaggccacga tgcgggccca atcagcaccg ctgtacccgt gcaagctctg gcgcccgata
     3001 aggcacacca cgaggagcaa cacggcggtt gtcgaatagc acagcgacgt atagctcgtg
     3061 gtcgtgacgg ttcgccggac agcagcgcct gcggtcacgt agcccgccgc caacatccca
     3121 ccaaccaatg ccagcaggtc gccactcaag gcccgcccgg acacccgaac gtcgagcccg
     3181 gtaagcagca cgaccgcgag caccgcgatg acgatccctg cccatgccga gcggccgatc
     3241 accgcgcctt gagtgcgggc aagaagggcc gcccaaacgg gctgggaggc gacgagcgcg
     3301 gtcgacgacg ccaccgatgt gtaacgaagg ctaggaatcc aggtggcgaa gtgtgccgcg
     3361 agcagcaccc cggcagtcac gatcagccgc cattcgcggc gcgtcaggcg tcgcagctcc
     3421 ggtcggtgcc gggtcaaggc gacggacatc atcgcggccg caccgagacc gttgcgccag
     3481 aacgcgagcg cgagggcggg cgctgccgac gccacgatca acggcccgga cgtcgacacc
     3541 gcgatcacgg cgatcgcgat cagcgtcagg tcggtactcg acggcagcgc caactcgtgc
     3601 gctggtccgg atgtcctctc gccctcgcgc attccggtca tgatgccagg accgcgaccc
     3661 agcgcaccgg acaccggcat cgggtcgggt gcacacttgg cgccatgacg acaggctcgg
     3721 gtacccgtga ccatcggtca cgtcgcgctg cctacgccct ggtgttcgtc gtggtggtct
     3781 tcgttgtcgt tcccgtcgtc gcgatgctcg ccgcctcgac ctgagcctcg tcgacgagcg
     3841 gcgtaaggtc gggctcgcta gcggaagtgg tcgaaaccgc ctgcggaata aggcaatcca
     3901 tcgacggtca ccgtgttctc gccggtgccg tcgagcacct cgccgatgac ctgccatccc
     3961 gcgggcagtt cggcaccggg cccaaatgtc gctaccaggg cgtggtcttc gccgccgcgc
     4021 aggatccagg tcagcgggtc gacgctcagc gccgacgcca cctgactcaa cgcgtcgggg
     4081 atggcgaatg ccgtcgaatc cacatcgatg cgcacgccgc tcgcagctgc gacgtggcca
     4141 aggtcggcga ccaggccgtc actcacgtcg atcatcgacg tggcgccggc cttcgccgcc
     4201 tcgggtccgg cctgatacgg cggttgcgga cgccgatggg cgtcgacgac cagccgcggc
     4261 gaccggaatc ccctgctcag cgtcgcaagg ccagccgctg cccacccgag ccgaccggcg
     4321 agcgcaacga catcgccgga ttgcgcaccg gacctggtga ccggcgtccg cccttcgaga
     4381 tcaccgagtg cagtgacggc gatcacgagc acatcgctgc gtacgacgtc tcccccgacg
     4441 acgcccgccc cgaccaggtc gcactcgtct cgcagcccgt cggcgaaacg ctcggcccag
     4501 aacgccgcaa ggtcagcagg cgcggcgagc ccgacaagaa gcgaagtcgt gaccgcaccc
     4561 atcgcggcaa cgtcagccag
//
LOCUS       DATCBJ010000121         3088 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_281578, whole genome shotgun sequence.
ACCESSION   DATCBJ010000121 DATCBJ010000000
VERSION     DATCBJ010000121.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 3088)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3088)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3088
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_281578"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            62..151
                     /locus_tag="VII50_02470"
     tRNA            62..151
                     /locus_tag="VII50_02470"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:96..98,aa:Ser,seq:cga)
     gene            483..620
                     /locus_tag="VII50_02475"
     CDS             483..620
                     /locus_tag="VII50_02475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5049739.1"
                     /translation="MGRTAAYRLVHDGQWPTPVLRLGRLIKIPTQPLLELLAGATRET
                     A"
     gene            634..777
                     /locus_tag="VII50_02480"
     CDS             634..777
                     /locus_tag="VII50_02480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5049740.1"
                     /translation="MQAGDRNRPLKPGWLIKIRTQPLLELFAGTPATSTFGVPAGGRK
                     LGS"
     gene            complement(1233..1901)
                     /locus_tag="VII50_02485"
     CDS             complement(1233..1901)
                     /locus_tag="VII50_02485"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEY5049741.1"
                     /translation="MPDPITARVEPTTPPAAAPAMSPDRHARGRERRRNRVYDAALAL
                     FMEKGFEETTMDDIAERADVARTSVFNYFSPKTAFLDEWGTRRREEALRAVESEHLED
                     ASVAKVLQRYMAVLGVLNADARAECVVMMGASLKWNNVVGGSLLAGALSDVLDQARLN
                     NQIREDIDIRQAGLLLAVGYFAALATWIAVEPEPFDIRDYLAKLVNLILNGLLESPPH
                     ASAD"
     gene            2048..>3088
                     /locus_tag="VII50_02490"
     CDS             2048..>3088
                     /locus_tag="VII50_02490"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY5049742.1"
                     /translation="MDENTGHPRRRAILAVMCLSLLIVVIDSTILNTALPTLARVLPA
                     STTDLQWITDAYSLVFAALLIVGGALGDRFGRRRALLVGLGIFGAGSIAAALSGDASM
                     LIATRAVMGLGAAFVMPATLSIITAVFPARERAGAIGAWSAVAGLGVVAGPSLGGLLL
                     DHFYWGSVFWLNIPLIAAAMVAAVLVVPDLAGKRSAGRLDILGAVLSAAGLVAIVDAV
                     IEGPDRGWGSVTTLVEVCAGALLIAAFIGRELRATNPLIDLRVFKHRAFSAATISVAL
                     TMFALFGSLFALTQYLQLVVGYSPLSAGLRALPFAAGVMVLAPVSSILVRAVGARVVV
                     PLGLTAMSAGLFI"
BASE COUNT          475 a          988 c         1002 g          623 t
ORIGIN      
        1 tgcggcgagc tgctccgagc gtttttcgag ggacgtcgag gggtcagcta ggctctcccg
       61 cggtggagtc gcgtagtggc cgagcgcgca cgcctcgaaa gcgtgtgagg gtgaaagctc
      121 tccgtgggtt caaaacccac ctccaccgcc accccgaaac gacgtttccg caggtcacag
      181 gcttgcggga cgccgtgaaa gtggccgaat tggcctagtg gtcacagaag tggtcacaga
      241 aaatttcgcg acgccgaaac tgaacggcgc gagggcgcac ttcgtcgcgc tgttcgagga
      301 cttcttgtcg acctgcagcg attaggacga cgtccctgag acgagtggcg ataccaacgc
      361 agcggcggct gcttgcatct cgagatcgac gtggctgtag gtgtcgaggg tgatgcccac
      421 gttcgcgtgg ccaagtcagg cttgaacgac gcggggatgt tcaggctgcc gcgctgctcg
      481 gtgtggggcg cacggccgcc taccgtctcg tgcacgacgg gcagtggccg accccggttc
      541 tgcggctcgg ccggctgatc aaaatcccga cccagccgct gctggagttg ctcgccggcg
      601 ctacccgcga gaccgcctga cgcgacgttg ggcatgcagg caggcgaccg aaaccggccg
      661 ctgaagcccg gctggctcat caagatccgg acccaaccgc ttctcgaatt gttcgccggc
      721 accccggcaa cgtcgacgtt tggggtgccg gcaggtggcc gaaaactggg cagctgatgc
      781 tcggccgaca ggtcaaaatc ccgacccgat ggctgctcga attgcttgcc tgcacccgcg
      841 gcacggtcgc cgaactgcgg cgctcggggc gccggcgacg gaccgaaacg ggctcgccga
      901 aactcgatca tttcatgggt tttgccgaat agaggatggt tgcagacgtg ccagcacttc
      961 tgtgcctggc gtggatgacg agtgggtcgt ggttcttgtc acgggtcgtt cgtttcgttc
     1021 cgcgcagaac gccatctgtg ctgtgcttcg gtttcctcgg tgtgacgctt ttgacttacg
     1081 cctccggggg caccactccg gttgccggag cggtcgcgct ggccgtgcag ggtttcgcca
     1141 tcttgggctt cacgcgtcga atacttttga gcggcatcat cgcgaccgac tctggcttgg
     1201 atctagcgga gccgtagcac atcaaggaca ggtcagtcgg ctgaggcgtg tgggggggac
     1261 tcgagtaagc cgttgagtat caggttcaca agtttcgcca ggtagtcgcg aatatcgaat
     1321 ggttctggct cgaccgcgat ccaggtagcc agcgcggcga agtatccgac ggcgaggagc
     1381 agtccggctt gtcggatgtc gatgtcctcg cgtatctggt tgttgagtcg agcctgatcc
     1441 aggacgtcgc tgagtgcgcc agccaggagc gagcccccca cgacgttgtt ccacttcagc
     1501 gacgcgccca tcatgacgac gcactcggcg cgagcgtcgg cgttgagcac gcctagtacg
     1561 gccatgtacc gctgcagaac cttggcgacg gatgcatctt cgaggtgctc ggactcgacc
     1621 gcgcggagag cctcctcccg ccggcgagta ccccactcgt ccaggaacgc ggttttgggc
     1681 gagaagtagt tgaaaacgct ggtgcgcgcg acgtctgccc gttcggcgat gtcgtccatc
     1741 gtggtctcct caaatccctt ttccatgaac agcgctagcg ccgcgtcgta gacccggttg
     1801 cgccttcgct cgcgccctcg ggcgtggcgg tccggtgaca tcgccggtgc tgccgctggc
     1861 ggcgtcgtcg gctctacgcg tgcagttatc gggtcgggca tcgtgccgcc tctttcatca
     1921 gatgaactca tttggattct aaaccaacat tgtacctgag tacgtcttgt acttggatcc
     1981 aaacttgtac taccgtagga acatccgagc gagggaccta accgcgccag cccactaagg
     2041 agtcaccatg gacgagaaca ccggacatcc acgtcggcgg gcgatcctcg ccgtcatgtg
     2101 cctatcgctg ctgatcgtgg tcatcgacag cacgatcctg aacaccgcgc tgccgacgct
     2161 ggcacgcgtg ctaccggcct cgaccaccga cctgcagtgg atcaccgacg cctactcctt
     2221 ggtcttcgcc gcgctactta tcgtcggggg cgccctgggc gaccggttcg gtcgccgacg
     2281 cgcgctactg gtcgggctgg gcatcttcgg cgccggctca atcgccgccg ccctgtctgg
     2341 cgacgcgtcg atgctgatcg ctacccgcgc cgttatgggt ctaggcgccg ctttcgtcat
     2401 gccggcaacc ttgtcgatca tcaccgccgt gttccctgcc cgagagcgtg cgggcgccat
     2461 cggcgcgtgg tcggcggtgg cgggcctcgg ggtcgtcgcc ggaccctccc tcggggggct
     2521 actgctggat catttctact ggggctcggt tttctggctg aacatcccgc ttatcgccgc
     2581 tgcgatggtg gccgccgtcc tcgtcgttcc tgacctggcc ggcaaacggt ccgctggccg
     2641 gctcgacatt ttgggggcgg tgttgtccgc tgctgggctg gtcgccatcg tcgacgcggt
     2701 gatcgaagga cccgaccgag gctggggttc ggtcacaacg ctcgtcgaag tctgcgccgg
     2761 tgcgctgctt atcgcagcgt tcatcggtcg ggagctgcgc gcaaccaacc cgctcatcga
     2821 cctcagggtg ttcaaacacc gggcgttctc agccgcgacc atctcggttg ctctcaccat
     2881 gttcgcgctg tttgggtcgc tgttcgccct cactcagtac ctacagctcg tggtgggcta
     2941 ctccccgcta tcggccgggc tgcgggcgct gcccttcgcc gcgggagtca tggtgctcgc
     3001 acccgtcagc agcattctgg tgcgcgccgt cggcgcacgc gtagtcgtcc cgctcggact
     3061 caccgcaatg agcgccggac tgttcatc
//
LOCUS       DATCBJ010000122         2750 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_244854, whole genome shotgun sequence.
ACCESSION   DATCBJ010000122 DATCBJ010000000
VERSION     DATCBJ010000122.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2750)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2750)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2750
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_244854"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..170)
                     /locus_tag="VII50_02495"
     CDS             complement(<1..170)
                     /locus_tag="VII50_02495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HEY5049743.1"
                     /translation="MAAGDTVITLIGNLVDDPELRFTPNGQAVAKFRIASTPRFMDKA
                     TNEWKDGESLFLT"
     gene            complement(173..463)
                     /gene="rpsF"
                     /locus_tag="VII50_02500"
     CDS             complement(173..463)
                     /gene="rpsF"
                     /locus_tag="VII50_02500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020134133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HEY5049744.1"
                     /translation="MRRYELMVLLDPDVEEKTVAPSLDQFLGVVRQDGGTVDKVDVWG
                     RRRLAYEISKKTEAIYAVIDIVAEPASVKELDRQLNLNETVLRTKLMRPEKH"
     gene            622..1407
                     /locus_tag="VII50_02505"
     CDS             622..1407
                     /locus_tag="VII50_02505"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009085231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HEY5049745.1"
                     /translation="MTTSLPKIGAHVDQDDPLAEARAREADLAQFFLGDPQDWKAPQI
                     RAGVEALKNAPIDLYVHSPYVINVATTNNRIRIPSRKILAQHADAAASIGAKALIVHG
                     GHVLKDDDPAVGVDNWRKTFERWTPPLPIFIENTAGGDNAMARHFDRLAMLWDAVGEY
                     GPGFCLDTCHAHAAGEDLVDVVARVKAITGRIDLVHANGSRDPFGSGADRHANFEEGE
                     IDPALIVEVVRQAGVPVVCETPNGATGQGADIAYLRKHLTADA"
     gene            complement(1411..>2750)
                     /locus_tag="VII50_02510"
     CDS             complement(1411..>2750)
                     /locus_tag="VII50_02510"
                     /inference="COORDINATES: protein motif:HMM:NF021134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase 87 family protein"
                     /protein_id="HEY5049746.1"
                     /translation="VLIAIALASSVLGYLQKYPCRSQPWTSGFQYSHVCYNDIYPLYF
                     GEGLSDGKIPYLDAHANDQGQLTGEVEYPVIIGAAMEVGSLATKALYPSNERGQGFID
                     LTWLMLTIAAVIAVIATALTNRRRIWDAAMLAAAPSLILAGLINWDLLAVALTSLGML
                     AWARKHNFLAGALLGLAIATKFYPIVFFLPLLLLCWRGRQLKAFWTAFGGAALAWAVV
                     DVPIWIYRPEGGFRFYYFSRTRGADWGSIWYVFSRPESTIVFHHEIHWFGWNYTVNQL
                     NLYEAAAIAIAFLGVCWLALAARRRPRLPQLCFLTLALFLVFNKVYSPQYVLWLLPLV
                     VLARPRWRAFLVWQAAEIIYFLGIWMYLLGDPSGRTGKGLEYGPYAISLAIRDISVLA
                     LCVLVIIDILRPDGDVVRADGSDDPAGGVLDGAPDIFDSEPSYEEPEPVMALN"
BASE COUNT          522 a          925 c          879 g          424 t
ORIGIN      
        1 gtgaggaaga ggctctcgcc gtccttccac tcgttcgtcg ccttgtccat gaaacgcggc
       61 gtcgaggcga tgcggaactt cgcgacggct tggccgttgg gggtgaatcg caactcgggg
      121 tcgtcgacca ggttgccgat cagcgtgatg acagtgtcgc cggcggccat ggctagtgct
      181 tctccggccg catgagcttg gtgcgcagca cggtctcgtt gaggttcagc tggcggtcga
      241 gctccttgac ggacgccggc tcagcaacga tgtcgatcac cgcgtagata gcctcggtct
      301 tcttcgagat ctcgtacgcc aagcggcgcc gaccccacac gtcgaccttg tccacagtgc
      361 cgccgtcttg gcgaacgacg ccgagaaact gatcaagaga gggggcgacg gtcttctctt
      421 ccacatcggg atcgagaagc accatcagtt cgtaacgacg catgcgtgca ccgacctcct
      481 tcggtctggg cggccacggt cgatccgtgg caggcgggct cgttgcgtcg aggcaacgcg
      541 tcacagtacc aagccagcgc ccccgacccg aaatcctgtc cacagccagc tcgcgaccgg
      601 cgtccggcct aaagtgaatc catgaccacg tcgctgccga agatcggtgc ccacgtcgac
      661 caggacgacc ccctcgcgga agcccgggcg cgcgaggccg acctcgcgca gttctttctc
      721 ggcgaccccc aagactggaa ggcgcctcag atccgggccg gcgtcgaggc tctgaaaaac
      781 gcccccatcg acctctacgt ccactccccg tacgtgatca acgtggccac gaccaacaac
      841 cggatccgaa ttccgagccg caaaatcctg gcccagcatg ccgacgccgc cgcaagcatc
      901 ggcgcgaagg cgctgatcgt gcacggcggc cacgttttga aggacgacga cccggcggtc
      961 ggcgtcgaca actggcgcaa gaccttcgag cgctggacgc ccccgctgcc gatcttcatc
     1021 gagaacacgg cgggcggcga caacgccatg gcgcgccact tcgatcggct cgcgatgctg
     1081 tgggacgccg tcggcgagta cgggcctggg ttttgcctcg acacctgcca cgcccacgca
     1141 gccggggagg atctggtcga cgtggtcgcg cgagtgaagg cgatcaccgg gcgcatcgac
     1201 ctcgtgcatg ccaatggctc acgtgacccg ttcggctcgg gcgccgaccg gcacgccaac
     1261 ttcgaggagg gcgagatcga cccggcgctc atcgtcgagg tcgtccgcca ggcgggcgtg
     1321 cccgtcgtct gcgagacgcc gaacggcgcc accggccagg gcgccgacat cgcctacctg
     1381 cgcaagcatc tcaccgcaga cgcctgagcc tcagttcagc gccatgacgg gctcaggctc
     1441 ctcgtagctc ggctcactgt cgaagatgtc gggcgcgccg tcgagcaccc caccggccgg
     1501 atcgtcgctg ccatcggccc gcaccacgtc gccgtccggt cgaagaatgt cgatgatcac
     1561 caggacgcac agcgccagca cggagatgtc cctaatggcg agggaaatcg catacggccc
     1621 gtactccagg cccttcccgg tgcggccgct cgggtcgccg agcaggtaca tccagatgcc
     1681 gaggaagtag atgatctcgg cggcctgcca gaccaggaat gctcgccacc gcggccgggc
     1741 gagcaccacg agcggcagca accacagcac gtactgcggc gaatagacct tgttgaacac
     1801 caggaagagc gccagcgtca agaagcacag ttgcggcagc cgtggccggc ggcgggcggc
     1861 gagtgcgagc cagcacacgc cgaggaaggc gatagcaata gcggcagcct cgtacaagtt
     1921 gagctggttc accgtgtagt tccagccgaa ccaatggatc tcatggtgga agacgatggt
     1981 tgattcgggt cgcgagaaca cgtaccagat cgagccccag tcagcgcccc gcgtccggct
     2041 gaagtagtag aaccggaacc ccccttccgg tctgtagatc cagatcggga cgtcgaccac
     2101 tgcccatgcg agcgcggctc ccccgaaagc ggtccagaag gccttcaact gccgcccccg
     2161 ccagcacaac agcagcaacg gcaggaagaa cacgatcgga tagaacttcg tcgcgatcgc
     2221 gagcccgagc agggcccccg ccaggaagtt gtgttttcgg gcccacgcca gcatgccgag
     2281 cgacgtcagt gcgaccgcca ggaggtccca gttgatcagc ccggccagga tcagcgaggg
     2341 cgcggcggcg agcatcgcgg cgtcccatat tcgacgccgg ttggtgagcg cggtggcgat
     2401 caccgcgatg accgccgcga tcgtgagcat cagccaggtc aggtcgatga agccctggcc
     2461 gcgctcattc gaggggtaca acgccttcgt cgcgagggag ccaacctcca tcgcggcccc
     2521 gatgatgact gggtattcga cctcgccggt caactgcccc tgatcgttgg cgtgggcgtc
     2581 gaggtaggga atctttccgt ccgacaggcc ctcgccgaag tacagcgggt agatgtcgtt
     2641 gtaacagacg tggctgtact ggaatccgct agtccaaggc tggctccgac acggatactt
     2701 ctgcaggtag cccagcactg agctggcaag cgcgatggcg atcagcaccc
//
LOCUS       DATCBJ010000123         7442 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_429487, whole genome shotgun sequence.
ACCESSION   DATCBJ010000123 DATCBJ010000000
VERSION     DATCBJ010000123.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 7442)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7442)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7442
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_429487"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(153..1328)
                     /locus_tag="VII50_02515"
     CDS             complement(153..1328)
                     /locus_tag="VII50_02515"
                     /inference="COORDINATES: protein motif:HMM:NF013697.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEY5049747.1"
                     /translation="MTNRTDLTADGTMAKYAADFNKIYPNVKVAFQGITNYEGDVKTR
                     MNTSNYGDVLLIPSALPISEYPTFFQPLGSATDLGAKYNFIANGTVNGQVYGIAQNGN
                     ANGFVYNKTVWAKAGITTWPTTPDQYVADLQAIKAKTTAIPYYTNYKDGWPLSSWEGA
                     VGSPSCSAAANDNLATETAPWTSGSDLFQIDSLLYNIVHNKLSEPDRTTTNWESSKGL
                     LATGKISAMWLGSWAVTQMQAAATTAGMDPSEIGFMPWPSETNGHLCSVIGPDYLQAI
                     NIHSSHKDAARAWIDWFTDQSTYAVDNGDVPTLKSGTQPPALAAYAAAGVTLVTLTQA
                     NSALVNKIDNTAEIGLTSPTYRQHIVDIARGAAGGSLQSDFASLNSKWAGSQKTGGA"
     gene            1336..1791
                     /locus_tag="VII50_02520"
     CDS             1336..1791
                     /locus_tag="VII50_02520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5049748.1"
                     /translation="MLPETVVGMVEPAADDAGALATAEAVAGEAVLAAAVVAGAALGA
                     LLEGVVVELAAVPLLLLHAATSEATTSSTPTEVFFTLTISISIRNPPPDTVGRPIGIK
                     RFSDRSSDTVERSATVVKVTTGSKRQSICNRYQHRTALTIERSAGIRPF"
     gene            1941..3041
                     /locus_tag="VII50_02525"
     CDS             1941..3041
                     /locus_tag="VII50_02525"
                     /inference="COORDINATES: protein motif:HMM:NF012742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEY5049749.1"
                     /translation="MIDGPSREPTVLTSRLVKGQVKRPTIADVARAAGVSQASVSFAL
                     NGQRGVSEETRRRVIAIAKEIGWNPNGAARALSGAASNTIGIALRRPARILGIEPFFM
                     ELISGVEAELSTRSYALTLQVVADQAAELAVYRRWWGERRVDGVLVCDVRVDDPRIHV
                     LEELALPTVVLGAAAGSRSLGNVWSDDAAAIVEAVEYLAALGHRRIARVGGLPELLHT
                     AIRTDAFADSCRRMGVEAAVTISSDYTGEEGARATRRLLSSADRPTAVIYDNDIMAVA
                     GLGVAHEMGLSVPGDVSLVAWDDSPLCQLVHPALTALSRDIPGYGAIAARQLLSVIAG
                     ESAESIQDEAPHLVTRGSTAAPALKAVKRAAK"
     gene            complement(3044..3271)
                     /locus_tag="VII50_02530"
     CDS             complement(3044..3271)
                     /locus_tag="VII50_02530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5049750.1"
                     /translation="MTDQTESKQRVQAEIDEIDQTLRSLRGERGTEVDDEGAQSDGAA
                     DLTNYEEEQALIDNLEARRRSLMEELDGRVA"
     gene            complement(3281..4168)
                     /locus_tag="VII50_02535"
     CDS             complement(3281..4168)
                     /locus_tag="VII50_02535"
                     /inference="COORDINATES: protein motif:HMM:NF014244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HEY5049751.1"
                     /translation="MADQQGQPGVEPGLVPDARRPYLYAPGELPGGLPPTPTRTKRRR
                     LEGAGGTAAAGGGVAAKFGLLTKLFLLLKASVVLVKFKFALSMAVSVIAYAFIFGWWY
                     AVGFVALIAVHELGHVVALRFMGIKASLPTFVPFMGAFVSMKSKPKSVAHEAVSALAG
                     PAVGTLGALAAFELSHVFNSPLLRALAYTGFFLNLFNLVPALPLDGGRVAGALHPALW
                     IVGLVAAGVFAILHPSPVLIFILVLGTVELVRRWRTHKWIDAAYYSIPPSTRLAIGSA
                     YAAVAVVCLWGMSLAYVRR"
     gene            4352..4840
                     /locus_tag="VII50_02540"
     CDS             4352..4840
                     /locus_tag="VII50_02540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5049752.1"
                     /translation="MATQTHDTPVVMADAVIAQVIQLKDPDEAISSAIWWRVAELLRA
                     CTTATASGPHLTTTLLEHAFNATDDVLSSLSADTDPSAAPSDLEALLPLDAALPLGAG
                     YDALAATRRSCLRVARLLFSLDATTPAMLATRVNQLRRALTAWRFEALDATVGRRISA
                     AH"
     gene            complement(4844..6037)
                     /locus_tag="VII50_02545"
     CDS             complement(4844..6037)
                     /locus_tag="VII50_02545"
                     /EC_number="2.3.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004565492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-acetyltransferase"
                     /protein_id="HEY5049753.1"
                     /translation="MPDGGDLIPASGGWREGDHPGDRRFAELGAFDLELGGQLPDVRV
                     AYETWGELNADASNAVLVLHALTGDSHVVGAAGPGHATAGWWDGLIGADAPIDSRKWF
                     VVAPNVLGGCQGSTGPSSLRTDGDPSRRTGGNRQWGSAFPRTTVRDQVTVEAALADRL
                     GISRWASLVGGSMGAMRVLEWLVEYPSRTSSALVLAVGATATGDQIGTQSAQLRAITS
                     DPRWRGGDYYDAADGEGPHEGMGLARRIAHLTYRSADELQVRFGNEPQDGEDPLRDGR
                     FAVQSYLDYQAVKLARRFDAGTYVALTDAMNTHDVGRGRGGVTAALSSITAPVIVAGV
                     DSDRLYPLVLQRALAAGIPTTVGGLRIVHSDVGHDGFLIERDAVGALVRETLAVADRV
                     LAGSR"
     gene            complement(6043..7338)
                     /locus_tag="VII50_02550"
     CDS             complement(6043..7338)
                     /locus_tag="VII50_02550"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009082886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional o-acetylhomoserine/o-acetylserine
                     sulfhydrylase"
                     /protein_id="HEY5049754.1"
                     /translation="MSDAWSFETKQIHAGQHPDEATSARALPIYQTTSYTFKNTDHAA
                     ALFGLAEFGNIYTRIMNPTQAVVEERVAALEGGVAALLVASGQSAETLAILNIASAGD
                     HIVSSPSLYGGTYNLFHYTFPKLGIEVSFVEDPDDPQSWRDAVRPNTKAFFGESLANP
                     KSDFLDIETVAAIAHENGVPLIIDNTVPTPYLLRPFEWGADIVVHSATKYLGGHGTAI
                     AGLIVDGGRFDWAAHADKFPGLTTPDPSYHGVVYTEAVGNLAYIIKARVQLLRDLGPA
                     IAPFNAFLIAQGLETLSLRIERHVANATKVAQWLEQHDEVERTIYAGLPSSPWYERGQ
                     KYSPRGAGAIVSFEIKGGLEAGKRFVNALELHSHVANIGDVRSLVIHPASTTHSQLSP
                     QEQLATGVTPGLVRLAVGIEGIDDILADLDLGFRAAKEA"
BASE COUNT         1252 a         2440 c         2439 g         1311 t
ORIGIN      
        1 ttctcccggc accaaatgcc cgaccccggt gcccgtggtg tccgtcatct gtttccctct
       61 tgccgctgcc gggggttgaa cggggttggg cggcgggtct tgacgagctc ccgccgccca
      121 acaccttgcg tcctatcaat cacgccctac cgctacgcgc cgccggtttt ctgcgagccc
      181 gcccacttgc tgttcaggct ggcgaagtcg ctttggaggc taccgccggc cgctccgcga
      241 gcgatgtcga cgatgtgctg acggtaggtc gggctggtca gaccgatctc cgcagtgttg
      301 tcgatcttgt tcaccaatgc cgagttggcc tgggtgagcg tgaccaacgt gacgccagcg
      361 gctgcgtacg ccgcgagtgc cggcggctgg gttccactct tcagcgtcgg gacgtcgccg
      421 ttgtcgactg cgtaggtcga ctggtcggtg aaccaatcga tccatgctcg tgccgcgtcc
      481 ttgtggctgg agtggatgtt gatggcctgc aggtagtcag gaccgatgac cgagcacagg
      541 tgcccgttag tctccgatgg ccagggcatg aaaccgatct ctgacgggtc catccctgcc
      601 gtcgtcgcag ctgcctgcat ctgcgttacg gcccacgagc ccagccacat cgcggagatc
      661 ttgccggtcg cgagcaagcc cttcgagctc tcccagttgg tcgtggtccg gtccggctca
      721 ctcagcttgt tgtgcacgat gttgtagagg agcgagtcga tctggaagag atcgctaccc
      781 gaggtccacg gcgcggtctc ggtagccagg ttgtcgttgg cggcagcgct gcagctcggg
      841 gagccaaccg caccttccca cgaactcaac ggccaaccgt ccttgtagtt ggtgtaatac
      901 ggaatcgccg tggtcttcgc cttgattgct tgcaggtcgg cgacgtactg gtcaggcgta
      961 gtcggccaag tcgtgatgcc ggccttcgcc cacacggtct tgttgtagac gaaaccgttc
     1021 gcgttgccgt tctgcgcaat gccgtacacc tggccattga cggtgccgtt cgcgatgaag
     1081 ttgtacttgg caccgagatc agttgcagat cccagcggct ggaagaacgt cgggtattcc
     1141 gaaatgggca acgcgctcgg gatcaacaga acgtcaccgt agttcgacgt gttcatccga
     1201 gtcttgacgt caccctcgta gttcgtgatg ccctggaagg ccaccttgac gttggggtag
     1261 atcttgttga agtcggccgc gtacttcgcc atggtcccgt cagcggtcag gtccgtgcgg
     1321 ttggtcaaga ccgtgatgct gccggagacg gttgtcggaa tggtcgagcc ggcggccgat
     1381 gatgccggag cgctcgccac cgcggaggcc gttgcggggg aagcggtgct ggcagccgcc
     1441 gtggttgcag gcgccgcgct aggggcgctg cttgagggtg tggtcgtcga gctcgcggcg
     1501 gtccctttgc tgctgctaca tgccgcgacg agcgaggcga ccacgagcag cacccccact
     1561 gaggtcttct tcacactgac catctccatt tcgattcgca atcccccgcc cgatacagtg
     1621 gggaggccaa ttgggattaa gcgttttagc gaccgatcga gcgacacggt agagaggtcg
     1681 gccaccgtgg tcaaggtcac aacagggtcc aagcgccagt cgatatgcaa ccgttaccag
     1741 caccgcactg cgctgacaat tgagcggtcc gcgggtatcc gtccgttcta aagacgctcc
     1801 cgggagcacg gcctcgaggc ggtaggtgcg cgatcgcggc tcgacaccag gctcgtggcc
     1861 gccgggtact tgcacactga ctactaaacc agttaagtaa tcgtgtcatc tggcatcaca
     1921 gctggcggcg accgggcgtc atgatcgacg gtccgagccg cgagccaacg gtgctaactt
     1981 ctcgtctcgt gaagggtcaa gtgaagcggc ccaccatcgc tgacgtggca cgcgcggctg
     2041 gggtctcgca ggcctccgtt tcctttgcgc tgaacggtca gcgaggcgta tccgaagaga
     2101 cgcggcgtcg tgttattgcc atcgccaaag agatcggctg gaaccccaac ggcgcagccc
     2161 gcgcgctttc aggcgcggcc agcaacacga tcggcatcgc gctacggcgg ccggcccgga
     2221 tccttggcat cgagccgttc ttcatggagt tgatcagcgg cgtcgaggcc gagctttcca
     2281 cccgctccta tgcgctcacg ctccaagtgg tcgccgatca agccgccgaa cttgccgtct
     2341 accgccggtg gtggggcgag cggcgagtcg acggcgtcct cgtctgcgac gtccgagtcg
     2401 acgacccacg aatccatgtc ctcgaggagc ttgcgttgcc gacggtggtg cttggcgcag
     2461 cagcgggcag ccgcagcctc ggcaacgtgt ggtcggacga cgccgccgca atcgttgagg
     2521 ctgtggagta cctcgccgcg ctcgggcatc gcaggatcgc gcgtgtcggt gggctccctg
     2581 agctgctgca tacggcgatc cgtaccgacg ccttcgctga ctcatgccgg cggatgggcg
     2641 tcgaggcggc cgtcaccatt tcgtccgact acaccggcga ggagggtgcg cgcgcgactc
     2701 gccggctgct gagttcggcg gatcgaccga cagcggtgat ctacgacaac gacatcatgg
     2761 ccgtcgccgg cctcggcgtc gcgcacgaga tgggcctgtc cgtacctggc gacgtatcgc
     2821 tagtggcgtg ggacgactcg ccgctctgcc agctcgtgca tcctgcgctg accgcgctgt
     2881 cgcgcgacat tcccggttac ggcgccatcg ccgctcggca gttattgtca gtgatcgccg
     2941 gcgagtcggc cgagtcgata caggacgaag caccacacct ggtgacgcgc ggcagcaccg
     3001 ccgcgcccgc actcaaagct gtgaagcggg ccgccaagtg acgtcaagct acccggccgt
     3061 ccagctcctc cattaacgat cggcggcggg cctcgaggtt gtcgatcaac gcctgttcct
     3121 cttcgtagtt ggtcagatct gcggcgccat cggattgggc gccctcatcg tcgacctcgg
     3181 taccgcgctc accgcgaagg ctgcgcagtg tctgatcgat ctcgtcgatc tcggcctgca
     3241 cccgttgctt gctctcggtc tgatcggtca ccgatctctc ctaacggcgg acgtaggcga
     3301 ggctcatgcc ccataggcag accacggcga cggcagcgta tgcgctaccg atggcgagcc
     3361 gcgtcgacgg cggaatcgag tagtacgccg cgtcgatcca cttgtgcgtt ctccaacgcc
     3421 ggaccagctc gaccgttccg agcacgagga tgaagatgag tactgggctc gggtgcagga
     3481 tcgcgaaaac gcccgcggcc accagcccga cgatccagag tgcgggatgc agcgcaccgg
     3541 cgacccggcc gccgtccagc ggcagcgccg gcaccaggtt gaagaggttg aggaagaacc
     3601 cggtgtaggc cagcgcccgc aacagcggcg agttgaagac gtgcgagagc tcgaacgcgg
     3661 ccaaggcacc gagcgtgccg acggccgggc cggcaagcgc gctcaccgcc tcatgcgcga
     3721 ccgacttcgg tttcgacttc atcgagacga acgcacccat gaacgggacg aaggtcggca
     3781 gcgacgcctt gatacccatg aagcgcagtg ccacgacgtg cccgagttca tgcaccgcga
     3841 tcagcgcgac gaaaccgacc gcgtaccacc agccgaagat gaatgcgtag gcgatgaccg
     3901 agacggccat gctgagcgcg aacttgaact tgaccagcac cacactggcc ttgagcagca
     3961 gaaagagctt ggtcagcagt ccgaactttg cggccacgcc gccaccggcc gccgcggtcc
     4021 cacccgcacc ctccaaacgg cgtcgcttcg tgcgcgttgg cgtaggcggc aggccgccgg
     4081 gcagctcgcc cggcgcgtaa aggtacggcc gccgagcgtc tggcactagc ccaggctcga
     4141 cgcccggttg gccttgctgg tcagccacgg tgctcgcaac tccactattc gacgatcttc
     4201 agtgtaagtg agcgcggcgc cgcacgctgc ccgcttcaaa cccttgtaga cgaacgccaa
     4261 tactgccagg aagttcccag caacagcgca gggagctttc agtctccagg accagagtga
     4321 gcccaagacc tactcagcga ctggagctcc gatggccaca cagacgcatg acactcccgt
     4381 agtgatggcg gacgccgtga tcgcacaagt aatccagttg aaggaccctg acgaggccat
     4441 ctcgagtgcg atctggtggc gggtggctga gttgctgcgg gcatgtacca ccgctacggc
     4501 cagcgggcca cacctgacga ccaccctgct cgagcatgcg ttcaacgcga ccgacgacgt
     4561 gctctcctcg ctcagcgccg acaccgaccc gagcgccgcg ccgtccgatc tcgaggctct
     4621 cttgccgctc gatgccgcac tcccccttgg cgccggttac gacgccctcg cggcgacccg
     4681 gcgcagctgc ttgcgggtgg cccggctgtt gttttcgctc gacgccacga cgccagcgat
     4741 gctcgccaca agagtcaacc agttgcgccg cgcgctgact gcctggcgat tcgaagcgct
     4801 cgacgcaact gtcgggcgcc ggatcagcgc ggcgcactaa aggtcagcgg ctgccggcca
     4861 gcacccgatc ggcgacggcg agcgtctctc tgacgagtgc cccaacggcg tcgcgctcga
     4921 tgaggaagcc gtcgtgtccg acgtcggaat gcacgatgcg cagacccccg acggtcgtcg
     4981 gaatcccggc tgcgagtgcg cgctgcagca ccagcggata gagccgatcg gagtcgacac
     5041 cggcaacgat caccggagcc gtgatggagc taagcgcggc tgtcacgcct ccgcgcccgc
     5101 gaccgacatc gtgggtgttc atcgcatcgg ttaacgcgac ataggtcccg gcgtcgaacc
     5161 gccgcgctag cttgaccgct tgatagtcga ggtaggactg cacggcaaaa cggccgtcgc
     5221 gcaagggatc ttcaccgtcc tgcggctcat tgccgaaacg cacttgcagt tcgtcggcac
     5281 tgcggtaggt gaggtgggca atgcgacgcg cgaggcccat tccctcgtgc ggtccctctc
     5341 catcggcggc gtcgtaatag tcaccgccgc gccagcgcgg atcactcgta atggcacgca
     5401 gctgcgcgct ctgggtgccg atctgatcgc cggttgcggt cgcgccgacc gcaagaacca
     5461 gggcggacga cgtgcggctg gggtattcga caagccactc gagcaccctc atcgcgccca
     5521 tcgaaccgcc gaccagcgac gcccacctcg agatgcccag gcgatcagcg agcgccgcct
     5581 cgacggttac ctggtcgcgg accgtggtgc gcggaaaagc cgatccccac tggcgattcc
     5641 cgccggtgcg ccgggatgga tccccgtcgg tgcgcagcga cgacggcccg gtgctgccct
     5701 ggcagccacc gagaacgttc ggcgccacca cgaaccactt gcggctgtcg atgggtgcat
     5761 cggcgccgat cagtccatcc caccaaccgg cggtggcatg acccgggcct gcggcgccga
     5821 ccacgtgcga gtcgcccgtg agggcatgca gaaccagcac cgcgttcgag gcgtcggcgt
     5881 tcagctcgcc ccacgtctcg tacgcgacgc ggacatccgg cagctgcccg ccgagctcga
     5941 ggtcgaaggc cccgagctcg gcgaagcgcc tgtcacctgg gtgatctccc tcgcgccacc
     6001 cgcccgaggc ggggatgaga tcaccgccgt ctggcaccgg gctcaggcct ccttcgcggc
     6061 gcggaagccc aggtcgaggt cggcgaggat gtcgtcgata ccttcgatgc cgacggcgag
     6121 ccggaccagg cccggtgtga cgcccgtcgc taactgctcc tgcggagaca gctgcgagtg
     6181 cgtcgtggac gccgggtgga tgaccagaga ccggacgtcg ccgatgttcg ccacgtgact
     6241 atgcagctca agcgcgttga cgaatcgctt tcccgcctcg agaccgccct tgatctcgaa
     6301 cgagacgatg gcgccggcgc cacgcggcga gtacttctgc ccgcgctcgt accacggcga
     6361 cgacggcagg cctgcgtaga tggtccgctc gacctcgtcg tgctgctcga gccactgcgc
     6421 caccttcgtc gcgttggcaa cgtgccgctc gatgcgcagc gacagggttt ccaggccctg
     6481 ggcgatcagg aacgcattga acggtgcgat cgccggaccg aggtcgcgca gcaactgcac
     6541 ccgcgccttg atgatgtacg ccaggtttcc gaccgcctcg gtgtagacaa ccccgtggta
     6601 gctcgggtcg ggcgtcgtca ggccggggaa cttgtcggca tgcgctgccc aatcgaagcg
     6661 gccaccatcg acgatgagcc cggcaatcgc tgtcccgtgc ccgccgaggt acttcgttgc
     6721 ggagtgcacg acgatgtcgg cgccccactc gaacggccga agcaagtagg gcgtcggcac
     6781 cgtgttgtcg atgatcagcg gtacgccgtt ctcgtgcgcg atcgccgcca ccgtctcgat
     6841 gtcgaggaag tcgctcttgg ggttggcaag ggactcgccg aagaaggcct tcgtgttggg
     6901 ccgaacggcg tctcgccatg actgtggatc atcgggatcc tcgacgaacg agacctcgat
     6961 gccgagcttg ggaaatgtgt agtggaagag gttgtaggtg ccgccgtaca ggcttggact
     7021 cgagacgatg tggtcgccgg cgctggcgat gttcaggatg gcgagtgttt cggccgactg
     7081 gccactggcg accagcagtg cggcgacgcc tccctcgagc gccgctaccc gctcctctac
     7141 gacggcctgc gtcgggttca tgatgcgcgt gtagatgttg ccgaactccg ctagtccgaa
     7201 gagcgccgcc gcgtggtcgg tgtttttgaa ggtgtaggag gtcgtttggt agatgggcag
     7261 cgcccgcgcg ctagtggcct cgtccgggtg ctggccggca tgaatctgtt tggtctcgaa
     7321 actccaggcg tcgctcatgg gattcctctc gggcaaattg gaagacatag gtgtggatcg
     7381 gacgaagcga acggtgctgg ctcgaagccg gccggcacgg tctgttgcgc tggtcagcag
     7441 ta
//
LOCUS       DATCBJ010000124         2375 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_503114, whole genome shotgun sequence.
ACCESSION   DATCBJ010000124 DATCBJ010000000
VERSION     DATCBJ010000124.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2375)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2375)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2375
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_503114"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            191..1300
                     /locus_tag="VII50_02555"
     CDS             191..1300
                     /locus_tag="VII50_02555"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEY5049755.1"
                     /translation="MKASGAALAILAATETITRAAADDASAQLGLVLCLLALATTVPL
                     IFLRPVASVLAISVASVLSAVPFHSLTVAGAFAQLFATYRLGRGDGGVIAALVFVPFP
                     VLWFADPGHGEDRVLTILLASLIPAAAWAGAATQSRSAALAHTAAQAVIAGTSFEYAA
                     RGERARIARELHDVVAHHISMVAVQAETARLTTPGMPDAGARRLSAIGDTARAALTEM
                     RRLLGVLREDADSDPVASRRPQPGLGELNELLDAAREASGSGTRLIVYGSPVALDPGV
                     ELAAYRIAQEALTNARRHAPGAAVDVELRYADEILCLRVRDNGPGPPLAAPDGAHGLL
                     GMGERAAAVGGRLRFGAAAGGGFLVEATLPARATT"
     gene            1300..2004
                     /locus_tag="VII50_02560"
     CDS             1300..2004
                     /locus_tag="VII50_02560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009085703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEY5049756.1"
                     /translation="MNAAVEHVRIVVADDHEVVRAGFAELLDTRPDFSVVGIASDGAE
                     AVRLCRDLQPDVVLMDVRMPGMDGIEATRRLAELGPIAPRVIILTTFDLDEYVYEALR
                     AGASGFLLKDVTAERLFEAVRVVADGEALLAPGVTRRLIGEFAALRRRSNLDAVGALG
                     PAGLEVLTPRETQVLRLVAEGLSNAEIAARLVVNEETVKTHVSRVLRKLGLRDRTQAV
                     VTAYESGLVVPHRRRY"
     gene            2145..2345
                     /locus_tag="VII50_02565"
     CDS             2145..2345
                     /locus_tag="VII50_02565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5049757.1"
                     /translation="MLKQDDVIAALDHARIADGPGGPAEMVPGQHHVRMNMYIGQAEN
                     GRFKTVESLGVIDPHERTLATA"
BASE COUNT          374 a          765 c          812 g          424 t
ORIGIN      
        1 gatgaccgac ccgaatcgct tgcgcaggcc atttaccgcg atagtggctt ccatggccag
       61 caggctaggt accggcgata gtcgcggtcg tcaccctggc gagccatctt cgccgtcccc
      121 cacaggaggg acatcgctgt cacctacgag agggacgcct gaacggctgc acaccgcgac
      181 actcatcggt gtgaaagcga gcggggccgc gctggcaatt cttgcggcga ccgagacgat
      241 cacgcgtgcc gccgcggacg atgcatccgc acaacttggc ctcgtgctgt gcctgctcgc
      301 gttggcgacc acggtgccgc taattttctt gcggcccgtt gcgtccgtgc ttgccatctc
      361 ggtggcgagc gtgctttcgg cggtgccgtt tcacagcctc acggtcgcag gcgcgttcgc
      421 gcagttgttc gccacttacc ggctcggccg cggcgatggg ggagtgatcg ctgcgctggt
      481 tttcgtgccg ttccccgtgc tctggtttgc cgatcccgga cacggcgaag atcgggtcct
      541 cacgatcctg ctggcgtcac tgataccggc agcggcttgg gccggcgccg cgacgcagtc
      601 gcgaagtgcg gcgctggcgc acaccgctgc acaagcggtg atcgccggca cgtcattcga
      661 gtacgcggcc cgcggtgaac gcgcgcgcat cgctcgcgag ttgcacgacg tggtcgctca
      721 ccacatctcg atggttgccg tgcaagctga aaccgcgcgt ctgacgacgc cagggatgcc
      781 ggatgctgga gcccgtcggc tttctgcgat tggtgacacg gcgcgtgcgg cgctgactga
      841 aatgcggcgg ctgctcggcg tcctgcgcga agacgcggat agcgacccgg tcgcttctcg
      901 gcgtccgcaa ccggggcttg gagaattgaa cgaactcctt gacgccgcgc gcgaggcgtc
      961 gggatcgggc acccggctga tcgtgtatgg ctcgccagtc gccctcgatc ccggcgtcga
     1021 acttgccgcc taccgaatcg cccaggaggc gttgacaaac gcgcgaaggc acgcgcctgg
     1081 cgccgctgtc gacgtcgaat tgcgttacgc cgatgagatc ttgtgcttgc gcgttcgcga
     1141 caacggaccg gggccgcctc tggcggcgcc ggacggtgct catgggctcc tcggcatggg
     1201 cgaacgtgct gccgcggtgg gtggccgatt gcgattcgga gctgccgcgg gcggagggtt
     1261 cctcgtcgag gccaccctgc ctgctagggc gacgacctga tgaacgccgc tgtcgagcac
     1321 gtgcgcatcg tggtcgccga cgaccacgag gtcgtccgcg ccggattcgc cgagttgctc
     1381 gacacgaggc ctgatttcag cgtggtcggc attgcgtccg acggtgccga ggccgtgcgc
     1441 ctgtgccgcg atctacagcc cgatgtcgtg ctgatggatg tgcgtatgcc gggcatggac
     1501 ggcatcgaag caacccggcg gctcgcggag ttagggccga tcgcgccgcg cgtcatcatc
     1561 ttgaccacct tcgatctcga tgaatacgtc tacgaagcgt tgcgtgcagg cgcgagcggg
     1621 ttcttgctca aggacgtcac cgctgagcgc cttttcgagg ctgttcgggt ggtagcggac
     1681 ggcgaagcac tgcttgcacc cggcgttact cgccgcctga tcggcgagtt cgcagcgcta
     1741 cgtcgcaggt cgaacctcga cgctgttggc gcgctgggac ctgcagggct agaggtcctg
     1801 acgccgcgcg aaactcaggt gttgcggctg gtggccgagg gtctctccaa cgccgagata
     1861 gcagcgcggc tcgttgtcaa cgaggagacc gtcaagaccc acgtgagtcg cgtactgcga
     1921 aagctcgggc tgcgcgacag gacccaagcg gttgtcaccg cgtacgaatc agggcttgtc
     1981 gtgccgcacc ggagacggta ctgaagccga ccccttcagt cgggacctac tcgacaagta
     2041 caaccgccga ttccctggca gcgccaagtt cgccgctggg agcgcctgca ctgggctcta
     2101 ccgcgggctg cggctgtggc aggcagcggt caactaggcg ccgcttgctc aagcaggacg
     2161 atgtcattgc cgcgctcgac catgcacgta tcgccgacgg gcctggtggc ccggcggaga
     2221 tggtgccagg acagcaccac gtccggatga acatgtacat cggccaggcc gaaaacggcc
     2281 ggttcaagac cgttgaaagc ctgggtgtca tcgatccgca cgagcgcacg ctcgcgaccg
     2341 cctgagcaac cagtggaggt cggctcgcat gagtc
//
LOCUS       DATCBJ010000125         2579 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_133740, whole genome shotgun sequence.
ACCESSION   DATCBJ010000125 DATCBJ010000000
VERSION     DATCBJ010000125.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2579)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2579)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2579
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_133740"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..387
                     /locus_tag="VII50_02570"
     CDS             <1..387
                     /locus_tag="VII50_02570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5049758.1"
                     /translation="MVDVPPSCRVDVNADPTLLNGVQLMGGEPRARYLYLREGALDDV
                     LAAWRECLGDRAVVCVRDEAIDAGWFGPVTDRYRARIGDVVVAALADIAVVDSRRHPR
                     EARLLGHHGSVTAAEQLVPLLAFGSG"
     gene            396..1241
                     /locus_tag="VII50_02575"
     CDS             396..1241
                     /locus_tag="VII50_02575"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase"
                     /protein_id="HEY5049759.1"
                     /translation="MTPRPELLVDVVSLASELAGDRPPLLIDVRWSLTGPPGIEAYRA
                     GHLLGARFADLDKDLAGRRGAGGRHPLPEPADFEGFMQRLGVRSDSAVVVYDAADGVS
                     AARAWWDIRYFGHHDIRVLDGGYAAWEAAGLPVTTDEPPLVPGDFVARPGGLPVLDAD
                     AAARLAVNGVLIDVRAGERFRGEVEPVDPIAGHIPGAVNLPTSGNVGPDGRFLDGRTL
                     ARRFESVDGGDAEQVGAYCGSGVNAAHTAFAMTLAGVPTPALYVGSWSNWITDGTRAI
                     ATGAD"
     gene            complement(1261..>2579)
                     /locus_tag="VII50_02580"
     CDS             complement(1261..>2579)
                     /locus_tag="VII50_02580"
                     /inference="COORDINATES: protein motif:HMM:NF024180.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HEY5049760.1"
                     /translation="SLGATDLRVHATGLGSDLAGQGVRLAVESGKPGAVLDWIERFRA
                     GTLRRKPVRPPADARLARELAQLRRVTAELAEVTAAGTDSRALRAEQLQLEDAVRHRS
                     RHARGSRTAVREFDADQVLAALGDRALVEILRVDDALFAVTGTDGRLRLHQLGAYPDA
                     MRELESVRFSLHRLARRHGSPASLAAANAGLTHATSALDGLLVEPLLDVVGDRELVIV
                     PTGQLHALPWPTLPSLRGRPVVVAPSATTWLAAMRAASPSRNNANRRVAFVAGPHLDH
                     AEDEVRAVAQLYRKPEVLAGPGATADDVRQTMDGAELVHIAAHGRFRADNPQFSAIEL
                     ADGPLTVYDLERVHRGPRRLILSACDSGLSAVHAGDELMGLAGAVFSLGTSTLIASVV
                     PVADDLTKALMVELHRELRTGATPAHALATAQLRVQVDGFVCFGAA"
BASE COUNT          394 a          845 c          921 g          419 t
ORIGIN      
        1 atggtcgacg ttccaccgag ctgtcgcgtc gacgtcaacg ccgacccaac cctgctcaac
       61 ggcgtgcagt tgatgggtgg cgaacctcga gcccggtatc tgtacctgcg cgagggcgcg
      121 ctggacgacg tgctcgcggc atggcgtgaa tgcctcggtg accgagcggt ggtctgcgtg
      181 cgcgacgagg cgatcgacgc gggctggttc ggtccagtca ccgatcggta ccgcgcgagg
      241 attggtgatg tcgtggtcgc cgcgctcgcc gacatcgctg tggtcgactc acgtcgccac
      301 ccgcgcgaag cacggctgct cggccaccac ggatcggtga ccgcggcaga gcagctcgtt
      361 ccgctcctgg cattcggcag cgggtgacgc actcgatgac gccacgtccg gagctgcttg
      421 tcgacgtcgt gtccttggcg tcagaactgg ccggtgatcg gccgccgctg ctgatcgacg
      481 tccgatggag tttgaccggg ccgcccggga tcgaggccta ccgcgccggc cacctcctgg
      541 gcgcgcggtt cgctgacctc gacaaagact tggccgggcg ccgaggggct ggcggccgcc
      601 atccgcttcc cgaacccgcg gatttcgaag gcttcatgca acgcctcggt gtccgcagcg
      661 atagcgcggt tgtcgtgtac gacgccgcgg acggcgtgtc ggccgcgcgg gcttggtggg
      721 acatccggta cttcggccat cacgacattc gggttcttga cggtggatac gcggcctggg
      781 aagcggctgg gctcccggtg accacagacg agccgccgct cgtgcctggc gatttcgtgg
      841 ctcgcccagg tggactgcct gtgctcgacg ccgacgcggc cgcgcggcta gcggtcaacg
      901 gggtgctgat cgacgtccgg gctggagagc gctttcgggg tgaagtcgag ccggtcgatc
      961 cgatcgccgg gcacattccc ggcgccgtca atctgccgac gagcggcaac gtcggtcccg
     1021 acggcaggtt tcttgatggg cggaccttgg cgagacgctt cgagtccgtt gatggtggcg
     1081 acgcggaaca ggtcggcgcc tattgcggca gtggtgttaa tgctgcacat acggcgttcg
     1141 caatgaccct cgccggcgtg ccgacacctg cactgtatgt cggctcgtgg tcgaactgga
     1201 tcaccgacgg gacgcgcgct atcgcaacag gcgcggactg atcgacaacg gacgcggcgc
     1261 ttacgcagcg ccgaagcaga cgaatccgtc gacctgcacc cggagttgtg cggtcgcgag
     1321 cgcgtgagcg ggcgttgcgc ccgtgcgtag ctcgcgatga agttcgacca tcagagcctt
     1381 cgtgaggtcg tcggccacgg gcaccaccga cgcgattaat gtgctggtac ctaaggaaaa
     1441 gacggcaccc gccaggccca tgagctcgtc gcctgcgtgg accgcagaca gtcctgagtc
     1501 gcaggccgaa agaatgagcc ggcgcggtcc tcgatgcacc cgctcgaggt cgtacaccgt
     1561 cagcggcccg tccgcgagtt cgatcgcgga gaactgcggg ttgtctgccc ggaagcggcc
     1621 atgagccgcg atgtgcacca actccgcgcc gtccatggtt tgccggacgt cgtcagcggt
     1681 cgcccccgga ccggccagca cttcgggctt gcggtagagc tgggcgactg cccgtacctc
     1741 gtcttcggcg tggtcgaggt gcgggcccgc gacgaaggcg acgcgcctgt tggcgttgtt
     1801 ccggctaggg ctggccgcac gcattgctgc caaccacgtc gtggccgacg gtgccacgac
     1861 caccggtcgg ccccgcagcg agggcagcgt cggccaaggc agggcgtgca gctgcccggt
     1921 cgggacgatg acgagctccc ggtcgcccac cacgtcaagc agcggctcta ccagcagacc
     1981 gtcgagcgcc gacgttgcat gggtgaggcc ggcattggcg gcagccaacg aggcgggcga
     2041 gccgtggcgg cgagccagcc ggtgcaacga aaagcgcacc gattcaagtt cgcgcatcgc
     2101 atcggggtag gcgcctagtt ggtgtagtcg gagtcggcca tcagtgccgg tcaccgcgaa
     2161 gagcgcatca tcgacccgga ggatttcgac aagagcccgg tcgccgagtg cggcgagtac
     2221 ttgatcggcg tcgaactcgc ggacggctgt gcggcttccc cgcgcgtgcc gcgatcggtg
     2281 ccggaccgca tcttcgagtt ggagttgttc ggctcgcaac gcgcgcgaat ccgtgcccgc
     2341 ggcggtgacc tccgcgagtt cggcggtgac gcgtcgcagc tgcgccagct cgcgggccaa
     2401 tcgagcatca gccggcggcc ggaccggctt gcggcgaagc gttccggccc ggaaacgctc
     2461 tatccaatcg agaaccgcgc ctggttttcc cgactcaacg gccagccgga cgccctgacc
     2521 ggcgaggtcg gagccgagac cggtggcgtg cacccgaagg tcggtggcgc cgagcgacg
//
LOCUS       DATCBJ010000126         2251 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_429420, whole genome shotgun sequence.
ACCESSION   DATCBJ010000126 DATCBJ010000000
VERSION     DATCBJ010000126.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2251)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2251)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2251
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_429420"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..299
                     /locus_tag="VII50_02585"
     CDS             <1..299
                     /locus_tag="VII50_02585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5049761.1"
                     /translation="QLNPVFRIGSRAVAWPWAALGRNALVVYVGQHVLGAVLAQTPAH
                     VDTQLTTAAGYLQEHFFAGGWLGLDSQWTYVAVMLAFWTAVAAAMHRARWYVTL"
     gene            333..932
                     /locus_tag="VII50_02590"
     CDS             333..932
                     /locus_tag="VII50_02590"
                     /inference="COORDINATES: protein motif:HMM:NF028007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HEY5049762.1"
                     /translation="MSAYLEIWGRRGPQLAPLDTDHVTVGRGAENTVSLSWDRTVSAL
                     HAVIERYASGWSVRDLGSTNGTFVNNSRLSAEHRLRNGDEIRFGSSRLVFRLRNAPAS
                     TATAAAEPPPHITRREQEVLVVLCRPLLGGDMFTEAATVATIAADLVVTEAAVKFHLG
                     NLYDKFDISDGGDGVRRSRLANEAIRRRALTLSDLRPPT"
     gene            929..>2251
                     /locus_tag="VII50_02595"
     CDS             929..>2251
                     /locus_tag="VII50_02595"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEY5049763.1"
                     /translation="MSEMWRGRYGSVETIAQGGQGRLLRGVDRQHDRPVALKVRNVAS
                     TEERNHVLAEARVLLSLRPHPGLPTVREDFFEGDSYVLVMDWVDGTSLDQVVAARGAP
                     GMPLPSVLRWATQLAAAIDHLHAHRPVIVHGDVKPANAVLTPDDRVVLVDFGIATNGV
                     ATRSGTVGYAAPEVLDGQAPTAAADIYGLAATLLALLTGRPPGDVRPPWDGLDPITIE
                     VIERGLRPGLALEPDRRPANARELVERMRAWAGPAAEVVEPSVRHGGGTPDAHVRKGS
                     RAASRWWRRPVSFPAWLRVSVVAVVVAAIVVGFAFGSKPRSPVRRVQGPDRYSTAAQV
                     ALNTFTHANTAILARGDDLADAIAGSYLAGAQGGGPILLTPPDGLQVDVMHALSQLKV
                     KQVFIIGDTTAIAASVDNALQAKGMRTTRLAGVTRYATAGVIEQAGGQA"
BASE COUNT          362 a          774 c          727 g          388 t
ORIGIN      
        1 gacaactcaa tccggtattc cggatcggat cacgcgcggt tgcgtggccg tgggcggctc
       61 tcgggcgtaa tgctttggtc gtctacgtcg gtcagcacgt acttggcgcc gttctcgcgc
      121 agaccccggc gcacgtcgac acgcaactga ctactgcggc cggctacttg caggagcact
      181 tcttcgcggg tggctggctc ggtctcgact cgcagtggac ctacgtcgcc gtgatgcttg
      241 cattttggac ggcggtcgct gccgcaatgc atcgcgcccg ctggtatgtc accctctaac
      301 ccaatggata ctgctattgc aggttgcgcc ccgtgagcgc gtacctagaa atatggggac
      361 gccgcgggcc gcagctcgct ccactcgaca ccgaccacgt cactgtcggg cgtggtgcgg
      421 aaaacaccgt gtcgctctcg tgggaccgca ccgtctcggc gttgcacgcc gtgattgagc
      481 gctacgcctc gggctggtcg gtccgcgatc tgggttccac caacggaaca ttcgtcaaca
      541 acagtcggct ctcggccgag caccggctgc gtaacggtga cgagatccgg ttcggctcca
      601 gtcgcttggt cttccgactg cgcaacgccc ccgcttcgac agcgacggct gccgccgaac
      661 cgccaccgca catcacgagg cgcgagcagg aggtgctcgt cgtgctatgc cgcccgctgc
      721 tcggcgggga catgttcacc gaagccgcaa ccgtggccac catcgcggcg gatctggtcg
      781 tcactgaagc cgcagtcaag ttccaccttg gcaacctcta cgacaagttc gacatcagcg
      841 acggaggcga cggcgtccgc cgatcgcggc tggccaatga ggcgattcgc cgtcgagcgc
      901 tcaccctctc ggacttgcga ccgcccacat gagcgagatg tggcgcgggc ggtacggatc
      961 tgtcgagacg atcgcgcaag gcggtcaggg caggctgctt cgtggggttg atcgccaaca
     1021 cgatcggccg gtcgccctca aggtacgcaa cgtcgcctcc accgaggaac gcaaccacgt
     1081 cctcgccgag gctcgggtgc tgctcagctt gcggccacac cccggcctgc cgaccgtacg
     1141 ggaggacttc ttcgagggcg acagctacgt gctggtcatg gactgggtcg acggcacttc
     1201 cctggatcag gtcgtcgcgg ctcgaggcgc accgggaatg cccttgccca gcgtgctgcg
     1261 ctgggcaacg caacttgccg cagcgatcga ccacctgcat gcgcaccggc ccgtcatcgt
     1321 gcacggtgac gtcaaacctg ccaatgcggt gctgacccct gacgaccggg tcgtgctcgt
     1381 cgacttcggc atcgcgacca acggagtcgc gaccagatcg gggaccgtcg gctacgcagc
     1441 cccagaggtc ctcgacgggc aggcgccaac ggcggcagcc gacatttacg gtctcgcagc
     1501 cacgctgctc gcactgctga caggtcggcc acccggcgac gtccgacctc cgtgggacgg
     1561 ccttgacccc atcacgatcg aggtcattga acgtggcctg cgacccggac tcgcgctcga
     1621 gcccgatcgc cgcccggcga atgcacgcga actcgttgag cggatgcgcg cgtgggccgg
     1681 tcccgccgcc gaggtcgttg aaccgtcggt acgtcatgga ggcggtacac ctgacgcgca
     1741 tgttcgcaag gggtcacgtg ccgcctcgcg ctggtggcgg cgtcccgtgt cttttccggc
     1801 gtggttgcgg gtgtccgttg tcgcggttgt tgtcgccgcc atcgtcgtcg gcttcgcgtt
     1861 cggcagcaaa ccgcgctcgc ccgtgcgtcg cgttcaggga ccggaccggt actcgaccgc
     1921 tgcgcaggtg gcgttgaaca cgttcacgca cgccaacacc gcaatcctcg cccgaggcga
     1981 cgatctcgcc gacgcgattg ccggaagtta ccttgccggc gctcaaggcg gcggcccgat
     2041 cctgctgact ccaccggacg gcctgcaggt cgatgtcatg catgcgttga gccaactcaa
     2101 ggtgaagcag gtcttcatca tcggcgacac gacggccatt gctgccagcg tcgacaacgc
     2161 actgcaagcg aaagggatgc gcacgacccg gctggccggc gtcacgcgct acgccaccgc
     2221 cggtgtcatc gaacaggccg gcggacaggc c
//
LOCUS       DATCBJ010000127         2806 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_286523, whole genome shotgun sequence.
ACCESSION   DATCBJ010000127 DATCBJ010000000
VERSION     DATCBJ010000127.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2806)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2806)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2806
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_286523"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            45..782
                     /locus_tag="VII50_02600"
     CDS             45..782
                     /locus_tag="VII50_02600"
                     /inference="COORDINATES: protein motif:HMM:NF019028.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030527 - structural constituent of
                     chromatin [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histone H1-like repetitive region-containing
                     protein"
                     /protein_id="HEY5049764.1"
                     /translation="MPAKVVLRKGRTGKFTFTLEGPTGQILATSADHPNLRAAKAAIA
                     SVQKNLSGANVDDRSASTTVAKKTTTRKAAAPRKAAAKKTTAARKTAAKKTTVRKTAA
                     KKTTARKAAPRKAAAKKTTVRKTAARKTTAARKTAAKKTTARKTAAKKATARKTAAKK
                     TTARKAAPRKAAAKKTTVRKTAARKTAAKKATARKTTARKTTARKAAPRKAAVKRTTV
                     RKAAPRKAAAKKTTARKAAPRKRVTAS"
     gene            970..1842
                     /gene="map"
                     /locus_tag="VII50_02605"
     CDS             970..1842
                     /gene="map"
                     /locus_tag="VII50_02605"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009647818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HEY5049765.1"
                     /translation="MQPLDASDRGVLRPGVQTARRDVPPAIERPYYVDESRWQQRSEV
                     EAKDADTIAAMRVAGRLAAQALAEVGRSIVPGVTTDELDRIGHEFLCDHGAYPSTLGY
                     RGFPKSLCASLNEVICHGIPDSTEAVEGDIINIDITAFIGGVHGDTNATFMVGEVEDG
                     SRLLIERTREALDRAIHAVSPGRSFNVIGRVIESYAKRFGYGVVRDFTGHGIGTLFHS
                     GLIVPHYDVPEYDLPMMTGMTFTIEPMLTLGTVEYDVWPDGWTIVTKDRARSAQFEHT
                     ILVTDSGAEVLTLP"
     gene            1915..2748
                     /locus_tag="VII50_02610"
     CDS             1915..2748
                     /locus_tag="VII50_02610"
                     /inference="COORDINATES: protein motif:HMM:NF018649.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1295 domain-containing protein"
                     /protein_id="HEY5049766.1"
                     /translation="MAVFSWTTMGWASLAALGGVVIVVAVTFLTAQRVGRHNVIDTAW
                     GLGFLVALLAADAVAIGSRHHGGTDRRILITTLTALWAVRLAIHMGLRSRGKGEDPRY
                     VAMLADAPGSRTLYALRKVYLTQALAMWFVSLPLQVTAVRGGSIGLLGWLGVAVWIVG
                     FVFEAGGDWQLGAFRNDPANAGLVMDRGLWRYTRHPNYFGDAAQWWGFALIAFAHWPG
                     ILTITSPALMTWFLARKTGKPLLESHLTASRPGYREYIEATSGFFPLPPRSRTNPSVA
                     S"
BASE COUNT          544 a          967 c          881 g          414 t
ORIGIN      
        1 gacgtcccgg tcgcagaccg gaaccatctg ccgaggaggc atgagtgccg gcgaaggttg
       61 tattgcgaaa gggccggacc ggcaagttca cgttcactct cgaaggcccc accggccaga
      121 tcctggctac gtcagccgac caccccaacc tacgagctgc aaaggcggcg attgcatccg
      181 tgcaaaaaaa cctgtcaggc gccaacgtcg acgatcgcag tgcatcgacg acggtcgcca
      241 agaagaccac cacgcgcaag gctgcggccc cgcgtaaggc ggccgcgaag aagacgaccg
      301 ctgctcgcaa gaccgcagca aagaagacca ctgtccgcaa gaccgcagcg aagaagacca
      361 ctgctcgcaa ggccgctccc cgcaaggcag cagccaagaa gacgaccgtc cgtaagactg
      421 cggcccgcaa gacgaccgca gccagaaaga ctgccgcgaa gaagaccact gctcgcaaga
      481 ccgcagcgaa gaaggccacc gctcgcaaga ccgcggccaa gaagaccacc gctcgcaagg
      541 ccgctccccg caaggcagca gccaagaaga ccacggtccg caagaccgca gctcgtaaga
      601 ctgcggccaa gaaggccacc gcgcgcaaga cgactgctcg caagaccacc gcgcgcaagg
      661 cagctccccg caaggcagcg gtcaagcgga cgaccgtccg taaggccgcc ccgcgcaagg
      721 cagcagccaa gaagacaacg gcccgtaagg cagctccgcg caagcgcgtt accgcgagct
      781 agtaccgcga catagaacga ccgcgacata gaaggaccgt tacacgaatg gcagacggca
      841 cgacgtcacg acgaggcggc gagctcccac gcaccatcgg gagccgccgc tgcgtcaatt
      901 ttgcgggacg caatctgtgg cactgccgga agcaatctgt caaccgtgcg ggcacataga
      961 ctgacctgca tgcagccgct ggacgcaagc gatcgaggcg tgctgcgccc cggcgtccag
     1021 acggcccgtc gcgacgtccc accggcgatt gaacggccct actacgtcga cgagtcacgc
     1081 tggcagcagc gcagtgaggt cgaggccaag gacgccgaca cgatcgccgc catgcgagtc
     1141 gcgggccgcc tcgcagcgca ggccctggcc gaagtcggcc gctccatcgt ccctggcgtg
     1201 accaccgacg agctcgatcg catcggtcac gagttcctgt gcgaccacgg agcctacccg
     1261 tcgacgctgg gctaccgtgg ctttccgaag tcgctctgcg cgtcgctgaa cgaggtcatc
     1321 tgtcacggca tccccgactc gacggaggcg gtcgagggcg acatcatcaa catcgacatc
     1381 accgcattca tcggcggcgt ccacggcgat acgaacgcga cgttcatggt cggcgaggtc
     1441 gaggacggat ctcggttgct catcgaacgc actcgagagg cactcgaccg ggccatccac
     1501 gcagtcagtc ccggtcggtc gttcaacgtc atcggccggg ttatcgaaag ctatgccaaa
     1561 cgcttcggtt atggcgtggt ccgcgatttc accggacacg gcatcggcac gctcttccat
     1621 tcgggcctca tcgtcccgca ctacgacgtg cccgaatacg acctgccgat gatgacgggc
     1681 atgacgttca ccatcgagcc gatgttgacc ctcggcacgg ttgagtacga cgtgtggccg
     1741 gacggctgga ctatcgtgac caaagaccgt gcccgcagcg cccaattcga acatacgatc
     1801 ttggtgaccg actccggggc cgaagtcctc acccttccct gaagggcaca taagaagcgc
     1861 gtgcccgcct gcgccgggca atccggcgtg gccctgccta acgaacaggc gggtatggcc
     1921 gtgttttcgt ggacaacgat gggttgggca tccctagccg cgctcggcgg tgtggtgatc
     1981 gtcgtcgccg tcacgttcct gacagcacag cgggtcgggc ggcacaacgt catcgacacc
     2041 gcctgggggc tcggttttct ggtggcgctg ctggccgccg acgcggtcgc gatcggctcg
     2101 agacaccacg gcggcacgga ccggcgcatt ctcatcacca ctctgaccgc cctctgggca
     2161 gtgcggctag ccatccacat gggcctgcgc agccgaggca agggtgagga tccgcggtac
     2221 gtcgcgatgc tcgccgacgc acctgggagc cgcacgctct atgcgttgcg caaggtgtat
     2281 ctgacccagg cgctggcgat gtggttcgtt tcgctgccgc tccaagtaac cgccgtccgc
     2341 ggcggatcga tcggtctcct gggctggctc ggcgtcgccg tctggatcgt cggcttcgtt
     2401 ttcgaggccg gcggcgactg gcagttgggt gcgttcagga acgacccggc caacgccggc
     2461 ttggtcatgg accgaggcct gtggcgctac acacgccatc cgaactactt cggcgacgcc
     2521 gcccagtggt ggggcttcgc gctgatcgcc tttgctcact ggccgggcat cctcaccatc
     2581 acctcccccg cgctcatgac gtggttcctg gctcgcaaga ccggcaagcc gttgctcgaa
     2641 tcgcacctca cggcgtcgcg gcccggctac cgcgagtaca tcgaggccac cagcggcttc
     2701 ttcccgcttc cgccacgctc acggaccaac ccgtcggtcg cgtcgtgacc gggacgacga
     2761 ggcgttcggt cgcggtcgtc ggcgccggag tgtccgggct gacggc
//
LOCUS       DATCBJ010000128         2066 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_133663, whole genome shotgun sequence.
ACCESSION   DATCBJ010000128 DATCBJ010000000
VERSION     DATCBJ010000128.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2066)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2066)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2066
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_133663"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..310)
                     /locus_tag="VII50_02615"
     CDS             complement(<1..310)
                     /locus_tag="VII50_02615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5049767.1"
                     /translation="MSRREAGAGLPWVPFRAAPPPLRDAPAPRTKQPAREQIWREIRI
                     GVRRYHRLLRAGMIAIAVAIVMAQLSPKPAPTAAIVAAARDLPAGAVLTAADLRTSQF
                     P"
     gene            complement(307..1122)
                     /locus_tag="VII50_02620"
     CDS             complement(307..1122)
                     /locus_tag="VII50_02620"
                     /EC_number="2.4.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011718997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017061 - S-methyl-5-thioadenosine
                     phosphorylase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5'-thioadenosine phosphorylase"
                     /protein_id="HEY5049768.1"
                     /translation="MIDDFSSPIGVIGGSGFYEFVDDPVSVPVDTPFGHPSEPVVTGL
                     VQGRPVAFIPRHGRDHRYPPHRIPYRANLWALRSVGVRQVLAPCAVGSLRADLGPGSL
                     VVPDQVVDRTWGREFTYYDTVGPVVHVGFADPYCPHGRAAVVEVATAQSWEPHDGGTL
                     VVIQGPRFSSRAESAHHTAQGWSVVGMTGQPEAMLARELALCYTSIAVVTDLDAGVEA
                     GEGVSHQEVLAAFAASISRLRSLLFGVIAALPTERTCDCSHALDGQPLPFALP"
     gene            1201..1506
                     /locus_tag="VII50_02625"
     CDS             1201..1506
                     /locus_tag="VII50_02625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5049769.1"
                     /translation="MNAQELAAVFAGVFPPFDFGVELGFASALGFASALGFAEAPDSP
                     DLVSAAGLLAESPESADPDPEPEPEPVSELEPEPDSVDDPDPPVSTRAFESLRLSVR"
     gene            complement(1569..1661)
                     /locus_tag="VII50_02630"
                     /pseudo
     CDS             complement(1569..1661)
                     /locus_tag="VII50_02630"
                     /inference="COORDINATES: protein motif:HMM:TIGR02605.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FmdB family zinc ribbon protein"
BASE COUNT          356 a          704 c          703 g          303 t
ORIGIN      
        1 gggggaattg cgacgtgcgc aggtctgcgg cggtgagcac ggctcccgca gggaggtcgc
       61 gcgccgcagc aacgatggcc gctgtcggtg cgggcttggg cgacagttgc gccatgacga
      121 tcgcgaccgc gatcgcgatc atgcccgcgc gcaacagccg gtgatagcgg cgcacgccga
      181 tgcggatctc gcgccagatc tgctcgcggg cgggttgttt ggtgcgcggc gccggggcat
      241 cgcgaagcgg tggtggggct gcgcgaaacg gcacccacgg gaggccggcg cctgcttcgc
      301 gccggctcac ggcaaggcga atggcagcgg ctggccgtcg agcgcgtggg agcagtcgca
      361 ggtgcgttcc gtcggcaacg cagcgatcac accgaacagc agcgatctca gccgcgagat
      421 gctcgcagca aacgcggcga gaacctcctg gtgactcacg ccttcaccgg cctcaacccc
      481 ggcgtcgagg tcggtcacca ctgcgatcga ggtgtaacag agggccagct ctcgggccag
      541 catcgcctcc ggctgcccgg tcatgccgac cacggaccag ccctgagccg tgtgatgcgc
      601 agactcagcc cgactcgaga accgcggacc ctggatcacc accagcgtgc cgccgtcgtg
      661 cggctcccac gactgcgccg tcgccacctc gacgacggcc gcgcggccat gagggcagta
      721 cggatcggcg aaaccgacgt gcacgacggg accgaccgtg tcgtagtacg tgaactcgcg
      781 cccccacgtg cggtcgacga cctggtcggg gaccacgaga ctgcccggcc cgaggtcggc
      841 gcgcagcgac ccgaccgcgc acggcgcgag cacttgccgc acgcccaccg agcgcagcgc
      901 ccagaggttc gcgcgatagg gaatccgatg cggtgggtag cgatgatcac gcccgtggcg
      961 tgggatgaac gcgaccggcc ggccctggac cagccccgtg accaccggct cgctcggatg
     1021 gccgaacggg gtgtcgaccg gcacgctcac ggggtcgtcg acaaactcgt aaaaccccga
     1081 gccgccgatc accccgatgg gcgacgaaaa gtcatcgatc atgcgcagac cctaggtgat
     1141 gccgctgcag atcggacgac ggctcaagca gatgggggac gccgaattgc tggcctgtgg
     1201 atgaacgctc aggaactcgc ggcggtcttc gcgggggtct tcccgccgtt cgacttcggc
     1261 gtcgagctcg gcttcgcgtc ggcactcggc ttcgcgtcgg cactcggctt cgccgaggcg
     1321 cccgactcac cagacttggt ctccgccgcg ggcttgctcg ccgagtcgcc ggaatccgcc
     1381 gaccctgacc ccgagccgga gccggagccg gtgtccgagc tcgaacccga accagactcc
     1441 gtcgatgacc ccgacccccc ggtgtccact cgtgcttttg aatcgcttcg actgtcggtg
     1501 cggtagaaac cggagccctt gaacacgacg ccgaccgacg agaagacctt gcggagcttg
     1561 ccgccgcacg tcgggcacac ggtgagcgcg tcgtcagtga acttctggac ggcctccagc
     1621 ggcgtgctgc actcggtgca cacgtactga tacgtcggca cgtcttagac ctccgctagc
     1681 actctcgacc cttgattgct aatgatgagg catccaccag cgacctacaa gtttgcgcac
     1741 cggcgtcacc cgcactgccg tcgtccaatc gaagatgcac ggcggcaggc tacctgcgag
     1801 gggctggcgc tgctggtttg cccggcgtag cgtccgaggc tcgtgaggtg actccgtcgc
     1861 ctcaagcccg agcgagctgg agagcagcca tggccgaagc gatcatcttg gagttcaaag
     1921 gcgtcggcaa gaacgagtac gaccaggtca acaagattct cgggaccgac tattcgaccg
     1981 gtgagggcgc ctggcccgac gggctgctga accacaccgg cgcggtcacg gcaaacggtg
     2041 gcctcgtcgt gtacgagatc tgggag
//
LOCUS       DATCBJ010000129         2190 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_502905, whole genome shotgun sequence.
ACCESSION   DATCBJ010000129 DATCBJ010000000
VERSION     DATCBJ010000129.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2190)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2190)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2190
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_502905"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..237)
                     /locus_tag="VII50_02635"
     CDS             complement(<1..237)
                     /locus_tag="VII50_02635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5049770.1"
                     /translation="MTTRFGFVSTYPPTICGLATFTSSLFNELVTSAGNEGHVIRLVE
                     TPQPQAGPEVVGQLVAGDPRSAYRAAELLNDGDIA"
     gene            768..1916
                     /locus_tag="VII50_02640"
     CDS             768..1916
                     /locus_tag="VII50_02640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5049771.1"
                     /translation="MATTGTITAALGAFALAPAAQASEPNHTGQRDAERAVFVQTNDP
                     AGNAVIAYGRGDDGSLHWAGQYATGGLGGSESGAVVDPLASQGSLTYDARDHLLFAVN
                     AGSDTFTVFDVNGTHLHRLQVVGSAGHLPTSISVVASHVYVLDAGNDGTISGFWIDAA
                     RHLNAIGGSTRVLGLGNPADPNFLASPSQVAITPDGRDVIVATKTHGVIDVFALDHLG
                     VPSAAPVVTTSAGGVPFGLSYDTLGRLIVAEASGGASSYWVHPSGALETISSHVANAQ
                     AATCWSVIARGFVYTANAGSNTITGYAENWHGQLSLLNADGITATTDAGPVDLAASSD
                     GRYIYQEATGAGVIDEFKVNGNGSLTPIGTVTGFTPDNGTGFEGIAAS"
     gene            complement(1945..>2190)
                     /locus_tag="VII50_02645"
     CDS             complement(1945..>2190)
                     /locus_tag="VII50_02645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5049772.1"
                     /translation="LAQAAVHAAMAGRTAMVVGRWHGRFVHLPITLATASRNQVDPDG
                     DLWMSVLEATGQPPRFGRPTVQLPAERNPLAPSMAAG"
BASE COUNT          400 a          708 c          712 g          370 t
ORIGIN      
        1 ggcgatgtcg ccatcgttga gcagctcggc ggcgcggtac gcactgcgcg ggtcgccggc
       61 caccagttgc ccgacaactt ccgggccggc ctgcggctgc ggcgtctcga cgaggcgaat
      121 gacgtgaccc tcgttgcctg ctgacgtcac cagctcgttg aacaaggacg acgtgaaagt
      181 ggcgagacca caaatggtgg gcgggtacgt actgacgaat ccgaagcgag ttgtcatgat
      241 cgcagccttc cggttaccgg cgacgcgacg cgtgcgcgat gtttgcacgc gtgcgaggtg
      301 gaaattgccg cagcagagtt agtgatcaag aagcccccac ccgagtcggg cgaacagggc
      361 gatcatgtgc tccgcggggc gaagcgatga ccacagccta ccgtgccccc agcgctcggg
      421 ccctaatcgt cgacggggaa taactgctga cagcgctccc ggctcgggtt cgatcttcaa
      481 gacgccgcca agggctgggt gcgaccttgc gcgatggtgg accaccggcg cgcacatcga
      541 gggagattct tacgaactca tgacggcccg ttcttacaga ttgctgacgc caggccgttt
      601 ttgcttccac ggggctgacg cggtcgaaaa ccactgctag aaagtcgttt cgtgacccct
      661 gaggccacga gtccgacgga cgagccgtcg aatcagcgat tggaattcac atggcacgaa
      721 cggtccgcag aagtggcgca cgacacctga cccggcacct cacggtgatg gcaacgacgg
      781 gaaccatcac cgcggcgctc ggagcattcg ctctcgctcc agccgcccaa gcgagtgagc
      841 cgaatcacac tggacagcgg gacgccgagc gcgccgtatt cgtgcaaacc aatgatccgg
      901 cgggcaatgc ggtgattgct tacggtcgcg gagacgacgg cagcctgcac tgggccggtc
      961 agtatgccac cggcggtctt ggcggttcgg agtccggtgc tgtcgttgac ccgctcgcct
     1021 cgcagggttc gctgacctac gacgctcgcg atcacctgtt gttcgccgtc aacgctggca
     1081 gtgacacgtt cacggtgttc gacgtcaacg gtacgcattt gcatcggcta caggtcgtcg
     1141 gcagtgccgg ccatttgccg acgtccatca gcgtcgtggc tagccatgtg tacgtgcttg
     1201 atgcaggcaa cgacggcacg atcagcggct tctggatcga tgccgcgagg cacctgaatg
     1261 cgatcggcgg ctcgacccgc gtcctcggcc tgggtaaccc tgccgacccg aacttcctcg
     1321 cgtctccctc gcaggtcgcg atcactcctg acgggcgcga cgtgatcgtt gccaccaaga
     1381 cccatggcgt catcgacgtg tttgcgctcg atcacctcgg cgtgccgtcg gctgcgcccg
     1441 tcgtgacgac gtcagctggc ggcgtgccgt tcggcctcag ctacgacaca ctcggtcgat
     1501 tgatcgtggc cgaagcaagc ggcggtgcat cgtcgtactg ggtgcacccg agcggggcgc
     1561 tcgagaccat ctcgtcgcac gttgccaatg cccaagcggc aacctgttgg tcggtcatcg
     1621 cgagaggctt cgtctacacg gccaacgccg gctccaacac gatcaccggg tacgccgaga
     1681 actggcacgg ccagctcagc ctgctgaatg cggacggcat caccgcaaca acagacgcgg
     1741 gaccggtcga cctcgctgcg agtagcgatg gtcgatacat ctaccaggaa gcgaccggcg
     1801 cgggcgtcat cgacgagttc aaggtcaacg gcaacgggtc tctgacgccg atcggcaccg
     1861 tgaccgggtt cacgcccgac aacggcaccg ggttcgaagg aatcgccgca tcctgagaag
     1921 aagtgcgcgg gcatggcggt ctgactagcc agccgccatg ctcggcgcca aaggattgcg
     1981 ctctgccggc aactgcaccg taggtcgacc gaagcgcggt ggctgaccgg tcgcctccag
     2041 cacggacatc cacaaatcgc cgtccggatc gacctgattg cggctcgcgg tggcgagtgt
     2101 gatcggcagg tgcacgaacc ggccgtgcca gcggccgacc accatggccg tgcggccggc
     2161 catcgcagcg tgtacggccg cttgggcgag
//
LOCUS       DATCBJ010000130         2483 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_73655, whole genome shotgun sequence.
ACCESSION   DATCBJ010000130 DATCBJ010000000
VERSION     DATCBJ010000130.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2483)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2483)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2483
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_73655"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(34..1314)
                     /gene="eno"
                     /locus_tag="VII50_02650"
     CDS             complement(34..1314)
                     /gene="eno"
                     /locus_tag="VII50_02650"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018225678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HEY5049773.1"
                     /translation="MAAIEALGAREILDSRGNPTIEVEALLDDGTIARAGIPSGASTG
                     AFEAAELRDGGQRYGGKGVEKAVQAVIDDIAPEVIGFEASEQRLLDAKLIELDGTPNK
                     SKLGANAILGVSLAVARAAAESAELPLFRYLGGPNAHLLPVPMMNIVNGGSHADSNVD
                     IQEFMIAPIGAGTFAEAFRWGAETYHALKSVLKARGLSTGLGDEGGFAPNLGSNREAL
                     DLILEAIAKAGFTPGRDIALALDVAATEFHKDGTYAFEGGSKSASDLIAYYTDIVANY
                     PLVSLEDPLDEEDWDGWKAITDALGSKVQIVGDDLFVTNPIRIARGIASGTANALLVK
                     LNQIGTLTETFDAVDMAHRAGYRTMVSHRSGETEDTTIADFVVATNAGQIKSGAPARG
                     ERIAKYNQLMRIEEELDDAARYAGASAFPRFVVG"
     gene            complement(1353..2009)
                     /locus_tag="VII50_02655"
     CDS             complement(1353..2009)
                     /locus_tag="VII50_02655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG family protein"
                     /protein_id="HEY5049774.1"
                     /translation="MAGRRGERFLDLVEVMDRLRSPGGCPWDAEQTHATLVKYLLEEA
                     YETAEAIEDGDDGALREELGDVLLQVVFHARIAQDDAGFDIDDVAAGIADKLVRRHPH
                     VFAGAQANTAGDVEANWEALKAVEKGRTSAADGVPLAQPALALAAALVGRASKAGLDV
                     PVPGAIKAPDVVDEESIGALLMAVVVLARTAKVDPEGALRAAARRYRGLVQAAEGAAR
                     "
     gene            complement(2029..2427)
                     /locus_tag="VII50_02660"
     CDS             complement(2029..2427)
                     /locus_tag="VII50_02660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5049775.1"
                     /translation="MTQIIRDDLIADAAKAKGVTVTEAEIQSLLAQERQTNGSDEATA
                     KANGIPFAGLHTAVYQALLLNKLITALAPGQTDQTAQSAALTSYLGTIAKQEGVSVNP
                     RYGAWQSVQFDVVASDAFTSPASGSPAPTG"
BASE COUNT          370 a          839 c          816 g          458 t
ORIGIN      
        1 cgtcgtctgc ggccggaccg cggcggggca acgtcagccg accacgaaac gggggaacgc
       61 cgacgcacct gcgtagcgcg ccgcatcgtc gagttcttcc tcgatgcgca tcagctggtt
      121 gtacttcgca atgcgttcgc cgcgcgccgg tgcgccactt ttgatctgcc cggcattggt
      181 ggccaccacg aagtcggcaa tcgtggtgtc ctcggtctcg ccggatcggt gcgaaaccat
      241 cgttcggtag cccgcccgat gcgccatgtc cacggcgtcg aaggtctcgg tcaaggtgcc
      301 gatctggttc aacttcacca gcagcgcatt cgcggtgcca ctcgcaatcc cccgtgcgat
      361 gcgaatcgga ttggtcacga acaagtcgtc gccgacgatc tgaaccttcg agcccagcgc
      421 atcggtgatg gccttccagc catcccagtc ctcttcgtcg agcgggtctt cgagcgatac
      481 cagcgggtag ttcgcgacga tgtcggtgta atacgcaatc aggtcggacg ccgatttgct
      541 gccgccttcg aatgcgtacg tgccgtcctt gtgaaactcg gtcgcggcga cgtcgagggc
      601 gagcgcgatg tcgcggcccg gagtgaatcc agccttcgca atcgcctcga ggatcaggtc
      661 gagtgcttcg cggttgctgc cgaggttggg cgcgaaccca ccctcgtcgc cgagcccggt
      721 cgacagaccg cgagccttca gcaccgactt caacgcgtgg tacgtctccg cgccccaccg
      781 gaatgcttcg gcgaacgtgc cggcgccgat cggagcgatc atgaattctt ggatgtcgac
      841 gttggaatcg gcgtgggatc cgccgttgac gatgttcatc atcggcaccg ggagcaggtg
      901 cgcgttgggg ccgccgaggt agcggaacag tggcagctca gcggattcgg cggcggccct
      961 ggccaccgca agtgacacac cgagaatcgc gttcgcaccg agcttggact tgttgggcgt
     1021 gccgtcgagt tcgatcagct tcgcgtcaag cagtcgctgc tcactggctt cgaaaccgat
     1081 gacctcggga gcgatgtcgt cgataactgc ctgcacggct ttctcgacgc ccttgccgcc
     1141 atagcgttgc ccaccgtcac gtagttcggc ggcctcgaat gcacccgtcg aggcgccgct
     1201 cggtatccct gcgcgcgcga tcgtgccgtc gtcgagtagg gcctcgactt cgatggtcgg
     1261 gttgcctctg gaatcgagga tttcgcgagc cccgagggct tcgatggcgg ccacagtggt
     1321 actcctgagc atctggttgg cgggccgtca gtttaccggg ccgcgccttc agcggcctgc
     1381 accaacccgc ggtagcggcg ggctgcggcg cgcaatgctc cttcgggatc gaccttcgcg
     1441 gtgcgtgcca gcacgacgac ggccatcaac agagcgccga ttgactcctc gtcgacgaca
     1501 tcgggcgctt tgatcgcacc aggtaccggg acgtcgagtc ccgccttcga cgcacggccg
     1561 accagcgcgg ctgcaagcgc aagagcgggc tgcgccagcg ggacgccgtc agctgccgac
     1621 gtccggccct tttccaccgc cttcaaggct tcccagttcg cctcgacgtc gcccgcggtg
     1681 ttcgcctgcg cccccgcgaa gacgtgcggg tggcggcgga cgagcttgtc ggcgatgccc
     1741 gcggccacgt cgtcgatgtc gaaacccgcg tcgtcctggg caatgcgtgc atggaacacg
     1801 acctggagca acacgtcgcc gagctcttcg cgcagcgcgc cgtcgtcgcc gtcctcgatc
     1861 gcctcggccg tctcgtaggc ctcttccagc aggtatttca ccagcgtggc gtgggtctgc
     1921 tcggcatccc acggacaccc gcccggcgac cgcaaccggt ccatgacctc cacgagatcg
     1981 agaaaccgct ccccgcgccg tcccgccacg cttgccgtct tgccttgctc agccggtcgg
     2041 ggcgggcgag cccgaggcgg gcgaggtgaa cgcgtcactg gccaccacgt cgaactggac
     2101 ggactgccac gcgccgtagc gcggattcac gctgacgcct tcctgcttgg cgatcgtgcc
     2161 gaggtaggag gtcagcgccg ccgattgggc ggtctggtcg gtctggcccg gggcaagcgc
     2221 ggtgatcagc ttgttaagaa gcagcgcctg gtacaccgcc gtgtgcaggc cggcgaatgg
     2281 gatgccgttc gcctttgcgg tggcctcgtc gctgccgttg gtctggcgct cctgggcgag
     2341 cagcgactgg atctccgcct cggtcaccgt cacgcccttc gccttcgcgg cgtcggcgat
     2401 cagatcgtca cggatgatct gcgtcaagct cgacgtcact gcagtcgagc ggtcgggcag
     2461 ctggcccgtc gtggcatccg gtg
//
LOCUS       DATCBJ010000131         2483 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_391669, whole genome shotgun sequence.
ACCESSION   DATCBJ010000131 DATCBJ010000000
VERSION     DATCBJ010000131.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2483)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2483)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2483
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_391669"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2229)
                     /locus_tag="VII50_02665"
     CDS             complement(<1..2229)
                     /locus_tag="VII50_02665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5049776.1"
                     /translation="MADESAVIGDPQRFRLRRAGVLNVWQYDDQVFEFAGGRLLLRGA
                     NGAGKSKTLEMLLPFVLDGDKLHLTASGRHHTSLIWLMLDGADLEATTRTGYLWVEFT
                     RPADDVQGRSAEEVFTCGVGIRASQSSRSVTSWYFTYPGRVGDDLVLEDDLGPLGRER
                     CREALGSVGAFFDSARQYKEHVGRRLFGLDITRYNELLRLLYWLRQPQIGEDIEPGRL
                     ADILAIALPELDEVAVRSVGDSLDQLAEFGDELDRRARAADAVAGLAVTYRNYAAGVV
                     AERASALRTADRDRRHARRALEQRRRYLVGIDSALSDAEQHARNARREKTQADDAIRG
                     LEAGPEARSEQVLLEKDRRARELRQVANQALARASSDEQRLRERSAQDEQRTSALLGE
                     VGAIATQSHALATELAHCDVDTAFAPPAVFGQPRLREHGDAEAIVAAVDVLTTSLREA
                     TDALGARLAAVHVVRDALDDVRRADAARADAQRVEEAAEQREEAAARRHETANAAAGT
                     AEEQLRGDLRQWATLPLSAQVDVPAVALPDDFTNAALDELPNVARAAAQPGVETERAA
                     EIEAASRAASATAALAQLHSRRTAVEAQPEPAPAPPALPRLSRDETAVPLWRLVDFAA
                     DVPQPERAAIEAALESSGILDAAIRPDGSVLAPGEFDTVLSNGPATSAGPTLADVLVV
                     TVADTKVPADVVAGVLRRVVVRERLDDVEDPAHAAIGRDGGWCLGVLSGRASKPVPQY
                     VGA"
     gene            complement(2244..>2483)
                     /locus_tag="VII50_02670"
     CDS             complement(2244..>2483)
                     /locus_tag="VII50_02670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5049777.1"
                     /translation="SGALRRAYKDDMELLQHDALEVLVSFGLITRSADGCLVHAAAAR
                     YRVHTTLADDGPSHERSLFAEIDIDPANRSSDGEG"
BASE COUNT          420 a          846 c          800 g          417 t
ORIGIN      
        1 cgcaccgaca tactgcggca ccggtttcga ggcgcggccg ctgagcaccc cgaggcacca
       61 gccgccatct cgtccgatcg ccgcatgcgc ggggtcttcg acgtcgtcga gccgttcacg
      121 cacaacgacg cgccgcaaca cacccgcgac gacgtccgca ggaactttcg tgtcggcaac
      181 agtgacaacc aggacgtcgg ccagtgtcgg cccagcgctc gttgcgggtc cgttggacag
      241 cacggtgtcg aactcgccag gtgcgagaac cgacccgtca ggacgaattg ccgcgtcgag
      301 gatcccggac gattcgagcg cagcttcaat cgcggctcgt tccggttgcg gcacgtcggc
      361 ggcgaaatcg acgagtcgcc agagcggaac ggcagtctcg tcgcgactca gtcgcggcag
      421 cgcgggaggt gcgggcgcgg gctccggttg cgcctcgacc gcggtccggc gggagtggag
      481 ttgcgccagt gcggcggtcg cgctcgctgc tcggctggct gcctcaattt cggccgcgcg
      541 ctcggtctcg acccccggtt gcgcagccgc tcgggcaacg tttggcagct cgtcgagggc
      601 agcgttggtg aaatcgtcgg gcagggcgac agctggcaca tcgacctgcg cggaaagtgg
      661 cagcgtcgcc cattggcgaa ggtcgccacg aagctgttct tctgcagtgc ccgcggcagc
      721 attggcagtc tcgtgccgac gagctgcggc ttcctcgcgc tgctcggccg cctcctcgac
      781 tcgctgagcg tcagcgcggg cggcgtcagc cctcctgaca tcgtcgagtg cgtcacgaac
      841 gacgtggacg gcggccagcc gagcaccgag cgcatcggtc gcttctcgaa gcgacgtcgt
      901 cagtacgtca acagccgcta cgatcgcctc ggcatcaccg tgttcacgca aacgtggctg
      961 cccgaagacg gcgggaggcg cgaacgcggt atcgacgtcg cagtgggcga gctcagtagc
     1021 aagcgcgtgc gattgggtcg cgatcgcgcc tacttcgccg agcagggcgc tggtccgctg
     1081 ctcgtcttgt gcgcttcgct cgcgcagtcg ttgttcgtcg gagctcgcgc gagccagcgc
     1141 ttgattggcg acctggcgca gctcgcgtgc ccgccggtcc ttttccaaca acacctgctc
     1201 gctgcgcgcc tcgggacctg cctccagccc acgaatcgca tcatcggctt gtgtcttttc
     1261 gcggcgtgca ttgcgcgcgt gctgctcagc gtctgacaat gcggaatcga tgccgacgag
     1321 atagcgacga cgctgttcaa gcgcgcgccg agcatgccgg cgatctcgat cggcggtacg
     1381 caaggcactg gcgcgctcgg cgaccacccc agctgcataa ttgcgatagg tcaccgcgag
     1441 acccgcaacc gcatctgcag cccgtgcgcg acggtcgagt tcgtcaccga attccgcgag
     1501 ctggtcgaga gagtcaccaa ctgagcgcac tgcaacctcg tcgagctcag gcaacgcgat
     1561 ggcgaggatg tcggcgagcc gtcccggttc gatgtcctcg ccgatttgcg gttggcgtaa
     1621 ccagtacagc aaccgcagca gttcgttgta gcgcgtgata tcgagcccga agagccggcg
     1681 tccgacatgt tccttgtatt gccgcgcact gtcgaagaac gcgcccacgg aaccgagcgc
     1741 ctctcggcag cgttcgcgac cgagcgggcc aaggtcgtcc tcgagcacga ggtcgtcacc
     1801 aacccggccc ggataagtga agtaccacga cgtcacagag cggctggact gcgacgccct
     1861 aatccccacg ccacacgtaa agacctcctc cgcggatcgc ccttgcacgt cgtcagccgg
     1921 gcgggtgaac tcgacccaca agtagccggt gcgtgtcgtc gcctcaaggt cggcgccgtc
     1981 gagcatcagc cagatcaggc tggtgtggtg ccggccggac gccgtcagat gcaacttgtc
     2041 gccgtcaagg acgaacggca gcagcatctc cagcgtcttc gatttgcctg ctccattggc
     2101 tccacgcaac agcagccgcc cgcccgcaaa ctcgaatacc tgatcgtcgt actgccagac
     2161 gttgagcact cctgcgcgac gcagcctgaa tcgctgcggg tcgccgatca ccgcggactc
     2221 gtcggccatc agtcgcacga cgctcacccc tcgccgtcgg acgacctgtt ggccggatcg
     2281 atatctatct cggcaaatag agaacgctcg tggctcggtc cgtcgtccgc gagcgtcgta
     2341 tgcacacggt aacgcgcggc tgcggcgtgt acgagacagc cgtctgccga ccgcgtgatc
     2401 agcccgaacg acacaagcac ctcgagggca tcgtgctgaa ggagctccat gtcgtccttg
     2461 tatgcacggc gcagcgcacc aga
//
LOCUS       DATCBJ010000132         2872 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_207600, whole genome shotgun sequence.
ACCESSION   DATCBJ010000132 DATCBJ010000000
VERSION     DATCBJ010000132.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2872)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2872)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2872
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_207600"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1156)
                     /locus_tag="VII50_02675"
     CDS             complement(<1..1156)
                     /locus_tag="VII50_02675"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     substrate-binding protein"
                     /protein_id="HEY5049778.1"
                     /translation="MQKNVLTTVVAAGAIGAMVLAGCSSSKKTTAGGLGASASASAST
                     GGGSCNGTGTTYKIGYEGPLTGDNKQLGINMINAVNLAVDEANAAKNLCFQLAVLQSD
                     DGGTAAGAPAAAAALLQDSAVVGVIGPAFSGATAAVGAKYSAADMPLISPSATNATLT
                     SQGFTTFHRIVPTDGIEGKATADYLATKYKTAFVVDDTSTYGAGVAQVVAAELKAKGV
                     KVDTQSIAPTTDYSAIATKVASSGDATMYYGGYDAQAGLLAKALQAASYKGFEISGNG
                     GKSSVFTSTAGAAGDGYYFACGCLDATTAPSAAAFNTAYTAKFNTPPSTYSPEAYDAT
                     NAMISAISAAQKAGGVTRASVETAVNALDYTGITTTVKFTSTGEVAQATVN"
     gene            complement(1322..2086)
                     /locus_tag="VII50_02680"
     CDS             complement(1322..2086)
                     /locus_tag="VII50_02680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007588074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY5049779.1"
                     /translation="MREDHVGEKPTPTPADIAKGRQSPSRPETTGRTPGAADAQPPRP
                     ARSRVVIAEDEALIRLDLKETLDELGYEVVGEASDGEQAVALARELRPDLVILDVKMP
                     VLDGISAAQRIAAAGIAPVVILTAFSQRDLVERARDAGAMAYLVKPFSSNDLLPAIEM
                     ALSRYAEAAALGAEVSNLTEQLATRKVLDRAKGILQATRQLSEPEAFRWIQQSAMDQR
                     VSMRSVADAVIAAETASGSATTTSGDVGDTLPPIRS"
     gene            2154..2226
                     /locus_tag="VII50_02685"
     tRNA            2154..2226
                     /locus_tag="VII50_02685"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2187..2189,aa:Leu,seq:caa)
     gene            complement(2251..>2872)
                     /locus_tag="VII50_02690"
     CDS             complement(2251..>2872)
                     /locus_tag="VII50_02690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0030955 - potassium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HEY5049780.1"
                     /translation="ATQMLETMIGASRPTRAEVSDVANAVLDGADALMLSGETSVGAH
                     PSLAVETMARIIATVENGALETIPVVGGKARTKGGAIAHAAAEVGADLGARYLVAFTE
                     TGDSARRLARYRSAIPLLAFSPVGSTRNQLALTWGVETFLVAPVSHTDQMVRQVDRAL
                     LDLERCQLGELVVIVAGSPPGVPGSTNTLRVHRIGDTIESDAAAYS"
BASE COUNT          467 a          948 c          939 g          518 t
ORIGIN      
        1 ggttgactgt cgcctgcgcg acctcgccgg tcgacgtgaa cttcaccgtc gtggtgattc
       61 cggtgtagtc caacgcgttg actgcggtct ccaccgatgc cctggtgact ccgcccgcct
      121 tctgtgcagc ggagatagcc gagatcatcg cgttcgtcgc gtcgtaggcc tcaggtgagt
      181 acgtcgacgg cggggtgttg aacttcgccg tgtaggccgt gttgaacgct gcagcggacg
      241 gagccgtcgt cgcgtcaagg caaccacatg cgaagtagta cccgtcacca gctgcgccgg
      301 cagttgacgt gaagacgctg gactttccac cgttaccgga gatctcgaag cccttgtacg
      361 acgccgcctg caacgccttg gccagcaagc cagcctgtgc gtcgtaaccg ccgtagtaca
      421 tcgtcgcgtc gccagacgag gcgaccttgg tcgcgatcgc gctgtagtca gtggtcggcg
      481 cgatcgactg ggtgtcgacc ttgacgccct tggccttcag ctccgccgcg acaacctgag
      541 ccacgcccgc gccgtaggtc gacgtgtcgt cgacgacgaa agcggtcttg tacttggtcg
      601 ccaggtagtc ggcggtcgcc ttgccctcga tgccgtcggt cggcacgatg cggtggaacg
      661 tggtgaagcc ctgactggtc agcgtcgcgt tggtggccga ggggctgatc agcggcatgt
      721 cggcggcgga gtacttggca ccaacagcgg cggtcgctcc ggagaacgcc gggccgatga
      781 cgccgactac cgcgctgtcc tgcagaagcg cggcggctgc ggccggcgcg ccggcggcgg
      841 tgccaccgtc gtcggactgg agtaccgcga gctggaagca caggttcttc gccgcgttgg
      901 cctcgtcgac tgcaaggttg accgcgttga tcatgttgat accgagctgc ttgttgtcac
      961 ccgtgagcgg gccttcgtaa ccgatcttgt acgtggtgcc ggtcccgttg caggaaccgc
     1021 cgcccgtgga agcagacgcc gaagcggatg cgccgagacc gcctgcggtt gtcttcttgc
     1081 tgctcgagca gccggccagg accatcgcac cgatggcgcc agctgccaca actgtcgtaa
     1141 ggacattttt ctgcaccgag gtgcctccaa cgagttgagc gcgcggacat ttgccgtgcg
     1201 tactgatgac tgcgtgggac agtagccgac caggcggctg ttgataaatc ctgctgccta
     1261 taagttgcgg tcgtaaccaa atcttattcc aatggtgacc gcgatatcat gatttggtat
     1321 gttatgaccg tatcggcggg agcgtgtcac cgacatcccc ggaagtagtc gttgcacttc
     1381 ccgacgcagt ctccgccgcg atcacagcgt cagcaactga gcgcatcgag acacgttggt
     1441 ccatggcgga ctgctgaatc cagcgaaagg cttccggctc actcagctgg cgagttgcct
     1501 ggaggatgcc tttggcgcga tcgagcactt tgcgggttgc cagctgctcg gtcaggttcg
     1561 agacctcggc ccccagcgcc gccgcctcgg cataccggct aagggccatc tcgatcgccg
     1621 gcaacaagtc gttggagctg aacggcttga ccaggtacgc catggcgccg gcatcgcggg
     1681 cccgctccac caggtctcgc tgggagaacg ccgtcaggat gacgaccggc gcgatgcccg
     1741 ctgccgcgat tcgttgcgct gcactgatgc cgtccagtac gggcatcttg acgtcgagga
     1801 tgactaaatc gggccggagc tctcgggcaa gcgcgacagc ctgttcaccg tcgctggcct
     1861 caccgacgac ctcgtagccc aactcgtcga gcgtctcttt gaggtccagc cggatcagcg
     1921 cttcgtcttc ggcgatgacg accctcgacc gcgctgggcg cggcggctgt gcgtcggcag
     1981 cgcccggcgt ccgcccggtc gtttcggggc gggacggcga ttgacggccc ttcgcgatgt
     2041 cagccggcgt cggcgtgggc ttctccccga cgtggtcctc ccgcacacgc gcagcctacc
     2101 cgcagcgata gattggccgg cgcactgcaa gccttgccgg gaatccggcg gcagccccgg
     2161 tagcccaacg gcagaggcaa tggtctcaaa caccatccag tgtcggttcg aatccgaccc
     2221 ggggtaccga gcgcagcgag ccccagctac ctagctgtag gcggcggcgt cggattcgat
     2281 cgtgtcgccg atgcgatgca cgcgcagcgt gttggtcgag cccggcacac cgggtggact
     2341 gcccgcgacg atgaccacga gctcgccgag ctgacaccgc tcgaggtcga gcagggcccg
     2401 gtcgacctgg cgcaccattt ggtcggtgtg ggataccggc gccaccagga aggtctcgac
     2461 tccccaggtg agcgcgagct gatttcgggt ggaaccaacc gggctgaagg ccagcagcgg
     2521 aatcgccgac cggtaccgcg cgagccgccg cgcgctatcg ccggtctccg tgaacgcgac
     2581 caagtaccgc gcaccaaggt ccgcaccaac ctccgcggcc gcgtgcgcga tcgcgccgcc
     2641 cttcgtccgc gccttcccgc cgacaaccgg gatcgtttcg agagcgccgt tctcgacggt
     2701 ggcgataatg cgcgccatcg tctccaccgc cagcgacgga tgggcgccga cactcgtctc
     2761 acccgacagc atcagcgcat cggctccgtc gagcacggcg ttggcaacat cggaaacctc
     2821 ggctctggta ggccgcgatg ccccgatcat cgtctcgagc atctgagtgg cc
//
LOCUS       DATCBJ010000133         2243 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_96816, whole genome shotgun sequence.
ACCESSION   DATCBJ010000133 DATCBJ010000000
VERSION     DATCBJ010000133.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2243)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2243)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2243
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_96816"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(313..1695)
                     /locus_tag="VII50_02695"
     CDS             complement(313..1695)
                     /locus_tag="VII50_02695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5049781.1"
                     /translation="MLNRARLLALAMLLPFVHATTACTDGSDLPPALDAGSADTPPGS
                     SGGNCTSVAAQSSDAGFSLPVAPCVDESSLGPGQLLLTASGESLALQGYTFPDPNGTF
                     ADGWAVKFTHYIATFDKVSLWSDPDSVPTDQSRVGPLVAELDGPWAVDMHLDGPAFPY
                     VDGKEAGERAIAFAVLTSENRNGGGALPTDGTRLAVGFSAVQPTPSALNVNLDADGLD
                     AYGYMIQNHCTVYYRGTATWVGDTKGGLCGPDAGNGKGGEAEFAKIPTTVDFEFCFAP
                     ASVGSLQPGAPETSYVNCDNQDNDPAMPLNGEPHQRGVAFPSNKFAVGEVTFHTDHPF
                     WESTEHDTPARFDQFAARAVGSSIDGGVPLVHFSDVIGVNYLAFTDREGSVLPWRTCD
                     PSYQSPNGGSRLGQMSFDPVKVPHCTGEDHATGLCDYYDFSKYDQSTQGHWNGADGLC
                     FVQRHYPSPQ"
     gene            complement(1721..>2243)
                     /locus_tag="VII50_02700"
     CDS             complement(1721..>2243)
                     /locus_tag="VII50_02700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5049782.1"
                     /translation="SLQEIHVTGEGSTIPAAFAQVWLTHASAISGGDLNEPDDAPILT
                     LQGSYEDATGTVVTFSGAISIDTTRIQTMNSALPGEIQICQQRIVSGIPVDLTIAQSG
                     TLLLQLDPATLFNGVSFSDLPDAGAVASGCADGTTASRCFTNDSSNTSSRTLFANLTS
                     VGPYRFAWLSSAP"
BASE COUNT          350 a          724 c          778 g          391 t
ORIGIN      
        1 ctttccgccc ggcggtgggt tgagcgaccc acccgagttc gagctgcatc cgctcgcgat
       61 cgcggacgcg acgacgactg cgccaaatcc caagcctgca accagccaaa cggaatgtcg
      121 catgggggct cctcgtttgc gcacgaacgt acgactcgcg tacgggcgta acgagataga
      181 gaatgatctg gaattgaagc cctggctagc ccactcgggg tgggaatcgg tgatccagat
      241 ccgcgctgga tccaggtgtt tcggcgcacc gggacatcgg ttcggtgctt cgcttgcgct
      301 tctatcgcgc tcttattgcg gcgacgggta gtgcctctgc acgaagcaga gcccatcggc
      361 accgttccag tggccctgcg tgctctggtc gtacttcgag aaatcgtagt agtcgcaaag
      421 ccctgtcgca tgatcctcgc cggtgcagtg aggcaccttg accgggtcga agctcatctg
      481 accgaggcgg ctgccgccgt tggggctctg gtagctcggg tcgcacgtac gccacggcag
      541 gacgctgccc tccctgtccg tgaacgcgag atagttgacg ccgatgacgt cggagaagtg
      601 aacgagcggc acgcccccat cgatcgagct gcccacggcc cgcgcggcga actgatcgaa
      661 gcgcgccggg gtgtcgtgct cggtgctctc ccagaaggga tgatcggtgt ggaacgtcac
      721 ctccccgaca gcgaacttgt tgctcgggaa ggcgacccca cgctgatgag gctcgccgtt
      781 gaggggcatc gcagggtcgt tgtcctggtt gtcgcagttg acatagctcg tctcgggcgc
      841 gccaggctgg aggctgccga cgctcgcagg cgcgaagcag aactcgaaat cgaccgttgt
      901 cgggatcttc gcgaactcgg cctcgccgcc cttgccgttt cctgcgtccg gcccgcagag
      961 gccgcccttg gtgtcgccca cccaggtcgc cgtgccgcgg tagtagacgg tgcagtggtt
     1021 ctggatcatg tagccatagg catccaggcc gtccgcgtcg aggttcacgt tgagcgcgct
     1081 gggtgtcggc tggaccgcgc tgaaaccgac ggccaggcgc gtgccgtccg tcgggagcgc
     1141 gccgcccccg ttcctgtttt cgctcgtgag gaccgcgaat gcgatcgcgc gctcgcccgc
     1201 ctcctttccg tcgacgtagg ggaacgcggg gccgtcgagg tgcatgtcga cggcccacgg
     1261 cccatcgagc tcggccacga gagggcctac ccgggactgg tcggtgggca cgctgtccgg
     1321 atcgctccag agcgagacct tgtcgaacgt cgcgatgtag tgcgtgaact tgacggccca
     1381 gccgtccgcg aaggtgccgt tcggatcggg gaaggtgtag ccttgcagcg cgaggctctc
     1441 accggatgcg gtcaggagga gctgacccgg tcccagcgac gactcgtcaa cgcacggggc
     1501 gacgggcaaa gagaagcccg cgtccgagct ctgcgcggcg acgctcgtgc agttgccgcc
     1561 cgacgagccc ggaggcgtgt ccgcgctccc cgcgtcgagc gcgggcggca gatcgctgcc
     1621 gtcggtgcac gccgtcgtcg cgtggacgaa cgggaggagc atcgccaggg cgagaagcct
     1681 tgctcggttc agcatgctgg gagcctcctc gctcgctcga tcacggggcc gacgacagcc
     1741 acgcaaaccg gtacgggccc acggatgtca ggttggcgaa cagcgtccgg ctcgacgtgt
     1801 tgctgctgtc gttggtgaag caccggctgg ccgttgtgcc gtcggcgcag cccgacgcga
     1861 cggcccccgc gtccggcagg tcggagaagg agacgccgtt gaagagcgtc gccgggtcga
     1921 gctgcaagag cagcgtcccg gactgcgcga tggtgagatc cacggggatc ccggacacga
     1981 tgcgctgctg gcatatctgg atctcgcctg gcagggcgct gttcatcgtc tggatccgcg
     2041 tcgtgtcgat gctgatcgcg cccgagaagg tgactaccgt tcccgtcgcg tcctcgtagc
     2101 tgccctgcag cgtcaggatg ggggcgtcgt cgggctcgtt cagatcgccg ccggagatag
     2161 cgctcgcgtg ggtcagccac acctgcgcaa acgccgcggg gatcgtggat ccctcgcccg
     2221 tcacgtgaat ctcttgcagc gac
//
LOCUS       DATCBJ010000134         4416 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_22786, whole genome shotgun sequence.
ACCESSION   DATCBJ010000134 DATCBJ010000000
VERSION     DATCBJ010000134.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 4416)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4416)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4416
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_22786"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..649
                     /locus_tag="VII50_02705"
     CDS             <1..649
                     /locus_tag="VII50_02705"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HEY5049783.1"
                     /translation="DSLVEIPDGVALREAAAVGIVGLTAFRIVEIAEIGSADRVLVLA
                     GSGSVGLAAISYAASKGAQVWGQTGNEAKADAIVAMGATQAVVCDATGLTDAVQAFEP
                     TAVLDSLGGPFTPAALAALVPRGRLLIFGASAGAEGTISFLQLYRKQQRLLTYGGLGA
                     TVPERREGIAGALAAVAGGKMRIHIGAELGLDEVGEAFALVAGRKVTGKVILTLR"
     gene            709..1569
                     /locus_tag="VII50_02710"
     CDS             709..1569
                     /locus_tag="VII50_02710"
                     /inference="COORDINATES: protein motif:HMM:NF025736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEY5049784.1"
                     /translation="MPNVALSWQAAALIGAACFAVATMVERFAPDRRSVIFRIAPFGR
                     EVGIILGLYALWQIAGGVSLGHTDEAVKRGQWVWDHERAWHFPSEVALQRPILGHPLL
                     VQVCNIYYATMHFGMLLAVLLWLYIRHRDAYRRTRMLIVLTTTACLLVGLVPVAPPRL
                     IHVGMVDTAAAYGQSVYAVTGQLGADQYSAMPSVHVAWAFLVAFAVVGATRSRWRWLI
                     LAHTAATVYVVVVTANHFWLDGVVAVALLGISLALQMVSRRIVLRHRRLTRRPIDERP
                     DAELLRVDAN"
     gene            complement(1580..2140)
                     /locus_tag="VII50_02715"
     CDS             complement(1580..2140)
                     /locus_tag="VII50_02715"
                     /inference="COORDINATES: protein motif:HMM:NF024929.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HEY5049785.1"
                     /translation="MASAATVTAAQLGKSWHVGCPVGPAQLRSLTMTYWGFDAKPHMG
                     TLVVNASVVPAVTQVFRTLFGDHFPIRTMIPIAAFGASDPASTAADNTAGFNCRYAVA
                     NGPPQWSAHAYGEAIDVNTVENPYVFNGQVLPSPQAAAYKERTPYRPGMAVPGGQLVN
                     AFAAVGWLWGGRWTAPDYQHFSSTGG"
     gene            2172..2555
                     /locus_tag="VII50_02720"
     CDS             2172..2555
                     /locus_tag="VII50_02720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5049786.1"
                     /translation="MGDGWLVGDGLLVGRGVGRTDGLGVGEWLGAGEWLGLGLGVGLA
                     VGVWLGLAAVVRGEDAIAGASDDSGAAGLVTSDLLAGETELGALPSRWLLVQPAAVRP
                     MITLTASIAVRPAGVSCARLPTGSG"
     gene            2602..3315
                     /locus_tag="VII50_02725"
     CDS             2602..3315
                     /locus_tag="VII50_02725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HEY5049787.1"
                     /translation="MNHEHDLIDTIEMYLRTIFELEEEGAVPLRARIAERLLQSVPTV
                     SQTVARMERDGLVTIDGDRRLELTPAGRAIATRVMRKHRLAECLLVDVIGLEWELVHE
                     EACRWEHVMSEAVERRLLDVLGHPQESPYGNPIPGLAELGEGTDDVEFREGLVALSAV
                     PESTHNVIVRRIGEPLQSDAALLVRLRRAGIRPNMPVRISRVGGELLVGREGDAVAFG
                     DAVTSHVFVSTAPSVATAS"
     gene            complement(3331..3852)
                     /locus_tag="VII50_02730"
     CDS             complement(3331..3852)
                     /locus_tag="VII50_02730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5049788.1"
                     /translation="MTSSAAGGPILGRYTHDISDETDLRTVRLLGMPIALFLRAREHH
                     DELIREFTLMAIRTNTQRGPALPPRLRELVDILGRRFGASTSRADMERDAAIERGDAT
                     VDLTYHVPVSLAADLQMLTQLMDDADDFCRMEKLLTLPRDPSMVAFSHWYNGEFLRQI
                     DGLPPIPWNGPLE"
     gene            complement(4011..4181)
                     /locus_tag="VII50_02735"
     CDS             complement(4011..4181)
                     /locus_tag="VII50_02735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009079878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HEY5049789.1"
                     /translation="MGTTDKVKNTAQSAKGSVKEKAGQVTGNRDMEAEGKLDKAAGSL
                     KQAGEKVKDAFK"
BASE COUNT          661 a         1424 c         1500 g          831 t
ORIGIN      
        1 cgactccctc gtggagatac ccgacggtgt cgcgctccgc gaggcggcgg cggtcggcat
       61 cgttgggctc acagcgtttc gcatcgtcga gatcgccgag atcggatcgg ccgatcgggt
      121 tctcgttttg gccggcagcg gctctgtcgg gcttgccgcg atttcttacg cagcgtccaa
      181 aggcgcgcag gtatgggggc agaccggcaa cgaggcgaag gccgacgcga tcgtcgcgat
      241 gggcgcgacg caggcggtgg tgtgcgacgc caccgggttg accgatgccg tccaggcctt
      301 cgagccaacc gcggtgctcg actcgctcgg cggaccgttc acgcccgctg ccttggccgc
      361 gctcgttccg cgcggccgct tgctgatctt cggcgccagc gcaggcgccg aaggcaccat
      421 ttcgttcctg cagctgtacc gcaaacagca gcggttgctc acctacggcg ggcttggcgc
      481 gaccgtgccc gagcggcgcg aaggcattgc cggtgccctc gcggcggtcg ccggcggcaa
      541 gatgcggatc cacatcggtg ccgagcttgg cctcgacgaa gtcggcgagg cgttcgccct
      601 cgtggccggc cgcaaggtga ccggcaaggt gatcttgacg ttgcggtgat cttgacgttg
      661 cggcgacctg cgagcacgcg acgttgaggg ggtgcgcgat gatgtcccgt gcctaatgtc
      721 gcgctgagtt ggcaggccgc cgcgctgatc ggcgccgcgt gcttcgcggt cgcgaccatg
      781 gtggagcgct tcgcgcctga ccgtcgctcc gtgattttta ggatcgcgcc gtttggacgc
      841 gaggtcggca tcatccttgg cctctacgcg ctctggcaga tcgccggcgg cgtatcgctc
      901 gggcacaccg acgaggccgt gaagcgcggc cagtgggtgt gggaccacga gcgcgcgtgg
      961 cactttccga gcgaggtcgc gctgcagcgg ccgatactcg ggcacccgct gctcgtgcag
     1021 gtgtgcaaca tctactacgc cacgatgcac ttcgggatgc tgctcgccgt cttgctgtgg
     1081 ctctacatcc gccatcgcga cgcgtatcgc cgcacccgca tgttgatcgt gttgaccacg
     1141 acggcgtgcc tgctagtcgg cctggttccg gtcgcgccgc cgcgtcttat ccacgtgggc
     1201 atggtcgata ctgcggccgc atacgggcag tcggtgtacg cagtgaccgg ccagcttggc
     1261 gccgatcagt attcggcaat gccgtccgtg cacgtggcct gggcgttctt ggtcgcgttt
     1321 gctgtggtcg gtgcgacgag aagccggtgg cgatggctca ttctggccca caccgccgcc
     1381 accgtttatg tcgtcgtcgt aaccgccaac cacttctggc tcgacggggt cgtcgcggtc
     1441 gcgctactag gaatttccct tgccctacag atggtttcgc gacggatagt gttgcggcat
     1501 cgccgactca cgcgacgtcc gatcgatgaa cgcccggacg ccgaattgct gcgcgtcgac
     1561 gcgaactgac cgtcgttcgt cagccgccgg tcgacgagaa gtgctgatag tccggtgccg
     1621 tccagcggcc gccccagagc caccccactg ctgcgaacgc gttgacgagt tgtccgccag
     1681 gcacggccat gccagggcga taaggcgtcc gctccttata ggccgcagcc tgcggcgacg
     1741 gcaacacttg accgttgaac acgtacggat tctcgacggt gttcacgtcg atggcttcgc
     1801 catatgcatg cgccgaccat tgcggcggtc cattcgcgac cgcgtacctg cagttgaaac
     1861 cagcagtgtt gtctgccgct gtcgatgccg ggtcactcgc gccgaacgcg gcgatcggga
     1921 tcatcgtgcg gatcgggaag tggtcgccga acaaggtgcg gaacacctgc gtgaccgcgg
     1981 gcacgactga tgcgttgacg accagcgtgc ccatgtgcgg tttcgcgtcg aacccccaat
     2041 acgtcatggt gaggctgcgc agttgcgcag gaccgaccgg acagcccacg tgccagctct
     2101 tgccaagctg cgcggccgtc acggtcgcgg cacttgccac gaaaacagcc gggacgacgg
     2161 cggcttggct ggtgggagac ggctggctgg tcggcgacgg tttgcttgtg ggacgaggcg
     2221 tcggccgcac ggacgggctc ggcgtcgggg aatggctcgg cgcaggggaa tggctcggac
     2281 tcgggctcgg agttggtctc gcagtcggcg tctggcttgg gctcgcggcc gttgtgagag
     2341 gggaagacgc gatcgccggc gctagcgatg acagcggtgc cgcggggctc gtgacctcag
     2401 atctgctggc gggggagacc gagctgggtg cgctgccatc tcggtggctg ctcgtgcagc
     2461 cggcggctgt gaggccaatg atcacgctga ccgcgagcat cgccgtccgc cctgctgggg
     2521 tttcgtgcgc gaggttgccc accggctcag gctaaccggc ggtctacgca ccggtcggca
     2581 aagtgcgaca caatcgatgg cgtgaaccac gagcacgatc tgatcgacac gatcgagatg
     2641 tacctgcgga ccatcttcga gctcgaggaa gagggcgcgg tgccgttgcg tgcacggatc
     2701 gccgagcgat tgttgcagag cgtcccgacg gtgtcccaga cggtggcccg gatggagcgt
     2761 gacggcctcg tcaccatcga cggcgaccgg cgtctcgagc tcactcctgc gggtcgggcc
     2821 atagccacca gggtcatgcg caagcaccgc ctcgccgagt gcttgctggt cgatgtgatc
     2881 ggcctcgaat gggaactcgt gcatgaagag gcatgccgct gggagcacgt catgagtgag
     2941 gccgtcgagc ggcggctgct tgacgtgctc ggtcatccgc aggagtcccc gtacggcaac
     3001 ccgattccgg gcctggctga gctcggcgaa ggcaccgatg acgtcgagtt ccgcgagggc
     3061 ctagtggcat tgagcgccgt ccccgaaagt acccacaacg tcatcgtgcg gcgcatcggt
     3121 gaaccacttc agtccgacgc cgcactgctg gttcggctac gtcgtgcggg catccgcccc
     3181 aacatgccgg tgcgcatctc acgcgtcggc ggtgagttgc tcgtcggtcg cgagggcgac
     3241 gcggtcgcct tcggggacgc ggtcacgtca cacgtgtttg tctctaccgc gccgtcagtc
     3301 gcgaccgcgt cctgagcgtt tctgaacctt ctactccagc ggcccgttcc atgggatcgg
     3361 cggcaggccg tcgatctggc gcaggaactc accgttgtac cagtgggaga acgccaccat
     3421 gctcggatcg cgcggcaacg tcagcagctt ctccatacga cagaagtcat ccgcgtcgtc
     3481 catgagttgg gtcagcatct ggaggtcggc ggcgagactc accggtacgt gataggtcaa
     3541 gtcgactgtt gcatcgccgc gctcgatcgc ggcgtctcgc tccatgtcgg cccgcgacgt
     3601 gcttgcaccg aaccggcggc cgagaatgtc gacgagctcg cgaagccggg gtggcagcgc
     3661 agggcctcgc tgcgtgttcg tgcgaatcgc catcaaggtg aactccctga tgagctcgtc
     3721 atgatgttct cgggctcgca aaaacaacgc aatcggcatg ccgagcaatc ggactgtccg
     3781 cagatccgtc tcgtccgaaa tgtcatgcgt gtatcggccg aggattggcc caccggctgc
     3841 tgacgaagtc acgggcgaac ggtcatgggc gaacggtcat ggaaggtcgg tcatttgcgt
     3901 gccacttgca tggatcccat ttgaccaccg tctcagccgg tcgcggcaga ttggagcacg
     3961 acatgcgggg agggccagat gggctggccc tccccgcatg tcgggttcgg ttacttgaag
     4021 gcgtccttca ccttttcgcc cgcttgcttc aatgagccgg cggccttgtc cagcttgcct
     4081 tcggcttcca tgtcacggtt gcccgtcacc tgaccggcct tttccttgac gctgcccttc
     4141 gcggactgcg cggtgttctt caccttgtcg gtggtaccca tcggtcgctc ccctcgtggg
     4201 gtcctctttg caggtccctc tatctcggtc cccacctcgt acccacgagg tggcgcttta
     4261 tgccgaacag gcccccccga cggaaatcgc ctgcgacacg atcttggacg gttcggctcg
     4321 cgtcccccca ccatcgccgg gctcgcggca cgatcggcgg agcggccgct ccgctgccct
     4381 gcggcggccg tctccaggcg ccgtcaaagg gcggtg
//
LOCUS       DATCBJ010000135         2866 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_243833, whole genome shotgun sequence.
ACCESSION   DATCBJ010000135 DATCBJ010000000
VERSION     DATCBJ010000135.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2866)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2866)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2866
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_243833"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..391)
                     /locus_tag="VII50_02740"
     CDS             complement(<1..391)
                     /locus_tag="VII50_02740"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY5049790.1"
                     /translation="MTAGSGAPRGADSRTLAALGLGGFVVLGLPDGMWGTAWPTMRHT
                     FHQPLGSLGLILVFGTLGNLVTSLSTGRTLRRFGVGRLLVVAGLIAATGALLIAISSV
                     WAMVLVGAFLIGMAAGLLDTGLNTVVAL"
     gene            complement(388..1353)
                     /locus_tag="VII50_02745"
     CDS             complement(388..1353)
                     /locus_tag="VII50_02745"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEY5049791.1"
                     /translation="MTMFSKDFTIDPRRLRVLREVGLRGTVTSAASGLHLTPSAVSQQ
                     IAALSRDLGVPLLEKTGRGVKLTGQAQVILTHATVVQAQFERARADLAAFADGRTGQV
                     SVAGFATGIAGLIAPAMRQLRTTRPGITMTVIEMEPPDVFTRLDRGELDVVVAVDFRK
                     APPRNDPHYHRVELMSDVLDAMLPADHPLAHQTSIDLVDLASDVFVTASPGTSCSEVT
                     LATCAAAGFSPDVRNYSTEWGAVAALISVGAGVGLLPRLAQPLHCPGVVVRPLRGPKA
                     ARNIFAAVRAGAQDDPVLAATLEALCDVAAVVSSSPAATSPSSTA"
     gene            1403..1579
                     /locus_tag="VII50_02750"
     CDS             1403..1579
                     /locus_tag="VII50_02750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5049792.1"
                     /translation="MFTPYCDKHESTVLLGYESVVGIESTVFGVKVLLRCYCGELLVH
                     DAVNRNHAAAQMSA"
     gene            1698..>2866
                     /locus_tag="VII50_02755"
     CDS             1698..>2866
                     /locus_tag="VII50_02755"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY5049793.1"
                     /translation="MRVLPLIDPGVPDVRSPMRFLLWVVKGQRATLAGGMIFGIIWMC
                     GQATVPFVVGRGIDEGITGRSTGALVTWSAALLGLGVMQAAAGVSRHRFAVANWLTGT
                     FRVEQLLTRQAARLGASLTQRVQLGDIVSSATTDAGYIGNCLDITARLAGSIVSFVAV
                     AIIMLSTSVPLGLVVLIGVPVLMLLIGPLLRPLHHRQTELRASVGQLTNFGADTVAGL
                     RVLRGVGGEREFFTRYQARSQDVRRKGVSVAAVGSVLDAAEVLLPGVFVVLVTWLGAR
                     LAIQHKLTVGQLVSFYGYAAFLLAPMRIATEVADKFTRGLVAAKRVLRVLSLQPVLAQ
                     PLHPDVVPSNGDLTDTTSGLTVRSGEFVVVAAAEPSQASALADRLGRYVDPDGGER"
BASE COUNT          476 a          970 c          950 g          470 t
ORIGIN      
        1 acaaggcgac gaccgtgttg aggccggtgt cgagcaggcc tgccgccatg ccgatgagga
       61 atgcaccgac gagcaccatc gcccataccg acgagatcgc aatcagcaag gcgccggtgg
      121 ccgcgatgag tccggcaacc acgagcaacc gcccgacacc gaaacggcgc agggtacggc
      181 cggtcgacaa cgacgtgacg agattgccca atgtcccgaa caccaggatc agaccgaggc
      241 tgccgagcgg ttgatgaaac gtgtgccgca tcgtcggcca cgcggtcccc cacatgccgt
      301 cgggcagccc gaggacgacg aaaccgccca gccccaaggc agccagggtt cgtgagtccg
      361 cgccgcgtgg tgcaccggat cccgccgtca tgcagtggac gacgggctgg tggcggccgg
      421 tgagctgctg accacagcag cgacgtcgca caaagcctcg agcgtcgcgg ccagcaccgg
      481 atcgtcctga gcgccggccc gcacagccgc gaagatgttg cgggccgcct tcggcccgcg
      541 caatgggcgc acgaccacac caggacaatg cagcggttgc gccagtcgag gcagtagccc
      601 gacaccggca ccgaccgaga tcagtgccgc cacagctccc cactcggtgc tgtagttccg
      661 gacgtcgggc gaaaagccag ccgcggcgca ggttgccagc gtcacctcag aacacgacgt
      721 gccggggctg gccgtcacga agacgtcgct cgcgaggtcg acgaggtcga tagacgtctg
      781 gtgcgcgagc ggatggtcgg cgggcagcat cgcgtcgagc acgtcggaca tgagctcgac
      841 ccggtggtaa tgcggatcgt tgcgcggcgg cgccttgcgg aagtcgaccg caaccacgac
      901 atcaagctcg ccccgatcga gtcgggtgaa cacgtccggc ggctccatct cgatcacggt
      961 catcgtgatg cctggccgcg tcgtacgcag ttgtcgcatc gcaggcgcga tcagcccggc
     1021 gatgccggta gcgaatccgg cgacggagac ctgcccagtc cgtccatcag cgaacgcggc
     1081 gaggtctgcc ctggcgcgct caaattgtgc ctgcacgacg gtggcgtggg tcaagatcac
     1141 ctgcgcctgg ccggtgagct tgaccccgcg cccggtcttc tccagcagcg ggaccccgag
     1201 atctcgcgac agcgcggcga tctgctgaga gacggcggac ggcgtgaggt gcaggccgct
     1261 ggctgcgctg gtgaccgtcc cccgaaggcc gacctcccgc agcacgcgga ggcggcgcgg
     1321 gtcgatcgtg aagtctttac tgaacatagt catgtgcgaa tactagatgg acctgcacgg
     1381 ttttgtcagc caagatggat gcatgttcac cccctactgc gacaagcatg agtccaccgt
     1441 cctgctcggc tacgagtccg tcgtcggcat cgagtcgacc gtcttcgggg tcaaggtcct
     1501 gctccgctgc tactgcggcg aactgctcgt ccacgacgcg gtcaaccgca atcacgccgc
     1561 ggcacagatg tcggcctagg cctccactca ggtctagacc aacgcgagcc taaacctggc
     1621 tccaggacgc tccgaagcgc ttgcattcgc cgaaaccagc gtgcccccaa ggtcaccggc
     1681 agttaacgtg agtccacgtg cgcgtcctcc cgctgattga tcccggcgtc cccgatgttc
     1741 gctcgcccat gcgcttcctg ctctgggtcg tgaagggcca gcgggcgacg ctggcgggcg
     1801 gaatgatctt cggcatcatc tggatgtgcg gccaggcgac cgtgccgttc gtggtgggtc
     1861 gcgggatcga cgaaggcatc accggccgca gtacaggagc gctggtgacc tggtcggcag
     1921 ccctgctagg cctgggtgtc atgcaggccg cagccggtgt cagccggcac cgcttcgcgg
     1981 tcgcgaactg gctgaccggc accttccggg tcgagcaact gctgacccgg caggccgcgc
     2041 ggctcggtgc ctcgttgacg cagcgggtcc aactcggcga catcgttagc agcgcgacaa
     2101 cggacgccgg ttacataggc aactgcctcg acattacggc ccgactcgct ggctcgatcg
     2161 tgtcgttcgt cgccgtcgcc atcatcatgc tcagtacctc ggtaccgctc ggacttgtgg
     2221 tgctgatcgg tgtcccggtt ctgatgcttc tgatcgggcc tttgctacga ccgttgcacc
     2281 accggcagac cgagctgcgc gcatcggtcg gacagctgac caacttcggt gccgacaccg
     2341 tcgctggcct gcgtgtgctg cgcggtgttg gcggtgagcg ggaattcttc acccgctacc
     2401 aggcgcgatc ccaagacgtc aggcgcaagg gcgtttcggt ggcagccgtg ggctccgtgc
     2461 tcgacgctgc cgaggtgttg ctgcccgggg ttttcgtcgt cctcgtcacc tggctcggcg
     2521 cccgtctcgc gatccagcac aagctcaccg tcggccagct ggtgtcgttc tacggatacg
     2581 ccgcgttcct gctcgcaccg atgcgcatcg ctaccgaagt cgccgacaag ttcacccgcg
     2641 ggctggtggc ggctaagcgg gtgctgcgcg tgctctcgct ccagcccgtg ctggcgcaac
     2701 cgttgcaccc ggatgtggtt ccaagcaacg gcgatctcac cgacacgacg tccgggctca
     2761 ccgtgcgatc cggcgagttc gttgtcgtcg cggcggccga gccatcgcag gcgagcgcgc
     2821 ttgccgaccg gctcggtcga tacgtcgatc cggacggcgg cgagcg
//
LOCUS       DATCBJ010000136         3450 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_243813, whole genome shotgun sequence.
ACCESSION   DATCBJ010000136 DATCBJ010000000
VERSION     DATCBJ010000136.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 3450)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3450)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3450
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_243813"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(168..1388)
                     /locus_tag="VII50_02760"
     CDS             complement(168..1388)
                     /locus_tag="VII50_02760"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005466189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HEY5049794.1"
                     /translation="MPEAVIVATARSPIGRAHKGSMMQLRPDDLTATIVRAALDKVPQ
                     LNPGDIDDLMLGCGLPGGEQGFNMARVVAVRLGFDRLPATTVNRYCASSLQTTRMAFH
                     AIKAGEGDVFISAGVECVSRFVHGSSDNHPDTRNPYFAGAGARTASRAEGGTTSWEDP
                     RKEHRVPDIYIAMGQTAENVAQLRGISRAMQDEFGCRSQNLAEKALNAGFWQREITPV
                     TLPDGTVVDRDDGPRAGTTLEKLATLEPVFRPDGTVTAGNCCPLNDGAAAVIVMSDVK
                     ARELGITPLARIVATGVSALSPEVMGLGPVEASKQALRRANMSIKNVDLVEINEAFAA
                     QVIPSYQDLGIDLDKLNVNGGSIAVGHPFGMTGARITGTLLNSLKFHDAAIGLETMCV
                     GGGQGMAMLFERLS"
     gene            1519..2883
                     /locus_tag="VII50_02765"
     CDS             1519..2883
                     /locus_tag="VII50_02765"
                     /EC_number="4.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013229841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004122 - cystathionine beta-synthase
                     activity [Evidence IEA];
                     GO_process: GO:0019344 - cysteine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-synthase"
                     /protein_id="HEY5049795.1"
                     /translation="MDVHDSLLDLVGDTPLVRLSRIAAPGGAQLLAKLEQLNPGGSVK
                     DRIAVRMVETAEASGALKPGGTIVEPTSGNTGVGLAIVAARKGYRCVFVMPDKMSAEK
                     INVLRGYGAQVVVCPTAVAPEDPESYYSVSDRLVREIEGAWKPDQYSNPDNPRSHYET
                     TGPELWRQTDGQLTCFVAGIGTGGTISGTGRYLKEVSGGAVRIVGADPEGSVYSGGTG
                     RPYLVEGVGEDFWPTTYDRTICDDIIAVSDKDSFLMTRRLARDEGILVGGSSGMAVVA
                     AASVAASLSPSDVVVVLLPDSGRGYLSKIFNDEWMADYGFLAGSSSTSVADVLKRKGN
                     RLPEMVHLHPTETVREAIDVLREYGVSQVPVVRAEPPVMSAEVIGSVVERDLLDALFS
                     GRAALSDALEKHISAPLPMIGGGEPVAAAMTALETADAAVVLIDGKPAGILTRQDLLG
                     FLAH"
     gene            complement(2911..>3450)
                     /locus_tag="VII50_02770"
     CDS             complement(2911..>3450)
                     /locus_tag="VII50_02770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5049796.1"
                     /translation="VTFVRCVVGIVPFDTADFDWFAGMDPENVKEFGWALAGENTLYR
                     ELSKEADAMVARVTADAANVMSESWDLPDADREVFAHPLVMRVMREAVPECVVNGPWG
                     WVDDDLAFTRPWGFDVSEIAIPAEVHYGSQDVLVPAAHGEWLAANVPEALVVVKDDEG
                     HAASPERMLELLKSMIGPH"
BASE COUNT          547 a         1082 c         1163 g          658 t
ORIGIN      
        1 catcgttcga cccggttcgg gtcggccagc gcgagtgcaa cgcctgggcc gcctacgcga
       61 cgatccgttg ctggcgcggg agcctcgcgc gttggtcgcg tcatataccg cgctgcgcga
      121 gtgcgtcgag cgtcaccagc cggacggggg ggcaagggta gcgcgagtca gctgagccgt
      181 tcgaagagca tggccatgcc ttgaccgccg ccgacgcaca tcgtctcaag cccgatcgcc
      241 gcgtcgtgga acttcagtga gttcagcaag gtgccggtga tgcgggcgcc ggtcatgccg
      301 aacgggtgcc cgaccgcgat cgagccgccg ttgacgttga gcttgtcgag gtcgatcccg
      361 aggtcctggt agctcgggat cacctgggcg gcgaacgctt cgttgatctc gacgaggtcg
      421 acgttcttga tgctcatgtt cgcgcgccgc agtgcctgtt tgctggcctc gaccgggccg
      481 agtcccatga cctccggcga cagcgccgat acgccggtgg ccacgatgcg tgccagtggt
      541 gtgatgccga gctcacgcgc cttgacgtcg gacatcacga tcaccgcggc agcgccatcg
      601 ttcaacgggc aacagttgcc ggctgtgacc gttccgtccg gccggaacac cggttcgagc
      661 gttgccaact tctcgagggt cgtaccggct cgcggtccgt cgtcgcggtc aacgactgtt
      721 ccgtccggga gagtgacagg cgtgatctcg cgttgccaaa agcctgcgtt taaggccttt
      781 tcggcaagat tctgcgagcg gcagccgaac tcgtcctgca tcgcgcggga aatgccgcgc
      841 agctgcgcca cattctcggc ggtctggccc atcgcgatgt agatgtcggg aacgcgatgt
      901 tccttacgcg gatcttccca gctcgtcgtg ccaccctcgg ccctgctcgc ggttcttgca
      961 ccggcgcctg cgaagtaagg gttgcgggta tccggatggt tgtccgaact gccgtgcacg
     1021 aagcggctta cgcattcgac gccggcgctg atgaacacgt cgccctcacc ggctttgatc
     1081 gcgtgaaagg ccatccgggt tgtctgtaga gacgacgcgc agtagcgatt gacggtcgtc
     1141 gccggcagcc gatcaaatcc aagacggacg gcgacgactc gcgccatgtt gaatccctgc
     1201 tcaccgccgg gcagtccgca gccgagcatc aggtcgtcga tatcgccggg attgagttgc
     1261 ggcaccttgt cgagagcggc acgcacgatg gtcgcggtga ggtcgtcggg ccgcagttgc
     1321 atcatggaac ccttgtgggc acgcccgatc ggcgaccgtg cggtcgcgac gatgacggcc
     1381 tcgggcatcg gggtctccgc ttgtctggcg aatggtgcgg tgaacgcagc gtaactcgcg
     1441 ggccactctg tcactcggtt gcggccgctt gcggacggcg gcagctcatc ggcgtcccga
     1501 ttgactaggc tcgaacccgt ggatgttcat gacagcttgc tcgacctcgt cggcgacacc
     1561 ccgctcgttc ggttgtcgag gatcgcggcg cccggcggtg cccaactgct ggcgaagctt
     1621 gagcagctga atcctggcgg ctcggtgaag gaccgcatcg cggtgcggat ggtcgagacg
     1681 gccgaagcat cgggtgcgct gaagcccggt ggcacgatcg tcgaaccgac gtcgggcaac
     1741 accggagtcg gcctcgcgat tgttgcggcg cgcaagggtt accggtgcgt gttcgtgatg
     1801 ccagacaaga tgagtgccga gaaaatcaat gtcctcaggg gttatggcgc gcaggtcgtg
     1861 gtttgcccaa cggcggtcgc gcccgaggat cccgagtcgt actactccgt gtccgaccgg
     1921 ttggtgcgcg agatcgaggg tgcctggaag ccggatcagt acagtaaccc cgacaatcct
     1981 cggtcgcatt acgagacaac ggggcccgag ttgtggcgcc agaccgacgg gcagctcacc
     2041 tgctttgttg ccggcatcgg taccggggga acgatcagcg gcaccggacg gtatctgaaa
     2101 gaggtgtccg gcggcgcggt tcgcatcgtc ggagccgacc cggaggggtc cgtctattca
     2161 ggcggcactg gccggccgta tctcgtcgag ggtgtcggcg aggatttctg gcccaccacc
     2221 tacgaccgca cgatctgcga cgacatcatc gccgtctctg acaaggattc gttcctgatg
     2281 acgcgccggc tggcccgcga tgagggcatc ctcgtcggtg ggtcgagcgg catggcggta
     2341 gtggccgctg cctcggtcgc tgcgtcgctt tcgccgtccg atgtcgttgt ggtgctcttg
     2401 cccgactcgg ggcgcgggta cctgtcgaag atcttcaacg acgagtggat ggccgactat
     2461 gggttcctgg cgggatcgag ttcgaccagc gtcgcggatg tgctgaaacg caaggggaat
     2521 cggctgccgg agatggtgca tctgcatccc accgagaccg tgcgcgaggc gatcgacgtg
     2581 ctgcgtgagt acggcgtttc ccaggtgccg gtcgtgcggg ctgaaccgcc ggtgatgtcg
     2641 gcggaggtca tcggatctgt cgtcgagcgc gacctgctcg acgcgttgtt ctccggacgc
     2701 gccgcgttgt ccgacgcgct ggagaagcac atttctgcgc cactgccgat gatcggcggc
     2761 ggcgagccgg tcgcggcggc gatgactgct cttgaaacgg cggacgccgc tgtggtgctc
     2821 atcgacggaa agccagccgg aatccttacc cggcaagacc tcttgggatt tctcgcgcac
     2881 tgatctgttc tcgcgtcgac ctattgcgcg ttaatgcggg ccgatcatgc tcttgaggag
     2941 ttctagcatt cgctcggggg aagcagcgtg gccctcgtcg tccttcacga cgaccaacgc
     3001 ttccgggacg ttcgccgcga gccattcgcc gtgtgccgcg gggacgagga cgtcttggct
     3061 tccgtagtgg acttccgccg gaatcgcgat ctcagatacg tcgaagcccc acggtctggt
     3121 gaacgcgagg tcgtcgtcga cccaccccca tgggccgttc acgacgcatt ccggtaccgc
     3181 ctcgcgcatc acccgcatga cgagcgggtg ggcgaacacc tcgcgatcag cgtccggcag
     3241 atcccagctc tcgctcatca cgttggccgc gtcagcggtg acccgcgcca ccatcgcgtc
     3301 ggcttccttc gatagctcgc ggtagagggt gttttcgcca gcaagtgccc agccgaactc
     3361 tttgacgttc tctggatcca tgcctgcgaa ccagtcaaag tcggccgtgt cgaatggcac
     3421 gatgcccacc acgcaccgca cgaatgtgac
//
LOCUS       DATCBJ010000137         2636 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_173638, whole genome shotgun sequence.
ACCESSION   DATCBJ010000137 DATCBJ010000000
VERSION     DATCBJ010000137.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2636)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2636)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2636
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_173638"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            224..1858
                     /locus_tag="VII50_02775"
     CDS             224..1858
                     /locus_tag="VII50_02775"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY5049797.1"
                     /translation="MAAASAAAMLATFALPSASSASAARAAAGSVLTMESSPENSITQ
                     DFNPLVQSTGAYQMGADSLIYEPLLQFNLSRPSQAPYYFLATAYKWGTGGKSITFTVR
                     TGVKFSDGSAMTPADVAFSFAMLQKYPDVNTAGIPITGTSVSGNNVTVTFSSAQYTNL
                     QAIGNVYVVPQASWSTVGDPAMYTDANPVGTGPYTFGTFSASGIMLNANPNYWGGTPS
                     IGQVDFPVYASNDTVLSALTTNALDWAGNFLTGLQAAYIAPSPSTHHDYFAAVQTNTL
                     EPNLNVWPTNQLAVRKAISLAIDRTAISTQGEAGLEPVATNASGIVLPGFKNLLVPAV
                     AKDTLSAHANPAAAIKLLKAAGYVIKNGFFSKGGKEVTIPVTDPSAYTDYAEDDALVA
                     QELKAAHINATFVGQSATAWAADLASGKFVLSMHWSQTTISAYQLYDYWLNSALITNN
                     SAGNFERLRDPAINKDLSALAGQTSIKGQIKDLTPIEQYVAQNLPVIPTVYGAAFEEY
                     NSSRFTGFPSASNSYEIGQPGSPQNEVVVLRLKPTS"
     gene            1950..>2636
                     /locus_tag="VII50_02780"
     CDS             1950..>2636
                     /locus_tag="VII50_02780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011718972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HEY5049798.1"
                     /translation="MTATDANGSGGVARPFPEGFLWGVASSAYQIEGAAQSDGRGPSV
                     WDTFSHTPGRVRGGDTGDIACDFYHRSEEDLDLLKELGLGAFRFSISWPRVQPSGRGA
                     VNQRGLDFYRSLVDGLRSRDLEPAITLYHWDLPQALEDAGGWAQRDTAERFADYAQIV
                     AEAIGDVGAMWITLNEPQVVAHQGYRIGTHAPGHTDDALAAAATHHLLLAHGLALERL
                     RDVMPAARLGI"
BASE COUNT          493 a          908 c          836 g          399 t
ORIGIN      
        1 gagggatacc aggaaaggag cccgccagtg acggcatagc ttcgacggcc agtaacccgc
       61 cgccacaagc aaacgcctgc agcggcgggc cctgacaacg atcggcgcgt gccgtacacg
      121 ccccgatcgc aatgcaccga atgatgctct acgaaaggta catcacgcaa tggaaccgaa
      181 aatcctgaag aagcggaggc tgagattcgc ctccgcagcg gctgtggctg ccgccagcgc
      241 ggcggccatg ctcgcgacat tcgcgttgcc gagcgcatcg agcgccagtg ccgccagggc
      301 cgcggcaggc tccgtcctga cgatggagtc aagcccggag aactcgatta cccaggactt
      361 caacccgctc gtccagtcga cgggcgcgta tcagatgggc gccgactcgc tgatctacga
      421 accgctcctg cagttcaacc tgtcacggcc gtcacaggct ccctactact tcctcgcgac
      481 cgcctacaag tggggcaccg gcggcaagtc gatcaccttc acggtccgca ccggcgtgaa
      541 gttctcggac ggctcggcga tgaccccggc cgacgtggcc ttcagcttcg ccatgctgca
      601 gaagtacccc gatgtcaaca cggccggcat tccgatcact ggaaccagcg tgtcgggcaa
      661 caacgtgacg gtcaccttct cgtcggcgca gtacacgaac ctgcaggcga tcgggaacgt
      721 gtacgtcgtt ccccaggcga gttggagcac ggtcggcgac ccggcgatgt acaccgacgc
      781 gaacccggtc ggtaccgggc cctacacgtt cggcacgttc agtgcgtccg ggatcatgct
      841 gaacgccaac ccgaactact ggggtggcac gccgtcgatc ggccaggtgg acttccctgt
      901 ctacgcatcc aacgacaccg tcctctccgc gctgacgacc aacgcgctgg actgggccgg
      961 caacttcctg accggcctgc aggcggcgta catcgcgccg tcgccctcaa cccaccacga
     1021 ctacttcgcc gcggtgcaga ccaacaccct ggagccaaac ctcaacgttt ggccgacaaa
     1081 ccagttggcg gtccgcaagg cgatcagcct cgccatcgac cgtacggcga tcagcaccca
     1141 gggtgaagcc ggactcgagc cggtggcaac gaacgccagc ggcatcgtgt tgccgggctt
     1201 caagaacctg ctcgtgccgg cggtggccaa ggacaccctc tcggcgcacg ccaacccggc
     1261 ggcagcgatc aagctgttga aggccgccgg atacgtgatc aagaacggct tcttctccaa
     1321 gggcggcaag gaggtcacga ttccggtcac cgacccgtcg gcgtacaccg actacgccga
     1381 ggacgacgca ctcgtcgccc aggagctgaa ggcggcgcat atcaacgcga cgttcgtggg
     1441 gcaatcggcg accgcctggg cggccgacct ggcctccggc aagttcgtgt tgtcaatgca
     1501 ctggtcacag accacgatct cggcctacca gctgtacgac tactggctga acagcgctct
     1561 gatcacgaac aactcggccg gtaacttcga gcggctgagg gacccggcga tcaacaaaga
     1621 cctgtcggct ctcgccggcc agacctcgat caagggacag atcaaggacc tgacgccaat
     1681 cgagcagtac gtggcccaga acctgccggt catcccgacg gtctacgggg ctgcgttcga
     1741 ggaatacaac tcgtcgaggt tcaccggctt cccgtcggcg agcaactcgt acgagatcgg
     1801 gcagccaggc tcgcctcaga acgaggtagt cgtgctgcgc ctcaagccca cctcctaacg
     1861 caaccggtgc cggcgccccg tccccccggt ttcggggggg cggggtggct gcgcccttcg
     1921 gaacttaaat cggaaaggac aagatcaaca tgacggcgac cgatgcgaac ggctcgggcg
     1981 gggttgcgcg cccgttccca gagggattcc tctggggagt cgcatcttcg gcatatcaga
     2041 tcgagggggc ggcgcagtcc gacggccgcg ggccgtcggt gtgggacacc ttcagccaca
     2101 cgccggggag ggtgcgcggt ggtgacaccg gcgatatcgc gtgcgatttc taccaccgca
     2161 gcgaggagga cctcgacctg ctgaaagagc tcgggctcgg ggcctttcgt ttctcgatct
     2221 cctggccgcg cgtgcaaccg tcggggcgcg gtgcggtcaa ccagcgcgga ctcgacttct
     2281 atcgctcgct cgtcgacggg ctgcggtcgc gcgacctgga gcccgcgatc accctctacc
     2341 actgggacct tccgcaggcg ctcgaggacg ccgggggctg ggcgcagcgc gacacggcgg
     2401 agcggttcgc cgactacgcc cagatcgtcg ccgaggcgat cggggacgtc ggtgccatgt
     2461 ggatcacgct caacgagccc caggtcgtcg cccaccaggg ctaccggatc ggaacgcacg
     2521 ccccgggcca caccgacgac gcgctggccg cggccgcaac tcaccatctg ctgctcgccc
     2581 acggactcgc gctggagcgg ttgcgcgacg tgatgccggc ggcgcgcctc gggatc
//
LOCUS       DATCBJ010000138         2058 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_243760, whole genome shotgun sequence.
ACCESSION   DATCBJ010000138 DATCBJ010000000
VERSION     DATCBJ010000138.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2058)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2058)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2058
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_243760"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..586
                     /locus_tag="VII50_02785"
     CDS             <1..586
                     /locus_tag="VII50_02785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Phenylacetic acid catabolic protein"
                     /protein_id="HEY5049799.1"
                     /translation="TNQVPLCRCSYGPYARAMVRICKEESFHQRQGFDLLLTLSHGTP
                     AQHAMAQDAVNRWWWPSLMMFGPPDTESPNSAQSMAWGIKRFSNDELRQKFIDMTVPQ
                     AKVLGLDLPDPDLELDPQTGHYRFGVIDWDEFARVVKGGGPCNEQRIAHRVAAHEEGA
                     WVRAAAMAYAAKHSANPAAHAAPEGDNNAGEAVA"
     gene            583..870
                     /gene="paaB"
                     /locus_tag="VII50_02790"
     CDS             583..870
                     /gene="paaB"
                     /locus_tag="VII50_02790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003857353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaB"
                     /protein_id="HEY5049800.1"
                     /translation="MSDAGLPLWEVFIRGRRGLSHVHAGSLHAADAAMALRNARDVYT
                     RRSEGVSIWVVRSQDIYASSPDEKDPFFAPAADKVYRHPTFYDLPEEVRQM"
     gene            867..1640
                     /gene="paaC"
                     /locus_tag="VII50_02795"
     CDS             867..1640
                     /gene="paaC"
                     /locus_tag="VII50_02795"
                     /EC_number="1.14.13.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaC"
                     /protein_id="HEY5049801.1"
                     /translation="MSEASGPTAAALFTYALRLGDDALVLSQRLSEWASRAPEIEEDI
                     ALTNIALDLLGQARALLDYAAVVEGGTRDEDDLAYLRNEREFRNVHLVEQENGDFAHT
                     IARQLFFSTYQLFLYEELISSTDELLAGVAAKAVKEAAYHHDHAALWTVRLGDGTDDS
                     RKRMQAAVDRLWPFTAELFDADDVETGLAAKGIAVDPATLRTRWVRAISDTFATATLV
                     IPPTVWTSHGGRRGVHTECFGYLLAEMQYLHRLHPGASW"
     gene            1643..>2058
                     /gene="paaD"
                     /locus_tag="VII50_02800"
     CDS             1643..>2058
                     /gene="paaD"
                     /locus_tag="VII50_02800"
                     /inference="COORDINATES: protein motif:HMM:TIGR02159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaD"
                     /protein_id="HEY5049802.1"
                     /translation="MPVSVTVPISTAIDTTAVEQGWAVSSAVADPELPVVTIGDLGIV
                     RSVDIDAQGCVHVVLTPTYSGCPATAVIRDDVVGALHEAGFDRVEVRTVLAPAWTTDW
                     ITDAGRRKLADNGIVPPSGVGEQRRVDLALAVRCPR"
BASE COUNT          355 a          651 c          682 g          370 t
ORIGIN      
        1 caccaaccag gtgcccttgt gtcgctgctc ctacggtcct tacgcgcgcg cgatggtacg
       61 gatctgcaag gaagagtctt tccaccagcg acagggcttc gacctccttc ttacgttgtc
      121 gcacggcacg ccggcacaac acgcgatggc ccaagatgcg gtgaaccgct ggtggtggcc
      181 gtcgttgatg atgttcgggc cacccgacac cgagtcgccg aacagcgcgc agtcgatggc
      241 gtgggggatc aagcggttct ccaacgacga gctgcggcag aagttcatcg atatgacggt
      301 gccgcaagcg aaggtactgg gtctcgatct gcctgaccca gacctcgagc tcgatccaca
      361 aacgggtcac taccggttcg gtgtgatcga ttgggacgag ttcgctcgcg tcgtcaaggg
      421 cggcggaccg tgcaacgagc agcgcatcgc gcaccgggtc gccgcccacg aggagggtgc
      481 ctgggtgcgt gccgcggcga tggcgtacgc cgccaaacac agcgctaacc ccgcggctca
      541 cgccgcgccg gaaggcgaca acaacgcggg ggaggcggtc gcatgagcga cgcgggtttg
      601 cccttgtggg aggtgttcat ccgcggtcgg cgagggcttt cacacgtcca tgccggcagc
      661 ttgcacgcag cggatgcggc gatggcgctg cgcaacgcgc gcgacgttta cacccgccgg
      721 agtgagggtg tgagcatctg ggtagtgcga tcgcaggata tttatgcctc gagtcccgac
      781 gaaaaggacc cgttcttcgc acctgccgcg gataaggttt atcgacaccc aacgttttac
      841 gatctgcccg aggaggtgcg acagatgtga gcgaggcaag cggtcccacg gcagcagccc
      901 tcttcaccta cgcgctgcgc ctcggtgacg atgcgctcgt gctgtcgcag cggctgtcgg
      961 agtgggcatc tcgcgcgccc gagatcgaag aggacatcgc gctcacgaac attgctctcg
     1021 atctcctcgg gcaagcacgt gcgttactcg actacgcggc agtcgtcgaa ggtggtactc
     1081 gcgacgagga cgacctcgcc tatctgcgta acgagcgcga atttcgcaat gtgcatcttg
     1141 ttgaacagga gaatggcgac ttcgcacaca cgattgcgcg ccagttgttc ttcagtacct
     1201 atcagctgtt cctctacgag gagctgatca gctcaaccga cgagctactc gccggtgtgg
     1261 cggcgaaggc cgtcaaggag gccgcgtacc accacgacca cgcagctctt tggaccgtgc
     1321 gtctgggcga cggtaccgac gacagccgca aacggatgca agctgctgtc gaccggctct
     1381 ggccctttac ggcggaactt ttcgatgccg acgatgtcga gacgggcctc gcggcgaagg
     1441 gcatcgcggt cgatccggca acgcttcgga cccgatgggt tcgcgcgatc agcgatacgt
     1501 tcgcgacggc gacgttggtg atcccgccga cggtctggac gtcgcacggc ggccgccgtg
     1561 gcgtgcacac agaatgcttc ggctacctcc tggcggagat gcaatacctg caccggctgc
     1621 atcccggcgc ctcatggtga cggtgccggt ttcagtcacg gtgccgattt caactgcgat
     1681 cgacacgacg gccgtcgagc aggggtgggc ggtgtcgtcg gcggttgccg atcccgaact
     1741 gcctgtcgtg accatcggcg atctcggaat cgtgcgcagc gtcgacatcg acgcacaggg
     1801 ttgcgtgcac gtcgtcctga ctcccaccta ttccggctgc ccggctaccg ccgttatccg
     1861 cgacgacgtc gtcggtgcct tgcacgaggc gggcttcgat cgggtcgagg tgcgcaccgt
     1921 gctcgcgccg gcgtggacca ccgactggat caccgatgcc ggacgccgca agctcgccga
     1981 caacggcatc gtgccgccgt ccggggttgg cgaacagcgg cgggtcgatc tcgcgcttgc
     2041 tgtgcgttgc ccgcggtg
//
LOCUS       DATCBJ010000139         2953 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_132796, whole genome shotgun sequence.
ACCESSION   DATCBJ010000139 DATCBJ010000000
VERSION     DATCBJ010000139.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2953)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2953)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2953
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_132796"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            76..1596
                     /locus_tag="VII50_02805"
     CDS             76..1596
                     /locus_tag="VII50_02805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent malic enzyme"
                     /protein_id="HEY5049803.1"
                     /translation="MSDLQADPDTARGGRPTGAGYTVTMRVLAPHDPTLLPRLAEIVS
                     DARGVVTGMDLVDVTTTGATVDLTMIALDEGHVDEVAAALETQAGVKVRHTSDPTFLY
                     HLGGKIQVTARTQLRTRQDMSMAYTPGVARIASAIAVRPGSAWTLTAKGTSVAIVTDG
                     TAVLGLGNIGPLGALPVMEGKSLIFKEFGGVNAYPLCLNATSPQAVIDVTLAIAPGFG
                     GINLEDIAAPACFQIETELQRQLDIPVFHDDQHGTAVVVSAALVNACRITGRTFPDVR
                     VVHVGCGAAGTAICSALLDAGVKDLIIFDKDGVLRERADTPPHHRVLAARSNPRGITT
                     MEEALRDADVFIGTARRGSVEPDLLLAMAPHPIVFALSNPEPELLPEEARKDALLATG
                     RSDMPNQINNALCFPGFFRGALDARATSVTLAMKQAATRAIADIVTDDQRGLGVIIPT
                     MFQTRLHQAVARAVSDAWLAEHPDGRKRDAFMLAEPGADFGMPDLPAHIPSDDRAS"
     gene            1593..2627
                     /gene="moaA"
                     /locus_tag="VII50_02810"
     CDS             1593..2627
                     /gene="moaA"
                     /locus_tag="VII50_02810"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HEY5049804.1"
                     /translation="MTKPRFTAQPIDTRARPLRDLRISVTDRCNLRCRYCMPREVFGP
                     GFVFLPRTELLHFEEIARVVGAFARAGVTKVRLTGGEPLLRAGLADLVAMVAAVPGVD
                     DIALTTNGSLLARHAERLRTAGLTRITVSLDTLDEETFAKVADSEVTLSSVLDGIAAA
                     ATAGFAPLKLNAVIRRNWNEADVEGLAAYARERGHTMRFIEYMDVGDTNGWRLDDVVP
                     AAEIVDRIAAVWPLEVVSPGYPGEVANRYRYLDGSGEVGVISSISKPFCGTCTRARLS
                     AIGEVYTCLFATTGHDLRALLRSGGSDADLDAAIAGIWTARADRYSELRAAHASHEGP
                     HKVEMSYIGG"
     gene            2678..>2953
                     /gene="orn"
                     /locus_tag="VII50_02815"
     CDS             2678..>2953
                     /gene="orn"
                     /locus_tag="VII50_02815"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000175 - 3'-5'-RNA exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HEY5049805.1"
                     /translation="MSDYLVWIDCEMTGLSLRTDALIEVALLMTDAELNVVGDGVDLV
                     IKPPQEALDTMPDVVRAMHTGSGLLDELGAGITLAEAEEQLLVVVRQY"
BASE COUNT          494 a         1019 c          965 g          475 t
ORIGIN      
        1 agtcgctgag cgcggtgcgc ccggctgtgg tgatcaccac agtcccgacg ccgttcacct
       61 gcggcatgat gaagcatgag cgatttgcag gccgacccgg acacggcacg aggtggtcgg
      121 ccaacgggtg ccggttacac ggtcaccatg cgggtgctgg ccccgcacga tccgacgctg
      181 ctcccccgac tcgccgagat cgtcagcgac gctcgaggcg tcgtcaccgg gatggacctc
      241 gtcgacgtca cgacgacggg cgcgaccgtc gacctcacca tgatcgccct cgacgaaggc
      301 cacgtggatg aagtcgcagc ggcgctcgag acccaggccg gcgtcaaggt ccggcacacc
      361 tccgacccga ccttcctcta tcacctgggc ggcaagatcc aggtcaccgc gcgcacccaa
      421 ctgcgcaccc gccaggacat gtcgatggca tacacccccg gcgtggcaag gattgcctcg
      481 gcaatcgccg tccggcctgg gtccgcgtgg acactcacgg ccaaaggcac gtcggtcgcg
      541 atcgtgaccg acggcaccgc cgtccttgga ttgggcaaca tcggcccgct cggcgcgctg
      601 ccggtcatgg agggcaagtc gctgatcttc aaggaattcg gtggcgtgaa cgcctacccg
      661 ctgtgcctga atgcgacgag tccgcaggca gtgatcgacg tcacgctggc gatcgcgccc
      721 gggttcggcg gcatcaacct cgaagacatc gcggcgcccg catgctttca gatcgaaacc
      781 gagttgcagc gacaactcga tatcccggtc ttccatgacg accagcacgg caccgccgtc
      841 gtggtctcgg cggcactggt caacgcgtgc cgcatcaccg gccgcacgtt ccccgacgtc
      901 cgcgtcgttc atgtcgggtg cggtgcagcc gggacggcga tttgttctgc gctgctcgac
      961 gccggcgtca aagacctgat catcttcgac aaggacggcg tgctgaggga gcgcgccgac
     1021 accccgccgc accaccgggt cctcgcggcg aggtcgaacc cgcgcggcat caccacgatg
     1081 gaagaagcac tgcgcgatgc cgatgtcttc attggcaccg cccgccgagg ttccgtcgag
     1141 cccgacttgc tgcttgcgat ggccccgcac ccgatcgttt ttgcgttgtc gaaccccgaa
     1201 ccggaattgt tgccggaaga ggcacgcaag gacgcactgc tggcaaccgg ccgctccgac
     1261 atgcccaacc agatcaacaa cgcgctgtgc ttccccggct tcttccgcgg tgcacttgat
     1321 gcgcgggcga cctccgtgac gctggcaatg aagcaggccg cgacgcgcgc gatcgccgac
     1381 atcgtgaccg acgaccagcg tgggctcggg gtcatcattc cgacgatgtt ccagacgagg
     1441 ctgcaccagg cggtcgcacg cgcggtaagt gatgcatggc ttgccgaaca tccggacggg
     1501 cgcaagcgcg acgcgttcat gctcgccgag cccggcgccg acttcggcat gccagacctg
     1561 ccggcccaca tcccgagtga cgacagggcg tcgtgacgaa accccgcttc accgcacaac
     1621 ccatcgacac tcgcgcccga ccgttgcgcg atctgcgcat ctctgtcacc gaccgctgca
     1681 atctgcgctg ccgctactgc atgccgcgcg aggtcttcgg ccctggattc gtgttcctgc
     1741 cgcgcaccga actgctgcac ttcgaggaga ttgcccgggt ggtgggcgcg ttcgctcgcg
     1801 ccggagtcac caaggtccga ctcaccggcg gcgaacctct cttgcgagcc ggcctcgcag
     1861 atctggtcgc gatggtcgcg gccgtgcccg gcgtcgacga catcgccctg accacgaacg
     1921 gctcattgct cgcgcggcat gccgagcgac tgcggacggc ggggctgacc cgcatcaccg
     1981 tcagtctcga cacactcgac gaagagacgt tcgcgaaggt cgctgactcc gaagtgactt
     2041 tgagttcggt gctcgacgga atcgccgcag cggcgacggc tggcttcgca ccgctgaagt
     2101 tgaatgcggt gatccgccgc aactggaacg aggcggacgt cgaggggctg gccgcttacg
     2161 cccgcgagcg tggccacacc atgcggttca tcgaatacat ggacgtcggc gacaccaacg
     2221 gctggcggct cgacgacgtg gtgccagccg ccgagatcgt cgaccgcatc gccgccgtct
     2281 ggccgctcga ggtggtgtca ccgggttacc cgggcgaggt cgcgaaccga taccgctacc
     2341 tcgacggcag cggggaggtc ggcgtcatct cctcgatcag caagccgttc tgcggcactt
     2401 gcacgcgagc caggctaagc gcgatcggtg aggtctacac ctgcctgttc gccacgaccg
     2461 gccacgacct gcgagcgctg ctgcggtcgg gcggctcgga cgccgacctg gatgccgcta
     2521 tcgccggtat ctggacggcg cgtgccgacc gctactccga actgcgcgcc gcgcacgcct
     2581 cgcacgaggg cccgcacaag gtcgagatgt cctacatcgg aggctgatcg atgcccgcgc
     2641 gtccggtgcg ccgactgtga agatctagag tgggcctgtg agcgactatc tggtgtggat
     2701 cgactgtgag atgaccggcc tgtccctgcg gaccgacgcc ctcatcgagg tcgccctgct
     2761 gatgaccgac gccgagttaa acgtggtcgg ggacggcgtc gacctggtga tcaagccgcc
     2821 gcaggaggcg ctcgacacca tgcctgacgt ggtgcgcgcg atgcacacgg gttcgggcct
     2881 gctggacgag ctcggggcgg ggatcacgct ggccgaggcg gaggaacagt tgctcgtcgt
     2941 cgtccgccag tat
//
LOCUS       DATCBJ010000140         2831 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_60173, whole genome shotgun sequence.
ACCESSION   DATCBJ010000140 DATCBJ010000000
VERSION     DATCBJ010000140.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2831)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2831)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2831
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_60173"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(46..726)
                     /locus_tag="VII50_02820"
     CDS             complement(46..726)
                     /locus_tag="VII50_02820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009085782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEY5049806.1"
                     /translation="MSEQADGTQLGKQITVFLLDDHEVVRRGVRDMVETAPDLTVIGE
                     GSTAAEALAAVPLLRPDVAVLDVRLPDGDGVTVCRELRSQMPDLVCLMLTSFADDEAL
                     FEAIMAGASGYVLKQIRGGDLVNAIRTVASGQSMLDAGTTAQVMERLRTGTRTEDPLS
                     ALTEQERRILDLIGEGLTNRQIGERLFLAEKTVKNYVSSLLGKLGMQRRTQAAVMVSR
                     VHAREDMH"
     gene            complement(875..1300)
                     /locus_tag="VII50_02825"
     CDS             complement(875..1300)
                     /locus_tag="VII50_02825"
                     /inference="COORDINATES: protein motif:HMM:NF026165.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HEY5049807.1"
                     /translation="MPEIAAVGAIAVRDGALLLIRRAHAPSRGRWSLPGGRVEPGETP
                     QQALVREMAEETGLTVDVGELVGEVVRPGADGVTYRIQDFRVTVVGRHAVAGDDAVAG
                     DDAVAGDDALDVAWVPLEGVPKYRLSAGLLGTLRGWSVI"
     gene            1473..2153
                     /locus_tag="VII50_02830"
     CDS             1473..2153
                     /locus_tag="VII50_02830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6758 family protein"
                     /protein_id="HEY5049808.1"
                     /translation="MTDQPTCPRCGSAVHPPGIWSSDWSCGEHGPVHPLQPAAKQPGP
                     EGLDHVRAHAKVPLWMPSPLPTGWVVTGYTHAGDQRTGAVATLVGCSGPAPLGGAADL
                     VLVAESPGVGLGARLAGLPGPDPGDGFDDGPAHAKLDAAGHPTAMWSLDVGPDNAVFV
                     GEAKGAWIWAILWPSAAGVLLLENLGLDDLVERTEVSPGHLGLDIPHGALMPRLAGLA
                     ASATGPRR"
     gene            2214..>2831
                     /locus_tag="VII50_02835"
     CDS             2214..>2831
                     /locus_tag="VII50_02835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HEY5049809.1"
                     /translation="MRIDLHVHSNASDGTDPPGDLVRKAWRLALDGAPLDGIALTDHD
                     TIAGYDEAAAALEALVGRPERPFVVVPGAEISCILGDVSLHLLGYLFDPADPPLAGEL
                     ELLRTDRIRRAKAMVDKLVELGAPVTWPEVSAIAGDGAVGRPHIAQALVDAGVVADIP
                     AAFVPEWIANDGRAYVEKYSLAPARAIALVRDAGGVTVFAHPAASS"
BASE COUNT          457 a          964 c          924 g          486 t
ORIGIN      
        1 cgacgggtcc tgtcgcgcta agtgcggggc tgcagcgctg ccagttcagt gcatgtcttc
       61 gcgtgcgtga actctgctca ccatcaccgc ggcctgggtg cgccgttgca tgccgagctt
      121 tcccagcaag ctggacacat aattcttgac cgtcttctcc gcgaggaaga ggcgttcacc
      181 gatctggcgg ttggtcaacc cttcaccgat cagatcaagg atgcgccgtt cctgttcggt
      241 caatgccgac agtggatcct cagttcgggt gccggtgcgc aatcgctcca tgacctgcgc
      301 ggtcgtgccg gcatcgagca tcgactgccc gctcgcgacg gtgcggatcg cattcaccaa
      361 gtcgccgccg cggatctgtt tcagtacgta gccagacgca ccggccatga tggcctcgaa
      421 caaggcctcg tcgtcggcga acgaggtgag catcagacag acgaggtcag gcatctgtga
      481 acgcaattca cgacacacgg tcaccccgtc accatcaggc aatcggacgt cgagcaccgc
      541 gacgtccggt cgaagtaacg gaacggccgc caacgcttcg gcggcggtcg acccttcgcc
      601 gatgaccgtc aggtctggtg cggtctcgac catgtcgcgc accccccggc gcacaacctc
      661 gtggtcgtcg agcaggaaca cggtgatctg tttcccgagc tgtgtgccgt cggcttgctc
      721 gctcatttga cttccccaag tccggcgccc cgcttcgcgc caccaatcgc cccgcttagc
      781 gccaccaaat tgcgcaggcg cttgcgcccc taatggtaga gcctacgagg tttgggtgct
      841 ttgcggcgtc tgatgaccaa atcccttccg gctgttagat gaccgaccac cctcgaagtg
      901 tccccaggag cccggcgctg agccgatatt tgggcacgcc ctcgagcggg acccatgcga
      961 catccagtgc atcgtcacca gcgactgcgt cgtcaccagc gactgcgtcg tcaccggcga
     1021 ctgcgtgacg accgacgaca gtgacccgaa aatcctggat ccggtaggtg accccgtccg
     1081 ctccggggcg gactacctca ccgaccaatt cgccgacgtc gactgtgagt cccgtttcct
     1141 cggccatctc acggaccagg gcctgctgcg gcgtctcgcc gggctcgact cgaccacccg
     1201 gcagtgacca gcggccgcgg gacggggcgt gagcgcgccg gatcagcagc agggcgccgt
     1261 cgcggacggc gattgcgccc accgcggcaa tctcaggcac cgccgaagcc taatggccag
     1321 tagcgcttta ctccatttgg gccaatttgt ccctagcgag cgggaatctc ttgacggcga
     1381 acacttgcca gggcacggtc gacgtacgaa ggagtcgacc caacggagtg gtcgaggtac
     1441 agaggagtcg accagatgaa gggttcgacg gtatgacgga tcagccgacc tgccctcgtt
     1501 gcggtagcgc ggtccatccg cccgggatct ggagcagcga ttggagctgc ggcgagcacg
     1561 gcccagtgca ccccttgcag ccggccgcca aacaacccgg gcccgagggc ctcgaccatg
     1621 tgcgagcgca cgcgaaggtt ccgttgtgga tgccgtctcc gctgccgacc gggtgggtgg
     1681 tcacgggcta cacccacgcc ggcgatcaac gcaccggcgc cgttgccacc ctcgtcggct
     1741 gttccggacc ggcgccgctt ggcggcgcgg ccgacctcgt gctggtggcg gagtcgcccg
     1801 gtgtcggcct cggcgccagg ctcgccggcc ttcccgggcc cgacccgggc gacggtttcg
     1861 acgacggacc tgcccacgcc aagcttgacg ctgccggaca cccgaccgcg atgtggagcc
     1921 tcgacgtagg tccagacaat gcagtcttcg tcggggaagc caaaggggca tggatctggg
     1981 cgattctctg gccgtccgcc gccggcgtcc tgctcctgga aaacctcggt cttgacgacc
     2041 tggtcgagcg caccgaggtc agccctggtc acctcggtct cgacatcccg cacggggcat
     2101 tgatgcccag gctcgccggg cttgcggcgt cggcgaccgg gccgcgccga tagcctgagt
     2161 gaggctggtc ggccggccac ccgacgtcga cgatgaggac ggcgcgagcc cgcatgcgga
     2221 tcgacctcca cgtgcacagc aacgcctccg acggcaccga tcccccgggg gatctcgtcc
     2281 gcaaggcgtg gagactcgcc ctcgacggcg cgcctcttga cggcatcgcg ctgaccgacc
     2341 acgacacgat cgctggctac gacgaggccg cggctgcatt ggaagcactc gtcgggcgtc
     2401 cggaacgtcc gttcgtcgtc gtccccggag ccgagatctc gtgcattctc ggtgacgtca
     2461 gcttgcacct gctcggctac ctattcgacc ctgccgatcc gccgctcgcg ggcgagttgg
     2521 agctgttgcg aaccgatcga atcaggcgcg cgaaggcgat ggtcgacaag ttggtcgagc
     2581 tcggcgctcc tgtcacgtgg ccggaggtta gtgctatcgc aggggacggc gcggtgggcc
     2641 gtccgcacat cgcgcaggcg ctggtggacg caggagtggt tgccgacatt cctgctgcgt
     2701 tcgttcccga gtggattgcc aatgacgggc gggcctacgt cgagaagtac tcgttggcgc
     2761 cagcgcgagc gatcgccttg gtgcgggacg ccgggggagt gacggtattc gcgcaccctg
     2821 ccgcgtcgtc g
//
LOCUS       DATCBJ010000141         3688 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_60163, whole genome shotgun sequence.
ACCESSION   DATCBJ010000141 DATCBJ010000000
VERSION     DATCBJ010000141.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 3688)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3688)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3688
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_60163"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1325)
                     /locus_tag="VII50_02840"
     CDS             complement(<1..1325)
                     /locus_tag="VII50_02840"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HEY5049810.1"
                     /translation="MGHRRALIAIVGGSLVLAACTSSTHAAPAVSAPGTSVPASAAAS
                     SATAVGSSSAPVSAGAAASSAVAARSSAASASPSATAAAFPTAIWPTYHHDVARTGNA
                     GTVPAVTGLKVAATASLDGAVYASPLVLHDAKGDLIIAATENNTLYGLRNNGSVVWSR
                     HIGTPVNGASLPCGDISPSGITSTPVYDATSGAVFAVAYLSGGKHELVAANAETGAVA
                     WTAPVDAPGSTVSVEQQRGALTLADGRVWIAYGGLYGDCGPYHGYLVGVPTSGVGADI
                     VYRDPGTREGGFWGPTGPAVDTSGNLYVPVGNGAATSPSDKYDLSDGVIEVTPAGKAA
                     SYFAPPSWASDNAHDYGTTGVVLLPTGQLFINGKSGIAYLLKAGELGGITNSVPSVAV
                     CSAFGGPAYANGIVYLPCTDGLRAVRVTATSLVTLWHADPAGTPIVGGGV"
     gene            1384..1887
                     /locus_tag="VII50_02845"
     CDS             1384..1887
                     /locus_tag="VII50_02845"
                     /inference="COORDINATES: protein motif:HMM:NF016840.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEY5049811.1"
                     /translation="MFQRVPFTGDEKTSLQIALQRHRDVVLWKLEGLDDEQLRRHMTP
                     SGTGLLGLVKHLAAVEYGWFCSTFGRETEPMPFDEQEPDADLRIGPDETTADVVEFYE
                     RACRAADQAIDEVGLDELGTAWFGDQVSMRWVLIHELEETARHAGHMDILRELIDGAT
                     GDHNRPD"
     gene            complement(1904..2155)
                     /locus_tag="VII50_02850"
     CDS             complement(1904..2155)
                     /locus_tag="VII50_02850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5049812.1"
                     /translation="MSRMIVAVAPLKAAVQVAHAAQLALGELSDVGGWRTTGFGVRLV
                     RPDVEQWAQFSASARVVVRDAGFTEIPPDTQTAIALWDD"
     gene            complement(2690..2884)
                     /locus_tag="VII50_02855"
     CDS             complement(2690..2884)
                     /locus_tag="VII50_02855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5049813.1"
                     /translation="MTVVHAGAQVRAFVPGPTDAVPPQIGKLQVAGLGLEPGTPSSAA
                     PGILPVAVTPHVTMSPDNVS"
     gene            complement(2881..3141)
                     /locus_tag="VII50_02860"
     CDS             complement(2881..3141)
                     /locus_tag="VII50_02860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5049814.1"
                     /translation="MSEYRDPPGEAPWAMPIVVRAEKGAEPTEAGVAEAAATAVMQLL
                     DDPRSIDGEWHDEVQRWLEAGSARSRGGLGARSGTRLWSSRV"
     gene            3217..>3688
                     /locus_tag="VII50_02865"
     CDS             3217..>3688
                     /locus_tag="VII50_02865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HEY5049815.1"
                     /translation="MSDQPVGDDVEPTAAQLRERIEAGGAAKYHEANASKGKMFARDR
                     VRALVDAGSFVEDGAFANVLAADLPADGVVTGTATIDGRPVALLANDSTVKAGSWGAR
                     SVEKMIRIAEFAYNAGIPMVYLVDSAGARITDQVAMFPGRRGAGHLFHTQVRASG"
BASE COUNT          629 a         1259 c         1233 g          567 t
ORIGIN      
        1 acgccaccgc cgacgattgg cgtgcccgcc gggtcggcgt gccaaagagt gaccaacgat
       61 gtggcagtca ctcgcaccgc tcgcaagccg tcggtgcacg gcaggtagac gatgccgttc
      121 gcgtacgccg ggccaccgaa cgcgctgcac acggcgacgc tcggaacgga gttggtgatg
      181 ccgcccaact cacctgcctt gagcaggtac gcgatgccac tcttgccgtt gatgaagagc
      241 tgtccagttg gcagcagcac cacgcccgtc gtgccgtagt catgcgcgtt gtcggacgcc
      301 caactcggcg gcgcgaagta cgacgccgcc ttaccggccg gtgtgacctc gatgactccg
      361 tcgctcaggt cgtacttgtc actaggacta gtcgcggcgc cattgcccac tggaacgtag
      421 aggttgccgg acgtgtcgac cgcgggccca gtcggacccc agaacccgcc ctcgcgggtg
      481 ccggggtcgc ggtacacgat gtctgcgcct acccccgagg tcgggacacc gaccaggtag
      541 ccgtgatacg gcccgcaatc gccgtacaga ccgccgtacg cgatccacac ccggccgtcg
      601 gcgagcgtca gggctccgcg ttgttgctcg acgcttacgg tcgagccggg tgcgtcgacg
      661 ggtgccgtcc aggccaccgc gccggtctcg gcgttggctg cgaccagttc gtgcttgccg
      721 ccacttaagt acgcgaccgc gaacaccgca cctgacgtcg cgtcgtacac cggggtgctc
      781 gtgatgccgc tcggactgat gtcgccgcac ggcaagctcg cgccgttgac cggagtgccg
      841 atgtgccgcg accacacgac gctgccgttg ttgcgcagcc cgtaaagcgt gttgttctca
      901 gtcgccgcga tgatcaggtc gcccttcgcg tcgtgcaaca cgagcgggct cgcatacacg
      961 gccccgtcca gactcgccgt cgccgcaacc ttgagacccg tcacagccgg gacggtgccc
     1021 gcgttgcccg tgcgtgccac atcgtggtgg tacgtcggcc agatcgcggt cggaaacgcg
     1081 gcggcggtcg cactcggaga tgccgaagcc gcggacgaac gtgcagccac agctgacgac
     1141 gcggccgcgc ccgcggacac cggcgcggac gacgagccaa ccgctgtagc ggacgacgca
     1201 gccgcggagg ctggcactga cgtaccgggc gcggatacgg ccggtgccgc gtgcgtgctg
     1261 gacgtgcacg ccgcgagtac caggctgccg ccgacgatgg ctatgagcgc gcgtcgatgt
     1321 cccatagcgt ccagtgtgcc cacatctggg ccgatcacca cgtcgcagtg agaggatcca
     1381 cgcatgttcc agcgagttcc gttcaccggc gacgaaaaga ccagtctgca gatcgccctc
     1441 caacgccatc gcgatgtcgt gctctggaaa ctcgagggct tggacgacga gcagctgcgg
     1501 cgtcacatga cgccgtcggg caccggcctg ctcggattgg tgaagcacct tgctgccgtc
     1561 gagtacggct ggttctgctc gacattcgga cgcgagaccg aaccgatgcc gttcgatgag
     1621 caagagcccg acgcggatct acgcatcgga ccggacgaaa cgaccgctga cgtcgtcgag
     1681 ttctacgagc gtgcatgtcg ggccgccgac caagccatcg acgaggtggg gctcgatgag
     1741 ctcggcaccg catggttcgg cgaccaggtc tcgatgcggt gggtgctgat ccacgagctc
     1801 gaggagaccg cccggcacgc cgggcacatg gacatcctgc gtgaactgat cgacggcgcg
     1861 accggcgacc acaaccgccc cgactgatcg ggcgacccga gcgtcagtcg tcccacagcg
     1921 cgattgctgt ctgggtatcg ggcgggatct cggtgaagcc cgcgtcccgc accacaacgc
     1981 gagcggacgc cgagaactgc gcccactgct cgacgtccgg ccgtacgagg cgaacgccga
     2041 agcccgtcgt gcgccaacca ccgacgtcgc tcaattcacc cagggccagc tgcgccgcgt
     2101 gcgcgacctg caccgctgcc ttcagcggtg cgacggcgac gatcatgcgg ctcacgaatc
     2161 tcgctctgag agcggcagct catccctcgc cggcgagtct aagcgtcata aggtcggcga
     2221 tcgctaagag cggccaaggg gttccgtgct gcgaatccga cgccaaggct ccctgcgtct
     2281 aggggcagtg ctggcggtcg gtcttgtcga gcgagtggcc gattggtgcc ggccgatgcc
     2341 ctcgcgccca gagtacaaat ccatagccga ggccagcagc gagcggaatg ctgacagatg
     2401 gaccgagcac accccaacga gccgtcgctg ctgcgccaac ggcgaaaccg acgccgagga
     2461 cagcgactga accggcgact gtcgccgccc accagcctcg ccaagtcctc atccccggcc
     2521 tcccgcccag acgacagcac tgccccagga tttcttcgct cgaactgtac cggcgtcctt
     2581 cgctgctagc gcagtcacag catcactgtc ggcgctggta tcgacgatgc cgagagatag
     2641 gcccaatgcc tcggccaact gttgccgcga gggcggccga gatatgcctc taagagacat
     2701 tgtccgggct catcgtgacg tgcggagtaa cggcgacggg cagaatgcca ggggcggcgg
     2761 aggacggggt gccgggctcg agaccaaggc cggcgacctg gagcttgccg atctgcggcg
     2821 gcacggcgtc ggtcgggccc gggacaaacg cgcgcacctg agcaccggca tgaacgacgg
     2881 tcacacccgg gagctccaaa gccgcgtccc acttcgagcc ccgagcccgc cgcgcgacct
     2941 tgcggatccg gcctcgagcc agcgctgaac ctcgtcgtgc cactcgccgt cgatgctgcg
     3001 cggatcgtcg aggagctgca tcaccgctgt cgccgcggct tcggcgacgc ccgcctcggt
     3061 cggctcggcg cccttctctg cccgcacgac gatgggcatc gcccacggcg cttccccggg
     3121 cggatcgcga tactcagaca cccgctcatc ctgtctcacc gaagatccgt ctcgtggcct
     3181 gacgcgatcc gcggcggagg tggatacatt cggcggatga gcgatcagcc cgtgggtgat
     3241 gacgtggagc cgacggctgc acagctgcgc gagcgaatcg aagcgggcgg cgcggcgaag
     3301 tatcacgagg cgaacgcctc gaagggcaag atgttcgccc gcgaccgggt gcgggcgctc
     3361 gtcgacgcgg ggtccttcgt cgaggacggc gcattcgcga acgtgctggc tgccgatctg
     3421 ccggccgacg gggtcgtgac gggcaccgca accatcgatg gtcgtccggt tgcgttgctg
     3481 gcgaacgact cgacggtcaa ggccggttcg tggggtgcgc gcagcgtcga gaagatgatt
     3541 cgcatcgcag aattcgctta caacgcaggc attccgatgg tctatcttgt cgactcggcg
     3601 ggcgcgcgga tcaccgatca agtagcgatg tttcccggac gccgaggggc tggccatctc
     3661 tttcacacgc aggtgcgggc aagcgggt
//
LOCUS       DATCBJ010000142         4237 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_206764, whole genome shotgun sequence.
ACCESSION   DATCBJ010000142 DATCBJ010000000
VERSION     DATCBJ010000142.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 4237)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4237)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4237
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_206764"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..899)
                     /locus_tag="VII50_02870"
     CDS             complement(<1..899)
                     /locus_tag="VII50_02870"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY5049816.1"
                     /translation="MLGARSHPPWRGVALLVAGAFFMENLDGTIIATAAPRMADSLGV
                     RPVDLNVVITAYLLTLSVFIPVSGWLADRFGARTIFGTAIAVFTVASGLCAISTSLGE
                     LTGVRVLQGIGGAMMVPVGRLVVLRKTTRAELIDAFAYLTWPALAAPVIAPALGGLLT
                     TYASWRWIFVINLPLGVAALLVTTRIVSNEPHVDQTPMDWQGFALTGVGLATLVTSLE
                     LVAARRVEWFYVGTAGAVAIITIALAVRHLRRTEHPLVELASLRIPTFRVTALGGSLF
                     RLAIGAVPFLLPLMFQDAFGWSPL"
     gene            complement(952..1980)
                     /locus_tag="VII50_02875"
     CDS             complement(952..1980)
                     /locus_tag="VII50_02875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEY5049817.1"
                     /translation="MAVADRGRVTRVTIAAIAAEAGVSVPTVSRVLNGRVDVAADTRT
                     RVEELLEKHDYQRRGLGRGTRANLIDLVFNDLDSPWAVEIIRGVEDVAHDAGVGTVVS
                     AIHNQVTSERRWWQNVQARASDGIILVTSHLQPKLQAEVHRYLPTVVVDPSGSPSYDV
                     PTIGATNWAGGLSATEHLIEFGHRRIGIITGPPRLQCSRARLDGYRAALEAADLQFDH
                     DLVYTGDFYHESGFTGGTQFLGLPDPPTAVFASSDQMAFGVYEAARQHGLRVPSDLSI
                     VGFDDLPEAPWASPPLTTVRQPLAEMGGLAARTVLRLVRGEPIETLRIELSTQLVVRE
                     STAPPRAR"
     gene            complement(2073..>4237)
                     /locus_tag="VII50_02880"
     CDS             complement(2073..>4237)
                     /locus_tag="VII50_02880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HEY5049818.1"
                     /translation="GHLTRPFGTVPVDPAMGARSLARAQADIVAASRHGIPAIAHDEC
                     LSGFMAWGATIYPTALAWGATFDPSLVEIMAAQVGETMRSVGVHQGLAPVLDVTRDYR
                     WGRVEETIGEDPYLVATIGTAYVRGLESAGVVATLKHFVGYSASRAGRNFAPVSAGPR
                     EVADILLPPFEMAIRDGGARSVMHAYTEIDGVPGAANSLLLTDLLRDQWGFDGTVVAD
                     YFGVTFLALHHKVAVDESSAAALALWAGVDVELPSVRCYGQPLINAVESGRLSEEFVD
                     RATRRVLTQKGQLGLLDPDWSPHPPAIMPSQQFGDASDDVRGSIELDAPESRAVARQL
                     AEESIVLVANSGVLPLRRGTRIALLGPRADDAFAMLGCYTFPSHVGLQYPKWPLGLSI
                     PTLLDATRKELPDADVVYAAGCDVDGTDISVFAAAVTAAAAADVCVVALGDRAGLFGR
                     GTSGEGCDAETLELPGVQGQLLEAILATGRPVVLVMVSGRPYALGAYADRLAAVVQSF
                     FPGEEGGAALAGVLSGRICPSGRLPVCIPRSPHGQPGTYLTAQLGLRSGVSTVDPTPQ
                     WPFGHGLSYTTFRWDSVAVDGNAVTETDRVDATTDGAVRISLTVSNVGRRAGTDVVQL
                     YLHDPVAQVTRPDVRLIGYARVDVEPGASRRVSFDVSADLSSFTGVAGVRIVEPGDLQ
                     LRLSTSSSESRHIVNIRLIGPERAVGLSRRMVADVTVE"
BASE COUNT          789 a         1362 c         1409 g          677 t
ORIGIN      
        1 agcgggctcc agccgaatgc gtcctggaac atcagcggta gcaggaacgg caccgctccg
       61 atcgcgagtc gaaacaagga accccccagt gcggtgaccc gaaatgtggg gatccggagc
      121 gacgccagtt ctacgagggg atgctccgtg cgtcgtaggt ggcgaaccgc gagggcgatc
      181 gtgatgatgg ccacggctcc tgccgtgccg acatagaacc actcgactcg gcgggctgcg
      241 acaagttcca ggctggtgac gagggttgcg aggccgacgc ccgtcaacgc aaacccttgc
      301 cagtccatgg gtgtctgatc gacgtgcggt tcattcgaca cgatgcgcgt cgtgacgaga
      361 agtgccgcga cccctagtgg caagttgatc acgaaaatcc atcgccacga ggcatacgtc
      421 gtcagcaagc cgcccagcgc cggtgcgatg accggtgcgg ccaacgcggg ccaggtcaga
      481 tacgcgaacg cgtcgatcaa ctcagcgcgc gtcgtcttgc gcaggacgac gagcctgccg
      541 accgggacca tcatcgcgcc gccgatgcct tgcaagacac gcacccctgt gagttcaccg
      601 aggctggtgc tgatcgcgca caatcccgac gcaacggtga acacggcaat ggcggtgccg
      661 aagatggtgc gggcgccgaa ccgatcggca agccacccgc tcaccggaat gaagacgctc
      721 agggtgagca gataggcggt gatgacaacg ttgaggtcga cgggccggac accgagcgag
      781 tccgccatgc gtggtgccgc ggtggcaatg atggtgccgt caaggttctc catgaagaac
      841 gcgcccgcga ccaggagcgc cacgccacgc cacggcggat gactgcgggc gccgagcatg
      901 cccgacagtc tgtcaacgca acgccaagcg ctcttcgtcg ggcggcaatg cctaccgtgc
      961 gcgcggcgga gcggtgcttt cgcggacgac gagctgggtt gaaagctcta tccgcaaggt
     1021 ctcaatcggt tcgccacgca ccagacgaag aactgtccgt gcggcgagac cgcccatctc
     1081 cgcaagcggt tgacgcacgg tggtgagtgg gggagacgcc cagggtgcct ccggcaggtc
     1141 atcgaacccg acgatgctga ggtcgctggg cacccgaaga ccatgttgcc gggctgcctc
     1201 atagacgccg aacgccatct ggtcactcga ggcgaacacc gccgttggcg ggtcaggcaa
     1261 tcccaagaac tgtgtcccgc cggtgaagcc cgactcgtgg tagaagtcgc cggtgtagac
     1321 aaggtcgtga tcgaactgca ggtcggcggc ttcaagtgcg gcgcggtaac cgtcgaggcg
     1381 cgcgcgactg cattgcagcc gcggtggccc ggtgatgatt cctatccgcc gatgtccgaa
     1441 ctcgatcagg tgctcggtcg cgctgagtcc gccggcccag ttcgtcgccc cgatcgtcgg
     1501 cacgtcatat gacgggctgc ccgagggatc gacgacgact gtcggcaggt agcggtgtac
     1561 ctctgcttga agtttcggct gcagatgtga cgtcaccaag atgatgccgt cggacgcccg
     1621 ggcctgaacg ttttgccacc atcgacgctc cgacgtgact tggttgtgga ttgccgagac
     1681 gacagttccg acgccggcgt cgtgagcgac atcctcgacg ccgcgaatga tttcgacggc
     1741 ccacgggctg tcgagatcgt tgaacacgag gtcgatgagg ttcgctcggg ttcctcgtcc
     1801 gaggccgcgg cgctggtagt cgtgcttctc gaggagttct tccacccggg tgcgggtgtc
     1861 ggccgcgacg tcgactcgac cgttgaggac ccgagacacc gtcggcaccg agacgcccgc
     1921 ctctgcggcg atggcggcaa ttgttacccg ggtcacccgc cctcgatcag cgacggccac
     1981 aacgagtctc ctttgaaagt cgcgttacac gatacgcaag cggcgccgtg gattcctggt
     2041 gatccacgat tggagatcgc ctgggatcgc cgctactcca ccgtgacgtc ggccaccatg
     2101 cgccttgaca accctacggc gcgctcaggt ccgatcagtc tgatgttcac gatgtgtcgc
     2161 gattcgctgc ttgacgtcga gagtcgaagt tgaaggtcgc ccggctcgac gattcggaca
     2221 ccagcgacgc ctgtaaagga cgagaggtcg gcggacacgt cgaaagaaac ccgccgcgat
     2281 gcgcccggct cgacatcgac gcgggcgtag ccgatcaaac ggacgtcggg tcgggtgacc
     2341 tgggcaaccg gatcgtgcag gtacagctgg acgacgtcgg ttccggcccg gcgtccgaca
     2401 ttggagacgg tgagcgagat tcgcacggcg ccgtccgttg tggcatcgac gcgatccgtt
     2461 tctgtcaccg catttccgtc gactgcgacg ctgtcccagc ggaaggtggt gtacgagagc
     2521 ccgtgaccga acggccactg cggagtcggg tcgaccgtgc tgaccccgct gcgcaggccc
     2581 agctgggccg tcaggtaggt gccgggctga ccgtgcgggc tgcgcggaat gcacacgggc
     2641 agccggccgg agggacagat ccgacccgac agcacgccgg cgagtgcggc gcccccttct
     2701 tctccaggga agaaggactg cacgaccgcc gccaaccgat ccgcgtacgc gcccagcgcg
     2761 taagggcggc cggacaccat cacgagcaca accggccgac cggtcgcgag gatcgcctcg
     2821 agcagttgtc cttgtacgcc gggcagttct aacgtttcgg cgtcgcatcc ttcgccggat
     2881 gttccgcgcc cgaagagccc ggcccgatca ccgagggcta cgacgcacac gtcggcagca
     2941 gcggccgctg tgacggcggc cgcgaagacg ctgatgtcgg tcccgtcgac gtcgcagcct
     3001 gcggcgtaaa cgacgtcggc gtcaggcaac tccttgcgcg tcgcgtcgag tagtgtcgga
     3061 atggaaagcc cgagtggcca cttcggatac tgaagaccga cgtgactcgg aaacgtgtag
     3121 cacccgagca tcgcaaacgc gtcgtcggca cgggggccta gtagcgcgat ccgcgtccca
     3181 cggcgtaggg ggagcacgcc gctgtttgct acgagcacta tcgattcctc ggcgagctga
     3241 cgagcaaccg cgcgcgactc gggcgcgtcg agttcgatcg atccccggac atcgtcggac
     3301 gcgtcaccga actgctggga aggcataatt gccggcgggt gcgggctcca gtctgggtcg
     3361 agcaggccca attgaccctt ctgcgtcagc actcggcgcg tggcccggtc gacgaattcc
     3421 tctgacagcc gacccgactc gacggcgttg atgagtggct gcccatagca gcgcacgctg
     3481 ggcaactcga catcgacgcc ggcccacaac gccaacgcgg cggcgctgct ttcgtcaacg
     3541 gctactttgt ggtgcagggc aagaaaggtg acaccgaaat agtccgccac gacagttccg
     3601 tcaaagcccc attgatcgcg caacaggtcg gtcagtagca gactgttggc cgcgccgggc
     3661 acgccgtcaa tctcggtgta cgcgtgcatc accgagcgcg cgccaccatc gcgtatcgcc
     3721 atctcaaacg gtggcagcaa gatgtccgca acctcgcgcg gacctgcgga gaccggcgcg
     3781 aagttgcgcc cggcgcgcga tgccgaatat ccaacgaagt gcttcaacgt ggcgacgaca
     3841 ccggccgatt caagcccgcg tacgtaagcg gtgccgatcg tcgcgaccag ataggggtct
     3901 tcaccgatcg tctcctcgac gcggccccag cggtagtcac gtgtcacgtc gaggacagga
     3961 gcaagaccct ggtggacgcc gacggagcgc atcgtttcgc caacctgtgc cgccatgatc
     4021 tcgacgagag acggatcgaa tgtggcgccc cacgcgaggg cggtggggta gatggttgct
     4081 ccccacgcca taaaccctga gaggcactcg tcgtgcgcga tagccgggat gccatgccgg
     4141 ctcgctgcga caatgtccgc ttgtgcgcgc gccagtgagc gcgcacccat cgccggatcg
     4201 actgggaccg ttccgaacgg gcgagtcaga tggccga
//
LOCUS       DATCBJ010000143         2230 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_537718, whole genome shotgun sequence.
ACCESSION   DATCBJ010000143 DATCBJ010000000
VERSION     DATCBJ010000143.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2230)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2230)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2230
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_537718"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            83..484
                     /locus_tag="VII50_02885"
     CDS             83..484
                     /locus_tag="VII50_02885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5049819.1"
                     /translation="MSEAPARNHSHPSGHVIPSKTAYVSTDAGGHWRQVAELPQTSGY
                     VFDLAAADANTWVLAEARGTLEVTRDGGSTWHAATSNDVSPSGGEGWGTVTFVSATKA
                     VAVPWTLNGSALAISNDTARTWDLIAFPSGR"
     gene            complement(499..1449)
                     /locus_tag="VII50_02890"
     CDS             complement(499..1449)
                     /locus_tag="VII50_02890"
                     /inference="COORDINATES: protein motif:HMM:NF020039.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HEY5049820.1"
                     /translation="MSSSGIGASAWDGDRSIVRGPDPAIEVIDERFRSLVVGPEIVER
                     LWTGGRWLEGPVWFGDGRYLLFSDIPNNRMLCWSEITGAVTEFRQPSNHSNGNTRDLQ
                     GRLVTCEHLTRRVTRTEYDGSITVLMDSYEGKPLNAPNDVTVHSDGSVWFTDPGWGIE
                     GNYEGVKAVKEIPRYVFRVDADTGEGVPVITDMDRPNGICFSPDESLLYVVDVDQIRV
                     YDMDGASPVNGRVFVAMKGSDGIRTDRDGNVWSAAGWGGPGFDGVHCYSAAGELLGQI
                     HLPEICANFCFGGARRNRLFITASRSLYSVFVEAQGSQRP"
     gene            complement(1455..>2230)
                     /locus_tag="VII50_02895"
     CDS             complement(1455..>2230)
                     /locus_tag="VII50_02895"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY5049821.1"
                     /translation="DPCGRRSMSQRIAVVTGGGSGIGAAVALALAEDAWTVVLAGRHI
                     ETLDETVVRGSWLTGTLEAVPTDVTDEASVRDLFNSVVDAHGRVDLLFNNAGIGAPNV
                     DIDALELDDWNKVVAVNLTGAFLCTREAFRVMRNQQPQGGRIINNGSISAHAPRPHSI
                     AYTATKHAITGLTKSTSLDGRAFDIACGQIDIGNAATDMASVAANGAAQADGSIRPEP
                     LIDVGEVGRAVRYMASLSLDANVASLMVLPPKMPFVGRG"
BASE COUNT          397 a          780 c          684 g          369 t
ORIGIN      
        1 tacgctcaac aagcaccggg gcctcccacg catgtcggat aggccgagca ggcagcccct
       61 gcccggcaac ccacgagatt gcgtgagcga ggccccggcc cgcaaccact cacacccgag
      121 cggtcacgtc ataccgtcta agactgccta cgtgtccacc gacgccggcg gccactggcg
      181 ccaagtcgct gagctgcccc agaccagcgg ctacgtcttc gacctcgccg ccgcagacgc
      241 caatacctgg gtactcgccg aggcccgcgg aacactcgag gtcacccggg acggtggcag
      301 cacctggcac gctgcgacct ccaacgacgt gtcaccatcc ggcggcgaag gctggggaac
      361 cgtcacgttc gtctccgcaa cgaaggccgt ggccgtgccg tggactctca acgggtctgc
      421 tctagcgata agcaacgaca ccgcccgaac ctgggacctg atcgccttcc caagcggacg
      481 ctagacaagc ccaactcgtc agggtcgctg ggatccttgt gcctcgacga aaacgctgta
      541 gagcgagcga cttgcggtga tgaagagccg gttccttctg gctccgccga agcagaagtt
      601 cgcgcatatc tcgggcagat ggatctggcc gagcaactcg cccgcggcgc tgtagcaatg
      661 caccccgtcg aagccgggcc caccccatcc ggccgcagac cacacgttgc cgtcgcggtc
      721 ggtccggatc ccgtcggaac ccttcatcgc cacgaacaca cgtccattga cagggctcgc
      781 gccgtccatg tcatagacac ggatctggtc gacgtcgacg acatacagca gggactcgtc
      841 gggtgagaag cagatgccgt tcggccggtc catatccgtg atgaccggga cgccctcgcc
      901 ggtgtcggcg tcgacgcgaa acacgtaccg cggtatctct ttgaccgcct tgactccctc
      961 gtagttcccc tcgatgcccc acccgggatc ggtgaaccac accgatccgt cagagtggac
     1021 cgtcacatcg ttcggcgcgt tcagaggttt cccctcatag ctatccatca agacggtgat
     1081 gctgccgtcg tactcagtcc tggtcactcg acgggtgagg tgctcacacg tcacgagccg
     1141 gccctgcagg tcgcgggtgt tgccgttgct gtggttcgac ggctggcgga attccgtcac
     1201 agcgccggtg atctcgctcc agcagagcat ccggttgttg gggatgtcgg agaacagcaa
     1261 gtagcgcccg tccccgaacc acaccgggcc ctcgagccag cggccgccgg tccacagtcg
     1321 ctcgacgatc tcgggtccga ccacgagaga gcggaaccgc tcgtcgatca cttcgatcgc
     1381 tggatccgga ccgcgcacga tcgaccgatc gccgtcccat gcactggcgc cgattcctga
     1441 gctcgacacg tcccctaccc ccggcccacg aaaggcatct tcggcggaag caccatcagc
     1501 gacgcaacgt tcgcatcaag cgaaaggctc gccatgtagc gcaccgcacg gccaacctcg
     1561 ccgacgtcta tcaacggctc cggccgaata gacccgtcgg cttgcgccgc gccgttcgcc
     1621 gccacgctcg ccatatcggt ggccgcgttg ccgatgtcga tctgaccgca cgcgatgtcg
     1681 aaggcacgcc cgtcgagaga cgtcgacttg gtcagtccgg tgatcgcgtg cttcgtcgcg
     1741 gtgtaggcga tcgagtgcgg ccgcggtgcg tgtgcggaga tcgagccgtt gttgatgatg
     1801 cggccgccct gcggttgttg gttacgcatc acgcggaacg cctcgcgggt gcaaaggaac
     1861 gcgcccgtga ggttcaccgc gaccaccttg ttccagtcat cgagttcgag cgcgtcgatg
     1921 tcgacgttcg gagcgccgat gccggcattg ttgaacaaca ggtcgacgcg gccgtgggca
     1981 tcgacgacgc tgttgaagag gtcgcgaacg ctcgcctcgt cggtgacgtc agtggggacc
     2041 gcctcgagtg tgccggtcaa ccacgacccc cgaacgacgg tctcgtccaa cgtctcaatg
     2101 tgtcgacctg cgaggacgac ggtccaggca tcctcggcga gcgcgagggc gaccgccgcg
     2161 ccgatcccgc tgccgccgcc ggtcacgacg gcaatgcgct gcgacatcga tctcctcccg
     2221 cacgggtccg
//
LOCUS       DATCBJ010000144         4525 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_464341, whole genome shotgun sequence.
ACCESSION   DATCBJ010000144 DATCBJ010000000
VERSION     DATCBJ010000144.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 4525)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4525)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4525
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_464341"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..643)
                     /locus_tag="VII50_02900"
     CDS             complement(<1..643)
                     /locus_tag="VII50_02900"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEY5049822.1"
                     /translation="MAPRVLIDASAVPADRGGLGRYVDGLLAALAEKDADLAVVCQRV
                     DAERYGRLVPAAQIVAGPASLTNRTARIAWEQTGLPLVAEHVAAKVIHAPNYSVPMRP
                     GRPVVTTIHDATFFSEPDLYPPTRGAFYRAAIRSAVKRSTRCIVPSKATRDELVRLVD
                     ADSTRIDVAYHGVDHDVFRPPTEAEAARISARLGLRGQQYIAFLGAFEPRKNVP"
     gene            775..1767
                     /locus_tag="VII50_02905"
     CDS             775..1767
                     /locus_tag="VII50_02905"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEY5049823.1"
                     /translation="MVGSAATDRSVVATVVVIAWNGAGLIGPCLDAVAGQELADGFVI
                     WVIDNASTDSTPAVVDRHPIGARLVTVSSNLGFAGAAEVAVREATTPFVVVLNQDARP
                     EPGWLAALLAPLTGPDAHRIAAVTSKVIFTADGRLNNTGVIVGRDGYGRDRGFGQPDD
                     GRFAEPEDVFGFSGTAAALRVVAVRDVGSFDPSFFLYYEDTDLSWRLQLGGWRVRYAP
                     DAVVRHVHAASTDVSSKSFAFFNERNRLLMLAKCAPRSLAAKEILRFAGITLVLPIRR
                     LTRVDIPTGHQFRTPTRVHALASFARMLPSALKKRRAVSRICRRPRAEVARDLS"
     gene            complement(1814..4198)
                     /locus_tag="VII50_02910"
     CDS             complement(1814..4198)
                     /locus_tag="VII50_02910"
                     /inference="COORDINATES: protein motif:HMM:NF012907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 18 protein"
                     /protein_id="HEY5049824.1"
                     /translation="MGPELRCRPLILGLAVVMGFAGLVTTAATVAAPVAQAATPQRQV
                     SGWITYYQLANGTADAALNPDLFSDVSEFWFHAVSATSIVPSGTTPDAQLTSSVASIR
                     SHDVPVTITVTDGTGGGVMADILSNPTTRLQQEQALIAVAVRYHASGIDLDYENMAFF
                     ANGNPSLVAPTRAGFDAMVQELSGALHARGMILAVDVITKLSEPGGSPAQQVYDYPTI
                     GRYADRVRIMTYDEHAQGTGYPGGPVSGISWVDPILAFATSVIPPAKLYMGVPLYGYD
                     WSSKTNRGTAVSFTQASNLMAQYHATRQWSIPDGSPFFTYTDASGARHTVWYDDAESL
                     QARLPLVGKYGLGGIAMWSFGNEDPAIWGVLRAATYGPNPFGSVGSAVLWPGGVRVTG
                     WSIDPNSTAPINVDLYADGKFLTRTLAGVDRPDVGNIYFAYGTTHGFDTTVDLPAGQH
                     QICAYGINVGTGTTNPKLGCIGATVPTNNPTGHLETASGKAGVLTVTGWTLDPDTAAP
                     LLVDVYVDGKLTGETIANVSRPDVATAYPGWGAAHGFSTSVDVSGGTHQVCAFAINQR
                     LGTTNPSLGCSTVKVSSGSPVGDLESAQPSSGAVTVTGWTIDPNTASPIGVAVYVNGK
                     LASDGVADISRPDVAADYPPFGPEHGYSLTVDAPAGKDQVCVYGLNTGPGDGNPELGC
                     TTVTVPSGNPIGHLESVVGAKGGFTASGWALDPNTASSIEVALYVDGAFALQQTASVS
                     RPDIAADYPPFGAAHGFALPVTVKAGKHTVCAFGINVGRGDTNTSLGCEAVNTS"
     gene            complement(4260..>4525)
                     /locus_tag="VII50_02915"
     CDS             complement(4260..>4525)
                     /locus_tag="VII50_02915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5049825.1"
                     /translation="VAAGWSIDPDSNDSIPVDVYVDGKLVIRTTADAGRPDVAALYAG
                     WGPAHGFDAPLALTAGKHQICAYGINTGPGTTNPSLGCLTVHA"
BASE COUNT          746 a         1494 c         1518 g          767 t
ORIGIN      
        1 tcggcacgtt cttgcgaggc tcgaaagcgc cgaggaaggc gatgtattgc tggccacgca
       61 ggccgagtcg cgcggagata cgcgccgctt cggcctcggt cggcggcctg aaaacatcgt
      121 ggtcgacgcc gtgatacgcg acgtcgatgc gggtcgagtc ggcgtcgacg aggcggacga
      181 gctcatcgcg cgtcgccttc gacggcacga tgcacctcgt tgagcgcttc actgccgagc
      241 gaatcgccgc acggtagaac gcgccgcgcg tgggcgggta cagatcgggc tcgctgaaga
      301 acgttgcgtc gtggatcgtg gtgaccaccg ggcggcccgg cctcatgggt accgaataat
      361 tcggtgcgtg aatgaccttc gcggccacgt gttcggccac cagcgggagc ccggtttgct
      421 cccaggcgat ccgcgcggtg cggttcgtga gggacgccgg cccagctacg atctgcgcgg
      481 cgggcaccag ccgcccgtac cgctcggcgt cgacccgctg gcacaccact gcgaggtcgg
      541 cgtccttctc cgcgagtgcc gcgagcagac cgtcgacgta tctcccgagg ccaccccggt
      601 cagcgggaac agcactggcg tcgatgagaa cccgtggcgc cacgatgcag tccacctcga
      661 acggtcacgt cggtcggttg cttcggaccg tcatggtccc gggcagtagt catctgttgc
      721 tagcggcaag cttacgccgc gagcacagta gcgagccgta ggaaggatgt gggagtggtc
      781 gggtcggccg cgacagacag gtctgtcgtc gcaaccgtcg tcgtcattgc ctggaacggt
      841 gcgggcctga tcggtccttg cctcgatgcg gtcgcagggc aggagcttgc cgacggtttc
      901 gtgatctggg tgatcgacaa cgcctcaacc gactcgaccc cagccgtcgt cgaccgacat
      961 ccgatcggcg cccggctggt gaccgtcagc tccaacctcg gcttcgccgg cgccgccgag
     1021 gtcgccgtcc gtgaagcaac cacgccattc gtcgtcgtcc tgaatcaaga cgcgcgccct
     1081 gagccaggct ggctggccgc gctgctcgcg ccactgaccg gtccggatgc gcaccgcatc
     1141 gccgccgtca ccagcaaggt gatcttcacc gcggacggtc ggctgaacaa caccggcgtc
     1201 atcgtgggcc gcgacggtta tggccgcgac cgtgggtttg gtcagccgga cgacggccgg
     1261 ttcgccgagc ctgaggacgt cttcggcttc tcgggcaccg cggctgcgct acgggtggtg
     1321 gcagttcgcg acgtcggatc gttcgatcct tcgttcttcc tgtactacga ggacacggac
     1381 ctttcgtggc gcctgcagct aggtggttgg cgagtgcggt acgcgcccga cgcggtcgtg
     1441 cggcacgtgc acgccgcgtc gaccgatgtg tcatcgaagt cgttcgcctt cttcaacgag
     1501 cgcaaccggc tgctcatgtt ggcgaagtgc gcgcctcgat cccttgctgc gaaagagatt
     1561 ctgcgattcg ccggcatcac gttggtgttg ccgatccgtc gactcacccg tgtcgacatc
     1621 ccgacgggcc atcagttcag gacgccgaca cgcgtgcacg cgcttgcgtc gttcgcgcgg
     1681 atgctgccct cagctctgaa gaagaggcgc gcggtctcgc ggatctgtcg gcgaccacgc
     1741 gcagaagtag cccgcgacct cagctaaggg ctttggcgcc tttgcgcagg cccgggagcg
     1801 gcgcacgcaa cgactaggaa gtattgacgg cctcgcagcc gagtgaggtg ttcgtgtcgc
     1861 cgcgaccgac gttgatgccg aacgcacaca ccgtgtgctt gccggccttg actgtcaccg
     1921 gcaatgcgaa cccgtgcgcg gcaccgaatg gcggatagtc cgccgcaatg tcgggccgcg
     1981 acaccgaggc cgtctgctgc agcgcgaacg cgccgtcgac gtagagcgct acttcgatgg
     2041 acgacgccgt gttggggtca agtgcccagc cgctcgccgt gaatccgccc ttcgcgccaa
     2101 caaccgattc gagatggccg atcgggttgc cgctgggcac ggtgaccgtc gtacagccga
     2161 gctccgggtt gccgtcgccg ggcccggtgt tgagcccgta cacgcagacc tgatccttgc
     2221 ccgcgggcgc atcgaccgtc agcgagtacc cgtgctccgg tccgaacggc gggtagtccg
     2281 ccgcgacgtc cggccgagaa atgtcggcca caccgtcgga ggcgagcttg ccgttgacgt
     2341 aaaccgcgac gccgatcggg ctcgccgtgt tcgggtcgat ggtccagccc gtcaccgtca
     2401 ccgcacctga cgacggctgc gcgctctcga gatcgccgac cggactaccg gagctgacct
     2461 tgaccgtcga acagcccagg ctcgggtttg tggtgcccag gcgctggttg atcgcaaacg
     2521 cgcagacctg atgcgtgcca cccgacacgt cgaccgaggt cgagaagccg tgagcagcac
     2581 cccaacccgg gtaggccgta gcgacgtccg gcctcgacac gttcgcgatc gtctcgccgg
     2641 tcagcttgcc gtcgacgtag acgtccacga gcagcggcgc ggcagtgtcg gggtcgagcg
     2701 tccagccagt aaccgtcagc acgccggcct tgcccgaggc ggtctcgagg tgaccggtcg
     2761 gattgttcgt cggaacggtc gcaccgatgc agccgagctt cgggttagtt gtgccggtgc
     2821 ccacgttgat gccgtacgca cagatctggt gctggcccgc gggcaggtcg acggtggtgt
     2881 cgaagccgtg cgtcgtgccg taggcgaagt agatgttgcc gacgtccggt cgatcgacac
     2941 cggccaacgt gcgggtgagg aacttgccgt ccgcgtaaag gtcgacgttg atcggcgcgg
     3001 tcgagttcgg gtcgatcgac cagccggtca cgcgcacccc acccggccac aacaccgcgc
     3061 taccgacgct gccgaagggg tttgggccgt aggtcgctgc gcgaaggacc ccccagatcg
     3121 ccggatcctc gttgccgaac gaccacatcg cgataccacc gaggccgtac ttgccgacca
     3181 acggcaaccg cgcctgcagc gactctgcgt cgtcgtacca gaccgtgtgg cgtgcgcccg
     3241 atgcatcggt gtaggtgaag aagggtgagc cgtcgggaat cgaccattgc cgcgtcgcgt
     3301 ggtactgcgc catcaggttg gaagcctgcg taaacgacac tgccgtcccg cggttggtct
     3361 tgctcgacca gtcatagccg tacaacggca cgcccatgta caacttcgcg ggcgggatca
     3421 cactcgtagc gaacgccaga atcgggtcga cccagctgat gccactgacc ggtccgcccg
     3481 ggtagccggt gccttgcgcg tgctcgtcgt acgtcatgat ccggacccgg tcggcgtagc
     3541 gaccgatcgt cggatagtcg tagacctgct gcgccggaga tccaccgggc tcgctcagct
     3601 tcgtgatgac gtcgacggcg aggatcatcc cgcgcgcatg caacgcgcca gagagttctt
     3661 gcaccattgc atcgaaaccc gcgcgggtcg gtgcgaccaa cgacgggttg ccgttcgcga
     3721 agaacgccat gttctcgtag tcgaggtcga tgccgctcgc gtggtagcga acagcaactg
     3781 cgatcagcgc ttgctcctgt tgcaaccgcg tcgtcgggtt cgacaagatg tccgccatca
     3841 cgccaccgcc agtgccgtcg gtgaccgtga tcgtcaccgg gacgtcgtgg ctgcggatgc
     3901 tcgcgaccga cgacgtcagt tgcgcatcgg gggtcgtacc tgacggcacg atcgatgtcg
     3961 cgctcaccgc gtggaaccag aactcagaga catcggagaa caggtcgggg ttcagcgccg
     4021 catcggcggt gccgttggcg agctggtagt aggtgatcca gcccgaaacc tgccgctgtg
     4081 gagtcgccgc ctgcgccacg ggcgcggcga cggtggccgc cgtggtcacc aacccggcga
     4141 agcccatgac gaccgcaagg ccgagaatga gcggacggca gcgaagctcg ggtcccacgg
     4201 atctcctcga aaatctgacg gcacagctcg gcttgcgcag ccgacccgta acagctgggt
     4261 cacgcgtgaa cggtgaggca acctaacgac ggattcgtcg tgcctggtcc agtattgatg
     4321 ccatacgcac agatttggtg ctttccggcc gtgagagcca gcggcgcgtc gaaaccgtga
     4381 gcgggacccc agcccgcgta gagcgccgca acgtcaggcc gcccggcgtc ggcggtggtc
     4441 cggatgacca gcttcccgtc cacatagacg tctaccggga tcgaatcgtt gctatcagga
     4501 tcgatcgacc agccggcggc caccc
//
LOCUS       DATCBJ010000145         7391 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_206630, whole genome shotgun sequence.
ACCESSION   DATCBJ010000145 DATCBJ010000000
VERSION     DATCBJ010000145.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 7391)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7391)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7391
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_206630"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..463)
                     /locus_tag="VII50_02920"
     CDS             complement(<1..463)
                     /locus_tag="VII50_02920"
                     /inference="COORDINATES: protein motif:HMM:NF020348.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FABP family protein"
                     /protein_id="HEY5049826.1"
                     /translation="MTDGPLDDPAAAFETPFIPDAAVPPLHASLEPFAFLLGTWRGVG
                     VGGYPTIESFRYGQEVVFGHAGKPILTYVSRSWVLDDAGNVLRAGAGESGFWRVDAAG
                     ELEVVLAHASGLAEIWIGNLAFGRIELRTDVVVRTASAKEVTAGHRLFGTVD"
     gene            complement(685..1971)
                     /gene="ltrA"
                     /locus_tag="VII50_02925"
     CDS             complement(685..1971)
                     /gene="ltrA"
                     /locus_tag="VII50_02925"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: protein motif:HMM:TIGR04416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HEY5049827.1"
                     /translation="MRELQRKLWAAAKQSKGRRFHAVFDQICRGDVLWMAWDRVRANR
                     GAAGVDQVTLEAVEVYGVARMLEELAADLRAGVYRPAPVRRVEIPKPDGRMRPLGIPT
                     VRDRVAQQAAKLILEPLFEADFVECSFGFRPRRSATDALEKIRVGFLRGQQFVFEAGI
                     ADFFGTIDHDRLLVEVGRRVSDRRVLKLIRAWLRAGVLDGGVFTESVAGAPQGRVISP
                     LLSNIYLHAFDRAWAEHGIGELVRYADDFVVLCRSAAHAEQAHQWARVLLGDLGLVLH
                     PDKTRVVDLREGKEGFDFLGCHLRARMSGRLWEQKRIVRYYLHRWPSQRSVKRAHLRI
                     KALTGRSQVGMELEVVIGRLNLFLVGWGNYFRTGNAADKFGSMDRYVAWRLKRFLIKK
                     RGRNLRADQAGRWTRTWFHDQGLHQLMGTIRYPKAA"
     gene            complement(2646..2933)
                     /locus_tag="VII50_02930"
     CDS             complement(2646..2933)
                     /locus_tag="VII50_02930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011718900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1416 domain-containing protein"
                     /protein_id="HEY5049828.1"
                     /translation="MCGAVAGGLPVGVDVGKEAVIQGVVRRGGVPVVGYVRLLDGTGE
                     FTAEVPTSATGEFRFFAAPGTWTVRALVPGGTADQVVNAAVGEIAQVDVAV"
     gene            complement(2933..3772)
                     /locus_tag="VII50_02935"
     CDS             complement(2933..3772)
                     /locus_tag="VII50_02935"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005152658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase"
                     /protein_id="HEY5049829.1"
                     /translation="MSREAVLVDADWVEAHLSDPNIVLVEVDEDVSAYDKGHIRGAVK
                     VDWKADLQDPVRRDFIDKAGFEKLLSSRGISNDDAVILYGGNNNWFAAYAYWYFKLYG
                     HENIKLLDGGRKKWELDSRELTDEAVSRPAKSYKAKDVDTSIRAFRDDAIAAIGKLNL
                     VDVRSPDEFSGKLLAPAHLPQEQSQRGGHIPTAKNIPWSKAANDDGTFRSDDELRTLY
                     GDAGVDFGKDTIAYCRIGERSAHTWFVLHEILDQPNVKNYDGSWTEYGSLVGVPISLG
                     ADG"
     gene            complement(3769..4212)
                     /locus_tag="VII50_02940"
     CDS             complement(3769..4212)
                     /locus_tag="VII50_02940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013230720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4395 domain-containing protein"
                     /protein_id="HEY5049830.1"
                     /translation="MAGSATLVDPRGLRFAATITAIVLAVVLLTSNAIVLAVQAAVFA
                     IGAFGGLRRAPYGLLFRYTLAKRLGRPAEREPEAPPRFAQAVGLGFALVGVVGFAVGL
                     SWIGLIASAFAFIAAFLNAAFGYCLGCEMYLLSRRLLTTRKGVPA"
     gene            complement(4447..4887)
                     /locus_tag="VII50_02945"
     CDS             complement(4447..4887)
                     /locus_tag="VII50_02945"
                     /inference="COORDINATES: protein motif:HMM:NF012314.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HEY5049831.1"
                     /translation="MTWVSVIALLALVAVTTAAGLIWRSRNGRVREQADGELLTASDI
                     GAALGGTATLVQFSTVFCQPCRAARVTLGEVSAMVDGVAHVEIDAESHLDLVRRLDVM
                     RTPTVLVLDRTGRIVKRASGAPRKADVLAALGAVVPASAAPTQR"
     gene            complement(4936..5643)
                     /locus_tag="VII50_02950"
     CDS             complement(4936..5643)
                     /locus_tag="VII50_02950"
                     /inference="COORDINATES: protein motif:HMM:NF024881.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor"
                     /protein_id="HEY5049832.1"
                     /translation="MTADGCAEVRLSLGAYVLGALDPADRSRVDAHLAGCPDCRDELA
                     SFAALPGLLGRVSRSEVEAELVDPGPQLLERLLVAAAAERRHDRRRRILTSVAAGVIV
                     LAAIGVTVGVTQDHHRATPPVVTAGETFTATNPQTHVQATITEWKTGWGASVEVSVVG
                     VTADLAGPSCQLIAVGPGGKTDVAASWAAPAAGYTGLSKVTASGSTAMAAKDISAFKV
                     VGSDGKTLVWVPATWST"
     gene            complement(5640..6191)
                     /locus_tag="VII50_02955"
     CDS             complement(5640..6191)
                     /locus_tag="VII50_02955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011718897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEY5049833.1"
                     /translation="MLRQRSRGVAETEGTTDEAFIRALYDEHARPLLGYALRLTNGDR
                     HRAEDVVQETLLRAWRHPEAIHRSPDAVRPWLFTVARHIAVDAYRARRARPSEAGPDG
                     LEIVAVDDDVDRALEAWMVTDAIASLSLDHRRVLIETYYRGRSVAEAATELGVPAGTV
                     KSRTYYALRALRLILEERGVTEA"
     gene            6269..6550
                     /locus_tag="VII50_02960"
     CDS             6269..6550
                     /locus_tag="VII50_02960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5049834.1"
                     /translation="MSAGLITTLTLIYGGFSALGIAAGISILRKAGYSSWWVLTGFIP
                     VVNVVMVVAFAFADWPVLQERRRYHRLKTTEVVPDRRFSYAPAGSLADQ"
     gene            complement(6513..7163)
                     /locus_tag="VII50_02965"
     CDS             complement(6513..7163)
                     /locus_tag="VII50_02965"
                     /inference="COORDINATES: protein motif:HMM:NF018984.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CGNR zinc finger domain-containing protein"
                     /protein_id="HEY5049835.1"
                     /translation="MDSTTVTAARTDFRPDTGARWLDLVATRANWPSRGPLERLPDLD
                     TLRSWLERHALASEADPTAADLAAVRQIRAALRELGVARVEGRTPHPSAFDTVNAALA
                     RAAKAPRHIVVPDPIEPGYVRRRVPDVDAALTLIIEQAIDTMSSPDAAFLRECEETSC
                     RELFLDSSGRRRWCSTPACGIRARVRAYRARLREGAVDGGLREGATDPPRIRPARN"
BASE COUNT         1321 a         2608 c         2305 g         1157 t
ORIGIN      
        1 cgtcgaccgt cccgaagagg cggtggccgg cggtgacttc cttcgccgac gccgtccgca
       61 ccacgacatc ggtgcggagc tcaatgcggc cgaacgccag attcccgatc cagatttcgg
      121 caagaccgct ggcgtgcgcg agcacgacct cgagttcgcc tgccgcatcg actcgccaga
      181 atcccgactc cccggcgccg gcccgcaaca cgttgccggc atcgtcaagc acccagcttc
      241 ggctgacgta cgtgagtatc ggcttgcccg cgtgcccgaa gacgacttcc tggccgtagc
      301 gaaacgactc gatggtcgga tagccgccga cgccaacacc tcgccacgtg ccgagcagga
      361 aggcgaacgg ctcgagcgac gcatgcaacg gcggcaccgc ggcgtccggt atgaacggcg
      421 tttcgaacgc ggcggcgggg tcgtcgagtg gcccgtccgt caccccggcg tgcccttgct
      481 accacgaata agccggtgga cgcccagagc agccgctgcc acgatcgcgg ccgcaacaag
      541 gaccggcggc accacggtaa tcaactcggt cattggtagt ccggggcgca gtacccgcgc
      601 gagcgggcct ggttcccctt tcccctttca atccgtgcgt gcggttctcc cgcacacggc
      661 ttaccgacgg tcttctggtc ggggttacgc agccttcggg taacggatag tgcccatgag
      721 ctgatgcagg ccctggtcgt ggaaccaggt gcgggtccaa cgcccggcct gatcggcacg
      781 caggttgcga ccacgcttct tgatgaggaa gcgtttcagc cgccacgcca cgtagcgatc
      841 catcgagccg aacttgtccg ccgcgttccc ggtgcgaaag tagttgcccc atcccacaag
      901 gaacaggttc agtcgcccga tgacgacctc caactccatc cccacctggc ttcgaccggt
      961 gagcgctttg atccgcagat gtgcccgctt cacggaccgc tgcgacggcc accggtgcag
     1021 gtaatagcga acgatccgct tctgctccca caaccggccc gacatgcggg cccgaagatg
     1081 acaacccagg aaatcaaaac cctctttgcc ttcccgcaga tcgaccacac gggtcttgtc
     1141 cgggtgcagc accaacccga gatcgcccag cagcacacgt gcccactggt gagcttgctc
     1201 agcgtgggct gctgaccggc acaacaccac gaagtcgtcc gcatagcgga ccaactcccc
     1261 gatgccatgt tcggcccagg cccgatcgaa cgcgtgcaag tagatgttgg acaacagcgg
     1321 ggagataacc cggccttgtg gcgccccggc aaccgactcc gtgaacaccc caccgtccaa
     1381 cacacccgca cgcaaccatg cccggatcaa cttcagcacc cgccggtcac tcacgcgacg
     1441 cccgacctca accaacaagc ggtcatggtc gatcgtgccg aagaaatcag cgatgccggc
     1501 ctcgaagacg aactgctgtc cccgaaggaa accgaccctg atcttctcca acgcatcagt
     1561 ggcgctccgc ctcgggcgga acccgaaact gcactccacg aagtcagcct cgaacaacgg
     1621 ctcgaggatc aacttcgcgg cctgctgcgc caccctgtca cgcaccgtgg ggataccgag
     1681 tggccgcatc cggccatccg gcttcgggat ctccacccgg cgcaccggcg ccggacggta
     1741 gacacctgca cgaagatccg cagcgagctc ctccaacatg cgagcaacgc catagacctc
     1801 caccgcctcc aaggtcacct gatcgacccc agcggcacca cgattggctc ggacccgatc
     1861 ccacgccatc cacaagacgt cacccctgca gatctggtcg aacacagcgt gaaaccgccg
     1921 acccttcgac tgcttggccg cagcccatag cttgcgttgc agttctcgca cggcacccac
     1981 cggtagacgc tggtggagcg cgacaggctc acgcccgcca aagttattag accgggcggt
     2041 cccggccatt ccctcgcgct tacccactcc gctcgcataa ccaaagtcgg ggcccttccc
     2101 tcccggcgcg ttatggtgca cgccgatcag cggtactacg accccctcgg acttccgctg
     2161 ccctccctca gatttcacca ctggcttata ccgagggtct ttgcccgacg aaggccaggc
     2221 agacggatct ctcctgttcc gaaccagacc ctgatcacgt gccatctccc ataccccggg
     2281 aagacccgcc aggctgaccc cggaacaggg cccgacggac atggccttcg ccgtgacatg
     2341 agcggctcgg ctctcccact gtggatctga cgaggctgca gagttcgctt cacgctacgg
     2401 cccgtgatct tgctccctcc gaagaggctt ctgacacccc gctcagcccg ccacctctcg
     2461 acgacgaacc ggggcctgct actgggcgct ccggtaccta cccaggcggg actcacaccc
     2521 gctggcctga tccaacttgc aggacgcaac atgcggcgac cctaaacgac ttcgcatccc
     2581 tggtggcggt agaaacggcc ggttttgccg accatcacaa ccaccaggga tgcgaagtgt
     2641 ggatctcaga ccgctacgtc gacctgcgcg atctcaccga ccgcggcgtt cacgacttgg
     2701 tctgccgtgc ctcctggcac gagtgcgcga accgtccacg tgcccggagc cgcgaagaac
     2761 cggaactcac cggtcgcgga cgtcggcacc tccgcagtga actcgccggt gccgtcgagc
     2821 aatcgcacgt acccgacgac agggacgccg ccgcgtcgca cgacgccctg aatcacggct
     2881 tccttaccga cgtcgacacc gaccggcaac ccacctgcga cagctccaca catcagccat
     2941 cagcgccaag ggaaatcggg acgccgacaa gcgagccgta ttcggtccac gagccgtcgt
     3001 agttcttcac gttcggctgg tcgaggatct cgtgcagcac gaaccatgtg tgcgccgagc
     3061 gctcgccgat gcggcagtac gcgatggtgt ccttgccgaa atcgacgccg gcgtcgccgt
     3121 agagcgtgcg caactcgtcg tctgacctga aggtgccgtc gtcgtttgcg gccttgctcc
     3181 acgggatgtt cttcgcggtc gggatgtggc cgccacgctg cgactgctcc tgcggaaggt
     3241 gcgccggtgc gagcagcttg cccgagaact catcaggcga acggacgtcg accaggttga
     3301 gcttgccgat cgcggcaatc gcgtcgtcac ggaaggcccg gatggacgtg tcgacgtcct
     3361 tggccttgta cgacttcgcg ggccgagaga cggcttcgtc ggtgagctcg cggctgtcga
     3421 gctcccactt cttgcggcca ccgtcgagca gcttgatgtt ctcgtggccg tagagcttga
     3481 agtaccagta ggcgtaggcc gcgaaccagt tgttgttgcc tccgtacagg atcaccgcgt
     3541 cgtcgttgct gataccgcgc gacgacagca gcttctcgaa gcccgccttg tcgatgaagt
     3601 cgcggcggac cggatcttgc aggtcggcct tccagtcgac tttgacggcg ccacgaatgt
     3661 ggcccttgtc gtaagcagaa acgtcctcgt cgacctcgac gagcacgatg ttcgggtcgg
     3721 acaggtgggc ctcgacccaa tcggcatcca cgaggacggc ttcgcgactc atgctggaac
     3781 tcccttgcga gtagtaagga gacgacggct caacagatac atctcgcagc ccaggcagta
     3841 gccgaaggct gcgttgagaa aggcggcgat gaacgcgaac gccgaagcga tgagcccaat
     3901 ccagctcagg ccgacggcga atccgacaac gccgacaagc gcaaacccga gaccgaccgc
     3961 ttgcgcgaac cgcggcggcg cctctggttc gcgttccgcc ggccgtccga gtcgcttcgc
     4021 cagcgtgtag cggaacagca ggccgtacgg cgcccggcgc agacccccga acgccccgat
     4081 cgcgaacacc gcggcctgca cggcgagcac gatcgcgttg ctggtcaaca acacgacagc
     4141 gagaacgatt gcggtaatgg tcgcggcgaa ccgtaacccg cgtggatcta cgagagtggc
     4201 cgaaccggcc atggaagacg ctcctgacga gaacgatgga agggaaggga tctcatcgcg
     4261 tcagggccgg agtacgagcg agccggccct gtcagggcat cggacacagg caggcggcca
     4321 cgcggcacag gtcgaccgcg cgcctcttca cgagcacgac cgcatcggcc ggcacgccta
     4381 gcttcaccgt gtccacgcgt cgatggtacg gacgacggtc gcctgctgtc accttcgaac
     4441 ccgcggtcat cgctgagttg gggcagctga ggcggggact accgcgccta gggctgcgag
     4501 cacgtccgct ttgcgcggcg caccactggc ccgcttcacg atgcggcccg tgcgatcgag
     4561 caccagaacg gtgggcgtcc gcatcacgtc gaggcggcgc accaggtcga ggtgtgactc
     4621 ggcgtcgatc tcgacatgag ccacgccgtc gaccatcgca ctgacctcac caagggtcac
     4681 ccgagcggcc cgacacggct ggcagaacac cgtcgagaac tgcacaaggg tggctgtacc
     4741 gccaagtgcc gcgccaatat cggacgccgt gagcaactcg ccgtccgcct gctcacgcac
     4801 tcgcccgttg cgcgaacgcc agatcagacc ggcggcagtc gtcaccgcca ccagcgcgag
     4861 gagcgcaatc accgagaccc aggtcacagc tgcatctaac cccagggcgc cagcaggtaa
     4921 tcccgcgcgc tgcgactagg tcgaccaagt cgccggcacc cagaccaggg ttttcccgtc
     4981 ggagccgacg accttgaacg cactgatgtc cttcgccgcc atggcggtcg agccggacgc
     5041 ggtgaccttc gacagaccgg tgtacccagc ggcgggagca gcccagctgg ccgcgacgtc
     5101 ggtcttaccg cctgggccga cggcgatgag ctggcagctt gggccggcca ggtccgcggt
     5161 gacgccgacg acgctcacct cgaccgaggc cccccagccc gtcttccatt cggtgatggt
     5221 cgcttgcacg tgggtctgcg ggttggtggc ggtgaaggtc tcaccggcag tgacgacagg
     5281 cggagtcgcc cggtggtgat cttgggtcac gccaaccgtc actccgatcg cggccagcac
     5341 aatcacgccc gcggcgaccg aggtcaagat gcgccgacgc cggtcatgcc ggcgttcggc
     5401 ggcggccgca accaacaatc gctcgaggag ctgcggcccg ggatcaacca gttcggcctc
     5461 gacctcggag cggctcaccc ggccgagcag gccaggcaat gctgcaaacg atgcgagctc
     5521 gtcgcggcag tccgggcagc cggcgagatg tgcatcgacg cgactgcgat cggcagggtc
     5581 cagcgccccg agcacgtacg cgccgaggga caaccgcacc tccgcgcatc catcggcggt
     5641 catgcttcgg tcactcctct ttcttcgaga atcaagcgta gggcgcgaag tgcgtagtag
     5701 gtgcgtgact tcacggttcc ggccggcacg ccaagttcgg tggcggcctc ggcaacggat
     5761 cggccacggt agtaggtctc gatcagcacc cgacgatggt cgaggctcag actcgcgatc
     5821 gcatcggtca ccatccaggc ctccaaggcc cggtcgacgt cgtcgtcgac cgcgacaatc
     5881 tccaacccgt ccggtccggc ctccgacggc ctagcgcgcc gggctcggta ggcgtcgacc
     5941 gcgatgtgcc gggctaccgt gaaaagccat ggccggacgg cgtccgggct gcggtggatt
     6001 gcctctgggt ggcgccacgc acggagcaag gtctcctgga ccacgtcctc cgcgcggtgc
     6061 cggtcgccgt tggtgagccg cagcgcgtag ccgaggagcg gccgggcgtg ctcgtcataa
     6121 agggcacgga tgaacgcctc atccgtcgtc ccttctgttt ccgccacacc ccgtgaacgc
     6181 tggcgcagca tcaacggttc aagtccacgc tcacgatcgt cgcagctttg cgctgaagaa
     6241 acctccgcgt cgagccgatc tttcgggcat gagtgccgga ctgatcacca ccctgacgct
     6301 gatctatggc ggcttcagcg cgctcggcat cgcggccggg atctcgatcc ttcgcaaggc
     6361 cggctattcg tcctggtggg tcctgaccgg gttcatcccg gtcgtgaacg tggtcatggt
     6421 tgtggcgttc gcctttgccg actggcctgt cctgcaagag cgccggcgct accaccggct
     6481 caagaccacc gaagtcgtgc ccgaccggcg attcagttac gcgccggccg gatccttggc
     6541 ggatcagtag ccccttcgcg gagcccgcca tcaaccgcac cttcgcgcag ccgagcccgg
     6601 taggcccgca cccgcgctcg gatgccacac gcaggtgtcg aacaccaacg ccggcggccg
     6661 gacgagtcga gaaagagctc tcgacaactc gtctcctcgc actcccgcag gaaggccgcg
     6721 tcggggctgc tcatggtgtc gatggcctgc tcgatgatca acgtgagtgc ggcgtcgacg
     6781 tctgggaccc gtcgccgcac gtagccgggc tcgatcggat ccgggacgac gatgtgccgc
     6841 ggtgctttgg ccgccctggc caacgcggcg ttgacggtgt cgaaggccga cgggtgcggc
     6901 gtccggccct cgacccgagc gacgcctagc tcgcgcagcg ccgcacgaat ctggcggacg
     6961 gcggccagat cggcagccgt cgggtccgcc tcggaggcga gtgcatggcg ctcgagccag
     7021 ctgcgcagtg tgtcgagatc aggcagccgc tccagggggc cgcggctcgg ccagttcgcc
     7081 cgcgtagcga ccaggtcgag ccagcgcgcg ccggtgtcgg gacggaagtc ggtgcgagcc
     7141 gccgtcacgg tcgtcgagtc cactcctgca cggtaacgcc ccgctgcgtt acctccgggg
     7201 aagccacgct gcctgctgcc aatcgcgatg cgagcgcgaa ctggcaggat cgcgaccgtg
     7261 tcgaccctga ctgcccgcgg cgtgcgcgtc gcgttcggcg cccgcgaagt cctgcacggc
     7321 gtcgacgcga cgattacggc cggcgaccgg gtcgggctcg tgggccccaa cggcgcgggc
     7381 aagtcgacac t
//
LOCUS       DATCBJ010000146         5061 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_59782, whole genome shotgun sequence.
ACCESSION   DATCBJ010000146 DATCBJ010000000
VERSION     DATCBJ010000146.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 5061)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5061)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5061
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_59782"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..272)
                     /locus_tag="VII50_02970"
     CDS             complement(<1..272)
                     /locus_tag="VII50_02970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor WhiG"
                     /protein_id="HEY5049836.1"
                     /translation="MTARTPEPERSGVLAAAPEEDPAVAKLNAEAALRELWQEFKATG
                     ESRLRERLILHYSPLVKYVAGRVGVGLPPNIEQADLVSYGIFGLIDA"
     gene            complement(247..1128)
                     /locus_tag="VII50_02975"
                     /pseudo
     CDS             complement(247..1128)
                     /locus_tag="VII50_02975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720382.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009037 - tyrosine-based site-specific
                     recombinase activity [Evidence IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA];
                     GO_process: GO:0007049 - cell cycle [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
     gene            complement(1357..1833)
                     /locus_tag="VII50_02980"
                     /pseudo
     CDS             complement(1357..1833)
                     /locus_tag="VII50_02980"
                     /inference="COORDINATES: protein motif:HMM:NF009154.0"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
     gene            complement(1906..2214)
                     /locus_tag="VII50_02985"
     CDS             complement(1906..2214)
                     /locus_tag="VII50_02985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005056986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2469 domain-containing protein"
                     /protein_id="HEY5049837.1"
                     /translation="MSAEDLENYETDMELQLYREYRDVVGLFKYVIETERRFYLTNEY
                     VLDVRNAGGEVFFEVSMSDAWVWDMYRPARFVKNVKVVTFKDVNVEELAKSDLQVPES
                     "
     gene            complement(2211..2921)
                     /locus_tag="VII50_02990"
     CDS             complement(2211..2921)
                     /locus_tag="VII50_02990"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: protein motif:HMM:NF000598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HEY5049838.1"
                     /translation="MGNLYAFERSLAKAGLVPVAGADEAGRGACAGPLVVGAVILSNE
                     RRGQVPGLADSKLLTPAARERVYAQVVSRALAWTAVVIPAADVDRIGLHKANVEGMRR
                     AVAALGVRPAYVLTDGFAVAGFGTRGLGVWKGDRVAACIAAASVIAKVTRDRLMVAMH
                     DDFPAYGFADHKGYITPEHQGALAEHGPCAQHRRSFINVRRVDAECGRGASRVVDLGD
                     NDQVALQLSGAIPVAGLR"
     gene            complement(2932..4116)
                     /gene="lepB"
                     /locus_tag="VII50_02995"
     CDS             complement(2932..4116)
                     /gene="lepB"
                     /locus_tag="VII50_02995"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:NF021981.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HEY5049839.1"
                     /translation="MVDDPAGSDLYAPDQSMAPALSMWPGSDGDAAVERRSEPRPPGA
                     HRKTKPGKAPKPPKPPKAVKGSAAATDISPMDDTLGAVGSTGAVGLTGATTGSTVPDT
                     AAAEGVKPAKGGKAPRPFWVEAVVLLVIAAVIAVGVHSFLFQAFFIPSGSMENTLLKG
                     DRVIVNRLSYKVGHVQRGQIVVFSGVDSWTPEGSITQPHNPVLREFSKVGSYLGFAPS
                     GEQDFIKRVIGVPGDHVVCCNTQGQITVNGQALSEHYLYPGSDNRTLPFDIVVPAGRL
                     WVEGDHRNDSADSRSHTGLPGGGTIPINKVIGRAFVIVWPFSRLHRLEIPASYGALSN
                     TTASSGVSNTAVSNMTASALPSAIGVAAVLPLGAIRLRRRRRQRRRQQPCPDAGLAPN
                     TT"
     gene            complement(4161..4520)
                     /gene="rplS"
                     /locus_tag="VII50_03000"
     CDS             complement(4161..4520)
                     /gene="rplS"
                     /locus_tag="VII50_03000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009074470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HEY5049840.1"
                     /translation="MHTLDSLDAASLRSDVPDFRPGDTLKVHVRVVEGTRSRVQVFQG
                     VVIRRSGSGVRETFTVRKVSFGVGVERTFPVHTPVLDKIEIVTRGDVRRAKLYYLREL
                     RGKKAKIREKRDTTPAR"
     gene            complement(4617..>5061)
                     /locus_tag="VII50_03005"
     CDS             complement(4617..>5061)
                     /locus_tag="VII50_03005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HEY5049841.1"
                     /translation="ACGRYEGIDYRVVDDARRRMRVDELSIGDYVLGGGEAAALVVLE
                     AVVRLLPGVVGNAESLVEESHVAGLLEGPAYTKPPSWRGLDVPAVLTSGNHAEIARWR
                     RDQALRRTVERRPDLVAALEADTVSETDARVIEETIRSHRGPVAD"
BASE COUNT          838 a         1750 c         1660 g          813 t
ORIGIN      
        1 gcgtcgatca atccgaagat gccgtacgac accagatcgg cctgctcgat gttgggcggc
       61 aggccgacgc cgacgcggcc ggccacgtat ttcaccagcg gtgagtaatg caagatgagc
      121 cgctcgcgaa gacggctctc gccggtggcc ttgaactcct gccacagttc gcgcagcgcc
      181 gcctcggcgt tcagcttcgc aacggcgggg tcttcttcgg gtgctgcggc tagaacgccc
      241 gagcgctcag gctctggggt gcgcgcggtc atatgtagcc ctcagtcgct cgacggaaac
      301 gtgcgtgtaa acctgtgtcg ttgcgagcgt agcgtgacca agtatctcct ggacggttct
      361 taggtcagcg ccaccctcaa gaaggtgcgt cgccgcactg tgccgaagac catgcggacc
      421 gatgtcgggg gcgtcgggaa ccagcgcgat ccgggcgtgc accattcgcc ggacagcgcg
      481 ctggtcgagg cggcgacccc gcgcacccag gaacagcgcc gcgccgctgt cggcggtcac
      541 cagggccggc cggcccgacc gctgccaacg ttcaacagcc ctcacggccg ggacgccgag
      601 gggaaccgtc cgctgcttgt tgcccttccc gagcaccctg acgacatgcc gggcctgatc
      661 gatgtcatcg acgtcgagcc cgaccagttc gccgacccgg atgccggtgg catacagcag
      721 ttcgagtacc gcgtcgtcgc gcagctgttc tcgactgacc tggccaggcg ggccctcagc
      781 tggaggttcc agcagtgcga ccgcttgggc ctgcgacagc acgcccggca ggagccgctg
      841 ggccttcggc gtggccagga tcagaccggg gtcgggcgtc agccagccgc gccgtgcgca
      901 gaaggcggta aagactcgag cggacgccgc gtgcctggcg atcgtcgtcc tcgccttgcc
      961 tgtggtcgtc agtttggcca gccagctgcg caagaccccg atgtcgagac cagacacgtg
     1021 cgggcggccc atccggtctg catggttgag caaactcccg atgtcagcgg tgtatgcccg
     1081 gatcgtgtgc tcagacagcc cacgctcaag gcgcaggtgc ctgacaaagg ctgcgagaac
     1141 ctccgcagcc tcgggtgcct cgggtgcctc ggagacgcct ggcgcgtcgc gtgcatcgag
     1201 cgccggtccg gatgccacca tgagcgcggc ccgttcatcg actcccagct gcgctcccat
     1261 ctctgcaccg tcgcgggcaa gtgtcgcggg gtcaagcacc gacagccgga cgcgccggaa
     1321 ggtagcgcag caaggctcgg cgaggtgccc gaccgaagtc atgtcggaca gcggccgcaa
     1381 gaccgacacg acgtcgacgc gcacctcggt atagcgagcg aactcgcgcg tgctcgatgc
     1441 cacggcacct gcacccaaaa tgtggtcgcc ggcccgctgg tcggcgagcc agcgcaacgc
     1501 gagtcggcgc agccgggcgg cctgcgctgg cgtcaccgcg tcgagcggcg tgccgaaccc
     1561 gacgccgcgg cgggtcttga cctcgcacat caccagcacg tcgccgtcga gcgcgacgat
     1621 gtcgacctcg ccttcacgac accgccagtt gcgggccacg atcgtcaggc ctgcggcgag
     1681 catgtgcgcg gcggcaacct cttcgccgta gcgaccaagc ccgccacgat cgccagtaga
     1741 cgcaactgcg ctggcctcgt cggaactgcg aaccacgccc agcaccgcca gtaggcactg
     1801 tggtggcgaa cgcctgccgg cagtcccacg caccgacctc tgtccatggg cgcgaacgtg
     1861 aagagagggc gtggacaacc cacgagtggc gtcaggttgc cgttcctagg actctggtac
     1921 ctgcagatcg ctcttcgcga gctcttcgac gttgacgtcc ttgaacgtca caaccttgac
     1981 gttcttcacg aaccgggccg gtcggtacat gtcccacacc caggcatcgg acatgctcac
     2041 ctcgaaaaac acctcgccgc ccgcgttgcg gacgtcgagc acgtattcgt tggtgaggta
     2101 aaaccggcgc tcggtctcga tgacgtattt gaaaaggccc acgacgtcgc ggtactcccg
     2161 atagagctga agctccatat cggtctcgta gttctcaagg tcttccgcgc tcatcgaagc
     2221 cccgcaaccg gaatcgcgcc cgacagttgg agtgccacct gatcattgtc gcccagatcg
     2281 acgacgcgtg acgccccgcg cccacattcg gcgtccaccc gtctgacgtt gatgaaagac
     2341 cgccgatgct gcgcgcaagg cccgtgctcg gcgagcgccc cctggtgctc cggggtgatg
     2401 tagcccttgt ggtcggcgaa tccgtaggcc ggaaaatcat cgtgcatagc gaccataagg
     2461 cggtcccgag tcaccttggc gatcaccgac gccgccgcga tgcacgcggc gactcggtcg
     2521 cctttccaca cgccgagccc gcgcgttccg aagccggcga ccgcgaaccc gtccgtgagg
     2581 acgtaggccg gtcggactcc aagggcggcc accgcgcggc gcatgccttc gacgttcgcc
     2641 ttgtgcaatc cgatgcgatc gacgtcggcc gcagggatca ccaccgcggt ccaggccagc
     2701 gccctcgaaa cgacctgcgc gtacacccgc tcccgagcgg caggcgtgag cagcttggag
     2761 tccgcgagac ccggcacctg gccgcggcgc tcgttcgaga ggatcaccgc acccacgacg
     2821 agcgggccgg cgcatgcccc gcgacccgcc tcgtcagcgc ccgcgaccgg cacgaggccg
     2881 gccttggcga ggctgcgctc gaaggcatac aggttcccca tcgcaggaac cctacgtcgt
     2941 gttcggcgcc aaaccggcat ctgggcatgg ttgttggcgc cgtcgctgtc gacgtcggcg
     3001 tcgcagccgt atcgcaccca gtggcagcac cgcagccacg ccgatcgccg atggcagggc
     3061 agacgcagtc atgttcgaca ccgcggtgtt cgacaccccg gatgatgcgg tcgtgttcga
     3121 cagggcgccg taggacgccg ggatctccag gcgatgaagt cgcgagaacg gccacacgat
     3181 cacgaatgcc cgcccgatga ccttgttgat cgggatcgtg ccgccaccag gcaatccggt
     3241 gtgcgaacgt gaatccgcgg agtcgttgcg atggtcgcct tcgacccaga gtcggcccgc
     3301 gggcacgacg atgtcgaaag gcaacgtccg gttgtcgctg cccggataca gatagtgctc
     3361 cgagagcgct tggccgttga cggtgatctg gccctgggtg ttgcagcaga cgacgtggtc
     3421 gcccggtacg ccgatgacgc gcttgatgaa gtcttgttcg ccggacggcg cgaagccgag
     3481 gtagctgccg accttgctga actcgcgcaa taccgggttg tgtggttgcg tgatgctgcc
     3541 ttccggcgtc cacgagtcga cgccactgaa cacgacgatc tggccgcgct ggacgtgccc
     3601 caccttgtag gacagccggt tgacgatgac ccggtcgccc ttcagcagcg tgttctccat
     3661 cgaccccgag ggaatgaaga acgcctggaa caagaagctg tggacgccga cggcgatgac
     3721 cgcggcgata accagcagga cgacggcctc gacccagaac ggtcgaggtg ccttcccacc
     3781 cttggccggc ttgacgccct ccgccgctgc ggtgtcggga accgtggatc ccgtcgtcgc
     3841 gcccgtcaat ccaactgcgc cggtcgatcc caccgcgccg agagtgtcgt ccataggcga
     3901 aatgtcggtc gctgcggcgc tgcccttaac tgccttaggc ggcttcggcg gcttcggtgc
     3961 cttgccaggc ttggtcttgc ggtgcgcgcc aggcggccgt ggctcggagc gccgctcaac
     4021 agcggcatcg ccgtcggagc ccggccacat cgacagcgct ggcgccatcg actggtcagg
     4081 cgcgtagagg tcgcttccgg ccggatcgtc gaccacgtga tctaggccgc tcgtcgtgat
     4141 gtcgccggcg tcgcgagacg tcagcgggcc ggggtcgtgt cgcgcttctc gcggatcttg
     4201 gccttcttgc cgcgcagctc gcgcaggtag tacagcttcg ctcggcggac gtcgccccga
     4261 gtcacgatct cgatcttgtc gaggactggg gtgtgcaccg gaaaggtgcg ctcgacaccc
     4321 accccgaagc tgaccttgcg gactgtgaag gtctcgcgca cgccgctgcc cgaacgtcgg
     4381 atcaccacgc cctggaagac ctggacacgc gatcgggtgc cttcaacgac ccggacgtgc
     4441 accttcaggg tgtcgccagg ccggaagtcg gggacgtccg agcgcaatga ggcggcgtcg
     4501 agtgagtcca aggtgtgcat ggcgttgtcg tcctgcctga tgggggccgg tcagtggtgt
     4561 gcgtgcccgg ccggggcgtg acggtgctca aaacgagccg gcctcgggcg accgccctag
     4621 tctgccacag gtcctcgatg cgaccgaatc gtctcctcga tcaccctcgc gtccgtctcg
     4681 gaaaccgtgt ccgcttcgag ggcggcaacc aggtcagggc gccgctcgac ggtccgccgc
     4741 agtgcctggt ctcgccgcca gcgtgcgatc tcagcatggt tgcccgacgt gaggactgct
     4801 ggcacatcga ggccgcgcca gctcggtggc ttcgtatagg ccggaccctc gagcagccca
     4861 gcgacgtgcg actcctcgac gagagattcg gcgttgccaa ccaccccggg caagagccga
     4921 accaccgctt ccaggacgac gagggcagcc gcctctcctc cgcccaagac gtagtccccg
     4981 atcgaaagct cgtcgacccg catccgtcgc cgcgcatcgt cgaccacccg gtagtcgatg
     5041 ccctcatagc ggccacacgc g
//
LOCUS       DATCBJ010000147         5355 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_279743, whole genome shotgun sequence.
ACCESSION   DATCBJ010000147 DATCBJ010000000
VERSION     DATCBJ010000147.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 5355)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5355)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5355
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_279743"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1007
                     /locus_tag="VII50_03010"
     CDS             <1..1007
                     /locus_tag="VII50_03010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEY5049842.1"
                     /translation="HLLGLRASLAASGHTCPPVHLKLLIEGEEESGSPAFADLLREKR
                     DRLGCDVIVVSDNGLYAAGVITTCVRMRGLTDCQIDLHGPDVDLHSGSFGGSVPNPLT
                     VMTHLLAGLHDEFNHVTLDGFYDDVAPLTDIERDLIARLPFDDAQWLETAESRATFGE
                     AGYSTLERIWARPTCEIHGVWGGYIGPGHKTIVPTDAHAKVSFRLVANQRPVDVQTAL
                     RAYITANVPNGITANVEFFGDGVRPYLVPIDDPTVQALVRALGRAHDDEVLFTGEGGS
                     GPEADIAEILQAPLIFLGAGLPTDRVHAPDEHAVISVLLKGAETAAYLWDELAALPSL
                     "
     gene            1058..1915
                     /gene="nudC"
                     /locus_tag="VII50_03015"
     CDS             1058..1915
                     /gene="nudC"
                     /locus_tag="VII50_03015"
                     /EC_number="3.6.1.22"
                     /inference="COORDINATES: protein motif:HMM:NF001299.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000210 - NAD+ diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) diphosphatase"
                     /protein_id="HEY5049843.1"
                     /translation="MRADDEWLARAWANPSSRVIVVDGGRALVDDHDALVALGPADSP
                     AGERYFLGIAAPTDAGDAVGVFAVAAPLPDPSPSGMRAANLREVGANLSDADAGLFAH
                     AAALEQWHARHVRCPLCGAATVVALAGHIRRCVNDGSEHYPRTDPAVIMLVTDSDDRC
                     LLGRQPSWPAGRMSTLAGYVEAGETLERAVVREVFEEVGVRVGDVRYVASQPWPFPSS
                     LMLAFTAKATTTELTIDGDEIAEARWFTREELRRAITGRSLRLPMSASVAFYLIDGWF
                     GGDLARLAH"
     gene            complement(1932..2201)
                     /locus_tag="VII50_03020"
     CDS             complement(1932..2201)
                     /locus_tag="VII50_03020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mycoredoxin"
                     /protein_id="HEY5049844.1"
                     /translation="MTASAEVRTPLVIYSTTWCGYCRTLKRQLADEGIAFTEVNIEET
                     PGAAEFVMGINRGDQTVPTVLFPDGSTLTNPRLSAVLAQLGAQDS"
     gene            2349..4520
                     /locus_tag="VII50_03025"
     CDS             2349..4520
                     /locus_tag="VII50_03025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009073258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase UvrD2"
                     /protein_id="HEY5049845.1"
                     /translation="MTAVAEPEILSALDPEQRSAAEAVRGPVCILAGAGTGKTRAITH
                     RIAFAVKTGTVSPAHVLAVTFTARAAGELRGRLRGLGAGAVQARTFHSAALRQLQYFW
                     PRTIGGELPAILKSKVPLLAEVAGRLRLGLSSSDLRDVASEIEWMKALQRPPDDYASS
                     VLLAHRIAPRAVTEISALYAGYEDLKVRKNQFDFEDLLLHATALLETEPAVAAQVRDQ
                     YRYFVVDEYQDVNPAQQRLLDAWLGGRDDLCVVGDPEQTIYSFAGASPDYLTGFSTRY
                     PKATVVRLVRDYRSTPQVVALANRLSGGALVAQLPVGPVPAVDSYSDEVAEAAAVATK
                     ISRLIADGTPAREIAVLFRINAQSETFEQALAEAGVPYVLRGGERFFERPEVREAVFL
                     LRGAARAEQPDEGTLADAVRGVLAAVGFGPVPPSGSGVARDRWESLAALVRLAEERVG
                     GDSAVAEFAAAPDGAEAAVSDEAITVPEGREGGSALADFVADLEERASLQHAPVVDGV
                     TLASLHAAKGLEWDAVFLVGLVDGTLPIVYAETAAQVDEERRLLYVGITRARRHLALS
                     WAAARSPGGRATRRPSRFLDNVREATTGGSVQPGHARSPLAAKSVRDRVERCRVCQKL
                     LTSGAARKLGRCTDCPGSVDEELFERLRSWRLERSRELKQPAFCVFTDATLTRIAEVR
                     PQTAQELATIGGVGPAKLTAYAADVLEMCSTSSSVDWIRLP"
     gene            4697..4936
                     /locus_tag="VII50_03030"
     CDS             4697..4936
                     /locus_tag="VII50_03030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5049846.1"
                     /translation="MSGLEGSGTTAAAMWRPMTAAVNAAAVNTATVTTAQHQTVAPTQ
                     SLKPAALHTQIKQYVVTAPDAGRGNKGTSPWGFPV"
     gene            5141..>5355
                     /locus_tag="VII50_03035"
     CDS             5141..>5355
                     /locus_tag="VII50_03035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019511206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WhiB family transcriptional regulator"
                     /protein_id="HEY5049847.1"
                     /translation="MLTGPFDRVLAQALRRSLEVPCLTVDPELFFAESPEDVETAKAM
                     CAECPVRTACLTAALDRQEPWGVWGAE"
BASE COUNT          843 a         1787 c         1820 g          905 t
ORIGIN      
        1 accacttgct tgggttgcgg gcgagtctcg cggcgagcgg gcacacttgc ccgccggtgc
       61 atctgaagct gctgatcgaa ggcgaagagg aatccgggtc gccggcgttc gccgacctgc
      121 ttcgcgagaa gcgcgatcgc ctcggctgcg atgtgatcgt cgtgagcgac aacggcctct
      181 acgccgctgg tgtcatcacc acctgtgtgc gcatgcgcgg cctgaccgat tgccagattg
      241 acctgcacgg gccagacgtc gatttgcatt cgggctcgtt cggcggatcg gtgccgaacc
      301 ccttgaccgt gatgacgcac ctgctcgccg gactgcacga tgagttcaac cacgtcacgc
      361 tggacggctt ctacgacgac gttgcgccgc tgaccgacat cgagcgcgac ctaattgcgc
      421 gactgccgtt tgacgacgcg cagtggctcg agacggctga gagtcgcgca acattcggcg
      481 aggccggcta cagcacgctc gagcgcatct gggcgagacc gacgtgtgag attcacggag
      541 tctggggtgg ctacataggc cccggccaca agaccatcgt gccgaccgat gcgcacgcca
      601 aggtgtcgtt tcggctcgtg gcgaatcagc gaccagtcga cgtccagact gcgctacgcg
      661 cgtacatcac agccaacgtt cctaacggca tcaccgcgaa cgttgagttc ttcggcgacg
      721 gcgtgcggcc ctatctggtg ccgatcgacg acccgaccgt gcaagcgttg gtgcgcgcac
      781 ttggccgggc gcacgacgac gaggtgctgt tcaccggcga aggtggatcc ggacccgagg
      841 ccgatatcgc tgagattctc caagccccgc tgatattcct gggtgccggc ctcccgaccg
      901 accgcgtcca cgcacccgat gagcacgcgg tcatttcggt gctactcaaa ggcgccgaaa
      961 cagcggccta cttgtgggac gaactcgccg cgctgccttc gctatgaccc ggcagccacc
     1021 gccgttgggc cgttccggca tcgatcgagc agcacatttg agggcggacg acgagtggct
     1081 ggcgcgcgcg tgggccaacc cctcgtcccg cgtgattgtc gtcgacgggg gtcgtgcgct
     1141 cgtcgatgac cacgacgcgc tcgtggcttt ggggccggcc gattcgccag cgggcgagcg
     1201 atacttcctc ggcatagccg caccaacgga tgccggcgac gccgttgggg tgttcgccgt
     1261 cgcggcgcca ttgcccgacc catcgccgtc cggcatgaga gcggcaaact tgcgcgaggt
     1321 cggcgcgaac ctgtcggacg ccgacgcggg cctcttcgcg cacgcggcgg cgctggagca
     1381 gtggcacgca cgacatgtgc gctgtccgtt gtgcggtgcg gcgacggtcg tggcgcttgc
     1441 cgggcacatc cggcgttgcg tcaatgacgg cagcgagcac tatccgcgta ccgacccggc
     1501 cgtcatcatg ctggtgaccg acagcgacga ccgatgcctg ctcggtcggc agccgagttg
     1561 gccagccggc cggatgtcca ccctggctgg atatgtcgag gcgggcgaga cgctcgagcg
     1621 cgccgtcgtc cgcgaagtgt tcgaggaagt tggcgtcagg gtcggcgacg tgcgctacgt
     1681 cgcaagtcag ccctggccgt ttccgtcgag cttgatgctg gcgttcacgg cgaaggccac
     1741 gaccacagag ctgacgatcg acggagacga gattgccgaa gcgcggtggt tcacccgcga
     1801 agagttgcgc agggcgatca ccggccgctc gttacgactg ccgatgagcg cgtcggtcgc
     1861 cttctacctc atcgacgggt ggtttggcgg cgacctcgcg cgcctcgcgc actaatggtc
     1921 ctactggccg ctcaggagtc ttgggcaccg agttgcgcga ggacggcgga cagccgcggg
     1981 ttggtcagcg tgcttccgtc gggaaagagc acggtcggca cggtttggtc gccgcggttg
     2041 atccccatca cgaactccgc agcgcccggg gtctcctcga tattgacctc ggtgaacgcg
     2101 attccctcgt ccgcgagctg gcgcttcagg gtgcggcagt acccacacca cgtcgtgctg
     2161 tagatcacca gcggggtgcg aacctcggct gaggcggtca cggtagaact cctcggattg
     2221 ggtcggtgcg gctcgtgcct tcaaatcatc acaaccgtcg accacccgcg gttcttcctg
     2281 caggcgcttc ctcacagcgg tggcctgtgc tgcgacgcgg tgtcggccgg ccctgccaag
     2341 atggcccggt gaccgcggta gctgagcctg agatcttgag cgcgctcgat cccgagcagc
     2401 ggtccgcggc agaggccgtt cgggggccgg tctgcatcct ggccggcgcg ggtaccggca
     2461 agaccagggc gatcacccac cggatcgctt tcgcggtcaa gaccggcacg gtgtctccgg
     2521 cgcatgtgct cgcggtgacc ttcacggcga gagcggccgg cgaactgcgc ggtcggttgc
     2581 gtgggctcgg cgccggcgcc gtccaagcgc gaacctttca ctccgccgca ctgcggcagc
     2641 tgcagtactt ctggccgcgg accattggcg gcgagctacc ggcgatcctg aagtcgaagg
     2701 tgccgctgct cgccgaggta gccgggcgat tgcgtctcgg gctttcatcg tctgacctgc
     2761 gtgacgtcgc ctccgagatc gagtggatga aggcgctcca gcgaccgccc gacgactacg
     2821 cgtcgtccgt gctgctcgcg catcgcatcg ccccgcgggc ggtcaccgag atcagcgcgc
     2881 tctacgccgg ctacgaagac ctcaaagtgc gcaagaacca gttcgatttc gaggatctgc
     2941 tgctgcacgc cacggcactt ctcgaaaccg agccggcggt agcagcccag gtccgtgacc
     3001 agtaccgcta cttcgtggtc gacgagtacc aggacgtcaa cccggctcag cagcggttgc
     3061 tcgacgcgtg gctcggcggc cgcgacgacc tgtgcgtcgt gggcgatccg gagcagacga
     3121 tctactcgtt cgccggtgcg tcgcccgact acctcactgg attctcgacg cgatatccga
     3181 aagcgaccgt cgtccggctg gtgcgtgact accgatcgac gccgcaggtc gtcgcactgg
     3241 ccaaccggct gtcgggcggt gcgctcgtcg cccaactgcc cgtcgggccg gttcccgccg
     3301 tcgacagtta ctccgacgag gtcgccgagg cggccgccgt cgcgaccaag atttcccggc
     3361 tgatcgccga cggcacaccc gcgcgtgaga tcgcggtgct gttccgcatc aacgcgcagt
     3421 ccgagacctt cgagcaggcg ctcgctgaag cgggggtgcc ctacgtgctg cggggcggtg
     3481 agcggttctt cgagcgtccg gaagtccgtg aagccgtgtt cttgctacgc ggcgcggcgc
     3541 gggcggagca gcccgacgag ggcacgttgg ctgacgctgt gcgtggcgtc ctcgctgcag
     3601 tgggattcgg cccggtgccg ccgtccggat cgggcgtggc acgtgatcgg tgggagtcct
     3661 tggccgcact cgttcggctt gcggaagagc gcgtcggcgg cgactctgcg gtcgccgagt
     3721 tcgccgctgc gccggacggc gcggaagcgg cggtgtctga cgaagcgatc acggtgccgg
     3781 aaggccgcga gggtgggtcg gccctggcgg atttcgtcgc cgacctggag gaacgcgcgt
     3841 ccttgcagca cgcgcccgtc gtcgacggcg tcacgctggc ctcgctgcac gccgccaagg
     3901 ggcttgagtg ggacgccgtc ttcctggtcg gcctggtcga cggcacgctg ccgatcgtct
     3961 acgcagagac ggcggcacaa gtcgacgagg aacgccggtt gctctacgtc ggcattacca
     4021 gggcccgacg ccatctcgcc ctctcgtggg cggcggcacg gtctcccggc ggccgcgcca
     4081 cccggcgtcc gagccgtttc ctcgacaacg tgcgcgaggc cacgaccggc gggtcggtgc
     4141 agccggggca cgcgcggtcg cccttagccg ctaagtcagt ccgcgaccgc gtcgagcggt
     4201 gcagggtctg tcaaaagctt ttgaccagtg gcgccgcgcg caagctcggg cgttgcaccg
     4261 actgtccggg cagcgttgac gaggagttgt tcgaacgctt gcgctcgtgg cggctcgagc
     4321 gatcgaggga actgaagcag cccgcattct gcgtgttcac cgacgcgacg ctgacccgga
     4381 tcgccgaggt gcgcccgcag acggcgcagg agctcgcgac gatcggcggc gtcggtcccg
     4441 ccaagctcac cgcgtacgca gccgacgtgc tcgagatgtg cagcacgtcg agttccgtcg
     4501 actggattcg gctcccctga ctgccgcaca taaatgccca ataaatacgt tgcccgcttc
     4561 ccgccgttgg ccgtagcctg cccactggta actgttgatg agggcacgcc cgagagggcg
     4621 aagccccgag aacggaaggt ggtgagccgc agtgcggacc gtaactaagg cagtggcgtt
     4681 cgctcctgag ttcgccatgt ccgggctcga gggcagcggc acgaccgctg ccgctatgtg
     4741 gcgtccgatg accgcagcag tcaatgcagc agcagtcaat acggcaacag tcactacggc
     4801 acagcaccag accgtcgcac ctacgcagag cctcaagccc gcagcgctcc acacccagat
     4861 caagcagtac gtggtcacgg cgccggatgc cggtcgtggc aacaagggaa cctcgccttg
     4921 ggggttcccg gtctgagaca cctcagaccg gctctccaag gccgcggaac cctcaccggg
     4981 atccgcggcc tttttgttgt ccggtttgct atccagcccg ttatccactc gctcgtccac
     5041 cgctcaatcc ggcgccaccg aaacgtcgta cctcacaact agaaacctca gacaacgaga
     5101 aacctcagac aacgagaaac ctccaggagg ggtgacgcag atgctgaccg gcccgtttga
     5161 ccgcgtgctt gcccaggcgc tgcgacggtc gcttgaagtc ccgtgtctca cggtcgaccc
     5221 ggagctgttc ttcgccgagt cgcccgagga cgtcgagacc gccaaggcga tgtgcgcgga
     5281 gtgccctgtg cgtaccgcgt gcctgacggc ggcgctcgac cgccaggagc catggggcgt
     5341 ctggggcgcc gagtt
//
LOCUS       DATCBJ010000148         2265 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_463897, whole genome shotgun sequence.
ACCESSION   DATCBJ010000148 DATCBJ010000000
VERSION     DATCBJ010000148.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2265)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2265)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2265
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_463897"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..762
                     /locus_tag="VII50_03040"
     CDS             <1..762
                     /locus_tag="VII50_03040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5049848.1"
                     /translation="VGSSLTAGGEEHATAWGATSGIVDLGTIGGGTYSAALALNDRGQ
                     AVGYSQLADGYEHAALWQVSSQPDEQAITGTIPAGVLTISTPYTPTAPLDLGTLSLDP
                     SGTYFTGSAPFGVAPSAASEILVTDTRAGDLPWTAQAQASALTDGGSNPRSTINGENV
                     GLTNLTEVPVAGNGFNGLASNFTTFANPAAAPPVSPTDTNLAGLGNAAHDIAQAHQGF
                     GSIGLTGLLTLNAPSSTEAGLFKGTITFTVVGGLQ"
     gene            complement(1102..1314)
                     /locus_tag="VII50_03045"
     CDS             complement(1102..1314)
                     /locus_tag="VII50_03045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5049849.1"
                     /translation="MLDHTPPAVDPPCDVFSHFTSPPPAGATCPPLIDTLVVVAFHGD
                     GDSGCTHETGQNGVHVAQGWLGARHN"
     gene            complement(1683..1913)
                     /locus_tag="VII50_03050"
     CDS             complement(1683..1913)
                     /locus_tag="VII50_03050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5049850.1"
                     /translation="MESSSTNNRFNEVLAATRSHAPEFMGLSDLQARALAEKLGIELQ
                     VRSKDEPITADARARRLTVDLSIGVVTDARAG"
BASE COUNT          390 a          743 c          748 g          384 t
ORIGIN      
        1 gtcgggtcga gcctgacagc gggcggcgag gaacatgcca ccgcctgggg cgcaacgtcc
       61 ggcatcgttg acctgggcac gatcggtggc ggcacctaca gcgccgcact cgccctcaac
      121 gatcgcggcc aagccgtcgg ctacagccaa ctcgcggacg gatacgagca cgcggcactg
      181 tggcaggtct catcccaacc cgacgagcaa gccatcaccg gcaccatccc agcaggcgtg
      241 ctgacgatca gcacccccta cacaccgacc gctcccctcg atctcggcac actctccttg
      301 gacccaagcg gcacatactt caccggtagc gcaccgttcg gcgttgcccc gagtgccgcg
      361 agcgaaatcc tcgtcaccga cacccgcgcc ggtgacctac cctggaccgc tcaggcgcaa
      421 gcctcggcac tgaccgacgg cggctcgaac cccagatcca ccatcaacgg tgagaacgtt
      481 ggcctgacca acctcactga ggtcccggtc gccggcaacg gcttcaacgg cttggcgagc
      541 aacttcacca cgttcgccaa cccggcagcg gcaccgccgg tttccccgac cgacaccaac
      601 ttggctggtc tcggtaacgc agcccatgac atcgcgcagg cgcatcaggg cttcggttcc
      661 atcggtctga ccggcttgct gaccctgaac gctccgtcct ccaccgaggc tggtttgttc
      721 aagggcacga tcaccttcac ggtcgtgggc ggcttgcagt aggagcaccg aagccgtgtt
      781 cggaaacgtc gcgtggctgg acatcttgca aggatcagac gtacgcgcgc gcccgtgtgg
      841 tgggaccgga tgcgcaccgc gacacaagag ctcctctcag ccacggttgc aacgtgcgag
      901 gtgaggactg ccgccggccg atgtcggtcg tcgaggtgga agatgcgtcg gcgctcggca
      961 agcgactgcg gtcgctgcgt gaagccgccg gcttgagtca gggccacttt ggggtcacta
     1021 gccgaattgg gcgagcgcca tgtgcggcga ctcgaacgcg gtgagcggcg gactcgcgtc
     1081 tcgactcttg agaggatcgc gttagttgtg acgcgcgccc agccaaccct gggcgacgtg
     1141 gacgccgttc tgaccggtct cgtgagtgca gccggaatcg ccatcgccgt ggaaagcgac
     1201 taccacgagc gtgtcgatca acggcggaca cgtcgcaccc gccggcggcg gcgacgtgaa
     1261 gtgactgaac acgtcacaag gtgggtcgac agcgggtggg gtgtggtcga gcaccattcc
     1321 cattcgtggc ctgacggtgc gcggcgtgtg aggcgaagga gcttcacgcg catcgcggtc
     1381 acggaaacta cagcttcctc gatggatacg gacgccagct tctaggtgag cggcgttgcg
     1441 ctgtcgcggg cgccttcagt tgggcgtcta cagcttgcag cggtggctgg cctgcgtgcc
     1501 gttggctcgg cgaagcgacg cggaccaagt gatcggtggc cctcccgaga actcgctgcg
     1561 gcaaccgctg gggttgttgg cgcaccgggg cgctcgcgaa cgccatcatt tgccgacgac
     1621 atgatcgttc tcctcgcgat tcggttcgct ggaggctggg cgacggtcag gcgcgcaggc
     1681 cgctacccgg ctcgggcatc agtcaccaca ccgatgctca gatcgacggt caggcgtcga
     1741 gcacgcgcgt ccgcggtgat cggctcgtcc ttggaccgga cctgcagctc aataccgagc
     1801 ttctcggcca aggcccgcgc ctgcaagtcg gacaacccca tgaactccgg tgcatgcgag
     1861 cgcgtcgcgg ctagcacttc gttgaatctg ttgttagtgc tggatgactc cataccaaag
     1921 agtaggcata gctctaccgc cgcggggaaa cgcagcgacc gcagcgtggc cggccccgca
     1981 ggcaggaaaa cgtccgctac tgccgcaatg acgagcgctt cgtcgtgccg aatgtcgtac
     2041 gctcgccggg tgcatggagc ctcttgatgg accgagtgct cgcgactgtc gccgtgttgc
     2101 gccgctaggg ttttagggtc gtgcacggct tgccgacgaa caaggtgtga aggcttcgcg
     2161 tgtgccggcg gggtcagtgg gctgtgtggc cttgctccgc cgtgaggcgc ggtctttggt
     2221 gcgatggtca tgacacgggc cgttcgcaaa gccggccagc acgag
//
LOCUS       DATCBJ010000149         2269 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_463782, whole genome shotgun sequence.
ACCESSION   DATCBJ010000149 DATCBJ010000000
VERSION     DATCBJ010000149.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2269)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2269)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2269
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_463782"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1007
                     /locus_tag="VII50_03055"
     CDS             <1..1007
                     /locus_tag="VII50_03055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009085825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEY5049851.1"
                     /translation="FTSSGTEANEAALLLATQYRRSNQVLALRNSYHGRSFASIAITG
                     NRGWSASSLSPVNVSYVQSGYRYRSPFGHLSDADFIAACADDLRDVITTTTSGDVACM
                     IAEPIQGVGGFATPPDGMFAAFKEILDSYGILFISDEVQTGWGRTGEHFWGIQAHGVV
                     PDAMTFAKGLGNGTSIGGVVARAEIMDCLNANSISTFGGNPLVTAAANATLDYVLSHD
                     LQANAAKVGTALLEGIRGLQHTYPFVGDVRGKGLMIGVELVVPGSKTPDPGAATRMME
                     ETRERGLLIGKGGLHGNVLRIAPALTVTEAEAAEGLDVLAKSFAAVESANIPAAAGAR
                     "
     gene            1004..>2269
                     /gene="mmsA"
                     /locus_tag="VII50_03060"
     CDS             1004..>2269
                     /gene="mmsA"
                     /locus_tag="VII50_03060"
                     /EC_number="1.2.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009082728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004491 - methylmalonate-semialdehyde
                     dehydrogenase (acylating, NAD) activity [Evidence IEA];
                     GO_process: GO:0006574 - valine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-acylating methylmalonate-semialdehyde
                     dehydrogenase"
                     /protein_id="HEY5049852.1"
                     /translation="MSEIRRITHWIGGKPHTGESARHGDVFDPATGQVTGHVDFASSE
                     DVNLAVMAAADAWRDWRSASLSKRTSVMFAMRELLHQRSDEVAAAITSEHGKVLSDAA
                     GEVARGLEVVEFACGIPQLLKGGFSENVSTKVDVYSIRQPVGVVGVISPFNFPAMVPL
                     WFVPIAIACGNAVVLKPSEKDPSAANLLAELFAEAGLPDGVLNVVHGDKEAVDALLNH
                     PDVGSISFVGSTPVARYVYETGTKNGKRVQALGGAKNHMVVLPDADLDLAADAAVNAG
                     FGSAGERCMAISALVAVDPVGDELVERIRSRMATLRIGDGRRGCDMGPLVTRPHRDKV
                     ASYLDAGVSAGATLVVDGRDTPIDGDPDGFWLGPSLLDHVTPPMSCYTDEIFGPVLSV
                     LRSPSYDAALALVNASPYGNGTAIFSNDGG"
BASE COUNT          377 a          736 c          743 g          413 t
ORIGIN      
        1 tcttcacctc ctcgggcact gaggcgaacg aagcggcact gttgctggcc acgcagtacc
       61 ggcgcagcaa ccaggtgctg gcgctgcgca acagctatca cgggcgatcc tttgccagca
      121 tcgcgatcac cggtaaccgc ggctggagcg catcgagcct gtcgccggtg aacgtcagct
      181 acgtgcaaag cggctaccgc taccgcagcc cgttcgggca tctgtcggac gccgacttca
      241 tcgccgcgtg cgccgacgac cttcgtgacg tcatcaccac aaccacctcg ggcgacgttg
      301 cgtgcatgat tgccgagccg atccaaggtg tcggcggctt cgcgaccccg cccgacggca
      361 tgttcgcggc gttcaaggaa atcctcgact cctacggcat tctgttcatc tccgatgagg
      421 tgcagaccgg ctggggtcgc accggcgaac acttctgggg gatccaggcg cacggtgtgg
      481 tgcccgacgc gatgacgttc gccaagggcc tcggcaacgg gacgtcgatc ggtggcgttg
      541 ttgcgcgtgc cgagatcatg gactgcctaa atgccaattc catctccact ttcggcggca
      601 acccgcttgt cactgccgcc gccaacgcca ccctcgacta cgtgttgtcc cacgacctac
      661 aagcgaatgc ggccaaggtc gggaccgcct tgctcgaggg catccgtggc cttcagcaca
      721 cctatccgtt cgtcggtgac gttcgcggca agggactcat gatcggcgtc gaactcgtcg
      781 tgccgggctc gaagacgccc gacccaggcg cagcgacgcg gatgatggaa gagacgcgcg
      841 agcgtggttt gttgatcggc aaaggtggcc tccatggcaa cgtcctgcgc atcgcccctg
      901 cattgaccgt cacggaggcc gaggcggcag aggggctcga cgtgttggcg aagtcgttcg
      961 ccgccgtcga gtccgcgaat atcccggccg cggcaggcgc cagatgagcg agatccgtcg
     1021 catcacccat tggatcgggg gaaagccgca taccggcgag tcggcccgcc acggcgacgt
     1081 cttcgacccc gcgacgggtc aggtcactgg gcacgtcgac ttcgcgtcgt ccgaggacgt
     1141 caatcttgct gtcatggccg cagcagacgc gtggcgcgat tggcgttcgg catcgctgtc
     1201 gaagcgcacg tccgtcatgt tcgcgatgcg cgagcttttg caccagcgca gcgatgaggt
     1261 cgccgcggct attacctccg agcacggcaa ggtgctgtcc gatgccgcgg gcgaggtggc
     1321 cagagggctc gaggtcgtcg aattcgcgtg cggcatccca cagttgctca agggcggctt
     1381 ttcggagaac gtgtcgacga aggtcgacgt ctactcgatc cgccaacctg tcggcgtcgt
     1441 cggcgtcatc tcgccgttca acttcccggc catggtgccg ctgtggttcg taccgatcgc
     1501 gatcgcgtgc ggcaacgcag ttgtgctgaa gccaagtgag aaggatccgt cggcggcaaa
     1561 tctgctcgcc gagctgttcg ccgaggctgg tctccccgac ggcgttctga atgtcgtaca
     1621 tggtgacaag gaagctgtcg acgcgctgct gaaccacccc gacgtcgggt cgatctcgtt
     1681 cgtgggttcg acaccggtcg cgcgctacgt ctatgagacc ggtacgaaga acggcaagcg
     1741 ggtacaggcg ctcggcggcg cgaagaacca catggtggtg ctgcccgacg ctgatctcga
     1801 tctcgcagcc gacgccgctg tcaatgctgg ctttgggtcc gcgggcgagc gttgcatggc
     1861 gatctccgca ctagtcgccg tcgacccagt cggcgacgag ctcgtcgaac gcatcaggag
     1921 ccgcatggcg acgttgcgca tcggcgatgg ccgccgcggt tgcgacatgg gcccgctggt
     1981 caccagaccg catcgcgaca aggtcgcgtc ctatctcgat gcgggagtgt ccgctggtgc
     2041 gaccctggtc gtcgatggtc gagatacgcc catcgacggc gacccggacg ggttctggct
     2101 cgggccctcg ttgctcgatc acgtgacgcc gccgatgtcg tgctacaccg acgagatctt
     2161 cggtccggtg ctgtcggtct tgcgctcgcc cagctacgac gccgcgctcg ctttggtgaa
     2221 cgcgagcccg tacggcaacg gcactgcgat atttagcaac gacggcgga
//
LOCUS       DATCBJ010000150         2134 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_285891, whole genome shotgun sequence.
ACCESSION   DATCBJ010000150 DATCBJ010000000
VERSION     DATCBJ010000150.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2134)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2134)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2134
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_285891"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(18..383)
                     /locus_tag="VII50_03065"
     CDS             complement(18..383)
                     /locus_tag="VII50_03065"
                     /inference="COORDINATES: protein motif:HMM:NF022742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3099 domain-containing protein"
                     /protein_id="HEY5049853.1"
                     /translation="MARRVRDGGQEDDPPVYLVTAARPGHSQEMSGRLRRYLISMTIR
                     VVFLVLAIFFFTGWLRVVGVAAAVVLPWIAVVMANAGPVGDGAQPEFVSTDRIGIDGP
                     PVTQIGHSTEVGHSTEGKP"
     gene            438..650
                     /locus_tag="VII50_03070"
     CDS             438..650
                     /locus_tag="VII50_03070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dodecin"
                     /protein_id="HEY5049854.1"
                     /translation="MSDRTYRITEIVGTSGESVQHAVRNGVRRASQTIRHLDWFEVTG
                     IRGHIVEGEVEHFQVTMKVGFRLEDV"
     gene            complement(657..2063)
                     /locus_tag="VII50_03075"
     CDS             complement(657..2063)
                     /locus_tag="VII50_03075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase TldD-related protein"
                     /protein_id="HEY5049855.1"
                     /translation="MSDQTPQETVERALAIAAGIAGVDGCVVLADESSSANLRWANNT
                     LTTNGVTRGRRLTVIASVKGADGVAAGVVSRSSVRTADLDDLVRAAVTAAGENTPAED
                     AGALVEPTHPVDPAWHDGPQETSAAIFEGFVGDLGAALTQAARQDRLLFGFAEHVVNS
                     SFMGSSTGLRLRHDQPTGKLEMNAKSGDLARSAWSGAATQDFTDIDIGRESAALAQRL
                     EWAARRVDLPAARYETIIPSGALADLLIYMYWSAGARDAHDGRTVFSKPAGATRVGES
                     LSSLPLTLRSNPAELGLSCIPFVVAHASSAESSVFDNGLDLSPTSWIDKGRLNSLIQT
                     RHTAALTGLPVTPAIDNLIFEQAGATASLDDMIHSTERGLLLTCLWYIRAVDPQTLLL
                     TGLTRDGVYLVEGGEVVGAVNNFRFNESPVDLLDRITEVGAARRCLCREWNDYFTRTA
                     MPPVRVADFNMSTVSQAS"
BASE COUNT          361 a          708 c          714 g          351 t
ORIGIN      
        1 ggggaagagg tccgctgtca cggcttccct tccgtggagt gcccgacttc cgtggagtgg
       61 ccgatctgag tgaccggcgg gccgtcgatc ccgatccgat cagtcgagac gaattccggt
      121 tgtgcaccgt cgccgacggg cccggcgttc gccatcacga cggcgatcca tggcagcacg
      181 accgcagcgg ccaccccaac gacgcgcaac cacccggtga agaagaagat tgctagcacc
      241 aaaaagacca cccggatcgt catcgatatg agataacgcc gaaggcggcc ggacatttcc
      301 tgcgagtggc ccggacgggc ggccgtgact aggtagaccg gcggatcgtc ctcttgcccg
      361 ccgtcccgca cccgtcttgc cacttctcta ccgtacgccg cgatgatggg ggctccgcta
      421 gcgagcagga ggtttccatg agcgaccgca cctaccggat caccgaaatc gtcgggacgt
      481 cgggcgaaag cgtccaacat gccgtgcgca acggcgtccg acgggcatca cagacgatcc
      541 gtcatctgga ttggttcgag gtgaccggga tccgcggtca catcgtcgaa ggcgaagtcg
      601 agcactttca ggtcaccatg aaggtcgggt tccgtctgga agatgtctga cgaacgtcag
      661 gaggcctggc tgacagtgct catattgaag tcggcaacgc ggaccggggg catcgccgtc
      721 cgggtgaagt agtcgttcca ttcgcgacac aggcaccgcc gcgccgcgcc aacctcggtg
      781 atgcgatcca gcaggtcgac ggggctctcg ttgaaccgga agttgttgac ggcgccgacc
      841 acctcaccgc cctcgacgag gtacacgccg tcgcgagtca aaccggtgag cagcagcgtc
      901 tgcggatcga ccgcccggat gtaccacagg cacgtcagca gcagcccgcg ctcggtgctg
      961 tgaatcatgt cgtcgaggct cgcggtggcg ccggcctgct cgaagatgag gttgtcgatc
     1021 gccggcgtca cgggcagacc cgtcaacgcc gcggtgtgcc gggtctggat cagcgagttc
     1081 aagcgtccct tgtcgatcca cgacgtgggg gacagatcga ggccgttgtc gaagacagaa
     1141 ctctccgcgc tcgacgcgtg ggcgaccacg aaggggatgc acgaaaggcc cagctctgca
     1201 gggttgctgc gcaacgtcaa cggcaggctg ctcagtgact caccgacccg cgtcgcgccc
     1261 gcgggtttgg agaacacggt gcggccgtcg tgggcgtcgc gggctccggc ggaccagtac
     1321 atgtagatca gcaagtcggc gagcgcgccg gacggaatga tggtttcgta gcgcgcggcc
     1381 ggcaggtcga cgcgtcgggc cgcccactca aggcgctgcg ctagcgcagc gctctcgcgt
     1441 ccgatgtcga tgtcggtgaa gtcctgggtt gctgctcctg accaggccga ccgcgccaga
     1501 tcgcccgact tggcgttcat ctcgagcttg ccggtgggtt gatcgtggcg cagccgcagg
     1561 ccggtcgagc tacccatgaa gctggagttc accacgtgct cggcgaagcc gaagagcagt
     1621 cggtcctgtc gtgccgcctg tgtgagcgcc gccccgagat cgccgacgaa cccctcgaag
     1681 atcgccgccg aggtctcctg ggggccgtcg tgccacgccg gatcgacggg gtgcgtgggc
     1741 tcgaccagcg ccccggcgtc ctcggccggg gtgttctcac ccgcggctgt gactgcggcc
     1801 cgcacgagat cgtcgagatc ggccgtgcgc acagagcttc gcgacacgac gcctgctgcc
     1861 acgccgtccg ctcccttgac cgaagcgatc accgtcaacc gccggccacg cgtaaccccg
     1921 ttggtcgtca acgtgttgtt cgcccagcgc aggttggcgc tgctcgactc gtcggcgagc
     1981 actacgcagc catcgacgcc ggcgatgcct gcggcgatcg cgagcgcgcg ctcgacggtc
     2041 tcctgcggtg tctggtcgct catctagcgg ccaccttcct gcacggtgtt caggatcttg
     2101 acgccgcgaa acaccgctga cgggcacccg tggc
//
LOCUS       DATCBJ010000151         2212 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_95338, whole genome shotgun sequence.
ACCESSION   DATCBJ010000151 DATCBJ010000000
VERSION     DATCBJ010000151.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2212)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2212)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2212
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_95338"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..312)
                     /locus_tag="VII50_03080"
     CDS             complement(<1..312)
                     /locus_tag="VII50_03080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY5049856.1"
                     /translation="MADSPILALRGINKSFGAIQVLHSVDFNVFPGEVMALVGDNGAG
                     KSTLVKCVGGIYPVDSGEFLFDGNPIAIHGPRDAAAYGVEIVYQDLALCDNLDIVQNM
                     FL"
     gene            complement(512..1711)
                     /locus_tag="VII50_03085"
     CDS             complement(512..1711)
                     /locus_tag="VII50_03085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEY5049857.1"
                     /translation="MNKTAIRLIAVAAVAGLGIAGCSSSKKTTAPTSSAPAVAATTSA
                     PAAATSAPAAATSAPAASSAPASSAPAATGATVGVILPDTQSSTRWETADRPALNKAF
                     SAAGIKADIQNALGDNAKFQSIAQGMIQEGVKVLIITSIDPATSIQVETEAKAANIPV
                     IDYDRLVLGGSAAYYASYDGVQVGVEQGQCLVDAMAKIGKPTGRIIMIDGSPTDNNAT
                     LFGQGAHSVIDKSTLKKVSEQAIPNWDNTQAGKTFQQLLTAAGNKIDGVYVMNDGMAG
                     PVETVLKASGLSGIPVTGQDATAAGLQAILSGTQTCTIYKDATAEATAAATLAVDLIK
                     GTTPSGLTTSNDPQGKRSVPSILLKPELITIATVGDPIKSGAVPYASVCTGTFVALCT
                     KAGIAAS"
     gene            complement(1904..>2212)
                     /locus_tag="VII50_03090"
     CDS             complement(1904..>2212)
                     /locus_tag="VII50_03090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5049858.1"
                     /translation="RPQAISQLHADLVMLLVGVAAALAVCVTAVGASAAARRTTRVLI
                     GVLVAQAAVGFAQYFSGLPVPLVELHVTGAAVLAATATAVVLSLQNAQQKAPSRQKPL
                     "
BASE COUNT          377 a          667 c          755 g          413 t
ORIGIN      
        1 gaggaacatg ttctgcacga tgtcgaggtt gtcgcacagc gcgaggtctt gatagacgat
       61 ctcgacgccg taggcagccg catcacgtgg gccgtggata gcgatcgggt ttccgtcgaa
      121 caagaactcc ccggagtcga cggggtagat cccgcccacg cacttgacga gagtcgactt
      181 gccggcgccg ttgtcgccca ccagcgccat cacttcgcca gggaagacat tgaagtcgac
      241 gctgtgcagg acctggattg cgccaaagct cttgttgata ccccgtaggg cgagaatggg
      301 cgaatcggcc accgtgagcc tctctctagg agccccgatg tgagcgcgcc aagatcgggg
      361 aaagaacgga cgtcgccaag atagaccgcc ggacacgtta cggcgagatg tagatcacgg
      421 attcactgac acatcacaga cgacaagaaa ccgatggcgt ggccggggtt accggccacg
      481 ccaccggact tcttactggc gaagatgcag cttaggaagc tgcgatcccg gccttggtgc
      541 agagggcgac gaacgtgccg gtgcagacgg acgcgtacgg gaccgcgccg gacttgatcg
      601 ggtcgccgac ggtggcgatc gtgatcagtt caggcttgag caggatcgaa ggaacgctgc
      661 gcttgccctg cgggtcgttc gacgtcgtca ggcctgaggg cgtggtgccc ttgatcaggt
      721 cgacggccag cgtcgcggcc gcggtggcct cggcggtggc gtccttgtag atggtgcagg
      781 tctgagtacc cgagaggatg gcctgcaagc cagctgcggt tgcgtcctgg ccggtcaccg
      841 gaatgccgga aaggccgctc gccttgagga cggtctcaac cggaccggcc ataccgtcgt
      901 tcatcacgta cacgccatcg atcttgttgc cggcggcggt gagcagctgc tggaaggtct
      961 tgccggcctg ggtgttgtcc cagttcggga tggcctgctc ggaaaccttc ttcaaggtgc
     1021 tcttgtcgat caccgagtgc gcgccctgac cgaacagcgt cgcgttgttg tcggttggcg
     1081 agccatcgat catgatgatg cgaccggtgg gcttgccgat cttcgccatc gcgtccacga
     1141 ggcactggcc ctgctcgacg ccgacctgga cgccgtcgta ggacgcgtag tacgccgcgc
     1201 taccaccgag aacgagacgg tcgtagtcga tgaccgggat gttggcggcc ttcgcttcgg
     1261 tctcaacctg aatggacgtc gcggggtcga tcgacgtgat gatcaggacc ttgacgcctt
     1321 cctggatcat gccctgcgcg atgctctgga acttggcgtt gtcaccgagt gcgttctgga
     1381 tgtcggcctt gatgccggct gccgagaacg ccttgttcag ggccggacgg tcggcggtct
     1441 cccaccgggt cgacgactgg gtgtccggca ggatcacgcc gacggtcgca ccggtcgcag
     1501 ctggggcgct cgaagcgggg gcggacgatg ccgctggggc gcttgtggcg gccgcgggtg
     1561 cggacgttgc cgctgccggg gcgctcgtgg tggcggcgac ggccggcgca gagctggttg
     1621 gcgcagtggt cttcttgctg ctgctacagc cggcaatacc cagcccggct accgccgcga
     1681 cggcaatgag cctgatcgca gtcttgttca tgcacgatcc cctttttgct cctgggcgca
     1741 ggacgtggtg ccctacagga ctgcccagtt gttgtgccgc acaacatatg caccgaggac
     1801 ccgtcaacgt aagggcaaca gcgcccatct ttgtgttcgg aatggatctg ttacataggc
     1861 gtgaccctgc gggtacacgc ggcaaggaca tctttatgcc gttttataat ggtttctgac
     1921 ggctcggtgc cttctgctgc gcgttctgta acgagaggac gacggcggtg gcggttgctg
     1981 cgagcaccgc ggcgccggtc acatgcaact cgactagcgg caccggcagt ccgctgaagt
     2041 actgggcgaa gccaacggct gcctgcgcca cgagcacgcc gatgagtacc cgcgtcgttc
     2101 gtcgggccgc agcactcgca ccaacggccg tgacgcacac cgcgagggcc gccgccacgc
     2161 cgacgagcag catcacgagg tcggcatgca gctggctgat tgcttggggg cg
//
LOCUS       DATCBJ010000152         2525 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_466559, whole genome shotgun sequence.
ACCESSION   DATCBJ010000152 DATCBJ010000000
VERSION     DATCBJ010000152.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2525)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2525)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2525
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_466559"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..347)
                     /locus_tag="VII50_03095"
     CDS             complement(<1..347)
                     /locus_tag="VII50_03095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HEY5049859.1"
                     /translation="MADVEQLTAPVAFHAEGPVWSPSWGGLRWVDMFAGDVLSMGLDG
                     AAGTFGAISRRHVGAIAAALRPRRGGGAVIAVERGFLLERADGELDPLPDLWADPSLR
                     MNEGSCDPDGRFY"
     gene            complement(382..573)
                     /locus_tag="VII50_03100"
     CDS             complement(382..573)
                     /locus_tag="VII50_03100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5049860.1"
                     /translation="MVGADIAGERWRNALAELEYAKRHGSPADEVEQLVNGVIEARVA
                     MFHAGIARHRALPAYLSPA"
     gene            767..1108
                     /locus_tag="VII50_03105"
     CDS             767..1108
                     /locus_tag="VII50_03105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lsr2 family protein"
                     /protein_id="HEY5049861.1"
                     /translation="MAQRVSVNLVDDIDGGSAVETIYFSLDGVSYEIDLGKRNADRLR
                     AAMAAYSQAGRRLVRTTGRGRGRTVVQRRVEVAADPRAVRAWAKANRVAVPDRGRIPQ
                     AVLERYRAAGH"
     gene            1271..1663
                     /locus_tag="VII50_03110"
     CDS             1271..1663
                     /locus_tag="VII50_03110"
                     /inference="COORDINATES: protein motif:HMM:NF002377.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sec-independent translocase"
                     /protein_id="HEY5049862.1"
                     /translation="MPFDLSMWKLMVLGVIALVVFGPDRLPQLARDAGRTLRQLRELA
                     QTARTELKSELGDTVGEFDFSDLNPRTFVRKHLLDDLDGDLASAAPGRSPNGTTAAGA
                     AAAGGGPSPVPAAFGALTEPAPYDLDAT"
     gene            1798..2265
                     /locus_tag="VII50_03115"
     CDS             1798..2265
                     /locus_tag="VII50_03115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEY5049863.1"
                     /translation="MKVKEIMKAPVYTVGVDVPTADIAKLLIQHRISAVPVVDASGAV
                     VGLVSEHDLISRDGPTALDVMSPGIVSVTEDTDVDDVRHLLVDRRIRRVPVLSGGRLV
                     GIVSRADIVALIAMEWVCEVCGTQARGEHPPASCPTCAADTVRFVHLQQPPGA"
     gene            2333..>2525
                     /locus_tag="VII50_03120"
     CDS             2333..>2525
                     /locus_tag="VII50_03120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HEY5049864.1"
                     /translation="MPPAQQKTATRAKTSTKTTARSAFPGEPVERLRDYYREMQLIRR
                     FEERASEMYQRAKIGGYCHL"
BASE COUNT          436 a          872 c          835 g          382 t
ORIGIN      
        1 caatagaagc ggccgtcagg gtcgcaactg ccctcgttca tgcgcaggct cggatcggcc
       61 cacaggtctg gcagcgggtc caactcgccg tccgcgcgtt cgagcaagaa cccacgctca
      121 acggcgatga cggctccgcc gccgcgtcgc gggcgaagcg ctgccgcgat cgcaccgacg
      181 tgtcgtcggc tgatggcgcc gaaagtgccc gccgcgccgt ctagacccat ggacaggacg
      241 tcgccggcga acatgtcgac ccagcgcagt ccgccccagg acggcgacca gacagggccc
      301 tcggcgtgaa acgccaccgg tgcggtgagc tgttccacgt cggccatgac gcgcgatgct
      361 acggcagcgc gatggccggg actacgcggg cgacaggtag gcaggcagtg cccggtggcg
      421 ggcgattccg gcgtggaaca tcgcgacccg agcctcgatc acgccgttga caagctgctc
      481 gacttcatcg gccggggatc cgtgccgctt cgcgtattca agctccgcga gggcattgcg
      541 ccagcgctcg cccgcgatgt ccgcgccgac cacgcctcgc ctcccgcctg aagggattag
      601 tctgtcttaa tccaatttca tatgccatca gacgaatgtc cataggtctt tcgccaggtc
      661 ggttgtccag ccgtacgacc ctgcccaagg acccgtcggg ctggcgccat agccgaggct
      721 gagatgattg gctcggcagt gcagggtccg aatagaggga caggcgatgg cgcaaagggt
      781 ctcggtcaat ctcgtcgacg acatcgacgg cggaagcgcg gtggagacga tctatttctc
      841 cctcgatggc gtcagctatg aaatcgacct cggcaagcgc aacgccgacc gcctgcgagc
      901 cgcgatggcc gcctatagcc aggccggacg ccggttggtg cggaccaccg gacgcggccg
      961 tggtcgaacc gtggttcagc gccgcgtcga ggtggccgcc gacccgcggg ccgtccgtgc
     1021 gtgggcgaag gccaaccggg ttgccgtccc tgatcgcggc cggattccgc aagccgtctt
     1081 ggagcgctac cgcgcggcag gtcactgagc gctacccgcg gcaggtcact aagggatccg
     1141 aacggcaggt cactgaggga tccgaacggc tccgtcgcca cgaaccgccg gtaacctaac
     1201 actgacacgc gcgctggctc cgggggctcg tccctcgcca gctcgacagc gtcttgacgg
     1261 gaggtgcgat gtgccgttcg acctgagcat gtggaagttg atggtcctcg gcgtcatcgc
     1321 actggtcgtc ttcggacctg acagattgcc gcaattggcg cgtgacgcag gtcgcacgtt
     1381 gcgacagctg cgggagctgg cgcaaaccgc gcgaaccgag ttgaaatccg agctcggtga
     1441 cacggtcggt gagttcgact tctccgacct gaacccgcga acgttcgtgc gcaagcacct
     1501 gctcgacgac ctcgacggcg acctcgcgtc tgccgcgccc ggccgatcgc caaacgggac
     1561 gacggcggct ggcgcagctg cggccggtgg cggtccatcg ccggtccccg cggcattcgg
     1621 tgcgctcacg gagccagctc cgtacgacct cgacgcgacc tgacgcaacg acaccgtcgg
     1681 ccggtaggct cggctacgcc aatcgaccac aagtcgtcgg cgcgaccctt ctgccgaacc
     1741 gggccaaagc tcggccgtcg ctgggcgccc ggcgcggaga agcgcggggc gagtgagttg
     1801 aaggtcaaag agatcatgaa ggcgccggtc tatacggtcg gcgtcgacgt acctacggcc
     1861 gatattgcca agctcctcat ccagcaccgc atcagcgcgg tgcccgtcgt cgacgcctcc
     1921 ggggccgtcg tcggcctcgt gagcgagcac gacctgatct cccgtgacgg cccgacggcg
     1981 ctcgacgtga tgtcgccggg catcgtcagc gtgaccgagg acaccgacgt cgacgacgtc
     2041 cggcatctgc ttgtcgatcg gcggattcgc cgtgtccccg tgctgtccgg aggccggctg
     2101 gtcggcatcg tcagtcgcgc cgacatcgtc gcgctgatcg ccatggaatg ggtgtgcgag
     2161 gtgtgcggca cacaggcgcg cggcgagcac ccaccggcgt cctgtccgac ctgtgcagcc
     2221 gacaccgtgc gcttcgtcca cctgcaacaa ccacccggcg cgtaggtagt aacacccaac
     2281 tgcgcagccc gaaccagtcc ggcaccaccg cttgagccac gaaggagacg ccgtgccgcc
     2341 cgcacagcag aaaaccgcca cccgcgcgaa gaccagcacc aagaccaccg cgcgttcggc
     2401 gtttcccggc gaacccgtcg agcggctgcg cgactactac cgcgagatgc agctcatccg
     2461 gcggttcgag gaacgcgcgt ccgagatgta ccagcgggcc aagatcggcg gatattgcca
     2521 cctca
//
LOCUS       DATCBJ010000153         2188 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_279260, whole genome shotgun sequence.
ACCESSION   DATCBJ010000153 DATCBJ010000000
VERSION     DATCBJ010000153.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2188)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2188)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2188
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_279260"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..261
                     /locus_tag="VII50_03125"
     CDS             <1..261
                     /locus_tag="VII50_03125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5049865.1"
                     /translation="PIWAPSTILIEDGLRRSGFVALADEISGRFRALCEQSGFAENFD
                     AETGTGLRDHAYTWTASSYLILAAAHAQRHATNSMSAEGSSR"
     gene            complement(422..1492)
                     /locus_tag="VII50_03130"
     CDS             complement(422..1492)
                     /locus_tag="VII50_03130"
                     /inference="COORDINATES: protein motif:HMM:NF012742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEY5049866.1"
                     /translation="MPSKLGSGRPGMMDVALLAGVSHQTVSRVINMQSGVKATTRIRV
                     QAAIEELGYRRNHAARTLATGRSQTLGVVMLNSTLYGPASTLYGIEQAALSARYLVTV
                     ASARSKDRSAVQEAIGRLVDQAVEGIVMIAPLVSVGEALAALPTDLPVVMIEGDLRTD
                     VDVVTVDQTAGAKAATQHLLDCGHATVWHVAGPAEWVEARQRTAGWHAALEEAGADIP
                     PPLSGDWSARSGFAAGRALARIPDLSAVFVANDSMALGVMRAMYDANRRVPADVSIVG
                     FDDIPEAAYFTAPLTTVRQDFDEVGRSALALLVDQMRSGPRASKRIVVTTTLAVRSSA
                     GPPNASRATTNSATKLARSAER"
     gene            complement(1967..>2188)
                     /locus_tag="VII50_03135"
     CDS             complement(1967..>2188)
                     /locus_tag="VII50_03135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009077777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEY5049867.1"
                     /translation="LWAWSDFIFANTLDGGGQHLPITLGIYHYIGNNDQQWSAIMATA
                     VVASIPATVLLVVAQRYVAAGVTAGAVKD"
BASE COUNT          396 a          660 c          711 g          421 t
ORIGIN      
        1 ccgatctggg caccatcgac gatcctcatc gaggacggcc tgcggcgaag cggattcgtc
       61 gcgttggcgg acgagatcag cggacggttc cgagcgctgt gcgaacagtc cgggttcgcc
      121 gaaaacttcg acgccgaaac aggcacagga ttgcgcgacc acgcctacac gtggacggca
      181 agtagctatc tgatcctggc cgccgcgcat gcgcagcggc atgcgaccaa ctctatgtct
      241 gcagaagggt cgtctcggtg agacaacctc gggtgagcgg tggtgatttt ctacggcgag
      301 ccaaccattg tgctggagtc ctcacctgct ggaagcgctt cgcgagagtg cgctgcgcat
      361 cgttcgcctc acctgtgccg gcgcatctga aagcggtgca ctgaacagta aggcacgcca
      421 actagcgttc ggctgaacgc gcgagttttg ttgctgagtt ggtcgttgca cgtgacgcat
      481 tcggtggccc ggcgctgcta cgcacggcaa gcgtcgtcgt aacgactatg cgcttcgacg
      541 cgcggggtcc ggagcgcatt tggtcgacca ggagagccaa cgcgctgcgt ccgacttcat
      601 cgaagtcctg ccgcacagtc gttagcggag cagtgaaata ggcggcctcg ggaatatcgt
      661 caaagcccac gatgctcacg tcggccggta cccgccggtt ggcgtcatac atcgcccgca
      721 tcactccgag ggccatagaa tcgttcgcca cgaaaaccgc actgaggtcg ggtatgcgcg
      781 caagtgcgcg gcctgccgcg aatcctgagc gtgcgctcca atcgccgctt agcggtggag
      841 gtatgtcggc gcctgcctct tccaaggcgg cgtgccaccc tgcagtccgt tggcgcgcct
      901 cgacccactc ggccgggccc gccacatgcc acacggtggc gtgcccacag tcgagcaggt
      961 gttgcgtcgc agctttcgcg cccgcagttt ggtcgaccgt tacgacatcg acgtcagttc
     1021 ggagatcacc ttcgatcatg acgaccggca aatcggtggg cagggcagcg agtgcctcgc
     1081 ccacggagac gagtggcgcg atcatgacga tgccctcaac agcctgatcg acgagccggc
     1141 cgatcgcctc ttggaccgcg gaacgatcct tcgatcgcgc gctcgcgact gttacgaggt
     1201 accgcgctga gagcgcggct tgctcgatcc cgtacaacgt cgaagccggt ccgtagagtg
     1261 tgctgttcaa catgaccaca ccgagcgtct gtgaacggcc ggtcgctaac gtccttgcgg
     1321 catggttacg gcggtaaccc aactcctcga tcgcggcctg cacgcgaatg cgcgtcgtcg
     1381 cctttactcc ggactgcatg ttgatcacac gcgagaccgt ctggtgcgaa actccggcaa
     1441 gcaatgcgac gtccatcatc cccggccgac cgctgcctag tttcgacggc accgatacct
     1501 ctcttcgtta tcatttggac gtagcgcgga tcttgtttct attcgcccat aggtagtgat
     1561 gaacggcagt aacggtgctc gtgggtacgg cgaaacggct ccaccgggga tcgcgaatag
     1621 cgcggatgcg acacggttgc gtcgatgggc agcgtcgcgt gagcccgcac agcttgggca
     1681 ccgggacggt cttcaaacgc gcgaagttcg gctggttgtg cgacatggag caggatcgcg
     1741 ccgaggtaag tctgggttgt cggccgcctc gacgcaggtc gtcggcgtag acgcactcgg
     1801 cgagcacggg tgataactcg aaccatgagc cgtagtagct gaaccgaatg tcgggaccga
     1861 gaacgccggg ccaggtgggg cggtcatcag ggtggcactc actctctgtg cgtcttggaa
     1921 tcgggtcttg cgaggtcggg ttgctgttga tcagggggtg atcggcttag tccttgactg
     1981 ctccggctgt gaccccggca gcgacgtagc gttgggcgac gactaggagc accgtggccg
     2041 ggattgatgc gacgacggcg gtggccatga ttgcgctcca ttgctggtcg ttattgccga
     2101 tgtagtgata gatccccagc gtgatgggca gatgttgtcc gccgccatcc aaggtgttcg
     2161 cgaagatgaa gtccgaccat gcccatag
//
LOCUS       DATCBJ010000154         5115 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_476224, whole genome shotgun sequence.
ACCESSION   DATCBJ010000154 DATCBJ010000000
VERSION     DATCBJ010000154.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 5115)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5115)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5115
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_476224"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            50..661
                     /gene="rplE"
                     /locus_tag="VII50_03140"
     CDS             50..661
                     /gene="rplE"
                     /locus_tag="VII50_03140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016637787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HEY5049868.1"
                     /translation="MTATTTETPSTGTSSAGTPSPRTAPRLKQRYRDEISAQLREQFG
                     YSNVMQTPGLTKVVVNMGVGEAARDAKLIDGAVRDLTTITGQKPQVTRSRKSIAQFKL
                     RENMAIGCHVTLRGDRMWEFLDRLLALALPRIRDFRGLSPRQFDGHGNYTFGLTEQVM
                     FHEIDQDRIDRFRGMDITVVTTASTDDEGRALLRLLGFPFKEN"
     gene            744..929
                     /locus_tag="VII50_03145"
     CDS             744..929
                     /locus_tag="VII50_03145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003948630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HEY5049869.1"
                     /translation="MAKNALKIKAAAKPKFAVRGYTRCNRCGRSHSVYKKFGICRICL
                     REMAHRGELPGITKSSW"
     gene            1000..1407
                     /gene="rpsH"
                     /locus_tag="VII50_03150"
     CDS             1000..1407
                     /gene="rpsH"
                     /locus_tag="VII50_03150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003974254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HEY5049870.1"
                     /translation="MMMTDPIADMLTRVRNANHAYHDNVPIPYSKIKTHVADILKEQG
                     YIAGWHVEDVEGHAAAKNLVVELKYGPNRERSIAGVRRVSKPGLRVYAKGTNLPRVLG
                     GLGIAIISTSGGLLTDKQCAKRGVGGEVLAYVW"
     gene            1425..1964
                     /gene="rplF"
                     /locus_tag="VII50_03155"
     CDS             1425..1964
                     /gene="rplF"
                     /locus_tag="VII50_03155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006245132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HEY5049871.1"
                     /translation="MSRIGRLPIPVPSGVDVDITDRLVTVKGPKGTLTHTLADPIVIE
                     KADDGSLVVSRPDDEKRSKERHGLSRTLVANMVIGVTDGFAKTLEIVGTGYRVQAKGS
                     DLEFALGFSHPVNIPAPDGITFEVQAPTRFVVRGIDKQQVGEVAANIRKLRKPEPYKG
                     KGVRYQGEVVRRKVGKAGK"
     gene            1967..2356
                     /gene="rplR"
                     /locus_tag="VII50_03160"
     CDS             1967..2356
                     /gene="rplR"
                     /locus_tag="VII50_03160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HEY5049872.1"
                     /translation="MPVGLISRRKARGSTKSALRQRRHFRVRKRVTGTTARPRLVVNR
                     SSRHVFAQVVDDTTGRTLAYASTMEADVRAVAGDKTAKARKVGELVAKRAAAAGVGPV
                     VFDRGGYQYHGRVAAIADGAREGGLTL"
     gene            2409..3068
                     /gene="rpsE"
                     /locus_tag="VII50_03165"
     CDS             2409..3068
                     /gene="rpsE"
                     /locus_tag="VII50_03165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HEY5049873.1"
                     /translation="MPGPQRRGPGSGSGTGAGAAGGPGGSGSGGGDRRDRRDNRRPDG
                     AAEKTQYIERVVAINRVAKVVKGGRRFSFTALVVVGDGDGSVGIGYGKAKEVPAAIAK
                     GVEEAKKSFFKVPRIQHTIPHPITGEAAAGVVLLRPASPGTGVIAGGPVRAVLECAGI
                     HDVLSKSLGSDNAINVVHATVAALKGLMRPEEVAARRGLPLEDVAPAALLRARAAGMG
                     A"
     gene            3070..3252
                     /gene="rpmD"
                     /locus_tag="VII50_03170"
     CDS             3070..3252
                     /gene="rpmD"
                     /locus_tag="VII50_03170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009190145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HEY5049874.1"
                     /translation="MGRLKVTQTRSEIGGTSTQRNTLRTLGLKRMRDTVVKEDRPEIR
                     GMIRTVSHLVAVEEVD"
     gene            3294..3728
                     /gene="rplO"
                     /locus_tag="VII50_03175"
     CDS             3294..3728
                     /gene="rplO"
                     /locus_tag="VII50_03175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016911111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HEY5049875.1"
                     /translation="MKIHHLRPAPGSHKPKTRVGRGEASKGKTAGRGTKGTKARYQVP
                     ARFEGGQMPLYMRLPKLKGFTNRFRVEYQVVNLEWIAAHYPKGGAVTPEDLVAKGAVR
                     KGELVKVLGDGEIGVKLQVSAHGFSASAKEKIEAAGGSATKL"
     gene            3814..>5115
                     /gene="secY"
                     /locus_tag="VII50_03180"
     CDS             3814..>5115
                     /gene="secY"
                     /locus_tag="VII50_03180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020633325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HEY5049876.1"
                     /translation="MNYALLRAFGQAFRTPDLRKKMLFVLFIVLLFRLGSAIPAPGVS
                     EKNIHTCLVEVTGGNSANVYTLINLFSGGALLKLSILALGIMPYITSSIIIQLLVVVI
                     PRLERLKQEGQSGQAKLTQYTRYLTIGLAILQSSAFVALARSPGQIFTGCTTPIIPND
                     GWQVILTIVLTMTAGTAVIMWLGELITDRGIGNGMSILIFTQIAARFPSELNSIRQTK
                     GLFTFLMVILVGAVIIAAIVFVEQAQRRIPVQYAKRMIGRRMYGGTSTYLPLKVNQAG
                     VIPVIFASSLLYIPSLLTRVWSGNLGFTQFVTNYLSPNGHLTYLLLYAALIIFFTFFY
                     VAITFNPDEVAENMKKYGGFIPGIRPGRPTAEYLTYILSRITVPGATYLAVVAVIPEA
                     ALSIIGSSQQFPFGGTSVLIIVGVGLDTVKQIESQLMLRNYE"
BASE COUNT          970 a         1618 c         1658 g          869 t
ORIGIN      
        1 cggtgcggat ctgcaagaag tgcggcaagg aaatcatggt ggcgacgcca tgaccgcgac
       61 cacgactgag accccgagca cagggacgtc gagcgcaggg acaccgagcc cgcgcacggc
      121 gccgcgtctc aagcagcgtt accgcgacga gatctcggcc caactgcgtg agcagttcgg
      181 ctactcaaac gtcatgcaga ctcccggcct gaccaaggtc gtcgtgaata tgggtgtcgg
      241 cgaggctgct cgcgacgcga agctcatcga cggagccgtc cgcgacctga ccacgatcac
      301 tggtcaaaag ccgcaggtca cccgctcgcg gaagtcgatc gcccagttca agctgcgcga
      361 gaacatggcg atcgggtgcc acgtcacgct gcgcggcgac cggatgtggg agtttctcga
      421 ccggctgctc gcacttgcgc tgccgcgcat ccgtgacttc cgcggattgt cgccgcgcca
      481 gttcgacggc cacggcaact acacgttcgg tctcaccgag caggtcatgt tccacgagat
      541 cgaccaggac cggatcgatc gcttccgtgg catggacatc acggtggtca cgaccgcgag
      601 cacggacgac gaggggcgcg cgttgcttcg cctgctcggc ttccccttca aggagaacta
      661 gtgagcgggc atcgaaggcg cgaagcgccg aggagcggaa cgaacactgc gatgagcgct
      721 tgcgcgaaga gcgagggata aaagtggcga agaatgctct gaagatcaag gcggctgcga
      781 agccaaagtt cgcggtgcgt ggctacacgc ggtgcaaccg atgcggccgg tcgcattcgg
      841 tgtacaagaa gttcggcatc tgccgcatct gccttcgcga gatggcgcac cgcggcgagt
      901 tgcctggcat caccaagagc agctggtaaa ccccactacg ccgcaggtcg ccagagctat
      961 ggcgaggcga aaccacggcg agaaaggcca tgtggccaca tcatgatgac tgacccgatc
     1021 gcggacatgt tgacgcgagt gcgcaacgcg aaccacgcgt accacgacaa cgtgccgatt
     1081 ccgtactcga agatcaagac gcacgtcgcc gacatcctca aggagcaggg ctacatcgcc
     1141 ggttggcacg tcgaggacgt cgaaggccac gccgcagcga agaacctggt cgtcgagctg
     1201 aagtacggcc ccaaccgtga gcggtcgatc gccggcgtcc ggcgggttag caagcccggc
     1261 ctgcgcgtct atgccaaggg caccaacttg ccgcgcgtcc tcggcggcct cggcattgcc
     1321 atcatctcga catccggcgg gctgctgacc gacaagcagt gcgcaaagcg tggggtaggc
     1381 ggggaagtcc tcgcctacgt ctggtaacag gaagagacga actcatgtcc cgaatcggaa
     1441 gacttccgat ccctgttccc agcggggtcg acgtcgatat caccgaccgt ctggtcacgg
     1501 tcaagggccc gaagggcacc ttgacgcaca cgctcgccga tccgatcgtc atcgagaagg
     1561 cggacgacgg ttcgctggtc gtcagtcgcc ccgatgacga gaagcggagc aaggaacgcc
     1621 acggccttag ccgcactttg gtcgccaaca tggtgatcgg ggtgaccgat ggcttcgcga
     1681 agaccctcga gatcgtcggt accggttacc gcgtacaggc aaagggctcg gacctcgagt
     1741 tcgccctcgg cttctcgcac ccggtcaata tcccggcacc cgacggcatc accttcgagg
     1801 tgcaggcgcc gacgcggttc gttgtgcgcg gcatcgacaa gcagcaggtc ggtgaggtcg
     1861 cagccaacat ccgcaagctg cgcaagccgg agccctacaa gggcaagggc gttcgctacc
     1921 agggtgaagt tgtgcgtcgc aaggtcggaa aggctggtaa gtagtcatgc ctgttggatt
     1981 gatcagtcgc cgcaaggcgc gcggatccac caagtccgcc ctgcgtcagc gccgccactt
     2041 ccgggtgcgc aagcgcgtga ccggtaccac tgctcgtccg cgcctcgtgg tcaaccgttc
     2101 gagccggcac gtttttgcgc aggtcgtcga cgacacgacc ggccgcacgc tcgcctacgc
     2161 gtcgacgatg gaagccgatg tgcgcgcagt tgcgggtgac aagaccgcca aggctcgcaa
     2221 ggtcggcgaa ctcgttgcca agcgtgccgc cgccgcgggc gtcggtccgg tggtcttcga
     2281 ccgtggtggg tatcagtacc acgggcgcgt cgcggcgatc gccgacggcg cccgcgaggg
     2341 cggtctgacg ctgtgacccc gaaccgaatg actgtgaaat gcgtgaccac tgagatgagg
     2401 aacatctaat gccaggacct cagcgtcgtg gacccggaag cggcagcggt actggtgccg
     2461 gtgccgccgg tggacccggc ggaagtggca gtggcggcgg cgatcgccgt gaccgccgtg
     2521 acaatcgtcg tcccgatggc gcggccgaga agacccagta catcgagcgc gtcgtcgcga
     2581 tcaaccgcgt cgccaaagtc gtgaagggcg gccggcgctt cagcttcacc gcgcttgtgg
     2641 tcgtcggtga cggcgacggc agtgtcggca tcggctacgg caaggccaag gaagtgcccg
     2701 cagcgatcgc gaagggtgtc gaagaggcca agaagagttt cttcaaggtg ccgcgcatcc
     2761 agcacacgat tccgcacccg atcaccggcg aggccgcggc cggcgttgtg ctgttgcgcc
     2821 cggccagccc cggtaccggc gtcatcgccg gcggacccgt tcgcgccgtg ctggagtgtg
     2881 cgggcattca cgacgtgctg tcgaagtcac tcggctccga caacgcgatc aacgtggtgc
     2941 acgcgaccgt ggcggcactg aagggcctga tgcgtcccga ggaagttgcg gctcgccgtg
     3001 gcttgccgct tgaagatgtc gcacccgccg cattgttgcg tgcccgtgcg gccgggatgg
     3061 gagcctgaca tgggccggct gaaggtgacc cagacccgat cggagatcgg cggcacaagt
     3121 actcagcgca acaccttgcg cacacttggc ctgaagcgaa tgcgcgacac cgtggtcaaa
     3181 gaggaccgtc ccgagatccg cggcatgatc aggaccgttt cgcacctggt agccgttgag
     3241 gaggttgact gacgatggct gacaccacca agacagaacg ggcgagtgct gtgctgaaga
     3301 ttcaccacct gcggcccgcg cctggttccc acaagccgaa gacccgggtc ggtcgaggcg
     3361 aggcgtccaa gggcaagacc gccggtcgtg gcaccaaggg caccaaggcc cgttaccagg
     3421 tgcccgcgcg cttcgagggc ggccagatgc cgctctatat gcggctgccc aagctgaagg
     3481 gctttaccaa ccgtttccgc gtggaatacc aagtcgtgaa cctcgagtgg atcgccgcgc
     3541 actaccccaa gggtggtgcg gtgaccccgg aagacctggt cgccaagggt gccgtccgaa
     3601 agggcgagct ggtcaaggtc ctcggcgacg gcgagatcgg cgtgaagctt caggtcagcg
     3661 cgcacggttt ctcggcgtcg gcgaaggaga agatcgaggc ggccggcggc agcgccacga
     3721 aactgtagga cgactcgtgc gttacagtca cggagcgggc gccggtgact ggatttacgg
     3781 cagagttcga aacgttggtg cgcaggagga atcgtgaact acgccctact cagggccttc
     3841 gggcaggcgt tccgcacgcc cgacctgcgc aaaaagatgt tgttcgtgct cttcatcgtg
     3901 ctgctgttcc gcttgggcag cgcgatcccg gcaccgggtg tgtcggagaa gaacatccac
     3961 acgtgtctcg tcgaggtcac cggcggcaac agcgcaaacg tctacaccct gatcaacctg
     4021 ttcagcggtg gcgcgctgct caaactgtcg attctggcgc tgggcatcat gccctacatc
     4081 acgtcgagca tcatcatcca gctgctcgtc gtggtgattc cgcggctcga gcggttgaag
     4141 caagagggcc agtccggtca ggcgaagctg acgcagtaca cgcgttacct gacgatcggc
     4201 ctggcgatcc ttcagtcgtc tgccttcgtg gcgcttgccc gcagccccgg tcagatcttc
     4261 accggctgca ccacgccgat cattcccaac gacggctggc aggtgatcct gaccatcgtg
     4321 ctcacgatga cggcgggcac cgcggtcatc atgtggctgg gcgagctcat caccgaccgc
     4381 ggtatcggca acggcatgtc gatcctgatc ttcacgcaga tcgcggcgcg cttcccctcc
     4441 gagctgaaca gcattcggca gaccaagggt ctcttcacgt tcttgatggt gatcctggtc
     4501 ggcgccgtca tcatcgcggc catcgtgttc gtcgagcagg cccaacgacg cattccggtg
     4561 cagtacgcga aacgcatgat cggccgccga atgtatggcg gcacatcgac gtacctgccg
     4621 ctgaaggtga accaggccgg tgtcatcccg gtcatcttcg cgtcgtccct gctgtatatc
     4681 ccttcgctct tgacccgggt ctggagcggc aacctcggct tcacccagtt cgtcacgaac
     4741 tatttgtcgc cgaacgggca tctcacttac ttgctgctgt acgcggccct gatcatcttt
     4801 ttcacgttct tctacgtcgc gatcacgttc aatcccgacg aggtcgccga gaacatgaag
     4861 aagtacggcg ggttcatccc gggcattaga cccggccgcc cgaccgccga atacctgacg
     4921 tacatcctgt cccgcatcac ggttccgggc gctacctatc tggccgtggt cgccgttatc
     4981 cctgaggcgg ccctgtcgat catcggaagc tcgcagcagt tcccatttgg tggcacctcg
     5041 gttctgatca tcgtcggcgt cggactcgac acggtgaagc agatcgagag ccagctgatg
     5101 ctgcgtaact acgag
//
LOCUS       DATCBJ010000155         6174 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_106757, whole genome shotgun sequence.
ACCESSION   DATCBJ010000155 DATCBJ010000000
VERSION     DATCBJ010000155.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 6174)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6174)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6174
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_106757"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..753)
                     /locus_tag="VII50_03185"
     CDS             complement(<1..753)
                     /locus_tag="VII50_03185"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEY5049877.1"
                     /translation="MTTVRSDTIPNDKGWLVRRSTRASRVASVISLVAVVALFAIPEV
                     MGANAVQQLTSLFTFLILAIMWNALAGYGGMVSVGQQAFIGFGAYTTIFLTQHGVTPY
                     LAVVIAAFASAALAAVESLLVLSLRGGQFAVGTWVVAEVLALLVTLDHSMGGGSGISL
                     HGLNRYSITDRRDYTYWLSLAFMVLLLGSVFLLLRRRTGAALQAIRDDEEAAASVGVR
                     VRPLRFLLFVLAGFGCGAAGALILADTSFIQPT"
     gene            complement(750..1628)
                     /locus_tag="VII50_03190"
     CDS             complement(750..1628)
                     /locus_tag="VII50_03190"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEY5049878.1"
                     /translation="MSWVNAVVQGLLLGGLYALFACGLSLMFGVMRIINLAHGDIAVL
                     GAFLVWEVAFTYHVSPFVALLPVLPIMAAFGYVLQRSVLARSLRGGPLIPLLTTFGLS
                     IVIQNGLLQTYSPDVRSLGARAGTISTKSWVLNSQIAVPYIGLLVLALAVVVLGGLQL
                     TLRRTPFGREMRATALDPDTAALVGIPAWSVYAKATAIALATATLAGAFLAIRSTFQP
                     SSGPTQLIFAFEAVVIGGLGSLWGTLGGGIVLGIAQTIGANIDPRYSILAGHLVFLAV
                     LASPRGRILASRSTTT"
     gene            complement(1625..2371)
                     /locus_tag="VII50_03195"
     CDS             complement(1625..2371)
                     /locus_tag="VII50_03195"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY5049879.1"
                     /translation="MTLLEVHDLQARHGLLQAVRGVSFGVSQGETVALVGANGAGKST
                     MLRTLAGAHPASGGSVRFDDADITNVPAHRRVALGIALVPEGRKLFADLTVEENLLVA
                     GRRAKPGPWTVDTVVAAFPMLEPLRNKKASQLSGGEQQATSIGRALMTNPRLLMVDEV
                     SLGLAPIAVDAVYESMSSLISSGSTVVLVEQDLRRAMSVADRVICILEGKIVLEAATA
                     SVSRELITEAYFGLRTPPPLEPDVSAPESA"
     gene            complement(2368..3138)
                     /locus_tag="VII50_03200"
     CDS             complement(2368..3138)
                     /locus_tag="VII50_03200"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY5049880.1"
                     /translation="MSDAPAGSDTGTSHPDQLRLVGVGLSKRFGSSQVLDNVNFEVSA
                     HEAVGIVGPNGAGKTTLLNVLSGSLAPSAGTVHLGAQDITHMRSEQRCRRGVGRAFQI
                     PRPFGAMTVLENVLVGASYGAGLSRSAAYDKSVEVLELCGLIDLANRRAESLGLLHRK
                     RLELARSLATDPQVLLLDEIGGGLTDAEAAQLVDTIRRVRARGISIVWIEHIVHLLLQ
                     VIDRLVAMDAGRVIASGKPDVVLRDAAVVDAYLGKAGP"
     gene            complement(3135..4571)
                     /locus_tag="VII50_03205"
     CDS             complement(3135..4571)
                     /locus_tag="VII50_03205"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY5049881.1"
                     /translation="MDPSSQPEVVQAAISRRNFLRAAGLGATTLAFTSPLLAACSSSK
                     KTPAATSSAATSSAASTTAAAPSASTAATTPAAAASGGPSAGTLKIGYVSPITGADAG
                     FGEPDGYVLGLARKAFANGIVIDGKTWDVQIIDKDGQSDPARAAQVANDLINSSKVDL
                     MLATSTPEQVNPVSDACEAAGVPCVSTVVPWEAWYYGRGAKPGNNDAYKFTYHFCFGV
                     QQFDNAYTTMWPQVSTNKKVGVMWPNDADGNAIRSALGPLLTKAGYTIVDPGAYTDGT
                     NDYSSQINKFKSGNCEIFNTFPIPPDFATFWQQAAQQGYKPKIAQIAKTGLFPSQVEA
                     LGSIGFGLASGVYWDPTFPYSSSLTGVSSADLATGYQTAAGKQWNQQLGASLALFDVA
                     AAALKASSNPQDKTAVSTAISTLSVMTPVGQLQWGKGPNANVVVTPIIGGQWVKAPSG
                     PYKLAFVICEHSSDTNVPIAGTLQPYGA"
     gene            4660..5529
                     /locus_tag="VII50_03210"
     CDS             4660..5529
                     /locus_tag="VII50_03210"
                     /inference="COORDINATES: protein motif:HMM:NF013757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HEY5049882.1"
                     /translation="MGYDSNMVADGAKGRQPATVSATVSATAGFSQSLERGLQILAVF
                     NESRPVLGIADIARAVDLNKSTTYRYVATLARLDYLQQDPETKKYSLGPRVADLGFAA
                     INSMEITRVAARPLQALADETGHTVSMAVLDGSDIVYVDRRRSARGVHQGMELNLHVG
                     SRLPAYCTALGKVLLAYRDPATLRMLLDRTDLARRAPKTITAREQLTFALARIREVGI
                     GVNDEELAPGLRAIAAPVRARSGEVIAAVNIVVYLSIATAPMEQLSARLEAPLRRTTT
                     DISARLGYRSESS"
     gene            5640..>6174
                     /locus_tag="VII50_03215"
     CDS             5640..>6174
                     /locus_tag="VII50_03215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009079167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEY5049883.1"
                     /translation="MFLDDSRWKGQIFVGGSWVPGAGGVVDVIEPATGATLAQSGLAG
                     PTDVAAAAISASEAQREWAALPHPARAAVLRKAGALWAEHAEEISWWNIREVGAIEGM
                     AGFAVHVAEQECYEAAALPSRPYGELLSSEGPRLSLARRLPVGVVGVISPFNVPIILG
                     IRSVAPALALGNAVILKP"
BASE COUNT         1113 a         1971 c         2067 g         1023 t
ORIGIN      
        1 cgtcggctga atgaacgacg tgtcggcaag gatgagcgca ccggcggcgc cgcaaccgaa
       61 gcccgcgagg acgaacagca ggaatcggag cggccgcacg cggacgccga ccgacgctgc
      121 agcctcctcg tcgtcgcgaa tggcctgcag agcggcgccg gttctccggc gcagcagcag
      181 gaagaccgaa ccaagtagca gcaccatgaa cgccagagac agccagtacg tgtagtcgcg
      241 gcggtcggtg atcgagtacc ggtttaggcc gtgcaacgag atgcccgaac cgccgcccat
      301 gctgtgatcg agggtgacga gcaaagcgag aacttcggca acaacccagg tcccgacggc
      361 gaactgaccg ccgcgcaaag acagcacgag taacgactca accgctgcca gtgctgcaga
      421 cgcaaacgcg gcgatgacga ccgcgaggta aggcgtcacg ccgtgttggg tcaagaagat
      481 cgtcgtgtag gcaccgaagc cgatgaacgc ttgttgcccc acggagacca ttccgccgta
      541 gccggcgagc gcgttccaca tgatggcaag gatgaggaac gtaaaaaggc tggtcagttg
      601 ctgaacggca ttcgcgccca tcacctcggg tattgcaaac agcgcgacga ccgccacgag
      661 cgatatgacg cttgccactc gggacgcgcg cgtcgaacgt cggacgagcc accctttgtc
      721 gttgggtatc gtgtcgctgc gcacggttgt cacgtcgttg tgctccggct ggcgaggatt
      781 cgtcctcgag ggctcgcaag gacggcgagg aagaccaggt ggccagccag gatcgagtac
      841 cgcgggtcga tgttggctcc gatcgtttgc gcgatgccga gcacgatgcc gccgcccaag
      901 gtcccccaca gtgagccgag tccgccgatc acgacggcct cgaacgcgaa gatcagctgc
      961 gtggggccgc tcgagggttg gaaggtcgac ctgatcgcga ggaatgcgcc tgccaaggtg
     1021 gcggtcgcga gcgcgatcgc ggtggccttg gcgtacacag accaggcagg gatgccgacg
     1081 agcgccgcgg tgtccgggtc gagcgcggtt gcccgcatct cccgcccaaa cggcgtgcgg
     1141 cgcagtgtga gttggagacc gccgagcacg accacggcga gtgcgagcac gagaaggcct
     1201 atatagggaa ccgcaatttg ggagttgagg acccagcttt tcgtcgagat cgtgccggcc
     1261 ctggcaccga gcgagcgaac atccggtgag taggtctgca gcaaaccgtt ttggataacg
     1321 atcgacaaac caaatgtcgt cagcagcggg atcaagggtc caccgcgaag gctgcgcgcc
     1381 agcacgctgc gctgcaggac gtagccgaag gccgccatga tcggcagcac cggcagcagt
     1441 gcgacgaacg gcgagacgtg gtaggtgaat gcgacctccc acacgaggaa ggcgccgagc
     1501 accgcgatgt caccgtgcgc gaggttgatg atccgcatca ccccgaacat gagtgacaag
     1561 ccgcaggcga acaaggcgta gaggccgccg agcaggaggc cctgaacgac ggcgttgacc
     1621 caactcatgc cgactccggt gcagagacgt cgggctcaag cggaggcggt gttcggaggc
     1681 caaagtatgc ctcggtgatg agctcgcgac tgaccgacgc ggtcgcggcc tcgagcacga
     1741 tcttgccttc caggatgcaa atcacccggt cggcgaccga cattgcacgg cgcagatcct
     1801 gttcgaccag cacgacggtg gagcccgaac tgatgagtga cgacatcgac tcgtaaaccg
     1861 cgtcgaccgc gatcggcgcg agaccaagcg atacctcgtc gaccatcagc aggcgcgggt
     1921 tggtcatgag cgctcgacca atagacgttg cctgttgctc cccgccagac agttgtgacg
     1981 ccttcttgtt gcgaagcggc tcgagcatgg ggaaagcagc caccacggtg tcgacggtcc
     2041 atgggccagg ctttgcgcgc cggccggcga cgagcaggtt ctcctcgacc gtgagatcag
     2101 caaacagctt gcgtccctcg ggtacgaggg cgattccgag cgcgacccgc cgatgcgcgg
     2161 gcacattggt gatgtcagca tcatcgaagc gcactgatcc gccggaggct ggatgtgcgc
     2221 cagcgagcgt ccggagcatg gtggacttgc cggcgccgtt cgcgccgacc agggcgacag
     2281 tttcgccctg gctcacaccg aagctgaccc cgcgcacggc ctgcaggagt ccgtgacgag
     2341 cttgcaaatc atggacctcc agcagggtca cgggcctgcc ttccccagat acgcgtcgac
     2401 gacggcggca tcgcgaagca ccacgtccgg cttgccgctg gcgatcaccc tcccggcgtc
     2461 catcgcgacg agccgatcga tgacctgaag cagcagatgc acgatgtgct cgatccacac
     2521 gatgctgatc ccgcgggctc ttacccgccg gatcgtatcg accaattggg cagcctcggc
     2581 gtcggtcagc ccgccgccga tctcgtcgag caacaacacc tgcggatctg tcgccaacga
     2641 ccgcgccagc tccagtcgct tgcgatgcag cagacccagg ctctccgcgc gccggttggc
     2701 caggtcgatc agcccgcaca gttccagcac ctcgacagat ttgtcatagg cggcggacct
     2761 gctgaggccg gcgccgtagg acgcacccac gaggacgttc tcgaggaccg tcattgcgcc
     2821 gaagggacgc gggatctgaa acgcccgccc cacaccacgg cggcagcgct gctcggatcg
     2881 catgtgggtg atgtcttgcg cgcccagatg cactgttccg gcactcggcg cgagtgagcc
     2941 ggaaagcacg ttcagcaaag tggtcttgcc ggccccgttc gggccgacga tcccgaccgc
     3001 ttcgtgcgcg ctgacctcga agttgacatt gtcgagcact tgcgatgagc cgaaccgttt
     3061 ggacagtccc accccgacca gccgaagctg atcggggtgt gatgtcccgg tgtctgaacc
     3121 ggccggtgcg tcgctcacgc gccgtaaggc tgcaacgtgc ctgcgatggg gacgttcgtg
     3181 tcggaggagt gctcgcaaat gacgaacgcg agcttgtacg ggccggacgg cgccttgacc
     3241 cactgaccgc cgatgatcgg cgtgaccacg acgttggcgt tgggcccctt cccccactgc
     3301 agttgcccga ccggggtcat gacactgagc gtgctgattg ccgtcgacac cgccgttttg
     3361 tcctggggat tcgatgacgc cttcagcgct gctgccgcca cgtcgaatag cgcaaggctg
     3421 gcacccaatt gctgattcca ctgctttccg gcggcggttt gatagccagt tgcgagatcg
     3481 gccgagctga cgccggtaag cgacgacgag tagggaaacg tcggatccca gtacacgccg
     3541 ctggcgagcc caaacccaat cgagcccaac gcctcgacct gggacgggaa gagtccggtc
     3601 ttggcgatct gcgcgatctt cggcttgtag ccctgctgcg cagcctgctg ccagaacgtc
     3661 gcgaagtccg gcgggatggg gaaggtgttg aagatttcgc agttgccgga cttgaacttg
     3721 ttgatctgcg aggagtagtc gttcgtgcca tcggtgtagg caccggggtc aacgatcgtg
     3781 tacccggctt tcgtcaacag cgggcctagt gcggatcgga tggcgttccc atcggcgtcg
     3841 ttgggccaca tgacgccgac cttcttgttc gtcgacacct gtggccacat cgtggtgtag
     3901 gcattgtcga actgctggac accgaagcag aagtgatacg tgaacttgta cgcgtcgttg
     3961 tttccgggct ttgcgccccg accgtaatac cacgcctccc agggcacaac ggtcgacaca
     4021 cacggcacgc cggccgcttc gcacgcgtca gagaccgggt tgacctgctc gggggtcgac
     4081 gtggcgagca tcaggtcgac cttcgagctg ttgatcaggt cgtttgccac ctgagcggcg
     4141 cgcgccgggt cggactgccc gtccttgtcg atgatctgga catcccaggt cttgccatcg
     4201 atcacgattc cgttagcgaa tgccttgcgg gcaaggccga gcacgtagcc gtcaggctca
     4261 ccgaatccgg cgtccgcgcc cgtgatcggg ctgacgtagc cgatctttaa cgtccccgcg
     4321 ctggggccgc ccgaggctgc tgcggctggc gttgtcgccg cggtggacgc agagggagcg
     4381 gccgccgtcg tggacgcggc gctcgacgtc gctgcgctgg acgtcgcggc gggtgtcttc
     4441 ttgctgctcg agcaagcggc gaggagcggg cttgtgaacg cgagcgtcgt cgcgcccaag
     4501 ccagcggcgc gtaagaaatt ccgccggctg atggccgcct ggacgacctc gggctgggaa
     4561 gaagggtcca ccatgacctc cacgattgcg ggtgagtggc ccgaaacgta ggaggcggcg
     4621 tgctggccgt caacgcctgg tttcactagg tggaacactt tgggttacga ttcgaacatg
     4681 gtggccgacg gcgcgaaagg gcggcagccg gcgaccgtca gtgcgaccgt cagtgcgacc
     4741 gccggatttt cgcagtccct cgagcgtgga ctccaaatcc tggccgtgtt caacgagtcg
     4801 cgaccggtgc tgggaattgc cgacatcgcg cgtgccgtcg acctgaacaa gagcacgacc
     4861 taccgctacg tagcgacgct cgcccgcttg gactacctcc agcaggaccc cgagacgaag
     4921 aagtactcgc tgggaccgcg ggtggccgat ctcgggttcg ccgcgatcaa ctcgatggag
     4981 atcacccggg tagcggcgag gccccttcag gcgctggccg acgagaccgg gcataccgtc
     5041 agcatggccg tgctggacgg ctccgacatc gtctacgtcg accggcgccg tagtgctcgc
     5101 ggcgttcatc agggcatgga gctgaacctg cacgtcggct cgcggctgcc tgcgtactgc
     5161 actgcactcg gcaaggtcct gctggcttat cgcgacccgg ccacgctgcg gatgctcctc
     5221 gatcgcacgg atctggcccg ccgtgcaccg aagaccatca cggcccgcga gcagctgacg
     5281 ttcgccctcg cgcggatccg cgaggttggc atcggggtca acgacgagga actggcccca
     5341 gggctgcgcg cgatcgccgc gccggtacgc gctcgatccg gtgaggtcat cgccgccgtc
     5401 aacatcgtcg tctacctgag catcgccacc gccccgatgg agcagctgag cgcccggctt
     5461 gaggcgccgc tgcggcggac caccactgac atatccgccc gactcggcta tcggagcgag
     5521 tcgtcctagc tagtgaaccg cgcgtttcaa ctatccgaac actcggttga ttattcagcg
     5581 tggcccgtct acggtctggg cctcagccct gtcatcacgt cgcgcatagg aggcaacggg
     5641 tgttcctgga cgactcgagg tggaagggcc agatcttcgt cggcgggtcc tgggtgcccg
     5701 gcgccggcgg tgtagtcgac gtgatcgagc ccgcgacggg agcgacgctg gcccagagcg
     5761 gtcttgccgg tccaaccgac gtcgcggctg cggcgatcag cgcgagtgaa gcgcagcgcg
     5821 agtgggccgc gctgccgcac ccggcgcgcg cggccgtcct gcgcaaggcc ggcgcgttgt
     5881 gggccgagca tgccgaggag atcagctggt ggaacatccg agaagtcggt gccatcgaag
     5941 gcatggcggg cttcgcggtg catgtcgcgg agcaggagtg ttacgaggcg gctgctctcc
     6001 cgagtcggcc ttacggggaa ctcctctcca gtgaaggccc gcggctgtcg ctcgctcgtc
     6061 ggctgccggt cggcgtcgtc ggcgtcatct cgcctttcaa cgtcccgatc attcttggca
     6121 tcaggtcggt ggcgcccgca ctggcgcttg gaaatgcggt cattcttaag cccg
//
LOCUS       DATCBJ010000156         3215 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_131665, whole genome shotgun sequence.
ACCESSION   DATCBJ010000156 DATCBJ010000000
VERSION     DATCBJ010000156.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 3215)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3215)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3215
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_131665"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..189
                     /locus_tag="VII50_03220"
     CDS             <1..189
                     /locus_tag="VII50_03220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011689782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HEY5049884.1"
                     /translation="MKANVAIQHSMLIAIWHIARTGALYDDPGADYFTRLHPERAKNR
                     ALHQLEAMGYQVTLEQAG"
     gene            complement(237..1232)
                     /gene="sepH"
                     /locus_tag="VII50_03225"
     CDS             complement(237..1232)
                     /gene="sepH"
                     /locus_tag="VII50_03225"
                     /inference="COORDINATES: protein motif:HMM:NF040712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septation protein SepH"
                     /protein_id="HEY5049885.1"
                     /translation="MAQLRLVGVSEDGERLVLAGDDGVQHEIVIDERLRAGVHGHLSR
                     LGQLTMALESRISPREIQNRVRSGESAEHVAQTAGVPLERVLRFVGPVLREREHIADQ
                     ARRARLAGSSGPASLLGDLVSRAASRRGVDADDVDWDSARREDHTWVVTTAWPDGTTA
                     MWALDLLRHQATPLDTAARRISGSVDEATDESTAKSRGFPDIANSPDFARFDANQTDA
                     VRLETGPDSDTGAKVHQFIPRSVPTAVEDLLPDDTAVEDVVEDIEADDEVLAEVDEPS
                     AVDTSVDNDDAVASTEPTAEPAAGTPRRQGGRAVRRAAVPKWDDIMFGMKPKDGS"
     gene            1440..2039
                     /locus_tag="VII50_03230"
     CDS             1440..2039
                     /locus_tag="VII50_03230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5049886.1"
                     /translation="MTDGQPAPPRADLPVASNLVVAVQPRRGGDVLEVSMATGGVLGH
                     FDPLMAQLFVIDPQDSLAVAAAVAPYLLGDEQPLGVLSPRHIAAAETLWGILESSPFT
                     WQRFVPLHDRLLFFYCPLAHLAVEIVDDGEAASDVEAPRFAQLAVSLATIPLRDLEHR
                     PGAVASWLSALCTRRADSRPGASPDADMHRSMWMRLTHG"
     gene            complement(2055..2636)
                     /locus_tag="VII50_03235"
     CDS             complement(2055..2636)
                     /locus_tag="VII50_03235"
                     /inference="COORDINATES: protein motif:HMM:NF012624.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside monophosphate kinase"
                     /protein_id="HEY5049887.1"
                     /translation="MHVLLIGPPASGKGTQGRRIAARFAVTYLSSGEILRKEVASATA
                     LGHRVADDLAAGDLVPDDLMLEVLRTPLEAALTSGGYVLDGFPRNVAQALELAAYAAQ
                     LGGIPDAAVWFDVEDAELLRRTRSRAIAEGRVDDVDAVARHRIDVYAAATAPLIDHYR
                     AQHVLIRIDAAKPVEEVTETLLAALEPFATRPS"
     gene            2685..>3215
                     /locus_tag="VII50_03240"
     CDS             2685..>3215
                     /locus_tag="VII50_03240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - ferrochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0006783 - heme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HEY5049888.1"
                     /translation="MRPYDAVLLVSFGGPEGPDDVMPFLENVTAGRGVPRERLVDVAQ
                     HYYRMGGVSPINGQNRALLAALRDEFEEHGVGLPLYWGNRHWAPTLPDVLAQMRDDGI
                     TNAIAFLTSAYASYSSCRQYLDAIERARAEVGDGAPAVDRLRQYFNHPGFIEPMIRGV
                     EAALQLLPAEYRDDAPL"
BASE COUNT          519 a         1082 c         1054 g          560 t
ORIGIN      
        1 atgaaagcaa acgtcgcgat ccaacactca atgctcatag cgatctggca catagcccga
       61 accggcgccc tctacgacga ccccggcgcc gactacttca ccagactgca ccccgaacga
      121 gccaagaacc gggcactcca ccaactcgaa gcgatgggct accaggtcac cctcgagcaa
      181 gccggataag ccgaacaacc cgcgccggtc accaagggaa tcttcgcgtc agaggcctag
      241 gagccgtctt tcggcttcat gccgaacatg atgtcgtccc acttcggcac cgcggcccgt
      301 cgcactgcgc gcccgccctg tcgtcgtggg gtcccagccg caggttcggc ggtcggctcg
      361 gttgacgcga ctgcgtcatc gttgtcgacc gacgtatcaa ctgcggacgg ctcgtcgacc
      421 tcggccaaca cctcgtcgtc cgcctcgatg tcttcgacaa cgtcctcgac ggctgtgtca
      481 tcgggcagca ggtcttcgac cgcggttggc actgaccgcg ggatgaactg gtgcaccttc
      541 gcgccggtgt cggagtcggg gcccgtctcc aggcgcacgg cgtcggtctg gttcgcatcg
      601 aaccgcgcga aatcggggga gttcgcgatg tcggggaagc cgcgcgattt agcggtggac
      661 tcatctgttg cttcgtcgac ggacccgctg attcggcggg cggcggtgtc gagcggggtg
      721 gcctggtggc gcagcaggtc gagtgcccac atcgctgtcg tgccgtctgg ccaggccgtg
      781 gtgacgaccc aggtgtggtc ctcgcgtcgc gcggaatccc agtcgacgtc gtctgcgtcg
      841 acgccccgcc ggctcgctgc tctgctcacc aggtcgccaa gcagtgacgc cggaccgctg
      901 ctgccggcca gtcgcgcgcg ccgggcttgg tcggcgatgt gctcgcgttc acgaagcacc
      961 ggcccgacga acctcaagac gcgttcgagc ggtacgcccg ccgtctgtgc gacgtgctcg
     1021 gccgactcac cgctacgcac ccggttctgg atctcgcgcg gggagattcg gctctcgagc
     1081 gccatggtca gctgccccag tcgcgagaga tggccgtgca ctcccgctcg aagccgttcg
     1141 tcgatgacga tctcgtgctg gacgccgtcg tcgccggcca gaaccaggcg ctcgccgtcc
     1201 tcgctcacgc cgaccagccg cagctgcgcc atctgacctc ctcaccgcct accgacgcgt
     1261 ccctcgtgct gactcaaatg tccgagggca gtctgccgcg ccgaccggtc cttgggccaa
     1321 tcgggtagcg attgcagacg ttcgtggcaa cggcctgaac ccgaacgggc gagcggtggc
     1381 gtgcgcggcc ggtcgaagga tcagtacgta caagggcaaa ttcacatgga ggaggcgacg
     1441 tgacggacgg tcaaccggca ccgccgcgcg ccgacctgcc cgtggcgagc aatcttgtcg
     1501 tcgccgtgca gccacgccgc ggcggagacg tgctcgaggt gagcatggcg accgggggag
     1561 tgctcgggca cttcgatccg ctgatggctc agctgttcgt aatcgatccg caggacagcc
     1621 tcgctgtcgc cgcggcggtt gcgccgtatc tgctcggcga cgagcaacca ctcggtgtct
     1681 tgtcgcccag gcatatcgct gctgcagaaa cgctgtgggg aatcctcgaa tcgagtccct
     1741 ttacctggca gcgctttgtt ccgctgcacg atcggctgct gttcttctac tgtccactcg
     1801 cgcacctcgc cgtggagatc gtcgacgatg gcgaggccgc gtctgatgtc gaagcgccga
     1861 ggttcgcaca gctcgcggtc agcctcgcga caatcccgtt gcgcgatctc gaacaccgcc
     1921 ccggcgcagt tgccagctgg ctcagtgctt tatgcacgcg gcgtgcggat agtaggccag
     1981 gtgcatcgcc ggacgccgac atgcaccggt ccatgtggat gcgcctgacg cacggctgac
     2041 cgaatgccgc gacgctagct tggcctggtt gcgaacggct caagtgcggc gagcagggtc
     2101 tcagtgactt cttcgaccgg cttggcggcg tcgatgcgga tcagcacgtg ctgtgcgcgg
     2161 tagtggtcga tgagcggcgc ggtggccgct gcgtagacgt cgatgcgatg ccgggcgacc
     2221 gcatcgacat cgtcaacgcg accctccgca atcgctcggc tgcgggtgcg ccgaagcaat
     2281 tccgcgtcct cgacgtcgaa ccacacagcc gcgtcaggga tgccacccaa ctgggcggcg
     2341 taggccgcaa gttcgagagc ctgcgcgaca ttgcgaggga atccgtcgag cacgtatccg
     2401 ccactggtca gcgcggcttc caagggcgtt cgcagtacct cgagcatgag gtcgtcaggc
     2461 accagatccc cggccgccag atcatcggca acccggtgtc ccagtgctgt ggcggacgcg
     2521 acctccttgc gcaggatctc gccggaggac aggtaggtga cggcgaatcg ggcggcgata
     2581 cggcgacctt gggtgccctt gcccgaagcc ggtgggccga tcagcaggac gtgcacagtg
     2641 gagcctttcg tcggtcagct gtcggcagct gcgaagatcg aaagatgcga ccctatgacg
     2701 ccgtgctcct cgtctcattc ggtggtcctg aagggcccga cgacgtgatg ccgtttctgg
     2761 agaatgtgac cgccggtcgt ggggtgcccc gggagcggtt ggtcgacgtt gcccagcact
     2821 actaccgcat gggcggagtg agcccgatca acggccagaa ccgtgctctc cttgccgcac
     2881 tacgcgacga gttcgaggag cacggggtcg ggctgccttt gtattgggga aaccggcact
     2941 gggcgccgac actgcccgac gtactcgccc agatgcgcga cgacggcatc acaaacgcca
     3001 tcgcgttctt gacgtctgcg tacgcgagct attccagctg ccgtcagtac ctcgacgcca
     3061 tcgagcgggc gcgcgccgaa gtcggcgatg gcgcgccggc tgtcgatcgg ctccggcagt
     3121 acttcaacca tcccggcttc atcgagccca tgatccgtgg cgtcgaagcg gcgttgcagc
     3181 tgctccctgc cgagtaccgc gacgacgcac ccctc
//
LOCUS       DATCBJ010000157         3303 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_96645, whole genome shotgun sequence.
ACCESSION   DATCBJ010000157 DATCBJ010000000
VERSION     DATCBJ010000157.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 3303)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3303)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3303
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_96645"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2786)
                     /locus_tag="VII50_03245"
     CDS             complement(<1..2786)
                     /locus_tag="VII50_03245"
                     /inference="COORDINATES: protein motif:HMM:NF027952.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HEY5049889.1"
                     /translation="MRQRMRTTGGGSPGGDAVRGPRRRRRRQRILVVAATTAGMLVFP
                     LRSAVGAVSNTVVLTGGPSGQPNDVRLIAADSAATVFSVTDPELAAGPTTYVQPHNGA
                     ARPLPARFTLSGVAAPTLVDDMLGAYEPASASGVGSFIYGTIDGSVSGGAPVTAGEFQ
                     GTSADGYLYSAVPDASAGAGGSVHLYDVDVTTKATTDLGAVPGNPAFLTATASAAGVL
                     LETKETGDSSEQPSLDYVRYADPSTFVQLASSVPIQGGPVLGTNSVAWLENATVYDSA
                     QAFDPTATKVLRVTLGGSVVERRAAPNAYEVATTPTYTGVLTASPNEAAASFSTLATH
                     GGQQTAYQLPVGADLLSTDTDFVVTEGGTPGTAGIYTLPSAAGPAVLLHPAGAATLRA
                     STIAVSPARVVWRDNGIGAGLWSRALGTVQTTLAAGDPSIVSSQPDSTSEESVSSSGD
                     RVAFVGSGNTTSTNTTSTLSLVAPGQSPRIIGPAAPDDELTLSGTRLLQRHDDGSAGL
                     IDLVSGATSNLPAPVAPWSGAFLGRTTYQLWGNELAWLASDGSVWFKDLAADTTTEVS
                     GPAVGAGESVVGSVAVAAGIVAWSESICKPTGAAIAGVSTCSGTGLSYRNALTLGQTY
                     TVQSARPTRIQLSNGYIAFDDNSNGSSWVDVAPLYSAIVEHIAALSSGGAGEAEFSIS
                     GSMLGWIGADNLPHAAALPHIAAKPWYLGNGVVATSLVTDGTHNWEGDFFTSEGLTQC
                     AVTISTGSTKVRLLPCDTTHMIVGEAVVSWDGKDAVGSTVSPGTYSWTLTGAGVDGPI
                     LNADGSDTPISGTISTTPHSIVSLACASSSGAAYHPVTLTASVAGSSPGTIEFFDGAT
                     SLGSSPVSGGVATLTTDKLGPGSHSMTATFTAGDRGPPATSAPVALMLSESSSHVGVV
                     VQPSVDPGTLTMTTP"
BASE COUNT          589 a         1047 c         1084 g          583 t
ORIGIN      
        1 ggcgtcgtca tggtcagcgt gccgggatca accgacggtt gtacgaccac gcccacatgt
       61 gacgacgact cactcaacat cagcgctact ggcgccgagg tagcaggagg tccgcggtcg
      121 ccggccgtaa acgtagcggt catcgaatgc gatccagggc caagcttgtc ggtggtcagc
      181 gtcgcgacgc cgccagacac cgggctcgag ccaagagaag tggcgccgtc gaagaactcg
      241 atggtgcctg gacttgaacc cgcgacgctc gctgtgagcg tgactgggtg gtaggcggcg
      301 ccgctgcttg atgcgcatgc gagcgacacg atcgaatgcg gcgtcgtact gatcgttccg
      361 ctgatcggcg tgtcgctacc gtcggcattg aggattggtc cgtcgacacc ggccccggtg
      421 agagtccagc tgtaggtgcc cggcgagacc gtcgagccaa cagcatcctt cccgtcccag
      481 ctgacgaccg cctcgcctac gatcatgtgc gtcgtgtcgc agggcagcaa gcggactttg
      541 gtgctgcccg tagaaatagt tactgcacac tgcgtaagtc cctcggatgt gaagaagtca
      601 ccctcccagt tgtgtgtgcc gtcggtgacg agtgacgtcg ccactacgcc gttcccgagg
      661 taccacggct tggccgcgat gtgtggcaag gctgctgcat gcggcaggtt gtccgccccg
      721 atccaaccaa gcatcgatcc tgagattgag aactcggcct caccggcgcc gccggatgag
      781 agcgctgcga tgtgttcgac tatcgcggaa tacagcggtg cgacgtcgac ccacgaagaa
      841 ccgttggagt tatcgtcaaa tgcgatgtag ccattcgaga gttgaatccg cgtcggtcgc
      901 gccgactgca cggtatacgt ctgtccaagt gtgagggcgt tgcggtaact caagcccgtg
      961 ccgctacacg tgctcacgcc ggcaatcgct gcaccggtag gtttgcaaat gctctcgctc
     1021 cacgcgacga tgccagccgc cacagcgaca ctgccgacaa ccgactcacc cgcgccgact
     1081 gctgggcccg agacctcagt cgtggtgtcc gccgcgagat ccttaaacca taccgaccca
     1141 tcggaggcga gccaggcgag ctcgtttccc cagagctgat acgtggtgcg accaagaaat
     1201 gcgccactcc acggagcgac cggcgcaggc aagtttgagg tagctccgct cacgaggtcg
     1261 atcaacccgg cggagccgtc atcgtgccgt tgaaggagcc gagtccccga cagcgtgagc
     1321 tcatcatcgg gcgccgccgg cccgatgatt cggggagact gccccggcgc aacgagcgac
     1381 agcgtgcttg ttgtgttggt gctggtggtg ttgccactcc ccacaaaggc cacgcggtcc
     1441 ccggacgacg atacggattc ctccgacgtc gaatcgggtt gcgatgaaac gatactggga
     1501 tcaccagccg cgagcgtggt ctgcacagtt cccagcgcgc ggctccacaa gcccgcgccg
     1561 ataccgttgt ctcgccacac gacacgcgct ggcgaaacag cgatggtgct tgcgcgcaag
     1621 gtggctgcac cagctgggtg cagtaggacg gccggccctg cggccgatgg caaggtgtag
     1681 atgccggccg tgcccggcgt gcccccctca gtgacgacga agtccgtgtc ggtcgacaac
     1741 aggtcggcgc caacgggcag ttggtacgca gtctgttggc cgccatgcgt tgccaatgtc
     1801 gagaatgagg cggcagcttc gttgggtgac gctgtgagta cgccggtata tgtaggagtc
     1861 gttgccacct cgtaggcatt cggagccgcg cggcgctcaa cgacagaccc gcccaacgtc
     1921 acacgcagga ccttcgtcgc cgttgggtcg aacgcttgag cgctgtcgta gacggtcgca
     1981 ttctccagcc aggcgacgga gttcgtgcca agaaccggac caccctgaat cggtacgctc
     2041 gatgcgagct gaacgaaggt gctcgggtcg gcgtagcgga cgtagtccaa ggacggttgc
     2101 tcggaactat ccccggtctc tttcgtctcg aggagaacac cagccgccga ggcggtcgcg
     2161 gtcagaaagg ccggattgcc cggtacggcg ccgaggtccg ttgttgcctt cgtcgtgacg
     2221 tcgacgtcgt agaggtgaac gctgcctccc gcaccggcgc tggcgtcagg tactgccgaa
     2281 tatagatagc cgtctgcgct cgtgccctgg aactctcccg cggtcacagg tgcgccgccg
     2341 ctaaccgagc cgtcgattgt gccgtagatg aagctgccaa cccccgacgc cgatgcgggc
     2401 tcgtaggctc cgagcatgtc gtcaaccagc gttggcgcag caaccccact gagagtgaag
     2461 cgggcgggca gcgggcgagc agccccgtta tgcggctgca cgtacgtggt cgggccagct
     2521 gcgagttccg ggtcggtcac cgagaacact gtcgcagcgg aatccgctgc gatgaggcgg
     2581 acgtcgttgg gttgcccaga agggccgccg gtcagtacca cggtattcga cacggcgccg
     2641 acagccgagc gtaagggaaa tacgagcatg cctgcggtag ttgcagcgac gacaaggatc
     2701 cgctggcggc gacgccgtcg tctaggacca cgcacggcat caccgcctgg cgatccaccg
     2761 cctgtggtgc gcatgcgctg cctcacagcg agtccttggt tctctctgct cgacgtggac
     2821 gagactcggc aggcttcgcc gattatggcc tgacgggctc gcgcgccaag gggtccagac
     2881 gaactgttca acgacagtta gcttgcggtt cgtctggcgg cgtactccgc actcggagcg
     2941 gcggcgccgg ttgttaacac agtggatgtt cgttgttggc tgtgccgcaa gcatccgtta
     3001 gctgcgttcc tccactgtca tggcgtggtt gagatcgcac cttccggcgc gcacgctcgg
     3061 gtggcacaac acgctcgggt ggcacaacac gcgagggttc cacggcacgt cggggccccg
     3121 caaattcttc gggcgccgga gccagttcag cctgttccgg gaccaccgaa cattcgagta
     3181 gcgcggcacg tcagggccgc aagacacgcg gtccgcccag tcgaggctcg ccacgccggt
     3241 gctcgacgca ctctgccgcg cgcgcaggcg tggggcatca gtgccgcgat ttccttcttg
     3301 gcg
//
LOCUS       DATCBJ010000158         2231 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_59030, whole genome shotgun sequence.
ACCESSION   DATCBJ010000158 DATCBJ010000000
VERSION     DATCBJ010000158.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2231)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2231)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2231
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_59030"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(106..813)
                     /locus_tag="VII50_03250"
     CDS             complement(106..813)
                     /locus_tag="VII50_03250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEY5049890.1"
                     /translation="MEPSSATTATTRLSPGDPIRVLVVDDHALFRRGLEMVLAQEPDI
                     ELCGEASDGAEAVAKAAETLPDIVLMDVRMPKRSGIEACTAIKDVVPSAKIIMLTISD
                     EEADLYDAIKAGAMGYLLKEISIDEVAAAIRAVHGGQSLISPSMASKLLSEFASMIKK
                     GDSRQQVPAPKLTDREMEVLRLVAKGLNNRDIAKQLFISENTVKNHVRNILEKLQLHS
                     RMEAVVYAVREKLLEIT"
     gene            complement(997..1671)
                     /gene="raiA"
                     /locus_tag="VII50_03255"
     CDS             complement(997..1671)
                     /gene="raiA"
                     /locus_tag="VII50_03255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009082993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HEY5049891.1"
                     /translation="MEIVVTGRHLDVPALLRTHAQDRLAKVAKLAPKAQRVDVEVSRE
                     RNPRMSEICERVELTCISPGPVIRAEASAHEMLEAFDLAWAKLESRLRKAADRRRVHR
                     GTRTPVSVAAATKELGRRANGAARGSAQLGAEAADLATAANDDAAGDDADGDRPGPML
                     VREKLHKALPMTLDQALYEMELVGHPFFLFVDQQSALPSVVYRRHGYDYGVIRIDTTS
                     DSAVSR"
     gene            complement(2034..>2231)
                     /locus_tag="VII50_03260"
     CDS             complement(2034..>2231)
                     /locus_tag="VII50_03260"
                     /inference="COORDINATES: protein motif:HMM:NF012383.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HEY5049892.1"
                     /translation="RRPALVRGRRVVIVDDIVTTGATAAEAARALTLAGAVVTGIGWV
                     AGTVRRHPPASADLRGAAVAV"
BASE COUNT          344 a          788 c          694 g          405 t
ORIGIN      
        1 ctttgcacga ggccgcgacc tgggtcggat gcagcaatgt cgcagtcgag cgagcgctgc
       61 cggcttcgtc gacgactgcc attgccgcag cggtcgcggc cggccttagg tgatctcgag
      121 cagcttctcg cgcactgcgt agacgactgc ttccatccgc gagtgcagct gcagcttctc
      181 caagatgttg cgcacgtggt tcttcaccgt gttctcgctg atgaacaact gcttggcgat
      241 gtcgcggttg ttcaggccct tggccaccag ccgcagcact tccatctcac ggtccgtgag
      301 cttgggcgcc ggtacttgct ggcgggaatc gcccttcttg atcatcgagg cgaactcgct
      361 caacagcttg gaggccatcg acgggctgat taacgactgc cccccatgca ccgcgcggat
      421 cgcggcggcg acctcgtcga tcgagatctc cttgagcagg tagcccatcg cgcccgcttt
      481 gatcgcgtcg tagaggtcgg cttcctcgtc gctgatcgtg agcatgatga tcttggcgga
      541 cggaacgacg tccttgatcg cggtgcatgc ctcgatgccg ctgcgcttgg gcatccgcac
      601 gtccatcaag acaatgtcgg gcagcgtctc ggccgccttc gccaccgctt cggcgccgtc
      661 actggcttcg ccgcacagtt cgatgtccgg ttcttgcgcg agcaccatct caaggccgcg
      721 ccgaaacagc gcatggtcat cgaccaccag cacccgaatc gggtcgccag gcgaaagcct
      781 tgtggtggct gtcgtcgcgc tcgacggctc catgccgaag gcccgcccgt cgcgcggtgg
      841 caactcaccg cggccgacga gtgggcgtac ctcagccatg ccgtcgtgcc cgtcgctcaa
      901 ggtgcctccc cggtagcccg cgcatcggcg atctcgcact tgcaggcctc acggggccag
      961 cccccgccag acatggtacg gacacctgtc gcttgatcat ctgctcaccg ccgagtcgga
     1021 agtcgtgtcg attcgaatca cgccgtagtc atacccgtgc cttcggtaga ccacgctcgg
     1081 cagcgcgctc tgttggtcga cgaagaggaa gaacgggtgc ccgacgagct ccatttcata
     1141 gagcgcttga tcaagggtca tcggcaacgc cttgtgcaac ttctctcgta cgagcatcgg
     1201 accgggacga tcaccgtcag cgtcatcgcc tgccgcgtcg tcgttggcag ccgtggcgag
     1261 gtcggccgcc tcggcgccca gctgcgcact accgcgcgca gctccgttcg cgcggcggcc
     1321 aagctccttt gtggcagccg ccaccgacac cggtgtccgg gtgccccgat gcacgcggcg
     1381 acggtccgcc gccttgcgca gccttgactc gagctttgcc cacgctagat cgaaggcctc
     1441 aagcatctcg tgcgcggaag cctccgcgcg gatgaccggc cccggcgaga tgcaggtcag
     1501 ctcgacccgc tcgcagatct ccgacatccg cggattgcgc tcacgagaca cctcgacgtc
     1561 gactcgctgg gccttcggcg ccagcttcgc caccttggcg agcctgtcct gtgcatgcgt
     1621 acggagtaga gccggcacgt cgagatgccg gccggtgacc acgatttcca ccgcgggtcc
     1681 tccctctgca cgtgtaggtc cctggcgagg ccgcccggtt tgagggctgt cgtcaggttc
     1741 cttccccgta tttggcggtg tgacgcccgc ccggccgacc tggtcttcga ccccacattc
     1801 gcctgctgag gtgctcgcgg cgcgcagttg cgcatcggtg ccactactga ccttctctcg
     1861 gtcggcagcc atgtggaccg ccggcaaggc aggacttact tctaagccgc accgtgatcg
     1921 gacgacgaca agtcgcctta cgtggaccgt tttgcctgcg actggcatcg gctaaccggc
     1981 agaggttccc accgacgcaa ccacggaccc gggcggacgc cacgccaacc aacctagacc
     2041 gcgacagccg cgccgcgcag gtcggcggac gcaggtggat gccttcgcac ggtgccggcg
     2101 acccagccga taccggtcac gaccgcaccc gcgagggtca atgcccgagc tgcctcggcg
     2161 gcggtggcgc cggtggtcac gatgtcatcg acgatcacca cccggcgtcc ccgcacgaga
     2221 gcgggccgcc g
//
LOCUS       DATCBJ010000159         2005 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_208522, whole genome shotgun sequence.
ACCESSION   DATCBJ010000159 DATCBJ010000000
VERSION     DATCBJ010000159.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2005)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2005)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2005
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_208522"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            173..1144
                     /locus_tag="VII50_03265"
     CDS             173..1144
                     /locus_tag="VII50_03265"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: protein motif:HMM:NF004771.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine dehydratase"
                     /protein_id="HEY5049893.1"
                     /translation="MSFTADELAYATELVGRRVPPTPQYAWPLLGAQVGADVWVKHEN
                     HTPTGAFKVRGGLVYADRLRARRPQVSGIVSATRGNHGQSLAFAGRANGLAVTIVVPH
                     GNSPDKNAAMRGFGAEVLEHGHDYQAAREYSMELAAERELDPVPPYHPDLVLGVATYA
                     RELFDAVGELDTVYVGVGMATGISGLIGVRDLLGLRTEIVGVVAEMAPATALSFAAGK
                     QVTTESAHTFIDGVACRVPDASAIDVINAGAARILQVSEDSCADAVRLLLSTTHNIAE
                     PAGAIALAGLLSERTSARGRRVAVVQSGGNIDSSMLAEILAGRTPVV"
     gene            complement(1141..1818)
                     /locus_tag="VII50_03270"
     CDS             complement(1141..1818)
                     /locus_tag="VII50_03270"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HEY5049894.1"
                     /translation="MTTPRFVPRVDARPEDVAWPHLQWPPPKDIVLRGRCIELTLADP
                     HRDAGELFAALDHGAVWQHVAGRPIDVAGFTEALEQAASAGRWPWVVRLTTDQAGLPA
                     GAVVGTSSYLEISPRDARLEIGFTAYTPAVWAGVVNPETKLLLLGYAFETLNAGRVQL
                     KTDVRNARSQQAIARLGAQYEGILRRYQRRSDDTVRDTVLFSMLADEWPAARAGLTTR
                     LDDLLSH"
BASE COUNT          320 a          641 c          709 g          335 t
ORIGIN      
        1 gatgttccgg tccgtcgcgc agcaggatca ccgtcgcaga cgccttcgcc ggcaccggag
       61 atccacccga ctcgtgatac gcccgtgccc gggcgaccag ttcggggctt ggcagcagcg
      121 gctgctgatc ggcgtccggc ataagggtga gcgtaagtgc gagactgccg ccatgtcctt
      181 cactgctgac gagttggcgt acgcgaccga actcgtgggt cgacgggtac caccgacgcc
      241 gcaatatgcc tggccactgc tgggcgcgca ggtcggcgcg gacgtgtggg tcaagcacga
      301 gaaccacact ccgaccggtg cgtttaaggt ccgcggtggt ctcgtgtacg ccgaccggct
      361 gcgggcgcgg cgaccacagg tgtcgggcat cgtgtcggca acgcgcggca atcacgggca
      421 gagtctcgcg ttcgcgggtc gcgccaatgg cctcgcggtg accatcgtcg tcccgcatgg
      481 caacagtccg gacaagaacg ccgcgatgcg ggggttcggc gccgaggtac tcgagcacgg
      541 tcacgactat caagccgcgc gcgagtactc gatggagctc gcggcggaac gtgagctcga
      601 ccctgtaccg ccctatcacc ctgacctggt cctgggcgtc gcgacgtacg cacgcgaact
      661 cttcgacgca gtcggcgaac tcgacacggt ctacgtcggt gtcggaatgg ccaccggcat
      721 cagcgggctg atcggggtac gcgacctgct ggggctgcga accgagatcg tcggtgtggt
      781 ggccgagatg gcgcccgcca ccgcgctgtc ctttgcggcc ggcaagcaag tgacgaccga
      841 gtcagcgcac acgttcatcg acggcgtggc gtgccgggtg ccggatgcga gcgcgatcga
      901 tgtcattaac gccggcgccg cacggatcct gcaggtcagt gaagacagct gcgcggacgc
      961 cgttcggttg ctgttgtcga ccacccacaa catcgcggaa cccgcaggtg caatcgcact
     1021 ggccggtcta ctgtccgagc gtacgtcggc tcgcggacga cgcgtcgcgg tcgttcagag
     1081 cggcggcaac atcgacagct ctatgctcgc ggagatcctg gccggtcgga cacccgttgt
     1141 ttagtggctg agcaggtcgt cgaggcgggt ggttagtccg gctcgcgcag cgggccattc
     1201 gtcggcaagc atcgagaaca gcacggtgtc gcgaaccgtg tcgtcggacc gtcgctggta
     1261 gcgccgcaag atgccttcgt actgggctcc gagacgggcg atcgcttgtt gtgatcgcgc
     1321 gttgcggacg tcggtcttca gctgcacgcg cccggcgttg agtgtctcga aggcgtagcc
     1381 gagcagcagc agcttcgtct cggggttgac gacgccggcc cagaccgccg gggtgtacgc
     1441 cgtgaagccg atctccagtc gcgcatcgcg cggagaaatc tcgaggtagg acgacgtgcc
     1501 cacgaccgcg ccggcaggga gtccagcctg gtctgtggtc agccgcacga cccatggcca
     1561 ccgaccggcg ctcgctgcct gctcaagagc ctcggtgaaa cccgccacat cgatcggtcg
     1621 gccggcgacg tgctgccaga cggcgccgtg gtcgagtgcg gcgaacaact caccggcatc
     1681 gcgatgcggg tcggccagcg tcagttcgat gcatcgcccg cgcagcacga tgtcttttgg
     1741 tggcggccat tgcaggtgcg gccacgcgac gtcttctggt cgcgcatcga cgcgggggac
     1801 gaaccggggg gttgtcatag tgcgcacatt ctcgctggtg cgcacgcact caggcgaccc
     1861 ggttacgcgg gacgtcagcc accgagccga acccgggctc aggagtgggc cggtggtgct
     1921 tgcgtcgtgc agctcagggt cgtattggtg gggaccaggc gttcaacgga tcccggcagt
     1981 ccatgggtga tggccagttg cgccg
//
LOCUS       DATCBJ010000160         3759 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_500687, whole genome shotgun sequence.
ACCESSION   DATCBJ010000160 DATCBJ010000000
VERSION     DATCBJ010000160.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 3759)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3759)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3759
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_500687"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2099)
                     /locus_tag="VII50_03275"
     CDS             complement(<1..2099)
                     /locus_tag="VII50_03275"
                     /inference="COORDINATES: protein motif:HMM:NF025011.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-associated domain-containing protein"
                     /protein_id="HEY5049895.1"
                     /translation="MSTAQVDLVNWLRGQPDDDLAQLLVARPDLLHPVPSDIEVLAAR
                     AAGRASVEIALDRFDVGALQVVEAMVLSPDPTDLRALAALLDVAPAKLAPALDRLRRA
                     VVVWGRDDQLRLPPVVRDLMPAPAALGPPARALLEHVPRSQLAGLLGDLGLASVGDPT
                     LGIATSVAAVDALADMLENPAQLNALLGRAPDAAREVVELLAAGPPTGRIPAARRPVD
                     AASADSPVRWLLAHALLIAVDDFTVVLPREVAMQVRGGHAFGAVELDPPPGQSTKHKQ
                     AQVDASAAGQAFTAVRLVEALLERWGVEPPGVLRAGGLGVRDLRRTARELELDERAAA
                     LLIEVAHAAGLIAPDAEMADSWLPTTGYDLWLAKSVADRWVILVSAWLTTTRVPGLVG
                     RREPSFSAVSDEVDRPQPAANALGGDLDRALAPIVRREVLEILLRATAGEAPTASSIN
                     ESLRWSWPRRGGRLRDDLAGWTIEEAELLGLTGRGALSSFGRRLCATADSETDAEMVT
                     AGADVAAATSTLLDELLPRPLDHVLLQADLTAVVPGPLESELARELAMTADIESTGGA
                     TVFRFSDTSIRRALDAGRSAADLHALLAARSRTPVPQPLSYLIDDVARRHGRIRIGLA
                     GSYVRCDDDGLLNEVLADRRVESLRLRRLAPSVLVSPLTPERVAERLREVGYAPAAES
                     PDGALLLRRPDAGRAPSR"
     gene            complement(2172..>3759)
                     /locus_tag="VII50_03280"
     CDS             complement(2172..>3759)
                     /locus_tag="VII50_03280"
                     /inference="COORDINATES: protein motif:HMM:NF023260.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3352 domain-containing protein"
                     /protein_id="HEY5049896.1"
                     /translation="LLPGNSGGDMLASGPGDMLASGSVERPRRNLAAILVSLLAVLAV
                     IGGGVAYVGYHKLASSGAQPDQWAPASSIAYLKIDLDPAASEKIAALRFEQKFPDAPH
                     VTSADQLKDALLSEAFNRPSSEIDYATDIKPWLGDRVAIAVYPDSAGAPQPVGILQVK
                     DATKAQAGLAKLIHTPSVSGSGTPGFDIEGDYAIVGPSQAAVDAAVAAARTSSITASN
                     QYAGDVATLGGDRIVTAWADLGALAKYASKSGALTGGLGSSALGDLSGLGGGSGLGSL
                     GSLAQLGGAQGLTTSALKGRLVLGLELQAGYASISGRLIGADVSGVQNGDAGTLLGQL
                     PAGSVAGVAASGIGALISKELTTLESGPLAALGVKGEMDALGARLGISLPGDAINLLG
                     NDVAVGLDSVPAAHGKGKFTAIAEPTDVTQGLKTAQALAGLMTASGTPATATASGSKV
                     SLTNDGAASGALGNDSGFKAAMSGMPDQVVAAAYVNLAGIWPTVGSKVPSDIRHLTGI
                     GTYEALAGSDITFTVRVTVN"
BASE COUNT          556 a         1244 c         1394 g          565 t
ORIGIN      
        1 cgggacgggg ctcgaccggc atctggacgg cggagcagca acgcgccgtc cggactttcc
       61 gccgcagggg cgtagccgac ctcgcgcagc cgttccgcga cccgctccgg cgtcaacggg
      121 ctcaccagaa ccgacggcgc gaggcggcgc agtcgcaggc tctcgacccg acgatcggcc
      181 agcacttcgt tcagcagtcc gtcgtcgtcg cagcgaacgt aggaaccagc gagaccgatc
      241 cggatccgcc cgtgccggcg ggcgacgtca tcgatgagat agctgagcgg ttggggaacc
      301 ggcgtacgtg accgggcggc gagcagcgcg tgcaggtcgg ctgcgctgcg cccggcatcg
      361 agcgcgcgcc ggatcgaggt atcgctgaac cgaaaaacgg tggcgccgcc ggtcgattcg
      421 atgtcggcgg tcatcgcgag ttcgcgggct agctcgcttt ccagtggtcc gggtacgacc
      481 gcggtgaggt cggcctgcag cagcacgtgg tcgagcgggc gcggaagcag ttcgtcgagc
      541 aacgtcgacg tggctgcagc gacatcggca cccgcggtca ccatctccgc gtccgtctcg
      601 gagtcggcag tggcgcacag acgccgaccg aacgaggaca gggcgccgcg accggtcagg
      661 ccgagcaact cggcttcttc gatcgtccag ccggccaggt cgtcgcgcag ccgcccgccg
      721 cgtcgcggcc atgaccagcg cagcgactcg ttgatcgagc tcgcggtcgg agcttcgccc
      781 gcggtagcgc ggagcaggat ctcgaggacc tcgcggcgaa cgatcggggc cagcgcccga
      841 tcgaggtcgc cgccgagtgc gttcgcggcc ggttgcggac gatcgacctc gtcgctcacg
      901 gccgagaagg acggttcgcg acggccgacc agtcccggca cccgggtggt cgtcagccac
      961 gcgctgacga ggatcaccca gcggtcggcg accgacttcg ccagccacag gtcgtagccg
     1021 gtcgtcggca gccagctgtc ggccatctcg gcgtcggggg cgatcaggcc ggcggcgtgc
     1081 gcaacctcga tgagcagcgc ggctgcccgt tcgtcgagtt cgagttcgcg tgcggtgcgt
     1141 cgcagatcgc ggacgccgag gccgccggcc cgcaagaccc cgggcggctc gacaccccaa
     1201 cgctcgagca gcgcttcgac cagccggacg gcggtgaagg cctggccggc ggcgcttgca
     1261 tcgacctgcg cctgcttgtg tttggtgctc tgacctggcg gtggatcgag ctcgaccgca
     1321 ccgaacgcgt gcccgccgcg cacttgcatc gcgacctcgc gcggaagcac gacggtgaag
     1381 tcgtcgacgg cgatcaggag ggcgtgcgcg agcagccagc gcaccggcga atcggcgctg
     1441 gctgcgtcga ccggacgccg agccgccggg atccgaccgg tcggtggccc ggcggcgagc
     1501 agttcgacga cctcgcgagc ggcgtccggc gcgcggccga gcaacgcgtt cagttgggcc
     1561 gggttttcga gcatgtcggc gagcgcgtcg acggcagcga cggaggtagc gatgccgagg
     1621 gtggggtcgc cgacactcgc gaggcccagg tcgcccagca gcccggcgag ctggctgcgc
     1681 ggaacatgtt cgagcaaggc gcgcgcgggt ggtccgagcg ccgcgggtgc cggcatcaga
     1741 tcgcggacga cggggggcag ccgcagttga tcatcgcgac cccagaccac gacggcccgc
     1801 cgcaaccggt cgagcgcagg cgcgagcttg gccggtgcga cgtcaagcaa agcggctagg
     1861 gccctgaggt cggtcgggtc gggagacaac accatggcct cgacgacctg cagggcgccc
     1921 acgtcgaatc ggtcgagcgc gatctcgacg gatgcacgcc ctgctgcccg tgccgcgagc
     1981 acctcgatgt cggacggaac cgggtgcagc aggtcgggcc gggccacgag cagttgcgcc
     2041 agatcgtcgt caggctgccc acgcaaccag ttgacgagat cgacctgggc tgtgctcatc
     2101 cggtcaacgg tagtcggttc ttctctactg ccgtgctgtc gcgagcatgg gtggggaaat
     2161 cccgacctga actagttgac ggtcacccgg acggtgaagg tgatgtcgct gccggcgagc
     2221 gcttcgtagg tgccgatgcc ggtcaagtgt cggatgtcgc tgggcacctt gctgcccacc
     2281 gtcggccaga tcccggcgag gttgacgtag gcggcagcga cgacctggtc gggcatgccc
     2341 gacatcgccg ctttgaaccc tgagtcgttg cccagtgcgc cgctcgctgc cccgtcgttg
     2401 gtgaggctga ccttggaacc ggacgccgtg gccgtggcgg gagtgccgct ggcggtcatg
     2461 agcccggcca gtgcttgcgc ggtcttgagg ccctgagtga cgtcggtcgg ttcggcgatg
     2521 gcggtgaact tccccttgcc gtgggcggcc ggaacactgt cgaggccgac cgcgacatcg
     2581 ttgcccaaca ggttgatcgc gtcgccgggc aggctgatgc cgagcctggc gccgagggcg
     2641 tccatctcac ccttcacgcc cagcgctgcg agcgggccgg attcgagggt ggtcagttct
     2701 ttgctgatga gcgctccgat acctgaggct gcaacacccg cgaccgaccc ggcgggcagc
     2761 tgacccagca aagtgccggc gtcgccgttc tggactccgc tgacgtcggc gccgatcagg
     2821 cggccgctga tggaggcata gccggcttgg agctcgaggc cgaggacgag gcggcctttg
     2881 agtgcgcttg tggtcaggcc ctgggcgccg cccagctgcg caagactccc gaggctgcca
     2941 aggccgctgc cgccaccaag cccgctcaga tcgccgaggg cgctgctgcc caagccgccg
     3001 gtcagcgcac ccgacttgct ggcgtacttg gccagtgcgc cgaggtcggc ccacgcggtc
     3061 acgatccggt cgccgcccag tgtcgcgacg tcgccggcgt actgattgct tgcggtgatg
     3121 ctcgacgtgc gggcggcggc gacagctgcg tcgaccgcgg cttgcgacgg gccgacgatc
     3181 gcgtagtcac cctcgatgtc gaatccgggc gtgcccgagc cggaaacgct cggtgtgtgg
     3241 atcagtttcg cgaggccggc ttgcgccttc gttgcgtcct tcacttggag gatgccaacc
     3301 ggctgtggcg cgccggcgga gtcagggtag accgcgatcg cgacccgatc gccaagccag
     3361 ggcttgatgt cggtcgcgta gtcaatctcg cttgagggcc ggttgaaggc ttcgctgagc
     3421 agggcgtcct tgagttggtc ggcgctggtg acgtgtggag cgtccgggaa cttctgttcg
     3481 aatcgcaggg cggcgatctt ctccgacgcg gccggatcga gatcgatctt gagataggca
     3541 atcgaggagg cgggtgccca ctggtcgggt tgggcgcctg agctcgcgag cttgtggtag
     3601 ccgacgtaag cgacgccgcc gccgatgacg gcgagtaccg cgagcaggct gacgaggatc
     3661 gccgcgaggt tgcggcgcgg ccgctcgacc gagccggagg cgagcatgtc gccggggccg
     3721 gaggcgagca tgtcgccgcc cgagttgcct ggcagcagc
//
LOCUS       DATCBJ010000161         6528 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_205680, whole genome shotgun sequence.
ACCESSION   DATCBJ010000161 DATCBJ010000000
VERSION     DATCBJ010000161.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 6528)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6528)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6528
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_205680"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..236)
                     /locus_tag="VII50_03285"
     CDS             complement(<1..236)
                     /locus_tag="VII50_03285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5049897.1"
                     /translation="MPSPRVVSGIIAGRARGAVVTLNISDVGVGFDARRALVYLPPQY
                     FSNPDERFPVVYLLHGSPGMPVDWLRGGGAATAGL"
     gene            274..396
                     /locus_tag="VII50_03290"
     CDS             274..396
                     /locus_tag="VII50_03290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5049898.1"
                     /translation="MSEATTVGALLSGTGLADTLRGDATAGVEGSVAAGWMART"
     gene            complement(432..653)
                     /locus_tag="VII50_03295"
     CDS             complement(432..653)
                     /locus_tag="VII50_03295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5049899.1"
                     /translation="MGALSVIDAGFLLVLAGGSALLWIASWLARRRRWRRVLLAGCAA
                     LAAITFAADAVNAYFQYLPRVSDVLGQRD"
     gene            complement(1181..1570)
                     /locus_tag="VII50_03300"
     CDS             complement(1181..1570)
                     /locus_tag="VII50_03300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009076541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY5049900.1"
                     /translation="MFENTAAYSGFSVDDAAKAKEFYGTTLGLRVTEDHGMLALHISG
                     NNRATLIYPKADHTPATFTILNFPVDDIDAAVDELAKRGIEFERYGGDLKADSKGILR
                     GIAANMSPDIAWFKDPAGNILSVLHEA"
     gene            2568..2969
                     /locus_tag="VII50_03305"
     CDS             2568..2969
                     /locus_tag="VII50_03305"
                     /inference="COORDINATES: protein motif:HMM:NF025063.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integrase core domain-containing protein"
                     /protein_id="HEY5049901.1"
                     /translation="MGAQKLRCAAATHGSGGIGRAVHVLDPLVGTRGSTAAFDGVFAA
                     ISARGDSRAGADAEANVFAERFVGTVRREWLDHLLVINEAHLRAVLADVARQCNQHRP
                     HQGRRHQAPTNVPGASWTRPQPSKDGRSSAD"
     gene            3087..3302
                     /locus_tag="VII50_03310"
     CDS             3087..3302
                     /locus_tag="VII50_03310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5049902.1"
                     /translation="MALLAGDAARQRHTARGIMALTQGISGSSWSDSGLRSGSTRDSC
                     SPKDIAAMKNVSGCHEALAGVVEHSPI"
     gene            3586..>6528
                     /locus_tag="VII50_03315"
     CDS             3586..>6528
                     /locus_tag="VII50_03315"
                     /inference="COORDINATES: protein motif:HMM:NF016047.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding repeat-containing protein"
                     /protein_id="HEY5049903.1"
                     /translation="MHARSFRPAIAHRFAGSARRVGVAATVVVVGLGGVIATASPSIA
                     TPASPTVTVLNNVGTPASNVAVGATLAVTAAAPITVAGATSQTITQTWNAAQSLLTPS
                     SIVTPEGWTKTYTTDGTTWSSTLPTDPTTVSGVQSTGAVFSDGTSSGLQVSTATGTGT
                     LKTAPVFAPSSGGDGWDAFTSGLYVMNAWHHNGGSYNLDCHLIATGVSCGSVYSVSGY
                     ETSTSSSGTVDAGKVYSIVGQIASNSVGVLCTNVSALPFAPCGYVALEVGTANYTIIG
                     SAQQSGTKIYAPLNDGNVVCFDISTGAACVGQPYALASYTGSAGTQITAFSTAVGPYI
                     FITASKIWCLTAADGSACAGVWPVGSYSTNLLTGAVPMRDTAGTLTGVCMIRPAGNCF
                     TLTGATTTMPAGLTSLLASSEPDAGISIGGYGQFGFGATKQYWFAGGWGPDTPACYDW
                     TTDAACAGFQTTTPIGNVRYTIRVDATNPNCLWSNGDDGSIAPFDANTGQPGCQGSDP
                     VVDIPYTAVVPRLSCTEAGRIRNWQNITFTPPGAIAMTALRVTVLDSSGVAIAGYTSL
                     TPNSSGVVDLSALAVATSGTQPTFEVTAIGASSPNASAISAAVTYTSDAPQLCFTLTT
                     IHNCPTLAPGVSAGATVAVSDVALGGSTVATASGVPTTTPLSAAITRANMAGCVGTVS
                     GTATQTFTSGAPDAPLANGTVYLLDSFGTAVASTVTAADGTYSFANANPAAYTAQMSL
                     VTHALTVTAGGSAVQNFSVPYVAPTKPATAAFTQLGGANRDATAAMVATTMYPLAGSA
                     SVVVLARDDLYADGLTGSPLANAFGGPLLLSPTASLSSATQAAIAHVLMPGGLVICLG
                     GTSAISPSVAATLQQLGYQVERIGGVDRYATATLIADKIGTKHAVAHVYVATGLNFAD
                     ALSAADAAGLNNGVVLLTADTIMPGSTNTWLAAHTSAPATAIGGQAAAADPTATALVG
                     SNRYATAAL"
BASE COUNT         1130 a         2209 c         2091 g         1098 t
ORIGIN      
        1 agaccggcgg ttgctgcgcc accaccgcgt agccagtcca ccggcatccc cggggagccg
       61 tgcagcaagt agacgacagg gaatcgttcg tccggattgc tgaagtattg cggcggcaag
      121 tacaccaggg ctcgtcgcgc atcgaaccca actccaacgt cgctgatatt caaggtgacg
      181 accgcgccgc gagctcggcc cgcaatgatg cccgacacga cgcgcggcga cggcacggcg
      241 gcttgggggc tggcgacgcc tccaggggcc gccgtgagcg aggcaacaac ggtcggcgcg
      301 ctgctttcgg gcaccgggct tgccgacacg cttcgagggg acgccaccgc gggcgtcgaa
      361 gggtctgtcg ccgctggttg gatggcgcga acgtaggcta tttcgtgaag tgccgcttgc
      421 gggacaaccg gtcagtcgcg ttgcccgagc acgtcgctga cccgcggcag gtactggaag
      481 taggcattga ccgcatcggc ggcgaaggtg atcgcggcca gcgccgcgca ccccgcgagc
      541 agtacgcgtc gccaccgacg gcggcgagcc agccaactcg caatccacag cagggccgat
      601 ccaccggcca gcaccagcaa gaagccggcg tcaatgaccg ataacgctcc catgccgcca
      661 gtgtgccagc ccccgcggca gcctgacctg gttcagtcgg cccagccttg aagtgatccc
      721 ggtcaacgca taatccaggt catcgcgccc aacaaacccc tgccaccaac agagctggac
      781 ggcgcctcag cacagctcca tcccgcccag tgcacctggt ccgggcgagc cccgctcgca
      841 tcctcgatga aatccgaccc gggggagccg cttggctgag ctgcccggcc ggcaggtcgc
      901 ggggtgtgtt gcgatgtcgg gatctgttga tcaacgcgtc atccttgcgg ttggagatag
      961 cgctgacgcg ccgacgcgca acgaccagag ggggaccagg gggtcaacat gttgatgtgc
     1021 ctgcgagggg acgcgcctgc gcgcgtgcga gcccacaccg cgcttcgggg tgagcggatc
     1081 gacgtggcgc agttcgctat cgtgcccgac ctcgacatgg ttggcgctcg cggtgcgggg
     1141 atcgcagcgc gcacgatcgt cctcggagat tgaccgccga tcaggcttcg tgcagcacgg
     1201 acaggatgtt tcctgccgga tccttgaacc aggcaatatc gggactcatg ttggccgcaa
     1261 tgcctcgcag gatgcccttg ctgtcggcct tgagatcgcc gccgtagcgt tcgaactcaa
     1321 tgccccgctt ggcgagttcg tcgacggcgg cgtcgatatc gtcgacgggg aagttgagga
     1381 tcgtgaatgt ggcgggcgtg tggtcggcct tcgggtagat cagcgtcgcc cggttgttgc
     1441 ctgagatgtg cagggcgagc attccgtgat cttcggtgac ccgaaggccc agggtcgtcc
     1501 cgtagaactc cttcgccttg gcggcgtcat cgaccgagaa tccgctgtag gcggcagtgt
     1561 tttcgaacat gacgctccat cccgttgcgt ggccagccga ggtcaggtat ctccgctcgc
     1621 agcctaggac ggcgccggcc ggtcgaactc atcggtaggt gacgaactcc ccgacgtggg
     1681 acgggtcggg ctggggtgcg gtcgaccggt gcgccgtgac ctttctgttc gacaccgcgg
     1741 tagaccgcgt ttcccgatga acgcgtcatc ctcgcgattg gagatggcgc tgacgccaac
     1801 gtgcgacgac cagcggggga ccgggtgggg accaacatgt tgatgtgcct gctagggaac
     1861 gcgtctgtcc gcttggacgt ctggggtggc aaccatgggt gcaccaaaag cagcgacgta
     1921 ggcccctgac ctgcacaaac agtgtgtccg ggaggggact tgaaccatct gcaccgccgg
     1981 tgttggttga gcgtgaatcg ggccaaaatc gcggctttgc ctgcggtgta agggaaaacg
     2041 agcgcccgga tgctgaccgt tgcgccccgt tgcaacggat tggtgcaacc accgggggca
     2101 cggatgatct tgatccggtt ccgaggaaca acctcttctt cgggtaggcc gagcggttcg
     2161 atgacctacg gcttgacgag caagtggacg caagccttac gcgcgccgtt ggcctccgcg
     2221 atccgtcaga tgcgcccgcg acacgctgca cggtcacgcc ggacagccca aacaacaacg
     2281 tcgtagcatt actcaagccc gtggcctctt gagcgcgcga aaacccttgc tgtgcaaggg
     2341 ttctcgatgg agtgggtgac cgggagcgca ccggcctcgg cgacttggaa ggctagcggg
     2401 acacgttccc gtttgatcgt gaaaatggcc gatgacctgc caatatatgt ttttgatctt
     2461 gaccgctgtg gtccgtgtta ggcctcaatt taccggccgt acggccccgc tcgacgggtt
     2521 tccgtccacc tcattgaaag ggggtctgtg gctacaccct cgacggggtg ggagcccaga
     2581 aactgcgctg cgccgccgcg actcatggat ctgggggaat cgggcgggcg gttcacgttc
     2641 ttgatccgtt ggtcgggacg cgaggttcta ccgcagcctt cgacggcgtc ttcgcggcga
     2701 tcagcgcccg gggtgattcg cgcgccggtg cggacgccga ggcgaacgtg ttcgccgaac
     2761 gattcgtcgg caccgtccgc cgggaatggt tggatcacct gctggtcatc aacgaggccc
     2821 acctacgagc agttctggct gacgtggccc ggcagtgcaa ccaacaccgt ccccatcagg
     2881 gacgacggca tcaggcgccg accaacgtgc cgggcgcgtc gtggacccga ccccagccat
     2941 ccaaagacgg taggtcctcg gcggactgat cagccagtac cggcgagccg catgaccgcg
     3001 atcacgcgcg cgccgcggag caggacgcgt tttggcacgg gacagggcgg cttgaggacc
     3061 tgaccggccc gcagggaact ctgcagatgg cattgctcgc tggcgatgcc gcccgccagc
     3121 gccacacagc gcgcgggatc atggcgctca cccaaggcat ttctgggtca agctggagcg
     3181 actccggtct ccgcagcggc tcgacgcgag acagctgcag ccccaaggac atcgccgcga
     3241 tgaagaacgt gtccggctgc cacgaagccc tggccggggt cgtcgagcac tcacctatct
     3301 gatgactcgg gcgatcccaa taccggttcg acaggccggc tagtagcatc cgcggaccaa
     3361 aactcgcgac cgctgatctg gccgttcgca atcggcttca gcgccaaagt ggtcgccggt
     3421 gtagcgctcc acatccgcat gggtcacgcg cgcgctcacc gccgggcgat cgccgtctcg
     3481 ggttcccgag cacctagccg gccggaatcc cttcgcaatc gctttcgccg gccgaagggg
     3541 caagtagtgc tcatgccacg tgggtgcatc caaccaggag ttcccatgca cgcacgctcg
     3601 tttcgtcccg caatcgccca tcgcttcgca gggtccgccc gcagggtcgg cgtcgcggcc
     3661 acggtcgtcg ttgtcgggct cggtggcgtg atcgccactg cctcgccgtc catcgccacg
     3721 cccgcctcgc caacggtcac cgtgctgaac aacgtcggca ccccggcgtc caacgtggcg
     3781 gtcggcgcga cgctcgccgt cacggcggcg gcgccgatca cggtcgctgg cgcgacctcc
     3841 cagacgatca cccagacctg gaacgccgcg cagtcattgc tcaccccgtc gagcatcgtg
     3901 actcccgaag gctggacgaa gacctacacg accgacggga cgacgtggag cagcacactg
     3961 ccgactgacc ccacaaccgt cagcggagtg cagtcgaccg gcgccgtctt cagtgacggg
     4021 acgtcgagtg gcctgcaggt ctcgaccgcg actggcaccg gaacgctgaa gactgcaccg
     4081 gtgttcgcgc cttcatcagg tggcgacggc tgggacgcct tcacctcggg cctctatgtg
     4141 atgaacgcct ggcatcacaa cgggggttcc tacaacctcg actgccatct catcgccacc
     4201 ggcgtctcgt gcggaagtgt ttactcggtc agcggctatg aaacgtcgac gtccagcagc
     4261 ggaaccgtcg acgccggaaa ggtctattcg atcgtgggcc aaatcgcgtc gaactcggtc
     4321 ggcgtgctgt gcaccaacgt gtcggcactg ccgttcgctc cgtgcggcta tgtcgcactg
     4381 gaagtcggta cggcgaacta caccatcatc ggatcggcgc agcagtccgg caccaagatc
     4441 tacgcaccgc tcaacgacgg caatgtcgtg tgcttcgaca tctcgacagg tgcggcctgc
     4501 gtcggccagc cgtatgcgct ggcgtcctac accggtagcg caggcactca aattacggcc
     4561 ttcagtaccg cagtcgggcc ttacatcttc atcaccgcca gcaagatctg gtgcctgacc
     4621 gccgccgacg gctccgcgtg cgcgggagtc tggccggtcg gctcgtacag cacgaacctt
     4681 cttactggcg ccgtcccgat gcgcgacact gcaggcacgc tgaccggcgt gtgcatgatt
     4741 aggcccgccg gcaactgctt cacgctcacc ggcgcgacca ccacgatgcc tgcgggactg
     4801 acctcacttc tcgcgagcag cgaaccggac gcggggatca gcatcggcgg ctacggacag
     4861 ttcgggttcg gcgccacgaa gcagtattgg ttcgccggag ggtggggtcc cgacaccccg
     4921 gcttgctacg actggacgac cgacgccgcc tgcgccggct tccagaccac gacgccgatc
     4981 ggcaacgttc gctacacgat cagggtcgac gccaccaacc ccaactgcct gtggtcgaac
     5041 ggcgacgacg gctccattgc gcccttcgat gcgaacaccg gtcagcccgg ctgccagggg
     5101 tcagatccgg tcgtcgatat cccttacacc gctgtggttc cgcggctgag ctgcaccgaa
     5161 gcaggccgga tccgcaactg gcagaacatc acgttcaccc cgccaggcgc catcgcgatg
     5221 accgcgctac gggtcacggt tctggattcc tccggcgtcg cgatcgcggg ctacacaagc
     5281 ctcaccccga acagcagcgg agtcgtcgat ctctcggcgt tggccgtcgc cacatcgggc
     5341 acgcagccca ccttcgaggt caccgccatc ggcgcatcgt cgccgaacgc cagtgccatc
     5401 agcgcggcgg tgacctatac ctccgacgca ccgcagctat gcttcaccct caccacgatt
     5461 cacaactgcc cgacactggc ccccggcgtt tcggcaggtg cgacagtcgc cgtatctgac
     5521 gtcgctctgg gcggatcaac cgtggcgacc gcctcaggtg tgccgaccac gacgccgttg
     5581 tcggcagcaa tcacccgagc gaacatggcg ggctgcgtcg gcacggtcag cggcaccgca
     5641 acacagacgt tcactagcgg cgcaccagac gcgccgctcg ccaacggcac ggtgtatctg
     5701 ctcgattcgt tcggtactgc cgtcgcttcg acggtgaccg ccgccgacgg tacgtactcg
     5761 ttcgccaacg ccaatccggc ggcctacacc gcgcagatgt cgctggtgac ccacgcactg
     5821 accgtcactg caggcggctc ggccgtgcag aacttctcgg tgccgtacgt ggctccaaca
     5881 aagcctgcca ctgcggcgtt cactcagctc ggcggcgcca atcgtgacgc cactgcggcg
     5941 atggtggcga ccacgatgta tccgctcgca ggttcggcaa gtgtcgtggt cttggctcgt
     6001 gacgatctgt acgcggacgg gctgaccggt tctccgttag ccaatgcgtt cggcggcccg
     6061 ttgttgctga gcccgaccgc gtcgttgtcg agcgccaccc aagcggccat cgctcacgtg
     6121 ctcatgcctg gcggcttggt gatctgtctc ggcggaacaa gcgcgatcag tccttcggtc
     6181 gcagcgaccc tgcaacagct cggctatcag gtcgagcgca tcggcggcgt cgaccgttac
     6241 gcgaccgcga cgctcatcgc cgacaagatc ggtaccaagc acgccgtcgc gcacgtctat
     6301 gtcgccaccg ggctcaactt cgctgacgcg ctgtcagcag ccgacgcagc ggggctcaac
     6361 aacggcgtcg ttctactcac cgccgacacc atcatgcccg gatcgacgaa cacctggctc
     6421 gctgcgcaca ccagcgcacc ggccacggcg atcggcggtc aggcagcagc ggctgatccc
     6481 accgcaacgg cgttggtcgg cagcaaccgg tatgcgaccg cggcgctc
//
LOCUS       DATCBJ010000162         4360 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_20895, whole genome shotgun sequence.
ACCESSION   DATCBJ010000162 DATCBJ010000000
VERSION     DATCBJ010000162.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 4360)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4360)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4360
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_20895"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..302
                     /locus_tag="VII50_03320"
     CDS             <1..302
                     /locus_tag="VII50_03320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5049904.1"
                     /translation="LYVTVAGLIGLALGALLRNTAAAISAFVEAFFVVPQLTMLLPSS
                     ISTNLNPYLPSNAGAAVFGGGPAHALSPWIGFIVFCGYAIALITAAAYRLQRGDA"
     gene            329..970
                     /locus_tag="VII50_03325"
     CDS             329..970
                     /locus_tag="VII50_03325"
                     /inference="COORDINATES: protein motif:HMM:NF025687.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4386 family protein"
                     /protein_id="HEY5049905.1"
                     /translation="MLALALPITAALYIGAEGLDPRGTDKLVTTTAVAYKVLPIAAKH
                     STQLFVSGSLSELALGGVAVSYAVIAMLVRKRGSTVATVAAMIGAIGAFCGAVVNVFV
                     GINLAAAATAHVAQDAAARILVATFNSGAGQAFTDVYAFEEFVSPVLMGIALWRSGTV
                     PRWLAVLFAAGFELAQQTASVGIVKVVLLTAPFAVAMVALSVRIWQAKVATES"
     gene            1017..1988
                     /locus_tag="VII50_03330"
     CDS             1017..1988
                     /locus_tag="VII50_03330"
                     /inference="COORDINATES: protein motif:HMM:TIGR01188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY5049906.1"
                     /translation="MSVIGLTKSYGELEAVRGIDFTIGAGETFGFLGPNGAGKSTTIS
                     MLCTLITPTSGTATVAGYDVVTQRSDVRRNIGLVFQDTTLDGYLTAERNLRLHAELYG
                     IPRGMVDDRLRQVMDMVGLWDRRSSKVSTFSGGMKRRLEIARGLMHSPRVLFLDEPTV
                     GLDPQTRSSIWSYIRELKEAEDITIFLTTHYMDEAEYCDRIAIMDHGQIIALDTPESL
                     KASIGKDRVQIHTDDDTAAIAALESHFGLSAKVADGAVTFGVEHGEEFVPRLFAGFPL
                     AIRSVNVSRPTLDDVFMSYTGSTIRDAEATTGQRGRDAMQMFRAGRR"
     gene            1985..2854
                     /locus_tag="VII50_03335"
     CDS             1985..2854
                     /locus_tag="VII50_03335"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY5049907.1"
                     /translation="MTLTTSGLPVASVRIPVRTAASELRAIKIVWQRELIRFFADRLR
                     IITALVQPFLFLFVLGSGLSHLTQGGTGGLNLKTFLYPGILAMAVLFTAMFSAASIVW
                     DREFGFLREMLVAPVRRSSIVIGKCLGGATVAGFQGIIVIAIAGLVGVPYAPTLMLAV
                     FGIQLLLAFTITTFGVMMAARVRQMQSFMALTQMLLLPLYFLSGAMFPASNLPVWLTI
                     ANRIDPLTYAVDPIRRVVFDHLHLSAAVRAKLDPGVTWDGWRVPTLLEVAVVAVLGLV
                     MLCIAIAEFNKSE"
     gene            2932..3852
                     /locus_tag="VII50_03340"
     CDS             2932..3852
                     /locus_tag="VII50_03340"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HEY5049908.1"
                     /translation="MHQISGVRDPSSSFRLSGVLDEAGDRRQGNRAVGWGGLGLAATG
                     LIELALALLSGSVALLGDALHNLSDVSTSLLVFIGFRASRKTPTDRYPYGFERAEDLS
                     GVGIAIVIWLSAVLAGAESVDKLVRHGSTHYVGWGIAGAAVGILGNQAVARFKLRIGK
                     QINSATLIADAKHSWLDALSSTGALLGLVGVLCGLKWADGVAGIVVTGFICHVGWEVT
                     SDVVHRLLDGVDPQIVLTAESAAAEVPGVVHAHARARWTGRTLRLEVEGWVGSDVSVA
                     EADRIGQAVADELSTQLPDMRSFSWTARGI"
     gene            complement(3863..4264)
                     /locus_tag="VII50_03345"
     CDS             complement(3863..4264)
                     /locus_tag="VII50_03345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5049909.1"
                     /translation="MTRFVIDPPTLVQLAQDDRRIDPAHQLVAPNSIRSQALDLLLQR
                     VRDGELAEKAALDLHERMTEVKIRLLGDRVCRRIAWRTAREHNWTTTRDAEYLAIAKL
                     QADALIATDPDLAAKADGLVPLAALNELFLD"
BASE COUNT          688 a         1405 c         1428 g          839 t
ORIGIN      
        1 cgctatacgt gacagtcgcg ggactgatcg ggctcgccct cggcgcgctg ttgcgcaaca
       61 ccgcggccgc catctcggcg ttcgtcgaag cgttcttcgt tgtcccgcag ctgacgatgc
      121 tgctgccgtc gtcgatcagt accaacctca acccctacct gccgtccaac gccggagctg
      181 ccgtcttcgg cggcggcccc gcacacgcgc tgtcgccgtg gatcggcttc atcgtgttct
      241 gcggatacgc gatcgctctc atcaccgctg ccgcctaccg gctccagcgt ggcgacgcat
      301 gaacaacgct gcgcaaacgc gtcggcgcat gttggcgctt gcgcttccga tcaccgccgc
      361 tctctacatc ggtgcggaag gtctcgatcc ccgagggacc gacaagttgg tcacgacaac
      421 ggcggtcgca tacaaggtgc tcccgatcgc cgcgaagcac tcgacgcagc tcttcgtgtc
      481 gggctcgctg agcgaactcg cgctcggggg cgtggcggtc tcctacgcgg tgatcgccat
      541 gctggtgagg aagcgcggtt cgacggtggc gaccgtcgcg gcgatgatcg gagccatcgg
      601 ggcgttctgc ggagctgtcg tcaacgtgtt cgtcggcatc aatctcgctg ctgctgcgac
      661 agcgcacgtc gcccaagatg cggcggctcg gatcctcgtc gcgaccttca actccggcgc
      721 aggtcaggca tttaccgacg tttacgcatt cgaggagttc gtctcgccgg tcctcatggg
      781 gatcgccctg tggcgaagcg ggacggtgcc gcgctggctc gccgttctct tcgccgccgg
      841 cttcgaactc gcccaacaga cggcctcggt gggcatcgtc aaggtcgtgc tgctcacggc
      901 gccgttcgcg gtagccatgg ttgccctgtc ggtccgaatc tggcaggcga aggttgcaac
      961 agagtcgtag cctggaccgg acaacgagat ggggtacacg gatgccggat ccggcagtgt
     1021 cggtcatagg cctgacgaag tcctacggtg aactcgaagc ggttcgcggc attgatttca
     1081 ccatcggcgc cggtgagacg ttcggattcc tgggccctaa cggcgccgga aagtccacga
     1141 cgatcagcat gctctgcacg ctgatcactc cgaccagcgg aaccgcgacg gttgccggct
     1201 acgacgtggt tactcagcgc agcgacgtcc ggcgcaacat cgggctggtc ttccaagaca
     1261 ccaccctgga cgggtatctg accgcagagc gcaacctgcg gctgcacgcc gagctgtatg
     1321 gaattccgcg cggcatggtc gatgaccggc tgcgccaggt gatggatatg gtcggccttt
     1381 gggaccgtcg ctcgagcaag gtctcgacct tctcgggcgg tatgaagcgc cggctcgaga
     1441 tcgcgcgcgg cctcatgcac tcaccgcgcg tgctctttct cgacgagccg acggtcggcc
     1501 tcgacccgca gacgcgcagc tcgatctggt cctacatccg cgaactcaaa gaagcagagg
     1561 acatcacgat cttcctgacc acgcactaca tggacgaggc cgaatactgt gaccgcatcg
     1621 cgatcatgga ccacgggcag atcatcgccc tcgacacgcc tgagtcgctg aaagccagca
     1681 tcggcaagga tcgcgtgcaa atccatacgg acgacgacac tgcggcgatc gccgcgctcg
     1741 agtcacactt cgggctcagc gcgaaggtcg ccgacggcgc cgtgacgttc ggagtcgagc
     1801 acggtgagga attcgtgccg cggctgttcg ccggcttccc gctcgcgatc cggtccgtca
     1861 acgtgtcacg accaacattg gatgatgtgt tcatgtcata caccggctcc acgatcaggg
     1921 acgccgaggc gacgacgggc caacgtggcc gcgatgcgat gcagatgttc cgggcgggac
     1981 ggcgatgaca ctcaccacct cagggcttcc cgtcgccagc gtccgtattc cggtccggac
     2041 ggcggccagc gagctgcggg cgatcaagat cgtctggcag cgggagctga tccgattctt
     2101 cgctgaccga ctgcggatca tcacggcact cgtgcagccg tttctgtttc tgttcgtgct
     2161 tggttccggc ctctcgcacc tgacgcaggg cggcaccggt ggactcaacc tgaagacgtt
     2221 cctctatccc ggcatcctcg cgatggccgt gctgtttacc gcgatgttct cggccgcctc
     2281 gattgtgtgg gaccgggagt tcggcttttt gcgcgaaatg ctcgtggcgc cggtgcgccg
     2341 cagctccatc gtcatcggca agtgcctcgg cggggcaacc gtcgccggct tccagggcat
     2401 catcgtgatc gcgatcgccg gcctggtggg agtgccctac gcgccgacgc tcatgctcgc
     2461 cgtcttcggc attcagctac tcctcgcctt cacgatcaca accttcggcg tcatgatggc
     2521 tgctcgcgtt cggcagatgc agtcgttcat ggcgctgacg cagatgttgc tgctgccgct
     2581 gtacttcctg tcgggcgcga tgttcccggc gtccaacctg ccggtctggc tcaccatcgc
     2641 caaccggatc gacccgctga cctacgccgt cgacccgatc cgccgggtcg tcttcgacca
     2701 cctccacctt tccgcagcgg tacgcgcgaa gctcgatccc ggtgtcacct gggatggctg
     2761 gcgcgtaccg accctgctcg aggtcgccgt cgtcgcagtc ctcgggctgg tcatgctgtg
     2821 catagcgatc gccgagttca acaagagcga ataggcgcgg gctgcgaagc tgagttcagg
     2881 gcgcacgggc gccgcaccga ggttggctgc ggggtctgcg atccttgaaa catgcatcag
     2941 atcagcggag tgcgtgaccc ttcgtcatcg tttcgtctga gcggggttct ggacgaggcg
     3001 ggtgatcgtc ggcaggggaa ccgagccgtg gggtggggcg ggctgggtct cgccgccacg
     3061 ggcctgatcg aactcgcgtt agcgctgctg agcggttcgg tcgccttgct tggtgacgcg
     3121 cttcataacc tgtcggacgt gtccacgagc ctgctggtgt tcatcggctt tcgcgcgtcc
     3181 cgcaagacac ccaccgacag atatccctac ggcttcgagc gagccgagga tctgtcgggt
     3241 gtcggtatcg cgatcgtgat ttggctcagt gctgtgctgg ccggcgcgga gagcgtcgac
     3301 aagcttgtcc gccacggcag cacgcattac gtcgggtggg gtattgctgg tgcagcggtc
     3361 ggcatcctcg ggaaccaagc cgtcgcgcgg ttcaagcttc ggatcggcaa gcaaatcaac
     3421 tcggccactc tcattgcgga cgcgaaacat tcctggctcg acgcgctctc ttcgaccggg
     3481 gcgctcctcg ggctggtggg ggtgctgtgt ggtttgaagt gggccgacgg cgttgccggc
     3541 atcgtggtca ccggcttcat ctgccacgtc ggatgggaag tgacctccga cgtggtgcac
     3601 cgactgctgg acggtgtcga cccgcagatc gtcctgacgg cagaaagcgc tgccgctgaa
     3661 gtaccgggcg tggtccatgc ccacgcccgc gcccggtgga ctggccgcac cctacggctg
     3721 gaagtcgaag gctgggtcgg ctcggacgta tcggtggccg aagccgatcg gatcggccag
     3781 gcggtggccg acgaactcag tacccagttg cccgacatgc gcagcttctc gtggacagcc
     3841 agaggtatct gagccccagc ggtcagtcca ggaagagctc gttcagtgcg gcgagcggca
     3901 ccaacccgtc ggccttggcc gccagatccg gatctgttgc tatcagtgcg tcggcctgaa
     3961 gcttcgcgat cgccaggtat tcggcgtcgc gggtcgtcgt ccagttgtgc tcgcgtgctg
     4021 tgcgccaggc gatacgccgg cagacccggt ctccgaggag tcggatcttc acctccgtca
     4081 tccgctcgtg gagatcgaga gctgcctttt ccgcaagttc gccgtcgcgc accctttgca
     4141 ggagcaggtc gagcgcctgc gagcgaatcg agttcggtgc gaccagttgg tgagccgggt
     4201 cgattcgacg atcgtcctga gcgagctgca cgagcgtcgg cgggtcgatc acaaagcgcg
     4261 tcatgcggtc ggcggtacga gaccgcccga ggcggactgt cgcagtcgaa tagcgatcat
     4321 cttttcgacc agctcgcggg gcaggggctg gtcgatggcg
//
LOCUS       DATCBJ010000163         3223 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_205513, whole genome shotgun sequence.
ACCESSION   DATCBJ010000163 DATCBJ010000000
VERSION     DATCBJ010000163.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 3223)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3223)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3223
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_205513"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            539..1081
                     /locus_tag="VII50_03350"
     CDS             539..1081
                     /locus_tag="VII50_03350"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEY5049910.1"
                     /translation="MNRLFILRHAKAATHPPTASGGDHERPVAARGAAVLPRVARAIV
                     NELEGHPLDLVLCSTSTRTRETVELVAQAEPAVAAAPLKLDGRLYLADPARLLKTLRK
                     VPDGAATVLLCGHNPGLHQLALLLLGDDGSENAPEHVPARLRYDLPTAGLVVVDLDGS
                     WTSMGIGAAKLRAFVVPSDA"
     gene            complement(970..1698)
                     /locus_tag="VII50_03355"
     CDS             complement(970..1698)
                     /locus_tag="VII50_03355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5049911.1"
                     /translation="MGAWRDVVDDATSRLGPVSPALVRHWRAELDEVERRVALLECSA
                     PVGSAADGLHAFVALATARLADTWDPAQLIDVATSVELAHRASQHHRRVVDTTMADGE
                     RCPNARVVLDGDWSITQAAVLVADIGPAAYRCLVRGYGATQVGQIFGDAEPLALMKAA
                     VDLAALVAGVPDDVARGLWAAATRSPAAGARPASLPAAVVAWAVRSYASDGTTNARNF
                     AAPMPIDVQLPSRSTTTSPAVGRS"
     gene            1766..2599
                     /locus_tag="VII50_03360"
     CDS             1766..2599
                     /locus_tag="VII50_03360"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEY5049912.1"
                     /translation="MSFGVGAAAAHVIESPGASMGTDRAPTEVNTFARSLFADLPSRY
                     DTLAYLLSFGQDRRWRAAVVERITAAEPRRVLDVACGPAGVTLAIAHACDAEMVGVDL
                     TEDMIRQGLRNVRAAGLDDRIKLAIARAEEMPFPDGTFDAISFSYLMRYVEDPAATIA
                     ELARCLAPGGPIASLEFHVPGWAPARVVWWFYTRFVLPAAGGLTGGRAWYRVGRFLGP
                     NISYHYLRYPVAAHVANWRAAGLENVKVKPMSFGGGLVMSATRRREKSAGVVADVGRQ
                     V"
     gene            2599..>3223
                     /locus_tag="VII50_03365"
     CDS             2599..>3223
                     /locus_tag="VII50_03365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5049913.1"
                     /translation="MADTRRAPAFYGAGRGDGGWRDWITLLHPPYTAWHLSYVAIGAA
                     LAHPIRLDRLGWSLLGFFLGVGIGAHALDELHGRPLRTGVPSWALATVGGAAVAGAAV
                     DGWFVGGVHLLPFVAVGALLVLAYNLEWFGGAVHNSAGFAASWGAFPVLTGYYAQHWS
                     VSVGACLAAAGAFGLSFAQRTLSTQARWLRRSVREIDAHGTLNDGTPV"
BASE COUNT          475 a         1119 c         1108 g          521 t
ORIGIN      
        1 gaagcctggc tgcgtgcttc ggccatcgca ctgttgttcg tgttggcccg gtaggtgatg
       61 gcgagaacgc cgccgagcgc gacgacaggg accagcgatg ctgccgcgta gatcgcaaac
      121 aatgggacgg cgggcctgcg catatatgga atgtcgacct tcgccagccg gggagttcgg
      181 ccggatgcgc gtttcgacta caccggacgg cgcagcccgg cgcagccaat agggcctggc
      241 cgcaggtaca cggtgcttac cggtaaacgg tgcttaccgg taaacggtgc ttacaggtgc
      301 acggtgctta cgggtgtggt gctacccgaa tcgggagctg gtcgcctctc ggcgagaagc
      361 tcggcgcggc ttcggcgaac cgatccctcc gcgccggctg taggtgaggc ccggggacac
      421 gggctcccga tccgggtagc ggtctattca tcggcgacga gtaccccgaa ctgaacgccc
      481 caccggtggc ccagcaccac acgtagcaac agacgggcac acataccaga ctgtggcgat
      541 gaatcggctg ttcatcctgc ggcacgcgaa agcggcgacc catccgccga cggcgagcgg
      601 tggcgatcac gagcgacctg tcgcagctcg aggcgcagcg gtcctgccgc gcgttgcgcg
      661 cgccatcgtc aacgagctcg aggggcatcc gctcgatctc gtgctctgct cgacgtccac
      721 ccgcacccgc gagaccgtcg agctcgtcgc gcaggccgag ccggcggtgg ctgccgcacc
      781 gctgaagctg gacggccggc tgtaccttgc cgatccagct cgactgttga agacgctgcg
      841 caaggtccct gacggcgccg ccaccgtgct gctgtgcggc cacaaccccg gccttcatca
      901 gctcgccctg ttgctgctcg gcgacgacgg atccgagaac gccccagaac atgtaccagc
      961 cagactgcgt tacgacctgc cgaccgccgg gctcgttgtg gttgacctcg acggcagttg
     1021 gacgtcgatg ggcatcggtg cggcaaagtt gcgggcgttc gtcgtcccat cggacgcgta
     1081 ggacctgacc gcccaggcga cgacggcggc cggcaaggat gccggacgcg caccggcagc
     1141 gggtgaccgg gtcgctgcgg cccacaggcc gcgggccaca tcgtcgggca ctcccgccac
     1201 cagcgcagcg agatcgaccg ctgctttcat gagcgccaac ggttcggcgt ccccgaaaat
     1261 ctgaccgacc tgggtcgcgc cgtagccgcg cacgaggcac cggtaggccg cgggcccgat
     1321 gtcggcaacc agcaccgcgg cttgggtgat cgaccaatcg ccgtcgagaa cgacgcgcgc
     1381 gttcgggcac ctctcgccgt cggccatcgt ggtgtcgacc acccgccgat gatgctgcga
     1441 tgcgcggtgc gcgagttcga ccgacgtcgc cacgtcgatg agttgcgcgg gatcccacgt
     1501 atcggcaagc cgcgcggtgg cgagcgcaac gaacgcatgc agcccatcgg cggccgagcc
     1561 gactggcgcc gagcactcaa ggagcgcgac ccggcgttcg acctcgtcca gctcggcacg
     1621 ccaatgccgc acgagggcgg gcgacaccgg gcctagccga ctggtcgcgt cgtcgacgac
     1681 atcacgccac gcccccacgc cacctcccct acgctggctc ccggtctcgc aagttgtcca
     1741 tcggcgtcgg cgtagccgcc cacgcatgtc gttcggcgtc ggcgccgccg ccgcccatgt
     1801 gattgagtct cctggagcga gtatgggcac agatcgagcg ccaacggagg tcaacacctt
     1861 cgcgcgttcg ttgtttgcgg atctaccttc ccgctacgac acgttggcat acctgctttc
     1921 gttcggtcag gaccggcggt ggcgggccgc ggtagtcgag cgaatcacgg ccgcggagcc
     1981 tcgacgggtg ctcgacgtcg cgtgcggacc ggccggggtg acactggcca ttgcacacgc
     2041 gtgcgatgcc gagatggtcg gcgtcgacct gactgaggac atgatccggc aaggtctgcg
     2101 caacgtccga gccgccggtc tggacgaccg gatcaagctg gccatcgcgc gcgccgagga
     2161 aatgcctttc cctgacggca ctttcgacgc gatctccttc agctacctga tgcgttacgt
     2221 cgaggatccc gcggcgacca tcgccgaact cgcacgctgc ctggccccgg gcgggcccat
     2281 cgctagcctc gaattccacg tacccggatg ggccccggcc cgcgtcgtgt ggtggttcta
     2341 cacccgcttc gtgctgccgg cggccggcgg actcaccggc ggccgagcct ggtatcgcgt
     2401 cggccgcttc ctcggtccca acatcagcta ccactacctg cgctacccgg tggccgccca
     2461 cgtcgcgaac tggcgtgctg ccgggctcga gaacgtcaaa gtgaagccga tgagcttcgg
     2521 cggcggcttg gtgatgtcgg cgactcggcg acgtgaaaag tcggccggcg tggtggcaga
     2581 cgtgggtcgg caggtgtagt ggcggacacg cggcgggctc ccgccttcta cggcgccggg
     2641 cgcggggacg gcggttggcg cgactggatc acgctgctcc acccgccgta caccgcctgg
     2701 cacctgtcct acgtcgcgat cggcgcggcg cttgcccacc cgatccgcct cgatcggctc
     2761 ggttggagcc tgctcgggtt cttcctcggc gttggaatcg gcgcccacgc gctcgacgag
     2821 ctgcacggac ggccgctgcg caccggcgtc ccgagttggg ctcttgccac cgtcggagga
     2881 gccgccgtcg cgggcgccgc ggtcgacggg tggttcgtcg ggggcgtcca cctactgcct
     2941 ttcgttgccg tcggcgccct gctcgtgctg gcgtacaacc tggagtggtt cggcggggcc
     3001 gtgcacaaca gcgccggctt cgcggcgtcc tggggtgcgt ttccggtgct gaccggctac
     3061 tacgcgcagc actggtccgt ctcggtgggt gcctgccttg cggcggcggg tgcgttcggc
     3121 ttgtcgttcg cgcaacgcac gttgagtacg caagctcggt ggctgcgccg ctcggtgcgc
     3181 gagatcgacg cccacggcac cctcaacgac gggacgccgg tcg
//
LOCUS       DATCBJ010000164         4086 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_503124, whole genome shotgun sequence.
ACCESSION   DATCBJ010000164 DATCBJ010000000
VERSION     DATCBJ010000164.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 4086)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4086)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4086
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_503124"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(66..2612)
                     /locus_tag="VII50_03370"
     CDS             complement(66..2612)
                     /locus_tag="VII50_03370"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEY5049914.1"
                     /translation="MLRATLRNVFAHKVRLLLTGLSVVLGVGFLAGTLVFTDTLKATF
                     NDLIGRTSVNLSVVVRAKSDFSSTDIGASSERALVPNSLVATVAAVPGVRQAIGEVQG
                     IDLLVTEAGKAVQPKSPGPPTLAVSWTPANFGSLAFVQGAGPTGPGQVAVDQSAADSY
                     HLHVGDHVTVQTQGTPIQATIVGIVTVGGSSNLAGAVLSVFDPVTAAQVAGKPGFANQ
                     IDVQAASGVSQEALAARIGPTLPKGFEAVTAKAVNKEQSTAISKALGFFNVFLLIFAG
                     VALFVGLFIILNTFTMLVAQRTRELALLRAIGASRRQVMAAVLGEALVVGVVASTVGL
                     VVGLLVAVGVRGLLDAVGVSMPAGSLVIKAHTIIASYIVGVLVTLLAAWFPSFRASRI
                     PPVAAMRDGVALPERSLRIRAVIGGVLTVLGAFAIAAALGNGTGGAAAQIGIGILLVM
                     VGIWVMSALISRPVVKVIGAPLVWIFGTPAQLGRSNAVRNPRRTAATSASVMIGLALV
                     TMLTVLAASTKSSITSVVNNNLGADYVLTSQSFLGFSPDVATKAIATPGVATVGETRV
                     GAAHLNGKTVVLAAVSTDIGSVIKVKMASGSLNALEKNELLISKTQATTSKLQLGDVV
                     SVEYPTGGMQHIDVGGIFDDNSLLGNGGADYLIGLATYAKNYTTQLDAIVYVLAKPGQ
                     REAVGAALESSLKAYPQVKVQGQSAYKDSITKQIDQFVNLIFGLLALALIIAVLGIIN
                     TLALSVYERTREIGLLRAVGMTRRQLRRMIRLESAVIAVFGAVLGLLLGVIFGWAVVS
                     NAGGQLNHVVFPIGSFLSFVIGAGVVGVLAALWPAWRASRRPVLAAIATE"
     gene            complement(2612..3394)
                     /locus_tag="VII50_03375"
     CDS             complement(2612..3394)
                     /locus_tag="VII50_03375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY5049915.1"
                     /translation="MSEPTISEPLSDVVTAAAARALHLSKIYGEGETTVAALRDVTIE
                     FERGHFSAIMGPSGSGKSTLMHCLAGLDSVTSGSAIIGDVDLGTLSDKALTLLRRQAI
                     GFIFQSFNLIPTLTALENITLPMDIAGRKPDQGWLDAVIDAVGLRDRLHHKPSELSGG
                     QQQRVACARALASRPTIIFADEPTGNLDSRASAEVLGLLQRSVREMSQTIVMVTHDPW
                     AAAYADRVVFLADGTVIDQMRDPTQAAVLERMSRLEARAQEA"
     gene            complement(3478..>4086)
                     /locus_tag="VII50_03380"
     CDS             complement(3478..>4086)
                     /locus_tag="VII50_03380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HEY5049916.1"
                     /translation="ADPSAGSDEAISAWLASARVLLETYFRLEDPTRVEPAQRELLVE
                     AELADGVRLRGFVDRVDVAPSGATRIVDYKTGNSPSELFEAKALFQLKFYALVMWRVS
                     RTIPALLQLMYLGDGQILRYAPDEADLLATERKVRALWAAIERALANREFPARRGRLC
                     DWCAHQSLCPEFGGVTPAYPEPTGAASVVPLDQPTEADDITP"
BASE COUNT          740 a         1331 c         1338 g          677 t
ORIGIN      
        1 tccgcccgtc tgggccatat cggccgagcc ggggcccgat cgtgcgggcg tggcaggcgg
       61 cgggtctact cggtggcgat cgcggccagg actggacgcc gggaagcgcg ccatgcgggc
      121 cacagcgccg ccaggacgcc gaccacgccg gcaccgatga cgaacgacag gaacgatccg
      181 atcgggaaga ccacgtggtt caactggccg cccgcgttcg acacgaccgc ccatccgaag
      241 atcactccga gcagcaaccc aaggaccgcg ccgaagaccg cgatgactgc ggattcgagc
      301 cggatcatcc ggcgcagctg ccgtcgtgtc atgccgacgg cccgcagcag cccgatttcc
      361 cgggtgcgct cgtagaccga caacgcgagg gtgttgatga tgcccaggac ggcgatgatc
      421 agtgccagcg cgagcagtcc gaagatgagg ttgacgaact ggtcgatctg cttggtgatc
      481 gaatccttgt acgcgctctg gccttggacc ttgacctgcg gataagcctt caggctcgac
      541 tcgagagcag ctccgacggc ttcgcgctga ccgggtttgg cgagcacgta cacgatggca
      601 tcaagttggg tggtgtagtt cttggcgtat gtcgcgagtc cgatcaggta gtcggcacca
      661 ccgttgccca agaggctgtt gtcgtcgaag atcccaccga cgtcgatgtg ctgcatgcca
      721 cccgtcgggt attcgacact gacgacgtca cccaactgca acttgctcgt cgtcgcctga
      781 gtcttcgaga tgagcagctc gttcttttcc aatgcattca gggatccgct ggccattttc
      841 actttgatga ccgagccgat gtcggtcgac accgcggcga gcacaacagt cttgccgttc
      901 aagtgcgcgg cgccgactct ggtctcgccc acggtcgcca cacccggcgt cgcgatcgcc
      961 ttggtcgcga catcggggga gaaccccagg aacgactgcg acgtcagcac atagtcggca
     1021 ccgaggttgt tgttgactac tgacgtgatc gaggacttcg tcgacgctgc cagtacggtc
     1081 agcatggtga ccagcgcaag cccgatcatc acggacgccg aggtggccgc ggtccggcgc
     1141 ggattgcgga cggcgttgga gcgcccgagt tgggccgggg ttccgaagat ccacacgagg
     1201 ggtgcaccaa taaccttgac gaccggtcgg ctgatcagtg cactcatcac ccagatgccg
     1261 accatcacga gcaggatgcc gatgccgatt tgggcggccg caccgccggt gccgttgccg
     1321 agtgccgctg cgatcgcaaa cgctccgagg actgtcagca cgccgccgat cacagcgcga
     1381 atgcgcagcg acctttcggg cagcgcgaca ccgtcgcgca tggcggcgac cggagggatt
     1441 cgagatgcgc ggaaggacgg gaaccacgcg gcaagcaggg tgacgagcac gccgacgatg
     1501 taggacgcga tgatcgtgtg cgccttgatg acaagcgagc cagccggcat cgacactcct
     1561 acggcatcga gcaagccccg cacaccgact gccacgagta ggccgacaac caggccgacc
     1621 gttgatgcga cgacgccgac gaccagcgcc tcgcccagca ccgcggccat cacttgtcga
     1681 cggctcgcgc cgatggctcg caacagcgcg agttcacgag tgcgttgcgc taccagcatg
     1741 gtgaaggtgt tgaggatgat gaacaacccg acgaagagcg caacacccgc gaagatcagc
     1801 aggaagacgt tgaaaaagcc gagcgccttg ctgatcgctg tggattgctc cttgttcaca
     1861 gccttcgcgg tgactgcctc aaagcctttc ggcagagtcg ggccgatccg cgcggccagc
     1921 gcctcttgcg agacgccgct cgcagcttgg acgtcgatct ggttcgcgaa gccgggcttg
     1981 cctgcgacct gcgcggcggt taccggatcg aacacactca gcaccgcacc tgccaggttg
     2041 ctgctgccgc cgacagtgac gatgcccacg atggtggcct ggatcggagt tccctgggtc
     2101 tgcaccgtga cgtggtcgcc gacgtgcagg tggtaggaat cggccgcact ctggtcgacc
     2161 gcaacctggc cgggtccggt ggggccggcg ccctgcacga aggcgaggct gccgaaattg
     2221 gcgggagtcc acgacacagc gagcgttggc gggcccggcg atttaggttg cacggccttt
     2281 cccgcttcag tgacgagcaa gtcaatgccc tgcacctcac ctatggcttg ccgaacgcct
     2341 ggaaccgccg cgaccgtcgc gaccaacgag ttcggtacca gcgcgcgctc gctgctggca
     2401 ccgatatccg tgctggagaa gtctgacttc gcgcgaacga caacgctgag gttgacactc
     2461 gtgcgcccga tcaggtcgtt gaacgtcgct ttcaacgtgt cggtgaacac gagcgttcct
     2521 gccaagaatc cgacgcccag gaccaccgac aggccggtca gcaggagtcg taccttgtgg
     2581 gcgaagacgt tgcgcagggt cgctcgcagc atcaggcttc ctgagctctc gcttcgagcc
     2641 gactcatccg ctcgagcacc gccgcctgcg tcggatcgcg catctggtcg atgacagtgc
     2701 cgtccgcgag gaacacgaca cggtcggcgt atgccgcggc ccaagggtcg tgcgtcacca
     2761 tgacgatggt ctggctcatc tcgcgcaccg aacgctgcag cagccccagc acttcggcac
     2821 tcgcgcgcga gtcgaggttc ccggtcggtt cgtcggcgaa gattatggtg ggccgcgacg
     2881 caagcgcacg cgcgcacgcg actcgttgct gctgaccacc ggagagctcg gacggcttgt
     2941 ggtgcaggcg gtcgcgcagg ccaaccgcgt cgatcaccgc gtcgagccac ccttgatcgg
     3001 gcttgcggcc ggcgatgtcc atgggcagcg tgatgttctc gagcgcggtc aacgtcggga
     3061 tcagattgaa cgactggaag atgaacccga tcgcttggcg ccgcagcagc gtcagcgcct
     3121 tgtcgctcag ggtgccgagg tcaacgtcgc cgatgatggc gcttcccgag gtcacgctgt
     3181 cgaggcctgc cagacaatgc atcagcgtcg acttgcccga cccggacggt cccatgatcg
     3241 cgctgaagtg cccgcgctcg aactcgatgg tgacgtcgcg taacgcggca accgtcgttt
     3301 ctccctcgcc atagatcttg gacaggtgca gcgctcgggc cgccgctgct gtgacgacgt
     3361 cgctcagtgg ttcggaaatc gttggttcgg acatgtgagc cacgctacct gcgacccata
     3421 gcccgacgcc tcggacggac cgcaccgatc aacggtgaca accgtcatgg cgagggccta
     3481 cggcgtgatg tcgtcggcct cggtgggttg gtcgagcggg acgaccgatg cagcgccggt
     3541 cggctcgggg taggcagggg tgacgccacc gaactccggg cacagcgact gatgcgcaca
     3601 ccaatcgcag agccggccgc gccgcgccgg aaactcccgg ttcgccagcg ctcgctcgat
     3661 cgcggcccac aacgccctga ccttgcgctc ggtggcgagc aagtcagcct cgtctggtgc
     3721 gtaacgcagg atctgcccgt cgccgaggta catcagctgg agtagcgccg ggatcgtgcg
     3781 gctgacacgc cacataacca gggcgtagaa cttcaactgg aacaaggctt tggcttcaaa
     3841 gagctccgac ggcgagttgc ccgtcttgta gtcgacgatg cgggtcgccc cgctgggcgc
     3901 aacatcgaca cggtcgacga agcccctcag ccgcacaccg tcggccagtt cggcttcgac
     3961 cagcaactca cgctgcgccg gctcgacccg ggtcgggtct tcgagccgga agtaggtctc
     4021 gagcaagacg cgcgcgcttg ccagccaggc gctgatggcc tcgtcggagc ccgcagacgg
     4081 gtcggc
//
LOCUS       DATCBJ010000165         4173 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_58622, whole genome shotgun sequence.
ACCESSION   DATCBJ010000165 DATCBJ010000000
VERSION     DATCBJ010000165.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 4173)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4173)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4173
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_58622"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..979)
                     /locus_tag="VII50_03385"
     CDS             complement(<1..979)
                     /locus_tag="VII50_03385"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF013180.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HEY5049917.1"
                     /translation="MAGTGHRAPEVAVTALWGAPAGSVEFEPALGIPWQSGLDPLTGL
                     ADRRWFLRAVAVADGSASGAADDVGIIAVRMIGLRSVLVSHGRVAADGVLLLAAEVLH
                     GWSRGDDLAARLGVDSFGILAHTDAAGLAATAARLRTKLADAGVSATIGSAMRAAQGG
                     ASEALRTTNRDVGKSNGTRRADEAAASRAIRGASAGTAAQVIQAQATGILMQWHRCGA
                     DRARLELAYQAHEMGIPVTSMARLLVAVASGAPLSDQDAAYGIDLERSTRLAANVRAG
                     LASRTSTIRGERWAPEGARDDSAYTPSRHTQPVWSVRPPNSASGLLSLAG"
     gene            complement(1098..1799)
                     /locus_tag="VII50_03390"
     CDS             complement(1098..1799)
                     /locus_tag="VII50_03390"
                     /inference="COORDINATES: protein motif:HMM:NF013736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF and ANTAR domain-containing protein"
                     /protein_id="HEY5049918.1"
                     /translation="MRAAIEDVLVGGERGAPAADRLCVACLPLLGVDAAAVSLIDEGQ
                     SRGTFGASDAAARLLDSLQFTAGEGPCLEAAQTMVPVLVPDLHAQDEPRWLAFQDAAE
                     RSGVRAVFALPIRVGMIGLGALDLFRFTAGPLSAEQLTDALLAADAAAQILLDLIDES
                     STATARGDPLAQWAQVASLSRAEVYQATGMIMAQLDLPPAAALVRLRAYAFANELTAS
                     EVAQLVTSRHLRMEA"
     gene            complement(2166..2939)
                     /locus_tag="VII50_03395"
     CDS             complement(2166..2939)
                     /locus_tag="VII50_03395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009077494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF and ANTAR domain-containing protein"
                     /protein_id="HEY5049919.1"
                     /translation="MSNDDAGGGPSPVRDREQLLAETFVVLADTLVDDYDVVDLLHQL
                     VDACVNLLGVTAAGLLLDDQRGNLAVVASSNEETRLLEVLQLQSDAGPCLDCVRTGVP
                     VSVDDLDADRARWPVFAAAAISAGFRSVAAVPLRLRTETIGALNMFHRAPYPVAVNDR
                     RLAQALADVATIGILQRRSSHRSAMMAEQLQHALNSRIVIEQAKGVLAERNAVDMDAA
                     FTALRRYARNHNRKLSELAMDVVRGQVDPTAVQTGRRVT"
     gene            complement(2936..3616)
                     /locus_tag="VII50_03400"
     CDS             complement(2936..3616)
                     /locus_tag="VII50_03400"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF and ANTAR domain-containing protein"
                     /protein_id="HEY5049920.1"
                     /translation="MDDSGAEDDSVPRRICLLCVRMLGITGAGISMVTTAGNRGVVCA
                     TDDVSARIEDLQLTLGEGPCVDAAQFGTPILVPDLTTPDDIAVDHWPAFIEGAGAAGV
                     RAVFAFPLRIGAINVGVLDVYRSEPGPLRDGQLRAGLLAADAAALALLHIGTASDDLF
                     ADNTDTRSTYQIQVHQATGVVQVQLGVPTEEAFLMLRARAFALGRPLVDVATDVVARR
                     LRFSVEDL"
     gene            complement(3764..>4173)
                     /locus_tag="VII50_03405"
     CDS             complement(3764..>4173)
                     /locus_tag="VII50_03405"
                     /inference="COORDINATES: protein motif:HMM:NF012575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Rieske (2Fe-2S) protein"
                     /protein_id="HEY5049921.1"
                     /translation="PPRLRRRRRRPRHCRARQRQRQRRHPPRSQPKPAGRKIGPASAV
                     LVAGSAAFQDPATGGPAIVVQPTAGTFLGFDAACPHAGCTVAYDQGRVQFACPCHGLQ
                     FSGTTGAVEVGPASHGLRRITVAEGSDGQLYAR"
BASE COUNT          779 a         1434 c         1326 g          634 t
ORIGIN      
        1 cgcccgccaa cgaaagaagc ccgctcgccg agttcggggg tcgaacgctc cagactggct
       61 gcgtgtgccg gctcggtgtg tacgcgctgt cgtccctcgc accctcaggc gcccaccgtt
      121 cgccccggat ggtcgacgtt cgtgacgcca gacctgccct gacgttggcg gccaaccgcg
      181 tcgagcgttc aagatcgatc ccgtacgcag cgtcctggtc actgagaggc gcacctgaag
      241 caaccgcgac gagtaagcga gccatcgacg tgaccgggat gcccatctcg tgggcctggt
      301 aagcgagctc gagacgtgcg cgatcagccc cacagcgatg ccactgcatc aagatgcccg
      361 tcgcctgcgc ctggatcacc tgggcagcgg tcccggcgct cgcgccacgg atggctcgcg
      421 atgccgccgc ctcgtccgcg cgtctggtgc cgttgctttt gcccacgtcg cggttcgtgg
      481 tccgcaacgc ctctgacgct ccgccctgcg cggcgcgcat ggctgacccg atcgtcgctg
      541 atacccccgc gtcggcgagc ttcgtccgca gccgcgccgc ggtcgcggcc aggcccgcag
      601 cgtccgtatg cgcgaggatg ccgaaactgt cgacccccag tcgggcggcg aggtcgtccc
      661 cccggctcca cccgtgcagc acctctgcgg ccagcaacaa gacaccgtcg gcggccaccc
      721 gaccatgact caccagcacg ctccgcagcc cgatcatgcg aaccgcgatg atgcccacgt
      781 catccgcggc gccacttgcc gaaccatccg ctacggcgac ggcacgcagg aaccaccggc
      841 gatcggccaa cccggtgagc ggatcaagac cactttgcca aggaataccc agcgctggtt
      901 cgaactcgac ggagcccgcc ggcgcccccc acaacgcagt gacagcaacc tccggcgcac
      961 ggtgccctgt gccagccaca gcagcccacc ctccgcagaa agctgatgcg ggatgcgttg
     1021 ccaggtttct ggagcaacac gctcgacgcc ggttggcgcc gagtcgtacc gggttcaaac
     1081 ctcgctatct ctggcagtca cgcctccatc cgcagatggc gcgacgtgac gagttgagcg
     1141 acctcagacg cggtgagttc gttcgcgaac gcgtaggccc gtaaccgaac gagcgccgcc
     1201 gcaggcggca ggtcaagttg ggccatgatc atcccagtcg cctggtagac ctcggcccgt
     1261 gacaatgacg cgacttgcgc ccattgcgcc agcggatccc cccgcgccgt tgccgtgctc
     1321 gactcatcga tgagatcgag caggatctgt gcggccgcat cggcggccag gagcgcatcg
     1381 gtcagttgct cagccgacag cggacccgct gtaaagcgga acaaatcgag tgccccaagt
     1441 ccgatcatgc ccacccgaat cgggagggcg aaaacggccc gaactccgga ccgttccgcg
     1501 gcatcctgga acgcgagcca tcgaggttca tcctgagcat ggaggtccgg gaccagcacc
     1561 ggcaccatcg tctgtgctgc ctcgaggcac ggtccctcac ccgcagtgaa ctgcagggaa
     1621 tcgagcagtc tcgcagcggc gtcactcgca ccgaatgtgc cgcgactttg cccttcatcg
     1681 ataagcgaaa ctgccgcggc atcgactccg agcagtggca ggcacgccac acacaatcga
     1741 tccgccgcag gtgcgcctcg ctcaccgccg accaggacat cctcgatggc cgccctcatc
     1801 gtcgactcat cggttgcgcg acagcgacgt tgcagatgca gtcgcgctgc aactcgttgg
     1861 acgtcgttgc ccggcagtac cgcagtgcgg tcctgtcgtg gcgagccgcc acgtgtccgc
     1921 agtcgcacgc tgcgtccgga gagggcgaaa ggtcgacgag ttgggaggag aggaaggcag
     1981 acggttcgtc gatcagccgc tcggtcatgc cgctcgccgc cttcggtcag aggctgacgc
     2041 gccgaggggc aaggtccaga cgaacccaag cacgaagaac tagccccatg atcgcacaaa
     2101 agcgtcaaac aatggtaaga agcgaccctg ccgcgttatc ggcagcagcc gtcggtcgga
     2161 tcgcgctagg tgacccggcg tccagtctgg acggcggtgg ggtcgacctg accgcggacg
     2221 acgtccatcg cgagctcact gagcttgcga ttgtgattgc gcgcgtagcg tcgcaacgcc
     2281 gtgaaggcgg cgtccatgtc gacggcgttt cgctcggcga gcacgccctt agcctgctcg
     2341 atcacgatcc ggctattgag tgcatgttgg agttgctcgg ccatcatcgc gctgcgatgg
     2401 ctggagcgcc tttgcaagat gccgatcgtt gccacgtcgg ctagcgcctg ggccagccgt
     2461 cgatcgttca cggccaccgg gtacggcgcc cggtgaaaca tgttcagagc accaatggtc
     2521 tctgtgcgaa ggcgcagcgg gaccgcggcc accgaccgga acccggccga gatggcggca
     2581 gcggcgaaca ccggccagcg ggcccggtcg gcgtccagat catcgacgct gaccgggaca
     2641 ccggtccgca cgcaatctag gcagggcccc gcgtcgcttt gcagctggag gacctcaagc
     2701 aaacgggttt cttcgttcga cgaggcgaca acggcgagat tgccgcgttg gtcatcgagc
     2761 agcagtccag ccgcagtcac gccgagcagg ttgacgcagg catcaaccag ttggtgcagg
     2821 agatcgacga cgtcgtagtc atcgacaaga gtgtccgcaa gcaccacgaa cgtctccgcg
     2881 agcaactgct cgcgatctct gaccggactc ggtccgccgc ccgcatcatc gttactcata
     2941 gatcctccac cgaaaaacga agacgacgcg cgacgacatc cgtcgccacg tcaacgagcg
     3001 gtcgaccaag ggcgaacgca cgcgcgcgaa gcatcaggaa cgcttcctcc gttgggacac
     3061 caagctgaac ttgcaccacg ccggtcgcct gatgcacttg gatctggtag gtcgagcggg
     3121 tgtcggtgtt atcagcgaac aggtcatcgc tggcggtacc gatgtgcagc agtgccaacg
     3181 cggccgcgtc ggcagcaagc agcccggcac gcaattgacc atcacgcaag ggcccgggtt
     3241 cgctgcggta gacgtcgagg acaccgacgt tgatcgcccc gatccgcagc gggaacgcga
     3301 ataccgcacg cacgccagcg gctccggcac cctcgatgaa tgccggccag tgatcgacag
     3361 caatatcgtc aggggtggtg aggtcgggga ccaagatggg tgtaccgaac tgcgcagcat
     3421 cgacacacgg gccctcgccg agagtgagtt gaagatcctc gatgcgcgcc gaaacatcgt
     3481 cggtggcaca cacaacgcca cggttgcccg ctgtggtcac catggagata cccgcgccgg
     3541 tgatgccaag cattcggaca cacagtaggc agatccggcg tgggacggaa tcgtcttccg
     3601 cacccgagtc atccaactag cgtgcccgcc catccaagtc atccgttccc ccaggcaccg
     3661 gacaaaggtg tccagcctga cctaagcatg caccagtcat gaatcgaacg cgagtatcgc
     3721 ggccagccaa aaccacgtga tcgtgacgat atcgaccccc tcgctaccgg gcgtagagct
     3781 gtccgtccga cccctccgcg accgtgatgc gccgcaggcc atggcttgcc ggaccaacct
     3841 cgaccgcgcc agtggtcccg ctgaactgca aaccgtggca cggacaggcg aactggacgc
     3901 ggccttggtc atacgcgacg gtgcagccgg catgcgggca ggcggcgtcg aaaccgagaa
     3961 aggtgccggc cgtgggttgc acgacaatcg ccgggccgcc ggtcgccgga tcctgaaagg
     4021 ccgcagaacc agccaccagg acggcgctgg cgggcccgat cttgcggccg gccggcttcg
     4081 gctggcttcg cggtggatgc cttcgctgtc gctggcgttg gcgtgcccgg caatggcgtg
     4141 gccggcgtcg gcgccgtcga agccggggag gac
//
LOCUS       DATCBJ010000166         2932 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_500307, whole genome shotgun sequence.
ACCESSION   DATCBJ010000166 DATCBJ010000000
VERSION     DATCBJ010000166.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2932)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2932)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2932
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_500307"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..742)
                     /gene="efeU"
                     /locus_tag="VII50_03410"
     CDS             complement(<1..742)
                     /gene="efeU"
                     /locus_tag="VII50_03410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033573 - high-affinity iron permease
                     complex [Evidence IEA];
                     GO_function: GO:0005381 - iron ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034755 - iron ion transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake transporter permease EfeU"
                     /protein_id="HEY5049922.1"
                     /translation="MLPTFVIGLREGLEASLIVGIVAAFLGRQGRRDALRQVWIGVGV
                     AVAICLAVGIALQIVSSDLPQRQQDGLETVVGLLAVAMVTYMVLWMRKHSRDLKGQLE
                     GAAAAALAQGSARALVVMAFLAVLREGFETAVFLLATFRASGNAAASATGAVLGIAVA
                     VAIGYGIYRGGVRINLSRFFRATGVVLVVVAAGLVMTAFHTANEAGWLTVGQVQAFDL
                     SWLIHPGSALSAVLTGVLGLQPYPVWIDV"
     gene            complement(892..1884)
                     /locus_tag="VII50_03415"
     CDS             complement(892..1884)
                     /locus_tag="VII50_03415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009083648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEY5049923.1"
                     /translation="MSAELLQRARSGDREAFSELVEPHRRELQVHCYRMLGSLQDAED
                     ALQDSLLAAWLGLDGFEGRSSVRTWLYRIATNRCLNLLRSSSRRPVTAAPLPIVPPEP
                     TRLGEVLWLQPYPDALLEGMSDDAPGPEARYESREAISLAFVTVVQLLPPNQRAVLLL
                     RDVLGYRASETAELLGLTEEAVTSALKRARATMDAAGSPSLPPPTPDSPQERALLDRF
                     VAAFTSLDVDSLVALMTDDAWVRMPPLPFEYRGTAAVHAFFVATAPHRREIAQLVPVR
                     ANGQPAWGEYRRDSTTGGLHLMGIEVVGIAGDRICEITRFETTVAPHFGLPRTL"
     gene            2016..2759
                     /locus_tag="VII50_03420"
     CDS             2016..2759
                     /locus_tag="VII50_03420"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEY5049924.1"
                     /translation="MTQASRRAGAAPTAILHSAVLVTGANRGIGYALVEEALRRGARR
                     VYAGTRQPFTHPDERVTPLTLDVTSAAQIRAAVEKVDALDILINNAGVAAYDDLSDRA
                     VLEQHLAVNLFGTYSVTQAFLPLLTRSQGVIVNALSMTALAPYPIIPAYSISKAAALS
                     LSQSLRALLAVRGIRVHAVLTGPTDTDMTRGLDIPKASASSVAQAILDGVENGDEEIF
                     PDPMSASIADDWGTGAAKTLERQNAAMAS"
BASE COUNT          523 a         1014 c          958 g          437 t
ORIGIN      
        1 caacgtcgat ccacaccgga tagggctgca ggccaaggac gccggtgagc acggccgaca
       61 gtgcgctgcc gggatgaatc agccacgaca ggtcaaacgc ctgcacctgt ccgacggtga
      121 gccagccggc ttcgttcgcc gtgtgaaacg cggtcatgac cagtcccgct gcaaccacga
      181 ccagcaccac gccggtcgcc cgaaagaacc gcgacaggtt gatccggaca ccgccgcggt
      241 agatgccgta gccgatggcg accgcaacag cgatgccgag cactgccccg gtggcgctcg
      301 ccgccgcgtt cccgctcgcg cgaaaggtgg cgagcaggaa gaccgctgtt tcgaagcctt
      361 cgcggaggac cgcaaggaag gccatcacga ccagtgcgcg tgccgaccct tgcgccagcg
      421 ccgcggcggc tgcgccttcg agttgaccct tcaggtcacg cgagtgcttg cgcatccaca
      481 agaccatgta ggtgaccatc gcgaccgcga gcaggccgac gacggtctcg agaccgtctt
      541 gctgacgttg cgggaggtcg ctcgacacga tctgcagcgc gataccgacg gcgaggcaga
      601 tcgcgacagc gacgcccact ccgatccaca cctggcgcag cgcgtcgcgg cgaccctgcc
      661 ggcccaggaa ggcggcgacg atgccaacga tcagcgatgc ctcgaggcct tcgcgcagcc
      721 cgatgacaaa ggtcgggagc acgctgcctc cggggcgagg tgtggatagg gttacctaac
      781 taaggggacc ctaactcacg tcggcagctc cgtgcaagaa ccggcgcccc cgtccgctgc
      841 cctgccgggg cggtgggctc aagggattgt cgcaacgcct gagctgaggg gctacagggt
      901 tcggggcagg ccgaagtgtg gcgcaaccgt cgtctcgaac cgggtgatct cacagatccg
      961 gtcgccggct atgccgacca cctcgatgcc catgaggtgc aggccgcctg tcgtcgaatc
     1021 gcggcggtac tcaccccacg cgggctggcc gttggcccgg accggcacca gctgcgcgat
     1081 ctcgcgccgg tgtggcgcag tggcgacgaa gaaagcgtgc accgccgccg tcccgcggta
     1141 ttcgaatggc aacggcggca tccgcaccca cgcgtcgtcc gtcatcaacg cgaccagcga
     1201 atcgacgtcc aacgacgtga aggctgcaac gaagcgatcg agcagcgcac gctcctgcgg
     1261 actgtccggc gttgggggtg gcaggctcgg cgaaccagcc gcatccatcg tcgcgcgggc
     1321 gcgcttgagt gcgctcgtga ccgcctcctc ggtgagcccg agcagttccg cggtctcact
     1381 cgcgcggtat ccgagcacgt cgcgcagcag cagcacggcg cgctgattcg gcggcagcag
     1441 ttggacgacg gtcacgaacg ccagcgagat cgcctcgcgt gattcgtagc gggcctcagg
     1501 accgggcgca tcgtcggaca tcccctcgag cagcgcgtcg ggatatggct gcagccacag
     1561 cacttcgccc aggcgcgtcg gctccggcgg cacgatcggc agcggcgccg cggtcaccgg
     1621 ccgtcgcgac gacgaccgga gcaggttcag acaccggttg gtggcgatgc gatacagcca
     1681 ggtgcgcacc gacgagcgac cttcgaaacc gtcgagtccg agccacgccg caagcaacga
     1741 gtcctgcagc gcgtcctcgg cgtcttgcag cgagccgagc atgcggtagc agtgcacctg
     1801 cagctcgcgg cggtgcggtt cgaccagctc gctgaaagcc tcgcggtcac ctgaacgcgc
     1861 gcgctgcagc agttctgccg acatccttga cctccgactc catcatctcc ccgtactgac
     1921 accggcggcc acccgaactg gtcgcacgtg gggcgcccag tttcggccgc ctgccttgtc
     1981 tattccggtg accacacacg aacagggaga acgaaatgac acaagcatca cgcagggcgg
     2041 gcgccgcgcc caccgcaatc ctgcacagcg ctgttctggt aacgggcgcg aatcgcggca
     2101 tcgggtatgc gctggtcgaa gaggctttgc gtcgcggtgc gcggcgcgtg tacgccggca
     2161 cgcggcagcc cttcacgcac ccggacgagc gggtcacgcc gctgacgctc gacgtgactt
     2221 ctgcggctca gattcgagcg gccgtcgaga aggtcgacgc gttggacatc ttgatcaaca
     2281 acgccggcgt cgccgcctac gacgacctca gcgaccgggc tgttctcgag cagcaccttg
     2341 cggtgaatct cttcggcaca tacagcgtca cgcaggcgtt cctgccattg ctgacgcgct
     2401 cgcagggggt catcgtcaac gcgctgtcca tgaccgcact cgcgccttac ccgatcatcc
     2461 cggcgtactc gatctccaag gcggcagcgt tgtcgctctc gcagtcgctg cgcgctctgc
     2521 tcgccgtccg cggcattcgg gtgcacgccg ttctcactgg tcctactgac accgacatga
     2581 ctcgagggct cgacatcccc aaggcgtctg cgtcgtcggt ggcgcaagcg atcttggacg
     2641 gcgtggagaa cggtgacgag gagatcttcc ccgaccccat gtcggcgtcg atcgcggatg
     2701 actggggtac cggcgcggcc aagacactcg agcgccagaa tgcggcgatg gcgtcatgag
     2761 cgactacgcg acccggttca cggtctccca aagtcccgac gaggtattcg ctgccatcaa
     2821 cgacgttcgc ggatggtggt caggcgacat cgaaggcagc accgacgaac tcggcgccga
     2881 gttcacctac cgctaccgcg acgtccaccg cagcacgcag aagatcaccg ag
//
LOCUS       DATCBJ010000167         2813 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_205131, whole genome shotgun sequence.
ACCESSION   DATCBJ010000167 DATCBJ010000000
VERSION     DATCBJ010000167.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2813)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2813)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2813
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_205131"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(160..573)
                     /locus_tag="VII50_03425"
     CDS             complement(160..573)
                     /locus_tag="VII50_03425"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF023395.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HEY5049925.1"
                     /translation="MDPSCIFCAIAAGTAPSYLTYEDDTAVAFLDLAPLRQGHTLVIP
                     RRHVPDVMSDEGAQALIDIGPALHHVGRRLMEVFDADGINLLQANRAAAGQVVFHLHV
                     HLVPRHEGDRSPLRWERDPQAADAVAQTHALIAES"
     gene            complement(578..970)
                     /locus_tag="VII50_03430"
     CDS             complement(578..970)
                     /locus_tag="VII50_03430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019514492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2237 domain-containing protein"
                     /protein_id="HEY5049926.1"
                     /translation="MFPNLNVLGGDLEPCGNDPITGFFRDGCCSTGSEDLGSHTVCAV
                     MTGPFLEHQRAIGNDLITPMPAYEFPGLEPGDRWCVVAVRWFEAYEAGVAAPVVLAAT
                     HARALDVIPLDALREHAVDVPSDPSSLA"
     gene            complement(992..1120)
                     /locus_tag="VII50_03435"
     CDS             complement(992..1120)
                     /locus_tag="VII50_03435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5049927.1"
                     /translation="MGSKEKGGREIRKPKKDAKVKGVPAGSGVVSAQTQAKDRPAK"
     gene            1223..2068
                     /locus_tag="VII50_03440"
     CDS             1223..2068
                     /locus_tag="VII50_03440"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEY5049928.1"
                     /translation="MPRVVVTGGSGKLGQACVADLLAHGWDVVNVDRMPSPHPVCPFL
                     RVDLTDFGQTVEALSVNDGYRGVDAVVHLAAIPAPGLMTNAATFANNITSTYNVFAAA
                     RHSGISNIVWASSETVLGLPFEIPPPYIPVDEEYPARPESTYSLVKHLEEQMARQFCR
                     WDPDLKMIGLRFSNVMDPTDYVAFPGYDADPNLRKWNLWGYIDARDGAQAVRRALDHE
                     VVGADVFIIAAADTVMTRSNAELTTEIFPDVEVRGEIGEHETLLSIGKARRVLGFEPQ
                     FGWRT"
     gene            complement(2065..>2813)
                     /locus_tag="VII50_03445"
     CDS             complement(2065..>2813)
                     /locus_tag="VII50_03445"
                     /inference="COORDINATES: protein motif:HMM:NF037610.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sterol carrier protein domain-containing
                     protein"
                     /protein_id="HEY5049929.1"
                     /translation="TGYALMPALFDGFRRAQSGAIRRDNVDFDREFGKGTVPGRKPWV
                     GWVAVHRPSPGADIDGLVRYHVDERWEGMRPMSVAVIDDLVSTNSVAYAALWRLCCDL
                     DNIVTVTAPNRTIDESLPWMLADARAMVQTGRFDNVWLRILDAEMALPARRYLTTGAL
                     VIEVRDAMNHAAGRYLLEAGPDDASCTRTTTAADIELDVTSLGAAYLGGTSLQTMAAA
                     GLVVEHRAGAVALADAMFRWTAVPWCNTWF"
BASE COUNT          471 a          921 c          945 g          476 t
ORIGIN      
        1 gagccgacag tgaccgacga cgtcggcgcg aaagatgcgg tggcgggcgt gctggcggtg
       61 gtcgcagtct tcttgctgcg tgcggagcag ccgcccgtga gcgcgggcac gactgatagg
      121 ccggcggcgg ccgcaccgac gtcctttccg tgcatgcgct cagctctcag ctatcaacgc
      181 gtgtgtctgc gccacggcat cggcggcctg cggatcccgt tcccagcgca gcggcgatcg
      241 gtcgccctca tgacggggta ccaggtggac gtggaggtgg aacacgacct gaccggcggc
      301 agcgcggttc gcctggagca ggttgatccc gtcagcgtcg aagacctcca tgagccgtcg
      361 acccacgtgg tggagggccg ggccgatatc gatcaacgct tgcgcgccct catcggacat
      421 cacgtccggc acgtgccggc gaggaatgac caacgtgtgg ccttgccgca gtggcgccag
      481 atcgagaaac gcgactgccg tgtcgtcttc gtaagtcagg tacgaaggcg cggtgcccgc
      541 cgcgatcgcg cagaagatgc aggaggggtc catgccgtca cgccaaagaa ctgggatcgc
      601 tcgggacgtc gacggcatgc tcgcgcagcg cgtcgagcgg aatgacgtca agagctcgag
      661 catgcgtcgc ggcgaggacg acaggtgccg cgacgccggc ttcgtaggct tcgaaccatc
      721 gcacggcgac gacgcaccac cggtcgccgg gttctaggcc ggggaactcg taggcgggca
      781 tcggtgtgat caggtcgttg ccgatagccc gttgatgctc aaggaatggc ccggtcatga
      841 cggcgcagac cgtatgactg ccgagatcct cggacccggt cgagcaacag ccgtcgcgaa
      901 agaatcccgt gatggggtcg ttgccgcacg gctcgaggtc accgccgagc acgttgagat
      961 tgggaaacat gacgcgtgcg gaccgctcga gctatttcgc tggacggtcc ttggcctggg
     1021 tctgcgcaga tacgactccg ctgccggcgg gcacgccctt gaccttggca tccttcttcg
     1081 gcttgcggat ctcgcggccg cccttttcct tgctgcccat cacggactcc ctcggttcgt
     1141 cgcggttggt ggtccgccgg ggttgggtgc gcctcggcgc cgtcggccta ggctatcgac
     1201 attacgtcga ctagggagtc ccatgccacg tgttgttgtc accggcggaa gcgggaagct
     1261 cggccaggcg tgtgtcgctg acctgttggc tcacgggtgg gacgtcgtga acgtcgaccg
     1321 gatgccgtcg ccgcaccccg tgtgcccgtt cctgcgggtc gacctgaccg acttcggcca
     1381 gaccgtcgaa gcgctgtcgg tcaacgacgg ctatcgcgga gtcgacgccg tcgtccatct
     1441 cgcagcgatt cctgcccccg gcctcatgac gaacgcggcc acgttcgcga acaacatcac
     1501 ctcgacgtac aacgtgttcg ccgccgcgcg ccactccggc atcagcaaca tcgtctgggc
     1561 gtcgagcgag accgtactgg gcttgccgtt cgagatcccg ccgccgtaca tccccgtcga
     1621 cgaggagtac cccgcgcggc ccgagtcgac gtactcactc gtgaagcatc tcgaggagca
     1681 gatggccagg cagttctgcc ggtgggaccc cgaccttaag atgatcgggt tgcgtttctc
     1741 caacgtgatg gacccaaccg actacgtcgc gtttccgggc tacgacgccg atccgaatct
     1801 gcgcaagtgg aacctgtggg gttacatcga cgcccgcgac ggagcgcagg cagtgcgccg
     1861 cgccctcgac cacgaggtcg tcggggcgga cgtgttcatc atcgcggccg ccgatacggt
     1921 gatgacgcgt tcgaacgctg agctcactac cgagatcttt ccggacgtcg aggtgcgcgg
     1981 cgagatcggc gagcacgaga ccttgttgtc gatcggcaag gcacgacgcg tgctcgggtt
     2041 cgaaccccag ttcggctggc gcacctagaa ccaggtgttg caccacggaa cggccgtcca
     2101 ccgaaacatc gcatcggcca gcgcgacagc accggcacga tgctcgacaa ccaggcccgc
     2161 ggccgccatg gtctgcagcg acgttccgcc gaggtaggca gcgccgaggg acgtgacatc
     2221 aagctcgata tcggccgccg tggtcgtgcg cgtgcagctc gcgtcgtccg gccctgcttc
     2281 aagcaggtac cgaccggcgg cgtggttcat cgcatcgcgc acttcgatga ccagggcgcc
     2341 ggtcgtgagg tagcgccgcg cgggcagtgc catctcggcg tcgaggatgc gcagccagac
     2401 attgtcgaag cgtccggtct gcaccatcgc gcgagcgtcg gcgagcatcc agggtagcga
     2461 ttcgtcgatg gtgcgattcg gcgccgtgac ggtgacgatg ttgtcgaggt cacagcacaa
     2521 cctccacaag gcggcgtacg ccaccgagtt ggtcgacacg agatcgtcga ttacggcgac
     2581 gctcatcggg cgcatgccct cccagcgctc gtcgacgtgg tagcgcacca acccgtcgat
     2641 gtctgcacca ggcgacggtc gatggaccgc gacccaaccc acccacggct tccgtcccgg
     2701 caccgtgcct ttgccgaatt cgcggtcgaa gtcgacgttg tcgcgcctga tcgcgcctga
     2761 ctgcgcgcga cggaaaccat cgaaaagcgc gggcatgagt gcgtagccgg tcg
//
LOCUS       DATCBJ010000168         2648 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_168489, whole genome shotgun sequence.
ACCESSION   DATCBJ010000168 DATCBJ010000000
VERSION     DATCBJ010000168.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2648)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2648)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2648
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_168489"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            73..420
                     /locus_tag="VII50_03450"
     CDS             73..420
                     /locus_tag="VII50_03450"
                     /inference="COORDINATES: protein motif:HMM:NF015735.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HEY5049930.1"
                     /translation="MAQYLILIYESEEGYANASPDDLGATMQAHTRFAEQIPALGGTM
                     LGGNALQPTTTATSIRDDIVTDGPFVETKEALGGYYLIEAADLDQALAISKLCPAAFG
                     GVEVRPIMDLPGG"
     gene            417..1673
                     /locus_tag="VII50_03455"
     CDS             417..1673
                     /locus_tag="VII50_03455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009078220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEY5049931.1"
                     /translation="MTTPSDEVHRAIADAHRREWAFVLAATARVAGDIDLAEECVQDA
                     YVSALDAWTRDGIPSNPAAWLTTAARHRAVDIHRRHQTLRGKLPLLVEPDVAPADVED
                     ADLIADDRLRLVFTCCHPALGREAQVALTLRLVCGLTTAEIAQVFLVPEPTMAARVTR
                     AKKKIALAHIPYRVPDSAELPDRLDAVLTVVHLLYTTGHTAPAGEQLVRADLVDRSVG
                     LARMLLVLMPDEREVRGLLALILLTDARRRTRVDATGRLLLLEEQDRTQWDRTQIAEG
                     VALVINALRGGGAGRFAVQAAIAAVHAEAPSYDETDWPQVLELYDELLRRWPSPVVAL
                     NRCVAVLMVDGAKAALDEVDALDASGRLAGYRYLPATRADLLRRLERFTEAAEAYRSA
                     MALADNERERTFLAGRLSEVLELSRV"
     gene            complement(1670..>2648)
                     /locus_tag="VII50_03460"
     CDS             complement(1670..>2648)
                     /locus_tag="VII50_03460"
                     /inference="COORDINATES: protein motif:HMM:NF028473.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5049932.1"
                     /translation="AVVTPDGVSHSAKNFAIVSESTGAVLYAGNANSYVRAITSLNGV
                     TYVGGDFTSFGGASRSHAAALSSSFAVTAWNPTPGNTVRALAAAGSTIYLGGDFSSVR
                     AVSSSTGSTIWSKSTSTGHVHSLLATNGRLYAGGLFQTYNGTTQHGLVEINPSNGNLV
                     TTFNAHLRPDTHVGQYGAYDGEEIISLSVGPDSGEILAGSGGHAPAGDSSNEAILLNA
                     TTGARVWTTSLIGDCQGIGLVGSTVVAGYHRNNSNTTTPFPYFATQLELSNGRLTTWD
                     PKITGNQSNADGGNNGVQAIYVDQSTNTIYLAGAFTSPSSHKSLIAFTF"
BASE COUNT          442 a          835 c          904 g          467 t
ORIGIN      
        1 gattccacgt ggtgccattc gtcataggga tgtcaagctc ttagtggccg aaccgatcgc
       61 ggaggaatac ccatggcgca atacctgatc ctgatctacg agagtgaaga gggatacgcg
      121 aacgcttctc ccgacgacct cggtgccacg atgcaggcgc acacgcggtt cgcggagcag
      181 atcccggcac tgggcggcac catgctcggc ggcaacgccc tgcagccgac cacgaccgcg
      241 acgtcgatcc gcgacgacat cgtgacggac ggtccgttcg tggagacgaa ggaggcgctt
      301 ggtggctact acctgattga ggcagcagac ctcgaccagg ccctcgccat ttccaagctc
      361 tgcccagcag cgtttggcgg cgtcgaagtt cgtccgatca tggatctccc cggcgggtga
      421 ccactccgtc cgacgaggta catcgggcaa tcgccgacgc gcatcgtcgc gagtgggcct
      481 tcgtgctcgc cgcgacggcg cgcgtcgccg gcgatatcga ccttgccgag gagtgcgtgc
      541 aggacgccta tgtgtcagcg ctcgatgcct ggacccgtga cggcatcccg agcaacccgg
      601 ccgcatggct gacgaccgcg gctcgccatc gtgcggtgga catccaccgt cggcaccaaa
      661 ccctgcgcgg gaagctgccg ctgctcgtcg aacccgacgt cgccccggcc gacgtggagg
      721 acgctgatct gatcgctgac gaccggctgc gattggtgtt cacctgctgt cacccggcgc
      781 ttggccggga ggcacaggtc gcgttgacgc tgcggctggt ctgtggtctg accacagctg
      841 agatcgcgca ggtattcctg gtgccggagc cgacgatggc tgcccgcgtg acacgggcga
      901 agaagaagat cgcgttggcg cacattcctt accgcgtgcc ggattcggcg gagctgcccg
      961 accgtctcga tgcggtgctg accgttgtcc atctcctcta cacgacaggg cacaccgcgc
     1021 cggcggggga gcagctcgtc cgcgccgatc tggtcgatcg gtcggtcggg ttggcgcgga
     1081 tgctgcttgt cctcatgccc gacgagcgag aggtgcgagg cctgcttgcc ctgatcctct
     1141 tgaccgacgc gcgtcgacgc acccgcgtcg atgcaacggg ccggctgctg ttgctcgagg
     1201 agcaagatcg cacgcaatgg gaccgcacgc agatcgccga gggagtggcg ctcgtcatca
     1261 acgccttgcg cggcggggga gccggccgct tcgcggtgca ggcggcaatt gcggctgtcc
     1321 acgcggaggc tccgagctac gacgagaccg actggccgca ggtgctcgag ctctacgacg
     1381 agctccttcg tcggtggccg tctcccgtgg tcgccctgaa ccggtgcgtc gccgtcttga
     1441 tggtcgacgg ggcaaaagcc gcactcgatg aggttgatgc gcttgacgcg tcggggcgac
     1501 tcgccggcta tcgatatctg ccggccaccc gtgccgactt gttgcggcgg ctcgaacgat
     1561 tcaccgaagc cgccgaggcc taccgatcgg ccatggcact cgccgacaac gaacgggagc
     1621 gcacattttt ggccggcagg ctgagcgagg tcttggagct cagccgagtc tagaaggtga
     1681 atgcgatcaa agacttgtgg ctcgatgggc tggtaaacgc gccagccaag tagatcgtgt
     1741 tcgtgctctg gtcgacatag atggcctgca cgccattgtt gccgccgtcc gcgttcgact
     1801 gattaccggt gatctttggg tcccacgtcg tcagccgccc gttcgacaat tccaactggg
     1861 tggcgaagta cgggaacggc gtcgtggtgt tggagttgtt gcggtggtag cccgcgacga
     1921 cggtggagcc gaccaggccg ataccttggc aatcgccgat gagcgacgtc gtccacacgc
     1981 gggcgccggt cgtggcgttc agcaagatgg cctcgttcga cgaatcgccg gcaggcgcgt
     2041 gtccaccact gccggccagg atctcgccac tgtcggggcc gacggacagc gagatgattt
     2101 cctcgccgtc gtatgcgccg tactgaccaa cgtgggtgtc gggccgaagg tgcgcgttga
     2161 acgtggtcac caggttgcca ttgctcgggt tgatctcgac gagcccgtgc tgggtggtgc
     2221 cgttgtacgt ctggaacaga ccgccggcgt acaaccttcc gttggtggcg agcagggagt
     2281 gcacgtggcc ggtgctggtg ctcttcgacc aaatcgtgga cccggtcgag gacgacaccg
     2341 cgcgaacgga actgaagtcg ccgcccaggt agatcgtgct gcccgcagcg gccagtgcgc
     2401 gaacggtgtt gcccggcgtc gggttccatg cggtcacggc gaacgacgag gagagcgccg
     2461 ctgcatgcga gcggctcgca ccaccgaacg aggtgaagtc gccgccgacg taggtgacgc
     2521 cgttcaggct agtgatggcg cgaacatacg aattcgcgtt gccggcgtag agcacggcgc
     2581 cggtcgactc cgacacgatc gcgaaattct tcgcactgtg cgaaacgccg tcaggggtta
     2641 cgaccgcg
//
LOCUS       DATCBJ010000169         2558 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_402992, whole genome shotgun sequence.
ACCESSION   DATCBJ010000169 DATCBJ010000000
VERSION     DATCBJ010000169.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2558)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2558)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2558
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_402992"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..492)
                     /locus_tag="VII50_03465"
     CDS             complement(<1..492)
                     /locus_tag="VII50_03465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HEY5049933.1"
                     /translation="MSAQTEELLADLNPQQRAAVVHEGGPLLIVAGAGSGKTRVLTRR
                     IAYLLAERHVAPPEILAITFTNKAAGEMKERVAAMVGNRAKVMWVMTFHSACVRILRE
                     NAKRLGFTTTFSIYDSADSHRLMALVCRELDLDPKRYPPRAFNAQVSNLKNELVDSED
                     FERR"
     gene            552..1289
                     /locus_tag="VII50_03470"
     CDS             552..1289
                     /locus_tag="VII50_03470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L deacetylase family protein"
                     /protein_id="HEY5049934.1"
                     /translation="MPVEPLPDAEIERVLVITAHPDDVDFGSAGTIATFTDAGIEVVY
                     CICTDGDAGGYDLEFPRDQIAAVRRAEQTEAAKRVGVHDVRFLGYPDGALVPSFDLRR
                     DISRVIRQVQPQRALVQSPERDYTRVGRSHPDHRAAGDAALDALYPDARNPFAYPELR
                     LDEGLDAWVVKEIWVMGGVDGRDLHYVDVTDTFDRKISALKAHESQTAHMDNLEEFVG
                     RWLTANAEAAGLPVGRKAESFLVMATP"
     gene            1430..2377
                     /locus_tag="VII50_03475"
     CDS             1430..2377
                     /locus_tag="VII50_03475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5049935.1"
                     /translation="MSSPFGPTPGAGEPTADELVTGAPKARRGTTTAIALITGGAVVG
                     GILASTLGASASSNASSTTASAVTSAVSSSAPAPSSTAPGTKKVRPAFVAPLTGKVTA
                     VGTDNVTIQTTATSSTASTTLTYSVTSTTRISINESGPGALTGVKVGDTVFFNVTTTG
                     GTVLADLRDGTGPAGGGFGPGGFGGRGPGGPGFGGVIGVAPETGTVAAVGTDTIGIKT
                     STATTTYSLASTGHVEKPGTTKATLTDVKVGDTVFFTVTTKGGTVIANLFDGQLPTFA
                     GHAGAGGPGFGGPGFGGRMGHGPKAPAASPAASASGTST"
     gene            complement(2379..>2558)
                     /locus_tag="VII50_03480"
     CDS             complement(2379..>2558)
                     /locus_tag="VII50_03480"
                     /inference="COORDINATES: protein motif:HMM:NF039724.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6314 family protein"
                     /protein_id="HEY5049936.1"
                     /translation="GKFFYRADLSTGSDTFVHGCAPDSYCGSWSVDSADRFHLTWDVE
                     GPAKQITIRSTYSRA"
BASE COUNT          460 a          879 c          822 g          397 t
ORIGIN      
        1 gcgacgctcg aagtcttctg agtcgaccag ctcgttcttc agattcgaga cctgcgcgtt
       61 gaaggcacgc ggcggatagc gcttcgggtc gagatcgagc tcgcggcaca ccaacgccat
      121 caaccggtga gagtcggcgg agtcgtagat ggagaacgtg gtggtgaagc cgagccgctt
      181 ggcattctcg cgcaggatgc gcacgcacgc cgagtggaag gtcatcaccc acatgacctt
      241 cgcccggttg ccgaccatcg ccgcaacccg ctctttcatc tcgccggcgg ccttgttggt
      301 gaaagtgatc gccagaatct cgggcggcgc gacgtgccgc tccgcgagca gataggcgat
      361 gcgacgggtc aagactcgcg tcttgccgga gccggcgccc gcgacgatga gcagcggacc
      421 cccctcgtgc acgacggccg cgcgctgctg ggggttgaga tcggcaagga gctcttcggt
      481 ctgcgctgac atcggccttg agtctacgga gccgccggga caagcagggc caagcgccta
      541 gactggcgct tgtgccagtc gagcccttgc cggacgccga gatcgagcgc gtcctcgtca
      601 tcaccgccca ccccgacgat gtcgattttg gatcagcggg gacgatcgcg acgttcaccg
      661 acgccggtat cgaggtcgtc tattgcatct gcaccgacgg cgacgccggc gggtatgacc
      721 ttgagttccc gcgtgaccag atcgccgccg ttcgccgagc cgagcagaca gaggcggcca
      781 agcgggtcgg cgttcacgat gttcgttttc ttggctaccc cgacggcgct ctggtgccga
      841 gcttcgattt gcgtcgtgac atcagccggg tgatccgcca ggtgcagcca cagcgcgccc
      901 tggtacaaag ccccgagcgc gactacacga gggtcgggcg cagccacccc gaccaccgcg
      961 ccgccggtga cgcagcactc gacgcgctct accccgacgc acgcaacccg ttcgcctatc
     1021 ccgagctgcg gcttgacgag ggcctcgacg cctgggtggt taaggagatc tgggtcatgg
     1081 gcggggtcga cggccgcgac ctgcattatg tcgacgtaac cgacacgttc gaccgcaaga
     1141 tctcggcact caaggcccac gagagccaga cagcacacat ggacaatctc gaagaattcg
     1201 ttggccgctg gctaacggcg aacgcggaag ctgccggctt gccggtaggc cgaaaggctg
     1261 aatccttcct ggtcatggcc actccctgag gctgcgggag cgcaaatccg agcagcttga
     1321 gtgcttgctg tcagctattt cccagcttgg cgaccgattg tcaatagaca cgggggcagc
     1381 cggttgggca gtccgagaca gcagtcccag acaagggaga tccgcgcata tgagttcacc
     1441 tttcggaccg acgcctggcg ccggcgagcc aacggctgac gaactcgtca ccggcgcgcc
     1501 aaaagcccgc cgcggcacga ccaccgccat cgccctgatc accggcgggg cagttgtcgg
     1561 aggcatcctc gccagcacgc tcggtgcgtc ggcctccagc aacgcgtcct cgacaacagc
     1621 gtccgcagtg acatccgctg tgtcgagctc tgctcccgca ccttccagca cggcccccgg
     1681 caccaagaag gttcgccccg cgttcgtcgc accgctcacc ggcaaggtca ccgccgtcgg
     1741 cacggacaac gtcacgatcc agacgactgc caccagcagc acagcctcga ccaccctgac
     1801 gtacagcgtg acgagcacca cccggatctc gatcaacgag agcggcccgg gagcactgac
     1861 gggcgtgaag gtcggcgaca cggtcttctt caacgtcacg acgaccggcg gcacggttct
     1921 cgctgatctg cgcgatggca ccggcccagc cggcggcggc ttcggccctg gcggcttcgg
     1981 cggccgcggc cccggtggtc ctgggttcgg cggcgtcata ggcgtggcgc ccgagaccgg
     2041 cactgtcgcc gctgttggca ccgacacgat cggcatcaag accagcaccg cgacgaccac
     2101 ctacagcctg gcgagcaccg gccacgtcga gaagcccggc accacaaagg caacgctgac
     2161 cgacgtgaag gtcggtgaca ccgtcttctt caccgtcacg accaagggcg gcacggtcat
     2221 cgccaacctg ttcgacggcc agctgccgac gttcgccggc cacgccggcg ccggagggcc
     2281 agggttcggc gggccagggt tcggcggccg tatgggtcac ggaccgaagg ctcccgcagc
     2341 atcgccggcc gcgtcggcaa gcggcaccag cacctgagtt acgccctgct gtaagtcgac
     2401 ctgatcgtga tctgtttcgc cggcccctcg acgtcccacg tcaggtgaaa tcggtccgct
     2461 gaatcgacgc tccacgaacc gcagtagctg tcgggcgcgc agccatgaac gaaggtgtcg
     2521 ctgcccgtcg aaagatcggc gcggtagaag aacttgcc
//
LOCUS       DATCBJ010000170         2115 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_168359, whole genome shotgun sequence.
ACCESSION   DATCBJ010000170 DATCBJ010000000
VERSION     DATCBJ010000170.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2115)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2115)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2115
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_168359"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..402)
                     /locus_tag="VII50_03485"
     CDS             complement(<1..402)
                     /locus_tag="VII50_03485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5049937.1"
                     /translation="MAAELPSDQAPAATPPSGDAPAARRPWYRRMRRPRYVGIWGATA
                     VLFAFSPLLAANSLTSSALLGMLPLAAMLAIASIGQTLVVQQRGLDLTVPGMILLTAV
                     IVTLHANQSNGRLGGAVLLVVLACVASGLVSG"
     gene            complement(778..>2115)
                     /locus_tag="VII50_03490"
     CDS             complement(778..>2115)
                     /locus_tag="VII50_03490"
                     /inference="COORDINATES: protein motif:HMM:NF012602.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HEY5049938.1"
                     /translation="HLKLRPATDSMLAMGMMRVLIENDWVDHEFIDKHTIGYDALRDT
                     VMAVSIDEVAGITGIPADQVREVAALYHEHRPGLIQVGQGLQRNLNGGEIVLAVCMLA
                     AIAGQVGVPGGGVFYSNYEWNFKDISHPEFRTEGTRLYNMIKLGQWLTANDDIQSLYV
                     YNQNPAVTCPNQTLVRRGMLRDDLFVVVHDLFMTDSAKLANVVLPATTFAEHSDLHYS
                     YWHDYVQINNKIIEPAGEARSNFATFRSIALAMGYTDPCFHQEEEEVIAEALEGTGMA
                     MADLIEGPVFNGEFDRTSFDDGRFGTASGKVELLAPTFTDYGDEKYRYRLLSPKTKLM
                     HGSQAGNLPRMAPRLGEPYFYIHPTDAEREQIDDGAPVRVYNDRGAVVLAARVSERTQ
                     PGVIVSYNGRWGDVNVNVTTSDAEADMGGQAIFQSNWVDLELAAQHNGSHVDA"
BASE COUNT          335 a          675 c          745 g          360 t
ORIGIN      
        1 cccgctcacg agccccgatg cgacacaggc cagcacgacg aggaggacgg caccgccaag
       61 gcgcccgttc gattgattcg cgtgcagcgt gacgatgacg gcggtgagca ggatcatccc
      121 ggggacggtc agatccaacc cacgctgctg caccacgagg gtctggccga tcgacgcgat
      181 cgcgagcatc gcggccagcg gcagcatccc gagcagggcg ctgctggtca ggctgttcgc
      241 cgcaagcagc gggctgaacg cgaacagcac ggctgtcgct ccccagatgc cgacgtagcg
      301 tggtcgccgc atgcggcgat accaggggcg gcgggcggcc ggggcgtcgc cggaaggggg
      361 cgtcgcggcg ggcgcctggt cgctcggcag ctcggcagcc acgtgcgccg atcacctccc
      421 gaggagtgtt gacgctggca ccggcaggca cggccgcagc gccaggagca ggggctcgcc
      481 gcccgtttga ccggagagca cggctgcggc tcggctaact gccggcgccg cggcgcggcg
      541 gcgccgggcg cgggtccggt agagcgtgaa tcgcggcggc gagctcatcg agcgtcgccc
      601 cgtgcgtcgc tcggccgagc acgaagcaca gcaggggagc gttccgtcgg tcaccgcttt
      661 catgcgcggc gacacctgcg agctcaagca gcagcgccgc ggtcggtgac gtcgagctcg
      721 ccggagggaa ggccggcgca caccgcggcc gtgtcgcgcg cgtgcgacag ccacgcgtca
      781 agcgtcgaca tgtgacccgt tgtgctgcgc agccagctcc aggtcgaccc agttggattg
      841 gaagatggcc tgcccaccca tgtcggcctc tgcgtcggac gtggtcacgt tcacgttcac
      901 gtcgccccag cggccgttgt aggagacgat gacgcccggc tgagtgcgct ccgacacgcg
      961 tgcggcaaga acgactgctc cgcggtcgtt gtagacgcgg accggcgcac cgtcgtcgat
     1021 ctgctcgcgt tcggcgtccg ttgggtggat gtagaagtac ggctcgccaa ggcggggcgc
     1081 cattcgcggg aggtttcccg cctggctgcc gtgcatcagc ttcgtcttcg gcgacagcag
     1141 ccggtagcgg tacttctcgt cgccgtagtc ggtgaaggtc ggagccagca gctcgacctt
     1201 gccgctggcc gtgccaaacc gcccgtcgtc gaagctcgtg cggtcgaact cgccgttgaa
     1261 cactgggcct tcgatgaggt ccgccatcgc catgcccgtg ccctcgagag cttcggctat
     1321 gacctcttcc tcctcctggt ggaagcaggg atcggtgtag cccatcgcga gagcgatcga
     1381 gcggaaggtc gcgaagttcg agcgcgcttc gccggccggc tcgatgatct tgttgttgat
     1441 ctgcacgtag tcgtgccagt aggagtagtg aagatcggag tgctcggcga atgtcgtcgc
     1501 cggaagcacg acgttcgcga gctttgcgga atccgtcatg aacaggtcgt ggacgacgac
     1561 gaacagatca tcgcgcagca tgccgcggcg gacgagcgtc tgatttgggc acgtcacggc
     1621 tgggttctgg ttgtagacgt acagcgactg gatgtcgtcg ttggctgtga gccactgccc
     1681 gagcttgatc atgttgtaga ggcgcgttcc ctcggtgcgg aactcgggat ggctgatgtc
     1741 cttgaagttc cactcgtagt tcgaatagaa cacgccgccg ccggggaccc cgacctgtcc
     1801 ggcgatggcg gcgagcatgc aaaccgcgag cacgatctct ccgccgttga ggttgcgctg
     1861 aagtccctga ccgacctgaa tcagaccggg cctgtgttcg tggtacagcg ccgcgacctc
     1921 gcgtacctgg tcggcaggga tcccggtgat ccccgcaacc tcgtcgatcg acaccgccat
     1981 gacggtgtcg cggagcgcgt cgtagccgat cgtgtgcttg tcgatgaact cgtggtcgac
     2041 ccagtcgttc tcgatcagga cgcgcatcat ccccatcgcg agcattgagt cggtcgctgg
     2101 gcggagtttg aggtg
//
LOCUS       DATCBJ010000171         2257 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_20072, whole genome shotgun sequence.
ACCESSION   DATCBJ010000171 DATCBJ010000000
VERSION     DATCBJ010000171.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2257)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2257)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2257
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_20072"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..323
                     /locus_tag="VII50_03495"
     CDS             <1..323
                     /locus_tag="VII50_03495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009304567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methionine adenosyltransferase domain-containing
                     protein"
                     /protein_id="HEY5049939.1"
                     /translation="RWVAKHVVAAGLADRCEVQVAYAIGKAHPVGLFVETFGTGLVPD
                     DRIQEAVVQVFDLRPGAIIRDLDLLRPIYRQTAAYGHFGRPELDFTWESIGRADALRA
                     AAGR"
     gene            416..>2257
                     /locus_tag="VII50_03500"
     CDS             416..>2257
                     /locus_tag="VII50_03500"
                     /inference="COORDINATES: protein motif:HMM:NF036997.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HEY5049940.1"
                     /translation="MTERRTTSSRPPRPRSRGVGAPGIRAPADVDPVARIAVEVSLPH
                     LDRPFDYLVPEPLAASVVTGSRVRVRFAGQLVDGFVLDRVAETDHTGRLAFVERSVSP
                     EVVLTPEIARLARATADRWAGTLADVLRLAVPPRHARAEARALDSAAALPVPAGDASA
                     WARYGSGRQFLTALRDGRSPRAVWPALPGPSWPAEIAELVATTLASGRGAVVVVPDGR
                     DVSRVDAALQARLGRDRHVVLTADLGPSERYRRFLLASRGAVDAVVGTRAAAFAPVHN
                     LGLVVVWDDGDDLHAEPRAPYPHVREVLVLRAHLASAAAVVGGHAVTAEAARLVATGW
                     AKLLAPSRVEVRASAPMAAPGDDPAAQRQPSAGGRLPTVAWEAAHAALGNQRPVLIQV
                     PRRGYLPALSCEGCRTPLRCPACAGLLASAGAGSAPTCRWCGRIAAGWEGPECGSRRY
                     RATVVGARRTAEELGRSFRGVLVRTSAQGQVLDTVPAGPALVIATPGAEPVADGGYGA
                     ALLLDGWALLQRADLRAGEEALRRWLNAAALVRPAADGGRVVVLADSSLRAVQALIRW
                     DPVGHAERELAERGVLGFPPAVTIAEVSGEAGAVDEVIVTTAMPPDAQ"
BASE COUNT          267 a          786 c          841 g          363 t
ORIGIN      
        1 tgcgctgggt cgccaagcac gtcgtcgctg ctggtctggc cgaccgttgc gaggtgcagg
       61 tcgcttacgc gatcggcaag gcgcacccgg tcgggctgtt cgtcgagacg ttcggcaccg
      121 gactcgtgcc cgacgatcgg atccaggaag ccgtcgtgca ggtctttgac ctgcgcccgg
      181 gcgccatcat ccgcgacctc gacctgctgc ggccgatcta ccgccagacc gctgcttacg
      241 gtcacttcgg tcggcccgaa ctcgacttca cctgggagtc gatcggccgc gccgacgcgc
      301 tgcgcgccgc ggccggtcgc tgattcgcgc agaggtgatg cgttgaccgc ggtgatccat
      361 agccgcggct cacgcccgca cggggtcact gccgtctggt aggactgcca gcgcgatgac
      421 agagcgccgc acgacatcca gcaggccgcc ccgacctcga tcacgtgggg tcggagctcc
      481 gggtatcagg gcgcctgccg acgttgaccc ggttgcccgg atcgccgtcg aggtctcgct
      541 gccccacctt gaccgaccct tcgactacct cgtgccagag ccgctggcgg catccgtcgt
      601 caccgggagt cgagtccggg tgcggtttgc cggccagctc gtcgacggct tcgtcctcga
      661 tcgggtggcc gagaccgacc acacgggccg cctcgcgttt gtcgaacgat cggtctcgcc
      721 cgaggtggtc ctgacgccag agatcgcccg cctggctcgc gcgaccgccg atcgttgggc
      781 aggcactctc gccgacgtgt tgcgtcttgc ggtgccgccc cgacacgccc gggcagaagc
      841 gcgtgcgctc gattcggcgg ccgcgctgcc cgtaccggct ggtgacgcgt cggcatgggc
      901 gcgctacggc tcgggtcggc aattcctcac cgcgctccgc gacgggcgat ccccgcgtgc
      961 cgtgtggcct gcgctgcccg gcccgagctg gccggcggag atcgctgagc tcgtggcgac
     1021 gacgctcgca agcgggcggg gggccgtggt cgtcgtcccc gatgggcgtg acgtgtccag
     1081 ggtcgacgcc gcgttgcaag cgcggcttgg gcgtgaccgc cacgttgttc tgacggccga
     1141 cctgggtccg tcggagcgct accggcgttt cctgctagcc agcaggggag cggtcgatgc
     1201 agtcgtcggt acccgggccg ccgcgtttgc gccggtgcac aacctcggtc tcgtagtcgt
     1261 ctgggacgac ggcgacgacc tgcacgcgga accacgagcg ccttatcccc acgtccgcga
     1321 ggtgctcgtg cttcgcgccc atctcgcgag cgcagcggcc gttgtcggcg ggcatgcggt
     1381 gacggcggaa gcggcccgac tggtggccac gggatgggcg aagctgctcg cgccgagccg
     1441 ggtcgaggtg cgcgcgagcg cacccatggc ggcgccgggc gatgacccgg ccgcacagcg
     1501 gcagccttcg gcggggggcc ggttgccgac cgttgcttgg gaggcggcgc acgcggcgct
     1561 gggcaaccag cgccccgtcc tcattcaggt cccacgtcgt ggttacctgc cggcgctttc
     1621 atgtgagggt tgccggacac cgcttcggtg cccggcctgc gcgggcctgc tggcgagtgc
     1681 gggtgccggc agcgcaccga cctgccgctg gtgtggccgg atcgcggccg gctgggaggg
     1741 tcccgaatgc ggcagccgcc ggtatcgagc gaccgtcgtc ggcgcgcgtc ggaccgcgga
     1801 agaactcggt cgatcgtttc gtggtgtcct cgtccgcacc tcggcccagg ggcaggtgct
     1861 cgacaccgtg ccggccggac cggctctggt gatcgcgacg ccgggcgccg agcccgtggc
     1921 cgacggcggt tacggggcgg ctctgctgct cgacggctgg gcgctgctac aacgagccga
     1981 cctgcgtgct ggcgaggaag cactgcggcg ctggctgaac gcggccgctc tggttcgccc
     2041 ggctgccgat ggcggccggg tggtggtgct cgccgacagc tcgctgcggg cggtgcaggc
     2101 cttgattcga tgggatccgg tcggccatgc cgagcgcgag ctcgcagaac gcggtgtgct
     2161 tggcttcccg ccggccgtga ccatcgccga ggtgtcgggt gaagctggcg cggtcgacga
     2221 agtcatcgtg acgacggcga tgccgcccga cgcgcag
//
LOCUS       DATCBJ010000172         4476 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_168209, whole genome shotgun sequence.
ACCESSION   DATCBJ010000172 DATCBJ010000000
VERSION     DATCBJ010000172.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 4476)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4476)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4476
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_168209"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(259..1467)
                     /locus_tag="VII50_03505"
     CDS             complement(259..1467)
                     /locus_tag="VII50_03505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5049941.1"
                     /translation="MNGRVSTAFAVLFLVAACGSGGSATSATNPPPSPTSAPLSVPPP
                     LAKTGSFPPWLTSAPASSYLSESSSATGVSLPADGEYMCVSKQIAICRDQGGEATATT
                     LTSGEDTDVTPTSGVTDYDPTLSVAAGTVYFRQTQNTATAAINTIHRVSVHGGPSTPI
                     ITAPQNNGDEGVGLGSPQISPDGRLLAYVITVGRTGLPSSAIPAPTGTLAPLGTNIFV
                     LNEGAGATAPLVVPSTVTGPELPDGFTGYRLVGWSPDDRQLYYFGGPTRELRALTFDA
                     TEHPISSSTVVQPSAPQSPCPDSPEASAMSPNGDVYFVAYCQETAVTIERVHNGQVFA
                     FASIPELTGGWITVDVHIDTTGRYLQLDAVPLDTHCLGGSAYVALLDGVVIAKQIQTN
                     PYGCPPGARP"
     gene            complement(1515..3251)
                     /locus_tag="VII50_03510"
     CDS             complement(1515..3251)
                     /locus_tag="VII50_03510"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEY5049942.1"
                     /translation="MDPHEQRPDAEPVLRETALLRRVRESVIGDDQVMPGPYGPRRVT
                     YADYTASGRALSFLEDFIRDEVLPRYANTHTESSGTGLQTTRLREDARRIIRDAVGGD
                     DETVVIFAGSGCTGAIDKLIGILGLRIPAELEDRYHLSDAIPPAQRPVVFIGPFEHHS
                     NELPWRESIVDVVVIPQDADGHIDASALEDELVRYNDRSVKIGSFSAASNVTGIVSDT
                     YAISALLHRHGALSFWDFAAAAPYVDINMYGGADPLAHKDAIFLSPHKFIGGPSTPGV
                     LVVRRELLHNRVPDVPGGGTVAYVNPTEHRYLTDPAQREEGGTPAIVESIRAGLVFQL
                     KQAVGIDVIRAHEEHYLRRAVTAWRAEPAIEILGDLDAERLSIVSFVVRAPSGRYLHH
                     NFVVALLNDLFGIQSRGGCSCAGPYGHRLLGIDLDRSHEFEREIAGGCEGIKPGWVRV
                     NFNYFISEPVFTYIVDAVRLVARDGWRLLTDYLFDPATGLWRHRRGLVEPPLRLSQVN
                     YEADGAMGYPHHDDRAAESELASYLAEAVSLFAASAGDDASEADRPSGALSEDFEHLR
                     WFDLPAQCLAVG"
     gene            3507..>4476
                     /locus_tag="VII50_03515"
     CDS             3507..>4476
                     /locus_tag="VII50_03515"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: protein motif:HMM:NF012307.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="reverse transcriptase family protein"
                     /protein_id="HEY5049943.1"
                     /translation="MALLAGPWDAPGMRRSVTSALGRDRQPRWVQRLVSQVLEVYPSP
                     PRDSPRELADVLQSLPAWLAGEQSSRPPHVTHWVTEPTAMGAMPWPVMALPHLAALCR
                     LLDVDAGELEWFADTRGLERQVGMPLRHYRWAMLPKRTGVRLVAAPKPRLKEIQRRLL
                     RHVLAPVPLHALAHGGVSGRSVRTALAPHAGAPVVIRADLEAFFSSIAGPRIYGVLRT
                     AGYPEAVAYAITGLCTTVVPRVVWDGVTVPDDFARHRRLGQLLAVPHLPQGAPTSPAL
                     ANLVAFSLDRRLAGLARAFDARYTRYVDDLTFSGGSRLRSGRSAFLA"
BASE COUNT          726 a         1417 c         1532 g          801 t
ORIGIN      
        1 ccgctccagg ccacggtgcg gtagttcggg ttctcgagcg tctcggtctc caggtcgaac
       61 gcctggggac ggggaccgat gtctgaaatg cccatggttt gaccctagcc ctgcccggac
      121 ccgatcggga tgcggagtgc gcgttatcgc ggcagagtcg gatggccgga tggtgccgca
      181 cgtggcgact ctgttcggtg ccagcttgct ccatgaccat ctagctcgga ggctgtcgcg
      241 ccggatcacc tctcgccttc atggccgcgc gcccggcggg caaccgtagg gattggtctg
      301 aatctgcttc gcgatgacaa caccatctag cagggctaca tatgccgagc cacccaggca
      361 atgggtgtcc aacggcacgg cgtcgagttg cagataccgg ccggtggtgt cgatgtgcac
      421 gtctaccgtg atccagccgc cggtgagttc gggtattgac gcgaatgcaa agacctgacc
      481 gttatgcaca cgctcgatgg ttaccgcggt ctcttggcag taagcgacga agtacacgtc
      541 gccgttcggg ctcattgcgc ttgcctccgg actgtccgga caagggcttt gtggtgcgct
      601 cggttgaacg acggtgctcg aagaaatcgg gtgctcggtg gcgtcgaacg tcaaggctcg
      661 aagctcgcgg gtgggcccgc cgaagtagta caactgccgg tcatccggtg accacccaac
      721 caatcgatag ccggtgaagc catcaggcag ttccggtccg gtgacggtcg acgggacgac
      781 gaggggtgcc gttgctcctg cgccttcgtt gaggacgaag atgttcgtac cgagcggggc
      841 cagcgttccg gtcggggccg ggatcgctga actcggaaga ccggtccggc cgacggtgat
      901 gacgtacgcg agaaggcgac cgtctggtga aatctgcggc gaaccaagtc cgacgccctc
      961 gtcgccgttg ttttgcggag ccgtgatgat cggcgtcgat ggaccgccgt gcacagacac
     1021 gcggtggatc gtgttgatcg ctgctgtggc cgtgttctgt gtctgtcgaa agtacacagt
     1081 gccggcggcc acgctaagcg tcggatcgta gtcggtcacg ccgctcgtgg gagtcacgtc
     1141 ggtgtcctca ccgctcgtga gcgtggtggc ggtggcctct ccaccttgat cccgacagat
     1201 tgcgatctgc ttcgacacgc acatgtactc gccgtcagca ggcagcgaga caccggttgc
     1261 gcttgaactc tcggacaggt acgaactcgc gggcgcggag gtcagccagg gtggaaacga
     1321 cccggtcttc gcaagcggcg gcggcacgga caaaggagcg gacgtcggcg agggcggtgg
     1381 attcgtggcg ctggtcgccg acccaccaga accacaggcg gccaccagga agaggacggc
     1441 gaacgcagtc gaaacgcggc cattcatcga tgctcagacg ccagctctgc gttcgcggtt
     1501 ccgcacccgt ggcactaccc gacagcgagg cattgcgcgg gaaggtcgaa ccacctgagg
     1561 tgctcgaagt cttcggacag agctcccgac gggcgatccg cttccgaggc gtcatcgcct
     1621 gcgctggcgg caaatagcga caccgcctcg gcgaggtagc tcgcgagctc cgactcggcc
     1681 gcgcgatcgt catgatgcgg gtagcccatc gcgccgtccg cctcgtagtt gacttggctc
     1741 agccgaagcg gaggttcgac cagtccgcgt cggtgacgcc acaacccggt ggccgggtcg
     1801 aagaggtagt cggtcaggag ccgccacccg tcgcgcgcga ccagccgaac ggcgtccaca
     1861 atgtaggtga aaaccggctc agagatgaag tagttgaagt tgacgcggac ccacccaggt
     1921 ttgattcctt cgcagccgcc ggcaatctcg cgctcgaact cgtgcgatcg atccaggtcg
     1981 attccgagca gccgatgccc gtacgggccg gcacaggaac acccgccgcg cgattggatt
     2041 ccgaaaaggt cgttgagcaa cgcgacgacg aagttgtggt gcaagtagcg cccgctcggc
     2101 gcgcgcacca cgaacgagac gatcgacagt cgttcggcgt cgagatcacc cagaatctcg
     2161 atcgccggct cggcccgcca cgcggtgact gcgcggcgca ggtagtgctc ctcgtgggcg
     2221 cggatcacgt cgatgcccac ggcctgcttc agctgaaaga cgaggccggc gcggatcgat
     2281 tcgacgattg ccggagtgcc gccctcctcg cgttgcgccg ggtcggtgag gtagcggtgc
     2341 tcggtggggt tcacataggc gacggtgccg ccgcccggga cgtcgggaac gcggttgtgc
     2401 agcagctcgc gtcgaaccac cagcacgcct ggcgtgctcg gaccgccgat gaacttgtgc
     2461 gggctgagga agatcgcgtc cttatgggcg agcggatcgg caccgccgta catgttgatg
     2521 tcgacatacg gtgcggccgc agcgaagtcc cagaaggaca gcgcgccgtg ccggtgcagg
     2581 agcgccgata tcgcgtaggt gtcgctgacg attccagtga cattgctcgc tgcggagaac
     2641 gacccgatct tcaccgaccg atcgttgtag cggacgagtt cgtcttccaa ggccgatgcg
     2701 tcgatgtgac cgtcggcgtc ctgcggaatg acgacgacgt cgacgatcga ttcgcgccac
     2761 ggcaactcgt tggagtgatg ttcgaacggc ccgatgaaca cgacgggccg ctgcgctggg
     2821 gggatcgcat cgctgaggtg gtaacggtcc tccagttccg cggggatacg caggccgagg
     2881 attccgatga gcttgtcgat tgcccctgtg cagcctgaac cggcgaagat cacgaccgtc
     2941 tcgtcgtcgc cacccactgc atcgcgaatg atccggcgcg cgtcttcgcg caggcgggtc
     3001 gtctgcaggc ccgtgccgct cgactcggtg tgcgtgttcg cgtatcgcgg caggacctcg
     3061 tcgcggatga agtcctcgag gaaactcaga gcacggccgg acgccgtgta gtcggcgtag
     3121 gtgacccggc gcggcccgta ggggcctggc atgacctggt cgtcgccgat gacggactcg
     3181 cggacacggc gcaacagtgc cgtttcgcgc agaaccggtt ctgcgtccgg gcgctgctca
     3241 tgcgggtcca tgccctgacc gtaccggtcg actaccggag tcgtccgcgt tagcgttcgg
     3301 gtcgtgaggc gggatgggag cgggctggtc gggttggccc gatcacttcg gggaaacccc
     3361 ggggtcatca cgcagagcag gtcatcgcat cgcacgccgg ctcgttcact tcgtgaacca
     3421 cgcggcgcgc acggcatgga atggccgccc ccatcccggt ctcacgtcgc atgacccacg
     3481 acccgactgc ggctcgtgga gtggccatgg cgcttcttgc cgggccgtgg gacgcgccgg
     3541 gcatgcgccg cagcgtcacc tcggcgcttg gccgggaccg gcagccgcgc tgggtgcagc
     3601 gactggtgtc tcaggtgctt gaggtctacc cgtcgccccc gcgtgacagc cctcgcgaac
     3661 tcgccgacgt ccttcagtca ttaccggcct ggctcgccgg ggagcagtca agtcggccgc
     3721 cacacgtaac gcattgggtg accgagccga ccgcgatggg cgccatgccg tggccggtca
     3781 tggctctgcc gcaccttgcg gcgctctgcc ggttgctcga tgtagacgcc ggcgagttgg
     3841 aatggttcgc ggatactcgc gggcttgagc gtcaggtggg catgccgctc cggcactacc
     3901 ggtgggcgat gctgccaaag cgcaccggtg tgcgattggt ggctgcacca aagccacgat
     3961 tgaaggagat tcagcgtcgc ctgttgcgcc acgtcctagc gccggtgccg ctgcacgcac
     4021 ttgcacatgg tggcgtttcg ggacgatccg tgcggaccgc gctcgcgccc cacgcaggcg
     4081 cgcccgtcgt gattcgcgcc gacctggaag cgttcttcag ttcgatcgct gggccgcgga
     4141 tatacggcgt ccttcgcact gcgggctacc ccgaggcggt ggcatacgcg atcacggggt
     4201 tgtgcacgac cgtcgttccg cgggtggtct gggacggcgt tacggttccc gacgatttcg
     4261 cgcgccatcg cagacttggt caattgctgg cggttccgca tttgccgcag ggtgcaccga
     4321 cgtcgcccgc gctcgcaaac ctcgtcgcgt tctcgctcga ccgtcgcctc gccggccttg
     4381 cccgcgcatt cgacgccagg tacacgcgct atgtcgatga cctgactttc agtgggggct
     4441 cgcgtttgcg ctccgggcgt agcgcatttc tcgcca
//
LOCUS       DATCBJ010000173         3072 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_130385, whole genome shotgun sequence.
ACCESSION   DATCBJ010000173 DATCBJ010000000
VERSION     DATCBJ010000173.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 3072)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3072)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3072
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_130385"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(184..1389)
                     /locus_tag="VII50_03520"
     CDS             complement(184..1389)
                     /locus_tag="VII50_03520"
                     /inference="COORDINATES: protein motif:HMM:NF042449.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase assembly
                     protein OpcA"
                     /protein_id="HEY5049944.1"
                     /translation="MNRDLHDTNSSAILTALTETRRSSGSAALGMVLTLVIDATESEH
                     YDALKAANSAAREHPCRIVTVIRRPGKSAPRLDAEIRVGNENLLGEVIILRLYGLLRE
                     QADSIVLPMLLPDAPVLVWWPGQGPAVPSLDPLGLLAQRRVTDAASSTRPTESLRERA
                     LDHSPGDTDFAWTRITPWRTLLAAALDQPFDDIIGGEVGAARNNASAELLAAWLEDRL
                     DIEVARKTTRGPGITDVRLLTRRGEITITRPDGRLASLVRTGQPDRAVPLPRRATDEL
                     LAEELRRLDPDDVYNETITCLFGTHPRAGRRTAAADAQAQELTRRPAPARKSAAARAS
                     DVADTPARGKTVRASKKAAANKAATKKALPRAKTTAEKAVLAKKAPAARRAPARKASP
                     AKKVARRGA"
     gene            complement(1386..2924)
                     /gene="zwf"
                     /locus_tag="VII50_03525"
     CDS             complement(1386..2924)
                     /gene="zwf"
                     /locus_tag="VII50_03525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HEY5049945.1"
                     /translation="MSPVGREAINPLRDPRDRRLPRVAGPCGIVLFGVTGDLARKKLM
                     PAIYDLANRGLLPPGFALVGFARRDWADEDFAQVVHDAVKQYARTPFREEVWTQLAEG
                     FRFVPGDFDDNAAFDALAQTINDLDRVRGTGGNHAFYLSIPPKLFPVVVAQLKRSGLS
                     AKNGDQWRRVVVEKPFGHDLKSARALSDTLSDVFPPDSVFRIDHYLGKETVQNILALR
                     FANTMFEPIWNRSYVDHVQITMAEDIGIGGRAGYYDGIGAARDVIQNHLLQLLALIAM
                     EEPVSFEAQSLRLEKTKILSAVRIPDDLAAYTARGQYASGWQGGQKVLGYLDEDGIPK
                     SSKTETFAAIKLEVDTRRWAGVPFYLRAGKRLGRRVTEVALEFQRAPHLPFAATATEE
                     LGQNALVIRIQPDEGVTMRFGSKVPGTAMEVRDVNMDFAYGESFTESSPEAYERLILD
                     VLLGDPPLFPRHEEVELSWEILDPIEDFWEKLGQPEQYRSGGWGPDSSIKMLARDGRV
                     WRRP"
BASE COUNT          478 a         1003 c         1049 g          542 t
ORIGIN      
        1 gctggagacg ccgccagttc ggcgagcacc gcggtgccca cgccaccgcc ggtcagcacc
       61 atgtgggcat gtccgcgcgc cgactgggcg tcgacgatgc gggtgacgag ccgggccgca
      121 actgcacgcg caagcagggt ggcgtcacga tggacgacga tctccgccgt gctcactgtg
      181 gactcacgct ccgcgccgag ccactttctt ggccggtgac gccttccttg ctggcgcacg
      241 tcttgcggcc ggcgccttct tcgcaagcac ggctttctcc gcagtggttt tcgcgcgagg
      301 caaggccttc ttcgtcgcgg ctttgttggc cgctgccttc ttgctggcgc ggaccgtctt
      361 gccgcgcgcc ggagtgtcgg ccacatcgga tgcgcgagcc gcggcggact tgcgggcggg
      421 tgctggtcgc ctcgtcagtt cttgagcttg cgcgtcggcg gcggctgttc ggcgtccggc
      481 ccgagggtgg gtgccgaaca ggcaggtgat cgtctcgttg tagacgtcgt cggggtcgag
      541 cctgcgtagc tcctcggcga gcagctcgtc ggtcgcgcgc ctcggcaacg gcaccgcacg
      601 gtcgggctgg ccggtgcgaa ctaggctggc gagccggccg tcgggacggg tgatcgtgat
      661 ctcaccacgc cgtgtcagca gccggacatc ggtgatcccg gggccccggg tggtcttgcg
      721 cgcgacttcg atgtcgaggc ggtcttcgag ccacgccgcc agcagttctg cgctggcgtt
      781 gttgcgcgcg gcgccgacct cgccgccaat gatgtcgtcg aacggctggt cgagggccgc
      841 cgcgagcagg gtgcgccatg gagtgatccg cgtccacgcg aaatcggtgt cgccggggga
      901 gtgatccagc gcgcgctccc gaagcgattc ggtcggcctc gtggaggatg cggcgtcggt
      961 gacccggcgc tgggcgagca ggccaagcgg gtcgagtgac ggaactgccg gaccctggcc
     1021 cggccaccac accagcaccg gagcgtcggg aagcagcatg gggagcacga tcgagtcagc
     1081 ctgctcccgc aagagcccgt acagccgcag gatgatgacc tcgccgagca agttctcgtt
     1141 gccgacccgg atttccgcat caagccgcgg tgccgatttg cctggccgcc gaatcacggt
     1201 gacgatccgg cacgggtgtt cgcgggcggc cgagttcgcc gctttgagtg cgtcgtagtg
     1261 ctcgctctcg gtcgcgtcga tgacgagcgt caacaccatg ccgagcgcgg cgctgccaga
     1321 gctccgtcgc gtctcggtca gcgcggtcag gatcgccgac gaattcgtgt cgtggagatc
     1381 gcggttcatg gccgcctcca aacccgtccg tcgcgcgcga gcatcttgat cgacgaatcg
     1441 gggccccagc cgccggatcg gtactgctcg ggttggccca gcttctccca gaaatcctcg
     1501 atgggatcga gaatttccca cgatagttca acttcttcgt gccgagggaa aagcggcgga
     1561 tcaccgagca ggacatcgag aatcaaccgc tcgtaggcct cgggactcga ctcggtgaag
     1621 gactcgccgt acgcgaagtc catgttgacg tcgcgtactt ccatcgcggt gccgggaacc
     1681 ttcgagccga accgcatcgt gacgccctcg tcgggctgga tgcggatgac gagcgcgttc
     1741 tgcccgagtt cttcggttgc ggtcgccgcg aatggaaggt gcggggcccg ttggaactcc
     1801 agcgcaacct ccgtcactcg gcgtcccagc cgcttgccgg cccgcaggta gaacgggaca
     1861 ccggcccagc ggcgggtgtc gacctcgagc ttgatggcgg cgaacgtctc ggtcttcgac
     1921 gacttgggga tgccgtcctc gtcgaggtag ccgagcacct tctggccgcc ttgccacccg
     1981 ctcgcgtatt gaccccgcgc ggtgtatgcc gcgaggtcgt cggggattcg aaccgcagac
     2041 aggatctttg tcttttccag gcgaagcgac tgcgcctcga agctaactgg ctcctccatc
     2101 gcgatgagag caagcaactg cagcaagtgg ttttggatga catcgcgcgc ggcgccgatg
     2161 ccgtcgtagt agcccgcgcg tccgccgatg ccgatgtctt cggccatcgt gatctgcacg
     2221 tggtcgacgt aggagcgatt ccagatcggc tcgaacatgg tgttggcaaa gcgcagcgcg
     2281 aggatgttct gcactgtctc cttgccgaga taatggtcga tgcgaaagac cgagtcgggt
     2341 gggaagacgt cgctaagcgt gtcgctcagt gctcgcgcgc tcttgaggtc gtgaccaaac
     2401 ggcttctcga caacgacccg ccgccactgg tcaccgttct tggccgacag tcccgatcgc
     2461 ttcagctgcg cgacgaccac cggaaagagc ttcggcggta tcgacaggta gaacgcgtgg
     2521 ttgcccccgg tgccccgcac gcggtcgagg tcattgatcg tctgcgcgag cgcgtcgaat
     2581 gcggcgttgt cgtcgaagtc cccgggcacg aaacggaatc cctcggccag ttgggtccac
     2641 acctcctcgc gaaagggtgt gcgggcgtac tgcttgacgg cgtcgtggac gacctgtgcg
     2701 aaatcctcgt cggcccagtc acggcgggcg aatccaacga gcgcgaaccc gggtggcagc
     2761 aggccacggt tggcgaggtc atagatggcc ggcatcagct tcttacgtgc caagtcgccg
     2821 gtgacgccga acaacacgat tccgcacggc ccggccaccc tcggcagccg tcgatcgcgc
     2881 gggtcacgga gcgggttgat cgcctcgcga ccgaccgggc tcatcgagcc aaaccggcgg
     2941 cgttgagcag ctcctgaagg ccgaccgtgc gatcgagcag atgcaaccgg atcgccggcc
     3001 gccctcgttg ctgcaacgcg cgcaagtcac ccaacgcctg cgccatttgt aaccgggcga
     3061 acccgaatgg cc
//
LOCUS       DATCBJ010000174         2321 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_388448, whole genome shotgun sequence.
ACCESSION   DATCBJ010000174 DATCBJ010000000
VERSION     DATCBJ010000174.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2321)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2321)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2321
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_388448"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1018
                     /gene="nuoN"
                     /locus_tag="VII50_03530"
     CDS             <1..1018
                     /gene="nuoN"
                     /locus_tag="VII50_03530"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoN"
                     /protein_id="HEY5049946.1"
                     /translation="RRRLLSQEAAVKYFLLGAFSSAFFVYGIAMVYGFADTVQFSGIA
                     DALTSSSRGDSLILLGVALVGIALLFKLSGAPFHWWTPDVYQGAPTSITAFMAAGTKI
                     AAFGALMRVFFVAFGGLSWDWRPVMWVIAILTMSVGAIVGVTQTDVKRLLAYSSIAHA
                     GFILTAIAALAPGSARDSESGVMFYLVAYGFMTIGSFAVVMLVRDGDGEANHLSRWVG
                     LGRRSPLVAGIFAFFLLALAGLPGTSGLWAKVAVFKAAGEGGAWPLVVVGVVASAVTA
                     YYYVRIIVLMFFQEPLPDGPTVVLPQFFTSTAIGLGLAVTVVLGVVPQPLLNLAGKAG
                     LFLK"
     gene            1031..2053
                     /locus_tag="VII50_03535"
     CDS             1031..2053
                     /locus_tag="VII50_03535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HEY5049947.1"
                     /translation="MTGQEVFGVSSEGPFGLVVPDPALEAEIRVGIDAVEALLRDAVK
                     SDYPFVTETSRHLVVAGGKRFRPLLVLLAAQFGDPAAPGVLPAAVVVELTHLATLYHD
                     DVMDEALVRRGTISANARWSNTIAILTGDFLFSRASDLLADLGPEAVRIQARTFERLV
                     QGQIRETTGPHDGADPLEYYLQVVADKTGSLIATSARFGAMFAGAPESVVEVMIRYGE
                     RIGVAFQLSDDLLDVTSDATGFGKTPGTDLREGVVTLPVLHARRSIDPADARLLELLD
                     GDLTDAGRHRETLGLLRSHPAIEATRADLDRWASDARSTLVPLPDGPAKRALEALCDF
                     VVARTR"
BASE COUNT          324 a          748 c          780 g          469 t
ORIGIN      
        1 ccgacgccgc ctgctttcgc aggaggccgc agtcaagtac ttcttgctcg gcgcattctc
       61 gtcggcgttc ttcgtctacg ggatagctat ggtctacggc ttcgccgaca cggttcagtt
      121 ctccggcatc gccgacgcgc tcacctcgtc gagcaggggc gactcgctga ttctgctcgg
      181 tgtcgcactg gtcggaattg ccttgctgtt caagctttcc ggtgcgccgt tccactggtg
      241 gacccctgac gtctatcagg gtgcgccgac ctcgatcacc gcgttcatgg cagctggcac
      301 gaagatcgcc gcgttcggtg cgttgatgcg cgtgttcttc gtcgccttcg gcgggcttag
      361 ctgggactgg cgcccggtca tgtgggtgat agcgatcctc acgatgtccg tcggcgcgat
      421 cgtcggcgtc acccagaccg atgtgaagcg gttgctcgcg tactcgtcca tcgcccacgc
      481 cggtttcatc ctgaccgcga tagcggcact cgcccctggt agcgcacgcg acagcgagtc
      541 aggggtgatg ttctacctcg tcgcgtacgg cttcatgacg atcggatcgt ttgccgtggt
      601 gatgctcgtg cgcgacggcg acggtgaggc gaaccatctg tcgcgatggg ttggccttgg
      661 ccgccggtcg ccattggttg cgggcatctt cgcgttcttc ttgttggccc tggccgggct
      721 gccaggtacg agcggcctgt gggcgaaggt ggcggtgttc aaggccgcgg gcgaaggcgg
      781 cgcttggccg ctggtggtgg ttggggtggt cgcgagtgcc gtcactgcct actactacgt
      841 gcgcatcatc gtgctgatgt tcttccagga gccgcttcct gacggtccga ccgtcgtcct
      901 gccgcagttc ttcacctcga ctgccatcgg gctcggcctt gcggttaccg tggtgctagg
      961 cgtcgttccg cagccactgc tcaatctcgc cggcaaagct gggctgttcc tcaagtagca
     1021 cgaagtcgcc atgacagggc aggaggtctt cggcgtgagc agcgaaggcc cgttcgggct
     1081 cgtcgtcccc gatccggctc tggaggcgga gatccgggtc ggcatcgacg ccgtcgaggc
     1141 gttgttgcgc gatgccgtca agagcgacta ccccttcgtc accgagacat cgcggcatct
     1201 cgtcgtcgcg ggcggcaagc ggtttaggcc gctgctggtc ttgctcgcgg cgcagttcgg
     1261 cgatccagcc gcgcccggtg tgctgccggc tgcagtggtc gttgaactga cccacctcgc
     1321 gacgctttac cacgacgacg tgatggacga agccctcgtc cggcggggca cgatcagcgc
     1381 gaacgccagg tggtcgaaca cgatcgcgat cctcaccggc gacttcttgt tctcgcgagc
     1441 ctccgacctc ctcgccgatc tcggtcctga ggctgtgcgg atccaggccc ggacgttcga
     1501 gcgactcgtc cagggccaga tccgcgagac gactggcccg cacgacggcg ccgacccgct
     1561 tgagtactac ctgcaggtcg tcgccgacaa gaccggctcg ctgatcgcga cgtccgcgcg
     1621 ttttggtgcg atgttcgctg gggcgcccga gtcagtcgtc gaggtgatga tccggtacgg
     1681 cgagcggatc ggtgttgcgt tccagctgtc cgatgacttg ctcgacgtga cgagtgacgc
     1741 gaccggattc ggcaagacgc caggcaccga cttgcgtgaa ggtgtcgtca ccttgcccgt
     1801 gttgcatgcg cggcggtcga tcgatccggc agacgcccgg ctcctcgaac tgctcgatgg
     1861 tgatctcacg gatgcgggtc ggcaccgcga gacgctgggc ctgctccgtt cgcacccggc
     1921 aatcgaggcc acccgcgcgg acctcgacag atgggcgagc gacgcgcggt cgacgctggt
     1981 gccgcttccg gacggccccg cgaagcgtgc cctcgaggca ctgtgcgatt tcgtggttgc
     2041 ccgtacccgc taggtcagtg gcggcacggt ttccgtcgcc ggccagatga gggctcgaaa
     2101 tcttcctggc gccccgggga tgacaccgct tacaaaccgg ctatcgttcc gggtgcacaa
     2161 cccccgtcga ggagaggatc acccgggtgg tcactgacgg agctgcgctg ctcggcatca
     2221 cgatccgcga tgtggcccgt gaggcaggtg tgtccaccgc gacggtatcc cgagcactgc
     2281 gagggttgcc gaacgtcgac ccggtcacgc gtgaacatgt g
//
LOCUS       DATCBJ010000175         2120 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_68900, whole genome shotgun sequence.
ACCESSION   DATCBJ010000175 DATCBJ010000000
VERSION     DATCBJ010000175.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2120)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2120)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2120
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_68900"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..275
                     /locus_tag="VII50_03540"
     CDS             <1..275
                     /locus_tag="VII50_03540"
                     /inference="COORDINATES: protein motif:HMM:NF013843.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HEY5049948.1"
                     /translation="PDLVVYLQARTDVLLDRIRKRGRPEEKPIRAEYVEEVAKAYAEF
                     FFGYSEGPLLIVNASEIDFVGNLEHRRELVAVIENTHVGTNHWSRG"
     gene            complement(291..1310)
                     /locus_tag="VII50_03545"
     CDS             complement(291..1310)
                     /locus_tag="VII50_03545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5049949.1"
                     /translation="MACAPPADPDQPLQFLSQTGCMDSTTPTMLASVVVPYVVQSPLW
                     SDGADKSRGMRLPPGGKIHVKHCTGPSTECLDAADDGKWVFPVGTVMVKSFIFNGKLI
                     ETRLFTNHDAGDWVGYSYAWNTAQTEATIVPDQRTEVMFDTGQGTPVDWHYPSRLDCM
                     TCHTPTGGDTLGPSTPQMNRVNAGATQNQLDLLQAVNLFDAPIPTPYKAALPTPYTSQ
                     VGNQTAGATTTQLARSYLAANCAFCHRPDDPNFPSMDFRYDVAFADMGICGATPLKGD
                     EGVNNSLLLAPAQPADSVVWLRMDATVDNGRMPKLASYVIDQNAVTLVGSWITSITSC
                     PAPTM"
     gene            1632..>2120
                     /locus_tag="VII50_03550"
     CDS             1632..>2120
                     /locus_tag="VII50_03550"
                     /inference="COORDINATES: protein motif:HMM:NF013199.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5049950.1"
                     /translation="MSFHRHLLVGIALWGAACSSKSSGSAGSQSVLERNNHPSRDGSF
                     IQPALTKAKAALMTADTAFVPNLNASTVANGNNVWASPLYLQNGPGGKGVFFAVTTGN
                     DVYALDETTGATIWTHNIGSSPTANGVSCGSIHPLGIISTPVIDAASRTIYVAGAIGT
                     TSI"
BASE COUNT          334 a          708 c          729 g          349 t
ORIGIN      
        1 cgcccgacct ggtcgtctac ctgcaggcgc gcaccgacgt gctgctcgat cgcatccgaa
       61 agcgcggtcg cccggaagaa aaacccatcc gcgcagagta cgtcgaagag gtggccaagg
      121 cctacgccga gttcttcttc ggctacagcg agggcccgct gctgatcgtc aacgcctccg
      181 agatcgactt cgtcggcaac ctcgagcacc gccgcgagct ggtcgccgtg atcgaaaata
      241 cccacgtcgg caccaaccac tggagccggg gctgagcgcg gtcggccggt ctacatggtc
      301 ggcgcggggc agctggtgat cgaggtgatc cagctaccga ccagcgtgac cgcgttctga
      361 tcgatcacgt agctggcgag cttgggcatc cggccgttgt cgacggtggc gtccatgcgc
      421 agccagacca ccgagtccgc gggctgcgcc ggggccagca ggagtgagtt gttgacgccc
      481 tcgtccccct tcaggggcgt ggcgccgcag atgcccatgt ccgcgaaggc gacgtcgtat
      541 cggaagtcca tgctcgggaa gttgggatcg tcggggcggt ggcaaaaggc gcagttggcg
      601 gccaggtatg agcgcgcgag ctgcgtcgtc gtcgcgccgg ccgtctggtt ccccacctgc
      661 gacgtgtagg gcgtcggcag cgccgccttg tacggggtgg ggatcggggc gtcgaagagg
      721 ttcaccgcct gcagcaggtc gagctgattt tgcgtcgcgc cggcgttgac ccggttcatc
      781 tgcggcgtcg acgggcccag cgtgtcgccg ccggtcggcg tgtggcaggt catgcagtcg
      841 agccgcgacg ggtagtgcca gtcgaccggc gttccctggc cggtgtcgaa catgacctcg
      901 gtccgttgat cgggaacgat ggtggcctcg gtctgggcgg tgttccaggc gtagctgtaa
      961 ccgacccagt cgccggcgtc gtgattcgtg aagaggcggg tctcgatcag cttgccgttg
     1021 aaaatgaagc tcttgaccat cacggtcccg accgggaaga cccacttgcc gtcgtcggcc
     1081 gcgtccagac actcggtcga tggaccggtg cagtgcttga cgtggatctt cccgccgggc
     1141 ggcaagcgca tgccgcgcga cttgtcggcg ccgtcggacc acagcgggct ctgcaccacg
     1201 taggggacga cgaccgacgc cagcatcgtg ggcgtcgtgg aatccatgca gccggtctga
     1261 ctgaggaact gcagcggctg atccgggtcg gccggcggcg cgcaggccac ggatgggctc
     1321 gcaccgtcgg tcccggcgtc ggctcgtcgg tcggatgggg cgtcccgcgc ggcgtccgac
     1381 gaagtgtccg atgaaccatc gtgccgcgcg tccgtggcac ccccgtcgtg tccgccgacg
     1441 ttggtcgacc cgtcgcccgg cgccgcggcg tctcccttgc cacccgattt gctgccgcag
     1501 ccggccgcgc cgattgtcag gagcagaatt gaagttggcg taagatagcg gcccacgccg
     1561 gccaggcgtt tcgcccccgt cgattccacc gacgaaaagt atcacgcggg gcctctcacg
     1621 gagggagatc catgtcgttt caccgccatt tgttggttgg aatcgccctg tggggcgccg
     1681 cgtgctcgtc gaagagcagc ggcagcgcgg gctcgcagtc ggtgttggag cggaacaacc
     1741 accccagccg cgacggcagc ttcatccagc ccgcgctgac caaggccaag gcggccctga
     1801 tgaccgccga caccgcgttc gtccccaact tgaacgccag cacggtcgcc aacgggaaca
     1861 atgtctgggc ctcgccgctc tatctgcaga acggccccgg cggcaagggc gtgttcttcg
     1921 cggtgaccac cgggaacgac gtctacgcat tggacgagac caccggcgcc acgatctgga
     1981 cgcacaacat cggcagctcg ccgaccgcca acggcgtgag ctgcggcagc atccatccgc
     2041 tcgggatcat cagcacgccg gtcatcgacg ccgcgtcgcg cacgatctac gtggccggcg
     2101 ccatcggcac gacctcgatc
//
LOCUS       DATCBJ010000176         2679 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_499514, whole genome shotgun sequence.
ACCESSION   DATCBJ010000176 DATCBJ010000000
VERSION     DATCBJ010000176.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2679)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2679)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2679
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_499514"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(157..780)
                     /locus_tag="VII50_03555"
     CDS             complement(157..780)
                     /locus_tag="VII50_03555"
                     /inference="COORDINATES: protein motif:HMM:NF013641.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HEY5049951.1"
                     /translation="MSSITLIGTGNMARTIGTLAVAGGNSVEVIGRDQSKADDLADAL
                     GGGATTGKWGAVPAGDIVIAALLYDAVVPVVAEFGAALAGKVIVDISNPFNATFDGLA
                     HREETSIAQEVAKVAPADASVVKGFNTIFQNVLEKGRPNVFIAGDNAQAKAGVAAFIE
                     SLGLRPLDVGSLKMAHWLEGMGVVTVGLAGNGVGHWDFSLGVNELAG"
     gene            complement(1204..2124)
                     /locus_tag="VII50_03560"
     CDS             complement(1204..2124)
                     /locus_tag="VII50_03560"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEY5049952.1"
                     /translation="MVASPLHDTTTNPPAGGPVVASAAVAHLARYRGLSRSHTESDLR
                     IFFRWCDNRSLDVLAVQRVDLELYLRWMQDVRGFKASTVSRRLSVVAGFYRTCVIDHV
                     LMQSPAEHVRRPNVPPESPTLGLNHLQFEALLAAARTSTNQFDFALICLLGLLGLRIF
                     EATGANIDDLGEEHGHRVLKVRGKGNKVVLVPLPPAVARAVERARGDRDTGPLLLNRR
                     GTRMDRHSATRRLRRLAADAGVRLPRMHPHMLRHTFVTTMLDAGVDLRDVQIAARHAD
                     PRTTMRYDRARKNLDRHPNYILAAYMASGT"
     gene            2222..>2679
                     /locus_tag="VII50_03565"
     CDS             2222..>2679
                     /locus_tag="VII50_03565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5049953.1"
                     /translation="MDMTPDRALLAAMYGGDGAAVLAVLTGDGRGLLRSMPQLAGDGL
                     VCALAQQLPLAQQLVDEVAGVLRQRDWDGDAELADQIDPAVADPDQVPLRPLHIDLEQ
                     LAAILEGDPRDGGGRIDLTTGEVWPQAAFDYGGTTEIEDEDEEEDEEALR"
BASE COUNT          419 a          852 c          935 g          473 t
ORIGIN      
        1 ggagtgggca tccctcgacg atgtcatggg gatgcaacca tcctcaactg ccggcgatga
       61 cgcacgttcg gacctgccga tttgcgtgcc tgcgttttcc ggcttccgtc catccacgag
      121 gacggcgcag ggcagcgagt cggtgatgcg ccggggtcag ccggcgagtt cgttgacgcc
      181 gagcgagaag tcccagtggc cgaccccgtt gccggccagg cccaccgtga ccacacccat
      241 tccttccaac cagtgcgcca ttttcaggct gccgacgtcc agtgggcgta gcccgaggct
      301 ctcgatgaat gccgccacgc ccgccttggc ctgcgcattg tcgccggcga tgaagacgtt
      361 gggccggccc ttctccagga cgttttggaa gatggtattg aaacccttca cgacgctggc
      421 gtcggccggg gccaccttgg cgacttcctg cgcgatcgag gtctcctcgc ggtgggccag
      481 tccgtcgaag gtggcgttga aggggttgct gatgtcgacg atgactttgc ccgcgagggc
      541 cgctccgaac tcggcgacga ccggaacgac ggcgtcgtac aacagggccg cgatgacgat
      601 gtccccggcc gggacggcgc cccacttgcc cgtcgtcgcg ccgccgccca gagcgtcggc
      661 gaggtcatcg gccttggact ggtcgcgtcc tatgacctcg acgctgttgc cgcccgccac
      721 cgcgagcgtg ccgatggtac gggccatgtt ccccgtaccg ataagggtga tgctgctcat
      781 gaggtgtgct ctcttcctgt actcgaggtg tcgctctgtc gcgttcgtgc cgggtcaggc
      841 gcagggctcg gccgcgacgt ttctgcgcta agtgccgtgc ctttcccggc acctgcggct
      901 cgtcttccgc cggtagatgt ggatgcgtga acctgccgcc attccaagag agggtcaggt
      961 cacggacacg ggacggggcg tccggtgatc gctactttcc ttggtgccca gttcccaggg
     1021 ccgcatgccg atgagacgtg aactcctgac atcgccgccg gcggactgcc gatgaaacct
     1081 ccggagcaat cgctagacgc acatccgctc ttgccgcgat gacgcgtgtg ggctttgccg
     1141 acttggttcg ctcgggtgac tcgaactgac tccgtatgtt gcgcgcgccg gtgcggtgat
     1201 tggtcaggtg ccggatgcca tgtaggcggc gaggatgtag ttggggtggc ggtcgaggtt
     1261 tttgcgtgcg cggtcgtacc gcatggtggt gcgcgggtct gcgtgtcggg cggcgatctg
     1321 cacgtcgcgc agatcgacac cggcgtcgag catggtggtg acgaaggtgt gccgaagcat
     1381 gtggggatgc atccggggca gtcgcactcc ggcgtcggcg gcgaggcggc gtagacggcg
     1441 ggttgctgag tgccggtcca ttcgggtgcc gcgtcggtta agcaggagcg ggccggtgtc
     1501 acggtcgccg cgggcccgct cgaccgcgcg ggcgaccgcc ggcggcaagg gcacgaggac
     1561 gactttgttg cctttgccgc ggaccttcag cactcggtgg ccgtgttctt caccgaggtc
     1621 gtcaatgttc gcaccggtgg cttcgaagat gcgcaggccg agcaacccga gcaggcagat
     1681 caacgcgaaa tcgaactggt tggtcgacgt gcgcgccgcg gccagcagcg cctcgaactg
     1741 caggtgattc agccccaagg taggtgactc cggtggcacg ttggggcgac gcacatgctc
     1801 ggccggagac tgcatgagca cgtggtcgat cacgcaggtg cggtagaagc cggcgaccac
     1861 cgataaccga cgcgacacgg tggaggcctt gaacccgcgc acgtcctgca tccaccgcag
     1921 gtacaactcc agatcgacgc gctgcacggc gagcacatcg aggctgcggt tgtcgcacca
     1981 gcggaagaag atccgcaagt ccgattcggt gtgggagcgg gacaagcccc ggtaacgcgc
     2041 cagatgtgcg acggcggccg atgccaccac cggcccaccc gctggcgggt tcgtcgtcgt
     2101 gtcgtgcagc gggctcgcga ccatccctca ccgtgcagtt gctcctgccg gctccgctag
     2161 ccgccgtccg ggtgacaggg actgctcgac ccggaccagg gttgggacgc aaaatatctc
     2221 cgtggacatg accccggacc gcgcgctgct cgcggcgatg tatggcggcg acggtgccgc
     2281 ggttctcgct gtgttgaccg gcgacggccg gggcttactg cggtcgatgc cgcaactggc
     2341 cggtgacggg ctggtctgcg ctctcgccca gcaactaccc ctcgcccagc aactcgtcga
     2401 cgaagtcgcc ggcgtcttgc ggcaacggga ctgggacggc gacgcggagc tcgccgatca
     2461 gatcgacccg gccgttgcgg atccagacca agtgccgctg cggccgctgc acatcgatct
     2521 cgagcaactt gccgcgatcc tggagggtga cccgcgcgac ggcggtggcc gcatcgacct
     2581 gaccaccggt gaagtgtggc cacaagctgc cttcgactac ggcggcacaa ccgagatcga
     2641 agatgaagac gaggaagagg atgaggaagc tttgcgttg
//
LOCUS       DATCBJ010000177         2224 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_499453, whole genome shotgun sequence.
ACCESSION   DATCBJ010000177 DATCBJ010000000
VERSION     DATCBJ010000177.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2224)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2224)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2224
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_499453"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(63..689)
                     /gene="ruvA"
                     /locus_tag="VII50_03570"
     CDS             complement(63..689)
                     /gene="ruvA"
                     /locus_tag="VII50_03570"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HEY5049954.1"
                     /translation="MIAFVAGRVASIAPDSVVVEVGGFGLQVYCAPTAISRVRVGESA
                     RFATSLVVREDSLTLYGFADDDERMVFELLQTATGVGPRLAQAMLGVHPPSALRRAVA
                     TDDFATLSLVPGIGRKTAQRIVLELKDKLAGATLTEDVVVLPGGSSASWRESVHGALL
                     GLGYSVREADDAVQAVAGDADAMVVAGDAPDVGVLLRQALRSLGGARV"
     gene            complement(686..1255)
                     /locus_tag="VII50_03575"
     CDS             complement(686..1255)
                     /locus_tag="VII50_03575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004520 - DNA endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HEY5049955.1"
                     /translation="MRSIRVLGVDPGLTRCGIGVVDSAGGGRVTLVEVDVLRTPSDAE
                     IGARLLALEVGFEAWIARHRPDVIAVERVFSQHNVRTVMGTAQAAAIAITCAARRGIP
                     VALRTPSEVKAAVTGQGRAGKEQVAFMVRKLLATPGLVAPADATDALALAICEVWRGA
                     ASAKVSIALEAAERRTTMNGAAIRTGSSR"
     gene            1515..>2224
                     /locus_tag="VII50_03580"
     CDS             1515..>2224
                     /locus_tag="VII50_03580"
                     /inference="COORDINATES: protein motif:HMM:NF024734.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin domain-containing protein"
                     /protein_id="HEY5049956.1"
                     /translation="MFQVAELPKNASAPPLSLARPFTYADAINAGYTRGLIRYLVSTG
                     RWLVLRRGVYCDIALSERADPVWLHATAASLVMTAGSAISHETAGTLCELPVGQRHAG
                     SPTTARQPPTIYLTHPPASRHARRGHPGVIERVAALPPSHLAYFQGLLITGAARTVVD
                     LARFRPYAEGVAIADAALHLEITTNAQLVAVRDACRSWPGINRASKVIEFADGAAESP
                     LESRSRVAFALGGLPTPTL"
BASE COUNT          372 a          828 c          668 g          356 t
ORIGIN      
        1 gacttcgatg ccgtcgacga tctcgatgcc atcggcgcgc acatcggcag gtgattgttc
       61 ggtcatacgc gtgccccgcc cagtgatcgc agcgcctggc gcaacagcac cccgacatca
      121 ggcgcgtccc cggccaccac catggcgtcg gcatcgccgg cgaccgcctg caccgcgtcg
      181 tccgcctcgc gaacggaata gccaagaccc agcagcgcgc cgtgcacgct ctcgcgccaa
      241 ctcgcgctgc tgccgcccgg caggacgacg acgtcctcgg tcagcgtcgc accggcgagc
      301 ttgtccttga gttcgaggac gatgcgttgc gcggtcttgc ggccgatgcc gggcaccagt
      361 gacagggtcg cgaagtcatc ggtggcaacc gctcgtcgca gcgcggacgg cggatggacg
      421 ccgagcatgg cttgggccaa ccgtggtccg acaccggtcg ccgtctgcag cagttcgaag
      481 accattcgct cgtcatcgtc ggcgaatccg tagagcgtca acgagtcttc gcgcacgacc
      541 agcgacgtcg cgaaccgcgc ggattcaccg actcgtaccc gcgagatcgc cgtcggcgcg
      601 cagtagacct gaagcccaaa accaccgacc tcgacgacga cggagtcggg ggcaatcgac
      661 gcgactcgac ctgcgacgaa cgcgatcatc ggctactccc agtacgtatc gctgccccat
      721 tcatcgtggt gcgccgctct gcagcctcga gcgcaatgct caccttggca cttgcggcgc
      781 cgcgccacac ctcgcaaatc gccagcgcaa gggcgtcggt cgcgtccgcc ggggcaacca
      841 agccgggagt cgccagcaac ttgcggacca tgaacgccac ctgctccttg ccggcacggc
      901 cctgcccggt gacagcggct ttgacctcac tcggcgttcg caaggcgacc ggaatgcccc
      961 ggcgcgcggc gcaggtgatc gcgattgcgg cggcctgcgc cgtgcccatc acggtgcgca
     1021 cgttgtgctg gctgaagacg cgttcgaccg cgatgacgtc gggccggtga cgcgcgatcc
     1081 acgcctcgaa accgacctcg agggcgagta gccgcgcacc gatctcggca tcggaaggcg
     1141 tccgtaacac gtcgacctcg accaaggtca cccggccgcc gcctgcgctg tcgacgacgc
     1201 cgatcccaca ccgggtcagc cctgggtcga cgccgagcac ccttatcgaa cgcatgttcg
     1261 gaagcgtagc ggcgcccgcg ccccgttcgg cgcagcgacg caccggtgcc ttcccgttcc
     1321 cgggtttcgc cacttcgcac cctccccttc cgcagcgcac cggtgtgggc ctgccctttc
     1381 ccaacttcac atccggtgtg gtcgcgattt ggtcgctcaa aggcggaatt cacaaccaca
     1441 ccggatgtga agtgctgtgc acagaccaaa ccgatcgcgc ggtacgcgca cctcgcgccc
     1501 tccatcgtcg tgtcatgttc caggttgccg agttgccgaa gaacgcttcc gcgcccccgc
     1561 tgtcgcttgc gcgtccgttc acgtatgccg acgcgatcaa cgccggctac acccgcgggc
     1621 tgatcagata tctcgtcagt actggccgct ggctcgtact ccgacgcggc gtctactgcg
     1681 acatcgcact ctcggagcgt gccgacccag tgtggctcca cgcgaccgcc gcatcactcg
     1741 tcatgaccgc cggatcggca ataagtcacg aaaccgcagg cacgttgtgc gaattgccag
     1801 tcggacagcg gcacgcgggc tcaccaacga ccgcgcgaca acctccgacg atctacctca
     1861 cccacccgcc cgcctcacga catgcacgac gcgggcatcc gggggttatc gagcgcgtgg
     1921 ctgcgctacc cccgtcacat ctcgcgtact ttcagggcct cctcatcacc ggcgcggcac
     1981 gcacagtcgt ggacctcgca cggttccgcc cgtacgccga aggcgtcgct atcgccgatg
     2041 ctgcgctcca cctggaaata acgacgaacg ctcagctcgt cgccgtccgg gatgcatgtc
     2101 gctcctggcc cggcatcaac cgtgcctcca aggtgatcga gttcgccgat ggggctgccg
     2161 agagcccgct cgagtcacgc tccagagtcg ccttcgcact tggcggactg ccgacgccca
     2221 ctct
//
LOCUS       DATCBJ010000178         3680 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_351777, whole genome shotgun sequence.
ACCESSION   DATCBJ010000178 DATCBJ010000000
VERSION     DATCBJ010000178.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 3680)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3680)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3680
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_351777"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(23..1624)
                     /locus_tag="VII50_03585"
     CDS             complement(23..1624)
                     /locus_tag="VII50_03585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009084860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEY5049957.1"
                     /translation="MSESTESRPTSSPGVAVLNADEKRLHELGYAQELHRGMGWFSNF
                     AVSFTIISILTGGITTYYLGMYAGGPRVIVWGWLFVGLMVMLVGAGMAEVCSSFPTAG
                     GLYYWAAKLAPEGKAPVWSWFTGWFNLLGQVAVTASIDFGMATFLGFFLNLTTGFNAT
                     PKSLFVIYTIVLVVHGILNTMGVNVIARLNDISVWWHLTGVVVIVGALFILPDAHKHQ
                     SLSNVFTKYYDGLGWHFGGHAIWVVLVGLMMAQYTLTGYDASAHMTEETRDAATSGPR
                     GIMTSIWVSVVAGLVLMIGLTYAIPGAIGGATYSAAAGAGINAPGVLWVASIGRHAAE
                     VLILVCLVAQFFCGMASVTANSRMIYAFSRDGAIPGSRFWHKVNKRTRTPTNSIWFAV
                     VGAFILGVPSLYTHKGVAVAFFAIVSVAVVGLYISYVIPVLLRRLRGSDFTPGPWQLG
                     KWSPLIGWLAVGWVALICFPLMLPQFSPTGPYSWNYAPAAVAAVIGFAGIYWLVSARK
                     WFTGPKVQGSAEELAAIEAELSSLG"
     gene            complement(1764..2492)
                     /locus_tag="VII50_03590"
     CDS             complement(1764..2492)
                     /locus_tag="VII50_03590"
                     /inference="COORDINATES: protein motif:HMM:NF019342.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HEY5049958.1"
                     /translation="MGRPIIGLTTYRENARWGVWDRLADIQSTLYTDAVAASGGAVVL
                     VPPADSGVGDVLRALDGLLISGGADVDPAEYDAEAHPAAGPFRADRDNAEFALVRAAV
                     EQGVPVLGICRGLQVLNVALGGDLIQHLPEIEGVLPHAEGRGVFSTRAVTLDSDSWIG
                     RTLGAGVPTACHHHQAVDRVAPGLRIVGRADDGTPEALEAADGRPVFGVQWHPEQLDD
                     RRLFEAFVNLATERALSRSAPTRA"
     gene            complement(2557..>3680)
                     /locus_tag="VII50_03595"
     CDS             complement(2557..>3680)
                     /locus_tag="VII50_03595"
                     /inference="COORDINATES: protein motif:HMM:NF013601.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEY5049959.1"
                     /translation="ADLRESPDVRREVNLTWMPMTPQSLLEGLYAEPHRLAAAAPHLD
                     DVQRALLRRDRGAPWTVSDVPLLDEAAELLGDDDTGGYELKRQAAQQRQSEVEYAKGA
                     LQISGDAGGLVSAEALAGRFADEGPILSAAERAAVDRTWAYGHVVVDEAQELSAMAWR
                     LLMRRCPAKSMTLVGDIAQTGSAAGAHSWAEVLDPYVEGRWRLTELTVNYRTPAQVMT
                     VASAMLEAAGVTAAAPRSVRDGDVPPTAHLIERGDVDAIVALVRAELDELGEGRLAVI
                     APAARVGELQLALATSLPPGLVASGSGALDAPVGVLAVSQAKGLEFDTVVLVEPARIL
                     AASARGANDLYVALTRPTQRLRIVHSEPLPAGLETIPEI"
BASE COUNT          712 a         1254 c         1178 g          536 t
ORIGIN      
        1 ccagagcccc ggcagacgcc gcttaaccga gcgacgaaag ttccgcctcg atcgccgcga
       61 gttcctcggc acttccctgc accttcggac cggtgaacca cttgcgagcg gacaccagcc
      121 agtagatgcc ggcgaacccg atgacagcgg cgacagcggc gggagcgtag ttccagctgt
      181 agggcccggt cggcgaaaac tgcggcagca tgagtgggaa gcagatgagc gctacccaac
      241 ccacggcaag ccagccgatc agcggactcc acttccccaa ctgccacggg ccgggagtga
      301 agtcagaccc gcgcagacga cgaagcagca ccggaatgac gtacgagatg tagagcccca
      361 ccacggcgac ggagacgatg gcgaagaacg cgacagcgac gcccttgtgt gtgtacaagg
      421 acggcacgcc gaggatgaac gcgcccacga cggcgaacca gatcgaattc gttggcgtgc
      481 gggtgcgctt gttcaccttg tgccagaacc tggatccggg gatcgcaccg tcgcgggaaa
      541 acgcgtagat catccgcgag ttggcggtca ccgacgccat gccgcagaag aactgcgcga
      601 cgaggcagac caggatcagg acctcggccg cgtgccgccc gatcgacgcg acccagagca
      661 cccccggagc gttgataccg gcgccggccg cggctgagta agtggcaccg ccgatggcac
      721 cggggatcgc gtacgtgagc ccgatcatca acacgagacc ggcaacaacg gacacccaga
      781 tcgaggtcat gatgccgcgc ggcccgctgg tggccgcatc gcgtgtttct tccgtcatgt
      841 gggcggaggc gtcatatccg gtcagggtgt actgcgccat catcaggccg acgagaacca
      901 cccagatcgc gtggccgccg aaatgccagc cgagaccgtc gtagtacttc gtgaacacgt
      961 tcgacaacga ctggtgcttg tgcgcgtcag ggaggatgaa cagcgcgccg acgatgacga
     1021 cgacgccggt gaggtgccac cacacgctga tgtcgttcag ccgcgcgatg acattgacgc
     1081 ccatcgtgtt caggatgccg tgcacgacga ggacgatcgt gtagatcacg aacaggctct
     1141 tgggcgtcgc gttgaacccg gttgtgaggt tcaggaagaa gccgaggaac gtcgccatcc
     1201 cgaagtcgat cgacgcggtg acggcgacct gcccgagcag attgaaccag ccggtgaacc
     1261 aactccacac gggagccttg ccctcaggtg cgagtttcgc cgcccagtag tagaggccgc
     1321 ccgcagtcgg aaagctcgag cacacctctg ccatgcccgc gccgaccagc atcaccatga
     1381 gcccgacgaa cagccagccc cagacaatga cccgcggccc gccggcatac ataccgaggt
     1441 agtaggtggt gatgccgccg gtcaggatcg agatgatcgt gaacgagacg gcgaagttgg
     1501 agaaccagcc catgccacga tgaagctcct gggcatagcc aagttcgtgc aaccgcttct
     1561 cgtctgcatt caaaacggca acgccgggcg aacttgtggg acgagactcc gtggactcgg
     1621 acatcccgct tccttcctcg aggggaggct ggcttattgg tctggtagcg gaccatagcc
     1681 cgacccactg cggacggcaa ggggtaccga gaacgcgaac gttcgcgtca gcaagcgagc
     1741 ggcgcaagtg tcggccagta cgactaggcg cgtgtcggcg ccgatcgcga cagggcccgc
     1801 tcggtggcga ggttgacgaa cgcttcgaag agccggcgat catcgagttg ctcgggatgc
     1861 cattggacgc cgaacaccgg ccggccgtcg gcagcttcga gcgcctccgg cgtgccgtcg
     1921 tcggctcgtc cgacgatccg aagccctggc gccacccggt cgacggcctg gtgatggtga
     1981 cacgccgtgg gcacgcccgc acctagcgtg cggccgatcc agctatccga gtcgagcgtc
     2041 accgctcgcg tgctgaaaac accacggccc tctgcgtgcg gtaaaacccc ttcaatctca
     2101 ggcaaatgct ggatcaggtc tccgccgagc gcgacgttga gcacctgtaa cccgcggcag
     2161 atgccaagca ccggcacgcc ttgttcgacg gcagcgcgga cgagcgcgaa ctcggcgttg
     2221 tcgcggtcgg cccgaaaggg acccgccgcc gggtgggctt ccgcgtcgta ctccgccgga
     2281 tcgacatctg caccgccgct gatcaacaga ccgtcgagtg cgcgcagcac gtcacccact
     2341 cccgagtccg caggaggcac caagaccaca gcacctccac tcgcggcgac cgcgtcggtg
     2401 tacagggtcg actggatgtc cgccaaccgg tcccacacgc cccagcgggc attctcgcgg
     2461 tacgtcgtga gcccgataat cggtcttccc atgacactcg tccttcggtc gctcacgctg
     2521 tcacgcaggt cgtcaaaagg tatcgcagca gaccattcag atctcgggaa ttgtttcgag
     2581 ccccgctggc aacggctcgc tatgcacgat ccggagccgt tgagtaggtc gggtcaaggc
     2641 gacgtagaga tcgttcgccc cgcgggccga cgccgcaaga atacgggccg gctcgaccag
     2701 cacgacggtg tcgaactcca aacccttagc ctggctcacc gcgagcaccc cgaccggcgc
     2761 gtcgagggcc ccagaaccgc tcgccaccag cccgggcggc aacgacgtgg ccagcgcaag
     2821 ctggagttca ccaacacgcg cggccggcgc gatgaccgcg agcctgcctt cgccgagttc
     2881 atcgagttcg gcgcgcacca aggcaacgat cgcgtcgacg tcaccgcgct cgatcaggtg
     2941 agcggtcggg ggcacgtcgc catcgcgcac cgagcgcggc gctgccgcgg tgacgccggc
     3001 agcctcgagc atggcggacg ccacggtcat gacctgggcg ggcgtgcggt agttgaccgt
     3061 caactcggtc agccgccagc gcccctcgac gtaggggtcc aagacctcgg cccatgaatg
     3121 cgcgcccgcc gccgagccgg tctgcgcgat atcgccgacc agcgtcatcg acttcgccgg
     3181 gcaccgacgc atcagcagcc gccacgccat cgccgacagc tcctgcgcct cgtcgacgac
     3241 gacatgcccg taggcccagg tccggtcgac cgcagcgcgc tcggcagcgc ttaggatcgg
     3301 accctcgtcg gcgaaccgac cagcgagcgc ctcggcactg acgagcccac ctgcgtcgcc
     3361 ggaaatctgc aacgcaccct tcgcgtactc aacttcggac tgccgttgct gggccgcctg
     3421 tctcttgagt tcatagccgc cggtgtcatc gtcgccaaga agttccgcag cttcgtcgag
     3481 caacggaaca tcggagacag tccatggggc accgcggtcg cggcggagca acgcccgctg
     3541 cacgtcgtcc agatgaggtg ctgcggcggc cagccgatgc ggctcggcgt acagaccttc
     3601 cagcaagctc tgcggcgtca taggcatcca ggtcaggttg acctcccgtc gcacgtccgg
     3661 cgattcgcgc aggtcggcca
//
LOCUS       DATCBJ010000179         3316 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_267045, whole genome shotgun sequence.
ACCESSION   DATCBJ010000179 DATCBJ010000000
VERSION     DATCBJ010000179.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 3316)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3316)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3316
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_267045"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..498)
                     /locus_tag="VII50_03600"
     CDS             complement(<1..498)
                     /locus_tag="VII50_03600"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY5049960.1"
                     /translation="MRVVIAGAGFGGLELATRLSEEFGDDADVVLIDRADGFVFGFSK
                     LDVMFGRATAEHVLHRYVDVAGPGVQFVQSTVRVLDPVGRRAVTDDGTFEADVIVVAL
                     GADLDPAATPGLVEGGHEFYTVAGAFAARDALVQFGGGRIVIGVTSTPFKCPPAPSET
                     ALLSHG"
     gene            complement(536..1174)
                     /locus_tag="VII50_03605"
     CDS             complement(536..1174)
                     /locus_tag="VII50_03605"
                     /inference="COORDINATES: protein motif:HMM:NF014069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEY5049961.1"
                     /translation="MTASSPVLPGLPDSPPARAALDLIRANESAAIANHSIRTFLFGM
                     LMADHRGVTDFDPALLFFSCVLHDIGLSELGNRTQRFEVDGADVAADFLAEQGVSAVD
                     RGLVWEAIALHTSGGIAERRGPVTDLTRRGVGMDFGRDVECVSDAQAALIHAVYPRLS
                     IERVLADEIVGQAQARPEKAPRYSIAAELLRERTTVGTTALEQGAAISRWGA"
     gene            1245..2180
                     /locus_tag="VII50_03610"
     CDS             1245..2180
                     /locus_tag="VII50_03610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009084832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEY5049962.1"
                     /translation="MRPHRIAILALDGVLPLDFGIPAHVFLPRLWLPYRTTVCAERPQ
                     VEVATGYSLVVPGTLADVRRADTVIVPGFVDHGRVFSDDVLDALRHVHRRRRRVVSIC
                     TGAFALAAAGLLDGLQATTHWRDIDELAERYPQILVDHNVLYIDNGQVLTSAGVASGI
                     DLCLYIVRRDLGAAVANRLARLIVAAPHRDGGQTQFIEAPVGTTDGSLSATRSWALDH
                     LRDPLSVRDLARHAGVSERTFARRFVDETGSPPLQWLLAARIQRARELIETADLSVDQ
                     VAHHCGMGTAANLRLHFRRFVGTTPTSYRKAFTAT"
     gene            complement(2177..2944)
                     /locus_tag="VII50_03615"
     CDS             complement(2177..2944)
                     /locus_tag="VII50_03615"
                     /inference="COORDINATES: protein motif:HMM:NF024110.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY5049963.1"
                     /translation="MRAALAMEWEKLRPVHKTSRTIALAIALAVTVSALVALAIASSA
                     SHMSSQDRLTFDSVGISLEGVNAAVLAVAAFGILSITREYATGMMAVTFLAQPARLRV
                     LAAKLLTHAGVAGLAGAGACVAAFAVGQTLLSTGGLATGWSAPELPAALGGGVVYLML
                     VCVWGIAIGALVRTSAAAITLSAFLLVVAPILVQVLPESMVQQASRWLPSQIGVQAMS
                     ARHDPHAFAPWTGLAVLAGYVAITLIAGGIRMVRREP"
     gene            complement(2951..>3316)
                     /locus_tag="VII50_03620"
     CDS             complement(2951..>3316)
                     /locus_tag="VII50_03620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY5049964.1"
                     /translation="VASHLMSEMALTASALIVIGAGRLIAAESVTDFVERAVSSVRVR
                     SPQLDDLEAALSLEEASLWRDGDALDVVGLSIEQIGVTASRIGATLFELSPQRASLED
                     AFMQLTCDAAEFATRRQAS"
BASE COUNT          615 a         1176 c         1054 g          471 t
ORIGIN      
        1 accgtgcgac agcagcgcag tttcgctcgg cgccggcggg cacttgaacg gcgtggatgt
       61 cacaccgatc acgatgcggc cgccgccgaa ctgcaccaat gcgtcgcggg cagcgaacgc
      121 acccgcaacg gtgtagaact cgtgcccgcc ctcgacaagc ccgggcgtcg ctgcaggatc
      181 gaggtcggca ccgagagcga caacgatgac gtcggcctcg aaggtgccgt cgtccgttac
      241 cgcgcgacgg ccgacaggat ccagaacgcg aaccgttgac tgcacaaact gcacgccagg
      301 cccggccaca tcgacatacc gatgcaagac gtgctcggcg gtcgcgcggc cgaacatcac
      361 gtcgagcttg gagaacccga agacgaaacc atcggcgcga tcgatcaaga cgacgtcggc
      421 gtcgtcgccg aactcctcgg acaacctggt cgcaagttcg agcccaccaa agccggctcc
      481 ggcgatcacc acgcgcatcc gcgcagccta gagcccactt aggaggatga gtaactcagg
      541 cgccccagcg ggaaatggcc gcgccctgct cgagggcggt cgtccccacc gtcgtgcgct
      601 cgcgcagcag ctcggcggcg atcgaatacc ggggtgcctt ctccgggcgt gcctgtgcct
      661 gcccgacgat ctcgtcggcg agcacgcgct caatcgaaag ccgcgggtac accgcgtgaa
      721 tgagggcggc ctgcgcgtcg ctgacacatt cgacgtcgcg gccgaagtcc atacccactc
      781 cacgacgggt caggtcagtc accggaccgc ggcgctcggc gatgccgccg gaggtgtgca
      841 aggcgatcgc ctcccacacc aggccacgat cgacggccga aacgccttgc tcggcaagga
      901 aatcagcagc aacgtcggcg ccgtccacct cgaaccgctg ggtccggttg ccgagttcgc
      961 taagcccgat gtcatgcagg acgcaggaga agaacagcag cgccgggtcg aagtcggtca
     1021 cgccgcgatg gtcagccatg agcatcccga agagaaaggt tcggatgctg tggttggcga
     1081 tcgcagcact ctcgttggcg cggatcaggt cgagggcggc gcgcgcgggc ggcgagtcgg
     1141 gcaggcccgg cagcaccgga gaggatgcag tcatggctcg atcatggaca taacgacgtc
     1201 agacggcgag tggcaggatc gccacagttc gctagattac cgccatgcgt cctcaccgca
     1261 tcgcgatcct cgctcttgac ggtgtgctgc cgctcgactt cggcattccg gcccatgtct
     1321 tcctgccgcg gctgtggctg ccgtaccgca ccaccgtgtg cgccgagcgc ccccaggtcg
     1381 aggtcgcgac gggctactcc ctcgtcgtac caggcactct cgcggatgta cggcgggccg
     1441 acactgtcat cgtgcctggc ttcgtcgatc acgggcgggt cttctccgac gacgtcctcg
     1501 acgccctcag gcacgtgcac cggcgacggc ggcgcgtcgt atcgatctgc acgggcgcct
     1561 tcgccctcgc ggccgccggc ctactcgacg gcctacaagc gacgacgcac tggcgcgaca
     1621 tcgacgagct cgcggagcgc tacccgcaaa tcctcgtcga ccataacgtc ctgtacatcg
     1681 ataacggcca ggtgctcacc tcggcaggcg tcgcgagcgg catcgacctg tgcctctaca
     1741 tcgtccgccg cgacctcggc gccgccgtcg cgaaccggct ggcgcggctg atcgtcgcgg
     1801 cgccgcaccg agacggcggc cagacacagt tcatcgaggc accggtgggg acgaccgacg
     1861 ggtccctgtc ggcgacccgg tcatgggcac tcgaccatct cagagatccg ttgtcggtac
     1921 gagacctcgc ccggcacgcc ggtgtctccg agcgcacgtt cgcacggcgc ttcgtcgacg
     1981 agacgggatc gccgccgctg caatggctgc tcgccgccag gatccagcgt gcccgcgagc
     2041 tcatcgagac cgccgacctc agcgtcgacc aggtcgcaca ccactgcgga atgggcacgg
     2101 ccgccaacct gcgcctgcac tttcgccgct tcgtcggcac caccccgacg tcctaccgca
     2161 aagcgttcac cgcaacttag ggttcgcggc gaaccatccg gatgccaccg gcgatcaacg
     2221 tgatcgccac atacccggcc aacacggcga ggccggtcca cggggcgaat gcatgcgggt
     2281 cgtgacgtgc cgacatggcc tggacgccga tttgtgacgg gagccaccgc gatgcctgct
     2341 gcaccatgct ctccggcaga acctgcacca ggatgggcgc cacaacgagc aggaaagcac
     2401 tcaacgtgat cgcggcagcc gacgtgcgca ccaacgcacc aatcgcgatg ccccacacgc
     2461 acacgagcat gaggtagacg actcccccgc cgagcgcggc tggcagttcc ggtgctgacc
     2521 atccggtggc aagaccgccg gtactcagca gtgtctgccc caccgcgaac gcggccacgc
     2581 acgcaccggc accggccaac ccggcaacgc cggcgtgggt gagcagcttc gcggccaaca
     2641 cccggaggcg tgcgggctgc gcaaggaagg tgacggccat catcccggtg gcgtactcgc
     2701 gagtgatcga caagattccg aatgccgcaa cggcgagaac cgcagcgttc acaccttcga
     2761 gcgagatgcc cactgagtcg aatgtgagcc ggtcctggct ggacatgtgc gaggcagaag
     2821 acgcaattgc cagtgcaacg agggcgctga ccgtcaccgc cagcgcgatc gcgagagcga
     2881 tcgtgcggga ggtcttgtga acgggccgca gcttctccca ctccatcgcc agcgcagcac
     2941 gcatcgacgc tcagctcgcc tggcgccggg tggcgaactc ggccgcatcg caggtcaact
     3001 gcatgaaggc gtcttccaac gacgctcgct gcggcgagag ctcgaacaac gtggcaccga
     3061 tccgagaagc ggtcacaccg atctgctcga tgctgagccc gacgacgtca agagcgtcgc
     3121 cgtcgcgcca caatgacgcc tcctccagag atagcgcggc ctcgagatca tcgagctgcg
     3181 ggctgcgcac ccgcaccgaa ctcactgcac gctcgacgaa gtcggtcaca ctctcggccg
     3241 cgatcaagcg gcccgcaccg atcacgatca gcgcgcttgc ggtcaacgcc atctcgctca
     3301 tcaggtggct cgcgac
//
LOCUS       DATCBJ010000180         3698 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_82420, whole genome shotgun sequence.
ACCESSION   DATCBJ010000180 DATCBJ010000000
VERSION     DATCBJ010000180.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 3698)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3698)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3698
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_82420"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1049)
                     /locus_tag="VII50_03625"
     CDS             complement(<1..1049)
                     /locus_tag="VII50_03625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5049965.1"
                     /translation="MMARVENEFREFVVGFADPLTRLAFLLTAGASGDPAVLSANALA
                     RVRRQWRDAEMTGAPESLAVEALLSALPHRRQFHLHGHAGPEEPDDSDFVLATANAAS
                     GAAVNHDAFRRPVTNDERPTSSPLDDAVEGVAGGAVDLDVLHDGVWTAWQRLSPRQRV
                     PLVFADPSVASRRLVGLGVPESFGSPRRLDLIASGALAQLRNALAADPTIGVAVGPMR
                     DRQFADVLADTLREHGSTPSRVIDPYPVVVRSAQRLGRRAVVAVAAVLAVLATGFVVA
                     VQASQPTKATSAAAAAAASRSGSPTLVNTGPSPTFTGGLSPQGPFAPAARPSGPVLVV
                     PWSPRGNAANDSALLS"
     gene            complement(1062..2909)
                     /locus_tag="VII50_03630"
     CDS             complement(1062..2909)
                     /locus_tag="VII50_03630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5049966.1"
                     /translation="MTRSEQEFRDFVAGFANPLARLAFLMSAGTELNSVDMTVAALAA
                     VRRRWRDVEATGAPEPLAVEALVSALPRLRRAAPAVPAIDQLSDDEADSGLLRAAAWT
                     AWKTLPPRERVPFLLDDPSVAAQRLAGIDVPQSSRSARRAAVVADGARRILRASMVSD
                     AVVRRWVVSRGDEDFAALVRDALREQVATAPTLLEPHARVMQTAYRVRLRAAGAVATS
                     VVVLVGAAAVAIRASAPNKDKIPPVSASAEADSVSDRGPVVAWPTRGTASHNSTLTSK
                     LKAAFLHAHPETTGQVQVLLITDTSSYRLAYVTAKSPTGVLESWFYGPVGSNDLVEGA
                     VQYGAGLDARTEMLATGVADAQGHTQLVVLAPPGTRTMRLSTSAGATAPASPSTLRDA
                     DGIAVKAVATGSIASLRLDVRIGQTDVVSKEMRTLALGPTFQGVGAPFEPEETPSFPS
                     ERGHPDPQVLAQAAYDAGVWAVQDGMANRVQMKVLWGGTDQAGNRIVVVRMKMELSDM
                     LLVTWDDESNVPRQETADIADPSTPDAPLAFSYVGSDGPRVAVLGAPGDRTAELAFQG
                     KQLPAVPLDGSGFASFRVADDGWLTSPGLAVGLLGPAGNVLKTIPIPAL"
     gene            complement(2918..>3698)
                     /locus_tag="VII50_03635"
     CDS             complement(2918..>3698)
                     /locus_tag="VII50_03635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="2-hydroxyacid dehydrogenase"
                     /protein_id="HEY5049967.1"
                     /translation="LELARTMESLQVLQLLTAGYEHVLPYVPDGVQLCNARGVHDSST
                     AELALTLMLSSLRGIPDFVRAQQHGNWVHADYPALADKTVLILGYGSIGEAIERRLIP
                     FEVNVIRVASRARPDGVHGVDELPELLPQADVVVVITPLTPATRHLVDGPFLAAMRDG
                     ALLVNVSRGGVVDTDALLTELLRGRLRAALDVTDPEPLPSDHPLWTAPGVLISPHVGG
                     NSSAFLPRAALLVRRQVAKFVAGEPVDNVVAGPASPIALGA"
BASE COUNT          636 a         1293 c         1237 g          532 t
ORIGIN      
        1 gagagcaggg cgctgtcgtt ggccgcgttt cccctcggcg accacggcac caccaagacc
       61 gggccactgg gtcgcgccgc gggggcaaac ggcccctgtg gggaaagacc gccggtaaag
      121 gttggcgatg gtccggtgtt gacgagcgtc ggcgaaccgc ttcttgaggc ggccgcagcc
      181 gcagcagccg aggtcgcctt cgtcggctgc gacgcctgca cggcgacgac gaacccggtc
      241 gccagaacgg ccagaaccgc ggcgacggca acgacggccc gacgacccag acgttgcgca
      301 ctgcgcacca cgacgggata cggatcgatc acccgcgaag gcgtcgatcc gtgttcacgc
      361 aacgtgtcgg caaggacgtc agcaaactgg cggtcacgca tggggccgac ggccaccccg
      421 atcgtcgggt cggccgcgag cgcgtttcgc aactgcgcca aagcgcccga ggcgatgagg
      481 tcgagtcggc gtggcgaacc aaacgattcg gggacgccga gcccgaccaa tctccgcgac
      541 gctaccgagg ggtccgcgaa caccagcgga acccgctggc gcgggctcag tcgctgccac
      601 gccgtccaca cgccgtcgtg tagcacgtcg aggtcgacgg caccgccagc cacaccctcg
      661 accgcgtcgt cgagcgggct actcgtggga cgttcgtcgt tcgtgaccgg acgccggaac
      721 gcgtcgtgat tcaccgcggc gccgctggcc gcgttagccg tggcgagaac gaaatcggag
      781 tcgtccggct cctccggacc cgcgtggccg tgcaggtgga actgtcgacg atgcggcagc
      841 gcggacagca gggcctcgac ggcgagtgac tccggagcac cggtcatttc ggcgtcgcgc
      901 cattgccggc gtacccgagc gagcgcattc gcgctcagca cggctgggtc gcccgacgcg
      961 ccggctgtga gcaggaaagc aagccgtgtg agggggtcgg caaagccgac gacgaactca
     1021 cggaactcgt tctcgacccg tgccatcaca acagcgtccc tctacaaggc cgggatcgga
     1081 atggtcttga gcacgttgcc cgcagggccg agcaggccca cggctaggcc aggagacgtc
     1141 agccagccgt cgtcggcgac ccggaagctg gcgaagccgg agccgtccaa tggcaccgcc
     1201 ggcaactgct tgccttgaaa tgccagctcg gcggtgcggt cgccgggcgc gccgagtacg
     1261 gcaactcggg ggccgtcgga cccgacgtag ctgaacgcga gcggcgcgtc aggcgtgctc
     1321 gggtcggcaa tgtccgccgt ctcctgccgg ggcacgttgc tctcgtcgtc ccacgtgaca
     1381 agcagcatgt cggacaattc catcttcatc cggaccacca cgatgcggtt gccagcctga
     1441 tcggtaccgc cccacaacac cttcatctgc acgcgattgg ccatgccgtc ctgcactgcc
     1501 cacaccccgg cgtcgtacgc cgcctgcgcc agaacctgcg ggtctggatg gccgcgctcg
     1561 gaaggaaacg atggtgtctc ctcgggttca aacggtgcgc ccaccccctg gaacgtcgga
     1621 ccgagcgcga gggttcgcat ctccttgctg accacatcgg tctgcccgat ccgcacgtcg
     1681 aggcgcagcg acgcaatcga gccggtggcg accgctttga cggcgatgcc gtctgcgtcg
     1741 cgcaaggtcg acggcgatgc cggcgcagtc gccccggcgc tggtgctcaa ccgcatcgtg
     1801 cgggtacctg gcggagcgag caccacgagc tgggtgtgcc cctgtgcgtc agcgactccg
     1861 gtcgcgagca tctcggtgcg ggcgtctaga cccgcgccgt actgcacggc tccctcgacg
     1921 aggtcgttcg agccgaccgg gccgtagaac cacgactcga gcactccggt cggcgacttt
     1981 gccgtgacgt aggcgagtcg gtagctcgac gtgtccgtga tcaggagtac ctgcacctgc
     2041 cccgtggttt ccggatgggc atgcagaaac gccgctttca gcttcgaagt cagcgtgctg
     2101 ttgtgagaag ccgtacctcg tgtcggccat gcaaccaccg ggcccctatc gctgactgaa
     2161 tccgcctccg ccgaggcaga gaccggtggg atcttgtcct tgttcggcgc cgatgcacgt
     2221 atcgcaaccg ccgctgcacc gaccagcacg acaaccgagg ttgccaccgc accggcggcc
     2281 cgcaggcgca cccgatacgc ggtctgcatg actcgcgcat gcggctcgag cagcgtcggc
     2341 gccgttgcca cctgctcgcg caacgcgtcg cgcaccaatg ccgcgaaatc ctcgtcaccc
     2401 cgtgagacga cccaacgacg cacgaccgca tcgctcacca tcgatgcccg gaggattcgc
     2461 ctagcgccgt cggcgacaac cgccgctcga cgcgccgacc gcgacgactg cgggacgtcg
     2521 attccggcca gccgctgtgc cgccaccgac ggatcatcga gcaggaacgg cacccgttcg
     2581 cgcgggggca acgtcttcca agcagtccag gccgccgcac gcagcaggcc tgagtcggcc
     2641 tcgtcgtcgg acaactgatc gatcgcgggg accgcgggtg ctgcccgtcg cagtcgcggg
     2701 agggcggaca ccaatgcctc cacggcgagt ggttccggag ctccggtggc ctcgacgtcg
     2761 cgccatcgcc ggcgaacggc ggcgagcgcc gccacggtca tgtccaccga gttcagctca
     2821 gtaccggcgc tcatcaggaa cgcgagtcgc gcgagcgggt tggcgaagcc agcgacgaag
     2881 tcgcggaact cctgctccga tcgcgtcacg atggcggtca ggcccccaag gcgatcgggg
     2941 aggcgggccc ggccacgacg ttgtcgaccg gctccccggc gacaaatttg gccacctgcc
     3001 tgcgaaccaa cagcgccgcc cgcggcagga acgcggacga gttgccaccg acgtgcggcg
     3061 agatgaggac gccgggcgcg gtccacagcg gatgatcgct tggcagtggt tcaggatcag
     3121 tgacgtcgag tgccgcccgt agccgaccac gcagcaactc ggtcaacagt gcgtcggtgt
     3181 cgacgacgcc gccccgcgaa acgttgacca gtagggctcc gtcccgcatc gccgccagga
     3241 atggaccgtc aacgaggtgt cgcgtcgccg gcgtcagtgg cgtgatgaca acgaccacgt
     3301 cggcctgtgg caatagctcg ggcagctcgt cgacgccgtg cacaccgtct ggcctggccc
     3361 gactcgcgac acggatgacg ttgacctcga acggaatgag ccgccgttcg atcgcctcgc
     3421 cgatcgatcc gtaaccgagg atcagcacgg tcttgtcagc caacgccgga tagtccgcgt
     3481 gcacccagtt gccgtgctgc tgcgcgcgca cgaagtccgg aatcccgcgc agagacgaca
     3541 gcatcaaggt cagcgccagc tcggcggtac tcgaatcatg cacgccgcgg gcgttgcaca
     3601 gctggacgcc gtccggcacg tacggcaaca catgctcgta gcccgcggtt agcagctgaa
     3661 gtacctggag cgactccatc gtgcgggcaa gttcgagc
//
LOCUS       DATCBJ010000181         4852 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_45951, whole genome shotgun sequence.
ACCESSION   DATCBJ010000181 DATCBJ010000000
VERSION     DATCBJ010000181.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 4852)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4852)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4852
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_45951"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            17..1429
                     /gene="sufB"
                     /locus_tag="VII50_03640"
     CDS             17..1429
                     /gene="sufB"
                     /locus_tag="VII50_03640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019633769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HEY5049968.1"
                     /translation="MTTIEHPELDQLGRYKFGWSDSDVAGAAAKRGLNEDVVRDISAK
                     KSEPAWMLEQRLKGLRIFEKKPMPLWGADLSGIDFDNIKYFVRSTEKQADSWDDLPAD
                     IRNTYDKLGIPEAEKQRLISGVAAQYESEVVYHKIREDLEQQGVLFLDTDTGLREHED
                     LFREYFGSVIPAGDNKFSALNTAVWSGGSFIYVPPGIHVDIPLQAYFRINTENMGQFE
                     RTLIVVDEGSYIHYVEGCTAPVYSSDSLHSAVVEIVVKKNARCRYTTIQNWSNNVYNL
                     VTKRAVAQAGATMEWIDGNIGSKVTMKYPAVWLVGEYAKGETLSVAFAGEGQHQDAGS
                     KMVHAAPHTSSTIISKSVARGGGRTSYRGLVQIMDGAYGSKSTVKCDALLIDAISRSD
                     TYPYVDVREDDVSMGHEATVSKVSEDQLFYLMSRGMTEDEAMAMIVRGFVEPIARELP
                     MEYALELNRLIELQMEGSVG"
     gene            1433..2644
                     /gene="sufD"
                     /locus_tag="VII50_03645"
     CDS             1433..2644
                     /gene="sufD"
                     /locus_tag="VII50_03645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HEY5049969.1"
                     /translation="MTELTTTEDLELGELQRAKAEITDAPAAPEGHTHGRGGEGGRHL
                     ERTFSRDPQAFAEPNSRQEIWRFAALPRLRALFAGPDADGPLAYSWSPDAPVQLVPIS
                     DPRVGAVHTPFDRLSAVALRKATEALVVTVAGDVVDPIVVGLKGTGGVAYGHVIVDVA
                     PHATGMVVLDHVGTAQYAANVEIKVGDGASVTVVSIQDWDDDAVHASAHTALVGRDAK
                     LRHINISFGGSAVRVTPLVRFAGPGGDAELLGLFFADAGQHIDNRLLVDHAMPHCKSR
                     VNYRGALQGAGARSVWTGDVIIRTTGIGTDTYEVNRNLLLTDGARADSVPNLEILTGE
                     VVGAGHASATGRFDDEQLFYLQARGIPADQARRLIVRGFFADVIERIQVPELVERLLA
                     TVDRELDSSMS"
     gene            2641..3024
                     /locus_tag="VII50_03650"
     CDS             2641..3024
                     /locus_tag="VII50_03650"
                     /inference="COORDINATES: protein motif:HMM:NF025178.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme iron oxygenase ferredoxin subunit"
                     /protein_id="HEY5049970.1"
                     /translation="MTTRYVKVGERAQLPAPRGVLRVQLEGEPVAIICTEGGSFSAIS
                     DVCSHAEVALSDGDIEGNTVECWLHGSRFDVCTGKPLGLPATRAVPVYDVKFDGDDVY
                     LSSAPSDLSSTSLPDISLNDWSPLR"
     gene            3021..3809
                     /gene="sufC"
                     /locus_tag="VII50_03655"
     CDS             3021..3809
                     /gene="sufC"
                     /locus_tag="VII50_03655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HEY5049971.1"
                     /translation="MSALVIRDLHVVVNAGTTGEAGAIGTGEEPREILRGVDLTVNAG
                     ETHAIMGPNGSGKSTLAYSIAGHPKYTITKGSVTLDGQDVLAMKVDERARAGLFLAMQ
                     YPVEVAGVSVSNFLRTAATAIRGEAPKVRTWVGEVKGAMAQLQIDPAFSERNVNEGFS
                     GGEKKRHEILQLELLNPKFAVLDETDSGLDVDALRVVSEGVNRFRAQPDRAVLLITHY
                     TRILRYIEPDFVHVFVDGRIVEEGGRELADRLEAEGYERYAKTA"
     gene            3855..>4852
                     /locus_tag="VII50_03660"
     CDS             3855..>4852
                     /locus_tag="VII50_03660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEY5049972.1"
                     /translation="MTEVGTTTYDVELLRKDFPILERRVHDNQPLVYLDSANTSQKPR
                     HVLDTLAEFYEQHNSNIHRATHALGEEATAAYEGARLKVAAFIGAPDADTVIFTKNST
                     EALNLVANVMVWGDRGLKPGDEVVVTEMEHHSNIVPWQILCERTGATLRWFGVTDDGR
                     LDVSAIDELINERTKVVSFVHVSNVLGTLNPVAAIVARAHAVGAVVVLDASQSVPQLP
                     VDVAALGIDFAAFTGHKMCGPTGIGVLWGRPELLDELPPFLGGGEMIETVTMQGSTYA
                     GLPHKYEAGTPPIAQAVGLGAAVDYLSAIGMDAVASHEKQITAYALDALSTVSGLR"
BASE COUNT          890 a         1564 c         1553 g          845 t
ORIGIN      
        1 ccggagaggt caccgcatga cgaccatcga gcatccagag ctcgaccagc tcggccggta
       61 caagttcggc tggtccgact ccgacgtcgc gggagcggcc gcgaagcgtg gtctaaacga
      121 ggacgtcgtt cgcgatatct ccgcgaagaa gagcgagccc gcgtggatgc tcgagcagcg
      181 cttgaagggc ctgcggatct tcgaaaagaa gccgatgccg ctctggggtg ccgatctgtc
      241 cgggatcgac ttcgacaaca tcaagtactt cgtgcgctcg accgagaagc aggccgacag
      301 ctgggacgac ctgccggcgg acatccgcaa cacctacgac aagctcggga taccggaagc
      361 cgagaagcaa cggcttattt ctggcgtagc ggcacaatac gaatcagaag tggtttacca
      421 caagattcgg gaagatttgg aacaacaggg cgtattgttc ctcgacaccg acaccggttt
      481 gcgcgagcac gaggacctgt tccgcgagta cttcggctcg gtgattccgg ccggtgacaa
      541 caagttctcc gcgctgaaca ccgcggtgtg gtcgggcggc tcgttcatct acgtgccgcc
      601 gggcatccac gtcgacatcc cgctgcaggc ctacttccgg atcaacaccg agaacatggg
      661 ccagttcgag cgcaccctga tcgtcgtcga cgaaggttcg tacatccact acgtcgaggg
      721 ttgcacggcg ccggtgtact cgagtgactc gttgcacagc gcggtcgtcg agatcgtcgt
      781 gaagaagaac gcgcgctgcc ggtacaccac gattcagaac tggtcgaaca acgtctacaa
      841 cctcgtcacc aagcgtgcgg ttgcccaggc cggcgcgacg atggagtgga tcgacggcaa
      901 catcggctcg aaggtcacca tgaagtaccc ggcggtctgg ctggtcggcg agtatgccaa
      961 gggcgagacg ctgtcggtcg cgttcgcggg cgagggtcag caccaggacg ccggctcgaa
     1021 gatggtgcac gccgctccgc acacgtcgtc gacgatcatc tcgaagtcgg ttgctcgcgg
     1081 cggcggtcgc accagctatc gaggtctggt ccagatcatg gacggcgcct acggctcgaa
     1141 gtcgacggtg aagtgcgacg ccctgctcat cgacgccatc agccggtccg acacctaccc
     1201 ctacgtcgac gtgcgcgaag acgacgtgtc gatgggtcac gaggccacgg tctcgaaggt
     1261 cagcgaagac cagctcttct acctaatgag ccgcggcatg actgaggacg aagcgatggc
     1321 gatgatcgtg cgcggcttcg tcgagccgat cgcgcgcgaa ctcccgatgg agtacgccct
     1381 cgaactgaac cggctcatcg agttgcagat ggaagggtct gtgggctgat ccatgactga
     1441 gctcaccacg accgaggatc tcgagctcgg cgaactccag cgcgccaagg cggagatcac
     1501 tgacgcgccc gcggcgcccg aaggccacac gcatgggcgc ggcggcgaag gcggccgcca
     1561 tctcgagcgg acgttctcgc gcgacccaca ggcgtttgct gaaccgaaca gtcgccagga
     1621 gatctggcgg ttcgctgcgc tgccccgcct ccgcgcgttg ttcgcagggc cggacgccga
     1681 tgggccgctc gcttattcat ggagccccga cgcccccgta cagctcgtcc cgatcagtga
     1741 tccgcgggtg ggtgctgtgc acacgccgtt cgaccggttg tcagccgtcg cgctccgcaa
     1801 ggcaacggaa gcgctggtgg tcaccgtcgc tggtgacgta gtcgatccca tcgtggtcgg
     1861 tctgaaaggc accggcggcg tcgcctacgg gcacgtcatc gtcgatgtcg cgccccacgc
     1921 gaccggcatg gtcgtcctcg accacgtcgg tacggcgcag tacgccgcca acgtggagat
     1981 caaggtcggc gatggcgcgt cggtcactgt ggtcagcatc caggattggg atgacgacgc
     2041 cgtccacgcg tcggcacaca ccgcgctcgt cggacgcgac gcgaagctgc ggcacatcaa
     2101 catcagcttc ggcggcagtg ccgtgcgcgt cacgccactc gtgcgtttcg ccggccccgg
     2161 cggcgacgct gagttgcttg gcctgttctt cgccgatgcc ggtcagcaca tcgacaaccg
     2221 cttgcttgtc gatcatgcga tgccccactg caagagccga gtcaactatc gcggcgcact
     2281 gcaaggcgcc ggtgcacgtt cggtctggac cggcgacgtc atcatccgca ccaccggcat
     2341 cggtaccgat acctacgagg tcaaccgcaa tttgctgctc accgacgggg cgcgtgctga
     2401 ctcagtgccg aacctcgaga tcctgaccgg cgaagttgtc ggcgccggtc atgcgagtgc
     2461 aaccggccga ttcgacgacg agcagctttt ctacctgcaa gcgcgcggca ttccggccga
     2521 ccaggcgcgt cgcttgatcg tgcgtggctt tttcgccgat gtcatcgagc ggatccaagt
     2581 acccgagctg gttgagcggt tgctcgcgac cgtcgaccgc gaactcgata gcagcatgtc
     2641 gtgaccaccc gttacgtcaa ggttggcgaa cgtgcccaac tgccagcgcc gcgcggtgtg
     2701 ttgcgtgtcc agctcgaggg cgagccggtc gcgatcatct gcaccgaggg tggcagcttc
     2761 tcggccatct ccgacgtgtg ctcgcacgcc gaagtcgcgt tgtctgacgg cgacatcgag
     2821 ggtaataccg tcgaatgctg gctgcacggg tctcggttcg acgtgtgcac gggcaagccg
     2881 cttggcctac ccgcaactcg cgcggtgccg gtctacgacg tcaagttcga tggcgacgac
     2941 gtgtacctga gcagtgcccc ctccgacctc tcttctactt ctctgcccga catttcactc
     3001 aatgactgga gcccgttgcg atgagcgcgc ttgtaatccg cgacctgcat gtcgtcgtca
     3061 acgccggtac cactggcgag gccggcgcga tcggcaccgg cgaggagcca cgcgagatcc
     3121 tgcgcggcgt cgacctcacg gtgaatgcgg gcgagacgca cgccatcatg ggccccaatg
     3181 gctcaggcaa gtcgacgctg gcgtactcga ttgccggcca cccgaaatac accatcacca
     3241 agggctcggt caccctcgac ggccaggacg tactggcgat gaaggtcgac gagcgcgccc
     3301 gcgctggcct cttcctcgcg atgcagtatc ccgtcgaagt cgccggtgtc tcggtgtcga
     3361 acttcctgcg cactgcggcc accgcgatcc gtggagaggc acccaaggtc cgcacctggg
     3421 tcggcgaggt caagggcgcc atggcgcaac ttcagatcga cccagccttt tccgagcgca
     3481 acgtgaacga gggattctcc ggcggcgaga agaagcggca cgagatcctt cagctcgagt
     3541 tgctcaatcc taagttcgcg gtgctcgacg agaccgactc cgggcttgac gtcgatgcac
     3601 tgcgcgtcgt gtccgagggc gtcaacaggt tcagggcgca accagatcgt gccgttctgc
     3661 tcatcacgca ctacacccgc atcctgcgct acatcgagcc cgacttcgtg catgtgttcg
     3721 tcgacggccg gatcgtcgaa gaaggcggcc gcgagcttgc cgaccggctc gaggccgagg
     3781 gttacgagcg atacgcgaag accgcgtgag gaggcgctgt tcccatgaat agtgttcgta
     3841 gtaatagtgt tccgatgact gaggtcggca cgaccaccta cgacgtcgag ctgctgcgca
     3901 aggacttccc gatccttgag cggcgggtcc acgacaatca gccgctggtc tatctcgaca
     3961 gtgcaaacac ctcgcagaaa ccacgccacg tgctcgacac gttggccgaa ttctacgagc
     4021 agcacaactc caacatccac cgtgcgacgc acgcactggg cgaagaggcg accgcggcgt
     4081 atgagggcgc tcggctgaag gtggccgcat tcatcggggc gccggacgcc gacaccgtca
     4141 tcttcaccaa gaactccacc gaggctttga acctggtcgc gaacgtgatg gtgtggggag
     4201 accgcgggtt gaagcctggc gacgaagtcg tcgtcaccga aatggagcac cactccaaca
     4261 tcgtgccgtg gcagatcctg tgtgagcgca ctggggcgac cctgcgctgg ttcggtgtca
     4321 ccgacgatgg ccggctcgat gtctccgcta tcgacgagct gatcaatgag cgcacgaagg
     4381 tggtctcgtt cgtccacgtg tccaacgtgc tcggcacgtt gaatccggtc gccgcgattg
     4441 tcgcgcgggc gcacgctgtc ggtgccgtag tcgtgctcga tgcctcgcag tcagtgcccc
     4501 agcttccggt cgacgtcgcc gcgctcggta tcgatttcgc cgccttcacg ggacacaaga
     4561 tgtgcggccc gaccggcatc ggcgtgttgt ggggacgccc cgaactactc gacgagttac
     4621 cgccgttcct cggcggcggc gagatgatcg agaccgtcac gatgcagggt tcgacgtacg
     4681 ccgggctgcc gcacaagtac gaggcaggca ccccgccgat tgcccaggcg gtcgggctcg
     4741 gcgcggcagt cgactacctg agcgccatcg gcatggacgc cgtcgcgtca cacgagaagc
     4801 agatcaccgc ctatgccctc gatgcgttga gcacagtgtc cgggctgcgg at
//
LOCUS       DATCBJ010000182         3223 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_228106, whole genome shotgun sequence.
ACCESSION   DATCBJ010000182 DATCBJ010000000
VERSION     DATCBJ010000182.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 3223)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3223)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3223
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_228106"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..722
                     /locus_tag="VII50_03665"
     CDS             <1..722
                     /locus_tag="VII50_03665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEY5049973.1"
                     /translation="QFPPREGTIVLVVGFPTGLFAANCYVVAPSAGERCVVIDPGQDA
                     VPDLTEVLREHRLRPAAVLVTHGHIDHMWSVLPVCGAHDVAAYIHGSDRDRLADPVRT
                     LSAELVEALGVAGVTFREPDDVRELTDGEVLDVAGLTMTADHTPGHTEGSVVFRLPEG
                     GPDGEPLMFSGDLLFAGSIGRTDNAGGDDAAMQTSLARVLTFADETVVLPGHGRSTTI
                     GRERATNPHLRGLPVVREARS"
     gene            719..1978
                     /gene="hisS"
                     /locus_tag="VII50_03670"
     CDS             719..1978
                     /gene="hisS"
                     /locus_tag="VII50_03670"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: protein motif:HMM:TIGR00442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HEY5049974.1"
                     /translation="MSTIDAPRGVKEYLPPESAAFLAVRDALSAPAVAAGYGYIELPT
                     FEHTELFVRGVGESTDVVSKEMYTFDDRGGRSVSLRPEGTAGVMRAVIQHGLDGGALP
                     IKLWYAGPFFRAESPQLGRYRQFFQVGVEAIGVDDPALDAEVIAIGDDGFRSLGLTDF
                     ALEITSLGDAKCRPAYRAKLVAFLDTLDLDETTRERARLNPLRVLDDKRPEVRGQLDG
                     APLIVNHLCAECADHFAAVRAYLDALRVHYTLNPRMVRGLDYYTKTTFEFVHHGLGSQ
                     SGIGGGGRYDGLMAELGGQALSGIGFGLGIDRALLACHAEGRDVGATPRVSVFFVPLG
                     DAARVESIATMGKLRRAGVHADLAYGSLSLKGAMKAADRSGAAHAVVIGDRDLHAGVA
                     QVKDLVTGEQSAVALTDLVASLKEKFL"
     gene            1975..>3223
                     /gene="aspS"
                     /locus_tag="VII50_03675"
     CDS             1975..>3223
                     /gene="aspS"
                     /locus_tag="VII50_03675"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HEY5049975.1"
                     /translation="MIRTREAGTLRRSDAGTTVTLAGWVARRRDLGGVAFVDLRDASG
                     IAQVVFREGAVAVAAERLRAEFCVRVVGEVRERPAGNDNAELATGEIEVVATELEVLS
                     EAAPLPFPIAGNADINEEARLKYRYLDLRRDGPAAALRLRSQANRVARDVMAKHRFVE
                     VETPNLTRSTPEGARDFLVPVRLQPGHWYALPQSPQLFKQLLMVAGMERYFQIARCFR
                     DEDFRADRQPEFTQLDIEMSFIDQDDIIALGEDIVAALWREILGYEIPLPIARMTYAD
                     AMNRYGSDKPDLRFGNELIDCTAFFASTPFRVFQAPYVGAVVMPRGASQTRKELDAWT
                     DWAKSRGAKGLAYVLVADDGSLGGPVAKNLSDAELAGLAGHVAAAPGDAVFFAAGERS
                     PSLELLGAARLEIGRRCGLIDESL"
BASE COUNT          495 a         1056 c         1113 g          559 t
ORIGIN      
        1 tccagtttcc gccgcgagaa gggacgatcg tgctcgtcgt cgggttcccg accggccttt
       61 tcgcggccaa ctgctatgtc gtcgccccat cagcgggcga gcggtgtgtc gtcatcgatc
      121 cggggcagga tgcggtgccc gaccttacgg aggtcctgcg cgaacaccgg ctccggccgg
      181 ccgctgtgct ggtgacccac gggcacatcg accacatgtg gtcggtgctg ccggtatgcg
      241 gggcgcacga cgtcgcggcc tatatccacg gcagcgatcg tgatcgcctc gccgatcccg
      301 tgcgcaccct ctcggctgag ctggtcgagg ctctcggcgt cgcgggcgtg acgtttcgcg
      361 agcccgacga tgttcgggag ctaaccgatg gcgaggtcct cgacgtggct gggctgacca
      421 tgaccgcaga ccacacccca ggtcacaccg agggatccgt ggtgtttcgg ctgcccgagg
      481 gcgggccgga cggcgagccg ctgatgttct ccggagacct gctgttcgcc ggctcgatcg
      541 gccgcaccga caacgccggc ggcgacgacg cggcgatgca gacgagcctt gcgcgggtgt
      601 tgacgtttgc cgacgagacc gtcgtgctgc cggggcacgg tcgatcaaca accatcggcc
      661 gcgaacgggc aacgaacccg cacctgcgcg gtctgccggt cgtccgtgag gctcgatcgt
      721 gagcacgatc gacgcgccca ggggcgtgaa ggagtacctg ccaccagaat cggcggcgtt
      781 cctcgcggtc cgcgatgcgc tgagcgcgcc ggcagtcgca gccggctacg gctacatcga
      841 gctgccgacg tttgagcaca ctgagctttt cgtgcgcgga gtcggtgagt cgaccgacgt
      901 cgtgagcaag gagatgtaca ccttcgacga tcgcggcggc cggtcagtgt cgttgcggcc
      961 ggagggcacc gctggggtga tgcgcgcagt catccagcac gggctcgacg gcggcgcgct
     1021 gccgatcaag ctctggtacg cggggccgtt cttccgtgcg gagagcccac agctcggccg
     1081 ttaccggcag ttcttccagg tcggtgtcga ggccatcggt gtcgacgatc cggctctcga
     1141 cgccgaggtc atcgcgatcg gcgacgacgg gttccggtcg ctggggctca ccgacttcgc
     1201 gctcgagatc acctcgctcg gcgatgcgaa gtgccggcct gcctatcgcg cgaaactcgt
     1261 cgccttcctc gacacgctcg acctcgacga gacgacccgc gagcgagcgc ggctgaatcc
     1321 cctgcgggtg ctcgacgaca agcggcccga ggtccgcggc caactcgatg gtgcgccgct
     1381 catcgtcaat cacctgtgcg ctgagtgcgc cgatcacttc gctgcagtgc gggcctacct
     1441 cgacgcgcta cgagtgcact acacgctgaa cccacgcatg gtgcgcgggc tcgactacta
     1501 cacgaagacg acgttcgagt tcgtgcacca cggccttggt tcgcagtcgg gcatcggcgg
     1561 cggcggacgc tacgacgggc tgatggcgga gcttggcggg caggcgttgt cgggcatcgg
     1621 tttcggtctc ggcatcgatc gcgccctcct cgcgtgccac gcggagggtc gcgatgttgg
     1681 cgcgacgccc agggtctcgg tcttcttcgt accccttggt gatgccgctc gggtcgaaag
     1741 catcgccacg atgggaaagc tacgcagggc aggcgtgcac gcggatcttg cgtacggcag
     1801 cctgagtctg aaaggcgcga tgaaggcggc cgaccgatca ggtgccgctc atgcagttgt
     1861 catcggcgat cgcgatctgc acgccggggt cgcccaagtg aaggatctgg tgactggcga
     1921 gcaatccgcc gtcgcactca ccgatctcgt cgccagcctg aaggagaagt tcctgtgatc
     1981 cgcacccgcg aggccggcac gctgcgccga tccgacgccg gcacgacggt gaccctggcc
     2041 ggatgggtgg cccgccgtcg cgaccttggc ggcgtggcgt tcgtcgatct gcgtgacgca
     2101 tcaggaattg cgcaggtggt tttccgggag ggcgccgtgg cggtagcggc cgagcggttg
     2161 cgtgcggagt tttgcgtgcg ggtcgtcggt gaagtgcgcg aaaggcccgc tggcaatgac
     2221 aatgccgaac tcgccaccgg tgagatcgaa gtcgtcgcca ccgaactcga ggtgctctca
     2281 gaggcagcgc cgctgccgtt cccgatcgcg ggcaatgccg acatcaacga agaggcacgg
     2341 ctgaagtacc gctacctcga tcttcgccgc gacggcccgg ccgcagcact gcgactgcgg
     2401 tcgcaggcca atcgggtcgc gcgcgacgtc atggccaagc accggttcgt cgaggtcgag
     2461 acgccgaact tgaccaggtc gaccccagaa ggcgctcgcg acttcctggt gccggtgcgg
     2521 ctccagcctg ggcactggta cgccctgcca cagtcgcctc agctgttcaa gcagctgttg
     2581 atggtggccg gcatggagcg ctacttccag atcgcgcggt gctttcgcga cgaggacttt
     2641 cgtgccgacc ggcaacccga gttcacccag ctcgacatcg agatgagctt catcgatcaa
     2701 gacgacatca tcgccttggg cgaagacatc gtcgccgcgc tgtggcggga gatcctgggc
     2761 tacgagatac cgctgccgat tgcgcgcatg acctatgccg acgcgatgaa ccgctacggc
     2821 tccgacaagc cggatctgcg gttcggcaac gagctcatcg actgcacggc gttctttgca
     2881 tcgactccgt ttcgggtatt ccaggcgcca tacgtcggcg cagtcgtcat gccgcgcggc
     2941 gcatcgcaga cccgcaagga gctcgatgcc tggaccgact gggcgaagtc gcgcggcgcc
     3001 aagggcctgg cctacgtgct cgtcgctgac gacggttcgc tgggtggtcc cgtcgccaag
     3061 aatctgtcgg acgccgagct ggccggtctc gcgggccatg tcgcggcagc acctggcgac
     3121 gctgtcttct tcgccgccgg cgagcgctcg ccgtcacttg agctgcttgg ggcagcgcgt
     3181 cttgagatcg gccggcggtg cgggctgatc gatgagtcgc tct
//
LOCUS       DATCBJ010000183         3219 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_462969, whole genome shotgun sequence.
ACCESSION   DATCBJ010000183 DATCBJ010000000
VERSION     DATCBJ010000183.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 3219)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3219)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3219
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_462969"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..389
                     /locus_tag="VII50_03680"
     CDS             <1..389
                     /locus_tag="VII50_03680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5049976.1"
                     /translation="ESGVDAAEDAAGLRRSRADFYPAERPDLLDRYVADALKCGSLAR
                     TGHRAVGGYAMERVIERLSMPTLLIGATKDPFAYPQLVRLRAALPHAQVAEIAGGMVP
                     LPDGWPAPFAVAVLGFLDAPAGRDGG"
     gene            549..1619
                     /locus_tag="VII50_03685"
     CDS             549..1619
                     /locus_tag="VII50_03685"
                     /inference="COORDINATES: protein motif:HMM:NF024799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HEY5049977.1"
                     /translation="MTTVGAGALAIVCAATLSSTSSGNTPARSADARAATASVLFTSI
                     EQSVPTGYPKPKSGKFRLAYLNPETGNEFLNILGQSMSLETKKLGGSFTQLDAGGDVN
                     TQVTQFNQLIAQKVNGIAVFALDPKSLAPDVARARKAGIHLVTIDLNFTNTKASALGG
                     YESQVLQRRDQAAYLTATYMAKHLAAGSSLGTIDLIVKVPSIVFSIQRDDFWAKKYGL
                     KILGNASNPTDDIAGGEKAATTLINKFSGIKGVMAYNDPSAIGAYTAARSSGVTGLLF
                     GGENGGSDALAAIKAGTETFSLKIDAPGIGKDWAWGLYDLQEGVKVPGTVKAGTPVLV
                     DKANVGSIVSWQNQLKTEYPGS"
     gene            1636..2508
                     /locus_tag="VII50_03690"
     CDS             1636..2508
                     /locus_tag="VII50_03690"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEY5049978.1"
                     /translation="MIETPPLVPEGVTEQIAERVHVIPDQRVEFVPNIGIVVGDRSVL
                     VIETAMGPRNGRRILAEAQRLGEDRKLLLTTTHFHPEHAFGAQPFATAATYICNETQA
                     LELASKGPEYIEMFTGFGPGLAGLLAEVELVAPDATYSGGPARLDLGGVSVELHEIGQ
                     AHTLGDQVVFLPRERILFAGDLVENRFLPIFPDGDASGRRWLEALDRLAALEPAIIVG
                     GHGAVGGVELIAALRDYLTMVRDRVSALQEDGRDLADIEHAVATEVAEPWADWDNQMW
                     IKSAIGSFYSEITG"
     gene            2537..>3219
                     /locus_tag="VII50_03695"
     CDS             2537..>3219
                     /locus_tag="VII50_03695"
                     /inference="COORDINATES: protein motif:HMM:NF013584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HEY5049979.1"
                     /translation="MSLLDAGQISSRQIAQLYLDRIAAVDSQLHAVVSCDPEAVLAAA
                     DAADRARSRGERRALLGLPVTIKDSIDVCGLPCTGGSLGRADHRPATDATVVTRLRDA
                     GALVLAKTNVPEYSSSYETDNLVHGRTNHPLDPARTAGGSSGGEGALAGADASPLGIG
                     TDGGGSLRVPAHYCGVVGLRPTVGRVPETGTWPSTRSSGYMDLFCVGPIARYVEDIAL
                     VLPIISGPD"
BASE COUNT          547 a         1058 c         1090 g          524 t
ORIGIN      
        1 aggagtccgg tgtcgacgcc gcggaggatg ccgctggtct gcggcgctcg cgtgcggact
       61 tttatcccgc cgagcggccg gacctcctgg atcgctacgt ggctgacgcg ctgaaatgcg
      121 gatcgctggc ccggaccggg caccgagcgg tcggcggcta cgcgatggag cgggtgatcg
      181 aacgcctgag catgccgacg ttgctgatcg gggcgaccaa ggatcccttc gcgtatcccc
      241 aactcgtacg gctgcgcgcc gcgctgcctc acgcacaggt cgccgagatc gccggcggca
      301 tggttccgtt gcccgacgga tggccggcgc cgttcgcggt ggccgtcctc ggcttcctcg
      361 acgcgccggc tgggcgtgac ggcggttaga tgtggcgagt ctcggtccga gcggtcgccg
      421 cgtgccgcgc ctcctgcttg accccattct gggccgcgag taaacttgat agttgatcaa
      481 aaagcgttaa acgatccggg aggatgggta tgcgaagttc agtctcaggg gcagcgaagc
      541 ggcgcatcat gaccacggta ggcgccggtg cactcgcgat cgtgtgcgcc gcgacactca
      601 gcagcacctc gagcgggaac acccccgcca ggagcgccga cgcgcgcgcg gcaacggcgt
      661 cggtgctgtt cacgagcatc gaacaatccg tgccgacggg ctatcccaag cccaagtcgg
      721 gcaaattccg gctcgcgtac ctcaaccccg agaccggcaa cgagttcttg aacatcctgg
      781 gacagtcgat gtcgcttgag accaagaagc tcggcggcag cttcacccag ctcgacgccg
      841 gcggcgacgt caatacacag gtcacccagt tcaaccagtt gatcgcccag aaggtcaacg
      901 gcatcgcggt gttcgcgctc gaccccaagt cgctggctcc cgatgtcgcc cgtgcccgca
      961 aggccggaat ccatctggtg accatcgacc tgaacttcac caacaccaag gcgtcagctc
     1021 tcggcggcta cgagagccag gtgttgcagc gtcgcgacca ggcggcgtac ctcaccgcga
     1081 catacatggc caagcacctg gccgcgggct cgtcgctcgg gacgatcgat ttgatcgtga
     1141 aggtaccctc gatcgtcttc agcatccagc gcgacgattt ctgggcgaag aagtacgggc
     1201 tcaagatcct gggcaacgcc tcgaatccga cagatgacat tgcgggcggg gagaaggcgg
     1261 ccaccacgct gatcaacaag ttctcgggga tcaagggcgt gatggcctat aacgacccgt
     1321 cggcgatcgg cgcgtacacg gcagcccgca gctccggggt cactggactg ctgttcggcg
     1381 gtgaaaatgg cggcagcgac gcgctcgcgg cgatcaaggc tggcaccgag acgttcagcc
     1441 ttaagatcga cgctcccggg atcggcaagg actgggcgtg gggtctgtac gacctgcagg
     1501 agggcgtcaa ggttcccgga accgtcaagg ctggcacgcc ggtgctcgtc gacaaggcga
     1561 acgtgggttc gattgtctcc tggcagaacc agctcaagac cgaatacccc ggaagctgac
     1621 ccgaggcgaa catcgatgat cgagacaccg ccactggtcc ccgagggggt caccgagcag
     1681 attgccgagc gcgttcacgt gattccagat cagcgtgtgg agttcgtgcc gaacatcggc
     1741 atcgtcgtcg gggaccgatc ggtgctcgtg atcgagacag caatgggccc acgaaacggg
     1801 cggcggatcc tcgccgaggc gcagcgcctc ggcgaggacc gcaagctgtt gctgacgacg
     1861 acgcattttc atccggagca cgcattcggg gcgcagccgt tcgcgacggc tgcgacctac
     1921 atctgcaacg agacgcaagc cctcgagctg gccagcaagg gcccggagta catcgagatg
     1981 ttcaccggct tcgggccggg cctcgccggg ctgctcgccg aggtcgagct cgtcgcaccg
     2041 gatgccacct acagtggtgg acccgcccgc ctggacctcg gaggcgtctc cgtcgagctt
     2101 cacgagatcg gccaggccca cacgctcggt gaccaagtgg tgttccttcc gcgggagcgc
     2161 atcctgtttg ccggtgacct tgttgagaac cgcttcctgc cgatcttccc cgacggcgac
     2221 gccagcggtc gccgctggct ggaggcgctc gatcgactcg cagccctcga gccggcgatc
     2281 atcgtcggtg gtcatggcgc cgtcgggggg gtcgagttga tcgccgcact ccgcgactac
     2341 ttgacgatgg tgcgcgatcg cgtgtcggcg ctgcaggagg acggccgcga tctcgccgac
     2401 atcgagcacg ccgtcgcgac cgaggtggcc gagccgtggg cggattggga caaccagatg
     2461 tggatcaaga gtgcgatcgg cagcttctac agcgagatca ccggctgacg gtcgccagcc
     2521 gtccgctgtc ggctggatgt cgctgctgga cgccggccag atctcctccc gccaaatcgc
     2581 gcagctctac ctggatcgca tcgcggcggt ggattcgcag ctgcacgccg tcgtgtcgtg
     2641 cgaccccgag gcggtgttgg cggcagccga tgccgccgac cgcgcgcgat cgcgtggcga
     2701 acggcgggcc ttgcttggtc tccctgtgac gatcaaggac tcgatcgacg tgtgcgggct
     2761 gccctgcacc ggcgggtcgc tcgggcgtgc tgaccatcgt cccgccaccg acgcaacggt
     2821 tgtgacccgg ctgcgcgacg ccggtgcgct cgtgctggcc aagaccaacg tgcccgagta
     2881 cagctcctca tatgagacgg acaatcttgt gcacgggcgc accaaccatc cgctggaccc
     2941 cgcgcgcacc gcggggggct cgagcggcgg cgagggcgca ctagccggcg cggacgcctc
     3001 gccgctggga atcggcacgg acggcggggg ctcgctgcgg gtcccagccc actactgcgg
     3061 ggtggtcggc ctgcggccaa ccgtcggacg ggtccccgag accggcacat ggccgagcac
     3121 gcgctcaagc ggctatatgg acctgttctg cgtcggaccg atcgcacgct atgtggagga
     3181 catcgcgctc gtgcttccga ttatctccgg gcctgattg
//
LOCUS       DATCBJ010000184         8893 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_279965, whole genome shotgun sequence.
ACCESSION   DATCBJ010000184 DATCBJ010000000
VERSION     DATCBJ010000184.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 8893)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8893)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8893
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_279965"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            462..1481
                     /locus_tag="VII50_03700"
     CDS             462..1481
                     /locus_tag="VII50_03700"
                     /inference="COORDINATES: protein motif:HMM:NF012742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEY5049980.1"
                     /translation="MTKRPPATLSQVAQRVGVSLTTASKAINGKDRVSELTRARVMAA
                     ARDLSFTPNLVARGLMTGSTSTVGFIIADSMTHRFAAPVMLGAETALGAIDLSMITCD
                     ARGDQARSRELAIMLGARKVDGVLVVGDNNAVTASVTEFIDVPVVYVYGLSDDERDVV
                     HMPDDENGIALAVDHLVATGRRRIAHLTGPRGATAVVERVKGMRARMRREGLRLAGPV
                     MYGDHWSQRWARGATAQLLESHPDVDAVLCGSDQIAAGVVVGLKEAGRRVPDDVAVTG
                     YDNWGVFALETEPPLTTVDMNMEALGAAAAQELFSMIDGGPRQGGIRRHGCGLIVRAS
                     TVIVL"
     gene            complement(1766..2413)
                     /locus_tag="VII50_03705"
     CDS             complement(1766..2413)
                     /locus_tag="VII50_03705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5049981.1"
                     /translation="MTTLPPKVSATAVAPAPATSTSATPTPLPPPAPATSASPSPSAS
                     PTPSASSAASPTGSASPTNLDPCQLVTQDEASSLAGTTFGPGKEESTGGGKECVYGSQ
                     TTNVMDIIVAQAIDAKTAQADYAAAQADAQTTIASKLPPGVHVNLNAANAAGIGDQAV
                     TITGAESLLGQTLSFTGIYVLSGATFFTIGDLVLAKPPPTVSAIEDQARTTLGRI"
     gene            2629..2799
                     /locus_tag="VII50_03710"
     CDS             2629..2799
                     /locus_tag="VII50_03710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5049982.1"
                     /translation="MGTDCGAIDSAHCPNSAIAQIDHLAPLGNLDGWIASQATVSGAD
                     RIGQAIADELST"
     gene            3217..3573
                     /locus_tag="VII50_03715"
     CDS             3217..3573
                     /locus_tag="VII50_03715"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEY5049983.1"
                     /translation="MAVPLYQAQAELFRTLGHPVRIRVLELLQDGPKAVSELLVALGV
                     EASSLSQQLAVLRRAGIVVSTRRGTSVFYELAASDVAELMRAARRFLTEVLADRGDLL
                     AELRSYETASARRRRE"
     gene            3826..4179
                     /locus_tag="VII50_03720"
     CDS             3826..4179
                     /locus_tag="VII50_03720"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEY5049984.1"
                     /translation="MQYRGGVPHNARPVHEVKATLFRVLGHPARVRILELLRDCERSV
                     GVLQGDLDLDSGSTSRHLAALRRIGLVESRRDGTTVFYRVDDERVFDLLAAGRDIITH
                     QLAQQQFLLHDLDLS"
     gene            4176..6122
                     /locus_tag="VII50_03725"
     CDS             4176..6122
                     /locus_tag="VII50_03725"
                     /inference="COORDINATES: protein motif:HMM:NF012581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HEY5049985.1"
                     /translation="MIAVLYWLAGAAIAIGVVVTLLRGPWRRGLGLQAVGAALFIIAG
                     FATLATGHAVGAAFTSQLTPRLGVDRLSGVFLATLGLVAAPALAYSSRYLDGSRRGRL
                     VGALTGVFVLAMVGLLCARDAVTFLGSWELMSLLPAVIILLHRDADAESRRSVFIYIA
                     ITHLGGVGTWVAVLLLAHAGALGTAGVVGPGSGLQIGIALAALVGMGTKAGLVPLHAW
                     LPRAHPMAPAPVSALMSGVMIKLAVYAMIRVFVDWLGVLPTWFGVLVLAVGAVSSVGG
                     VTYALFQHELKRLLALHSIENLGIIVLGLGACLVLRAHDEDVWAALALAAALLHTVNH
                     AVFKALLFLCAGSIERSVGSLELNQLGGLLRRMPWTGGAFFVGAMAIAGLPPLNGFAS
                     EWLTLQALLHVVMSGHVGDGMAGAVALAALGATAALAVFCFVKVVGLVLLGPPRRAHV
                     RDALEVAAPMRAAVTVLAGACIVLGLVPGLLVSRLVAIAPWSGQAPVHAGLYVPGTGS
                     LPTVGIAVVLVAVTVVLTLLRGQHTAHVAPTWVCGQPLVPELNWSSAGFTKPLRLVLE
                     VVLRPRREIEVHTHGGVVTQVAYQGEVPNLIDERIYRPVARWSLAGAAYARRLQSGSL
                     GMYVGYLIGLVLVVLIAGRIGWLG"
     gene            6119..7045
                     /locus_tag="VII50_03730"
     CDS             6119..7045
                     /locus_tag="VII50_03730"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF012373.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit H"
                     /protein_id="HEY5049986.1"
                     /translation="MTASTVASGAVQVIGGLVLSPLLPGLIQHWKARLQGRRGPSPLQ
                     PYRELRRLWNKSTVDVAGTTVVYRLAPAVIGACLVAAVGLVPVNAVGPNLGVGHDVIV
                     LIGLLALARFAAAASTWDVANGFSLMGASRDLTISAFVEATLLLALAVAVMLTGTSNL
                     LGMIARTAGGAVWSSPALPLGALAFGIVIVAETGHQPFDNPDTHLELTMIHETPLLEY
                     AGRDLAYLQWAAAARHWVVLVLAAQIFLPHPRGVWAQLAVLPLVLVVMCGALALVETL
                     QAKMRVLLVPRLLGVAALAALLGVVARGVGGS"
     gene            7042..7653
                     /locus_tag="VII50_03735"
     CDS             7042..7653
                     /locus_tag="VII50_03735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5049987.1"
                     /translation="MTGATGWLLVGLGLGVVVVRRRSIAVGLVTTQALVLAAIALQRA
                     GTGSDVVAGLALAMRAGALGALFFVAVARSRESQPLRAPVPPMVRAGVAVVLILLLTA
                     LVPSFGLTSRPAERAVLALVAAGLACAATRRSTLFQVLGIVLVENGLALAAIESPHAS
                     SLIIELGVTLDLTLIVCVAALFHLRIFSEFGAGDSAALRSLRD"
     gene            7646..>8893
                     /locus_tag="VII50_03740"
     CDS             7646..>8893
                     /locus_tag="VII50_03740"
                     /inference="COORDINATES: protein motif:HMM:NF012581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HEY5049988.1"
                     /translation="MISTQALAVVVVAVPLVTAGLISLAPSKHLTALARTCAVATSAL
                     AVVLVGVAVRDAGHPLAGHWIVVDAAGALLLAVTAVIGLASVLMSASYLHGAHSSFVA
                     EGHRDRVYYVVLFLFWAMLLAVPLAGNLGAAWLLVEATTAASALLVGFSGKAHALEAG
                     WKYLILTSLGLGIALLGIAILAAGVPSGGVSGLAWGRLATFGSGRSTALSAYLLILIG
                     LAAKIGWAPVHNWLPDAHSQAPPPVSALLSAALLPTVLLVAWRTDRALAPLIGASAAQ
                     RVLIGFGLVSLAVAVPFLWRPMAFKRLLAYSSLEHLGVMALGVGFGTPLALAGVALHI
                     VGHAIAKALGFYAATPLLAHEPRAARHAVSGIGRTAPALGATMGLSLGALSGLPPSPL
                     FVSELLILAGGFESGRPWPAAIAA"
BASE COUNT         1235 a         2728 c         3113 g         1817 t
ORIGIN      
        1 cgagatgccg tagggcagcg tggtcagccg gttgcgggcc tgccattgcc cccaccagcc
       61 ggtaggagtc actcgcacgc cggaaagtgg cagcggcaac agccgcgacg cccgtatcgc
      121 gaggcctacg gggccgacga cgtaggtgct cgtggtgtcg accacacgtg ccacgtcagt
      181 caacgcagtt ctccctaggt tctttggtgc atgagctgtt agagcaggcg gcagcgatgc
      241 gtgcctcgcg gtgagaaggc atagaaagag gggccggacg gccctgtggg acgatccacg
      301 aagcaaatga ggaagcgctt cctcaactcg tgtgcacagg ctgtgcctcg cccgacggca
      361 cgtcaagagc accatcctcg gcgttaccaa cttgttacga agcgagcgtc tgtggcgatg
      421 cggtgaatga tcaacgacgc gatggatggc acactcgcgg catgacgaaa cggccgcccg
      481 ccacgctcag tcaggtagcg cagcgggtcg gtgtctcact cacgacggca tcgaaggcca
      541 tcaacggcaa agatcgggtc tcggagttga cgcgcgcccg cgtgatggcc gcggcccgcg
      601 acctctcgtt cacgccaaat ctggtggccc ggggcttgat gaccggcagc acgagcacgg
      661 tcgggttcat catcgccgac agcatgaccc accggtttgc ggcaccggtg atgctcggcg
      721 cggagactgc ccttggcgca atcgacctgt cgatgatcac gtgtgacgcg cgcggcgacc
      781 aggcccgcag tcgtgaactt gccatcatgc tcggagcgcg caaggtcgac ggggttctcg
      841 tcgttggcga caacaacgcc gtcacggcct cagtgaccga attcatcgac gtcccggttg
      901 tgtatgtcta cggcttatcg gacgacgagc gtgacgtggt ccacatgccg gacgacgaaa
      961 acgggatcgc gttagctgtc gaccatctgg tggccaccgg gcggcggcga atcgctcacc
     1021 tcacgggtcc gcgtggcgcc accgccgtcg tcgagcgggt caaaggcatg cgcgcgcgga
     1081 tgcggcgaga aggtctgcgc ctggccggcc cggtgatgta cggcgatcac tggtcgcagc
     1141 gctgggcgcg cggggccacg gctcaactgc ttgagtcgca tcccgacgtc gatgcggtgc
     1201 tatgcgggtc agatcagatt gccgcgggcg tagtggtggg cttgaaggaa gctggccggc
     1261 gagtgcccga cgacgtcgcg gtcaccggat acgacaactg gggcgtgttc gccctggaga
     1321 ccgaaccgcc tctgacaacc gtcgacatga acatggaggc tctcggtgct gccgcagcac
     1381 aagaactctt cagcatgatc gacggcggac cgaggcaggg cggcattcgt cgacatggat
     1441 gcgggctcat cgtccgcgcc tccacggtca tcgtattgtg aatttctcgc ccaatctgtc
     1501 acaaaatcga cggcagccgg atgacctcga cgctccagtt agcccaggcg gccaccttca
     1561 ctcccatact gcgccgtcga cctcctaaat ttggaacatt gtggctgccg tgaaacaaat
     1621 cccgcgcggt caggacacgg tcgatgggct gctggccgaa ggacgcctcg agctgatcac
     1681 ccgggcgggc tgggattgcg tcgccggcgc gacgtgaggt gggcggccgg ggactgttgc
     1741 acatcagctc atcgtgctgc cgttttcaga tccggccgag cgtggtccgg gcttggtcct
     1801 caatcgcaga caccgtgggc ggtggcttgg ctagcaccag gtcgccgatg gtgaagaacg
     1861 tcgcgcccga cagtacgtag atgccggtga aggagagcgt ctgacctagt agcgactcgg
     1921 cgccggtgat cgtgaccgcc tgatcgccga tgcccgcggc gttcgcggca ttgaggttga
     1981 cgtggacccc cggtggaagc ttggacgcga tagtggtctg tgcatcggcc tgcgccgcgg
     2041 cgtaatccgc ctgcgccgtc ttggcgtcga tcgcctgagc gacgatgatg tccatcacgt
     2101 tcgtggtttg agacccataa acgcactcct tgccgccgcc tgtggactcc tctttgcccg
     2161 gcccgaacgt ggtgccggcc agcgacgacg cttcgtcttg ggtgaccagc tggcaggggt
     2221 caaggttggt cggcgacgcg gacccggtcg gtgaggcagc actcgatgcg gacggcgtcg
     2281 gcgaagcgga cggcgatggg gaggccgacg ttgccggcgc tgggggtggc agtggcgtgg
     2341 gcgtagcgga cgtgcttgtt gccggcgcag gcgccaccgc agtggcagac accttcggtg
     2401 ggagcgttgt caccttgctc ttgctcgacg acttgcagga cgcaatgagt aaagcgggag
     2461 taatgacaat aagcatcaga gcggcgcgtc gttgcatttc gggtatctcc gtagccgtag
     2521 cgctgtgcgg gcggtttaga cgattgaaga tgaaatgttg cgcacgcaca tcagtagccg
     2581 aatacgtaga agccgacgcc gcacccgtcg ccgtcctcgt tactccggat ggggaccgac
     2641 tgcggggcga tcgattccgc gcattgccca aacagcgcca tcgcgcagat cgaccatctc
     2701 gcgccgttgg gaaacctaga cggctggatt gcgtcgcagg caaccgtgtc cggagccgat
     2761 cggatcgggc aagcgatcgc cgacgaactg agcacctaac tgcccgacgt gcgcagctcc
     2821 tcctggaccg cccgaggcat gagacccttc atcgcctcaa ggcccaacat ccgcgttctc
     2881 ttctgtcgga ggccgtgccg gcggcattcc cgggatgggt gcctatcaga ctgcgaagtt
     2941 cgcggtggaa ggtttctccg aggtgctcgc gaacgaagtg aagcctttgg tattgacgtc
     3001 gtcatcgtcg aaccaggtgg attccggacg gaccgctgtg gtccgtattg ggccccattt
     3061 taccggccgt acggccccgc tacggccccg ctcgacgggt ttccgggcac ctcctcaaag
     3121 gggtcctgtg gcagcggatc ggcctgcagt ttgaagatct ttggatcttc acatatacaa
     3181 gggactcaag tgccgagcct cggcaagggg tgcgccgtgg ccgttccgct gtatcaagcg
     3241 caggctgaac tgtttcggac cttgggccat ccggtccgga tccgcgtgct cgagttgtta
     3301 caggacgggc cgaaggcggt gagcgagttg cttgtcgcgc ttggcgtcga ggcgagcagc
     3361 ctctcgcaac agttggctgt gttgcgtcgc gcggggatcg tggtgtctac ccggcggggc
     3421 accagcgtgt tctacgagct ggcagcctcc gatgtcgcgg aattgatgcg tgctgcacgc
     3481 cgattcctca ccgaggtact ggccgatcgt ggtgacctgc tggccgagtt gcgctcgtac
     3541 gaaaccgcga gcgctcgtag gcgccgagaa taggacttgg cctgatgttc catggggagc
     3601 ccggccgcgc cgagctgaac gaggaggcag ggaatacgtg tcgaagcgag ccgatcgctc
     3661 cttcgcgagc ccgttgtcgg cacgcccgta gtcgatagct gcggtgtgtg tcaggcgcgg
     3721 gtttcgtccc ggcgccggca ggctcgggac gcgtccacga gcgtggggcg gcgagacggg
     3781 gttgtgtcgg catcggcagc cgtaatactg catatctttg aagatttgca gtatcgtggc
     3841 ggggtgccgc acaatgcccg ccctgtgcat gaggtgaagg cgacgttgtt tcgggtgctg
     3901 ggtcatccgg cgcgggtgcg gattcttgag ttgttgcgtg actgtgagcg ctcggtcggg
     3961 gtcttgcaag gggatctcga tcttgactcc ggcagtacgt cgcggcatct tgcggcgttg
     4021 cggcggatcg gcttggtgga gtcgcgtcgc gacggaacta ctgtctttta ccgtgtcgat
     4081 gatgagcggg tcttcgacct gcttgcggct ggtcgcgaca tcattaccca tcagttagcg
     4141 cagcagcagt ttcttctgca tgacctcgat ctctcgtgat cgccgtcctg tattggcttg
     4201 ccggcgcagc catcgcgata ggtgtcgtcg tgacgctcct gcgtgggccg tggcgacgtg
     4261 gtcttgggtt gcaggctgtc ggcgccgcac tgttcataat cgcggggttc gcgactctgg
     4321 cgactggaca tgcggttggc gcggcgttca cgagtcagct cactccgcga ctcggtgtcg
     4381 atcgcctctc cggggtgttc ttagcaaccc tcggtctggt tgcggcaccg gcgttggcgt
     4441 attcctcccg gtacctcgac ggctcccgtc gcgggcgact ggtcggtgcg ttgaccgggg
     4501 tgttcgtgct ggcgatggtg gggctgctgt gtgcgcgcga tgccgtcacg ttcctcggca
     4561 gttgggagct catgagcttg ctgccagcgg tgatcatcct cctccaccgc gacgctgacg
     4621 cggagtcgcg tcgtagcgtc ttcatctaca tcgcgatcac ccacctcggc ggggtcggta
     4681 cgtgggtggc ggtgctgttg ctcgcacatg cgggggcgtt aggaacggcc ggcgtcgtcg
     4741 ggccagggtc cggcctgcag atcggcatcg cgttggcggc gttggtcggc atgggcacca
     4801 aggccggcct cgtgccgttg cacgcgtggt taccccgggc gcacccgatg gcgccggcgc
     4861 cggtttcggc attgatgagc ggcgtcatga tcaagcttgc ggtgtacgcg atgatccgcg
     4921 ttttcgtcga ttggcttggt gtgctgccga cctggttcgg cgtcctcgtc ctcgccgtcg
     4981 gtgcagtgtc ctccgtcggt ggcgtcacct atgccttgtt ccagcacgag ttgaaacggc
     5041 tgttggcgct gcactcgatc gagaacctcg gcatcatcgt gttgggtctt ggtgcttgcc
     5101 ttgtgcttcg tgcgcacgat gaggatgtct gggctgcgct tgcgttggct gcggcgcttc
     5161 tccataccgt caaccatgcg gtgttcaagg cgttgctgtt cttatgtgcg ggctcgattg
     5221 aacgatcggt cggctcactc gaactcaacc agctcggtgg tctgttgcgg cggatgccgt
     5281 ggaccggcgg agcgtttttc gtcggggcga tggcgatcgc agggttgccg ccgctgaacg
     5341 gttttgcttc ggagtggctg acgttgcagg cgctgttgca tgtggtgatg tcgggtcacg
     5401 ttggtgatgg tatggcgggt gcggtcgcgc tggctgcgct gggggcgaca gcagcgctcg
     5461 ccgtgttttg tttcgtcaaa gtggttggtc tcgtgctgct cgggccgccg cggcgcgccc
     5521 atgtccgcga tgcgctcgag gtcgcggcgc cgatgcgcgc agcggttact gtgctcgccg
     5581 gggcgtgcat cgtgttgggg ctcgtgccgg gtctattggt gagccggctc gttgcgatcg
     5641 ctccttggtc ggggcaggcg ccagtccatg ccggtcttta cgtgcccggc acagggtcgt
     5701 tgccgacggt cgggatcgcg gtggtcctcg tcgcggtgac cgtggtgctg actctgctgc
     5761 gtgggcaaca taccgcccat gttgccccga cctgggtgtg cgggcagcca ttagtgcccg
     5821 agctcaactg gtcgagcgcg ggcttcacca agccgttgcg gctggtgctc gaggtcgtgt
     5881 tacgaccgcg ccgggagatt gaggtacaca cgcacggcgg cgtcgtcacc caggtcgcct
     5941 accagggcga ggtgcccaac ctgatcgacg agcggatcta tcgtccggtt gcgcggtggt
     6001 cgctcgccgg tgctgcgtat gcgcgccgct tgcagagcgg aagcctcggg atgtatgtcg
     6061 gctacctcat cggcctggtg ctcgtcgtcc tgatcgccgg ccggatcggc tggctcggat
     6121 gacggcgtct acggtcgcca gcggcgccgt ccaggtcatc ggtgggctcg tgttgtcgcc
     6181 gctattgcct ggcttgatcc agcattggaa agcgcgactg caaggtcggc gcggtccatc
     6241 gccgctgcag ccctaccgcg agcttcgccg gctatggaac aagagcaccg tcgacgtggc
     6301 agggacgacc gtggtctacc gcctcgcgcc ggcggtgatc ggcgcctgcc tcgtcgccgc
     6361 ggtcgggctc gtcccggtga acgcggtcgg acccaacctc ggcgtcggac acgacgtcat
     6421 cgtcttgatc ggcttgttag ccctcgcccg tttcgccgcg gctgcgagca cgtgggatgt
     6481 tgccaacgga ttctcactga tgggcgcgag ccgcgatctg acgatttccg cgttcgttga
     6541 agcgacgttg ctgctagcgc tggccgtcgc cgtgatgctg acgggcacgt cgaacctgct
     6601 cgggatgatc gcccgcaccg ccggcggtgc ggtctggtcg agccctgcgc tgccgttggg
     6661 tgcgttggcg ttcggaatag tgatcgtcgc ggagaccggc catcagccgt tcgacaaccc
     6721 cgacacgcac ctcgagctga cgatgattca cgagacgccg ctgctcgaat atgccggtcg
     6781 cgatctcgcc tatttgcagt gggcggcggc tgcccgacat tgggttgtcc tggtgctcgc
     6841 cgcgcagatc ttcctgccgc acccgcgcgg tgtctgggcg cagcttgcgg tgttgccgtt
     6901 ggtgcttgtg gtcatgtgcg gtgcgctcgc gcttgtcgag acgttgcagg ccaagatgcg
     6961 tgtgctactg gtacctcgcc tgctcggtgt tgcggcgttg gctgcgctgc tcggtgttgt
     7021 cgctcgtggg gtcggtgggt cgtgacgggt gccaccggat ggctgctggt cggccttggg
     7081 ctcggtgtcg tcgtggtgcg gcgtcgatcg atcgcggtcg gtctcgtgac gacgcaggcg
     7141 ctggtgctcg cggccatcgc gctgcagcgg gccggcacgg gaagcgacgt cgtcgctggg
     7201 ctggcgctcg ccatgcgcgc gggcgcactc ggtgccctgt tcttcgtcgc ggtggcccgg
     7261 agccgggagt cgcaaccgct gcgagcgcct gtccctccga tggtgcgcgc cggcgtcgca
     7321 gtggtgttga tcctgctgtt gacggcgctg gtcccctcct tcggcctcac ttctcgcccg
     7381 gcggaacgtg cggttcttgc cctcgtcgcg gctggtctgg cgtgtgctgc gacgcggcga
     7441 tccacgctgt ttcaggtact cggcatcgtg ctcgtcgaga acgggctggc gctggcggcg
     7501 atcgagtcac cgcacgcctc gtcgttgatc atcgaactcg gcgtgacgct cgacctcacc
     7561 ttgatcgtgt gcgtcgcggc gctgttccac ttgcgcatct tcagcgaatt cggcgcgggc
     7621 gacagcgctg cgttgcggag tttgcgtgat tagcacccaa gcgctcgcgg tcgtcgtcgt
     7681 cgcggtaccg ctcgtgactg ccgggctcat tagtcttgcc ccaagcaaac atctcaccgc
     7741 gctggcgcgc acctgtgccg tggcaacctc agcactcgct gtggtgcttg ttggtgttgc
     7801 cgtgagggac gccggccatc cgctggccgg tcactggatc gtggtcgacg ccgcgggtgc
     7861 gttgctgctc gcagtgactg ccgtcatcgg gctagcgagc gtgctgatgt cggcgtccta
     7921 tctgcatggc gcgcacagct cgtttgtcgc cgagggccat cgtgaccgcg tctactacgt
     7981 tgtgctgttt cttttctggg cgatgctgct cgcggtgcca ctggccggca acctcggcgc
     8041 ggcctggctg ctcgtcgagg cgacgaccgc cgcgtccgcg ctgctggtgg gtttcagcgg
     8101 caaagcacac gcactggagg cgggctggaa gtacctgatc ctcacctcgc ttggtctggg
     8161 tatcgcgctg ctcggtatcg cgatcctcgc cgctggtgtg ccgagcggtg gggtcagcgg
     8221 actggcatgg ggacggctgg cgacgttcgg atcggggcgc tcgaccgcgc tgtcggcata
     8281 cctgttgata ctgatcggcc tggccgcgaa gatcggatgg gcgccggtgc acaactggct
     8341 gcccgacgcg cactcccaag ccccgccgcc ggtgtcggcg ctgctatcgg ccgcactgct
     8401 accgaccgtg ttgctcgtcg cgtggcgcac cgaccgtgcg ctcgcgccgc tgatcggagc
     8461 atcggcagcg cagcgtgtgc tgatcggatt cgggctcgtg tcactcgcgg tcgcggtccc
     8521 gttcttgtgg cggccgatgg catttaagag gctgcttgcc tactccagcc ttgaacatct
     8581 cggggtgatg gccctcggcg ttggcttcgg cacgccgctg gcactcgcag gcgtcgccct
     8641 gcacatcgtg ggacacgcca tcgccaaagc gctcggcttc tacgcagcaa cgccgctgtt
     8701 ggcgcacgag ccgcgcgcgg cccggcacgc ggtcagcggc atcggacgca ctgcacctgc
     8761 gctcggtgca acgatgggct tgtcgctcgg cgcattgtca gggctgccgc cgtccccctt
     8821 gttcgtcagc gaactactga tcctcgcggg cggtttcgag tccggccgtc cgtggccggc
     8881 tgctatcgcc gcg
//
LOCUS       DATCBJ010000185         4434 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_156006, whole genome shotgun sequence.
ACCESSION   DATCBJ010000185 DATCBJ010000000
VERSION     DATCBJ010000185.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 4434)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4434)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4434
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_156006"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1263)
                     /locus_tag="VII50_03745"
     CDS             complement(<1..1263)
                     /locus_tag="VII50_03745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5049989.1"
                     /translation="MRHRRPLRALTALVFGTASFAVVGLSAAAPAGATPGPVTLDSGP
                     SGQPNDVELFGASTAGILYSVTDIAGGGIEHFFMQPTGGSSTPVPAGIYPSTVAAAVV
                     GPMIGGFDGVSTFTYTTITGSASGTSTVPAGATFQTTSADGYLYSAVPNGVTAPHIYD
                     VNVTAHTTTDLGAVPGNPAPFTLIASTTGVLVETYATPPAVQPESLYYLTYTAPGQFT
                     EFTSSVAVQGVPVLGANAAAWIENPTLSTDESSFVPADTTVVRIGLDGSNEVRTAIPD
                     AVGVALTSTLTGVTTQPPPNIPQTFATMPAAGGALTPYPIQLPEAAMAFGNSFIANQN
                     GTPTTAGLYTMPSAAGPATQLIAAGAQTLRATALALSPGRAAWVDNGVPAGTWSRPLS
                     TTGLTITPGTPSVLSSISDPSDRTPISSS"
     gene            complement(1399..1629)
                     /locus_tag="VII50_03750"
     CDS             complement(1399..1629)
                     /locus_tag="VII50_03750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5049990.1"
                     /translation="MVAPRVAGMLGGMSWESGPAADSRPVGPKCFSCTNTMHKVADQV
                     HTPFAYRFFTSGTSPGRQSPTPGCVPSLASSS"
     gene            complement(1923..2075)
                     /locus_tag="VII50_03755"
     CDS             complement(1923..2075)
                     /locus_tag="VII50_03755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5049991.1"
                     /translation="MDLPDGLQLREERFIDGEPLSSDRELAVGLGKEIGKIGRCRGCR
                     RKLSLR"
     gene            2263..2946
                     /locus_tag="VII50_03760"
     CDS             2263..2946
                     /locus_tag="VII50_03760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5049992.1"
                     /translation="MTDRPDVWSTEPTEPIGAPQGALSDAFVAEQTRMRSTRAVAIRG
                     LQQRRWRRRVLPPLAVTSVALLGTGAAGAWVNAALTVKSAAPAAPDASGQLAALEQLR
                     QQLSADQQALAAITVALRPSSPQTASSSAVTATTSAPAPGPTVLPATRPKSTSGSAAL
                     KPATVPRLAGPTTVAAKPSSPAAVAVIPVAKPTVANPAPAPAAPPTQAPAPVPPPVHA
                     TTGASGAKP"
     gene            3002..3910
                     /locus_tag="VII50_03765"
     CDS             3002..3910
                     /locus_tag="VII50_03765"
                     /inference="COORDINATES: protein motif:HMM:NF014477.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HEY5049993.1"
                     /translation="MATDVAITAVGGDHARLRSAVDQALAVFRDVESQCSRFDPHSGL
                     MKANAGAAEWTLVPSRCFEALAAAASAYLRTRGRFDPRVHDDLCRLGYARSLAFEAGD
                     VVLRTPSARPRRELPEWKPRFRPRLGLVHLGGYRVDLGGIGKGLAVRWSAETIREHTD
                     NFLVEAGGDCYAAGYAPDGGPWRIGVEDPLNLAQPIAVLAVSDRAVATSSVRIRRWTA
                     GGSEVHHLIDPRTGLPGGAGLCAVTVVGADVADAEVDAKALFLEGQLAISDAVRSDLT
                     AACWVTTDGAYEFSKAFEPYIQWRRA"
     gene            3907..>4434
                     /locus_tag="VII50_03770"
     CDS             3907..>4434
                     /locus_tag="VII50_03770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5049994.1"
                     /translation="MSDQHPRRFDWIAASVAFCLSVPSGYLVMRFALPVVHNRYFPWI
                     VGRACGLAAYVALVILVVLGMWLRHPWRHHFRWPHAEEQFRLHAAMGVAVVALVGAHI
                     VALVLDSYAGVRWLAVVVPWKASYRPTAVSLGVFGLYGLLAVSLTARIGGRAIGRQWR
                     RVHMLAFPTFALVWFH"
BASE COUNT          723 a         1445 c         1489 g          777 t
ORIGIN      
        1 ggacgacgaa atcggcgtcc tgtccgatgg atcggatatc gaggagagaa cgctcggagt
       61 gcccggcgtg atcgtcaggc ccgtcgtaga caacggccga ctccacgtgc cggctgggac
      121 tccgttgtcg acccaagccg cgcggccagg tgacagtgcg agcgcggttg cccgcaacgt
      181 ctgcgcaccg gcggcgatga gttgggtcgc ggggccagcg gccgacggca tcgtgtacag
      241 ccctgccgtg gtcggggtgc cgttctgatt cgcgatgaag gaattgccga acgccatggc
      301 tgcctcaggc agctgaatcg gatagggcgt gagcgcgccg ccagcagccg gcattgtcgc
      361 gaaagtttgg gggatgttcg gcggaggctg ggtggtcacg ccggttagcg tcgaagtgag
      421 ggcgacgcca actgcatcgg ggatggccgt gcggacttca tttgagccgt cgaggccgat
      481 gcgtacgact gtcgtgtcgg ccggcacgaa ggacgactcg tctgtgctaa gtgtcgggtt
      541 ctctatccaa gccgctgcgt tggctcccag cacgggaacg ccttgcaccg ccacggacga
      601 tgtgaactcg gtgaactggc ccggcgccgt gtaggtgagg tagtacaggg actcgggctg
      661 aacagctggt ggcgttgcat acgtctcaac gagcacgcca gttgtcgacg cgataagggt
      721 gaatggcgct gggttgccgg gaaccgcccc tagatcggtc gtcgtgtggg cggtcacatt
      781 gacgtcgtag atgtgggggg ctgtcacgcc gttcggcact gcagagtaca agtacccgtc
      841 ggcgctcgtg gtctggaacg tggcgcccgc cggaacggtc gacgtgccac tggcagagcc
      901 ggtgatcgtt gtgtacgtga atgtcgagac cccgtcgaag ccgccgatca tcgggccgac
      961 gactgcggct gccaccgtgg acgggtagat gcccgccggc actggagtgc tggagccgcc
     1021 ggtcggttgc atgaagaagt gctcgatgcc acctccggcg atgtcggtga ccgagtacag
     1081 gatgccagcg gtgctagccc cgaacagctc gacgtcattc ggctgtcccg acggcccaga
     1141 gtctaatgtc actggaccgg gagtggcgcc ggccggagcg gccgcactga gaccgactac
     1201 ggcaaagctt gcggtgccga agacgagcgc ggtcaaggcg cgcaacggcc tgcggtgtcg
     1261 catgaaaatc tcccccgtat gtgactcaag ccagctcagc agtcaaacca actctaagtg
     1321 ccgcccttca acgggggcag cggattcagc gaaccgttcg gttgacgcgc ccagctgatt
     1381 agcccaacgt gagtaccgtc atgacgagct ggcgagtgac ggtacgcagc ctggcgtcgg
     1441 cgactgcctt cccggcgacg tccccgatgt gaagaagcgg tatgcgaacg gcgtgtgcac
     1501 ctgatccgcg accttgtgca tcgtgttggt gcaggagaag catttcggcc ccaccggcct
     1561 tgagtccgcg gccggcccgg actcccagct catcccgccc agcatcccgg ccacgcgtgg
     1621 agcgaccatg actccatcct cacacgaccg cttcgtgagc gaactctcat ctgcacctta
     1681 acgtttggtc accgtctcct gccgatgctg catgtggcaa gtgcgggagg agtgagatga
     1741 agagtgcaac tcgagtactc gcgatgatcg ccatgggcgg cggcatggca ggcggcgcaa
     1801 tggctgtttc gcaggtgccg gcaccacagt ccgctacgac ggtcgcagtc tctggccccg
     1861 gcgcgaccga ggcggtgcag cagctggccg atgagagcgc ccaactccgc gtggcgatcg
     1921 catcagcgca gactcaactt gcggcggcac cctcggcatc ttccgatctt gccgatctcc
     1981 tttccaaggc cgacagccaa ctcgcgatcg ctcgacagcg gctcgccgtc gatgaagcgc
     2041 tcctcgcgaa gttgcaagcc gtcgggcagg tccaacatgc gacgcccgta gtgcctacgc
     2101 cgaagctagc gccgacagac gtgaatgggc ggccagcgcc gtctgacgcg cggagcacat
     2161 cgcctgtggc ccagcaaccc gctgaaccga cggccacaac cgccccacca acgcgtcaac
     2221 gcacgagctc accatcagcg cgaccgacga atcgaggtgg cgatgactga ccgaccggat
     2281 gtttggagca ctgagccgac ggagccgatt ggcgcaccgc aaggtgccct gtccgacgcg
     2341 ttcgttgctg agcaaactcg gatgcgcagc actcgggcgg ttgccattcg tggcctgcag
     2401 caacgccgtt ggcgtcgtcg cgtactaccg ccgctcgccg tgacgtcggt cgcgctactc
     2461 ggaacggggg cggccggcgc atgggtcaac gccgcgttga cggtgaagag tgcagcaccg
     2521 gctgcccctg atgcgagcgg tcagctagcc gccctcgagc aactgcggca gcagctgtca
     2581 gccgatcaac aggcactcgc agctatcacg gtcgcattgc gcccatcgag cccgcagacc
     2641 gcgagttcgt ccgctgtcac agctactacg agcgcacctg cccctggccc aaccgtgctg
     2701 ccagcgactc gtcctaagtc gacgagcggt agtgcggcgc tgaaacccgc caccgtccca
     2761 cgcctcgctg gcccgacaac ggtggcagcc aagccgtcca gccccgcggc ggttgcagtg
     2821 attccggttg cgaagccgac ggtcgccaac cccgcgcccg cgcccgccgc tccgccgacc
     2881 caggccccgg cgccggtccc tccgcccgtg catgccacga cgggggcatc aggtgcaaag
     2941 ccgtaaccgt tccgggacta cgcacccgtc ggtctgtcag gtgatcgggc gcactcgcgc
     3001 gatggcgacg gatgtcgcga tcacagccgt cgggggggat catgcgcgcc tccggtcggc
     3061 ggttgaccaa gcgctcgccg tcttccgcga tgtcgaatcg caatgcagtc gattcgatcc
     3121 ccatagcggt ttgatgaagg ccaacgcagg ggctgccgag tggaccctcg tcccgtcacg
     3181 ttgcttcgag gccctggccg cagctgcgtc cgcctacctg cgcacccgcg gtcgattcga
     3241 ccctcgcgta catgacgact tgtgccgtct cggatacgca cgaagtctcg cgttcgaagc
     3301 cggcgatgtt gttcttcgta cgccgtcagc gcggccccgg cgggagctac cggagtggaa
     3361 accccgattc cgcccacgac ttggcttggt gcacctgggc gggtaccggg tcgacctcgg
     3421 tggcatcggc aaaggcttgg ccgttcgctg gtcggccgaa accattcgcg aacataccga
     3481 caacttcctc gtggaagccg gtggcgattg ctacgcggcg ggatacgcgc cggacggcgg
     3541 accgtggcgg atcggggtcg aggatccact gaatttggct cagcccatcg ccgtgctggc
     3601 cgtctcagat cgcgcagtgg cgacgtcatc ggtgcgcatc cggcgctgga cggcgggcgg
     3661 ttccgaggtg catcacctca tcgatccacg cacgggtctt ccgggtggcg ccggtttgtg
     3721 tgcagtgacc gtcgtgggcg ccgatgtggc agacgcggag gtcgacgcca aggcgctctt
     3781 cctcgaaggg cagctcgcga tcagcgacgc cgtccgctcg gacttgaccg cagcgtgctg
     3841 ggtcacgacc gacggcgcgt acgagttcag caaggccttc gagccataca tccagtggcg
     3901 gcgcgcgtga gtgatcagca cccgcgccgg ttcgactgga tcgcggcaag cgtcgcgttc
     3961 tgcctcagtg ttccgagtgg ctatctcgtg atgcggttcg cgctgccagt ggttcacaac
     4021 cggtacttcc cttggattgt cggccgcgcg tgcggattgg ccgcttacgt cgccctcgtc
     4081 atcctcgtgg tgttgggaat gtggctgcgc cacccgtggc gccaccattt tcggtggccg
     4141 catgcggagg aacagttcag actgcacgcg gcaatgggag tcgccgttgt tgcgctcgtc
     4201 ggcgcgcaca tcgtggccct ggttctcgac agctacgcag gggtccggtg gctcgccgtc
     4261 gtcgtgccgt ggaaggcctc gtaccgcccg accgcagttt cgctcggcgt cttcgggttg
     4321 tacggcctgc tcgccgtctc tcttactgcc cgaattggcg gccgtgcaat cgggcggcag
     4381 tggcggcgtg tgcacatgct tgcgtttccg acattcgcgc tcgtctggtt ccac
//
LOCUS       DATCBJ010000186         4226 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_454958, whole genome shotgun sequence.
ACCESSION   DATCBJ010000186 DATCBJ010000000
VERSION     DATCBJ010000186.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 4226)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4226)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4226
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_454958"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..273)
                     /locus_tag="VII50_03775"
     CDS             complement(<1..273)
                     /locus_tag="VII50_03775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5049995.1"
                     /translation="MYSLVNAPTVGFDLARLPTGAAVATILLEALALQGTVGEPGLDL
                     SGFDGAQVADPQRAAAWLEVSALTPPRRLDDALSAVGQLLDEEAFAM"
     gene            complement(409..843)
                     /locus_tag="VII50_03780"
     CDS             complement(409..843)
                     /locus_tag="VII50_03780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HEY5049996.1"
                     /translation="MEMRQFATFTIDALLFGVEVHKVQEVIRYQEMTAVPLAGRGAAG
                     LINLRGEVVTAVDLRMRLGLPPRAAGVLPMNVVVRVDDEPVSLLVDAIGDVAHVSSEQ
                     FEEPPETMTGPGATLVTGAYKLETTLLLVLDVARAADIGIAV"
     gene            complement(845..3496)
                     /locus_tag="VII50_03785"
     CDS             complement(845..3496)
                     /locus_tag="VII50_03785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HEY5049997.1"
                     /translation="MDEFDEIVREFLVESYENLDQLDRDLVALEQEPDAHPLLQSIFR
                     TLHTIKGTSGFLAFGKLEVLTHAGEGLLARLRDGGLRLTQERTTGLLELVDAVRGILA
                     SIEATGTEDDRDYTDLVARLTALQTALPEIELATASTASRSRSTRARSQATPSSAPTP
                     STSTASSEPSAPAPKTAKAAARPRRMATDARPTAPTDARPRAAPANGATEQVAKHDIK
                     AARAARRANPSAAAAPASPPANVPLLGELLQQQADIDAEDVAVAMLEQQLGDDRPLGE
                     ILVDHGQATPDQVREALALQAVAQADARAGGSVSDSTLRVDVNLLDTLMRLVGELVLT
                     RNQIVATAAARADTGMQRSSQRLNLLVSELQAGVMKTRMQPIDTVWNKLPRLVRDVSV
                     ACGREVRLVLEGRDTELDKTILEAIKDPLTHLVRNAIDHGIEAPEARIAAGKPVEGTL
                     TMRAFHEGGQVNIEISDDGAGIDPARIATKAIERGLATVEQLRAMPPREIVNLIFVPG
                     FSTATKVTNVSGRGVGMDVVRTNVEKIGGTVDISTEPGLGTTLKIKIPLTLAIIPALT
                     VECAGERYAVPQVSLDELLCLEGDQARAGVEYLSGAPVFRLRGTLIPLVWLDHVLDLT
                     DAPHVDESVYILVLQADGRRFGLVVDRVLNTEEIVVKPLSRQLKGIGTYAGATILGDG
                     RVALILDVRALARRAHVVRESDSAMERERHLAAPAVIEGPEPLLVVAVGDRRLGVPLS
                     MVTRLEEISLDRVEHVGQREVVQYRGQLLPLGRLSTMLGEQDMTDAGSQLKVIVYTQG
                     RRSVGLVVDAVVDIATEAPASRGDVAAFGVSGVVVVQELVTELLDMEQAIMATDPRFF
                     DAVAAAGGADVSPYEDYAEDTYVGMGS"
     gene            complement(3612..>4226)
                     /locus_tag="VII50_03790"
     CDS             complement(3612..>4226)
                     /locus_tag="VII50_03790"
                     /inference="COORDINATES: protein motif:HMM:NF024117.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HEY5049998.1"
                     /translation="QSGGWLARGRVLVRRYVATLDPAREPLGVERTIAARTQRLALSG
                     RVDRLDDRTGELVIVDYKTGRRPLTVDDTRGSMALALYAIAAAATLRRRCVRVELHHL
                     PTGDVHAFEHTDESLQRQLRRAEQIADEAEAATTAFKASAAAPGGTVAPEGATDVFAP
                     RPGAQCGWCDLRAHCPEGAAAAPPQLPWAGLGDVDAAPQAVAGP"
BASE COUNT          699 a         1427 c         1425 g          675 t
ORIGIN      
        1 catcgcgaac gcttcctcgt cgaggagttg cccgacagcc gacaacgcgt cgtccaggcg
       61 tcgcggcggg gtcagtgccg acacctcgag ccacgccgcc gcacgctgcg gatcggcgac
      121 ctgcgcacca tcgaagccgg acaagtcgag cccaggctcc ccgaccgtgc cctgaagagc
      181 cagcgcctcg agcaagatgg tggcgactgc ggcgccggtc ggtaggcgcg cgaggtcgaa
      241 gccgacagtc ggcgcgttca ccagcgagta caccgaagcc ctccggaacc atcaacctca
      301 gcaggctcga gggataggtc acagatgacc tgcccccgca gctcaggtac ctgatggtcc
      361 cactcaccgg ggtccgcccg agacggccga ccgggcgaac ccgtgaggtc agacggcgat
      421 gccgatatcg gccgcgcggg caacgtcgag caccaggagc aacgtggtct cgagcttgta
      481 agcgccggtc accagcgtcg cgcccggacc cgtcatggtc tcgggcggct cctcgaactg
      541 ctccgaggac acgtgcgcga cgtccccgat cgcatcgaca agcaagctca ccggctcatc
      601 gtcgacccgc accacgacgt tcatgggcag gacgccggca gcgcggggcg gcagcccgag
      661 ccgcatgcgt agatcgacgg cggtgacgac ttcaccgcgc agattgatca ggcctgcggc
      721 gccgcggcca gcgagcggaa cggcggtcat ttcctggtaa cggatgactt cttgcacctt
      781 gtgcacctct acgccgaaga gcaacgcatc gatggtgaag gtggcgaact gacgcatctc
      841 catatcaaga ccccatgcca acgtaggtgt cttccgcgta gtcctcgtac ggcgacacgt
      901 ccgcgccgcc tgcggctgct accgcatcga agaagcgcgg gtcggtggcc atgatcgcct
      961 gctccatatc gaggagctcg gtcaccaatt cttggacaac gacgaccccg gacacaccga
     1021 aagcggcaac atctccccgg gacgccggag cttcggtcgc gatgtcgacc acagcgtcaa
     1081 cgacgagacc cacactgcgc cgaccttgcg tgtacacgat caccttcagt tgcgatccgg
     1141 catcggtcat gtcctgctcg ccgagcatgg tggacaagcg cccgagcggc agcagctggc
     1201 cgcggtactg cacgacctcg cgctgaccga cgtgctcgac gcgatccagc gagatctctt
     1261 caagccttgt caccatcgac aacgggacgc cgagccgacg gtcgccgacc gcgacgacca
     1321 gcagcggttc cggaccctcg atgacggcgg gggccgcgag gtggcgttcg cgctccatgg
     1381 ccgaatcgct ctcgcgcaca acgtgggcgc gccgtgccaa tgcgcggacg tcgaggatga
     1441 gcgccacgcg tccgtcgcca agaatcgtcg cgccggcata cgtaccgatt cccttgagct
     1501 gacgcgacaa cggcttgacg acgatttcct cggtgttaag gacacggtcg acgacgaggc
     1561 cgaaccggcg cccgtcagcc tggaggacaa ggatgtagac agactcatcg acgtgcggcg
     1621 cgtccgtcag gtcgagaacg tgatcgagcc agacgagcgg gatcagggtg ccgcgcagcc
     1681 ggaagaccgg cgcacccgac aggtactcga caccggccct ggcttggtct ccttcgagac
     1741 acaacaactc gtcgagactg acctgcggaa ccgcgtatcg ctcacccgca cactcgaccg
     1801 tcagcgccgg aatgatcgct agcgtcaacg gaatcttgat tttcaacgtg gtgccgagac
     1861 ctggctcggt ggagatgtcg actgtaccgc caatcttctc cacgttggtg cggacgacgt
     1921 ccatcccgac gccgcgaccg gacacgtttg tgaccttggt tgcggtggag aaacccggca
     1981 cgaagatgag attgacgatc tcgcggggcg gcatcgcgcg cagctgctca accgtcgcca
     2041 ggccgcgctc gatggccttc gtcgcgatcc tcgccggatc gatgcctgcg ccgtcgtcgc
     2101 tgatttcgat attgacctgg ccaccctcgt ggaaggcgcg catcgttaac gtgccttcaa
     2161 ccggcttgcc tgcggcgatg cgggcctcag gagcttcgat gccgtggtcg atcgcgtttc
     2221 gcacaaggtg ggtcagcgga tccttgatgg cctcgaggat cgtcttgtcc agttcggtgt
     2281 ctcggccctc aaggacgagc cggacctcgc gaccgcaggc caccgacaca tcgcgcacca
     2341 ggcgcggcag cttgttccag acagtgtcga tcggctgcat ccgggtcttc atgaccccgg
     2401 cctggagctc gctcaccagc aggttcaatc gctgtgacga acgctgcatg ccggtatccg
     2461 cacgggcggc ggcggtcgcg acgatctgat tgcgggtgag aaccagttca ccgacgagac
     2521 gcataagggt gtcgagcaag ttcacgtcga cgcgcagcgt ggagtcgctg accgacccac
     2581 cggcgcgggc gtccgcctgt gcgacggcct gaagtgcgag tgcttcgcgc acctggtcgg
     2641 gcgtggcctg gccatggtcg acgaggatct cgccgagcgg tcggtcgtcg ccaagttgct
     2701 gctcgagcat cgcgaccgca acgtcctcgg cgtcgatgtc ggcctgttgc tgaagcagtt
     2761 caccgaggag cggaacattc gccggcgggc tcgcaggcgc agcggcggca ctcgggttgg
     2821 ctctacgcgc cgcacgagcc gccttgatgt cgtgcttggc aacctgctcg gtcgctccgt
     2881 tcgccggggc ggcacggggc cgggcgtcgg tcggcgcagt gggccgggcg tcggtcgcca
     2941 tacgccgagg ccgagcggcc gccttcgccg tcttcggtgc gggcgcgctt ggctccgaac
     3001 tggcagtcga cgtgctcggt gtcggggcgc ttgatggggt ggcctgggaa cgcgccctgg
     3061 tcgacctgct gcgggacgcc gtgcttgcgg tcgccagctc gatctccggc agcgccgtct
     3121 ggagggcagt gagccgggcc accagatccg tgtaatcacg gtcgtcttcg gtaccggtcg
     3181 cctcgatcga agcgaggatg ccgcgtaccg cgtcgacgag ttccaggagg ccggtcgtgc
     3241 gctcctgggt caaccgcagc ccgccgtcgc gtaggcgcgc gagcaggccc tcgccggcgt
     3301 gcgtgagcac ctcgagcttg ccgaacgcga ggaaaccgct cgtgcctttg atcgtgtgga
     3361 gggtgcggaa gatgctctga agcagcgggt gggcatcagg ttcctgctcg agcgcgacca
     3421 agtcacggtc gagttggtcg aggttctcgt agctttccac gagaaactcg cgcacgatct
     3481 cgtcgaactc gtccatctac gctcctctgc tcgccgaggg tgtcgcgtcc cgcgacggcc
     3541 gccgggcacg acgatctgtg catcggcgct tctgcggaac aactgagcct caaatcgcga
     3601 ctgagctcgg gtcagggccc agccacggcc tgcggagcgg cgtcgacgtc tcccagaccg
     3661 gcccatggca gctggggcgg tgccgctgcg gcaccctccg ggcaatgcgc ccgcaggtcg
     3721 caccagccac attgcgcgcc gggccgtggc gcgaacacgt cggtagcgcc ttctggagca
     3781 acagtgccac cgggagcggc ggcgctcgcc ttgaaggcgg tcgtggcggc ttccgcctcg
     3841 tccgcgatct gctcggccct gcggagctgg cgttgcaggg actcgtcggt gtgctcgaat
     3901 gcatgcacgt cgcctgtggg caggtgatgc aactcgactc gaacgcagcg ccgccgcagc
     3961 gtcgccgcgg ccgcgatcgc atacagggcg agcgccatcg agccgcgggt gtcgtcgacc
     4021 gtcagcgggc ggcggccggt cttgtagtcg acgatgacca gctcgccggt gcggtcatcg
     4081 agccggtcga cgcgcccgga caacgcgagc cgctgggtgc gggcggcgat cgtgcgctcg
     4141 acgcccaacg gctcgcgagc aggatcgagg gtcgcgacat agcgccgcac cagtacgcgc
     4201 ccgcgcgcta gccagccgcc agattg
//
LOCUS       DATCBJ010000187         4849 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_8172, whole genome shotgun sequence.
ACCESSION   DATCBJ010000187 DATCBJ010000000
VERSION     DATCBJ010000187.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 4849)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4849)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4849
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8172"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(147..368)
                     /locus_tag="VII50_03795"
     CDS             complement(147..368)
                     /locus_tag="VII50_03795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5049999.1"
                     /translation="MGTIAVIGEAVRTAGFGLAGAIVFECETPDEVRHAWDALPDHVL
                     AVILTPAADAALRPHAGREASLPVVVMPS"
     gene            complement(375..797)
                     /locus_tag="VII50_03800"
     CDS             complement(375..797)
                     /locus_tag="VII50_03800"
                     /inference="COORDINATES: protein motif:HMM:NF012365.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase subunit C"
                     /protein_id="HEY5050000.1"
                     /translation="MSGWLIAVPLAVVATVLLMATLRRNRIVATRILLAANGILLLAA
                     LVLIVLALTAQPSSAATVAAAGTAKVLNGSALLGAGIAIAGSSIGGGIAVAYTGSAAL
                     AAMSERPEMFGRAMVVVGLAEGIAIYGLIVAIILVGKA"
     gene            complement(782..2212)
                     /locus_tag="VII50_03805"
     CDS             complement(782..2212)
                     /locus_tag="VII50_03805"
                     /inference="COORDINATES: protein motif:HMM:NF013647.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033179 - proton-transporting V-type
                     ATPase, V0 domain [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0046961 - proton-transporting ATPase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase 116kDa subunit family protein"
                     /protein_id="HEY5050001.1"
                     /translation="MHWRDGWTPVGMRRVGLLAPEDTVRELLVRVARAGVVQVPSSSP
                     ESATGPASVLLRRTQHKPQTAVLAAQAPDLRWCEEHDRLDLAAGEAQLEAVAESGVRH
                     GQAVGFVGWAPTNSLASLAEDLTPIGGTVVAIRRPRGSQPPTLLAAGSARRAFAPLVE
                     TYATPPYRDLDISVLAGLAYVAMFGMMFADVGHGALLLALALTARFGHWRRLDIVRPV
                     WVMVAGAGTLSMVFGAVYGEFFGPTHAFPVLWLSPLDQPVRLLSVAIAVGALLLAAAY
                     AVASVNRFREGGWQSAVYASSGLAGAAMFVGLGIMTGGLYIHIVVLTIAGAVLATAAL
                     GVAFVGFFVEAGGGFDGAAQAGVESFDGVVRLGSNVVSFARLAAFGMTHAALGQVVWQ
                     STVGASRHGPFGVGAAVVIFVVGNTITFSLEALIAGVQALRLEYYELFSRIFIAEGEP
                     FRPWHLSVESSVPVADSSLEASCLVG"
     gene            complement(2199..3116)
                     /locus_tag="VII50_03810"
     CDS             complement(2199..3116)
                     /locus_tag="VII50_03810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5050002.1"
                     /translation="MTGAWVGADVRAVAMSRRTVGRAGAASIAACADLTAALAQLQSS
                     PYARDVRPSHDLANAQRAVASTLLWNVRVIAGWVPASGVGMLRIAMAWFEILNLEARL
                     RPADRGPVVPQFEMGSLASTPGGSESVTTGNVLDRLRTTWWGTPSENSLRAVGLAMRS
                     RLVDRASSGLPAARAWAAGAAALMVAREQVVAGNRLPPDVRRNLAHTLGQPALDADTV
                     DSLREALPERARWVLDNVTHPEDMWRAECGWWARVDADGRELLRGSSFGPQPVVGAVA
                     VMAADAWRVRAALHAAALGGAMEAFDALA"
     gene            complement(3113..3574)
                     /locus_tag="VII50_03815"
     CDS             complement(3113..3574)
                     /locus_tag="VII50_03815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5050003.1"
                     /translation="MPMLRDILDRFRPAPGPGRPGPVGVPSSATLTCADELEAVFAAL
                     DDVVARCDAIREEGRRQAAEIRRAADIRLASLGSQAATSAAQTRAAAVASVKAAGRAR
                     QAVELAAAQGEAARISADGVERMTPYIAAAVSKVRNLPGSHTPTARVGDGS"
     gene            complement(3637..4521)
                     /locus_tag="VII50_03820"
     CDS             complement(3637..4521)
                     /locus_tag="VII50_03820"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEY5050004.1"
                     /translation="MTADHSPIVVGVDDSPASRSALYWALGEATSRQSPLEVVFAWRS
                     MYRTWPGGPGLIDLAVASREYGEARLASLLTRVRTSDPQASVTGQLLEGRPSEVLLKA
                     ASAADAQMLVVGARGVGGFDGLLLGSVGLHVATNAPCPVVIVRVARRAVGPIVVGIDG
                     SSESKAVLSAAFDEASVRKARLDVIHVLYVHSEAEGVPDRAGALAMARSDASESMQEL
                     LADAASDYPDVKVSTGLPTGYPAEILINASAEASMVVMGSHGGGGFADMRLGSVAHAV
                     VHHARCPVMLLRRRAALL"
BASE COUNT          904 a         1679 c         1565 g          701 t
ORIGIN      
        1 ggcttgatcc gcggcgtcgc gtgctgcgtc caattcggtc gctgtctcac tgcgcatacg
       61 cgcgacgcat gccgccgcct ccgcgtgggc gcgggccagt agcgcgttcc gcaagggctc
      121 gagcgcctgc tcgtcgacga ctgatctcac gatggcatca ccacaaccgg caggctcgct
      181 tcgcgaccgg cgtgtggtcg cagcgccgcg tcggccgccg gggtgaggat gaccgccagt
      241 acgtggtcgg gcagcgcatc ccatgcgtgc ctaacctcgt ccggagtctc gcactcgaat
      301 acgatcgcgc cggccagacc gaagcccgcg gtccggactg cttcgccgat caccgcgatg
      361 gtgcccatcg tccctcacgc cttcccgacg aggatgatgg cgacgatcag gccatagatc
      421 gcgatgcctt cggctaggcc gaccaccacc atcgcgcgac cgaacatctc cggtcgctca
      481 ctcatcgcgg ccaatgcggc ggagccggtg tatgccaccg cgatcccacc gccgatcgac
      541 gacccagcga tggcgatccc ggcaccgagc aacgcacttc cgttgaggac cttcgcggtg
      601 ccggcagcag cgacggtcgc tgccgacgac ggctgcgccg tcaaggcaag cacgatgagg
      661 acgagcgcgg ccaatagcaa tatgccgttg gcagccaaca gaatccgggt cgccacgatg
      721 cggttacggc gaagcgttgc catcaggaga acggtggcca caaccgcaag gggcactgcg
      781 atcaaccaac cagacatgac gcctccaaag acgaatcagc aaccggaacg ctcgactcga
      841 ccgacaggtg ccacggtcgg aatggttcgc cctcggcgat gaagatgcgg gaaaacaact
      901 cgtagtactc cagacgcaat gcctgtacgc ctgcgatcag tgcctccaat gagaacgtga
      961 ttgtgttgcc gacgacaaag atcacgacag ctgcgccaac gccgaacggc ccgtgcctac
     1021 ttgcgccgac tgtcgattgc catacgacct ggccgagtgc ggcgtgagtc atgccgaacg
     1081 cggcaagccg ggcgaagctg acgacattcg agccgagacg cacgacgcca tcgaacgact
     1141 cgacaccggc ctgagccgcg ccatcaaacc cgcccccggc ttcgacgaag aagccgacga
     1201 aagcaacgcc cagtgcggcg gtggcaagca cggcgccggc gatcgttagc accacaatgt
     1261 ggatgtacag accacccgtc atgatcccga ggccgacaaa catggccgcg ccagccaggc
     1321 cggacgaggc gtaaaccgca gactgccaac caccctcgcg gaaccgattc acactcgcca
     1381 cggcgtacgc cgccgcgagc aggagcgctc cgactgcgat cgcgaccgac agcagtcgaa
     1441 cgggctggtc gagaggcgaa agccagagca ccggaaacgc atgcgtaggt ccgaagaact
     1501 cgccgtagac ggcaccgaac accatgctca aggtgccggc accggcaacc atgacccaga
     1561 cggggcgcac gatgtccagt cgacgccagt gcccaaaacg tgccgtgagc gcgagcgcca
     1621 gcaacagcgc gccgtgcccg acgtcggcga acatcatgcc gaacatggcc acgtacgcga
     1681 gcccggccag gaccgagatg tcaaggtcac ggtagggagg cgtcgcatac gtctcgacga
     1741 gcggcgcaaa ggcgcgtcgt gcgctgccgg ctgcgagcag cgtggggggc tgcgatccgc
     1801 gcgggcgccg tatcgcaacg accgtgccgc ctatcggcgt gaggtcctcg gccaagctgg
     1861 cgagcgagtt cgtcggggcc caacccacga agccgacggc ctggccgtgc ctgaccccac
     1921 tctccgcgac ggcttccagc tgcgcctcac cggcggccag gtcaagtcgg tcgtgttcct
     1981 cgcaccaccg caggtcgggc gcctgtgccg ccaataccgc ggtctgcggc ttgtgctgcg
     2041 tgcgtcgaag aaggacgctc gcggggccgg ttgccgattc cggcgaggac gacggcacct
     2101 gcacgacgcc tgctcgagca actcgaacca gtaactcgcg gacggtgtcc tccggcgcga
     2161 gcaacccgac acgccgcatc ccgacgggag tccaaccgtc acgccagtgc atcgaatgcc
     2221 tccatcgcgc caccgagcgc cgccgcatgc aaagcggccc gcactcgcca ggcgtcagcg
     2281 gccatgaccg caacggcgcc gaccaccggt tggggtccaa acgacgatcc gcggagcaac
     2341 tcgcggccgt ccgcgtcgac gcgggcccac cagccgcact ctgctcgcca catgtcttca
     2401 gggtgcgtca cgttgtccag gacccacctc gcacgttctg gcagtgcttc gcgcagcgaa
     2461 tcgacggtgt cggcgtccaa cgcgggctgg ccaagcgtgt gtgcaaggtt gcgtcgaacg
     2521 tcgggaggca acctgttacc ggcgaccacc tgctcgcgcg caaccatcag cgccgcagct
     2581 cccgcggccc acgcacgagc tgctggcaag ccggagctag cgcggtcgac gagtcgactg
     2641 cgcattgcca ggccgacagc cctcagcgag ttctctgaag gcgttcccca ccatgtggtc
     2701 cggagacggt cgagcacatt gcctgtcgtg actgattcgg aaccaccggg agtggaggcc
     2761 agactgccca tctcgaactg tggcactact ggcccgcgat ccgcaggacg aagacgcgcc
     2821 tcgaggttga ggatctcgaa ccaggccatc gcaatccgca gcatgccgac accacttgcc
     2881 ggcacccatc cggcgatcac acggacgttc cacaacaacg tcgacgcgac ggcgcgctgg
     2941 gcgttggcga ggtcatgtga tggccggacg tcacgtgcgt acggcgagct ctgcagctgg
     3001 gcgagcgctg ctgtcaagtc cgcgcacgca gcgatggacg ctgcgccggc tcggccgacg
     3061 gtgcgacgcg acattgcgac tgcgcgcacg tcagcgccaa cccatgcacc ggtcacgacc
     3121 catcacccac acgagcggtg ggggtatgcg agccaggcag gttgcgcacc ttggacactg
     3181 cggcggcaat gtacggcgtc atccgctcga ctccgtcggc gctgatccgc gctgcttcgc
     3241 cttgggcggc ggcaagttcg acagcttgtc gggcccgacc ggccgccttc accgaagcga
     3301 ctgccgcggc ccgggtttga gctgcggaag ttgctgcctg cgatcctaag ctcgcgagtc
     3361 gaatgtcggc ggctcgccgg atttctgctg cttgccgccg cccttcctcg cgaatcgcgt
     3421 cgcagcgcgc gacgacgtcg tccaaggcag caaagactgc ctcgagttcg tctgcacagg
     3481 tcagcgttgc ggacgacggc acacccaccg gtccggggcg acctgggccc ggagcaggcc
     3541 gaaatcgatc gaggatgtcg cgcagcattg gcatgacaga tcacgtgcgt atcggaggag
     3601 ccgggccaac ttcggtcgac gaccggcaaa gcgagctcat aacagcgcgg cccggcggcg
     3661 tagcagcatc acggggcagc gcgcatggtg caccaccgca tgggcgacag agccgagcct
     3721 catgtccgcg aaaccacccc caccgtggga tcccatgacg accatgctcg cctcagcgct
     3781 cgcattgatc agaatctccg cggggtatcc cgtcggaagt cccgtgctga ccttgacgtc
     3841 gggatagtcg gaggcggcgt cggcaagcag ttcctgcatc gactcgctcg cgtcggacct
     3901 tgccatcgca agagccccag cccggtccgg caccccttcc gcctcggagt gcacgtacag
     3961 gacgtggatg acgtccaacc gcgccttgcg caccgatgct tcatcgaacg ccgccgacag
     4021 gactgccttg gattcgctcg acccatcgat accgaccacg atcgggccaa cggcgcgacg
     4081 ggcgacgcgc acgatgacga ccggacacgg cgcgttcgtc gcaacgtgca gtcccaccga
     4141 gcctaggagc agtccgtcga agccgccgac gccgcgtgct ccgaccacca gcatctgggc
     4201 atcggccgcg gaagctgctt tgagcaacac ctcgctcggg cggccctcga gaagttgtcc
     4261 ggttaccgag gcctgcggat cggaggtccg cacgcgggtg agcagggacg cgagccgcgc
     4321 ttcgccgtat tctcgcgaag cgaccgcaag gtcgataagg ccgggaccgc cgggccaggt
     4381 gcggtacatg ctgcgccagg cgaagacaac ctcgagcggc gactgccgcg acgtggcctc
     4441 gccgagcgcc cagtagagcg cgctccggct ggctggcgag tcatcgactc cgacgacgat
     4501 ggggctatgg tcggcggtca cgagatgcct ccttgctggc tccgagttgg agccgagcga
     4561 gatatcactc gtagcaaacg actgctcgac tggcagacgc cacgggctaa agtcccgtgg
     4621 cgtggcgtcg tcgggcctct gcagttcctc gattggcgtt gctgacgtcg aacccatcgg
     4681 caaacgttcg atccgcgcag ggggatggaa gtcaggagct aagactcgtg tcgccgggcc
     4741 gttttcctct gcgtcgatga agcggccgcg cgcacagtgg cgatacaacg tcctcaggag
     4801 gcgagtgtca tgacgtcacg actcggcttc gatcagcttg ggttgccga
//
LOCUS       DATCBJ010000188         3788 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_472023, whole genome shotgun sequence.
ACCESSION   DATCBJ010000188 DATCBJ010000000
VERSION     DATCBJ010000188.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 3788)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3788)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3788
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_472023"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..266
                     /locus_tag="VII50_03825"
     CDS             <1..266
                     /locus_tag="VII50_03825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009085324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HEY5050005.1"
                     /translation="TLPVEQRAAIVLVDIEGYPVDEVAAMLEVPSGTVKSRCARGRAK
                     LVPLLSHLRGEPDPGDQPGEQARNPTEADRVEPPMNPPREDLR"
     gene            263..1381
                     /locus_tag="VII50_03830"
     CDS             263..1381
                     /locus_tag="VII50_03830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5050006.1"
                     /translation="MNTPHPDLETLAELADREISPASDTAAASNAAADPRTAETLAHV
                     RGCAACTADLEALRGIRETLRALPPVPMPPEVTERIEAALLAARAPALPTAPTGVSDT
                     VVPLRQRSRSASRQRSGGSAARWRTLPYGAAASIVVVLALVIVGAVSIGRGGGTKKSA
                     APAVAAAGTSAASVTIASGTNYTAADIRSQVTSVVTSNVPGAVADFPGLTFGVSRPAA
                     GSAAAPVAPAAAAASSAAAAATSAAPSASASAAAAAPSSPALAPIPAASPAAGAPING
                     GTAESAASTPRSPITAPAGPLADPAALAQCVQVLAGKAEQPLLVDYATFDGAPSTIIV
                     LPDPAVAHKLDVYVIADTTNCADQNFTFETFIGPSPGP"
     gene            1488..2417
                     /gene="trxB"
                     /locus_tag="VII50_03835"
     CDS             1488..2417
                     /gene="trxB"
                     /locus_tag="VII50_03835"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006680606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADP) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HEY5050007.1"
                     /translation="MNVRELIIIGSGPAGYTAALYAARADLHPLVFEGAIAAGGALMT
                     TTEVENFPGFPDGIMGPELMESLRKQAARFGAELVTDDVTEVDLTGAVKVVHVGEQVF
                     YSRAVILATGSKYRYLGLDNEARLLGHGVSACATCDGFFFRDQDIAVVGGGDSALEEA
                     TFLSKFARSVTVVHRREALRASKIMQDRAYANDKVGFRWNSEVTDVIGDDKVAGVTVR
                     DTVTGAKDQLPVTGLFVAIGHDPRSELFRGQVDLDDAGYVKVAQPSTRTNLDGVFACG
                     DLVDHTYRQAVTAAGTGCAAALDAERWLAAQHD"
     gene            2481..2804
                     /gene="trxA"
                     /locus_tag="VII50_03840"
     CDS             2481..2804
                     /gene="trxA"
                     /locus_tag="VII50_03840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HEY5050008.1"
                     /translation="MGATKSVTDETFATDVLSSDTPVLVDFWAEWCGPCRMVAPVLEE
                     IANEHADKVRVVKLNIDENPETARAYSILSIPTLNVYKGGQVVKQIIGAKSKSALLKD
                     LEAFL"
     gene            2984..>3788
                     /locus_tag="VII50_03845"
     CDS             2984..>3788
                     /locus_tag="VII50_03845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding protein"
                     /protein_id="HEY5050009.1"
                     /translation="MLELRHRLRQLDLLINDEAAPGTRASQHGDPGDLFDEACDHAVR
                     SFQQQRGLRIDGVVDRETYRSLDEARWRLGDRTLSFAVRHPYVGDDVAALQHRLTDMG
                     FDCGRADGVFGARTENALREFQRNVGLPADGTCGPATFRALRQLARTVVGGKANELRE
                     YERLHHGGPTMAGKLVVIDPGHGGRDRGSTNGTLDEAAIVDDIANRLEGRLAAAGAQA
                     FRTRGGEGVLDDTDALERRSDAERGAFANAAEADLLVSLHIDGDLNPGCH"
BASE COUNT          630 a         1343 c         1221 g          594 t
ORIGIN      
        1 caacgttgcc cgtcgaacag cgggccgcga tcgtgctcgt cgacatcgag ggctatccgg
       61 tcgacgaggt cgccgcgatg ctcgaggtgc cttcgggcac ggtcaagagc cggtgcgcgc
      121 gcggccgggc caagttggtc ccactgctat cgcatctaag gggcgaacca gaccctggcg
      181 accagcccgg cgagcaagcg cggaacccca ccgaggcgga ccgcgtcgaa ccaccgatga
      241 acccaccccg agaggacctc cggtgaacac cccgcacccg gacctcgaga cgcttgcgga
      301 actcgcagat cgcgagattt ctccagcctc cgacacggca gccgccagta atgccgcggc
      361 cgacccgcgc accgcagaga ctctcgccca tgtcaggggt tgcgctgcgt gcaccgcgga
      421 cctagaagcc ctgcgcggca tccgggagac cttgcgtgcg ctgccgccgg tgccgatgcc
      481 gccggaggtc accgagcgca ttgaagccgc tctgcttgcc gcccgcgcgc cagcgctgcc
      541 caccgcgccg accggggtgt ccgacaccgt ggttccgctt cgacagcggt cgaggtcggc
      601 ctcgcggcag cgttccggcg gctcggcggc gcgctggcgg acactgccct atggggccgc
      661 ggcgtccatc gtcgtcgtgc ttgcgctggt gatcgtcggt gccgtgagta tcgggcgcgg
      721 cggcggaacg aaaaagtcgg ccgctccggc ggtagccgcc gctggtacgt ccgccgcttc
      781 ggtgacgatc gcctctggca ccaactacac cgctgccgac atccgctccc aggtcacctc
      841 cgtcgtgacc agcaacgtcc caggagcagt ggccgacttt ccgggcctga cctttggtgt
      901 ctcgcgacca gccgccgggt ccgcggccgc ccccgtggcc cccgcagccg ccgcggcgtc
      961 gtccgccgca gccgccgcaa cgtccgctgc cccctcggcg tccgcttccg ccgccgccgc
     1021 agcgccgagt tcaccagcgc tcgcgccgat cccggcagca tcgccggctg cgggagcccc
     1081 aataaacggc gggacggcgg agagtgcggc cagcacgccg cgctccccca tcacggcacc
     1141 ggccgggcca ttggcggacc cggccgcctt agcgcaatgt gtccaggtcc tggctgggaa
     1201 ggccgagcaa cccctgctcg tcgactacgc aaccttcgac ggtgccccgt cgacgatcat
     1261 cgtgctcccc gaccctgccg tcgcgcacaa gctcgacgtg tacgtgatcg cagacacgac
     1321 gaactgtgca gaccagaatt tcacgttcga aacgttcatc ggcccatctc ctggcccctg
     1381 atgtctgggt gacctcgcga gcctgcccgg tcgggaatcg cggacgccta ggatctgttg
     1441 aaggccctag tcgcccgatc ggcgactgtc ttaagaggta ggtccttgtg aacgtccgtg
     1501 aactcatcat catcggatcc ggcccggccg gttacaccgc ggcgctgtac gccgcgcgcg
     1561 ccgacctgca cccattggtt ttcgagggcg cgattgcggc cggcggcgca ctgatgacga
     1621 ccactgaggt cgaaaacttc ccgggctttc ccgacgggat catgggaccc gagctgatgg
     1681 agagcctgcg caagcaggcg gcccgcttcg gcgccgaact ggtcaccgat gacgtcaccg
     1741 aggtcgacct caccggagcg gtcaaggtcg ttcatgtcgg cgagcaggtc ttctactcgc
     1801 gcgcggtgat cctggcaacg ggttcgaagt atcgctactt gggactcgac aacgaagctc
     1861 gactgctcgg ccatggtgtc tctgcttgtg cgacttgtga tggattcttc tttcgtgacc
     1921 aagacatcgc ggtcgtcggt ggcggcgact ccgctctcga ggaagccacg tttctgagca
     1981 agttcgctcg cagtgtcacc gtcgtgcacc gtcgtgaggc gctgcgtgcg agcaagatca
     2041 tgcaggaccg tgcatacgca aacgacaagg tgggcttccg ctggaactcc gaggtcaccg
     2101 atgtgatcgg cgacgacaag gtcgccggcg tcacagttcg cgacaccgtc acgggtgcca
     2161 aggatcagct accggtgacc ggcctgttcg ttgcgatcgg ccacgacccg cgcagcgagc
     2221 tgttccgcgg ccaagttgat ctagacgatg ctggctacgt gaaggtcgcg cagccctcga
     2281 cgcgcaccaa cctcgacggg gtcttcgctt gcggcgacct ggtcgaccac acctaccggc
     2341 aggcggttac cgcagccggc actggctgtg cggccgcgct cgatgcggaa cgctggctgg
     2401 ccgcgcaaca cgactaaaca gcacggctgc ctcaccagtt tcatccatca catcactgtt
     2461 caacctgaca ggagcccact gtgggagcca ccaagtccgt caccgacgag acctttgcga
     2521 ccgatgtcct cagcagcgac accccggtcc ttgtcgactt ctgggccgag tggtgcggtc
     2581 cctgccgcat ggttgcgccg gtcctcgagg agatcgcgaa cgagcacgcg gacaaggttc
     2641 gggtagtcaa gctcaacatc gatgagaacc ccgagaccgc ccgcgcctac tcaattctgt
     2701 cgatccccac gctcaacgtg tacaagggcg gtcaggtcgt gaagcagatc atcggggcca
     2761 agtcgaagtc tgccctgttg aaggatctgg aagcgttcct ctagcccgct accatggccg
     2821 ctccacgccg gtaatctcgc cgcggcggca cactgcggta aacgccggaa ccgcgccggg
     2881 cgtcaatcac ccaccggagc ggtcgacccg aacgaggacg ccgatgtaac agcagcacga
     2941 gcgggtcgac gagccgctgc ggctcggcga ccgcggactc gccgtgctcg agctgcggca
     3001 tcggctgcgc cagctcgatc tgttaatcaa cgacgaggcc gcgccgggca cccgcgccag
     3061 ccaacacggc gacccaggtg acctgttcga cgaagcctgc gaccacgcgg tccgtagctt
     3121 ccaacagcaa cgcggcctgc gcatcgacgg cgtcgtcgat cgcgagacct accgcagcct
     3181 cgacgaggcc cgctggcggc tcggggaccg cacgctgtcc ttcgccgtcc ggcatcccta
     3241 cgtaggcgac gatgtcgcgg cccttcaaca ccggctcacc gacatggggt tcgactgtgg
     3301 tcgggctgat ggtgtgttcg gtgcccgcac cgaaaacgcc ctgcgtgagt ttcagcgcaa
     3361 cgtcgggctg ccggccgacg gcacctgcgg ccccgccacg ttccgggcgt tgcgccagct
     3421 cgctcgcacg gtcgtgggtg gcaaagccaa cgagctgcgc gagtacgaac ggcttcatca
     3481 tggcgggccg acgatggccg gcaagctcgt ggtgatcgac cccggccatg gcgggcgcga
     3541 ccgagggtca accaacggca cactcgacga ggccgcgatc gtcgacgaca tcgccaaccg
     3601 gctcgagggc cggctcgcag ctgctggcgc ccaggcattt cggacccgcg gcggcgaggg
     3661 tgtcctggac gacaccgacg cgctcgaacg tcggagcgac gccgagcgag gggcattcgc
     3721 gaacgccgcc gaagccgacc tgctcgtctc cttgcacatt gacggcgacc tcaacccggg
     3781 ttgccacg
//
LOCUS       DATCBJ010000189         2577 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_4781, whole genome shotgun sequence.
ACCESSION   DATCBJ010000189 DATCBJ010000000
VERSION     DATCBJ010000189.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2577)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2577)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2577
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4781"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(62..865)
                     /locus_tag="VII50_03850"
     CDS             complement(62..865)
                     /locus_tag="VII50_03850"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009079746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEY5050010.1"
                     /translation="MTSEVGRRAMGADETVQANRRWWDAAADDYQSEHGEFLGGRDAA
                     HFIWGPEGLDEADVHLLGSIADLVGRRVLEVGCGAAQCSRWLAAQGAVCIAIDLAMRQ
                     LTHAAPHPGVRLIQADARAIPLAHCSVDLAFSAYGALPFVANSGDVLREAFRIVRPGG
                     RFVFSVTHPIRWALPDDPGPAGLTVRSSYFDRTPYVEADADGRATYVEHHRTLGDWVR
                     DIVGAGFDLTELVEPEWPDTNLRLWGGWSPLRGRLIPGTAIFVCDKPGG"
     gene            1128..>2577
                     /gene="rpsA"
                     /locus_tag="VII50_03855"
     CDS             1128..>2577
                     /gene="rpsA"
                     /locus_tag="VII50_03855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019511500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HEY5050011.1"
                     /translation="MTSSTYVRPETVLGAAPHVSPQVAVNDIGSSEDFLAAIDETIKY
                     FNDGDIVDGTIVKVDRDEVLLDIGYKTEGVIPSRELSIKHDVDPNDIVKVGDHIEALV
                     LQKEDKEGRLILSKKRAQYERAWGTIEQIKENDDIVTGTVIEVVKGGLILDIGLRGFL
                     PASLVEMRRVRDLQPYVGRELEAKIIELDKNRNNVVLSRRAWLEQTQSEVRQTFLTTL
                     KKGQIRNGVVSSIVNFGAFVDLGGVDGLVHVSELSWKHIDHPSEVVEVGNEVTVEVLD
                     VDMDRERVSLSLKSTQEDPWQAFARTHAIGQVVPGKVTKLVPFGAFVRVEEGIEGLVH
                     ISELAERHVEIPEQVVQVGDEIFVKVIDIDLERRRISLSLKQANESSGDSAVTDSHFD
                     PTLYGMAANYDAEGNYIYPEGFDSETNEWLPGHEEKQAEWERQYAEARARFEAHQSQI
                     VEAQKAEAAAGTEEDAASSSVTSSTSSTSASSS"
BASE COUNT          472 a          841 c          856 g          408 t
ORIGIN      
        1 agtttctcca cgtctgattc gttcggcgtt cagtgggacc cggcggcagt cagctccttg
       61 gctagccgcc gggtttgtca cagacgaaga tcgcggtgcc gggaatcagt cggccgcgca
      121 gcggtgacca accgccccac aggcgcaagt tggtgtctgg ccactccggc tcgacgagct
      181 cggtgaggtc gaagccggcg ccgacgatgt cgcggaccca gtcgccaagc gttcggtggt
      241 gctcgacgta ggtcgcccgc ccgtcggcgt ccgcttcgac atacggcgtg cggtcgaagt
      301 acgacgaacg aaccgtcaga ccggcaggcc caggatcgtc cggcagcgcc cagcggatcg
      361 ggtgggtcac cgagaagacg aatcggccgc cggggcgcac tatccgaaac gcctcgcgca
      421 gcacgtcgcc ggagttggcc acgaacggca acgcgccgta ggcgctgaac gcgaggtcga
      481 cgctgcagtg cgcgagtggg atcgcgcgag cgtcagcctg aatgagccgg acgccggggt
      541 gcggggcggc gtgggtcagc tggcgcatcg ccaggtcgat cgcgatgcac accgcgccct
      601 gggcggccaa ccacctagag cactgcgcgg caccgcagcc gacctcgagc acccggcggc
      661 cgaccaggtc ggcgatcgag ccgagcaggt gcacgtcggc ttcgtcgagg ccctcgggac
      721 cccagatgaa gtgggcggca tcgcgcccgc cgaggaactc gccgtgctcg gactggtagt
      781 cgtcggcggc ggcatcccac caacgccgat tggcctgcac cgtctcgtct gcgcccattg
      841 ctcgacgtcc gacctcggag gtcacagccg cctccttctg catgatcacc gggatgcacc
      901 cgggcgcgga aggcccggat tgaccggttc acagcgtgcc acgtaaagtg ggcggtgcgt
      961 gtgggcctgt gtgtacctcg gtatggagca ggttcgcgat cgcttcggtc gacttcggta
     1021 acccctcccg cgcttaacag acgcagtgtg gccgccgttt cgcggctgag acgacgggcc
     1081 tgggcgcgcc tgccaacccg ttgtccgcga ccggagtcca cccccttatg accagcagca
     1141 cctacgtacg acccgagacc gtcctcggcg ccgcccccca cgtgtctccc caggtggcgg
     1201 tgaacgacat cggatcctcc gaggatttcc tcgccgcgat cgatgagacg atcaagtact
     1261 tcaacgacgg cgacatcgtc gacggcacga tcgtgaaggt cgaccgcgac gaagtgttgc
     1321 tcgacatcgg ctacaaaacc gagggcgtca tcccctcccg ggaactgtcg atcaagcacg
     1381 acgtcgaccc caatgacatc gtcaaagtgg gtgaccacat cgaggccctg gttctccaga
     1441 aggaggacaa ggagggtcgg ctgatcctgt ccaagaagcg cgcccagtac gagcgcgcct
     1501 ggggcacgat cgagcagatc aaggaaaacg acgacatcgt caccgggacc gttatcgagg
     1561 tcgtcaaggg cggcctgatc ctcgacatcg gcctgcgcgg cttcttgccg gcgtccctcg
     1621 tcgagatgcg acgggtacgc gacctgcagc cctacgtcgg gcgcgagctt gaagcgaaga
     1681 tcatcgagct cgacaagaac cgcaacaacg tcgtgctctc gcgtcgcgcg tggctcgagc
     1741 agacccagtc cgaggttcgc cagaccttcc tgacgacctt gaagaagggc cagattcgca
     1801 acggcgtcgt cagctcgatc gtcaacttcg gtgcgttcgt cgaccttggt ggcgtcgacg
     1861 gtctggtcca cgtctctgag ctgtcgtgga agcacatcga ccacccgagc gaggtcgtcg
     1921 aggtcggcaa cgaggtcacc gtcgaggtgc tcgacgtcga catggaccgc gagcgcgtct
     1981 cgctgtcgct gaagtcgact caggaagacc cgtggcaggc cttcgcgcgt acccacgcga
     2041 tcggccaggt cgtgccaggc aaggtcacca agctggttcc cttcggtgcg ttcgtgcgcg
     2101 tcgaagaggg catcgagggt ctggtgcaca tctccgaact cgccgagcgg cacgtcgaga
     2161 ttcccgagca ggtcgtgcag gtgggcgacg agatcttcgt caaggtcatc gacatcgacc
     2221 tcgagcgccg gcgcatcagc ttgtcgctga agcaggccaa cgagagcagt ggcgacagcg
     2281 ctgtcaccga cagccacttc gatccgacgc tttacggcat ggcggcgaac tacgatgccg
     2341 agggcaacta catctatccc gagggcttcg actccgagac caacgaatgg ctgccggggc
     2401 acgaagagaa gcaggccgaa tgggagcgtc agtacgccga ggcgcgcgcg cggttcgaag
     2461 cgcaccagag ccagatcgtc gaggctcaga aggccgaagc ggccgccggc acggaggagg
     2521 atgcggcttc gtcgagtgtg acctcttcca cttcgtcgac atcggctagc tcgtcga
//
LOCUS       DATCBJ010000190         7180 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_335117, whole genome shotgun sequence.
ACCESSION   DATCBJ010000190 DATCBJ010000000
VERSION     DATCBJ010000190.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 7180)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7180)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7180
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_335117"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..280)
                     /locus_tag="VII50_03860"
     CDS             complement(<1..280)
                     /locus_tag="VII50_03860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5050012.1"
                     /translation="MRSTPVEPEPPSATAAVLATVAAALETGAVLLDDDQVLLANEAA
                     LALRVVHGHELTSRMLARITREARRTGLRIAQDIDLPWGSSFRAVHVVA"
     gene            475..1125
                     /gene="phoU"
                     /locus_tag="VII50_03865"
     CDS             475..1125
                     /gene="phoU"
                     /locus_tag="VII50_03865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011718930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HEY5050013.1"
                     /translation="MRDAYHDELDAITDQLVEMTELVGQAITQATEALLDSDLSIAES
                     VIDGDDSVDIIRENVDRRTVDLIARQQPVAGDLRRLTTGLRIASDLERSGDLAVHVAK
                     LARRKFPACAVPNELQATIREMGEVAADIVGKAGHVVAGRDADRAAELESDDDVMDHL
                     HRTLLLTMLAPEWPYGVDTAIDLALVGRYYERFADHAVAVAKQVVYLVTGETAMRN"
     gene            complement(1324..1590)
                     /locus_tag="VII50_03870"
     CDS             complement(1324..1590)
                     /locus_tag="VII50_03870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5050014.1"
                     /translation="MFAWNVDPCALSEPVPHAGALPAAAGALDAAVDAAAVVAGAADE
                     LAVVGVAAALVDALVVFLLLLHAAAANNTPAISRLVAIREVLTR"
     gene            1589..2509
                     /gene="pstS"
                     /locus_tag="VII50_03875"
     CDS             1589..2509
                     /gene="pstS"
                     /locus_tag="VII50_03875"
                     /inference="COORDINATES: protein motif:HMM:TIGR00975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0042301 - phosphate ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HEY5050015.1"
                     /translation="METQWSKDFATQCSGAQVNYQATGSGAGITAIGNGTADFAGSDV
                     TMTAAQQTAANTACGSTAITIPVTVGGVAIVYNLSAVPKLQLSATDLAGIFSGKITKW
                     NDPALATDNPGVTLPSTPISVFYRNDSSGTTQVYTSFLVADAGSAWTLGSSKTLTFFP
                     GATGKPKSAGVAAAVSQTTGGITYDEESYAKGQNLPTALVKGTGTSFVAPTTDQVSQS
                     IADGFNITGTGNDLAGSLAFTKMTGYPISTVSYAIACTKYKDAAKGASVKSFLYYAVS
                     KGQAAADGLGFAALPSSLVSKDEASISAIS"
     gene            2506..3489
                     /gene="pstC"
                     /locus_tag="VII50_03880"
     CDS             2506..3489
                     /gene="pstC"
                     /locus_tag="VII50_03880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011718932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HEY5050016.1"
                     /translation="MTQGTSAVPATAPGTPGRARRVEPFRAITFVAALFVLVILGAIA
                     VFLVVKAIPSLKDDKGNFLTTKVWNPDDTPPLFGIAVLVFGTVLSSVIALVIAVPVAL
                     GVAIFVVEYAPRRLATWLGYLIDMLATVPSVVYGLWGLVFLVPHIRGFQHFLASDFGW
                     IPLFKDPNNRTSVASQSIFVASIVLAIMVVPVIAAISREVLRQVDPNLKEAALALGAT
                     RWEMVRTALLPPSRPGIIGGIMLGLGRALGETIAIALILGTSFAISFHILVPGGDTIA
                     ANIANTFGEASVTGRSALIASGLVLFILTFGVNLIARVVIHRSAVRQGTAT"
     gene            3486..4388
                     /gene="pstA"
                     /locus_tag="VII50_03885"
     CDS             3486..4388
                     /gene="pstA"
                     /locus_tag="VII50_03885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011718933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HEY5050017.1"
                     /translation="MSVATGQVSAPPNDLWKIRGRRAIVNRLMTGVMYLAFVVAVVPL
                     ALVIWYTVGKGLTRFSFNFLTHSMAGVGPDDIGGGISHAIVGTVEQLAIASVISVPLG
                     LLVAIHLNEYGQRGFSTVVRLLVDVMTGIPSIVAGLFILAFWVIGLHQGYSGFAGSMA
                     LAILMLPVVIRSSEEMLKLVPNSLREAAYALGIPKWRAILFIILPTARTGITTGVMLA
                     IARVTGETAPLLLTAFGTLFLNKNPTHGPQEALPLYVFSSAAAGTPADVNRAWAAAMT
                     LILIVVLLYVIARLLTRRDVLNRR"
     gene            4397..5170
                     /gene="pstB"
                     /locus_tag="VII50_03890"
     CDS             4397..5170
                     /gene="pstB"
                     /locus_tag="VII50_03890"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007032582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HEY5050018.1"
                     /translation="MAKRIEALGLSCYYGNHKAVENVTMTIEPKAVTALIGPSGCGKS
                     TFLRSLNRLHEVIPGAHITGQVLLDDRDIYAPGVDPVALRRSVGMVFQRPNPFPTMSI
                     YDNVAAGLKLGGSKGKDLDTVVERALTGAHLWAEVKDRLKKPALALSGGQQQRLCIAR
                     AIAVEPEVLLMDEPCSALDPISTLAIEDLIAELKDRYTIVIVTHNMQQASRVSETTGF
                     FTLAGAGQPGVLVEYDATSRIFSVPSDKRTEDYITGRTG"
     gene            complement(5211..6170)
                     /gene="lgt"
                     /locus_tag="VII50_03895"
     CDS             complement(5211..6170)
                     /gene="lgt"
                     /locus_tag="VII50_03895"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HEY5050019.1"
                     /translation="MRDYLPSPSQGVWHLGPVPIRAYALCLIAGIFVALFVTRRRWAA
                     RGHHPDDVSAIAMWAVPFGIIGGRLYHVITDPELYFKKGEHPIQALYIWRGGLGIWGA
                     VALGAVGAWIGCRRRGVPLRDFADALAPGLVLAQGIGRWGNYFNQELYGRPSGLPWAL
                     RIDPANRPPSTPTVALYQPTFLYESLWDVGVALLLVWADRRLALRRGRLFALYVLAYT
                     AGRGWVESLRVDHANHIAGLRLNDWTSILVFLGAATYLFLTRPGREDRQVIRDNNKPP
                     SPEQWESMFGPSEPDVVVIPAPPPPSQQSQPRPEPDRDSGGSG"
     gene            complement(6258..6989)
                     /locus_tag="VII50_03900"
     CDS             complement(6258..6989)
                     /locus_tag="VII50_03900"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009075754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HEY5050020.1"
                     /translation="MPPPGTALAQVAHWSASNPAAGITDATRKLGETGDVARRHEWAS
                     VTKLVTAVCVLRLVDEGAAISKPERRRVYSNAGFDLLGALVSSHTGVPFARYAAEAVF
                     GPLGMHDSSIEGTPSAGGVGPLVDLLRFARELLAPTLVAPATLALATAVAFPGLDGVL
                     PGFGRCAPNDWGLGFEIRDGKDPHWTGRNNSPSTFGHFGGSGSFLWIDPSLGLGCVSL
                     ADTPFGPWAASAWPVFSDAVVAEYS"
     gene            complement(6999..>7180)
                     /locus_tag="VII50_03905"
     CDS             complement(6999..>7180)
                     /locus_tag="VII50_03905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010157043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="arsenate reductase ArsC"
                     /protein_id="HEY5050021.1"
                     /translation="VITMGCGDACPVFPGKRYEEWILDDPAGQDVAAVRPIRDEVERR
                     VLALIDELKLPAASP"
BASE COUNT         1259 a         2427 c         2277 g         1217 t
ORIGIN      
        1 ccgcgaccac atggaccgca cgaaacgacg agccccaagg caggtcaatg tcctgggcga
       61 tgcgcagccc ggtccgccta gcctcgcgtg tgatccgggc cagcatgcgc gaggtcagct
      121 catgcccgtg gacgacccga agcgcgaggg cggcctcgtt ggcgagcaga acctggtcgt
      181 cgtcgagcag gaccgccccg gtctccaggg cggcggcgac ggtcgcgaga actgccgccg
      241 tcgccgacgg cggttctggc tccactggag tcgaccgcac aagccgaatg gtagggaacc
      301 gcagcgctgt caacaaacga actagggccg ttggccgact gttcaccctg cgttctgtag
      361 gctgtcgcct cgtcgcccgc cgccgtccat gcaagtggca gactctgccg gtggaatatg
      421 cgacgtttcc ggctttgcgg acggcgcagc cgggactgac ggaagggagt gggcgtgcgc
      481 gacgcctatc acgacgagct tgacgccatc acggaccagc tcgttgagat gaccgagttg
      541 gtcggccaag cgatcactca ggcgacggaa gcgctgctgg actcggacct gtccatagcc
      601 gagagcgtga tcgacggtga cgacagcgtc gacatcatcc gcgagaacgt tgatcggcga
      661 acggtcgacc tcatcgctcg tcagcagcct gttgcgggtg acctgcgtag gctcaccacg
      721 ggccttcgca ttgcctccga cctcgagcgc tccggcgatc tagccgtcca cgtcgcgaag
      781 ctggcccggc gcaagttccc cgcttgtgcg gtgccgaacg agctgcaggc caccatccga
      841 gagatgggcg aggtggccgc cgacattgtc ggcaaagctg gacatgtcgt cgcaggacgc
      901 gacgccgacc gcgcggccga actcgaatct gacgacgacg tcatggacca tctgcaccgg
      961 accctcctgc tgacgatgct ggctccggag tggccctacg gtgtcgatac agcgatcgac
     1021 ctggccttgg tgggccgcta ctacgagcgc ttcgccgatc acgcggtcgc cgtcgcaaag
     1081 caagtcgtgt atctggtcac cggcgaaacg gcgatgcgga actgagaccg ctcgctctgt
     1141 ggatgttcac cgcccgttca tccgcacgac gaaatccgtc cacttagctc gcctagtttc
     1201 gtggacgact ggacccattc ccgacctagc tctattcccg actggctcgc acacaactca
     1261 tcgtccgagc ccgaagctgg ccccgtccga caaagtcggt tcggttgacc gacacaggag
     1321 gagttacctc gtgaggacct cacgtatcgc cacaagcctg ctgatcgcag gtgtgttgtt
     1381 ggcggccgct gcatgtagca gcagcaagaa gaccaccagc gcgtccacga gtgcggcggc
     1441 cacgccgacc actgcgagct catctgcggc tccggccaca accgccgcgg catcgaccgc
     1501 cgcatcgagc gctcccgccg cagccggcag cgccccagcg tgtggcaccg gctcgctgag
     1561 tgcgcagggg tcgacgttcc aggcgaacat ggaaacccag tggagcaagg acttcgcgac
     1621 ccagtgctca ggcgcacagg tcaactacca ggcgaccggt tccggtgctg gcatcacggc
     1681 catcggcaac gggacggcgg acttcgccgg ctctgacgtc acgatgaccg ccgcacagca
     1741 gactgccgcg aacaccgcgt gcggcagcac ggcgatcacg atcccggtga cggtcggtgg
     1801 cgtcgcgatc gtctacaacc tttcggctgt gcccaagttg cagctttcgg cgaccgatct
     1861 ggccggcatc ttctccggca agatcaccaa gtggaacgac ccggcactcg ctaccgacaa
     1921 ccccggagtg acgttgccgt cgacgccgat cagcgtcttc taccgcaacg actcgtccgg
     1981 cacgacgcag gtctacacct cattcctggt ggccgacgca ggcagcgcgt ggaccctcgg
     2041 cagcagcaag acgctgacct tcttcccagg ggcgaccggc aagcccaagt cggcaggcgt
     2101 cgcggctgcg gtgagccaga ccaccggcgg catcacgtat gacgaagagt cctacgcaaa
     2161 gggccagaac ctgcccaccg cgttggtcaa gggaacgggc accagcttcg tcgccccgac
     2221 cacggaccag gtgtcgcagt cgatagcgga cggcttcaac atcaccggca ctggcaatga
     2281 tctggctggc tcgctagcgt tcaccaagat gaccggctac ccgatctcga cggtgtcgta
     2341 cgcgatcgcg tgcactaagt acaaggacgc cgccaagggt gcctcggtga agtccttcct
     2401 gtactacgcg gtgagcaagg gccaggctgc cgccgacggc cttggcttcg ccgcgctgcc
     2461 gagctcattg gttagcaagg acgaagcaag catctcggcg atttcgtgac ccaaggcact
     2521 tccgcagtcc ccgcgaccgc gcctggtacg cccggaaggg ctcgacgtgt cgagcccttc
     2581 cgggcgatca cctttgtcgc tgcactgttc gttctcgtca tcctgggcgc gatcgcggtt
     2641 ttccttgtgg tcaaggcgat tccgtcgctc aaggacgaca aggggaactt cctcaccacc
     2701 aaggtctgga acccagacga cactccgccc ctgtttggca tcgccgttct cgtcttcggc
     2761 acggtgctct cgagcgtgat cgccttggtg atcgccgtgc cggttgcgct aggcgtcgcg
     2821 atcttcgtgg tcgagtacgc cccgcgccgt cttgcaacct ggctcggcta cctcatcgac
     2881 atgctcgcta ccgtgccgtc ggtggtttac ggcctatggg gcctggtttt tctggttccg
     2941 cacatccgag gcttccagca cttcctcgcg agcgatttcg gctggatacc gctgttcaaa
     3001 gaccccaaca accgcacctc agtggcgagt cagtcgatat tcgtggcctc catcgtgctc
     3061 gccatcatgg tggtgccggt gatcgccgcg atttcgcgcg aagtgctgcg gcaagttgat
     3121 ccgaacctca aggaagccgc actcgcactg ggcgcgaccc ggtgggaaat ggttcgtacc
     3181 gcgttgctgc caccgagccg ccccggcatc atcggcggca tcatgctggg ccttggtcgc
     3241 gcgctcggtg agacgatcgc catcgccttg attctcggta ccagcttcgc catcagcttt
     3301 cacatcttgg tgccgggcgg cgacacgatc gccgccaaca ttgccaacac cttcggcgaa
     3361 gccagcgtca ccggccgctc ggcgttgatc gcgagtggcc tcgtgctgtt catcctcacc
     3421 ttcggtgtca acctgatcgc tcgcgtcgtc attcaccggt ccgccgtccg acaggggacg
     3481 gcgacatgag cgtcgcgacc ggccaggtga gcgcgccgcc gaacgatctg tggaagatcc
     3541 gcggacgccg cgcgatcgtg aaccggctga tgaccggcgt catgtatctg gccttcgtcg
     3601 tcgccgtcgt cccgcttgcg ctggtcatct ggtacaccgt cggcaaggga ctgacgcggt
     3661 tcagcttcaa cttcctgacg cactcgatgg ccggcgtcgg acccgacgac atcggcggcg
     3721 gcatttccca cgccatcgtt ggtaccgtcg agcagctcgc gatcgcctcg gtgatctcgg
     3781 tgccgcttgg cctgctggtc gcgatccacc tcaacgagta cggccaaaga ggcttctcga
     3841 ctgtggtgcg gttgctggtc gacgtcatga ccggcatccc gtccatcgtc gccggcctgt
     3901 tcatcttggc cttctgggtg atcgggctgc accagggtta ctcgggtttc gccggctcga
     3961 tggcgctggc gatcctgatg ttgccggtgg tcatccggtc atcggaagaa atgctcaagc
     4021 tggtgccgaa ctcacttcgc gaggcggcct atgccctcgg catcccgaag tggcgggcca
     4081 tcctgttcat catcctgccg accgctcgca ccggcatcac gaccggcgtc atgctcgcga
     4141 tcgcccgggt gacaggcgaa accgcgcctc tgctgctcac agcgtttggc acccttttcc
     4201 tcaacaagaa tccgacacac ggcccgcagg aagcgttgcc gctctacgta ttcagctcgg
     4261 ccgcggctgg cacaccagct gacgtgaatc gtgcctgggc tgccgcgatg acccttatcc
     4321 tgatagtcgt gctgctctat gtgatcgcgc ggctgcttac gcgccgagac gtactcaacc
     4381 ggaggtgagc ggtcccatgg ccaagcgcat cgaagcgcta ggtctgtcgt gctactacgg
     4441 caaccacaag gctgtcgaga acgtcacgat gacgatcgaa cccaaggcgg tcaccgcctt
     4501 gatcggtcct tcgggttgtg gcaaatcgac gttcctgcgc agtctcaacc ggctgcacga
     4561 ggtcattccc ggcgcgcaca tcaccggtca ggtgttgctc gacgaccgcg acatttacgc
     4621 gcctggcgtc gatcccgtcg cgcttcgtcg cagcgtcggc atggtgttcc agcggccgaa
     4681 cccgtttcca actatgtcga tctacgataa cgtcgccgcg ggcctgaaat tgggcggcag
     4741 caagggaaaa gacctcgaca cggtggtcga gcgcgcactg acaggtgctc acctttgggc
     4801 tgaggtaaag gaccggctca agaagccggc cctcgcactg agcggcggcc agcagcagcg
     4861 actgtgcatc gcgcgcgcca tcgccgtcga gccagaagtg ctgctgatgg acgaaccatg
     4921 ctcggccctc gacccgatct cgacgctggc gatcgaagac ctcatcgccg aactcaagga
     4981 ccgctacacg atcgtcatcg tgacccacaa catgcagcag gcgagtcggg tcagcgagac
     5041 gactgggttc ttcacgctgg ccggcgccgg gcaaccaggt gtgctggtcg agtacgacgc
     5101 gacgagccgg atcttctcgg tgccctccga caagcgcacc gaggactaca tcaccggccg
     5161 caccggctga tccaccgcaa gccctttcct ggttgctgca gagggtctgt ctagcccgag
     5221 cctccggaat cccggtctgg ttctggacgc ggctgcgatt gctgtgaggg cggcgggggt
     5281 gccgggatga cgacgacatc gggctcggac ggaccgaaca ttgattccca ctgttcgggg
     5341 gacggcggct tgttgttatc gcgtatgacc tgccggtcct cgcggccggg gcgggtcagg
     5401 aacaagtacg tcgcggcgcc caggaagacg aggatggacg tccagtcgtt gagtcgaagg
     5461 cccgcgatgt ggttggcgtg gtccactcgc aatgattcga cccacccgcg cccagcggtg
     5521 taggcgagca cgtagagggc gaacaagcgg ccgcggcgca acgcgagccg ccgatcggcc
     5581 cagaccagga gtagcgcgac gccgacgtcc cacagcgact catacaggaa ggtcggctgg
     5641 tacagcgcga ccgtcggggt ggacggtgga cggttcgccg gatcaatgcg caacgcccac
     5701 ggcaggccgg aggggcgccc gtagagctct tggttgaagt agttccccca ccgtccgatc
     5761 ccttgggcga gcaccaagcc tggggcgagt gcgtcagcga agtcccggag cggtacccct
     5821 cgtcggcggc atccgatcca cgccccgacc gcgccgagtg caaccgcacc ccagatgcca
     5881 aggccgccgc gccagatgta gagcgcctgg atcgggtgct cgcccttctt gaagtacaac
     5941 tccgggtcgg tgatcacgtg gtacagccgg ccgccgatga tgccgaacgg caccgcccac
     6001 atcgcgatcg cggacacgtc gtcggggtgg tgtccgcggg cggcccagcg gcgacgggtc
     6061 acgaaaagcg cgacgaagat gccggcgatc aggcatagcg cgtacgcgcg gatcggtacc
     6121 ggccccagat gccacacccc ctgcgaggga ctgggcaggt aatcccgcat cgggtgtcgg
     6181 tctccgtcca gttggctgca aaacaaagta ccccggttac cgaacatttc ggtaggcggc
     6241 ccttcccgcg tagagcgcta gctgtactcc gcgaccacgg cgtcggagaa caccggccac
     6301 gcggaagcag cccagggccc gaacggggtg tccgcgagcg acacgcagcc caggcctagc
     6361 gatgggtcga tccacaggaa acttcccgac ccaccgaaat gcccaaatgt ggacggcgag
     6421 ttgttccgtc cggtccagtg cgggtccttt ccgtcccgga tctcaaaccc caacccccag
     6481 tcgttgggag cgcaccgccc gaaccccggc aggacgccat cgagaccggg aaaggcgacc
     6541 gccgtcgcga gggccagcgt cgccggcgcg accagagtgg gcgccagaag ttcgcgggcg
     6601 aacctcaaca ggtcgaccag cggcccgacg ccgcccgccg acggcgtccc ctcgatgctt
     6661 gaatcgtgca tccctagggg cccgaacacc gcttcggcgg cgtatcgcgc gaacggcaca
     6721 ccggtatgcg agctcaccaa cgcgccgagc aaatcgaagc cggcgttgga gtagacgcgt
     6781 cggcgttccg gcttcgaaat cgccgcgccc tcgtcgacca gccgcaggac gcacaccgcc
     6841 gtgacgagtt tggtgaccga cgcccactcg tggcgccgcg ccacgtcacc ggtctcgccg
     6901 agtttccggg tcgcgtcggt aatgccggca gccgggttgg acgccgacca gtgggcgact
     6961 tgcgcaaggg cggtcccggg cggcggcaca ccatgagcct agggcgacgc cgccggcagc
     7021 ttcagctcat cgatcagcgc gagaacgcgc cgctccacct cgtcgcggat agggcgaacc
     7081 gcggcgacgt cctgtcctgc cgggtcgtca agaatccact cctcgtagcg cttccctggg
     7141 aacaccgggc acgcgtcgcc gcatcccata gtgatgacga
//
LOCUS       DATCBJ010000191         2692 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_278962, whole genome shotgun sequence.
ACCESSION   DATCBJ010000191 DATCBJ010000000
VERSION     DATCBJ010000191.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2692)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2692)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2692
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_278962"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1318
                     /locus_tag="VII50_03910"
     CDS             <1..1318
                     /locus_tag="VII50_03910"
                     /inference="COORDINATES: protein motif:HMM:NF012855.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004198 - calcium-dependent cysteine-type
                     endopeptidase activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="C2 family cysteine protease"
                     /protein_id="HEY5050022.1"
                     /translation="QLLDMPLPKIVDPKHGSPIYAGVPLHFGAGGGETPVDPSVDLRR
                     VFETTPRATELLHDDLLSDATLRGDGTSIHQLSAQTADTLGAVLRKVSTAPVQVADAM
                     HTQRAAEVATATRLSELSSATFVEAGKSLGQLNRDYLILPPSWLKQVLSDLDFGMRGE
                     FFVDGPTATAPVQGALGDCWLIAAMAAVAWTHPELVSERTRRENIPGNVDAGSADLYF
                     EFTDQLTIPVLFFTISIPFVFKLWIGEELPQTADGGWIYARSSVAGEIWPAEIEKAAA
                     VWRSGGNADDPRSSDYTHLAGGDPAWAVHILTGGTPWYHWADADDTWSTIRSHCSGQR
                     ATDPLVAWTWGSSDDSPDKVDYGDAQIAANHAYSILGTWETNNRQYVILRNPWGWYEA
                     TLNSYAGTWTTPESWGNADTVLPGNGVFALEIHTFREYYMGFGGAS"
     gene            complement(1320..2438)
                     /gene="dapC"
                     /locus_tag="VII50_03915"
     CDS             complement(1320..2438)
                     /gene="dapC"
                     /locus_tag="VII50_03915"
                     /EC_number="2.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002515121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinyldiaminopimelate transaminase"
                     /protein_id="HEY5050023.1"
                     /translation="MSGRAGGRGRELPDFPWDSLAPYVAMARAHDGGVVDLSVGTPVD
                     PVPVVVQDALAATTNTPGYPATYGTSALREAAAGWMRRRHGVTGADPANVLPTIGAKE
                     FVAWLPTLLGLGTGDTVGIPALAYPTYDIGARLAGAQPLVVDGTVGVGPAPLAMLWVN
                     SPANPHGQVLGVEHLRKIVEWARDRGTIVVSDECYLEFGWDDEVAALSVLHPDVCGGS
                     HAGLLAVHSLSKRSNLAGYRAGFVTGDGELIAELLEVRKHAGMIVPAPVQAAMVAALD
                     DDAHVDAQRARYLARRAILWPALVSAGFSVEHSQGGLYLWATRDEDCWASIEWLAQLG
                     ILAGPGIFYGEAGRRHVRIALTATGERVAEAARRLTSA"
     gene            complement(2435..>2692)
                     /gene="fdxA"
                     /locus_tag="VII50_03920"
     CDS             complement(2435..>2692)
                     /gene="fdxA"
                     /locus_tag="VII50_03920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006214165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HEY5050024.1"
                     /translation="CIYEGGRMLYIHPDECVDCGACEPVCPVEAIFYEDDVPDKWKEY
                     YKANVDFFDDLGSPGGASKLGPVTKDHPLIAALPPQEQETH"
BASE COUNT          476 a          937 c          883 g          396 t
ORIGIN      
        1 tcagttgctc gacatgccgc tgcccaagat cgtcgacccg aagcacggct cgcccatcta
       61 cgccggcgtg ccgctgcact tcggcgcagg aggaggcgag acgcccgtcg acccgtcggt
      121 cgacttgcgc agggtgttcg aaacgacgcc gcgtgcgacc gaactactgc acgacgatct
      181 gctcagtgac gcgacgctca ggggcgacgg cacgagcatc caccagctgt cagcgcaaac
      241 cgccgacacc ctcggcgctg tgctgcgcaa ggtgtcaaca gccccggtgc aagtggccga
      301 cgcgatgcac acccagcgcg ccgcggaggt cgctacggcg acgagactgt ccgaactttc
      361 gtcggccacc ttcgtcgagg ccggcaagtc actcggtcaa ctgaatcgcg actacctcat
      421 cctgccgccg agctggctca agcaagtgct gtccgacctc gacttcggca tgcgcggtga
      481 gttcttcgtc gacgggccga cagcaaccgc gccggtgcag ggtgcactcg gtgactgctg
      541 gctcatcgca gcgatggcgg cggttgcgtg gacccacccg gagttggtct ccgagcgcac
      601 ccgccgggaa aacatcccag gcaacgtcga tgccggaagc gccgatctgt acttcgagtt
      661 caccgaccag ctgacgatcc cggtgctgtt cttcactatc tcgatcccgt tcgttttcaa
      721 gctgtggatc ggcgaggagt taccgcagac ggccgatggc ggctggatct acgcgcggtc
      781 gtcggtggcc ggcgagatct ggccggccga gatcgagaag gcagcagcgg tgtggcgcag
      841 cggcgggaac gccgacgacc cgcgcagcag tgactacacc cacctcgccg gcggcgatcc
      901 cgcgtgggcg gtgcacatct tgaccggggg aaccccctgg taccactggg ctgacgccga
      961 tgacacctgg tcgacgatcc gctcgcactg ctccggccaa cgcgcgacag acccgctcgt
     1021 ggcctggaca tggggcagca gcgacgactc ccccgacaag gtcgactacg gcgacgcgca
     1081 gatcgcggcg aaccacgcct actcgatcct cggcacgtgg gagaccaaca accgccagta
     1141 cgtgatcctg cgcaacccgt ggggttggta tgaggcgacg ctgaacagct acgcaggtac
     1201 atggacaacg ccggagagct ggggtaatgc cgacaccgtg ttgcccggca acggtgtctt
     1261 cgcactcgag atccacacgt tccgcgagta ctacatggga ttcggcggcg cgtcttagtt
     1321 cacgcggagg tcaaccggcg agcggcttcg gcgacgcgct cgccggttgc ggtaagggcg
     1381 atccgcacgt gccggcgtcc ggcctcgccg tagaagatgc ccgggcccgc cagaatcccg
     1441 agctgcgcga gccactcgat actggcccag cagtcctcgt cgcgggtcgc ccacaggtac
     1501 agcccgccct gcgaatgctc gaccgagaag ccggcggaaa ccaatgccgg ccacaaaatt
     1561 gcgcgccgcg cgagatagcg cgcgcgttgc gcatcgacat gcgcatcgtc gtccagcgcg
     1621 gccaccatcg cggcctgcac aggcgcgggc acgatcatcc cggcgtgctt gcggacctca
     1681 agtagctcgg cgatcagctc accgtcgccg gtgacgaagc cagcccggta cccggcgagg
     1741 ttcgaacgtt tcgacaagga gtggacggcg agtagccccg catgtgagcc accacagaca
     1801 tcgggatgca gaaccgacaa cgccgccacc tcgtcgtccc agccgaactc gaggtagcac
     1861 tcgtcggaca ccacgatcgt gccgcgatca cgcgcccact cgacgatctt tcgcaggtgc
     1921 tcgacgccaa gaacctgacc gtgcgggttg gcgggcgagt tcacccacag catcgccagc
     1981 ggcgcgggcc cgacaccgac cgtgccgtcg acgaccaacg gttgcgcgcc ggccagccgg
     2041 gcgccgatat cgtaggtcgg gtacgcgagt gcggggatgc ccacggtgtc gcccgttccc
     2101 aggccgagca acgtcggcag ccaggcgacg aactccttgg cgccgatggt cggcagcacg
     2161 ttcgcggggt cggcgccagt taccccgtgc cggcggcgca tccagccggc agcggcctcg
     2221 cgcagcgcgg acgtgccgta ggtcgccgga tagccgggag tgttcgtagt cgccgccaac
     2281 gcgtcctgca cgacgacagg gaccggatca accggcgtcc cgactgaaag gtcgacgacg
     2341 ccgccatcat gggcgcgcgc catggcgaca tagggcgcga ggctgtccca cggaaaatca
     2401 ggcagctccc tgccacgccc acccgcacga ccgctcagtg cgtctcctgc tcctgcggcg
     2461 gcaacgcggc gatcagcggg tggtccttcg tgaccgggcc gagcttggac gcgccaccgg
     2521 gcgagccgag gtcgtcgaag aagtcgacgt tcgccttgta gtactccttc cacttatccg
     2581 ggacgtcgtc ctcgtagaag atcgcctcga ccgggcacac cggctcgcag gcgccgcagt
     2641 cgacgcactc gtcggggtgg atgtagagca tccggccccc ctcgtagatg ca
//
LOCUS       DATCBJ010000192         4445 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_228910, whole genome shotgun sequence.
ACCESSION   DATCBJ010000192 DATCBJ010000000
VERSION     DATCBJ010000192.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 4445)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4445)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4445
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_228910"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..230)
                     /locus_tag="VII50_03925"
     CDS             complement(<1..230)
                     /locus_tag="VII50_03925"
                     /inference="COORDINATES: protein motif:HMM:NF017072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)(+) transhydrogenase (Re/Si-specific)
                     subunit alpha"
                     /protein_id="HEY5050025.1"
                     /translation="MKVVVPRETAQYERRVACVPDTVTRLLAGGLSVSVERGAGLAAG
                     YSDEAYVSAGAQIADRDALKAADVVLAVQPLPL"
     gene            complement(382..1098)
                     /locus_tag="VII50_03930"
     CDS             complement(382..1098)
                     /locus_tag="VII50_03930"
                     /inference="COORDINATES: protein motif:HMM:NF022655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2993 domain-containing protein"
                     /protein_id="HEY5050026.1"
                     /translation="MTRAVRVFIVLAILAGIGFAADRYAAHVAAGQIASAVQTDAKLA
                     RKPHVTIKGFPFLTQVVSGNYKHIDVTATDIFQQGEPQGSVLKLDFAGVRITISKALS
                     GHVGTVPVRSVTGTVAIPFADLLAVADIPGLTIISALPGGNQIKVSEVVAAGPTKVTA
                     LVTAGVTVRGGSLVLSALDVTQANGKPVPAAVRQEVLAQAVFSVKLPGLPTGVQMTAV
                     SVTAGGLLVTLHADALVLTH"
     gene            complement(1132..1941)
                     /locus_tag="VII50_03935"
     CDS             complement(1132..1941)
                     /locus_tag="VII50_03935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5050027.1"
                     /translation="MTSVPSVPPGPPVSAAPSGSSTRAVLIAAFAGLSTSLFAVVVLA
                     ASSSGRWLLAAALLVVQLALVIGWFRSARLTTVGQASGAFAAIAAAVAADVVLLRTHD
                     RANVRALAGVLAGLVILGFAIQLARRDGRERLTDAMAASVAAGALAIAVSVLLAVRGG
                     PHGTTVVAVALAGVAVGVLPVQPQVPLWASIPAGLALGAGAGLLVSHETGAFGIGTGA
                     VVALVAVVLAVGARAALAKVPPAVVAWPVTATLPLVVVPPAVLVVARIMVG"
     gene            complement(1938..2327)
                     /locus_tag="VII50_03940"
     CDS             complement(1938..2327)
                     /locus_tag="VII50_03940"
                     /inference="COORDINATES: protein motif:HMM:NF014636.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HEY5050028.1"
                     /translation="MFPSGFGDVNAGNRGVSDPPTGLIRYWAAAREAAGCSEEPYVAE
                     TLEQALAAATAAHGADLARVLARCAFVVDGTPVSRRDPATVRLAAGGTIEVLPPFAGG
                     AGPALGDRVAARQPSEVLSRWGATMRG"
     gene            2450..3241
                     /locus_tag="VII50_03945"
     CDS             2450..3241
                     /locus_tag="VII50_03945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEY5050029.1"
                     /translation="MSTLLLLTNALQPSSEVLPALGLLLHKVRVAPAEVSALLDAPPA
                     DAILVDGRRDLAHARSLCQLLRTTGVSCPLFLVMTEGGLSVVTAEWGIDDVLLDSTGP
                     AEVEARLRLAAGRLELAARNEDADDEVIRSGELTIDEATYSARLKGRSLDLTFKEFEL
                     LKYLAQHPGRVFTRAQLLQEVWGYDYFGGTRTVDVHVRRLRAKLGTEHEALIGTVRNV
                     GYKFVLPQVERSAAHSDAVDDQGDDDDGAEIVMHDLRLRDVSIRD"
     gene            3320..4303
                     /gene="mshD"
                     /locus_tag="VII50_03950"
     CDS             3320..4303
                     /gene="mshD"
                     /locus_tag="VII50_03950"
                     /EC_number="2.3.1.189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035447 - mycothiol synthase activity
                     [Evidence IEA];
                     GO_process: GO:0010125 - mycothiol biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mycothiol synthase"
                     /protein_id="HEY5050030.1"
                     /translation="MDTPTRVDVVGRLTPDQIEAVTEIVDDATDADGVRPLSEHVMLH
                     LRYGGDEPARNLLLWQGSHLVAYAHLDTTDPVDGPSAEMVVAPATRRQGFGRTIIAAA
                     LEHAHGPLRLWAHGGLAEATLMAEHLGFQRIRVLLQLRRSLFASLPPVVLPDGVTVRT
                     FQPGADDEAWLALNARAFASHPEQGLWTLEDLRQRIAEQWFDPAGFFLAEKQIDGHPQ
                     LVGFHWTKVHGHHLHEEHLVHTHGSKPSHSHPEEHGHEPIGEVYVVGVDPSAQGMGLG
                     RRLTLIGLQHLRSLGLQQVMLYVDESNVAAIRLYESLGFSRWDTDVLFRHE"
BASE COUNT          771 a         1582 c         1437 g          655 t
ORIGIN      
        1 agcgggagcg gttgcaccgc aagaaccacg tcggccgcct tcagcgcgtc acgatcagcg
       61 atctgcgcac cagcggagac atacgcctcg tcgctgtagc cggcggcaag cccggcgccc
      121 cgctcgacgg acacgctcaa accgccagcc agcagtctcg tgacagtgtc cggaacacac
      181 gcgacccggc gctcatactg tgccgtctcc cgcggcacca ccactttcac gcccgaaacc
      241 taccccacgc gatcacgcga ccggacctaa acgaggcccg ggtgtccgat cggcgcctga
      301 gtcccgccga gtgttgtccg tgacgctgcc cgcgtcgcca cctaacgcaa gccccccgcg
      361 ttccgccgcc agcagcccga gtcagtgggt cagcacgagg gcgtcggcgt gcagcgtcac
      421 cagcagccca ccggcggtca cgctcaccgc agtcatctgg acgccggtcg gcaggccggg
      481 gagcttcacc gagaacaccg cttgggccag tacctcctgc cggaccgccg cgggtaccgg
      541 cttcccgttg gcctgcgtca cgtcgagcgc gctcagcacg agtgaaccgc cgcgcacagt
      601 cacgccagcc gtcaccagcg ccgtcacttt cgtcggcccg gccgccacga cctcgctgac
      661 cttgatctgg ttgcctcccg gcagcgccga aatgatcgtc aggcccggga tgtccgcgac
      721 cgccagcagg tcagcgaacg gaatcgcgac ggtgcccgtg accgagcgca cgggcaccgt
      781 gccgacgtgc ccgctcaggg ctttcgaaat cgtgatccgc acgcctgcga agtcgagctt
      841 gagcaccgat ccttgcggct ctccctgctg gaagatgtcg gtcgcggtca catcgatgtg
      901 cttgtagttg ccgctcacca cctgcgtgag gaaggggaaa cccttgatgg tgacgtgcgg
      961 ctttcgagcc agtttcgcgt cggtctggac ggcggaagcg atctggccgg ccgcgacatg
     1021 ggcggcgtac cggtcggcgg caaacccgat ccccgcgagg atcgccagta cgatgaagac
     1081 ccgaacggcc cgcgtcatcc gcgagagccg caacgcagga atcgcacgcg gttaaccgac
     1141 catgatgcgc gcgacgacca gcacggcagg ggggacgacg accagcggca gggtggcagt
     1201 gaccggccag gccaccacgg caggcggaac cttcgccagc gcagcccgcg cgcccaccgc
     1261 gagcacgacc gcaaccagcg caacaaccgc gccggtaccg atcccgaacg cgcctgtttc
     1321 gtggctgacc agcagcccgg caccagcccc aagcgcaagg ccggccggaa tcgatgccca
     1381 cagcggcacc tgcggttgga ccggcaagac gccgaccgcg actccggcca gcgcgaccgc
     1441 caccacggtc gtgccgtgcg gcccgccgcg cacggcaagc aacaccgaca cggcgatcgc
     1501 cagcgcaccg gccgcaaccg acgctgccat ggcgtcggtc agccgctcgc gaccatcgcg
     1561 ccgagctagc tgaatcgcga acccaaggat cacgaggccg gcgagcactc cagccagcgc
     1621 gcgcacattg gcccgatcat gcgtgcgaag caagacgacg tcggccgcca cggctgcggc
     1681 aatcgcggca aaggccccgc tggcctggcc gacggtcgtc aggcgcgcgc tgcgaaacca
     1741 tccaatgaca agcgcgagtt ggacgacgag aagtgccgcc gccagcaacc accggccact
     1801 cgacgaagcg gctaggacaa ccacggcgaa cagcgacgtc gagaggccag cgaaagcagc
     1861 gatcagcact gcccgggtag acgacccgga cggcgccgcc gaaacgggcg gcccaggcgg
     1921 cactgacggc acggaggtca ccctcgcatg gtcgcacccc atcttgagag aacttcggac
     1981 ggctgccgcg cggccacccg gtccccaagc gcaggacccg caccgccggc gaagggcggc
     2041 aggacttcga tcgtgccacc cgccgcgagc cgcacggttg ccggatcacg cctgctcacc
     2101 ggcgtcccat cgacgacgaa tgcgcacctc gcaagcaccc gcgccaagtc cgcgccgtgc
     2161 gcagcggtgg ccgcggcgag cgcctgttcg agggtttccg cgacgtacgg ctcctccgaa
     2221 caaccggccg cctcgcgcgc agccgcccag taccggatca gcccggtcgg ggggtccgaa
     2281 acgccgcggt taccggcgtt cacgtcgccg aaaccagatg gaaacactca cactcgtatc
     2341 cttggctcag ttcgctggct agcggaacgc gtcccttggc ccgcgacgcc ctcagcgtcg
     2401 aggggtgtgg cacccattct ggcgaagttt ttcgtgaggg gaggccggcg tgagcaccct
     2461 gctgttgctg accaacgcct tgcagccgtc atctgaggtc ctgcccgccc ttggtctgct
     2521 cctgcacaaa gtgcgggtcg cacccgctga ggtgtcggcc ctgctcgacg ctcccccggc
     2581 tgacgccatc ctcgtcgatg gccgccgcga cctggcccac gcccgatcgc tatgccagct
     2641 gctgcggacg accggagtgt cctgcccgct gttcctcgtc atgaccgagg gtggtctgtc
     2701 cgtggtgacg gccgagtggg gaatcgacga cgtcctgctc gactcgaccg gcccggccga
     2761 ggtcgaggcg cggctgcggt tggccgccgg ccgcctcgag ttggccgccc gcaacgagga
     2821 cgccgacgac gaggtcatcc gctcgggtga gctgactatc gacgaagcga cctactcggc
     2881 ccgcctcaag ggccgctcgc ttgacctgac gttcaaggaa ttcgagctgc tgaagtacct
     2941 tgcgcagcac ccagggcggg tcttcacccg ggcccaactg ctgcaagagg tctggggtta
     3001 cgactatttc ggcggcacca ggaccgtcga cgtccacgtt cggcgcctgc gagccaagct
     3061 cggcaccgag cacgaagcgc tgattggcac cgtccgcaac gtcggttaca agtttgtgct
     3121 gccgcaggtc gaacgctcgg ctgcgcacag cgatgccgtc gacgaccagg gcgacgacga
     3181 tgacggcgcc gagatcgtga tgcacgacct gcgcttgcgg gacgtcagca tccgcgacta
     3241 ggtcctcgcg ccgccgcggc taggttttct gcgcgtcggc ggttaggtct ggcgcgctcg
     3301 gtcacctatg gtcagcggcg tggacacgcc tacccgcgtt gacgtcgtcg gccggttgac
     3361 gccagaccag atcgaggccg tcaccgagat cgtcgacgac gcgaccgatg ctgacggcgt
     3421 ccgaccgttg tccgaacacg tgatgctgca cctgcgctac ggcggcgacg agccggcccg
     3481 caatttgttg ctgtggcaag gatcgcacct cgttgcctac gcccacctcg acacgaccga
     3541 tccggtcgac ggtccgagtg cggagatggt cgtcgcgccg gcaactcgcc ggcagggctt
     3601 cggtcgcacc atcatcgccg cggcactcga gcacgctcac ggcccgttgc ggttgtgggc
     3661 gcacggtgga ctcgcggaag ccaccttgat ggcggagcac ttgggctttc aacgcattcg
     3721 cgtactgctg caacttcgcc gatcgctttt cgcgtcgctg cctcccgtcg tcctgcccga
     3781 cggcgtcacg gttcgaacat ttcaaccagg cgcggacgac gaggcgtggc tcgccctgaa
     3841 tgcgcgcgcg ttcgcgtcgc atccggaaca agggctctgg acgctagagg atctgcggca
     3901 gcgaatcgcc gagcaatggt tcgacccggc cggatttttc cttgcagaaa agcaaatcga
     3961 tggccatccg cagcttgtcg gattccattg gacgaaggtg catgggcatc acttgcacga
     4021 ggagcacctc gtgcacacgc atgggtcgaa gccttcgcat tcgcacccgg aggagcacgg
     4081 gcacgaaccg atcggcgagg tttacgtcgt cggcgtggat ccgtcggcac aaggcatggg
     4141 actcggccgc cggctgacac tcatcggcct acagcacctg cggtcgctcg gcttgcagca
     4201 ggtgatgctc tacgtcgacg aatcgaacgt cgcggcgatc cggctctacg agtcactcgg
     4261 tttcagccgc tgggacacag acgtgctgtt ccggcacgag tagcctctag ctcacaaggg
     4321 cgaccaagcc gagggcccga tcgatggcat cggcagtcag tttcaccgcg ctcgcggccg
     4381 cgacgatgat gagctcgctg tagaggtcga tctcttccag aaacgctgag tcgtgaatgc
     4441 gcggg
//
LOCUS       DATCBJ010000193         2388 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_408165, whole genome shotgun sequence.
ACCESSION   DATCBJ010000193 DATCBJ010000000
VERSION     DATCBJ010000193.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2388)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2388)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2388
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_408165"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..612
                     /locus_tag="VII50_03955"
     CDS             <1..612
                     /locus_tag="VII50_03955"
                     /inference="COORDINATES: protein motif:HMM:NF019119.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HEY5050031.1"
                     /translation="VSWVRDRIIQLRARAEYSPRLHFDVYGTIGEAFGGDINAVADYL
                     VRLGAAGQPFAVTVEHVVDAGSRDAQVRAFAALRASLRDRGCDVRIAVDEWCNTLEDI
                     EVFVRAQAADVIHVKTPDLGGINNTIDALLLVARHGLLAYCGGSCNETDRSAQVSAHI
                     AMACGATQVLAKPGMGVDEGMMIVGNEMARVAALVAGRGRAPA"
     gene            609..1811
                     /locus_tag="VII50_03960"
     CDS             609..1811
                     /locus_tag="VII50_03960"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009086396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEY5050032.1"
                     /translation="MKPLEDVRIIAIEQYAAGPFGSVHLADLGADIIKIEDPATGGDI
                     GRYVPPLQEEEDSLFFETFNRNKRSIGIDLKTPAGRAVFEDLVRVSDVVYSNLRGDVP
                     QHLRITYADLADVNPRIVCCALTGFGMTGPRRDEPGYDYFLQGITGWMDLTGEPSGPP
                     TKSGLSLVDYSAGLVAAIAMLTGIHAARRDGVGMDCDLSLFDTALAMLTYPAIWHLNG
                     DFTPARTHHSAHPSLVPFQAFQTSDAWIVVGTPKEKFWVRLLNAVGLPELASDPRFTT
                     FANRRSNADALLPMLERVFASRPASEWLEVLRRAGVPCSPVNTVAQALRDEQTAARGM
                     VVDTEHPRFGTVRQLRSPVRVGAGQPEYRRAPLRDEHQDQVLRELLGYDDTRIAQLRA
                     SGAFGPAA"
     gene            1851..>2388
                     /locus_tag="VII50_03965"
     CDS             1851..>2388
                     /locus_tag="VII50_03965"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY5050033.1"
                     /translation="MVSESGRVDGTFVGRRSELASLLTQAADVRGGHPRVVLLTGEPG
                     IGKTALAHRFVSQAQGFQLWEASGEEAEQLLTFGVIEQLVRAVPESVSPALRNLGHQR
                     GETQDPIWVGAALLELLGTMQAAGPVLLLIDDAQWADRPSLQALVFALRRLQADRVLA
                     LMVSRSGSARGHLAGFHRL"
BASE COUNT          402 a          773 c          810 g          403 t
ORIGIN      
        1 gtctcgtggg tgcgcgaccg gatcatccag ctccgcgccc gagccgagta ctcgccacga
       61 ttgcacttcg acgtgtacgg gacaatcggt gaggcgttcg gcggcgatat caacgcggta
      121 gcggactatc tcgtgcgtct cggcgcagca ggccagccct tcgcggtcac cgtggagcat
      181 gtcgtcgacg cggggagccg ggatgcgcag gtgcgagcat tcgcagccct gcgcgcgagc
      241 cttcgcgatc gtggctgcga cgtgcgaatc gccgtcgatg aatggtgcaa cacgctcgag
      301 gacatcgagg tgttcgtaag ggctcaggcg gccgacgtca tccacgtcaa gacgcccgac
      361 ctcggtggga tcaacaacac gatcgacgcg ctgctgctgg tcgcgcgtca cggtctcctc
      421 gcatattgcg gcggcagctg caatgagaca gatcggtccg cgcaggtgtc ggcgcacatc
      481 gcaatggcct gtggcgcaac ccaggtactc gccaagcccg ggatgggtgt ggacgaggga
      541 atgatgatcg tcggcaacga gatggcgcgc gttgccgcac tggttgccgg tcgcggccgt
      601 gcaccggcgt gaaacccctc gaggacgtgc gaatcatcgc catcgagcag tacgcggctg
      661 ggccgttcgg gagcgtgcac ctcgccgacc ttggcgccga catcatcaag atcgaagacc
      721 cggcaaccgg tggcgatatc ggtcgctacg ttccgccgtt gcaggaggaa gaggacagcc
      781 tgttcttcga gacgttcaac cgcaacaagc gaagcatcgg tatcgacctc aagacgcccg
      841 caggacgtgc ggtgttcgaa gatctcgtgc gcgtcagcga tgtcgtgtac tcgaatcttc
      901 gcggcgacgt tccgcagcac ctacgaatca cctacgccga tctcgccgac gtcaatccgc
      961 gcatcgtctg ctgcgcgctg accggcttcg gtatgacggg accgcgcagg gacgagccgg
     1021 ggtacgacta cttcctccag gggatcaccg ggtggatgga tctcacgggc gaaccgtcgg
     1081 gcccgcccac caagtccggg ctgtcactgg tcgactactc ggccgggctg gttgccgcaa
     1141 tcgcgatgct caccggcatc cacgcggcac gacgcgacgg cgtggggatg gactgtgacc
     1201 tcagcctctt cgataccgcg ctggcgatgc tcacgtaccc cgcgatctgg cacctcaacg
     1261 gcgatttcac gcccgcgcgc acgcatcact cggcgcatcc gtccctggtt cccttccagg
     1321 cgttccagac cagcgacgca tggatcgttg tcggcacccc gaaggagaag ttctgggtgc
     1381 ggctgctgaa cgccgtcggg ctccccgagc ttgcgagcga cccgcgattc accacattcg
     1441 cgaatcggcg cagcaacgct gatgccttgc tcccgatgct cgagcgtgtg ttcgcgtcgc
     1501 gtcccgcgtc ggaatggctc gaagtcctga ggcgcgctgg ggttccgtgc agccccgtca
     1561 acaccgttgc ccaggcgctg cgcgacgagc agaccgctgc ccgcggcatg gtcgtcgata
     1621 ccgagcaccc gcgcttcggc acggtcagac aactgcgcag cccggttcgg gtcggcgcgg
     1681 ggcaacccga gtaccggcga gcaccgctcc gcgacgaaca ccaggaccaa gtgcttcgag
     1741 agcttctcgg ctatgacgac acccgcatcg cgcagttgcg cgcaagcggc gccttcgggc
     1801 cggcggcgta acaaaaaccg aggtgacatc ggcggtagga tctgtcgcca atggtcagcg
     1861 agagcggacg ggtggacgga accttcgtcg gcagaaggag tgagctcgcg agcctgctga
     1921 cacaggccgc cgatgtgcgg ggtggccatc cgcgcgtggt actgctcact ggcgaaccgg
     1981 ggatcgggaa gaccgcgctc gcgcaccgtt tcgtcagcca ggcacaggga tttcagctct
     2041 gggaggcgag tggggaggag gccgagcagc tgctcacctt cggggttatc gaacagcttg
     2101 ttcgtgcagt tccggagtcg gtgagccctg cgctccggaa tctcgggcat caacgcggtg
     2161 agacccaaga cccgatctgg gtcggcgctg ccttgctcga gttgctgggc acgatgcagg
     2221 ccgcggggcc ggtgctgctg ctgatagacg acgcgcagtg ggccgatcgt ccgtcgctgc
     2281 aggcgctggt gttcgcactc cgccggctcc aggcagaccg tgtcctcgcg ctgatggtgt
     2341 cgcggagtgg atcggcgcgc ggccaccttg ccggattcca ccggctgg
//
LOCUS       DATCBJ010000194         2075 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_228539, whole genome shotgun sequence.
ACCESSION   DATCBJ010000194 DATCBJ010000000
VERSION     DATCBJ010000194.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2075)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2075)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2075
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_228539"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(39..1307)
                     /locus_tag="VII50_03970"
     CDS             complement(39..1307)
                     /locus_tag="VII50_03970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HEY5050034.1"
                     /translation="MKAEIVAIGTELLLGDIVNGNAAWLGRELADIGIDVDLTTAVGD
                     NIGRISAAVGRACERADVVVLTGGLGPTQDDLTREALAELAGVRLVRDAQLAAALRAR
                     YVASGRIDFPENNLRMTDRPEGASALPNPAGTAPGLRLTIGSRGTTVYALPGVPQEMR
                     EIFSTWMRPELVERAGPGTIVLSRRLHTVGVWESQVSQALAEIDAALAAAANPTIAYL
                     ASQGQTLVRMTAKAATETAARELIAGVETRVRAALGDVIYGVDDDTLESVVHQLLLTA
                     GATVATAESLTGGLLGAALSSTPGASETYRGGAVLYATDSKAAVVDVPATLLAERGPV
                     DPDVAVAMADGARRAFDATYGLGTTGVAGPTEQDGKPVGTVYIALVGPADGPMVRELR
                     LAGGRDRVRQGSVLTALDVVRRHLSGLPQR"
     gene            complement(1304..1918)
                     /gene="pgsA"
                     /locus_tag="VII50_03975"
     CDS             complement(1304..1918)
                     /gene="pgsA"
                     /locus_tag="VII50_03975"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HEY5050035.1"
                     /translation="MTEITESATTPIPQAGVRNLANALTVARLVMVPIFVALLLSARG
                     EHDGWRLAATAVFVLASLTDRFDGEVARRRGTITDFGKIADPIADKALTGAALISLSS
                     ISDLPWWVTVVILAREIGITLLRFWVIRRGVLAASRGGKVKTLVQVVAIGLYLLPLTG
                     PMASLRAYLMGVAVVITLVTGVDYVARAIRLRRATSSRAASAAL"
BASE COUNT          340 a          754 c          691 g          290 t
ORIGIN      
        1 aaccggcacc tggctagcct ttgcggcgga gccgcccgtc aacgctgcgg cagcccggaa
       61 aggtgccggc gtacgacgtc gagcgcggtg aggacgctcc cctgccgcac ccggtcgcgt
      121 cccccggcca ggcgcagttc gcgcaccatg gggccgtcgg ccgggccgac gagcgcgatg
      181 tagacggtcc cgaccggctt gccgtcctgt tccgtcgggc ctgccacccc cgtcgtcccc
      241 agaccgtacg tcgcgtcgaa agcgcgccgg gcgccgtccg ccatggccac ggcaacgtca
      301 gggtcgaccg ggccgcgctc ggcaagaagc gttgccggga cgtcgaccac cgccgccttg
      361 gagtccgtcg cgtagagcac cgcgccgccg cggtaggtct ccgaggcccc aggcgtcgaa
      421 ctcagtgcgg cgccgagcaa gccgccggtc agtgactctg ccgtcgccac cgtcgcccct
      481 gccgtcagca acagctgatg aacgaccgac tccagcgtgt cgtcgtccac gccgtagatc
      541 acgtcgccca gcgccgcgcg cacccgagtc tcgacgccgg cgatcagctc gcgcgccgcc
      601 gtctccgttg ccgctttggc ggtcatccgt accaacgtct gcccctggga tgcgaggtag
      661 gcaatcgtcg gattcgcggc ggcggcgagt gcggcatcga tctcggcgag ggcctgcgag
      721 acctgcgact cccacacgcc gacggtatgc agccgccgcg acaacacgat cgtgccggga
      781 cccgcacgct cgacaagttc ggggcgcatc caggtgctga agatctcccg catctcctgc
      841 ggcacccccg gcagcgcata cacagtggtt ccacgcgaac cgatcgtcag acgcagcccg
      901 ggagcggtgc cggccggatt cggcaatgct gacgcgccct cggggcgatc agtcatccgc
      961 aggttgttct cggggaaatc gattcgtccc gacgccacgt accgcgcgcg cagcgccgcc
     1021 gcgagctgcg cgtcacgcac gagacggacg ccggccagct cggccagtgc ctcgcgggtc
     1081 aggtcgtcct gtgtcggccc gagaccgccg gtgagcacga caacgtccgc ccgctcgcac
     1141 gcgcgtccga ccgccgcgct gatacggccg atgttgtcac cgactgctgt cgtgagatcg
     1201 acgtcgatcc cgatgtcggc gagctcacga ccgagccacg ccgcattccc gttgacgatg
     1261 tcgcccagca gcagttcggt gccgatcgcg acgatttctg ccttcacagc gcagcgctgg
     1321 cggcacggct cgacgtggcg cgacgcagcc ggatcgcgcg agcaacgtag tcgacgccgg
     1381 tcaccaaggt gataaccacg gcgacgccca tcaggtaggc gcgaagagac gccatcggcc
     1441 cggtcagtgg caagaggtac aggccgatcg cgaccacctg cacgagagtc ttgaccttgc
     1501 cgccacgact ggcggccagc acgccccgcc ggatgaccca gaaccgcagg agcgtgatgc
     1561 cgatctcgcg cgcaaggatg acgaccgtga cccaccacgg caggtcggag atgctggaca
     1621 ggctgatcaa cgcggcgcca gtcagcgcct tgtcggcgat cgggtcggcg atcttgccga
     1681 agtcggtgat cgtgccgcgc cggcgcgcaa cttcaccatc gaagcgatcg gtaagcgacg
     1741 caaggacgaa caccgccgtg gcggcgagcc gccagccatc gtgctcgccg cgtgcgctga
     1801 gcagcagcgc gacaaagatc ggaaccatca ccagccgtgc gacggtcagc gcgttcgcca
     1861 agtttctcac gcccgcctgc gggatcggtg tggtcgcgga ctcggtgatc tcggtcatca
     1921 ggtggccaga ctgtccgcaa gagacgccgt gagcaagagc gcgtcgagtg gtgcggcgac
     1981 cagatccgct cctatcgcgt ccactacctt ggcgcggacg atagtcccgg gcgcgacggc
     2041 aacccccggc aggaacgtcg tgccgtcgac ctcgg
//
LOCUS       DATCBJ010000195         2241 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_234516, whole genome shotgun sequence.
ACCESSION   DATCBJ010000195 DATCBJ010000000
VERSION     DATCBJ010000195.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2241)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2241)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2241
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_234516"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..298)
                     /gene="ssb"
                     /locus_tag="VII50_03980"
     CDS             complement(<1..298)
                     /gene="ssb"
                     /locus_tag="VII50_03980"
                     /inference="COORDINATES: protein motif:HMM:TIGR00621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HEY5050036.1"
                     /translation="MFDTQVTVVGNLVAEPRLAFTNDGQAVASFRLASTPRRFDRATG
                     EWKDGDTLFTSVTCWRALAENVSASLKKGSSVIVLGRLSVRPYETRDGAQRQSVA"
     gene            463..536
                     /locus_tag="VII50_03985"
     tRNA            463..536
                     /locus_tag="VII50_03985"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:497..499,aa:Arg,seq:tct)
     gene            593..1081
                     /locus_tag="VII50_03990"
     CDS             593..1081
                     /locus_tag="VII50_03990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5050037.1"
                     /translation="MELGQYVSDLQRQLVDAAANGAEETRAVAERLAAGLDAATRLVL
                     LDTLSAAVGEITRDLAPGSVDMRLRGREIEFVVTQPNTEPDADERPAPTVDLDDASTS
                     RTTLRLPDALKARVDEAATADGLSVNTWLVRAIAAALQPKQRRSAQRTLRAGDNFAGW
                     AR"
     gene            1158..2009
                     /locus_tag="VII50_03995"
     CDS             1158..2009
                     /locus_tag="VII50_03995"
                     /inference="COORDINATES: protein motif:HMM:NF024743.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HEY5050038.1"
                     /translation="MPTYSTPTPIDLAINLQVGGIDVIASDRADTVVTVSPTNSAKAV
                     DRRGAEETKVDFDGERVTVVGPRPRISWFGPTESVDVLVELPTGSRLTAEIAVGGLRT
                     VGRLGATRIKSSLGAVDVDTTGDLWLRTSHGNATLGAAEGGIEITTDYGRIRVGTVTG
                     DAILKASHGSIVVEQSGGDVDVKLSFGDVEIAKALASVAAKTAYGSIRLGEVSSGSVQ
                     VESGFGPVTIGVRPGVPAWLDLSSKSGHVRNELVGDRAPGAFEQTVAVRARTQLGDIN
                     IQRAQQK"
     gene            complement(2020..>2241)
                     /locus_tag="VII50_04000"
     CDS             complement(2020..>2241)
                     /locus_tag="VII50_04000"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009076675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase"
                     /protein_id="HEY5050039.1"
                     /translation="DDEQKRVALDTIADVNASGLWPGDVVTEVEPVGPFWEAEPEHQD
                     YLVKYPNGYTCHFPRPNWKLPHRSEAVSS"
BASE COUNT          378 a          771 c          748 g          344 t
ORIGIN      
        1 tggcgacgga ctgccgctgg gcgccgtccc tcgtctcata aggccgcacc gacaaccgcc
       61 cgagcacgat gacggacgac cccttcttca gggacgcgga cacgttctcg gcgagcgcgc
      121 gccagcacgt cacgctggtg aaaagggtgt cgccgtcctt ccactccccg gtcgcccggt
      181 cgaaccggcg cggcgtcgaa gcgaggcgaa acgacgcgac cgcctgcccg tcgttcgtga
      241 acgccagccg tggttcggcg acgaggttgc cgaccacggt gacttgcgtg tcgaacatgg
      301 tttctccttc cgggtgcggc tgcctgccgc atgtatagga gagtggcgtc cgcggtcgat
      361 cgagatgggc ggccggtcgc cggttgtgga cagcggcggc ccgcgccgac ctgtggatat
      421 ccagggcgtg gcgtaacctt tggaccgctg tgagcggggt gagcgcccgt agctcagacg
      481 gatagagcac ctgccttcta agcaggacgt cgcaggttcg aatcctgccg ggcgcgcgca
      541 catgacacca ctattgcttg acgagacacc actatggtgt caccatggtg acatggaact
      601 tggacagtac gtcagcgacc tgcagcgcca gttagtggac gccgcagcga acggcgcgga
      661 ggagacgcgc gcggtcgcgg aacgactcgc cgccgggctg gatgccgcga cacggctcgt
      721 gctgctcgac accctgtcgg ccgcggtcgg cgagatcacc cgagacctcg cgcccggatc
      781 ggttgacatg cggctgcgcg ggcgcgagat cgagttcgtc gttacccagc cgaacacgga
      841 gccggacgct gacgagcggc ccgctccgac cgtcgacctc gacgacgcaa gcacctcgcg
      901 cacgacgctt cgcctgccgg atgccctcaa ggcgcgggtg gacgaagcgg cgacggctga
      961 cggcttgtcc gtcaacacat ggctggtccg tgcgatcgcg gccgcgctcc aacccaagca
     1021 acgacgatct gcgcagcgca cgctgcgcgc cggcgacaac ttcgcaggct gggcgcgcta
     1081 actgctcagc ccgcgacatc caccacgcgc acccgcggga taacccgatc atggccagag
     1141 aaaaggaacg cgtcagcatg cccacctaca gcacccctac ccccatcgac ctcgccatca
     1201 acctgcaggt cggcgggatc gatgtcatcg ccagcgaccg cgccgacacg gtcgtgaccg
     1261 tgtcgccgac caactctgcg aaggcggtcg accgccgagg cgcggaggaa accaaggtcg
     1321 acttcgacgg cgagcgggtg accgttgtcg gcccgcggcc ccgcatcagc tggttcggcc
     1381 caaccgagtc ggtcgacgtg ttggttgaac tgcctacggg atcgcgactc accgccgaga
     1441 ttgcggtcgg cggtctgcgc actgtcggac gcctcggcgc cacccgcatc aagagctcac
     1501 tgggtgccgt ggatgtcgac acgaccggag acctgtggct gcgcacctcg cacggcaacg
     1561 cgacccttgg cgccgccgaa ggcgggatcg agatcacgac cgactacggt cggatccggg
     1621 tcggcacggt caccggcgac gcgatcctca aggcatcgca tggctccatc gtggtcgaac
     1681 agtccggcgg cgacgtcgac gtgaagctct ccttcggcga cgtcgaaatc gccaaggcgc
     1741 tcgcctccgt tgccgcgaag accgcgtacg gcagcatccg gctgggagaa gtctcgagcg
     1801 gttccgtcca ggtcgagagc ggcttcggcc cggtcacgat tggcgtgcgg cccggcgtcc
     1861 ccgcctggct cgacctgtcc tcgaagagcg gacatgtgcg caacgagctc gtgggcgacc
     1921 gcgctcccgg cgctttcgag cagaccgtcg ccgttcgtgc gcgcactcag ctgggcgaca
     1981 tcaacatcca gcgcgcccaa cagaagtgac cacctcggcc tatgagctga cggcctcgct
     2041 gcgatgcggg agcttccagt tcggtcgggg gaagtggcag gtgtagccgt tggggtactt
     2101 gaccaggtag tcctgatgtt ccggctccgc ctcccagaac ggcccgaccg gctcgacttc
     2161 ggtgaccacg tcgccgggcc aaagacctga cgcgttcacg tcggcgatgg tgtcgagcgc
     2221 gacgcgcttc tgctcgtcat c
//
LOCUS       DATCBJ010000196         3106 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_123504, whole genome shotgun sequence.
ACCESSION   DATCBJ010000196 DATCBJ010000000
VERSION     DATCBJ010000196.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 3106)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3106)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3106
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_123504"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..710
                     /locus_tag="VII50_04005"
     CDS             <1..710
                     /locus_tag="VII50_04005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5050040.1"
                     /translation="VLAVVAVIVIVAVGVIDFQHTAKHPFDMRTGFPNALATFAGPLV
                     LAAFLFGASLLGADYTSRSLTTLLTWEPRRARLLFSRGATCAAVAFCASFVAMALLVS
                     ALLPAALTHATGHGTAYASVAALATRTALLAAAACAIGVSCAAIGRSTTAAVIGALVY
                     LVVVERAVVGVAPDVGRWLLLNDSLSWVAASPNASNGPGGVGQGHTIATAGLLLLVGV
                     VGIHALATLVLRRRDIV"
     gene            780..1652
                     /locus_tag="VII50_04010"
     CDS             780..1652
                     /locus_tag="VII50_04010"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HEY5050041.1"
                     /translation="MDIGVVYPQTELRGDPSAVRRIGKAVEDMGFDHLLAYDHVLGAV
                     HADRTPPLTGPYTEHHPFHDPFVMFGYLAGITERIGFATGILILPQRQTALVARQAAD
                     VELLSGGRLRLGVGVGWSPVEYEALGQDFHTRGVRQEEQIQLLRRLFTEPVVDFSGSF
                     DRVDRAALVPKPARAIPIWLGGYGEKAFERAARFADGFMFGGGRGVDDAVDKWTVLRG
                     RVCDLGRPADEFGADYLVAPGAGGVADLPAQVDAWRDAGGTHISVVTMGKGFDSIDAH
                     IDYIASVADALSLR"
     gene            complement(1668..>3106)
                     /locus_tag="VII50_04015"
     CDS             complement(1668..>3106)
                     /locus_tag="VII50_04015"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEY5050042.1"
                     /translation="AAVSSVDQPKLLRGRWIQPGGVVVEAGFASALGIHVGDQLTLGA
                     ASFHVVGTAATAAIPSYPDVCAKAEGCFIVGHISSSNPGLIWATEADTSHIAGSSGPV
                     AYFLNLKLNDPVAASTFADRYNGNVSPTAPYLLSWQRIRSGDAEVLAKVQLLLSTGSW
                     LLGLLAIASVAVLVGGRMAEQTRRVGLLKAVGGTPRIVAVVLLLEHLLVGVCAAVVGL
                     VAGWLTAPLIDAPGAGLIGSPNAPSLTAPTVGLVVALAVGIAILATFVPAIRAARQST
                     VAALNDSARPPRRRAWVVGSSAHLPAPLLLGVRLAARRPRRLVLSIFSVAVTTSGLVT
                     VLIIHATASNWSLGPQVMRVTTILSVMLIALAAVNAIFIAWTTALDARHSAALAQALG
                     ATPAQITNGLTIAQLLPALVGALLGIPGGIAIYNNPNHHGATMLPPALWAVATIVLTL
                     LAIAVLTAVPTRIGARRPVAQVLQAEAS"
BASE COUNT          534 a         1032 c         1015 g          525 t
ORIGIN      
        1 tcgtccttgc tgtcgtcgcc gtcatcgtca tcgtcgcggt cggcgtcatc gacttccagc
       61 acaccgcgaa acacccgttc gacatgcgca ctggattccc gaacgcgctc gcgaccttcg
      121 ccgggccgct ggtcctggcg gcgttcctct tcggtgcctc attgctcggc gccgactaca
      181 ccagccgttc gctgaccacc ctgctcacct gggaaccacg tcgcgctcga ctgctcttct
      241 cccgcggggc tacctgtgca gcggtcgcgt tctgcgcgtc atttgtcgcg atggccctct
      301 tggtgtcggc gcttctgccg gctgccctta cccatgcaac aggccacggc accgcctatg
      361 cgtcggtcgc cgctctcgcg acgcgaacgg cgctgcttgc agcggccgcc tgcgcgattg
      421 gcgtgtcttg cgctgccatc ggacgcagca caacggcagc tgtcatcggc gctctcgtct
      481 acctggtggt ggtcgagcgc gccgttgtcg gcgtggcgcc cgacgtaggt agatggctgc
      541 tgctcaacga ttccctgtca tgggtggcgg ccagccccaa tgcgagcaac ggacccggag
      601 gagtggggca gggccacacg atcgccactg ccggactgct tctccttgtc ggcgtcgttg
      661 gcatccatgc gctcgcgacg ctcgtcctcc gtcgccgcga catcgtctag acagcgatca
      721 cttggtcggt ccagagccca agagcatcgc tggcatcaat cgcgtgacag gctggccctg
      781 tggacatcgg tgttgtttat ccccaaaccg aacttcgcgg cgatcccagc gccgttcggc
      841 gaattgggaa agcggtcgaa gacatgggct tcgaccacct gctggcctac gaccacgtgc
      901 tcggtgcagt ccacgccgac cgaacgcccc cgctgaccgg cccctacacg gagcaccacc
      961 cgttccacga tccgttcgtg atgttcggct atctcgctgg catcaccgag cgcatcggtt
     1021 tcgcgaccgg aatcctgatc ctgccgcaac ggcagacggc gctggtggca cgccaagcgg
     1081 ccgatgttga gctgctctcg ggcggccgct tgcgtctggg ggtcggcgtc ggctggagcc
     1141 ctgtcgagta cgaggcgctc ggacaagatt tccacacccg cggggtgcga caagaggagc
     1201 agatccaact cctgcgtcgc ttgttcactg aacctgtcgt cgacttctcc ggcagcttcg
     1261 accgcgtcga ccgtgcggcc ctcgtcccga aacccgcgcg cgctatcccg atctggcttg
     1321 gcggctacgg cgaaaaagca ttcgaacggg ccgcccggtt cgcggatggg ttcatgttcg
     1381 gcggtggtcg cggcgtcgat gacgccgtgg acaaatggac ggtgctacgc ggccgagtat
     1441 gcgacctcgg ccggcccgcc gacgagttcg gggcggacta tttggtcgct cccggcgccg
     1501 gcggtgtcgc agacctccca gcgcaggtcg atgcctggcg cgacgcgggc ggtacccata
     1561 tctcggtggt cacgatgggc aagggtttcg actcgatcga cgcgcacatc gactacatcg
     1621 catcggtcgc agacgcactc agcctgcgct gacgtccgcg tcggaggtta agaagcttct
     1681 gcctgcagaa cctgagcgac cggtcgacgt gcaccgatac gagtcgggac ggcggtgagc
     1741 acagcgatcg caagcagcgt gagcacgatc gtcgcgacgg cccaaagcgc aggcggcagc
     1801 atcgtcgcgc cgtgatggtt tgggttgttg tagatggcga tcccgccggg gatgccgagc
     1861 agcgctccga cgagagcagg aagcagttgc gcgatcgtca aaccgttggt gatttgcgcc
     1921 ggggtggctc ctaacgcttg cgcgagcgcg gccgagtgtc gtgcgtccag cgcggtcgtc
     1981 cacgcgatga agatggcgtt gaccgcggcg agcgcgatga gcatcaccga caggatggtg
     2041 gtcacccgca tgacctgagg tccgagcgac caattcgatg cggtcgcatg aatgatgagc
     2101 acggtgacga gcccgctcgt cgtcacggcg acgctgaaga tgctcaacac aagtcgtcgg
     2161 ggtcggcgcg cagcgagccg gacgccgaga agcagcggcg ctggcaaatg cgccgacgat
     2221 ccgactaccc acgctcggcg ccgtggtggg cgcgccgaat cgttgagggc cgcgacagtg
     2281 ctttgacgcg cggcacggat cgccggcacg aacgtcgcca ggatcgcgat gccgaccgca
     2341 agtgcgacaa ccaagcccac cgtgggtgcg gtaagcgatg gcgcgttcgg cgaaccgatg
     2401 aggccagcgc caggggcgtc gatcaacggc gccgtcagcc atccggcgac cagccctaca
     2461 accgcagcgc atacgccgac aagaaggtgc tcgagcagca gcacgacagc aacgatccgc
     2521 ggcgtgccac cgacagcctt caagagaccg actcggcgtg tttgctcagc catgcgaccg
     2581 cccaccagca cagccacact cgcgatcgcc agaagcccga gcagccagct accggtggag
     2641 aggagcagct gcactttggc cagcacctcc gcgtcgccac tgcgaatgcg ctgccaagaa
     2701 agcaggtacg gagcagttgg tgaaacgttg ccgttgtatc tgtcggcgaa cgtgctcgcg
     2761 gcgaccggat cgttgagctt caggttcaag aagtacgcga ccggcccgga gcttccagca
     2821 atatgcgacg tatcggcttc ggtcgcccag atgagtccgg ggttggatga actgatgtgc
     2881 ccgacgatga agcagccctc ggctttagcg cacacatcgg gataggaggg gatcgcggca
     2941 gtggccgccg tccccacgac gtggaaactg gccgcgccga gggtgagctg atcgccgacg
     3001 tgaataccca atgcactcgc gaagcccgct tcgaccacca cgccacccgg ttgaatccag
     3061 cggccgcgca gcagcttcgg ctgatccaca gacgagaccg cagcgg
//
LOCUS       DATCBJ010000197         2918 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_270713, whole genome shotgun sequence.
ACCESSION   DATCBJ010000197 DATCBJ010000000
VERSION     DATCBJ010000197.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2918)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2918)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2918
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_270713"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            327..1910
                     /gene="istA"
                     /locus_tag="VII50_04020"
     CDS             327..1910
                     /gene="istA"
                     /locus_tag="VII50_04020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011544394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HEY5050043.1"
                     /translation="MERHPMRKVREILRLRFERGLSQRQISASTGVSKGAIGEYMRRA
                     AAAGVTWEVARALDDSEVEARLFRYPGRNEPPRRVPIDLPWVHRELRRTGMTLQQLWI
                     EYRGGAAAGLPSGGTPYGYSQFCDLYADFRQRADLSMRQVHRAGEKIFIDYSGKRPRI
                     WSAETGEAIEVELFVAVLGASNYTFAEATRTQRLEDFCASTVRALEFFGGVPKVAVPD
                     QLRSAVSGPDRYDPEINPTYAELAQHYDLAIVPARPGKPRDKAKVEGAVLVAQRWILA
                     CLRNRTFFTLDDLNIAIAELVEKLNERPFKKLEGCRRSAFETLDRPALRPLPTSRWEL
                     ARWAKAKVNIDYHVEYDGRIYSVPHALVGERVEVRATNAVVEIFHRARRVASHARLWG
                     PRGSVSTIAEHRPRSHREYGAWPPSRIIGWAASIGPSSAALVEQILLGRREPESAYRS
                     CMALIRSAKAYDSARFDAACQRAIAIGSPNRKSVLAILSSGLDAIAPEPVDAQLPLSV
                     VHENVRGGDYYDRKETDPS"
     gene            1907..2668
                     /gene="istB"
                     /locus_tag="VII50_04025"
     CDS             1907..2668
                     /gene="istB"
                     /locus_tag="VII50_04025"
                     /inference="COORDINATES: protein motif:HMM:NF013828.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element helper ATPase IstB"
                     /protein_id="HEY5050044.1"
                     /translation="MTDEETIQKLLDLRLTTMAQAFRDLLAEPPGNQRSFTEKIGTMV
                     DREWTERDNRRLGRLLHAARLTIREASLENVWCEPHRGLDKAVMRDLATCQWIRKKHN
                     VIVVGKTGVGKSYLGAALAQAACRKGLRALCTRVPRLVHELAIARADGTYTTMLARLA
                     KLDVLVLDDFLIAPLKDTERRDLLEVLEDRYGVSSTVVTSQVPTKNWHEMLADPTIAD
                     AICDRLVHNAHVIALRGPSGREKKGLQIDSSQPSA"
     gene            complement(2653..>2918)
                     /locus_tag="VII50_04030"
     CDS             complement(2653..>2918)
                     /locus_tag="VII50_04030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5050045.1"
                     /translation="TVKPNRRSLEIARLSRFRFASNLASQKSRLRLGTVARTQPLRIP
                     LDPGTRSGVIRALVPVDVGAVGAKRRALSGVGAKRRVKAHAEG"
BASE COUNT          511 a         1002 c          962 g          443 t
ORIGIN      
        1 tttctcaacc cgtggatggg gacgggaacg ggaccgcgga ctcgaccttc acccgccgcg
       61 ccttccccaa catcaagcac accattgtca gctgccagag accaaccgca accgctccgt
      121 gatcgtgcgt attccggtcg catccgggca ccgattccgg cgggacctgg gcagcgattc
      181 cgaaatgacg cgggcgctaa atccggagcg atccgggcac tgattccggt gtgacgcggg
      241 cgctaactcc gggccatctg ggcaccggag cggggcgact ggtgggggct ctttggcgcg
      301 gcggccggtg acggcatcgt cgcgcgatgg agcgtcaccc aatgcgaaaa gttcgagaga
      361 ttcttcggct acggttcgag cgtggcctga gccagcgcca gatttcggcg tcgactggcg
      421 tatcgaaggg cgcgatcggc gagtacatga ggcgcgcggc ggcggccggc gtcacgtggg
      481 aggtcgcgcg ggccctcgac gacagcgagg tcgaggcccg cctcttccgg tacccgggcc
      541 gcaacgagcc gcctcggcgc gttccgatcg atctgccctg ggtgcatcgc gagctgcgtc
      601 gcaccggcat gaccttgcag cagctgtgga ttgagtaccg gggcggcgcg gccgctggcc
      661 tgccgtcggg cggaacgccc tacggctaca gccagttttg cgatctgtac gcggacttcc
      721 ggcagcgtgc ggacctttcg atgcgccagg tgcaccgcgc cggcgagaag atcttcatcg
      781 actactcggg caagaggcca cggatctggt cggcggagac gggcgaggcg atcgaggtcg
      841 agctcttcgt cgcggtgctg ggcgcctcga actacacatt cgcggaggcg acgcggacgc
      901 aaaggctgga ggatttctgc gcgtcgacgg tgcgcgcgct ggaattcttc ggcggtgtgc
      961 ccaaggtggc ggtgcccgat cagctgcgca gcgccgtcag cggccctgac cgctacgacc
     1021 cggagatcaa tccgacatac gccgagctgg cgcagcatta cgacctggcg atcgtgcccg
     1081 cgcgtcctgg caagccgcgc gacaaggcga aggtcgaggg cgcggtgctg gtggcacaac
     1141 gctggatcct ggcgtgcctc cgcaaccgca cctttttcac tctcgacgac ctgaacatcg
     1201 cgatcgccga gctggtcgag aagctgaacg aacgcccctt caagaagctg gagggctgcc
     1261 gccgctccgc cttcgagaca ctggatcgcc cagcgctgcg cccgctgccg acgtcgcggt
     1321 gggagctggc gcgctgggcg aaggccaagg tgaacatcga ctatcacgtc gagtacgacg
     1381 gccggatcta cagcgtgccg cacgcgctcg tcggagagcg cgtcgaggtg cgtgcgacga
     1441 acgccgtcgt cgagatcttc catcgcgccc gtcgcgtcgc cagccatgcg cggctgtggg
     1501 ggccgcgcgg atcggtcagc accatcgccg agcaccggcc gcgctcgcac cgcgagtacg
     1561 gcgcctggcc accgtcgagg atcatcgggt gggccgcctc gatcggcccc tcatcggccg
     1621 cgctcgtcga gcagatcctg ctgggccggc gcgagccgga gagcgcgtac cgatcgtgca
     1681 tggcactgat ccgaagcgcc aaggcgtacg acagcgcgcg cttcgacgcc gcctgccagc
     1741 gcgcgatcgc gatcggctcg ccgaaccgca agagcgtcct tgcgatcctg agcagcgggc
     1801 tcgatgccat cgcgcccgag cccgtcgacg cccaacttcc actcagcgtc gtgcacgaga
     1861 acgtgcgcgg cggcgactac tacgaccgaa aggagacaga cccatcatga ccgacgaaga
     1921 aaccatccag aagctgctcg atcttaggct caccaccatg gcccaggcct tccgcgacct
     1981 gctggccgag ccgcccggca accaacgatc tttcaccgag aagatcggaa ccatggtcga
     2041 tcgcgaatgg accgaacgcg acaaccgccg actcgggcgc ctgctgcacg ccgcgcgcct
     2101 cactatcagg gaggcgtccc tcgagaacgt ctggtgcgaa ccgcaccgtg gtctcgacaa
     2161 agcggtcatg cgcgatctcg cgacgtgcca gtggatccgg aagaagcaca acgtgatcgt
     2221 cgtcggcaag accggcgtcg ggaagtcgta tctcggcgcg gcgctggcgc aggcggcttg
     2281 ccgcaaaggt ctccgcgccc tgtgcacccg cgtgccgcgg ctcgtgcacg agctcgcgat
     2341 cgcgcgagcc gacggcacct acaccacgat gctcgcccgc ctcgccaagc tcgatgtcct
     2401 cgtgctcgac gatttcctga tcgcgcctct caaggacacc gagcgtcgcg acctgctcga
     2461 ggtgctggag gatcggtacg gcgtctcgtc gacggtcgtg acctcacagg tcccgacgaa
     2521 aaactggcac gagatgctgg ccgaccctac catcgccgac gccatctgcg accgcctggt
     2581 ccacaacgcc cacgtcatcg ccctcagagg cccctcgggc cgcgaaaaga aaggactcca
     2641 gatcgactcg tctcaaccct cagcgtgagc cttcacccgt cgcttcgctc cgacaccaga
     2701 cagagcccgt cgcttcgctc cgacggcgcc cacgtcaact ggaacgagcg cccggatcac
     2761 gccggaacga gtgcccggat cgagcggaat acgcagcggc tgcgtccgtg cgacggtgcc
     2821 caaacgcaat cgagacttct ggctggccaa attcgaggcg aaccgaaatc gcgacaagcg
     2881 agcgatttcc aggctgcggc ggttcggctt tactgtcg
//
LOCUS       DATCBJ010000198         2020 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_486241, whole genome shotgun sequence.
ACCESSION   DATCBJ010000198 DATCBJ010000000
VERSION     DATCBJ010000198.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2020)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2020)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2020
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_486241"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..732
                     /locus_tag="VII50_04035"
     CDS             <1..732
                     /locus_tag="VII50_04035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011718909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEY5050046.1"
                     /translation="IVLATKFGMPAGGMTLPAHEARGSRRYIRASIEGSLRRLRTDHI
                     DLYQMHRPDSLTPIEETLAALDEVVREGKVRYIGSSQFGGWQVIDADWTARANGGARF
                     VSTQNQYSLLERVAETELLPAAARAGVGFIPFYPLARGLLTGKYQRGEAPPDGTRLAG
                     DAQLLESANWDVIEALQKFAAARGISMLEVAIGGLAAQPGVSSIIVGATKPEQVLANA
                     KAAAWEPAAVDLVELDDITGAARRS"
     gene            751..1026
                     /locus_tag="VII50_04040"
     CDS             751..1026
                     /locus_tag="VII50_04040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019049511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2516 family protein"
                     /protein_id="HEY5050047.1"
                     /translation="MLHISASLISVVLLAALVLQLYALVDAALVPAAAYVAAGKLTKP
                     AWLAILAVAIVVVLLSGWFGPLGLAAIIATIVYFVDVRPALRRVRGR"
     gene            1087..>2020
                     /locus_tag="VII50_04045"
     CDS             1087..>2020
                     /locus_tag="VII50_04045"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 87 protein"
                     /protein_id="HEY5050048.1"
                     /translation="MSSVLSEAAVSSADWLRREEEQLFGRYAVAAQRFPRSFRFRVPL
                     VLAVYLLVALVGQALNGPGGDATVFGGAGNTLFSTHWADTFASPVLQSGPIFVLLEGI
                     GTRLSSAVGLPYWSGGGMVTALAVAVLMVVACRAVFAVYGLAGRTAAAREFGMCMVFA
                     LTGGLWIAAMYAHPDDLMVGLLLLIAASRAAAGRHGRAGAFFGLAAGVKLWALIAIAV
                     LLVRASRRASVQAGLMAAATVALCYGPFIVFGRFETFRGSWQIEHDAPLNLIFHAGTD
                     FSWELRLFQAALATTVGLVVLLRTRGRNCAVWLVP"
BASE COUNT          270 a          645 c          695 g          410 t
ORIGIN      
        1 atcgtccttg ccaccaagtt cggcatgccg gcgggtggta tgaccttgcc ggcgcacgag
       61 gctcgcggct cgcgacgcta catccgggcg tccatcgaag gatcgctgcg acgcctgcgt
      121 accgaccaca tcgacctgta ccagatgcac cggccagaca gcttgacgcc aatagaggaa
      181 acccttgctg cgctggacga agtcgtgcgc gaaggcaagg ttcgttacat cggttcatcg
      241 caattcggtg gctggcaggt catcgacgcg gactggacgg cgcgtgcgaa cggcggcgcg
      301 cgtttcgtga gtacacagaa ccagtacagc ctcttggaac gtgtcgccga gaccgaactg
      361 ctgcccgccg cggcgcgtgc cggcgtcggc ttcattccgt tctatccgct ggcgcgtggc
      421 ttgctcacgg gcaagtatca gcgtggcgaa gcgccccccg acggcacccg gctcgccggc
      481 gacgcacagc tactcgagag cgccaattgg gatgtgatcg aggcgttgca gaagtttgcc
      541 gcggcacgcg gcatcagcat gctcgaggtc gcgatcggcg gcctcgcggc gcagcccggt
      601 gtcagcagca tcatcgtcgg cgcgaccaag cccgagcagg tgttggcgaa cgcgaaggcg
      661 gccgcctggg agccggcggc cgtcgatctg gtcgagctcg acgacatcac gggcgccgca
      721 cgccgctcct gaggcagtac cttgggtggt gtgctgcaca tctcagcgtc gttgatcagc
      781 gttgtgctgc ttgcggcgtt ggtcctccag ctctacgcgc tcgtcgatgc cgcgctcgtt
      841 ccggcggccg cgtacgtagc ggccgggaaa ctgacgaagc cggcctggct agcgatcctt
      901 gctgtcgcca tagtcgtcgt cttgctgagc gggtggtttg gcccgttggg gctggccgcg
      961 atcatcgcca ccatcgtcta cttcgtcgac gtgcgtcctg cgttgcggcg ggttcgcgga
     1021 cggtagccgc tcccctgggc gcgtgctcgg ctttagaggt gccgggaatc cgtcgacgca
     1081 ttctgcatgt cgtctgtctt gagcgaggcc gcggtctcgt ctgcggactg gctacgccgc
     1141 gaagaggagc agctgttcgg ccgctacgca gttgccgcgc agcggttccc gcggtcgttc
     1201 cgcttccgcg ttccgctggt actagcggtg tatctcttgg tcgcgttggt gggacaggct
     1261 ctgaacggtc cgggtggcga cgcaaccgtc ttcggcggcg cgggcaacac cttgttctca
     1321 acgcattggg ccgacacgtt cgctagcccc gtcttgcaga gcgggccgat attcgtcctc
     1381 ctcgagggca tcggcactcg cctcagttcc gccgtcggct tgccctattg gtccggcggc
     1441 ggcatggtta cagctttggc agttgcggtg ctcatggtcg ttgcgtgccg cgctgttttc
     1501 gccgtctacg ggctcgctgg ccggacggca gctgcgcgcg agttcggtat gtgcatggtg
     1561 tttgcgctga ccggcggact ctggatcgcg gccatgtacg cgcatcccga tgacctgatg
     1621 gtcggcctgt tgctgctgat tgcggcgtcc cgcgcggctg ctggccgcca cggccgagct
     1681 ggcgcgttct tcggactcgc cgcgggggtc aaactgtggg cgctgatcgc gatcgccgtg
     1741 ctgcttgttc gcgcgtcgcg ccgtgcctcc gtccaagcag gcttgatggc cgcggcgaca
     1801 gtcgcgctgt gctacggacc gttcatcgtg ttcggtcgat tcgagacctt ccggggaagc
     1861 tggcagatcg agcacgacgc gccgctgaac ctgatctttc acgctggcac cgatttcagc
     1921 tgggagctgc gtctgttcca ggccgccctc gcgacgaccg tcgggttggt ggtcttgctc
     1981 cgaacacgag gacgcaactg tgcggtgtgg ctggtgccag
//
LOCUS       DATCBJ010000199         4855 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_519355, whole genome shotgun sequence.
ACCESSION   DATCBJ010000199 DATCBJ010000000
VERSION     DATCBJ010000199.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 4855)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4855)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4855
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_519355"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..712)
                     /locus_tag="VII50_04050"
     CDS             complement(<1..712)
                     /locus_tag="VII50_04050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5050049.1"
                     /translation="MLPDGADSRGDPLEQDAPDNPVDDPAILSWFLAEGDRANPWTDL
                     RAFTTGNLVVPLIDGRTYFARLCVELEATAAGDQVYFLDFRGDFTERLAGPGSEVGEV
                     LGRAAHRGVHVFGLLWRSQPKILKQSEAANAEFVRRIDEDGGQVLLDARTRRAGSHHQ
                     KLVVIRHPGSPNRDVAFLGGIDLGYSRGDDSQHDGDPQPMEFAAAYGPRPPWHDIQAE
                     VHGPAVHDLEHTFRERWYG"
     gene            complement(1084..1896)
                     /locus_tag="VII50_04055"
     CDS             complement(1084..1896)
                     /locus_tag="VII50_04055"
                     /inference="COORDINATES: protein motif:HMM:NF018436.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1206 domain-containing protein"
                     /protein_id="HEY5050050.1"
                     /translation="MVATRTATQVERAATGSAAASIARAGLVARGVIYLLIGWVALML
                     ALGRSNAEADQRGALKTLAGKPYGSTALWLLVVGFAAYALWRISEAFFGVTGEGSGAG
                     PRAKSAFRGVVYAFFAVTTVSVLHGSGGSQATTQQDATARVMHHSGGRWAVGLVGLVV
                     VIVGLTLILQGLRRSFVKTLRTGEMSNRTRAIVVKLGVIGTTARGIVFALAGGLVIEA
                     AVKFDPAKARGLDEALRTLRDRPYGAFALALAALGLIVFGLYGLCEARWRKV"
     gene            2036..2791
                     /locus_tag="VII50_04060"
     CDS             2036..2791
                     /locus_tag="VII50_04060"
                     /inference="COORDINATES: protein motif:HMM:NF013718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEY5050051.1"
                     /translation="MLDRSTETRRRRLDPDTRLGLELTLWGIAAFVVLVPFGVLLALV
                     RSHSSGLQSVDRTVGAHAHTLAVGHPGLVRFLNDVSTAGAPRTFWVLVALIVVVLVIR
                     RAGRLALWAAVTMAGAALLDNVIKTVADRARPYFADPVATAPGKSFPSGHALESLAGC
                     AILLAVALPVMRPLWRRITVAGAVAMVGLIGFARVTLGVHYLSDVVGGWIFAAGWVAA
                     TMAAFRIWRKKADPQSNQPGALDAAGSQRLVTS"
     gene            2788..3495
                     /locus_tag="VII50_04065"
     CDS             2788..3495
                     /locus_tag="VII50_04065"
                     /inference="COORDINATES: protein motif:HMM:NF013718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEY5050052.1"
                     /translation="MKRTALLLIVGALVLLAAMAGFGLLDVHVLTHSTFGHVDRSVDQ
                     TLSHHRTRWLNAVTLGATDSASTYPVAGAALALIVAARLLFKRWREAVFVLCALVGEV
                     TIFVTTTLLVHRARPNVVRLDHAPPTSSFPSGHTAAAVALCGAAAILVVRYSTRAAWR
                     RVAVALAIVLPLIVAGSRLYRGMHYPTDVLAGALLGVSWLRICTRAVLDSAVRRISAV
                     HPPGTRPRTMPAARAHA"
BASE COUNT          798 a         1623 c         1571 g          863 t
ORIGIN      
        1 tgccgtacca acgctcacga aaagtgtgct ccaggtcgtg aaccgcagga ccgtgaacct
       61 cagcctggat gtcgtgccag ggcggccgcg gcccataggc cgccgcgaac tccatcggct
      121 gcggatcgcc atcatgctgc gagtcgtcac ctcggctgta cccgagatcg atgcccccga
      181 ggaacgcgac atctcgattc ggggagcccg ggtgtcgaat gacgacgagc ttttgatgat
      241 ggcttcccgc gcggcgcgtc cgcgcatcca gcaacacttg accgccatcc tcgtcaatgc
      301 gtcggacgaa ctccgcgttc gccgcctcgg actgcttcaa gatcttgggt tgcgaacgcc
      361 acagcagacc gaagacgtgg accccccggt gcgcggcccg gcctagcacc tcaccgacct
      421 ccgaccccgg gccggccaga cgctccgtaa agtcgccgcg aaagtccagg aaatagacct
      481 ggtccccggc ggcggtcgct tcgagctcga cgcacagccg agcgaaatag gttcgaccgt
      541 cgatcagcgg cacaaccaag ttgccggtcg tgaaagcacg caggtcagtc cacgggttgg
      601 cacggtcacc ctcagctaga aaccacgaga ggatcgctgg gtcatctacg gggttgtcag
      661 gggcgtcctg ttcaagcggg tccccgcgag agtcagcgcc atcgggaagc atccccggat
      721 tgtgtaccct ccagatcctt cacgtccgat gcttgagcca accaccccag atgtgctgtc
      781 aacgaagtcg gtgacatcga acaaggagcg cattgagagc tcgcatggat tcggcgcacc
      841 gtcgtcgtcc gcgacagttc gacgagcgtc gagtcgacta gccgccccgt ctctgggatc
      901 ggttaggacc ggggcccatc gttgcagcgg tggtctggcg tcgggctcgt agacggcgcg
      961 gcagaacaac cgtgcccagg cggctcgaaa gcctcgcggg gcagcgattt tttggggttg
     1021 cagcgttgag tggcggtcaa gaaccaatcg tcgtgctgca cggctgcgcc acctccgccc
     1081 tcgtcacacc ttgcgccagc gcgcctcgca caacccgtac agcccgaaga caatgagccc
     1141 aagcgccgcc agcgcgaggg caaatgctcc gtagggccga tcgcggagcg ttcgcaaggc
     1201 ttcgtcgagg ccccgcgcct tcgctggatc gaacttaacg gccgcttcga tcaccagccc
     1261 gcccgcgagc gcaaagacga tgccccgcgc ggtggtaccg atcaccccga gcttcaccac
     1321 aatcgcacga gttcggttgc tcatctcccc ggtgcgcaac gtcttgacga aagagcggcg
     1381 aagaccctgg agtatcagcg tcaatccaac gatcacgacc actaggccga cgagacccac
     1441 tgcccagcga ccaccgctgt gatgcatcac ccgtgcagtc gcgtcttgct gcgtcgtcgc
     1501 ctggctccct cccgaaccgt gcagcaccga caccgtcgtc acagcgaaga acgcgtagac
     1561 gacgccacgg aacgcggact tcgcgcgagg cccggcaccc gaaccctcac ccgtgacgcc
     1621 gaagaaggcc tcgctgatgc gccacagtgc gtatgccgcg aagcccacaa ccagcagcca
     1681 cagcgccgtc gagccgtagg gcttgcccgc caaggttttc aacgcaccgc gctggtcggc
     1741 ctcggcgttg ctgcgcccga gcgcgagcat cagcgcgacc cagccgatca acagatagat
     1801 cacgccacgc gccacaagcc cagcacgcgc gatggatgcg gccgctgatc ctgtcgccgc
     1861 gcgctctact tgtgtcgccg tcctcgtcgc aaccacgtgt cccgctctcg atcgtgaggc
     1921 cgggcttcat cccgccactg aactcggctt acccgatccg atagcgcgca atcaacgcga
     1981 gtagtggaag tagcaaccac gggtaaagac gcaggcaacg gggtaaacac gccccatgct
     2041 cgacagaagc acggagactc gtcgacggcg actcgatccg gatacccgct tagggctcga
     2101 attgaccttg tggggcatcg cagccttcgt cgtgctggtg ccgttcggtg tcctgctcgc
     2161 gttggtgcgc tcccatagtt ccgggctgca aagcgtcgac cggactgtcg gcgcacacgc
     2221 gcacacgcta gccgtcggcc accccgggct cgtgaggttc ctcaatgatg tgagtacggc
     2281 cggtgcgccg cgaaccttct gggtgctggt ggctctgatc gttgtggtgt tggtgatcag
     2341 gcgggccggc aggctcgctc tatgggccgc agtcaccatg gcgggcgcgg cgttgctcga
     2401 caacgtcata aagaccgtgg ctgatcgcgc gcgaccctat ttcgccgatc cggtcgcgac
     2461 cgcgcccggc aagagctttc cgtccgggca cgcgctggaa tcccttgccg ggtgcgcgat
     2521 tctgctcgcg gtcgcactac cggtcatgcg cccactctgg cgccgaatta ccgtcgcggg
     2581 tgcggtcgcg atggtcgggt tgatcggctt cgcccgggtg accttgggtg tgcactacct
     2641 cagcgacgtc gtcggtggct ggattttcgc cgccggctgg gtcgccgcga cgatggcggc
     2701 atttcggatc tggcggaaga aggccgaccc gcagtcgaac cagcccggcg cacttgacgc
     2761 cgcgggtagc caacggttgg tgacgtcatg aagcggaccg cgctgctcct catagtcggc
     2821 gccctcgtgc tgctcgccgc gatggccggt ttcggcctgc tcgacgtcca cgtgctgacg
     2881 cacagtactt tcgggcacgt cgaccgctcc gtcgaccaga cgctgtccca tcaccggaca
     2941 cgttggctga acgcggtcac gctcggggcg acggactcgg cctcaacata tcccgttgcc
     3001 ggcgcggcgc tagccctcat cgtggcggct cggctgctct tcaagcgctg gcgcgaagcc
     3061 gtgttcgtgc tatgcgcact cgtgggcgag gtcacgatct tcgtgacgac gacgttgctg
     3121 gtgcaccggg ctcggccaaa cgttgtcagg ttagaccacg ccccgccgac gtcgagtttc
     3181 ccgtcggggc acaccgcggc ggcggtcgcg ctttgcggcg cagccgccat cctcgtcgtc
     3241 cgctacagca cgcgagccgc ctggcgccgg gtcgccgtcg ccttggcgat cgtgttaccg
     3301 ctcatcgtcg ccggctcgcg gctctatcgc ggcatgcact acccgaccga cgtactcgcc
     3361 ggcgcgttgc ttggcgtcag ctggcttcga atatgcacgc gcgcggtgct cgattcggcg
     3421 gtgcgccgga tcagcgccgt gcatccgcct ggcacgcgac cgcgcaccat gcccgctgct
     3481 cgcgcccacg catgaagact gcggcgtttc tcgtcaactc caaaagtgca cccacgtcga
     3541 gccatcagga acaccgcggg atcatgaccc gctggccggg accgctgtgc ttggagacca
     3601 cagtcgagta caccggcccg agaccggtca ggcaccgcag ccggtgttga cccatggtcg
     3661 ctttcgcgcc gacaccaccg ttgcggcgcc gccgggctgg tcctgccgcg gcggtgtggt
     3721 acgtcgtccc cctaggtaca caagatccaa tcttcacggc ctagggacga gcgcgggtgg
     3781 gcgaaatcct tgctgtgcaa gggttttcgg tggagcgggt gaccgggatc gaaccggcat
     3841 ggtcagcttg gaaggctagc gggacacgtt ctagtttgag cgtgaaaatg gccgttgacc
     3901 tgcgcatatc cgttttgatc ttgaccgctg tcgtccgtag tagtcccgat taccgcccgt
     3961 acggccccgc taaggacgcg cttcgcgcgt ttgccggtcc gcgcccttgg gatgtgctga
     4021 ggtgatcgca atggccccgt gaactggtcg ggctctacgc tggaggtgtg attttcgatc
     4081 gcatcaccgt cgagccggac aagatggccg gccagccttg tatccgcggg ctgcggatcc
     4141 cggtggccac ggtggtggcg ctggtggccg aacgggatga ccacatccga gatcctcgct
     4201 ggtctgccga agttggaagc cgaggatgtg gcgcaggccc gtcggtacgc ccgcggccgc
     4261 ggtccgggag cgggagctgc cgttgcgtca tcccccgtga ggttcttggt cgacgcgaac
     4321 ccgtcgccac gatcggcggt cagcgtgacc caggcaggac acgacgccgt cctacccgtt
     4381 gggcgccgtg gttcgctaat cgaatcgagt gaggcggcag gatcacgacg gcgacgaagg
     4441 cccagcgatc gtgggcgcgt tcgcggccac tgtgtaatgg gtcggcttcg gcatttcgat
     4501 gacgctggtg gcagcggccg agctgactcg gcggtggcgt gggttgcagc gatcgagcgc
     4561 atggtcgggc ttccgccgct gacggcacgt gtcggcgtac cgtcgtccgg ttgcagggat
     4621 aggtccaccc gctaatcggt gtcaccaagc cgggtacagg ctcgccccac ttgccgccga
     4681 atgggggaat tatcggttta cagagcgctc tgtgatggta tggtcactgt tggtagtccg
     4741 tctagaccgt ctgcggcccc cttcctggcc gttggcggtg gcatggacga cgacaagttg
     4801 tctgcggtgc tgagcgagtt cgcgcggacg ttggccactg atttcccgat tcagg
//
LOCUS       DATCBJ010000200         2337 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_154216, whole genome shotgun sequence.
ACCESSION   DATCBJ010000200 DATCBJ010000000
VERSION     DATCBJ010000200.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2337)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2337)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2337
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_154216"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2337)
                     /locus_tag="VII50_04070"
     CDS             complement(<1..>2337)
                     /locus_tag="VII50_04070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020657732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase central domain-containing
                     protein"
                     /protein_id="HEY5050053.1"
                     /translation="LPHAVLMMIPEAWEKNTEMDAARRAFYEFHASVMEPWDGPACVT
                     FTDGTLIGAVLDRNGLRPARYWVTDTGLVVLASEVGVLDIDPASIVRKGRLQPGRMFL
                     VDTAAGRIVDDAEIKSQLAAEHPYDDWLHAGLLKLPKLPDREHISYSHESVLRRQQTF
                     GYTEEELRILLAPMASTGAEPIGSMGNDSPLAALSVRPRLLFDYFSQLFAQVTNPPLD
                     SIREELVTSLAGAIGPEQNLLDPGPISCRQIVVPFPVIENDELAKILHVNDDGDLPGL
                     APFVVRGLYDVDGGGEALEQRLSEICAETSAAIDAGARIIVLSDRDSDRERAPIPSLL
                     LTAAVHHHLIREKTRTKVGLIVEAGDVREVHHVALLIGYGAAAVNPYLAMETVEDMTR
                     TGAITGIDGGTAVHNLVKALGKGVLKVMSKMGISTVASYTGAQVFEALGLSHTVVDRY
                     FTGTSSKLGGVGLDVIAREVESRHTHAYPMGGVQQSHRRLQIGGDYQWRREGEPHLFN
                     PETVFRLQHSTRTGRYELFKDYSKLVDDQSRELMTLRGLFKLRTGLRPPVPLDEVEPI
                     SDIVKRFSTGAMSYGSISSEAHENLAIAMNSLGARSNTGEGGEEADRLYDNRRSAVKQ
                     VASGRFGVTSEYLTGADDLQIKMAQGAKPGEGGQLPGHKVYPWIAKTRYSTPGVGLIS
                     PPPHHDIYSIEDLKQLIHDLKNANPRARVHVKLVAEVGVGTVAAGVAKAHADVVLISG
                     HDGGTGAAPLTSLKHAGGPWELGLAETQQTLIAHGLRER"
BASE COUNT          420 a          761 c          727 g          429 t
ORIGIN      
        1 ccgctcgcgc aagccgtgcg cgatcagcgt ctgctgcgtt tcagcaagac cgagttccca
       61 tgggccgcct gcgtgcttca gcgaggtaag cggagccgca ccggtaccac cgtcgtgacc
      121 agagatcagg acgacgtcgg cgtgcgcctt cgcgactccg gcagcgactg tgcccacacc
      181 gacctcggca acgagcttga cgtgcacccg cgccctcggg ttggcgttct tcaggtcgtg
      241 gatcagttgt ttcaagtctt cgatcgaata gatgtcgtga tgcggcggcg gcgagatgag
      301 tccgacccct ggcgtggagt accgcgtctt cgcgatccat gggtacacct tgtgaccagg
      361 aagctggccg ccctcgccgg gctttgcgcc ttgcgccatc ttgatctgaa ggtcgtcggc
      421 acccgtgagg tactcactcg tcacaccgaa ccggccgctc gctacttgtt tgaccgcaga
      481 gcgtcggttg tcgtaaagcc gatccgcctc ctcgccgccc tcgcccgtgt tcgaccgcgc
      541 gccgagtgag ttcatcgcga tggcgagatt ctcgtgagcc tcgctgctta tcgagccata
      601 actcattgcg cccgtggaga aacgcttgac gatgtcagaa atgggctcga cttcgtcgag
      661 cggcaccggt ggccgcagac cggtgcgcag cttgaacaga ccacgcaacg tcatgagctc
      721 gcgcgactgg tcgtcgacaa gtttgctgta gtccttgaag agctcgtagc ggcccgttcg
      781 cgtcgaatgc tgcagccgga acaccgtctc ggggttgaac aggtgcggct cgccctcacg
      841 ccgccattga tagtcgccgc cgatttgcaa tcgccggtgc gactgttgca ccccgcccat
      901 cgggtaggcg tgcgtgtgcc tcgactcaac ctcgcgggcg atcacgtcaa gcccgacgcc
      961 gccgagcttc gacgacgttc cggtgaagta gcggtcgacc acggtgtggg acagcccgag
     1021 ggcttcgaac acctgtgcgc cggtgtagga cgcgaccgtc gagataccca tcttcgacat
     1081 caccttcagc acgcccttgc caagcgcctt caccaagttg tgcaccgcgg tgccaccgtc
     1141 gatgccggtg atcgctccgg tgcgcgtcat gtcttcgacg gtctccatcg ccagataggg
     1201 gttgaccgca gcggcgccgt atccgatcag cagcgcgaca tgatgaacct cgcgaacgtc
     1261 gccggcctcg acgatgaggc caaccttcgt gcgcgtcttc tcacgaatga ggtgatggtg
     1321 caccgccgcg gtcagcaaca acgacgggat cggtgcgcgc tcacgatctg aatcgcgatc
     1381 ggacaacacg atgatgcggg caccggcgtc gatcgccgcc gaggtctcgg cgcagatctc
     1441 gctgagccgc tgctcgagcg cttctccccc accatcgacg tcgtagaggc cacgcaccac
     1501 gaacggggcc aggcccggca agtcgccgtc gtcgttgaca tgcaggattt ttgcaagttc
     1561 gtcgttttcg atcaccggaa acggcacgac gatctgccga caggaaatcg ggcccggatc
     1621 cagcaggttc tgttcaggcc cgatcgcgcc agcgagcgac gtgacgagct cctcgcggat
     1681 cgagtcaagc ggcgggttgg tgacctgcgc gaacagctgg ctgaaatagt cgaacagcag
     1741 ccgcggccgc acggacaatg ctgcgagtgg gctgtcgttg cccatcgagc caatcggttc
     1801 tgcaccggta ctcgccatcg gggcaagaag gattcgcagc tcctcctcgg tgtagccgaa
     1861 cgtctgctga cggcgcagca cggactcgtg ggaatagctg atgtgctcgc ggtcgggcag
     1921 cttcggcagc ttcaacaggc cggcatgcaa ccagtcgtcg tacggatgct cggccgcgag
     1981 ctgcgacttg atctccgcgt cgtcgacgat ccgaccagcg gccgtatcga cgagaaacat
     2041 gcggccaggc tgcaaacggc ccttgcgtac gatcgaggca ggatcgatgt cgagcacacc
     2101 gacttcactt gctaacacga ccagacccgt gtcggtcacc cagtagcggg cgggacgaag
     2161 accgttgcga tcgagcaccg cgccgatcag agtgccatcg gtaaaggtca cgcaggctgg
     2221 gccgtcccac ggttccatca cactcgcatg gaattcgtag aacgcccgcc gcgcggcgtc
     2281 catctcggtg ttcttctccc atgcctcggg gatcatcatc aaaacggcgt gcggaag
//
LOCUS       DATCBJ010000201         3014 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_467781, whole genome shotgun sequence.
ACCESSION   DATCBJ010000201 DATCBJ010000000
VERSION     DATCBJ010000201.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 3014)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3014)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3014
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_467781"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(293..1243)
                     /locus_tag="VII50_04075"
     CDS             complement(293..1243)
                     /locus_tag="VII50_04075"
                     /inference="COORDINATES: protein motif:HMM:NF020039.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HEY5050054.1"
                     /translation="MGNTGDVVVGTGWKVGCPMGRVESPRVVLDGLAFVESARWHQDR
                     FWFAHWRVGEIIATDLQGNSEVMAPGRPTMGWSFDWLPDGRLLITGESLLRQETDGTL
                     VNHADLSTVARHNWNEIVVDHQRGNVYVNGADTSNFLAGQPPPPGIVAVVAADGAVRQ
                     VADDIDFPNGMVITPDGSTLVVAESMKGRLTAFTITTDGSLIDRRIWADHVAPDGICV
                     DAEGAIWCGAGDVSAPVGQGTSPPGAAVRVRDGGQILDRIELDRPGFSYALGGPTGTT
                     LFIVGQHWRGFDQVDAVIAERTGQAVCVDVATPAPPAATR"
     gene            complement(1422..1988)
                     /locus_tag="VII50_04080"
     CDS             complement(1422..1988)
                     /locus_tag="VII50_04080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5050055.1"
                     /translation="MMSDHDPNDAVVVRASARAQLPAFIAIGLFACGYAVGGLTGVMG
                     AGLAVAGVVIFGGVLIVGAVGLRRIRSSPWEVRLEQEGVTVRGHEVVPWADFTEVRVT
                     GMRPQWIFWASKLVGYRVVAFIATPGVVVPPLPSSSYRGSFERRAGKVRERWYGSRLV
                     IMPRTTNVSIAAITAAVQGFSEVPITYP"
     gene            complement(2152..2544)
                     /locus_tag="VII50_04085"
     CDS             complement(2152..2544)
                     /locus_tag="VII50_04085"
                     /inference="COORDINATES: protein motif:HMM:NF039368.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6176 family protein"
                     /protein_id="HEY5050056.1"
                     /translation="MTEPPLTIPAGLRVELSRAKVKPGMGDEADRWMKMLNDRADECV
                     ASLDRERVAIEIVFRLNEDGVDYLYWMTVAGHGGEGLDLTIPIDRDHNEIGGRVKEPG
                     WVEAEPQVLLLPAPVRAAVMQWALRPAN"
     gene            complement(2716..>3014)
                     /locus_tag="VII50_04090"
     CDS             complement(2716..>3014)
                     /locus_tag="VII50_04090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5050057.1"
                     /translation="DSIATQSRLQFVQAPNFVDPTPPTTLRDGLTGYFHQLYAQGDRL
                     KFVSVTTGDIDSFGDRDIAYQLIHITPTGQQQPAGGTASIECYAGKISALYLTW"
BASE COUNT          505 a          969 c          996 g          544 t
ORIGIN      
        1 gggcaagtcg ccgaagagtt cggtgaggac cctgcacggt ggcggctgct catgttgccc
       61 ttcgggatac ttgggccaat cgtcgccagg gcgttactta gccgaaacgg cggcggccgt
      121 agcggcttct cctaacgctt gagcgaaaag cgcggacagt tccaccgtca ctacacgagt
      181 gtgcgccaag ccggagcaat acgaaccgtt cgacggagca tccgataacg ctgcggcgac
      241 gttagttttc ctgccctgac aggcttctcg tcgtctaggg cattggggtc ggctatcgcg
      301 ttgcggctgg cggcgccggc gtagcgacgt cgacgcagac ggcttgcccg gtccgctccg
      361 cgatgaccgc atcgacttgg tcgaagcccc gccagtgctg gccgacgatg aaaagcgtgg
      421 tcccggtggg accgcccaga gcgtaggaga aacctggccg gtccagctcg atgcggtcga
      481 ggatctgccc gccgtcgcgc acccgtaccg cggcgcccgg cggcgacgtt ccctgcccga
      541 ccggtgcact gacgtcgcca gcgccgcacc agatggcacc ctcggcgtcg acgcagatgc
      601 cgtcgggagc gacgtggtct gcccagatgc gtcggtcgat cagtgagcca tcagtggtga
      661 tcgtgaacgc ggtcaagcgt cccttcatcg attcggcaac gaccagcgtc gagccgtccg
      721 gggtgatgac catgccattg ggaaagtcga tgtcgtcagc tacttggcgg acggcaccgt
      781 cggccgcaac cacggcgacg atccccggcg gcggcggctg acccgccagg aagttggagg
      841 tgtcggcacc gttgacatag acgttgccgc gctggtgatc gacgacgatc tcgttccagt
      901 tgtgccgcgc aaccgtactc aggtccgcat ggttaaccag tgtcccgtcg gtctcctgcc
      961 gtagcaggga ctcgccggtg atcagcaggc gcccgtccgg cagccagtcg aacgaccagc
     1021 ccatcgttgg gcgtccgggc gccatcacct cactgtttcc ctggaggtct gtcgcgatga
     1081 tctcgcccac ccgccaatgg gcgaaccaga acctgtcctg atgccaacga gccgactcca
     1141 cgaacgccaa cccgtcaagc actacccgcg gactctcgac ccgccccatc gggcatccca
     1201 ccttccagcc cgtaccaacg acgacatcac cggtgttccc cacgaacgca aaatccatcc
     1261 gcttctgccg cgattacgtg cgttcgaacc tgccgaatgg cggatgctcc gcggccggct
     1321 ttgcgacgtt ggagaaacta cgggccaagc ccgaatggcg atcgatgccg cgctgccagt
     1381 gcggtgtgat tggctcgtcg acatcgcctc cggcggagcg gttacggata ggtgatcggg
     1441 acttcgctaa acccttggac cgcggctgtg atggccgcga tcgacacatt cgtcgtgcgc
     1501 ggcatgatga cgagacggct cccgtaccag cgttcccgta ccttgccggc ccgccgttcg
     1561 aaagagcccc tgtagctgga agaaggcaga gggggcacca ccacaccggg cgtcgcgatg
     1621 aacgcgacca ctcggtaccc gacgagcttc gacgcccaga agatccactg cggccgcatg
     1681 ccggtgaccc gtacctcggt gaagtccgcc cacggcacga cttcgtgacc gcggacggtg
     1741 actccttctt gttccaaccg aacttcccag ggagagctcc ggatcctccg caggcccaca
     1801 gcgcccacga tcagcacgcc accgaagatg acgaccccgg ctactgccaa cccggccccc
     1861 atcacgccgg tgagtccccc cacggcgtac ccgcacgcaa agagtccgat tgcgatgaag
     1921 gcgggcagct gcgcccgcgc agacgcgcga acaacgacag cgtcgttggg gtcatggtcc
     1981 gacatcacct tgaactaccc ggtcgagatg cttgcgatgc gcatctcacg gcgccgatct
     2041 cgaccgcgca catatgccgc gatgacgcgc gtccgaacgt gccgagatcc ggtagttcag
     2101 ttggggctgc tctgcgggcg tccggtgaga aagcgtctgc ggtgtgcggt gtcagttggc
     2161 ggggcgcagt gcccattgca tgacggcggc gcggaccggc gccggcagca gaagtacttg
     2221 cggttcggcc tccacccagc cgggctcttt tacgcgtccg ccgatctcgt tgtggtcgcg
     2281 gtcgatcggg atggtcaggt cgaggccttc gccgccatgg ccggcgacgg tcatccaata
     2341 caggtagtcg acgccgtctt cgttgaggcg gaagacgatc tcgatggcga cgcgttcgcg
     2401 gtctagggat gcgacgcact cgtcggctcg gtcgttgagc atcttcatcc agcggtcggc
     2461 ctcatcgccc attcccggct tgactttggc gcgggacagt tcgactcgaa gaccagctgg
     2521 aatcgtgagg ggaggctcgg tcatgcttcg atcctggcga atcgaatacg gcctggcaaa
     2581 gcgatggccc aaggctgcgt cttgcgcctg tgccgccgtc ggcatctcct gccgcgatga
     2641 cgcgcgtgcg tgacgttgtc ggcggtgttt gttcgccgag gcgccgttcg cgcgctctcg
     2701 gtcttgctgg ggttgttacc aggttaggta gagggcggag atcttgcctg cgtagcactc
     2761 gatcgaggcg gtgccgccgg ctggttgttg ttggccggtg ggcgtgatgt ggatgagttg
     2821 gtaggcgatg tcgcggtcgc cgaaggagtc gatgtcgccg gtggtcacgg acacaaactt
     2881 caggcggtcg ccttgggcgt agagctggtg gaagtagccg gttaggccgt cccgaagcgt
     2941 cgtcggcggg gtcgggtcga cgaagttggg ggcttgcacg aactgcaagc gtgattgggt
     3001 tgcgatcgag tcgt
//
LOCUS       DATCBJ010000202         3641 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_227878, whole genome shotgun sequence.
ACCESSION   DATCBJ010000202 DATCBJ010000000
VERSION     DATCBJ010000202.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 3641)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3641)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3641
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_227878"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     regulatory      complement(<1..134)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00379"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-AMP (ydaO/yuaA leader) riboswitch senses
                     the signaling molecule cyclic di-AMP; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="cyclic di-AMP"
                     /db_xref="RFAM:RF00379"
     gene            405..794
                     /locus_tag="VII50_04095"
     CDS             405..794
                     /locus_tag="VII50_04095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009073914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEY5050058.1"
                     /translation="MPAPPDALGTAEILQAARARLDRVTPAQAYAQVRNGAVLVDIRP
                     AAQRQPEGEIPGAIVIERNVLEWRLDPRSDHWLPIASYALRVIVVCSEGYTSSLAAAS
                     LLDLGISEATDLDGGFQAWAAAGLPVS"
     gene            863..2230
                     /locus_tag="VII50_04100"
     CDS             863..2230
                     /locus_tag="VII50_04100"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY5050059.1"
                     /translation="MEQVDVVVIGMGPGGEDLAGRLAETRLDVIGIDQRLVGGECPYF
                     GCIPSKMMIRAANALAEGRRIPGLAGDSRVMADWAPVAARIRNEATDDWNDQVAVDRF
                     EGKGGRFVRGHGRLTGSKTVAVGDREFEARRAIVLNTGTEPMIPPIPGLAGTPLWTNR
                     EAIQATLVPASLIVLGGGAIGVELAQVFARFGSHVTVVEAAAQLVPLEEPDAGALLAE
                     VFAREGITVRVGVSAAGIRHDGTGFSVDLADGSTLHAEQLLVATGRRVDLGAVGLDTV
                     GVDITGHAVPVDARMRVAPGIWAIGDMTGKGAFTHVSMYQAEIAIADILGADGVADAE
                     YDALPRVTFTDPEVGSVGMTQAQATQAGIDVLVGRAALPSTARGWIHGPGNDGLIRLI
                     ADADRRVLVGATVVGPSGGEVLSALVVAVHAAVPLDRLRSMIYAYPTFHRGILDAVKD
                     LRRPA"
     gene            complement(2235..2831)
                     /gene="lepB"
                     /locus_tag="VII50_04105"
     CDS             complement(2235..2831)
                     /gene="lepB"
                     /locus_tag="VII50_04105"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:TIGR02227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HEY5050060.1"
                     /translation="MTAESAPENDSAAKPKKSHWGREWVILIVLTVVGTFVLRTYVVQ
                     SYRIPSGSMEKTLHGCTPGCDDDRVLVDKLSYKLHGIHRRDVVVFKATGNWIAVVGGP
                     EDIIKRVIGLPGDTVQCCDSQERVEVNGIPQNEPYIYQNNDYAFGPVVVPKGQLFVMG
                     DHRSDSSDSRYNGTIPESSVIGHAFIRIWPIPRIRTLH"
     gene            complement(2865..>3641)
                     /locus_tag="VII50_04110"
     CDS             complement(2865..>3641)
                     /locus_tag="VII50_04110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5050061.1"
                     /translation="KPELLVALTDFHVLQGFRPAAEAAATLSALQVDGLDPLIQALEG
                     GTPTGEVFLRLIEWPAGDCSRIVAEVSAACRARATDGVAPWIARLAAAYPGDPGVVGV
                     LLLNYLTLHPLEGLYVRPGQIHAYLHGTGIEVLGGSDNVIRGGLTPKHVSAVELRAIL
                     SVDAAAPAVVAPVASSGGGQSWPTPQPEFALHREHIGALERLVPGGPSVLLCLEGKLE
                     IGDRDGSVTLGPGESAFAFGGADLAVAGSGVLVRANPGTG"
BASE COUNT          612 a         1214 c         1234 g          581 t
ORIGIN      
        1 ccctcgaccg cctaccgggt tagctgacgg attcgggcgg gaagtgcgcc ctacggcccg
       61 aaggccgatt caccccacgt tcgcaaacct ttggttcccc ggccccgctt gcgcgggttc
      121 ggcggtggtc ggttacgtca cggacagtga cgcaacccaa ccgcaacgac cctaactggg
      181 gttggagatg gtcacaagct gaagtccgca gttcatcccg gttcaggacc tcattcaccc
      241 agaaggacta gcggtggcta ggagtcgacc agcccgttac gtacccgttc cgcccagtac
      301 gcggtcggag taggtggtca cgctggaagg acatcgccgc cctcgacctt gatcgagatt
      361 gccgcgagcg gcagcggagc cggaccgctg atgacggcgc aacggtgcct gcacctcctg
      421 atgcgctcgg caccgcggag atcctgcaag ctgcgcgggc gcgattggat cgcgtcacac
      481 cggcccaggc ctacgcgcaa gtccggaacg gcgcggtact cgtcgacatc aggccggctg
      541 cgcaacgaca gcccgaaggt gagatcccgg gcgcgatcgt gatcgagcgc aacgtgcttg
      601 agtggcggct cgacccgcgc agcgatcact ggctgccgat cgctagctat gcgctacgcg
      661 tcatcgtggt gtgctccgag ggctacacct cgagcctggc tgccgcgagc ctgctcgacc
      721 tcggaatctc cgaagcgacg gacctcgacg gcggattcca agcctgggcg gcggcaggcc
      781 tacccgtcag ctagcgacct cgatccgcca ggcaccacaa tcgggtgatc gattcgatca
      841 cttggaggcc tggggaggca ccgtggagca ggtcgacgtc gtcgtcatcg ggatgggtcc
      901 tggcggggaa gatctcgcgg ggcgactagc cgaaacgcgc ctcgacgtga tcggcattga
      961 tcagcggctg gtcggaggcg agtgtccgta ctttggctgc atcccgagca agatgatgat
     1021 ccgggccgcg aacgccctcg ccgaaggccg gcggattccg gggctcgccg gcgactcgcg
     1081 agtcatggcc gactgggcac cggtcgccgc gcgaatcagg aatgaggcca ccgacgactg
     1141 gaacgaccag gtcgccgtcg atcggttcga gggcaagggt ggccggttcg tccgcggaca
     1201 cggccggctg accgggtcga agacggtcgc cgtcggtgat cgcgagttcg aagcgcgacg
     1261 cgcgatcgtg ctgaacaccg gcaccgagcc gatgatcccg ccgatccctg gcctggccgg
     1321 aacgccgctc tggacaaacc gcgaggccat ccaggcgacc ctggtaccgg cgtccctcat
     1381 cgtgctcggc ggcggcgcca tcggcgtcga gctggcccag gtattcgccc gattcggttc
     1441 gcacgtgacg gtcgtcgagg ccgcggcgca gctagttccg ctcgaggagc cggacgccgg
     1501 ggcgttgctc gccgaggtct tcgcgcgtga gggaatcacc gtgcgggtcg gggtgtcagc
     1561 tgccgggatc cggcacgacg gcaccggctt tagcgtcgac ctcgccgacg gcagcacctt
     1621 gcacgcagag caactgctcg tcgcaacggg ccgccgagtc gacctcggcg ccgtcggcct
     1681 cgacacggtc ggcgtcgata tcactgggca cgccgtaccg gtcgatgcac gcatgcgggt
     1741 cgcacccggc atctgggcca tcggcgacat gactggcaag ggcgcgttca cccacgtgtc
     1801 gatgtaccag gcggagatcg cgatcgccga catccttggc gccgatggtg tcgccgatgc
     1861 ggagtacgac gcgctgccga gggtgacgtt caccgatccc gaggtgggta gcgtcgggat
     1921 gacccaggcg caggcgacgc aggccgggat cgacgtcctc gtcgggcgtg ctgcgctgcc
     1981 gtcaacggcg cgcggctgga tccatggccc cggcaacgac ggccttatcc ggctgattgc
     2041 cgacgcggac cgacgcgtct tggtcggcgc gaccgttgtc gggccgtcag gtggtgaggt
     2101 gctgtccgcg ctcgtggtcg cggtccatgc agccgttcca ctcgaccgcc tgcggtcgat
     2161 gatctatgcg tatccgacgt tccaccgcgg catcctcgac gccgtgaagg acctgcgccg
     2221 gcccgcctag aaagctagtg cagggtgcgg attcgcggga tcggccagat cctgatgaac
     2281 gcgtggccaa tcacgctgct ctccggaatc gtgccgttgt agcgcgagtc cgaggaatcc
     2341 gatcgatggt cgcccatcac gaagagctgc cctttaggca ccacgaccgg accgaacgca
     2401 tagtcgttgt tctggtagat gtacggctcg ttttggggga tgccgttgac ctcgacgcgt
     2461 tcctgcgagt cgcagcactg cacggtgtca ccgggcaacc cgatcacgcg tttgatgatg
     2521 tcttcgggac cgccgacgac cgcgatccag ttgccggtgg ctttgaacac gacgacgtcg
     2581 cggcggtgga tgccgtgcaa cttgtaggag agcttgtcga caagaacccg gtcgtcgtca
     2641 caccccggtg tgcagccgtg caacgtcttc tccatcgatc cggacgggat ccggtacgac
     2701 tggacgacgt aagtgcgcag cacgaacgtc ccgaccacgg tcaacacgat gaggatgacc
     2761 cactcgcggc cccaatggga tttcttgggc ttcgccgccg aatcgttctc cggcgctgac
     2821 tcggccgtca ccacgcgatc tcgcggttgc acagcgagcg cacgtcagcc ggtcccaggg
     2881 ttggcgcgga ccaacacccc gctaccagca acggccaagt cggcgccccc gaacgcgaaa
     2941 gccgactcac ctggacccaa ggtgacactg ccgtcgcggt cgccgatctc gagcttgccc
     3001 tcaaggcaga gcagcactga cgggccaccg ggcaccaggc gttccagcgc gccgatgtgc
     3061 tcccggtgca acgcgaactc cggctgcggc gtcggccatg actgcccgcc gccgctcgac
     3121 gcaactgggg cgaccacggc gggcgcggcg gcatcgacgc tcaggatcgc gcgcaactcg
     3181 acggcggaca cgtgtttggg ggtcagaccg ccgcggatca cgttgtctga gcctccgagt
     3241 acctcgatgc cggtgccgtg cagatacgcg tggatctgtc ctggtcgaac atacagaccc
     3301 tccagcgggt gcagcgtcag gtagttcagg agcaggacac cgacgacgcc gggatcgccg
     3361 ggatacgcgg cggccaaccg ggcaatccat ggggccacgc catcggtggc acgagcgcga
     3421 caggccgcgc tcacttcggc aacgatccgg ctgcagtcgc cggccggcca ctcgatgagc
     3481 cggaggaaga cctcgcccgt cggcgtcccg ccttcaaggg cttggatcaa cggatcgagg
     3541 ccgtcaacct gcaatgccga cagagtcgca gcggcttccg cggccgggcg gaacccttgc
     3601 agcacgtgga aatctgtcag cgccacgagc agttcgggct t
//
LOCUS       DATCBJ010000203         3573 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_22828, whole genome shotgun sequence.
ACCESSION   DATCBJ010000203 DATCBJ010000000
VERSION     DATCBJ010000203.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 3573)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3573)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3573
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_22828"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1567
                     /locus_tag="VII50_04115"
     CDS             <1..1567
                     /locus_tag="VII50_04115"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEY5050062.1"
                     /translation="RIGVLAQGDALDPTTTVRHVVLGDRPDHSWAGDRVARTVVSTLL
                     STDMLDRVVGTLSGGERRRTALAALLVTDIDLLMLDEPTNHLDIETIDWLAGFLASRR
                     GALVVVTHDRWFLDAVCTLTWEVADGQIAQYLGGYAAYVLARAERERTSGVVEERRQN
                     LLRKELAWLRRGPPARTSKPQFRIDAANALIADEPPARDRTVLATFSSARLGKSVFDV
                     EDISVSLGSGLLFSDVTWRVGPGDRIGLVGVNGSGKTTLLRILAGLRGIDSGVVKVGK
                     TVRIGYLSQDVAELDPTLRVLEAVEAVRRQTVLGDGREVTASALLERFGFTGERQWTP
                     VGELSGGEKRRLQLLRLLMDEPNVLLLDEPTNDLDVDTLTALEDLLDNWAGTLIAASH
                     DRYFLERVCDSVTALIGGGQLASLPGGVDEYLARRIRERAAVLGALAPVAAAVSAGTV
                     GEVRAARKDVTRIERQLSKLADREKQLHDQLAAAATDYVAAAALDSELRALHVARAQL
                     EEDWLDAAHRAGH"
     gene            complement(1589..1930)
                     /locus_tag="VII50_04120"
     CDS             complement(1589..1930)
                     /locus_tag="VII50_04120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5050063.1"
                     /translation="MSLSHFTNVTATTPAGMNVIGVYAISYADGLPPALSSGSGSDWT
                     RLGYSKLPPHKVSEVTVDPRSSKNLWWVVLKVVPNRPGPLASTGLRVSYTAGGRQGVT
                     TYPYGMTMTCQ"
     gene            complement(2166..2594)
                     /locus_tag="VII50_04125"
     CDS             complement(2166..2594)
                     /locus_tag="VII50_04125"
                     /inference="COORDINATES: protein motif:HMM:NF024212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEY5050064.1"
                     /translation="MDDTGRTSRVIRLARHLELARRDVLAFRELDIWEYDVLAALRAA
                     GAPHELSPGALVDETQVASGTMTNRIDRLSRRGYVTREPDPADRRGVLVRLTVSGRKR
                     IDLVTADLATAEALAWQRLGARKTDQLDSVVTELISHLES"
     gene            2721..3110
                     /locus_tag="VII50_04130"
     CDS             2721..3110
                     /locus_tag="VII50_04130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020660999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY5050065.1"
                     /translation="MIARIDHLVLTVQSLDATCDFYTTCLDFRRVDSPGRPTALLFGQ
                     QKFNVHQADHTFDPKARTPTPGSADFCLVADVRLTELVDRLAQHQVAIELGPVEREGA
                     RGPMRSIYFRDPDGNLVEVSQYRSEVF"
     gene            3172..>3573
                     /locus_tag="VII50_04135"
     CDS             3172..>3573
                     /locus_tag="VII50_04135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5050066.1"
                     /translation="MGSLPAGLWKPFIRLDADRIRLHATSAPDTFAGEVAARVAFVRD
                     ALFPATEARRARNPILVAACYLLAAVAGFTVDLWWSGSGITSRVWAEDGTIFLSTAYR
                     MPYLSALAHPYAGYLQVLPRSLAAFTTAFPVR"
BASE COUNT          557 a         1162 c         1200 g          654 t
ORIGIN      
        1 gcggatcggt gtactggctc agggggacgc gcttgaccct acgacgacgg tccgccacgt
       61 cgtcctcggt gatcgtcccg accactcgtg ggccggcgat cgcgttgcgc gaaccgtggt
      121 gtcgacgttg ctgtcgaccg acatgctcga ccgtgtcgtt ggcaccctgt cgggtggcga
      181 gaggcggcgt accgcgcttg ctgcgctact cgtgaccgac atcgacctgc tgatgctcga
      241 cgagccgacc aaccacctcg acatcgagac gatcgattgg ctggctgggt tcttggcgtc
      301 gcgtcgaggc gcgcttgttg tcgtgaccca cgaccgctgg ttcctcgacg ctgtctgcac
      361 gttgacgtgg gaagttgccg acggtcagat cgcgcagtat ctcggcgggt acgcggcata
      421 cgtgttggcg cgcgccgaac gcgagcgcac ttcgggagtg gtcgaggagc gccggcagaa
      481 cctgctgcgc aaggagttgg cctggctgcg gcgcggaccg cccgcgcgca cctcgaagcc
      541 gcagttccgt atcgatgccg cgaacgcact gatcgccgac gagccaccgg cacgtgatcg
      601 aaccgtgttg gccacgtttt cctcggcccg gttggggaaa tcggtgttcg acgtcgagga
      661 catatcggtg tcgcttggat ctggtttgtt gttctccgac gtcacgtggc gggtgggtcc
      721 gggcgaccgg atcggcctcg tcggcgtgaa tggcagcggg aagacaacgc tgctgcgcat
      781 tctcgccgga ctccggggga ttgacagcgg cgtcgttaag gtcgggaaaa ctgtgcgcat
      841 cggctacctg tcgcaagacg tcgccgagct ggacccgaca ttgcgggtgc tcgaagccgt
      901 cgaggcagtg cgccggcaga cggttctcgg cgacgggcgc gaggtcacgg cgtccgcgct
      961 gctggagcgg tttggcttca ctggcgagcg acagtggact ccggtcggtg agctttccgg
     1021 cggggagaag cggcggctgc agttgctgcg gttgttgatg gacgagccga acgtgctgct
     1081 gctcgacgaa cccacgaacg acctcgacgt cgacaccctc acggctttgg aggacctgct
     1141 cgacaactgg gctggcacgc tgatcgccgc gtcgcacgat cggtatttcc tcgagcgggt
     1201 ctgtgattcg gtcacggcat tgatcggtgg cgggcagctc gcttcgttgc ccggcggagt
     1261 agacgagtac ctagcccgtc gcatccgcga acgcgccgcg gttcttggtg ctctcgctcc
     1321 cgtcgccgcg gctgtcagcg cggggactgt gggcgaggtg cgagccgctc gcaaggacgt
     1381 gacccgcatc gaacgccaac tgtccaagtt ggccgaccgc gagaagcagc tgcacgacca
     1441 gctcgcggca gccgcgaccg actacgtggc cgccgcagca ctcgactccg agttgcgcgc
     1501 gctacacgtt gcccgcgccc aactcgaaga ggactggcta gacgccgccc accgcgccgg
     1561 ccactgagtg ggtcgtctcg ctgggacgct attggcaggt catggtcatg ccgtacgggt
     1621 aggtcgtgac gccctgacgg ccgcctgccg tgtaggagac tcgcagcccc gtcgaggcca
     1681 ggggacccgg ccgattcggg acgaccttca gcacgaccca ccacaggttc ttggacgacc
     1741 gagggtcgac agtgacctcg ctcaccttgt gtggcggcag tttcgaatac cccagtcgtg
     1801 tccagtcgct cccggagccg cttgacagcg ctggcggcaa gccgtcggca tacgagatcg
     1861 cgtacacgcc aatcacgttc atgccggcgg gcgtcgtggc agtcacgttc gtgaagtgac
     1921 tgaggctcat accacgcgca ccccatccgg tccaaccgaa atactgcggc gcgccaacct
     1981 tgcacgtcgt gggcccttcg gacgatccga aggcgggtgc gacgagctca tgggtgcgcg
     2041 acggccaaaa cgccgtcgca caccagtaat acgacagcta ctgcaccagc cgcgagtcct
     2101 atctttgccc gcaagctctc cccgatccgc tcgccgcggt ggtcggccca gcgccacccg
     2161 cggcgctacg actcgaggtg gcttatcagt tctgtcacga ccgaatcgag ttggtcggtc
     2221 ttgcgggcgc ccagtcgttg ccacgcgagc gcctcggccg tggcgaggtc tgcggtgacc
     2281 aagtcgattc tcttgcggcc gctcacggtg agccgcacca gcacgcctcg ccgatctgcg
     2341 gggtcgggct cgcgggtcac gtagccgcgc cggctcagcc gatcgatgcg gttcgtcatc
     2401 gtgcccgatg cgacctgagt ctcgtccacc agcgcgccgg gtgacagctc gtgcggcgct
     2461 ccggccgctc gcagcgcggc cagcacgtcg tactcccaga tgtcgagctc acggaacgcg
     2521 agtacatcgc ggcgcgcaag ctcgaggtgc cgcgcgagcc tgatgacccg tgatgtccgg
     2581 ccagtgtcgt ccacgcgcgg agtctaccga caaatctcga cgtcgagata ctgcaaaccc
     2641 gcagttcagg accactttcc gcgagcaaag cgcgcggaag agtgttcggt cgcggagtcg
     2701 ggaacggcta cggttggggc atgatcgctc gcattgacca tcttgtgctc actgtgcagt
     2761 cgctcgatgc tacctgtgac ttctacacga cgtgtctcga ctttcggcgc gtcgacagtc
     2821 cgggccggcc gaccgccctg ctgttcggcc agcagaagtt caacgtgcat caagccgacc
     2881 acacgttcga tccgaaagct cgaacgccca caccagggtc cgccgacttc tgtctggtcg
     2941 ccgatgttcg gctgaccgag ctggtggatc gactcgcgca gcatcaggtc gcgatcgagc
     3001 tggggccggt cgagcgcgaa ggcgcacgtg gcccgatgcg gtcgatctac ttccgcgacc
     3061 ccgacgggaa cctggtggag gtcagccagt accgcagtga ggtgttctga gagctgacaa
     3121 ctagttgagt ggtcccttgc gtctcacggt ccgacgtgag gggtcactcg tatgggctcg
     3181 ttgccagccg gtctgtggaa gccattcatc cggttggacg ccgaccgcat ccgcctgcac
     3241 gcgacatcgg cgccggatac cttcgcggga gaggtcgccg cacgggttgc gttcgttcgc
     3301 gacgctctgt tcccagcgac tgaggcgcgc cgggcgcgaa acccgatcct cgttgccgcc
     3361 tgttacctgc tggccgcagt tgcgggcttc accgtggact tgtggtggag tggctctggc
     3421 atcacgtcgc gggtgtgggc cgaggacggc acgattttcc ttagtacggc gtatcgaatg
     3481 ccctacctgt cggcactggc acacccgtac gcgggctacc tgcaggtgct gccgcgatcc
     3541 ctagcagcgt tcacgacggc gtttccggtc cgt
//
LOCUS       DATCBJ010000204         2079 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_419720, whole genome shotgun sequence.
ACCESSION   DATCBJ010000204 DATCBJ010000000
VERSION     DATCBJ010000204.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2079)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2079)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2079
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_419720"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..376
                     /gene="rimI"
                     /locus_tag="VII50_04140"
     CDS             <1..376
                     /gene="rimI"
                     /locus_tag="VII50_04140"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: protein motif:HMM:TIGR01575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HEY5050067.1"
                     /translation="PETRFYLVARDGAEIIGYAGLCAYPEESFVQTIAVKRERQGTGI
                     GTRLLTALIDEARRRDEPMVGLEVRADNMVAQRLYQGFGFEQVGIRKGYYQPSDTDAV
                     LMVLNLAGVDVSKWAPTDGSDT"
     gene            376..1413
                     /gene="tsaD"
                     /locus_tag="VII50_04145"
     CDS             376..1413
                     /gene="tsaD"
                     /locus_tag="VII50_04145"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004559933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HEY5050068.1"
                     /translation="MVGVSQALVLGIETSCDETGVGIVHGTTLLANAVASSVEEHARF
                     GGVVPEVASRAHLDAMVPAVHRALADAGIAMRDVDAIAVTSGPGLAGALLVGVAAAKA
                     YALALDKPLYGVNHLSAHVAVDVLEHGPLPEPCVALLVSGGHSSLLLVPDLTREVHSL
                     GSTVDDAAGEAFDKVARVLGLPFPGGPQIDRVALDGDPGLIAFPRGKSDDGSLDFSFS
                     GLKTAVARWVEARERAGEAVPIAHVAASFQEAVVDVLIMKAIAACRAHGVDYLVIGGG
                     VAANSRLRAVASQRCEAAGITLRVPRPGLCTDNGAMVAALGTRLVEAGLPPSDLDLPA
                     DPSLPITEVKV"
     gene            1410..1688
                     /locus_tag="VII50_04150"
     CDS             1410..1688
                     /locus_tag="VII50_04150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5050069.1"
                     /translation="MIARMWETRITEGQLDEFCAWVKTTAWPQFLAAPGFGGGELYRA
                     DEQDRAVIVTRWATTEALATGNEWFDLGAERFVAREPEAWEFTPVDLG"
     gene            complement(1666..>2079)
                     /locus_tag="VII50_04155"
     CDS             complement(1666..>2079)
                     /locus_tag="VII50_04155"
                     /inference="COORDINATES: protein motif:HMM:NF037043.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5050070.1"
                     /translation="LDEPAPTDAMRHYLYEPDDAVVRAHLIGDLARAIDGVLLDPTTA
                     YLTSDRLVETPFARAFEVHDVMPFSLKRLRSALRERDIGGVTIMKRGSAVNVEDLRRD
                     LRLSGAKQAVVVLALIDGRHHAVIAEDVLSRGRRG"
BASE COUNT          319 a          656 c          731 g          373 t
ORIGIN      
        1 gccggaaacg aggttctatc tcgtcgcacg tgacggcgct gagatcatcg gatacgccgg
       61 actttgtgcg taccccgaag agtcgttcgt gcagacgatc gcggtgaagc gtgagcgcca
      121 gggcaccggg atcggcacca ggctcttgac ggcgctcatc gatgaggcgc gccgccgtga
      181 cgaaccgatg gtcgggctgg aggtgcgcgc cgacaacatg gtcgcgcaac ggttgtatca
      241 agggttcggc tttgagcagg ttggtatccg caagggctac taccagccga gcgatacgga
      301 cgcagtgttg atggtgctca acctcgcggg cgtcgacgtg tcgaagtggg cgccgaccga
      361 cggttccgat acgtgatggt cggagtttcg caagcgctgg tgctcggcat cgagacgtcg
      421 tgcgacgaga caggcgtcgg cattgtgcac ggcaccacgt tgctcgccaa cgcagtcgcc
      481 tccagcgtcg aggagcacgc acgcttcggc ggtgttgttc cggaggtcgc aagccgcgcg
      541 catctcgacg cgatggtgcc cgcggtgcat cgcgcgctgg cggacgccgg gatcgcgatg
      601 cgcgacgtcg acgccattgc cgtgacgtcg ggtcccggac ttgccggcgc cttgctcgtc
      661 ggtgttgccg ccgcaaaggc ctacgcgctc gccctcgaca agcccctcta cggcgtcaac
      721 catctgtcgg cccacgtcgc ggtcgacgtt ctcgagcatg gaccgctgcc cgaaccgtgc
      781 gtggcgctgc tggtgtcggg cggccactcc tccttgctac tcgttccgga cctcactcgc
      841 gaggtgcact cgctcgggtc gacagtcgac gacgcggccg gcgaggcgtt cgacaaggtc
      901 gcccgcgtgc tcgggttgcc gttccccggc ggcccgcaga tcgaccgggt ggcgctggac
      961 ggcgaccctg gcctgatcgc gtttccgcgg ggcaagtccg acgatggcag cttggatttc
     1021 tccttctcgg ggctcaagac cgcggttgcc cgctgggtcg aggcgcgcga gcgagccggc
     1081 gaggcggtgc caatagccca tgtggccgca tcgttccagg aggccgtggt cgatgtgttg
     1141 atcatgaagg ccatcgctgc gtgccgggct cacggcgtcg attacctcgt gatcggtggc
     1201 ggtgtggcgg ctaactcgag gcttcgcgcg gtggcctcgc agcgctgcga agcagccggt
     1261 atcaccctgc gggtaccacg gccgggttta tgcactgaca acggtgcgat ggtggctgca
     1321 ctgggtactc gactagtaga ggccggcctg ccgccgtccg atctggatct accggccgat
     1381 ccgtcgctgc ctattacgga ggtgaaggtg tgatcgcacg catgtgggag acccggataa
     1441 cggagggtca gctcgacgag ttttgcgcgt gggtgaagac cacggcctgg ccgcagttct
     1501 tggcagctcc aggctttggc ggtggcgagc tctaccgcgc cgacgaacag gaccgtgcag
     1561 tcatcgtcac gcggtgggcc acgactgagg ccttggcgac cggcaacgaa tggttcgacc
     1621 tcggcgcaga acgcttcgtc gcgcgcgagc cggaagcctg ggagttcacc cccgtcgacc
     1681 tcggctgagc acgtcctcgg cgatcacggc gtggtgccgt ccatcgatga gcgcaaggac
     1741 taccacggct tgctttgctc ccgacaaccg aaggtcacgg cgtaagtctt cgacgttgac
     1801 cgccgagccg cgcttcatga tcgtgacgcc tccgatgtcg cgctcgcgga gtgcgctgcg
     1861 cagccgcttc aacgaaaacg gcatgacgtc gtgtacttcg aaggcgcgcg cgaacggcgt
     1921 ctcgaccagc cgatcgctcg tgaggtaggc ggtggtcggg tcgagcagga cgccgtcgat
     1981 cgctcgtgcg agatcgccga tcaggtgcgc acggacgacg gcatcgtcgg gctcgtacag
     2041 gtagtgccgc atcgcatccg tcggcgccgg ctcgtcgag
//
LOCUS       DATCBJ010000205         3347 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_103672, whole genome shotgun sequence.
ACCESSION   DATCBJ010000205 DATCBJ010000000
VERSION     DATCBJ010000205.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 3347)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3347)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3347
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_103672"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1424)
                     /locus_tag="VII50_04160"
     CDS             complement(<1..1424)
                     /locus_tag="VII50_04160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exonuclease H3TH domain-containing
                     protein"
                     /protein_id="HEY5050071.1"
                     /translation="MPNASGSPVTPLATTVASMKPGDRPRLLLIDGHSVAYRAFFALP
                     VENFSTTTGQPTNAVYGFTSMLINVLRDEKPTHVGVAFDISRQSFRTETFPEYKANRS
                     STPNEFKGQISLIQEVLAALAIPVITAEGFEADDVIATLATQAEADGLDVLICSGDRD
                     VFQLVTAQVTVLYPRKGVSDMARMTPAAVEERYGLTPGQYADFAALRGDPSDNLPNIP
                     GVGEKTAAKWVRDFGSLTALVDRVDEVPGKAGGLLRDHLSAVLRNRQLTELIRDVPLP
                     VGPHDLNLGQWDREAVHRVFDSLQFRVLRERLYATLSAPEPEADAGFTVEGGQLGADE
                     VAAWLAEHSAPGVRLGVSVAGSWGRGTGVVTGLALAAPDGTGAYLDPTQLTEADDRAL
                     AAWFADPGQPKALHDAKGPILALAARGWPLAGLSSDTALAAYLALPGQRSFDLGDLAL
                     RYLHRELRADGSGGPVESAQLSLDG"
     gene            complement(1494..2120)
                     /locus_tag="VII50_04165"
     CDS             complement(1494..2120)
                     /locus_tag="VII50_04165"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY5050072.1"
                     /translation="MSRSHITVRAAGESDVADLVVLWGELKRCGALNGRHAAVPDDRT
                     IEDRVRRLLGRPSGRILLAFLGDEPVGMAMLTTVPLGALSEAACVQVEYTVVAEGFRR
                     RGVGRALMGSAACYAEDIGAEQVTVSVSPMSREANRFYAQLGLAPLSVRRVAPASALR
                     RRIATLDHGPAAHDHPTGRRSLGQRAGMRAALRRMAIASAVAEQPNDA"
     gene            complement(2803..>3347)
                     /locus_tag="VII50_04170"
     CDS             complement(2803..>3347)
                     /locus_tag="VII50_04170"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEY5050073.1"
                     /translation="RRWPPRKAGIVVLVALIGLALLVGLPANRSDPGRLLAGAGLAAL
                     SGATFAAFTLLGRRPLVAVDQRTVTGYGFLLGGIILAVVTAPTTGLGFAPSLATLGLL
                     AFLAVVPTAIAYGLFFRGLRSVTASNATVIALLEPLTGTLLAIALLGDRLSPAGVVGG
                     VLLGLSIVASIRTSIPPVGL"
BASE COUNT          633 a         1124 c         1103 g          487 t
ORIGIN      
        1 ccgtccagcg acaactgcgc ggactcgacc gggccacccg agccgtcggc acgcagctcg
       61 cgatgcaggt agcgcaacgc cagatcgccg agatcgaagg accgttgacc cggcaacgcg
      121 aggtaggcgg cgagcgcggt gtcactgctc agcccggcca gcggccaccc ccgcgcggcg
      181 agcgcgagga tcggcccctt ggcgtcgtgc aacgccttcg gttgaccggg atcggcgaac
      241 cacgctgcca gcgcgcggtc gtcggcctcg gtcagttggg tcggatcgag gtaggcgccg
      301 gtgccatcgg gggcagccaa cgcgaggccg gtgacaacac ctgtgccgcg gccccagctg
      361 cccgcaaccg acacgccgag ccgcacgccc ggcgcactgt gctcggccag ccaggccgcg
      421 acctcgtcgg cgccgagttg gccgccttcg acggtgaatc cggcgtccgc ttcgggttct
      481 ggcgcggaca gggtggcgta cagtcgctcg cgcaagaccc ggaactgcag cgaatcgaag
      541 acccggtgga ctgcttcgcg atcccactgg ccaaggttca gatcgtgcgg gccgacgggt
      601 agcggcacgt cgcgaatcag ctcggtcaac tgccggtttc gcaaaaccgc ggaaaggtgg
      661 tcgcgcaaca ggccgccggc cttgccaggg acctcgtcga cccggtcgac gagggccgtc
      721 agcgacccga agtcgcggac ccattttgcc gcggtcttct cgccgacgcc cggaatgttc
      781 ggcaggttgt cgctcggatc gccgcgcagc gcagcgaaat cggcatactg ccccggcgtc
      841 aggccgtatc gctcctcgac ggccgcgggg gtcatccgcg ccatgtcgga gacgcccttg
      901 cgcggataca gcacagtgac ctgcgcggtc acgagttgga agacatcgcg gtcaccggag
      961 cagatcagga cgtcgaggcc gtcggcctct gcctgcgtcg ccagcgtcgc aatgacgtca
     1021 tcggcctcaa agccctcagc ggtgatgacc ggaattgcca gcgccgcaag gacttcttgg
     1081 atcaggctga tctgcccctt gaactcgttc ggcgtcgacg accggttcgc cttgtactcg
     1141 gggaaagtct cggtacgaaa actctgccgc gaaatatcga aggcaacccc gacatgcgtc
     1201 ggcttctcgt cccgcaagac attgatcaac atcgacgtga acccgtagac cgcgttcgtc
     1261 ggctggccgg tcgttgtgga gaaattctca acaggcaaag caaagaacgc gcggtacgcg
     1321 acggaatgcc cgtcaatcag caacaggcgg ggtcggtcac caggcttcat cgaggcgaca
     1381 gtggttgcca gcggcgttac aggcgatccg gaagcgttgg gcaccctgtg atcctaggca
     1441 ccgccaaaga caggcacagc caggcacgac atcccggtcg agcagccgac cggtcaggcg
     1501 tcgttcggct gctcggccac tgcggacgcg atcgccatgc ggcgcagtgc ggcccgcatc
     1561 ccggcacgtt ggccaagcga ccggcgtcca gttgggtggt cgtgcgcagc aggaccgtgg
     1621 tcgagggtcg cgatccgtcg ccgcaaggcc gacgccggag cgacccggcg aaccgacaac
     1681 ggcgcgagac caagctgggc gtagaaccgg ttggcttcac gcgacatcgg agacacgctg
     1741 acggtgacct gctccgcacc gatgtcctcg gcgtagcacg cagccgagcc catcaaggcg
     1801 cgaccgacgc cgcgacggcg gaagccctcg gcgaccaccg tgtactcgac ctggacgcac
     1861 gcggcctcac tcagcgcacc cagcggaacc gtggtcagca tcgccatgcc gacgggctca
     1921 tcgccaagga aagcgagcag gatgcgaccg ctcgggcggc ccagcaaccg gcgtacccgg
     1981 tcttcgatcg tgcggtcatc gggaacggcc gcgtgccggc cattcaaggc accgcatcgc
     2041 ttgagctcgc cccacagcac cacgaggtcg gcaacatcgg actcgccggc agctcgcacc
     2101 gtgatgtgag aacggctcat cctgtgctcc tcagtgcggc cgcgtgggcc gcctcgagtt
     2161 cactcccccg ggttggaaaa actgtgcctg aagcgtggcc gaactgtgat caaactgttc
     2221 ctcgaacggc accggcggca acgccacctg ctgttcaccc ggagatcggg agcgtagcga
     2281 ggaatggcag ggatgtgcag cccttccgag aagaaagatc aaccaccgag ccgagaacgg
     2341 cgtgtctgcg cttttcaccg cagtcacgac aagtagccca aggcagcgtt gcccggcgtc
     2401 ggcgttttca tctgcttggt tggtggatgt cgtattccgc gatcacattc cgaaagcgac
     2461 ctactacagc ttgtgttcac cagctgaatc ggcgcgcgaa aaatttctca ccagcctcac
     2521 cgataccaag ccgctgcggt cccgatcaca tcggccggag caacggtgcc tttccatcgg
     2581 tgaggtaaac gaaggaccac gcacagggaa aggaaccgtt acggcgttcg gcacctgacg
     2641 tttctgcggg caatcgcggg gatgctttgc cgtgaccgtc aagacctcgg tacggtgtgt
     2701 cccgacgagc ccgggtgcct agtagagaac gtccaagacc ggggaaggct tggacggcgc
     2761 caacagtgcc cgggctcgct cgccgcattt ttgacccgcg gcctacaaac ccaccggcgg
     2821 gatcgacgtc cggatcgaag caacgatcga gagaccgagc aacacgccac ctaccacgcc
     2881 tgccggggac aacctgtcgc ctagcaacgc gatcgccagc aacgtgcccg tgagcggttc
     2941 cagcagagcg atgaccgtcg cgtttgatgc cgtcaccgac cgcagtccgc gaaagaacag
     3001 gccgtaggcg atagccgtgg gcacgacggc caggaacgcg agcaatccga gggtggccag
     3061 cgacggtgcg aacccgagcc cggtggttgg cgccgtcacg actgccagga tgatcccgcc
     3121 gagcaggaat ccgtagccgg tgaccgtgcg ctgatcgact gcgaccaggg gccggcgtcc
     3181 cagcagcgtg aacgcagcga aggtcgcacc cgagagcgcc gcgagtccgg cgccggcgag
     3241 gagccgtcct gggtccgatc gattggccgg cagtccgacg agcagggcca agccgatgag
     3301 cgcgacgagc acgacaatcc cggccttacg cggtggccag cgacgct
//
LOCUS       DATCBJ010000206         2003 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_59181, whole genome shotgun sequence.
ACCESSION   DATCBJ010000206 DATCBJ010000000
VERSION     DATCBJ010000206.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2003)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2003)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2003
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_59181"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..421
                     /locus_tag="VII50_04175"
     CDS             <1..421
                     /locus_tag="VII50_04175"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HEY5050074.1"
                     /translation="DATHQLIVTRNGTVEQTFPMSMGMAAGGHQTPNGTYYVLDKKAK
                     VVMDSSTYGVPVNSTYGYKVDVQDAVQFDNSGDYVHSAPWSVADQGKRDVSHGCINIS
                     PSNAQWFFDNFGSGDPVVVKNSVGTYSKNDGSNDWQL"
     gene            complement(418..>2003)
                     /locus_tag="VII50_04180"
     CDS             complement(418..>2003)
                     /locus_tag="VII50_04180"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF017464.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M13-type metalloendopeptidase"
                     /protein_id="HEY5050075.1"
                     /translation="DTDSKNSTRYLLHLSQSGLGLPDESYYRDPQHAAILAEYPKHIA
                     RTFALVYGEHEGDWDETAAHIVALETKIAAAHWDVVKRRDADLTYNLRTFAELSSEAT
                     GFDWPVWLHALGADLSGQASGPLEVVVHQPGALITFAALWDSEDIEDWRRWLRWRVIS
                     GRASLLSDDIVDENFAFYGRTLSGTEQLRDRWKRGVGLVESLMGDAVGRLYVERHFPP
                     DAKARMDELVANLREAYRVSITDLEWMTPETRQRALEKLDKFTPKIGYPAKWRDYSSL
                     VIARDDLYGNYQRGYALEYDRDLAKLGGPVDRDEWFMTPQTVNAYYNPGMNEIVFPAA
                     ILQPPFFDAEADDAANYGGIGAVIGHEIGHGFDDQGAKYDGDGNLIDWWTDNDRAEFG
                     SRTKALIEQYEVFTPRSLGASHHVNGAFTVGENIGDLGGLSIALLAYQLSLKGAEAPV
                     IDDLTGVQRVFFGWAQVWRTKSRDAEAIRRLAIDPHSPPEFRCNGVIRNIDVFYDAFE
                     LTETDELYLDPQRRVRIWN"
BASE COUNT          349 a          645 c          651 g          358 t
ORIGIN      
        1 cgacgcgact caccagctga tcgtcacgcg caacgggacg gtcgagcaga ccttcccgat
       61 gtcgatgggt atggccgcgg gcggtcacca gacgcctaac ggcacctatt acgtgctcga
      121 caagaaggcc aaggtcgtga tggattcgtc cacctacggt gtgccggtca actcgaccta
      181 tggctacaag gtggacgtcc aggacgccgt gcagttcgac aacagcggtg actacgtgca
      241 cagcgcgccg tggtcggtgg ccgaccaggg caagcgcgat gtcagccacg gctgcatcaa
      301 catcagcccg tccaatgcgc agtggttctt cgacaacttc ggcagcggcg atcccgtcgt
      361 cgtgaagaac tccgtcggca cctacagcaa gaacgacggc tccaacgact ggcagctcta
      421 gttccagatc ctgactcgcc gttggggatc caggtaaagc tcgtcggtct cggtgagctc
      481 gaaggcgtcg tagaagacgt cgatattgcg gatgactccg ttgcagcgga actccggcgg
      541 cgagtgcggg tcgatggcga gtcgccgaat cgcctcggcg tcacgggatt tggtgcgcca
      601 cacctgagcc cagccgaaga acacccgctg caccccggtc aggtcgtcga tgacaggcgc
      661 ctccgcgccc ttcagggaca gctgataggc gagcagcgcg atggacagcc caccgagatc
      721 gccgatgttc tccccgacgg tgaacgcgcc gttgacgtgg tgggaggcgc cgaggctacg
      781 aggagtgaac acctcgtact gctcgatgag agcctttgtg cgggaaccga actcggcgcg
      841 gtcgttgtcc gtccaccagt cgatcaggtt gccgtcaccg tcgtacttgg cgccctggtc
      901 gtcaaagccg tggccgatct cgtggccgat caccgcgccg ataccaccgt agttcgcggc
      961 gtcgtcggct tcggcgtcga agaacggagg ctgcagaatc gctgccggaa agacgatctc
     1021 gttcattccg gggttgtagt aggcgttgac ggtctgcggt gtcatgaacc actcgtcgcg
     1081 gtcgacgggc ccgccaagct tggccaggtc gcggtcatac tccagtgcat agccgcgttg
     1141 gtagttgccg tacaggtcgt cgcgggcgat gacgagcgac gagtagtcgc gccacttcgc
     1201 gggatagccg atcttcggcg tgaacttgtc tagcttctcc agcgcccgct gccgggtctc
     1261 cggcgtcatc cactccaggt cggtgatgct gacccggtag gcctcccgca gattggccac
     1321 caactcgtcc atccgggcct tggcgtccgg cgggaagtgc cgctcgacgt agagccggcc
     1381 gacggcgtcg cccatcaggc tctcgaccag gccgacgccg cgcttccatc ggtcgcgcaa
     1441 ctgctcggtg cccgacaacg tgcggccgta aaacgcgaag ttctcgtcga cgatgtcgtc
     1501 gctcagcagc gacgcccgcc cgctgatcac gcgccagcgc agccagcgcc gccagtcctc
     1561 gatgtcctcg ctgtcccaca gcgcggcgaa cgtgatcaac gcgccgggtt ggtggacgac
     1621 aacctcgagg ggcccactcg cctgaccaga gaggtcagcc cccagcgcat gtagccacac
     1681 cggccagtca aaaccggttg cctcggagct taattcagcg aaggtgcgca ggttgtaggt
     1741 caggtcggcg tcgcggcgtt tgaccacgtc ccagtgtgcg gccgcaattt tggtctccag
     1801 cgcgacgatg tgtgcggcgg tctcgtccca gtcgccctcg tgctcgccgt agaccagcgc
     1861 gaacgtccgc gcgatgtgct tcgggtactc ggcgaggatc gccgcgtgct gcgggtcgcg
     1921 gtaatacgac tcatcgggta gccccaggcc cgactggctc aggtgcagca ggtagcgggt
     1981 ggaatttttc gaatcggtgt cga
//
LOCUS       DATCBJ010000207         2303 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_448905, whole genome shotgun sequence.
ACCESSION   DATCBJ010000207 DATCBJ010000000
VERSION     DATCBJ010000207.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2303)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2303)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2303
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_448905"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..412)
                     /locus_tag="VII50_04185"
     CDS             complement(<1..412)
                     /locus_tag="VII50_04185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5050076.1"
                     /translation="MGLATAMFYGIMMIWACVTPDFRAPDEPQQVSTTLRLAYVHSYP
                     APGDARIYLPVKASYADVGFGGIGLNTIIGLSPLHRPTSKQLTSQTMRELGAAEPSAV
                     TPYDYDQMTQHPPGYFAVMAVAARLFNVIDKTPHT"
     gene            complement(469..1341)
                     /gene="rfbD"
                     /locus_tag="VII50_04190"
     CDS             complement(469..1341)
                     /gene="rfbD"
                     /locus_tag="VII50_04190"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008831 - dTDP-4-dehydrorhamnose reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HEY5050077.1"
                     /translation="MTERWLVVGAGGQLGTDLMRVLDGQYVIGLTRHELDITDVDAVA
                     RAVAAVVPSVIVNAAAYTAVDVAETDEATAFTANVTAPAALAAAAAIAGAKLVHVSTD
                     YVFAGDATAPYDEAAATGPKSAYGRTKLAGEQAVFASLPDAYVVRTAWVYGASGANFV
                     KTMARLERERETVSVVDDQRGSPTWSHDLARGLVALGSSGAGGGIYHCTNTGETTWFG
                     FTRAIFEELGADPERVLPTTTAAFPRPAPRPAYSVLGNRRWIEAGLEPLPHWRAALHD
                     AFASAPDELRGAAK"
     gene            complement(1338..>2303)
                     /gene="rfbB"
                     /locus_tag="VII50_04195"
     CDS             complement(1338..>2303)
                     /gene="rfbB"
                     /locus_tag="VII50_04195"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009072808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HEY5050078.1"
                     /translation="AAGFIGSHYVRSMLSDGYPGFEDAEVTVFDKLTYAGNLENLLPV
                     ADSPRYRFVQGDICDPAQVASAVAGHDIVVNFAAESHVDRSIYGAAEFVVTNVLGAQT
                     LFDACVRSGIGRVVHVSTDEVYGTIPEGSWTESHILEPNSPYSASKAGADLLARAYAG
                     TYGLNISVTRCTNNYGHYQFPEKVIPLFVTNLIDGLSVPLYGTGDNIRDWLHVDDHCR
                     GIQLVVEKGAAGEFYNIGGGIELSNKELTQRLLDACGATWDSVVYVEDRKNHDLRYSL
                     DDAKLRAMGYAPLTSFDEGLTKTVQWYRDNQTWWRAIKAKAALAT"
BASE COUNT          410 a          769 c          736 g          388 t
ORIGIN      
        1 cggtgtgcgg cgtcttgtcg atgacgttga acaggcgcgc cgcgaccgcc atcacagcga
       61 agtatcccgg cgggtgctgc gtcatctggt cgtagtcgta cggcgtcaca gcgctgggct
      121 cggctgcacc aagctcgcgc atcgtctgcg acgtcagttg cttggacgtc ggccggtgca
      181 gcggcgacaa tccgatgatc gtgttgagac cgatcccgcc gaacccgacg tcggcgtacg
      241 acgccttcac cggcaggtaa atgcgcgcat caccgggcgc cggatagctg tgtacgtagg
      301 cgagccgcag cgtcgtcgac acctgttgcg gctcgtccgg tgcgcggaag tctggagtca
      361 cgcacgccca gatcatcatg atcccgtaga acatcgcggt cgcgagcccc acgaccagtc
      421 caatgctcga gatccgagag cgggcgacgg ctcggtgaga agcctccgtc acttcgccgc
      481 gcctcgcagc tcatcgggtg cgctagcgaa cgcgtcgtgc aaagcggcgc gccagtgcgg
      541 caacggctcg aggcccgctt cgatccaccg acggtttccg agcaccgaat acgcggggcg
      601 cggcgccggg cgcgggaatg ctgcggtcgt ggtcggcaac acccgctccg ggtcggcacc
      661 gagctcctcg aagatggccc tggtgaagcc gaaccacgtg gtctcaccgg tatttgtgca
      721 gtgatagatg ccgccgccgg cacctgacga gccgagcgcc accaggccgc gagccaggtc
      781 gtgcgaccag gtcggagacc cgcgctggtc gtcgacgaca gacacggtct cgcgttcgcg
      841 ctcgagcctc gccatcgtct tcacgaagtt cgcgccgctc gcgccgtaga cccacgcggt
      901 gcgcacgacg taagcgtcgg ggagcgaagc gaaaacggct tgctcccccg ccagcttggt
      961 ccgcccgtac gcggacttcg gacccgtcgc cgcagcctcg tcgtacggcg ccgtagcgtc
     1021 gccggcaaac acatagtcgg tcgagacgtg cacgagcttg gcgcctgcga tcgcggcggc
     1081 cgcggccagc gctgcgggcg ccgtcacatt cgcggtgaag gcggttgctt cgtcggtctc
     1141 ggccacatcg acagcggtgt aggccgcggc attcacaatc actgacggga cgacggcagc
     1201 aacggcccgt gcgacggcgt caacatcggt gatgtcgagc tcgtgtcgcg tcaagccaat
     1261 gacatattga ccgtccagga cgcgcatcag gtcagtgccg agctgccctc ccgcgccaac
     1321 gaccagccac cgctcggtca cgtcgcaagt gccgccttcg ccttgatcgc acgccaccac
     1381 gtttggttgt cgcggtacca ctgcacagtc ttcgtgaggc cctcgtcgaa ggacgtaagc
     1441 ggtgcgtatc ccatggcgcg caacttcgcg tcgtccaatg aatagcgaag atcgtggttc
     1501 ttgcggtcct cgacgtacac caccgaatcc cacgtcgctc cgcacgcgtc aagcagccgc
     1561 tgcgtcagtt ccttattcga aagctcaatg ccgccgccga tgttgtagaa ctccccggcc
     1621 gcacccttct cgacaacgag ctggataccg cggcagtggt catcgacatg cagccagtca
     1681 cggatgttgt cgccagtgcc gtagagcggc accgacaggc catcgatgag attcgtgacg
     1741 aacagcggga tgaccttctc cgggaactga tagtgcccgt agttgttcgt gcaccgcgtc
     1801 accgagatgt tcagcccgta ggtgccggca taggcccgcg cgagcagatc tgcccccgct
     1861 ttgcttgcgg aatagggaga gttcggctcg aggatgtgag attccgtcca cgagccttcg
     1921 ggaatcgtgc cgtacacctc gtctgtcgag acgtgcacga cgcgcccgat gcccgaccgc
     1981 acacacgcgt cgaacaaggt ctgggcaccg agcacgttcg tcacgacgaa ctcggcagcg
     2041 ccgtagatcg aacggtcgac atgcgattcg gccgcgaagt tgaccacgat gtcgtgacca
     2101 gcgaccgcgg atgctacctg cgccgggtcg cagatatcgc cctgcacgaa ccgataacgc
     2161 gggctgtccg cgactggcag gaggttctcg agattgccgg cgtaggtgag cttgtcgaag
     2221 accgtgacct cggcgtcctc gaaccccggg tacccgtccg agagcatgct gcgcacatag
     2281 tgcgaaccga taaacccggc agc
//
LOCUS       DATCBJ010000208         7765 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_79788, whole genome shotgun sequence.
ACCESSION   DATCBJ010000208 DATCBJ010000000
VERSION     DATCBJ010000208.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 7765)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7765)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7765
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_79788"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(114..602)
                     /locus_tag="VII50_04200"
     CDS             complement(114..602)
                     /locus_tag="VII50_04200"
                     /inference="COORDINATES: protein motif:HMM:NF013561.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix protein, CopG family"
                     /protein_id="HEY5050079.1"
                     /translation="MANAKDYQAAADWAELEMVLKPGSTTALRGADAAKRGREVLAKA
                     LGGRPSIDPDAKPGQHARVRQVRVSAEVDARIEILAADRHVKASDIIREALAAYLASA
                     VSPGEKDASRTRRAKALAPAKAARARTPAKAVPAAAAQRVSVSAKTARTRNGVPAKRP
                     AK"
     gene            complement(602..817)
                     /locus_tag="VII50_04205"
     CDS             complement(602..817)
                     /locus_tag="VII50_04205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5050080.1"
                     /translation="MHAIEHAVYVEVEFDEPRLGGRVRPTLFIGPPRAPGGPLLEVMV
                     EITGSRGLHIFHVMQARKKHLDRMGEN"
     gene            complement(963..1298)
                     /locus_tag="VII50_04210"
     CDS             complement(963..1298)
                     /locus_tag="VII50_04210"
                     /inference="COORDINATES: protein motif:HMM:NF016876.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HEY5050081.1"
                     /translation="MTAKYNQTFDPAVLTVDLPALAAADPGLVDAALAAVDDLAARRQ
                     IGKELGARNISGDLTGLRRLRFDLPGVRTKRYRVVYKLEAHTIEVLAIGERRDHVIYR
                     AAVGRLPAD"
     gene            complement(1295..1585)
                     /locus_tag="VII50_04215"
     CDS             complement(1295..1585)
                     /locus_tag="VII50_04215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5050082.1"
                     /translation="MTIGEVLPTNEARARLNAIAATFDADGSHAEPVVFGSHRRAQGV
                     IVSWELWQRIAPAVEDVLDAELATQRATASAGRRLSRADIDAALGRSPSTDA"
     gene            complement(1734..2483)
                     /locus_tag="VII50_04220"
     CDS             complement(1734..2483)
                     /locus_tag="VII50_04220"
                     /inference="COORDINATES: protein motif:HMM:NF017102.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HEY5050083.1"
                     /translation="MNTTTATARRFRTLGSCVASCGVAGAILLAASPAQAYVSGYQIS
                     GSDGANVHSAASVDAKVVAFLDHGRSIQINCQTSGSTYNGSNIWDKLQSGGYVSDDLV
                     NTPVFDKYTPGIPRCPQPPAPPVKTGRIASGNEGTAGQCTWYAISRFHSFSGLYPYLI
                     SPGNSGDARYWATNAALDGWTVSATPRVNSIVVFPPGVNGAESDGHVAWVTAVGGGKI
                     TFAEMNGTAGPFEVDTRTVPPASTVRYILAP"
     gene            complement(2733..2939)
                     /locus_tag="VII50_04225"
     CDS             complement(2733..2939)
                     /locus_tag="VII50_04225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5050084.1"
                     /translation="MPQLLDFIDDWLDTDPAHLDPSLARFVGPPYNIEQLRTDLQRFT
                     FLLGHDDGEQLFEHATDDKCGQIT"
     gene            3071..3481
                     /locus_tag="VII50_04230"
     CDS             3071..3481
                     /locus_tag="VII50_04230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5050085.1"
                     /translation="MTSVTIKGRNQGANKVTWYGIAPSSNHSETTTGWWWARSVSIIT
                     KYDEWGVAACRTQIVNTVLIPADYSSNVFPVTIPLDAECGRLNATLPPQVRDPAAHRE
                     GLPGFVVGASASVAEVGRSARGRWFGQLDLYRSS"
     gene            3438..6380
                     /locus_tag="VII50_04235"
     CDS             3438..6380
                     /locus_tag="VII50_04235"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEY5050086.1"
                     /translation="MVDGLVSSIFIGRRDALAAIGAAQRDALAGSSVAVVVAGEAGVG
                     KTRFLTESQQLAVATGMAVAGGRCVELSQEGVSYAPLVGALRSLTRALDAEQLETVLG
                     HARRELARLLPELDPSVGAEPSVPGQAARLFELVLGVLERAAAHQPLMLVIEDLHWAD
                     RATLDLVSFLVAELRGAPLLLVLTYRSDEMHRSHPLRAVLSGWSRARQVRPIELLPLT
                     RAEVASLLKAIMGDEPGADLVDRVQRRSEGNPFLAEEFIGVMRDGSDPDRLPDSIREL
                     LLARLTATTPEVQALLRAVSAAAGRPTGDELLAVVTGQDRATRQESLRAAVEHQLLVV
                     DDSGGFVFRHALTREAVYDDMLPGERSQLHAAFGEALDGDERLAGDGAPVDALLAYHW
                     DAARDLARAFAASVRAAGSSSMSFAPDQALAHLERAVGLWPRVPDAAQLSDTTLVDLL
                     VTASDLAIVAGAPAKALTLADQALAALPPDQPARRAVLLEQKAAALRDLSRVDEGAQV
                     LAEALNIVPAGCHRERGIVLSAIGGNLVASDTAAALPLLEEAIKELAQGPSDRRLADA
                     FAMLGAVHIRLGDLEEGMTSLATAIRIAKETGTVDTLVLAQLASSDVFGMLGRHNDAV
                     AGAQEALEAARRRGVLHSARAGLTVCNLVEALIRLGRWPEADALLAEHGGRESAPYAG
                     AALRVLAAQLAVARGDGRSAASNVQRALDLDAGVYDIDLDAAFVNSEVARLAGEVEHA
                     QRIVIDALQAVQPEWEERYVWPLVWQGLRATADCAGSRNSQQGDKLEMLAAITSRLPG
                     RGPLAQAYRALADAERARADDAPAAQSWQDVLVGWRELAEPLMTGYCLLRVAEARRTV
                     GQRDLAADALHEGLAIAKRLGAQPLVDDFTGLARRARITLDDEPDAAPESDAVTPGDR
                     FELTDRERQVLTLIAEGKTNKEIAVALYISPKTAGIHVSHILDKLGVKGRVEAATLAV
                     QHGLVSRG"
     gene            complement(6860..6997)
                     /locus_tag="VII50_04240"
     CDS             complement(6860..6997)
                     /locus_tag="VII50_04240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5050087.1"
                     /translation="MGVVWRLDCSPGQPSSVVASKVERARRVTLELLERGDIEIWLMN
                     S"
     gene            7103..7522
                     /locus_tag="VII50_04245"
     CDS             7103..7522
                     /locus_tag="VII50_04245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5050088.1"
                     /translation="MADTEADDVADEELLGFCVVANVAAETAHGIGGVDIRSGVQHFS
                     AGSKVWVLPPQWGDGGDQLIVVGHHRGTRGRGYSRIVMPRRHLTNFRVQGIYQPALLR
                     ALVALQDRSPEVPRLWAGRDDAERFAAMWREDRPLQR"
     gene            complement(7533..>7765)
                     /locus_tag="VII50_04250"
     CDS             complement(7533..>7765)
                     /locus_tag="VII50_04250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5050089.1"
                     /translation="AGPVVNLSACAVEQWTSRSLAAADSGQTSGLITVSFTDSYDINV
                     NAYNAADVRLAFVTVSAGRPLTLTSADQQICG"
BASE COUNT         1273 a         2455 c         2633 g         1404 t
ORIGIN      
        1 tgaatagctt tggctggttt tgactgtcgt gttgcccatc tgttgcccaa cagtgatcaa
       61 cttctcccca ccgatctccg ccaatgactg tcgcaactac acagtcttgg cggtcacttg
      121 gcgggccttt tggctgggac accgttccgc gttcgggccg tctttgcact caccgatacg
      181 cgctgcgcag ccgctgccgg gacagccttc gccggcgtcc gggcacgtgc agctttggca
      241 ggagccaatg ctttggcacg ccgcgttcgt gacgcgtcct tctcgcccgg actcaccgcg
      301 ctggcaaggt aggccgccaa cgcctcccgg atgatgtcac tcgccttgac atggcgatct
      361 gcagccagga tctcaatgcg ggcatcaacc tcggcagaga cccggacctg ccggacccgc
      421 gcgtgctgac ccggcttcgc gtcagggtcg atcgaaggcc gaccacccaa cgccttcgca
      481 agaacctcac gtccgcgctt ggccgcatca gctccgcgca acgcagtcgt cgagccgggc
      541 ttcaaaacca tctcgagctc ggcccagtcg gccgcagcct ggtagtcctt cgcgttcgcc
      601 atcagttctc tcccatccgg tcaaggtgct tcttacgcgc ctgcatcacg tggaatatgt
      661 gcagacctct ggagccggtg atctccacca tcacctccaa cagcggcccg ccaggtgctc
      721 gcggcggccc gatgaacaac gtcggacgca cccgcccgcc cagccgcggc tcatcgaact
      781 cgacctccac gtagacggcg tgctcgatcg cgtgcaaagc atcctcatgc gggacgccat
      841 gcttgtccgc gctggctgcc cagcttgatc gccacccatg taatgtacta caaaacattt
      901 ctcggttgtg cagtacacga cctggcccgc tggatgcggg agatgaagcg ccgtggcggt
      961 ggtcagtcgg cggggaggcg gccgacggcg gcgcggtaga tgacgtggtc gcggcgttcg
     1021 ccgatggcga ggacttcgat ggtgtgcgct tcgagcttgt agacgacgcg gtatcgcttt
     1081 gtgcggacgc cgggcaggtc gaagcgaaga cgacgaagcc cggtcaggtc tccactaatg
     1141 ttgcgtgcgc cgagttcttt gccgatttgc cgcctggcgg cgaggtcgtc gacggcggca
     1201 agcgcagcat cgacgagtcc tgggtccgct gcggccaggg cgggcaggtc gacagtgagg
     1261 actgcggggt cgaatgtttg gttgtacttg gcggtcacgc gtcggtcgac gggctacgac
     1321 caagcgccgc gtcgatgtct gcccgcgata ggcgacgacc cgcagacgcc gtggcgcgct
     1381 gcgttgccaa ctccgcatcg agcacgtcct cgacggcggg agctattcgc tgccatagtt
     1441 cccacgacac gatgacccct tgtgctctgc ggtggctgcc gaagacaaca ggttcggcgt
     1501 gtgagccgtc ggcgtcgaag gttgcggcga tcgcgttgag gcgggcgcgc gcctcgttcg
     1561 tcgggaggac ctcaccgatg gtcatgagag aaagggtacc tcattcggta ccgatccgtc
     1621 cttgtgtcgc cctctatgag cgcgtactcg ccgatgacgc cgccgaatga tgtcgcgaca
     1681 tgcgaccgtc cgaacccggt atggccaggt tcggacggtc gcggttgacg cgatcagggt
     1741 gccaagatgt agcgcacggt cgaggctggc gggacggttc gggtgtcgac ctcgaagggg
     1801 cctgccgtac cgttcatctc ggcgaaggtg atcttgccac cgcccacagc agtgacccac
     1861 gcgacgtgac cgtctgactc cgcgccgttg acgcctggcg ggaagacgac aatcgagttc
     1921 acccggggcg tagcgctgac agtccacccg tcgagggccg cgttagtcgc ccaatagcgc
     1981 gcgtcaccgc tgttccccgg gctgatcagg tacggataca agccgctgaa gctgtggaag
     2041 cgggagatcg cataccaggt gcactgaccg gcggtgcctt cgttgccgga cgcgatgcgg
     2101 ccggtcttga ctggcggggc cggcggctgc gggcagcgcg ggatgccagg cgtgtacttg
     2161 tcgaataccg gcgtgttcac caggtcgtcg ctgacgtacc cgccggactg caacttgtcc
     2221 catatgttgc tcccgttgta cgtggacccg gacgtctggc agttgatctg gatcgaccgg
     2281 ccatggtcca ggaacgccac aaccttggcg tcaaccgaag cggccgaatg cacgttcgca
     2341 ccgtcggaac cgctgatctg atagcccgac acataggcct gagccgggga ggccgcgagc
     2401 aagattgcac ccgcgacgcc acacgatgca acgcaagacc cgagggttcg gaatcgccgg
     2461 gcggtggcgg tggtggtgtt catgactgtc tcctcgctgg gtgtcggggc cgtcccttgt
     2521 ggtcggcgcc gttctaagga gaggttggcc gagatcccag ccgcttccat cgggaacgca
     2581 gggtggatgt cgtcggtcgg cgccctatct ctgcggtggc aagcgatgcg acatcggctg
     2641 gtgtagggca accgccctat ccggccagtc atatgaccgc tcgtcaggca gggcacgtag
     2701 gttgcttgcc gcgaccccga acgtgggtgt cgtcaagtga tctggccgca cttgtcgtcg
     2761 gtggcgtgct cgaagagctg ctcgccgtcg tcgtgtccga gcaggaatgt gaagcgctgc
     2821 aggtcggtcc gcagctgctc gatgttgtag ggcggcccga cgaacctcgc gagtgacggg
     2881 tcgaggtggg cggggtcggt gtcgagccag tcgtcgatga agtccagaag ctggggcaaa
     2941 gctggcttcg tgcgtcgctc ttgccgcggg attgtccgtt gcgggtttcg tggtcccgac
     3001 gagttcggcc gtagctggga cgaacaggca gcagattcgg gtcacctttg ttggccccgg
     3061 cgcctcgagc gtgacctcgg tcacgatcaa gggacgcaat cagggcgcga ataaagtgac
     3121 gtggtacggg atcgcgccgt catcgaatca ttcggagacc acgacgggct ggtggtgggc
     3181 acgctcggtg tcaatcatca cgaagtacga cgagtggggc gtggccgcct gccgcacgca
     3241 aatcgtcaac acagtcctga ttcccgctga ctactccagc aacgtcttcc ccgtcacgat
     3301 ccccctcgac gctgaatgcg gccgactgaa cgcgacactc ccaccgcagg tacgagaccc
     3361 ggcagcccac cgtgaagggc tgcccggttt cgtcgttggt gctagtgcat cggtcgctga
     3421 ggtgggacgc tctgcgcgtg gtagatggtt tggtcagctc gatctttatc ggtcgtcgtg
     3481 atgcgttggc ggcgatcggt gcggcgcagc gtgacgcgtt ggcgggttcg tcggtcgctg
     3541 tcgtggtggc tggtgaggcg ggggtgggca agacccggtt tctcaccgag tctcagcagc
     3601 tcgccgttgc cacgggtatg gcggtggcgg gcggccggtg tgtcgagttg agtcaggagg
     3661 gtgtcagcta cgccccgctg gtgggtgcgc tgcggtcgtt gacgcgggcg cttgacgctg
     3721 agcagctcga gactgtgctt gggcacgccc ggcgggagtt ggcgcggctg cttcccgagc
     3781 tcgatccgtc ggtgggtgca gagccgtcgg ttccgggtca ggcggctcgg ttgttcgagt
     3841 tggtgctcgg ggtgctggag cgcgcggcgg cccatcagcc gctgatgctg gtcatcgagg
     3901 acctgcattg ggcggaccgc gcgacactgg acctggtgtc gttcctggtt gccgagttac
     3961 gcggtgcgcc gttgctgctg gtgttgacct accgctccga tgagatgcac cggtcgcacc
     4021 cgctgcgagc ggtgttgtcc gggtggagcc gcgcccgaca ggtacgcccg atcgagctgc
     4081 tacctctgac gagggcggag gttgcgtcgc tgttgaaagc gatcatgggt gacgagccag
     4141 gcgctgatct agtcgaccgg gtgcagcggc gatcggaagg caacccgttc ctcgccgaag
     4201 agttcatcgg agtgatgcgc gacggctccg acccggaccg gctgcccgac tcgatccgtg
     4261 aactgctcct cgcgcggctg accgcgacca cgcccgaagt tcaggcgctg ctgcgagcag
     4321 tgtcggccgc ggctggccgc ccgaccggcg acgaacttct cgcagtggtg acgggtcagg
     4381 atcgggcgac acggcaagaa tcgttgcgtg cggcagtcga gcatcaactg ctggttgtcg
     4441 atgacagcgg cggattcgtg ttccggcacg cgctgacccg cgaagcggtc tacgacgaca
     4501 tgttgcccgg tgaacgctcg caactgcatg cggcgttcgg cgaggctctc gacggtgatg
     4561 agcgcctcgc cggcgacggc gcaccggtcg atgcgttgct cgcctaccac tgggacgccg
     4621 cgcgggatct ggcgcgagcg ttcgcggcgt cggtgcgggc agccggatca tcgtcgatgt
     4681 cgtttgctcc tgaccaggca cttgcgcacc tagagcgcgc ggtcggactg tggccccggg
     4741 tgcccgatgc cgcccagctg agcgacacca ctttggtcga tctgctggtg acagcgagcg
     4801 accttgcgat cgtcgccggc gcacccgcga aggcactgac cctcgccgat caggcgctgg
     4861 ctgctctacc cccagatcag ccggcccgac gagcggtgct gctcgaacaa aaagcggccg
     4921 cgcttcgcga tctcagtcgc gtcgatgaag gggcgcaggt tctcgccgag gccctgaaca
     4981 tcgttccagc cggctgtcat cgagaacgcg gaatcgtatt aagtgcaatc ggtggcaacc
     5041 tcgtcgcctc ggacaccgcc gcagcgctgc cgctgctcga agaggccatc aaggagttag
     5101 cgcaaggacc ctcggatcgg cgcctcgccg atgcctttgc catgttgggt gccgtgcaca
     5161 ttcggctcgg cgatctcgag gaagggatga cttccctcgc caccgcgatc cggatcgcca
     5221 aagagacggg caccgtcgac acccttgtgc tggctcagct ggccagctcc gatgtgttcg
     5281 gcatgctcgg tcgccataac gacgcggtgg ccggcgcgca ggaagcgttg gaggctgcgc
     5341 gacgccgcgg cgtcctacac tcggcgagag cgggcttgac cgtgtgcaat ctcgtcgaag
     5401 cgttgatccg gctgggacgc tggcctgagg cagatgcgct gctggccgag catggtgggc
     5461 gagaaagcgc cccgtatgcg ggcgccgcat tgcgagtgct cgccgctcag ctcgctgttg
     5521 ctcgcggcga cggtcggtcc gcagccagca acgtccagcg cgccctggat cttgacgccg
     5581 gcgtctacga cattgacctc gacgctgcat tcgtcaacag cgaagtagcg cgcctggccg
     5641 gggaggttga acacgcacag cgcatcgtca tcgatgcgct gcaagccgtt cagccggagt
     5701 gggaagaacg ctacgtatgg ccgttggtgt ggcagggact gcgtgccacc gccgactgcg
     5761 ccggttcacg aaactcccag cagggcgaca agctcgagat gttggccgcg atcaccagca
     5821 gactccccgg acgtggtccg cttgcgcagg cctaccgcgc gttggctgat gccgagcgag
     5881 cacgagccga cgatgctccg gctgcccagt cgtggcagga cgtgttggtc ggatggcgtg
     5941 agcttgctga gccgctgatg acggggtact gcctcttgcg tgtcgccgag gcgcgacgga
     6001 ccgtagggca gcgcgatctc gccgcggatg cattgcacga aggtctcgcc atcgcgaagc
     6061 gtctcggcgc gcaacctttg gtcgacgact tcaccgggct ggctcgacgc gcccgaatca
     6121 ccctggacga cgagcccgac gccgcgcccg agtccgacgc cgtcacaccg ggcgaccggt
     6181 tcgagctaac tgatcgcgag cggcaggtgc tgacgctcat cgccgaaggc aagaccaaca
     6241 aagagatcgc cgtggcgctc tacatcagcc cgaagaccgc cggcatccac gtatcccaca
     6301 tcctggacaa gctcggtgtt aaaggcagag tcgaggccgc gacgctcgcg gttcagcacg
     6361 gcttggtgag ccgtggatga cgcgccggcc cgaagtcacc tatcgcgcgc gcttctggtt
     6421 cgatcccgga tcgggagtct gcctgtgggg agcccgatac ggccaccgtc gaagcgttca
     6481 actatccgat cgacacccgc cgactgccac tcaccgataa cctccgctac gcccttgacg
     6541 ccctgatcgc gatcttcaac acctcgatcg actgggccta gccgcccgac cccagtccct
     6601 ggtcagccga gcaatggcag cgctggcgcc agagcggaga tgagttgctg gttcgactcc
     6661 gcgccgaact cggcgccggc tgggagatca acgacgagcg caacgcacgc cacggcaacc
     6721 cacccggtgg ggtctaaggc gtctcgcgca accgaatctc atacaaccgg ctggctcggt
     6781 ccggcgcgaa ccacagcagg tcgtccgcct gctcgatgag gaaatctgca agagcaactg
     6841 gtgtgtaagc agatggtggt caagaattca tcaaccagat ctcgatgtca ccacgttcga
     6901 gcagttcaag ggtcacccgc cgcgcccgct cgaccttcga ggcgaccact gacgagggct
     6961 gcccgggaga gcagtccagc cgccacacga ctcccacagt gcttgcacgc cctcctcggt
     7021 caggctatcc aacaggtcag cccgcatcgc atccgccgac gcatcagacg acatatcccg
     7081 cgtcttcctc gatcattggt gagtggcaga caccgaggct gacgacgttg cggatgagga
     7141 gctgctgggg ttctgcgtcg tggcgaacgt cgctgccgag acggcgcacg ggatcggagg
     7201 cgtggacatc cgaagcggtg tccagcattt ctccgccgga tccaaagtct gggtgctccc
     7261 tccgcagtgg ggtgacggcg gagaccagtt gatcgtggtg ggtcatcatc gaggaacccg
     7321 aggtcgcgga tactccagga tcgtgatgcc gcgacggcac ctgacgaact ttcgggtcca
     7381 agggatctac cagcccgcgt tgcttcgcgc actggtggca ctacaggatc gcagtcctga
     7441 ggtgccgcga ctgtgggccg ggcgagatga cgccgagcgg ttcgcggcga tgtggcgtga
     7501 ggatcgtccg ctccaacgat gagccgtggg tctcacccac agatctgttg atcggcggag
     7561 gtcagtgtca atggtcggcc cgcggacacg gtcacgaatg cgaggcggac gtcggcggcg
     7621 ttgtaggcgt taacgttaat gtcgtagctg tcagtgaagc tcaccgtgat gagtccgctg
     7681 gtctggcccg agtccgcggc cgcgagggat cggctcgtcc actgctcgac cgcgcaggcg
     7741 ctgagattga cgactggccc ggcaa
//
LOCUS       DATCBJ010000209         2445 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_190905, whole genome shotgun sequence.
ACCESSION   DATCBJ010000209 DATCBJ010000000
VERSION     DATCBJ010000209.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2445)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2445)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2445
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_190905"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            172..>2445
                     /locus_tag="VII50_04255"
     CDS             172..>2445
                     /locus_tag="VII50_04255"
                     /inference="COORDINATES: protein motif:HMM:NF013727.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015616 - DNA translocase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HEY5050090.1"
                     /translation="MIFAHVVGATFRALGARARDLDPAHRRDGAGLAAIGAALVIAGG
                     VWWRLPGPIGTVIVAVVRGAVGSGAVVVPVLLVALAWRTLRHPDRPSASGRIVIGWGA
                     VTLGVLGLLHVAHGSPDPSLGASAVRHAGGTIGFMVAAPLESGLTVWLTVPLLGLLGA
                     FGVLVVTATPVNGIPQRLAELRDFVLRRKPPTESDLLIDLNAEPPAALRRGSRRRRVG
                     VFSDAPAEGDRPYDTPLLADGDAASVPVAADAAAEGGTDGVHLGGAGAGAARKPGQPV
                     GLPARAEQLLLSGDITYHLPPPTMLREGSHARARTKANDFVVDALTTVLEQFEIDATV
                     TGFTRGPTVTRYEVELGPAVKVERITALSKNIAYSVASADVRILSPIPGKSAVGIEIP
                     NADRDVVSLGDVLRSPVATGDHHPMVVALGKDVEGRFICANLAKMPHILVAGATGSGK
                     STCINTIVTSILTRATPDEVRMVLVDPKRVELTSYEGIPHLVTPIITSPKKAAEALQW
                     VVREMDMRYDDLAASGYRHIDDFNRAVNTGTLVPPPGSERVYRPYPYLLVIVDELADL
                     MMVAPRDVEDSIVRITQLARAAGIHLVLATQRPSVDVVTGLIKANVPSRLSFATSSLA
                     DSRVILDQAGAEKLVGLGDALFLPMGASKAIRLQGAFVSETEIEAVVAHCKQQLQPTY
                     REDVTLAAAATKREVDDDIGDDLDLLCQAIELVVTTQFGSTSMLQRKLRVGFAKAGRL
                     MDLMESRGMVGPSEGSKA"
BASE COUNT          377 a          824 c          831 g          413 t
ORIGIN      
        1 ccacactcga ccccggctgc gcgcccacgc accggcgggc gaccacccgc ccggcggccg
       61 gccccgccgc gccgtcggcc ccgcgcgcgc cggaaccgcg gcggcgggcg catcgccgca
      121 ctcggcggcg ggctgttccg cgcgaccagt cgtctcttgc gaggcacctg gatgatcttc
      181 gcccacgtcg tcggcgccac gttccgggcg ctcggtgcgc gggcacgcga cctggatcct
      241 gcgcaccgcc gcgacggcgc cggccttgcg gccatcggcg ccgcgctcgt catcgcaggt
      301 ggggtgtggt ggcggctgcc gggtccgatt ggcaccgtga tcgtcgccgt cgtacgcggg
      361 gccgtcggct cgggcgcagt agtcgtgccg gtgctgcttg tagcgctcgc gtggcgaacc
      421 cttcgccatc ccgaccgtcc atcggccagt ggtcgcatcg tgatcggatg gggggcggtc
      481 accctcggcg tcctcggctt gctacatgtt gcgcacggca gccccgaccc gagccttggc
      541 gcgtcggccg tccgccacgc cggcggaacc atcggtttca tggtggccgc gccgctcgag
      601 tccggtctca ccgtgtggtt gacggttcca ctcctgggcc tgctcggtgc attcggcgtg
      661 ctcgtcgtga ccgcgacccc ggtgaatggg attccgcaac gtcttgcgga gttgcgtgac
      721 ttcgtgcttc ggcgcaagcc cccaactgag tccgacctgc tgatcgacct caacgcggaa
      781 ccgccagcgg cgctgcgccg cggcagccgt cggcgccggg tcggggtgtt cagcgacgca
      841 ccggccgaag gcgaccgccc gtacgacacc ccgctgctcg ccgacggcga cgccgcatcg
      901 gtgcctgtgg cggcagatgc agcggccgag ggcgggactg acggggtcca tcttggtggt
      961 gcgggcgccg gtgccgcgcg caagcccggt caacctgttg gattgccggc gcgggccgag
     1021 cagttactgc tgtcgggcga catcacttac cacctgccgc cgcccacgat gctgcgcgag
     1081 ggatcacacg cgcgcgcacg gacgaaggcg aacgatttcg tcgtcgacgc attgacgacg
     1141 gtgctcgagc aattcgagat cgacgcgaca gtgaccgggt tcactcgcgg cccgacggtg
     1201 acccgctacg aagtcgaact cggcccagcg gtgaaggtcg aacgcatcac ggcgctatcg
     1261 aagaacatcg cctactcggt ggccagtgcc gacgtccgga tcctgtcgcc gatccctggc
     1321 aagtcggcgg tcggcatcga gatccccaac gccgaccgcg acgtcgtgag cctcggtgac
     1381 gtgcttcggt cgccggtcgc gaccggcgat caccacccga tggtggttgc gttgggcaag
     1441 gacgtcgagg gccgcttcat ctgcgcgaac ctggcgaaga tgccgcacat cttggttgcc
     1501 ggcgcgactg gttccggcaa gtcgacctgc attaacacga tcgtcacgtc gattttgact
     1561 cgcgccacac ccgacgaggt gcgcatggtt ctcgtcgacc ctaagcgggt tgagttgacg
     1621 agctacgaag gcatcccgca cctcgtgacg ccgattatca ccagcccaaa gaaggccgcc
     1681 gaagcgttgc agtgggtcgt gcgcgagatg gacatgcgct acgacgatct cgcggcgtcg
     1741 ggttatcggc acatcgatga cttcaaccga gcggtcaaca ctggaacgct ggtcccgccg
     1801 cccggcagcg agcgggtcta tcggccgtac ccgtatctgc ttgtcatcgt cgacgagctc
     1861 gccgatctga tgatggtcgc gccgcgcgac gtcgaagact cgatcgtgcg gatcacccag
     1921 ctcgcacgcg ccgccggaat tcacttggtg ttggcgacgc aacgaccgag cgtcgacgtc
     1981 gtcacaggtt tgatcaaggc gaacgtgcca tcacgcttgt cgttcgccac ctccagcctc
     2041 gcggacagcc gggtgatcct cgaccaggca ggtgcagaga agctggtcgg tctcggcgat
     2101 gcactgttcc ttccgatggg agcgagcaaa gcgattcgcc tgcagggcgc cttcgtgtcg
     2161 gagaccgaga tcgaagctgt cgtggctcac tgcaagcagc agttgcagcc gacgtatcgc
     2221 gaagacgtga ccctggcggc cgccgctacc aagcgagagg tcgatgacga catcggcgac
     2281 gacctcgatc tgttgtgcca ggccatcgag ttggtggtca cgacgcagtt cggatccacg
     2341 tcgatgttgc agcgaaagtt gcgggtgggc ttcgccaaag caggacgtct gatggatctc
     2401 atggaaagtc gaggcatggt cggccccagt gagggctcga aagcg
//
LOCUS       DATCBJ010000210         6055 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_522908, whole genome shotgun sequence.
ACCESSION   DATCBJ010000210 DATCBJ010000000
VERSION     DATCBJ010000210.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 6055)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6055)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6055
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_522908"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..491)
                     /locus_tag="VII50_04260"
     CDS             complement(<1..491)
                     /locus_tag="VII50_04260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009072700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY5050091.1"
                     /translation="MSSAQLGPHYRESVLKEMGEGEFDLLVIGGGIVGAGAALDAVTR
                     GLTVALLEAHDWAAGTSSRSSKLIHGGLRYLEQRNFALVREALKERALLLNRIAPHLV
                     RPVPFLLPLQHRVWERFYIGSGVLLYDRMGGARAVPGHRHLSRQAALRLAPGMRSDAL
                     IGA"
     gene            complement(523..2040)
                     /gene="glpK"
                     /locus_tag="VII50_04265"
     CDS             complement(523..2040)
                     /gene="glpK"
                     /locus_tag="VII50_04265"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016292161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HEY5050092.1"
                     /translation="MADFVGAVDQGTTSTRFMIFDHGGNVKGLHQLEHQQILPQAGWV
                     EHNPAEIWERTRAVIQTALTKANLQSSDLAAVGITNQRETSVVWNKKTGRPYYNAIVW
                     QDTRTDRIASALEREGKGDVIRQKAGLPPATYFSGGKIQWILENVDGVRAAAEAGEAL
                     FGNTDSWLLWNLTGGVDGGVHVTDPTNASRTMLMDLETLQWDDELLGYFNIPRQMLPA
                     ILPSSHPGLYGTSRSNGPLGGEVALSGDLGDQQAATVGQVCFGVGEAKNTYGTGNFLL
                     YNTGHDVVRSKNGLLTTVCYKFGDEATVYALEGSIAVTGSAVQWLRDQLGIISGAAQS
                     ESLAAQVPDAGGIYFVPAFSGLFAPYWRSDARGAIVGLSRFNTNAHLARATLESICYQ
                     TRDVVEAMYQDSGVALDVLRVDGGITANKLCMQMQADILGVPVSKPVVAETTALGAAY
                     AAGLAVGFWKSTDELTKNWNEDLRWQPQWSADQRAKGYAGWKKAVERTLDWVDVG"
     gene            complement(2095..2943)
                     /locus_tag="VII50_04270"
     CDS             complement(2095..2943)
                     /locus_tag="VII50_04270"
                     /inference="COORDINATES: protein motif:HMM:NF012455.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MIP/aquaporin family protein"
                     /protein_id="HEY5050093.1"
                     /translation="MAERLRSRGLMGELASEFAGTFILIVLGVGVSAQVAAAGIGDHD
                     SIAWAWGLGVALGVYTAGKLSGGHINPAVTVAFAVFRGFPWRKVGPYALAQTAGAFVA
                     ALIVRWDYTEVIAKLDPKHTIKSQGIFSTLPGNGVLPVHESGAFRDQVIGTAILLFLI
                     VAITDTRNTAPAANLAPVVIGLLVVAIGMAWGTDAGYAINPARDFGPRLASFLTGYGT
                     AFRDQYGNLYFWVPIIGPLIGGIVGVGIYDALIGRFLPAEGPDEVGRLPEQSDGSDSQ
                     VSAETR"
     gene            3292..4077
                     /locus_tag="VII50_04275"
     CDS             3292..4077
                     /locus_tag="VII50_04275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5050094.1"
                     /translation="MLAGHHPADDWAALDSARYYASARLGVRGWHVGGVGRRLFFAVI
                     HPHLVPAYYRLLRKLPVETMVLSALPAGRLLYDRWSRQSWRKAASHACLLLPSDADSY
                     LRGRHRQAVRTNLHRADKIRLTCRPIPTDWGETKRSLHRGTFASLVKEIDRIEQRDAV
                     VKSWAVFDSGGQVLGRAVALVDERTAVLLLLHGPMDLSVAHQTRYLLHTAVVADLIRR
                     GVRNLVIESILGAPPGLKYFAARLGYRACRIQVVSTGRVPALS"
     gene            complement(4085..4714)
                     /locus_tag="VII50_04280"
     CDS             complement(4085..4714)
                     /locus_tag="VII50_04280"
                     /inference="COORDINATES: protein motif:HMM:NF044515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEY5050095.1"
                     /translation="MSAVTTPDAAERALRADAKRNREALLVCAGQAFAEHGTAASLED
                     VARRAGVGIGTLYRHFPTRDALVEAAYRHGVEQLCEAADQLLTEQPPDLALELWMRDF
                     VGYVATKRGLAATLKLSADDSHTELFAYVHERIRAAIKSLVDAASATGRIRSDVDGGD
                     LIRALGGICMVSDQVGWQDQARRLVSLLMDGLRYGALTPAQNPPGPSSR"
     gene            4815..>6055
                     /locus_tag="VII50_04285"
     CDS             4815..>6055
                     /locus_tag="VII50_04285"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY5050096.1"
                     /translation="MPVHSPPSRTADPIAVSPPSESKSGNRRSAALAIILLAQLMVVL
                     DATIVNIALPKIQTALSFSRTDLSWVLNAYSLTFGGLLLLGARSGDLFGRRRVFLAGI
                     ALFTVASLAGGFTTSSGLLLLARSIQGVGAAFASPSALALLMITFREGAERTRAIALY
                     TAVSIGGSAVGLVAGGVLVEWVSWRWVFFVNVPVGIVLLVLARRDLPETDRHTGRVDV
                     PGALTSTIGMAALVYGFVQAAASGWQAGSTIAAFTVGVVLLATFVLIETRVATPITPL
                     RLFADRDRVFSYLARLVLVGGMFGMFFFLTQFLQDVLGYSPLRSGLAFVPLTIALFAA
                     SQLAARVLLARVGPKRLMVGGLTTSTIGLLVLTQMSATSSYGLILAGLLLFGTGNGLA
                     FVPLTTASLAGVDPKDAGAASG"
BASE COUNT          953 a         1982 c         1978 g         1142 t
ORIGIN      
        1 atggctccga tcagcgcatc tgaccgcatg cccggcgcga gacgcaacgc cgcttgacgg
       61 ctcagatgcc gatggcccgg caccgctctc gcgccgccca ttcggtcgta aagcaagacg
      121 ccggagccga tgtagaaccg ctcccagact cggtgttgca gcggcagcaa gaacggcacc
      181 gggcgcacca gatggggcgc gatcctgtta aggaggagcg cgcgttcctt caatgcttcg
      241 cgcacgagtg cgaagttgcg ctgctcgaga tagcgcaacc cgccgtggat cagtttgctc
      301 gatcgcgacg acgtgccggc cgcccagtca tgcgcctcga gaagcgcgac ggtcaggccg
      361 cgcgtcacag cgtcaagcgc tgctcccgca ccgacgatgc caccgccgat gacaaggagg
      421 tcgaactcgc cctcacccat ttccttgagc acggattcgc gatagtgcgg tccgagttga
      481 gctgaactca cgtttttctc ctgcctaccc cgcgatgtcg ggtcagccga cgtccaccca
      541 gtcgagcgta cgttcgacag ccttcttcca gccggcgtag ccctttgcgc gctggtccgc
      601 cgaccactgg ggctgccaac gaaggtcctc attccagttc ttcgtcagtt cgtcggttga
      661 cttccagaac ccgaccgcga gacctgccgc gtatgccgcg ccgagtgccg tggtttccgc
      721 gacgacaggc tttgaaaccg ggacgccgag aatgtcggcc tgcatctgca tgcacagctt
      781 gtttgcggtg atgccgccgt caacgcggag tacgtcgagt gccacaccgg agtcctggta
      841 catggcctcg accacgtccc gggtctggta gcagatcgac tccagtgttg ccctcgcgag
      901 gtgggcgttg gtgttgaagc gggacagtcc gacgattgcg ccgcgagcat ccgagcgcca
      961 gtagggcgcg aagagccccg agaacgcagg cacgaagtag atgccaccgg catctgggac
     1021 ctgcgcggcc aatgactcgc tctgcgcagc accggaaatg atgccgagct ggtcgcggag
     1081 ccactgcacg gccgatccgg tgacagcgat cgaaccttcc agcgcataca ccgtcgcctc
     1141 gtcgccgaac ttgtagcaca cggtcgtgag caggccgttc ttcgaacgga ccacatcgtg
     1201 accggtgttg tagagcagga agttgcctgt tccgtaggtg ttttttgcct caccgacacc
     1261 gaagcacacc tggccgacgg tcgcggcctg ctggtcgccg aggtcgccgg aaagtgcgac
     1321 ctcgccgccg agcgggccgt tggatcgcga cgtgccgtac aaccctgggt gggaggacgg
     1381 caggatcgcc ggcagcatct gccgaggaat gttgaagtac ccgagcaact cgtcgtccca
     1441 ttgcaatgtc tcgaggtcca tcagcatggt ccggctcgcg ttagtcgggt ccgtcacatg
     1501 gacaccgcca tcgacaccgc cggtcaggtt ccacaacagc cagctatccg tgttgccgaa
     1561 cagcgcttcg cccgcttccg cggccgcgcg cacgccgtcg acattctcga ggatccattg
     1621 gatcttcccg cccgagaaat acgtcgcagg aggaaggccg gccttctgcc ggatgacgtc
     1681 gcccttgccc tcacgttcga gcgcggaggc gatccgatcc gtgcgggtgt cttgccagac
     1741 gatggcgttg tagtacggac gcccggtctt cttgttccac accacgcttg tttcgcgctg
     1801 gttcgtgatc ccgaccgccg cgaggtccga cgactgcagg ttcgccttgg tgagcgcggt
     1861 ctggatcacc gcccgggtcc gttcccagat ctccgcaggg ttgtgctcga cccagccagc
     1921 ctgcggcagg atctgctggt gctccaactg gtggagcccc ttcacgttgc caccgtggtc
     1981 gaagatcatg aagcgggtgc tggtcgtgcc ttgatcgacc gccccgacga aatcagccat
     2041 gtcgatctct cctttccttg cctaggtacc tcagggcgcc gcaggcgctt gatgtcaccg
     2101 ggtttctgcc gagacctgcg agtcagagcc gtcagactgc tccggaagcc gtcctacctc
     2161 gtctgggccc tcggcgggca ggaagcgtcc gatcagagcg tcgtagatgc cgacgccgac
     2221 gatgccgccg atgagggggc cgatgatcgg tacccagaag tagagattcc cgtattggtc
     2281 cctaaatgcg gtgccgtacc cggtcaggaa tgacgcgagc cgcggaccga agtcacgagc
     2341 cgggttgatc gcatagcccg cgtcggtgcc ccacgccatg ccgatcgcta ccacgagcag
     2401 gccgataacc actggcgcga ggttggcggc cggagcagtg tttcgggtgt cggtgatcgc
     2461 gacgataagg aacagcagga tcgcggtgcc aatgacctgg tcccggaacg cgcccgattc
     2521 atgcacgggc aatacgccgt taccggggag cgtggagaag atgccctggc tcttgatcgt
     2581 gtgttttggg tccagcttgg cgatcacctc ggtgtagtcc cagcgcacga tcagtgcggc
     2641 cacgaacgcc cctgccgttt gcgccagcgc gtacgggccc accttgcgcc acggaaaccc
     2701 gcggaataca gcgaacgcga cagtgacggc cgggttgatg tggccaccac tgagcttgcc
     2761 cgcggtgtag acgccgagtg cgacgccgag cccccatgcc caagcaatcg agtcgtgatc
     2821 tccgatacca gctgcggcga cctgtgccga gaccccgacc ccgagcacga tcaggatgaa
     2881 tgttcctgcg aactctgagg ccagttcccc catcaaaccg cgggatctga gccgttccgc
     2941 catgattcct cctgttgatc ggctcgactc cgggcaagga gcggagccca acgaatggtc
     3001 gattgcccgt tagccctggg gcggattgtc ggcgtactcg gccaccgcgt cggcattgcc
     3061 gaacgctagc cggggatgtc gtcgcgccgc tacacgtacc cgctatcgca cggtccttag
     3121 ggtcacgccg agttgggcat ctgagaaggg caggctctcg acacgttgca gcttgtgggc
     3181 ttcgtcgaga agacaaggaa agcgggagtg gaaacgggca gttcggcgga cgtttgactg
     3241 gggtcgtggt ctacggtttg ggcgagccga cgcgttggga ggatcgcctg catgctggct
     3301 ggccatcacc ccgccgacga ttgggcggcg ctggacagtg cccggtacta cgcgtcggcc
     3361 aggttgggag tgcgcggctg gcatgttggg ggagtcggac gtcggctctt ctttgctgtg
     3421 atacatccgc atctggtgcc cgcgtattac cgcttgttgc ggaagctccc ggttgagacc
     3481 atggtgctgt cggcgctgcc cgcaggcagg ctgctgtacg accgatggtc gcggcagtcg
     3541 tggcgaaagg cagcgtcaca cgcctgtctg ctgttgccgt cggacgccga cagctacctg
     3601 cggggacgac atcggcaggc cgttcgcacc aacctgcaca gggcggacaa gattcgcctg
     3661 acctgccggc ccattcccac cgactggggc gagacaaagc ggtctctgca tcgcggtacg
     3721 ttcgccagcc tcgtgaagga gatcgaccgg atcgagcagc gtgacgcggt cgtcaaatcg
     3781 tgggcagtgt tcgattcggg aggccaggta ctcggccggg cggtcgcgct cgtcgacgag
     3841 cggaccgccg tcctcttgct gctgcacggc ccaatggatc tcagcgttgc gcatcagact
     3901 cgctacctgc tccacacggc cgtcgtcgcc gatctcatca ggcgtggcgt gcgcaacctg
     3961 gtcattgagt cgattctcgg cgcgcctccc ggcctgaagt acttcgcggc ccggctcggt
     4021 tatcgcgcgt gtcggatcca ggtcgtcagc acaggtcgcg taccggccct gtcgtagcgc
     4081 tgccctacct gctgctgggc cccggcgggt tctgcgcagg cgtcagcgcg ccgtagcgca
     4141 agccgtccat caagagtgag acgagccgtc gcgcctggtc ttgccagccg acttggtcgc
     4201 tcaccatgca gatgccgccc agtgcgcgga tcaggtcgcc gccgtcgacg tcggaccgga
     4261 tccggccggt cgctgacgcg gcgtcgacca aggacttgat cgccgctcgg atccgctcgt
     4321 ggacgtaggc aaacagctcg gtgtggctgt cgtcggccga gagcttcaag gtggccgcca
     4381 ggccgcgctt ggtggcgaca tagccgacga agtcgcgcat ccacaactcg agcgccaggt
     4441 caggcggctg ctcggtgagt agttggtcgg cggcttcgca cagctgctcg acgccgtggc
     4501 ggtacgccgc ctcaacgagg gcgtcgcggg tcgggaagtg ccggtagagc gtcccgatgc
     4561 cgacgccagc tcggcgggcc acgtcctcga gtgaggccgc tgtgccgtgt tcggcgaatg
     4621 cctggccggc gcacacgagg agtgcctcgc gattgcgttt cgcatcggcg cgcagggccc
     4681 gttcggcggc gtcgggagtt gtcaccgcgc tcatgccagg cctcctcaga atcgacttgc
     4741 caaccggagg cagcctccgt atagttactg gaggcgccct ccgcttcatc catcatccac
     4801 caaggaggct gggaatgcca gtccactcac caccaagtcg cacggccgat ccgatcgcgg
     4861 tttcgccgcc ctcggagtcg aaatccggca accgccggtc ggccgcgctg gccatcatct
     4921 tgctcgccca actcatggtg gtgctcgacg caacgatcgt gaacatcgcc cttccgaaga
     4981 tccagactgc gctgtccttt agccgtaccg acctgtcctg ggtactcaat gcgtactcct
     5041 tgacgttcgg tgggctgttg cttctcggag cgcgcagcgg tgacctgttc gggcggcgtc
     5101 gcgttttcct cgccggcatc gccctgttta cggtcgcctc gcttgccggc gggttcacca
     5161 cgtcgtccgg cctgctgctg cttgcacggt cgatccaagg tgtgggagcc gcgttcgcct
     5221 cgccgtccgc cctggcgttg ctgatgatca catttcgtga aggcgccgag cgcacccgcg
     5281 cgatcgcgct gtacaccgca gtctcgatcg gcggcagtgc cgtcggccta gtcgccggcg
     5341 gggtccttgt cgagtgggtg tcatggcgct gggtcttctt cgtcaacgtg ccggtcggca
     5401 tcgtcctcct cgtcttggct cgccgtgact tgccggagac ggatcggcac accggacgcg
     5461 tcgacgtgcc aggtgcgctg acatcgacga tcgggatggc ggcgctggtg tacggcttcg
     5521 tccaggcggc cgcatccggc tggcaggcag gcagcacgat cgccgccttc acggtcggtg
     5581 tggttctgct ggcgacgttt gtgctcatcg agaccagggt ggccaccccc atcacacccc
     5641 tgcggctctt cgcggatcgc gaccgggtgt tctcctacct ggcacggctc gtgctggtcg
     5701 gcggcatgtt cgggatgttc ttcttcctca cccagttcct gcaagacgtg ctgggctaca
     5761 gcccgctgcg cagcggtctc gcgttcgtgc cactgaccat cgcgttgttc gccgcatcgc
     5821 aactggccgc gcgggtgctg ctcgcacggg tcggcccgaa gcggttgatg gttggcggct
     5881 tgacgacctc gacgatcggg ttgctcgtgc ttacccagat gtcggcgacc agcagctacg
     5941 gcctgattct ggccgggttg ttgctcttcg gcactgggaa cgggctggcg ttcgtgccgc
     6001 tgaccacggc gtcacttgcc ggcgttgacc ccaaggatgc cggtgcggca tcggg
//
LOCUS       DATCBJ010000211         3967 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_218171, whole genome shotgun sequence.
ACCESSION   DATCBJ010000211 DATCBJ010000000
VERSION     DATCBJ010000211.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 3967)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3967)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3967
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_218171"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            206..949
                     /gene="cofC"
                     /locus_tag="VII50_04290"
     CDS             206..949
                     /gene="cofC"
                     /locus_tag="VII50_04290"
                     /EC_number="2.7.7.68"
                     /inference="COORDINATES: protein motif:HMM:NF014085.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043814 - phospholactate
                     guanylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-phospho-L-lactate guanylyltransferase"
                     /protein_id="HEY5050097.1"
                     /translation="MNTSIPDDVHWVVVIPAKPLGTAKSRLADAAGSRRPELALAMLL
                     DTVEAALPVDEVAAVLVVTDDDLIAAAASALGAVVVPDLPGGGLNAAFQYGIEVAAAR
                     YPGTGVALLAGDLPALRTHELEAALAVAASSPGVVAVADRDELGTTMLAARLPPQLRL
                     AFGVGSFARHRALDAVALELEGLDSLRCDVDDAVGLRAAISLGVGKRTADMTKGWDPL
                     VVGPTSSEGREAKQSKEEVGPKTKGSQPL"
     gene            complement(1234..1491)
                     /locus_tag="VII50_04295"
                     /pseudo
     CDS             complement(1234..1491)
                     /locus_tag="VII50_04295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008214712.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
     gene            complement(1635..2234)
                     /gene="leuD"
                     /locus_tag="VII50_04300"
     CDS             complement(1635..2234)
                     /gene="leuD"
                     /locus_tag="VII50_04300"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009074083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HEY5050098.1"
                     /translation="MEKFTTHTGRGLPLRRSNVDTDQIIPAVYLKRVTRTGFEDGLFA
                     AWRADPDFVLNLPRYAGADVLVAGPDFGTGSSREHAVWALQNYGFKAVISPRFADIFR
                     GNSLKGGLLTVLISESDVEAMWAVIEADPQTQITVDLVAREVRYADVVLPFEIDDYTR
                     WRLMEGLDDIGLTLRHVDDVDEFESHRPAWKPRTLPSAV"
     gene            complement(2270..3670)
                     /gene="leuC"
                     /locus_tag="VII50_04305"
     CDS             complement(2270..3670)
                     /gene="leuC"
                     /locus_tag="VII50_04305"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HEY5050099.1"
                     /translation="MGATMAEKVWDEHVVRHADGEPDLVYVDMHLVHEVTSPQAFDGL
                     RVAGRPVRRPDLTIATEDHNVPTTDIDQPIADPVSRTQVETLRHNCAQFGIRLYPMGD
                     ASQGIVHVIGPQLGLTQPGMTIVCGDSHTSTHGAFGALAFGIGTSEVEHVLATQTLPQ
                     YRPKTMGITVNGILPDGVTSKDIILAVIAKIGTGGGQGYVIEYRGEAIRALSMEARMT
                     VCNMSIEAGARAGMIAPDETTFAYLKGRPHAPTGDDWDAAVAHWRSLVTDDDATFDVD
                     VVLDAAQLSPFVTWGTNPGQGAPLGSSVPDPSTIADAGERGSVERALEYMGLQAGTKL
                     RDIKVDTVFLGSCTNGRIEDLRAAAAVLKGRKVADTVRVLVVPGSMAVKAQAESEGLD
                     KIFTEAGAQWRSAGCSMCLGMNPDQLAPGERSASTSNRNFEGRQGKGGRTHLVSPLVA
                     AATAVTGHLSAPSDLD"
     gene            3734..>3967
                     /locus_tag="VII50_04310"
     CDS             3734..>3967
                     /locus_tag="VII50_04310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004983042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEY5050100.1"
                     /translation="MDESSGVGVLDKSVAVLSALEAGPASLASLVTLTGLPRPTAHRL
                     AVALERHRLVARDSEGRFVLGPRLGELATAGGED"
BASE COUNT          640 a         1325 c         1269 g          733 t
ORIGIN      
        1 agccacgcca ggtcggcttg gtcgacatcc acaagaacgg cttcgcgatc gccgcgcaca
       61 cgcgaaacca cctaaggtgt tcgcctgatc gcacccgctg ctctcctgtc atcgaccggt
      121 gacaccggcc gacggtgtcg ctgcaccagt gtcccgtgca ggcctcggca gtgtcgagtc
      181 ggaccgccgg gtagcgtccg caccgatgaa cacatcgatt ccggacgacg ttcactgggt
      241 cgtcgtcata ccggccaagc cccttggtac ggccaaatca agactggcgg acgccgccgg
      301 cagccgccgt cccgaactgg cccttgcgat gctcctcgat acggtcgagg cggcgttgcc
      361 cgtcgacgag gtagccgcgg ttctggtggt cactgacgac gacctgatcg cggctgcggc
      421 gtccgcgctc ggagcagtcg tcgttccaga tctgccaggc ggtgggctca acgccgcctt
      481 tcagtacggc atcgaggtgg ccgcagcgcg atatcccggc accggcgtcg cgctgctcgc
      541 gggggaccta ccggccctgc ggacgcacga gctcgaggcc gcccttgccg tcgccgcatc
      601 gtcgcccggc gtggtcgcag tcgccgatcg cgacgagtta ggcacgacaa tgctggcggc
      661 gcgtttgccg ccgcagctgc ggctcgcgtt cggcgtggga tcgttcgcgc gccatcgcgc
      721 cctcgatgcg gtcgcgctgg aactcgaggg actcgacagt cttcggtgcg atgtcgacga
      781 cgcggtcgga ctgcgcgcgg cgatcagcct cggcgtcggt aagcgaacag cggacatgac
      841 gaagggctgg gatcccttgg tcgtagggcc aacgtcgtcc gaaggacgtg aagcgaagca
      901 gtccaaggaa gaggttggcc cgaagaccaa gggatcccag cccctctaac ttcaggcttt
      961 cttggcgacc cgcttgacgg gagccttctt cgcgggtgcg gccttcttga ccgcggccct
     1021 tgccggtgca gccttcttgg ccggcgcggc cttccgggcg ggtgccgcct tcttgaccgc
     1081 ggccttggcc ggtgcggcct tcttggccgg tgcggccttc ttcactgctg ccttgaccgg
     1141 tgcggccttc ttgaccgcag ccttgaccgg tgcggccttg gcaggcgcag ctttcgccgg
     1201 cgcagccaac ttcactgtgc cgctgacgac gcccttgagg tgcgtgcccg gccgaaactt
     1261 cggcacggag gtcttcttga ccttgacccg tgcgccagtc gctgggttgc gggcaagtcg
     1321 cgcggcccgc tcgaccttct cgaaaatacc gaatccagtg atggcgacct tctcgccctt
     1381 cgccactgtt ctggtgatgg tgtcgatgac ggcctcaacc gcctcgcctg ctgctttctt
     1441 gttgtcgccc aggcgagtgg cgatggagtc gatcagttcc gccttgttca cgtgctcctc
     1501 cgaatgatgt tgtcgagtgc acacccgact cggggtgaac ctaggccccc ggcgcgctca
     1561 aaacaacgac cgcgaagaag aatttccttg tgtcgcggaa aatctgggct aaaacccggc
     1621 ccatgaccca tccgtcacac tgctgatggc agtgtccgcg gcttccaagc cggccggtgc
     1681 gactcgaatt cgtcgacatc atcgacatgc cgcaacgtca gaccgatgtc gtcgagaccc
     1741 tccatgagcc gccaacgcgt gtagtcgtcg atctcgaaag gcaagaccac atctgcgtag
     1801 cgaacctctc gggcgacaag atcgacagtg atctgcgtct gcggatcggc ctcgatcacc
     1861 gcccacattg cttccacgtc ggattcgctg atcagtacag tcagcaagcc acccttgagc
     1921 gagtttcccc tgaagatgtc ggcgaacctt ggtgagatga cggccttgaa gccgtaattc
     1981 tgcagcgccc agaccgcgtg ctctcgcgat gatccggtcc cgaagtcagg gcccgcgacg
     2041 aggacgtcag cgcccgcgta ccgcggcaga ttcagcacga agtcaggatc ggctcgccat
     2101 gctgcgaaca gcccgtcctc gaagccagtg cgggtgaccc gcttcaagta gacggccggg
     2161 atgatctggt cggtatcaac gttgctgcga cgcagtggca ggccacggcc ggtgtgcgtg
     2221 gtgaatttct ccatcggaag tctcccggga ctagttgcgt gaacgtgggt cagtcgagat
     2281 cggacggcgc ggacaggtgc ccggtgactg cagtggccgc agcaacgagt ggcgacacca
     2341 gatgcgtgcg gccgcccttg ccttgccggc cttcgaagtt gcggttcgat gtggacgccg
     2401 accgctcgcc cggggcgagt tggtcagggt tcatgccaag gcacatcgaa caccccgcac
     2461 tacgccactg cgcgccggct tcggtgaaga tcttgtcgag gccttcggac tcggcctgtg
     2521 ccttgaccgc catcgaaccg ggcaccacga gaacgcgcac ggtgtccgct accttgcgac
     2581 ccttcaggac agcggccgca gcacgcaggt cttcgatacg cccgttcgta cacgacccga
     2641 ggaagacggt gtcgaccttg atgtcacgca gcttcgtgcc ggcctgcagg cccatgtact
     2701 ccagcgcgcg ttcgacgctg ccccgctcac ctgcgtcagc gatcgtcgac ggatcgggaa
     2761 cgctggagcc aagtggcgct ccttgacccg gattggtgcc ccaggtgaca aacggcgaca
     2821 actgcgccgc gtccagcacg acatcgacat cgaacgttgc atcgtcgtcg gtaaccaagc
     2881 tccgccagtg agcgaccgca gcatcccaat cgtcgccggt cggagcatgc gggcgaccct
     2941 tcaagtacgc gaacgtcgtc tcgtccgggg cgatcattcc ggcccgcgcg ccagcttcga
     3001 tcgacatgtt gcacacggtc atgcgggctt ccatcgacag cgcgcggatc gcctcgccgc
     3061 ggtattcgat gacgtagccc tgcccgccgc cggtaccgat cttcgcgatc accgcgagga
     3121 tgatgtcctt gctcgtcacg ccgtccggca ggatgccgtt caccgtgatc cccatggtct
     3181 tggggcggta ctgcggcaag gtctgggtcg caagcacgtg ctcgacttcg ctggtgccga
     3241 tgccgaatgc cagagcgccg aacgcgccgt gggtcgaggt gtgtgagtca ccacacacga
     3301 tggtcatgcc tggctgcgtg agaccgagct gcgggccgat gacgtggacg atcccctgtg
     3361 atgcatcgcc catcgggtag agccggatcc cgaactgcgc acagttgtgt cgcagcgtct
     3421 cgacctgcgt gcgtgatacc gggtcggcga tcggctgatc gatgtcggtg gtcggcacgt
     3481 tgtggtcttc ggtcgcgatg gtgaggtcgg gccggcgcac tgggcggccg gccactcgca
     3541 acccgtcgaa cgcctgcggg ctggtgacct cgtggacgag gtgcatgtcg acgtacacca
     3601 ggtccggctc gccgtcagcg tgccgcacca catgctcgtc ccagaccttc tccgccatcg
     3661 tcgcgcccat gagcccgctc cacttcgctg agaaaaactt gcgtctcgca tattgagacg
     3721 gcagtatcag tacatggacg agtctagcgg cgttggcgtt ttggacaagt cggttgccgt
     3781 actttccgct ctcgaggccg gccccgcctc gctggccagc cttgtcacac tcaccggcct
     3841 gccgcgacca accgcccatc gcctcgcggt tgccctcgag cgacaccggc tggtggcccg
     3901 agacagcgaa ggcagattcg tgctcggccc acgactcggg gagttggcga ccgcgggcgg
     3961 tgaggat
//
LOCUS       DATCBJ010000212         3177 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_350475, whole genome shotgun sequence.
ACCESSION   DATCBJ010000212 DATCBJ010000000
VERSION     DATCBJ010000212.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 3177)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3177)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3177
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_350475"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..502
                     /locus_tag="VII50_04315"
     CDS             <1..502
                     /locus_tag="VII50_04315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004808724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY5050101.1"
                     /translation="HAVAFAPAAALGGNFLNTGWDDVATALHVSAYSLKALTTAALPL
                     MPTGGSVVGLDFDASVAWPLYDWMGVAKAALESCSRYLARDLGKSNVRVNLVSAGPIR
                     TMAAKGIPGFDELAAIWETRAPMAWDVTDAEPAARAIVALLSDWFPKTTGEIIHVDGG
                     VHAMGS"
     gene            563..1516
                     /locus_tag="VII50_04320"
     CDS             563..1516
                     /locus_tag="VII50_04320"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HEY5050102.1"
                     /translation="MSAEQSRNARRQQRLAIAIVLNVAVVVAQIIWGLLAHSLGLLAD
                     AGHNLADVAGLAIAVIALRYSLRSATMARSFGHHRATILAALANGGLLLAVTGFVAID
                     AVVRLVHPRPVHGGIVALVALCAAALNAVGATLVHERRARPAAHADLNMSAATLHLVS
                     DAAVSLAVAVAGLVILVSHGTYWLDPAVSLVVCVAITSQAVRLLHRSADVLLESTPKA
                     IDPEAVLAAVRAVPSVVDVHDLHVWSLSSDLHAMSAHVAVSGHPTLEQAQATGEWIKN
                     LVADQFDIAHATIEMECEACPEPDPCALETLEPVRASGLHH"
     gene            1672..2727
                     /gene="ppk2"
                     /locus_tag="VII50_04325"
     CDS             1672..2727
                     /gene="ppk2"
                     /locus_tag="VII50_04325"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR03707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006793 - phosphorus metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 2"
                     /protein_id="HEY5050103.1"
                     /translation="MATTRTKSESPRRDHLAGRERRKVAWDSVRLTEDLDALTLEADN
                     EDLDDPILVGPDGKRVDTWRENYPYEERLERGEYERQKRALQVELLKMQEWIQDHGQR
                     VCVLFEGRDAAGKGGTIKRFMEHLDPRAARIVALGKPTEREIGEWYFQRYIAQLPSAG
                     EIVLFDRSWYNRAGVERVMGWTTDEKYQLFLKQAPVFERLQTDDGLILIKLWFSVSRG
                     EQRTRFIIRRIDPVRQWKLSPTDLALIDRWDDFTEAKAAMFEVTDTDNAPWTVIRSND
                     KRRARVAAMRQVLSRVPYPDKDPTVVGKPDSRIVGPPWKIYEHDERKIHRPPGRTSAK
                     TPDPSSIDARVLDGAGK"
     gene            complement(2795..3025)
                     /locus_tag="VII50_04330"
     CDS             complement(2795..3025)
                     /locus_tag="VII50_04330"
                     /inference="COORDINATES: protein motif:HMM:NF019887.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HEY5050104.1"
                     /translation="MPLTQTIAIGDGANDLDMLGRAGLGVAFNAKPVVREAADTALNV
                     PYLDAILYLLGITRDEIDAADAEGVVHRTPTP"
BASE COUNT          536 a          996 c         1083 g          562 t
ORIGIN      
        1 tcatgccgtc gcgtttgcac ctgcagcagc gctcggtggc aacttcctga acaccggctg
       61 ggacgacgtc gcgactgcgt tgcacgtgtc ggcctattcg ttgaaggcgc tgacaacggc
      121 ggcgctccct ctgatgccca cggggggctc ggtcgtcggc ctcgatttcg acgccagcgt
      181 cgcgtggccc ttgtatgact ggatgggcgt ggcgaaggcg gcccttgagt cgtgctcgcg
      241 ctacctcgcc cgcgatctcg gcaagagcaa cgtgcgggtc aatctcgtct cggccggacc
      301 gatccgaacc atggcagcta agggaattcc cggattcgac gagctcgctg cgatctggga
      361 gacgcgggct ccgatggcgt gggacgtgac tgacgccgaa ccggccgcca gggcgatcgt
      421 cgcgctgctg tcggactggt tccccaagac cacgggcgag atcatccacg tcgatggcgg
      481 cgtccacgcc atgggcagct gatcggcgtc ccgccctgcg ctccgcgtcc cgctcgaggc
      541 tatttcgcgt accgttgatc acgtgagcgc cgagcagagc cgaaacgccc gccgccagca
      601 gcggctggcg atcgccatcg tgctcaacgt cgcagtcgtg gtggcgcaga tcatctgggg
      661 actgctcgcc cattcactcg ggctgcttgc tgacgcgggg cacaatctcg cggacgtcgc
      721 ggggctggcc atcgccgtga tcgcgctgcg gtactcattg cggagcgcca cgatggcccg
      781 ttcgttcggc catcaccggg ccaccatcct ggcggcgttg gccaacggcg gcttgctgct
      841 cgccgttacc gggtttgtgg ccatcgatgc agtggtcagg ctcgtgcatc caaggcccgt
      901 gcacggcggc atcgtcgcgc tggtcgccct ttgtgccgcc gcgttgaacg cggtcggcgc
      961 gacgttggtc catgaacgcc gcgctcgccc cgctgcgcac gccgacttga acatgtcggc
     1021 cgcgaccctg cacctcgtct cagacgcggc tgtctcactc gcggtggccg tggccggcct
     1081 cgtcatcctg gtcagccacg gcacctactg gcttgacccg gcggtgtcgc tggtggtgtg
     1141 tgtggcgatc accagccagg ccgtgcggct gctccatcgc agtgcggacg tgttgctgga
     1201 atcgacaccc aaggcgatcg atccggaggc tgtgcttgcg gccgtgcgcg cggttccgag
     1261 tgtcgtggac gtccacgatc tgcacgtgtg gagcctgtcg agcgacctgc acgctatgtc
     1321 agcgcacgtc gctgtttctg gccatcccac cctcgagcag gcccaggcga cgggggagtg
     1381 gatcaagaat ctggtcgcgg atcagttcga catcgcgcac gccacgatcg agatggaatg
     1441 cgaggcctgt cctgagcctg atccgtgtgc ccttgagacc ctcgaaccgg tcagggcgtc
     1501 ggggctgcat cactgacggg tcctagccgc tctggcctgc gcgttcgcgg acacgctcga
     1561 tgcgggcggg caggagactt cacccatcgt tcatgatcgc gacacctcgg gtggcattgc
     1621 gcacgctgcc gatacggcag attctcgggg cagcgatcga aagggcggca catggcaaca
     1681 acgcgaacca agtcggagtc accacgccgg gaccacttgg ccggccggga gcggcgcaag
     1741 gtcgcttggg acagcgtgcg gctcaccgag gatctggatg cgttgacgct ggaggctgac
     1801 aacgaggacc tggatgaccc gatcctggtg ggccccgacg ggaagcgcgt cgacacctgg
     1861 cgggaaaact acccctacga ggagcggctc gagcgcgggg agtacgagcg acagaagcgg
     1921 gcgctgcagg tcgagctgct caagatgcag gagtggatcc aggaccacgg gcagcgcgtg
     1981 tgtgtgctgt tcgagggccg tgacgcggca ggcaagggcg gcacgatcaa gcggttcatg
     2041 gagcacctcg acccgagggc agcacgcatc gttgcactgg gaaagccgac cgaacgcgag
     2101 atcggtgagt ggtatttcca gcgttacatc gctcagctgc ctagcgccgg cgagatcgtg
     2161 ttgttcgacc gttcctggta caaccgggca ggcgtcgaac gggtgatggg ctggacgacc
     2221 gacgagaaat accagctgtt tttgaagcag gctccggttt tcgagaggct gcaaaccgac
     2281 gacggtttga ttctcatcaa gctgtggttc tcggtctctc ggggcgaaca acgcactcgg
     2341 ttcatcatcc ggcgcatcga tccggtgcgg cagtggaaac tgagtccgac cgatctagcg
     2401 ctgatcgacc gctgggacga cttcaccgag gcgaaggcag cgatgttcga ggtgaccgac
     2461 acagacaacg cgccgtggac ggttatccga agcaatgaca agcgacgagc ccgagtggcc
     2521 gcgatgcgtc aggtgcttag tcgggtgccg tatcccgaca aggatccgac agtagtcggg
     2581 aaaccggatt cgcgcattgt gggtccaccg tggaagatct acgaacacga cgagcgcaag
     2641 atccatcgtc cgcccggccg aacctctgcg aagacgccgg acccatcgtc gattgacgcc
     2701 cgcgttctcg atggtgcggg gaagtagctt cgccgacggt cttggccgct caggtcactc
     2761 tggtcgcagg cctctgtgaa acctcatgac gcgattatgg cgtcggcgtc cggtgcacta
     2821 ctccttcggc gtctgccgca tcgatctcgt cacgcgtgat gccgagcagg tagaggatcg
     2881 catccaggta gggcacattc agcgcggtat ctgcggcctc gcgcacgact ggcttggcgt
     2941 tgaacgcgac cccaagtccg gcacgcccga gcatgtcgag atcgttggcg ccgtcgccga
     3001 tcgcgatcgt ctgggtgagt ggcacaccgg ccgaggcggc gaaacgttgg agtgcagcgg
     3061 ccttaccggg cggtcgacga tcggcccgac caacgttccg gtgagcagac catcgacgac
     3121 gtcgagggtg ttggcggcag cgaaatcgag gccgagctcg tcgacaagac cgtcggt
//
LOCUS       DATCBJ010000213         3852 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_43007, whole genome shotgun sequence.
ACCESSION   DATCBJ010000213 DATCBJ010000000
VERSION     DATCBJ010000213.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 3852)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3852)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3852
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_43007"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..285)
                     /locus_tag="VII50_04335"
     CDS             complement(<1..285)
                     /locus_tag="VII50_04335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HEY5050105.1"
                     /translation="MSATEIDEYLEQLDEPKRSTLEQLRRDILGVVPDAEQCLSYSVP
                     GFKVDGKTIAGFAAFKNHLSYLPHSGSVFPELADELVGYQKSTGALRFAID"
     gene            complement(285..569)
                     /locus_tag="VII50_04340"
     CDS             complement(285..569)
                     /locus_tag="VII50_04340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009077648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1905 domain-containing protein"
                     /protein_id="HEY5050106.1"
                     /translation="MELRKRFTATLEKSPAKGGWTYVVMPGSAEYFGTRGLVKVRGLV
                     DGHPFESSFMALGDGRHKLPIKAELQTAIAKKPGDQVTVVLHERVEQGGT"
     gene            complement(667..1053)
                     /locus_tag="VII50_04345"
     CDS             complement(667..1053)
                     /locus_tag="VII50_04345"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HEY5050107.1"
                     /translation="MRTILDTSVLLGPDPGPVEGEVAISAVSLAELHFGVLAAHSDAV
                     RAERLRRLAVIERTFDALPVDEIVAREYGRLAAAVIAAGRKPRARMADLLIAATAAAH
                     DATLWTRNPGDLLGVDNLVRVHTPTP"
     gene            complement(1050..1295)
                     /locus_tag="VII50_04350"
     CDS             complement(1050..1295)
                     /locus_tag="VII50_04350"
                     /inference="COORDINATES: protein motif:HMM:NF014643.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system
                     prevent-host-death family antitoxin"
                     /protein_id="HEY5050108.1"
                     /translation="MTTTGLRELRQQASELVRQAEAGQTVTITVSGREVAELGPIRRH
                     RWRRGSDIGEVFRGPSDDDWAADRDAIAQTVVDPFTR"
     gene            complement(1384..2511)
                     /locus_tag="VII50_04355"
     CDS             complement(1384..2511)
                     /locus_tag="VII50_04355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5050109.1"
                     /translation="MGPPFVALLAGWSLIASRAPKGWTSVFNPATFNHWDAGQYLSIA
                     KHGYRASTHCNIAPTVHLCGNITWFPGYSGLIRLLVSAHIGYLAAALAIAWICWYLTL
                     LMVWVLSAERNRGGTSRGVACLALAAVFPGQVYFAALFPISLATFAVLLCIWLATRTS
                     FATRRATAVRTSIAGIVAGLAYPLAFAAAPALVVTAAMARTRKARTAMLIGAAAIVAG
                     YALVLGYAQVTVGKWDAYFISEREEYGVRAHNPLVLLTSRYHQLVHPSSSTWRTITQQ
                     GALATLLVVVAIVVTVPVLIRARRAADLTDIALLVTACIAWLVPYVGAGALSIYRSEA
                     CLIVLVPLLRRLPVWVIAIAACAAAVIAFNMAPLFFSNALM"
     gene            2788..3669
                     /gene="rsmI"
                     /locus_tag="VII50_04360"
     CDS             2788..3669
                     /gene="rsmI"
                     /locus_tag="VII50_04360"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009083463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HEY5050110.1"
                     /translation="MPSPDSPSLGELVIAGVPLGNPGDASARLRSALQSAEVVAAEDT
                     RRLRRLAADLDVHLGGRIVSYFEANEAARTGELVADLLQGRRVLLVTDAGMPSISDPG
                     YRLVVAALAAGVGVTCLPGPSAVTTALALSGLPVDRFCFEGFLPRRGGARAARLLSLA
                     HEPRTMVFFEAPHRLGSCLADLASAFGESRPAAVCRELTKTYEEVRRGPLSELVAWAA
                     AGVRGEITIVVAGATLAVASFDAASLADLVSDAQDAGMPRKEAIAAVASQTGASRREV
                     FDALVARKTSEAPGVIG"
BASE COUNT          611 a         1189 c         1348 g          704 t
ORIGIN      
        1 gtcgatggcg aagcgcaatg ctccagtgga cttctggtac ccgacgagct catcggctag
       61 ttcaggaaac accgagccgc tatgggggag gtagctcagg tggttcttga acgccgcgaa
      121 cccggcgatc gtcttgccgt cgaccttgaa accgggcacc gaataggaca ggcactgttc
      181 ggcatctgga accacgccaa ggatgtcgcg gcgcagttgt tccagggtcg agcgtttcgg
      241 ctcgtccagc tgctcaaggt attcgtcgat ctcggtggcc gacatcaggt gcctccctgc
      301 tcgacgcgct cgtgaagcac cacggtgacc tggtctccgg gcttcttggc gatagccgtc
      361 tgcagctccg ccttgatcgg cagtttgtgt cggccgtcgc ctagcgccat gaaggaactc
      421 tcgaacgggt ggccgtcgac gaggcctcgc accttgacga gtccacgggt accgaagtac
      481 tccgccgacc cgggcatcac gacataggtc caacctccct tggcgggact cttttccagg
      541 gtggccgtga atcgtttgcg aagctccatc gcacctccac ctcgacggta ctcgttggaa
      601 tggacgacac agggcggcgg atttcatcgg tgtcgtgtat gcatcggccg acactggcgt
      661 gccgagtcag ggcgtcggcg tgtgcacccg cacgaggttg tcgaccccca gtaggtcacc
      721 gggattccgc gtccacaacg tcgcgtcatg cgcggcggcg gtggcggcaa tgagcaagtc
      781 ggccatccgg gcacgcggct tgcggccagc ggcgatgact gcagcggcca agcgaccata
      841 ttcgcgcgcc acgatctcgt cgacgggcaa cgcatcgaag gtgcgctcga tgaccgcaag
      901 ccgccgcagc cgttctgctc tgacggcatc cgaatgcgcg gcaagtactc cgaaatgcag
      961 ctcggccagc gacactgcgc tgatcgcgac ctcgccctcg acgggcccgg gatctggtcc
     1021 gagtaggacg ctcgtgtcga ggatcgttct catcgggtga agggatcgac aacggtctgc
     1081 gcgattgcat cgcggtcggc tgcccagtcg tcgtcggacg ggccccgaaa cacctcacca
     1141 atgtctgatc ctcgtcgcca acggtgtcgg cggatcgggc cgagctcggc gacttctcgt
     1201 ccgctgaccg tgatcgtcac cgtctggcca gcctcggcct ggcgcaccag ttcactggcc
     1261 tgctggcgca actcacgcaa tccggtcgtc gtcataggct caatgtagcg catgtgctac
     1321 gcccgcgcaa tcggcgtgcc gcaacggtgg acgtaccaac ctgatggccc gcgcggccgc
     1381 aagttacata agtgcgttgg agaagaacag cggcgccatg ttgaaggcga tcaccgcggc
     1441 ggcgcacgcc gcgatggcga tcacccagac cggcaaccgg cgcagcagcg ggacgagcac
     1501 gatcaggcac gcttccgatc ggtagatgct cagcgcgcct gctccgacat aggggaccag
     1561 ccaggcgatg caggcggtta cgagcagcgc gatatcggtg agatcggctg cgcgtcgggc
     1621 ccgtatgagc accgggacgg tgacgacgat cgccacgacg acgagcaacg tggcgagcgc
     1681 accctgctgc gtgatggttc gccacgtcga cgaactcggg tgcacgagct ggtgatagcg
     1741 cgaggtcagc aggacgagcg ggttgtgcgc gcggacgccg tactcctctc gttcgctgat
     1801 gaagtaggcg tcccacttgc cgaccgtgac ttgtgcgtag cccagtacca gcgcatagcc
     1861 ggcgacgatg gcggccgcgc cgatgagcat cgccgtgcgt gctttgcggg tgcgagccat
     1921 cgccgcggtt acgaccagtg ctggagcggc ggcgaaggcg agcgggtacg cgagtccggc
     1981 aacgatgcca gcgatgcttg ttcggaccgc ggttgccctg cgtgtagcga acgatgtgcg
     2041 agtcgccagc cagatgcaca acaacacggc gaaggtcgcc agcgagatcg ggaacagcgc
     2101 ggcgaagtag acctggcccg ggaacacggc ggcgagtgcg agacaggcga cacccctcga
     2161 cgtcccgcct cgatttcttt ctgcactgag cacccacacc atcagcagcg tcaggtacca
     2221 gcagatccat gcgatcgcga gggcggccgc gaggtagccg atgtgggccg agacgagtag
     2281 tcgaatgagg cctgaatagc cggggaacca ggtgatattg ccgcacaagt gaaccgtcgg
     2341 cgcgatgttg cagtgcgtgg aggcgcggta gccgtgcttg gcaatgctga ggtattggcc
     2401 cgcgtcccaa tggttgaacg tcgcgggatt gaagacgctc gtccagcctt tgggggcgcg
     2461 actggcgatg agcgaccagc ccgcgaggag tgcaacgaaa ggcgggccca agagtatgag
     2521 cgcgttccgt gcccgacttc ggaccgcttc gtcgggggtc gtcgccgcgg gcgttggtgc
     2581 gagttgggta gcggtcacat tgatgggact gtgtgcgaac ggtcccaggt tccgcgcaat
     2641 tgtctcggtc tggcaacgaa atttggtcgg gtcactgtgg tcacggcttt tcacacgttc
     2701 ccgctttgag cgcgcttgaa cctgcctttg tgaggaatcg cggtcggacg cgaccggctg
     2761 acgaaggcgt gctggtttga tggcgtcatg ccgtcgccgg attcaccgtc gcttggcgaa
     2821 ctcgtgattg cgggcgttcc gctcggcaat ccgggcgatg cgagtgcgcg gctgcggtcc
     2881 gcactccaga gcgccgaggt cgttgcggcg gaggacactc gccggctgcg gcgtttggcg
     2941 gctgacctcg acgtccatct tggtgggcgg atcgtgtcct acttcgaagc caacgaggcc
     3001 gcgcgcaccg gcgaactcgt cgcggatctg ttgcaggggc ggcgggtgct gctcgtgacg
     3061 gacgccggga tgccgtcgat ttccgatccc ggttaccggt tggtggtggc tgcgcttgcc
     3121 gccggtgttg gcgtgacctg tctgcccggg ccgtccgcgg tgacgacggc gttggcgctg
     3181 tcgggcctgc cggtcgaccg cttctgcttt gagggattct tgccgcgccg cggtggtgcg
     3241 cgtgctgctc ggctgctgtc gttggcccac gagccgcgca cgatggtgtt cttcgaagcc
     3301 cctcaccggt tgggctcgtg tttggccgac ctcgcgtcgg cgttcgggga gtcgcggcca
     3361 gccgcggtgt gccgcgagct gaccaagacc tacgaagagg tgcgccgcgg cccgctctcg
     3421 gagttggtcg catgggccgc cgccggggtc cgcggcgaga tcacgatcgt ggtcgccggt
     3481 gcgacccttg ctgttgcgtc gttcgacgcc gcctccctcg cggacctggt ctccgatgcc
     3541 caggacgccg ggatgccgcg caaggaagcc atcgccgccg tcgcttcgca gacgggtgcc
     3601 tcccgccgtg aggtgtttga cgcactggtc gcccggaaga cgtctgaggc acctggtgtg
     3661 atcggatgac atgatgggag cggttgacgc gatcacgggg aggggcatgt tcagggcatt
     3721 cgtagccggg ttcttggcgg agtttgcccg cctgcgtgtc gtcgtgacga cccgctggcg
     3781 agcgtacggc ctgcggggat tcgtgctgac tgctgtggtc tcggcggcga tcatcgtggc
     3841 ttggctggtc ga
//
LOCUS       DATCBJ010000214         2012 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_398032, whole genome shotgun sequence.
ACCESSION   DATCBJ010000214 DATCBJ010000000
VERSION     DATCBJ010000214.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2012)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2012)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2012
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_398032"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1165)
                     /locus_tag="VII50_04365"
     CDS             complement(<1..1165)
                     /locus_tag="VII50_04365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NRAMP family divalent metal transporter"
                     /protein_id="HEY5050111.1"
                     /translation="MTTDPSRDGTPGTPSPAGAEPSPAPAASTPSSLESATIPGRAPP
                     TPAPRPPARERVTLDSAHIGDIRGAFGTIGQHEEAHHGLRARSLALLAIVGPGLIVMV
                     GDNDAGGVATYSQAGQNYGTSLLWVLILLVPVLIVNQEMVVRLGAVTGVGHARLINER
                     FGRFWGWFSVGDLFLLNFLTIVTEFIGVSLSLGYFGVSKFIAVPIAAIALVAMTASGS
                     FRFWERSMFVFVFANLLVVPLFFMAHPGIGDVAHDFVIPGIQGGANSTSILLIIGMVG
                     TTVAPWQLFFQQSNIIDKRITPRWINYERLDTWIGAIIVVVGAAALISVSAFAFGHTH
                     YFGRFTDAGATASGLDHVLGSIPAAFFAVVLLNASLIGAGALTLSTSYAFGDVF"
     gene            complement(1412..>2012)
                     /locus_tag="VII50_04370"
     CDS             complement(1412..>2012)
                     /locus_tag="VII50_04370"
                     /inference="COORDINATES: protein motif:HMM:NF015413.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEY5050112.1"
                     /translation="NDEHQVEFLRDRSDEEVAAVLGRMESDDAADLLLEIEQDRRIPV
                     LNLLSAAKQLKIKRLLGYNPSTAGGLMNPDFASLPANATVAQALSVVRGSELEPGQLL
                     TLWVIDADGHLAGGVLASELVRAAEHATVAELIETAVPTVSPETELPEVARLMADFNL
                     LAIPVIDTDEKPIGVVAVDDVIELLLPEGWRRRAGLARG"
BASE COUNT          361 a          670 c          676 g          305 t
ORIGIN      
        1 cgaacacgtc accgaacgca tagctcgtcg acagcgtcag cgcgccggcc ccgatcagcg
       61 acgcgttgag cagcaccacg gcgaagaacg cggcggggat cgagccgagc acgtggtcta
      121 ggccgctcgc ggtcgctccc gcgtcggtga accgcccgaa atagtgcgtg tgtccgaagg
      181 cgaacgccga cacggagatc agggccgctg cgccgaccac gacgataatc gcgccgatcc
      241 aggtgtcgag gcgttcgtag ttgatccagc gtggggtgat ccgcttgtca atgatgttgg
      301 actgctgaaa gaacagctgc cagggcgcga ccgtcgtgcc gaccatgccg atgatcagca
      361 ggatcgacgt tgagttcgcg ccgccttgga tgcccgggat gacgaagtcg tgggcgacgt
      421 cgccgatgcc cgggtgggcc atgaagaaca gggggacgac gagcaggttg gcgaacacga
      481 acacgaacat cgatcgctcc cagaagcgaa agctgccgct ggcggtcatc gccaccagcg
      541 cgatcgccgc gatcggcacg gcaatgaact tcgagacgcc gaaatagccc agtgacaggc
      601 tgaccccgat gaactcggtg acgatcgtca ggaagttcaa caggaacagg tccccgactg
      661 agaaccaacc ccagaaccgt ccgaagcgct cgttgatcag tctggcgtgc ccgacgccgg
      721 tgaccgcacc gaggcggacg accatctcct ggttgacgat caaaaccggg acgagcagga
      781 tcagcaccca cagcaggctg gttccgtagt tctggcctgc ctgggagtac gtggcgaccc
      841 caccggcgtc gttgtcgccg accatcacga tcagcccggg cccaacgatc gcgagcaacg
      901 cgagtgaccg ggcccgcaag ccatggtggg cctcctcgtg ctgcccgatc gtcccaaacg
      961 ccccccggat gtcgccgatg tgcgcggagt ccagggtcac ccgttcgcgc gccggcgggc
     1021 gtggcgcggg ggtggggggc gcgcggcccg ggatcgtcgc ggactccagg gatgaaggcg
     1081 tgctggccgc gggtgccggg ctcggctcag cgccagcagg tgaaggcgtg ccgggcgtgc
     1141 catcccggct tgggtcagtc gtcatctgtg acctcctttc cgatccggtc gcagcttccg
     1201 cagcggccgt cgagaaggtc ctcagcccga gatcatgcct cgggacgcga gcggacgttc
     1261 accacggcgg ccacggaccg gctacttcgc ggcagctcgc tgtcgggata taagccttgc
     1321 tcgtccattg cgtacgcctg tgtcatcgcc gtcggctgac gggcagccga cagtatcagc
     1381 ggcttccagg agggtccagc cgcccggggg gctatccccg agcgagtccg gcgcgccgcc
     1441 gccagccctc ggggagcagc agctcgatca catcatcgac cgcgacgacg ccgatcggtt
     1501 tctcgtcggt gtcgatcacc gggatcgcca gcaggttgaa gtcggccatc aatcgtgcga
     1561 cctcgggaag ctcggtctcc ggtgagaccg tcggcaccgc ggtttcgatg agctcagcga
     1621 cggtcgcgtg ctcggccgcg cggaccagct cggaggccag gacgccgccg gcgagatgac
     1681 cgtcagcatc gatcacccac aaggtcaaga gctggcccgg ctcaagctcg ctgccgcgca
     1741 ccacagaaag cgcctgcgcg accgtggcgt tggccggcaa cgacgcgaaa tccgggttca
     1801 tcagaccacc tgcagtcgac gggttatagc ccaacaaccg cttgatcttc agctgcttgg
     1861 cggcggacag gagattcagg accgggatcc ggcggtcctg ctcgatctcc agcagcaggt
     1921 cggcggcgtc gtcgctctcc atccgcccga gcaccgccgc gacctcctcg tcggagcggt
     1981 cacgcaggaa ctccacctgg tgttcgtcgt tg
//
LOCUS       DATCBJ010000215         2338 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_374379, whole genome shotgun sequence.
ACCESSION   DATCBJ010000215 DATCBJ010000000
VERSION     DATCBJ010000215.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2338)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2338)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2338
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_374379"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1115)
                     /locus_tag="VII50_04375"
     CDS             complement(<1..1115)
                     /locus_tag="VII50_04375"
                     /inference="COORDINATES: protein motif:HMM:NF024799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HEY5050113.1"
                     /translation="MRVTKGLQISAVVTASVLLAAACSSSKKTASTTPSSAAPAATTS
                     AAAAATSAPAAPTSAAAPATSAPAAPAAGGGLIGVDYPRSDSDFWNAYIRYVPQMATT
                     LGATIKTTASGNDITKLNANADSLVAEGAKALVLAPQDTAGIIPELAKLASKNIPVVT
                     IDTEPDSGKVFMIVRADNKAYGTKSCNYIGTQLKGVGNVVEFEGDLASVNGRDRSTAF
                     GACMTANFPKIKVFAEPTKWDTPTAIGQLNTVLAGNKIQGLYMQASIYLSATLADLSS
                     KGLLKPAGTAGHIVMVSNDGVKAEFTAIQAGSLDATIDQPADLYAKYGLYYAQQALAG
                     KTFSLGPTDHNSNIVTAADGDLEDQLPASLVTVDGAYP"
     gene            complement(1183..>2338)
                     /locus_tag="VII50_04380"
     CDS             complement(1183..>2338)
                     /locus_tag="VII50_04380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020661760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HEY5050114.1"
                     /translation="FTIGRGTEVEVAGIRAVEQLVVGLDIDEVFGDLGGFSQHLVGDS
                     PLRWLGPEKGVIHMAIGAVVNAVWDMYATREGKPLWKALADMSPQQLVDLVDFRYLTD
                     ALEPAEALDMLQRAREGAPGREQQLLADGFPAYSTSAGWLGYSDDKVARLCKEAVEAG
                     FDHLKLKVGGNIEDDIRRCELVRNLVGDDVRLSIDANQKWGVDEAIESVARLAHANLW
                     WVEEPTSPDDVLGHGQIARAIAPIKVAAGEHVANRVMFKQLLASGAIDICQIDATRVA
                     GVNENVAILLLAAKFGVPVCPPAGGVGLCEVVQHLAMFDFVALSGSTENRWIEYVDHL
                     HEHFVDPVRIDNGRYIAPRRPGSSARMHPESIAAYRYPDGRVWQLGHASS"
BASE COUNT          393 a          734 c          762 g          449 t
ORIGIN      
        1 gggtaggcgc cgtcgaccgt gaccagcgag gcgggaagct ggtcttcgag gtcgccgtct
       61 gccgcagtca cgatattgct gttgtgatct gttggcccta gcgagaacgt cttaccggca
      121 agagcctgct gcgcgtagta caggccgtac ttcgcataga ggtcggcagg ctggtcgatc
      181 gtggcgtcaa gcgagcccgc ctggatcgcg gtgaactccg ccttcacacc gtcattggag
      241 accatgacga tgtgcccagc ggtaccggcc ggcttcagca gacccttgct cgacaggtcg
      301 gcaagcgtcg ccgacaggta gatgctcgcc tgcatgtaga ggccctggat cttgttgcct
      361 gcgagcacgg tgttcagctg gccgatcgcg gtcggggtgt cccacttagt cggctcggcg
      421 aagaccttga tcttcgggaa gttcgccgtc atgcaggcac cgaaagcggt gctgcggtca
      481 cggccgttga cggacgcgag gtcgccctcg aactccacca cgttgccgac acccttgagc
      541 tgggtgccga tgtagttgca cgacttggtg ccgtaggcct tgttgtcggc acgcacgatc
      601 atgaagacct tgccactgtc gggctcggtg tcgatcgtga ccactggaat gttcttgctc
      661 gcgagcttcg ccaactcagg aatgatgccg gcggtgtctt gcggtgcgag cacgagcgcc
      721 ttcgcgccct cagcgaccaa cgagtcggcg ttggcgttga gcttcgtgat gtcgttgccc
      781 gacgcggtcg tcttgatcgt tgcgcccaga gtcgtcgcca tctgcgggac gtaccggatg
      841 taggcgttcc agaagtcaga gtccgaacgc gggtagtcga cgccgatcaa gcctccacca
      901 gcggcgggtg cggctggcgc tgacgtcgcc ggagcagcgg cggaggtcgg cgccgcaggg
      961 gcggaggtgg ccgcggctgc cgctgacgtg gtcgcggcag gtgcggcact gcttggagtc
     1021 gtgctggcag tcttcttcga gctgctgcat gcggcggcga gcagaacgga cgcggtcacg
     1081 accgcgctga tctgcagccc cttggttact ctcacttgct ctcctcttga gatgttcggg
     1141 ccgaacccgg gtcgtcgggc gcggtgaaac cgttgctgca gttcacgagc tggcgtggcc
     1201 gagttgccag acacgtccgt cgggatagcg atatgcggcg atcgattccg ggtgcatccg
     1261 ggcactactg cccggtcgtc gtggtgcgat gtagcggccg ttgtcgatcc gaacgggatc
     1321 gacgaagtgt tcgtgcaggt gatcgacgta ctcaatccat cggttttccg tcgagccgct
     1381 cagcgcaacg aagtcgaaca tcgcgagatg ctggacgact tcacagagac cgactccacc
     1441 cgcgggcggg cataccggca ccccgaattt ggctgccagc aagagaatcg ccacgttctc
     1501 gttcacgccg gcgactctgg tggcatcgat ctggcagata tcaatcgcac cgctggcgag
     1561 cagctgcttg aacatgacgc gattcgcgac atgctcacct gcggcgacct tgatgggcgc
     1621 aatcgcgcgc gcaatctgcc cgtgcccgag aacatcgtcg gggctcgtcg gctcttcgac
     1681 ccaccagaga ttcgcgtgcg ccaagcgagc gacactctcg atggcctcgt cgactcccca
     1741 tttctggtta gcgtcaatcg acagccggac gtcgtcaccg accagattgc gcaccaactc
     1801 gcaacgccgt atgtcgtctt cgatgttgcc gccgaccttg agcttgagat ggtcgaatcc
     1861 ggcttcgacg gcctccttgc acaaccgggc gaccttgtcg tcggagtaac cgagccaacc
     1921 cgcagacgtc gagtaagccg ggaatccgtc ggccagcaac tgctgctcgc ggccaggcgc
     1981 accctcgcga gcgcgttgca gcatgtcgag cgcttcagcc ggctcgagcg cgtcagtgag
     2041 gtagcggaag tcgacgaggt cgacaagttg ctgcgggctc atgtcggcaa gcgccttcca
     2101 cagcggcttg ccttcgcgcg ttgcgtacat gtcccacacc gcgttgacga cggcgccgat
     2161 cgccatgtgg atgacgccct tttcggggcc gagccagcgc agcgggctat caccgacgag
     2221 gtgctgcgaa aagccaccga gatcgccgaa cacttcatcg atgtcgaggc cgacgacgag
     2281 ttgctcgacc gcacggatcc ccgcgacttc cacttcggtg ccgcgcccga tcgtgaac
//
LOCUS       DATCBJ010000216         2277 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_530637, whole genome shotgun sequence.
ACCESSION   DATCBJ010000216 DATCBJ010000000
VERSION     DATCBJ010000216.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2277)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2277)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2277
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_530637"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..748
                     /locus_tag="VII50_04385"
     CDS             <1..748
                     /locus_tag="VII50_04385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5050115.1"
                     /translation="ENSFWDLGLIVGVPALLFFPDGVVPSPRWRATLRLYVVGGVLVV
                     ISQCVAATILVRFHDLQVDTNGQPVGTPETAGFAAFLQSGVAVLILLPLMVSWVVHLA
                     VAFRRSAGVLRQQLKWATVGALVLVVSLAVSVPLGNDTSAVAHVISGLCLALFGAFPL
                     SLGLGILKYRLYDIDRLISRTLSYTVVTGLLIGVYVGIVVLATDVLSFSSPVAVAAST
                     LAAAGGLARSAKRRSASSIAVSIAPATTRS"
     gene            complement(863..1879)
                     /gene="hpnH"
                     /locus_tag="VII50_04390"
     CDS             complement(863..1879)
                     /gene="hpnH"
                     /locus_tag="VII50_04390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009076658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosyl-hopene transferase HpnH"
                     /protein_id="HEY5050116.1"
                     /translation="MPIPMRQGARIGWYLFAQKKLHRREKFPLIVELEPLFACNLKCN
                     GCGKIQQPHDLLAQRMPVERALAAIEEAGAPMVSLAGGEPLMHPQIDVMVNELVKRKK
                     YVYLCTNAELVRKRWDKFNFKPSPYFAFTIHIDGLQERHDESVAKQGVFDEAIEAIKF
                     LKEKGFRVTTNSTFFNTDTPQNIIDVMNFLNDEVKVDQMMISPAYAYEKAPDQDHFLG
                     VTETRELFSKAFAGGNRKRWRLNHSPLFLDFLEGKIDFECTAWGIPSYSLLGWQKPCY
                     LMADGYVKTYKELIEKTDWSKYGRGRDERCNNCMAHCGYEPTAVLATMGSIKESLRAL
                     REQG"
     gene            complement(1879..>2277)
                     /locus_tag="VII50_04395"
     CDS             complement(1879..>2277)
                     /locus_tag="VII50_04395"
                     /inference="COORDINATES: protein motif:HMM:NF014456.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HEY5050117.1"
                     /translation="DVLRRRFPSIESSATDDICYATTNRQQAVRAIAAESDVMMVLGS
                     ANSSNSVRLVEVAERLGVRAHLVDDATDILPEWLDGASTIGITAGASAPPHLVDEVIE
                     TLRALGPVDVIQRAVADEDVTFSLPKGVAG"
BASE COUNT          364 a          725 c          716 g          472 t
ORIGIN      
        1 tgagaactcg ttttgggatc tcggattgat cgtcggcgtg cccgcgctgc tgttctttcc
       61 cgacggcgtc gtgccgtcac ctcgctggcg cgcgacgtta cgtctgtacg tggttggcgg
      121 cgtgcttgta gtgatctcgc agtgcgtcgc cgccaccatc ctggtgcgct ttcacgattt
      181 gcaggtggac acgaacggcc aaccggtggg cacgcccgag acggctggct tcgccgcgtt
      241 ccttcagtcg ggtgtggcgg tgctcatact gctcccgctg atggtgtcgt gggtggtcca
      301 cctcgcagtg gcctttcgcc ggagcgcggg cgtcttgcgg cagcaactca aatgggcgac
      361 tgtcggcgcg ctagtcctcg tagtgagcct cgcggtgtcg gttcccctgg gcaacgacac
      421 gtcggcggtg gcacacgtga ttagcggcct ctgcctcgct ttgtttggcg cgttcccgct
      481 aagcctgggg ctaggcatct tgaagtatcg gctctacgac atcgatcggc tgatcagccg
      541 cacgctgtcc tacacggttg tcaccggcct gcttatcggc gtctacgtcg ggatcgtggt
      601 gctcgcgacc gacgtcctgt cgttctcatc acccgtcgcc gttgcggcgt caacgctcgc
      661 cgcagccggg ggtttagccc ggtccgcaaa gcggcgcagc gcgtcatcga tcgccgtttc
      721 aatcgcgccg gctacgacgc ggagctgacg gtcgctgcgt tctcggcgcg actcagagaa
      781 gcagtcgacc tcgacaccgt gcgcaccgac ttgctcacgg tcatcagccg cgcggtggaa
      841 ccagcccacg ccgcgatctg gatcagccct gctcgcgcaa cgcgcgcagg ctctccttga
      901 tcgaccccat cgtcgcgagc acggcggtcg gctcgtagcc gcagtgcgcc atgcagttgt
      961 tgcaccgctc gtcgcggccg cgaccgtact tgctccagtc ggtcttctcg atgagctcct
     1021 tgtacgtctt cacgtacccg tcggccatca ggtagcacgg cttctgccag cccaacaacg
     1081 agtacgacgg gatgccccac gcggtgcact cgaagtcgat cttgccctcg aggaagtcga
     1141 ggaacagcgg cgagtggttg agacgccagc gcttgcggtt gccaccggcg aacgccttgg
     1201 agaacagctc acgcgtctcg gtcacgccaa ggaagtggtc ttggtcgggc gccttctcgt
     1261 aggcgtacgc gggcgagatc atcatctggt cgaccttgac ctcgtcgttc aggaaattca
     1321 tcacgtcgat gatgttctgc ggtgtgtcgg tgttgaagaa cgtcgagttc gtcgtgaccc
     1381 ggaatccctt ttccttcaag aacttgatcg cttcgatggc ctcgtcgaac acaccctgct
     1441 tcgcgaccga ctcgtcgtga cgttcctgga gaccgtcgat gtgaatcgtg aacgcgaagt
     1501 acggcgacgg cttgaagttg aacttgtccc agcgcttgcg cacgagctcg gcattggtgc
     1561 acaggtagac gtacttcttg cgcttcacga gctcgttgac catcacgtcg atctgcgggt
     1621 gcatcaacgg ctcaccaccg gccagtgaca ccatcggtgc gccggcttcc tcgatcgcgg
     1681 caagcgcccg ttcgaccggc atccgctggg ccagcaggtc gtgcggctgc tggatcttgc
     1741 cgcaaccgtt gcacttgagg ttgcaggcga acagcggctc gagctcgacg atcagcggga
     1801 acttctcgcg tcggtgcagc ttcttctgcg cgaacaggta ccagccgatg cgtgctcctt
     1861 ggcgcatggg aatgggcatc agcctgcaac acctttcggt agtgaaaacg tgacgtcttc
     1921 atctgcgacg gcacgctgga tgacatcgac ggggccgagc gcgcgaagcg tttcgatgac
     1981 ctcgtcgacc aaatgcggcg gagccgacgc tcccgcggta atgccgatcg tcgacgcgcc
     2041 atcaagccac tcgggcaaga tatcggtggc gtcgtcaacc agatgtgccc gtactcccag
     2101 gcgctcggct acttcgacca ggcgcacgga attcgaggag ttcgcggatc cgagcaccat
     2161 catgacgtcg gattcagcgg cgatcgcgcg caccgcctgt tgccggttgg tggtcgcata
     2221 gcagatgtcg tcggtcgcag atgactcgat cgacgggaac cgccggcgca agacgtc
//
LOCUS       DATCBJ010000217         3342 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_258139, whole genome shotgun sequence.
ACCESSION   DATCBJ010000217 DATCBJ010000000
VERSION     DATCBJ010000217.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 3342)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3342)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3342
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_258139"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..238)
                     /locus_tag="VII50_04400"
     CDS             complement(<1..238)
                     /locus_tag="VII50_04400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009078063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEY5050118.1"
                     /translation="MPRLTADNLQARRRTIIDGARRCFARDGYEGATVRRLEAEIGLS
                     RGAIFHHFGDKESLFFAVADDDAAEMAAVVAEYGL"
     gene            304..636
                     /locus_tag="VII50_04405"
     CDS             304..636
                     /locus_tag="VII50_04405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5050119.1"
                     /translation="MLAGIFGVTAVLFALAGCRQTASLSKQEIVVQFTTDATAADHAR
                     VWALCQHIDNVDPEPLVTKSKYPATLLYNVRFRVDHASNLELQQLFDCLKQDPTVSGY
                     NTGDSADN"
     gene            765..2348
                     /locus_tag="VII50_04410"
     CDS             765..2348
                     /locus_tag="VII50_04410"
                     /inference="COORDINATES: protein motif:HMM:NF019113.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HEY5050120.1"
                     /translation="MKHAAAVGAALAITLTGATAGISGPSALASTVTLPSYSAVVNAS
                     KLAANYYRTTYAHTTVTPTNGWSWSTYTKGVLALFEQAGDQHFLSDELAWGQSNAWGI
                     SNESDPPNRLTAAQTYDDLAALSSTVSLTAADSAMASNLTSLPVSQYYYADSLFVGLP
                     AWTRWATRTGNSAYLAGLDAMYAWARDEGGTSSLCKGKAVPSTGLFDATQGLWYRDCR
                     FDGATDTAGKPIFWARGNGWVIAAMAQVLATLPAGDPRAATYASMLQTMAARLAHLQG
                     SDGLWRSSLLDPSLYPSPETSGSALITYALAYGIKAGILNSATYLPVVAKAWHGLTTI
                     SLQPSGFVTDCQGPDSAPGAPYTANSPRINQSSTSSGTVNLDSPPYCVGALLLAGAQV
                     AQLITSPSTGRPAVATGQPVVGYGANRVNDGDVTTRWSVSGFPQSVTIDLGSNKSLSN
                     AKVVPYLDRAYRYRVQTSTDNKNWTTVINRTTNTLTGSRLDTFTTGPVNARYVRLTVV
                     GVYGVSTSWVSIQEFAVYP"
     gene            complement(2353..3333)
                     /gene="nadA"
                     /locus_tag="VII50_04415"
     CDS             complement(2353..3333)
                     /gene="nadA"
                     /locus_tag="VII50_04415"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HEY5050121.1"
                     /translation="MLPQQPPPDGWADEIRSLAAARNAVILAHNYMLPDIQDVADHVG
                     DSLALSRIAAASDADTIVFCGVHFMAETAKILAPNKTVLIPDLDAGCSLAASIDVADV
                     LAWKADHPDGVVVAYVNTSAAIKAEVDVCCTSSNAADVVRSIPEGKEIFFLPDQFLGA
                     HVRRVTGRDMDVWAGECHVHAEISPSDLRATLAANPEADLVVHPECGCTTTALYLTST
                     GDLPADRTRILSTGGMVEYAKTTTAPRVLVATETGILHQLRKLNATTDFMPVSPRAVC
                     PYMKMITPAKLLRSLRENVFEVDVDPAMAARARHAVERMIEIGSPSRGGE"
BASE COUNT          602 a         1100 c         1062 g          578 t
ORIGIN      
        1 ccaagccgta ttcggcaacc actgcggcca tctcggcggc gtcgtcatcg gccaccgcga
       61 aaaacagcga ctccttgtcg ccgaagtggt ggaagatcgc gcccctcgag agcccgatct
      121 cggcttcgag tcgccgcacg gtcgcgcctt cgtagccgtc gcgcgcgaaa cagcggcggg
      181 cgccgtcgat gatcgtgcga cggcgggcct ggaggttgtc cgcggtaagc cggggcacaa
      241 tcagcagagt accgaccgcg tcgatagcct ggctcgatgc gacatgctgc tggccgtcgg
      301 cgcgtgctcg cggggatctt cggggtcacc gccgtgctgt ttgcgctggc ggggtgccga
      361 caaacggcaa gtctgtcgaa acaggaaatt gtggtccagt tcacaaccga cgctactgcc
      421 gccgaccatg cgcgggtctg ggccctgtgc cagcacatcg acaacgtcga ccccgagccc
      481 ttagtcacga agagcaaata cccggcaacg ctgctctaca acgtgcgctt tcgcgtcgat
      541 catgcgagta atctagagtt gcagcagctc ttcgattgcc tgaagcagga tccgacagtg
      601 agtggataca acaccggcga cagcgccgac aactaggcag cgacgggcac gccatagccg
      661 caggcaccgc agcgccactc accacggtcg cgttcatcgc acccggtgcg cgcgtcgact
      721 cagggcatgg gcggagagca aggttttcct tgagagaagg gcacatgaag cacgcagccg
      781 cagtaggagc ggctctcgca atcacattga ccggcgcgac ggcggggatt tccgggccgt
      841 cggcattagc gtcgaccgtg acattgccga gctattcggc cgttgtcaac gcgtctaaac
      901 tcgcggcgaa ctactaccgg acgacgtacg cacacaccac ggtgacgccc acgaacggct
      961 ggtcgtggtc gacgtatacg aagggcgtgc ttgctctgtt cgagcaggcg ggggaccagc
     1021 atttcttaag cgacgagctg gcctggggcc agtccaacgc gtggggaatc tccaatgagt
     1081 ctgatccgcc caaccggctc actgcggcgc agacgtatga cgacctagcg gccctatcct
     1141 cgacggtctc gctgactgcc gccgattcgg cgatggccag caacctgacc agcctgccgg
     1201 tctcgcagta ctactacgct gattcgctct tcgtgggcct tccggcctgg acgcgttggg
     1261 caacccgcac cggcaactcg gcatacctgg ccgggctcga cgccatgtac gcatgggcgc
     1321 gcgacgaagg cggcacaagt tccctgtgca agggcaaagc cgtgccgagc accggtcttt
     1381 tcgatgcgac gcagggactc tggtaccgcg actgcagatt cgacggggcg actgacacgg
     1441 ctggcaagcc gatcttctgg gcgcgcggca acgggtgggt tattgccgcg atggcccagg
     1501 tgctcgcgac gctccccgcc ggtgacccac gcgccgcaac gtacgcgagc atgcttcaga
     1561 ccatggcggc gcggctcgcc caccttcagg gtagtgacgg tctgtggcgc tccagcctcc
     1621 tggacccgtc cctctatccg tcgcccgaga cgagcggcag cgcgctcatc acctatgcgc
     1681 tggcctacgg aatcaaggcg ggcattctca actcggcgac ctatctccct gttgtcgcca
     1741 aggcgtggca tggcttgacc acgatctctt tgcagccaag cggtttcgtg accgactgcc
     1801 aggggcccga tagcgctcct ggcgcgccct acacggctaa tagcccgcga atcaaccagt
     1861 cctcgacgtc atcgggcacg gtgaacctcg actcaccgcc ctattgcgtc ggcgcattgc
     1921 tgctggcagg tgcgcaggtc gcgcaactca tcaccagccc gtcgacgggc cgtccagccg
     1981 ttgctaccgg ccagccggtg gtgggttacg gggccaaccg cgtcaacgac ggcgacgtca
     2041 cgacgcggtg gtccgtgtcc ggcttcccgc agtcggtcac gatcgacctc ggctcgaaca
     2101 agtcgttgag caacgccaag gtcgtgccct atctcgatcg ggcataccgc taccgggtcc
     2161 agacatccac cgacaacaag aactggacga cagttatcaa ccgaacgacc aacacgctca
     2221 ccggatctcg gctcgacacc ttcacgacag gcccggtcaa tgcccgttac gtgcggctca
     2281 ccgtcgtcgg ggtgtacggc gtcagcacct cgtgggtcag tattcaggag tttgcggtct
     2341 acccctgacg cgtcactcgc caccgcgcga tggcgacccg atctcgatca tgcgttccac
     2401 ggcgtgccgc gcacgtgccg ccattgccgg atcgacgtcg acttcgaaga cgttttcccg
     2461 caacgatcgc agcagtttgg ccggcgtgat catcttcatg taggggcaca ccgcccgcgg
     2521 cgagacaggc atgaagtcgg tcgttgcatt gagtttgcgg agctggtgca agatgccggt
     2581 ttcggtcgcg accagcacgc gcggcgccgt cgtggttttt gcgtattcga ccatgccgcc
     2641 ggtcgacagt atccgggtgc ggtcggccgg aagatcgccg gtactcgtga ggtacaaggc
     2701 ggtggtcgtg cagccgcact cggggtgcac gaccagatcg gcctccggat tcgcggcgag
     2761 cgttgcgcgc aggtcggacg gcgaaatctc ggcgtgcaca tggcactcgc ccgcccacac
     2821 gtccatgtcg cgaccggtca ccctgcgcac atgcgcgcca aggaactgat cggggaggaa
     2881 gaagatctcc ttgccctccg gaattgaccg cacaacatcg gccgcgttgg acgacgtgca
     2941 gcacacgtcg acttcagcct tgatggcagc ggacgtgttg acgtaggcca ccacgacgcc
     3001 gtcgggatgg tcggccttcc acgcgaggac gtcggccacg tcaatgctgg ccgccaagga
     3061 acatccggcg tcgagatcgg ggattagcac ggttttgttc ggcgcgagga tcttcgccgt
     3121 ctccgccatg aaatgcacgc cgcagaaaac gatcgtgtcg gcatcgctcg cagcggcgat
     3181 ccgcgacagc gccaacgaat caccgacgtg gtcggcgaca tcttggatgt cgggcagcat
     3241 gtagttgtgc gcgaggatca ccgcgttgcg cgcggccgcc agcgatcgaa tctcgtctgc
     3301 ccagccgtcc gggggcggtt gctgcggcaa caccggtacg gg
//
LOCUS       DATCBJ010000218         3366 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_227440, whole genome shotgun sequence.
ACCESSION   DATCBJ010000218 DATCBJ010000000
VERSION     DATCBJ010000218.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 3366)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3366)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3366
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_227440"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(3..500)
                     /locus_tag="VII50_04420"
     CDS             complement(3..500)
                     /locus_tag="VII50_04420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5050122.1"
                     /translation="MIVEGTSPDKSSTFNDADASQFNVTKVLKAIPGVNPSGTIEIVF
                     APPNSGDFLPAGMYLLFLEYNAIGKDYSIASSSFRNMLTVGEFLINGAGATEKRCSTP
                     VSDGTPATATASANAAAASTPSLSASQLEGFAGQLSIGNAPGVAQSSAPPVAAHTSAS
                     VPASS"
     gene            1142..2227
                     /locus_tag="VII50_04425"
     CDS             1142..2227
                     /locus_tag="VII50_04425"
                     /inference="COORDINATES: protein motif:HMM:NF015411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HEY5050123.1"
                     /translation="MEADPTRVSVVGLGLLGSAIARAFFDSGYELTVWNRSPERTRAF
                     DGTAQVASSVAEACYASDVIVVCLIAPDACRSALRYPAAEQALSGKLIVQLTTTTPGD
                     ALDEAGWASRYGATYLGGAILASPATVGTDRAKAFYAGPQDVFDEHRLLLQVLAPNSV
                     YLGDEVGRAATMDHALLELSYGCLAVLYHCMALCEAGSVPLQDFRDQVAIFPKGFLEQ
                     RAGAIGSGSYPSGSATMHTFVSWAQQLLRTAETAGVDTSLPSTLLQGITKKRRTRSWQ
                     RRLPGSIRSIPATLSDNPTRDQRQCARSDQGLPVLWARRRTSGSNAMGTTSWNTMTPT
                     VSKMSGHGLAKPLASTPKARPRLTRSG"
     gene            2643..3098
                     /locus_tag="VII50_04430"
     CDS             2643..3098
                     /locus_tag="VII50_04430"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY5050124.1"
                     /translation="MHTASVIRLGTPADYPAVAAVFRRASLFNDGDRDNLLAHPQHLV
                     LQSDGLEQGRTYVAGKHGSVVGFASWVETAETVDLEDLFVEPTWMRRGVATALVACIV
                     DVLRSQGVSCLEVTANPHALGFYRRVGFRDIGTAMTQFGQAPRMALVIV"
BASE COUNT          615 a         1034 c         1101 g          616 t
ORIGIN      
        1 atctacgacg aagcgggaac tgacgcagat gtatgggctg caactggcgg tgcgctcgat
       61 tgcgccacac cgggcgcgtt gccgatgctc agttgaccgg cgaagccctc aagttgagac
      121 gctgatagcg atggtgtgct ggccgctgcg gcgttcgccg aggccgtcgc ggtcgctggg
      181 gtgccatcgg acacgggagt ggagcatctc ttctcggtcg cgccagcacc gttgatcagg
      241 aactcgccga ccgtcaacat gtttcggaaa ctgctgctcg caatcgagta gtccttccca
      301 atcgcgttgt actccaggaa cagcaagtac atcccggcgg gcaggaagtc gcctgagttc
      361 ggtggggcga agacgatctc gatcgtgccg gagggattga cgccggggat agccttcaga
      421 actttggtga cgttgaactg cgacgcatcc gcgtcgttga aggtggacga cttgtccggc
      481 gaggtgcctt cgacgatcac gccactctcc cttgccactc cggataggtc cgacgcagac
      541 aagacggacc cacacaccca cgcgagaggg ggtgccaacc cggcaagagg cgagctgggc
      601 gcaacaacgg ccgcgctgga ggaagccgtc gcgccgcgcg gcgagtcaga accgctcgcc
      661 gagcgtgcag cggagttcgc gcatccacaa agtcccgcaa cgacgaccag ggcggggatc
      721 aacgctggca cgactcggac agatctcgtc acggcgctga tcagattctc gttccgtctt
      781 ttgtgagtgc cttcatgatg ctcagacgaa acgacgctgg tgcgcgttcc accacagggg
      841 tcccgcggca gcggttgact cgcgcttccc atcgccaatc ccgcggcgca cgtacctcag
      901 gctcgggcat tacgtgattg gagtcctcgg ttcggataga tccgctcatc ggcacgatcg
      961 aacgtgcgtc agagcggttg agggtgtatc gatcctggtt gctcactgat cgaactcggt
     1021 gatccacgat gtcgaacccc cgacgacccc gacgctactt ctcggccgcg ccctggatcg
     1081 ttcgcagcgt caggttgcat caggttctcg tcgttgacgt cgcggtgcat aggatctgag
     1141 ggtggaagcg gatcccactc gagtgagtgt ggttggactt gggctgttgg gctcggcgat
     1201 tgcccgagcg ttcttcgaca gcggctacga gctcacggtg tggaaccgtt cgccggaacg
     1261 gacgcgggcc ttcgatggga ctgcccaggt cgcgtcgtcg gtggccgagg cgtgctacgc
     1321 aagcgacgtg attgtggtgt gcctgatcgc tccagatgcg tgcagatcgg cgctccgata
     1381 cccggcggcg gagcaggcac tctccggcaa gctgatagtc cagctgacca cgaccacccc
     1441 cggtgacgcc ctcgacgagg ctggatgggc cagccgttac ggtgccacgt atctgggcgg
     1501 tgccatcttg gcttcgcctg cgaccgtggg aacagaccgg gcaaaggcgt tctacgccgg
     1561 gccgcaggac gtgttcgacg agcaccgctt gcttctgcag gttctggccc ccaactcggt
     1621 gtacctgggt gatgaggtcg ggcgggcggc gaccatggat cacgccctgc tcgaattgtc
     1681 ctacggatgc ctcgcggtgc tctaccactg catggcgttg tgtgaagccg ggtcagttcc
     1741 cctccaagac ttccgggacc aggtcgccat cttccccaaa ggattcctcg aacagcgggc
     1801 aggagcgatc gggtcaggta gctatccttc ggggagcgcg acgatgcaca cgttcgtctc
     1861 ctgggcccag caactgctgc gaacagccga gaccgccggc gtcgacacgt cgctgccctc
     1921 cacgttgctc caaggcatca ccaagaagcg tcgaactcgg tcatggcagc gacgactacc
     1981 aggctctata cgaagcattc cggcgacgct aagcgacaac cccacgcgcg atcagcggca
     2041 atgtgcgcgt tcagatcagg gattgcccgt gctttgggcg cgccgtcgaa ccagcggtag
     2101 caatgccatg ggcaccacca gttggaacac gatgacaccg acggtcagca agatgtctgg
     2161 ccacggactg gccaagccgc tcgcgagtac accgaaagca aggccgaggt tgactaggag
     2221 tgggtagacg gcgcgtttca tctgggtaat gcgcggttga gcctgggccg tttgctcgac
     2281 ggtggcgtgc accagcgcgg ggagaagcct cagcagcagt gcatagatcg ttgcggatac
     2341 cagcccgatg agcgcgcccc aggagaatag ccaccagcgc cagtggccga actggatggc
     2401 agcgacggcg catgccacga gaaccgccgc cccgctggcg tacatcagaa gacgagatcg
     2461 tggaccgtag ggatcgccga ggacgccagt tgcgcggcgg gccatgtggg cgtccgtcat
     2521 ggcgaggctt taccaccaaa gatgatcaaa caaacgcgca ccgcgaatcg cactctccct
     2581 gcggccacgt gaatgcgtgt cacagcggct cgagcgggcg tgtagcggat gcgcgccggt
     2641 cggtgcacac tgcgtcggtg atcagattgg gaacgcccgc tgactatccg gctgtcgcgg
     2701 cggtgtttcg tcgcgcatcg ttgttcaacg acggtgatcg agacaatctc ctggcccatc
     2761 cgcaacacct ggtccttcag tctgacggac tcgagcaagg ccgcacctac gtcgcgggga
     2821 agcacggatc agtcgtcggc ttcgcgtcat gggtcgaaac agcggagacg gtcgatctcg
     2881 aagacctgtt cgtcgaaccg acctggatga ggcggggggt agcgactgcg ctcgtcgcgt
     2941 gcatcgtcga cgttctgcgt tcgcaaggcg tttcgtgtct ggaggtgacc gccaacccgc
     3001 acgcgctcgg cttctaccga cgcgttggct tccgcgacat cggcaccgcc atgacacagt
     3061 tcggccaagc tcctcgcatg gcgctggtta tcgtctgatt cgactgatct gctcaaccca
     3121 gaatccaccg cgcagctcga tggccgtagg cagtcatctt cagccacgag gaccaagaac
     3181 tcgccggccc tggacgttgc acatcggtac ggtcgaacgc gcgtcatcgc ggctacttat
     3241 agcgcatgtt ggagctcggc cggcgcggct actcctgacg tcgccggctg tcatggtgac
     3301 cgcgtcggtg acgtagtcaa gggtgtgatc gctgctgagg tttggatgtg gcgtcctgca
     3361 ggtagg
//
LOCUS       DATCBJ010000219         2056 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_153134, whole genome shotgun sequence.
ACCESSION   DATCBJ010000219 DATCBJ010000000
VERSION     DATCBJ010000219.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2056)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2056)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2056
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_153134"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(154..>2056)
                     /locus_tag="VII50_04435"
     CDS             complement(154..>2056)
                     /locus_tag="VII50_04435"
                     /inference="COORDINATES: protein motif:HMM:NF016149.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="copper resistance protein CopC"
                     /protein_id="HEY5050125.1"
                     /translation="PASVLLYFSEQIDPSLSEATVSGPGGERVTAPPASARLIQVDLD
                     TNLPGSYRVTWRAVSDVDGHSTSGAFSFTVGQPAGATVANATASVSVTNWAVTVARWI
                     EDLALIVGVGSLFITWLGRRTDALPWVQPELTLVFGVALAAGVVVVAGEAVAAAGISV
                     HGLTAYFTTGLAGTARLVRILLEVAACVAAIRRVYAVLGVILVALVVALATSGHEASS
                     VGAVALDAGHVLSAGVWVGGILAMATLRPPDGWRAGGRALLARFTPWALATFVATIAL
                     GVVQAILNVGTWSALVSTTYGRVLIAKSAAVLLLIPLSLVAWRRRSPHLRIEAAISAT
                     VVLAASLLSAFPLPVDSGTASNSAVLTSDVGLPHGSDLTIGAQAGQTLVGVTIDPGAP
                     GRNSVTVYVLSADGAAASQSLPVTAVVDGRAMPLQACGDTCRKGTAQLAGSDALSIHV
                     GGAEGGTATVQLPALPARNGTALLDAALARMKDLTSVTMHETLTGGTGLPTDVTDYEE
                     VAPDRLMWTEPGGAAAISVGSLFSARERSGAPFVAQSGHDPVPEPNFSWQYFPAATAV
                     HLMGSALLDGVQTSVVEFFTGEPGTPVWFRFYVDGTDHVVLSDMSAPGHFMTQSFAHF
                     DAPLVINAP"
BASE COUNT          383 a          701 c          680 g          292 t
ORIGIN      
        1 ctccagcacc gccgaccgcg gccatcgatc ccacaaccag cacacgacga aaagtcccag
       61 aactcatatc aagtttcccc tgcctctcac gcccttctca aggattcgag cgtacctgcc
      121 gggggcttcc acgtcaagga ccacgcggtc ccgctagggc gcgttgatca ccagcggggc
      181 atcgaaatgc gcgaaggact gggtcatgaa gtggccgggt gccgacatgt ccgagagaac
      241 cacgtgatcc gtgccatcga cgtagaaccg aaaccacacc ggcgtacccg gctcacccgt
      301 gaagaattcg accacgctgg tctgaacccc atcgaggagc gcagaaccca tgaggtgcac
      361 ggctgttgcc gccggaaagt actgccatga gaagttcggc tctgggactg gatcatggcc
      421 ggactgcgcg acgaacggag cacccgaacg ttcgcgtgcg gagaacaagc tgccgacgga
      481 gattgccgcg gcgccgccag gctcggtcca catcaaccga tcgggtgcca cctcctcgta
      541 gtcggtcacg tcggttggaa gtcccgtccc gccggtgagc gtctcgtgca tggtcacgct
      601 ggtcaggtcc ttcatgcggg cgagggctgc atcgagcagc gcggttccgt tgcgcgcagg
      661 cagcgccggc agctgcaccg ttgcggtgcc accctcagct ccaccgacgt ggatcgagag
      721 cgcgtcgctc cccgcgagct gcgcagtgcc cttgcgacag gtgtcgccgc atgcctgcag
      781 cggcatcgcc cggccgtcca caaccgcggt tacagggagc gactgcgacg cggctgcgcc
      841 gtcagccgag agaacgtaca cggtcacgct gttgcggccg ggcgctcccg gatcgatggt
      901 gacgcccaca agcgtctggc cggcctgcgc accgatcgtc aaatcggagc cgtggggcaa
      961 gcccacatcg gacgtcagca cggcgctgtt cgacgctgtg ccgctgtcga ccggcagtgg
     1021 aaacgctgac agcaacgacg cggcgagcac caccgtcgca ctgatcgccg cttcgatgcg
     1081 caggtgcggt gatcgccgtc gccaggcgac gagcgacagc gggatcagga gcaggacggc
     1141 ggccgatttg gcgatgagca cgcgtccata ggttgtcgac accagtgccg accaggtccc
     1201 gacattgagg atcgcctgga cgacaccaag agcgatcgtg gcgacgaatg tcgcgagcgc
     1261 ccagggtgtg aagcgtgcca gcagggctcg tcccccggca cgccagccat ccggcgggcg
     1321 gagcgttgcc atggcgagga tgccgcccac ccacacgccg gcagagagca cgtggcccgc
     1381 gtcgagggca accgccccca ccgacgaggc ctcgtgacct gaggtggcaa gcgccacgac
     1441 aagggcaacg aggatcacgc cgagcactgc gtacacccgc ctgatagcgg ccacgcaggc
     1501 ggccacctcg agcagaatcc tgacgagcct cgcagttcct gccaatcctg tcgtgaaata
     1561 cgcggtcagc ccgtggacgc tgatcccggc ggcggcgaca gcttcgccgg cgacgacgac
     1621 gacgccggct gcaagcgcaa cgccgaagac gagcgtcaat tccggctgca cccatggcag
     1681 tgcatccgtg cggcgtccga gccaggtgat gaacagcgat cccacaccga cgatgagcgc
     1741 caggtcctcg atccagcgcg cgacggtgac ggcccagttg gtgaccgaga cagaggcggt
     1801 cgcgttcgcg accgtggcgc cggccggttg gcccacggtg aagctgaacg ccccacttgt
     1861 gctgtggccg tcgacgtcac tgaccgctcg ccacgtgacc cgatagctgc ccggcaggtt
     1921 ggtgtcgagg tcgacctgga tcagacgggc tgacgccgga ggagctgtca ccctttcgcc
     1981 gccgggcccg ctgaccgttg cctccgaaag gctcgggtcg atctgctcgc tgaagtacag
     2041 gagcaccgag gcaggg
//
LOCUS       DATCBJ010000220         2976 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_31321, whole genome shotgun sequence.
ACCESSION   DATCBJ010000220 DATCBJ010000000
VERSION     DATCBJ010000220.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2976)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2976)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2976
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_31321"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..233
                     /locus_tag="VII50_04440"
     CDS             <1..233
                     /locus_tag="VII50_04440"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEY5050126.1"
                     /translation="GVQLIALEGSAHEVLSRSAQNADVLVVGARGHSGPIALMLGSTA
                     QACTRHAHCPVLVVPSPVAVVPDPAHDDAPLD"
     gene            297..515
                     /locus_tag="VII50_04445"
     CDS             297..515
                     /locus_tag="VII50_04445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5050127.1"
                     /translation="MLAGVRSRDGCHENIANEVPRRHLVETFGGLLEEDDGEVSQEHL
                     RYGDTTKLTARDLSRRLVGDHTHQFHLS"
     gene            complement(808..1272)
                     /locus_tag="VII50_04450"
     CDS             complement(808..1272)
                     /locus_tag="VII50_04450"
                     /inference="COORDINATES: protein motif:HMM:TIGR00072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation protease"
                     /protein_id="HEY5050128.1"
                     /translation="MTLRRPLVIAYGNRLRGDDAVGWVVADALRDDVRMDLVDVVAVH
                     QLTPDLATEITTASRVVFVDARLDPAFAPGVVAVDTVLPEPDAGPMTHDIGAGTILSL
                     AEALYGVHPPAVAVTVAIKSVEPGADLSGAVGASVSQLVETVIRLCNETPDA"
     gene            complement(1269..2687)
                     /locus_tag="VII50_04455"
     CDS             complement(1269..2687)
                     /locus_tag="VII50_04455"
                     /inference="COORDINATES: protein motif:HMM:NF012592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe hydrogenase subunit alpha"
                     /protein_id="HEY5050129.1"
                     /translation="MASEITIDPVTRIEGHARIKISLDDAGRVGGAQFQVTQFRGFEK
                     ICQGRPLAEMPSLMARICGICPVSHLIASAKACDRILAVRPPKVGADLRALMNLAQIV
                     QSNALSLFHLASPDLLFGFDADPATRSIVGVAKAHPQLAMDGIYLRKFGQHAIELLGG
                     KRIHPAWIVPGGVESALTAEKRDEIRAMIPEAYAAADRALAWYKNNLLSWAEEAETFG
                     SFQSAFMALVDPDGNVAHYDGRLRVVDHDGTLLADFDAYDYADYIGEYTDEASFLKPT
                     YFKPRGIENGLYRVGTLARAIVAERFGTPRADAELSEFRSRFGRYPSSSFHYHQTRLL
                     DILYGIERMEMFLDDPDILATRVASHAEVNRNVGIGVAEAPRGTLIHHYVVDDDGIIQ
                     TANLIIATGHNAAAMNASVLQVARHFVKGERIEEGMLNRVEAVIRCYDPCLSCSTHAM
                     GQMPLHISLVGYDGEVLDEVRR"
     gene            complement(2715..>2976)
                     /locus_tag="VII50_04460"
     CDS             complement(2715..>2976)
                     /locus_tag="VII50_04460"
                     /inference="COORDINATES: protein motif:HMM:NF013242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5050130.1"
                     /translation="PGMRNVFADAQPLFQRAYIENVTVNLGMPSRGLPQHLPKTLPVH
                     KVVKVDVFLPGCPPSADLIFDTLVALLDGRVPEATTHARFGR"
BASE COUNT          574 a          953 c          910 g          539 t
ORIGIN      
        1 acggagtgca gctgatcgca ctggagggtt ctgcccacga ggtgctgagc cgcagcgcgc
       61 agaacgcgga cgttctggtc gtgggcgcac gtggtcactc gggtcccatt gcgctgatgc
      121 tcggctcgac cgcacaggca tgcacgcggc acgcacattg ccctgttctc gttgtgccca
      181 gccccgtcgc cgtcgtgccc gacccggctc acgacgacgc accgctggac taatagccgc
      241 tgcaccggcc aacgctccgt catcccgtag gccggtgcag ctccgctctc gacgtcatgc
      301 tggccggtgt tcgctcgcgg gatggttgcc acgagaacat cgccaatgag gtcccgcgcc
      361 gccatctcgt cgagaccttc ggtgggctcc tcgaagagga cgacggggag gtctcccagg
      421 agcacctgcg ctacggcgat acgacgaagc tgaccgccag agacctcagt cgcaggctag
      481 tcggtgacca cacccaccag ttccatctca gctaggcctg tgtcaccggt aatgtcgccg
      541 catcgaggac agaccctgat gtggccagcg aggtggtcgt gatccagcgg gccagcgaac
      601 ccgcacgcag cacagttcag agttatgtcg aagggttcac acacgagctg ggcagccgcg
      661 agcgaacggc ccgtcgctag catcatgaac gcctgacgga gcgtctcctc gctgatcgtt
      721 gttgcatgcc gcaccaccac cagcgcgaca cttcgcccgg ctgctctatg ctcgacttcg
      781 tcgatgagct cggcgacgag cgatagctca tgcatctgga gtctcattgc aaagccgaat
      841 caccgtctca acaagttgcg agaccgacgc gccaaccgcc ccagacagat ctgccccggg
      901 ctcgaccgac ttgatggcga ccgtgaccgc gaccgcgggt ggatggacgc cgtacaaggc
      961 ctcggcgagc gacaggatcg tgccggcgcc gatgtcatgt gtcatcggtc cagcgtcagg
     1021 ttctggcaga acggtgtcga ctgccaccac ccctggggca aacgccgggt cgagccgcgc
     1081 atcgacaaat accacacgcg acgcggtcgt gatctcagtg gcaaggtcgg gtgtcagctg
     1141 gtggacggcg acgacgtcga ccaagtccat ccgcacgtcg tctcgcagcg cgtctgcgac
     1201 gacccaccca acagcatcgt ctccgcgcag tcgattgccg tacgcgataa cgagcggacg
     1261 tcgcagcgtc atcgacgcac ctcgtcgagc acttcgccgt cgtagcccac cagagaaata
     1321 tgcaaaggca tctgccccat cgcatgagtt gagcacgaca ggcacgggtc gtagcaacgg
     1381 attacggcct cgacacggtt caacatgcct tcctcgatcc gctcaccctt cacgaaatgc
     1441 cgtgcgacct gcaggacgct ggcattcatg gcggccgcat tgtggccggt ggcaatgatg
     1501 aggttggcag tctgaatgat cccgtcgtca tcgacgacgt agtggtggat caacgttcca
     1561 cggggtgcct cagcaacccc gatgccgacg ttgcggttga cctctgcgtg tgaggctacg
     1621 cgcgtcgcaa ggatgtcggg atcgtcgagg aacatctcca tccgctcgat tccgtacagg
     1681 atgtcgagca acctcgtctg gtggtagtgg aaggagctgc ttggatagcg cccaaatcgg
     1741 gatcggaact ctgacagctc ggcgtccgcc cgcggtgttc cgaatcgctc ggcgacgatc
     1801 gcccgcgcca gcgttccgac tcggtacaag ccattctcga tcccgcgtgg cttgaaatac
     1861 gtgggcttga ggaaggacgc ctcgtcggtg tactcgccta tgtagtcggc gtagtcgtac
     1921 gcatcgaagt cggcgagaag agtgccgtcg tgatcgacaa cgcgcaaccg cccatcgtaa
     1981 tgcgcgacgt tgccgtctgg gtcgaccaga gccatgaacg ccgactgaaa ggaaccaaaa
     2041 gtctccgctt cctcggccca gctcagcagg ttgttcttgt accaggccaa ggcgcgatcc
     2101 gctgccgcgt aggcctcggg aatcattgcg cggatctcgt cgcgcttctc ggcagtcaac
     2161 gccgactcga cgccgcccgg cacgatccag gccggatgaa tccgcttccc accgagcagt
     2221 tcgatggcgt gctggccgaa cttacgcaga tagatgccgt ccatcgccaa ctgaggatgc
     2281 gccttagcta cgccgacaat gctgcgcgtg gcgggatcag catcgaagcc gaacagcagg
     2341 tccggcgagg cgaggtggaa cagactcaag gcattcgact gcacgatctg agcaaggttc
     2401 atcagagctc gcaggtccgc accaaccttg ggaggcctga ctgcaagaat gcggtcgcag
     2461 gctttcgcgg aagcgatcag atggctgacg ggacagattc cgcagattct cgccatcagc
     2521 gagggcattt ccgccaaggg tcgcccttgg catatcttct cgaagccgcg gaactgcgtg
     2581 acctgaaact gtgcgccccc gacccgtccc gcatcatcga ggctgatctt gatccgtgcg
     2641 tgaccttcga tcctggtgac cggatcgatg gtgatctcgc ttgccatcag gctcctactc
     2701 ggttgctgga cacgtcatct gccgaaccgc gcatgagtgg tggcttccgg tacgcgcccg
     2761 tcgagcagcg cgacgagggt gtcaaatatc aaatcggcgg acggcgggca gcccggcagg
     2821 aacacatcga ctttcaccac tttgtgcacc ggcaacgtct tcggcagatg ttgtggcaag
     2881 cctcttgacg gcatgcccaa gttgacggtg acgttctcga tgtatgcccg ctgaaacaaa
     2941 ggttgggcat cagcgaagac gttgcgcata cccggc
//
LOCUS       DATCBJ010000221         5646 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_232647, whole genome shotgun sequence.
ACCESSION   DATCBJ010000221 DATCBJ010000000
VERSION     DATCBJ010000221.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 5646)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5646)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5646
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_232647"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     regulatory      196..331
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            322..1680
                     /locus_tag="VII50_04465"
     CDS             322..1680
                     /locus_tag="VII50_04465"
                     /inference="COORDINATES: protein motif:HMM:NF014218.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine permease"
                     /protein_id="HEY5050131.1"
                     /translation="MTTTVPAHAEAPITLDQPAAKALGFFDQVGLWGNLGVSLLGFTA
                     ALFVLQPRAGSPGLPLTAALLATFVGTALGAAAVGLAAIPGARTGSPAMVLLRGLFGA
                     RLSYLPTGLNILQLLGWATFEIVIIASAAHQLWPSVPQNGYVLIAGVMTTALALRPLG
                     VVRVLRKYVTVAVAIVVVYLFVQLLRRPLPAVAHSSWREFWPAVDTALAVAISWVPVA
                     ADYSRHSKSGRTAFSAAFVGYTITQTACYALGIVAILSVPAASDGSNHGVWGAFIAVF
                     LGKAAFAVLAIREVDQSFCDVYSTAVSTQNLRPHWDRRILAFVVGTAATLLALLFHIG
                     NYQNFLYLIGSVFVPLFGVLIVDYFALQGHRRWDLAEHAPSRWLMLLPWIAGFCVYQL
                     INPGVIPRWAGFWGRVARDIHFTPQTWMSASLLSFVAAAVLTVAVRLADRRRLHEVRS
                     AP"
     gene            complement(1683..2003)
                     /locus_tag="VII50_04470"
     CDS             complement(1683..2003)
                     /locus_tag="VII50_04470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjQ family protein"
                     /protein_id="HEY5050132.1"
                     /translation="MLIVTTNDLPGYRVDEVLGEIFGLTVRSRNAFSQMGAGLKSILG
                     GELRGMTTALTDSRNQVMQRLIDEATARGANAILAMRFDTSEMGGVWTEICAYGTAVR
                     VTKL"
     gene            2076..3920
                     /locus_tag="VII50_04475"
     CDS             2076..3920
                     /locus_tag="VII50_04475"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY5050133.1"
                     /translation="MSDNLLAARALDAMVDAVVAGDRDGGVRLWNLGAERLLGWSAKS
                     VVGGQFPGIPDEHARQERDAALARVTAGEDVSLATTQRKADGTVVDVWIVFSPMRAWL
                     DGPIDGWLAVIRDATQQRAVQRGLRRRVELVGRLASVIASLNSDLDLHTVLQRISESG
                     RELLAADGAAYVMLDGDDLVIAAVSGLESQLVGERIPLAESAVATVLESGRTSIALNN
                     DDYPNSSTLVAATSARLPRLAIGLTRLDGLPSGALYVFFVAQGRALGKAELSVLELLA
                     EAAGAALANARAYDLTRRQREHERAVIEATLDGMAVVDADGLVRQWNPAAHELTGLSQ
                     AAALGHPLPFPAPDPGVVSEHQLASGRWLEVLCAQIADTGETVVDFRDVSRSKSIEES
                     KDLFLAVTSHELRTPITVVQGYASTLLTHWENITDEERRGSVERIAERTRALAALVEQ
                     LLLGSRAGLAAPMPSHVMFDLGGLLRTAVAGFKNVAQGHEFVLDVAPDVPLAVGDPSS
                     IEIVFSQLLENAIKYSPDGGAITVSVRTEGETIIAQVCDRGLGIPTGEHGQVFERFYQ
                     VGGERRRFGGVGLGLYIVQRLLEAHGGAVRAQPRDGGGTCFEVTLPGG"
     gene            3996..4451
                     /locus_tag="VII50_04480"
     CDS             3996..4451
                     /locus_tag="VII50_04480"
                     /inference="COORDINATES: protein motif:HMM:NF024970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY5050134.1"
                     /translation="MSSLRVTANPASVGYVRAVLRDDLAVLPEPVREEVALIATELLG
                     NAVLHARALDDGQLSIAWGVGEYGVEIAVTDGGAPTHPTVAEPGSEDTSGRGLSIVAT
                     LAARWGVEQHDSSTTVWAVIPLTEREWIDRELNSRESISLGSKVAARAL"
     gene            4546..5484
                     /locus_tag="VII50_04485"
     CDS             4546..5484
                     /locus_tag="VII50_04485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009077337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucuronic acid decarboxylase family
                     protein"
                     /protein_id="HEY5050135.1"
                     /translation="MITGGAGFLGSHLCERLLRDEYEVLCLDNFLTGTPNNVEHLLGN
                     DRFRLLKVDVTDWIHISGPVDAVLHFASPASPADYLQLPIQTLKVGSVGTFHAVGLAQ
                     EKGARFLLASTSEVYGDPQVHPQPETYWGHVNPIGPRGVYDEAKRFAEAMTVAYRDHR
                     EVDAAIVRIFNTFGPRMRPDDGRAIPTFISQALRGEPITVAGDGSQTRSVCYVDDLVN
                     GIVRMLQSDLPGPVNLGNPHELSVLELAELIRDLAGSSSSIEFVALPQDDPLVRRPDI
                     TVATRDLGWSPQVDVEDGLRRTIDWFRTHPDLVGIS"
     gene            5494..>5646
                     /locus_tag="VII50_04490"
     CDS             5494..>5646
                     /locus_tag="VII50_04490"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEY5050136.1"
                     /translation="MALLTEAVVVISGAGRGIGRGEAIELARDGARVVVNDVDAEAAS
                     AVVADIN"
BASE COUNT          838 a         1878 c         1913 g         1017 t
ORIGIN      
        1 cgaggtcgaa gccgagttga agcggctcgg cggtgacgac cgaccaggcg tctgcaaccg
       61 tcgtccccgc ccgcaaaccg tcaagcaggt tgtacaaggc gtgctccgca ccgccgactc
      121 tgccggcccg cacgaaacat gcgctgaccg cagtagccat ggtcatcgac cctactgccg
      181 gcgcgtacgc tggcagccgc acaccgcggg agctcgggcg aaaccgggct gagagggcgg
      241 ctggccggga cctcgacatg aaccggacgg gccgccgacc gctcgaacct gaccgggtaa
      301 tgccggcgta gggagtggca gatgacgacg accgtgccgg cacacgccga ggcaccgatc
      361 accctcgacc agccagccgc caaggcgctc ggcttcttcg atcaggttgg gctgtgggga
      421 aacctcgggg tcagcttgct cggtttcacc gccgcgctgt ttgtgctgca accgagggcc
      481 ggcagtccgg gcctgccgct gacggcggcg ctgctcgcca cgttcgtcgg cacggcgctc
      541 ggtgctgccg cggtcggtct cgcggcgatc cccggcgcgc gaaccggatc gccggcgatg
      601 gtcttgctgc gtgggctttt cggcgctcgc ctgtcgtacc tgccgaccgg tctgaatatc
      661 ttgcagctgc tgggctgggc caccttcgag atcgtgatta tcgcgtcggc cgcgcaccag
      721 ctctggccat cggtacccca aaacggttac gtcctcatcg ccggtgtcat gacgaccgcg
      781 ctcgcccttc gcccgctggg cgtcgtccgc gtgcttcgca agtacgtgac cgtcgcggtc
      841 gcaattgtcg tcgtctacct gttcgtccag ctactgcggc gtccgcttcc ggcagtcgcg
      901 cacagctcgt ggcgcgagtt ctggcccgcg gtcgacacgg cacttgcggt cgccatctcg
      961 tgggtgccag tcgcggccga ctactcacgc cattcaaaat caggccggac ggcgttcagt
     1021 gccgcgttcg tcggctacac gatcacccag acggcgtgct acgcgctcgg catcgtggcg
     1081 atcctgagtg tgccggccgc cagcgacggc tccaaccatg gcgtctgggg ggcgttcatc
     1141 gcggtgttcc tgggcaaggc ggcgttcgcg gtgctcgcga tccgcgaggt cgatcagtcc
     1201 ttctgcgatg tctattcgac cgcggtcagc acccagaatc tgcggccaca ctgggatcgg
     1261 cgcatcctcg cgttcgttgt cggcactgcc gcaaccctgc tcgcgctgct gttccacatc
     1321 ggcaactatc agaacttctt gtatctcatc ggctcggtct tcgtgccgct gttcggcgtc
     1381 ctgatcgtcg attacttcgc gctgcaaggg caccggcgat gggacctcgc cgagcacgcg
     1441 ccgtccagat ggttgatgtt gttgccatgg atcgcgggct tctgcgtcta ccagctgatt
     1501 aaccctggtg tgatcccacg ctgggcaggg ttctggggcc gcgtcgcgcg cgacatccac
     1561 ttcacgccgc aaacctggat gagcgcgtca ttgctgtcgt tcgtagccgc cgcagtgctg
     1621 acggtcgcgg tgcggctcgc cgatcgtcgt cgtctgcatg aggtgcgttc cgcgccctag
     1681 ccctacagct tggtgaccct gacggcagtg ccgtaggcgc agatctcggt ccagaccccg
     1741 cccatctccg aggtgtcgaa ccgcatcgcg aggatcgcgt tcgcgccgcg tgccgtcgcc
     1801 tcgtcgatga gccgctgcat cacctggttc cggctgtcgg tgagggcggt ggtcatgccg
     1861 cgcagctccc cgccaaggat ggatttcagc ccggcaccca tctggctaaa ggcgttacgt
     1921 gaccgcacgg tgagtccgaa gatctcaccg aggacctcgt cgacgcggta cccgggcagg
     1981 tcgttcgtgg tgacgatcag catgtcgacc accctagggc acgttcaccg ggacagtcgc
     2041 ggcttaaggg gcgccggcgg tgacacgatg acgccatgtc cgacaaccta ctcgcggccc
     2101 gcgccttgga cgcgatggtc gatgccgtcg tggcgggcga tcgcgacggg ggggttcggt
     2161 tgtggaacct cggagcggag cggctcctcg ggtggagcgc gaagagcgtg gtcggtggcc
     2221 agtttcccgg catccccgat gagcatgccc ggcaggagcg cgatgccgcg ctggcgaggg
     2281 tgacagccgg cgaggacgtg tcactcgcga cgacccagcg gaaagccgat ggcaccgtcg
     2341 tcgacgtgtg gatcgtcttc agtccgatgc gggcgtggct cgacggtccg atagacgggt
     2401 ggctcgccgt catccgcgat gcaacgcagc agcgggcggt gcagcgcgga ctgcggcgcc
     2461 gcgtcgagtt ggtgggccgg ctcgcgtctg tcatcgcgag cctgaactcc gacctggacc
     2521 tgcacaccgt gctgcagcgg atcagcgagt caggtcgtga gctgctggcc gccgacggcg
     2581 ctgcctacgt catgctcgac ggcgacgatc tggtgatcgc ggccgtctcc gggctcgagt
     2641 cgcagctggt cggtgagcgc attccacttg ccgagtcggc cgttgcgacc gtgctggagt
     2701 cggggcgtac gtcgatcgcc ctgaacaacg acgactaccc caacagctcg acactcgttg
     2761 ctgccacgag tgcgcggctg ccacggctcg cgatcggcct cacccgcctc gacgggttgc
     2821 catcgggagc gctctacgtg tttttcgtgg cgcaaggccg tgccctgggc aaggctgagc
     2881 tcagcgtgct cgagctgctc gccgaagctg ccggcgcggc tctcgcgaac gcacgcgcct
     2941 acgacctgac ccgacgacaa cgcgagcacg aacgcgccgt catcgaggcg acgctcgacg
     3001 ggatggcggt ggtcgacgcg gacgggcttg tgcggcagtg gaatcccgcc gcacacgagc
     3061 tgaccgggct gtcacaagcg gcggcgcttg ggcatccgtt gcccttcccc gcgccagatc
     3121 ccggggtcgt ctccgaacac cagcttgcat caggccgctg gctcgaggtg ctctgtgcgc
     3181 agatcgccga taccggtgag accgtggtcg acttccgtga tgtcagccgg tccaagtcga
     3241 tcgaggagag caaggatctg ttcctcgcgg tgaccagtca tgaactgcgg acgccgatca
     3301 ccgtcgtcca ggggtatgcc tcgacgttgc tgacccactg ggagaacatc accgacgaag
     3361 agcgtcgtgg atcggtggag cgtattgccg agcgcacccg cgcgctcgcc gcgctggtcg
     3421 agcagttgct gttgggttcg cgcgcgggtc tggctgcgcc gatgccctcc cacgtcatgt
     3481 tcgaccttgg aggtttgctg cgcaccgccg ttgccgggtt caagaatgtc gcgcaagggc
     3541 acgagttcgt gctcgacgtt gcgcccgacg tgccgctggc ggtcggcgat ccgagttcga
     3601 tcgagatcgt cttcagtcaa ctcctggaaa acgcgatcaa gtactccccc gacgggggcg
     3661 ccatcacagt gtcggtccgc acggaggggg agacgatcat cgcgcaggtg tgcgatcgcg
     3721 gcctcggcat cccgaccggt gaacacggac aggtattcga acgcttctac caagtcggcg
     3781 gcgagcggcg ccggttcggc ggagtgggtc tcggcctcta catcgtgcag cggctgctcg
     3841 aagcgcacgg cggcgcggtc cgagcccagc cgcgggacgg cggaggcacc tgtttcgagg
     3901 tgacacttcc tgggggatga agggcagtct gaagggcagt gcgtgaagcg ccatgtatga
     3961 ggttttgacc ccgtttggcg gggaacgtac ggccggtgag ctctcttcgc gtgacggcga
     4021 acccggcgag cgtcggatac gtgcgggccg tcctgcgcga tgatcttgca gtcctgccag
     4081 aaccggtccg cgaagaggtc gcgttgatcg cgaccgaact gctcgggaat gctgtcttgc
     4141 atgcgcgcgc tctcgacgac ggccagctgt cgatcgcgtg gggcgtcggc gagtacggtg
     4201 tcgagatcgc ggtcaccgac gggggcgcgc cgacccaccc gaccgtggcc gagccgggtt
     4261 cagaagacac gagcggccgt ggtctctcga tcgtcgcgac gcttgctgct cgttggggcg
     4321 tcgagcagca tgattcctcg acgaccgtgt gggcggtcat ccccttgacc gagcgggagt
     4381 ggatcgaccg tgagctgaac agccgtgagt cgatcagcct tggcagcaag gtggccgcgc
     4441 gcgcgttgta gtgttcccct cgcccgggta ccaccgcatc ccccgtcggt cgcacccgag
     4501 cgcccacgag acccccggga gcgccccatc tctgcacctc gcgcggtgat caccgggggc
     4561 gccgggttcc tcggctccca cctgtgcgag cgcctcctgc gtgacgagta cgaggtgctg
     4621 tgcctcgata acttcctcac gggtacgccg aacaatgtcg agcacctgct cggcaatgat
     4681 cgcttccgcc tgctgaaggt cgatgtcacc gactggatcc atatcagcgg gccggtcgat
     4741 gccgtcctgc actttgcctc gccggcatcg ccagccgact atctccagtt gccgatccag
     4801 accctcaagg ttgggtcggt cggtaccttc cacgcggtcg gtctggcgca ggagaagggc
     4861 gcgcggttcc tcctcgcctc gacatcggag gtctatggag acccccaggt gcatccgcag
     4921 ccggaaacct actggggtca cgtgaatcca atcggcccgc gcggcgttta cgacgaggcc
     4981 aagcggtttg cggaggcgat gacggtcgcg taccgcgatc accgcgaggt cgatgcggcc
     5041 atcgttcgga tcttcaatac cttcggtccg cggatgcgcc ccgacgacgg gcgtgccatc
     5101 ccgaccttta tcagccaggc actgcgcggc gagccgatca cggtggccgg cgacggtagc
     5161 cagacccgct cggtctgcta cgtcgacgac ctcgtcaacg gcatcgtccg catgctgcag
     5221 tcggacttgc ccgggccggt caatctcggc aatccgcacg agctctcggt tctcgagctg
     5281 gcggaactca tccgcgatct cgccggctcg tcgtcgtcca tcgagttcgt cgctttgccg
     5341 caagacgatc cgctggtgcg gcgtcccgac atcaccgtgg cgacgcgcga cctgggctgg
     5401 tcgccgcagg tcgacgtcga ggacggcctg cgtcgcacga tcgactggtt tcgcacccat
     5461 cccgatctgg tcggcatcag ctagcgtcgc gtcatggcac tcctaaccga ggctgtggtg
     5521 gtgatctccg gcgccggtcg cggcatcggc cgcggcgagg cgatcgaatt ggcgcgcgat
     5581 ggcgcgcggg tcgtcgtgaa cgatgtcgac gctgaggccg cgtccgccgt ggtcgccgac
     5641 atcaac
//
LOCUS       DATCBJ010000222         4251 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_522831, whole genome shotgun sequence.
ACCESSION   DATCBJ010000222 DATCBJ010000000
VERSION     DATCBJ010000222.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 4251)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4251)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4251
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_522831"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            195..347
                     /locus_tag="VII50_04495"
     CDS             195..347
                     /locus_tag="VII50_04495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5050137.1"
                     /translation="MAGDRLFIGYPHPSPTALGGTERSVKVDLNSESDGIELYGGVSS
                     FFLDRS"
     gene            complement(344..1273)
                     /locus_tag="VII50_04500"
     CDS             complement(344..1273)
                     /locus_tag="VII50_04500"
                     /inference="COORDINATES: protein motif:HMM:NF041635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STM3941 family protein"
                     /protein_id="HEY5050138.1"
                     /translation="MISSPPDSRDVRIRGGRTAVTLLLVCAAFVVVGAALIAVPHASV
                     GAKIAGALTIAVFGPLPLYAGLRMLRSGGVYVLGSDGIRFPLNGWPVLPWSDIQGTRI
                     VVRRRRRYLVVDTPTANARVRQMKSGSRAARQNLRIGLGLMPIPEQMAPTSLEELQRK
                     IERRRTGSAASTSRAASGSSDTTILPASAMRAPTSNRSAWQGSSQALRNVAAGNALVL
                     ILAMLRHHHVRTPQALIAAIVAALLIGALAVHLEAVFTGWVTIVTAEIVLVVVDLTVG
                     QVAVASRLLDLFFPFCVLFVAAQAWPQHRRSRS"
     gene            1492..1565
                     /locus_tag="VII50_04505"
     tRNA            1492..1565
                     /locus_tag="VII50_04505"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1524..1526,aa:Gly,seq:ccc)
     gene            complement(2087..3148)
                     /locus_tag="VII50_04510"
     CDS             complement(2087..3148)
                     /locus_tag="VII50_04510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5050139.1"
                     /translation="MDDEPIRSQAARAAAELALVRVVHHYGDRPEFVVLGGLVPQLLC
                     SDAGVAHVGTTDIDVQVDLEIAGGVVNTGRLEQALRNAEFVPDRERVWRWTTVAGGVG
                     AVVKFELLADLETEPNGATVIFDDCEQLGAANLRGTGFAARDVEVRRISAKDGGVWSN
                     VEVNVTGLAGFLLAKVAAAHGRRKAKDGTTSPSSSCTTTRAVRKRRSNWCSTHSARIS
                     AVSQLPYWTWPRTSPILARKGQPPTPSSSASTTPMKIPQHSRQTHWLPSRRSARHCFG
                     ISRLLDVDAFIDGRGPLVAALRRADYDGGRAHGGTRGGAAGSKRCGVGWTGSHGRHSM
                     QLLSLRRRRTCRCRRTATR"
     gene            complement(3141..4148)
                     /locus_tag="VII50_04515"
     CDS             complement(3141..4148)
                     /locus_tag="VII50_04515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5050140.1"
                     /translation="MSADRLLAAVRAVLPSSVPVDVIRDAGTLTDLRIGRSRVRAGWL
                     LPGWPRQVRDALTTLPQPELLVAPVMSPGARALLDKAGVSWIDETGAAQLAIGSLFVS
                     RDGSPPRAPATKASWTRATLGVAEAVLTGTEPTGSAVVHTTSLSVGSVSRALAYLAES
                     GFLTADQPRGRLSARRTLDADGLLDSYAEAVPALRAHLKLRVGVVWRDPTREVIEAAS
                     GWEQAARAWAATSSLAAAVLAPFLTQITPLEIYVEAMSLADLQLAAREIGARPVEGGR
                     LVLRPFPGSATERLSEKIGDGVRCVPWPRVYADLRQTGVRGEDAAEHLREHFMKEPAH
                     G"
BASE COUNT          737 a         1378 c         1394 g          742 t
ORIGIN      
        1 ggctcggtgg tcaattgctc ggtggtgagg ccgttcgtga tggcgcgcca cttctcgacg
       61 gtcgctccga gggtggccac cgggtcggtg tcaccgatga gatcgagcag cgcctgcttg
      121 taccgcgcag gttgagctgt cgcgtcgggg atcaggatgg gttccgttgc catggtttcc
      181 tccgcgcgca tggggtggct ggtgaccgac tgtttatcgg ttacccgcat ccgtcgccaa
      241 ctgcattagg cgggactgaa cgatcggtga aggtcgacct gaacagcgag tcggacggca
      301 tcgagttgta cggcggggtg tcgtcgttct tcctcgaccg cagctagcta cgcgatcgcc
      361 gatgctgcgg ccatgcctga gcggccacga ataggacgca gaacgggaag aagagatcga
      421 gtaaccggga tgcgacggca acctggccca cggtcagatc cacgacgacg agcactatct
      481 ctgcagtcac gatggttacc caccctgtga acaccgcttc gagatgaact gccaacgcgc
      541 cgatcagcag cgccgcaacg atcgcggcga tgagcgcttg gggcgtccgg acatggtggt
      601 gtcgcagcat ggccaaaatg aggacgaggg cgttgcccgc ggcaacgttg cgaagcgctt
      661 gtgagcttcc ctgccaggcg ctccggtttg acgtgggcgc gcgcatcgct gaagccggga
      721 ggatcgtggt gtcggaactc cccgacgccg cccgcgacgt cgacgcagcg ctgccggttc
      781 ggcgacgttc gatcttcctc tggagctcct cgagggacgt tggtgccatc tgctcaggaa
      841 tcggcatcag cccgagcccg atgcgcaggt tctgccgtgc cgcgcgtgat ccagacttca
      901 tctgccgcac tctcgcgttc gcagtcggcg tatcgaccac gaggtagcga cgacgccgcc
      961 gcacgacgat cctggttccc tgaatgtcgg accaaggcag cactggccac ccattgagcg
     1021 gaaaccgaat gccgtctgag ccaagaacgt agacaccgcc ggatcggagc atccgcaggc
     1081 ctgcatatag gggcagcggt ccgaagactg cgattgtcag cgcacccgcg atcttcgctc
     1141 cgacgctggc atgcgggacg gcgataaggg cggccccgac gaccacgaac gcggcgcaga
     1201 ccagaaggag ggtgaccgcc gtccgaccgc cacggatgcg gacatcgcgt gaatccggag
     1261 gagacgaaat catcatgcct tcctatcggg acccgagtgc cgttccgaca gacgtgccgg
     1321 gtcgtgcgct gatcagcttg gtgccggccg catactcctg acgcgcgccg tctccgagca
     1381 cccacaccga ctgctggccc tgatcgccca ctttgccgtt ggtcggggcg gtgacggcgt
     1441 cggtagagtg aggcggtcgt cagggccgga tggcttggcc tggcggcgct tgcgggtgta
     1501 gttcaatggc agaacatcag cttcccaagc tgacagtgcg ggttcgattc ccgtcacccg
     1561 ctccagcgga aaaccgcctc tgacctgcgg gtttacccga caccaagatc acggtatctg
     1621 cccgcccggg ccttccagtg cccgtgtttg gccctgagtt gttccacgtt tgttcgacgc
     1681 cgggcccgga gtgactcttc ccggcaccgt tgtgcgacat cgctgactag ccggccaggg
     1741 gccgcagaag attggtgacc acgtaggact cgccgcggat gtcgaaggtc gcagccgagg
     1801 attccagctc tccgatggta cgaacctcag cggtcagcga cccgatcgaa tcgatgaacc
     1861 tagccgcctt cacgcgcacg gctggcccat gatcgtcaga gaagcccgcg ctacgaagcc
     1921 gatacgagcg agaatcggag ccgagtagtc agaggcatgt atcgcaagag tgccgcttcc
     1981 tgcctcctgc gcgaccagcc accgacgatg caccagtgct cggtaacagc ctcggcctcg
     2041 cgcgtggggg cgagtggcgc ttcccgctag gacagcgaag ccttggttac cgagttgcag
     2101 tccgccggca gcgacaggtt cgccgtctgc gaaggctaag aagttgcatc gagtgccgtc
     2161 catggcttcc agtccagccc acgccgcatc gtttcgatcc agcagctccg cctcttgttc
     2221 caccgtgagc tcgcccgccg tcgtagtcgg ctctgcgcag tgctgccacc agtggcccgc
     2281 ggccgtcgat gaacgcgtcg acgtcgagca ggcgactaat gccgaagcag tgcctggcag
     2341 aacgtctcga cggcaaccag tgcgtctgcc gcgagtgttg aggaatcttc atcggggtgg
     2401 tcgatgccga actgctcggc gtaggcggtt ggcccttgcg cgcgaggatc ggagaagttc
     2461 gcggccaggt ccagtaaggc agttgtgaga ccgccgagat ccgcgccgaa tgtgtcgagc
     2521 accagttgga ccgccgcttg cggaccgccc gcgtcgttgt gcaggaggac gaaggcgatg
     2581 tcgtaccgtc cttcgcttta cgtcgtccgt gcgcggcggc gactttcgcg agcaggaagc
     2641 cggcgagacc ggtcacgttc acctcgacgt tgctccacac cccgccgtcc tttgcggaga
     2701 ttcgccgcac ctcgacgtcg cgagcggcga agccggtgcc gcgcaggttc gctgcaccta
     2761 gctgttcgca gtcgtcgaag atgacggtgg cgccgttggg ttcggtctcc aagtccgcga
     2821 gcagctcgaa cttcacgaca gctccgacgc cgccggcaac ggtggtccac cgccacactc
     2881 gctctcgatc cggtacgaac tcggcgttgc gcaaggcctg ttcgaggcga ccggtgttga
     2941 cgactccgcc cgcgatctcc aggtcgactt ggacgtcgat gtcggtcgtg ccgacgtgcg
     3001 cgacgcctgc atcagagcac agcagctggg gcacaagccc gccgaggacg acgaactcgg
     3061 gccggtcgcc gtagtggtgt accacccgaa cgagagcaag ctccgcggcc gcgcgtgcgg
     3121 cctgggaccg tatcggttcg tcatccatgc gcaggctctt tcatgaagtg ctcccgaaga
     3181 tgctcggcgg cgtcctcgcc acgaaccccg gtctgccgaa gatccgcata gacccgcggc
     3241 cacggcacgc accgcacgcc gtcaccgatc ttttcggaga ggcgctcggt ggccgatcct
     3301 gggaagggtc gcagcacgag gcgccctccc tctactgggc gggcaccaat ctctcgggct
     3361 gcgagctgaa ggtcggctaa tgacatggct tcgacgtaga tttcgagcgg tgtgatctgc
     3421 gtgagaaaag gtgcgagtac tgcggccgcg agtgagctgg tcgccgccca cgcccgggcc
     3481 gcctgctccc agccggatgc cgcctcgatg acttccctgg tcgggtctcg ccacacgacg
     3541 ccgacacgca gcttgagatg tgcgcgcagc gcgggtaccg cctcggcgta ggagtccagg
     3601 agcccgtcgg cgtcaagggt tcggcgcgct gacagccggc cgcgcggctg gtcggcagtg
     3661 agaaagccgc tttcggccag gtaagccaag gcccggctga cggatccgac ggacagacta
     3721 gtggtatgca cgactgctga ccctgtcggt tcggtaccgg tcagcactgc ctcggcgacc
     3781 ccgagcgtgg cacgcgtcca cgaagccttg gtcgcaggcg cgcgtggtgg cgacccgtcg
     3841 cgggacacaa agagtgaacc aatggccagc tgggctgcgc ctgtctcatc gatccagctg
     3901 accccggcct tgtcgagcaa tgcccgtgcg cctggcgaca tcaccggtgc gaccagcagc
     3961 tcaggttgcg gcaaggttgt gagagcgtct cgcacttggc gtggccaccc aggtaggagc
     4021 caaccagcgc gaacacgcga tcgtccgatc cgaagatcgg tgagtgtgcc cgcgtcgcgg
     4081 atgacgtcga ccggtactga cgacggaagg acagctctca ccgctgccag cagccgatcg
     4141 gcgctcatcc cgactcctaa cttccatgac gcagcatacc tcactacatc agtgatgtat
     4201 gctaaccact gagctttcga tcccctaagg catgtttgcg acgtgctcgc g
//
LOCUS       DATCBJ010000223         2293 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_123949, whole genome shotgun sequence.
ACCESSION   DATCBJ010000223 DATCBJ010000000
VERSION     DATCBJ010000223.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2293)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2293)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2293
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_123949"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            234..1049
                     /gene="cysT"
                     /locus_tag="VII50_04520"
     CDS             234..1049
                     /gene="cysT"
                     /locus_tag="VII50_04520"
                     /inference="COORDINATES: protein motif:HMM:TIGR02139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter permease subunit CysT"
                     /protein_id="HEY5050141.1"
                     /translation="MPPWWAARSGKSSRGGTSFGVGLVTLYLSFLVLIPLGAVVWKSQ
                     SDGWSQGWHQVTSPEAWAALKLTLTISVGVALFNLVMGTIIAWVLVRDEFPGKGVVEL
                     FIDLPFALPTIVAGVVLLSLYGPRSPAHVDIAFTRTAIIVALLFVTLPFVVRTVQPVL
                     IELDRDMEAAAASLGASPFLIARRIIVPNLVPAMLTGTGLAFARAIGEFGSVSLLSGN
                     LPFHTEVSAVNIYNQIQSDNPVGAAAQSTVLLIIAFVVLLALDRLKRWGVHRG"
     gene            1042..1887
                     /locus_tag="VII50_04525"
     CDS             1042..1887
                     /locus_tag="VII50_04525"
                     /inference="COORDINATES: protein motif:HMM:TIGR00969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter permease subunit"
                     /protein_id="HEY5050142.1"
                     /translation="MASASEGAVVVRGNRRPVARYGLRFVALGYLLVLLAAPVAMVFY
                     RAFQHGLGPIWSALTTSEARSAIEVTLIVAISAVVLNTLFGVGTALLLVRRRFPGRHL
                     LDVLVDLPQAVSPIVVGLALILVYGKFGFFGSWIERNGIKIIFSLPGMVLATAFVSLP
                     LVVRSVAPVLAELGDEQEQAARTLGASSWQTFRRVTLPSIRDALAYGVVLSLARSLGE
                     YGAVTVVSGHVIGKTLTLTLFIDERLQNFDQPAAYSAAVLLAIIAMGSLLISRRFRTR
                     SRSAA"
     gene            1884..>2293
                     /locus_tag="VII50_04530"
     CDS             1884..>2293
                     /locus_tag="VII50_04530"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY5050143.1"
                     /translation="MSISIRHVTKRFAQFIALDDVSVDIPTGSLTALLGPSGSGKSTL
                     LRVIAGLDTADTGTVSIEGADATHLPPQRREVGFVFQHYAPFKHMTVYDNIAFGLTVR
                     KAGKAETKSKVNELLELVHLEKFQTRYPDQLSGG"
BASE COUNT          357 a          693 c          752 g          491 t
ORIGIN      
        1 gatctacgcg caggccaagg ggcaggcggt cgactacgta atcccgagtg acacgctgct
       61 gatcgagaac ccggtcgccg tcacgacgac cacgaagttc ttcgacccga cgaatggtgt
      121 gatcacggca atcgagaagt ccaacggtgt cgctaccacc aaatagcacg cgggccgacg
      181 caagcgggcc gggcgttcac gcccagtccg cttcgccggg tattggtgca cccgtgccgc
      241 cgtggtgggc ggcacgatcg ggcaagagtt cgcgcggggg aacgtcattt ggggtagggc
      301 tcgtgacgct gtacctcagc ttcctggtgt tgatcccgct cggtgcggtc gtctggaagt
      361 cacagagcga cggctggtcg caggggtggc atcaggtcac gtcgccggag gcgtgggctg
      421 cgctcaagct cacgctgact atttcggtcg gtgttgccct cttcaatctc gtgatgggca
      481 cgatcatcgc gtgggtactg gtgcgcgacg agttccctgg caagggagtc gtcgagctct
      541 tcatcgactt gccgtttgca ttgccgacga ttgttgcggg cgtcgtgttg ctgtcgctgt
      601 atgggccgcg aagcccggcg cacgtcgaca tcgcgtttac ccgaacggcc attatcgtgg
      661 cgctgttgtt cgtcacgttg ccattcgtgg tgcgaactgt gcagcccgtg ttgatcgagc
      721 tcgatcgcga catggaggcg gccgccgcgt cacttggcgc ttcgccattt cttattgcgc
      781 gccggatcat cgtgccgaac ctagtgcctg cgatgttgac tggaaccggg cttgcgttcg
      841 cacgggcgat tggcgagttc ggttcggtca gcctgctctc gggcaacttg ccgtttcaca
      901 ccgaggtatc cgcggtcaac atctacaacc agatccaaag cgacaatccc gtgggagccg
      961 cggcacagtc gacggtgttg ctcatcatcg cgttcgtcgt cctgctcgca ctcgatcggc
     1021 tgaagcgctg gggtgtgcat cgtggctagc gcatcggagg gcgctgtagt ggtgcgcggc
     1081 aaccgccgcc cagtcgcgcg ttatgggctg cgcttcgtcg ccctgggcta cctactcgtg
     1141 ctgcttgccg cgccggtcgc gatggtcttt tatcgcgcgt tccagcatgg gctcggaccg
     1201 atctggtccg cactgacaac aagtgaggcg cgcagcgcta tcgaggtgac gctgatcgtc
     1261 gccatttcgg ctgtcgtact gaacacgttg ttcggcgtcg gtacggcgtt gttgcttgta
     1321 cgccggcgtt tcccgggacg ccatctcctc gacgtcctgg tggatcttcc tcaggcggtc
     1381 tcgccgatcg tcgtcggcct tgcgctgatc ctcgtgtacg ggaagttcgg cttcttcgga
     1441 tcctggatcg agcgcaacgg aatcaagatc atctttagcc tccccgggat ggtgctggcg
     1501 acggcgttcg tctcgctgcc cctggtcgtc cgctcagttg ctcctgttct tgccgaactc
     1561 ggtgatgagc aagagcaggc cgcgcggacg ctcggtgcat cgagctggca gacgtttcgc
     1621 cgggtcacgc tgccgtcgat tcgcgacgcg cttgcctatg gtgtggtgct gtcgctggcg
     1681 cggtcgctcg gcgagtacgg agccgtgacg gtggtgtcgg gacatgtgat cggaaagacg
     1741 ctgaccctta cgctgttcat cgacgaacgg ctacagaact tcgaccagcc ggcggcctat
     1801 tcggcggccg tcctgctggc gatcattgcg atgggcagtc tgctgatcag caggcggttc
     1861 cggacgcgaa gcaggagtgc cgcatgagca tctcgattcg ccacgtgacg aagcgctttg
     1921 cgcagttcat tgcgctcgac gatgtcagcg tcgatattcc caccgggtcg ctgaccgcgc
     1981 tgctgggtcc gagtggcagt ggcaagtcga cgttgctgcg cgtcatcgcc ggcctcgaca
     2041 ccgccgatac cggcacggtc agcatcgagg gcgctgacgc gacgcacctg ccgccgcagc
     2101 gccgcgaggt cggttttgtc ttccagcact acgcgccgtt caagcacatg accgtgtacg
     2161 acaacatcgc gttcggcctc acggtacgca aggcgggcaa ggccgagacg aagtcgaagg
     2221 tgaacgagtt gctcgaactc gtgcacctgg agaaatttca gactcgctat ccagatcagc
     2281 tttctggtgg gca
//
LOCUS       DATCBJ010000224         8855 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_538465, whole genome shotgun sequence.
ACCESSION   DATCBJ010000224 DATCBJ010000000
VERSION     DATCBJ010000224.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 8855)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8855)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8855
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_538465"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..656
                     /locus_tag="VII50_04535"
     CDS             <1..656
                     /locus_tag="VII50_04535"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HEY5050144.1"
                     /translation="GLLVTGLLGASDASGGTVDGIACCSTVPSALREVRSMCERWFDA
                     VSLVVVEPGIRTGVPVLYDNPKEVGADRIVNTLAAFTLHGGPAIVVDFGTSTNFDVVS
                     AKGEFLGGVLAPGIEISVDALASRAAQLMKVQLVRPRSVIGKNTVESLQSGIIYGFAS
                     QVDGITERIIAELETPATVIATGGLATVVLEECRLIDVHEPWLTLIGLRLMFERNIA"
     gene            688..2172
                     /gene="lysS"
                     /locus_tag="VII50_04540"
     CDS             688..2172
                     /gene="lysS"
                     /locus_tag="VII50_04540"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019044649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HEY5050145.1"
                     /translation="MSDDLPEQMRVRREKIDWLRAQGIEPYPAGFPRTATIERVRAAH
                     VDLPPDTATGDRVGVVGRVVLARNSGKLCFATIRDGSGELQVMISLDRLGADSLALWK
                     SAIDLGDHVGIEGEVITSRSGELSVLADSWALTAKCLRPLPDKHRGLTDPESRVRQRY
                     VDLIANPDAREMVRIKAKVVGSVRETLARHGYLEVETPTLQLLHGGAAARPFQTHLNA
                     FDIPMYLRIALELYLKRIVVGGIERVYEIGRIFRNEGVDSTHSPEFTMLEAYEAYGDY
                     DTMAALTHDIVVGAALAVGSTVVPDGRGGAIDLAAPWRTMTIHQAVSLAVGEEISIDT
                     DVEKLRTISARYDVALPPGQSGHDHGHIVLELFEKLVEHTLVEPTFVRDYPVSVRPLA
                     RPHRDDPRLVEAWDLVVNGVELAPAYSELVDPIEQRRRLTEQSLLAVGGDPEAMELDE
                     DFLRALEYGAPPMGGLGVGIDRLVMLLTGASIRETILFPLVRPE"
     gene            2176..2703
                     /locus_tag="VII50_04545"
     CDS             2176..2703
                     /locus_tag="VII50_04545"
                     /EC_number="2.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino-acid N-acetyltransferase"
                     /protein_id="HEY5050146.1"
                     /translation="MTSPPADIAVRRARTADVKAIRGFVDAYAPERKLLNKPTVTLYE
                     DVQEFWVAERLAGNAPPSVVGCGALHVMWEDLAEVRTLAVDPSMRAKGIGHLLLDQLL
                     RTARELGVRRLFCLTFEVDFFVAHGFSELEGSPVAPGVFEELLRSYDEGIAEFLDLER
                     VKPNTLGNTRMIKYL"
     gene            2761..3105
                     /locus_tag="VII50_04550"
     CDS             2761..3105
                     /locus_tag="VII50_04550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lsr2 family protein"
                     /protein_id="HEY5050147.1"
                     /translation="MAQKVQVTLVDDLDGSSADETLTFGLDGVTYEIDLSRGNAKKFR
                     DSLNAYVGAARRSGRAARAGRPRSRTGRRAAAGSQAPTSDIRAWARSKGIKVNERGRI
                     SADVVAKYEAAH"
     gene            3381..5927
                     /locus_tag="VII50_04555"
     CDS             3381..5927
                     /locus_tag="VII50_04555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009084262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HEY5050148.1"
                     /translation="MFERFTDRARRVVVLAQEEARMLNHNYIGTEHILLGLIHEGEGV
                     AAKALESLGISLEGVRQQVEEIIGQGQQAPSGHIPFTPRAKKVLELSLREALQLGHNY
                     IGTEHILLGLIREGEGVAAQVLVKLGADLNRVRQQVIQLLSGYQGKQEATAGGPAEGT
                     QSTSLVLDQFGRNLTQAAREGKLDPVIGREKEIERVMQVLSRRTKNNPVLIGEPGVGK
                     TAIVEGLAQAIVKGEVPETLKDKQLYTLDLGALVAGSRYRGDFEERLKKVLKEIRTRG
                     DIILFIDELHTLVGAGAAEGAIDAASILKPMLARGELQTIGATTLDEYRKHLEKDAAL
                     ERRFQPIQVAEPSLAHTIEILKGLRDRYESHHRVSITDGALVAAAQLADRYISDRFLP
                     DKAIDLIDEAGSRMRIRRMTAPPDLREFDEKIAGVRREKESAIDAQDFEKAASLRDRE
                     KQLLAAKSQREREWKAGDMDVVAEVDEELIAEVLATATGIPVFKLTEEETSRLLRMED
                     ELHKRVIGQEPAIKALSQTIRRTRAGLKDPKRPGGSFIFAGPSGVGKTELSKTLAEFL
                     FGDEDSLIQLDMSEFMEKHTVSRLFGSPPGYVGYDEGGQLTEKVRRKPFSVVLFDEVE
                     KAHPDIFNSLLQILEDGRLTDAQGRVVDFKNTVIIMTTNLGTRDISKGFNMGFSKEHD
                     AQGNYERMKAKVNEELKQHFRPEFLNRVDDTIVFHQLTEDEIVTIVDLMIAKVDLRLR
                     DRDMGLELTSDAKKVLAKKGYDPVLGARPLRRTIQREIEDVLSEKILFGELKAGQIVV
                     VDAEGVDTPEAAFTFRGEAKPALVPDLPPLAEAAPAADPATAPGAAPAAE"
     gene            complement(5956..6816)
                     /locus_tag="VII50_04560"
     CDS             complement(5956..6816)
                     /locus_tag="VII50_04560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009084234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HEY5050149.1"
                     /translation="MVGELLDWYSHAARRLPWRAPAATAWAVLVSEIMLQQTPVARVL
                     PAYEQWIARWPTPAALATAAPGDAVRAWDRLGYPRRALRLHATATTIVAIHGGQVPAD
                     YTQLRALPGVGDYTAAAVAAFAFRQRQVVLDTNVRRVLARAVSGIAEPSSAPTVAERR
                     VASELLPDGGERAAAFSVALMELGALICRARAPQCSQCPVADRCAWRRAGYPPSALPP
                     RRGQGYEGTDRKARGAVLAVLRAQPGPAHVRQLRSAWPDDVQLDRALDGLIADGLVEP
                     LARDRFRLPT"
     gene            6937..8079
                     /gene="cheB"
                     /locus_tag="VII50_04565"
     CDS             6937..8079
                     /gene="cheB"
                     /locus_tag="VII50_04565"
                     /EC_number="3.1.1.61"
                     /inference="COORDINATES: protein motif:HMM:NF001965.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0008984 - protein-glutamate methylesterase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis-specific protein-glutamate
                     methyltransferase CheB"
                     /protein_id="HEY5050150.1"
                     /translation="MSVAREASTPASAQAPQPDAQEQSAVTVVLVDDSGIERRFLRAA
                     ITADPGLCVVGEARTGRDAIALIERLRPSAVVLDLELPGTAAIEIIERIMASVPTPIL
                     VCSTEGDAARAAAALAAGAIDVVARPTARDADRASYAEALRRSVRVASRVRVITHPRG
                     RLKTPSGVQSASGASSALSPRTPAEGTVDLVAIGASTGGPQALAHLLGALPGDFTPAV
                     VVVQHMADGFIEGLATWLDGLCALPVGVATSGTRLQQGTVTIAPSARNLVVNDHLRIS
                     IEQPPPTQFNVPGVDATFESVARAVGPRAIGVLLTGMGRDGAVGLKAMRDAGAITIGQ
                     DEESSAVWGMPAAAAACGAVQRQLPLFEIAGALVAILHGDSFGGER"
     gene            8076..>8855
                     /locus_tag="VII50_04570"
     CDS             8076..>8855
                     /locus_tag="VII50_04570"
                     /inference="COORDINATES: protein motif:HMM:NF013867.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008983 - protein-glutamate
                     O-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HEY5050151.1"
                     /translation="MKFSLTDEEFDTLRKVLEKSAGLVHDESRRDSLTFAVAGRIEAI
                     GASSPAGYLAMLGGPGGAAERQQLIDVVTIQETHFFRNPPQIRALRQHVLPELIRRST
                     ALNRPLTIWSAGCSTGEEPYSLAMLVRELMPMATREHVRIVGSDVSATALAFADAGRY
                     GARAVQMADPIDLGRWFDIDRGWYSVKDDVRELVDLQLHNLITEAPPFESSEVDLLLC
                     RNVTIYFNRTTTKALMSRFHTALGDGGYLFLGHSETLWQMSE"
BASE COUNT         1540 a         2793 c         3005 g         1517 t
ORIGIN      
        1 tggggctgct cgtcaccggc ctgctcggag catcggatgc cagtggtgga acggtcgacg
       61 ggatcgcgtg ctgctcgaca gtgccgtccg cgttgcgcga agtgcggtcg atgtgcgagc
      121 gctggttcga cgcggtgtcg ctggtcgtcg tcgagcccgg tattcgtaca ggggtgccgg
      181 tgctctacga caacccgaag gaggtcggcg cagatcgcat cgtgaacaca cttgccgcgt
      241 tcaccctgca cggcgggccg gccatcgtcg tcgacttcgg gacgtcgacc aacttcgacg
      301 tcgtatctgc gaagggtgag tttcttggcg gtgtcctcgc gcccggcatc gagatctctg
      361 tcgatgcgct ggcctcgcgc gcggctcaac tgatgaaggt ccagttggtg cggccgcggt
      421 cggtcatcgg caagaacacc gtcgagtcgt tgcagtcggg aatcatctac gggttcgcct
      481 cgcaggtcga tgggatcacc gagcggatca tcgccgaact cgaaacgccg gccacggtga
      541 ttgccaccgg cgggctcgcg actgtggtgc tcgaggaatg ccggctcatc gacgtccacg
      601 agccgtggtt gaccttgatc ggcttgcggc tgatgttcga acgcaatatc gcatgaggtc
      661 gcccgcctgg ccgatagcct tccgcccgtg agtgacgatc tgcccgagca gatgcgggtc
      721 cgccgcgaaa agatcgactg gctgcgggcg cagggtatcg agccgtatcc ggccggcttt
      781 ccgcggacgg cgaccatcga gcgggttcgc gcggcgcacg tcgatctgcc acccgacacc
      841 gcaaccggtg atcgcgtcgg cgtcgtgggt cgagtcgtgc tcgcgcgcaa ctccggcaag
      901 ttgtgtttcg ccaccatccg cgatgggtcc ggcgagcttc aggtgatgat ctcgctcgac
      961 cggctcggtg cggactccct tgccttgtgg aagtccgcga tcgatcttgg cgatcacgtc
     1021 ggtatcgagg gtgaggtcat cacctcccgc agcggcgagc tttcggtact cgccgattcg
     1081 tgggcgctga ccgcgaagtg cctgcggcca ctgccggaca aacaccgggg actcaccgac
     1141 cccgaatcgc gggtcaggca gcgctatgtc gatctgatcg cgaaccccga tgctcgcgag
     1201 atggtcagga tcaaggcgaa ggtggtcggg tcggtccgcg agacgctcgc ccgccacggg
     1261 tatctcgagg tcgaaacccc gacattgcag ctgctgcacg gcggcgccgc ggcccggccg
     1321 ttccagacgc acctgaacgc cttcgacatc ccgatgtacc tgaggatcgc gcttgagctc
     1381 tatctcaagc ggatcgtcgt cggtgggatc gagcgggtct acgagatcgg ccggatcttt
     1441 cgcaacgagg gcgtcgactc gacccacagc cccgagttca cgatgctcga ggcatatgag
     1501 gcctacggcg actacgacac gatggctgcc ctgacgcacg acatcgtggt cggtgcggcg
     1561 ttggctgttg gttcgacggt ggtgcccgat ggtcgtggag gggcgatcga tctcgccgcg
     1621 ccttggcgga ccatgacgat ccaccaggca gtgtcgctgg cggtcggtga ggaaatctcg
     1681 atcgataccg acgtagaaaa gctgcgcacg atatccgcgc gatatgacgt cgcactgccg
     1741 cccggacagt ccggccacga tcacggccat atcgtgctcg agttgttcga aaagctcgtt
     1801 gaacacaccc ttgtggagcc gacgttcgtg cgggattacc cggtgtctgt ccggcccctc
     1861 gcgcgaccac acagagacga cccgcgattg gtggaggcgt gggatcttgt cgtgaatggg
     1921 gtcgaactcg cccctgccta ctccgaattg gtcgacccaa tcgagcagcg caggagattg
     1981 accgaacagt cgctattggc agtcggcggt gaccccgagg ccatggagct cgacgaggat
     2041 ttcttgcgcg cgctcgaata tggcgcgcca ccgatgggtg gtcttggtgt tggtatcgat
     2101 cggctggtca tgctgctgac cggggcgagc attcgcgaga cgatcctttt cccgttggtc
     2161 aggcccgagt aagcgatgac ttcgccaccc gccgacatcg ccgttcgtcg tgcccgcacc
     2221 gcggatgtga aggcaatcag ggggttcgtc gatgcatacg ccccagagcg caaactgctg
     2281 aacaaaccaa ccgtgacgct gtacgaagac gtgcaggaat tctgggttgc cgagcgattg
     2341 gcaggcaatg cgccgccatc agtggtcggc tgtggcgcgt tgcacgtgat gtgggaagac
     2401 ctggccgagg tacgaaccct tgccgtcgac ccgtccatgc gcgctaaggg catcggccat
     2461 ctgctgttgg accaattgct acgtaccgcg cgcgaactcg gcgtacgtcg gttgttttgt
     2521 ttgaccttcg aagttgattt cttcgtcgca catggatttt cagagctcga gggttcgccc
     2581 gttgcgcccg gggtgttcga agagttgttg cggtcatatg atgaaggcat tgcggagttt
     2641 cttgacctcg aacgtgtcaa gccgaacacg ctcggtaaca cgcgcatgat caagtatctg
     2701 taagacttgg cgctatattg caaatcgatg tctagttcct gtgtttgaaa ggacaatccc
     2761 gtggcgcaga aggtccaggt aacgcttgtc gacgacctcg acgggagttc ggctgacgag
     2821 acgctgacgt tcggtttgga cggcgtaacc tatgagatcg atctcagcag gggcaacgcc
     2881 aagaagttcc gcgattcgct gaatgcctac gtcggcgccg ctcgccgatc cggacgagct
     2941 gcacgcgccg gccgcccgcg tagtcgtacc ggccgcaggg ctgcggccgg cagccaggca
     3001 ccgacgtccg acatccgggc gtgggcgcgg agcaagggca tcaaggtcaa cgagcgcggc
     3061 cgcatctcgg ccgatgtggt tgccaagtac gaggcagccc attagctccc gacctgccgc
     3121 tagctggctg ttcgctggga gcgcaacggc ccgatccggc ggtgcggaac acccgcgggc
     3181 aggtcacagt tgggactcag tgagggcacg aacgccccaa tcgagcggtt tcacccgtcc
     3241 gaagaccgaa aattgggtct aggaacgcaa gtccgggcgc catagtggcg agaactctgg
     3301 cacgagcgcg ggaagggctg tccctggcgc cgggatagca tgaaaaagaa ggcaggcgcc
     3361 gccactgagg agcgagcgac atgttcgaga gattcaccga cagggcccga cgcgttgtcg
     3421 tgctggcgca agaagaagcc cgcatgctca accacaacta catcggcacc gagcacattc
     3481 tgctcggtct catccacgag ggcgaaggcg tcgccgcgaa ggctctggag agcctcggta
     3541 tctcactcga gggtgttcgc cagcaggtcg aagagatcat cgggcagggc cagcaggcgc
     3601 cgtccggtca catacctttc acgccgcgtg ccaagaaggt gctcgagctc tcgctgcgcg
     3661 aagctcttca gctcggccac aactacatcg gcaccgagca catcctgctc ggccttattc
     3721 gtgaaggcga aggcgtcgct gcccaggtgc tggtgaagct tggcgccgat ctgaaccgcg
     3781 tccggcagca ggtcatccag ttgctgtccg gctaccaggg caagcaagaa gcgaccgctg
     3841 gcggtcccgc cgagggcacc cagtcgacgt ccctcgtctt ggatcagttc ggccgcaacc
     3901 tgacgcaagc cgcgcgcgaa ggcaaactcg acccggtcat cggccgtgag aaggaaatcg
     3961 agcgcgtcat gcaggtgctg tcgcgccgca ccaagaacaa cccggtgctg atcggcgaac
     4021 ccggcgtggg caagaccgcg atcgttgaag gtctggcgca ggcgatcgtc aagggtgaag
     4081 tgcccgagac gctgaaggac aagcagctgt acaccctcga cctcggcgcg ctcgtcgccg
     4141 gtagccgcta ccgcggtgac ttcgaggagc ggctgaagaa ggtcctgaag gagatccgca
     4201 cccgcggcga catcatcttg ttcatcgacg agctgcacac gctggtcggt gctggcgctg
     4261 ccgaaggcgc catcgacgcg gcgtccatct tgaagcccat gctggccaga ggcgaactcc
     4321 agaccatcgg tgccacgacg ctcgacgagt accgcaagca tctggagaag gacgctgcgc
     4381 tcgaacggcg cttccagccg atccaggtcg ccgagccctc gctcgcgcac acgatcgaga
     4441 tcctcaaggg tctgcgcgat cgctacgagt cgcaccaccg cgtctcgatc acggacggcg
     4501 cactcgtcgc ggccgcgcag ctggccgatc gctacatcag cgatcggttc ctgcccgaca
     4561 aggccatcga cttgatcgac gaggccggct cacgcatgcg cattcgccgc atgactgcac
     4621 ccccggatct ccgcgagttc gacgagaaga ttgctggtgt ccgccgcgag aaggaaagtg
     4681 cgatcgacgc acaagacttc gagaaggcgg cgtcccttcg tgatcgcgag aagcaactgc
     4741 tcgcggcgaa gtcgcagcgc gagcgcgagt ggaaggccgg cgacatggac gtcgtggccg
     4801 aagtcgacga agagttgatc gccgaagtgc tcgcgaccgc gacgggcatt ccggtgttca
     4861 agctgaccga ggaagagacc tcgcggctgc tgcgcatgga agacgagctg cacaagcggg
     4921 tcatcggcca ggagccggcg atcaaggcgc tgtcgcagac gattcgtcgt acccgtgccg
     4981 gtctgaagga cccgaagcgt cctggcggct cgttcatctt cgccggcccg tcaggtgtcg
     5041 gtaagaccga gctctcgaag acgctcgccg agttcctttt cggtgacgag gactccctga
     5101 tccagctcga catgagcgag ttcatggaga agcacacggt gtcgcggctg ttcggttcgc
     5161 cgcctggtta cgtcggctac gacgagggcg gtcagctgac cgagaaggtg cgccgcaagc
     5221 cgttctccgt cgtgttgttc gacgaggtcg agaaggcaca ccccgacatc ttcaactcgt
     5281 tgttgcagat cctcgaggac ggtcggttga ccgatgctca gggccgggtt gtcgacttca
     5341 aaaacaccgt catcatcatg acgacgaacc tcggcacgcg tgacatctcc aagggcttca
     5401 acatgggctt ctccaaggag catgacgcgc agggcaacta cgagcggatg aaggcgaagg
     5461 tcaacgagga gctgaagcag cacttccggc ccgagttctt gaaccgtgtc gacgacacga
     5521 tcgtgttcca ccagctgacc gaggacgaga tcgtgacgat cgtcgacctg atgattgcca
     5581 aggtcgacct tcggttgcgc gaccgcgaca tgggcctcga actcacgtcg gacgccaaga
     5641 aggtgctcgc caagaagggc tacgacccgg tgcttggtgc gcgtccgttg cggcgcacga
     5701 tccagcgcga gatcgaggac gtgctgtcgg agaagatcct gttcggcgag ctcaaggctg
     5761 gccagatcgt cgtggtggac gccgagggag tcgacacccc cgaagccgca ttcacgttcc
     5821 gcggcgaggc caagccggcc ttggtccccg atctgccgcc gttggctgag gcggcaccgg
     5881 cggccgaccc ggcgactgct ccgggcgcgg cgccagccgc ggagtagtcg ggcgcggccg
     5941 aattgccgcg tagtactagg tcgggaggcg gaagcggtcg cgggccagtg gctcgacgag
     6001 gccgtccgct atcaggccgt caagggcacg atcgagctgg acgtcgtcgg gccaggccga
     6061 gcgtagctgc ctgacgtgcg cgggccccgg ctgtgcccgc agtaccgcga gcaccgcgcc
     6121 gcgagccttc cggtcggtgc cttcgtagcc ctggccgcgg cggggcggca aggctgaggg
     6181 cgggtaaccg gcgcggcgcc acgcgcagcg gtcggccacc gggcactgac tgcactgcgg
     6241 cgcgcgagcc cggcagatca aggcgcccag ttccatcagc gccacgctga acgccgccgc
     6301 ccgctcgccg ccgtccggta agagttcgct ggcgacgcga cgttcggcga ccgtcggcgc
     6361 gctcgacggt tcagcgatgc ccgataccgc ccgcgcgagt acccggcgca cgttggtatc
     6421 gaggacgacc tgccgttgcc ggaacgcaaa cgccgcgacg gcggccgcgg tgtaatcacc
     6481 gaccccaggc agggcgcgca gctgcgtgta gtcggcgggc acctgcccgc cgtgaatcgc
     6541 gacgatcgtg gtcgccgtcg cgtgcagccg caacgcccgc cgcgggtagc caagccggtc
     6601 ccaggcgcgg acggcgtcgc cgggcgcagc tgtcgcgagc gcggccggag tgggccatcg
     6661 ggcgatccac tgctcgtagg ccggcaacac ccgggccacc ggggtctgct ggagcatgat
     6721 ctcgctgacc agcaccgccc acgcggtagc cgcgggtgcg cgccacggca ggcgccgggc
     6781 ggcgtggctg taccagtcga ggagctcgcc gaccatcggc gcctctaggc tctgggcacg
     6841 ctcaggttgt cttaagaaag tcgccacatc agacgacagg ttaaggagtc attggggagg
     6901 agcccgcgcc gggcttgcgg accgaggagc gcctaggtgt cggttgcgcg cgaagcgtca
     6961 accccggcca gcgcccaggc cccgcagccg gacgcccagg agcagtcggc cgtcacggtt
     7021 gtgctggtcg atgactcggg gatcgagcgg cggttccttc gcgcggcgat caccgccgac
     7081 ccgggacttt gcgtggtcgg cgaggcgcga accggacgcg atgccatcgc gttgatcgag
     7141 cgcctgaggc cgtccgccgt ggttctcgac ctcgagttgc ccggcaccgc cgctatcgag
     7201 atcatcgagc ggattatggc cagcgttccg acgcccatcc tggtgtgctc gaccgaaggg
     7261 gacgctgccc gagcagccgc cgccctcgct gctggcgcga tcgacgtggt ggcgagaccg
     7321 acggcgcgag acgccgaccg ggcgtcgtat gcggaggcgc tgcgccggag cgtgcgagta
     7381 gcgtcgagag tgcgcgtcat cacccacccg cgagggcgcc tgaagacgcc aagcggtgtg
     7441 cagtctgcgt caggggcgtc gtccgccttg tcgccgcgaa ctcccgcaga aggcaccgtt
     7501 gacttggtgg cgatcggcgc gtcgaccggc ggaccgcaag cgctcgcgca tctgctgggt
     7561 gcgttgcctg gcgacttcac gccggctgtc gtggtcgtgc agcacatggc agacggtttc
     7621 atcgagggtc ttgcgacctg gctggacgga ctgtgtgcac tgccggtcgg ggtggcgacg
     7681 tcgggtactc gcctgcagca aggaactgtc acgatcgcgc cgagcgcgcg caacctcgtc
     7741 gtcaacgacc atctgcgcat cagcatcgag caaccgccgc cgacccagtt caacgtgccg
     7801 ggcgtcgacg cgacgttcga gagcgttgca cgcgcggtgg gtccgcgggc catcggtgtg
     7861 ctgctgaccg gtatgggtcg cgacggcgct gtcgggctca aggccatgcg agacgccggt
     7921 gccatcacga tcggtcagga cgaagaatcg agtgcggtgt ggggcatgcc ggcagcggcc
     7981 gctgcctgtg gcgccgtgca gcgccaactg ccgctctttg agatcgcagg tgcgctcgtc
     8041 gcgatcttgc acggtgactc gttcggtggt gagcggtgaa gttctcgctg accgacgagg
     8101 agttcgacac gctgcgcaaa gtcctcgaga agagtgccgg gctggtgcac gacgagagtc
     8161 gccgcgactc tttgaccttc gcggttgctg gtcggatcga ggcgattggc gccagttcgc
     8221 ccgccgggta tctcgcgatg ctcggtgggc ccggtggtgc ggctgagcgc cagcagctga
     8281 tcgacgtcgt cacgatccaa gagacccatt tcttcaggaa cccgcctcag atccgcgcgc
     8341 ttcgccaaca cgtcctgcca gaattgatcc gtcggtcgac ggcattgaat cgtccgctga
     8401 cgatctggag tgccggctgc tcgaccggcg aagagcccta cagcttggcc atgctggtcc
     8461 gcgagctgat gccgatggcg acccgcgagc acgtcaggat cgttgggtca gacgtctcgg
     8521 ccaccgcgct cgcattcgcc gatgccggcc gctatggcgc tcgcgccgtg caaatggcgg
     8581 atccgatcga tctgggccgt tggttcgaca tcgaccgcgg ctggtactcg gtcaaggatg
     8641 atgtgcgcga gctggtcgac ctgcaactgc acaacctgat caccgaagca ccgccgttcg
     8701 agagcagtga ggtcgatctg ttgctctgtc gcaacgtgac gatctacttc aaccggacga
     8761 cgaccaaggc gctcatgtcg cggttccaca ccgccctcgg cgatggtggg tacctgttcc
     8821 tcggccactc cgaaacgttg tggcagatga gcgag
//
LOCUS       DATCBJ010000225         4825 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_447605, whole genome shotgun sequence.
ACCESSION   DATCBJ010000225 DATCBJ010000000
VERSION     DATCBJ010000225.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 4825)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4825)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4825
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_447605"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            269..730
                     /locus_tag="VII50_04575"
     CDS             269..730
                     /locus_tag="VII50_04575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5050152.1"
                     /translation="MPPRRRVVPAASTAAACLLAVVVIGGCSSSTHMHGALTDREFKA
                     AITIARAEAYKDGATLTSATATVGPGTETQANVGPPCTSGTLLHIKLIGRFPHTAVTP
                     SPITDTASEGNDYSVSAELITADAASGKACSLAVQTGAVTPDPGATLLFTH"
     gene            complement(856..1653)
                     /locus_tag="VII50_04580"
     CDS             complement(856..1653)
                     /locus_tag="VII50_04580"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY5050153.1"
                     /translation="MSTTSSTTPVPAARRFPAINRRAARAMLLSEGRLLLRNPGVVIW
                     TAVLPVAAAIILGSIPAVRKPSTSFGGLSFFQVYQPILVFFALTLLAMQALPDVLTRY
                     REMGVLKRLRTTPVSPALLLCAQLTLILSVAIICGALMVVAPPLGGAPFPRNIGGFVI
                     SYLLGAWAMLGLGMVIASLFHNAKVAAGFGSFLFFVLQFFAGLWWQRPNMPTWLRDIS
                     NFTPSGAAVQALTDSSVGHWPRALYLIVLAVWGAATSRLAIRFFSWE"
     gene            complement(1650..2585)
                     /locus_tag="VII50_04585"
     CDS             complement(1650..2585)
                     /locus_tag="VII50_04585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009079721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY5050154.1"
                     /translation="MSVIEVTGLRKVYGERTVVNGVSFSVEPGEIFGILGPNGAGKTT
                     TVECIGGLRHRDGGSVTVAGMDPANEGAAFREVLGIQLQESRLPDKMTVIEALDLYAS
                     FYPEPHDPAELLERLGMVEQRKTYFGKLSGGQKQRLSVALALIGNPRVAILDELTTGL
                     DPAARRDVWDLLEDLRGDGVTVLLVSHFMEEAERLCDRVIVIDHGQIIAADTPEGLAA
                     SVQADQRMSFLPSTPVDPAVLRALPDVHSVNTVGERVVVTGTDNVASAVILGLHGQGI
                     SVGKLRVDQPSLDDAFVALTTSNRAPETSSETASR"
     gene            2847..3047
                     /locus_tag="VII50_04590"
     CDS             2847..3047
                     /locus_tag="VII50_04590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5050155.1"
                     /translation="MRLNTSMTGVVIPGLIAGVIYAVIAAATGASAVAAIIGALLVAA
                     VAIGIGFIFRSIFKRRAGSRRE"
     gene            complement(3160..>4825)
                     /locus_tag="VII50_04595"
     CDS             complement(3160..>4825)
                     /locus_tag="VII50_04595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5050156.1"
                     /translation="SPDGVTWQLTDPQVPVATQAADSFGYGEQIAATGVGAHAVELAL
                     PDIVTGSFGGDTLRSVDGGRTWNTLSAPLSTGIDVLPDGVTMYATGSASTDSCNGAVY
                     RSTDAGAHWALLPSSCQDRALYAVTFVDPEHGFAAGGTPAKYNGSELVEATDDGGRTW
                     QVRWSTPLDPGTGSGYGVDNEIVRLVFTDAQHGYALNGGCIDGQNGPCGGNLYTTSDG
                     GYSWHAAGREGLGMAAAGADVYLSGRGQRNGASLAISTDTGQHWTLHAGATQVSSQPV
                     AGTGEKLWWQTNLGTYTSNDTGERWTPAIAPALSSLPSYADPLQAAPPGDLLALDRST
                     GATWSSTDGATTAHQSFVVAPNDSVQAVALGQDGRAAAIIGYNDDCLSPQLIAETEQS
                     KPGWQPETRPATLYLSTDAGAHWNRAPTASLPFLFSAFAPGSLATSSSVLVAIDACTH
                     LQISSNDGTTWSTITAPNLATGCTASVFDKEIWLDCPASVAHSSDAGTTWTTYTTNPG
                     GVQQLYQPLQATGPDTAIISAAGSLWRTTDGGHHWTQTWPTLNGES"
BASE COUNT          810 a         1583 c         1596 g          836 t
ORIGIN      
        1 ccgcaagcag ggcgacccca acctgcaacg agacaagctg tctacgcacc attccccgtc
       61 ccccacggtt ggacgcctca gagttgacca aacaccctga cagcgccgcg aacttcgtca
      121 gcgtactcgg ccacattggc cttggttacc gccccgctgt ccccgttttg ccacgcgagc
      181 tcatcggagg aaaacgcgcg tcattgcggc aatgagagtg cgggagggag tggtgcagct
      241 tcgtgcgtct ccgtggaacg attcggtcgt gccgccacgt cgaagggtcg tgccagccgc
      301 atcgacagcc gccgcctgcc tactcgccgt cgtcgtgatt ggtggatgct caagctcgac
      361 ccacatgcac ggtgcactca ccgatcgcga gttcaaggcc gcgatcacga tcgcccgagc
      421 ggaggcgtat aaggatgggg ccacgctgac cagcgcaaca gccacggtcg gcccaggcac
      481 cgagacgcaa gcgaacgtag gcccaccgtg cacttcgggc acgctcctgc acatcaaact
      541 gatcggcagg ttcccccaca ccgccgtcac tccctcgccg atcaccgata cggccagcga
      601 gggcaacgac tactcggtta gcgctgaact catcaccgcc gacgccgcgt ccggcaaggc
      661 ttgctcgctc gccgtgcaga ccggcgcggt aacacccgat cccggcgcga cgctgttgtt
      721 cactcattga catttgctcg cagcggctcc gccctccccg gcacgacgga aacgcgcgtc
      781 aaagcggcat agcgtgcgtt tgttcggccc gcggcgcgtg gccctgagaa cgcgttgtta
      841 tccggtaccc ccggcctatt cccagctgaa gaagcggatc gctagccgcg aggtcgcggc
      901 cccccacact gcgagcacga tcagatacag ggcgcggggc cagtgcccga cgctggagtc
      961 ggtcaacgcc tgcaccgcgg cgccgctggg ggtgaagttg gagatgtcac ggagccatgt
     1021 cggcatattg ggccgctgcc accacagtcc cgcgaagaac tgcaggacga agaacaggaa
     1081 cgatccgaag ccagcggcga ccttggcgtt atggaacagg ctggcgatca ccattcccag
     1141 acccagcatc gcccacgcac cgagcaggta ggagatgacg aagccaccaa tgttgcgcgg
     1201 aaagggagcg ccgcccagcg gcggggcgac caccatcagc gccccgcaaa tgatcgcgac
     1261 agacaagatg agggtcagct gcgcgcacag cagcagcgcg ggcgagaccg gcgtcgtgcg
     1321 cagccgcttg agcacaccca tctcgcggta gcgcgtcagc acatccggta aagcctgcat
     1381 ggccagcagc gtgagcgcga agaacaccag gatcggctga tagacctgga agaagctcag
     1441 gccgccgaaa ctcgttgacg gcttgcgtac cgcagggatc gacccgagga tgatcgcggc
     1501 ggcgaccggc agaacagccg tccaaatcac cacgccgggg ttacgtagca gcagccggcc
     1561 ttcgctgagg agcatcgcgc gggcggcgcg ccggttgatc gccggaaagc gccgggcggc
     1621 ggggacggga gtcgtcgaac tggttgtact catcggctcg ccgtctcgct cgaggtctcc
     1681 ggcgcccggt tgctggtggt caatgccacg aacgcgtcgt cgaggctggg ctggtcgacc
     1741 cgtagcttgc ccaccgagat gccctgcccg tgcagaccaa gaatgaccgc actggcgacg
     1801 ttgtctgttc cggtaacgac tacacgctcg ccgacggtgt tgaccgaatg cacatccggc
     1861 agcgcccgca agactgcggg gtcgaccggc gtcgacggta gaaaactcat tcgctggtcg
     1921 gcctgcacgc tggccgccaa cccttcgggt gtgtccgcgg cgatgatctg gccgtggtcg
     1981 atgacgatta cccgatcgca aagccgctcc gcctcttcca tgaagtggga gaccagcagc
     2041 acggtgacgc cgtcaccgcg cagatcttcc aggaggtccc acacgtcgcg gcgggcggcc
     2101 ggatcgaggc cggttgtgag ctcgtcgagg atcgccactc tcgggttgcc gattagcgcc
     2161 aacgcgaccg acagccgctg cttctggccg ccggacagct tcccgaagta ggtctttcgc
     2221 tgctcaacca tgcccagccg ctccagcaac tcggccggat cgtgcggctc agggtagaaa
     2281 ctggcataca ggtcgagggc ctcgattacc gtcatcttgt cgggcagccg ggactcctgc
     2341 agctggatcc ccagcacctc ccggaacgcc gccccttcgt tggctggatc cattccggcg
     2401 accgtgacgc tgccgccgtc gcggtggcgc agtccaccga tgcactccac cgtcgtcgtc
     2461 ttgccggcgc cgttgggccc cagaatgccg aagatctcgc ccggctccac cgagaagctg
     2521 acaccattga ccacagtgcg ctcgccgtag accttgcgaa gcccggtcac ctcgatcaca
     2581 gacaccgctc cgtcctttcc aaccgggcgg gccgaaggca ccacgtgggc gtcatgacac
     2641 atccgcacga cgtacgatcc cacgcgacca tcggtgtggt gatagcgatc gtcacggtaa
     2701 tccccttcgc ttccgccgat tgatctcgtt gcgaatgggt tgactgatca aggcgccgaa
     2761 actcatcgct gcttgatgcg tagcagcggt ccaacggtaa cctcggagcc agcgaccggc
     2821 atccagcggc ggcggaaggg tcagccatga ggctcaacac atcgatgact ggtgttgtga
     2881 tcccggggct gattgccggg gtaatctacg ccgtcatcgc cgcggcgacc ggagcgtcgg
     2941 cagtcgccgc gatcatcggc gcgcttcttg tcgccgccgt cgcgatcggc atcggtttca
     3001 tcttccgttc catcttcaag cgcagggcag gatcgcggcg cgaataggcg ggcggcggca
     3061 gtgcagttac gtgcgtcatc gcgccagaag agtgcgtttc ggttcatgcg ctgactgtcg
     3121 tggaacttcc ggtgccgttg tggcggtctt ggggtctgtc taggattcgc cgttcaacgt
     3181 tggccaggtt tgggtccaat ggtgtccgcc gtcggtggtg cgccacagtg atccggcggc
     3241 ggagatgatt gcggtgtcgg ggccggtggc ttgtagtggt tggtagagct gttgcacgcc
     3301 gccggggttg gtggtgtagg tcgtccaggt cgtgccggcg tcgctgctgt gcgcgacgct
     3361 ggctgggcag tcgagccaga tctctttgtc gaacaccgaa gccgtgcagc cggtggcgag
     3421 gttgggcgcg gtgatggtgg accaggtggt gccgtcgttg gagctgattt gaaggtgggt
     3481 gcacgcgtcg atcgcgacga gaacgctgga gctggtggcg agcgatccgg gcgcgaacgc
     3541 ggagaacagg aacggcagcg aagctgtcgg tgcgcggttc cagtgcgcac cggcgtcggt
     3601 gctgaggtag agcgtcgcgg gtcgagtttc tggttgccag cccggtttgg actgttcggt
     3661 ctcggcgatg agttgcgggc ttaggcagtc gtcgttgtag ccgatgatcg cggcggctcg
     3721 cccgtcctgg ccgagcgcga ccgcctgcac gctgtcgttc ggggcgacca cgaaactttg
     3781 gtgtgcggtg gttgcgccgt cggtgcttga ccaggtggcc ccggtagacc ggtcaagtgc
     3841 aagcaggtcg cccggtggcg cggcttgcag cgggtcggcg tagctcggca acgagctgag
     3901 cgcaggagcg atcgccggtg tccagcgctc cccggtgtcg ttgctggtgt aggtgccgag
     3961 gttggtttgc caccacagct tctcgccggt gccggccacg ggttgactgc ttacctgcgt
     4021 ggcgccggcg tgcagcgtcc agtgctggcc ggtgtcggtg ctgatagcca acgacgctcc
     4081 gttgcgttgt ccgcgcccgc tgaggtacac atcggcacct gccgccgcca tgccgaggcc
     4141 ttcgcgccct gcggcgtgcc agctgtaacc gccgtccgag gttgtgtaca ggttgccacc
     4201 gcacggcccg ttctgcccgt cgatacaacc gccgttcagc gcgtagccat gctgggcatc
     4261 ggtgaacacg agccggacga tctcgttgtc cacgccgtaa ccgctgccgg tgccagggtc
     4321 gagtggtgtg ctccaccgca cctgccaggt gcgcccgccg tcgtcagtgg cttcgacaag
     4381 ctctgagccg ttgtacttgg cgggtgtgcc acccgcggcg aacccatgct cggggtcgac
     4441 gaacgtgacc gcgtacaacg cacggtcctg acatgagctt ggcagcaagg cccagtgcgc
     4501 acctgcgtcg gtgctgcgat acaccgcccc attgcacgaa tccgtgctcg cgcttccggt
     4561 cgcatacatc gtgacgccat caggcagcac atcgatgccg gtgctcaacg gcgcactcag
     4621 cgtgttccag gtgcggccgc cgtcaaccga tcgcagtgtg tcaccgccga agctacccgt
     4681 cacgatgtca ggaagcgcga gctcgaccgc gtgcgcgccg acgccggttg ccgcgatctg
     4741 ctccccgtac ccgaacgaat ccgccgcctg cgtcgccaca ggaacctgag gatcagtcag
     4801 ctgccacgtc acgccgtccg ggctg
//
LOCUS       DATCBJ010000226         3231 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_263102, whole genome shotgun sequence.
ACCESSION   DATCBJ010000226 DATCBJ010000000
VERSION     DATCBJ010000226.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 3231)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3231)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3231
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_263102"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..916)
                     /locus_tag="VII50_04600"
     CDS             complement(<1..916)
                     /locus_tag="VII50_04600"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose/GDP-mannose dehydrogenase family
                     protein"
                     /protein_id="HEY5050157.1"
                     /translation="MALRLSVIGTGYLGATHAACMAELGYEVTGYDVDEAKIDLLRAG
                     RVPFFEPGLPELLTKQVESGRLRFTTDIAEVAEFGDVHFICVGTPQMKDSFAADLRYV
                     HAAVDSLAPLLTRECLVVGKSTVPSGTAAILAARIAELAPGGDAVELAWNPEFLREGF
                     AVKDTLTPERLVIGVESEHGEKVLRDVWAPLTDTGIPLIVTDFATAELVKVAANSFLA
                     TKISFINAMAEICETTGADVTQLAAALALDTRIGGRFLHAGLGFGGGCLPKDIRAFMA
                     RAEELGVDQAVGFLREVDAINQRRRARMV"
     gene            1045..2220
                     /locus_tag="VII50_04605"
     CDS             1045..2220
                     /locus_tag="VII50_04605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009076559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEY5050158.1"
                     /translation="MTAAGGSPAFDVFALPAEYHELRASVRALADARIAPRAAQIDAT
                     AEFPWDVHAALAQSGFNAPHIPESYGGAGADALATAIVVEEVARACASSALIPAVNKL
                     GTLPLLTEGSQRLRDQYLPPVARGEAMFSYALSEPDAGSDAAAMTTRAERDGDEWVLN
                     GTKRWISGAGESLYYTVFAVTDPNAGGRGISAFVVEKADLGLTFGAPERKLGIKGSPT
                     REMYFDGCRIPSWRLIGHEGTGLHIALRTLDHTRVTIAAQAVGIAQGALDAAIDYTSQ
                     REQFGAPVGEFQGVQFMLADMAMRIEAARALTYTAASKSESDAADLSYFGAAAKCFAS
                     DVAMDVTTDAVQLFGGYGYVKDYPVERMMRDAKITQIYEGTNQIQRLVIAREVRKKI"
     gene            2297..2869
                     /locus_tag="VII50_04610"
     CDS             2297..2869
                     /locus_tag="VII50_04610"
                     /inference="COORDINATES: protein motif:HMM:NF012436.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HEY5050159.1"
                     /translation="MPPQKKASTAKAVPAGPFLSDVTALRARARKELDKGPVTAAYGA
                     DLKRVIEVLNTSLATELVCVLRYKAHYFGAKGLNAEPVAAEFLEHAGEEQVHADRLAE
                     RINQLGGTPDMDPDTLMARSQTQYNTPDDLLEMIKEDLIAERIVIQAYTEAIGWLGHH
                     DPTTRRIFEEILAEEEEHADDMLTLLQRNR"
     gene            2925..>3231
                     /locus_tag="VII50_04615"
     CDS             2925..>3231
                     /locus_tag="VII50_04615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HEY5050160.1"
                     /translation="MNDELRGMPTSASRIRLSRIVDALDANLQGNVHGGAIMRLVDEA
                     AGVVASRHSGGRAVTAVVDELQFVVPVHVGDIVTCDAQINWTGRTSCEVGVRVTAQPW
                     "
BASE COUNT          597 a          995 c         1101 g          538 t
ORIGIN      
        1 cgaccatccg cgcacgacga cgctggttga tcgcgtcgac ctcgcgaagg aagccgacgg
       61 cctggtcgac gccgagctct tcggcccggg ccatgaacgc gcggatgtcc ttgggcaggc
      121 acccaccgcc gaagccgaga ccggcatgca agaagcggcc accgatccgg gtatcgagcg
      181 ccagggccgc ggcgagttgg gtgacgtcgg cgcccgtggt ctcgcagatc tcggccatcg
      241 cattgataaa ggagatcttg gtggccagga aggagttggc cgccaccttg accagttcgg
      301 cggtggcgaa gtcggtgacg atcaacggga tgccggtatc ggtgagcggc gcccagacgt
      361 cccggagcac tttctcgcca tgctctgatt cgactccgat caccagccgc tcgggtgtca
      421 acgtgtcctt gaccgcgaag ccctcgcgca ggaactcggg gttccaggcg agctcgacgg
      481 catcgccacc cggtgccagt tcggcgatgc gagcagcaag gatcgccgcg gtgccagagg
      541 gcacggtcga cttgccgacg acgaggcact cacgggtgag cagaggggcg aggctgtcga
      601 ccgcggcgtg gacgtagcgc aggtccgcgg cgaagctgtc cttcatctgc ggcgtgccga
      661 cacagatgaa atggacgtcg ccgaactcgg cgacctcggc gatgtccgtg gtgaagcgca
      721 gccggcccga ttcgacctgc ttggtgagaa gctccggcag gcctggctcg aagaacggca
      781 ccctgccggc gcgcagcaag tcgatcttgg cctcgtcaac gtcgtagccg gtgacctcgt
      841 accccagctc ggccatacac gcggcgtgtg tcgcaccgag atatccggta ccgatgacgc
      901 tgagtcgcaa agccacagtg gtcctttcgg cggcgagaat cgtccgttga acatcgaggt
      961 gcgggtgaag gtcccacgat gttaacccag gtggcgggcg tacttccggc ctacgatcaa
     1021 ggcgtggaga ccgggagcgt tccaatgacg gcagctggcg gcagcccggc attcgacgtt
     1081 tttgctttgc ctgctgagta ccacgaactg cgcgcgagcg tccgggcatt ggccgacgcg
     1141 cggatcgcgc cgagagcagc acaaatcgac gcaacagctg agtttccctg ggatgtgcac
     1201 gcggctttgg ctcagtccgg ctttaatgca cctcacattc ccgagagtta cggcggtgcg
     1261 ggtgccgacg cgctcgcgac cgcaatcgtt gttgaagagg ttgctcgcgc gtgcgcatcg
     1321 tccgcgctaa tccccgctgt gaacaagctg gggacattgc cgctgttgac ggaggggtcg
     1381 cagcgattgc gcgatcagta cctgccgccg gtcgcgcgag gcgaagcgat gttctcgtat
     1441 gcgttgagcg agccggacgc aggcagcgat gccgcggcga tgacgacgcg agcggagcgg
     1501 gacggcgacg agtgggttct caacggtacg aagcggtgga tcagcggtgc cggggaatcc
     1561 ttgtattaca cggttttcgc ggttaccgat ccgaacgccg gcggccgcgg catctcggcg
     1621 ttcgtcgtcg agaaggccga cctggggctg accttcggag cgcccgagcg caagcttggc
     1681 atcaagggtt cgccgacgcg cgagatgtac ttcgatgggt gcaggattcc gtcgtggcga
     1741 ctgatcggtc acgagggcac cgggctgcac atcgcgctgc ggaccctcga ccatacgcgc
     1801 gtgaccattg ctgcgcaggc agttggcatc gcgcaaggcg cgcttgatgc ggcgatcgac
     1861 tacacctcgc agcgcgagca gttcggtgca cccgtcggcg aattccaagg cgtgcagttc
     1921 atgctcgccg acatggcgat gaggatcgag gcggcgaggg cccttacgta tacggccgcc
     1981 tcgaagtcgg aatcagacgc cgcggacctc agctacttcg gtgcggccgc caagtgcttc
     2041 gcctccgacg tcgcgatgga cgtaacgacc gatgccgtcc agctattcgg cggctacggc
     2101 tacgtgaagg actacccagt cgagcgcatg atgcgcgacg cgaagatcac tcagatctat
     2161 gagggcacca accagatcca gcgcctggtg atcgctcgcg aggtgcgtaa gaagatatga
     2221 cgtgggcgcg agggcgggtt taccttccgt gctcggggta tcaagcgacc gactttgaca
     2281 ccaatggagg agttacatgc caccgcagaa gaaggcgtcg accgcaaaag ccgttccagc
     2341 cggtccgttt ctgtcagacg tgacggcact gcgggctcgc gcgcgcaagg agctcgacaa
     2401 ggggccggtc actgcggcct atggtgccga tctcaagcgc gtgatcgagg ttctcaacac
     2461 gtcgcttgcg accgaactcg tgtgcgtgct gcgttacaag gcgcactact tcggcgcgaa
     2521 gggactgaac gcagaaccgg tggcggcgga attcctcgag cacgccggcg aggaacaagt
     2581 tcacgccgac cggctcgcgg agcgcatcaa tcaactcggc ggtacccccg acatggaccc
     2641 tgacaccttg atggcgcggt cgcagaccca gtacaacacg cccgacgacc tgctcgagat
     2701 gatcaaggaa gacctcatcg ccgagcggat cgtcatccag gcctacacag aggccatcgg
     2761 gtggctgggc caccatgatc cgaccacgcg ccggatcttc gaggagatcc ttgccgagga
     2821 ggaggagcac gccgacgaca tgctgacgct gcttcagcgc aaccgctaac gcttgcaaaa
     2881 gcggtcgcgc gtcgggcgcc gcgcgcgcgt gcgatgatca ccgggtgaac gacgaactca
     2941 ggggcatgcc gaccagcgcg tcacgtatca ggctcagccg tatcgtcgac gcgctcgacg
     3001 ccaacctgca gggcaatgtc cacggtggcg cgatcatgcg cctcgtcgat gaggctgccg
     3061 gcgtggttgc gtcgcggcac agtgggggcc gggccgttac tgcagtcgtc gacgaactgc
     3121 agttcgtcgt tccggtgcat gtcggcgaca tcgtgacgtg tgatgcccag atcaactgga
     3181 cagggcgcac gtcatgtgag gtcggtgtac gcgtgacggc acagccgtgg g
//
LOCUS       DATCBJ010000227        11580 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_226420, whole genome shotgun sequence.
ACCESSION   DATCBJ010000227 DATCBJ010000000
VERSION     DATCBJ010000227.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 11580)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11580)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11580
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_226420"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..181)
                     /locus_tag="VII50_04620"
     CDS             complement(<1..181)
                     /locus_tag="VII50_04620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5050161.1"
                     /translation="MGLRKPWRLRDVHPTRRGLILAWFAFAATFGVLRAITWSIHAHV
                     GHFANVTAGSVHLHHY"
     gene            complement(264..2495)
                     /gene="katG"
                     /locus_tag="VII50_04625"
     CDS             complement(264..2495)
                     /gene="katG"
                     /locus_tag="VII50_04625"
                     /EC_number="1.11.1.21"
                     /inference="COORDINATES: protein motif:HMM:NF011635.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catalase/peroxidase HPI"
                     /protein_id="HEY5050162.1"
                     /translation="MSDHHPDAVVREMNEPEAAERECPVASGRRAYPVEGGSNRDWWP
                     NQLNLKILRKHPAAANPMDPDFDYAEAFKTVDLDALRADIEQVMRTSQDWWPADFGHY
                     GPFFIRMAWHSAGTYRISDGRGGAGAGMQRFAPLNSWPDNGNLDKARRLLWPVKKKYG
                     RALSWADLMVFAGNCALESMGFKTFGFAGGRADVWEPDEDVYWGPEETWLGDDRYTGD
                     RELETPLAAVQMGLIYVNPEGPNAHPDPLGSARDIRETFRRMAMNDEETVALIAGGHT
                     FGKTHGAGDPNLYVGPEPERAALEEQGLGWRQSFGSGKGRDVITSGLEVTWTPTPTSW
                     DNSFFETLFGYEWELTTSPAGASQWQPKDGAGANTVPDPQDGSLVRPPTMLTTDLALR
                     VDPVYEQISRRFLAHPEQFAAAFARAWFKLTHRDMGPIHRYLGALVPQETLIWQDPVP
                     AVTHALIGAADIATLKSQLLSSGLSVAQLISTAWASASTFRGSDKRGGANGARIRLEP
                     QSGWEVNEPAELATVLTTLERIQQSFNASGKQVSLADLIVLGGCAAVEQAAKNAGHNI
                     TVPFTPGRTDASQEETDVESFAPLEPTADGFRNYLGKGHRLPAEYLLIDRANLLNLSA
                     PELTVLIGGLRVLGANYGRSTLGVLTSTPQSLTNDFYVNLLDLGTTWKPTAKDPESFE
                     GRDTTGSVKWTGSRVDLLFGSNSELRAIAEVYASDDAQDKFVRDFIAAWDKVMNLDRF
                     DLA"
     gene            complement(2546..2965)
                     /locus_tag="VII50_04630"
     CDS             complement(2546..2965)
                     /locus_tag="VII50_04630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009073531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HEY5050163.1"
                     /translation="MPTRLEVEHLLRGVDLRVTRPRVAVLTAVYKHPHADTDQIMGVV
                     REDLGEVSHQAVYDVLRALTAAGLVRRIEPAGSAARYESRVGDNHHHVVCRLCGAIGD
                     VDCAVGTTPCLTASDDHGFAIDEAEVTYWGLCLACST"
     gene            complement(3044..4738)
                     /locus_tag="VII50_04635"
     CDS             complement(3044..4738)
                     /locus_tag="VII50_04635"
                     /inference="COORDINATES: protein motif:HMM:NF012961.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 9 protein"
                     /protein_id="HEY5050164.1"
                     /translation="MKFLHNHVGYSHADAKLALLQTDAAFSGGRFSVYGVGVAEAVVT
                     GELRGRGGVMKWRDWRYWEADFSAVQQPGSYFLSLDDAVPPLVSATFTIVAEILDGQV
                     VSDLVHYLKSVRCTGVFDIADSSCPVIDGTDRVDVHGGWYDASGDTSKYLSHLSYANF
                     LNPQQIPQVAWNLIESRARLNESDLWMDDRLVDEALHGADFLVRMQNPAGYFYMTVFD
                     RWSKDVDERMICSYATQEGHRFNTYQAGYRQGGGSTIAALARASGLPRDGEFARTRYL
                     AAAEQGFAHLEQHNVEYLDDGTENMIDDYCALLAAAELFAVTGNAAYGDAAHLRVAKL
                     VDRQHADGWFWADDAKTRSYVHAAEGGMPYAVLLRYLEVFAASGAGSQASQLDAAKMA
                     VRRGLTHELEVSAIADGNPFGYPRQYVLVPGGPDRLQFFMAHKNESGYWWQGENARLG
                     SLACAARWGSHVFADDPQFSAALWRYAQAALDWIFGLNPFDTCMMQGHGHNNPRYDLD
                     KWNAPGGVCNGITAGEFDEDDIDFKQAEETDPSQSWRWTEQWIPHGAWLLAALAHK"
     gene            4917..5606
                     /locus_tag="VII50_04640"
     CDS             4917..5606
                     /locus_tag="VII50_04640"
                     /inference="COORDINATES: protein motif:HMM:NF013962.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HEY5050165.1"
                     /translation="MSVADRPGALGALASAIGEAGGDITAVDVVERGPSAAVDDVLVE
                     TPDATSADTILAKLNSLPAIVIEAWQPFTEGDQLRDGLDIVDGLGSTASRALAAITRI
                     APAVLRARWVVIIDQVNSGVAITQASAGAPWVRWSALPWLPLPSAGIVDADPAWLPHD
                     WGGDPHLAAAPVGASQMVLLAVRPTGPRFRPAEIAKLANLAAIASLAAHSTLPSGIAD
                     SSRSQGVATRG"
     gene            complement(5615..6307)
                     /locus_tag="VII50_04645"
     CDS             complement(5615..6307)
                     /locus_tag="VII50_04645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY5050166.1"
                     /translation="MTRVLVVDDEPQILRALSITLKARAYDVVVAVNGRQALSLAAQS
                     SPDIVVLDLGLPDLDGLDVIRGLRGWSKVPIIVLSGRSQGVSKVDALDAGADDYVTKP
                     FNVDELLARLRAVTRRAPTGTEEVQAIIGEVKIELPGRRLTASTSSIAGVADVRLTPT
                     EWQLLEVLIRNPGRLVTQREMLRSIDRADVGDQSHYLRQFMNQLRRKLEPDPARPRYL
                     MTEPGMGYRFQP"
     gene            complement(6327..8888)
                     /locus_tag="VII50_04650"
     CDS             complement(6327..8888)
                     /locus_tag="VII50_04650"
                     /inference="COORDINATES: protein motif:HMM:NF014728.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY5050167.1"
                     /translation="MSGARGTLRIYLGAAPGVGKTFAMLGEGHRRAERGTDVVVGFVE
                     THGRPHTAEAIADLEVISRRIINYRGSDFTEMDVDAILARHPKVALVDELAHTNVPGS
                     RNEKRWQDVEELLAAGITVLSTVNIQHLESLNDVVEKITGVPQRETIPDSIVRSADQV
                     DVVDLSPEALRRRMAHGNIYGPDKVDAALGHYFRIGNLTALRELALLWVADKVDDALQ
                     HYRHEHQIAETWETRERVLVAITGGPEGETLIRRAARIAARSGADLLALHVAPSDGLT
                     GANPAMLAAQRLLVESLGGTYHQVVGDNISATLIDFAHAENVTQLVLGASRRSRLGGL
                     LNGGVGIGSTTMRMSGDLDVHTVMHDQAGRRHTGPSLPRGLTARRRLAGAVLGAIALP
                     LLTLVLANTRSSFNLSSDLLTYLVVVVAVALVGGLYSALATAVVASLLINYYFTPPLY
                     KWTIAEHNNIVAIAAFVIVAATVSLVVELAARRTSQAARASAESETLATLAGSVLRGQ
                     TGVSALLEQVRETFQLISVALLERDFASDSADQPWTVVESVGAQPCMTPAEADTEVPV
                     GERFALAARGRPLAASDLRILGAFAAQAVTAVEQQRLTAVAEAARPLEEADRMRTALL
                     NAVSHDLRTPLASAKAAVTGLRSADVAWTDSERAELLATADESLDRLHRLVDNLLDMS
                     RIQAGALSIRRQPVALHETVPIALDSLGPTAREIELDVPDTLPEVETDPALLERVIAN
                     LTANALRYSPAGSPPRVSASTHANSVELRIADRGPGIPESERARMFTPFQRLGDTDNT
                     TGVGLGLALSRGLMEALGHTLTAEETPGGGLTMVLAMRVSSAVPNDVATLMPAPR"
     gene            complement(8925..9818)
                     /locus_tag="VII50_04655"
     CDS             complement(8925..9818)
                     /locus_tag="VII50_04655"
                     /inference="COORDINATES: protein motif:HMM:NF014699.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit C"
                     /protein_id="HEY5050168.1"
                     /translation="MFNNLPASVRQHLAALRALLVLTVVAGLAYPLVILVVAQFPGLK
                     SHADGSLVKANGTVVGSSLLGQSFTDAKGNPITKYFQTRPSDAGAGYDPTASGGSNLG
                     PESIIDTLPDPSNSKDTGKQSLLSQVCAQSEAVGQLEGVDGQRPYCTSDGIGAVLSVI
                     HDSGLTGPIKSVVAVNQECPAKPFIATYDGVAVTCATFGVDYRIGKIVLVHGPGKGTD
                     NPVPPDAVTHSASGLDPDISAAYANLQAPRVAKARGISLDQVHKLISKYTVGRTWGFI
                     GNPGVDVLQLNIALDKLSGAA"
     gene            complement(9824..>11580)
                     /gene="kdpB"
                     /locus_tag="VII50_04660"
     CDS             complement(9824..>11580)
                     /gene="kdpB"
                     /locus_tag="VII50_04660"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009074345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpB"
                     /protein_id="HEY5050169.1"
                     /translation="ASQLMQGDFVVVEAGETIPGDGDVVEGIASVDESAITGESAPVI
                     RESGGDRSSVTGGTKVLSDRIVVVITQKPGESFIDRMIALVEGASRQKTPNEIALNIL
                     LASLTIIFVLAVATLQPLAIFSKAQQPGIPNTSALTGNGVTGIVLVSLLVCLIPTTIG
                     ALLSAIGIAGMDRLVQRNVLAMSGRAVEAAGDVNTLLLDKTGTITLGNRQASEFLPMP
                     GVSEADLADAAQLSSLTDETPEGRSVVVLAKERYGLRERHPGELTHATFVPFTAQTRM
                     SGADLAEDGGNRQVRKGAASSVVEWIRAAGGSIPPELGPLVDGISAAGGTPLVVAARD
                     GQGARALGVIHLKDVVKQGMRERFDELRAMGIRTVMITGDNPLTARAIAEEAGVDDFL
                     AEATPEDKMALIKKEQAGGKLVAMTGDGTNDAPALAQADVGVAMNTGTSAAKEAGNMV
                     DLDSNPTKLIEIVEIGKQLLITRGALTTFSIANDIAKFFAIIPAMFAAVYPGLDKLNI
                     MRLHSPQSAILSAVIFNALIIVALIPLALRGVRYRPSSASAMLRRNLYFYGLGGIVLP
                     FLGIKAIDLIIQFLPGIR"
BASE COUNT         1956 a         3786 c         3803 g         2035 t
ORIGIN      
        1 ggtagtgatg caagtgcacc gagcccgcag tgacgttagc gaagtgaccg acatgcgcgt
       61 gaatcgacca ggtgatcgcg cgcagcacgc cgaacgtcgc tgcgaacgcg aaccaggcga
      121 ggatcaggcc ccgcctggtc gggtgtacgt cgcgaagtcg ccacggcttt cgcagtccca
      181 tcggcacatc atcgccgcga ccaatcagcg cgaggtttcg acacgcgcgg tcggcacaac
      241 cggcggccgt cctggaggtg gggtcaggcg aggtcgaacc ggtcgaggtt catcactttg
      301 tcccacgcgg caatgaagtc gcggacgaac ttgtcctgcg cgtcatcgct cgcgtatacc
      361 tccgcgatcg cgcgcagctc ggagttcgag ccgaacaaga ggtcgacgcg gctgccggtc
      421 cacttcaccg agcccgtagt gtcgcgtccc tcgaaggact ccgggtcctt ggccgtcggc
      481 ttccatgttg tgccgaggtc gagcaggttc acgtagaagt cgttggtcag cgactgcggc
      541 gtcgaggtga gcacgcccaa cgtggacctg ccgtagttcg cgccgagcac ccgcagtcct
      601 ccaatgagga cggtcagttc cggggcgctc aggttcaaca gattcgcgcg gtcgatcaag
      661 aggtattcgg ctggcagccg atgtcctttg cccaggtagt tacggaaccc gtctgcggtg
      721 ggttcgagcg gggcgaagga ctccacgtcg gtctcctcct gcgacgcgtc cgtgcgtccc
      781 ggggtaaagg ggacggtgat gttgtggccg gcgttcttgg cggcctgctc gacggcggca
      841 cacccgccga gcacgatcag gtcggcgagt gagacctgct ttccgctggc gttgaagctc
      901 tgctgaatcc gctccagtgt ggtcagcacc gtcgcgagct ccgccggctc gttgacctcc
      961 cagccgcttt gcggctcgag ccggatgcgc gccccgttcg ctccgccgcg cttgtcgctg
     1021 ccgcgaaacg tcgaagccga tgcccaggca gtggaaatga gctgggcgac cgacagtccc
     1081 gacgaaagta gctggctctt gagcgtggcg atgtcagcgg caccgataag cgcgtgcgtg
     1141 accgctggca ctgggtcctg ccaaatcagc gtctcctgcg ggaccagcgc gccgaggtag
     1201 cgatggatgg gtcccatgtc gcggtgcgtc agcttgaacc aggccctcgc aaacgcagcc
     1261 gcgaactgct ccgggtgcgc aaggaagcgc cgcgagatct gctcgtacac ggggtcgacc
     1321 cgaagcgcga ggtcggtcgt cagcatcgtc ggggggcgca ccagtgatcc gtcctgcggg
     1381 tcgggcacgg tgttggcacc ggcgccatcc tttggttgcc actggctcgc acctgccggg
     1441 ctcgtggtga gctcccactc gtagccgaaa agcgtctcga agaagctgtt gtcccagctc
     1501 gtcggcgtcg gcgtccaggt gacttcgagc ccgctggtga tcacgtcacg acccttgccg
     1561 cttccgaagc tctgccgcca gcccaggcct tgctcttcga gtgcggcacg ctcgggctcc
     1621 ggcccgacgt acaggttcgg gtcgcccgca ccatgggtct tgccgaacgt gtgcccgccc
     1681 gcgatcagcg cgaccgtctc ctcgtcgttc atggccatgc gccgaaacgt ctcgcggata
     1741 tcacgggccg agccgagggg gtcggggtgc gcgttggggc cttccgggtt cacatagatg
     1801 agccccattt ggacggcggc cagcggcgtc tcgagttctc ggtcaccggt gtagcggtcg
     1861 tcgccgagcc aggtctcctc cggcccccag tacacgtcct cgtccggctc ccaaacgtcc
     1921 gcgcggccgc ccgcgaagcc gaaggtcttg aagcccatcg actccagcgc gcagttaccg
     1981 gcaaagacca tcaggtccgc ccaggacagc gcgcgtccgt acttcttctt gaccggccag
     2041 agcagccggc gtgccttgtc gaggttgccg ttgtcgggcc agctgttgag cggtgcgaag
     2101 cgctgcatgc cggcgcccgc cccgccacgg ccgtcgctga tgcggtaggt gccggcgctg
     2161 tgccacgcca tccggatgaa aaacggcccg tagtggccga agtcggccgg ccaccagtcc
     2221 tgcgaagtcc tcatcacctg ctcgatatca gcccgcagcg cgtccaggtc gacggtcttg
     2281 aaggcctcgg catagtcgaa gtcggggtcc atcgggttgg cagcggccgg atgcttgcgg
     2341 aggatcttca ggttgagttg gttcggccac caatcgcggt tgctcccacc ctcaacgggg
     2401 tacgcgcggc ggcccgatgc gaccgggcac tcccgttccg ccgcctccgg ctcgttcatt
     2461 tcgcggacga cggcgtccgg gtggtggtcc gacatggcag atccttccga gatcaggggc
     2521 atcaggggca gagtcaggag ttgcgttatg tggagcaggc gaggcacagg ccccagtagg
     2581 tgacctcggc ctcgtcgatc gcgaagccgt ggtcgtctga cgcggtgagg catggcgtgg
     2641 tgccgacggc acaatcgacg tcgccgatag caccgcacaa ccggcacacg acgtgatggt
     2701 ggttgtcccc gacccgcgac tcgtagcgag ccgccgaacc ggccggctcg attcggcgca
     2761 ccaaaccggc ggcggtcagg gcgcgcagca cgtcgtacac cgcctgatgg gacacctcgc
     2821 ccagatcctc acggacgaca cccatgatct ggtccgtgtc ggcatgcggg tgcttgtaca
     2881 ccgcagtcag cacggccacc cggggacgcg tgacgcgcag atcaacccca cgcagtaggt
     2941 gctcgacttc caacctcgta ggcatcaccc gagtattgag ccattttctg gaataagtca
     3001 agattagccg ccaaggccgc cggccaacgg ccttgaaggc tcgctacttg tgggcgaggg
     3061 ccgcgaggag ccacgcgccg tgggggatcc actgctcggt ccagcgccag gactgcgacg
     3121 gatcggtctc ctccgcctgc ttgaagtcga tgtcgtcctc gtcgaactcg ccggcagtga
     3181 tgccgttgca aacgccgccc ggtgcattcc acttgtcgag gtcgtaccgc gggttgttgt
     3241 gcccgtggcc ctgcatcatg catgtatcga agggattgag gccgaagatc cagtcgaggg
     3301 cagcctgggc gtagcgccac agcgcggcgc tgaactgggg atcatccgcg aacacgtgcg
     3361 aaccccatcg ggcggcgcag gccagcgatc ccagccgcgc gttctcgcct tgccaccagt
     3421 agccgctctc gttcttgtgt gccatgaaga actgcaaacg atccggaccg cccgggacca
     3481 gcacgtactg ccgcggatag ccgaacgggt tgccgtcagc gatcgcgctg acctcgagct
     3541 cgtgcgtcaa gccgcggcga acggccatct tggcggcatc caactgcgac gcctgcgacc
     3601 cggcacccga cgccgcgaac acctccagat agcgcaggag caccgcgtac ggcatgccgc
     3661 cttcggctgc atgaacgtag ctgcgggtct tcgcgtcatc ggcccagaac cagccgtcgg
     3721 cgtgctgacg atcgacgagc ttcgccaccc gcagatgcgc ggcatcgccg tacgccgcgt
     3781 tacccgtcac ggcaaacagt tcggcggcgg ccagcagtgc acagtaatcg tcgatcatgt
     3841 tttcggtgcc gtcgtcgagg tattcgacgt tgtgctgctc gaggtgcgca aacccttgtt
     3901 cggcagcagc gagataacgc gtgcgggcaa attcaccgtc tcggggcaag ccgctcgcgc
     3961 gagccagcgc cgcgatcgtg ctgccaccgc cttgccggta gcccgcctga taggtgttga
     4021 accggtgacc ctcctgcgtc gcgtaactgc agatcatccg ctcgtcgacg tccttgctcc
     4081 atcgatcgaa caccgtcatg tagaagtagc ctgccggatt ctgcatgcgc accaagaagt
     4141 cggcgccgtg cagggcctcg tcaaccaggc ggtcgtccat ccagaggtcg gactcgttca
     4201 gcctcgctcg actctcgatc aggttccacg cgacctgcgg tatctgctgg ggattgagga
     4261 agttagcgta cgacagatgc gacaggtact tcgacgtgtc acccgacgcg tcataccaac
     4321 caccatggac gtcgacccga tcggtgccgt cgatgactgg acaactgctg tcggcgatgt
     4381 cgaacacgcc cgtgcagcgc accgacttga ggtagtgcac gaggtcggaa acgacctgcc
     4441 cgtcgagtat ctcggcgacg atggtgaacg ttgcgctcac cagcggtggg accgcgtcgt
     4501 cgagcgaaag gaagtagctg ccgggctgct gcaccgccga gaaatcggcc tcccaatagc
     4561 gccagtctcg ccacttcatg acgccaccac gaccacgcag ctcgcccgtc accaccgcct
     4621 cggccacgcc gaccccgtaa acactgaaac gcccgccact aaacgcggcg tccgtttgca
     4681 gcaaggcaag cttcgcgtct gcgtgcgaat agccgacgtg gttgtgcaag aatttcacgt
     4741 gtcctccgca cctttcgtgt cacgggccat catgcacgta tcagcggaca gcactgccaa
     4801 cggacggccg cgaaatccta atgtcatctc cgcgcagcgg cggcgacaca atcgggcgca
     4861 accatagact cgtgcgctcg gagacgggag cctgagtcat gttgtatcta ctgcgggtga
     4921 gcgtcgccga tcggccgggc gccctcggtg cgctggcgag cgcgatcggc gaggccggtg
     4981 gcgacatcac cgcggtggac gtcgtggaac gaggtccgtc cgcagcggtt gacgacgtcc
     5041 tcgtcgaaac cccggacgcc acctccgcgg acaccatcct cgccaagctc aactcgctgc
     5101 ctgcaatcgt gatcgaagcg tggcaaccgt tcaccgaagg cgaccagctg agagatgggc
     5161 tcgacatcgt cgacgggctc ggatcgacgg cgtcccgggc gctggccgcc atcacccgca
     5221 tcgcgccggc cgtgctgcgc gccaggtggg tggtcatcat cgaccaggtc aacagcggtg
     5281 tcgcgatcac ccaggcaagc gccggcgcac catgggttcg ctggtcggcg ctgccgtggc
     5341 tcccgctgcc gagcgcgggc attgtcgacg ccgatcccgc gtggctccct cacgattggg
     5401 gtggcgaccc tcaccttgct gccgcaccgg ttggtgcttc ccagatggtg ctgctcgccg
     5461 tccgaccgac ggggccgcgg ttccgacctg ccgagatcgc gaaactggcg aacctggcag
     5521 cgatcgcctc gcttgccgcg cattcgacgc tgccttcggg tatcgccgac tcaagccgca
     5581 gtcagggcgt tgccactcgc gggtaacgcg cgcctcacgg ctggaagcgg taacccatcc
     5641 cgggttcggt catcaggtag cggggtcgcg caggatcggg ctcgagcttt cgccgcagct
     5701 ggttcatgaa ctgccgcaga tagtggctct ggtcgccgac gtcggcgcgg tcgatcgacc
     5761 gcagcatctc gcgttgcgtg accagccgcc ctgggttgcg gatgaggacc tcgagcagtt
     5821 gccactccgt cggcgtcagc cggacgtcgg caactccggc gatcgatgat gtagacgccg
     5881 tgagccggcg gcccggcagc tcgatcttga cctcaccgat gatggcctgc acctcctcag
     5941 tgcctgtcgg tgcccttcgc gtcacggccc gcaggcgggc aagcagttcg tcgacgttga
     6001 aaggcttcgt gacgtagtcg tcagcgcccg cgtcgagggc gtcgaccttc gacactccct
     6061 ggctgcgacc cgagagcacg atgatcggaa ccttgctcca cccgcgaagc ccgcggatca
     6121 cgtcgaggcc gtcgagatcg ggcagaccga gatccaggac gacgatgtct ggcgaggatt
     6181 gggcggctaa gctcagcgcc tgcctgccgt tcaccgcgac gacgacgtca tacgcgcgcg
     6241 ctttgagcgt gatgctcaga gcgcgcaaga tctgcggctc atcatcgacg accagcaccc
     6301 gagtcattgt ggctgcgcca tgagtgtcat cgcggtgccg gcatcagcgt cgcgacgtcg
     6361 ttcggaaccg ccgagctcac ccgcattgcg agcaccatgg tcagcccgcc gccgggggtc
     6421 tcctcggccg tcagcgtgtg gcccaacgcc tccatcagcc cgcgtgacag cgccagccca
     6481 agccccacgc cggtggtgtt gtcggtgtcg cccagccgct ggaagggcgt gaacatgcgc
     6541 gcacgctcgc tttccggaat gccgggccca cggtcggcga tccgcagctc caccgagttc
     6601 gcgtgcgtac tcgccgacac ccgcggcgga gagcccgcag gggagtagcg caacgcgttc
     6661 gccgtcaggt tcgcgataac gcgctcgagc agggcgggat cggtctcaac ttcgggcagg
     6721 gtgtctggga cgtcgagttc gatctcgcga gccgttgggc ccaggctgtc gagggcaatc
     6781 ggcacggtct catgaagcgc gaccggttgg cggcggattg acagcgctcc ggcctgaatc
     6841 cgactcatgt cgagcaggtt gtcgacaagc cggtgcagcc ggtcaagcga ctcgtcggca
     6901 gtagcgagca gctccgcacg ctcggagtcg gtccaggcaa cgtcggcact acgcaatccg
     6961 gtgaccgccg ccttcgccga cgcaagcggc gttcgcaagt catgactgac cgcgttcagc
     7021 agtgcggtgc gcatcctgtc ggcctcttcg agcggcctgg cggcctccgc gaccgcggtc
     7081 aatcgttgct gctccaccgc agtcacagcc tgagcggcga acgcgccaag aatgcggaga
     7141 tcggacgccg cgagaggccg accgcgcgcc gccagagcga accgctcgcc aactggaacc
     7201 tcagtatcgg cttcggctgg cgtcatacac ggctgtgctc cgacggactc aacgactgtc
     7261 cacggctggt cggccgaatc actcgcgaag tcccgctcga gcaaggcgac cgatatgagc
     7321 tgaaacgtct cccgcacttg ctccagtaac gcagacacgc cggtctggcc gcgcagcacg
     7381 ctgcctgcca aggtcgcaag cgtctctgac tcggcagacg ctctcgcagc ctgcgatgtg
     7441 cggcgggccg ccaactccac gaccaagctc accgtcgcgg ccacgatgac aaaggccgcg
     7501 atggcgacga tgttgttgtg ttcggcgatc gtccacttgt acagcggcgg cgtgaagtag
     7561 tagttgatca ggaggctggc gaccaccgcg gttgcgagtg cggagtacag gccgcctacg
     7621 agggcaaccg caacgacgac gacgagatac gtcagcaggt cgctcgagag attgaagctg
     7681 ctgcgggtat tggcgagcac gagcgttagt agcggcagcg ctatcgcgcc gagcaccgca
     7741 ccggcgagcc gacggcgcgc ggtgaggccg cgaggtagcg acggaccggt gtgccgtcgt
     7801 ccggcttgat cgtgcatgac ggtgtggaca tcaaggtccc ccgacatccg catcgtggtc
     7861 gagccaattc caacgccgcc gttcagcagc ccgccaagcc tggatcgtcg tgaggcaccg
     7921 agaacgagtt gcgtgacgtt ctcggcatga gcaaagtcga tgagagtggc agagatgttg
     7981 tcgccgacga cctggtggta cgtgccgccg agcgactcga ccaacaatcg ttgcgcggcg
     8041 agcatcgcgg ggttggcgcc ggtcagaccg tcggacggcg cgacatgcaa tgcgaggaga
     8101 tcggcgccac tccgcgcggc gatacgcgct gctcggcgga tcagcgtctc gccctcgggg
     8161 ccaccggtta tggcgaccag aacgcgttcc cgcgtttccc acgtctcagc gatctgatgc
     8221 tcgtgccggt agtgctgcag cgcgtcgtcg actttgtcgg ccacccataa cagcgcgagc
     8281 tcgcgcagcg ccgtgaggtt gccgatgcgg aagtagtgac cgagtgcggc gtcgaccttg
     8341 tctgggccgt agatgttgcc gtgcgccatg cgccgacgta gggcttcggg tgacagatcg
     8401 acgacgtcga cctgatcggc agatcggacg atcgagtcgg gaatggtttc gcgttgcggc
     8461 acgccagtga tcttttcgac gacgtcgttc agcgactcaa ggtgctggat gttcaccgtc
     8521 gacaacacgg taatcccagc ggcgagcaac tcctcgacat cctgccaacg cttttcgttg
     8581 cgtgaaccgg ggacattggt gtgagccagt tcgtcgacga gtgcgacctt ggggtggcga
     8641 gcgaggatcg cgtcgacatc catctcggtg aagtcgctgc cgcggtagtt gatgatccgg
     8701 cgcgagatga cctcgaggtc tgcgatggct tcggcagtgt gtggccgacc gtgggtctcg
     8761 acgaaaccga cgacgacgtc ggtgccgcgc tcggctctcc ggtggccctc gccgagcatg
     8821 gcgaaggtct taccgacgcc tggggccgcg ccaagataga tgcgcaacgt gccacgcgca
     8881 cctgacatcg acgccacctc caagctcaag cctaatcttt ttcgctatgc cgctccggac
     8941 agcttgtcga gcgcaatgtt gagttgcagg acgtcgacac cagggttccc gatgaagccc
     9001 catgtcctgc cgacggtgta cttactgatg agcttgtgca cttggtcgag gctgatgcca
     9061 cgcgccttgg cgactcgcgg tgcctgcaga ttcgcatagg ccgccgaaat gtcaggatca
     9121 aggccgctgg cgctgtgggt caccgcgtca ggggggactg ggttgtcggt tcccttgccg
     9181 ggtccgtgga cgagcacgat cttgccgatg cggtagtcaa caccgaaggt ggcgcacgtc
     9241 actgcgacgc cgtcgtacgt ggcgatgaac ggtttggcgg ggcactcctg gttcaccgcg
     9301 acaacgcttt tgattggacc ggtcaatcca ctgtcgtgga tgaccgagag caccgcgccg
     9361 ataccgtcgc tcgtgcagta gggccgttgg ccgtcgactc cttcgagctg gccaacggct
     9421 tcactttggg cgcagacctg cgacagcagg ctctgcttgc cggtgtcctt gctgttgctc
     9481 gggtcgggca gcgtgtcgat gatgctctct ggtccgaggt tgctgccgcc gctggcggtc
     9541 gggtcgtatc cggcgccggc gtccgacggc ctggtttgga agtacttcgt gatcgggttg
     9601 cccttggcgt cagtgaagct ctgtccgagc aacgacgatc cgacgaccgt tccgttggcc
     9661 ttcaccaagg agccgtctgc gtggctcttt agtccgggga actgggcgac gaccaggatc
     9721 accagcgggt aggccagtcc ggcgacgacg gtcaacacca gcagagcgcg cagcgctgcg
     9781 aggtgttgac gaaccgacgc gggaagattg ttgaacatgt cgttcaccgg attccgggca
     9841 ggaactggat gatcaggtcg atggctttga tgccgaggaa cggcagcacg atgccgccta
     9901 agccgtagaa gtacaggttg cgtcgcagca tcgcgctcgc actgctcggg cggtaacgca
     9961 cgcctcgcag cgccagtgga atcaaggcga cgatgatgag cgcgttgaag ataaccgccg
    10021 acaagatcgc cgactgcggt gagtgcagcc gcatgatgtt gagcttgtcg aggcccgggt
    10081 agaccgcagc aaacatggcg ggaatgatgg cgaagaactt cgcaatgtca ttcgcgatcg
    10141 agaacgtcgt cagcgctcca cgagtaatga gcaactgctt gccgatttcg acgatctcaa
    10201 tgagcttggt cgggttggag tcgaggtcga ccatgttgcc ggcctcttta gcagccgacg
    10261 tgccggtatt catcgcgacc ccgacgtcgg cttgggcgag cgctggcgcg tcgttggtgc
    10321 cgtccccggt catcgcgaca agcttcccgc cggcctgctc cttcttgatc aacgccatct
    10381 tgtcttctgg cgtggcctcg gccaggaagt cgtctacgcc ggcctcctcg gcaatcgccc
    10441 tcgcggtaag cgggttgtcg ccggtgatca tcaccgtgcg gatacccatc gcgcgcaact
    10501 cgtcgaaccg ctcgcgcatg ccttgcttga caacgtcctt tagatgaatc acgccgagcg
    10561 cacgagcacc ttgaccgtcg cgcgcggcga caacgagcgg ggtgccccca gcggcggaga
    10621 tgccgtcgac gagcggtcct agctcgggtg gaatcgagcc gcccgccgcg cggatccact
    10681 cgacgaccga gcttgccgcg cccttgcgga cctgacgatt cccaccatct tcggcaaggt
    10741 cggcgccact catacgcgtc tgcgcggtga atgggacgaa ggtcgcgtgc gtcagttcgc
    10801 ccgggtgccg ttcgcgcagc ccgtaccgct ccttcgccag gacgacgaca gagcggccct
    10861 cgggcgtctc atccgtcagg ctcgacagtt gagcggcatc ggcgagatcg gcctcgctca
    10921 cacccggcat tggcaggaac tccgacgcct gccggttgcc gagcgtgatc gttccggtct
    10981 tgtcgagcag caaggtgttc acatcgccgg cggcctcgac ggctcggccc gacatcgcca
    11041 gcacgttgcg ttgcacgagg cggtccatac cggcgatacc gatcgcggac agcaacgcgc
    11101 cgatcgtggt cgggatcaag cagaccagca atgagacaag gacgatgccg gtgaccccgt
    11161 tgcccgtcag cgcgctggta ttcggtatgc cgggctgctg ggccttggag aagatagcga
    11221 gcggctgcag ggtcgccacc gccagcacga agatgatcgt gagcgaggcg agcaggatgt
    11281 tcagggcgat ttcgttcggc gtcttctgcc ggctggcgcc ctcgacgagc gcgatcatgc
    11341 ggtcgatgaa gctctcgccc ggcttttggg taatcacgac cacgatgcgg tcggagagga
    11401 ccttcgtgcc accggttacc gacgaccgat caccacccga ttcgcggatc accggcgccg
    11461 actcgccagt aatggccgac tcgtcgacac tggcgatgcc ttcgacgacg tcgccgtcgc
    11521 caggaatcgt ctcgcctgct tcgaccacga cgaagtcgcc ctgcatgagc tggctcgcca
//
LOCUS       DATCBJ010000228         3864 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_164486, whole genome shotgun sequence.
ACCESSION   DATCBJ010000228 DATCBJ010000000
VERSION     DATCBJ010000228.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 3864)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3864)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3864
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_164486"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..227
                     /locus_tag="VII50_04665"
     CDS             <1..227
                     /locus_tag="VII50_04665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HEY5050170.1"
                     /translation="ALLPNLAAAGYELPRPLPRFGHGVRVDLSSADGRKISVLGCYHP
                     SQQNTFTGKLTEGMLDDVLELAKTLAPIGS"
     gene            230..1654
                     /locus_tag="VII50_04670"
     CDS             230..1654
                     /locus_tag="VII50_04670"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEY5050171.1"
                     /translation="MTEPAKAEPRSEPIGARQRPHILIVGGGYVGMYTALRLSSRLRR
                     DEAMVTVIDPLSYMTYQPFLPEAAAGNLEPRHVVVPLRRVLPHAQILNGRVTRIEHGR
                     RVVTFLPNAGRSVEISYDIIVVAPGSVARTLPIPGLAENGIGFKTVGEAIFLRNHVLS
                     KLDIAASTADVAVRKRALTFVFVGGGYAGVEAMAELEDMARDACRSYDGVEPSDMRWV
                     LVEASDRILPEVSRDMGVYTVNELLRRNIEVRLGTRLNSAERHHIVLSDGDEFEADTL
                     VWTAGVRPNPLVAQTDFPLDEKGRIKGTEYLRIDGVDDAWTAGDSAAIPDLTKPGQFT
                     GPSAQHAVRQARRLGDNIALVLRGGQPAEYRHSYAGSVASLGLHKGVAQVYGVKLRGW
                     PAWFMHRTYHLSRVPTFNRKVRVAADWTLALFFHREIVSLGALQNPRDEFELATKPAI
                     EPQPAAPAKKAAARKSTPAPRRPE"
     gene            1694..1767
                     /locus_tag="VII50_04675"
     tRNA            1694..1767
                     /locus_tag="VII50_04675"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1728..1730,aa:Leu,seq:taa)
     gene            complement(1832..2059)
                     /locus_tag="VII50_04680"
     CDS             complement(1832..2059)
                     /locus_tag="VII50_04680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4287 domain-containing protein"
                     /protein_id="HEY5050172.1"
                     /translation="MTVHHSEDTHRNLVERIPQVTGRGLPEWFHEIEQGPSFLRLDER
                     VNWLTDEHAIPHGYASAIVREHEKRRIAHDL"
     gene            2157..2540
                     /locus_tag="VII50_04685"
     CDS             2157..2540
                     /locus_tag="VII50_04685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5050173.1"
                     /translation="MAAVPASLADDLGRVVKTLRTTPAGRLRARLSGPFHSRADAGRA
                     LARVLAIAAQGIEDADQPAMPLWRELPVLADLAVGDQVRVLAHDLLAVLPTAASEVWT
                     PSGRVRRAELVRDVVSSVAEVAGFL"
     gene            2593..3219
                     /locus_tag="VII50_04690"
     CDS             2593..3219
                     /locus_tag="VII50_04690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5050174.1"
                     /translation="MNRTAFAAVVGAVGAGAFALASCSISIGTMGQGSPPASVAPIAV
                     TSPAVPASPSTRAAGASPSTTAAGSTYLAFVGPLNTTTFALDTEVLGSPAGLTTQSLS
                     AVIVASAASIQTFDDHLRSATWPRTAEPDIATLAATNDVLVVLLQGYESDLRSPTPGA
                     DFNTVLQALQVENSAYAIDRGLASDDSGRVRSDLGLPPPSAPQVALND"
     gene            3223..3789
                     /locus_tag="VII50_04695"
     CDS             3223..3789
                     /locus_tag="VII50_04695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5050175.1"
                     /translation="MPLKRRYRWPLAVVLFWTFRTGLGLGAFGVIFILVGTPSAFLFS
                     LLMMVVGAAMMLGSASMWARANGRRRGVDHRGDTVRLWRPTGPEELALIEASGWRGWP
                     PRLPDQPIFYPVLSREYAATIARDWNVKRSGVGYVTTFQVRQQFLDDYEIQRVGGREI
                     REYWIPAEDLGELNRNIVGLIEVVAEYR"
BASE COUNT          605 a         1228 c         1320 g          711 t
ORIGIN      
        1 aggcgctgct gcccaacctc gctgccgccg ggtacgagct gccacgcccg ctgccgcgct
       61 tcggccacgg tgtgcgcgtg gatctctcga gcgccgatgg gcggaagatc tcggtcctcg
      121 gctgctacca cccgagccag cagaacacct tcaccgggaa gctcaccgag ggcatgctcg
      181 acgacgtgct ggaactggcg aaaacgctgg ccccgatagg ctcgtaggca tgaccgaacc
      241 ggcgaaagcc gagccgcgga gcgagcccat tggcgcgcgt caaagaccgc acatcctgat
      301 cgtcggcggc ggctacgtcg ggatgtacac ggcgctgcgc ctgagcagcc gcctgcgccg
      361 cgatgaggcg atggtcacgg tcatcgatcc gctgtcgtac atgacctacc agccgttcct
      421 gcccgaggcc gcggccggca accttgagcc ccggcacgtc gtcgtcccgc tgcgccgtgt
      481 cttgccgcat gcacagatcc tcaatgggcg ggtcacgcgt atcgagcatg gacgtcgagt
      541 ggtgacgttt ttgccgaatg cgggccgatc ggtcgagatc tcttacgaca tcatcgtggt
      601 ggcgccgggt tcggtggcgc gcacgctgcc gattccgggt ctcgccgaga acggcatcgg
      661 attcaagacg gtcggcgaag cgatcttctt gcgcaaccat gtgttgtcaa agctcgacat
      721 cgccgcgtcg acggcggacg ttgccgtgcg caagcgcgcg ctcaccttcg ttttcgtcgg
      781 cggcggttac gcgggcgttg aggcgatggc cgaactcgaa gacatggccc gcgatgcatg
      841 caggtcctac gacggcgtcg aaccgtcgga catgcggtgg gtgctcgtcg aggcgagcga
      901 tcgaatcttg ccggaggtca gtcgcgacat gggcgtgtac accgtcaacg aactgcttcg
      961 ccgcaacatc gaggtgcggc tcggtacccg gctgaactcg gccgagcggc atcacatcgt
     1021 gctgtcggac ggcgacgagt tcgaggccga cacattggtc tggactgccg gcgtgcgacc
     1081 caatccgctg gtggcccaaa ccgacttccc gctcgacgag aagggccgca tcaaaggcac
     1141 cgagtatctg cggatcgacg gtgtcgacga tgcgtggacg gcgggtgaca gcgcggcgat
     1201 tccggaccta acgaagccgg ggcagttcac cggtccgagc gcacagcacg cagttcggca
     1261 agctcggcgg ctcggcgaca acatcgcctt ggtattgcgt gggggccaac ccgctgagta
     1321 ccggcactcg tatgcaggct cggtggcgag cctcggattg cacaagggtg tcgcgcaggt
     1381 ctacggcgtc aagctgcgtg ggtggccggc gtggttcatg caccgcacgt accacctctc
     1441 gcgggtgccg acgttcaatc gcaaggtgcg agtcgccgcc gactggacgc tcgcgttgtt
     1501 cttccaccgc gagatcgtgt cgctgggcgc gttgcaaaat ccgcgcgacg agttcgagct
     1561 cgcgacgaag ccggcgattg agccgcagcc cgcggcgccg gcgaagaagg cggccgctcg
     1621 caagagcacg ccagcacctc ggcgtccgga atagctctgc tgtcctgaaa gacttgccgt
     1681 ccggacaggt gtggcccccg tagcccaaca ggcagaggcg aaccccttaa aagggttgca
     1741 gtgtcggttc gactccgacc gggggcaccg gctcacccgt cacggttcgt tacccgggcc
     1801 gggctgctca agcggcggcg atcggccggt tctagaggtc gtgcgcgatg cggcgcttct
     1861 cgtgctcgcg cacgatcgcg ctcgcgtagc cgtgcgggat ggcgtgctcg tcggtgagcc
     1921 aattgacccg ctcatcgagt cgcagaaagc tcgggccctg ctcgatctcg tggaaccact
     1981 cgggaagccc gcggccggta acctgcggga tcctttcgac gaggttgcga tgggtgtcct
     2041 cggagtggtg cacggtcatg agcgcctcca gggcgagccg aactctcagt ggtccatgag
     2101 ttcagggtgc cgcagcgatg gctacgaggg caagcctttc ggataacgtg cgggccgtgg
     2161 ccgccgtccc cgcttcgctc gccgatgatc tcggccgggt cgtcaagacc ctgcgtacca
     2221 cccccgcggg acgcctgcgc gcgcgcttgt cggggccgtt tcacagccgg gcggacgccg
     2281 gaagggcgct ggcccgcgtt ctcgcgatcg ccgcccaggg catcgaggac gccgaccagc
     2341 cggcgatgcc gctttggcgc gaactgccgg tcctcgccga tcttgcggtc ggggatcagg
     2401 tgcgggtcct cgcgcacgac ttgcttgcgg tgttgccgac cgcggccagc gaggtctgga
     2461 cgccgtcggg ccgggtgcga cgagcggagt tggtgcgcga cgtcgtcagc tcagtggctg
     2521 aggttgctgg attcctctga atcgcgagac gttcgcgggt agccttcagt gaacgtggcg
     2581 atgggggaat tcatgaatcg caccgccttt gcggcggttg ttggtgcggt gggtgctggt
     2641 gccttcgcgc tcgcgagctg ctcgatctcg attggcacga tgggccaggg atcgccgcct
     2701 gcgtctgttg cgccgatcgc ggtcaccagc ccggccgtgc ccgcgtcgcc ctctaccagg
     2761 gcggctgggg cgtcgccttc cactacggcg gctgggtcga cctacttggc gttcgttgga
     2821 cccctgaaca ccacaacatt tgctttggac actgaggttc tcggttcgcc ggcgggcctc
     2881 acgactcagt cgctttctgc ggtcatcgtc gcgtctgcgg cgtcgatcca aacattcgac
     2941 gaccacctgc gaagcgcgac ctggccaaga accgcagaac ccgacatcgc gactctcgcg
     3001 gccacgaacg acgtccttgt cgtgcttctg cagggttatg aatctgacct tcgatcacca
     3061 acgccgggcg ccgacttcaa caccgtcttg caggcgttgc aggtagaaaa ttctgcgtac
     3121 gccatcgacc gaggcctggc atcagacgac tctggtcgag ttcggtccga cctcgggcta
     3181 ccgccgccgt ccgcgccgca ggtcgctctc aacgactagc ggatgccact caagcgccga
     3241 taccggtggc cattggctgt cgtgcttttc tggaccttca ggaccggtct cgggctcggt
     3301 gcgttcggcg tcattttcat tcttgtcggc accccgtccg cgtttttgtt ttcgctgctc
     3361 atgatggttg tcggtgcagc catgatgctc ggctccgcct cgatgtgggc tcgggccaat
     3421 ggtcgaagac gcggtgtgga ccatcgtggt gacacggttc ggctgtggcg accgacgggg
     3481 ccggaggagt tggcgttgat cgaagcgtcg ggatggcgtg ggtggccgcc aaggcttccc
     3541 gaccagccga tcttctatcc cgtgctcagc cgggagtacg cggcgaccat cgcgcgggac
     3601 tggaatgtga agcgttccgg tgttggctac gtgacgacgt ttcaggtccg gcagcaattc
     3661 ctcgacgact acgagatcca gcgggtaggt ggtcgcgaga ttcgtgaata ctggattccg
     3721 gccgaggacc tgggcgagtt gaaccgaaac atcgtcggtc tgatcgaagt cgtcgccgag
     3781 taccggtgag cgcgggtgcg cgcgggttac agcggcggca gcacgacgcc gaatccggct
     3841 actgctaggc gcaggagcac ttgc
//
LOCUS       DATCBJ010000229         3773 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_42198, whole genome shotgun sequence.
ACCESSION   DATCBJ010000229 DATCBJ010000000
VERSION     DATCBJ010000229.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 3773)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3773)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3773
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_42198"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..3073)
                     /locus_tag="VII50_04700"
     CDS             complement(<1..3073)
                     /locus_tag="VII50_04700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004352 - glutamate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0019551 - glutamate catabolic process to
                     2-oxoglutarate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-glutamate dehydrogenase domain-containing
                     protein"
                     /protein_id="HEY5050176.1"
                     /translation="MSADGVDGAGDADFLARYFLHVAAEDLADLSAAEQRDVAFGHRE
                     FASIRPSGVPKVRVFASPTQPRHAVIEIVTDDMPFLVDSVTQELARTDVAIHLVVHPQ
                     FVVRRNLHGQLLAVSGALDAHEAPTDALVESWMHLEVFGPPDQNAQESLRRDIVRVLQ
                     DVRDAVEDWPKMRVYALRAADELHVEPPMAVPAAEAGEVVEFLRWLADDHFTFLGYRE
                     YRLIDDDGVEALAAVSGTGLGILRADKLRPRPLADLPQEVRARVRDPQLLLITKANSR
                     STVHRGVYLDYIGIKTFDKSGVVIGERRFLGLFTSVAYTQSVWRIPLLRSKSSAVLEL
                     SGFTPRSHSGKDLIQIIEDHPRDEMFQISVTELYDTAMGVLHLQERRRLRLFMRRDVY
                     GRFVSCLVYLPRERYDTAVRLRMRAILLEALGGSSIDDTFRVAESVLARVHFVVRVDG
                     SSPVEVDRDELESRLVVATRTWEDDFVDALAARFGDELVASVAARYADAFPEAYKEDF
                     AATVAVDDLQRLDALRDDGDLDVELYEPLDTEACDLAVKLFRRGAPVSLSYMLPILQS
                     MGVEVTDERPYEIERRDGTVAWIYDFGLRHGPVGDEAAPETLDLRFQDALAAAWHGRA
                     EVDGFQALVLEAGLTWRQVTVIRAYAKYLRQAGYRFSQAYLESALRANTRVVRLLVSL
                     FEARFDPAFGSDRTGVEMAMVQDLEEALDAVVSLDEDRILRSFLLLILATVRTNHWQL
                     GAEGIPKDYLSLKIESRSVTDLPEPRPWFEIWVYSPRTEGVHLRFGPIARGGIRYSDR
                     REDFRTEVLGLAKTQTVKNAVIVPVGSKGGFVVKRPPAELTDRKAQQAEVVACYRTLI
                     SGLLDLTDNLIAHADGQQTVPPKDTVRHDGDDSYLVVAADKGTATFSDLANGISADYG
                     FWLGDAFASGGSAGYDHKAMGITARGAWESVMRHFRELGVNCQTTDFTCVGIGDMSGD
                     VFGNGMLLSPHIRLLAAFDHRHIFVDPNPDAAVSFAERQRMFALAGSSWADY"
     gene            complement(3070..>3773)
                     /locus_tag="VII50_04705"
     CDS             complement(3070..>3773)
                     /locus_tag="VII50_04705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEY5050177.1"
                     /translation="LVRGAFEYQGQKCSAASRAYVARSVWRRLEGSLLAEVEALPMGD
                     VSDFSNFMGAVIDDRSFARLHEAIKRVQATAGLSVIAGGQTEDSEGWFVRPTVVLGDD
                     PADEVFVREYFGPLLAVHVFDDERYVDVVAQAAVASPYALTGAIIAQDRAAIATATEA
                     LRFSAGNFYVNDKPTGAVVGQQPFGGGRASGTNDKAGSAQNLLRWTSTRAIKETFVPA
                     TSWRYPHMDPAEEDG"
BASE COUNT          715 a         1249 c         1183 g          626 t
ORIGIN      
        1 tgtagtcggc ccatgaactc ccggcaagtg cgaacatccg ctggcgttcg gcgaacgaaa
       61 cggccgcgtc aggattcggg tcgacgaaga tgtgtcggtg atcgaacgct gcgagtaacc
      121 ggatgtgcgg tgagagcagc atgccgttgc cgaacacgtc gcccgacata tcaccgatgc
      181 cgacgcaggt gaaatcggtg gtctggcagt tgactccaag ttcgcggaaa tgccgcatca
      241 ccgactccca cgcgccgcgt gcggtgatgc ccatggcctt gtggtcgtag cccgcggacc
      301 cgcccgaggc gaacgcgtcg ccgagccaga agccatagtc ggcggagata ccgttcgcaa
      361 ggtcggaaaa cgtggcggtg cccttgtcgg ctgccaccac gagatagctg tcgtcgccat
      421 cgtgacggac ggtgtctttc ggcggcacgg tctgctgtcc gtccgcgtgg gcaatgaggt
      481 tgtcggtgag gtcgagaagt ccgctgatca gcgtgcggta gcacgcgacg acctcggcct
      541 gctgcgcctt ccgatcggtc agctcagcgg gaggccgctt gacgacgaaa ccgcccttgg
      601 agccgaccgg cacaatcacg gcgttcttca cggtctgcgt cttcgcaagc ccgagcacct
      661 cggtgcggaa gtcttcgcgc cggtcggagt atctgatgcc accgcgcgca atgggaccga
      721 accgaagatg cacaccctca gtgcgcggcg aatacaccca gatctcgaac cacggccgcg
      781 gctcgggcag gtcggtcacc gaccgcgact cgatctttag cgagaggtag tccttgggga
      841 tgccttcggc gccgagctgc cagtggttcg tgcgaaccgt cgcgaggatc aacaacagga
      901 agctgcgcag gatgcggtct tcgtcgagac tgacgacggc atcaagcgcc tcctcgaggt
      961 cctggaccat cgccatctcg acgccggtcc ggtcgctgcc gaacgccggg tcgaaccggg
     1021 cctcgaacaa cgacaccagc aaccgcacga cccgggtatt ggcccgtagt gcggactcga
     1081 gataggcctg gctgaatcga tagccggcct ggcgcagata ttttgcgtac gcccggatca
     1141 ccgtcacctg ccgccaggtc agccccgcct cgagcacgag cgcttggaac ccatcgacct
     1201 cagcacgtcc atgccaggca gcggcaagtg cgtcctggaa acgcaagtcc aaagtctccg
     1261 gcgccgcctc gtcgccgacc ggcccgtgac gaagcccgaa gtcatagatc cacgcgacag
     1321 tgccgtcccg ccgctcgatc tcgtaagggc gctcgtcggt gacctcgacg cccatcgact
     1381 gcaggatcgg cagcatgtac gacagcgata ccggcgcacc gcggcggaac agcttgacag
     1441 cgagatcgca ggcttcggta tcgagaggct cgtacaactc cacgtcgagg tctccgtcat
     1501 cgcgaagcgc atcgagcctt tgcagatcgt cgacggccac tgtcgcggcg aagtcctcct
     1561 tgtatgcctc cgggaacgcg tcggcgtacc gtgccgcaac gctcgccacc agctcgtcgc
     1621 cgaatcgggc ggcgagtgcg tcgacgaaat cgtcttccca cgtccgcgtg gcgacgacga
     1681 ggcggctctc cagttcgtcg cggtcgacct cgaccgggct actgccgtcg acccgaacga
     1741 cgaaatggac gcgagcgagc accgactcgg cgacgcggaa cgtgtcgtcg atgcttgaac
     1801 cgcccagagc ctcgagcaag atggcccgca ttctcagccg caccgcagtg tcgtagcgtt
     1861 cgcgcggcag gtagacgaga cacgaaacga aacgcccgta cacgtcgcgc cgcatgaaca
     1921 gccgcagccg ccgccgttcc tgcagatgca gtacgcccat cgctgtgtcg tacaactcag
     1981 ttaccgagat ttggaacatc tcgtcacgcg ggtgatcctc gatgatctgg atgagatcct
     2041 tgccgctgtg actgcgcgga gtaaatccgg aaagttcgag caccgccgac gacttgcttc
     2101 tgagtagagg gattcgccac acgctctgcg tgtacgccac agacgtgaac aacccgagga
     2161 accggcgttc gccgatcacg acgccggatt tgtcgaacgt cttgataccg atgtagtcca
     2221 ggtagacccc acgatggacg gtcgaccgcg agttggcctt ggtaatcagc aggagttgtg
     2281 ggtcgcgcac ccgggcgcgc acttcctgcg gcaggtccgc gagtggacgc ggccgaagct
     2341 tgtcggctcg cagaatgccg agcccagtac cagaaacagc ggcgagtgcc tcgacaccgt
     2401 cgtcatcgat taaccggtat tcgcggtagc cgaggaacgt gaagtggtcg tcggctaacc
     2461 accgcaagaa ctcgacgacc tctccagcct ccgcagcagg gacggccatt ggcggctcga
     2521 catgcagctc gtccgcagcg cgcaaggcat acacgcgcat cttgggccaa tcctcgacgg
     2581 catcacgcac gtcctgcaat acccgcacga tgtcacgccg cagtgattcc tgcgcgttct
     2641 ggtcgggtgg cccgaatacc tccagatgca tccacgattc gacaagagca tccgttggcg
     2701 cctcgtgcgc gtcgagcgcg cccgacactg ccaggagctg cccgtgcagg ttgcgccgga
     2761 caacgaactg cgggtgcacg accagatgga tcgccacgtc tgttcgggca agctcctggg
     2821 tgaccgaatc aacgaggaat ggcatgtcgt ccgtgacgat ctcgatgacc gcatgccgcg
     2881 gctgcgtcgg cgatgcgaag acccgcacct tcggcacacc ggacggccgg attgaagcga
     2941 actcgcggtg tccgaacgcg acatcacgct gctccgcggc tgacaagtcg gcgaggtctt
     3001 ccgccgcgac gtgcaggaag tacctcgcga ggaagtcggc atcccccgcg ccgtcaacgc
     3061 cgtcggcgct cacccgtctt cctcggccgg gtccatgtgc gggtagcgcc aactcgttgc
     3121 cggcacgaag gtctccttga tggctcgggt gctggtccat cgcagcagat tctgtgccga
     3181 gccggctttg tcgttggtgc cgctggcccg cccgccgccg aacggctgct ggccaacgac
     3241 cgcgccggtc ggcttgtcat tcacatagaa gttgccggcg gaaaaacgca gcgcctccgt
     3301 cgcagtagcg atcgctgccc ggtcctgggc gatgatcgcg ccggtcaggg cgtaagggga
     3361 ggcaacggca gcctgcgcga ccacgtcgac gtagcgctcg tcatcgaaga catggacggc
     3421 aagaagcgga ccgaagtact cgcgcacgaa gacctcatcg gcaggatcgt cgcccagcac
     3481 aacggttggc cggacgaacc atccttcact gtcttcggtc tggcctcccg caatgacgga
     3541 gaggccggcg gtggcttgca cgcgtttgat cgcctcatgc agacgcgcga atgagcggtc
     3601 gtcgatcact gcacccatga agttcgagaa atcgctgacg tcacccatcg gcagcgcctc
     3661 gacttctgcg aggaggctcc cttcgagtcg ccgccacacc gaacgcgcca cgtacgcacg
     3721 cgacgcggct gaacacttct gcccctggta ctcgaaagcc cctcgcacca aag
//
LOCUS       DATCBJ010000230         2524 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_199001, whole genome shotgun sequence.
ACCESSION   DATCBJ010000230 DATCBJ010000000
VERSION     DATCBJ010000230.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2524)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2524)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2524
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_199001"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..610
                     /locus_tag="VII50_04710"
     CDS             <1..610
                     /locus_tag="VII50_04710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009082901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY5050178.1"
                     /translation="RVAELLERFNLTAAAKRPVKTYSGGMQRRLDIAMGLLHRPAALF
                     LDEPTTGLDPQARTELWAEIGRLSAHEQVAVLLTTHYLDEADQLADRLAIVDHGRIVV
                     EGTPEQLKLELNGDTVQVELATADAVPQALVLLSRVAGLRDVASTGASLRARASSGAR
                     AVPSVLSALEDANIAVSSVAVARPSLDDVYLRHVGRSFEVAA"
     gene            607..1452
                     /locus_tag="VII50_04715"
     CDS             607..1452
                     /locus_tag="VII50_04715"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY5050179.1"
                     /translation="MSSALAQIPTSTPHTPQRRGERGQLLQHSVYLTARSLRTLWRQP
                     TFAAMTLIAPIIWLLLFGQLFRSVVHIPGFSTGTGSYLEFITPGVIVMTALFSSGWAG
                     TVYIDDMNRGVMDRLLASPVRRGAMMIGTLAYQAVTTVVQTLIVFGVAYISGARFRGG
                     VVGIVVTIAAAALISVIMASFSNALALLLRQQEALIGISQFIVLPLQFLSSAIMDTRL
                     SPGWVQHVARYNPVDWAVVASRQALSAGTEWGALWPRLGFLAALAATMAWLATRAFRS
                     YQASI"
     gene            1520..2374
                     /locus_tag="VII50_04720"
     CDS             1520..2374
                     /locus_tag="VII50_04720"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HEY5050180.1"
                     /translation="MNFGVRAAGGAAPSLPPAPSDPAPSIEGAGQSASRWRRVLVIEV
                     WIVFAISLGASGLGALLQLIDDLTVKHVALSSHHTTLNGSVTPDRPWLDLAFQLFGIA
                     TGLVPVALVIYLLHRSRESLHTIGVDRSQPGRDVGLGVLLAACVGGAGLALYIGAHAA
                     GANVVVVPTTLGPHWWRIPVLLLSAAQNGIYEEVLVSGYLLHRLRQFGWGDNKALATS
                     ALLRGSYHLYQGFGGALGNFVMGLIFGRVYQRTGRTLPLIIAHTLMDATTFVGYIYLA
                     HKVSWLPT"
BASE COUNT          350 a          810 c          854 g          510 t
ORIGIN      
        1 ccgcgtcgcc gagttgctcg agcggttcaa cctcaccgcg gcggcgaagc ggcctgtcaa
       61 gacgtactcc ggcggcatgc agcgccggct cgacatcgcg atgggcctgc tccaccggcc
      121 cgcagcgctc ttcctcgacg agcccacgac cgggctcgac ccgcaggcgc gaaccgagct
      181 gtgggcggag atcggccggc tgtcggcgca cgagcaggtg gcggttctgc tcaccacgca
      241 ctacctggac gaggctgacc aactcgctga tcggctggcg atcgtcgatc acggacgcat
      301 tgtcgtcgaa ggcacgccgg agcaactcaa gcttgagttg aacggcgaca ccgtgcaggt
      361 tgagctcgcg acagcggatg cggttccgca ggcgctggtg ttgttgtcgc gggttgccgg
      421 tctgcgcgat gtcgcgtcga ctggtgcgag tcttcgggcc cgtgcctcaa gtggggccag
      481 ggcggtgccg tcggttctgt ccgcgctcga agacgccaac atcgcggtgt cgtcggtagc
      541 ggtcgcgcgc ccttccctgg acgatgttta cctgcgtcac gtgggtcgca gcttcgaggt
      601 ggcggcatga gtagtgccct cgcccagatc ccgacatcga cgccgcacac gccgcagcgg
      661 cgaggcgagc gcgggcagtt gttgcagcat tcggtttacc tgaccgcgcg ttcgctgcgc
      721 accctgtggc gacagccgac gttcgccgcg atgacactga tcgcaccgat catctggctg
      781 ttgttgttcg gtcagctttt tcgatctgtg gttcacattc ctgggttttc caccggcacg
      841 ggctcttatc tggagttcat cacgcctggt gtcatcgtca tgacggcgtt gttctccagt
      901 ggatgggcag gcaccgtcta catcgacgac atgaaccgcg gtgtaatgga ccggctgctc
      961 gcatcgccgg tgcgtcgcgg cgccatgatg atcggcacgc tggcctatca agcggtcacg
     1021 accgtcgtgc agacgcttat cgtgttcggt gtcgcgtaca tcagtggagc ccgctttcgc
     1081 ggcggagtgg tcggcatcgt cgtcaccatt gcggcggccg cattgatctc ggtcatcatg
     1141 gcttcgttct ccaacgcact ggcgttgctg cttcgccaac aggaggcgct gatcgggatc
     1201 agtcagttca tcgtgttgcc cttgcagttc ctgtcgtccg cgatcatgga cacccgattg
     1261 tcgcccggtt gggtgcaaca cgtcgcccgc tacaacccgg tcgactgggc agttgtcgcc
     1321 tcccggcagg cgttgtcggc cggtacggag tggggcgccc tctggccgcg gctcggattc
     1381 ctggcggcgc tggcggcgac gatggcttgg cttgcgaccc gggcgtttcg gagctaccaa
     1441 gcgtccatct gaggtgcgtg gccgcctgga cgcagccggc gtttagcgca acttcaggaa
     1501 tcgttgtcat catgacggga tgaacttcgg cgttcgggcg gccggcggcg cggcgccgtc
     1561 gttgccccct gcgcccagcg acccggcgcc gtcgatcgag ggcgccgggc agtcggcgtc
     1621 ccgatggcgt cgcgttcttg tcattgaggt gtggatcgtt ttcgcgatca gcctcggtgc
     1681 aagcggcctc ggcgcactgc tgcagctgat cgatgacctc acggtcaaac acgttgcgct
     1741 gtcgtcgcac cacacgaccc tgaacggttc agtcacccca gatcggccgt ggcttgatct
     1801 cgcgttccag ctgttcggca tcgccaccgg gctggttccg gtcgcgttgg tgatttacct
     1861 tctgcaccgg tcgagggaat cgctgcacac catcggtgtc gatcggtcgc agcccggtcg
     1921 cgacgtcgga ctcggcgtgc tgctcgcggc gtgtgtcggc ggcgcgggtc tcgccctgta
     1981 catcggcgcg cacgcggccg gcgcgaacgt cgtcgtggta ccgacgacgc tcgggccgca
     2041 ctggtggcgg attccggtgc tgttgttgtc ggctgcgcag aacgggatct acgaagaggt
     2101 gctggtcagc ggctatctcc tgcaccggct acggcagttc ggctggggag acaacaaggc
     2161 actcgcgacg agcgcgttgt tgcgcggttc gtatcacctc taccaggggt tcggcggcgc
     2221 gctgggcaac ttcgtcatgg gcctcatctt cggccgggtg tatcagcgca ccgggcgcac
     2281 gttgccgctg atcatcgcgc acacgctgat ggatgcgacg acgttcgtcg gctacatcta
     2341 cctggcgcac aaggtttcct ggctgccaac ctgaacgcgg cttgcgcgct ggttgcggtc
     2401 aggcgttggc ctcgacgagt gcgcgcagcg cgggtccgat ttcggcatag ccgaactcga
     2461 agccggcgtc gaccagtcgt ttcggcagca cccgctggcc gcccacgacg ttgccggcga
     2521 actc
//
LOCUS       DATCBJ010000231         4406 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_487211, whole genome shotgun sequence.
ACCESSION   DATCBJ010000231 DATCBJ010000000
VERSION     DATCBJ010000231.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 4406)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4406)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4406
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_487211"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..3004)
                     /locus_tag="VII50_04725"
     CDS             complement(<1..3004)
                     /locus_tag="VII50_04725"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012789.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HEY5050181.1"
                     /translation="MVPPVLDEAQRRAVEHRGGPVLVLAGPGTGKTTTIVEAVVQRVE
                     SGEITPDQALVLTFSRRAAAELRERIAARLDRTIREPIARTFHSYAFGLLRANAARRG
                     EPAPRLLSGPEQDLVVRELLQGDVEAGSSGWPMRLHPALLTRGFAQELRDFVQRAVER
                     GVTPRELAQLGRRDRRDDWVAAATFAEQYAQVSALREAASYDPAELIRAAAGLLRADP
                     LVLQEIRAAHEFVVVDEYQDTDPAQEELLSLLVGSRDLLAVGDPDQSIYGFRGADLEG
                     IRRFPDRFRAADGSPPTIVALDVSRRSGEQLLAASRRVAQRLSGSPMHRRLRPAAGLD
                     PGTAEVHLFATTSAEASYIAQRLRELHLLREVPWSRMAVLVRGAAPLPALRRSLQSAG
                     VPVAAHAEEQPLVDVPIVYALLRVLALATGRLDLSGPQGVELAQELVTGPLGRADPLA
                     LRRLRQDLRRLELAAGGGRASGALLAQALVTPAELVAIDPHVAAPARRVTDALVAGRT
                     AAANPAATAETVLWAIWQAAAVVARLSSLALSGAPGSAAADRDLDAAVALFDVAARFA
                     DRLPKAGPDVFLDHLLGQQIPGDTLAPQAPSADGVRLMTAHAAKGLEWDVVAVAGVQE
                     GVWPDLRGRGSLLGSQQLVDVLAAGDDSGGTGPLQAVAARLAEERRLFYVAVTRGRLH
                     LLATAVRSDDQQPSRFLDELVPSERDERPLTRVPRGLDLPAVVAELRTVVTSPGSDET
                     EPVGRRRAAAAAQLARLAAAGVPGADPRDWYGLAPLSDDRGLIDDGELVTLSPSRLEA
                     FARCPLRWLLESAGGTSADSTSQGIGTLVHALAQQVADEGVHGDELAVQLQERLASAI
                     KRVDLGSGWFAVKQRERAAEMVRKLAEWLRTNTRVFVAAEQNFEVTIGRAVLKGQVDR
                     LEQDAEGRLFVVDLKTGKHAPTRAEVEAHAQLGAYQVAVERGAFDEVAAGVHTSGGAA
                     LVQVGTGTVKAAINGQLPLSETDDPGWAL"
     gene            complement(3132..3464)
                     /locus_tag="VII50_04730"
     CDS             complement(3132..3464)
                     /locus_tag="VII50_04730"
                     /inference="COORDINATES: protein motif:HMM:NF013222.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HEY5050182.1"
                     /translation="MARSSMGSFDPDAEVPAFAQRVLDLVDAIPPGKVLAYGDIAEYL
                     GEGGPRQVGSVMSTWGGAVPWHRVVHADGTPAPSHDGEVLRLLRADVTPMRDDRVDMR
                     RARWNPDS"
     gene            complement(3475..>4406)
                     /locus_tag="VII50_04735"
     CDS             complement(3475..>4406)
                     /locus_tag="VII50_04735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ThiF family adenylyltransferase"
                     /protein_id="HEY5050183.1"
                     /translation="IIHGQSDIGRSKAQSARDSIHEINPLVKVVLHEERLENENVFEI
                     FSQYDLIVDGTDNFATRYLVNDACVLLGKPYVWGSIYRFDGQASVFWAKNGPCYRCLY
                     PEPPPPGMVPSCAEGGVLGVLCASIGSIQATEAIKLLTGVGESLIGRLMIYDALEMSY
                     RTVKIRKDPECPICGKNPTITELIDYETFCGAVSDEAQLAAQGSTIVPRELKEWIDRG
                     ENIFLVDVREPAEFEIVSIPGAVLIPKGEFLNGSALEQLPQDKRIVLYCKSGMRSAEA
                     LAIVKGAGFADAVHVGSGVVGWVNQVDPSLPSY"
BASE COUNT          752 a         1565 c         1457 g          632 t
ORIGIN      
        1 cgagcgccca gccagggtcg tcggtttctg acagcggcaa ctgaccgttg atggccgcct
       61 tcaccgtgcc ggtgccgacc tgcacaagtg ccgcaccgcc ggaggtgtgc acaccagccg
      121 cgacctcgtc gaacgccccc cgttcaacgg caacctggta ggcgccgagc tgcgcgtggg
      181 cctcgacctc cgcccttgtc ggcgcgtgct tgccggtctt caagtcgaca acgaacaagc
      241 gaccctcggc gtcttgctcg aggcggtcga cctggccctt caacacggcg cggccgatgg
      301 tgacctcgaa gttctgctcg gctgccacga aaacccgggt attcgtgcgc agccactccg
      361 ccagcttccg gaccatctcc gcagcgcgct cgcgttgctt gaccgcgaac cacccggagc
      421 ccagatcgac ccgcttgatc gcgctggcaa gtcgctcctg caactgcacg gcgagctcgt
      481 caccatgcac gccttcgtcg gcgacctgtt gtgcgagcgc atggaccaaa gtgccgatcc
      541 cctgcgatgt cgaatctgca ctcgtgccac ccgccgactc gagcagccag cgcaacggac
      601 aacgagcaaa agcttcgagg cgggacggcg acagcgtcac gagttcgcca tcgtcgatga
      661 gtccacgatc gtcggaaagc ggtgcgagcc cgtaccaatc acgcggatcg gcgcccggga
      721 ccccggccgc cgccagtcgc gcaagctgag cggccgcggc cgctctgcgt cgtccgaccg
      781 gctcggtctc atccgaacct gggctcgtca cgacggtccg caattccgcc accaccgcgg
      841 ggaggtcgag cccgcgcggc accctcgtca gcggtcgctc gtcgcgctcg ctcggcacga
      901 gctcgtcgag gaaacgtgag ggctgctggt cgtcactgcg cacagcggta gccagcaggt
      961 gcaggcggcc ccgcgtgacc gcgacgtaga acagccgccg ttcctcggca agtcgggcgg
     1021 cgaccgcctg caatggaccc gtccccccag agtcgtcgcc ggctgccagg acgtcgacaa
     1081 gttgctgtga tccgagcaac gacccacgcc cacgcaggtc tggccagacg ccttcttgca
     1141 cacccgcgac ggcaacgaca tcccattcaa gacccttggc ggcgtgcgcg gtcatcagcc
     1201 ggacgccgtc cgcgcttggc gcttgcggcg caagcgtgtc gcccggaatc tgctgaccga
     1261 gcagatggtc aaggaacacg tcaggaccgg ctttcggcag ccggtcggcg aatcgggcgg
     1321 ccacgtcgaa cagcgcgacg gccgcgtcga gatcgcggtc ggctgcggcc gacccggggg
     1381 cgccagacag cgcgagggaa ctgagtcgcg ccacgacagc ggcggcctgc cagatcgccc
     1441 acagcacggt ttcggcggtg gccgcggggt tagcggccgc tgtgcgaccc gcgacgagcg
     1501 catccgtaac gcgtcgcgcc ggcgcggcaa catgcgggtc gatcgcgacg agctccgcgg
     1561 gcgtgacgag cgcctgcgcc agcaaggcac ccgatgcacg gccgccaccc gcggcaagct
     1621 cgagccggcg caagtcctgc cgcaaccggc gcagcgccag cgggtcggca cgcccgagcg
     1681 gcccagtgac cagctcttgg gcaagctcga cgccctgcgg gccactcagg tcgaggcgcc
     1741 cggtggcgag cgcgagcacg cgcaagagcg catacacgat cgggacgtcg accagtggct
     1801 gctcttccgc atgcgcggca accggcacgc cggcggactg caacgagcga cgcaacgcgg
     1861 gcagcggtgc agcgccgcgc acgaggacgg ccattcgcga ccacggcacc tcgcgcagca
     1921 aatgcagctc gcgcaatcgc tgggcgatgt aggacgcctc ggcgctcgtc gtcgcgaaca
     1981 ggtggacctc cgccgtgccg gggtcgagcc cggccgccgg ccgcaatcgg cggtgcatgg
     2041 gcgacccgga cagtcgctgc gcgacgcgcc gcgaggcggc caggagctgc tcccccgagc
     2101 gcctgctcac gtcgagcgcg acgatcgtgg gaggcgagcc gtctgcagcc cgaaagcgat
     2161 cgggaaagcg ccggatgcct tcgaggtcgg cgccgcgaaa tccgtagatg gactggtcgg
     2221 gatctccgac ggcaagcagg tctcggctgc cgacgagtag ggagagcaac tcctcttggg
     2281 cggggtcggt gtcttggtac tcgtcgacga ccacgaactc gtgtgctgcc cgaatctctt
     2341 gcaggacaag gggatcggcc ctcaacagcc cagcagcggc ccggatcagc tcagcggggt
     2401 cgtaagaggc ggcctcgcgc agtgcagaca cctgtgcgta ctgctcggcg aacgtcgctg
     2461 ccgctaccca gtcgtctcgc ctgtcgcggc ggccgagctg cgcgagctcg cgcggcgtga
     2521 ctccgcgctc gacggcacgt tggacgaagt cacgcaactc ctgggcaaag ccgcgggtga
     2581 gcaatgcagg atgcaagcgc atcggccacc cgctgctgcc tgcctcgaca tcgccctgca
     2641 gtagttcgcg cacgacgagg tcttgttcgg gaccagacag cagccgcgga gctggctcgc
     2701 cacggcgagc cgcgttcgca cgcagcaagc cgaacgcgta ggagtggaag gtgcgggcaa
     2761 tcggctcacg aatcgtgcga tcgaggcgcg cagcgatgcg ctcacgaagc tccgcggcgg
     2821 cgcggcgact gaaggtcagg acgagcgctt ggtcgggagt gatctcgccc gactcgacgc
     2881 gctgcaccac ggcctcgacg atcgtggtgg tcttgccggt gccaggcccg gcaagcacga
     2941 ggaccgggcc accgcgatgt tcgacagcgc gtcgctgtgc ctcgtcaaga accggcggaa
     3001 ccacgggccc cgctggagct cgcaccactc gcggcacacc cgtcggccga cgcggacgcc
     3061 gaccacccgc cgtcccgctc gtcgaagaca cgaccgcatc acaccacgaa ccgctgacac
     3121 cgcggggagg atcaggagtc ggggttccac ctggctcggc gcatgtcgac gcggtcgtcg
     3181 cgcatcggcg tcacatcagc acgcaacagc cggagcacct caccgtcgtg acttggcgcc
     3241 ggcgtgccat ctgcgtggac cacccggtgc cacgggactg cgccgcccca cgtagacatc
     3301 accgagccga cctggcgcgg gccgccctca ccgaggtact ccgcgatgtc gccgtaggcc
     3361 agcaccttgc ctgggggtat cgcatcgacg aggtcgagta cgcgttgagc gaacgccggc
     3421 acctcggcgt ccgggtcgaa agatcccatg gaagaacggg ccacgaaccg aaaactagta
     3481 cgagggcaag ctcgggtcga cctgattcac ccagccgacg acgccgctgc ccacgtgtac
     3541 ggcatcagcg aatccggcgc ccttgacaat cgccagtgcc tccgccgacc gcatgcccga
     3601 cttgcagtac aagacgatgc gcttgtcttg cggcagctgc tcgagcgccg acccgttgag
     3661 gaactcaccc ttcgggatga gcaccgcgcc cgggatcgag acgatctcga actcggccgg
     3721 ctcgcggacg tcgaccagaa agatgttctc cccgcggtca atccattctt tgagttcacg
     3781 cggcacgatc gtcgacccct gagcggcgag ttgcgcctcg tccgataccg cgccgcagaa
     3841 cgtctcgtag tcgatcagct cggtgatcgt ggggttcttg ccgcagatcg ggcactcggg
     3901 atccttgcgg atcttcaccg tgcggtacga catctccaag gcgtcgtaga tcatcagccg
     3961 gccgatcaac gactcgccga cgcccgtcag caacttgatc gcctccgtcg cctggattga
     4021 tccgatcgac gcgcacagca ctccgagtac gccgccctcg gcgcacgacg gcaccatgcc
     4081 gggcggcggc ggctccgggt agaggcaccg atagcagggc ccgttcttcg cccagaacac
     4141 cgacgcctgc ccgtcgaatc ggtagatcga gccccagacg tacggcttgc cgagcagcac
     4201 gcacgcatcg ttgaccaggt agcgcgtcgc gaagttgtcg gtgccgtcga cgatcaggtc
     4261 gtactggctg aagatctcga acacgttctc gttctcgagc cgctcctcgt gcaacacgac
     4321 cttcacgagc gggttgatct cgtggatcga gtcgcgcgcc gactgggcct tcgagcggcc
     4381 aatatcgctc tggccgtgga tgatct
//
LOCUS       DATCBJ010000232         2636 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_115457, whole genome shotgun sequence.
ACCESSION   DATCBJ010000232 DATCBJ010000000
VERSION     DATCBJ010000232.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2636)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2636)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2636
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_115457"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            275..1390
                     /gene="pdhA"
                     /locus_tag="VII50_04740"
     CDS             275..1390
                     /gene="pdhA"
                     /locus_tag="VII50_04740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011718873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045250 - cytosolic pyruvate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0004739 - pyruvate dehydrogenase
                     (acetyl-transferring) activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HEY5050184.1"
                     /translation="MTADHGARTSERDLVQLLTPEGERLEHPDYPLEISQDELRSLYR
                     DLSLVRRFDTEATALQRQGELGIWASLLGQEAAQIGSGRALLPGDMAFPTYREHGVAW
                     CRGVDPENLLQLFRGVNFGGWDPKEHNFALYTIVIGAQTLHATGYAMGMQRDGSEDAV
                     IAYFGDGASSEGDVNEAFIFAASYNAPVVFFLQNNQWAISVPEERQSRIPLFRRAHGF
                     GFPGVRVDGNDVLACLAVTRKALQYAREGQGPFLVEAFTYRMGAHTTSDDPTRYRLAS
                     ELENWKLRDPIERVKAYLSRNGLADAAFYEAIDAEGAELAARVRAACLSMPEPKPSDI
                     FSQVYAGGSPLVDEERATFDEYMAGYEHAAVTAEVGH"
     gene            1390..2373
                     /locus_tag="VII50_04745"
     CDS             1390..2373
                     /locus_tag="VII50_04745"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011718872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HEY5050185.1"
                     /translation="MAPLAIARALNLGLRKALESDPKVIIMGEDVGKLGGVFRVTDGL
                     QKDFGDDRVMDTPLAESGIIGTAIGLAMRGYRPICEIQFDGFVFPAYDQIVSQLAKLH
                     YRSQGLVPMPVVIRIPFGGGIGAVEHHSESPEALFAHVAGLKVVACSNSADAYTMIQQ
                     AVVSNDPVIFFEPKRRYWEKSEINVDTPLAQAVALHAARVVRPGRHLTLLAYGPMVKT
                     CLDAAKVAADEGHELEVVDVRSLSPIDWPTITDSVRHTGRCVVVHEAQKFLGMGAELA
                     ARITELCFYEMQAPVLRVGGYPIPYPPSRLEEHFLPDLDRVLDTVDRSLAY"
     gene            2391..>2636
                     /locus_tag="VII50_04750"
     CDS             2391..>2636
                     /locus_tag="VII50_04750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009085107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-containing protein"
                     /protein_id="HEY5050186.1"
                     /translation="MASLKQFKLPDVGEGLTEAEIVHWHVKVGDAVAQNQTIVDIETA
                     KALVELPSPFAGVVSELLIPEGTTVEVGTPIIIIDVAP"
BASE COUNT          457 a          893 c          828 g          458 t
ORIGIN      
        1 ctgcccgccc aagccgccgt ccccaccgca ccatcacacg ccgccgccca agcccgcgcc
       61 gccgattcac ccgtgccccc accacccagg ccgctgactg cgaggccgct gactgcgagg
      121 ccgctaactg cgagggcttc cacgtatccc cacgaccggc gttcgatctg cgacgattgc
      181 ccaggtaccc ggtccgatga tgcctaccgg ctgacccggt ccggcgccca tcgacgagtg
      241 atcctcgcgt tggcgagcag gaggagtggc acgcatgact gccgaccacg gtgcacgaac
      301 gagcgaacgc gatcttgtcc agctgctcac tcccgaggga gagcggctcg agcatcccga
      361 ctacccgctc gaaatctcgc aggacgagct gcgcagcctc tatcgcgacc tctcgctggt
      421 acgccgcttc gataccgagg cgaccgcgct tcagcggcag ggtgagctgg gcatctgggc
      481 atcgctactt ggccaggagg cagctcagat cgggtccggc cgggccctgc tccccggcga
      541 tatggcgttc ccgacgtacc gcgagcacgg ggtcgcctgg tgccgcggtg tcgatccgga
      601 gaacctgctt cagctgtttc gcggcgtgaa cttcggaggc tgggacccga aggagcacaa
      661 cttcgcgctc tacacgatcg tgatcggcgc acagaccctg cacgccactg gctacgcgat
      721 ggggatgcag cgcgacggct ccgaggatgc cgtgatcgcc tacttcggtg acggcgcatc
      781 aagcgaaggc gacgtcaacg aggcgttcat ctttgcggcg tcctacaacg cgcccgtcgt
      841 gttctttctg cagaacaacc agtgggccat ctcggtcccc gaagaacgcc agtcgcgcat
      901 tccgctgttc cgtcgcgcgc acggcttcgg cttccccggc gtacgcgtcg acggcaacga
      961 cgtgctcgcc tgcctcgcgg tgacacgcaa ggcgttgcag tacgcgcgcg agggccaagg
     1021 gcccttcctc gtcgaagcgt tcacctaccg catgggtgcg cacacgacgt ctgacgaccc
     1081 gactcgctac cgcctcgcga gcgaactcga gaactggaag ctgcgtgatc cgatcgaacg
     1141 cgtgaaggcc tacctctcgc gcaacggact cgcggacgcc gcgttctacg aggcgatcga
     1201 tgccgaggga gccgaactcg ccgcgcgagt gcgggccgcg tgcttgtcga tgcccgagcc
     1261 gaagcccagc gacattttct cgcaggtcta tgccggcggt tcgccactcg tcgacgaaga
     1321 gcgcgcaacg ttcgacgagt acatggcggg ctacgagcac gcggcggtaa cggccgaggt
     1381 cggtcactga tggcgccgct ggcgattgcg cgtgcgctga acctgggcct gcgcaaggca
     1441 ctggaatcag accccaaggt catcatcatg ggcgaggacg tcgggaagct cggtggcgta
     1501 ttccgggtca ccgacggctt gcagaaggac ttcggcgacg accgcgtgat ggatacgcca
     1561 ctggcggagt ccgggatcat cggcaccgcc atcgggttgg cgatgcgcgg ttaccggccc
     1621 atctgcgaga tccagttcga cggcttcgtg ttccccgcct acgaccagat cgtcagccaa
     1681 ctcgccaagc tgcactaccg gtcgcaaggc ctcgttccga tgccggtcgt catccgaatt
     1741 ccgttcggcg gcggcatcgg cgcagtcgaa caccacagcg aatcgcccga ggcattgttc
     1801 gcccacgtcg cgggtctcaa ggtcgttgct tgttccaact ccgctgacgc ttacacgatg
     1861 attcagcagg cggtcgtcag caacgatccg gtcatctttt tcgagccgaa gcggcggtac
     1921 tgggaaaagt cagaaatcaa cgtcgacacc ccgctcgcgc aagccgtcgc cttgcatgcc
     1981 gcgcgcgtcg tccggccggg tcggcacctg acattgctcg cctacggtcc gatggtgaag
     2041 acctgcctcg acgctgcgaa ggtggccgcc gacgagggtc acgaactcga agttgtcgac
     2101 gtccgctcgc tctcacccat cgactggccg acgatcaccg actcggtgcg ccacactggt
     2161 cgctgcgtcg tcgttcacga ggcgcagaag ttcttaggta tgggcgccga gctggccgct
     2221 cgcatcaccg aactgtgctt ctacgagatg caggcaccgg tactgcgcgt cggcggctac
     2281 ccgatcccgt acccgccgtc gcgtcttgag gagcatttcc tgcccgatct cgatcgcgtt
     2341 ctcgacactg tcgaccgatc actcgcgtac tgacgcaccg gaggtgcgat gtggcatcgc
     2401 tcaagcagtt caaactccct gatgttggcg agggtctgac cgaagccgag atcgtgcact
     2461 ggcacgtcaa ggtcggtgac gcggtggcgc agaatcagac gattgttgat atcgagactg
     2521 cgaaggcgct tgtcgaactg ccgagcccgt tcgccggcgt cgtgagtgag ttgctgatcc
     2581 ccgaaggcac gaccgtcgag gtcgggacgc cgatcatcat catcgatgtc gcgccc
//
LOCUS       DATCBJ010000233         2199 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_271522, whole genome shotgun sequence.
ACCESSION   DATCBJ010000233 DATCBJ010000000
VERSION     DATCBJ010000233.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2199)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2199)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2199
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_271522"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..257)
                     /locus_tag="VII50_04755"
     CDS             complement(<1..257)
                     /locus_tag="VII50_04755"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003905 - alkylbase DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HEY5050187.1"
                     /translation="MTGRVLKRSFFDRPAAVVAPDLLGCVVEHETTAGVVALRLTEVE
                     AYQGALDPGSHAYRGLTPRNSIMFGEPGYVYVYFTYGMHFC"
     gene            complement(310..1770)
                     /gene="argH"
                     /locus_tag="VII50_04760"
     CDS             complement(310..1770)
                     /gene="argH"
                     /locus_tag="VII50_04760"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HEY5050188.1"
                     /translation="MTDSGSVGADPPAHAPLRLWGGRFEGGPAAALAALSVSVHFDWR
                     LAPYDLMSSRAHARVLYRAGLLDDDELRRMVAALDDLDAACQSGAFVPTVADEDVHTA
                     LERGLIERLGPLGGKLRAGRSRNDQIATDLRLYLRAHVRLLVQRLVDLETALVDVADR
                     HKDAPAPGMTHLQHAQPVLFAHHLLAHVQAFARDVDRLRDWDVRAAISPLGAGALAGS
                     SLPLDPVATAHELGFTSSAANSIDAVSDRDFAAEFAFCAAMIGIHLSRIGEEIVLWTS
                     REFRWVELDDSFATGSSIMPQKKNPDVAELARGKTGRLVGNLSAILTMLKALPLAYDR
                     DLQEDKEPVFDSLDTLLVLLPAVTGMIATMRIDTDRLAAAAPEGFALATDVAEDLVRR
                     GVPFREAHEAVGRLVAWCAQHNCDLPDVDDAQLAELSPHLTPGIRSVLSVRGALDARS
                     ARGGTAPERVAEQMTDLRSRIAGQLAWAAPSGASRP"
     gene            complement(1775..>2199)
                     /locus_tag="VII50_04765"
     CDS             complement(1775..>2199)
                     /locus_tag="VII50_04765"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003885157.1"
                     /note="catalyzes the formation of
                     2-N(omega)-(L-arginino)succinate from L-citrulline and
                     L-aspartate in arginine biosynthesis, AMP-forming; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HEY5050189.1"
                     /translation="REVYEAPGAIALITAHQELENVTTERDLARFKKQVEQRWGELVY
                     DGLWFSPLKYALDEFIAFSQQHVTGDVRLTMHGGRAVVTGRRSPASLYDYKLATYDTG
                     DVFDQSLAKGFVELWGLPSKTAARRDLVVKSGSSGTEA"
BASE COUNT          406 a          735 c          725 g          333 t
ORIGIN      
        1 atacagaagt gcatgccgta ggtgaagtac acatacacgt agccaggctc accgaacatg
       61 atcgagttcc gtggcgtaag accccggtac gcatgcgatc ccgggtcgag tgcaccctga
      121 tacgcctcga cttcggtgag gcgcagcgcg acaacacccg ctgtggtctc gtgctcgacg
      181 acgcagccaa ggaggtcggg cgcgacaacc gccgctgggc ggtcgaagaa cgaacgcttc
      241 aggacacgac cggtcacggc gatgccgtcg cagtccgcag tgctgctcgc cgcggcctcg
      301 ggacgacgct cagggccgcg aggcgccgga gggtgccgcc catgccagct gacctgctat
      361 ccgcgagcgc aggtctgtca tctgctcggc aacccgctca ggtgcggtgc cgccacgtgc
      421 cgagcgagcg tcgagcgcac cgcggacaga aagcacggac cggatcccgg gtgtgagatg
      481 tggcgagagt tcagcgagct gcgcgtcgtc gacatcgggc aggtcgcagt tgtgttgagc
      541 acaccacgcg accaagcgcc cgacggcttc gtgtgcttcg cgaaacggca ctccgcgacg
      601 caccaggtcc tcagcaacgt cggtcgctag cgcaaagcct tcgggcgcgg ccgcggcaag
      661 acgatcggta tcgatgcgca tcgtcgcgat catgccggtc actgccggca gcaacacgag
      721 caaggtgtcg agcgagtcga ataccggctc cttgtcttcc tgcagatccc ggtcgtaggc
      781 gagcggaagg gcctttagca tggtgagaat cgccgacaga ttgccgacga ggcggccagt
      841 ctttccgcgc gccagttccg cgacgtccgg attcttcttc tgcggcatga tcgacgaccc
      901 ggtagcgaag ctgtcgtcga gctctaccca gcggaattcc cggctggtcc acagcacaat
      961 ctcttcaccg atgcgcgaca ggtgaatacc gatcatcgcc gcgcagaacg caaactccgc
     1021 cgcgaagtca cggtcgctca cggcatcaat cgaattcgcg gcggacgacg tgaagccgag
     1081 ctcgtgcgca gtcgcgaccg ggtcgagtgg caacgaagat ccagcgaggg cgccggcacc
     1141 caacggagag atcgcggccc gcacgtccca gtcgcgcaat cggtcgacat cgcgcgcgaa
     1201 cgcctggacg tgcgcgagca ggtggtgggc gaacagcacc ggctgagcgt gttgtaggtg
     1261 cgtcatgcca ggcgcaggcg cgtccttgtg ccgatccgct acgtcgacga gcgcagtttc
     1321 gagatccacc aaccgctgca ccagcagccg gacgtgcgcg cgtaggtaga gccgcaggtc
     1381 ggtggcgatc tggtcgttgc ggctccgacc ggcgcgcaac ttcccgccga ggggtcccag
     1441 tcgctcgatg aggccgcgct cgagggcagt gtgcacgtcc tcgtccgcga cggtcgggac
     1501 gaatgcgccc gactggcagg cagcatccag gtcgtcgaga gcggcgacca tccgccgcag
     1561 ctcgtcgtcg tccagcaaac ccgcccggta cagcacgcgg gcatgcgcac gcgagctcat
     1621 caggtcgtac ggagctagcc gccagtcgaa gtgcaccgac accgacagcg cggcgagcgc
     1681 agccgccgga ccgccctcga aacgcccgcc ccacaaccgc agcggcgcat gtgcgggcgg
     1741 gtcggcgccg accgaaccgg aatccgtcat ccggtcaggc ttccgtgccg gacgagccgc
     1801 tcttcacgac caggtcgcgg cgcgcagcgg tcttgctcgg caatccccac aactccacga
     1861 agcccttggc cagcgactga tcgaacacat cgccggtgtc gtaagtggcg agcttgtagt
     1921 cgtagagaga cgcggggctc cgccggccgg tgacaacagc gcgaccaccg tgcatcgtca
     1981 gccgaacatc accagtgacg tgttgctggg agaacgcgat gaactcgtcg agtgcgtact
     2041 tcagcggaga gaaccacaag ccgtcgtaca ccagttcgcc ccaccgctgc tcgacctgct
     2101 tcttgaatct ggccaggtcg cgttcggtgg tgacgttctc gagctcctgg tgggcggtga
     2161 tcagggcgat cgccccaggc gcctcgtaga cttcacggc
//
LOCUS       DATCBJ010000234         3794 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_42024, whole genome shotgun sequence.
ACCESSION   DATCBJ010000234 DATCBJ010000000
VERSION     DATCBJ010000234.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 3794)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3794)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3794
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_42024"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..649
                     /locus_tag="VII50_04770"
     CDS             <1..649
                     /locus_tag="VII50_04770"
                     /EC_number="2.1.1.163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019513916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="demethylmenaquinone methyltransferase"
                     /protein_id="HEY5050190.1"
                     /translation="PRYDLVNDMLSLGQDRIWRKVVARAVGATKGERVLDVAAGTGTS
                     SASFTTAGADCVACDFSLGMLRQGALQRGGADVAGVRFVAGDALALPFADESFDAVTI
                     SFGLRNVHDVDAALRDLRRVTKPGGRLVICEFSSPTWPPFHRLYDSYLGVLPSVVKKV
                     SSNPGSYVYLAESIRAWPAQAELARQIHAAGWTGVAWRNLTGGIVAVHRAKRSAG"
     gene            688..2112
                     /locus_tag="VII50_04775"
     CDS             688..2112
                     /locus_tag="VII50_04775"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="geranylgeranyl reductase family protein"
                     /protein_id="HEY5050191.1"
                     /translation="MNLFTRGLFTSGARRTRFGLGGESVTGKAADGDSATPSSASAVL
                     ADSVPAGTPTTEADVIVVGAGPAGSATAAHLAQAGLAVALLEKSAFPREKVCGDGLTP
                     RAVTQLVKLGIDVSAEAGWLQNKGLRILAGGMRVELPWPELSSFPDYGLVRTRLDFDQ
                     ILARRAQKLGVALHEQTPATQPVIDGGSGRVVGVMARPVGGARDVMFKAPVIVAADGN
                     SSRFSLALGLHRKENRPMGVAYRRYFESPRHDDDWLESWLELWDRYEGKDRLLPGYGW
                     VFGMGDGTSNVGLGILNTSASFRNVDYRQLLKRWTAGMPKEWAFDEEHARGPVRGAAL
                     PMGFNRKPHYDRGVLLVGDAGGMVNPCNGEGIAYAMESGELAAEIIAQALARPAGVAR
                     ERALHGYPKALDDRYGGYYTVGRVFVNLIGKPGVMRLLTRHGMSHPTIMRFTLKMLAN
                     LTDPKGGDAMDRIINGLTRIAPAA"
     gene            2188..2556
                     /locus_tag="VII50_04780"
     CDS             2188..2556
                     /locus_tag="VII50_04780"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017948722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HEY5050192.1"
                     /translation="MSTNASYVPLLALFLLAAGFAVFSILMGSLSGPKRYNRAKYDAY
                     ECGIEPTPQPVGGGKFPVKYYLTAMLFIVFDIEIIFLYPWAVAFQHLGVFGLVEMVLF
                     ILTVFVAYVYVWRRGGLEWD"
     gene            2566..3120
                     /locus_tag="VII50_04785"
     CDS             2566..3120
                     /locus_tag="VII50_04785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006133126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B family
                     protein"
                     /protein_id="HEY5050193.1"
                     /translation="MGLEENLPSGFLLTSVEKLAGTARKNSLWPASFGLACCAIEMMA
                     SGAGRYDLARFGMEVFRASPRQADLMIVAGRVSQKMAPVLRQIYDQMVEPKWVLAMGV
                     CASSGGMFNNYAIVQGVDHVVPVDMYLPGCPPRPEMLMHAILELHERIQVMKLGPNAK
                     KQAQEREIEALEAVPTHEMKGLLR"
     gene            3117..3767
                     /locus_tag="VII50_04790"
     CDS             3117..3767
                     /locus_tag="VII50_04790"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF005856.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HEY5050194.1"
                     /translation="MSDQPVGQPPTAPVLSGAESEVGAVPAQATASAPSTPRPYGGYF
                     DEVADALAQAYPGVAQAVERSIVDRGELTLHVRREKLRDLAQSLRDDQDLRFELLASL
                     SGVHYPTDAGRELHVVYELTSMTHNRRVRLEVAAPDDDPHVPSVVAVWPTADFQERET
                     WDMFGVIFDGHPALTRIMMPDDWPGHPQRKDYPLGGIAVEYKGATIPPPDERRQYA"
BASE COUNT          616 a         1244 c         1266 g          668 t
ORIGIN      
        1 cccgcgctac gaccttgtga acgacatgct gtcgcttgga caggaccgga tctggcgcaa
       61 ggtcgtcgcg cgggcggtcg gcgcaaccaa gggcgagagg gtgctcgacg tcgctgctgg
      121 taccgggacg tcgtccgcgt ccttcaccac tgccggcgcc gactgtgtgg cctgcgactt
      181 ctccctcggc atgctgcgcc aaggcgccct ccagcgcggc ggcgcggacg ttgcgggagt
      241 gcgcttcgtc gccggcgacg cccttgcgct gccgttcgcc gacgagtcct tcgacgcggt
      301 gacgatctcg ttcggcttgc gcaacgttca cgatgtcgat gcggcgttgc gcgacctgcg
      361 gcgggtcacg aagccgggcg ggcggctcgt catctgtgag ttcagctcac cgacctggcc
      421 gccgtttcac cgtctctacg attcctacct gggcgtgctg ccgtccgtgg tgaagaaggt
      481 cagttccaac cccggctcct acgtctatct cgccgagtcg atcagagcct ggccagccca
      541 ggccgagctt gcccggcaaa tccacgcggc gggttggaca ggcgtcgcct ggcgtaacct
      601 cactggcgga atcgttgccg tccatcgggc gaagcggtcg gcggggtagc gtgcccgcgg
      661 acggcatatg cttctccagc cgctctcgtg aacctgttca caaggggact tttcacgagc
      721 ggtgcgcggc gtacccgctt cggcctaggt ggtgaatccg tgacaggcaa ggcagccgat
      781 ggcgactccg cgacgcctag ctcggcgtct gccgtgctgg ccgattccgt tccggccggg
      841 acgccgacca cagaggccga cgtcatcgtc gtaggggccg ggcctgccgg gtctgcgacc
      901 gccgctcacc tcgcccaagc cggactcgcg gtcgcgctgc ttgagaaatc cgcgttccct
      961 cgggagaagg tctgcggcga cggccttacg ccccgggcgg tcacccaact ggtgaagctc
     1021 gggatcgacg tgagcgccga ggcgggttgg ctacagaaca agggactgcg cattctcgct
     1081 ggcggtatgc gcgttgagct gccgtggccc gagctgtcca gtttccccga ctacggactc
     1141 gtgcggaccc ggctcgactt cgaccagatc ctcgcgcgcc gggcccaaaa gctgggtgtc
     1201 gcgctgcacg agcagacccc cgcaacgcag ccggtgatcg acggtggatc ggggcgggtc
     1261 gtcggcgtca tggcgcgccc ggtcggcggt gcgcgcgacg tcatgttcaa ggcgccggtc
     1321 atcgtcgccg ctgatggcaa ctcgagccgc ttctcactcg cgctcggcct gcatcgcaaa
     1381 gagaaccggc ccatgggagt cgcgtatcgc cgctacttcg agtcgccacg ccacgatgac
     1441 gactggctcg agtcgtggct cgagctgtgg gatcgctacg agggcaaaga ccggctgctt
     1501 cccggctacg gctgggtgtt cggtatgggc gacggcacga gcaacgtcgg gctgggcatc
     1561 ttgaatacgt ccgcctcgtt ccgcaacgtc gactaccgcc aactgctcaa gcgttggacg
     1621 gcgggcatgc ccaaggagtg ggccttcgac gaggagcacg cgcggggtcc ggtgcggggc
     1681 gcggcgctgc cgatgggttt caaccgcaag ccgcactacg accgcggagt gctgttggtt
     1741 ggagacgccg gcggcatggt gaatccctgc aatggcgagg ggatcgcgta cgcgatggag
     1801 tcaggcgagc tggcggccga gatcattgcg caggccttgg cgcgcccggc cggagtcgct
     1861 cgcgaacgcg ccttgcacgg ttatccgaag gcactcgacg accgctacgg cggctactac
     1921 acggtcgggc gggtgttcgt gaatctgatt ggcaagcccg gtgtcatgcg actgctgaca
     1981 cggcacggga tgtcgcatcc gacgatcatg cggttcacct taaagatgct cgcgaacctc
     2041 accgatccca agggcggcga cgccatggac cgcatcatca atggactcac ccggatcgcc
     2101 cctgccgcct gacccgcgcg caggacgcgc ttcgcaccca cctagactga cacggcccta
     2161 gtcggcaagc gagagtgagg aaccacggtg agtaccaacg ccagttacgt gcctctcctc
     2221 gcactgttcc tgctcgctgc cgggttcgcc gtgttctcca ttctcatggg cagcctgagt
     2281 gggccgaagc gctacaaccg tgcgaagtac gacgcgtacg aatgcggcat tgagccaacc
     2341 ccgcaaccgg tcggcggcgg caagttcccg gtgaagtact acctgaccgc gatgctgttc
     2401 atcgtcttcg acatcgaaat catcttcttg tacccgtggg cagttgcgtt ccagcacctc
     2461 ggggtattcg ggctcgtcga aatggtgctg ttcatcctca ccgtgttcgt cgcctacgtg
     2521 tacgtgtggc ggcgcggcgg actggaatgg gactgagaac cagacatggg gcttgaagaa
     2581 aatctgccta gcggcttcct gctgaccagc gtcgagaagc ttgccggcac cgcacgtaag
     2641 aactcgctct ggccggcctc cttcggcttg gcctgctgcg cgatcgagat gatggcgtca
     2701 ggcgctggcc gctatgacct tgctcgcttt ggcatggagg tatttcgcgc gtcgccgcgc
     2761 caggccgacc tgatgatcgt ggctggtcgc gtcagccaga agatggcacc cgtattgcgg
     2821 cagatctacg accagatggt cgagcccaag tgggtgctcg cgatgggtgt gtgtgcatcg
     2881 tcgggcggca tgttcaacaa ctacgcgatc gtccagggcg tcgaccacgt cgtgcctgtc
     2941 gacatgtact tacccggctg cccgccacgg ccggagatgc tgatgcacgc catcctcgaa
     3001 ctgcacgagc gcattcaggt catgaagctt ggcccgaacg ccaagaagca agcgcaggag
     3061 cgcgagatcg aggcgctgga ggccgtgccg acccatgaga tgaagggtct gcttcggtga
     3121 gcgatcagcc agtcgggcag ccgccgacgg cgccggtgct cagcggtgcc gaatccgagg
     3181 tcggtgcggt cccggcgcaa gccaccgcgt ccgcgccttc gaccccacgc ccgtacggcg
     3241 ggtacttcga cgaggtcgcc gacgcgctgg cccaggccta cccaggcgtc gcgcaggcgg
     3301 tcgagcgctc gatcgtcgac cgcggtgaac tgacgctgca cgtgcgcagg gaaaagctcc
     3361 gcgatctcgc ccagtcgttg cgcgacgacc aagacctgcg cttcgagctg ctcgcttcgc
     3421 tgtcgggcgt gcactatccg acggacgcag gccgcgaact gcacgttgtt tacgagctga
     3481 cgtccatgac tcacaaccgt cgcgtcaggc tcgaagttgc cgcgccggac gacgacccgc
     3541 acgttccgtc agtcgttgcg gtctggccaa cggctgactt tcaagagcgc gaaacctggg
     3601 acatgttcgg cgtcatcttc gacgggcatc ccgcgctgac gcgcatcatg atgcccgacg
     3661 actggccggg gcatccgcag cgcaaggact acccactcgg tggcatcgcg gtcgagtaca
     3721 agggcgccac gattccgccg ccggatgagc gcaggcagta cgcatgacca cgaccgatcc
     3781 ttacgcaggc gtgc
//
LOCUS       DATCBJ010000235         2113 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_275259, whole genome shotgun sequence.
ACCESSION   DATCBJ010000235 DATCBJ010000000
VERSION     DATCBJ010000235.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2113)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2113)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2113
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_275259"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1510)
                     /locus_tag="VII50_04795"
     CDS             complement(<1..1510)
                     /locus_tag="VII50_04795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03960 family B12-binding radical SAM
                     protein"
                     /protein_id="HEY5050195.1"
                     /translation="MSSNSAESVFARLEPLLMSVSKPVQYVGGELNSLVKDWAACDVR
                     WCLMYPDAYEVGLPNQGVQILYEVLGELDGVLVERTYSVWPDLEALMREHRIPQFTVD
                     AHRPVGAFDILGVSFSTELGYTNMLNALDLAGIPIESADRTHEHPLVIAGGHAAFNPE
                     PIADFIDAAVLGDGEQAVIAITHIVREWKREGSPGGRAEVLDRLARTGNIYVPRFYDV
                     TYRSDGHIDAVTPNRAGVPARVSKHTLMDLDAWPYPKKPLVPLAETVHERFSVEIFRG
                     CTRGCRFCQAGMITRPVRERSIDTIGAMVDAGLRASGFQEVGLLSLSSADHSEIGPLA
                     KQLADRYEGTNTSLSLPSTRVDAFNVDLANEFSRNGRRSGLTFAPEGGSERMRKVINK
                     MVTEQDLIRTVTTAYAQGWRRVKLYFMCGLPTETDDDVLQIADLARKVIQAGRTASGS
                     RDIGCTVSIGGFVPKPHTPFQWAAQLDHATTDSRLLKLRDTIRTDRQYGKAIG"
     gene            complement(1584..>2113)
                     /locus_tag="VII50_04800"
     CDS             complement(1584..>2113)
                     /locus_tag="VII50_04800"
                     /inference="COORDINATES: protein motif:HMM:NF013279.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HEY5050196.1"
                     /translation="HMLSPYQVNRITAYLHPNSPSVLRDSGFNAHQAQIAIGAGGLTG
                     TGLFHGSQINSGAVYAAHTDFIFATAGEELGFLGSALIIVLLSVILWRGLRIASRAPD
                     LFGRIVAGGVVCWLAFETFENIGMNLGIMPVTGIPLQFVSYGGSSLFASMLAVGLLQN
                     VHIQTQAAQNSSGGL"
BASE COUNT          402 a          669 c          669 g          373 t
ORIGIN      
        1 aaccgatcgc tttcccgtac tgacggtcag tgcgaatcgt gtcgcgcagc ttcagcaatc
       61 gcgagtccgt cgtcgcgtgg tcgagctgcg cggcccactg gaacggcgtg tgcggctttg
      121 gcacgaaccc accgatcgac accgtgcagc cgatgtcgcg gctgccactg gccgtccgac
      181 ctgcttggat cacttttcga gcgaggtcag cgatttgcag cacgtcgtcg tcggtctcgg
      241 tcggcaatcc gcacatgaag tacagcttca cccggcgcca accctgggcg taggcggtgg
      301 tcaccgtgcg gatcaagtct tgctcggtca ccatcttgtt gatgaccttg cgcatgcgct
      361 cgctgccgcc ttcgggcgcg aacgtcaggc cggatcggcg gccgttgcgg gagaactcgt
      421 tcgccaagtc gacgttgaac gcgtcgactc tcgtcgacgg caacgacagt gaggtgttgg
      481 ttccctcgta gcggtcagcc agttgcttgg ccagtgggcc gatctcggaa tggtcggcgc
      541 tgctgagcga cagcagccca acctcctgaa atccgctcgc cctcaggccg gcgtcgacca
      601 tcgcaccgat cgtgtcaatc gacctctcgc gcaccggccg ggtaatcatg ccggcctgac
      661 agaaccggca ccccctcgtg cagccgcgaa agatctcgac gctgaaccgc tcgtggacgg
      721 tttctgccag cggtacaagg ggttttttcg ggtatggcca cgcgtcgaga tccatcaggg
      781 tgtgcttcga aacccgcgcg ggaacgcctg ctcgattcgg ggtaacggcg tcgatgtggc
      841 cgtcgctgcg gtaggtcacg tcgtagaagc ggggcacgta gatattgccg gtgcgtgcaa
      901 gccggtccag cacctcggca cgcccaccag gtgaaccctc gcgcttccat tcgcgcacga
      961 tgtgcgtgat ggcaatgacc gcctgctcac cgtcaccaag gaccgcggcg tcgatgaaat
     1021 cagcgatcgg ctcagggttg aaagcggcgt gaccgccggc gatcaccagc gggtgctcgt
     1081 gcgtgcgatc ggccgactcg atcgggatac ctgcgaggtc cagtgcattc agcatgttgg
     1141 tgtagccgag ttcggtcgag aacgagacac cgaggatgtc gaacgcgccg accggccgat
     1201 gtgcatcgac agtgaactgc gggatgcggt gttcacgcat cagggcttcg agatcaggcc
     1261 agacggagta ggtgcgttcg acgagcacgc cgtcaagttc gcccagcacc tcgtacagga
     1321 tttgcacacc ctgattgggc agcccgacct cgtaggcgtc ggggtacatc aggcaccacc
     1381 tgacgtcgca cgcggcccag tccttgacga gcgagttcag ttcaccgccg acgtactgga
     1441 caggcttgct gacactcatt agcagcggct cgagccgggc gaacaccgac tcggcactgt
     1501 tgctcgacac gactgatgga ccttcccggc agaccgcaga ccgacggcgg ggcgaccgcc
     1561 taagtcccta cgaacagttc aggctacagg ccgccgctgg agttctgagc cgcctgcgtc
     1621 tggatgtgca cgttctggag cagcccgaca gccagcatgc tcgcgaacag tgacgacccg
     1681 ccgtaggaga caaactgcag cggaatcccg gtgacgggca tgatcccgag gttcatgccg
     1741 atgttctcga aggtctcgaa cgccagccag cagaccaccc cgccagcgac gatccgcccg
     1801 aatagatcag gggcccggga cgcgatccgc agcccacgcc acaagatcac ggacagcaaa
     1861 acgatgatca gcgccgagcc gaggaaaccg agttcctcac ccgcagtcgc gaagatgaag
     1921 tcggtatgcg cggcgtagac ggcaccactg ttgatttgcg acccgtgaaa aagcccggtg
     1981 cccgtcagcc caccagcgcc gatcgctatt tgtgcttgat gagcgttgaa accggaatcc
     2041 cgcaggacgg acggcgaatt cggatgcaga taggcggtta tccggttgac ctggtacggg
     2101 ctgagcatgt gca
//
LOCUS       DATCBJ010000236         3506 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_262770, whole genome shotgun sequence.
ACCESSION   DATCBJ010000236 DATCBJ010000000
VERSION     DATCBJ010000236.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 3506)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3506)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3506
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_262770"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..631
                     /locus_tag="VII50_04805"
     CDS             <1..631
                     /locus_tag="VII50_04805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5050197.1"
                     /translation="FCPHPPLLRPGIATGIEIETQSLRSACNAAIQRLIDASPDQLLV
                     LGADGPLVDWQGFAPGLIAGDGQPKPLALLVGAWLLSGRTLGSAPIYVAIGPDGEPVT
                     PWPDLPAVTGVLVMGDGSARRSLKGPGYLDVRAEPFDAAVANALADGATQSLADLDLE
                     LASQLLAAGAPAWKAAANLLGFDSSWNADLLYADAPYGVMYTVALWLPA"
     gene            628..1548
                     /gene="miaA"
                     /locus_tag="VII50_04810"
     CDS             628..1548
                     /gene="miaA"
                     /locus_tag="VII50_04810"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HEY5050198.1"
                     /translation="MRPRVVVVLGPTAAGKSQLGIDLAHRLNGEVVNADSMQLYQGMD
                     VGTAKLTHDERQGVVHHLLDIWPVTRTASVADYQTHARAAIDEIAARGCTPIVVGGSG
                     LYIRAVIDDMRFPGTDAVVRANLEAELAIVGAPEMHARLAAIDAATAATMLPTNGRKI
                     VRALEVAAIGGGLFTGLMPSYESVYDAVLIGVDRPTGELDERLTRRVDWMWERRLVEE
                     VRGLAEHSALREGVTARRALGYKQVLAMLDGETSEEEARARTVQATKRFVRRQRSWFR
                     PDPRIGWLIAGRDLTDHALGITELSWRPGL"
     gene            complement(1517..1885)
                     /locus_tag="VII50_04815"
     CDS             complement(1517..1885)
                     /locus_tag="VII50_04815"
                     /inference="COORDINATES: protein motif:HMM:NF024694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase ligase N-terminal
                     domain-containing protein"
                     /protein_id="HEY5050199.1"
                     /translation="MIGDSRPAFVLHEHHKPRHHFDLRLETDGVLRSWALPRGLPPES
                     THDRLAVPVPDHELEHLTYTDADKSIADIGWWEQHDANERRFVFTLHGASSDVRYALI
                     NTGDDWLLHRVKDQGAKTIR"
     gene            1939..2370
                     /locus_tag="VII50_04820"
     CDS             1939..2370
                     /locus_tag="VII50_04820"
                     /inference="COORDINATES: protein motif:HMM:NF016001.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family deazaflavin-dependent
                     oxidoreductase"
                     /protein_id="HEY5050200.1"
                     /translation="MTVVNDWNAKVIADFRANEGRVGGNFEGAPVTILHHVGRKSGQE
                     YLAPVMYLPADGDPDTIYVFASAGGAPQHPKWYVNLTAAGNATVEVGTQSYPVTVTEL
                     TGAERDRVYAEQVKRYPGFGEYEQKNVGIRTIPVLALRKAA"
     gene            complement(2379..2798)
                     /locus_tag="VII50_04825"
     CDS             complement(2379..2798)
                     /locus_tag="VII50_04825"
                     /inference="COORDINATES: protein motif:HMM:NF019656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1707 domain-containing protein"
                     /protein_id="HEY5050201.1"
                     /translation="MSASLRIGDAEREAAISSLTSHFAEGRLTQVEHEERTELALNAR
                     TGRDLDVLFADLPRRGDETGAIRRPSRGPRLEWLVMPLFAALFIGGVIFVILNLLPIF
                     ALVVLAIVASRLLFGARFRRHHWHGGYGSYGGPQRRW"
     gene            complement(2795..>3506)
                     /locus_tag="VII50_04830"
     CDS             complement(2795..>3506)
                     /locus_tag="VII50_04830"
                     /inference="COORDINATES: protein motif:HMM:NF015513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEY5050202.1"
                     /translation="ERAGNLIAGAHKARLHKMMHAHLAAATAAATHGATQQEPWSSAE
                     GPRGDRWPGQHHRGRGPWGFGGPPFPGPWFAGGGRPRPRRGDVRLAVLTLLAEQPMHG
                     YQIITELGSRSGGVWRPSPGSVYPTLQQLQDEGLVTVSEEDGRRTFSLTDAGRAEVER
                     ASAGRRAPWEAMADEADDAAGGLKSLVGQLIAATIQVATAGTHDQVAQAEKVLTDARR
                     AMYRLLAEDEPDAPKADS"
BASE COUNT          567 a         1157 c         1195 g          587 t
ORIGIN      
        1 attctgcccc catccgccgc tcctgcggcc cgggattgcc accggaatcg aaattgagac
       61 gcagtcgttg cgttcggcgt gcaatgcggc tatccagcgg ctgattgatg cgagccccga
      121 ccagctgctt gtgctgggag ccgacggacc cctcgttgac tggcaaggct tcgcccctgg
      181 cctgatcgcc ggcgacggac agccgaagcc gttagcgctg ctggtcggag cctggctgct
      241 gtccggtcga acgctggggt ctgcgccgat ttacgtcgcg attgggccgg acggcgagcc
      301 ggtgaccccg tggccggacc tgcccgcagt caccggcgtc ctagtgatgg gcgacggcag
      361 cgcgcgacgc agccttaagg gtcccgggta tctcgacgtt cgggcggaac cgttcgatgc
      421 cgccgtcgcc aatgcgctcg ccgatggcgc tacgcagtcg ctcgccgacc tcgacctcga
      481 gctcgcgagc cagctgctgg cagctggtgc ccctgcatgg aaggcggcgg cgaatctgct
      541 cggtttcgac agcagttgga acgccgatct tctctatgcc gacgcgccct acggcgtcat
      601 gtacacggtc gctttgtggc tgccggcgtg aggcccagag tcgtcgtcgt cctcggcccg
      661 accgccgcgg gcaagtcgca gctcggcatc gaccttgcgc atcgtctgaa cggcgaggtt
      721 gtgaacgccg actcgatgca gctgtaccag ggcatggacg tcggaacagc gaagctgacg
      781 cacgacgagc ggcagggcgt cgtccatcat ctgcttgata tctggccggt cacgcgcacc
      841 gcgagcgttg ccgattacca gacgcatgcg agggccgcga tcgacgagat tgccgcgcgg
      901 ggttgcacgc cgatcgtcgt gggtggttcc ggtctgtaca ttcgtgcggt catcgacgac
      961 atgcgctttc caggcacgga cgccgttgtg cgggcgaatc tcgaagccga actcgcaatt
     1021 gtcggtgcac cggaaatgca tgcgcggctg gccgcgatcg acgccgcgac cgcagcaacg
     1081 atgttgccga ccaacggccg caagatcgtc cgcgcactcg aggtcgccgc gatcggtggt
     1141 gggttgttca ccggcctgat gccatcgtac gaatcggttt acgacgccgt gctgatcggt
     1201 gtcgaccggc ctactggcga actcgatgag cgactgaccc ggcgggtcga ctggatgtgg
     1261 gagcgccggc tcgtcgagga agtgcgtggc ttggcggagc acagcgcgtt gcgtgagggc
     1321 gtgacggcgc gacgcgccct gggctacaag caggtgcttg cgatgctcga tggcgagacc
     1381 tccgaagagg aggcccgcgc gcgaactgtg caggcaacga agcggttcgt gcgtcggcag
     1441 cgatcatggt ttcgccccga cccgcgcatt gggtggctca tcgcgggacg cgacctgacc
     1501 gaccatgcct tagggatcac cgaattgtct tggcgccctg gtctttgacg cggtgcagaa
     1561 gccagtcgtc gccggtgttg atcagcgcgt agcggacatc cgaactggca ccgtgcagcg
     1621 tgaacacgaa ccttcgttcg ttcgcatcgt gctgctccca ccagccgatg tcggcaatcg
     1681 acttgtcggc gtcggtgtac gtgaggtgct cgagctcgtg gtcgggcacg gggaccgcta
     1741 gccgatcgtg cgtcgactcc ggcggcaggc cgcgggggag cgcccacgac cggagcaccc
     1801 cgtcggtctc gaggcgcaga tcgaagtgat ggcgcggctt gtggtgctcg tgtagcacga
     1861 acgccgggcg ggaatcacca atcatggaga gccgttactg ttgagagcgc aatcaaatct
     1921 gatcgatcgg agaactccat gacagtcgtc aacgattgga acgccaaggt catcgccgac
     1981 ttccgggcaa acgagggccg cgtcgggggc aacttcgagg gcgccccggt cacgatcttg
     2041 catcacgtcg gtcgcaagag cggccaggag tacctcgcgc ccgtcatgta cctgcccgct
     2101 gatggcgacc ctgacacgat ctacgtcttc gcatcagcag gcggcgctcc gcaacacccg
     2161 aagtggtacg tcaacctgac ggcggccggg aacgcaaccg tcgaggtcgg gacgcaaagc
     2221 tacccggtga ccgttacgga actgaccggc gctgaacgcg accgcgtgta cgccgagcag
     2281 gtcaagcgct accccgggtt cggggagtac gagcagaaga acgtcgggat ccgcacgatc
     2341 ccggtgctcg cgctacgcaa ggccgcctga ccgggcggct accaccgacg ctgcggaccg
     2401 ccgtacgagc cgtagccacc gtgccagtgg tgccggcgga accgcgcgcc gaatagcagc
     2461 cggctcgcca cgatcgccaa gacgacgagc gcgaagatcg gcagcaggtt caggatcacg
     2521 aagatcaccc caccgatgaa gagcgccgca aacagtggca tcacgagcca ctccaaacgg
     2581 gggccccgag acggacgccg gatggcgccc gtctcgtcgc cccgtcgggg cagatccgcg
     2641 aacagcacgt cgagatctcg tcccgtccgg gcgttcagcg cgagttctgt tcgctcttcg
     2701 tgctcgacct gggtgagcct gccttcagca aaatggcttg tcagcgagga aatagcggct
     2761 tcgcgttcgg cgtccccgat gcgcaatgac gcgctcatga gtcggccttc ggcgcgtccg
     2821 gttcgtcctc ggcgagcagt cggtacatcg ctcgacgcgc atcggtcaga accttctcgg
     2881 cctgcgcgac ctggtcatgc gtgccggcgg ttgccacttg gatggtcgcc gcgatcaact
     2941 gcccgaccag gctcttgagc ccgccggctg cgtcgtccgc ctcatcggcc atggcctccc
     3001 acggggcccg ccggcccgca gatgcgcgct cgacctcggc cctcccggcg tccgtcagtg
     3061 agaaggtgcg ccggccgtct tcctccgaga ccgtgacaag accttcgtcc tgtaactgct
     3121 gaagcgtggg gtacaccgag ccggggctcg gccgccatac gccgccgctg cgcgatccca
     3181 gctcggtgat gatctggtag ccgtgcatcg gttgctcggc caggagcgtc aacaccgcga
     3241 gccggacgtc gccgcgccgc ggccttggtc ggccgccacc cgcgaaccac ggaccgggaa
     3301 acggcgggcc gccgaagccc catggtccac gaccgcggtg gtgctggccc ggccagcgat
     3361 ctccgcgcgg cccctcggca ctgctccagg gctcttgctg cgtggcgcca tgggtggctg
     3421 cagcggttgc ggcggcgaga tgggcgtgca tcatcttgtg aaggcgggcc ttgtgggctc
     3481 ccgcaatgag gttgccggcg cgctcg
//
LOCUS       DATCBJ010000237         5830 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_347438, whole genome shotgun sequence.
ACCESSION   DATCBJ010000237 DATCBJ010000000
VERSION     DATCBJ010000237.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 5830)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5830)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5830
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_347438"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..450
                     /locus_tag="VII50_04835"
     CDS             <1..450
                     /locus_tag="VII50_04835"
                     /inference="COORDINATES: protein motif:HMM:NF020121.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Beta-galactosidase C-terminal domain"
                     /protein_id="HEY5050203.1"
                     /translation="GGSGRVWTELLTPAGAQVVTTYVDGPLPGSPAITRRDVGAGVAH
                     YVTTVLDAPSTRRLLHGICNDAGVATRSNNNEVGAPIIEAAGIETIRRRAEGASYLFV
                     INHSDREVDVPASGVELLSGTAATGSVHVPPGGVAVVRETTTAGAAG"
     gene            456..1271
                     /locus_tag="VII50_04840"
     CDS             456..1271
                     /locus_tag="VII50_04840"
                     /inference="COORDINATES: protein motif:HMM:NF012669.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HEY5050204.1"
                     /translation="MLAKQRQSMILEHVRRSGGVRVSELTELLGVSDMTVRRDLDVLA
                     RHNLIEKVHGGATVASAPSMDEPGFVAKSSRELSEKEAIAVAAARLVRPGTAIALSGG
                     TTTFALAQRITHVRDLTIVTNSIRVIDVLQQAPGAPTVILTGGIRTPSDALVGPVADL
                     AIRSLHFDIFFLGCHGMDPHAGLTTPNLAESETNRAFIRGARQVVLLADHTKWGTVGL
                     SSFADLDEADVLITDPGLSDDARTTASEHIDRVIITGTGPDAEAGGLATVRQI"
     gene            complement(1268..2173)
                     /locus_tag="VII50_04845"
     CDS             complement(1268..2173)
                     /locus_tag="VII50_04845"
                     /inference="COORDINATES: protein motif:HMM:NF013431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HEY5050205.1"
                     /translation="MPSGAQYRIGHGNQRATVVEVGAAVREYRDGDREIFQSYPERDV
                     SWAFHGSVLLPWPNRLRDGRYEFDGEPHQVPLSEPSRHNALHGLAAWRSWTLVKQEPA
                     YVTLRCQILPSPGYPFRLDTLVNYELGDDGLVVTTTSTNNGDWACPYALGFHPYVSTG
                     AGATLDDCTLHIDASRRLLVDDRLIPVGDEPVAGTADDFRGEQSLAGRVLDTGFTEVS
                     ADAAGHSNVSVACSDGHTVEVWADANFGFWQVYSGDELPVPLARRSLAVEPMTAAPNA
                     FQSGQGLRRLEPGESVTSQWGARLR"
     gene            2407..3597
                     /gene="galT"
                     /locus_tag="VII50_04850"
     CDS             2407..3597
                     /gene="galT"
                     /locus_tag="VII50_04850"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008108 - UDP-glucose:hexose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HEY5050206.1"
                     /translation="MVDAARGHAHRTSTLLADGRELIYFDERATHTDRTRVVDRRVLP
                     TSHPSSTLRLDRILDEWVAIAGHRQTRTYLPPDSECPLCPSSAAYASEIPATDYDVVV
                     FENRFPSFSLGSWVAEEVDRSGANGNGLFATAPGFGRCEVVCFTSDHDAAFSSLTPAR
                     VRTVIDAWVDRTEELSRNPKIEQVFCFENRGEEIGVTLSHPHGQIYGYPYVTPRTRQM
                     LASARAHADATGRNLFADVVTAETQSGERVVVQSSCWTAFVPFSARWPLEVHLYPNRQ
                     VPDLPSLTDEERGDLSHVYLEVLRRMEAVYDDSLPYIAAWHQAPIRVDRDLAYLHLEV
                     FSIRRAPGKLKYLAGSESAMGAFVNDVLPEEAAKLLRSVIPNMTDLTADAAEYGAAEY
                     GHLR"
     gene            3594..4562
                     /gene="galE"
                     /locus_tag="VII50_04855"
     CDS             3594..4562
                     /gene="galE"
                     /locus_tag="VII50_04855"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020657814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HEY5050207.1"
                     /translation="MKLLVTGGAGYIGSVVSRLLLDEGHDVTVLDDLSTGHADAVAEG
                     ADFVETDLTHASDVLARARYDGVLHFAAKSLVGESMVKPSLYWRNNVGGSRDLLDAVT
                     AHGVPRFVFSSTAAVYGEPDEIPIRETARTRPTNAYGATKLAVDLMIRDETMAHGLGA
                     VSLRYFNVAGASRGAGERHATETHLIPIALDVADGLRPALDVYGTDWPTPDGTCIRDY
                     VHVEDLARAHVLALHAAHEGTHLVCNLGNGGGFSVRAVVDTVQVVTRRDVPIRKTARR
                     AGDPAQLVASHDVASAQLGWTPTADLTSIISDAWDFHRSIKKTAQS"
     gene            4559..5701
                     /gene="galK"
                     /locus_tag="VII50_04860"
     CDS             4559..5701
                     /gene="galK"
                     /locus_tag="VII50_04860"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004335 - galactokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HEY5050208.1"
                     /translation="MTATDLFASTFDRPPTGVWSAPGRVNLMGEHTDYNGGYVLPFAI
                     NLRTQAAAARRGDDRVSVQSLQRPGEFIEFALDGLSPQIGSGWASYAIGVLWALRGAG
                     HHIGGLDIVLDGQVPSGAGLSSSAAMECAVGLAAAELYEIDIPGAELARIAQYAENNF
                     VGVPCGLMDQMAACVCQADHALFFDVRDDVREQEPFAPDDEGLAVLVIDTRAHHAHAG
                     GGYAERRKACEEAARQLGVPYLRDIPGSELESALGQLDDELLRRRARHIITENDRVLT
                     TANLLAAGALRNIGPILTESHASMRDDFEISVPELDTAVDAAIAAGALGARMTGGGFG
                     GSAIALIHAAELDEVSRAIEAAFDRLEFTAPQIWSPRASSGASRES"
BASE COUNT          991 a         1986 c         1895 g          958 t
ORIGIN      
        1 ggcggaagcg gccgggtctg gaccgaattg ttaacgcccg caggcgcgca ggtggtcacg
       61 acgtacgtcg acggcccgct gcccggttca cccgcgatca cccggcgcga cgtcggagcg
      121 ggcgtcgccc actacgtcac gaccgttctt gacgcgccgt ccaccagacg attgctgcac
      181 gggatctgca acgacgcagg agtggccacg cggagcaaca acaacgaggt gggtgctcct
      241 atcatcgagg cggccgggat cgagacgatc cgccggcgag ccgagggcgc aagctacctg
      301 ttcgtcatca atcacagcga tcgggaggtc gatgtgcccg cctccggagt cgaattgctg
      361 agtgggacag ctgcgaccgg atccgtgcac gtccccccgg gtggcgtagc agtcgtgcgc
      421 gaaaccacta ccgccggcgc agcgggctga tcgcggtgct cgccaagcaa cggcagtcga
      481 tgatcctcga gcacgtcagg cgctcgggcg gggtgcgcgt cagcgagctg accgaactcc
      541 tcggcgtttc ggacatgacc gttcgacgcg acctcgacgt cttggcccgg cacaacctga
      601 tcgagaaggt gcacggcggc gcgaccgtcg cgtcggcccc gagcatggac gagcccgggt
      661 tcgtcgccaa gtcgtcgcgc gagctttcgg agaaggaagc catcgcggtg gcggccgctc
      721 gactggtgcg ccccggcacc gcgattgcgc tctccggagg caccacaacg ttcgcgctcg
      781 cgcaacgcat cacccatgtc cgcgacctca ccatcgtgac caactcgatc cgggtcatcg
      841 acgtcctgca acaagcgccg ggcgcgccga cggtcatcct caccggtggg atccgcacgc
      901 cgtccgacgc gctggtcggc cccgtcgccg atctggccat ccgatcgctg cacttcgaca
      961 tcttcttcct gggctgccac ggcatggacc cgcacgccgg gctgaccacc cccaacctcg
     1021 ccgagtcaga gaccaaccgc gcgttcatcc gcggcgcacg tcaggtggtg ctgctcgccg
     1081 accacacgaa gtgggggact gtggggctca gctcgtttgc cgacctcgac gaagccgatg
     1141 tgctgatcac cgatcccggg ttgtcagacg acgcccgcac cacggcgtcc gaacacatcg
     1201 accgcgtgat catcaccggg acggggcccg acgccgaggc aggcgggctc gcgaccgttc
     1261 gccagatcta gcgcagccgg gcaccccact gtgacgtgac ggactccccc ggctcgagcc
     1321 gccgcaagcc ctggccactc tggaaggcgt tcggagccgc agtcatcggt tcaaccgcaa
     1381 ggctgcgacg ggcgagcggt accggcagct catcgcccga gtacacctgc cagaacccga
     1441 agttggcatc cgcccacacc tcgacggtgt gtccgtccga gcaggcgaca gaaacgttgg
     1501 agtgccccgc cgcatcggcg ctgacctcgg tgaatccagt gtcaaggacg cggcccgcga
     1561 gggattgctc cccgcggaag tcgtcggcgg tgcctgcgac gggctcgtcg cccacgggta
     1621 tcaaccggtc gtcgacgaga agccggcgcg acgcatcgat gtgcagcgtg cagtcgtcaa
     1681 gggtcgcgcc ggcccccgtc gacacgtaag ggtgaaagcc gagcgcgtaa ggacacgccc
     1741 agtcaccgtt gttggtcgat gtcgtcgtca cgacgagtcc gtcatcgccc agttcgtagt
     1801 tcaccagggt gtcgagccga aacggatagc cagggctggg gaggatctgg caccggagcg
     1861 tgacgtacgc cggctcttgc ttgaccaggg tccacgacct ccacgccgca aggccgtgca
     1921 gggcgttgtg gcggctgggc tcgctgagtg gtacctggtg cggttcgccg tcgaactcgt
     1981 agcggccgtc gcgcagccga ttcggccaag gcagcaagac agagccgtga aacgcccatg
     2041 agacatcgcg ctcgggatag gactggaaga tctcccgatc gccgtctcgg tattcgcgca
     2101 ccgccgcgcc gacctcgacc acggtcgccc gctggttgcc atgaccgatg cgatactgag
     2161 cgccgctcgg caccgggcgg ctcgacgagc gccgcgcagc ggtcggccct gcactcgggt
     2221 ctggcacgcg ctcagggtac acagcggccc aggcgttgtt gccaccctgc taacccacca
     2281 gagccaaacg cgtccgaaca acctgcgcta tcgttcttgt tgacttcaac aggtttgctg
     2341 tgccagcctc gggccgacct tgactcgttg cgccgatgat gaccgaccgc gaaaggcagg
     2401 cagctggtgg ttgacgctgc tcgcggccat gctcacagga ccagcacgct gttggctgat
     2461 ggccgcgagt tgatctattt cgatgagcgg gccacccaca ccgaccgcac gcgggttgtc
     2521 gatcgccggg tcttgccgac gtcgcatcct tcctcgacac tgcggctgga tcgcatcctc
     2581 gacgagtggg tggcgatcgc cggtcatcgg cagacgcgca cctatctgcc tcccgacagc
     2641 gaatgtccgc tgtgcccttc gagtgcggcc tacgcatcgg agatcccggc aaccgactac
     2701 gacgtcgtgg tgttcgagaa tcgcttcccc tcgttctcgc tgggatcctg ggtagccgaa
     2761 gaggtcgacc gcagcggggc caacggcaat ggtctgttcg cgaccgcacc gggattcggt
     2821 cgctgcgagg tcgtgtgctt tacctccgac cacgacgccg cgttctcgtc gctcacaccc
     2881 gcccgggtgc gaacggtcat cgatgcatgg gtcgatcgca ccgaagaact ctcgcggaat
     2941 ccgaagatcg agcaggtctt ctgcttcgag aaccggggcg aggaaatcgg ggtcacactc
     3001 agccacccgc acgggcagat ctacggctac ccctatgtca cacctcgcac ccggcagatg
     3061 ctggcgagtg cacgcgccca tgccgacgcc actgggcgca acctgttcgc cgacgtcgtc
     3121 acggccgaga cacagtccgg agagcgcgtc gtggtgcagt catcgtgctg gaccgcgttc
     3181 gttcccttct cggcgcgatg gccgctggaa gtacatctct acccgaaccg ccaggtgccc
     3241 gatctgccgt cgctcaccga cgaagaacgc ggcgacctga gccacgtcta cctcgaagtc
     3301 ctgcggcgaa tggaagccgt ctacgacgac tcactgccct acatcgccgc gtggcaccag
     3361 gcgccaatca gagtcgaccg cgacctcgcg tatcttcacc tcgaggtgtt ctcgatcagg
     3421 agagccccgg ggaagctgaa gtacctcgcg ggctcggagt cggccatggg ggccttcgtg
     3481 aatgacgtac tgcccgagga ggcggccaaa ctgctgcgct ccgtgatacc gaacatgacc
     3541 gacctcacag ctgacgccgc ggagtacggc gccgcggagt acggccacct tcggtgaaac
     3601 tgctcgtcac aggcggcgca ggctacatcg gaagcgtcgt cagtaggctg ctgcttgatg
     3661 aaggccacga cgtcaccgtt ctcgacgacc tctcgaccgg ccacgccgat gcggtcgccg
     3721 aaggcgccga tttcgtcgag accgacctga ctcacgccag cgacgttttg gcacgcgcac
     3781 gctacgacgg cgtcctgcat ttcgcggcga agtcgctggt cggtgagtcg atggtcaagc
     3841 cgtcgctgta ctggcgcaac aacgtcggcg gcagccgcga cctgctcgac gcggtcaccg
     3901 cacacggcgt cccgcgtttc gtcttttctt ctactgcagc ggtttacggc gagcccgacg
     3961 agataccaat ccgcgagacc gcgcgcaccc ggccgacgaa tgcctatggg gcgacaaagc
     4021 tcgccgtcga tctgatgatc cgcgacgaga cgatggcaca cggacttggc gctgtcagct
     4081 tgcgatactt caacgtcgcc ggagcatcac gtggcgcagg cgagcggcac gcgaccgaga
     4141 cgcacctgat tccgatcgcc ctcgacgtcg ctgacggcct gcgtcctgca ctcgatgtct
     4201 acggcaccga ctggccgact cccgacggca cgtgtattcg cgactacgtc cacgtcgaag
     4261 acctcgcccg agcacacgtg ctcgcgctgc acgcggcaca cgaaggtacg catctcgtgt
     4321 gcaatctcgg gaacggcggt gggttctcgg ttcgagcggt cgtcgacacc gtgcaggtcg
     4381 tgacgcgtcg cgatgtaccg attcgcaaga cggcacggcg ggcgggcgac cctgcacagc
     4441 tagtcgcgtc gcacgatgtc gcgtccgccc agctcgggtg gacgccgaca gccgacctga
     4501 ccagcatcat ctccgacgcg tgggatttcc accgcagcat caagaagacg gcgcagtcat
     4561 gactgcgacc gacctgtttg cgagcacctt cgatcggccg ccgaccgggg tttggtcagc
     4621 gcctggccgg gtcaacctca tgggcgagca caccgactac aacggcggct acgtcctgcc
     4681 gttcgccatc aacctgcgca cgcaggctgc ggcggcgcgt cgcggtgacg accgagtctc
     4741 ggtgcagtcg cttcagcgac caggcgagtt catcgagttc gctctcgacg ggctgagccc
     4801 gcagatcggt agcggatggg ccagctatgc catcggtgtg ttgtgggcgt tgcgcggcgc
     4861 cgggcaccac atcggcggtc tcgacatcgt gctcgacggt caggtaccga gcggcgctgg
     4921 cctgtcgtcg tccgccgcga tggaatgtgc agtcggcctc gccgccgccg agctgtatga
     4981 gatcgacatc cctggtgccg aactggcccg gatcgcgcag tacgccgaga acaacttcgt
     5041 cggggttccg tgcggcctga tggatcagat ggccgcctgc gtgtgtcaag ccgaccatgc
     5101 gctcttcttc gacgtgcgcg acgacgtcag ggagcaggag ccgttcgcgc cggatgacga
     5161 aggtctcgcc gtcctggtga tcgatacccg ggctcaccac gcgcacgctg gcggcggata
     5221 cgccgagcga cggaaggctt gcgaggaggc ggcgaggcaa cttggagtgc cctacctgcg
     5281 ggatatccca gggtcggagc ttgagtcggc gttgggccag ctcgacgacg agctcctgcg
     5341 ccggcgcgcg cgccacatca tcaccgaaaa cgaccgcgtt ctgactaccg ccaacctctt
     5401 ggcagccggc gccttgcgta atatcgggcc gatccttacg gagtctcacg cgtcaatgcg
     5461 cgacgacttc gagatcagcg tgcctgaact cgacacggcg gttgacgccg ccatcgccgc
     5521 tggcgccctc ggtgcacgca tgaccggcgg cggtttcggc ggcagcgcga ttgcgctcat
     5581 ccacgccgcg gagctcgacg aggtcagccg cgccatcgag gccgctttcg accggttgga
     5641 gttcaccgca ccgcagatct ggtcgccgcg tgcgtcgtcg ggagcaagcc gcgagtcgtg
     5701 acaggatcac cgcggctacg cctgtggccg gtccgtcggt tagggttccg ccagggatcg
     5761 tcggcttttt tcggtgatcc gaggtgcccg agtaacacat gcacacgatc acccaggggg
     5821 ataccgcgat
//
LOCUS       DATCBJ010000238         4180 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_4546, whole genome shotgun sequence.
ACCESSION   DATCBJ010000238 DATCBJ010000000
VERSION     DATCBJ010000238.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 4180)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4180)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4180
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4546"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..357)
                     /locus_tag="VII50_04865"
     CDS             complement(<1..357)
                     /locus_tag="VII50_04865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HEY5050209.1"
                     /translation="MSQRRDVLILGSTGSIGTQALDVIRRNPDLFRVVGLTSGGERLD
                     LLADQAVEFGVEVVGAAGGTAADLEAALAAASARAGVAVPTPKLLIGADAAANVASWP
                     LADGSPTGGTVLNGMTG"
     gene            complement(354..2606)
                     /locus_tag="VII50_04870"
     CDS             complement(354..2606)
                     /locus_tag="VII50_04870"
                     /inference="COORDINATES: protein motif:HMM:NF015155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HEY5050210.1"
                     /translation="MFERLGVRVFARRKRVLIVGLVLLILGGLWGGSVSKHLSSGGYY
                     DPHSESVMAGNDIQHKLGVPTQDAIVLFTSRQPVSDPTVQQAVESALAALPHAQVPSY
                     TTYWTSHQPQQLSTNGRATYVTLSLTGKTDAAQEYSLAVVRSTLAKADLPGITVQYGG
                     TVTLNKEISGQVSTDLRRAELVSLPIVLVLLIIIFGSLVSAGLPLAVGILAIVGASAV
                     LRLLTTFVSVSVFALNLVTVLGLGLAVDYALFVVSRFREELDRQPTVQRALTRTMATA
                     GRTIAFSGVTIAASLASLTLYPETFLRSMGYGGVAVVLLDVVAATTVLPALLAILGHR
                     VNALRLPVPWVKSTTKADAEHSASGQDVAVDDVAETGGWYRLAHSVMRRPLVYTVAIV
                     ALLAVMAAPFANVIFGGYTSRVLPVSAPGRVVSDTLARDFPSNATNPIQVVVEGVQNP
                     AVMPAYVKQLGSVQGAAGAAVAAVRGDNVLVQVAFNGDPISPAARATLARVRAIPPPT
                     GAHVLVGGFTAFLVDQLHSIGHVLPWMLGVMIVAMLVLLFLAFGSFVLPFKAVAMNAL
                     SIGASFGAVTWIFQSGHLSNLFGFSKLGYLDATDPILMLAVVFGLSMDYEVFLLSRIR
                     EEWDASASSGESPDARNRLAVAHGLQRSGRIITSAALLLVIVVGAFATSQVTTLKLLG
                     FGMALAIFVDATVVRAMLVPAVMRLLGATNWWAPAALARWWTSHGVGESSDAGTDPSQ
                     GTGRYETLVR"
     gene            complement(2655..3293)
                     /locus_tag="VII50_04875"
     CDS             complement(2655..3293)
                     /locus_tag="VII50_04875"
                     /inference="COORDINATES: protein motif:HMM:NF013962.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HEY5050211.1"
                     /translation="MPMLMHLRVSVPDRPGMLAQITAALADVGADIRTIAVLERESGR
                     AVDDIYLSWPEARSADVLSQRVASVRGVTVLGLRPSRQVPGAFPDIDLLSQVLATASR
                     GLDTLVDMAALAFGADWAASVTYASGSPVVLYASARPDDVIVLPEIAVIRSAAIELDG
                     VQLAATPLSPFDAVLVCGRADKPSFHQAELERIRRVTELAVGLVRADVSLAR"
     gene            3558..>4180
                     /locus_tag="VII50_04880"
     CDS             3558..>4180
                     /locus_tag="VII50_04880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009073117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEY5050212.1"
                     /translation="MTDSDRPAAVPVRGGPGLPQERRLITAIPGPRSVELLARRTEAV
                     ARGVSIALPVFIEAAGGGVVVDVDGNSLIDLGSGIAVVGVGNAADHVVSAVRNQVAAF
                     THTCFMVTPYEGYVAVCEQLNARTPGDHSKKSALFNSGAEAVENAVKIARAYTGRSAI
                     VVFEHAYHGRTNMTMALTAKNMPYKHSFGPFAPEVYRLPMAYPYRWP"
BASE COUNT          758 a         1432 c         1368 g          622 t
ORIGIN      
        1 ccccgtcatg ccgttgagca cggtcccgcc cgtcggcgaa ccgtcggcga gcggccagct
       61 cgcgacgttc gccgcggcat ccgccccgat cagcagcttc ggtgtcggca cggcgacgcc
      121 ggctcgcgcc gatgcggccg cgagcgctgc ttccaggtcg gctgccgttc cgccggcggc
      181 gccaacgacc tcgacgccaa actcgacagc ctgatcggcg agcagatcga gccgctcgcc
      241 gccactggtc agccccacca cccgaaacag gtcgggattg cgtcggatca cgtcaagggc
      301 ctgggtaccg atcgatccgg tagacccgag gatcaggacg tccctgcgct ggctcacctc
      361 accagtgtct cgtaccggcc cgtcccctgg cttggatcgg tccccgcgtc gctcgactcg
      421 cccaccccgt gactggtcca ccaccgggcc agcgccgccg gcgcccacca gttcgtcgcg
      481 cccagcaggc gcatgacggc cggcaccagc atcgcgcgga cgacggtcgc gtcaacgaag
      541 atcgccagcg ccataccgaa gccgagcaac ttgagcgtcg tgacctgcga cgtggcaaac
      601 gcgccgacca cgatgaccag caggagcgcg gcactggtga tgatgcggcc gctgcgctgg
      661 agtccgtgcg caaccgcgag ccggttgcgg gcatcgggcg attcgccgct gctcgccgag
      721 gcatcccatt cctcgcgaat gcggctgagc aggaagacct cgtagtccat tgacaaccca
      781 aacaccaccg caagcatgag gatcggatcg gtcgcgtcga ggtagccgag tttggagaac
      841 ccgaacaggt tcgaaaggtg accgctctgg aagatccacg tcaccgcccc gaacgacgcc
      901 ccgatcgaca gtgcgttcat cgcgaccgcc ttgaagggca gcacgaacga accgaacgcg
      961 aggaacagca gcacgagcat cgccacgatc atgacgccga gcatccatgg caatacgtgc
     1021 ccgatgctgt gcagctggtc aaccaggaat gccgtgaagc cgccgacgag aacgtgcgca
     1081 cccgtaggtg gcggaatcgc gcgcactcgc gcaagcgttg cccgcgcggc gggggaaata
     1141 gggtcgccgt tgaacgccac ttgcaccagc acgttgtcgc cgcggactgc ggcaaccgcg
     1201 gcgccggcgg cgccctggac cgaaccgagc tgcttcacat aggccggcat gacggccgga
     1261 ttctggacgc cctcgacgac gacctggatc ggattcgttg cgttcgaggg aaaatcacgt
     1321 gccaacgtgt cgctgaccac gcggccgggc gcgctgaccg gtagcactct cgacgtgtaa
     1381 ccaccgaaga tcacgttcgc gaacggcgct gccatcacgg cgagcaaggc gacgatcgcc
     1441 accgtgtaca cgagcggtcg ccgcatcacg ctgtgcgcca gccggtacca gccaccggtc
     1501 tctgccacgt cgtcgacggc gacgtcttgc ccggacgccg agtgctcggc gtccgccttc
     1561 gtcgtgctct tcacccacgg cactggcagg cgcagagcgt tcacccgatg accgaggatc
     1621 gccaggaggg cgggaagcac cgtcgtcgca gcaaccacgt cgagcagtac cacggcaact
     1681 ccgccgtaac ccatagagcg caggaacgtc tcggggtaca gcgtcaagga cgcgagcgag
     1741 gccgcgatgg tgacgccgga gaatgcgatc gttcgcccgg ccgtcgccat cgtgcgggtg
     1801 agggcgcgct gcacggtggg ttggcggtcg agctcctcgc ggaatcggct cacgacgaag
     1861 agggcgtagt cgactgcaag accgaggcca agcacggtca ccaggttcag cgcgaacacc
     1921 gagacgctga cgaatgtcgt cagcagccgc aggaccgcgg aggcgccgac gatggcgagg
     1981 atcccgacgg cgagcggcag cccggcggac acgaggctgc cgaaaatgat gatcagcagc
     2041 accagcacga tcggcaacga gacaagctcc gcgcgacgca agtcggtgct gacctggccg
     2101 ctgatttcct tgttcagcgt gaccgtgccg ccgtactgga cagtaatgcc gggaaggtcg
     2161 gccttggcca atgtgctgcg cacgacggcg agtgagtact cctgtgccgc atccgtcttg
     2221 ccagtcagcg acagcgtgac gtaggtcgcc cggccgttcg tcgacagctg ctgcggctgg
     2281 tggctcgtcc agtacgtcgt gtaactgggc acctgcgcat gcggcaatgc ggcaagcgcc
     2341 gattcgacgg cctgttgaac ggtcgggtcc gataccggct gccggctggt gaacagcacg
     2401 atcgcgtctt gcgtcggcac cccgagcttg tgctggatgt cgttgccggc catgactgac
     2461 tcgctgtgcg ggtcgtagta gccgccactc gagaggtgct tgctgaccga gccgccccat
     2521 aggccaccga gaatcagcag cacgagaccg acgatcagca ccctcttgcg acgggcgaac
     2581 acgcgtacac cgagacgttc gaacatagtg ttcaggctaa gcgtctcgtg cttgcggcga
     2641 aaccggcggg cctgctacct ggcgagtgac acgtccgcgc gcaccagacc caccgcaagc
     2701 tcggtcaccc ggcggatccg ctcgagctca gcctggtgga acgaaggctt gtcggcgcgt
     2761 ccgcacacga gcaccgcgtc gaacggcgac agcggcgtcg ccgccagctg aacgccgtcc
     2821 agctcgatgg cagccgagcg gataaccgcg atctccggca gcacgatcac gtcgtcgggc
     2881 cgggcacttg cgtacaggac gacggggctg cccgacgcgt acgtgacgct tgctgcccag
     2941 tcggcgccaa acgcgagggc agccatgtcg acgagggtgt cgaggccgcg cgatgcggtg
     3001 gcgagtacct ggctcagcaa gtcgatgtcg ggaaacgcac ctggcacttg gcgcgacggc
     3061 cggagcccga gcacggtcac accgcgcacg ctggcaacac gttgcgacag cacgtcggcc
     3121 gaccgagcct cgggccagct gaggtaaata tcgtcaactg ctcgaccgga ctcgcgttcg
     3181 agaaccgcga tggtccgaat gtctgcgccg acgtcggcga gcgcggcggt gatctgcgcg
     3241 agcatgccgg gacgatcggg cacgctcacc cgcaggtgca tcagcatcgg catctcccgt
     3301 cgtttcgggt agtccccgcg tcacatcatc cggccaagta caggatgtca ggtcaaggtt
     3361 tccgacaagt gaacgaattg agagagggcg gcgtcggatc tgcgacgccg ccctacccat
     3421 tcacgccaac tgggcgacgg attccgtcgc caaagtcgtc accaacggtc gttaccgtca
     3481 ggctcagcga gggcgttggt cggatacagt acatcttccc ggacatcact cttaccgccg
     3541 ggctccttgg aggcgtgatg accgactccg accgacctgc cgccgtcccc gttcgcggcg
     3601 ggccggggct gccccaggag cgccggctga tcaccgcgat cccaggcccg cgatccgtcg
     3661 agttgcttgc ccggcgaacc gaggcggtcg cgcgcggagt cagcatcgcg ctcccggtct
     3721 tcatcgaggc cgccggcggt ggcgtggtcg tcgacgtcga cggcaactcg ctgatcgacc
     3781 tcggctccgg catcgccgtg gtcggtgtgg gcaacgccgc cgaccatgtc gtgagtgcgg
     3841 tacgcaatca ggtcgctgcc ttcacgcaca cgtgtttcat ggtgacccct tatgagggat
     3901 acgtcgcggt ctgcgaacag ctcaacgcgc ggacccccgg cgaccactcg aagaagtcgg
     3961 cgctgttcaa ctcgggcgca gaagccgttg agaacgcggt caagatcgcg cgcgcgtaca
     4021 ccggccgatc cgccatcgtc gtgttcgagc acgcgtacca cggccgcacg aacatgacga
     4081 tggccctgac cgccaagaac atgccgtaca agcattcctt cggcccgttc gcgcccgagg
     4141 tctaccggct cccgatggcc tacccctacc gctggccgag
//
LOCUS       DATCBJ010000239         2038 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_93855, whole genome shotgun sequence.
ACCESSION   DATCBJ010000239 DATCBJ010000000
VERSION     DATCBJ010000239.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2038)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2038)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2038
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_93855"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1764
                     /locus_tag="VII50_04885"
     CDS             <1..1764
                     /locus_tag="VII50_04885"
                     /inference="COORDINATES: protein motif:HMM:NF015155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HEY5050213.1"
                     /translation="GVYNSLKTLRSSTLQVEFTGNAFQGVGQAKGVPPFAFGFIAALI
                     ILIFVFRTAGATALPLASAIGALGAGLGLIGILTHAMNVSNITPELTELMVLGVGVDY
                     ALFIVTRHRRNLRRGMSVHDSIATSMDTSGRAVLFAATTVCIALLGLLALGVSFFNGM
                     AIGAAVAVSLTMAASLTLLPALLSLFGLTVLPRMQRRAVRAGEFIELHATGFWARWSV
                     TVARHKALLSLGAAGVIVVLALPFFSMRLGAGDQGNDPKGSTTRSGYDLIASGFGVGY
                     NSTLEAVVDGPGATDPAYLQKVSTALQATTGVEPTSVHAIPLSSSVAFVSFKSTTSPQ
                     DAKTYALVKDLRAHTLPPIYSGTANHVYVYGDTAIQVDFAKVLAAKLPVFIAAVVGLS
                     FLLLLVAFRSLVIPLTAAVMNLLAAGASFGVVVAIFQFGWGADALGLGKGGPIDAWAP
                     VMFFAILFGLSMDYQVFLVSRMYEEWLHTHDNKRAIIVGQAETGSIITAAALIMIAVF
                     GGFVLGDQRAIKIFGIGLASAVFLDAFLLRTILVPSLMHVIGDRNWYLPKWLDRITPK
                     VSFEPPDELASEEDSVLVGNR"
     gene            1830..>2038
                     /locus_tag="VII50_04890"
     CDS             1830..>2038
                     /locus_tag="VII50_04890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5050214.1"
                     /translation="MSDILGGAEPVDDGIDEDDFDDELEDLDRAYASSGWSTTLTSPV
                     VLGIAALGLAIVSLIGLLSTYLVVNS"
BASE COUNT          322 a          645 c          646 g          425 t
ORIGIN      
        1 ggcgtctaca acagtctcaa gacactgcgg tcatcgacac tacaggtcga gttcaccgga
       61 aacgcattcc agggcgtggg tcaagcgaaa ggcgtgccgc cgttcgcctt cggtttcatc
      121 gccgcgctca tcatcttgat cttcgtcttc cgcacagctg gtgcgaccgc tttgccgttg
      181 gccagcgcta ttggcgcact cggcgcaggc cttgggctga tcggcatcct cacgcacgcg
      241 atgaacgtct cgaacatcac tcctgaactc acggagttga tggtgctggg cgtcggcgtc
      301 gactatgcgc tcttcatcgt cacccgacac cggcgaaatc tgcggcgcgg catgagcgtc
      361 cacgactcga tcgccacctc gatggacacg tcaggtcggg ctgtcctttt tgctgccacc
      421 acggtgtgca tcgcgctact cggactgctc gcgctcggcg tgagcttctt caacggtatg
      481 gcgattggcg cggcggtcgc ggtgagcctg acgatggccg cttcgctcac gttgttgccc
      541 gcactgttgt cgttgttcgg gctgacggtg ctcccccgca tgcaacgacg cgccgtgcgg
      601 gccggtgagt tcatcgagtt gcacgccacc ggcttctggg ctcgctggtc ggtgactgtt
      661 gcacgccaca aggccctgct gagcctcggc gcggccggcg tcatcgtggt cctcgcactg
      721 ccgttcttct cgatgcgact cggagctggc gaccagggca atgacccgaa aggctcgacg
      781 acccgcagcg gctacgacct gatcgcgtcg ggcttcggag tgggttacaa ctccaccctc
      841 gaagcagtgg tcgacggccc aggcgctacg gatcccgcgt acttgcagaa ggtgtccacc
      901 gcactgcagg caacgaccgg cgttgaaccg acgagtgtgc acgcgatccc gctgagcagt
      961 tcagtcgcgt tcgtctcctt caagtcgacg acctctccgc aagacgcgaa gacctacgcg
     1021 ttggtgaagg acctgcgcgc gcacacgctg ccgccgatct atagcggcac ggcgaaccac
     1081 gtttacgtct acggcgacac ggcgattcag gtcgacttcg caaaggtgct cgcggcaaaa
     1141 ctcccggtgt tcatcgccgc cgtcgtgggc ttgtcgttcc tgttgctgtt ggtcgccttc
     1201 cgcagcctgg tgatcccgtt gacggccgcc gtgatgaact tgctcgccgc tggtgcgtcg
     1261 ttcggtgtgg tcgtggccat cttccagttc gggtggggcg ccgatgccct tggccttggc
     1321 aagggtgggc cgatcgatgc gtgggcaccg gtgatgttct tcgcgatcct gttcgggtta
     1381 tcgatggact atcaggtgtt cctggtcagc cgtatgtatg aggaatggct gcatacccac
     1441 gacaacaagc gcgccatcat cgtcggccag gctgagaccg gcagcatcat cacggccgcc
     1501 gcattgatca tgatcgcggt cttcggtggt ttcgtgctgg gcgaccagcg cgcgatcaag
     1561 atcttcggta tcggcctcgc cagcgcggtg ttcctcgacg cgttcctgct ccggaccatc
     1621 ctcgtaccgt cgctgatgca cgtcatcggc gatcgcaact ggtacctgcc caagtggctc
     1681 gaccggatta cgccgaaggt gtcgttcgag ccgcccgatg agctggcctc ggaggaagac
     1741 agcgtgctgg tcggcaatcg ctagttctcg aggcaccgcg ttcatggcag gcgtgaggac
     1801 cgtggcggct tcgcgctagt gtcgtcgaca tgagcgacat cctcggtggc gcagagccag
     1861 ttgacgacgg aatcgacgag gacgacttcg acgacgaact cgaagacctc gatcgtgcgt
     1921 acgcatcgag cggctggtcg acgacgctga ccagcccggt ggttctgggc atcgccgcgt
     1981 tggggctggc catcgtcagc ctgatcgggc tgctgtcgac atacctcgtc gtgaactc
//
LOCUS       DATCBJ010000240        11175 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_68531, whole genome shotgun sequence.
ACCESSION   DATCBJ010000240 DATCBJ010000000
VERSION     DATCBJ010000240.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 11175)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11175)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11175
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_68531"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            204..671
                     /locus_tag="VII50_04895"
     CDS             204..671
                     /locus_tag="VII50_04895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5050215.1"
                     /translation="MTTIQVTERTTATPQQFLEALTDFGPDRSQIWTNSQPDFLVVHE
                     RGDTLADVTEGSKAAGAVWERLRYDWSNPTDIVLTTTDSNTWGRGSGHRYTLTATPDG
                     ATAVKAVVIRNGISVKGRMIGALLAVLGTTLIKKSLRTALRAFEARSAKPTAD"
     gene            complement(668..931)
                     /locus_tag="VII50_04900"
     CDS             complement(668..931)
                     /locus_tag="VII50_04900"
                     /inference="COORDINATES: protein motif:HMM:NF040451.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6510 family protein"
                     /protein_id="HEY5050216.1"
                     /translation="MGIHLDGNTMAGALAELFAVDVSIAVVECAGCGHTGVVAEAVVY
                     ADAPGMVARCSECSDVLLRLVRSENRAWLDMRGVVCLRLDIPD"
     gene            complement(915..1688)
                     /locus_tag="VII50_04905"
     CDS             complement(915..1688)
                     /locus_tag="VII50_04905"
                     /inference="COORDINATES: protein motif:HMM:NF012402.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin reductase"
                     /protein_id="HEY5050217.1"
                     /translation="MARAAVLGRLTWRVGTLVAFRDETAAARTLVFQIPDWPGHLAGQ
                     HLDVRLTAPDGYSTQRSYSIASAVDGDLVELTIERLPDGEVSLYLSQVLSIGDQIELR
                     GPIGGWFVWDPATDAPVLLIGGGSGVVPLMAMLRTRQRLASGTPFRLLYSVRDPAAVL
                     YEAELETLQSADPTLEVHYAFTRQTPAGHPRRAGRIDGAFLAEFGFAATLTPTVFVCG
                     PTGFVETVAGLLVAAGHDPHRVKTERFGPSGGSSDGHSS"
     gene            complement(1658..2281)
                     /locus_tag="VII50_04910"
     CDS             complement(1658..2281)
                     /locus_tag="VII50_04910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020662028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite oxidase-like oxidoreductase"
                     /protein_id="HEY5050218.1"
                     /translation="MRQREVAMPGIVSPGFFGRRHEVGPALPPGQYLVRDFPVLSAGP
                     TPRVTTETWEFRIIAETGETRTWDWAAFRALPADDVIVDLHCVTKWSKLGTSWRGVSI
                     DALLDGIATQAEYVLVHSYGGYTTNLPLRDLRHGQAWLAFAYDGSDLTPEHGGPARLL
                     VPHLYLWKSAKWVRGIDLRTTEQRGFWEQLGYHDYGDPWLEQRYSDD"
     gene            2327..3097
                     /locus_tag="VII50_04915"
     CDS             2327..3097
                     /locus_tag="VII50_04915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009080318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase family hydrolase"
                     /protein_id="HEY5050219.1"
                     /translation="MLGLPEHVTACLFDLDGVLTDTASVHDKAWTETFDAFLTARAAR
                     TGTPFVPFDPEKDYDEYVDGLPRADGVRSFLHSRGIELPEGKPDDSPDAETVNGLGNR
                     KNVVLQKHIKQDGVKVFEGSRRYLQAARDAGLRRIVVSSSANTAAVLEVTGLGEFMEG
                     RIDGVTIETDHLKGKPAPDTFLAGAKCAGVEPAAAAVFEDALAGVEAGRAGKFGCVVG
                     VNRLDATHGEQLKQHGADIVVTDLAELLGNDRDNGNTA"
     gene            3094..5460
                     /locus_tag="VII50_04920"
     CDS             3094..5460
                     /locus_tag="VII50_04920"
                     /inference="COORDINATES: protein motif:HMM:NF015588.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 65 protein"
                     /protein_id="HEY5050220.1"
                     /translation="MIDHPAYPAEPWQLRETRLNLDVIAQSESLFALANGHIGLRGNL
                     DEGEPHGIPGTYLNSFFELRPLPYAESAYGNPESSQSIVNVTNGKLIRLLVDDEPFDV
                     RYGTLVSHERVLDLRAGTLIRNAEWISPAGKRIRLRSTRVVSFTQRAIAAVEYEVEAV
                     DDSVRIVVQSELVANEATPAQTGDPREAAAMESPLQAVDQSITEQGMLLVHRTRASKL
                     QIAAGMTNVVEAPGRIDLETHYAPDWARLTAISALQPGERLTLRKYVGWGWSNVRSQE
                     ALRDQVAGAVTMARLSGFDQLLAEQRAYLDEFWDGADVDVEGDDEIQQAVRFGLFHVL
                     QASARGEGRSIAAKGLTGPGYDGHTFWDTEMFVLPVLTYTAPDAAAHALRWRYSTLEL
                     AKQRAQTLGVSGAAFAWRTIRGEECSGYWPAGTAAMHVTADIGNALARYTAVTGDTSV
                     EVEGGVELLAETARLWMSLGNFGRDGKWHIAGVTGPDEYTALVDDNAYTNLAAAHNLV
                     AAADATERHLDLSAQFGVTSSEIATWRSAAAAVCVPYNEQLRIHEQNAGFTTLPEWDF
                     EACRDKYPLLLHVPYFELYRRQVIKQADLELAMCMFGSVFDADDKARNVDYYEQRTVR
                     DSSLSANVQAVICAEVGHLELAYDYASEAALIDLHDIHHNTRDGLHMASLAGAWVSLV
                     IGFGGARDEGGELRFTPRLPDAIGRLCFNLRWRGLKLKVDVSHQEATYSLRDGSEGAL
                     TFYHEGSELTLPAGASVSRPITPFTPLLSRPKQPPGREPVARPGNPRP"
     gene            complement(5531..5749)
                     /locus_tag="VII50_04925"
     CDS             complement(5531..5749)
                     /locus_tag="VII50_04925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5050221.1"
                     /translation="MTENESFDRDDIISLDVPRLDRDRDGVESLHDTDAEQGDEEEVD
                     DLYVYDETEARAAGADLDGDARDEPRLD"
     gene            complement(5754..6812)
                     /locus_tag="VII50_04930"
     CDS             complement(5754..6812)
                     /locus_tag="VII50_04930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011718987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEY5050222.1"
                     /translation="MEYRQLGNSGLRVSALTLGTMGFGGTGWASAVGTTDVDGARRQI
                     AMALDAGVNLIDTADVYSSGLSEEIVAKALPVAREDVLLATKVRMPMGEGPNDAGLSR
                     HHVIRGCEASLRRLGTDYIDLYQIHEWDGQTPIEETLEALDDLVSAGKVRYIGVSNYA
                     GWQLMKALGISDRRGISRFVSQQIYYSLQARDAEYELVPVSLDQGLGILVWSPLAGGL
                     LSGKYRRGVEAPAGSRHLGDWSEPPIYDQDKLYDTVEALVEVADAHGVSAAQVSLAWL
                     LGSAGVTSAIVGARTEDQLADNLKAADLTLSGDERAKLDAVSAMPLLYPHWHQANTSG
                     DRLGAPELSLLGPHLRRG"
     gene            6863..8134
                     /locus_tag="VII50_04935"
     CDS             6863..8134
                     /locus_tag="VII50_04935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HEY5050223.1"
                     /translation="MTDRPTNWAGNVVFGPSTLHHPTSIDEVRRIVSGATQVRALGSG
                     HSFNPIADTIGDQISLTGMPPLLHIDSERSTVTVGGGVRYGELAPQLHRAGYALHNLG
                     SLPHICIAGATATGTHGSGAANGNLATAVRALDLVTASGDVVTLEIGDDDFAGAVVGL
                     GMLGIVTSVTLAIEPTFDVAQYVYDDLSLTQFIQHLDTTMNAAYSVSVFTDWRRPVMN
                     QVWVKHRTNAMTPGQFGTGPRFMDASLADGPRHPVPGVDPAACTKQDGVPGPWHERLP
                     HFRLDFTPSSGDELQSEYFVPRDAAVAAVTAISSIAHVIAPVLQICELRTIAADRLWL
                     SPAYSRDSVAIHFTWIRDIAAVSPVLEAIEDQLGPFDARPHWGKVFEMNADVVRGRYE
                     RMPDFSALLGRFDPTGKFRNAFVDTFVPAAG"
     gene            complement(8131..8928)
                     /locus_tag="VII50_04940"
     CDS             complement(8131..8928)
                     /locus_tag="VII50_04940"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HEY5050224.1"
                     /translation="MPPDQPLSVLGSTALAVAACRATESTRIDAWFHDELAQHVLAAA
                     VAAAGAGVGAEPALSRGLVRWVAVRTRFLDELVDRAVADGVRQVVIVGAGLDARAFRL
                     ELPRDLTVFEIDHAEVIAFKQRMLDEHEFMSTCRRRVVVADLVTDDWLQRLIDSGWTP
                     SQPTVWVAEGLLVYLNHQERTRLLTQLAAASLLGSVLGATLSTRTDNLAHPLWHPATT
                     ADPQRWLAECGWRATVHTMAEASAAYGRPLPPPFDTSTKGRLVRAVH"
     gene            complement(8918..11098)
                     /locus_tag="VII50_04945"
     CDS             complement(8918..11098)
                     /locus_tag="VII50_04945"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEY5050225.1"
                     /translation="MTELPVPERPYPVGDALDRILFRNMLDYAAEPIYFKDLQSRFIA
                     VSRALATVHGREPEELLGLTDFDVFTGEHASAAYADEQQVIATGIAMLGKEERETWRD
                     RGDTWVTTNKRPLRDLDGHIIGTFGISRDISRTVYAEQDAVRTADALALAHADLSRVE
                     AQLRDVLDTSADAIVLYDANLCYQYLNAAAMRLTEDPSGTSLGQTDRELGRDEAFLAV
                     WEAGLRGVLATGEGCSVEFSVGSGSQLRWFASHLAPQRESENGTPVGVVASTHEVTEL
                     KHAQSKLAHQAVHDPLTGLANRVLLVDRLTQALLRMERQPGQLALLFIDLDHFKVIND
                     TYGHETGDRLLVEVGERLTAIARRLDTVARFGGDEFVLLCDRLSTEEDVRVVADRVVR
                     AVGEPFFNDGIELNITASVGVVVTGDPYAGCENLVRDADAAMYQAKERGRDHYQFFDA
                     GLRDRAVAKNAVGWELAKALERGQFRLEYQPVISLRDRSIVGIEALIRWDHPERGTIP
                     PAEFIGLAESRGLIVPIGTWVLDEACRQLVEWAPLRDPARKPLGVAVNVSGRQLRRTD
                     FVSVVKVALERNGLAPNQLCLEITETALIEETAEARETLEAIAALGVHIALDDFGTGY
                     SSLAHLRQFPVDVLKIDRSFVDRLETNHRERQIVAAVTAMAHVLKMVVIAEGIETPGQ
                     LAQLTDLGCDHAQGYLLARPMRPEALALLLQAQESAHVASAGAA"
BASE COUNT         2054 a         3787 c         3518 g         1816 t
ORIGIN      
        1 gccaaccagg ccgccgcccg ccgcgccggc caccgaagcc cgcgcgaccg cacacgcgcg
       61 atcccccagc cgtaccccgt tgccagagcg acggctagcg ccacggtggg tccgtcccac
      121 atccacgacg tcacgagccc accttggcgc atcgaccgcg cgcgaaccaa caggccgcgt
      181 catcgccgca tacagtgaag ctcatgacga cgatccaggt gacggagcga accacagcga
      241 ctcctcagca gttcctcgaa gcgctgaccg acttcggccc ggaccgatcg cagatctgga
      301 ccaacagcca gccggacttc ctcgtcgtcc atgagcgcgg cgacaccttg gccgacgtga
      361 ccgagggatc caaggcagcg ggcgcggtgt gggaacggct gcgctatgac tggtcgaatc
      421 cgaccgacat cgtcctcacc acgacggata gcaacacctg gggtcggggc agcggccacc
      481 gctacaccct gaccgcgaca cccgacggcg ccacggctgt gaaggcagtc gttattcgca
      541 atggcatcag cgtcaaaggt cgcatgatcg gtgcgctgct cgcggtgctc gggacgacgc
      601 ttatcaagaa gagcctgcgc accgcactgc gcgcgttcga agcgcgatcc gcgaaaccca
      661 ccgccgacta gtctgggatg tcgaggcgca gacagacgac gccccgcatg tcgagccacg
      721 ccctgttttc ggatcgcacc aggcgaagca gcacatcgga acactccgag caccgcgcga
      781 ccatgcctgg cgcgtcggcg tagacaacgg cctcggcgac cacgccggtg tggccacacc
      841 ccgcacactc gacgacggcg atgctgacgt caacggcaaa caattcggcc agtgcgcccg
      901 ccatcgtgtt gccgtcaaga tgaatgccca tcactgctcc ctccactagg tccgaaccgc
      961 tcggtcttca ctcggtgcgg atcatggccc gcagcgacga gcagaccggc gacggtttcg
     1021 acgaagcccg tcgggccgca cacgaacacg gtcggtgtca acgtggcggc gaagccgaat
     1081 tccgcaagga acgccccgtc gattcggccg gctcgccgcg gatggccggc cggtgtctgg
     1141 cgagtaaatg cgtagtgcac ttcaagcgtc ggatctgccg actgcaacgt ctcgagttcc
     1201 gcttcgtaca gcacagccgc gggatcgcga accgaataga gcagccgaaa aggagtaccc
     1261 gaagccagcc gctgtcgggt gcgcagcatc gccattaacg gcaccacgcc ggacccgccg
     1321 ccgatcagga gcacgggcgc atcggtcgcg ggatcccaca cgaaccaacc accgatcggt
     1381 ccgcgcagct cgatctgatc gccgatcgac agcacctgcg acaaatacag cgacacctcg
     1441 ccgtccggca atcgttcgat ggtgagctcg accagatcgc cgtcgacggc agacgcgatc
     1501 gagtacgagc gctgcgtcga atacccgtcg ggtgcggtga gccggacatc gaggtgctgg
     1561 ccggcgaggt gtcctggcca gtctgggatt tggaaaacca acgtgcgggc ggccgcggtc
     1621 tcgtcgcgga acgcgaccag cgtgccgacc cgccaggtca gtcgtccgag taccgctgct
     1681 cgagccacgg atctccgtaa tcgtgataac ccaactgttc ccagaatccg cgttgctcgg
     1741 tcgtacgaag gtcgatgccg cgcacccact tcgccgactt ccacaagtag aggtgcggca
     1801 cgaggagtcg cgccggaccg ccgtgttcgg gcgtcagatc ggagccgtca tacgcgaacg
     1861 ccagccaggc ttgtccgtgg cgcaaatcgc gcaacggcag gttggtggtg tagccgccat
     1921 acgaatgcac cagcacgtac tcggcctggg tcgcgatgcc gtcgagcaac gcgtcgatgg
     1981 agacgccacg ccaggacgta ccgagctttg accatttcgt gacgcaatga aggtcgacga
     2041 tgacgtcgtc ggcgggtagc gcccggaatg ccgcccagtc ccaggtccgc gtctcccctg
     2101 tctcggcgat tatccgaaat tcccacgtct cggtcgtgac ccgcggtgtc ggccccgccg
     2161 acaacaccgg gaagtcgcgc accagatact ggcccggcgg caaggccggg ccgacctcat
     2221 gccggcgacc gaagaaccct ggcgacacga tccccggcat cgcgacctcc cgctggcgca
     2281 tcagaagcgc cgcttgggga atgctagtgc ctcaaaggag ggcctcatgc tgggtctgcc
     2341 cgaacacgtg acagcgtgct tgttcgatct cgacggcgtg ctgaccgaca ccgcctcggt
     2401 ccacgacaaa gcgtggaccg aaacgttcga cgcattcctc acggcccgcg ccgcccgtac
     2461 aggcacgccg ttcgtcccct tcgatcctga gaaggactac gacgagtacg tcgacggact
     2521 cccccgagcc gacggcgtcc gctcgttcct gcacagccgg ggcatcgagc tacccgaggg
     2581 caagcccgac gacagcccgg acgccgagac cgtcaacggg ttgggcaacc gcaagaacgt
     2641 cgtgttgcaa aagcacatca agcaggacgg cgtgaaggta ttcgagggtt cgcgtcggta
     2701 ccttcaagct gcgcgggacg ccggtctgcg gcgcatcgtc gtatcgtcaa gcgccaacac
     2761 agctgcggtg ctcgaggtca ctgggctcgg cgagttcatg gaagggcgca tcgacggcgt
     2821 gacgatcgag accgaccacc tgaagggcaa gccggcaccc gacacgtttc tcgctggcgc
     2881 gaaatgtgcc ggtgtcgagc cagccgcggc cgccgtgttc gaagatgcac tcgccggtgt
     2941 cgaagccggt cgggcgggca agttcggctg cgtcgtcggc gtcaatcgac tcgacgccac
     3001 gcacggtgag cagctcaagc agcacggcgc cgatatcgtc gtcaccgacc tcgccgagtt
     3061 gctcggcaac gacagggaca acgggaacac ggcatgatcg accacccggc ctaccccgca
     3121 gagccgtggc aactgcgcga gacgcggctc aacctcgacg tgatcgccca gtccgagtcg
     3181 ctgttcgcgc tggccaatgg gcacatcggg cttcgcggca accttgacga gggcgagccg
     3241 cacggcatcc ccggcaccta cctcaactcc ttcttcgagc tgcgcccgct gccctacgct
     3301 gagagcgcgt acggaaatcc ggagtcgagc caatcgatcg tcaacgtcac gaatggcaag
     3361 ctgatccgtc tactcgtcga cgatgaaccg ttcgacgtgc gctacggaac tctcgtgtcc
     3421 cacgagcgtg tgctcgatct gcgggccgga acactcattc gaaacgccga gtggatatcg
     3481 cctgccggca agaggattcg acttcgatcg acccgagtcg tgtcgttcac ccagcgcgcg
     3541 atcgctgcgg ttgaatacga ggtcgaagcc gtcgacgact cagtgcgcat cgtcgtgcag
     3601 tccgaactgg tggcgaacga agccacgccc gcccagaccg gggatccgcg cgaggccgct
     3661 gcaatggaaa gcccgctcca agccgtcgac caatcgatca ccgaacaagg catgcttctg
     3721 gtgcaccgca cccgcgcgag caaactccag attgctgcgg ggatgacgaa cgtcgtcgaa
     3781 gcaccgggca ggatcgacct tgaaacccac tacgcgcccg actgggcacg gctgacggcc
     3841 atcagtgcac tccagcccgg tgagcggctc acccttcgca agtacgtcgg ctggggctgg
     3901 tcgaatgtac gcagccagga ggcgctgcgc gaccaggtcg ccggcgcagt cacgatggcg
     3961 cggctgtccg ggttcgatca gcttcttgcc gaacaacgcg cctacctcga cgagttctgg
     4021 gacggcgccg acgtcgacgt cgagggcgac gacgagatcc agcaggcggt gcggttcggg
     4081 ctgtttcacg ttctgcaagc gagcgcgcgc ggtgaagggc gcagcatcgc ggcaaagggc
     4141 ctgaccgggc cgggctacga cggccacacg ttctgggaca ccgagatgtt cgtgctgccg
     4201 gtcctcacct acacagcccc cgatgcagcc gctcatgcac tgcggtggcg ctattcgacc
     4261 ctcgagctgg ccaaacaacg cgcccagaca ctgggcgtct cgggcgcagc tttcgcgtgg
     4321 cggaccatcc gaggcgagga atgctccggg tactggcctg ccgggactgc agcgatgcat
     4381 gtgacggcag acatcggcaa cgccctcgcg cgctacaccg cagtcaccgg ggatacctcg
     4441 gtcgaggtcg agggcggcgt cgaactgctc gcggagactg cgcgactgtg gatgtcgctg
     4501 ggcaacttcg gccgcgatgg caagtggcac atcgccggcg tcacgggacc agacgaatac
     4561 accgcgcttg tggacgacaa cgcctacacg aacctggctg cggcgcacaa cctcgtcgca
     4621 gcggccgacg ccaccgaacg ccatctcgac ctgtcggcac aattcggcgt cacctcgagc
     4681 gagatcgcaa cgtggcgatc ggccgccgct gcggtctgcg tgccctacaa cgagcagctg
     4741 cggattcatg aacagaacgc cggcttcacc acgctacccg aatgggactt tgaagcctgc
     4801 agggacaagt acccactgct cttgcatgtg ccgtacttcg agctctatcg gcggcaggtc
     4861 ataaagcagg ccgatcttga gctggcaatg tgcatgttcg gaagtgtctt cgacgcggac
     4921 gacaaggctc gcaacgtcga ctactacgag cagcggacgg tgcgcgactc gtcgttgtca
     4981 gcgaatgtgc aggcggtgat ctgcgccgag gtcggccatc tggagctcgc gtacgactac
     5041 gcgtccgagg ccgcgctcat cgacctgcac gatattcacc acaacacgcg cgacggcctg
     5101 catatggcat cgctagccgg cgcatgggtg tcgctcgtga tcggcttcgg cggcgcgcgc
     5161 gacgagggcg gcgaacttcg attcaccccg cgacttccgg acgccatcgg gcgactgtgc
     5221 ttcaacctga ggtggcgagg cctgaagctg aaggtcgacg tcagccatca agaagcgacg
     5281 tattcactac gcgacggcag cgagggcgcg ctgaccttct accacgaggg gagcgagttg
     5341 actttgcccg ccggcgcgtc cgtgtcacga ccgatcaccc cattcacccc gttgctatcg
     5401 cggccgaagc agccaccagg ccgtgagccg gtagcccgtc caggaaaccc ccgcccgtag
     5461 cggtcggcac tttgcagagt cagtcaagtt gcagagtcag tcaagttgca gagtcagccg
     5521 agttgcagag tcagtcaagg cgcggttcgt ctcgcgcatc gccgtcgagg tcggcaccgg
     5581 cggcgcgcgc ctcggtctcg tcgtagacgt agagatcgtc gacctcctcc tcgtcgccct
     5641 gctccgcatc ggtgtcgtgc agcgactcga ctccgtcgcg gtcgcgatca agtcggggca
     5701 cgtccaacga gatgatgtcg tcgcgatcga aggattcgtt ctcggtcatt tattcagccc
     5761 cgtcgaagat gtggtccgag caaactcagc tcgggcgcgc caagccgatc gcccgacgtg
     5821 ttggcttgat gccagtgcgg atacagcaag ggcatcgcgc tcacagcgtc gagcttcgcg
     5881 cgttcgtcgc cggacaatgt gagatccgca gccttgaggt tgtcggcgag ttggtcctcc
     5941 gtgcgcgctc cgacgattgc cgaggtcacg ccggcgctgc ccagcagcca ggcgagcgaa
     6001 acctgtgcgg cggaaacacc atgggcgtcg gctacctcaa cgagcgcttc gacagtgtcg
     6061 tacagcttgt cctggtcgta gatcggaggc tccgaccagt cgccgaggtg gcgactacct
     6121 gctggcgctt cgacgccacg tcggtacttg cccgacaaca ggccaccggc gagcgggctc
     6181 cagaccagaa tgccaagtcc ttggtcgagg ctgaccggga cgagttcgta ttcggcatcc
     6241 cgcgcctgca gcgagtagta gatctgctgg ctcacaaagc gcgagatgcc gcggcgatcg
     6301 gagatgccca acgccttcat cagttgccag ccggcgtagt tcgacactcc gatgtagcga
     6361 accttgccgg ccgaaaccag atcgtcgagc gcctcgagcg tctcttcgat tggtgtttgg
     6421 ccgtcccatt cgtgaatctg atacaggtcg atgtagtccg taccgaggcg ccgcaggctg
     6481 gcctcgcagc cccgaatgac gtggtgccgc gacaggcctg cgtcgttagg tccctcaccc
     6541 atcggcatcc gaaccttggt cgccagtagc acgtcctcgc gcgcaacggg cagcgccttc
     6601 gccacgatct cctccgacag gcccgacgag tagacgtcgg ccgtgtcaat gagattcacg
     6661 ccggcgtcca gcgccatcgc gatctgccgc cgcgcaccgt cgacgtccgt agtgccgacc
     6721 gccgaggccc agccggtgcc cccgaacccc atcgtcccaa gcgtgagtgc ggatacccgc
     6781 aggcctgagt tgccaagttg tctgtactcc atgcacgtat tcttgccccg cgaccaagcg
     6841 ggcgacacgg aggggacccc cgatgaccga tcgacccacg aattgggcgg ggaatgtcgt
     6901 ctttggccca tcgactctgc atcacccgac gtcgatcgac gaggtgcggc gaatcgtgag
     6961 cggcgcaacg caagtgcggg ctctgggcag cgggcactcg ttcaatccga tcgccgacac
     7021 gatcggcgac cagatctcgc tcaccggcat gccgccgttg ttgcacatcg actccgagcg
     7081 ttcgacggtg acggtggggg gcggcgtccg gtacggggag ctggcgccgc agctacatcg
     7141 ggccggctac gcgctgcaca acctcggctc gctgccacat atctgcatcg ccggcgcaac
     7201 ggcaaccggc acccacggct ccggtgcggc caatggcaac ctcgccactg cggtccgcgc
     7261 cctcgacctg gtcacggcga gcggtgatgt tgtcactctc gagataggcg acgacgattt
     7321 cgcgggcgcg gtggttgggc tcggaatgct cggcatcgtt acgtcggtga cactcgcgat
     7381 cgaaccgacc ttcgacgtcg cgcagtacgt ctacgacgac ctctccctca ctcaattcat
     7441 ccagcaccta gacacgacaa tgaacgccgc ctacagcgtg agtgtgttca ccgattggcg
     7501 gcgcccggtg atgaaccagg tgtgggtgaa gcaccgtacc aatgcgatga cgccggggca
     7561 gttcggcacc ggtccgcgat tcatggacgc gtcactcgcc gacggcccac ggcatcccgt
     7621 accgggtgtc gaccccgccg cctgcaccaa gcaggacggc gtccccgggc cgtggcacga
     7681 acggctaccg cactttcggc tcgacttcac accgagtagc ggtgatgagt tgcagtcgga
     7741 gtacttcgtc cctcgcgacg cggcggtcgc ggcggtcacc gcgatcagca gcatcgccca
     7801 cgtaatcgcg cccgtcctgc aaatctgcga gcttcgcaca atcgcggccg accggctctg
     7861 gctgagcccg gcgtacagcc gcgacagcgt cgcgatccat ttcacctgga tccgcgacat
     7921 cgccgcagtg tcgccagtgc ttgaagccat cgaggatcaa ctcggaccgt tcgacgccag
     7981 gccgcactgg ggcaaggtgt tcgagatgaa cgccgacgtc gtgcgtgggc gatacgagcg
     8041 aatgccggac ttcagcgcac tgctcggccg gttcgacccg accggcaagt tccgcaacgc
     8101 gttcgtcgat acgttcgtcc ccgcagcggg ctaatgaact gcgcggacca gcctgccttt
     8161 ggtgctcgtg tcaaacggcg gcggaagcgg ccgtccgtac gcggcgctcg cttccgccat
     8221 cgtgtgcact gtcgcccgcc agccgcattc ggccaaccac cgctgcgggt cggcggtggt
     8281 tgctgggtgc cacagcgggt gcgcgaggtt gtccgtccgg gtgctgaggg tggcaccaag
     8341 aacactgccc agaagggacg ccgcggcgag ctgcgtcagg agtcgcgtgc gctcttggtg
     8401 gttaaggtag accaacagcc cttcggccac ccacaccgtc ggctgcgagg gcgtccaccc
     8461 gctgtcaatc aggcgttgca gccagtcgtc agtgaccaga tcggcgacca ccacccggcg
     8521 cctgcaggtg ctcatgaact catgctcgtc gagcattcgc tgtttgaaag cgatcacctc
     8581 ggcatggtcg atctcgaaga ccgtgagatc acgcggaagt tcgagtcgga aggcccgcgc
     8641 gtcgagtccg gcaccgacaa tgacgacctg ccggacgccg tccgcgacag ctctgtcgac
     8701 gagttcatcg aggaatcgag tgcgaaccgc cacccaccgc acgagtcccc gcgacagcgc
     8761 cggctctgca cccaccccgg caccggccgc ggcaaccgcc gccgcgagca catgctgcgc
     8821 tagttcgtcg tggaaccacg cgtcgatccg agtgctctcg gtggcgcggc acgcggcaac
     8881 cgccagcgcg gtcgagccga gcacggacag cggctgatca ggcggcacca gccgatgcta
     8941 cgtgcgccga ctcctgggct tgaagcaaca gtgccagagc ctctggtcgc atcggccgag
     9001 ccagcaggta accctgagcg tggtcgcagc cgaggtcggt cagctgcgcg agctggccag
     9061 gcgtctcgat gccctcggcg atcacgacca tcttcaggac gtgcgccatc gcggtgaccg
     9121 cggccacgat ctgccgttcg cggtggttgg tctcgagacg gtcgacgaaa ctgcgatcga
     9181 tcttcaagac gtcgacgggg aactgccgca agtgcgcaag tgacgagtac ccggtaccga
     9241 aatcgtccaa agcgatatgg acgccgagtg ccgcgattgc ttccagggtt tcccgtgcct
     9301 cggcggtctc ttcgatcaac gcggtctcgg tgatctccag gcacaattgg ttcggcgcca
     9361 gaccgttgcg ctccaatgcc actttcacca ccgagacgaa gtcggtccta cgcagctgcc
     9421 gcccggagac gtttacggcc acgcccaacg gcttgcgcgc cggatcgcgc aatggcgccc
     9481 actcgactag ttgccggcag gcttcgtcca agacccaggt gccgatcggc acgatcagcc
     9541 cccggctctc tgcgagcccg atgaactcag cgggaggaat cgtgccgcgc tctggatgat
     9601 cccagcggat cagcgcctct atccccacga tggaccggtc gcgcagcgag atcacgggtt
     9661 ggtattcgag ccggaattgg ccgcgctcga gtgccttcgc gagctcccac ccgacggcat
     9721 tcttcgcgac cgcgcgatcg cgcaggccgg cgtcgaagaa ctggtaatgg tcgcggccgc
     9781 gttcctttgc ttggtacatc gcggcatccg cgtctcgaac gagattctca caaccggcgt
     9841 acggatcgcc ggtaacgacc accccgacgc tcgccgtgat gttcagctcg atgccgtcat
     9901 tgaagaacgg ttcgccgacg gcgcgtacca cccggtcagc aacgacgcgt acgtcctcct
     9961 cagtactcaa cctgtcgcac agcagcacga actcgtcacc tccgaatcgc gcgacggtgt
    10021 cgagccgacg tgcaatcgcc gtgagccgct ccccgacctc gaccaagaga cggtcacccg
    10081 tctcatgacc gtacgtgtcg ttgatcactt tgaagtggtc gagatcgata aagagcaggg
    10141 cgagctgccc cggctgccgt tccatgcgaa gcaaggcctg cgtcaatctg tcgacaagaa
    10201 gcactcggtt ggccaggccg gtcagcggat cgtgcacggc ctggtgagcc agcttgctct
    10261 gcgcgtgctt cagttcggtg acctcatggg tggacgcaac gacaccgacc ggcgtcccgt
    10321 tttcggactc tcgttgggga gcgagatgag aggcgaacca gcgcagttgc gaaccgcttc
    10381 cgaccgagaa ttcgactgag caaccctcgc ccgtggccag cacgcccctc aacccggcct
    10441 cccatacggc aaggaatgcc tcatcgcgac caagctcacg atcggtctgc ccgagggacg
    10501 taccactcgg gtcctcggtc agccgcatcg ccgccgcgtt caggtactgg tagcacaggt
    10561 tggcgtcgta gagcacgatc gcgtccgccg aggtgtcgag cacgtcgcgc agctgcgcct
    10621 cgacacggct cagatccgcg tgcgcgaggg caagtgcatc cgccgtcctg accgcatcct
    10681 gctccgcgta cacagtgcgg ctgatgtcgc gagagatacc gaacgtcccg atgatgtggc
    10741 cgtcgaggtc gcgcaggggg cgcttgttgg tggtgaccca cgtgtcgccc cgatcgcgcc
    10801 aggtttcccg ctcctccttg cccaacatgg cgattccagt agcaatcacc tgctgctcat
    10861 ccgcatacgc ggccgaggca tgctcgccag taaatacgtc gaagtcggtg agcccgagaa
    10921 gttcctccgg ctcacgaccg tgcaccgttg cgagggcgcg gctgaccgcg atgaatcggc
    10981 tctgcaggtc cttgaagtag atcggctcag cggcgtagtc gagcatgttg cggaacaaga
    11041 tgcggtcgag cgcatcaccg accgggtacg ggcgctcagg caccgggagc tcggtcaccg
    11101 tgccgcacct ctcgtcatgt catcccgatc agacctgtcg tccctgtcgg ccacgacgca
    11161 ggcaacgtta ggcaa
//
LOCUS       DATCBJ010000241         2337 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_49527, whole genome shotgun sequence.
ACCESSION   DATCBJ010000241 DATCBJ010000000
VERSION     DATCBJ010000241.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2337)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2337)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2337
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_49527"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..274)
                     /locus_tag="VII50_04950"
     CDS             complement(<1..274)
                     /locus_tag="VII50_04950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007034777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY5050226.1"
                     /translation="MTESLPPALIHARGLVKKFGEFVAVDGIDFDVAPGEAFGFLGPN
                     GAGKSSTMRMIGCVSPPTAGTLTILGLDPHRDGPAIRGRLGVVPQQD"
     gene            complement(303..761)
                     /locus_tag="VII50_04955"
     CDS             complement(303..761)
                     /locus_tag="VII50_04955"
                     /inference="COORDINATES: protein motif:HMM:NF025383.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4233 domain-containing protein"
                     /protein_id="HEY5050227.1"
                     /translation="MSGPPLAPSEYDGDDVALMYRDAVKGKRRLTSSVLAMEVVVFWL
                     AIIVAIVQEHVSVAAALAIGGALAVGCIVIAAMISRPWAYLAGGALQVLAIGCGFVVP
                     AMFVVGAIFAVLWVVSVRMGDAALRRAQQFEAYVGRPPTPLPAPTLKRLG"
     gene            complement(758..2089)
                     /locus_tag="VII50_04960"
     CDS             complement(758..2089)
                     /locus_tag="VII50_04960"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folylpolyglutamate synthase/dihydrofolate
                     synthase family protein"
                     /protein_id="HEY5050228.1"
                     /translation="MSEQARELERVENDLLARWPESRIEWKPGRMTALMDLLGDPHLA
                     YPSVHIAGTNGKTSTSRMIDALLRALDLRTGRFTSPHLESITERIAIDGEPISAARFI
                     ETYDEIAGFLDLADSKGDIRLSFFEAVTAMAFAAFADAPVDVAVIEVGLGGRLDATNI
                     VHAPVAVITPVDLDHMAYLGDTIAAIAGEKAGIINPDQQVIVAAQHPDAIDVIVSTAQ
                     AVGADVLREGFQFGVIRREVAVGGQVLALRGLHGVYDEVFLPLYGGYQASNASCALAA
                     VEAFAGVVEPLDADLVRAAFSTVQSPGRLEVVRTSPTILVDATHNPAGARATVAAIAE
                     SFAFRRLVGVVAVLSDKDAYGILQVLESFLAHVVVTQNNSSRSMSADELAALAVEIFG
                     ADRVDVATHLDEAISIAVERADEDDEFGGAGVLITGSVVTAGEARRLLKHR"
     gene            complement(2098..>2337)
                     /locus_tag="VII50_04965"
     CDS             complement(2098..>2337)
                     /locus_tag="VII50_04965"
                     /inference="COORDINATES: protein motif:HMM:NF021941.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5050229.1"
                     /translation="VRVAVDLSGTIDVGKERARLTKAFDTAVNEIALAEAKLADEAFI
                     AKAPPPVVDKIRARLRDAHAEVQRLRGQLDALPSK"
BASE COUNT          436 a          798 c          732 g          371 t
ORIGIN      
        1 tgtcctgttg cggaacgacg cccaaccgac ccctgatcgc cgggccgtcg cgatgcggat
       61 cgagacccag gatcgtcaac gtgccggccg tcggtggtga gacacagccg atcattctca
      121 tcgtcgatga cttgcctgcg ccgttcggac cgaggaatcc gaatgcttcc cctggtgcga
      181 catcgaagtc gatgccgtcg acagccacga actcgccgaa cttcttgacc agtccgcggg
      241 catggatcag cgctggcgga agcgactcgg tcatcccttg aagctacccc cgagtgcccg
      301 ccctacccca accgtttaag cgtcggggcg ggcagcgggg tgggtggcct cccgacatag
      361 gcctcgaact gctgcgcgcg tcgcaacgcg gcgtcgccca tgcgaacgga gacgacccac
      421 aggacggcga agatagcgcc gacaacaaac atggccggga cgacgaagcc gcagccaatc
      481 gcgagcacct ggagcgcacc acccgcgagg taggcccagg gtctgctgat cattgcggcg
      541 atcacgatgc agccgaccgc gagcgcgcca cctatcgcga gtgccgcggc gaccgacacg
      601 tgctcctgaa ctatcgcgac gatgatggcg agccagaaca cgacgacttc catcgcgagc
      661 acactcgacg tcagccgtcg cttgcccttg accgcatcgc ggtacatgag cgcaacgtcg
      721 tccccgtcat actctgacgg cgcgagcggc ggcccgctca tcggtgcttc aggagccggc
      781 gggcctcacc ggcggtcacc acggagccgg tgatcaacac gcccgcacca ccgaattcgt
      841 cgtcctcgtc agcacgttcg acggcgatgc tgatcgcttc gtcaaggtgc gtcgcgacat
      901 cgaccctatc ggcgccgaag atctcgacgg cgagtgcggc caactcgtcg gccgacatcg
      961 accgactcga attgttctgc gtgacgacga catgcgcgag gaacgactcg agcacctgga
     1021 gaatgccgta cgcgtccttg tcgctcaaca ccgccaccac gccgacgagg cgccggaagg
     1081 cgaacgactc ggcgatcgcc gcaactgtcg cgcgagcgcc agccggattg tgcgtggcgt
     1141 cgacgaggat cgtgggcgac gtacgaacca cctctaatcg acccggcgac tgcacagtcg
     1201 agaaggcagc ccgcaccaga tcggcgtcga gtggttcgac gacccccgca aatgcctcaa
     1261 cggcagcaag cgcacacgat gcattcgacg cctggtagcc gccgtacagc ggcaggaaca
     1321 cctcgtcgta gacgccgtgg agtccccgca gcgcgagcac ctgcccaccg actgcaacct
     1381 cacgcctgat cacgccgaac tggaaaccct cgcggagcac gtcggcgccc acagcctgcg
     1441 ccgtggacac gatgacatcg atggcatcgg ggtgttgcgc ggcgacgatc acctgctggt
     1501 ccgggttgat gatgccggcc ttctccccag cgatagcagc gatcgtgtcg cccagatacg
     1561 ccatgtggtc gaggtcgacc ggcgtgatca ctgcaaccgg ggcgtgcacg atattcgttg
     1621 catcgagccg gccacctagc cccacttcga tgacggcgac atcgacaggt gcatcggcga
     1681 acgccgcgaa cgccatcgcg gtgaccgctt cgaagaagga caggcggatg tcacctttgc
     1741 tgtcggccag gtcgaggaac cccgcgatct cgtcgtaggt ctcgatgaag cgcgccgcgc
     1801 tgatcggttc accgtcaatc gcgatgcgct cggtgatcga ctccaggtgc gggctggtga
     1861 accggccggt gcgcaagtca agtgcacgca gcagcgcatc gatcatgcgc gacgtcgagg
     1921 tcttgccgtt ggtgccggcg atgtgtaccg acgggtaggc caagtgcgga tcccccaaca
     1981 gatccatcag tgccgtcatg cgcccgggct tccactcgat gcgcgactcg ggccagcggg
     2041 cgagcaagtc gttctcaacg cgttccagct cgcgcgcctg ctcactcatc ggagcaatca
     2101 tttggagggc agtgcatcga gctggccacg cagccgttgc acctcggcgt gcgcatcacg
     2161 aagacgggcg cggatcttgt cgaccaccgg cggcggcgct ttcgcgatga acgcctcgtc
     2221 ggccagcttc gcttctgcga gtgcgatctc gttcacagca gtgtcgaatg ctttggtcag
     2281 ccgggcccgc tccttgccca cgtcgatcgt gccggacaga tcgacggcga ctcgcac
//
LOCUS       DATCBJ010000242         2024 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_428205, whole genome shotgun sequence.
ACCESSION   DATCBJ010000242 DATCBJ010000000
VERSION     DATCBJ010000242.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2024)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2024)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2024
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_428205"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(120..986)
                     /locus_tag="VII50_04970"
     CDS             complement(120..986)
                     /locus_tag="VII50_04970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5050230.1"
                     /translation="MTDPWTPPPLPTPPPWTTPQPWLAPQPWLTGPPPGQPQFWGPPP
                     PQPRRTGLVVGICIGVVVLLVIGFVGARAFLVAVTHGRDIAGAPGYVTFSGPTGNPFP
                     VGRPFGQACQPIRFTVEAHVPDDVYAQVVTVVNEARADGLDVTLEDRSFLWKPDSLYY
                     PPGTTSADVQRVGIFVNNGSPELLSNGQPAHVILGYDASPDADGKHDDLVGPQGTLQM
                     GTVAGDAVGQRRAVREIIALTQGVAGSSRSDSGLGSGSTLDSFSPQDIATMKKMSGCN
                     DAADSVVEHSPI"
     gene            1116..1445
                     /locus_tag="VII50_04975"
     CDS             1116..1445
                     /locus_tag="VII50_04975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5050231.1"
                     /translation="MKLNAAFEVGAAGTLTQCRYTSDPSGPTAQVEIIVGDCAKKELD
                     IDRDTLGHTFTTVSGIGDQADQEDDNIFIRKGTNWVDNNLVLLNDPAQNVQPMQTAAK
                     IVASRLP"
     gene            complement(1442..1771)
                     /locus_tag="VII50_04980"
     CDS             complement(1442..1771)
                     /locus_tag="VII50_04980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5050232.1"
                     /translation="MDIIVAQAADSKTAQADYAQELAQAQTLIATRLPAGVHVNLNTA
                     NTEGIGDNASTVTGSTAILGKPISFTGIYVISSATFFTIGYLVLGTTPPTVTAIQDQA
                     NVTLGRI"
     gene            1845..>2024
                     /locus_tag="VII50_04985"
     CDS             1845..>2024
                     /locus_tag="VII50_04985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5050233.1"
                     /translation="MAPASVDASSWVTSWHRSRLVGDPEALGDAVGVGEGDGDAGADV
                     AGGSGGSGVGVADVLV"
BASE COUNT          356 a          627 c          679 g          362 t
ORIGIN      
        1 accgagcgct atcggccgcc ccaaacgtcg acgatcggct gcatgacctg cgcgacgtgt
       61 atgcatggtt cgagaatcga ctcgacgagc tgctcgcgga acgtcgggaa gccctgtcgt
      121 cagataggtg agtgctcgac gacggagtcc gcggcgtcgt tgcaaccgga catcttcttc
      181 atcgtggcga tgtcctgggg gctgaagctg tccagcgtcg aaccgctccc cagaccggag
      241 tcgctccggc ttgacccagc aacgccttgg gtgagggcga tgatctcgcg cactgcgcgg
      301 cgctggccaa ctgcatcgcc agcgactgtc cccatctgca gagttccctg cgggccgacc
      361 aagtcgtcgt gcttgccgtc cgcgtcgggg gaagcgtcgt aaccgaggat gacgtgcgcg
      421 ggttggccat tgctgagcag ctccggcgaa ccgttgttga caaagatgcc tacccgttgg
      481 acgtcggcgc tcgtcgtgcc cggcgggtag tacagcgagt caggcttcca caggaacgag
      541 cggtcctcca aggtgacgtc gagtccatca gctcttgctt cgttgacgac ggtaaccact
      601 tgtgcgtaaa cgtcgtccgg cacgtgtgcc tcgacggtga accggatcgg ttgacacgct
      661 tgtccgaacg gccggcccac cggaaaggga tttcccgttg gtccgctgaa ggtgacgtag
      721 ccgggtgccc ctgcgatgtc gcgcccatga gtcacagcca cgagaaacgc ccgcgcgccg
      781 acgaacccga tcaccagtag cactactacg ccgatgcaaa tcccgaccac gagaccagtg
      841 cggcgtggct ggggaggtgg cggaccccag aactgcggtt gcccgggcgg cggcccagtg
      901 agccagggct gtggagcgag ccacggctgt ggagtggtcc agggcggtgg agtgggcaac
      961 ggcggtggag tccaagggtc ggtcattacc cgacacgtcg acactctctg ccgaagcctt
     1021 aatgcgccga ccgggtcgcg agggtttgcg ggctcgttac ccggacgtta ctgagatctc
     1081 gtctagtccc cagcgtgaaa agcgctgcgg ccggcatgaa gctgaacgcc gcattcgagg
     1141 ttggcgcggc aggcaccttg acgcagtgcc ggtacacaag cgatcccagc ggaccgaccg
     1201 cgcaggtcga gatcatcgtc ggcgactgcg ccaagaagga actcgacatc gatcgcgaca
     1261 ccctcggcca cacattcacg acggtcagcg gaatcggcga ccaggccgat caggaggacg
     1321 acaacatctt cattcgcaag ggcacgaatt gggtcgacaa caacttggtc ctgttgaacg
     1381 atccagcaca gaacgtgcag ccgatgcaga ccgcagctaa gatcgttgcc tcacgcctgc
     1441 cctagatccg gccgagcgtg acgttcgctt ggtcctgaat cgcggtcacc gtgggcggcg
     1501 tcgtcccgag cacgaggtag ccgatcgtga agaacgtcgc gctcgagatc acgtagatgc
     1561 cagtgaagga gatcggcttt cccaagatcg ccgtactccc ggttacggtc gaggcgttgt
     1621 ccccgatgcc ctcggtgttc gcggtattga gattgacgtg cacgccggct ggaaggcgag
     1681 ttgcgatcag ggtctgcgcc tgggcgagtt cctgcgcgta atcagcctgc gcggtcttcg
     1741 agtcggccgc ctgcgcgacg atgatgtcca tcacgttcgt cgtctgagac ccctagacgc
     1801 attccttgcc cccacccgtg ctttcctcct tcccaggccc gaacgtggct cccgcgagcg
     1861 tcgacgcttc gtcctgggtg accagctggc atcggtcgag gttggtcggc gatcctgagg
     1921 cgctcggcga cgcggtcggc gtcggtgaag gggacggcga tgcgggggcc gacgttgccg
     1981 gcggtagtgg agggagtggg gtcggcgtcg cggacgtgct tgtc
//
LOCUS       DATCBJ010000243         7181 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_530212, whole genome shotgun sequence.
ACCESSION   DATCBJ010000243 DATCBJ010000000
VERSION     DATCBJ010000243.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 7181)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7181)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7181
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_530212"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(59..820)
                     /locus_tag="VII50_04990"
     CDS             complement(59..820)
                     /locus_tag="VII50_04990"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009074248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY5050234.1"
                     /translation="MTTDFDGLVAVVTGGASGIGLQTARLLARRGAKVACLDVVIDGL
                     DADLFGVRADVTDDDSVRAAIEAVVFRFGGIDIVINNAGIGSRGDVTANDDAEWHHVL
                     DVNVVGMVRVSRAALPYLRGSSHPAIVNTGSVAAWAGLPARALYSASKGAVQALTLAM
                     AADHVHEGIRVNCVNPGTADTPWVGRLLDAADDPVAERAALEARQPLGRLVSADEIAQ
                     AIVYLASPAAASTTGTALAVDGGMYGLRLPARPPT"
     gene            complement(855..1721)
                     /locus_tag="VII50_04995"
     CDS             complement(855..1721)
                     /locus_tag="VII50_04995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009074245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HEY5050235.1"
                     /translation="MTLALARLGETGHERPYAIVDGVASSIESLTADIDGAFLDSGGV
                     ARVRSALADGTLPAGSPVESLGLRYGPPLARPGKVVCVGLNYRDHAEETHAAVPAEPI
                     LFMKDPSTVIGPDDEVLVPRLSVKTDWEVELAVVIGHTARYLSSDTEALASIAGYAIA
                     NDVSEREFQIERGGQWDKGKSCETFNPLGPWFVPADEVADPQALALRLWVNGQLRQNG
                     NTANMIFGVVEVVRYISQFMVLRPGDVINTGTPAGVAQGQPEPKPFLRAGDVLKLEID
                     GLGRQRQVLGQA"
     gene            complement(1718..2761)
                     /locus_tag="VII50_05000"
     CDS             complement(1718..2761)
                     /locus_tag="VII50_05000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEY5050236.1"
                     /translation="MTDGGPLGRGGLQVGRYGFGGAPIAGLFESVSDEQARECLAQAW
                     TAGQRYFDTAPHYGAGLSERRVGAFLAEKPSNEWVLSTKIGRLIETAESPEAAISAGF
                     VDESGVRRVFDFSRNGIRRSLDASLARLGVDHVDILFLHDPDEHWQQAIDEAWPALAE
                     LRDEGVVRSVGAGMNQTAMLTRFVRETDMDVVLLAGRYTLLDQRALDDLLPACVERGT
                     SVVLGGVFNSGLLANPHRNATFDYEPAASDVLDRARRIDEVCARHGVAIAAAALQFPL
                     AHPAITTVVVGARSAREVEENARFIETPIPEHLWADLLTAGLLRPGTPVPGLSHTTPP
                     YETPPHETVSADS"
     gene            complement(2758..3471)
                     /locus_tag="VII50_05005"
     CDS             complement(2758..3471)
                     /locus_tag="VII50_05005"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FCD domain-containing protein"
                     /protein_id="HEY5050237.1"
                     /translation="MAAQRERTHELVVRRIEADLFDGRISLGQRLPGERGMAEQLGVS
                     RASVREAMRVLEAMGIVRTAVGSGADAGAIIVADPSLALTAALRLHLATSHLVIADIV
                     ATRVLLESWAVRVAAASPDHGQYDRAQQLLDQMDDTTLPPEEFHRLDAEFHVEMTRQA
                     GNVVVAAVMASLRESIQGYVLAAVPALPDWPAMTRKLRREHRRVLAAVRANDGAAAAV
                     AVTKHIEGFYRAAGLGASS"
     gene            3545..4312
                     /locus_tag="VII50_05010"
     CDS             3545..4312
                     /locus_tag="VII50_05010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003794770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HEY5050238.1"
                     /translation="MRIALFATCLGDAMFPDVSRATVVVLERLGHTVVFPAAQTCCGQ
                     MHVNTGYIDEAGPLVRHHVETFERALETCDVVVAPSGSCVGSVRHQHAMVARANGTPE
                     LAERAEQVAARTYELSELLVDVLGVTDVGAYYPHRVTYHPTCHSLRMLHVGDKPVRLL
                     RAVRGLDLIELPAADSCCGFGGTFSVKNSDVSTAMLADKMSNVVSTRAEVCTAGDSSC
                     LMHIGGGLSRLRAGVRTVHLAEILAATEDVAAAEGDS"
     gene            4309..5838
                     /locus_tag="VII50_05015"
     CDS             4309..5838
                     /locus_tag="VII50_05015"
                     /inference="COORDINATES: protein motif:HMM:TIGR00273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0019516 - lactate oxidation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LutB/LldF family L-lactate oxidation iron-sulfur
                     protein"
                     /protein_id="HEY5050239.1"
                     /translation="MTSTFLGLPKSVTYAPEGVGNLRGDQTFPHAAREALKNAQLRRN
                     LGIATATIREKRAAVVAEVADWEQLRDAGAAIKADVMARLPELLEQLEAAVTARGGVV
                     HWARDAAEANRIIVDLVRATGEREVVKVKSMVTQEIGLNEALESAGIAPIETDLAELI
                     VQLGHDKPSHILVPAIHRNRSEIREIFLREMPDVPADLTDDPRRLAMAARAHLRRKFL
                     SAKVGISGANFAIAETGTLAVVESEGNGRMCLTLPDTLITVMGIEKLLPTWRDLEVFL
                     QLLPRSSTGERMNPYTSMWTAVTPGDGPQAFHLVLLDNGRTAALADDVGRAALHCIRC
                     SACLNVCPIYERTGGNAYGSVYPGPIGAILSPQLTGMNGHSDPNSSLPFASTLCGACY
                     SVCPVKIDIPSILVHLRARAVDHVRAQSTPNAELLSMKAVGWAMRSSRRFARAERVMR
                     LGRLLGRWPGKQGRITALPWPGSAWTTSRDLPVPPKETFREWWLRERGAPDPAENGPP
                     R"
     gene            5835..6503
                     /locus_tag="VII50_05020"
     CDS             5835..6503
                     /locus_tag="VII50_05020"
                     /inference="COORDINATES: protein motif:HMM:NF014628.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LUD domain-containing protein"
                     /protein_id="HEY5050240.1"
                     /translation="MSARDDVLTRIRSAIAGQPPSAEITRDYRIAAAEAPGTEPVIVR
                     FVDRLTDYKARVHRTDAAGLAQTLAAVLASDASVVLPPGAPPDWRYACGNRRVRVDGD
                     PAPLRAAELDGIAAVVTGCRVAIAETGTIVLDAGPDQGRRVLTLVPDHHVVVVHEEQV
                     VMSVPESLTRLDPSRPLTFISGPSATSDIEFKRVEGVHGPRVLDVIVVTASSSSLRSE
                     APRT"
     gene            complement(6468..>7181)
                     /locus_tag="VII50_05025"
     CDS             complement(6468..>7181)
                     /locus_tag="VII50_05025"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY5050241.1"
                     /translation="NVHEKLSAPVSIVLTLLICAGVGFWHGAMVTRLGVPPFIITLVT
                     FTMSQGLALAITTGTPINGISSLFSRISSFHVGNVPLPAVIFVGTAMVAWFVLERTYI
                     GRQVYAVGGNAEAARLAGIPVAARKTLAYVSSATLAGIVGILVIGRMNVADPSIGASG
                     FELTAIAAAVIGGVALTGGEGRILGIAAGAVLLEFITNGLLALRVSPYDQQMVQGGVL
                     GVAVLADRLRARRFGAQRR"
BASE COUNT         1205 a         2347 c         2406 g         1223 t
ORIGIN      
        1 gacgccgtgt tcgccggcac ggcagcacag gtctacggct tgcccgtgcg tctgtgacct
       61 acgttggggg gcgggcgggc aaccgtagtc cgtacatgcc gccgtcgacg gcgagcgcgg
      121 tacccgtagt cgacgccgcc gctggcgatg cgagatagac gatcgcctgg gcgatttcgt
      181 cggcactgac gaggcgcccg agcggctgtc gcgcttcgag cgccgcgcgt tcggcgaccg
      241 gatcgtctgc tgcgtcgagc aatcgtccga cccacggagt gtcggcggtg ccggggttca
      301 cgcagttgac gcggattcct tcgtgcacat ggtcggctgc catcgcgagc gtcagtgctt
      361 gtacggcgcc tttgcttgcg gagtacagcg cacgtgccgg caatccggcc cacgcggcga
      421 ccgacccggt gttgacgatg gccgggtgcg acgagcctcg caaatatggc agcgctgcgc
      481 ggctgacccg aaccatgccg acgacgttga cgtcgagcac gtgatgccat tccgcgtcat
      541 cgttggcggt gacgtcgcca cgcgagccga tccccgcgtt gttgatgacg atgtcgatgc
      601 ccccgaacct gaacacgacg gcctcgatcg ccgcccgcac tgaatcgtcg tcggtaacgt
      661 cggctcgcac cccgaacagg tcggcgtcga gcccgtcgat caccacgtcg aggcacgcga
      721 ccttggcgcc tcgacgtgcg agtagccgtg cggtttgcag gccgatcccc gacgcaccac
      781 cggtgacgac cgcgaccagc ccgtcgaagt ccgtcgtcat gccgctctcc tcgctcatgc
      841 ttgcccaaga cccctcacgc ttgcccaaga acttggcgct ggcgtccgag gccgtcgatt
      901 tccagcttga gcacgtcacc tgcgcgcagg aatggcttcg gctcgggctg gccttgcgcg
      961 acgccggccg gcgttccggt attgatgacg tcgccgggtc gcaacaccat gaactggctg
     1021 atgtagcgga cgacctcgac gacgccgaag atcatgttgg cggtgttgcc gttctgccga
     1081 agttgaccat tcacccatag gcgcagggcg agtgcttgcg ggtcggcgac ctcgtcggcc
     1141 ggtacgaacc acgggccgag cggattgaac gtttcgcaac tcttgccctt gtcccactgg
     1201 ccgccacgtt cgatttggaa ctcgcgctcg gagacatcgt tggcgatggc gtaacccgcg
     1261 atggacgcga gggcctccgt gtcgctgctc aggtagcggg cggtgtgacc gatgacgacg
     1321 gctagctcga cttcccagtc cgtcttgacc gaaagccgcg gcaccagaac ctcgtcatcg
     1381 ggcccgatga ccgtcgatgg atccttcatg aacaggatcg gttccgcagg tactgccgcg
     1441 tgagtctcct cggcgtggtc gcggtagttc aggcctacgc agacgacctt gccgggccgc
     1501 gcgagcggcg ggccgtaacg caggccaagc gactccacgg ggcttcctgc cggcagcgtg
     1561 ccatcggcga gagcggaccg aactcgagcc acgccgccgc tgtcgaggaa tgcgccgtcg
     1621 atgtctgcgg tcaggctctc gatgctggac gcgactccgt cgacgatggc gtagggacgt
     1681 tcgtggccgg tctctccgag gcgggctagg gcaagggtca tgaatcggcg ctcactgtct
     1741 cgtgtggtgg tgtctcgtat ggtggtgtcg tgtgtgatag tccgggtacg ggtgtgccag
     1801 gtcgaagcaa tccagcggtg agcaggtcgg cccacaggtg ctcaggaatc ggtgtctcga
     1861 tgaagcgggc gttttcttcg acctctcgag cactgcgcgc accgacgaca acagtagtga
     1921 tcgccggatg tgccaacggg aactgcagcg ccgcggccgc aatggcaacg ccatggcgcg
     1981 cgcacacctc gtcgatccgc cgtgctctgt ccagcacatc ggaagccgcc ggctcgtagt
     2041 cgaacgtcgc gttgcgatgg gggttggcca gcaggccgct gttgaagacc ccaccgagaa
     2101 cgaccgacgt gccccgctcg acgcacgcgg gtagcaggtc gtcgagtgct cgttggtcga
     2161 gcaacgtgta gcgaccggcc agtaacacga cgtccatgtc agtctcgcga acgaaccggg
     2221 tcagcatcgc ggtctggttc atccccgcgc cgaccgaccg cacgacacct tcgtcacgca
     2281 gctccgccag tgccggccac gcctcatcga tcgcttgttg ccagtgttcg tcagggtcgt
     2341 gcaggaacaa gatgtcaacg tggtcgacgc cgaggcgcgc caggctcgcg tcgagcgaac
     2401 ggcggatgcc gttgcggctg aaatcaaaca cccgacgaac accggattcg tcgacgaagc
     2461 cagccgagat ggcggcctcc ggcgactcgg cggtctcgat cagccgacct attttggtcg
     2521 ataaaaccca ttcgttactg ggtttctcgg ccaggaaggc gccgactcga cgctcggaga
     2581 gtccggctcc gtagtgcggg gcagtgtcga agtaacgctg gcccgcggtc catgcctggg
     2641 ccagacactc acgcgcctgc tcgtcgctga ccgactcgaa gaggccggcg atcggcgcgc
     2701 cgccgaaccc gtagcgaccg acctgcagcc caccgcgacc cagcgggccg ccgtccgtca
     2761 tgacgatgcc cctagcccgg ccgcgcggta gaaaccttcg atgtgtttcg tgacggcgac
     2821 ggccgcggcc gcaccgtcgt tcgcgcgcac ggcagccaac acgcgccgat gctcgcgacg
     2881 caacttgcga gtcatcgccg gccagtcggg tagcgcagga accgctgcca ggacgtagcc
     2941 ctggatcgat tctcgcagcg acgccatcac ggccgccacg actacgttgc ccgcctgccg
     3001 ggtcatctcc acgtggaact ccgcatcgag tcggtggaac tcctcggggg gcagcgtcgt
     3061 gtcatccatc tggtcgagaa gttgctgcgc cctgtcgtac tgcccatgat cgggtgacgc
     3121 agcggcgacg cgcaccgccc acgactcgag gagtacccgg gtggcgacga tgtcggcgat
     3181 caccaggtgg ctggtcgcca gatgtaaccg caacgccgcc gtcaatgcga ggctcgggtc
     3241 ggccacgatg atcgcgccgg cgtcggcacc tgagccaacg gcggtacgca cgatccccat
     3301 cgcctcgaga acccgcatcg cctcgcgcac cgaggcgcga ctcaccccga gttgctccgc
     3361 catgccgcgc tcgcccggca accgttgccc gagcgagatc cgtccgtcga agagatcggc
     3421 ctcgattcgt cgcacgacga gctcatgggt gcgctcgcgc tgggccgcca tgcgagaagg
     3481 gtagtgtggt ctgaccacat gcgctagcgt gacattcacg gctgaccaga ccccgaggag
     3541 cgacatgcgc atcgcgctgt ttgccacctg cctcggcgat gcgatgttcc ccgacgtgag
     3601 tcgcgcgacc gtcgtggtgc tcgagcgact cggccatacc gtcgtgttcc ctgccgcgca
     3661 gacctgttgt gggcagatgc acgtgaacac tggctacatc gacgaggcgg gcccgctggt
     3721 tcggcatcac gtcgagacgt tcgaacgcgc cctagagacg tgtgacgtcg tggtcgcacc
     3781 atcgggatcg tgtgtcggat cggtccgcca tcagcacgcg atggtggctc gtgcgaacgg
     3841 cacgccggag ttagcggagc gcgcagagca ggtggcggcg cgcacgtacg aactgtcgga
     3901 actgcttgtc gacgtgctgg gcgtcacgga cgtcggtgcg tactacccgc accgcgtcac
     3961 ctaccacccg acctgccatt cgttacggat gctgcacgtc ggcgacaagc cggtgcggct
     4021 gcttcgcgcc gtgcgcggtc tcgacctgat cgagctaccg gccgctgatt cgtgctgcgg
     4081 attcggtggc acgttctcgg tgaagaattc cgacgtctcg accgcgatgc tggccgacaa
     4141 gatgagcaac gtggtctcga cgcgtgccga agtgtgcacg gccggcgact cgtcgtgcct
     4201 gatgcatatc ggcggtggcc tctcgcggtt gcgagctggc gtccggaccg tgcatctcgc
     4261 cgagattctg gctgcgacag aggatgttgc tgcggcagag ggcgactcgt gacgagtacg
     4321 tttctcggcc tgccgaagtc ggttacctac gcgcccgagg gcgtcggcaa ccttcggggt
     4381 gaccagactt tcccgcacgc cgcccgcgag gcgctgaaga acgcgcagtt gcggcgcaac
     4441 ctcggcattg ctacggcgac gattcgcgag aagcgggcgg ccgtcgttgc ggaggtcgcc
     4501 gactgggaac agctgcggga cgccggcgcc gcgatcaagg ctgacgtcat ggctcggctg
     4561 cctgagcttc tcgagcaact cgaagcggcg gtgacggcac gcggcggggt cgtgcactgg
     4621 gctcgcgacg cagccgaagc aaaccgcatc atcgtcgatc tcgtgcgtgc gaccggcgag
     4681 cgcgaggtcg tgaaggtgaa gtcgatggtg acccaggaga tcgggctcaa cgaggcgctc
     4741 gaatcggcgg ggatcgcacc gatcgagacc gacctcgccg agctgatcgt gcaactcggg
     4801 catgacaagc cgagccacat cttggtgccc gcgattcacc gcaaccgatc cgagattcgg
     4861 gagatctttc tgcgcgagat gcccgatgtc cccgcggatc tcaccgacga cccgcgccgg
     4921 ttggcgatgg ccgctcgcgc ccacctgcgg aggaagttcc tgtccgccaa ggtcggcatc
     4981 agtggcgcga atttcgcgat cgcggagacc ggcacgttgg ccgtcgtcga atcggagggc
     5041 aatggccgca tgtgcctgac cttgcctgac acgctcatca cggtcatggg tatcgagaaa
     5101 ctgctcccga cctggcgtga cctcgaggtg ttcttgcaac tgctgccgcg ttcgtcgacc
     5161 ggcgagcgga tgaatccgta tacctcaatg tggaccgcgg tcacgcccgg cgatggcccg
     5221 caagcatttc atctggtgct gctcgacaac ggccggacgg cggcactggc cgatgacgtc
     5281 ggccgagccg ccctgcactg catccggtgc agtgcctgcc tcaacgtgtg cccgatttac
     5341 gaacggaccg gcggaaacgc ttatggctcg gtgtatccgg ggccgatcgg cgcgatcctc
     5401 agcccgcagc tgacggggat gaacggacac agcgatccga actcgagcct gccgttcgcc
     5461 tcgaccctat gcggcgcgtg ctattcggtg tgcccggtga agatcgatat tccgtcgatc
     5521 ctggttcatc tgcgcgcccg cgcggtcgac cacgtacgtg cacagtcgac tccgaatgcg
     5581 gaactgcttt cgatgaaagc tgtcgggtgg gcaatgcgct cgtcccgacg gttcgcccga
     5641 gccgagcgcg tgatgcgact tggccggttg ctcgggcggt ggcctggtaa gcagggtcga
     5701 atcaccgcgt tgccatggcc cggctcggcg tggacgacaa gccgcgacct gcccgttccg
     5761 ccgaaggaga ccttccgaga atggtggttg cgcgagcgcg gggcaccaga tccggccgaa
     5821 aatgggccgc ccagatgagc gcccgtgacg acgtgctgac ccgcatcaga tcagcgatcg
     5881 ccgggcaacc gccgtccgcc gaaattacgc gcgactaccg gatcgctgcc gccgaggcgc
     5941 caggcaccga gccggtcatc gtgcggttcg tcgaccggct caccgactac aaggcgcgcg
     6001 tgcatcgcac cgacgcggcc ggtctagcgc agacattggc ggcggtgttg gcgtcggacg
     6061 cgtcggtggt gttgccacct ggggcgcctc ccgactggcg gtacgcctgc ggtaatcgcc
     6121 gggtgcgtgt cgacggcgat ccggcaccgc tccgtgctgc tgaactcgat ggcatcgccg
     6181 cggtcgtgac cggttgccgg gtggcgatcg cggagaccgg cacgatcgtg ctcgatgcgg
     6241 ggccagatca gggacgccga gtgctcaccc tggtacctga ccatcacgtc gttgttgtgc
     6301 acgaagagca agttgtgatg agcgtgccgg agtcgctgac tcgtctcgat ccgtcgagac
     6361 cgctcacgtt catcagtgga ccgagcgcga cgagtgacat cgagttcaaa cgcgtcgaag
     6421 gcgtacacgg accgcgtgta cttgacgtga tcgtcgtcac cgcgtcgtca tcttcgctgc
     6481 gctccgaagc gcctcgcacg taggcggtcc gcgaggacgg cgaccccgag aacgccaccc
     6541 tgcaccatct gttggtcgta aggactgacc cgcagtgcga gcagcccgtt ggtgatgaac
     6601 tcgagaagga cggcgcccgc ggcgatgccg aggatccgac cttcgccgcc ggtgagagcg
     6661 accccaccga tgacggccgc ggcaatcgcg gtcagttcga agccgctcgc accaatcgat
     6721 gggtcggcga cattcatgcg gccgatgacg aggatcccga cgattccggc cagtgtcgcg
     6781 ctggagacat aggcgagagt cttgcgggct gcgaccggta taccggcgag gcgagccgcc
     6841 tcggcgttgc cgccgacggc atagacctgc cggcctatat acgttcgttc aaggacgaac
     6901 caggcgacca tcgcggtgcc gacgaagatg acggcgggta atggcacgtt gcccacgtgg
     6961 aacgaactga tgcgactgaa cagcgacgag atgccgttga tgggcgtgcc tgtagtgatc
     7021 gcgagcgcca aaccctgcga catcgtgaac gtcaccaacg tgatgatgaa cggcggcaca
     7081 cccaaccggg tcaccatcgc gccgtgccag aatccgacgc ctgcacagat gaggagggtg
     7141 aggacgatcg agacaggtgc cgacaacttc tcgtgcacgt t
//
LOCUS       DATCBJ010000244         3133 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_485707, whole genome shotgun sequence.
ACCESSION   DATCBJ010000244 DATCBJ010000000
VERSION     DATCBJ010000244.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 3133)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3133)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3133
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_485707"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            132..851
                     /locus_tag="VII50_05030"
     CDS             132..851
                     /locus_tag="VII50_05030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020664350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase"
                     /protein_id="HEY5050242.1"
                     /translation="MASESRLRLVSIYPVLLGTYGDGGNLRVLAMRAKARGFDVEAIE
                     LDAGEPVPNGGDLYVLGGGEDDAQVAAARALRADGGLARAAATGAVVFGVCAGYQLLG
                     EVFPGSDGVPEPGVELLDVTTDRLPKRAVGELLAVPCTSGLEQPLTGYENHAGATHLG
                     RDAQPLARVEVGVGNGAGDEGALQEHILGSYLHGPGLARNPELADLLLSWAVGSPLRP
                     WAQADVDALRAERLATVRGRR"
     gene            1033..1653
                     /locus_tag="VII50_05035"
                     /pseudo
     CDS             1033..1653
                     /locus_tag="VII50_05035"
                     /inference="COORDINATES: protein motif:HMM:NF042501.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid-like protein"
     gene            1737..2336
                     /locus_tag="VII50_05040"
     CDS             1737..2336
                     /locus_tag="VII50_05040"
                     /inference="COORDINATES: protein motif:HMM:NF001270.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HEY5050243.1"
                     /translation="MVDASLAGALLVAAPTLDDPNFARSVVLLLDHDDDGALGVVLNR
                     PSEIATRDAVPLPGMAGWADVLSEPAVVHVGGPVTPDAVVCLGRIRRQGFGGPGTAAH
                     ALPGWTPLAGVIGAVDLENEPVDLLDALDGLRVFAGYAGWGPGQLEHELRLGGWLVLP
                     AEPDDAFTASPATLWSRVLRRQGGELALLHTMPADPTNN"
     gene            2355..2654
                     /locus_tag="VII50_05045"
     CDS             2355..2654
                     /locus_tag="VII50_05045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3039 domain-containing protein"
                     /protein_id="HEY5050244.1"
                     /translation="MSDVEQLPQTEMPGLGTLTMPETDARPVPRLSHGDGDHERFAHY
                     APKDKIVEASVMGTPITALCGKVWVPSRDPKRFPVCPECKDIYENVLSGGDQPAQ"
     gene            2664..>3133
                     /locus_tag="VII50_05050"
     CDS             2664..>3133
                     /locus_tag="VII50_05050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5050245.1"
                     /translation="MPDVTDRAYADALDDSDPLRDFRRQFLPFPGLDADELIYLDGNS
                     LGRPPVGALEAINELVARDWAAGVASWGEPSRSGRPAWIELPAVVGDLLGAQFLGAAA
                     GQVLVCDSTTINLYKLAAAALDARPARRTMVTEAGNFPTDRYVLEGLASARGLHL"
BASE COUNT          442 a         1013 c         1112 g          566 t
ORIGIN      
        1 gactgtggcc gatccctggg cgagcggcgg gttgccgttc gaggccgggc tgcccggtcg
       61 acccgcgctt gatgcggtgg gcacgtacac cgccttccgg gagttgctgc accgcggggg
      121 aggcgcctcc gatggcgtct gagtcgcgac ttcggctcgt ctcgatctat cccgtgctgc
      181 tcggcaccta tggcgacggc ggcaacctgc gggttctcgc gatgcgtgcg aaggcgcgcg
      241 gtttcgacgt cgaagccatc gagttggacg ccggggagcc ggtgcctaat ggtggcgatc
      301 tgtacgtgct tggtggcggc gaggacgacg cccaagtcgc ggccgcgcgt gcgttgcgcg
      361 cggacggcgg cctcgcgcgc gctgcggcaa ccggcgctgt ggtgttcggc gtgtgcgccg
      421 gctaccagct gctcggcgag gtcttccctg gatcagacgg cgtacccgag cctggcgtcg
      481 aactgcttga cgtgacaacc gatcggctcc cgaagcgggc tgtcggagaa ctcctcgccg
      541 tgccctgcac gtcggggctc gagcagccgc tgaccggcta cgagaaccac gcaggagcga
      601 cccacctggg tcgcgacgca cagccgctcg cacgagtcga ggtcggcgtt ggcaacggcg
      661 ccggtgacga gggtgcgctc caggagcaca tcttgggcag ctacctgcac ggtccaggcc
      721 tcgcacgcaa tcccgaactg gccgatctgc tgctgtcgtg ggcggtcggt tcaccgttgc
      781 gcccttgggc ccaggcagat gtcgatgcgc tgcgggccga gcggctcgcc accgtgcgcg
      841 ggcgccggta aaaggacgac caaggattcg ggaagagggt cgtgtcggtc aggcgttgcc
      901 gtgcttcgta gcgtcaagaa ttgctaacga cctacgcgga cgccgacagc caacggttac
      961 cggacgttca gcttgctccc agctgcgctg ggcaggttct tcctgacggg ggcaggcgtc
     1021 agggaggttg cgatggtggc gttgtcaacc ttcgcgccgt cccgagctct cgacttgcga
     1081 gtgtccaggt ggctacccac ctggcggacg acacctttcg cgttcgtgta tgtggcgctg
     1141 ctcgcagcgg gcggtgtcgt gctcagcctg ctcgacaccg gcgaccacga agcgctcttg
     1201 aacgtctcga gtaccgatgt cgaccacttg tcgacgcatc cgatcttcgt gctggtgacc
     1261 agcgccctgt gggtcgacgg catcgtcgac tgcctcctgg cggtgctggt tctcggcatc
     1321 gtggccaccg tgttggaacg gcgcgtcggc acccgctggg tcgtggcgat cttcgcttct
     1381 ggtcatgtcg gtgcgacgtt gctgacggaa ggctcggttg cggtcggcgt ccactatggc
     1441 gtgttaccgg cgagcgccgt gtcgcgactc gacgttggtg tcagctacgg cctcgccgcg
     1501 atgctcggcg ctgctgcggg gctgctgccg cggcccgcgc gcaccatcgg tgtgctgggc
     1561 ggttgggcgt accttggctg gccgattgcc agcgggctcg acatgacgtc gtggggccac
     1621 gtgatcgcgt tgggaatcgg tgtcagctgg tggccgtggt tgaagcggca tgaggcgcgc
     1681 gagcgcgatg cggtgggcga ggtgcgcgag cgggatgtgg tcggcatgat ggagaaatgg
     1741 tcgatgcctc gctcgccggc gcgctcctgg tcgcggcgcc aacgcttgac gatcccaatt
     1801 tcgcccgcag cgtcgtcctc cttctcgacc acgacgatga cggcgctctc ggagtcgtcc
     1861 tcaaccggcc cagtgagatt gccacccgcg acgcagtacc gcttccgggg atggcgggat
     1921 gggcggatgt cctcagcgaa cccgcggtcg tccacgtcgg tggcccggtt acaccggatg
     1981 cggtcgtgtg cctcggccgg atccgacgtc aaggcttcgg cgggcccggg accgccgcgc
     2041 atgcactacc cggttggacg ccgctcgccg gcgtgatcgg agccgtcgat ctcgaaaacg
     2101 aacccgtcga tctgctcgac gcgctcgatg gtctgcgcgt ttttgccggc tacgcaggct
     2161 gggggccggg ccaactcgaa cacgagcttc ggctgggtgg ttggctggtt ctccccgccg
     2221 aacccgacga cgcgttcacc gcgagccccg caacgctgtg gtcgcgagtg ctgcgccgcc
     2281 agggcggcga gttggcgctg ctgcacacga tgccggcaga cccaacgaac aactgaacgt
     2341 ttaggcttgt ggatatgagc gacgtcgagc agctgcccca gaccgagatg ccaggcctcg
     2401 gcactttgac gatgccggaa accgacgctc ggccggtgcc gcggctctcg catggtgacg
     2461 gcgaccacga gcggttcgcc cactacgcgc cgaaagacaa gattgtcgag gcgtcggtca
     2521 tgggcacacc gattaccgct ttgtgcggga aggtgtgggt cccgtcgcgt gaccccaagc
     2581 gtttccctgt gtgtcccgag tgcaaagaca tctacgagaa cgtcctgtcc ggcggcgacc
     2641 agccggcgca gtaggccgag gtcatgcctg acgtcaccga tcgcgcgtac gccgacgccc
     2701 tcgacgacag cgatccgcta cgagattttc gccggcagtt cctgcctttt ccgggcctgg
     2761 acgccgatga gctgatctac ctcgacggca attcattggg ccgcccccca gtcggggcgc
     2821 tcgaggcgat caacgagctc gtcgcgcgcg actgggcggc cggcgtcgca tcgtggggtg
     2881 agccgtcacg gtccggccga ccagcttgga tcgagttgcc cgcggtcgtg ggggatctgc
     2941 tcggcgcaca gtttctcggc gccgcggccg ggcaggtgct cgtatgcgat tccaccacga
     3001 tcaatctgta caagctcgcg gcagcggcgc tcgacgcgcg gccggctcgc cggacgatgg
     3061 tcaccgaagc agggaacttc ccgaccgaca gatacgtgct cgaaggtctt gcgtcggctc
     3121 gtggcttgca cct
//
LOCUS       DATCBJ010000245         2119 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_457028, whole genome shotgun sequence.
ACCESSION   DATCBJ010000245 DATCBJ010000000
VERSION     DATCBJ010000245.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2119)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2119)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2119
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_457028"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..663)
                     /locus_tag="VII50_05055"
     CDS             complement(<1..663)
                     /locus_tag="VII50_05055"
                     /inference="COORDINATES: protein motif:HMM:NF038403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease prefix domain 1-containing protein"
                     /protein_id="HEY5050246.1"
                     /translation="MFTELLNDLRYRLRALFRRSDVERELDDELRDHVEREAAANRAA
                     GIAPEDAMRRARIAFGGMANVRERSRDGRGIRWLEIIARDLRLAGRSLRRDAGFTAIA
                     VLSLAIGICMSAGVVTMLQSVYSGGLSFRNIDGVVALYRPTSGSLSSRRYDFTGPAIR
                     SLIERSTQLRDAALLHSKALSLRTDSSDSWISGAEATPTLFSMLGARPEIGRLFGSED
                     GLP"
     gene            complement(677..1009)
                     /locus_tag="VII50_05060"
     CDS             complement(677..1009)
                     /locus_tag="VII50_05060"
                     /inference="COORDINATES: protein motif:HMM:TIGR03433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEY5050247.1"
                     /translation="MPPDRLDLPQGTLDLLILKALSLAPQHGWGISERIRQISREVLD
                     VPQGSLYPALHRLERRGWVKASWGTSDNNRRAKFYELSKRGRAQLAAEEDAWHRLSVA
                     VGLVLRTT"
     gene            complement(1085..1915)
                     /locus_tag="VII50_05065"
     CDS             complement(1085..1915)
                     /locus_tag="VII50_05065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5050248.1"
                     /translation="MLVLHFSSNARNSDVYGQEYAGHATPVTRPPIGRSRSRGPRIDF
                     GGEVITAPHSGKVIMPLDLEKIKYRTNHRAWSRGLRLFLQTTLIAVTVAAPLRAQSSW
                     GRAEVNAAVGMGFPSGGIGSHTSAGPGGLIGVGIYSPANGLGLRVEGMYQALLMSERN
                     VTVCQTGECNLKPYVSGANIDATLDIVGRSSAALAPRVYLIGGLGIYHTQALMPETTP
                     EGIHDGIMSASGAGWNAGVGAGRPIAGLWCYVEARYHGVPVALNQRGGVVPIVVGVRF
                     "
BASE COUNT          382 a          683 c          686 g          368 t
ORIGIN      
        1 gggcaatccg tcttcggagc cgaacagccg cccgatttcg ggacgtgctc cgagcatcga
       61 gaagagcgtg ggggtggcct cggcgcccga gatccagctg tccgacgagt cggtgcggag
      121 gctcagggcc ttggagtgga gcaatgcggc gtcgcgcagt tgcgtcgagc gttcgatcaa
      181 ggagcggatc gccgggccgg tgaaatcgta gcgccgcgaa ctcagcgacc cactggtcgg
      241 ccggtagagt gcgaccaccc cgtcgatgtt ccggaaggac aggccgcccg agtagaccga
      301 ctgcagcatc gtcactacgc ccgccgacat gcagatgccg atggcgaggg agagcaccgc
      361 gatcgctgtg aagccggcgt cgcgcctcag cgaacgcccg gcaagccgca gatcgcgggc
      421 gatgatttcc agccaccgaa tgccgcgccc gtcgcggcta cgctcgcgca cgtttgccat
      481 tcccccgaac gcgatgcggg cccgtcgcat ggcgtcttcc ggcgctatgc cggcggcgcg
      541 gttcgcggcc gcttctcgct ccacatggtc gcggagttcg tcgtcgagtt cgcgctccac
      601 gtcgctccgc cggaacagcg cgcggaggcg gtagcgcaga tcgttcagca actcggtgaa
      661 catgagtcgg ccggcgtcag gttgtacgaa ggaccaggcc gacggcgacc gacaggcggt
      721 gccacgcgtc ttcctcggcg gcgagctgcg ctcgccctcg tttgctcaat tcatagaact
      781 tcgctcggcg gttgttgtcc gacgtgcccc aacttgcctt gacccagcca cggcgctcca
      841 agcgatgcag cgccggatac agcgagccct ggggtacgtc gagcacctca cgcgagatct
      901 gccgaatgcg ctccgagatg ccccagccgt gctgcggggc gagcgagagc gctttgagga
      961 tgagcagatc gagcgtgccc tgcgggaggt cgagacggtc cggcggcatg ggttcccctg
     1021 tggcttctac aggacgatac ggctactcct gtagaagcgc aaggtcgact gaatccggga
     1081 caactcagaa ccggacgccg accacgatcg gcaccacacc gccgcgttga tttaaagcga
     1141 cgggcacgcc gtggtaccga gcttcgacat agcaccagag accggctatg ggccgcccgg
     1201 cgcccactcc ggcgttccac ccagcgccgg atgcggacat gataccatcg tgaatgcctt
     1261 cgggagtcgt ctcgggcata agcgcctgcg tgtggtagat gccaagcccg ccgatcaagt
     1321 agaccctggg agccagcgca gcactcgaac gaccaacgat gtccagtgtc gcatcaatat
     1381 ttgcaccgct gacatatggc ttgaggttgc actcgcctgt ttggcaaacc gtgacattgc
     1441 gctcactcat taggagcgct tggtacatcc cctcgacccg caaaccgagc ccgttcgcgg
     1501 gcgagtagat gccgaccccg atcaggccgc ccggtcctgc cgaggtgtgg ctgccgatgc
     1561 cgccgcttgg gaaccccatg ccgacggccg cattgacctc ggcgcggccc caggaactct
     1621 gggcccggag gggcgccgcg actgtgacgg cgatcagagt tgtctgaagg aacaggcgca
     1681 ggccccggct ccacgcacga tggttcgtac ggtatttgat cttctcgaga tccagtggca
     1741 ttatgacctt tcctgaatgg ggtgccgtga tgacctcacc gccgaaatcg atacgaggcc
     1801 ctctagatct cgacctcccg atgggcggtc gtgtcaccgg agtggcgtgt ccggcatact
     1861 cttgtccgta cacgtcagaa ttgcgggcgt tggaggaaaa atgcaataca agcacgcttc
     1921 gagaacccgt tgggccgtag cgatacttct gctcctcacg gcatcggcgg gtcgggcaca
     1981 acaggcgcct tcgacgcgtg gcgctccact cgactcgagt catgcgcttc agttgccgag
     2041 gtaccgctac cggctcatgg gagtgtacga tgagaccagc ggcgagccga ttgatagtgt
     2101 tctgagcctc acccgattt
//
LOCUS       DATCBJ010000246         2610 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_61276, whole genome shotgun sequence.
ACCESSION   DATCBJ010000246 DATCBJ010000000
VERSION     DATCBJ010000246.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2610)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2610)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2610
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_61276"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..813)
                     /locus_tag="VII50_05070"
     CDS             complement(<1..813)
                     /locus_tag="VII50_05070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019048033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HEY5050249.1"
                     /translation="MKKPQITKPQNEMPSADVDSFAALSSNGAIDELAASSLVAESSL
                     VADSFDSDSFDGDQFDREDRQALRRVAGLSTELQDVTEVEYRQLRLERVVLVGVWTDG
                     TLRDAENSLIELKLLAETAGSEVLDGIIQRRDKPDPATYIGSGKAKELRDIVLSTGAD
                     TVICDGELAPGQLRQLEEVVKVKVVDRTALILDIFAQHARSKEGKAQVELAQMTYLLP
                     RLRGWGESLSRQAGGQAAGGVGIGGRGPGETKIETDRRRIRARMAKLRGEIAD"
     gene            complement(877..1407)
                     /locus_tag="VII50_05075"
     CDS             complement(877..1407)
                     /locus_tag="VII50_05075"
                     /inference="COORDINATES: protein motif:HMM:NF024248.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HEY5050250.1"
                     /translation="MPLQSEGRRPATLQEMRALASPIRLRIMRLVLDRALTNKEIADL
                     LRVSPANTLHHVRTLVDAGFLVAEPVRRGSRGSRERPYRATRLSWRIDTGDVDPSGLV
                     KRASLQAFLAELEDIPPSTPFATTRLGLRLSAEHKDELLARLGDVLDEFEKLPREPAG
                     EAFAVFVAVYPRGAAS"
     gene            1466..>2610
                     /locus_tag="VII50_05080"
     CDS             1466..>2610
                     /locus_tag="VII50_05080"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY5050251.1"
                     /translation="MTEPARENPEGSPPRTGGLFHHRDFRLLWIGDAVSQIGSQVGML
                     ALPLVAVKTLHATAFEVGVLAACETAAFLLVGLPAGAWCDRVRRRPILIAGDIGRALV
                     LGSVPIAALMHRLTIGQLFIVALLTGVLTVFFDVAYQSYLPELVTNEELVEGNAKLEA
                     TRGVAAVTGPSVGGFLVQAFTAPYAVLVDAVSFLWSAGWVTAIRAPERKPIKPPQRHL
                     GKEIGEGLRFVLSNPILRKIAGCTGTANLFNSASYAVMIVFFVRTVHLSAGGIGLLFS
                     IGSIGGLVGALCSGYLMRKVGQARLIWVSALIFAPPDLLLPLTHHDARLIFAGASLFA
                     ASVGATVYNIAQVSFRQALCPRPLLGRMNATMRFLVWGTQPIGGLLGG"
BASE COUNT          412 a          873 c          842 g          483 t
ORIGIN      
        1 gtccgcgatc tcgccacgca gcttcgccat ccgggcgcgg atgcggcgcc ggtcggtttc
       61 gatctttgtc tcaccgggtc cgcggccacc gatgcccaca ccgccggccg cctgaccacc
      121 ggcctggcgc gacaacgatt caccccaccc gcggagtcgc ggcaagagat aggtcatctg
      181 ggctagctcg acctgcgcct tgccttcttt gctgcgcgca tgctgcgcga agatgtcgag
      241 gatcagggcg gtgcggtcga cgactttgac cttgaccact tcttcaagct ggcgcagctg
      301 acccggcgcg agctcgccgt cacagatcac cgtgtcggcg ccggtcgaga gcacgatgtc
      361 gcgcagctcc ttcgccttgc cggaaccgat gtaggtagcc gggtcgggct tatcgcggcg
      421 ctggatgatc ccgtcgagaa cttcagagcc ggcggtctca gcgagcagct tcagttcgat
      481 gagcgagttc tcggcatcgc gcagcgtgcc gtcggtccag acaccgacca gcaccacacg
      541 ctcgaggcgc aactgccggt actcgacctc ggtgacatcc tgcagttcgg tcgacagacc
      601 ggcgacccgg cgcagcgcct gccggtcttc gcggtcgaac tgatcaccat cgaaactgtc
      661 cgagtcaaag ctgtccgcga ccagcgagct ctcggccacg agtgagctcg ctgccagttc
      721 gtcgatcgcg ccgttgcttg acagcgcggc gaagctgtcg acgtccgcgc tgggcatctc
      781 gttttgaggc tttgtgatct gtggtttctt catcccttcg atggtcgcac ggccagccat
      841 cacgggcgaa acggtttcgc taccagcacg aacccgtcac gacgccgcgc cgcgcggata
      901 cacggcgacg aagacggcga acgcctctcc cgccggttca cgtggcagct tctcgaactc
      961 atcgaggacg tcgcctagtc gcgcaagcaa ctcgtccttg tgctccgcgg acaagcgcaa
     1021 gccaaggcgc gtggtggcga acggcgtgga cgggggaatg tcctcgagct cagccaaaaa
     1081 ggcttgcagc gaggcgcgtt tgaccaggcc ggacggatcg acgtcgccgg tatcgatgcg
     1141 ccacgagagg cgggtcgccc ggtacggacg ctcccgcgac ccgcgggagc cacggcgcac
     1201 cggctccgcg accaggaatc ccgcatcgac gagggtgcgc acgtgatgga gcgtgtttgc
     1261 cggcgacact cgcagcaggt cggcgatttc cttgttggtc aaggctcggt cgagcaccaa
     1321 ccgcatgatg cgcagccgga tcggcgacgc aagcgcgcgc atctcctgca gggtcgccgg
     1381 gcgcctgccc tccgattgca gcggcatgga gccagtgtag ctaactgatt gacaactccc
     1441 aatcagtgcg accaagctcg gcagcgtgac cgaacccgca cgcgagaacc ctgaggggtc
     1501 accgccccgc acaggaggcc tcttccacca ccgagacttc cggctcctgt ggatcggcga
     1561 cgcggtcagc cagattggct cgcaggtcgg catgttggcc ctgccgctgg tcgccgtgaa
     1621 gacgctgcac gccacggcgt tcgaggtcgg tgtcctcgcg gcgtgcgaga ccgctgcgtt
     1681 ccttttggtc gggctgcccg ccggcgcgtg gtgtgaccgt gttcggcggc ggccgatcct
     1741 gatcgcgggc gatatcgggc gcgcgctggt gctcgggagt gtgccgatcg ccgccctgat
     1801 gcaccgcctg accatcggcc agctgttcat cgtcgcgcta ctcaccggcg tgctgacggt
     1861 cttcttcgac gtcgcgtatc agagctacct gcccgagctc gtgaccaacg aggaactcgt
     1921 cgaggggaac gcgaagcttg aggccacccg cggcgttgcc gcggtgaccg gaccgagcgt
     1981 cggtggcttc ctcgttcaag cgttcaccgc cccttacgcg gtactcgttg atgcggtcag
     2041 cttcctgtgg tcagccggtt gggtcaccgc gatccgagcg cccgaacgaa agccgatcaa
     2101 gccgccacaa cggcatctgg gcaaggaaat cggcgagggc ctacggttcg tcttgagcaa
     2161 tccgatcctg cgtaagatcg ccggctgcac cggcaccgca aacctgttca acagcgccag
     2221 ctacgcggtc atgatcgtgt tcttcgttcg tactgtgcac ttgtcggccg gtggcatcgg
     2281 cttgttgttc tcgatcggct cgatcggtgg cttggtcggc gctctgtgca gcggttactt
     2341 gatgcgcaag gtcggccagg cccgtctgat ttgggtcagc gcgctgatct ttgccccgcc
     2401 tgacctgctg ttgccgctga cgcatcacga cgcacgactc atatttgctg gcgcctcctt
     2461 gttcgccgca tcggtcggcg cgaccgtgta caacatcgcc caggtgagct tccgacaagc
     2521 actgtgtcca cgcccgctgc ttgggcgaat gaacgccacg atgcgattcc tggtctgggg
     2581 gacccagccg atcggcggct tgctcggtgg
//
LOCUS       DATCBJ010000247         8412 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_115111, whole genome shotgun sequence.
ACCESSION   DATCBJ010000247 DATCBJ010000000
VERSION     DATCBJ010000247.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 8412)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8412)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8412
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_115111"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2547)
                     /locus_tag="VII50_05085"
     CDS             complement(<1..2547)
                     /locus_tag="VII50_05085"
                     /inference="COORDINATES: protein motif:HMM:NF012742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEY5050252.1"
                     /translation="MDTPPTIGVLSPFVSGFYFGGVIRGVANAAAEAGARLVAIQTSD
                     PGIVYSDRPDTAPHPNAAAWGHLAGCIVVIDGATPTYLDAIRRSGKPVVLISKQLDGV
                     VCPTVQPDNQEGVKEAVRHLIAHGHRSIAFVGMMHQDDIQERHAAYVETLIEAGIEPD
                     PTLVFSTADNEEECGLRAGQAMLADGLRSTAVMVATDVNAIGVMSALTAAGLELPRDQ
                     AIIGFDDVDAAAFMKPALSSIRQDFGALGRLAAQLLLDKLAGIDVANGQHKAPTLFVV
                     RESCGCTSEIGDASSRDKVRRTAASRRRSLEEQLDRTLTADTGASGRSVTIAAAASSI
                     IELLESALKADAVPPQTKLRGDLEALYQRSPRNQTIRGVMAAARDVGADLVAARADDG
                     GGGARRVADQIVRMMLMLSAIHSQDQFNETAYFQNALGTQYAVAMDLLRSHEEDPRSL
                     QWLERTSVRAGCLGLWSEAPGELVAGADPTLAVVGAFDRNSASGRRPPGLDETVAATV
                     FPPMRLLEIADEDLGDVVFVLPVKVRSSDWGLLSIVGQIDSKVASGRETLNQWAALLA
                     VALDHQANLASLEEQREKVERSFERERGLVEEIRLSEERYALAAQAASGGLWDWDLVK
                     DEVFYSARWMELVGCPEDAVGNTSNDWFGRVHEDDFPGLLAAIEEHVQGRSTGVELEH
                     RIRRGDGSYLWVLCRALVIRGGDGVPTRMVGSLTDVHERKELESQLRHAALYDPLTGL
                     PNRTLFLDRLRVTMARAGRHADQQFAVLFLDLDGFKLVNDSLGHLLGDSLLSSVAERI
                     SSDLRPSDTASRFGGDEFAILLDDLAAPHNPVAVAERLQERLARPFHVGGH"
     gene            complement(2618..3646)
                     /locus_tag="VII50_05090"
     CDS             complement(2618..3646)
                     /locus_tag="VII50_05090"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HEY5050253.1"
                     /translation="MGREWVEAARPRVLADAARSAALLLFVGAVVIALEVSVVPTPNF
                     PPSYHWVPWLVAAALAATGLLLWRMPERVPTLAWPLVPAVPVVVILLMALGSRDATAS
                     SQLAFSLPALFAAYHLKPVVAYLIAGEMVIAETILCLLIDSPKAVIEDSVGVSLILVG
                     IMLTLVTARNRFDEAISSLRYEAQHDAVTGLLSRRTFDLDIQQLDDDEPVSLILVDID
                     DFKTVNDTFGHDIGDETLRIVAKALSANSRQNDRAYRLGGDELAVLLTGTAPETALRR
                     AEVIRRAVETGPMAFVARRERGKVTVSLGVASLPPHAPRKSELLRAADAAMYRAKGAG
                     RNRVVAAA"
     gene            3723..5468
                     /locus_tag="VII50_05095"
     CDS             3723..5468
                     /locus_tag="VII50_05095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016461116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin carboxylase N-terminal domain-containing
                     protein"
                     /protein_id="HEY5050254.1"
                     /translation="MRKVLIANRGEIAVRIARACLDAGLASVAVYAEPDLDSMHVRAA
                     SEAYALGGTTPAESYLSIDKILAVAARSGANAVHPGYGFLAENAAFARAVVDAGLVWI
                     GPPAAAIDALGDKVRARHIAASVGAPLVPGTAEPVADAEEVVEFARQYGLPVAIKAAF
                     GGGGRGLKVARTLEEIPELFASAVREAITSFGHGECFVERYLHRPRHVETQCLADVHG
                     HVVVISTRDCSLQRRHQKLVEEAPAPFLTDAAISTLYASSKDILRAAGYVGAGTCEFL
                     LGLDGTISFLEVNARIQVEHPVTEEISGIDLIREQFRLADGEALGYDDPALRGHSIEF
                     RINGEDPGRAFFPAPGRLTTWRPPTGPGVRLDSGVEAGTVVPQAFDSLLAKLIVTGAS
                     RTQALARARRALGEFEIDGMATALPFHRAVVDDPAFAPLDPETPFTVHTRWIETEFVN
                     TIAPYDAAVDGSDDATARETVTVVVAGKRLEVVLPADLQRGASSPVRRRRSAASHAAS
                     AAGGDELTAPMQGTIVKVAVSNGDAVEVGDLVVVLEAMKMEQPLTAHRAGTIAELAAE
                     PGQTVAGGAVICEIR"
     gene            complement(5465..6112)
                     /locus_tag="VII50_05100"
     CDS             complement(5465..6112)
                     /locus_tag="VII50_05100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEY5050255.1"
                     /translation="MADGGVADGAANRRVIRVLMADDQHLVRGALAALLNLEPDLEVV
                     AQVGRGDEVVAAALDVRPDVAVLDVEMPGLDGLAAAAALLAQLPSCRVLIVTTFGRAG
                     YLRRAMESGALGFVVKDAPAEQLADAVRRVARGERVVDPTLAAATLAGGSSPLTARER
                     DALVAARDGATVADIAKRLFLSEGTVRNYLSSSISKTGTRNRVEALRVAEDNGWL"
     gene            complement(6128..7291)
                     /locus_tag="VII50_05105"
     CDS             complement(6128..7291)
                     /locus_tag="VII50_05105"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEY5050256.1"
                     /translation="MTVARAEAERRRWMPETVNDRRDGPKRWRRGGGFWLIYLAVPLG
                     SAWTHHQLAASIAGTVLLVAFGWAYLFLVPLGWWGARGARYSYLIVGVLMTLTVAATV
                     VIGQNGLMALIFVAAASIILLPGRVALGGVVVLAVTSSVLPQLIKPWHQHGLAWSIGV
                     SVALASLAVFGFSRLIRANAELAAMREEVAMLAAERERMRIARDLHDLLGHSLTTVTV
                     KAALARRLFDQDPARARAEIAEVEVLARESLADVRAAVAGYREVRLATELATAREVLE
                     AAGIDAQLPGVVEGMSPEVGGLFGWVVREGVTNVVRHSKATSVRITLDATGIEIIDDG
                     SGCARKAGQLATTHTGNGLAGLAERADALGGRLVAGPVDGSETGFRLRVEVPA"
     gene            complement(7328..8056)
                     /locus_tag="VII50_05110"
     CDS             complement(7328..8056)
                     /locus_tag="VII50_05110"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY5050257.1"
                     /translation="MNLTYLRYELVRSVRNKRAFIFSLIFPLVLFYVVAGANRHIKLD
                     GIPFPTYYLAGMASFGTMVAMTSVGARIAAERQAGWNRQLRLTPLSTRAYMRAKVLTG
                     YLTALITLVLLFIAGATLGVHLTPTNVLRMTELVLLGLIPFAALGILIGHLFTPDSIG
                     PFIGGGVSLLALLGGAYGPIGGTHGGLHLISQATPTYWLVQAGHTLAGGQSWSTRGWV
                     TVAIWAAVLGVLAARSFRRDTSRV"
     gene            complement(8053..>8412)
                     /locus_tag="VII50_05115"
     CDS             complement(8053..>8412)
                     /locus_tag="VII50_05115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY5050258.1"
                     /translation="VFATHYLEEADSYADRIVLMARGRIVADGPATEIKATVGLRTIK
                     ATLPNVPLATLSGLAGVQTAELRGESVVLHCNDSDAAIRALLRDFPAASDIEIAGAGL
                     EEAFLLLTGDEKSEELS"
BASE COUNT         1459 a         2863 c         2753 g         1337 t
ORIGIN      
        1 gtgcccgccg acatgaaacg ggcgggccaa ccgctcctgc agccgctcgg ccacagcgac
       61 cggattgtgc ggcgcagcga ggtcgtcgag caggattgcg aactcgtcac caccaaatcg
      121 cgacgccgtg tcggacggcc gcaggtcgga cgagatccgt tcggcgacgc tcgacagcaa
      181 tgagtcaccg agcaggtgtc cgaggctgtc gttgacgagc ttgaagccgt cgagatccag
      241 gaacagaacc gcgaactgct ggtcggcgtg ccggcccgcg cgggccatcg tcacccgcag
      301 acggtcgagg aacagcgtgc ggttgggcag cccggtcagc ggatcataga gcgcggcgtg
      361 ccggagctgg ctttcaagtt ccttgcgttc gtggacgtcg gtcagcgacc caaccatccg
      421 ggtcggcacg ccgtccccgc cacggatgac cagcgcgcgg cacagcaccc acaagtagct
      481 gccatcgccg cggcggatgc ggtgttcgag ttcgacgccg gtcgaacggc cctggacgtg
      541 ctcctcgatc gctgcgagca atcccgggaa gtcgtcttcg tgaacgcgac cgaaccagtc
      601 gttgctcgta ttgccgactg catcctcggg acatcccacg agttccatcc agcgcgcgga
      661 gtagaagacc tcgtccttga cgaggtccca gtcccagagc ccgccgctcg ccgcttgcgc
      721 cgccagcgca tagcgctcct cgctcagtcg gatttcctcg acgagaccac gctcgcgttc
      781 aaacgaccgc tcgactttct cccgctgctc ctcaagcgaa gccaggttcg cctggtggtc
      841 gagcgcaacc gccagcaacg ccgcccactg gttcaacgtc tcgcgaccgg acgcgacctt
      901 gctgtcgatc tggccgacga tgctgagcaa tccccagtcg ctcgaccgga ccttcacggg
      961 cagtacgaac acgacatcgc cgagatcctc gtcagcgatc tcgaggagcc gcatcggcgg
     1021 aaagaccgtg gccgcaacgg tctcgtcaag gccgggaggc cgccgacccg aggcgctgtt
     1081 gcggtcgaag gcgccgacca cggcaagtgt cgggtccgcg ccggcaacga gttcgccggg
     1141 cgcctccgac cacaaaccga ggcagccggc acgaaccgac gtgcgctcga gccactgcag
     1201 cgatcgggga tcctcctcgt ggctgcgcag caggtccatg gcgacggcgt attgggtacc
     1261 gagggcgttc tggaagtagg cggtctcgtt gaattgatcc tgggagtgga tcgcggacag
     1321 catcaacatc atgcgcacga tctggtcggc aacccgacgc gcacccccac ccccgtcgtc
     1381 cgcccgcgcg gccacgaggt cggctccaac gtcgcgagcc gcggccatga cgccgcggat
     1441 ggtctgattg cgagggctgc gctggtacaa cgcttcgagg tcaccgcgaa gcttcgtctg
     1501 cggggggacg gcgtcggcct tcaacgcact ttcgagcagt tcgatgatgg acgacgcggc
     1561 cgcggcgatc gtgaccgacc ggccagacgc tccggtgtca gcagtgagcg tgcggtcgag
     1621 ttgctcctcc aacgagcgac ggcgactggc ggcggtcctt cggaccttgt cccgcgagct
     1681 ggcgtcgccg atctcgctcg tgcacccaca ggattcgcgg acgacgaaca aggtcggcgc
     1741 cttgtgctgc ccgttcgcaa cgtcgatgcc ggccagcttg tcgagcagga gttgggccgc
     1801 cagccggcca agtgcgccga agtcctggcg gatgctcgac aacgccggtt tcatgaaggc
     1861 cgccgcgtcg acgtcgtcga atccgatgat ggcctggtcg cggggaagct cgagaccggc
     1921 cgcggtcaac gccgacatca cgccaatcgc gttcacgtcg gtggcgacca tcacggcggt
     1981 tgaccgcagg ccgtcggcga gcatcgcctg gccggcccgc aggccacatt cctcctcgtt
     2041 gtccgcggtc gagaagacca gcgtcgggtc aggctctatg cctgcctcga tcagggtctc
     2101 gacgtacgcg gcgtggcgtt cctgaatgtc gtcctggtgc atcatgccga cgaaggcgat
     2161 gctgcggtgg ccatgcgcga tgaggtgcct aacggcctcc ttgacgccct cctggttgtc
     2221 aggctgcacg gttgggcaca ccacgccgtc caactgcttg ctgatcagca cgaccggctt
     2281 gccggaccgg cggatcgcgt caaggtatgt cggcgtcgca ccgtcgatga caacgatgca
     2341 accggcgaga tggccccagg ccgcggcgtt cggatgcggc gccgtgtcgg gacggtctga
     2401 gtagacgatg ccggggtcgg aggtctggat agcgaccagt cgcgcgcccg cctcggcagc
     2461 cgcgttcgcg acccctcgga tcacgccgcc gaagtagaat ccactcacga acggcgacag
     2521 cacgccgatc gtcggcgggg tatccatgcc agccctatcg accgacgatc tcggtttctg
     2581 agcgcgattt gaggcgatct accggctcgt ggagcgctca ggcggccgcg acgaccctgt
     2641 tccgtccggc gccctttgcg cggtacatcg cagcgtcggc ggcccggagc agttccgact
     2701 tgcggggtgc gtgcggcggc agtgacgcca cgccgaggct gacggtcacc ttgccgcgct
     2761 ctcggcgcgc gacgaacgcc atcggaccgg tctcgaccgc ccggcgaatg acctccgcgc
     2821 gccgcagcgc cgtctctgga gcggtgccag tcagcaaaac cgcgagttcg tctcccccga
     2881 ggcggtacgc gcgatcgttt tggcgactgt tcgcagagag cgccttcgcc acgatgcgta
     2941 gcgtttcgtc gccgatgtcg tggccgaacg tgtcgttgac cgtcttgaag tcatcgatgt
     3001 cgacgagaat cagcgacacc ggctcatcgt cgtccaactg ctggatgtcg aggtcgaagg
     3061 ttcgacggct gagcagaccg gtgaccgcat cgtgctgggc ctcgtagcgc agcgagctga
     3121 tcgcctcgtc gaagcggttt cgggcggtga ccagggtcag catgatgccg acgaggatca
     3181 acgagacgcc gaccgagtct tcgatgaccg ccttcggcga gtcgatcagc aggcacaaga
     3241 tcgtctccgc gatgaccatc tcgccggcga tcaggtaggc gaccacgggc ttgaggtggt
     3301 aggccgcgaa gagcgccggc aatgagaatg cgagctgaga ggaggccgtg gcgtcgcggc
     3361 ttccgagcgc catgagcaag atgacgacca ccggaacggc aggcaccaac ggccacgcga
     3421 gggtagggac ccgctccggc atccgccaca ggagaagccc ggtggcagcc agcgcggccg
     3481 caaccagcca cggcacccag tgatagctgg gcgggaagtt cggggtcggc acgaccgaca
     3541 cctcgagcgc gatcactacg gcgccgacga agagcaggag cgcagccgaa cgggccgcgt
     3601 cggccagtac ccgcgggcga gcagcttcga cccactctct tcccacgccg ccctatcggc
     3661 ctcagtccgc acgaactgag gtccgtagac tcccggcaac ctacgagtgc agcggaggtc
     3721 tggtgcgcaa ggttctcatc gccaaccgag gcgagatcgc cgtgcgcatc gccagagcct
     3781 gcctggacgc cgggttggcg agcgtcgcgg tctatgcgga gcctgatctc gactcgatgc
     3841 atgtccgcgc ggcttcggag gcgtacgccc tgggcggtac gacgccggcg gagagctacc
     3901 tgtctatcga caagatcctt gcggtggctg cgcgttctgg ggctaatgcc gttcaccccg
     3961 gatacggctt ccttgcggaa aacgcggcgt tcgcgcgagc tgtggtcgac gccgggttgg
     4021 tctggatcgg ccccccggct gctgccattg acgcgctggg cgacaaggtt cgcgcccgcc
     4081 atatcgctgc cagcgtcggt gcgccactcg tgcctggtac agccgagccg gtcgccgacg
     4141 ccgaggaggt cgtcgaattc gcgcggcagt acgggctacc ggttgctatc aaggccgcct
     4201 tcggcggagg tggtcgaggg ctcaaggtgg ctcgcacgct ggaggagatc cctgagctgt
     4261 tcgcgtcggc tgtgcgcgag gcgattacct cgttcggcca cggcgaatgc tttgtcgagc
     4321 gctacctgca ccggccgcgg cacgtagaaa cgcagtgcct cgctgacgtt cacggtcacg
     4381 tcgtggtgat ctcgacgcgt gactgctcgt tgcaacgccg ccatcagaag ctagtcgaag
     4441 aagcgcctgc gccatttctc accgacgcgg caatctcgac gctgtatgcg tcgtcgaagg
     4501 acatcttgcg agcggccggc tacgtaggcg cgggcacgtg tgagttcctg ctcggtctcg
     4561 acggcacgat ctcgtttctc gaggtgaacg cccggatcca ggtcgagcac ccggtcaccg
     4621 aggagatctc cggtatcgac ttgatccgcg agcagttcag actggcggac ggcgaggcgc
     4681 tggggtacga cgatccggcg ctgcgcggtc actcgatcga gttccggatc aacggcgagg
     4741 atcccggtcg tgccttcttc cccgcgccgg gccggctcac gacgtggcgc ccgccgaccg
     4801 gacccggcgt ccggcttgac tccggcgtcg aggccggcac cgttgtgcca caggccttcg
     4861 actccttgct ggccaaactc atcgtgaccg gcgcctctcg gacccaggcg cttgcccgcg
     4921 cgcgccgcgc actcggcgag ttcgagatcg acggcatggc gactgcgctg ccgttccatc
     4981 gcgcggtcgt cgacgatccc gcgttcgcgc ccttggaccc cgagacgccg ttcacggtgc
     5041 acacccgctg gatcgagacc gagttcgtca acacgatcgc cccgtacgac gctgcggttg
     5101 atggctccga cgatgcgact gcgcgcgaga ccgtcacggt tgtggtcgcg ggcaaacgac
     5161 tcgaggtcgt gctaccggcg gaccttcaac gcggcgcgag ttcgccggtt cgccggcgcc
     5221 gcagcgcagc ctcacacgca gcgagtgccg ccggcggaga tgaactcacc gcaccgatgc
     5281 agggcaccat cgtgaaggtg gcagtgagca acggcgacgc cgtcgaggtg ggtgatctcg
     5341 tcgtcgtcct cgaggcgatg aagatggaac agccgctgac cgcacatcgg gctggcacga
     5401 tcgcggagct ggcagccgaa ccaggtcaga cggtggccgg tggcgccgtc atctgcgaaa
     5461 tccgctagag ccagccgttg tcctctgcga cccgcagtgc ctcgacgcgg ttgcgggtgc
     5521 cggtcttcga gatcgacgac gataggtagt tgcgcacagt tccttcgctc aggaacagcc
     5581 gcttcgcaat gtcagcgacg gtcgcgccgt cgcgcgcggc caccagtgcg tcgcgttcgc
     5641 gcgcagtcaa cgggctactt ccgcccgcca acgtcgccgc agcaagtgtc gggtcgacga
     5701 cacgttcgcc gcgcgccact cgacgcactg catccgcgag ctgctccgcc ggcgcgtcct
     5761 tcacaacgaa cccgagcgcg ccggactcca tcgcgcgccg caagtaaccg gcccgcccaa
     5821 aggtcgtcac gatcagcacc cggcaactcg ggagttgtgc cagtagtgct gccgcagcag
     5881 cgagtccgtc gagccccggc atctcgacgt ccaacacggc aacatcaggc cggacgtcga
     5941 gggcagccgc gacgacctcg tcgccgcgcc ccacctgcgc gacaacctcg aggtcgggct
     6001 cgaggttcag caacgcggcc aatgcacccc gcacgaggtg ctggtcgtcg gccatcagga
     6061 cgcggatcac gcggcgattc gccgcgccgt ccgccacccc gccgtccgcc atggtgggcg
     6121 tcactgacta cgccggcact tcgacgcgca agcggaagcc ggtctcgcta ccgtcgacgg
     6181 gcccggcaac caatcgcccg cctagcgcgt cggctcgttc ggcgagcccg gcaagaccgt
     6241 tgccggtgtg tgtcgtcgcg agctgtccgg ccttccgcgc acaaccggat ccgtcatcga
     6301 tgatctcgat gccggtcgcg tcgagggtga tccgcaccga cgtcgccttg ctgtgtcgca
     6361 cgacgttcgt cacgccttca cggaccaccc agccgaacag cccgccgacc tcaggcgaca
     6421 ttccctcgac gacacccggc aactgagcgt caataccggc cgcttcgagt acctcgcgcg
     6481 ccgtcgcgag ttcggtagcc agccgaacct cgcgatagcc ggcaaccgct gcccgcacgt
     6541 cggcgaggct ttcgcgtgcg agcacttcga cctcggcgat ctcggctcgc gcccgcgccg
     6601 gatcctggtc gaacagtcgc ctcgccaacg cagcctttac cgtgacagtg gtgagcgaat
     6661 gcccgagcag gtcgtgcagg tctcgcgcaa tgcgcatccg ctcacgttcg gcggcaagca
     6721 tcgcgacctc ttcgcgcatg gctgcaagct ccgcgttcgc tctgatgaga cgagagaaac
     6781 cgaacaccgc gagcgaggca agcgcgacgc tgacaccgat cgaccacgcc aggccgtgct
     6841 gatgccacgg cttgatcaat tgcggaagca cagatgacgt gacggcaaga acgaccacgc
     6901 cgccaagtgc cactcgtcct ggcaacaaga tgatcgaggc cgcggcgacg aagatcagcg
     6961 ccatcaggcc gttctggccg atgaccaccg tcgctgcgac agtcagcgtc atgagcacgc
     7021 caacgatcag gtagctgtag cgcgcgccgc gcgcacccca ccaacccagc ggtacgagga
     7081 acaggtacgc ccacccgaac gccaccagca agaccgtgcc ggcgatgctg gcggcgagct
     7141 ggtgatgcgt ccacgcactg ccgagcggca ccgcgagata gatcagccag aacccaccgc
     7201 cgcgtcgcca acgcttcggg ccgtcccgcc ggtcgttgac cgtctcaggc atccaacggc
     7261 gccgctcggc ctcggctctc gcgacggtca tcgggtcctg ctcgtaacag tcatcgacgt
     7321 cgcccgctca gacccgcgac gtgtcgcgac ggaaggaccg tgcagcaagc acgccgagca
     7381 cggctgccca gatagccacg gtgacccagc cgcgtgtcga ccaactctgc ccacctgcca
     7441 gtgtgtgacc cgcctgcacc aaccagtatg tcggcgtcgc ctgcgagatg agatgcagcc
     7501 cgccatgcgt accgccgatt ggaccgtacg ccccaccgag caacgccagg agtgatacgc
     7561 cacccccgat aaacgggccg atggagtccg gagtgaatag gtggccgatc aagattccca
     7621 gtgcggcaaa aggaatgagt ccgagaagca cgagttcggt catccgcagc acgttcgtcg
     7681 gcgtcaggtg aacgccgagc gtcgctcccg caatgaagag caggacgagc gtgatgagtg
     7741 ccgtcaaata gccggtgagc accttcgccc gcatgtatgc acgcgtgctc agcggcgtga
     7801 gtctcaactg acgattccag ccggcctgcc tttcggcggc gatccgcgca ccgaccgagg
     7861 tcatggcgac catggtcccg aacgacgcca tgcctgcgag gtagtacgtc gggaacggta
     7921 tgccgtcgag tttgatgtgc cggttggctc ctgccacgac atagaacagc acgagcggaa
     7981 agatgagcga gaagatgaac gcccgtttgt tgcgcacgct gcgaacaagt tcgtagcgaa
     8041 gataggtgag attcatgaca gctcctcgga cttctcgtcc ccggtcagga gaaggaatgc
     8101 ttcttcgagg ccggcgccgg caatctcgat gtcgctagcg gccgggaaat ctcgcagcaa
     8161 cgcgcggatc gcagcatcgg agtcattgca gtgcaacacg acggattcgc cgcgaagctc
     8221 cgcagtttgt acaccggcga gccccgaaag ggtcgcaaga ggcacgttgg gcagcgttgc
     8281 ttttatcgtg cgcagcccca cagttgcctt gatctccgtc gccgggccgt cagccacgat
     8341 gcggccacgg gccatcaaca cgatccggtc tgcgtaactg tcggcctctt cgagatagtg
     8401 cgtggcgaag ac
//
LOCUS       DATCBJ010000248         4943 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_225922, whole genome shotgun sequence.
ACCESSION   DATCBJ010000248 DATCBJ010000000
VERSION     DATCBJ010000248.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 4943)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4943)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4943
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_225922"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..326
                     /locus_tag="VII50_05120"
     CDS             <1..326
                     /locus_tag="VII50_05120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5050259.1"
                     /translation="LERLAAYWAEVMGGPQIYSQSCGDQSSLQQMHAGNGDMTDLGRR
                     FVACFVQAADDAGLPDDADFRAALRAYMEWAVSDVLVYSPQGAEVPPRLPMPHWSWDG
                     LQADD"
     gene            779..1261
                     /locus_tag="VII50_05125"
     CDS             779..1261
                     /locus_tag="VII50_05125"
                     /inference="COORDINATES: protein motif:HMM:NF013331.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II truncated hemoglobin"
                     /protein_id="HEY5050260.1"
                     /translation="MTGSGTSPSLYEWAGGEPAIARMINAFYDRVENDELLSPFFPGG
                     VASKHRQNVTTWWCEVFGGPADYTKSLGGYHTMLEHHLELDITQEQRFRFALLMSLAA
                     DDAELPSDPEFRAALVGYVEWGTRLAMHNSRPGAIVVEEAPVPRWGWGVAPPYNPGGS
                     "
     gene            1269..2225
                     /locus_tag="VII50_05130"
     CDS             1269..2225
                     /locus_tag="VII50_05130"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEY5050261.1"
                     /translation="MTPTQLRAYSAVVRHGSVKAAAAEIGVTEAAISLHLGQLRKELD
                     DQLFTRTAAGLAFTPGGLRLASRAAEMLGLQAQTIREVNQAGHGRRLMRLATTSLFAE
                     HAAPGLIELFLARADDFDVELSVRPPSQFDSLLGSRAVDVAIGPRMSSAGNALVCKPF
                     LNYHVVAVVGVGHPLATSASRPALLREQTWLLGPSAAGEDGVVPVMLRQLDIPEERQR
                     IFQSHAAALEEAKRNNGIALAVSFAVAADVANGRLVVIKGSGLQAEGGWATMTLPENV
                     TVPAAAELTRFISTPRAIQAMMKGSGVHIGHFRPSVHVTLWK"
     gene            complement(2209..3510)
                     /locus_tag="VII50_05135"
     CDS             complement(2209..3510)
                     /locus_tag="VII50_05135"
                     /inference="COORDINATES: protein motif:HMM:NF017569.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEY5050262.1"
                     /translation="MSSSDNGGLLESAATARHVTVLSGVDRAAFAVAFADRLRSAGVD
                     VGLPAASTFARALAAQPPSTRSALYWLARISLTHRHSDIEAFDRVFAAVFDDAGLHLD
                     PHARRSAPARSRAGDDRFAPASGDDGVLHQDGGGLPWATLPAIVGVVDDDESPLGMPE
                     RLPSNLVGIALTPFEDLSADELELLGVWLESAFRDWPSRRTRRVQPHAHGRYVNLRAT
                     MVRSRRSGWEAVHVVRSAPRYVPRRVVMVCDVSQSMQAHATTYLHLMRAAVLSANAEV
                     FVFATGLTRLTSVLTHRSVDVAIEQATAAVVDRFGGTRIASNLDALLRSRHGGVLRGA
                     VVVIASDGWDSDPPHELAASMARLRRRAHRVIWMNPRVAASGFAPLVGAMAAALPYCD
                     DLLPAHDLRALGDAFDTVARAGRRPRPDGVRPFVPSTTSRA"
     gene            complement(3494..4159)
                     /locus_tag="VII50_05140"
     CDS             complement(3494..4159)
                     /locus_tag="VII50_05140"
                     /inference="COORDINATES: protein motif:HMM:NF017999.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEY5050263.1"
                     /translation="MKIDNQFSVNVPIDEAWHALTDLERIAPCMPGAQLTGREGDAYL
                     GTVKVKVGPVISEFAGTASFVEKDEATYHAIIDAKGRDSRGSGNASATITAQLRSDGA
                     TRTIVMVETDMKISGKLAQFGSGMIAEVSSKLLGQFAESLEQLLASAPEVPSGTKPAA
                     EQTADGAPRAATIAAPTAFRPSAAPEPVDLTHLAGGAVAKRALPVLAGIILVGVIIYV
                     IVR"
     gene            complement(4188..>4943)
                     /locus_tag="VII50_05145"
     CDS             complement(4188..>4943)
                     /locus_tag="VII50_05145"
                     /inference="COORDINATES: protein motif:HMM:NF012234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HEY5050264.1"
                     /translation="PGVGKTTAAKSLARALDVPLIRLQCYEGLTVGEALYEWNYQRQL
                     LAIRLAEASAEAITDADLFTEAFLLERPILQAVRHRGATPPVLLIDEIDRADDEFEAL
                     LFEFLGESSVTIPELGTFTAVHPPIVVLTSNRSRELHDALRRRCLYHWIDFPTPTRAA
                     EILRRGVPAASEALIQSATQFVGRVRTLDLDKPPGMAETIDWLSALSALGAAELVRAT
                     LVSTLGAIAKTPDDRDTMLGALVEISAPAATSE"
BASE COUNT          882 a         1621 c         1588 g          852 t
ORIGIN      
        1 acctcgagcg gctggccgcg tactgggcgg aagtcatggg tggtccgcag atttactccc
       61 agtcgtgcgg cgaccagtcg tcgctgcaac agatgcatgc aggcaacggc gacatgactg
      121 atctcggacg ccgatttgtt gcctgcttcg tgcaagcggc cgacgatgcc gggttgccgg
      181 acgacgccga ctttcgcgcc gcactgcgtg cctacatgga atgggcagtc agtgacgtgc
      241 tggtctactc gccgcagggt gcggaggtac cgccgagact gccgatgccg cactggtcat
      301 gggacggtct acaagccgac gattgacaga cgtgttttcc gcccgactct cgatcccgca
      361 gacaggcagg tcggcgacgt tgtgcagcca caccgaagtc gatgacccgc cggtccgctc
      421 cgcgacacct gacctgccgg cctgcgccaa cgccgacagc tgcgccgcag acacccgtcg
      481 cccgatacgt tcgaccgcga ccaccaggcg cccgacctca gcagtcgaca tcgatcacaa
      541 cggcacctgc gccaacgcat ccaaccagcc gcaatagcag caaccgcctc aacggcagga
      601 tgctgataat cggtggtgac agcgctcatg aaaacaaacc taccgagaac caccgacaca
      661 accgccacgc cagaagtacc tgtggattaa gagaaatgag catgtgaaga ggggatcatc
      721 ggtgtcgtcc gcgcagcacc tgcccgcccc cgcaatctcc cgcaactaca atcaagagat
      781 gacaggttca gggacttcgc cgtcgctcta tgagtgggct ggcggtgagc ccgccattgc
      841 gcgcatgatc aatgcgttct acgaccgggt cgagaacgac gaacttctta gtccattctt
      901 tccaggtggc gttgccagca agcaccgtca gaacgtcacc acctggtggt gcgaggtctt
      961 cggtggccca gccgactaca cgaagtcact tggcggctac cacacgatgc tcgagcacca
     1021 cctcgagctc gacatcaccc aagaacagcg cttccggttc gcgttactca tgagcctcgc
     1081 cgctgacgac gcggagctcc cgagcgatcc ggagtttcgg gccgcgcttg tcggttacgt
     1141 ggaatggggc acccggctgg cgatgcacaa ctcgcggcca ggggccatcg tcgtggaaga
     1201 agcgccggta ccccggtggg gatggggcgt cgcgcctccg tacaaccccg gtggctcttg
     1261 atgcggccat gaccccgact cagctccggg cctactccgc cgtggtccgg catggttcgg
     1321 tgaaagcggc tgcggctgag atcggggtga ccgaggcggc catctccttg cacctcgggc
     1381 agttgcgcaa ggaactcgac gaccagttgt tcacgcgcac agctgccggt ctcgcgttca
     1441 cgcccggcgg tcttcggctt gccagccggg cggccgaaat gctcggtctg caggcgcaga
     1501 ccattcgcga ggtgaatcaa gctggccacg gccggcgcct catgaggctc gccaccacca
     1561 gtctcttcgc cgagcacgcc gcacccgggc tgatcgagct gtttcttgcg cgtgccgacg
     1621 atttcgacgt cgaactcagc gtgcggccgc cttcgcagtt cgacagcctg ctggggtcgc
     1681 gcgcagtcga tgttgcgatt ggtccgcgga tgtcgagtgc cggcaacgcg ctggtgtgca
     1741 agccgttcct caactatcac gtcgtcgcgg ttgtgggcgt cggtcacccg ctcgccacca
     1801 gcgcgtctcg tccggcgctc ctacgggagc agacctggct gctcgggcca tcagccgcag
     1861 gcgaggacgg cgtggtgccg gtcatgcttc gccagctcga cattcctgag gaacgccagc
     1921 gaatctttca aagtcacgca gctgcgctcg aagaggcaaa gcgaaacaac ggcattgcgc
     1981 tcgcggtctc gttcgctgtt gctgctgatg tcgcgaacgg acggctcgtt gttatcaagg
     2041 gctccgggtt acaggccgag ggcggttggg cgacgatgac tctgccggag aatgtcaccg
     2101 tgcctgctgc ggctgagctg acccgattca tctctacgcc gcgcgcgatc caagcgatga
     2161 tgaagggctc cggggtgcac atcggccact tccggccgtc cgtgcacgtc acgctctgga
     2221 agtagtcgaa ggcacaaacg ggcggacgcc gtctggtcga ggccgtcgtc cggcgcgcgc
     2281 gacagtgtcg aaagcatcgc cgagcgcgcg caagtcgtgc gcgggcaaca gatcgtcgca
     2341 gtagggcagt gccgccgcca tcgcgccgac caacggtgcg aaccccgatg cggctacccg
     2401 cgggttcatc caaatcactc gatgcgctcg ccgccgcagc cgggccatcg atgcggcgag
     2461 ctcgtgtgga gggtcactgt cccatccgtc ggacgcgatc acgactactg cgccccgcaa
     2521 aacgcctcca tgtcgcgatc ggagaagtgc atcgagattc gatgcgatcc gggtgccgcc
     2581 aaagcgatca accaccgctg cagtcgcttg ctcgatcgcg acatcgacgg atcgatgagt
     2641 cagcacagat gtgagccggg taagcccggt cgcgaaaacg aacacctcgg cgtttgccga
     2701 caacaccgcc gcccgcatga ggtgcaagta cgtggtcgca tgtgcctgca tcgactggct
     2761 gacgtcgcag accatgacta cccgacgcgg cacgtagcgc ggcgcgctgc gcactacatg
     2821 caccgcctcc cagccactgc ggcgcgagcg caccatcgtc gcgcgcaaat tgacgtagcg
     2881 cccatgcgcg tgcggctgta ctcgccgggt gcgacgcgag ggccagtcgc ggaacgcgct
     2941 ctccaaccac acgccgagca gttcaagctc gtcggcgctg aggtcctcga atggcgtcag
     3001 cgctatgccg acaagattgc tcggcagtcg ctctggcata ccgagcggcg actcatcgtc
     3061 atcgacgacg ccgacgatgg ccggcagggt ggcccaaggc agcccaccgc cgtcctgatg
     3121 cagcacgccg tcgtcacccg acgctggggc gaaccggtca tcaccggcgc gcgaccgcgc
     3181 aggtgcggat cgccgtgcgt gcggatcaag atgcaggccc gcatcgtcga agaccgcggc
     3241 aaatacccgg tcgaatgcct cgatgtcact gtgtcggtgc gtgaggctga tcctggccaa
     3301 ccagtacaac gcagagcggg tggacggcgg ttgtgcggcg agagcccgcg caaaggtgct
     3361 cgcggcgggc aggcccacgt cgaccccggc cgatcgcaat cgatcggcga aggccaccgc
     3421 gaatgcggcc cgatcgacac cgctgagcac agtgacgtga cgtgccgtgg cggctgactc
     3481 gagaaggccg ccgttatcgg acgatgacat agatgatcac gccgacaagg atgatgcctg
     3541 cgagcaccgg cagcgcccgc ttcgccaccg cgccaccggc gaggtgggtg aggtcgacgg
     3601 gctcgggggc cgcggacgga cggaaggccg tcggggcagc aattgtcgcg gctcgtggcg
     3661 caccgtctgc cgtttgttct gcagcgggct ttgtcccgct cggcacctcg ggggccgacg
     3721 cgagcagctg ctcgagcgac tccgcgaact ggcctagcag cttgctcgaa acctccgcga
     3781 tcatcccgct gccgaactgt gcgagcttgc cgctgatctt catgtcagtc tcgaccatga
     3841 ctatcgtgcg cgttgcgccg tcgcttcgaa gttgcgcagt gatcgtcgct gaggcattgc
     3901 ccgatccacg cgagtcacgg cccttcgcgt cgatgatcgc gtggtaggtc gcttcatcct
     3961 tctcgacgaa ggaagccgtt ccggcgaact cgctgatgac cggtccgacc ttcaccttca
     4021 ccgtgccgag atacgcgtcg ccctcacggc cggtgagttg ggcacccggc atgcatggag
     4081 cgattcgctc cagatcggtg agcgcatgcc atgcctcgtc gatggggaca ttcacgctga
     4141 actgattgtc gatcttcatc ggatcctctt cgcgcggggg tgggggatca ttcggaggta
     4201 gccgcgggcg cgctgatctc aaccagcgcg cctagcatcg tgtcgcgatc gtcgggcgtc
     4261 ttggcgatgg cgccgagggt gctcacgagt gttgcccgca ccagctcggc ggcaccaagc
     4321 gcgctcaacg cggaaagcca gtcgatggtc tcggccattc ccggcggctt gtcgagatcc
     4381 aacgtccgca cccgcccgac gaactgggtc gcgctttgga tcagtgcttc gcttgcggcc
     4441 ggcactccac gccgcaaaat ttcggcggcg cgggtcggag tggggaagtc gatccagtgg
     4501 tacagacatc ggcgccggag cgcatcatgc aattcacggc tccggtttga ggtcaagacc
     4561 acgatcggcg ggtgcacggc cgtgaacgta cccagttcag ggatcgtcac tgacgattct
     4621 ccgagaaact cgaaaagcaa cgcttcgaac tcgtcgtccg ctcgatcgat ttcatcgatc
     4681 aggaggactg gtggagttgc accgcgatgc ctcaccgcct gcaggattgg ccgttcgagc
     4741 aggaatgcct cggtgaacag gtcggcatcg gtgatggctt ctgcgctcgc ctctgcgagt
     4801 cgaatggcga gcagctgccg ctggtagttc cactcgtaca gggcctcgcc gacggttagt
     4861 ccctcgtaac actgaagcct gatcagcggg acgtcgaggg ctcgagcaag tgacttggct
     4921 gccgtagtct tgccgactcc cgg
//
LOCUS       DATCBJ010000249         3225 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_27892, whole genome shotgun sequence.
ACCESSION   DATCBJ010000249 DATCBJ010000000
VERSION     DATCBJ010000249.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 3225)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3225)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3225
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_27892"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..880
                     /gene="ccsB"
                     /locus_tag="VII50_05150"
     CDS             <1..880
                     /gene="ccsB"
                     /locus_tag="VII50_05150"
                     /inference="COORDINATES: protein motif:HMM:TIGR03144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="c-type cytochrome biogenesis protein CcsB"
                     /protein_id="HEY5050265.1"
                     /translation="RLDVRVPALVGGASRASGAAATPASAPPAGPPRARSSFVSAHAG
                     QFAIAATVVGWLLHLGAIVSRGLSVHRLPWGNMYEFTLALTFTAVTAFLVLMMRQPAR
                     YLGVFVMTPVVLALGIAVLVLYVAPAPLVPALHSYWLAIHVTAAIIASGVFTVATAAT
                     TMFLVADHYETATAAGRPVRFSGLARIMPSPAVLDRTAYRAYAFAFPVWTFAVIAGAI
                     WAEYAWGHYWQWDPKETWAFITWVIYAGYLHARATAGWRGRKAAYIAMLGFTSILFNF
                     FIVNIFISGMHSYAGV"
     gene            945..1574
                     /locus_tag="VII50_05155"
     CDS             945..1574
                     /locus_tag="VII50_05155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5050266.1"
                     /translation="MIAAAIRLIQHPVYSSSSPRVRVTVVGGCPQSLGKAIDVSSPGP
                     NRWSELFHYSRLARSGAIGGLVCQYGLPQTTANQGLPTDGPSNSTAVVEQGTEDATAL
                     ANMPPQSPPILQSHAVLTPDQARAVSLAAFTQSTLHPSGTFHCPSSTDSSAIIVVLSY
                     PTGADIDIWWNDTGCQNADNGHVEVYFYGVGLSGGDFTGAVEQVFPAAP"
     gene            1623..2360
                     /locus_tag="VII50_05160"
     CDS             1623..2360
                     /locus_tag="VII50_05160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5050267.1"
                     /translation="MHRSSMAIATAVLGVAGLVACASARIATTNGSLASPTPSGTARG
                     GAYSSPLPGPAFSAPPGQHLRVTVAQGCPATMPWAVTDVENPADPARTNALAPTGATS
                     GLICRYATDNVLYFSPGANTEVDTPAPLPVPNLRASVVLTPAQAATLSAAAAVASVAP
                     ATGSYSCPSGDPGHVALIVLGYPDRADVDLWYSDTGCQDVDNGYASDFLLAGGDFGGA
                     VDALVPRPSSGPATPSASTRATPSPSG"
     gene            2409..>3225
                     /locus_tag="VII50_05165"
     CDS             2409..>3225
                     /locus_tag="VII50_05165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HEY5050268.1"
                     /translation="MSFADLPDFLERLEKDGDLVRVKAQVDPHLEITEIVDRVVRAGG
                     PALLFENVKGSRLPLAMNVFGTTARMAKALGVDDFDEIGARIGDLLKPELPKGFAGLK
                     DAMSKLGQLRDVPPKTVRKGDCQDVVLKGDKVDLMSLPALHTWPDDGGSYFNLGLTHT
                     KHPETGQRNLGLYRLQRHDARTVAMHWQIHKDSNAHHAVAERRGERLPVAIAFGCDPV
                     VTYAASAPLPSDIDEYLFAGFLRQERVEMVDCMTVPLQVPADAQVVLEGWLEPG"
BASE COUNT          492 a         1097 c         1040 g          596 t
ORIGIN      
        1 ccgcctcgac gtccgcgtgc ctgctcttgt cggaggagcg tcccgagcga gcggtgctgc
       61 cgcgacgccc gcctcggcgc cgccagctgg cccgccgcgc gcgcgaagtt cgttcgtttc
      121 ggcgcatgcc ggtcagttcg ctatcgcggc caccgtcgtg gggtggttgc tgcaccttgg
      181 ggcgatcgtt agccgcgggc tgtcggtgca caggttgccc tggggcaaca tgtacgagtt
      241 cacgctggcg ctgacgttca cggccgtgac cgcgttcttg gtgctcatga tgcggcagcc
      301 ggcgcgttac ctcggcgtct tcgtcatgac acccgtcgtg ctggccctgg gcatcgccgt
      361 gctggtgctc tatgtcgctc cggcgccgct ggttcccgcc ctgcattcgt actggctggc
      421 gattcacgtg accgcagcca tcatcgcgtc gggagtcttt acggtcgcga cagccgcaac
      481 aaccatgttc cttgtcgctg atcactacga gacggcgaca gccgctgggc gcccggtgcg
      541 gttctcggga cttgccagga tcatgccgtc gccggctgtt ctcgaccgca cggcctaccg
      601 cgcctacgcg ttcgcgttcc cggtctggac gttcgccgtg atcgccggtg cgatctgggc
      661 tgagtatgcg tggggacact actggcagtg ggaccctaag gagacatggg cgttcattac
      721 ctgggtcatc tacgcgggct atctgcacgc gcgggcgacg gctggctggc gcggacgaaa
      781 ggccgcctat atcgcgatgc tcggattcac gtcgatcctg ttcaacttct tcatcgtgaa
      841 catcttcatc agcggtatgc actcctacgc cggcgtctag gccggggacg cgcccagctt
      901 ggcggcgtgc tgccttcacc gccaccatcg tcgtcgtcat cgccgtgatt gcggccgcca
      961 tcaggttgat ccagcacccg gtctactctt cgagctcgcc gcgggtcagg gttactgtcg
     1021 tcggcgggtg cccgcagtcg ctcggcaagg cgatcgacgt ctccagccca ggaccgaatc
     1081 ggtggagcga gctgttccac tacagccggc tcgctcgcag cggtgcgatc gggggcctgg
     1141 tgtgccagta cggcctgcca cagacaactg caaaccaggg gctgcctacc gacgggccgt
     1201 cgaattcgac cgcggttgtt gagcagggta ccgaagacgc caccgcgctg gcgaacatgc
     1261 cacctcagtc gcctccgatc ctgcagtccc atgctgtcct cacccccgac caagcccgtg
     1321 ccgtgtcgct ggccgcattc acccagagca ccctgcaccc gagtggcacc tttcattgcc
     1381 catcgtcgac agactccagc gcgatcatcg tcgtgctgag ctatcccacc ggcgctgaca
     1441 tcgatatctg gtggaacgac actgggtgcc agaacgccga caacgggcac gtcgaggttt
     1501 acttctacgg cgtcgggttg tccggcggcg acttcaccgg agccgttgag caggtgttcc
     1561 ccgcggcacc atgaaaccgg tgtcggtggc tgcgcgtctg atgctcaaca aggaggttgc
     1621 gcatgcaccg ctcgagcatg gctatcgcaa cagccgtgct cggcgtcgca ggcttggtcg
     1681 catgtgcgag tgcgcgcatc gccacgacga acggatcgct tgcgtctcca acgccatccg
     1741 ggacggcgag gggtggcgcc tactcgtcgc cgcttcccgg tccagctttc tccgccccac
     1801 ctggtcagca tctgcgggtc actgtcgcgc agggctgccc ggcgacgatg ccctgggcgg
     1861 tcactgatgt cgagaaccct gccgatccgg cccgcacgaa tgcacttgca ccaaccggtg
     1921 cgacaagcgg gttgatctgc cgctacgcaa ccgacaacgt tctgtacttc tctcccggcg
     1981 cgaacaccga ggtcgatacc ccagcgccgt tgccggtgcc gaacctgcgc gcatcggtcg
     2041 tgctcacccc ggcacaggcc gcgacgttgt cggcggcggc tgcagtcgcc agcgtcgcgc
     2101 cagcgaccgg ctcctacagc tgcccaagcg gtgaccctgg gcacgtcgca ttgatcgtgc
     2161 ttggctatcc cgatcgcgcc gacgtcgatc tctggtacag cgacacgggt tgccaggatg
     2221 tcgacaacgg atacgcatcc gatttcctgc tcgccggcgg agacttcggc ggggcggttg
     2281 acgccctggt tccccggccg tcgtcggggc ccgccacgcc gtcggcgtcc acccgcgcca
     2341 cgccctcgcc gtccggctga agttggcctg aacggcggca acccgaggcg accggcgata
     2401 cttgatctat gagcttcgcc gatctgcccg acttcctcga gcgcctggag aaggatggcg
     2461 acctggtgcg cgtcaaggcg caggtcgacc cgcacctgga gatcaccgag atcgtcgacc
     2521 gggtcgtgcg cgctggtggt cctgcgttgc tgttcgagaa cgtcaaaggc tccaggctgc
     2581 cgctagcaat gaacgtgttc ggcaccaccg cccggatggc caaggctctt ggtgtcgacg
     2641 actttgatga gatcggcgcg cgcatcggcg acttgctgaa gcccgagttg ccgaaaggct
     2701 tcgccggtct caaggatgcg atgagcaagc tgggccagct ccgcgacgtc ccgccgaaga
     2761 cagtgcgaaa aggcgattgc caggacgtcg tgctgaaggg cgacaaggtc gacctgatgt
     2821 ccttgcccgc gttgcacacc tggcccgacg acggcggttc gtacttcaac ctcgggctca
     2881 cccacacgaa gcacccggag accgggcagc gcaacctggg gctctatcgg ctgcagcggc
     2941 acgacgcacg cacggtggcc atgcactggc agatccacaa ggactccaac gcccatcacg
     3001 ccgtcgccga gcggcggggc gagcgactgc cggtcgcgat cgctttcggc tgcgaccccg
     3061 tggtcaccta cgcggcgtcc gccccgttgc ccagtgacat cgacgagtat ctgttcgcgg
     3121 gtttcttgcg gcaggagcga gtcgagatgg tcgactgcat gacagtgcca ctgcaggtgc
     3181 ctgcggatgc gcaagtcgtt ctcgaaggtt ggctcgagcc gggcg
//
LOCUS       DATCBJ010000250         2694 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_384757, whole genome shotgun sequence.
ACCESSION   DATCBJ010000250 DATCBJ010000000
VERSION     DATCBJ010000250.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2694)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2694)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2694
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_384757"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1875
                     /locus_tag="VII50_05170"
     CDS             <1..1875
                     /locus_tag="VII50_05170"
                     /inference="COORDINATES: protein motif:HMM:NF024947.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SbcC/MukB-like Walker B domain-containing
                     protein"
                     /protein_id="HEY5050269.1"
                     /translation="ERRRRLTEIDDEIARTTVALDTARRDGQTATAQRIALETWLRQM
                     PSSTVLREARTTLSAASGDLGRAAVDANAARAHAARARSVLAELRHVLDDLARVNALP
                     TDRTGLDARAEQLSQLTRQRDRLAANVSRLPATLARWSDDFASRQADSLVAQQTVRDA
                     EAAESLAAAAEAEHVLLLHALGAGVAALHEKLAMQRQLAADAQSRADASESVLRELGE
                     KRGAATSDIRIAESHFADVAVSVAAVATRLVHLLEVPGLFSAGLGRDPTAAETAVLGT
                     LRTPEHAAVDRLAADVEQVVTRYAEEAAPAKNVGESDIYSEWQVVASGPAAETQPRVI
                     EVGGVLAVLAQDDAGEHPLPILAVRLAARVAADRELLSNRERALFEEHLLGELGEALR
                     ARRQEATDLVAAMNRLLVGVRTSQGIGVRLDWELRDDVSVEVRDSVALLGRSLGALLP
                     DERTRLRDALHTMIESSRSDSPELSYAEHLGRALDYRRWSAFRVRLSRPEAPGWTTLT
                     RRTPLSQGEQKVVCYLPLFAAAAAHFTSVAGAAPYAPRFVLLDDAFPKIDVRTHPLLF
                     GLLVDLDLDFVVTSERLWGDHATVPSLAIYEALRSPTERGIAQYRHVWDGQRLTAVGA
                     "
     gene            1872..>2694
                     /locus_tag="VII50_05175"
     CDS             1872..>2694
                     /locus_tag="VII50_05175"
                     /inference="COORDINATES: protein motif:HMM:NF023224.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02679 domain-containing protein"
                     /protein_id="HEY5050270.1"
                     /translation="MTSSALPAWLADPALSSVWSQLRAPLERRGNDVTGGVRMRNLDR
                     EARHALAGLLGRSIVTDTVSVDLAELDMTLRGRAGRGLRDVVATVTGEPLRDRPAERL
                     AVSRRREELLELLQSRVEGSWALRARPWVPEWLAGVRASGMLTRIGDVRVTVASAVAV
                     LERVLDTDSMSRGDLAAELAHDAHALDDGTAVGALVLRALAAAEGLRAVPPDARGRRE
                     LWSRFGVATDLVSTTVLTLGLRPQAHGSRERRLCDAADDGDPIHLTGRDLQGLRLR"
BASE COUNT          430 a          861 c          928 g          475 t
ORIGIN      
        1 gagcggcgtc gccgactgac cgagatcgat gatgagatcg cacgcaccac ggtcgcactc
       61 gataccgcac gacgcgatgg ccagaccgcg acggcgcaac gtatcgcgct cgagacgtgg
      121 cttcgacaga tgccctcgtc gaccgttcta cgtgaggccc ggaccacgct ctccgcggcc
      181 tccggtgacc ttgggcgtgc ggcggtcgat gcaaacgccg cgcgcgccca cgccgcacga
      241 gcacgatcgg tgctggccga acttcggcac gtgcttgacg accttgcccg agtgaacgct
      301 ttgcccaccg accgaacggg cctcgacgcc cgcgccgagc agttgagtca gctgacgaga
      361 cagcgcgacc ggttggccgc gaacgttagc cggctgcctg cgacgctggc caggtggagc
      421 gacgacttcg ccagccgcca ggccgactcg cttgtcgcac agcagaccgt acgtgacgcc
      481 gaggccgccg aaagtctcgc ggcagccgcc gaagccgaac acgtcttgct gctgcacgca
      541 ctcggtgccg gcgtcgctgc cctgcacgaa aagctggcga tgcaacgaca gcttgcagcc
      601 gacgcgcagt cacgggcgga tgcgtccgag agcgtgcttc gggaactggg agaaaagcgc
      661 ggtgccgcga cgtccgacat caggatcgcc gaatcccact tcgccgacgt tgccgtgtcc
      721 gtggcagccg tagccacgcg gttggtgcat ttgctcgaag tgccaggact gttttcggcc
      781 ggcctaggtc gcgatccgac tgctgccgag accgccgtac tcggtacgct ccgcacaccc
      841 gagcatgccg cggtcgaccg attggccgca gacgtcgaac aagtcgtgac tcgttacgct
      901 gaggaggctg cgcccgcgaa gaatgttggc gaaagcgaca tttacagcga gtggcaggtc
      961 gtggcatcag gaccggctgc cgagacacag ccgcgcgtca tcgaggtcgg tggcgtgctc
     1021 gctgtccttg cccaggacga tgccggggag catccgttgc caatcctggc ggtgcgactg
     1081 gcagccaggg tggctgcgga ccgagagttg ttgagcaatc gtgagcgtgc cctgttcgag
     1141 gagcatctgc tcggcgaact cggcgaggca ttgcgagctc gccgacagga ggctaccgat
     1201 cttgtcgccg cgatgaatcg gctgctggtc ggtgtgcgga cgtcgcaggg catcggcgta
     1261 cgcctcgatt gggagctacg cgacgacgtc agtgtggagg ttcgtgattc cgttgctctg
     1321 ctcggccgct cgctcggggc gttgttgccc gacgagcgga caaggcttcg cgacgctctg
     1381 cacaccatga tcgagtcgtc tcggagtgac agtcctgagt tgagttacgc cgagcatctc
     1441 gggcgagcat tggactaccg gcgttggtcg gcgttcaggg tgaggctgtc gaggcctgag
     1501 gcaccgggct ggaccacctt gaccaggcgg accccgctgt cacaaggcga gcagaaggtc
     1561 gtgtgctacc tgccgctttt tgctgcagca gcagcacatt tcacatcggt ggccggtgcc
     1621 gcgccgtacg ccccacggtt cgtcctcctc gacgatgcat ttccgaagat cgacgtgcgc
     1681 acccatccgc tgttgttcgg cctgctcgtc gacctcgacc tcgatttcgt tgtcacgagc
     1741 gaacgactct ggggcgatca tgcgacggtt ccgtccctcg cgatctacga ggcgctgcgt
     1801 tcgccgaccg aacgcggcat cgcgcaatac cggcacgtgt gggacggtca acgtcttacc
     1861 gcggtcgggg catgacgtcg tccgcactgc ctgcgtggct tgcagatccc gcgctgtcga
     1921 gcgtgtggtc gcagttgcgc gcgccgctcg aacgtcgggg caacgacgtg accggtgggg
     1981 tgcggatgcg caaccttgac cgggaggcgc ggcacgcact tgcgggcctg ctcggtaggt
     2041 cgatcgttac cgacactgtg tccgtcgacc tagccgaact cgatatgaca ctacgcggcc
     2101 gcgctggtcg tgggctgcgc gacgtggtgg cgacggtaac cggcgaaccg ctacgcgacc
     2161 gccctgccga acggttggca gtgtcacgac gacgtgaaga actcctggaa ctgttgcaat
     2221 ctcgggttga gggttcgtgg gcccttcgcg ctaggccgtg ggtgccggag tggctggctg
     2281 gtgtccgcgc gagcgggatg ttgacgcgta tcggtgacgt acgcgtgacg gtggcatctg
     2341 cggtggcggt gctcgaacgg gtgctcgaca ccgactcgat gtcgcggggc gacctcgccg
     2401 cggagcttgc acacgacgcg cacgccctcg acgacgggac agccgtcggg gcgctcgtac
     2461 tacgtgcgct cgccgcagcc gaaggactgc gggccgtacc gccagatgca cgcggccggc
     2521 gtgagctgtg gtctcgattc ggcgtggcga ctgaccttgt ctcgacgacc gtgctcacat
     2581 tgggtttgcg cccgcaagcg cacgggtcgc gcgaacggcg attgtgtgac gccgcagacg
     2641 atggtgatcc cattcactta accggccgcg acttgcaagg tcttcggctt cggc
//
LOCUS       DATCBJ010000251         2561 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_447240, whole genome shotgun sequence.
ACCESSION   DATCBJ010000251 DATCBJ010000000
VERSION     DATCBJ010000251.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2561)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2561)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2561
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_447240"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..572
                     /locus_tag="VII50_05180"
     CDS             <1..572
                     /locus_tag="VII50_05180"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HEY5050271.1"
                     /translation="RLRPVWRSVLAVPETPNVSLRPRTDDDLDLLFEIAADLDTWEER
                     NPQAPRPLTRDRFDARLARGVDSDAPGDAVSLVIDLDGVAVGAASLFDFDFLARHAEA
                     GVSLVQHARGRGIGTAAVIQLVEFGFVRHNLRRIHLQAVASNVAAIRAYEKAGFVIEG
                     RQRQHAWVRGTYEDIVRMGILRSERTTST"
     gene            complement(627..1463)
                     /locus_tag="VII50_05185"
     CDS             complement(627..1463)
                     /locus_tag="VII50_05185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5050272.1"
                     /translation="MRDDGWVIHSAPPADLLADPTVGAAGGFVQRVGEGAALDAAARW
                     VTGTDARSRQVGFAVLGFVGLDDDDARRALLEAARLAAGDPYPDVRMSVAAAIGNLSD
                     CADAHHLLLSMVDDGDDAVLAQVVGGLAITASDSTTIAEPWVQSLVTLLRHDSALVRD
                     WAAFALGTQSDVDGPEVRDELLRIAETDADGEDVYPAAEAAMGLARRQDSRVQPIIER
                     RLVDVAVGRLWLEAAAELADPRLHPALVRLREEVDPEPCTSWDDSLDLAIQTCSPRLD
                     RS"
     gene            1887..>2561
                     /locus_tag="VII50_05190"
     CDS             1887..>2561
                     /locus_tag="VII50_05190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5050273.1"
                     /translation="MVAGLTLGYLVMLATKPGGAVLVKQISDVTTAAAAAAACVACAL
                     AAGRCAASMRVFWWVLTAAMGAWTAGESAWTWYEVVLRVQVPTPSWADVGYLVAPVLC
                     VAAFACHPAIRNRHRWGVLPILDGVAVASALLFVSWTLVLGPLWSHSGGVSVGALIAV
                     AYPFSDVVILVLVVLALQNLPRGNRAVISLLLGGLVCMALSDTVYTYLTQKNLYSSGD
                     LIDAGWF"
BASE COUNT          387 a          854 c          857 g          463 t
ORIGIN      
        1 agcgcctaag gcccgtttgg cgcagtgtcc tcgccgtgcc tgagacgccc aatgtgtcgt
       61 tgcgcccgcg caccgacgac gaccttgatc tgctgttcga gatcgccgct gacctcgaca
      121 cctgggaaga gcggaatccg caggcgccgc ggccgttgac cagggaccgt ttcgatgctc
      181 ggctggcgcg cggcgtcgac agcgatgctc ccggtgacgc cgtgtcgttg gtgattgatc
      241 tggacggtgt tgctgtgggc gccgcgtcgc tgttcgattt tgacttcctg gcccgccacg
      301 ccgaggccgg ggtcagtttg gtccagcacg cgcggggccg cggtattggg acggcggctg
      361 ttatccaact cgtcgagttc gggttcgtgc gtcacaacct gcgtcggatt caccttcaag
      421 cggtcgcctc gaacgtcgcc gcgattcgcg cgtatgagaa ggcaggtttc gtgatcgagg
      481 gacggcagcg tcagcatgcg tgggttcgag gcacgtacga ggacatcgtg cgcatgggga
      541 tcctgcgctc ggagcggacc acttcgacct gatccacatc cgccaccact accgcgctct
      601 catggcgcgg cgaaggtcgt ttgctgtcag ctccggtcga gccgtgggct gcaagtttgg
      661 attgccaggt caagagagtc gtcccatgag gtgcacggct cgggatctac ctcctcgcgt
      721 aaccgaacaa gcgcggggtg aagtcgtggg tctgcgagtt cggctgcggc ctccaaccac
      781 agcctgccga cagccacatc aacgaggcgt cgctcgatga tgggttggac gcgggagtct
      841 tggcgacgag ccagtcccat ggcggcctcg gcggcagggt agacatcctc gccgtcggcg
      901 tcggtctcag cgatccgcaa cagctcatcg cgcacctcag ggccgtccac gtcggattgg
      961 gtgccgagcg caaacgcggc ccagtcccgc acgagcgcac tgtcgtgacg caaaagcgtc
     1021 accagcgact ggacccacgg ctcggcgata gttgtggagt cggacgccgt gatagcgagg
     1081 ccgccgacga cctgagcgag caccgcgtcg tcgccgtcat cgaccatgct cagcagcaga
     1141 tggtgcgcgt ccgcgcaatc ggacaagttg ccgatcgcgg ccgccaccga catccgcaca
     1201 tccggataag ggtcgccggc cgcgagccgc gcagcttcca gcagcgcgcg gcgcgcatcg
     1261 tcgtcgtcca gcccgacaaa cccaaggacg gcgaaaccaa cttgccggga ccgtgcatca
     1321 gtaccagtga cccaccgagc ggccgcatca agtgctgcac cctcgccgac ccgctgaaca
     1381 aagccaccgg cggcgccgac agtcgggtcc gccagcaagt ccgccggcgg cgctgagtgg
     1441 atcacccacc catcgtcgcg cacgaccgga cgcttccaac ggaaacatcc tcaactgccg
     1501 ctgacacggt ctggaagcca ctgctgtcgg tcacgccaga tgcgttaggg cgctgggcgg
     1561 gtacttcgtc gcaagtccgc acgcgtgatc actacaccga tcaggctgcg tgcgcgccac
     1621 gcgagccact actggcatca ccaccacgga ccggcgtggg ggacgcggta gtgagatcgg
     1681 ggcgttcggc cgtaatggtg catcggggct acgcctgagg gtcctttggc acctcaccgg
     1741 ctcgggtcct gctgtcgatg cctcgtttcg tggacgaagc gtgggatcgg gcagcgggtg
     1801 ccgccggaac acgttccggt gcgtcaccgc tgcaagcgcc ctcgtccacg gtggtgccag
     1861 gatccgcgag gtctcgggtg gcgcgcgtgg ttgctggtct gaccctcggc tacctcgtca
     1921 tgttggccac caagccaggt ggcgccgtct tagtgaagca gatcagcgat gtcaccaccg
     1981 ccgcggcggc cgccgccgcc tgcgtcgcct gcgcactggc cgcaggccga tgcgcggcct
     2041 cgatgcgcgt gttctggtgg gtgctcaccg ccgccatggg tgcgtggaca gccggggagt
     2101 cggcctggac ctggtatgag gtggtcttgc gcgtgcaggt gccgaccccg tcttgggccg
     2161 acgtcggcta cctggtggcc cctgtgttgt gcgtggccgc gttcgcctgc caccctgcca
     2221 tccgtaaccg gcacagatgg ggcgtgctgc cgattctgga cggcgtcgcc gttgcgagcg
     2281 cactgctgtt cgtcagctgg acgttggtcc ttggcccgct gtggtcccac tccggtggcg
     2341 tatcggtggg agccctcatc gccgtcgcct atcccttcag tgacgttgtc atcctcgtcc
     2401 tcgtcgtgct ggccctgcag aatctgccgc ggggcaaccg ggcggtgatc tcgctgctgc
     2461 tcggcggcct cgtgtgcatg gccctctccg acaccgtgta cacctatctc acgcagaaaa
     2521 acctctactc gtccggcgac ttgatcgacg ccgggtggtt t
//
LOCUS       DATCBJ010000252         6045 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_218051, whole genome shotgun sequence.
ACCESSION   DATCBJ010000252 DATCBJ010000000
VERSION     DATCBJ010000252.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 6045)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6045)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6045
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_218051"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(76..1500)
                     /locus_tag="VII50_05195"
     CDS             complement(76..1500)
                     /locus_tag="VII50_05195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HEY5050274.1"
                     /translation="MAALHDLSALEQAAAVRRREVSPSELVEHYLDRIATWSDTVGAY
                     VTVTAELAHEQARQAEKTLREADADELPPLLGVPIAIKDLTDVAGIRCTLGSAAFAER
                     VATVDAHVVTLLRKAGTVILGKSNAAEFGAPCYTEPDVAPPARTPWDLSRSAGGSSGG
                     AGAAVAAGLAPFAQGNDGGGSVRIPASVCGLVGLKVSRGRVSNGPVSGDVSGLAWHGP
                     LTRTVRDAAAWLDATALPMPGDPHWAPPLPPGDSFLATVDREPGQLRIARYADPVIVD
                     DAVDPSCLAAYDDATKLLLELGHRVDDVPAPAGAGVVPAFETVWSVLSSLTPVPAADE
                     AKLRPLTRWLRERGGRVTGHEFAVAIATLQAAARRILVDLVEYDAVLTPTLAMPPVAV
                     GALRDDDDPARDFDNQKRFTPFTSVANLTGQPAITLPLGATSAGLPIGVMLAGRPAGE
                     AGLLALAGQLEVARPWFDRHPECW"
     gene            complement(1525..2268)
                     /locus_tag="VII50_05200"
     CDS             complement(1525..2268)
                     /locus_tag="VII50_05200"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEY5050275.1"
                     /translation="MSTSSSAGDSTSAGTGPGAITPDGCAVDLYALVPARDEPAIIDA
                     ASPPNASILELGCGAGRVTHPLIALGHSVVAVDESPEMLAHVHGARTVVSRIEDLDLS
                     LRFDVVVLSSHLVNVPNDDQRRAFLATCARHVLETGCVVIQRHPVSWFDTLAPSRRSR
                     PAGDGVEPITFVLDGISRPAPDLVAATAEYLVGDRRWTQTFVARRLDDAQLNTALAQV
                     GLRLDDAYLSDDGSWLRARPIALMGAGGG"
     gene            complement(2265..2795)
                     /locus_tag="VII50_05205"
     CDS             complement(2265..2795)
                     /locus_tag="VII50_05205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HEY5050276.1"
                     /translation="MAYALVLNATYEPIGVVPDRRALLLVLAAKAISLEAGDVVVHSA
                     AAAVSVPAVVKLTRFVKIPYRNVVPLTRRGVFLRDGGRCAYCEAPATSLDHVVPRSRG
                     GTHTWDNVVSCCRRCNHVKADRTVTELGWRLRRAPRAPSGIAWRILGTGRSDPKWIPY
                     LDGDGFGWAVDQSASA"
     gene            2976..5072
                     /locus_tag="VII50_05210"
     CDS             2976..5072
                     /locus_tag="VII50_05210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HEY5050277.1"
                     /translation="MTKPTYPAARRLDLVEKLPAAAPTFDVPDPYRWLEDPATSDSVS
                     WLEAEDALANEHLAALAGRAQLRTRLTSLMAAGVVTAPAWRGERQFFMRRTADQEHAV
                     LITVDPDGTERILLDPMQLDPAGTTTLDAWQPSKEGELLAYQISEGGDEESILRVIDV
                     ATGEIVDGPIDRCRYSPVGWLPGAQAYYYVRRLPASQVPAGEEQFHRRVWLHRVGTDP
                     ETDVCVFGADFDKTTFFSASVSRDGHWLIVSASPGTAPRNDLWIADLTTCDPESPQLQ
                     ELQVGVDAQTSVHVGRDGRAYIYTDRDAPRGRLVVTTPADPGPQTWRDLLPEDPEAVL
                     EDFAILDGDELPRPVLMASWTRHAVSELSLHDLATGERIGEVLLPGLGSVGGLSERPD
                     GGPDCWFGYTDHTTPSSVFRYDALTGQTIAWASAPGTVEVPAVHARLVTYTSRDGTPV
                     RMIVVSTMAKPDHARPTILGGYGGFGASMTPGYSATMLAWVEAGGVYAVANLRGGGEE
                     GESWHRAGMRGNKQNVFDDFHAAADWLCDHGWTTPAQLGISGGSNGGLLVGAALTQAP
                     DKYAAVACSAPLLDMVRYEKFGFGQLWSEEYGSAEDPEELGWLFAYSPYHHIEGGTQY
                     PAVLFTVFDSDTRVDPLHARKMCAALQAATTASRPIIFRRESDVGHGARAVSRSVDLS
                     VDTLAFLAAHTGLVLG"
     gene            complement(5085..>6045)
                     /locus_tag="VII50_05215"
     CDS             complement(5085..>6045)
                     /locus_tag="VII50_05215"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEY5050278.1"
                     /translation="VRYKGGLEIWHPVTDIAGKPIVVRTFVSAALYDQGVFSAIVILV
                     LVGIAILAVAMVIADRLAQWTVRPVAALAATANRLSSGQLLARAPVNGPREVVEVGVA
                     MNRLAQRINELLDDEREQVADLSHRLRTPVAAVKLAAEALPDSPEAVRLSEHVDALER
                     TVGSVIREARRGVREQATPACDVVSVVKERVAFWSVLAEDQGRRVELRLPTVSRFVRV
                     SADDLGAAVDALLENVFAHTPEGTAMAVAVEIDRVDAWITISDEGPGIGPDSVDRGRS
                     AGGSTGLGLDIVRRTAEGSGGSFGVGASPAGGAAMMMRLGPAD"
BASE COUNT          992 a         2109 c         2057 g          887 t
ORIGIN      
        1 cgccacgcca gtcatgaaag aaagcggcaa cgccgtgggc tgcggcgagg tcggcaagga
       61 ccgggtcgag tgacctcacc agcactccgg atggcgatcg aaccagggcc gggctacctc
      121 gagttgaccc gcgagcgcga gcaatcccgc ctcgcccgct ggccggccgg cgagcatcac
      181 acctatcggc aaccccgcgg acgtcgcacc gagcggcagg gtgatcgccg gttgcccggt
      241 cagattggcg accgacgtga acggcgtaaa acgcttctgg ttgtcgaagt cgcgggcggg
      301 gtcgtcgtcg tcgcgcaagg caccgacggc aacgggcggc atcgccagcg tcggtgtgag
      361 aacggcgtcg tactcgacga ggtcgaccag aatccgccgc gctgccgcct gcaatgtcgc
      421 gatcgcgacc gcgaattcat ggccggtcac ccgaccgccg cgctcccgta gccacctggt
      481 caatggccgc agcttcgcct catccgccgc tggaaccgga gtcaggctcg agagaaccga
      541 ccacaccgtt tcgaacgccg gcaccacccc ggcgcctgcg ggtgcaggca cgtcatcgac
      601 gcgatggccc aactcgagga gcaacttggt ggcgtcgtcg taagcggcca gacaactcgg
      661 gtcgacggca tcgtcgacga tgaccgggtc ggcgtagcgc gcgatccgca gctgacccgg
      721 ttcgcggtcg actgtggcaa ggaagctatc tccgggcggc aacggcggcg cccaatgcgg
      781 gtcgcccggc atcggcaacg cggtcgcgtc gagccaggcg gccgcgtcac gcaccgtgcg
      841 ggtcagcggg ccgtgccagg caagaccgct gacgtcgccc gagaccgggc cgttgctgac
      901 ccggccgcgg cttaccttga gcccgacgag tccgcacacg ctcgcgggga ttcgaacgga
      961 gccgcctcca tcgttgccct gggcgaacgg ggcgagcccg gcggcaacag ccgcacccgc
     1021 cccgccgctg gatccacccg cagaccttga caggtcccac ggcgtgcgtg cagggggcgc
     1081 gacgtctggc tcggtgtagc agggcgcgcc gaactctgcg gcgttggact tgccgaggat
     1141 caccgtgccg gccttgcgca gcaaggtgac gacatgggcg tcgacggtcg ccacccgctc
     1201 tgcgaaggca gcagagccaa gtgtgcaacg gatgcccgcg acgtccgtta ggtctttgat
     1261 ggcaatcggg acgccgagca acggcggcaa ctcgtcggcg tccgcctcgc gcagcgtctt
     1321 ttctgcctgg cgggcctgct cgtgggcgag ctcggctgtc accgtgacgt aggcaccgac
     1381 ggtgtccgac caggtcgcga tgcggtcgag gtagtgctcg accagttcgg acggcgacac
     1441 ctcacgacgc cgcacggcag ccgcctgttc aagcgcggaa agatcgtgca acgcggccat
     1501 gccgggagcg tacgcgtgcc ctcgtcagcc gccgcccgcg cccatcagcg cgatcggccg
     1561 ggcccgcagc cagctgccgt cgtcgctcag atatgcgtcg tcaaggcgaa gaccgacttg
     1621 cgccaacgcc gtgttcagct gcgcgtcgtc aagccgccgg gcgacgaacg tctgggtcca
     1681 tcgccgatcg ccgacgaggt attcggcggt ggctgcgacg aggtccggcg cgggacgcga
     1741 gatgccgtcg aggacgaacg tgatgggttc gacgccgtcg ccggccggcc tggaacggcg
     1801 cgacggcgcc aacgtgtcga accagctgac cggatgccgt tggatcacga cgcaacctgt
     1861 ctcgagaacg tggcgcgcac aggtcgcgag gaacgcccgc cgctggtcgt cgttaggcac
     1921 gttcactagg tgagaggaga gcacgaccac gtcgaaacgc agcgaaaggt cgagatcctc
     1981 gatgcgggac accacggtgc gcgcgccgtg gacgtgcgcg agcatctcgg gcgactcatc
     2041 gaccgcgacg accgagtggc cgagcgcgat caacggatgg gtcacccggc cggcgccaca
     2101 ccccagctcg aggatcgacg cgttgggtgg cgacgccgcg tcgatgatcg ccggctcgtc
     2161 gcgggctggc acaagtgcgt agaggtcgac cgcgcagccg tctggagtga tcgcgccagg
     2221 cccagtaccc gcgctggtgc tgtcaccggc cgagctgcta gtgctcacgc gctagccgac
     2281 tggtcgaccg cccacccgaa gccgtcaccg tcgaggtagg ggatccactt cgggtcgctg
     2341 cgcccggtac cgaggatccg ccacgcgatc ccgctcgggg cgcgcggcgc acgccgaagc
     2401 cgccagccga gctcggtcac cgtgcgatct gccttgacgt ggttgcaccg ccggcagcac
     2461 gacacgacgt tgtcccacgt gtgggttcca ccgcggctac gcggcacgac gtgatcaagg
     2521 ctggtcgccg gcgcttcgca atacgcgcag cgcccgccat cgcgcagaaa caccccgcgc
     2581 cgggtcaacg gaacgacgtt gcggtacggg atcttcacga accgggtcag cttgaccacg
     2641 gccggtacgg aaaccgccgc cgccgcactg tgcacgacca cgtcgccggc ctcaagcgag
     2701 atggccttcg cggcaaggac cagcagcagg gcgcgacggt ccggcacgac gccgatcggt
     2761 tcgtacgtcg cgttgagaac tagcgcgtaa gccacgccgc cctcctccgc cgcctcactg
     2821 ttggttcacc ccagtgcttc ttaccgacag tctcgccggt cggaccgtcc cggcgccagc
     2881 tattcacgcc gcgcggcgcg cacttcgcta ttcacgccgc gcgcgccgcg gcgtgagaac
     2941 gaatcccgac cagcctcgtt ggttagggtc gcttcatgac gaagccgacg tatcccgccg
     3001 cgcgtcgcct cgacctggtg gagaagcttc ctgccgcggc gccgacgttc gacgtccccg
     3061 atccttaccg gtggctcgag gacccggcga cgtcggactc ggtcagctgg ctcgaagcgg
     3121 aagacgcgct cgccaacgaa cacctagccg cgctcgcggg ccgcgcgcag ttgcgcaccc
     3181 gcctcacgtc gctgatggcc gctggggtgg tgactgcacc tgcttggcgc ggagagcggc
     3241 agttcttcat gcggcgcacg gccgaccagg agcatgccgt cctgatcacc gtcgatccag
     3301 acggcaccga gcgcatactg ctcgacccga tgcagctcga cccggctggc accacgactc
     3361 tcgacgcgtg gcaaccgtcg aaggaaggcg agctgctcgc gtaccagatc tccgaaggag
     3421 gggacgagga gtcgatcctg cgggtgatcg acgtcgcgac cggcgagatc gtcgacggac
     3481 cgatcgatcg gtgccggtac tcccccgtcg gctggctgcc cggcgcgcag gcctactact
     3541 acgtgcggcg gttgccggcc tcgcaggtgc cagcgggtga ggagcagttc caccgacggg
     3601 tgtggttgca ccgggtcggg accgaccccg agacggacgt gtgcgtcttc ggcgccgact
     3661 tcgacaagac gacgttcttc tccgcgtcgg tcagccgcga cggccattgg ctcatcgtga
     3721 gtgcatcgcc gggcactgcc ccgcgcaacg atttgtggat cgccgacctc acgacgtgcg
     3781 acccggaatc cccgcagctg caagaactgc aggtcggcgt cgatgcacag acctcggtgc
     3841 acgtcggacg cgacggccgt gcctacatct acaccgaccg tgacgcgccg cgagggcgtc
     3901 tcgtggtgac gacgccggcc gacccgggcc cacagacgtg gcgcgatctg ttgccggaag
     3961 atcccgaggc agtgctcgaa gacttcgcga tattggacgg cgatgagttg ccgcgacccg
     4021 ttctgatggc gtcctggact cgtcatgcag tgagcgaact cagcctgcac gacctggcga
     4081 ccggcgagcg catcggcgag gtcctgctgc caggtcttgg ctccgtcggt ggattgtcgg
     4141 aacggcccga cggcggcccg gactgctggt tcggctatac cgaccacacg acgccgtcga
     4201 gtgtcttccg ctacgacgcg ctcaccggcc agaccattgc gtgggccagc gccccgggca
     4261 cggtcgaggt gcccgctgtg cacgcccgac tggtcaccta cacctctcgc gacggcacgc
     4321 cggtccggat gatcgtggta tcgacgatgg cgaagcctga ccatgctcgg ccgacgattc
     4381 tcggcggcta cgggggtttc ggtgcatcga tgacgccggg gtactcggcg accatgctgg
     4441 cgtgggtcga ggccggtggc gtgtacgccg tagcgaacct gcgcggcggt ggcgaagaag
     4501 gcgagagctg gcaccgcgcc ggcatgcgcg gcaacaagca gaacgtgttc gacgactttc
     4561 acgcagcagc ggactggctg tgcgaccacg ggtggacaac cccggcccag ctcggaatct
     4621 ccggcggcag caacggtggt ctgctggtcg gcgcggcgct tacccaggca ccggacaagt
     4681 acgcggcggt cgcctgttcg gcgccgctgc tcgacatggt gaggtacgag aagttcgggt
     4741 tcggccaact gtggagtgag gagtacggca gcgcggagga tcccgaggaa ctcggctggc
     4801 tcttcgccta ctccccgtac caccacatcg aaggcggcac ccagtaccca gcggtacttt
     4861 tcaccgtctt cgactcagac acccgcgtcg acccgttgca cgcgcggaag atgtgcgcgg
     4921 ctttgcaggc agcgacgacg gcgagcaggc cgatcatctt ccgccgcgag agcgacgtcg
     4981 ggcacggcgc gcgtgccgtg agtcgcagtg tcgacctgtc cgtcgacacg ctcgcgtttc
     5041 tcgccgcaca taccggcctg gtgctcggct aatcggcggg gcatctagtc agccggaccc
     5101 agccgcatca tcatggccgc gccgccggcg ggtgacgcac caaccccgaa gctgccaccc
     5161 gacccctcgg ccgtccgtcg cacgatgtcg agaccgaggc cggtcgagcc gccggcgctg
     5221 cgtccccggt cgacggaatc cggcccgatg ccaggacctt cgtcggagat cgtgatccag
     5281 gcatcgaccc ggtcgatttc gaccgccacg gccatggcgg tgccctccgg cgtgtgcgcg
     5341 aacacgttct cgagcaaagc gtcgacagca gccccgagat cgtcggcgga cacccggacg
     5401 aaccgcgaca ccgtcggcag tcgcagctcc acccggcgtc cctgatcttc ggcaagcacc
     5461 gaccagaacg ccacgcgctc tttcaccacg ctgacgacgt cgcaggccgg tgtcgcttgc
     5521 tctcgcaccc cgcggcgagc ttcccggatc accgagccca cggttcgctc gagagcgtcg
     5581 acgtgttcag acagtctgac tgcctcgggt gagtccggca gcgcctcggc cgccaacttc
     5641 acggcagcaa ccggggtgcg caggcgatgg gacagatcgg caacctgctc gcgttcgtcg
     5701 tcaagcagct cgttgatccg ctgggccaac cggttcatcg ctacgccgac ttcgacgacc
     5761 tcgcgcgggc cgttgacggg ggcacgggca agcagctgcc cggacgacag tcggttggca
     5821 gtcgccgcta gcgcggcgac cgggcgcacg gtccactgcg cgaggcggtc ggcgatcacc
     5881 atcgccaccg cgaggatcgc gatccccacc agcaccagga tcacgatcgc ggagaacaca
     5941 ccttggtcgt agagggccgc agagacgaag gtccggacga cgatgggctt gcctgcgatg
     6001 tcggtgaccg ggtgccagat ctccagcccg cccttgtagc gaacc
//
LOCUS       DATCBJ010000253         2257 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_216506, whole genome shotgun sequence.
ACCESSION   DATCBJ010000253 DATCBJ010000000
VERSION     DATCBJ010000253.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2257)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2257)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2257
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_216506"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            25..330
                     /locus_tag="VII50_05220"
     CDS             25..330
                     /locus_tag="VII50_05220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5050279.1"
                     /translation="MSAMLHLTREAFGIELRRGTFEILVDGATVGSIKRHETVETPLE
                     PGHHTLRLRSGRYSSGDHSFELADDEVVNFRGHGAMVWPRYVISIVKPDLAISLKRE"
     gene            490..672
                     /locus_tag="VII50_05225"
     CDS             490..672
                     /locus_tag="VII50_05225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5050280.1"
                     /translation="MAKEVTCPPCGAIVRGNDDSELLANVRQHALDHGHEVPLGMTVE
                     DFEAQILSEAREVSSA"
     gene            910..1302
                     /locus_tag="VII50_05230"
     CDS             910..1302
                     /locus_tag="VII50_05230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5050281.1"
                     /translation="MSGPFIFIATNRLKPGALEAEKRRVPQLCEFIEATEPRMLAFNE
                     YVDDNGSEVAVVQVHPDAESMEFHMGVVREQAERAYAETLDSTISIQVYGMPSDAVLD
                     TLSRQAGSGLSLTVRRHHLGGFTRPTTA"
BASE COUNT          408 a          694 c          753 g          402 t
ORIGIN      
        1 aggcgatacg aaaggagagc aacggtgtca gcaatgcttc acttgacgcg ggaagccttc
       61 ggcattgagc ttcgccgcgg cacgttcgag atcctcgtcg atggcgcgac ggtcggctcg
      121 atcaagcgcc atgaaacggt cgagacgccg cttgagccgg gacaccacac gctcagactt
      181 cggtcaggcc gatattcgag cggagaccat tcgttcgagt tggccgacga cgaggtcgtg
      241 aacttccgtg gtcacggagc gatggtctgg ccgcggtacg tcatatccat cgtcaagccc
      301 gacttagcga tctcactcaa gcgcgaatag cggtgggcgc gacgcggctc gacgcctcgc
      361 tcggagtcgt ggcgcgccgt cggtggcgcg acgaacgagc agctgcgacg acgcaccgcg
      421 cgtgttgcaa tccgattggc gcggatgtac gctcgcgacg acttcgtccg agaactagcg
      481 gaggtcctaa tggcgaagga agtcacgtgc ccaccgtgcg gagcgatcgt ccgcggcaac
      541 gacgactccg aactactcgc gaacgtgcgc cagcatgcgc tcgatcatgg acatgaggtg
      601 ccgctgggga tgacggtcga ggacttcgag gcgcagatcc tcagcgaggc gcgcgaagtc
      661 tcgtcagcct gacgcgacat ccaaccggga gagcggggcg ccaccgccgc tgcgccccgc
      721 aactcggacc cgttgggcga caccctgcac tgccgcctgg tgcgagcagc cacgccggcg
      781 cggtcgtctt cagcgcgtgc gcggatcgtc ggctctgttg tccgagcccg cgctcgggtt
      841 gtcggagtgt gcgacatccg acacgatgga gtcggaagtt cgcgatgccc aatcgaccaa
      901 ggagatggca tgtcggggcc gttcatcttc attgccacaa acaggctcaa gccgggcgca
      961 ctggaagcgg agaagcggcg cgttccgcag ctgtgcgagt tcatcgaggc gaccgagcct
     1021 aggatgctcg cgttcaacga gtacgtggac gacaatggca gcgaagtggc cgtcgtccaa
     1081 gtccacccgg acgccgagtc gatggagttc cacatgggcg tcgtacgcga gcaagcggag
     1141 cgtgcgtacg cagagaccct cgattccacg atcagtattc aggtgtacgg gatgcccagc
     1201 gacgccgtgt tggacacgtt gagtcggcaa gccgggtcag gcctttctct cacggtgaga
     1261 cgccaccacc tgggtggatt cacgcggccc acgacggcct agctatcgac gcacgaagtc
     1321 gccaccgcag cctgcctgag tgtcctttgc gcgagcagca acagcgctgc gtgcaacgct
     1381 ggaccgacac ggtgcccccg gtccggatca gggcgtggtc cgcgctcgca atagcgcttc
     1441 gccggtgctc cactcctcag cgaaccccgt atggggcgat gcagtgtcga tgatcgcctt
     1501 gacagcgacg aggaaaagtg atatcacttc gataccactt tttccaaaga ggttcccaag
     1561 gaggagccat gaaaattaga tgggctgacg aaaacgcgcg tggtgtgatg aggcttcttc
     1621 ctgcgttcga ataccggcgc ccgaggctct tcgtcccgat acgtgtggtt gccggtgtct
     1681 ggatgcttat cctcacggcg attctgtatg gctacaaccg tggcggttgg tggggagccc
     1741 tgctaatacc ggccgcagca ggtcacttct actgcgccta tcgtttgaat cgcttgccac
     1801 gagcaggcag cgcaaggatg agttcgccgc aggcgaactg acggtggcgc ggatgtctgc
     1861 cgggcatcct gcccggccgg gcatcgcgcg agccgacgcg gcgaagaccg gggcggcgat
     1921 cgcgcttccg tgcacgatca ccggatccac gccgcggccg gtatcgccgc ccccatcagc
     1981 tcgccggagg cttagccgtg tcgctcgccg tcctgttcgc cgcgctgggc tgtgagcgcc
     2041 gagtctcacg gagcgacgga gaacgggacg ttggagtcgc ttcggttggt gagcgtcgag
     2101 gcgtaggagg acttggcttc gctgttcacc tcatgccgct atgggtagcc tccggaccca
     2161 tagccgatgc ctgccaggaa gtgctggcat ttctgcagcg cgttcttgac ctgcggcgca
     2221 ttctggttga cgcccggcat attgcggggg aagccag
//
LOCUS       DATCBJ010000254         2855 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_397870, whole genome shotgun sequence.
ACCESSION   DATCBJ010000254 DATCBJ010000000
VERSION     DATCBJ010000254.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2855)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2855)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2855
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_397870"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..320)
                     /gene="ribH"
                     /locus_tag="VII50_05235"
     CDS             complement(<1..320)
                     /gene="ribH"
                     /locus_tag="VII50_05235"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HEY5050282.1"
                     /translation="MTGTGRPDPGAVAATGLSLAIVAARWNTEVVDALLERALEAARA
                     CGVPKPTVVRVAGALELAVIGQQLAVEHDAVVCLGAVIRGGTPHFDYVCDAVTAGLTR
                     IALDA"
     gene            complement(317..1588)
                     /locus_tag="VII50_05240"
     CDS             complement(317..1588)
                     /locus_tag="VII50_05240"
                     /EC_number="3.5.4.25"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019513470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA];
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HEY5050283.1"
                     /translation="MTFDTIERAIEEIAAGRAVVVVDDEDRENEGDLIAAASKVTPEL
                     VAFMVRHTSGVLCVPMKSATLDRLGLPPMTARNEDAKQTAYTVSVDARRGVTTGISAA
                     DRAHTIRVLADSATEPYELTRPGHVFPLRAVEGGVLRRAGHTEAAVDLAVLAGLTPAG
                     VISEIVNDDGSMARLSRLVEFAAEHDLAIVSIADLVAYRRRHETTVERIAEARLPTFH
                     GEWRAVGYRSLLDGAEMIALVMGEVGGSDDGQDVLVRAHSECLTGDVFGSLRCDCGPQ
                     LDAAMSAIAAEGRGIVLYIRGHEGRGIGLMRKLHAYELQDSGADTVDANLELGLPADA
                     RDYGTGAQILVDLGVRSMRLLSNNPAKRVGLEGWGLRVVGRVPLPTHATPDNMRYLLT
                     KRDRMGHDLLLPGHPDLIPVPPADDQPGALS"
     gene            complement(1585..2292)
                     /locus_tag="VII50_05245"
     CDS             complement(1585..2292)
                     /locus_tag="VII50_05245"
                     /inference="COORDINATES: protein motif:HMM:NF016837.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0034257 - nicotinamide riboside
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide transporter family
                     protein"
                     /protein_id="HEY5050284.1"
                     /translation="MSAINWLYTAQWHVGGHAIYYREIFGNLFGLASAVGGMRRRVWA
                     WPVGIVGNVMLFTVFLGSWASHSHQAPLIGQAGRQVFFVITSIYGWNRWGHARRTSSD
                     APAVTPRWATSRERKLLLGAAAVGVAVCYVAFHLIGEGFHALWWYYLADSWIFVGSMV
                     ATYAMARGWTDFWLCWIAVDVVGVPELLHFHYYPSAALYAIYAGFVIWGFFVWRQLQR
                     TAPTLSELSPVPQEATG"
     gene            complement(2289..>2855)
                     /locus_tag="VII50_05250"
     CDS             complement(2289..>2855)
                     /locus_tag="VII50_05250"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020658132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HEY5050285.1"
                     /translation="AVGNERAVRVTISGPLVSSDVAIGDSIAVSGVCLTVVDAASGRF
                     SSDVMRETLTRSTLNGAHAGTPVNLERAVTLQTRLGGHLVQGHVDGTGTVVRREHTPE
                     WDIVEIAIPPALARYVVEKGSIAVDGVSLTIAAVGAESFTVSLIPTTLTRTTLGEKAV
                     GGAVNLEVDVMAKYAERLLGFATAQVGP"
BASE COUNT          522 a         1010 c          887 g          436 t
ORIGIN      
        1 gcgtccagcg caatacgggt gaggccggcg gtcactgcgt cgcagacata gtcgaagtgc
       61 ggcgtgccgc cccggatcac cgcaccaaga cagacgaccg catcgtgctc gaccgcaagc
      121 tgttgcccga tcaccgccaa ctccagtgcg ccagcaaccc ggacgaccgt gggcttgggc
      181 acgccgcacg cgcgcgccgc ctcaagggcc cgttcgagca aggcgtcgac gacctcggta
      241 ttccagcggg cggcgacgat ggccaggctc agtcccgtcg cagcgacggc accgggatca
      301 gggcgtccgg taccggtcat gaaagcgctc ctggctggtc gtcggctggc ggtaccggaa
      361 tcaagtcagg gtggcccggc agcaacaggt catgtcccat ccggtcgcgc ttggtcagca
      421 ggtaccgcat gttgtcaggg gtggcgtgcg tcggcaacgg aacccggccg acgacccgca
      481 ggccccaacc ctcaaggccc acccgcttcg ccggattgtt cgacagcaag cgcatcgacc
      541 gcacaccgag gtcgacgagt atctgggcgc cggtgccgta gtcacgtgcg tcagccggaa
      601 ggccgagctc gaggttcgcg tcgaccgtgt cagcgccgct gtcctgcaac tcgtacgcat
      661 gcagcttgcg catcagcccg atgccgcgcc cttcgtgccc gcggatgtac aacacgatgc
      721 ctctgccctc cgccgcgatc gccgacatgg cggcgtccaa ctggggacca cagtcgcacc
      781 gcaacgagcc gaacacgtca ccggtcaggc actcggaatg cgcgcgcacc agcacgtcct
      841 ggccgtcgtc ggatccgccg acctcgccca tgaccagcgc gatcatctcg gcgccgtcga
      901 gcaggcttcg atacccgaca gcccgccatt ccccatggaa tgtgggcagt cgtgcctcgg
      961 cgatgcgctc gaccgtcgtc tcgtgccgcc gccggtatgc gacaaggtcg gcgatggaga
     1021 cgatcgcaag atcgtgctcg gccgcgaact ccactagccg cgacagccga gccatcgaac
     1081 cgtcgtcgtt gacaatctca ctgatgacgc cggccggtgt caggcctgcg agcaccgcga
     1141 ggtcgaccgc cgcttcggtg tgcccggccc gtcgcagcac cccgccctcg accgcgcgca
     1201 aggggaacac atgccccgga cgggtcagtt cgtacggctc ggtagccgaa tcggcgagga
     1261 cccgaatcgt atgtgcacga tcagctgcgg aaattcccgt cgtcactccc cgccgcgcat
     1321 cgaccgagac cgtgtacgcc gtctgctttg catcttcgtt gcgtgctgtc atcggcggca
     1381 ggccaagccg gtcgagcgtt gcgctcttca tcggcacgca cagcacgcca cttgtatggc
     1441 gcaccatgaa cgcaaccagc tcaggtgtca ccttggacgc cgcggcgatg aggtcgcctt
     1501 cgttctcgcg gtcttcgtcg tcgaccacga caacagcgcg accggcggca atttcttcga
     1561 tcgctctctc gatggtgtcg aacgtcatcc ggtcgcctcc tgcggcactg gtgagagttc
     1621 actaagggtg ggcgccgttc gctgcagttg ccgccatacg aagaatcccc agatcacgaa
     1681 ccccgcgtaa atcgcgtaca acgcggccga cgggtagtaa tggaagtgca gcaactccgg
     1741 cacgccgaca acgtccacgg cgatccagca caaccagaag tcagtccagc cacgcgccat
     1801 cgcgtacgtc gcgaccatcg agccgacgaa gatccaggag tcggcgaggt aataccacca
     1861 cagcgcgtgg aagccctcgc cgatcaggtg gaacgccacg tagcacaccg caaccccgac
     1921 cgcggcagcg cctaacaaga gcttgcgctc tcggctcgtc gcccagcgcg gcgtcacggc
     1981 tggagcatcg gatgaggtcc gccgcgcatg cccccagcga ttccagccgt aaatgctggt
     2041 gatcacgaag aacacctgac gcccggcctg cccgatcagc ggtgcttggt gggagtgcga
     2101 ggcccagcta ccgaggaaga cggtgaagag catcacgttg cccacgatgc ccaccggcca
     2161 tgcccagacc cgacggcgca tgccaccgac tgcggaggcc agcccgaaga ggttgccgaa
     2221 gatctcgcgg tagtagatcg cgtgaccgcc gacatgccac tgcgcggtgt acagccagtt
     2281 gatcgcgctc atgggccaac ctgcgcagtc gcgaacccga ggagccgctc ggcgtacttg
     2341 gccatcacgt ccacctcgag attgaccgcg ccacccacgg ccttctcgcc cagcgtcgtg
     2401 cgcgtcaacg tcgtcgggat caggctgacc gtgaaggatt cggccccgac ggccgcgatc
     2461 gtcaacgaca ctccgtcgac cgcgattgag cccttctcaa cgacgtaccg cgccagcgcc
     2521 ggcggaatgg cgatctccac gatgtcccac tctggcgtgt gctcgcggcg aaccacggtg
     2581 ccggtgccgt cgacgtgacc ctgcacgaga tgaccgccca atctggtttg aagggtgacc
     2641 gcacgctcga gattcaccgg cgtgcccgcg tgtgcaccgt tcagcgttga gcgagtcagg
     2701 gtttcccgca tcacatcgga gctgaagcgt cccgacgccg cgtcgacgac ggtcaagcag
     2761 acaccggata cggcaatcga gtcaccgatg gcaacgtcgg agctgaccag cgggcccgag
     2821 atggtcacgc gcaccgcccg ctcgttgccg accgc
//
LOCUS       DATCBJ010000255         2644 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_4204, whole genome shotgun sequence.
ACCESSION   DATCBJ010000255 DATCBJ010000000
VERSION     DATCBJ010000255.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2644)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2644)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2644
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4204"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..676)
                     /locus_tag="VII50_05255"
     CDS             complement(<1..676)
                     /locus_tag="VII50_05255"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY5050286.1"
                     /translation="MPVDPRVAPLIGRVDELAALCDVVGVGATAGGAVVLGGDAGAGK
                     SRLVAELSERARSQGWRVLVGHCLDVGDGSPPYLPFSEALGRLAADSPPEAESLLAAS
                     PAIARLLPAQRLLTESDHAMEPTGRTALYDALRGALTQLSADSPLLLIIEDVHWADQS
                     TRDLLRFLFARQFTSPTAILATYRTDDLHRSHPLRAALAEWTRLSTVSRLQVGPLTDA
                     ESRLLIR"
     gene            complement(717..941)
                     /locus_tag="VII50_05260"
     CDS             complement(717..941)
                     /locus_tag="VII50_05260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5050287.1"
                     /translation="MLEHFTDAELAYRRGQLQRSGRPGRQLAGPSLLQRFRDARRSRR
                     NPVRGALRYELDGPVTRRIEDYWISAGTHH"
     gene            complement(1427..2353)
                     /locus_tag="VII50_05265"
     CDS             complement(1427..2353)
                     /locus_tag="VII50_05265"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HEY5050288.1"
                     /translation="MAEVQRPAGPQIDESQGLASVGAQLWLLTGALMLLVDESDGIVA
                     ANPAMCVAVGLAEVDLIGRDAVEVVVPREVADFRHELRAASRSGVPSSCEHELRTRTD
                     HDRRVVAWSMSRTSRSPVIVGCIGVDVTAARNDSDVLRQRALTDELTGLPNRSGLRDH
                     LARMAGSGASVVFCDLNGFKAVNDTHGHDIGDAVLVQVARRLKRTVRGEDFVARIGGD
                     EFVIVAPPDPNSDFDMLARRILRATDQPMILPGPVVATVGMSIGMAVLEPGTDPATAL
                     TLADHDMYRMKSRRTTEATSAAGVGSNLDVPD"
BASE COUNT          471 a          878 c          886 g          409 t
ORIGIN      
        1 cgcggatcaa cagccgcgac tccgcgtcgg tcagcgggcc gacttgcagg cgcgagaccg
       61 tcgacaggcg cgtccactca gcgagcgctg cgcgcagcgg gtggctacgg tgcagatcgt
      121 cggtgcggta cgtcgcgagg atcgcggttg gcgaggtgaa ctgccgggcg aacaggaagc
      181 ggagcaagtc gcgtgtcgac tgatcggccc agtgaacgtc ttcgatgatg agcagcagcg
      241 gcgagtcggc gctgagctgg gtcagcgcgc cgcgcagtgc gtcgtacagc gccgtacgcc
      301 ccgtcggctc catcgcatgg tctgactcgg tgagcagccg ctgcgcgggg agcagcctgg
      361 cgatcgccgg gctcgcggcg agcagtgatt ccgcctcggg cgggctgtcg gcggcgagcc
      421 gacccagcgc ctcgctgaac ggaaggtacg gcggggaacc gtctcccaca tccaggcagt
      481 gtccgacgag cacccgccag ccctgagatc gagcccgctc gctcaactcg gcgacaaggc
      541 ggctcttgcc cgcgccggcg tcgccgccga gcacgaccgc gccaccggcg gtcgcgccga
      601 cgccgacgac gtcgcacaac gctgcgagct catcgactct cccgataaga ggcgcgacac
      661 gcgggtcaac aggcacgcct gccatcatga cggacgcgac caccgagttt ctccggtcag
      721 tggtgcgttc cggcgctgat ccagtagtcc tcgatccggc gggtcacggg tccgtccaac
      781 tcgtagcgca acgcgccgcg aaccgggttg cgccggctgc gacgcgcgtc gcggaaccgc
      841 tggagcaggc taggtccggc gagttggcgg cctggacgac cactgcgctg gagctgcccg
      901 cgacgatagg cgagctctgc atcggtgaag tgctcaagca tgggaatcgg tctcctctgt
      961 ggcgactgcg ctttgctggc gagatcaacg gtgcgcgtat gaggtagtgc gccacatccg
     1021 aaggaatccc tatcttcgga acccggtttg actcgggttg gcttagcccg agccggatgt
     1081 cttaggcggg cttagacagc ctcggtccag agtcctgcaa ggggttgcgg gcaggccgtc
     1141 ccatcggagt cggcggacga gccaggcgcc ctacgaccgc tccgaaagcc ctgcaccgcc
     1201 gtcccgcccc tcgcagcggg ctccttgatt gcgccgccgg taatgcctga ggcgataaat
     1261 cgctgggcgt acatgagtag gacggccgcc ggcgcacacg ccacgaccgc tgtcgccatg
     1321 acgggagctc gtgagtcaat gatgatccgt tgattccctg ttgcgaaagg ctggtcgcca
     1381 agctagccgg tcgcaagcgg ccatcctcgc cgagacgacg cgttgctcag tcgggtacgt
     1441 cgaggtttga gccaacgcct gcggcgctgg tggcttcagt ggtgcgccgg gatttcattc
     1501 ggtacatgtc gtggtcggcg agggtgagcg ccgtggcggg atcggtgccc ggttcgagaa
     1561 cggccattcc gatcgacatc cccacagtgg caacaaccgg gccgggcagg atcatcggct
     1621 ggtcggttgc ccgcaggatc cggcgggcga gcatgtcgaa gtcggagttg gggtcgggtg
     1681 gggcgacgat gacgaactca tcaccgccaa tccttgcgac gaagtcttca ccgcgcaccg
     1741 tgcgctttag acggcgggcg acctgcacca gcaccgcgtc cccgatgtca tggccgtgag
     1801 tgtcgttgac ggccttgaaa ccgttgaggt cgcaaaacac caccgaagcg ccgctacccg
     1861 ccatccgtgc cagatggtcc cgaagacccg accggttcgg caatccggtc aactcatcgg
     1921 tcaaagcgcg ttggcgcagc acgtcggagt cgttgcgcgc ggcggtgacg tcgacgccga
     1981 tgcaaccgac gataaccggg gagcgcgacg tccgcgacat ggaccatgcg acaacacggc
     2041 ggtcgtggtc ggtgcgggta cgaagctcat gctcgcagct ggacgggacc ccggagcgcg
     2101 acgcggcgcg caactcgtgc cggaagtccg caacctcacg cggcaccacc acctcgaccg
     2161 cgtcgcggcc aatcaggtcg acttctgcca gcccaaccgc gacgcacatc gcaggattcg
     2221 ccgcgacgat cccatcggac tcatccacca gcagcatcag cgcaccagtc agtagccaca
     2281 attgcgcgcc aacactcgcc agcccctgcg attcgtcgat ttgcgggccg gccggccgct
     2341 gtacctccgc cacgctccac ctcagattcc gctgccatcc catcggcagt cggccggcac
     2401 cggcgcagga ctttcaatcg gcagatagca aaacaccatg gagaacgcga gccagctcac
     2461 gatttaactg aaggaaacgc acagggcgac cagtaggggc ggacgagtcg gcctataggc
     2521 cgggccgatt caagccgctg aagccccaga gttgccgcag gtcaacgcta cctttcgccc
     2581 gtcggaagcc atcgatgtca caccgatgtc acgactggga agcgatggtg gatccaatgg
     2641 cgta
//
LOCUS       DATCBJ010000256         2445 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_128807, whole genome shotgun sequence.
ACCESSION   DATCBJ010000256 DATCBJ010000000
VERSION     DATCBJ010000256.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2445)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2445)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2445
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_128807"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1784)
                     /gene="topA"
                     /locus_tag="VII50_05270"
     CDS             complement(<1..1784)
                     /gene="topA"
                     /locus_tag="VII50_05270"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HEY5050289.1"
                     /translation="MARASTPAASDDDTRRLVIVESPAKAKTIAGYLGGNYIVESSIG
                     HIRDLPTSAADIPAKYKKETWSRLGVDVDHGFEPLYVVNADKRQQVAKLKAALAGVDE
                     LYLATDEDREGEAIAWHLLEVLNPKVPVHRMVFHEITRDAIREAAQHPRELNQALVDA
                     QETRRILDRLYGYEVSPVLWKKVMPRLSAGRVQSVATRMVVDRERDRIAFRSASYWDV
                     NAVFDTTPTFEATLAAVDGQRVAQGRDFDSVGVLKTKNLVHLDEAAATVLATGLSNTE
                     FVVRSVESKPYRRSPAAPFMTSTLQQEASRKLRFSSQTTMRIAQRLYENGHITYMRTD
                     STTLSETALNAARNQVRELYGNEFLPDVPRRYEKKVKNAQEAHEAIRPSGDRFATPGE
                     LAGQVTGDEARLYELIWQRTVASQMTDATGQTVTMRLGGTTRPDEANTSHDAEFSASG
                     TVIVHQGFLKVYVESTDDDTSDDTSERRLPNLSEGDTARATSLEPVGHTTNPPARYTE
                     ASLVRALEERGIGRPSTFASIMGTILDRGYVFKKGSALVPTFLAFAVVQLLERHFGDL
                     VDYDFTAEMEEDLDRIADGETARTAWLT"
     gene            complement(2017..>2445)
                     /locus_tag="VII50_05275"
     CDS             complement(2017..>2445)
                     /locus_tag="VII50_05275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_function: GO:0009678 - diphosphate hydrolysis-driven
                     proton transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proton-translocating pyrophosphatase"
                     /protein_id="HEY5050290.1"
                     /translation="GVGALAGYLVGAIAAGALMAVFLANSGGAWDNAKKLVEEGNHGG
                     KGSPAHEATIIGDTVGDPFKDTAGPAINPLLKVMNLVALLIAPLIVRYSVGTHHNAPL
                     RIIIAVVAFAIIAASVVISKRRSVAVGAGEDEDTPSAVAV"
BASE COUNT          366 a          788 c          834 g          457 t
ORIGIN      
        1 tgcgtcagcc acgccgtgcg cgctgtctcg ccgtcggcga tgcggtcgag gtcttcttcc
       61 atctcggcgg tgaagtcata gtcgacaagg tcgccgaagt gccgctccag caactgcacg
      121 accgcgaacg cgaggaaagt cggaaccagc gcgctgccct tcttgaacac atacccgcga
      181 tcaaggatgg tgcccatgat gctggcgaac gtgctcgggc ggccgatgcc acgttcttcc
      241 aacgccctga cgaggctcgc ttcggtgtag cgggccggcg gattcgtcgt gtgcccgacg
      301 ggttcgaggg acgttgcccg ggcggtgtca ccctctgaca agttcggcag gcggcgctcg
      361 ctcgtgtcat cggaggtgtc gtcgtccgtg ctctcgacgt agaccttcaa gaagccctga
      421 tgcacgatca ccgtgccgga cgccgagaac tccgcatcgt gggacgtgtt cgcctcgtcg
      481 ggccgggtcg tgccacccag gcgcatggtc accgtctggc cggtggcgtc cgtcatctgc
      541 gacgcgaccg tgcgttgcca gatcagctca tacaggcggg cctcgtcgcc ggtgacctgc
      601 ccggcgagct cgccaggggt ggcgaacctg tcgccactcg gtcggatcgc ctcgtgcgcc
      661 tcctgcgcgt tcttgacctt cttctcgtac ctgcgcggga cgtcgggcag gaactcattt
      721 ccgtacagct cgcgcacctg attgcgggcg gcgttcagtg cggtctccga cagggtcgtg
      781 gagtcggtac gcatgtaggt gatgtggccg ttctcgtata gccgctgggc gatccgcatc
      841 gtggtctggc tgctgaaccg cagcttgcga ctcgcctctt gttgaagcgt gctcgtcatg
      901 aacggagccg ccgggctacg ccggtacggc ttgctctcga cgctgcgcac gacgaactcg
      961 gtattgctca agcccgttgc cagaacggtt gctgcggcct cgtcgaggtg caccaggttc
     1021 ttggtcttca ggacgccgac ggagtcgaag tcacggccct gcgcgacgcg ttggccgtcg
     1081 accgcggcga gggtagcctc gaaggtcggg gtcgtgtcga agaccgcgtt gacgtcccaa
     1141 taggaagcgc tgcgaaacgc gatgcgatcg cgctcgcggt cgacgaccat gcgggtggcg
     1201 acgctttgca cccgaccggc ggacagccga ggcatgacct tcttccacaa gaccggactg
     1261 acttcgtagc cgtagagccg gtcgaggatc cgccgggtct cctgcgcgtc gacgagcgcc
     1321 tggttcagct ctcggggatg ctgcgcggct tcgcgaatcg cgtcacgggt gatctcgtgg
     1381 aagaccatcc ggtggacggg caccttcggg ttgagcacct cgagtaagtg ccacgcaatg
     1441 gcctcgcctt cgcggtcctc gtcagtcgct aagtagagct cgtcgacgcc ggccagcgct
     1501 gccttcagct tggcgacctg ctggcgcttg tcagcgttga ccacgtagag cggctcgaag
     1561 ccgtggtcga cgtcgacgcc gaggcgtgac catgtctcct tcttgtactt ggcggggatg
     1621 tcagcagccg aggtcggcag gtcgcggatg tgcccgatgc tcgactcgac gatgtagttg
     1681 ccgcccagat acccggcgat cgtcttcgcc ttggctgggg actccacaat gaccagacgg
     1741 cgggtgtcgt cgtcggatgc agcgggagtg ctggcgcgcg ccactacggg tgctcctcat
     1801 cggtggtcaa ggggtctatc ggacgccagc agtggttgat tcagggctgg ttcaggggtc
     1861 aaacaggtgc cggcgtcccg agccacggta accgctcgcc accaccgacg tggccactga
     1921 ccccgcgacg gagcaattcg ccgcgcgacg gtgggtccgt cggcgttcga cctgtgactc
     1981 ttttgccgcc ctcgccgggc ggccgtcaga gcaccgctag accgcgacgg cggacggcgt
     2041 gtcttcgtct tcgcccgcac ctacggcgac gctgcggcgc ttggagatga ccaccgacgc
     2101 ggcgatgatc gcgaacgcca ccacggcgat gatgatgcgc aacggcgcgt tgtggtgggt
     2161 gcccacggag taccggacga tcagcggcgc aatcagcagg gcgaccaggt tcatcacctt
     2221 gagcagcggg ttgatggccg ggccagcggt gtccttgaag gggtcgccga cggtgtcgcc
     2281 gatgatggtc gcctcgtggg ctggcgagcc cttgccgccg tgattgcctt cctcgaccag
     2341 cttcttggcg ttgtcccacg cgccaccgga gttcgcgagg aacaccgcca tcagcgcgcc
     2401 agcggcaatg gcgccgacga gatagccggc gagtgcgccg actcc
//
LOCUS       DATCBJ010000257         2243 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_191139, whole genome shotgun sequence.
ACCESSION   DATCBJ010000257 DATCBJ010000000
VERSION     DATCBJ010000257.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2243)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2243)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2243
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_191139"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            320..1102
                     /locus_tag="VII50_05280"
     CDS             320..1102
                     /locus_tag="VII50_05280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016460186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HEY5050291.1"
                     /translation="MGGGVPAMDVRGFLYGAYERRIAAELTPDRVPRHVGVILDGNRR
                     WAKALGVGVASGHRKGAAKIDEFLGWCSELHIEVVTLWLLSTDNLNRPEEELAPLFRI
                     IEDAVSELAVGRRWLINPVGALDLLPDATARSLKEAQDATADLTGLIVNVAVGYGGRR
                     EIADAVRALLHEHAGRGTSIEDLANLLDIDHIAEHLYTKGQPDPDLVIRTSGEQRLSG
                     FLLWQSAHSELFFCEAYWPDFRRVDFLRAVRAYAERERRFGS"
     gene            1972..>2243
                     /locus_tag="VII50_05285"
     CDS             1972..>2243
                     /locus_tag="VII50_05285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5050292.1"
                     /translation="MSRIPAPGAIVGTTTISAVLALCLLGSVGAQAPAAADESTTAPS
                     TIGASLIAAENTATVVGLTTTSTLSGATTNAAVMTNAAVMTNAAVMT"
BASE COUNT          413 a          706 c          696 g          428 t
ORIGIN      
        1 gacacgctgg tcgacgccgg cgtggcgcgc agccgtagcg atgcgctcgc gtggtgcgtg
       61 cgcctggtcg ggaagaacac cgacgaatgg ctcggccagt tgcgcgatgc gctccgtacg
      121 gtcgagcagg tgcgggacgc cggcccctcg ccgtcctagt acgagggttg cccgttgtcg
      181 ccatagagcg cgcccacctt cgcgagaact cttccccatg cggggtaagg ttcggccaag
      241 aagtagtggg cgcgccgtcg cgcccagacc cgcagcagtc gatcgccgca cctcggagcg
      301 gctcgcgcgg gcgtgcgcga tgggaggtgg cgtccccgcg atggacgtac gcggcttcct
      361 ctacggggcg tacgagcgcc ggatcgccgc cgagctgacg cccgaccgcg tcccgcgcca
      421 cgttggggtc atcctcgacg ggaaccgtcg ctgggcgaag gcgctagggg tcggagtcgc
      481 ctcgggccat cgcaagggcg ccgcgaagat cgacgagttc ctgggctggt gctccgaact
      541 gcacatcgag gtggtcaccc tctggctgct ctccaccgac aacctgaacc ggcccgagga
      601 agagctcgca ccgctcttcc ggatcatcga ggacgcggtg agtgagctcg cggtcggccg
      661 ccgctggctc atcaacccgg tcggagcgct cgatctgctc ccagacgcca ccgcgaggtc
      721 cttgaaagag gcccaggacg cgaccgccga cctgaccggg ctgatcgtga acgtcgccgt
      781 cgggtacggc gggcgcaggg agatcgccga cgccgtccgg gcgctcctgc acgagcacgc
      841 cggccggggc accagcatcg aggatttggc caatttgctc gatattgacc atattgccga
      901 gcacctctac acgaagggcc agcccgaccc tgacctggtg atcaggacct cgggggagca
      961 gcgattgtca gggttcctgc tatggcagtc cgcccactcc gaactgttct tctgtgaggc
     1021 gtactggccg gacttccggc gggtcgactt cctgcgggcg gttcgcgcgt acgccgaacg
     1081 cgagcgtcga ttcggctctt agcacgccga gttaccgcag gacagttgcc aacgatgtgc
     1141 gcaaagtgcc gaccgattgg tgggcggttt gactgccgac ctgtccggcg ctgggtggcc
     1201 gctcggcgaa aaatccgcat gtcgaattgc gcgacgaatc gatcgccgat ccgctgctca
     1261 ttcatgcgac agcatgtctt gccgatcaat cgagcatctc tctcgacacc gagacacatg
     1321 tctcgacatc gagataacag aatgaactgt atggctgtct gcctctgcgg gttcgcaacc
     1381 gtgcacaaca tgagatgcat tgagagacgc gttggatggt ctactacgcg aaaggccgaa
     1441 ggtgaccgtc gacgttgtgc aaaccaacgc agctacggac ggtgcaactg cgggtcgccc
     1501 gcaacggcga gctgaatgcc gagaacacct tagtttgaag caatatctcc atgtgtcgac
     1561 aaagtgcctg cggtcgcact ctcacgaaga gtcaccaacc gaacgcgctc ggtacccccg
     1621 tggaatgctc gaatacatag agtttttgca atgccagtga taaatcttcg gctcagtagt
     1681 cacaacaatc acacatacag cctgttgtga catgcttcac cgctcggggc tttgcgtcca
     1741 tgcgttcatt cgtgcatgct ggcaggacac cccatgcatt tcggccggta cctgcggtcc
     1801 gttgcatgcc gtatcgctct cagacgcgca ttagcgctgc ggtacatggt tgaccatctt
     1861 ctttggtttg acctgggtcg ttcagaagca ctgcgtcatt tcgaagcact gcgtcttttc
     1921 acaagcactg cgtcattgct cgcatgcttg ctcccgaaag gaactcattc aatgagccgg
     1981 atcccggccc ccggcgcgat cgtcggcacc acgacgatct ctgccgttct tgcgttgtgt
     2041 ttgctcggta gtgtcggtgc acaagcaccg gctgccgccg acgaatcaac gactgcacca
     2101 agcacgatcg gcgcgagtct cattgctgcc gagaacacag caactgttgt aggtctcacg
     2161 acgacgtcaa ctctgtctgg tgccacgacg aacgctgctg tgatgacgaa cgctgctgtg
     2221 atgacgaacg ctgctgtgat gac
//
LOCUS       DATCBJ010000258         3505 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_373382, whole genome shotgun sequence.
ACCESSION   DATCBJ010000258 DATCBJ010000000
VERSION     DATCBJ010000258.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 3505)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3505)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3505
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_373382"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..480)
                     /locus_tag="VII50_05290"
     CDS             complement(<1..480)
                     /locus_tag="VII50_05290"
                     /inference="COORDINATES: protein motif:HMM:TIGR03941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine deaminase-associated protein"
                     /protein_id="HEY5050293.1"
                     /translation="MSSDRRPGAREASSGSEDDAGRDFAVVCYREEGRWELGLLPERA
                     SSSLESLISVLRQQPGEVTVGLVDIADDFFIAVRLSGEGVRLLLSDVTAADEWALARE
                     ALEWLGVPEPADEDFDDVVPAGDLALFSDLGLPEIELGLMLSDIDAYADEMLFSIARR
                     "
     gene            577..1224
                     /gene="upp"
                     /locus_tag="VII50_05295"
     CDS             577..1224
                     /gene="upp"
                     /locus_tag="VII50_05295"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007589358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HEY5050294.1"
                     /translation="MRTLVADHPLIAHKLTTLRDQRTDSPTFRRLVDELVTLLAYEAT
                     RDVRTNVVDVVTPVAHARGTRLARPRPLVVPVLRAGLGMLDGMCRLLPTAEVGFLGLV
                     RDEDTLVATTYASRLPDSLVGRQVFALDPMLATGGTLGAVIKLLLERGADQVTAICLL
                     AAPEGLAFLEEQFGQSTVPVAVVTAAIDERLNERGYIVPGLGDAGDRLYGTVGAG"
     gene            complement(1273..2034)
                     /locus_tag="VII50_05300"
     CDS             complement(1273..2034)
                     /locus_tag="VII50_05300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5050295.1"
                     /translation="MPPGTKLTVVHGTVTVTKAGTVIDSEDIKGQLIIQASNVTVKRS
                     LIEGAKGQDASVDIQFGTGILLEDDEVADTYKSLGNDDMRVRNATLLRLNIHGGVDGM
                     KLFSNSTVSASWIHGLTYFAHDPAQSNGHTHNDTIQILGGTNFHITGNWLDSGTGDNS
                     AIQVTQDIGKISALFITGNWADGGSCSFNFSGHSPSGQAPLYGIAVVNNRFGHTSLRY
                     KGNCAIVDDLTTHLVAATGNVWDTSGTPVTIYSAN"
     gene            2021..2476
                     /locus_tag="VII50_05305"
     CDS             2021..2476
                     /locus_tag="VII50_05305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5050296.1"
                     /translation="MPGGTPVFVGPGLPGGSGAVGDGVGLGVGFGVGVGVGFGVGFDL
                     VVGLPCEREADDEEPVADNADVDEGPVEAPGTTEPSVLELDDDVPGSEGDAADELPGG
                     PGCAEPVLVAGSRSTTAATMSPPSSTSTSDTTTMRSRTEPGNGMTVRSP"
     gene            complement(2463..>3505)
                     /locus_tag="VII50_05310"
     CDS             complement(2463..>3505)
                     /locus_tag="VII50_05310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NAD(P)(+) transhydrogenase (Re/Si-specific)
                     subunit beta"
                     /protein_id="HEY5050297.1"
                     /translation="AVAFTAIVGSVSFTGSLITFAKLQELMTGRPILVPGAQVVTPLV
                     GLGAVGCGIAAVATASHASGSVTFLVICTVLALGLGVLFVLPVGGADVPVVISLLNAF
                     TGLTVAASGYVLDNTLLIVAGTLVGASGTMLTLLMARAMGRSITGVLFGAFSGGSTAG
                     STAEVVSTDRAVKSAGADDIAILLAYARKVAIVPGYGLAVAQAQHTCRELVDLLESRG
                     VEVFYAIHPVAGRMPGHMNVLLAEANVPYEQLKEMDDANPEFSSTDVAVVVGANDVVN
                     PAARDSAGSPIYGMPILNVDEATHVVFMKRSMRPGFAGIDNALLYHPKTTLFFGDAKD
                     SLSKLVAAVKAI"
BASE COUNT          604 a         1133 c         1177 g          591 t
ORIGIN      
        1 cctgcgggcg atcgagaaca gcatctcatc ggcatatgcg tcgatgtcgg acaacatcaa
       61 acccagctcg atctcgggca acccgaggtc ggagaacagc gctagatccc cggccgggac
      121 gacgtcgtcg aagtcctcgt cggccggctc agggacgccg agccactcca gcgcctcgcg
      181 cgccaaggcc cattcgtcgg ccgcagtcac gtcggagagc agcagccgaa cgccctcacc
      241 gctcagccgc acggcgatga agaagtcgtc ggcgatgtcg acgaggccga cggtcacctc
      301 accaggctgc tggcgcaaga ccgagatcaa ggattcaagg ctggacgacg cgcgctcggg
      361 gagcagtccc agctcccagc gcccctcctc gcgataacac accacggcga agtcgcggcc
      421 ggcatcgtcc tccgaccccg agctcgcttc ccgtgctcct ggcctgcgat cgctcgacat
      481 gcctgcatcg tggcacgcgc tccccgggcc agccaatggg agggcgtcga gaatccggat
      541 gtacggttcc gccgctgctc cctataccgt tcgggcatgc gaactctggt ggcggaccat
      601 cccctgatcg cccacaagct gaccaccctg cgcgaccaac gcaccgactc ccctacgttt
      661 cgccggctgg tcgacgagtt ggtgaccctg ctcgcttacg aagcgacccg cgacgtgcgg
      721 acgaacgtgg tcgacgtcgt cacacccgtc gcgcacgccc gcggcacccg gctcgcgcga
      781 ccccggcccc tcgtcgtccc ggtattgcgc gctgggctcg gcatgctcga cgggatgtgc
      841 cggctgctgc ccaccgccga ggtcgggttt ctcgggctgg tgcgcgacga agacacgctg
      901 gtggcgacga cgtacgcctc gcggctgccc gactcgctgg tcgggcggca ggtgttcgcg
      961 ctcgacccga tgctcgccac cggcgggacc ctcggcgcgg tcatcaagct gctgctcgag
     1021 cgcggcgccg accaggtgac cgctatctgc ctgctcgcgg cgccggaagg gctggcgttc
     1081 ctcgaggagc agttcgggca atcgacggtg ccagtcgcgg tcgtgacagc agcgatcgac
     1141 gaacggctca acgagcgcgg ttacatcgtg cccggccttg gcgacgctgg cgaccggctg
     1201 tacggcacgg tcggcgcggg ctagtttgcc ggaaagctgc ggagcagcgg ctagctcgtc
     1261 ggagagctgc ggctagtttg ctgagtagat cgtgaccggc gtcccgctcg tgtcccagac
     1321 gttgccggtc gcggcaacga gatgggtcgt caggtcgtcg acaattgcgc agttgccctt
     1381 gtagcgaagg gacgtgtgac cgaaccggtt gttaaccacc gcgatgccgt agagcggcgc
     1441 ctgcccactg ggcgaatggc cggagaagtt gaacgagcag ctaccgccat cggcccagtt
     1501 gccggtgatg aacaaggcac tgatcttgcc gatgtcctgc gtcacctgaa tcgctgagtt
     1561 gtcacccgtc ccggagtcga gccagttgcc ggtgatgtgg aagttggtgc cgccgagaat
     1621 ctggatcgtg tcgttgtggg tgtgtccatt ggactgcgca gggtcgtgtg cgaaataggt
     1681 cagcccgtga atccagcttg cactcaccgt tgagttgctg aagagcttca tgccgtcgac
     1741 accgccgtga atgttcagcc gcaagagcgt cgcattccgc acccgcatgt cgtcgtttcc
     1801 gagcgactta tacgtgtccg caacctcgtc gtcctcgagc agaattccag taccgaactg
     1861 gatatcaacg ctcgcatcct ggcccttcgc gccctcgatc aggcttcgct tcaccgtgac
     1921 attgctcgcc tggatgatca gctgaccctt gatgtcttcg ctgtcgatca ccgtccccgc
     1981 tttcgtcacg gtgacggtgc cgtggaccac ggtgagcttc gtgcccggcg gcaccccggt
     2041 attcgtcggc ccgggcttgc cgggcggttc gggcgcggtc ggcgacggcg tgggtttggg
     2101 cgtcggtttc ggtgtcggag tgggcgtcgg cttcggcgtc ggtttcgatt tggtggtcgg
     2161 cttgccttgc gagcgtgagg cggacgacga ggagccagtc gccgacaacg cagacgtcga
     2221 cgagggtcct gtcgaggcgc ccgggacgac ggaacctagc gtgctcgaat tggacgacga
     2281 cgtgccgggc tctgaagggg acgccgccga tgaactcccc ggtgggcctg gctgcgccga
     2341 gccagttttg gtcgccggca gtcgatcgac gacggccgcc acgatgagcc caccgagcag
     2401 cacgagcacc agcgacacga cgacgatgcg atcgcgaacc gaaccgggca atggcatgac
     2461 ggtcagatcg ccttgacggc cgcgaccagt ttcgagagcg agtctttcgc gtcgccgaag
     2521 aacaacgtgg ttttggggtg gtagagcagt gcgttgtcga tgccggcgaa gccgggtcgc
     2581 atcgagcgct tcatgaacac gacgtgggtg gcttcgtcga cgttcagaat cggcatgccg
     2641 tagatcgggc tgccggcgga gtcgcgggca gccgggttca cgacgtcgtt cgcaccgacc
     2701 acaacagcga cgtcggtcga actgaactcg gggttggcgt cgtccatttc tttcagttgc
     2761 tcgtagggaa cgttcgcctc ggcgagcaac acgttcatgt gtcccggcat gcgaccagct
     2821 accgggtgga ttgcatagaa cacctctacg ccacgcgact cgagtaagtc aacaagttcg
     2881 cggcaggtgt gctgggcttg cgcgacagcg aggccgtagc cgggaacaat cgcgactttt
     2941 cgcgcgtagg caaggagaat ggcgatgtcg tcggcgcctg cggacttcac cgcgcggtca
     3001 gtggagacca cctcagcggt cgagccggcg gtcgagccac cggagaacgc gccgaacagc
     3061 acgccggtga tcgagcggcc catggcgcgc gccatcaaca acgtgagcat cgttccggac
     3121 gcaccgacca gggtgccggc cacgatgagc agtgtgttgt cgagcacata tccggaggcg
     3181 gcgacggtca ggccggtgaa ggcgttgagc aacgaaatca cgacgggtac gtcggcgccg
     3241 ccgaccggca acacgaacag cacgccaagc ccgagggcga gcaccgtgca gatcacaagg
     3301 aacgtcacgc tgcccgacgc gtgacttgcg gtggcgacag cagcgatccc gcagccgacc
     3361 gcgccgaggc cgaccaaggg cgtgacgacc tgcgcgccgg gcaccaagat cggccgcccg
     3421 gtcatcagtt cctggagctt ggcgaaggtg atcagtgatc cggtgaagct gacactgccc
     3481 acgatcgcgg tgaacgcgac ggcga
//
LOCUS       DATCBJ010000259         7932 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_189562, whole genome shotgun sequence.
ACCESSION   DATCBJ010000259 DATCBJ010000000
VERSION     DATCBJ010000259.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 7932)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7932)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7932
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_189562"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..432)
                     /locus_tag="VII50_05315"
     CDS             complement(<1..432)
                     /locus_tag="VII50_05315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5050298.1"
                     /translation="MIKRVVAWAGGALLLTLIAVSAPLERAAHQSSTANDVAGLVAFV
                     AFGGVGVVVAVRQPRNVMGWILLGIPVCLVLNTDMSAYAAIDYRVSPGRLPLGPLAVQ
                     LQPSWAPGVVLLGLAILLFPDATLPSLWWRRSVVAFLALSAV"
     gene            575..1102
                     /locus_tag="VII50_05320"
     CDS             575..1102
                     /locus_tag="VII50_05320"
                     /inference="COORDINATES: protein motif:HMM:TIGR02983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SigE family RNA polymerase sigma factor"
                     /protein_id="HEY5050299.1"
                     /translation="MAVVDAEFADFVRANERSLLRLGWLLMADRHAAEDLVQTALERT
                     MPKWASIRHDDPMAYVHRVMLNTRTSWWRRHRGRESLTSDVSDRGVDDATDAFAERDR
                     LTIALRSLTAGQRKVVVLRHYEDLSEAQVAALLGCSIGTVKSQNARALARLRLALAEP
                     THDTQAALATGTEGP"
     gene            1099..1947
                     /locus_tag="VII50_05325"
     CDS             1099..1947
                     /locus_tag="VII50_05325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5050300.1"
                     /translation="MTDERDPLVADALRRLAGAAPQPRPDRLQAVVAGVRRRRQVRTT
                     TSALTAAVLVVAAAVGVHVWTTPASDTTGPGPTPPAVSASPDAAASSALASQVPVASA
                     SASVIPVIAPTPTAMPTVTAKGLVATVTTPASVVDGDPAKVTMTVTNKSSVTTRGGSF
                     TVVSAYAEAAGQLAGFEFSGYTTSCKSVSGGLVCDLGTLKPGQSEQFWQAITATGPAD
                     QILFDVKWTNNAGVSTTFHRVVRVVPGVPSFELSTPPSSVLPTPPSSSPPPASGSPSS
                     PKPSSS"
     gene            1996..2832
                     /gene="fdhD"
                     /locus_tag="VII50_05330"
     CDS             1996..2832
                     /gene="fdhD"
                     /locus_tag="VII50_05330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009081138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HEY5050301.1"
                     /translation="MGRVTQRRQVLHLDGGVAAQRPDTLAAEEPLEIRLGGTSMAVTM
                     RTPGDDFDLVAGFAWTEGLLTDPDHLAALRYCAGADETGRNTYNVIDLVLSDEAPLVD
                     ASVARNFYATSSCGVCGKASIDAVTVRSRFDLHIDRTSVTAAILSLLPDKLREAQRVF
                     DRTGGLHAAGLFTPDAELLVLREDVGRHNAVDKVVGWALREKRLPLAGTVLMVSGRAS
                     FELVQKAYVAGIPVLAAVSAPSSLAAEFADSAGMTLIGFLRGTSMNVYAGADRVALTV
                     PA"
     gene            2829..3140
                     /locus_tag="VII50_05335"
     CDS             2829..3140
                     /locus_tag="VII50_05335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5050302.1"
                     /translation="MSPRRTRPTRRSTSNVDADEDGDTGLTGGFQFVEVDARGEEWVV
                     RRLTGASSTKTYRCPGCNQEIRPTTPHVVVWPSLAVDMRRHWHTPCWNSRSRRSSREL
                     R"
     gene            3289..5397
                     /locus_tag="VII50_05340"
     CDS             3289..5397
                     /locus_tag="VII50_05340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5050303.1"
                     /translation="MPLGVGFLVLCACSPGPPSAAQRSAMPSQSSLSSSTNPSAAGSP
                     TSVAAPSSTEPGSPPITQPPSSLVDVDSVAVPQWSSTGCPPSGLELQQSVTGRFSQCL
                     QVGGVPGGDYAIVLEQVLSPPADVAPASPAVPGLQLTASPASGPPGTAVSITGTVTTP
                     LSPRPGGVSLCWDGCPGGLAYPGETLQWLSPTTFRTTIVVPQAPWVTAWPPRVVPLTS
                     GTYSIGVQCLQEAHGCGLGGAEATTSFRLDVPAADAPSWCRTADSCAQMTVSPAHALP
                     GQQITVTGFAPVAAMSGDGQPLAYQLQVIPAPTSGPSVQFQSLGKAEATEAYFGYGAL
                     TVMASPTFASINSTSWRLQASDGWQPVTENPATPSTTAWCGSGAITIDAAGRTSTIPT
                     GGAAQAALTAMGFPSQPGESPTALTCDTLALLGNGVAGAPAVVAGFGVEPPDQEPLFA
                     DVALLTRDGGKSWTPLPTPRGVKPDSFGGFRYGGDAVTALFAPSGTTDPAPAVEATSD
                     AGRTWIPAHMTCPTSGPCITFGAFQPGNCAKDGGYQSVIASTDGGHHWAVTSLDQNLD
                     ACWPAQLLTDADGSEVLVDSSSTFTVERSTDGGRTWADIDIPPVPNRPVGDGYDGAVI
                     GLPGGSLLATSQDTADAWMLLRAGATTWCAVATPDTTNQDSVFYGGPRAIGDQLWWLT
                     WANDSTGTTAHHVALAELSC"
     gene            5510..6445
                     /locus_tag="VII50_05345"
     CDS             5510..6445
                     /locus_tag="VII50_05345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5050304.1"
                     /translation="MAASSPDTAPVVASSVLGSFGPSASGVVLAIDDSGRFRIEDLGS
                     ATVLHTLSIATVSQGPSLIASARGGWVVSYAPAPGNGDSLPAARLAFVARSGAVTPFG
                     PSFPQSSPISGLAVSHDGTRVAIALMQVQPGATPAEIRVMAMPGHASPTRSWPVDDVD
                     VNEMMSLSFAPDGRRLSYIAGSQTGAGIGGDPATLDTSSDAIQAPTSSTWLDNTNQQC
                     LPDGASWLGTSGKFAVVADCPPNDVLKEVNPVTGARTGSDVALPFYGCLDANIHPAAD
                     ARAMLISRCDRVYLVADGAATALDPHLVDAAWADT"
     gene            complement(6481..>7932)
                     /locus_tag="VII50_05350"
     CDS             complement(6481..>7932)
                     /locus_tag="VII50_05350"
                     /inference="COORDINATES: protein motif:HMM:NF012766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HEY5050305.1"
                     /translation="RGSGGDGAAKWAEMLFDGAMTADETFATTNYDAAVSPALTAFMR
                     EGWAPDPAPAIEPSEVAPYSAKRRNAVSAAFPGEVIVVGAGTPSIRNGDVFHPFRANS
                     DFVWLTGAAEPDAVLVMTPDAGSPEGHVSTLYLRFSASRHESEEFYRDRFHGELWTGR
                     RPGIAETQASLGLTTRPLGELDGVLHALSTAGAPIADDALVATTLADLRLVKDDWEIA
                     QLSEAVAASVRGFEDVVRELDAARSTSERWIEGTFARRARVEGNDVGYGSIAAVGEHA
                     CILHWTRNDGPVRDGDLLLLDAGVENAALYTADVTRTIPISGRFSEPQRRVYQLVYDA
                     QTAAMNAVRPGAHFRDYFRAAHEVLAKGLVEWGILPARAQDVDGPDGDLHRRYTLHGT
                     GHMLGLDVHDCAQARKENYHEGVLQAGYVLTVEPGLYFQSDDLTVPPELRGIGVRIED
                     DVVVTHDGCRNLTEALPRDPDAVEAWMAKLLAG"
BASE COUNT         1262 a         2653 c         2680 g         1337 t
ORIGIN      
        1 caccgcggag agagcgagga aggccaccac cgatcggcgc caccagagtg acggcagtgt
       61 cgcgtccgga aagagcagga tcgctagccc gagcaagaca acgcctggag cccacgacgg
      121 ctggagctgg acggcgagtg gtccgagcgg caatcggccg gggctgacgc ggtagtcaat
      181 cgccgcgtat gccgacatat cggtgttcag cacgaggcac accgggatcc cgagcaggat
      241 ccatcccatg acatttcggg gttgacgcac cgcaacgaca acaccgacgc cgccgaatgc
      301 cacgaaggcg acgaggccag cgacgtcgtt cgccgtgctg gactggtggg ccgccctctc
      361 cagcggagcg cttaccgcga tgagcgtgag cagcagggcc ccgccagccc aggcgaccac
      421 ccgcttgatc atcgcccgca tccttcgtcg ccgcgcaggt ccctcgctcc tagacgctac
      481 aagcaccgcg ttccgacatc gttacggacg ggcctgtgac aaccttgccg ccaccgggtg
      541 cgtctacccg ggcgaatcag cggaaggggt cgcggtggct gtcgtcgacg ccgagttcgc
      601 tgacttcgtg cgcgcgaacg agcggtcgct gctccggctt gggtggctgc tcatggccga
      661 tcgacacgcc gctgaggatc ttgtgcagac cgccttggaa aggacgatgc cgaaatgggc
      721 gtcaattcgg cacgacgacc cgatggccta cgtgcaccgg gtgatgctga acacccgaac
      781 atcgtggtgg cgccgccatc gtggtcgtga gtcgctgacg agcgacgtca gtgatcgcgg
      841 tgtcgacgat gcgacggacg cgttcgccga acgcgacagg ctgacgatcg cgctgcgcag
      901 cctcactgcc gggcaacgca aggtcgttgt cctgcggcac tacgaagact tgtcagaggc
      961 ccaggtggcc gcgctgcttg ggtgctcgat tggaaccgtg aagagccaaa acgcccgcgc
     1021 gctagcgcga ttgcggctcg cgctagccga gccgacccac gacacgcagg cggctctggc
     1081 gactgggacg gagggcccat gaccgacgaa cgcgatccac tcgtcgctga tgccctccgt
     1141 cggcttgctg gcgcagcacc gcaaccgcgg ccggaccgcc tgcaagccgt tgtcgccggt
     1201 gtgcgtcggc gtcggcaggt gcggacgacc acaagcgccc tgaccgcggc cgtgttggtg
     1261 gtcgcggccg cggtcggggt acacgtctgg acaacgccgg cctcggatac gacaggaccc
     1321 ggtccgactc cgccagccgt cagtgccagc ccggatgccg ctgcgagtag cgcgctcgcc
     1381 tcacaggtcc ccgtcgcctc ggcgtccgcg tcggtgatac cggtgattgc accgacgccc
     1441 acggccatgc ccaccgtcac cgcgaagggc ttggtcgcga cggtcaccac tccggcgtcc
     1501 gtcgtcgatg gcgacccagc caaggtcacg atgacggtga cgaacaagag ctcggtcacc
     1561 acgcgcggtg gcagcttcac cgtcgtaagc gcctacgccg aggcggctgg gcagctggcc
     1621 ggtttcgagt tcagcggata tacgacgagt tgcaagtcgg tgagcggcgg gctcgtttgt
     1681 gatctcggca cgctgaagcc cggtcagagc gagcagttct ggcaggcgat caccgcgacc
     1741 gggccggctg accagatcct gttcgacgtg aagtggacga acaacgcggg cgtctcgacg
     1801 acgttccacc gggtcgtgcg ggtggtgccg ggggtgccgt cgtttgagtt gtcgacccca
     1861 ccgtcctccg tgttgccaac cccgccgtcg agcagcccgc caccagcgag tggttcgccg
     1921 agttcgccga agccgtcgtc cagctgagtc tcagcgcgac aaccgcaggg gctcggcgcg
     1981 gatacgctgg ttcgtatggg acgggtcacg cagcggcgtc aggtgctgca cctcgacggc
     2041 ggggtcgcgg cccaacgccc ggacacgctg gcggctgagg agccgctgga gatccgtctc
     2101 ggtggtacgt cgatggcggt cacgatgcgc acaccgggcg acgatttcga tctcgtcgcg
     2161 ggcttcgcgt ggaccgaagg tctgctcacc gaccccgacc acctggcggc cctgcgctac
     2221 tgcgcgggcg ccgacgagac cggtcgcaac acctacaacg tgatcgatct cgtgctctcc
     2281 gacgaggcgc cgttggtcga cgcgagtgtg gcgcgaaact tctacgcgac cagctcgtgc
     2341 ggagtctgcg gcaaggcgag catcgacgcc gtgacggtgc gttcccgctt cgatctgcac
     2401 atcgaccgga cgtcggtgac tgcggcgatc ttgtcgttgc tacccgacaa gctgcgcgag
     2461 gcgcagagag tcttcgatcg caccggcggc ttgcatgccg ctggcctctt cacgccggac
     2521 gccgaattgc tggtgttgcg tgaagacgtc ggccggcaca acgcagtcga caaggtggtc
     2581 ggctgggcgc tgcgcgagaa gcgactgccg ctcgccggta ctgtgttgat ggtcagcggc
     2641 cgtgcgtcgt tcgagttggt gcaaaaggct tacgtggcag gcattccagt gctagctgca
     2701 gtgtcggcgc cgtcgagctt ggccgccgaa tttgccgact cggccggcat gacgctgatc
     2761 gggttccttc gcgggacgtc gatgaacgtt tacgcaggag ccgatcgcgt ggcactgacg
     2821 gtgccggcgt gagcccgcgc cgaacccgac cgacccgccg atcgacgtcc aatgttgatg
     2881 ctgacgaaga cggcgatacc ggcctgaccg gtggattcca gttcgtcgag gtcgacgcgc
     2941 gtggcgaaga gtgggtcgtg cggcgcttga ccggcgcctc gtcgaccaag acatatcgct
     3001 gccctggctg caatcaggag atccggccga cgactccaca tgtggttgtg tggccgagtc
     3061 tcgcggtcga tatgcgtcgg cactggcaca caccgtgctg gaacagccga tcacgccgct
     3121 cgtcacgcga gctgcgctag ccgctactac ggcaaccgcc cgcgtgccta gctgcactgg
     3181 ccggtgagcc aaccgtttgc tggcctgtgg cgtcctcacg gcatggatct tggcgcgcgc
     3241 gggggaactc ccgctctggc gactcgccga ctgctttggt ttggtctcgt gccgctgggg
     3301 gtcggctttt tggtgctctg cgcgtgcagt ccggggccgc cgtcagccgc gcagcgtagc
     3361 gcgatgccct cgcagtcgtc gttatcgtcg agcacgaatc cgtcggcggc cggatccccg
     3421 acctcggtgg cggcgccgtc gagcacagag ccaggatcgc cgccgatcac gcagccacct
     3481 tcgtcgttgg tcgacgttga ttcggttgcc gttccgcaat ggtccagcac cggttgccca
     3541 ccgtcgggct tggagttgca gcagtcggtg acgggtcggt tttcccagtg cctgcaggtt
     3601 ggcggggtgc ccggtggcga ctacgccatt gtgcttgagc aggtgttgtc gccgccagcc
     3661 gatgtggcgc cggcgagtcc tgcggtgccg gggttgcagt tgaccgcctc gcccgcatct
     3721 ggaccgcccg gtacggcggt tagcatcacc gggacggtca ctacgccgct atctccgcgg
     3781 ccagggggcg tctcgctgtg ttgggacggg tgccctggcg gcctggctta tccgggcgag
     3841 acgttgcagt ggttgtcgcc gacaacgttt cgaaccacga tcgtcgtgcc gcaagcgccg
     3901 tgggtgaccg cgtggccgcc ccgcgtcgtg ccactgacat cgggcacgta ctcgatcggc
     3961 gtccaatgct tgcaggaagc tcacggctgt gggctcggtg gcgcggaggc gaccacgagt
     4021 tttcggttgg acgttcccgc ggcggacgcg ccgagttggt gccgcactgc agattcgtgc
     4081 gcgcagatga cggtgtcgcc cgctcacgcg ttgcccggtc agcagatcac cgtcacggga
     4141 tttgcgccag tggccgcgat gagcggtgac ggacagcccc tcgcctatca gctacaagta
     4201 atacccgcgc ccacaagcgg ccccagtgtt cagttccagt cgctcggtaa agccgaagca
     4261 acggaggcat acttcgggta cggcgccctg accgtgatgg cctcgcccac cttcgccagc
     4321 atcaacagca ccagttggcg gctacaggca agcgacggtt ggcaacccgt caccgaaaat
     4381 ccagcgacgc cgtcgacgac cgcgtggtgc ggcagcggcg cgatcaccat cgacgccgct
     4441 ggtcgcacca gcacgatccc aaccggcggg gcggcacaag cggcgctgac ggcgatggga
     4501 tttccgtcgc agccaggcga aagccccaca gcgctcacct gcgacacgct ggcgctgctc
     4561 ggaaacggag tcgccggtgc accggccgtg gtggctgggt tcggggtcga gccgcccgac
     4621 caagaaccgc tgttcgccga cgttgcgctc ctcacccgcg acggtgggaa gagttggacg
     4681 ccgttgccga cgccgcgagg tgtcaaaccc gacagcttcg gtgggttccg ctacggaggc
     4741 gacgccgtga cagcgctctt cgctcctagc gggaccaccg acccggcgcc ggcggtggaa
     4801 gcaaccagcg acgctgggcg cacctggatc cccgctcaca tgacctgccc caccagcgga
     4861 ccgtgcatca ccttcggcgc gttccaacca ggaaactgtg cgaaggacgg cgggtatcaa
     4921 tcagtcattg cctccactga cggcggccac cactgggcag tgacgtctct cgatcagaac
     4981 ctcgatgctt gctggccggc gcagctactc accgacgccg acgggtctga ggttctcgtc
     5041 gacagctcct caacgttcac cgttgaacgc tccaccgacg gcggccgaac gtgggccgac
     5101 atcgacatcc cgccagtgcc caaccgacca gttggcgacg gctacgacgg cgcagtcatc
     5161 ggcttacctg gcggatcgtt attggcgacg agccaggaca ccgccgacgc atggatgctg
     5221 ttgcgcgcag gcgcgaccac ctggtgtgcc gtcgcaaccc ccgacaccac caaccaggac
     5281 tccgtattct atgggggtcc gcgcgccatc ggcgaccagc tctggtggct cacctgggcg
     5341 aacgacagca ccggcacgac cgctcatcat gtcgcgctcg ccgagctcag ctgctgagca
     5401 tccgatgcgg acgttgcgac aaacacggca gtggtcagcg atcgcgctga ccagcgccac
     5461 cgtcgccgta tcgatgttgg ctgcgggatg cacgtctggg gcacgtccgg tggcggcatc
     5521 ctcgcctgat accgcgcccg tcgtcgcctc gtccgtgctt ggctcattcg gcccgtcggc
     5581 gtctggggtg gtactggcga tcgacgacag cggccggttc cgcatcgaag acctagggtc
     5641 tgcaaccgtg ctgcacacat tgtcgatcgc gacggtgtcg caggggccgt cactgatcgc
     5701 gtcggcacgc ggcgggtggg ttgtgtccta cgcgccggcg ccgggcaacg gcgattcgct
     5761 tcccgcggcc cggctcgcgt tcgtcgccag gtcgggtgcg gtgactcctt tcggcccgtc
     5821 gttcccgcag tcgtcaccta tcagcggact tgctgtcagc catgacggaa cccgcgttgc
     5881 gatcgctttg atgcaagtcc agccgggcgc gacgcctgct gagatcaggg tcatggcgat
     5941 gcccggacat gcgagcccga cccggtcgtg gccagtggac gacgttgatg tcaacgagat
     6001 gatgagcttg tccttcgcgc cggacggtcg gcggctgagc tacatcgccg gcagccagac
     6061 cggggccggt atcggcggcg acccggcgac cctcgacacc tccagcgacg cgatacaagc
     6121 acccacctcc tcgacctggt tggacaacac gaaccagcag tgcctgcctg acggcgcgag
     6181 ttggctcggg acgtccggta agttcgccgt ggttgcggac tgcccgccaa acgacgtgct
     6241 gaaggaggtc aatccggtga caggtgcacg caccggcagc gacgtcgcgc tgcccttcta
     6301 cggctgtctg gacgcgaaca ttcaccccgc cgccgacgcg cgcgcgatgt tgatcagccg
     6361 gtgcgaccgg gtctacctgg tggccgacgg cgcagcgacc gcgctcgatc cgcatctcgt
     6421 cgacgccgct tgggcagata cctgaacccg ccttttgcag tggtcggtgg ctgggggacg
     6481 tcaaccggca agcagtttcg ccatccatgc ctcgactgca tcggggtcgc gagggagtgc
     6541 ctcggtgaga ttgcggcatc cgtcatgcgt gacgacgacg tcgtcttcga tccgaacgcc
     6601 gatgccgcgc agctccggcg gcacggtgag gtcgtcggac tggaagtaga gaccaggctc
     6661 gacggtaagc acgtatcccg cctgtagcac gccctcgtgg tagttctctt tgcgtgcctg
     6721 cgcgcagtcg tggacgtcga ggccgagcat gtgtcctgtt ccgtgcagtg tgtatcgacg
     6781 gtgcaaatcg ccgtccgggc cgtcaacatc ttgcgcacgg gccggaagaa tcccccattc
     6841 cacaaggcct ttcgccaaaa cctcatgagc ggcgcggaag tagtcgcgga aatgcgcgcc
     6901 tggtctcacc gcattcatcg ctgcggtctg cgcgtcgtac accagctggt aaacccgtcg
     6961 ctgcggctca ctgaaacgtc cgctgatcgg gatcgtgcga gtgacgtcgg ccgtgtacaa
     7021 agcggcgttc tcgacgccgg cgtccagaag tagcaagtca ccgtcacgca cgggaccgtc
     7081 gttgcgcgtc cagtgcaaga tgcatgcgtg ctcgcccacg gccgcgatgc ttccgtagcc
     7141 gacgtcgttg ccctcgaccc gtgcgcggcg tgcgaaggtg ccctcgatcc agcgctcgct
     7201 ggtcgagcgg gcggcgtcga gttcacgcac gacgtcctcg aacccgcgca cgctcgcggc
     7261 gacggcctcg gacagttgcg cgatctccca gtcgtccttg acgagtcgca ggtctgcgag
     7321 cgtcgtggcg acgagcgcgt catcggcaat gggcgcgccg gccgtcgaga gcgcgtgcag
     7381 gacgccgtcg agttcgccga gcgggcgggt tgtcaatccc agggacgcct gcgtctctgc
     7441 aatgccggga cggcggccgg tccacagttc gccatggaaa cgatcgcggt agaactcttc
     7501 gctttcgtgg cgcgacgcgc tgaagcgtag gtacagcgtc gagacgtggc cctcgggcga
     7561 cccggcgtcc ggtgtcatca ccagtacggc gtccggctca gcggctccgg tgagccagac
     7621 gaagtcgctg ttcgcgcgga acggatggaa gacgtcgccg ttgcgaatgc tcggggtgcc
     7681 cgcgccgacg acgatcactt cccctgggaa cgccgccgag actgcattgc gccgcttcgc
     7741 cgagtaaggg gcgacctccg atggctcgat cgccggcgcc ggatcggggg cccacccctc
     7801 gcgcatgaat gcggtcagcg ccggggagac cgcggcgtcg taattggtgg ttgcgaacgt
     7861 ctcgtctgcc gtcatggctc catcgaacaa catttcggcc cacttcgctg ccccatcgcc
     7921 gcccgacccg cg
//
LOCUS       DATCBJ010000260         3226 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_103871, whole genome shotgun sequence.
ACCESSION   DATCBJ010000260 DATCBJ010000000
VERSION     DATCBJ010000260.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 3226)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3226)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3226
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_103871"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..566
                     /locus_tag="VII50_05355"
     CDS             <1..566
                     /locus_tag="VII50_05355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEY5050306.1"
                     /translation="SGVLELAVLGLLHESPMHGYELSKRLNTMLGAFRAFSYGSLYPC
                     LKQMLALGHVSADGESTGSRSKIVYRLTADGKEHLQNLLGEAGPSSWEDENFGVHFAF
                     FSQTDAEVRMRILEGRRSRLEERLDNVKTSVARTRERVDSYTLELQRHGLESVEREVR
                     WLTELIETERRADTAPSSAAASSSNTI"
     gene            610..1689
                     /locus_tag="VII50_05360"
     CDS             610..1689
                     /locus_tag="VII50_05360"
                     /EC_number="5.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005467059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004512 - inositol-3-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006021 - inositol biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-3-phosphate synthase"
                     /protein_id="HEY5050307.1"
                     /translation="MGSVRVAIVGVGNCAASLVQGVEFYKNADPEDRVPGLMHVQFGP
                     YHVHDIKFVAAFDVDGKKVGSDLADAIGASENNTIKFCDVPTTGVVVQRGHTYDGLGD
                     YYRDIIQESEEEPVDVVQALKDAKVDVLISYLPVGSEVADKFYAQCAIDAGVAFVNAL
                     PVFIASDPTWAKKFEDAGLPIIGDDIKSQVGATITHRVMAKLFEDRGVELLRTYQLNF
                     GGNMDFMNMLERKRLQSKKISKTQSVTSQIPHEMAKADVHIGPSDYVPWLDDRKWAYV
                     RLEGRSFGDTPLNLEYKLEVWDSPNSAGVIIDAVRAAMIAKDRGIGGPINSASSYFMK
                     SPPVQYSDDDARQAVADFIAGTVDR"
     gene            complement(1686..3161)
                     /locus_tag="VII50_05365"
     CDS             complement(1686..3161)
                     /locus_tag="VII50_05365"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HEY5050308.1"
                     /translation="MPASSSHPPSLTSTQRAAIDELTRAAPVVDELGQLFAHAGHDLA
                     LVGGSVRDALLGRLGNDLDFATSARPDEILRITKHWRDSKWESGIAFGTVGLLKDGVR
                     LEVTTYRYETYDTVSRKPDVVYGDSLDEDLRRRDFAVNAMALSVPDHVFHDPFGGARD
                     LGAHVLRTPGLPEDSFSDDPLRMMRAARFASQLRFDVDPPVAAAMTAMADRITKVSAE
                     RVRDELSKLMCTSEPRRGLALLVDTGLADLVLPELPKLRLESDEHFRHKDVYEHTLTV
                     VEQAIALEADSDIGAQRSDEPDLVLRLAALLHDIGKPATRRKEPGGRVSFHHHEVVGA
                     KMAKARLTALKYPKDVVADVSRLVELHLRFHGYGTGEWTDSAVRRYVRDGGPLLERLH
                     KLTRSDCTTRNKRRAAALSAAYDELEDRIEALREQEQIDALRPELDGYEISAVLGIPP
                     GPDVGRAYRYLLELRLDGGPIGKDAASAALRQWAAEHGVGA"
BASE COUNT          619 a         1063 c         1003 g          541 t
ORIGIN      
        1 gctccggcgt cctcgagctt gccgtcctgg ggctgctcca cgagtcaccg atgcacggct
       61 acgagctcag caagcgcctc aacaccatgc tcggcgcctt ccgcgcattc tcctacggct
      121 cgctctaccc gtgcctgaag cagatgctgg cccttggtca cgtcagcgcg gacggcgaat
      181 cgacggggag ccgcagcaag atcgtctacc ggctgactgc cgacggcaaa gagcacctac
      241 agaacctgct gggcgaggcc ggaccctcgt cgtgggaaga cgagaacttc ggcgtgcact
      301 tcgccttctt ttctcagacg gacgccgagg tgcggatgcg catcctcgaa ggccgccgca
      361 gccgcctcga ggagcggctc gacaacgtga agacctcggt cgcccgcact cgtgagcggg
      421 tcgacagcta caccctcgag cttcagcggc atggcctcga atccgtcgag cgcgaagtcc
      481 gctggctgac cgaactgatc gagaccgagc ggcgcgccga cactgcgccc tcaagcgccg
      541 cggcatccag ctccaacacc atctagcgcc accacttgta cggggattag cgaagaagga
      601 gcaccgctca tgggttcggt tcgggtagcc atcgttggcg tcggcaactg cgccgcgtcg
      661 ctcgtccaag gagtcgagtt ctacaagaac gccgacccag aggaccgggt gccgggcctc
      721 atgcacgtgc agttcggccc gtaccacgtg cacgacatca agttcgtcgc ggcattcgac
      781 gtcgacggta agaaggtcgg ttccgacctc gccgacgcga tcggtgccag cgagaacaac
      841 accatcaagt tctgcgacgt gccgacgacc ggtgtcgtcg tccagcgcgg gcacacttat
      901 gacggtcttg gcgactacta ccgcgacatc atccaggagt ccgaagagga gccggtcgac
      961 gtcgtgcagg cgttgaagga cgccaaggtc gatgtcttga tctcctacct gccggtcggc
     1021 tccgaagtcg ccgacaagtt ctacgcgcag tgcgcgatcg acgccggcgt ggcgttcgtc
     1081 aacgcgttgc ccgtcttcat tgccagcgac ccaacgtggg cgaagaagtt cgaggacgcc
     1141 gggctgccga tcatcggcga cgacatcaag agccaggtcg gtgcaacgat cacgcaccgc
     1201 gtcatggcca agctgttcga ggaccgtggc gtcgagttgc tgcgcacgta ccagctgaac
     1261 ttcggcggca acatggactt catgaacatg ctcgaacgca agcgcctgca gtccaagaag
     1321 atctcgaaga cgcagtccgt gacatcgcag atcccgcacg agatggcgaa agccgacgtg
     1381 cacatcggac cgtccgacta cgtgccgtgg ctcgacgacc gcaagtgggc ctacgtgcgc
     1441 ctcgagggtc gctcgttcgg cgataccccc ctgaatctgg agtacaagct cgaggtgtgg
     1501 gactcaccga acagcgccgg agtcatcatc gacgccgtcc gcgcagcaat gattgcgaag
     1561 gaccgcggca tcggcggacc gatcaactcg gcgtccagtt atttcatgaa gagtccgccg
     1621 gtgcaataca gcgacgacga cgcccgccag gcagtggccg acttcatcgc tggcacggtc
     1681 gaccgctaag ccccgactcc atgctcggcg gcccactgcc gcaacgcagc tgaggcggcg
     1741 tccttgccga tcggtccgcc gtccagccgc agttcgagca ggtaacgata tgcacggccg
     1801 acatcaggcc ctggcggaat gccgagcacg gcactgatct cgtacccgtc gagctcgggc
     1861 cgcagcgcgt cgatttgctc ctgctcacgc aaggcctcga tgcgatcctc aagctcgtca
     1921 taggctgcgg acaatgcagc cgcccgtcgc ttgttgcgcg tcgtgcaatc cgagcgggtg
     1981 agcttgtgca atcgctcgag caacgggccg ccgtcccgca cataccggcg tacagcagag
     2041 tcagtccatt cgccggtgcc gtatccgtga aaacgcagat gtagctcgac caaccgcgac
     2101 acgtcggcga ccacgtcctt tggatacttc agcgcggtca gtcgggcctt ggccatcttc
     2161 gctccgacga cctcgtggtg gtggaacgag acccgaccac ctggttcctt acgacgagtc
     2221 gccggcttac cgatgtcgtg cagcaatgcg gcgagccgca acaccaggtc tggttcgtcg
     2281 gagcgctgcg cgccaatgtc ggaatcagcc tctaatgcga tcgcctgctc caccaccgtg
     2341 agggtgtgct cgtagacgtc cttatgccgg aagtgctcgt cgctttccag tcgcagcttc
     2401 ggcaactcgg gaagtaccag gtcggcaaga ccagtgtcga cgagcagcgc aaggccgcgt
     2461 cgcggttccg acgtgcacat caacttggag agctcgtcac gtacccgctc tgctgacacc
     2521 ttcgtgatgc ggtcggccat cgccgtcatg gcggcggcta ccggcggatc gacatcgaac
     2581 cgaagctgcg acgcgaaacg agctgctcgc atcatccgca aagggtcgtc ggagaacgag
     2641 tcctcaggaa ggcccggggt ccgcagaaca tgagcgccaa ggtcacgagc gccgccgaac
     2701 gggtcgtgaa agacgtgatc cggaacgctg agagccatcg cgttcacggc aaaatcccgg
     2761 cgccttaggt cttcgtccag tgagtcgccg tagaccacgt caggtttgcg cgacaccgtg
     2821 tcgtaggtct catagcggta ggtcgtgact tcgagtcgca cgccgtcctt cagcaatccg
     2881 acggtgccga aggcgatccc ggactcccac ttgctgtcac gccagtgctt cgtgatccgc
     2941 aagatctcgt ccggacgcgc actcgtggcg aaatccagat cgttgccaag ccggccgagc
     3001 aatgcgtcgc gaaccgagcc tccgacgagc gcgagatcgt gaccggcgtg cgcaaacagt
     3061 tggccgagtt cgtcaacgac cggcgccgcc cgggtcagct cgtcgatcgc ggcccgttgg
     3121 gtgctcgtta gcgaaggcgg gtgggaactc gacgcaggca caggggtcaa gggtaggccc
     3181 aaccacgcga aaagccctga tcgggcacta cccgacgccc tggtcg
//
LOCUS       DATCBJ010000261         2650 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_486860, whole genome shotgun sequence.
ACCESSION   DATCBJ010000261 DATCBJ010000000
VERSION     DATCBJ010000261.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2650)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2650)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2650
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_486860"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(231..1229)
                     /gene="gap"
                     /locus_tag="VII50_05370"
     CDS             complement(231..1229)
                     /gene="gap"
                     /locus_tag="VII50_05370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011897301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HEY5050309.1"
                     /translation="MTVRVAINGFGRVGRTFFRIAQERGMEVVAVNDVTDVATLAHLL
                     TYDSTFGRLRGEVDHGDTWISVDGQRVTAVAERDPRRLPWKDLGVDIVIESTGYFRAR
                     DDAALHLEAGARKVLISAPGKGVDATIVLGVNEDTYDTRQHHVISNASCTTNCIAPMV
                     KVLDDAFGFARGFMTTIHAFTNDQVTLDSPHKDLRRGRTASVNIIPTSTGAARSVGIV
                     IPELAGRLDGVAIRVPVEDGSLVDLAAMLDQEVTADEVNAAFMNAADGLLKGIMRYTR
                     DPVVSRDVIGDPASCVFDSLLTQASGPLVKVFGWYDNEWGYTSRLVDLTEFVAARL"
     gene            complement(1226..1696)
                     /locus_tag="VII50_05375"
     CDS             complement(1226..1696)
                     /locus_tag="VII50_05375"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEY5050310.1"
                     /translation="MADRTDVAVKRIVVGVDGSPESKAALRWAARIAPTIGCSIDAIM
                     AWDYPASYGWVMAPDGWPIEEDSAATLQSIIDEVFGELRPVNLRAVCQQGAARTVLLD
                     TSKDAEMLIVGSRGHGGFSGLLLGSVSAACAEHAHCPVLVVHGEPCPNTKESAA"
     gene            complement(1753..2361)
                     /locus_tag="VII50_05380"
     CDS             complement(1753..2361)
                     /locus_tag="VII50_05380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5050311.1"
                     /translation="MELYWLPLGAGDAMPLVRWNGRIFEALVARRDQREPRDLYHSAL
                     VVGLDQNRFVIEMTPAWESSASARGVRGEGAVGLRRLRRLRLFRYEVRCWRGGTIKDG
                     AAAVESPRAISTDPIRTQLLLNMTRSFPTATWGRDELATGEMWNSNSLTSWLLACSHH
                     DTDTICPPTGGRAPGWDAGLRVAARGLGEASVLRGRRHLVAE"
BASE COUNT          509 a          867 c          807 g          467 t
ORIGIN      
        1 ccgatcgata cttgcggctg catgtcgaac tcgtcagcgg gcagcacctg ccaatcgccg
       61 actcgcattc aaatttcgcg ccacggcttc gtcgcgtcgc agacgcgcgg tcttcgtttc
      121 ttagttgagg gttcccacgc cgagcttcta cgcctggacg tcatgaagta gccacaaacc
      181 gcaccggcct cgagggtgaa acctcgaggc cggtccggag ttgtagcccc ctataaccgc
      241 gcggcgacaa actcggtgag gtcgaccaac ctgctggtgt agccccattc gttgtcgtac
      301 cagccgaaga ccttgaccag cggcccgctt gcctgcgtga gcaacgagtc gaatacgcag
      361 gacgccggat caccgatgac atcgcgcgag acgacggggt cccgcgtgta gcgcatgatg
      421 cctttcaaga gtccgtctgc cgcattcatg aacgcggcgt tcacctcatc agcggtcacc
      481 tcttggtcca gcatcgcagc caaatccacg agcgaaccgt cctcgacggg cacgcgaatc
      541 gcgacgccat cgagacgacc ggcgagttcg ggaatcacga tcccgaccga gcgcgcagcg
      601 ccggtgcttg tcggaatgat gttcaccgac gcggtccggc cgcggcgtag atccttgtgc
      661 ggcgaatcca gcgtcacctg atcgttggtg aaggcatgga tcgttgtcat gaagccacgg
      721 gcgaagccga aagcatcatc gagcaccttc accatcgggg caatgcaatt ggtcgtgcag
      781 gacgcgttag agatgacgtg gtgctggcga gtgtcgtacg tgtcttcgtt gacaccgagc
      841 acaatcgtcg cgtcgacacc cttaccgggc gccgagataa gaacctttcg ggcgccggcc
      901 tcgaggtgga gtgcggcgtc gtcgcgggcg cggaagtatc cggtcgactc gatgacgatg
      961 tcgacaccga ggtccttcca gggcaaacgg cgaggatctc gctccgcgac ggctgtgacg
     1021 cgctgcccat cgaccgaaat ccaggtgtcg ccgtgatcga cctcgccccg cagtcgcccg
     1081 aacgttgagt cgtaggtcag gagatgcgca agcgttgcca cgtccgtgac gtcattgacg
     1141 gccacgacct ccatcccacg ttcttgcgcg atacggaaga aggttcgtcc cacccgacca
     1201 aagccgttaa ttgctacccg gactgtcatg ctgcactctc cttggtgtta ggacacggtt
     1261 ctccgtgcac gacaagaacg gggcagtgag catgttcggc acatgcggcg ctcacggatc
     1321 ccaacagaag gccgctaaac ccgccatgcc ccctgcttcc gacgatcaac atctctgcgt
     1381 ccttgctggt gtcaagcagc acagttcggg cggctccctg ttggcagacg gcgcgcagat
     1441 tgacgggccg aagttcgccg aacacctcat cgatgatgga ttgcaatgtc gcggctgaat
     1501 cctcttcgat cggccaacca tccggcgcca tcacccagcc atagctcgct gggtaatccc
     1561 aggccatgat cgcgtcgatg ctgcacccga tcgtgggcgc gatgcgtgcg gcccaccgca
     1621 gcgccgcttt cgattccgga gacccgtcta ccccgaccac tattcgctta accgcgacgt
     1681 cggttcgatc cgccatagcc aacccttcca actctaagtt cctcgcgaga tgcaacaagc
     1741 ggtagggact aactactcgg ccaccaggtg ccttcgtcct cgcagcacgg atgcttcgcc
     1801 gaggccgcgc gccgcgaccc gcaagcccgc atcccacccg ggtgcacggc cgccagtcgg
     1861 cggacagatc gtgtccgtgt cgtggtggct gcacgcaaga agccacgaag tcagcgagtt
     1921 tgaattccac atctcgccgg tggcgagttc gtccctcccc caggtggcgg tcgggaacga
     1981 ccgagtcatg ttcagcagca gctgcgtccg gatcggatca gtgctgatag ctcgcgggct
     2041 ctcgacggcg gctgctccat ccttgatagt cccgccgcgc cagcaacgaa cctcgtaccg
     2101 gaagagtcga aggcggcgga ggcgccgaag gccaacggcg ccttcgcccc tgactccgcg
     2161 agcggaggcg ctgctttccc aggccggggt catctcgata acgaaacgat tctggtccag
     2221 cccgactacg agcgccgaat ggtagaggtc ccggggctcg cgctggtcgc ggcgggcgac
     2281 gagtgcttcg aagatccgcc cgttccaacg gacgagaggc atggcatcgc cagcgcccaa
     2341 cggaagccag tacagctcca cgcttgcgtg tcctcggcgc gtcgcggagc tcaaccacac
     2401 cgcttgatac tcagagcccc tgttatgggc tgcatctaca tgagaagcga attgccggag
     2461 aagcagccgc cggcgtagcc cgagcgcgag ggtcggtggg agttcatcga aggccctcac
     2521 gatgtgtgcg cgctatgcgc tcgtcgggca aggtcgcggc gaccaacgag aaggaacccg
     2581 agcccggcgc caaagtagat cggctgcaag acgctgactg agcgtatgca ggcaagctcg
     2641 acgatgatcc
//
LOCUS       DATCBJ010000262         2418 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_41414, whole genome shotgun sequence.
ACCESSION   DATCBJ010000262 DATCBJ010000000
VERSION     DATCBJ010000262.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2418)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2418)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2418
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_41414"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1..1473)
                     /locus_tag="VII50_05385"
                     /pseudo
     CDS             complement(1..1473)
                     /locus_tag="VII50_05385"
                     /inference="COORDINATES: protein motif:HMM:NF015155.4"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
     gene            1646..>2418
                     /locus_tag="VII50_05390"
     CDS             1646..>2418
                     /locus_tag="VII50_05390"
                     /inference="COORDINATES: protein motif:HMM:NF025168.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing protein"
                     /protein_id="HEY5050312.1"
                     /translation="MSHSSALQPTEAGRQRVGRLAALQLALRMVMALTDLPVATFTFV
                     VMVTLVSTSVGLIAAFLVGIPLLILTGMIARALANFERARIRLFLDIDIPRLPGRPRG
                     FKAALSDGPTWRAILYPVLHFPLAIATFSSTVAALSTSLAMISMPAWLGHVPSRHADL
                     GALTVADQRTAWLMAAVGVVIGVIGAGLTYGLTTAEGALAKALLGVSAAQLAKRVDAL
                     EGSRARVVDAAEAERRRIERDLHDGAQQRLVSLAMNLGRA"
BASE COUNT          390 a          791 c          805 g          432 t
ORIGIN      
        1 cttcaaagca tggacggacg tcgagtcgct gccgtcgccg ttcaacggaa tcgacacctc
       61 ggcaaccgtt cctgccgcat taacgcgcgt cgtcaacgcg gccgacatct gaccgctcgc
      121 cgccgccttc gcagacatgg cctgtagacc ggcccgcacc gcgggcgtgt tgacgttcgg
      181 tgctgccacg accacgaagg ccggttgtgg gccaccaggg aaggcggtcg atatccggtc
      241 gaacgtctgc acgaccggca ggttcttcgg caggtcggag ttgcccggaa gcgcggtctt
      301 catgctgaca agcggcaacg cgagcagcgc taacactccg ccggcgatca gcgccgaccg
      361 agccggacga cgcagcacga gtctgagcac ggcaccccac gcccggctct cgcgcttggc
      421 ccggcgcagc ttgtgcacga acggcacccg acccttttcg atgcggtcgc cgagcagtga
      481 catcaacgcg ggaagcacgg tcaccgagcc gagcacggcg atcgcgacga ccagcaccgt
      541 cgcttccgcg acacccttga aggtggcgtt accggtgagg aagagtccac caactgcggc
      601 gaccaccgtg agtcccgaga cgaggatcta tcgtcccgat gtggcggccg cgatgcgcag
      661 cgcctgtccc gggctgttgc cgcgtgctcg ctcttcgcgc tcgcgcttaa ggtagaagag
      721 cgtgtagtcg acgccgacgg cgagaccgac taggagcatc accgaggtcg cggtgccatc
      781 gatgtgcacg gcgcggctgg tcagcgccag cagtccaccg gccgccatga aggcggtgag
      841 cgcgagcccg accggcacca ccgcggcgac gagtgaaccg aagaccgcga gcaggatccc
      901 gagggtcaag gggatcgaca gcatctcggc gcgcttgaag tccttgccga gcgtcttgtt
      961 gagcgccttc gcggcgctcg catcgccgac ttcttcgatg cgaagtgacg agttcgcctg
     1021 ctgcacccgt gcgactgcgt caaggactgg ttgcactcgc gattcggcag tgttggcgtc
     1081 gccggtcatg tcaaacgtca cgagcatcga atgcccgttg tgcgacacgt aggactgccc
     1141 gtccgtgtcc agcggtgagt gcaggttgtc gatgagcttc gtcgagctca ccgccgtgat
     1201 gacttgtgcg acggcagcgc gcaagccggc gtcgtccggc tggccatttc tcgtttgcac
     1261 gagcacattt tctgaagcgg cgtccggaaa accggacgag ctcaggagct gctcggcctt
     1321 cgcggactgc ccgttggacc cttcgacgtt ggtgatctgc ttcgtccctg acgagccgat
     1381 gaccagtgcc gccatgacga agaccagcca tccgatgatg gccgtcttgc ggtggccggc
     1441 gctccaggcg cccatgcgtg ccgcgacgtt catggtgagt tctcctctgt cgataggtcg
     1501 gctggtattg agaaacgaca gtggcggacg gctgtacctg gtgtcggttg ggcaggcgac
     1561 cgtattcggg gtggggatag cccccggtcg aagccgggag gtgacgggat gggtcggggg
     1621 cgtccgcgcc gctactttgg atcacgtgag ccactcatca gcgctgcagc cgaccgaggc
     1681 ggggcgtcag cgcgtcggcc gcctggcagc tctgcagctc gcgctgcgga tggtgatggc
     1741 tctgaccgac ctgccggtcg caaccttcac gttcgtggtg atggtcacgc tcgtgagcac
     1801 gtccgtcgga ctgatcgcag ccttcctcgt cggtatccct ctgttgatcc ttacgggcat
     1861 gattgctcgc gcgctggcga acttcgaacg cgcccggatt cgactcttcc tcgacatcga
     1921 catcccgcgg ctgccaggcc ggccacgagg gttcaaggcg gcgctgagcg atggcccgac
     1981 ctggcgcgcg atcctctacc cggtgctgca cttcccgctg gcaatcgcga ccttctcgtc
     2041 gaccgtcgcg gcgcttagca cctcactcgc gatgatctcg atgccggcct ggctcggcca
     2101 tgttccgtca cgtcatgccg atctcggggc gcttacggtc gcagatcagc gcacagcgtg
     2161 gttgatggcg gcggtcggtg tcgttattgg tgtcatcgga gctggactca cctatggact
     2221 cacgaccgcc gagggagcgc tagcgaaggc gttgctcggc gtcagtgccg ctcaactcgc
     2281 gaagcgcgtc gatgcgctcg agggcagtcg cgcgcgcgtc gtcgacgccg ctgaagccga
     2341 gcgtcgacgg atcgaacgcg acttgcacga tggcgcacaa cagcgcctcg tgtcgctcgc
     2401 catgaatcta ggccgggc
//
LOCUS       DATCBJ010000263         4356 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_153866, whole genome shotgun sequence.
ACCESSION   DATCBJ010000263 DATCBJ010000000
VERSION     DATCBJ010000263.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 4356)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4356)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4356
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_153866"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..283
                     /locus_tag="VII50_05395"
     CDS             <1..283
                     /locus_tag="VII50_05395"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEY5050313.1"
                     /translation="DDAADAIRRTNESRFGLCAGVLAATVEEGMLVARALEAGIIHVN
                     DQTVNDEPQMPFGGMKDSGWGRFGVGFAAEEFCDLQWITSRSEPRAFPF"
     gene            283..1461
                     /locus_tag="VII50_05400"
     CDS             283..1461
                     /locus_tag="VII50_05400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009071816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate 3-monooxygenase"
                     /protein_id="HEY5050314.1"
                     /translation="MRTQVGIVGAGPAGLLLARLLEAQGIESVVLERRDRHYVEHRVR
                     AGVLEHGSAQTLREAGVGTRMDAEGLPHGGTNLRFGLKTYRLDFAELTGRNVTVYGQQ
                     EVVKDLIAARLASGGELRFDVSEVALEGLDSDPRITFQYEGNRESVACDFVIGADGFH
                     GISRGCVAHVTASERTYPFAWLGILAEAPPSSAELIYARHANGFALHSMRSPTVTRMY
                     LQVAPDESLDEWPDDRIWAELHTRLADDSGFALTEGPIVSKGITPMRSFVATPMQCDR
                     LYLAGDAAHIVPPTGAKGMNLAVADVRLLARALGGYYTAGREDLLAAYSETASRRVWR
                     ATHFSWWMTTTLHKSPDDDPFDEALALAQLNYVVTSRAMATALAENYTGLPYEHDWFY
                     "
     gene            1559..2245
                     /locus_tag="VII50_05405"
     CDS             1559..2245
                     /locus_tag="VII50_05405"
                     /inference="COORDINATES: protein motif:HMM:NF006731.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047443 -
                     4-hydroxy-4-methyl-2-oxoglutarate aldolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-carboxy-4-hydroxy-2-oxoadipate
                     aldolase/oxaloacetate decarboxylase"
                     /protein_id="HEY5050315.1"
                     /translation="MPIVVRNTPRTDLAIVDALGELGVATIHEAQGRTGLLASRLRPI
                     YRPIKAAGSALTCEVAPGDNWAIHVAVEQAKPGDLLVVTPTSPCEDGYFGDLLATSLV
                     AHGVRGLVIDAGVRDIAFLTRIGFPVWSKAISAQGTVKETPANVQTPIVCGGVHIRPG
                     DVIVADDDGVVVVARADAAVVLEKARAREANEVTKRARLQAGELGLDIYDMRERLAER
                     GLRYVEGTDS"
     gene            2242..3327
                     /locus_tag="VII50_05410"
     CDS             2242..3327
                     /locus_tag="VII50_05410"
                     /EC_number="5.3.2.8"
                     /inference="COORDINATES: protein motif:HMM:NF016210.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalomesaconate tautomerase"
                     /protein_id="HEY5050316.1"
                     /translation="MSQTAVKCSVIRGGTSKGLYFHVEDLPADRETRDAVLLAAMGSP
                     DSREIDGMGGAHPLTSKVAVVAKSRRENADVDYLFLQVWPDRAEVSDNQNCGNLLAGV
                     GPFALEERLIEPTGDVTAVRIWMENTQTLAIAHVQTPAGKVRYDGAAAIDGVPGTHAP
                     IPIDFLDVAGSTCGALLPTGNAVDAINGVRVTCIDNGMPVVCIKADDLGLTGHESPSE
                     IEADADVLRRIEDIRLAVGPLMNLGDVTNKTVPKMTLVSSPQHGGAVNTRTLIPHRVH
                     DAIGVFGAVSVATACLVPGSVASEVAKNNDPAEVEIEHPTGFFTVSMDVEIVDGAVVV
                     KQAALLRTARLLMRGDVFVPRAVWAGR"
     gene            3324..4181
                     /locus_tag="VII50_05415"
     CDS             3324..4181
                     /locus_tag="VII50_05415"
                     /inference="COORDINATES: protein motif:HMM:NF015411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1932 domain-containing protein"
                     /protein_id="HEY5050317.1"
                     /translation="MKIALIGLGEVGRILAEDLADGNTLSAWDIAFGSPASPAARNAR
                     ELPVRVSTDAADAVRSANLVISAVTAANDLSASTDVASDIESGAWILDLNSASPAQKQ
                     QAAALIDTAGGRYVEAAVLSPIHPKRIGSPILLGGPHASDFVEVARALGFTGVEFFAP
                     SVGPASATKLCRSVVVKGLEALLMESMLTARTWGVENPVLDSLSNLLPAADWAALAEY
                     MIRRSVEHGERRSEEMVEAASTVANAGVEALMATATARRQALAGNQRDALVADDLGGL
                     LDAIRAART"
BASE COUNT          757 a         1409 c         1443 g          747 t
ORIGIN      
        1 cgacgacgcc gctgacgcaa tccggcggac caacgagtcg cgctttggct tgtgtgcggg
       61 cgtgctggcc gccactgtcg aggaaggcat gctcgtcgcg cgtgccctcg aggcgggtat
      121 catccacgtc aacgatcaga ccgtgaacga cgagccacag atgccgttcg gcggcatgaa
      181 ggacagcggc tggggtcggt tcggggtcgg ctttgcggcg gaagagttct gcgaccttca
      241 gtggataacg tcgcgaagcg agccgagagc cttcccgttc tgatgcgtac ccaggttggc
      301 atcgtcggtg ccggaccggc cggtctcttg ctggccaggc tgctcgaagc gcagggcatc
      361 gagtcggtgg tgctggagcg gcgcgatcgt cactatgtcg agcaccgcgt gcgtgccggt
      421 gtgctcgagc atggcagcgc tcaaacgttg cgcgaagcgg gtgtcggcac acgcatggac
      481 gccgaagggc tgccccatgg cgggacaaac ctgcgcttcg ggttgaagac gtatcgactc
      541 gactttgcgg agctgacggg ccgaaacgtc acggtctacg ggcagcagga ggtggtcaaa
      601 gacctgatcg cagcacgcct ggcgtccggc ggtgagctgc gattcgacgt cagtgaggtc
      661 gcgttggaag gtctcgattc cgacccgagg attaccttcc agtacgaggg caatcgcgaa
      721 agcgtggcgt gcgacttcgt gatcggcgcc gacggatttc acggcatcag ccggggatgt
      781 gttgcgcacg tcacggcttc cgagcggacc tatccgtttg cttggctcgg aatcctcgca
      841 gaagccccgc cgtcgtcggc cgaactcatc tatgcgcgcc atgcgaacgg gtttgcactg
      901 cacagcatgc ggtcgccgac cgtcacccgg atgtacttgc aggtcgcgcc cgatgaatcc
      961 ctcgacgagt ggccggacga tcggatctgg gccgagctgc acacgcggct ggccgatgac
     1021 tccggctttg ccctcaccga gggccccatc gtgagcaaag gcatcacgcc gatgcgcagc
     1081 ttcgtcgcca cgcccatgca gtgcgatcgt ctctacctcg cgggcgacgc cgcccacatc
     1141 gttccgccga ccggagcgaa gggaatgaac ctcgcggttg cagacgtgcg gttgctcgca
     1201 cgcgccctcg gcggctacta cacggccggc cgcgaggacc tcctcgcggc gtactccgag
     1261 acggcaagcc gccgggtctg gcgggcgacc cacttctcgt ggtggatgac cacaaccctg
     1321 cacaagtcgc cagacgacga tccgttcgac gaagcgctcg ccttggccca actcaactac
     1381 gtcgtcacct cacgcgctat ggccacggcg cttgcagaga actacaccgg actgccgtac
     1441 gagcacgact ggttctactg aaacggctag ttcaccgccc ctacaccgac acggaattcc
     1501 ttccgctcag cggaatacac tctggtttca acagatccac gctgaaagat gtgagttcat
     1561 gccgatcgtc gtccgcaata ctccccgcac cgacctggcg atcgtcgatg ccttgggaga
     1621 actcggcgtc gcgacgatcc acgaggcaca gggccggact ggcctactgg cgtcgcggct
     1681 gcgaccgatc tatcggccga tcaaggcggc gggcagcgcg ctcacctgcg aggtcgcgcc
     1741 cggagacaac tgggcgattc acgtggcggt cgagcaggcc aagcccggag acctgctcgt
     1801 cgtgacaccg accagcccct gcgaggatgg ctatttcggc gacctgcttg cgaccagcct
     1861 ggtcgcgcat ggcgtccgcg ggttggtgat cgacgcaggc gttcgcgaca tcgccttcct
     1921 gacccggatt ggttttccgg tgtggtcgaa ggcgatctcg gcacaaggca cggtgaagga
     1981 gacgccagcg aacgtgcaaa cgccgatcgt gtgcggcggc gttcacattc gtcccggcga
     2041 cgtgatcgtc gccgacgacg acggcgtggt ggtggtagcc cgcgcggacg ccgccgtagt
     2101 gctggagaag gctcgcgctc gcgaggcgaa cgaggtcacc aagcgcgccc ggctgcaagc
     2161 cggtgaactc ggtctcgaca tctatgacat gcgcgagcgt ctcgccgagc gcggtcttcg
     2221 ctacgtcgag ggcacagact catgagccag actgcagtta agtgctcggt gattcgcggc
     2281 ggaacctcga agggtctgta cttccacgtc gaggacctac cggctgaccg cgagactcgt
     2341 gacgccgttc tgctggccgc gatgggctca cccgattccc gtgagatcga cggcatgggc
     2401 ggggcgcacc cgctgacgtc gaaggttgcg gtcgtagcga agtcgagacg cgagaacgcc
     2461 gacgtcgact acctgttcct tcaagtctgg cccgatcgcg ccgaggtctc cgacaatcag
     2521 aactgcggaa acctgcttgc tggcgtgggc ccgttcgctc tcgaggagcg gttgatcgag
     2581 cccactggcg acgtgacggc ggtccggatc tggatggaga acacccagac gttggcgatc
     2641 gcccacgtgc agacaccggc cggcaaggtt cgctacgacg gagcggcggc aatcgacggg
     2701 gtgcccggca cgcatgcacc tattccgatc gacttcctcg acgttgccgg ctcgacgtgt
     2761 ggggcgctgc tacccaccgg caacgcagtc gatgcgatca acggcgtcag ggtcacgtgt
     2821 atcgataacg ggatgcccgt cgtctgcatc aaggccgacg atctgggcct gaccggccat
     2881 gagagcccga gcgaaatcga agcggacgcc gatgtgttgc gccgcatcga agacatccgg
     2941 ctggcggtag ggccgctcat gaatctgggc gacgtcacca acaagacggt gccgaagatg
     3001 accctcgtgt cgtctcccca gcacggcggc gccgttaaca cccgcaccct gatcccgcac
     3061 cgcgttcacg atgccatcgg cgtcttcggc gcggtctcgg tggcgactgc ctgcctggta
     3121 cccggttcgg tagcgagcga ggtcgccaag aacaacgatc cggccgaggt cgaaatcgaa
     3181 cacccgaccg gcttcttcac cgtctccatg gacgtcgaga tcgtcgacgg cgcagtggtc
     3241 gtcaagcagg cggcgctctt gcgaacggca cggctgttaa tgcgcggtga cgtcttcgtg
     3301 ccgcgcgcag tctgggcggg ccgatgaaga tcgccttgat cgggctcggc gaggtcggtc
     3361 gcattctcgc cgaggacctc gccgacggca acaccttgtc ggcgtgggat atcgcgttcg
     3421 gcagccctgc cagtccggct gcccgcaacg cacgcgagtt gccggtccgg gtgtcgaccg
     3481 acgctgccga tgccgtgcgg tcagccaacc tcgtgatcag tgcggtaaca gccgcgaacg
     3541 atctgtccgc atcaactgac gtagcgagcg atatcgaaag cggagcttgg attctcgatc
     3601 tgaactcggc atcgcccgcc cagaaacagc aagccgccgc gttgatcgac acggctggcg
     3661 ggcggtatgt cgaggctgcc gttctgtcgc cgatccatcc caagcggatc ggctcaccga
     3721 tcctgctcgg cggaccacac gcgagcgatt ttgtcgaggt ggctcgcgcc ctcggattca
     3781 ccggcgtcga gttcttcgcg ccgtctgtcg ggcccgcctc tgcgaccaag ctttgccggt
     3841 cggtcgtcgt caagggtctc gaggcactgc tgatggaatc gatgctcact gcacgtacgt
     3901 ggggcgtgga gaacccggtg ctcgattcgc tgtcgaacct cctgcccgcc gccgactggg
     3961 ccgcgctcgc cgagtacatg atccggcgct cggtcgagca cggcgagcgt cgcagcgagg
     4021 aaatggtgga ggccgcgtcg acggttgcga acgccggtgt cgaggcgctg atggcgacgg
     4081 caacggcgcg ccgtcaagcg ttggccggca accagcgcga cgccctcgta gccgatgacc
     4141 tgggtggcct cctcgacgcg attcgagcgg cccgcacatg atcattgact gtcacggcca
     4201 ctacacgacg gcgcccgacg cccataacgc atggcgcgat gcccaggtcg ctgcgtatga
     4261 cgccggagtc gctgcgccgt tgtgcccgag cattaccgat gggcagatcc aggagacgat
     4321 cgagaagaat cagctccgcc tgctcaccga acgcgg
//
LOCUS       DATCBJ010000264         2419 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_49311, whole genome shotgun sequence.
ACCESSION   DATCBJ010000264 DATCBJ010000000
VERSION     DATCBJ010000264.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2419)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2419)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2419
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_49311"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..425)
                     /locus_tag="VII50_05420"
     CDS             complement(<1..425)
                     /locus_tag="VII50_05420"
                     /inference="COORDINATES: protein motif:HMM:NF024852.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEY5050318.1"
                     /translation="MRVFVTGGSGWIGAAVVPELISAGHQVVGLARSEKSAAALTAAG
                     VEVQSGNLDDLDTLRNVADKSDAVIHLAFKHDLVYSGDFQGAAAADRRAVEAFGEALA
                     GSDRPFLIASGTLGLTQGTLATEADGRGPVNVGGSGPSVR"
     gene            545..1111
                     /locus_tag="VII50_05425"
     CDS             545..1111
                     /locus_tag="VII50_05425"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HEY5050319.1"
                     /translation="MSRWQPDAQGRLAQAALELYVEHGFEQTTVAEIANRAGLTERTF
                     FRYFADKREVLFGGAGALQELLVSTVAAAPDPMPAIDAIAVGLEAAAALLQQRRDYSL
                     QRQAAIVANAELRERELIKMASLAAALAAALRHRGVKDLAANLTAEAGIAVFKIAFER
                     WIDVTNERDFPALIQESMDELKAVTAGG"
     gene            complement(1086..1670)
                     /locus_tag="VII50_05430"
     CDS             complement(1086..1670)
                     /locus_tag="VII50_05430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5050320.1"
                     /translation="MDEAQELTDAQWQILLRRCPSRSFTVGDRAQARHGFTESWQERL
                     SRLGLAQITLDSVSINYRTPEAVMAEAEPVIRAVLPDANVPTSIRSTDVPAVHGLVSD
                     LGSILGAWLAEHPEGIACVIGTGRTQDGGCRAGAGRPRVRSLTPELSKGLEFDLVVLV
                     DPETFCEGIEGAVDRCVATTRATQQLVILQQSLP"
     gene            complement(1899..2207)
                     /locus_tag="VII50_05435"
     CDS             complement(1899..2207)
                     /locus_tag="VII50_05435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5050321.1"
                     /translation="MSTDDVKTLIETIADEADATAELPMPPGAAGTRPTKSVPVAVRL
                     APADVAAIEALADRLDVPMSSLLRGWILAALATNNEQTIATAVDRLAADVQRLREIVA
                     "
     gene            complement(2204..>2419)
                     /locus_tag="VII50_05440"
     CDS             complement(2204..>2419)
                     /locus_tag="VII50_05440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5050322.1"
                     /translation="NEALADVDGVWLDPDPKSTSGQSARVVGYSHTVAAVLVVILVHR
                     ENGGWWGANGWRANSSDARIYREGTPT"
BASE COUNT          423 a          796 c          790 g          410 t
ORIGIN      
        1 cggacgctcg gcccggatcc accgacgttg accggcccgc gcccgtccgc ctcggtcgcg
       61 agggtgccct gggtaagccc gagggttccg gacgcaatca ggaacggtcg gtcacttccc
      121 gcgagcgcct caccgaaggc ctcgacggca cggcgatccg cggccgcagc gccctggaag
      181 tcaccggagt agacgaggtc gtgcttgaac gcaaggtgga tcaccgcgtc ggacttgtcg
      241 gccacgttgc gcagggtgtc gagatcatca aggttgccgg actgcacctc tactccggcg
      301 gcagtcaggg cagcagccga cttctcggag cgagcaagcc cgacgacctg atggcccgcg
      361 ctgatcagct cgggaacgac agcggcaccg atccagccgg acccgccggt aacgaaaaca
      421 cgcatggaaa gaacctccag ataaaggcag accccggtac atggccggtc tcggtggtct
      481 atgtcagtta ctgtcatcac cgtagcaggt gatgtcagtt gctgtcatca actaccatca
      541 cgggatgagc cgatggcagc ccgacgcgca gggccgactg gcgcaagcgg ccctcgagct
      601 ctacgtggaa cacgggttcg aacagaccac ggtcgccgag atcgccaacc gagctggact
      661 caccgagcga acgttcttcc gctatttcgc tgacaagcgc gaggtcctgt tcggaggcgc
      721 gggcgcactg caagaactgc tcgtcagtac ggtcgccgct gcacccgacc ccatgccggc
      781 aatcgacgcg atcgccgtcg gcctggaggc ggccgccgca ctcctccagc agcggcgcga
      841 ctattcgctg cagcgccagg ctgcgatcgt cgcgaacgca gaactcaggg agcgcgagct
      901 gatcaagatg gcatcgcttg ctgcggcgct tgctgcggcg ctacggcacc gtggggtcaa
      961 ggacttggcc gccaatctga ccgccgaagc ggggatcgcc gtcttcaaga ttgcgttcga
     1021 gcgctggatc gacgtgacga acgagcgaga cttcccggcg ctgatccaag agtcgatgga
     1081 tgaactcaag gcagtgactg ctggaggatg acgagctgct gggtcgctcg ggtcgtcgcg
     1141 acgcagcggt cgacggcccc ttctatgccc tcgcagaacg tctctggatc gacgaggacg
     1201 acaaggtcga actcgagccc cttcgacagc tccggggtca gcgatctcac gcgggggcgg
     1261 cccgcaccag cccggcaccc gccgtcttga gtgcgaccag tgccgatgac gcaggcgatg
     1321 ccttcaggat gctcggcgag ccaggcaccg aggatcgagc caagatccga gacaagtcca
     1381 tggacggcgg ggacgtcggt actgcggatc gaggtcggca cgttggcgtc cgggagcacg
     1441 gcccggatca ccggctcggc ttccgccatg actgcttccg gcgtccgata gttgatgctc
     1501 acagagtcca gcgtgatctg ggccagcccg agccgcgaaa gccgttcctg ccacgactcc
     1561 gtgaacccat gcctggcctg tgcacgatcc ccgacggtga agcttcgaga cgggcagcgg
     1621 cggagcagta tctgccactg cgcgtcggtc agttcctggg cctcgtccac cacgatatgc
     1681 gcgcacgaac gtggtgcgct cggagggatt cgaaccccca gcctcttgat ccgtagattc
     1741 acgctgcgta gtcaaggaag ttcgccaaaa tctattcccg caggccaggg gctatcaggt
     1801 ttgaccctga acatcaacga actcccccgt ccgattactc gctgctacat ttactgctac
     1861 attttcagcc ccatttcggg cctcgcactc acttcatgtc aggcgacgat ttcgcggagg
     1921 cgttgaacgt cggcggcgag ccggtccacg gcggtggcga tggtctgttc gttgttggtt
     1981 gcgagcgcgg ccaggatcca gcctcggagc agcgacgaca tggggacgtc gaggcggtcg
     2041 gcgagtgctt cgatggcggc cacgtcggct ggggccaggc gcaccgcgac cggcactgac
     2101 ttggtgggcc gggtcccggc cgcgccgggc ggcatcggca gctcggcggt ggcgtcagct
     2161 tcgtcagcga tcgtctcgat cagtgttttc acgtcgtcgg tgctcatgtc ggggtccctt
     2221 cccggtagat ccgcgcgtcg ctgctgttgg cccgccagcc gttggctccc caccagccgc
     2281 cgttctcgcg gtgcaccagg atgaccacca agactgcggc cacggtgtgg gagtagccga
     2341 ccacccgcgc gctttgaccg gacgtgctct tggggtcggg gtctaaccac accccgtcca
     2401 catcggccaa cgcctcgtt
//
LOCUS       DATCBJ010000265         2002 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_229464, whole genome shotgun sequence.
ACCESSION   DATCBJ010000265 DATCBJ010000000
VERSION     DATCBJ010000265.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2002)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2002)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2002
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_229464"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..232)
                     /locus_tag="VII50_05445"
     CDS             complement(<1..232)
                     /locus_tag="VII50_05445"
                     /inference="COORDINATES: protein motif:HMM:NF020332.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase domain-containing protein"
                     /protein_id="HEY5050323.1"
                     /translation="MISIGKLTSVEQAVRYLREAIAHRQIEYYTARGESPGRWNGAGA
                     EALGLRGEVTDTDFAAVLGGRHPRTGEDLGKHW"
     gene            complement(622..1734)
                     /locus_tag="VII50_05450"
     CDS             complement(622..1734)
                     /locus_tag="VII50_05450"
                     /inference="COORDINATES: protein motif:HMM:NF033542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HEY5050324.1"
                     /translation="MQKGTAYIGLDVHKASIAAAIAGDDGKPPTLLGTIANDPAAVRK
                     LVAGVQHGFGELRVAYEAGPTGYGLHRQLSAIGIECRVVAPAQIPRRANDRVKTDRRD
                     ALMLARLLRSGDLDPIWVPDESHEALRGLVRARADAHADLLRDRHRLNNFLMTRGLHG
                     PVGARMSSARYDRWLETLHFERPAERIVFDDSRDVIRGGVQRERRIETALYECARDSR
                     HAGLIRALQALRGVGELTAITIVTEVGDFRRFATAPHLMAYVGLVPSEHSSGASQRRG
                     HITKAGNHALRHVLIESAHHARRTPQVSPLMQRRLADLPEPLVALSWRAQQRLHFRYR
                     QLGARLGRPRAVVAVARELVGFIWAVGTMWEGLPAA"
BASE COUNT          318 a          618 c          736 g          330 t
ORIGIN      
        1 gccagtgttt gccgaggtcc tcacccgtgc gtggatgtct gccgccgagc accgcggcga
       61 agtcagtgtc ggtcacctcg ccgcgcagcc cgagggcctc ggcgccggcg ccgttccagc
      121 gtcccggcga ctcaccgcga gcggtgtagt actcgatctg ccggtgggcg atggcctcgc
      181 ggagataccg caccgcctgc tcgacgctgg tgagcttgcc gatggagatc atcgcaagag
      241 tccgtgttgc acgagctcca gcgtaggggc gaaatgccta caggccgctt acattttcgc
      301 cgtggaagac tcgccgccac cgcaggtgta attgtctgct ctggaaggat ttgtcaagcg
      361 cgagccagtg ggtgatgtcg aagggaatgc gctgcgtgtt gtgtgcggcg tggtgaacgg
      421 cgggggcgag acgcgacgga ggcagataca gcgatggtca tcagtctgct cttcgcgggt
      481 cagcatgctg gtgccgcatg tcaggagatc cgaggccaag ctgccgtcga gtctgtgatg
      541 acgcgagtcg ggtcgcaaca catttacgcg ggtgctcggc atcactgcca ccccgttgcg
      601 gtcccgcccc cgtcgttcag gtcacgctgc tggcagtccc tcccacatgg tgccgaccgc
      661 ccagatgaaa ccgaccaatt cgcgggccac ggcgaccacc gcccgaggcc gtcccagacg
      721 tgcacccagc tggcggtagc ggaagtggag gcgctgctgg gcacgccagg agagcgccac
      781 caggggttcg gggagatccg ccaagcgtcg ctgcatgagc ggactgacct gcggtgtccg
      841 gcgcgcgtgg tgcgcggact cgatgagcac atgccggagc gcgtggttgc ccgccttggt
      901 gatgtggcca cgtcgctgcg acgccccgct ggagtgctcg gagggcacca ggccgacgta
      961 ggccatcagg tgcggcgcgg tggcgaagcg gcggaagtcg ccgacctcgg tgacgatggt
     1021 gatcgcggtg agctcaccga caccgcggag cgcctgcagg gctcgaatca agccggcgtg
     1081 acggctgtcg cgagcgcatt cgtagagggc ggtttcgatg cgtcgctcgc gctgcacacc
     1141 accacggatg acgtcacggg aatcatcgaa gacgatgcgc tcggcgggac gttcgaagtg
     1201 cagggtctcg agccagcggt cgtaacgggc cgacgacatg cgcgccccga caggcccgtg
     1261 cagcccgcgg gtcatcagga agttgttgag ccggtgacgg tcgcggagca ggtcggcgtg
     1321 ggcgtcggcg cgagcccgca ccaagccgcg gagcgcttca tggctctcgt cgggcaccca
     1381 gatcggatcg aggtcgccgc tgcgcagcag acgggcgagc atgagcgcgt cacggcgatc
     1441 ggtcttcacc cgatcgttag cgcggcgcgg gatctgcgcc ggcgccacca cgcggcactc
     1501 gatgccgatc gcgctcagct gacggtgcag cccgtagccg gtgggcccgg cctcataggc
     1561 aacgcggagc tcgccaaaac catgctgcac cccggcgacc agcttgcgga ccgctgcggg
     1621 gtcgttggcg atggttccca ggagtgtcgg cggcttgccg tcgtcgccgg cgattgccgc
     1681 cgcaatgctc gccttgtgta cgtccagacc gatgtacgcc gtacctttct gcatggaccg
     1741 gctctcccca cgtgtggctc ggtgcgatca cccggccggg ctcgcgcaac ccacgataac
     1801 cgtggccgag tggccggtcc ttccatgctg actgtgatgt gaaggagcga tgccgagaaa
     1861 gaagtgcgat ggatgcggag gaaagatgga gggagagaag gcggatggag gcgagtggcg
     1921 atgtggatga gggcgtttgc ggcggaggcg tggcttgggt agctgggcct gcgggttgcc
     1981 tgggcgtttt cggagccggc tg
//
LOCUS       DATCBJ010000266         2049 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_42897, whole genome shotgun sequence.
ACCESSION   DATCBJ010000266 DATCBJ010000000
VERSION     DATCBJ010000266.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2049)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2049)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2049
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_42897"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(318..659)
                     /locus_tag="VII50_05455"
     CDS             complement(318..659)
                     /locus_tag="VII50_05455"
                     /inference="COORDINATES: protein motif:HMM:NF017426.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF779 domain-containing protein"
                     /protein_id="HEY5050325.1"
                     /translation="MGAAARDSGPAVGATPAALEVIRRLESAHGPLAFFQSGGCCDGS
                     SAICLKDGELPLGPYDVRLGEIGGAPFYIDADQHERWGQPKFLIDVSPGAAMGFSLEG
                     LEGVHFISRTP"
     gene            complement(662..>2049)
                     /locus_tag="VII50_05460"
     CDS             complement(662..>2049)
                     /locus_tag="VII50_05460"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009083477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEY5050326.1"
                     /translation="AFCEVAHSGAQDIELALDAAHAAKDEWGQRSPAQRAVVLNDIAD
                     AIDENLEMLAVAESYENGKPVRETLAADIPLAADHFRYFAAAIRSEEGRISEIDEQTY
                     AYHFQEPLGVVGQIIPFNFPILMAAWKIAPALAAGNCTVIKPASPTPWSLLKLAEVIG
                     EIVPRGVLNIVNGPGAEIGKALASNKRIAKVAFTGETVTGRLIMSYAAQNLIPSTTEL
                     GGKSPNIFFEDVMAADDAFLDKAIEGLVLYAFNKGEVCTCPSRALIHESIYDEFMERC
                     LERIGQIKQGDPLDRETMIGPQVSTAQLEKIESYVKIGRDEGAEVLIGGHRPGLNGRL
                     AGGYYFEPTVLKGHNKMRVFQEEIFGPVLAVTTFRDEQEALEIANDTPYGLGAGVWTR
                     DGSRAFRMGRAIKAGRVWTNCYHHYPAHAAFGGYKNSGVGRENHRMMLEHYSQTKCLL
                     VSYDQNPMGFF"
BASE COUNT          343 a          688 c          657 g          361 t
ORIGIN      
        1 cagcggactg ctgaagtcgt gcacgaccgc agcgcggtag gtggtaggtg gcgcctctga
       61 ggtgtgtggc tgcggggtgg atgtggtggc catggcttcg tccttctttc tgggtggtcg
      121 acctctgtca cgccgttcta ggttgggttg ggcggcgcgg ggatctgcgc ctcgcaagca
      181 agacgatcag tgcgagtagg acgagcgagc cgagcacgat cgcgacgatc acgccgacac
      241 tcatgccgcc gtttgccggc cgtctgattt gagccgtagc cgcccatcac ggacggagcc
      301 ataagcgccg cccgtcgtca cggtgtgcga gagatgaaat gcaccccctc aaggccctcg
      361 agcgagaagc ccatggcggc gccgggcgag acgtcgatca ggaacttggg ctgaccccac
      421 cgctcgtgct ggtcggcgtc gatgtagaac ggcgcgccgc cgatctcgcc gagtcgcacg
      481 tcatacggcc cgagcggcaa ctcgccgtcc ttgaggcaga tcgccgagct gccatcgcag
      541 cagccgcccg actggaaaaa cgcgagcggc ccatgggcgc tctcgaggcg acgaatcacc
      601 tcgagagccg ccggggtagc cccaacagca ggcccactat cgcgagccgc agcccccatc
      661 tctaaaagaa ccccatcggg ttctggtcgt agctgaccag caggcacttg gtctgcgaat
      721 agtgctcgag catcatccga tggttctcgc ggccgacgcc ggagttcttg tacccgccga
      781 acgccgcgtg cgcggggtaa tggtggtagc agttggtcca cacccgaccg gccttgatcg
      841 cacggcccat ccggaacgcc cggcttccgt cacgggtcca gacgcccgcg ccgaggccgt
      901 atggcgtgtc gttggcaatc tcgagcgcct cttgctcgtc gcggaacgtt gtcaccgcga
      961 gcaccggccc gaagatctcc tcctggaaca cccgcatctt gttgtggccc ttgagcacgg
     1021 tcggctcgaa gtagtagccg ccggcaagac gaccgttcag ccccgggcgg tggccgccga
     1081 tcaggacctc ggctccctcg tcgcgcccga tcttgacgta gctctcgatc ttctcaagct
     1141 gcgcggtgga gacctggggg ccgatcatcg tctcgcggtc gagcgggtcg ccctgcttga
     1201 tctgaccgat ccgctcaagg caacgctcca tgaactcgtc gtagatcgac tcgtgaatca
     1261 gcgcccgcga cggacacgtg cacacctcgc ccttattgaa cgcgtatagc acgagaccct
     1321 cgatcgcctt gtcaagaaac gcatcgtcgg ctgccatcac atcctcgaag aagatgttcg
     1381 gcgactttcc gccaagctcc gtcgtcgagg ggatcaggtt ctgcgccgcg taggacatga
     1441 tcagccggcc ggtgaccgtc tcaccggtga acgccacctt cgcgatccgc ttgttggacg
     1501 cgagcgcctt gccgatctcc gccccgggac cgttgacgat gttcagcacc ccgcgcggga
     1561 cgatctcgcc gatcacctcg gcgagcttca agagcgacca cggcgtcggg ctggccggct
     1621 tgatcaccgt gcagttaccg gccgccaaag ccggcgcgat cttccacgcc gccatcagga
     1681 tcgggaagtt gaacgggatg atctggccca cgacaccgag cggctcctgg aagtggtacg
     1741 catatgtctg ctcgtcgatc tcggagatcc ggccctcctc ggagcggatc gctgcggcga
     1801 agtagcggaa gtggtcggcc gcgagcggga tgtcggcggc gagtgtctcg cgcaccggct
     1861 tgccgttctc gtagctctcc gccacggcga gcatctcgag gttctcatcg atcgcgtccg
     1921 cgatgtcatt caggacgacg gcacgctgcg ccggggaacg ctggccccac tcatccttgg
     1981 ccgcgtgcgc agcgtcgagc gccagctcga tgtcctgcgc accggaatgg gcgacctcgc
     2041 agaacgcct
//
LOCUS       DATCBJ010000267         4004 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_390285, whole genome shotgun sequence.
ACCESSION   DATCBJ010000267 DATCBJ010000000
VERSION     DATCBJ010000267.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 4004)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4004)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4004
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_390285"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1127..2191)
                     /locus_tag="VII50_05465"
     CDS             complement(1127..2191)
                     /locus_tag="VII50_05465"
                     /inference="COORDINATES: protein motif:HMM:NF012245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HEY5050327.1"
                     /translation="MGPRRNRAPSSPGRTALESFDVFEILEKAPHCVIVIDEQATVVY
                     QNAASRELDERMRAAHGEALLDAVRVAVADSGAGRRYPLTVLVSATTRSGHAHADVTV
                     DRIAGGFVVSWSDRTQVREGGQVVTKVATELLTTAESFTAFADMLASAVTEVSEQAYA
                     VATGSDQLTDSIQEIASSAAAAVGNTAGAVRSAQSATDLVARLGESSDRIGAVSRLIG
                     SIAGQTNLLALNATIEAARAGQAGKGFAVVASEVKSLARRSAEATEDITSTVTAIQAD
                     TAAAAAALEQIVHAIETMDSEQTTIASAVEEQSMTTAEIGARVAAVARAAQSSSSALI
                     GLREAAAVVAAKAHELRALV"
     gene            complement(2335..3582)
                     /locus_tag="VII50_05470"
     CDS             complement(2335..3582)
                     /locus_tag="VII50_05470"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY5050328.1"
                     /translation="MRPLRESPEYRRLWIAQSLSAMGNQMTNVAVPVQVYAMTHSSFA
                     VGAIGLAVAIPLIAVGLLGGSLADAVDRRKLVLLTSSGLALLSFVFALQAALNVRQLW
                     LLYVVMALQGCLFAIDQPARSTFIPRLLPPARIPAATALNQLSYQLSGTVGPLLAGVV
                     IAAAGLKAAYATDAATFTIAVYAIVRLRPMPPQGGGAPPGFAAVVEGLRWARRHRVIA
                     MIFIVDLNAMIFGMPRAVFPALAATHFGGGARTVGLLYAAPAIGGLIGAAFSGPLAHV
                     RRQGLAVLASVAVWGGSIAGFSMSRSLLLGAVLLAIAGAADMVSAVFRATILLQNVPD
                     ELLGRLSAVNFVVVSGGPRLGDLEAGGMAALTSPVFSAVSGGLACVVGVVLLGLAVPA
                     FARYRSGSPPPPTSTPDLPLAAS"
     gene            complement(3665..>4004)
                     /locus_tag="VII50_05475"
     CDS             complement(3665..>4004)
                     /locus_tag="VII50_05475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5050329.1"
                     /translation="VVVHGLDKLWSMRSQLDIPLEHVRGASFDPAIVHESKGWRGPGS
                     NVPGVITAGTFHQHGQLIFWDVHRGDNAVVIELEHDHFERLVVEVADPMATVGLIQRA
                     IAGRAGSADR"
BASE COUNT          700 a         1353 c         1300 g          651 t
ORIGIN      
        1 cgaaccgctg gtgcattact cgccaggggt cgacgtccgg ataggtcgcc cgcaccggct
       61 tcggtaattg cgcagcgccc acccttgctg tcgcctactg tttcgcggat cgcccactga
      121 gaggagttgg ccgccatgac cgtcgtcgcc gctgtgcccg ccctcctctc gctcggtatc
      181 gcctgaccgt cataccgagc gcccacctcg atttgcagga gcgctcaatc atgcctttcg
      241 cctcggcgtc cgagcacgcg aatgtttcgg cgtccgagga cgcctctgat tctccgtctc
      301 tagcggtgct cggctgggac tcttcccgtg ttgctgaact ggccttgctc tcctcggcgc
      361 gcggcgtcga ggtcgttgcc gcaaacgtcg acatcgtgtt cgtggtcgtg ccactcgtcg
      421 ggcggccgcg tccccggctg ttgcaacgct gtctcgcgag gcgtggcaat tcggcgccac
      481 acccgtcgtc ctgctcacga aggccgacat cgcaacggat gtgtctcagc gggttttgga
      541 cgcacaggcc gacgcggttg gcgctgacgt cttcgccgtg agcgcggcga ccggcgagag
      601 catcgatgca cttgtcggct atctccgtgc ggggcagacc gtcacgatga tcggacccgt
      661 caggtgcggg caagtcgacg ctcacaaacg ccttgggcgc agacatattc gctttgcaca
      721 ctggcgccgt gcgggacgac gggaaagggc cgccacacga cgaccgcgcg cgaagtcgta
      781 gtcctgccaa cgggcgcggt ggcgatcgac acgccgggac ttcgcggcct cgctttgtgg
      841 gatgccgaag aggaaatcgg cgtcgcgttc gcccgacgtg ctcgagttcg ccgccgattg
      901 ccgattgccg attcgccgat tgcgcgcagc acggcgagcc tggatgggcg gtcggccggg
      961 cgactaccga gggcgatctg ggcgccgcga ggttcgacga ctacgagaag ttgctgcgcg
     1021 agcaacgacg gttcgagacg cgtcgcgatg gtcgcgcccg tgccctagaa cacgcgcggg
     1081 ccgaggtctt ccacaaagct gtcaagcggc ggcgcatcgc taagagctag accaatgcgc
     1141 ggagttcgtg cgccttcgca gcgacgacgg cggccgcttc gcgcaatccg atgagtgccg
     1201 acgatgacga ctgagcagct cgcgcaacag ccgcgacccg ggcgccgatc tcggccgtcg
     1261 tcatgctctg ctcctcgacc gcgctcgcga tcgtggtctg ctctgaatcc atcgtctcga
     1321 tcgcgtgcac gatttgctcg agcgcggccg cggcggccgc agtgtccgcc tggatagcag
     1381 taacagtgct cgtgatgtct tcggttgctt ccgcgctgcg tcgagcgagg ctctttactt
     1441 cgctcgccac gaccgcgaat cccttgccgg cctggcctgc ccgagcggct tcgatcgttg
     1501 cgttcaaagc aagcaagttc gtctggccgg caatcgaacc gatcaagcgg ctgacggccc
     1561 cgatgcgatc gctcgactcg cccaagcggg cgacaaggtc agttgccgat tgtgccgaac
     1621 gtacagcacc ggcggtgttg ccgacggcag cagccgcgct gctcgcgatc tcctggatgc
     1681 tgtcggtcag ttggtcggag ccagtcgcga cggcgtatgc ctgttccgat acctcagtga
     1741 ctgcgctcgc caacatgtca gcgaaagcgg tgaacgactc ggcggtggtc agcagctcgg
     1801 tcgcgacctt cgtgaccacc tgaccgccct cgcgtacttg ggtccgatct gaccaactga
     1861 ccacgaatcc accggcgatc cggtcgacgg tcacatcggc gtgcgcgtga ccagacctgg
     1921 tcgtggcgga gacaagcacg gtgagcgggt aacgacgtcc ggcccctgaa tccgcaactg
     1981 caacccggac ggcgtcgagc agggcctcgc cgtgggcggc tcgcatccgt tcgtcgagtt
     2041 cgcgggacgc cgcgttctgg tacacgaccg tcgcctgctc atcgatgacg atgacgcaat
     2101 gcggcgcctt ctccaggatc tcgaagacgt cgaaggattc gagcgcagtg cggccgggag
     2161 acgacggcgc gcgattacgt ctaggtccca tcggtccgtc ctggtcgcag gtctgctggt
     2221 ggtcgggctg gtgtttggtc gggccggtgt ttggtcgggc tggtgtcgcc ccatcggcag
     2281 gcgcgcgcaa gcgctgagcc aaaagggtgt gccaacgggc ctgaggcacc gtgatcaact
     2341 cgcagcgagg ggcaagtcgg gggtggaggt cggcggcgga gggctgcctg accggtagcg
     2401 ggcgaacgct gggacggcca gcccgagcag cacgacgccg accacgcacg ccaatccgcc
     2461 gctcaccgcg ctgaataccg gactcgtgag cgccgccatg ccgcccgcct ccaggtcgcc
     2521 gagacgcgga cccccggaga ccactacgaa gttgacggcg ctcaaccgcc ccagcaactc
     2581 gtcggggacg ttctggagca ggatcgtcgc tcgaaagacg gcgctcacca tgtcggccgc
     2641 gcccgcgatc gcaagcaaga ccgcgccgag caacaacgag cggctcatgc tgaagccggc
     2701 aatcgaccct ccccacacgg cgaccgatgc gagtaccgcc agcccctgac ggcgaacgtg
     2761 agccagcggt cctgaaaacg cagcgccgat aagtccgccg atcgccggcg ccgcgtaaag
     2821 caagccgacc gtccgcgcgc cgccaccgaa gtgcgtggca gccagcgcgg gaaagaccgc
     2881 ccgcggcata ccgaagatca tcgcgttcaa gtcgacgatg aagatcatcg cgatcacccg
     2941 gtgcctccgg gcccatcgca gtccttcaac gaccgcagcg aatcctggag gagcgccacc
     3001 accctgcggc ggcatcgggc gcaacctcac gatcgcgtag accgcgatag tgaacgtcgc
     3061 cgcgtcggtc gcgtaggcgg ccttcagccc agcggccgca atgacgacgc cagcgagcag
     3121 cggacccacc gtgccgctca gctgatacga cagttggttc agcgccgtag cggccggaat
     3181 tcgagccggg ggcagcaatc gcggaatgaa ggtggagcga gccggctggt cgatggcaaa
     3241 aaggcatccc tgcaaggcca taacgacata cagcagccac agctgtcgca cgttcagagc
     3301 cgcctgcaac gcaaagacaa acgacagcag cgcgagtccg gagctggtga gcagcaccaa
     3361 ctttcgccgg tcgacagcgt cggcgagcga gccaccgagc agcccgaccg cgatcagcgg
     3421 aatcgcaacg gcgagcccga tcgcaccgac cgcaaaagag gaatgggtca tcgcgtagac
     3481 ctgcacgggc accgcgacat tcgtcatctg gttgcccatc gccgatagcg actgcgcgat
     3541 ccacagcctc cggtactccg gcgattcgcg cagcggcctc atgtcagcaa gcagccgctt
     3601 gcgttcggcg ctcagtccac cggaatggtt cgcgcttcgc gcgtgatcaa cgtcgtccgt
     3661 atcgtcagcg gtcagccgaa ccagccctgc cggcaatcgc acgctggatg agtccgaccg
     3721 ttgccatcgg atcggccacc tcgaccacca gccgctcgaa gtggtcgtgc tcgagttcga
     3781 tcacgacggc attgtcacca cggtgaacgt cccaaaagat cagctgaccg tgctgatgga
     3841 aggtgccggc cgtaatgacg ccgggcacgt tgctgccggg cccacgccaa cctttggact
     3901 cgtgcacgat cgcgggatcg aaggacgccc cacgcacgtg ttcgagcggg atgtctagct
     3961 ggctcctcat gctccacagc ttgtcgagcc cgtgcaccac aacc
//
LOCUS       DATCBJ010000268         2842 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_519241, whole genome shotgun sequence.
ACCESSION   DATCBJ010000268 DATCBJ010000000
VERSION     DATCBJ010000268.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2842)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2842)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2842
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_519241"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1620)
                     /locus_tag="VII50_05480"
     CDS             complement(<1..1620)
                     /locus_tag="VII50_05480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5050330.1"
                     /translation="MSTFDVLPSRRLIFEEARRAALAEEAARRPRHHARPDDGDPALL
                     TSRIGWSLVSLLTGLTGLQAWGGSTLVPGANLVSVAVILIGLWGGWRAWVGRDGLSVR
                     GQALVLVALVVGIGFYAAAVWMTQPGYGTDAAAFDQYAASLAMHGLNPYTHSMAPALT
                     QFHVPQIFHTYRLNGTEVDSLSYPSASFLFYMPGLIVGFHTQAANVIDVAFWALSGFM
                     LWRMLPARASWVSGLILSAAVYLSFMLGGLTDALFVPFVLVAVYRWDRYGVHLEDSAA
                     RWIGPIALGIACSVKQTPWFLVPFLLIGLWIEHRENGTDGARVVGRYATTAVGTFLFL
                     NAPFIVANPAAWARGTFLPLLQPTIPDGQGLVDLTIFNHLGGGRLALYTCAGVACFGL
                     ALLAFRYRYAALKRAWVPLVGVSFFLPTRSFGSYLFMLVPAAIVAGVTASSPPVGWRV
                     RHSFQRWIGLGLVVGGVVTIGLAVAAVTTPSPLSIRIVDTQSTGQLGTIDQATVQVTN
                     HTGRPERPHFTVQTQAHATTFWNAVDASGHAV"
     gene            complement(1617..2522)
                     /locus_tag="VII50_05485"
     CDS             complement(1617..2522)
                     /locus_tag="VII50_05485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5050331.1"
                     /translation="MSSLLVVFGRAARKRSRPATLRIARFALLIFAVIATISLFGDSA
                     AHAAGGGYGPGIGGPAAAPGGFSSIVTTHAFGVTGGTVSANVPGGEAELTVPDGAFSQ
                     PIQAIITAPDLSGINAALPQLGFSGYSAIAGVGISIDDANGKPITSLFVHPLTLTITG
                     SGIDAGDLLVEFTSLSTAATLQAAVFTTGSVTVSLAADPDFAVLAPSSAASATSSASP
                     PASGSSPPTIVEGEQFTKSPGGSIVDKTLFAILAVLIVAAGVLMLVLRRRPPDSFIPR
                     HSAASAPPKQRRAPSPTFTPKHARS"
BASE COUNT          588 a          902 c          937 g          415 t
ORIGIN      
        1 cactgcgtgt ccagatgcgt cgacagcgtt ccagaacgtc gtcgcgtgtg cctgcgtttg
       61 gacggtgaag tgtggccgct ctggtcgccc agtgtggttc gtcacctgaa ccgttgcttg
      121 atcgatggtg ccgagctgcc cggtcgactg cgtgtcgacg atacgaatcg acaagggcga
      181 cggcgtggtg acggccgcca cggcgaggcc gatagtcaca acgccaccca ccactagccc
      241 gagcccgatc cagcgctgaa aactgtgccg cacgcgccac ccgacgggcg gcgagcttgc
      301 agtcactccg gcaacgattg cggcgggaac gagcatgaac aggtatgacc cgaacgagcg
      361 ggtcggcaag aagaacgaca cgccgaccaa cggaacccac gcccgcttga gcgcggcgta
      421 gcggtaccgg aacgccaaca atgccaggcc gaaacaggcg acacccgcgc acgtgtacag
      481 agcaagtcgc ccaccgccga ggtggttgaa gatggtcagg tcgacgagcc cttgcccatc
      541 gggaatcgtc ggttgcaaca acggaaggaa cgttcctcgc gcccatgccg cggggttagc
      601 aacgatgaac ggcgcgttga ggaaaagaaa tgtgccaact gcggtcgtcg catagcgacc
      661 gacaacgcgc gcgccgtccg tgccattttc gcggtgttcg atccacaacc cgatcaagag
      721 aaacggtaca aggaaccacg gcgtctgttt cacgctgcac gcgatgccga gtgcaatcgg
      781 gccgatccac ctcgcggcgg aatcctcgag atggacgccg tagcgatccc atcggtacac
      841 ggcgaccagc acgaagggaa cgaaaagcgc atcggtgagg ccgccgagca tgaacgagag
      901 gtacacggca gcgctgagaa tgaggcccga gacccacgat gcacgcgccg gcagcatccg
      961 ccacaacatg aagcccgaca gcgcccagaa cgctacgtcg atgacgttcg ccgcttgcgt
     1021 atggaaaccg acaatgagcc cgggcatgta gaagaggaag gacgccgacg ggtacgagag
     1081 gctgtcgacc tcagtgccgt taaggcggta agtgtgaaag atctgcggaa cgtggaactg
     1141 ggtcagcgcc ggcgccatcg agtgtgtgta cggattcagt ccgtgcattg cgaggctcgc
     1201 cgcgtactgg tcgaacgctg ccgcgtcggt gccgtacccg ggctgcgtca tccagaccgc
     1261 ggccgcgtaa aagccgatgc cgacaaccag ggcgacgagc acaagtgctt gaccacgcac
     1321 actgaggccg tctcggccga cccacgctcg ccagcctccc cacaagccga tcaggatcac
     1381 cgcaacgctc acgagatttg cgcccggcac cagcgtcgat ccgccccacg cctgcaaccc
     1441 ggtgagcccg gtcagcaagc tgaccagcga ccaaccgatg cgcgatgtca gcagcgcggg
     1501 atctccgtcg tccggccgcg catggtgtcg tggtcgtcgc gctgcttcct cagccagggc
     1561 ggcgcggcgc gcctcctcga agatgagtcg gcgcgaagga aggacgtcga acgtgctcat
     1621 gatcgggcgt gcttgggtgt aaacgttggc gatggagcgc gccgctgctt ggggggcgcg
     1681 ctggccgcgg aatgcctagg gataaaggaa tccggaggtc gccggcgcag caccagcatc
     1741 agcacgccgg ccgcaacgat gagaaccgcc aggatggcga acaaggtctt gtccacgatg
     1801 gagccgccgg ggctcttcgt gaattgctcg ccttcgacga tggttggcgg gctgctaccg
     1861 gacgccgggg ggctggcgct cgaggttgca cttgcagcag acgacggcgc gagcacagcg
     1921 aagtcaggat ccgcggcgag actcacggtg acgcttccgg tcgtgaacac tgccgcctgc
     1981 aaagtggccg ccgtcgacag gctggtgaac tcgacgagca ggtcacccgc gtcgataccg
     2041 gaaccggtaa tcgtcagggt gagcgggtgg acaaacaagc tggtgatcgg cttgccgttg
     2101 gcatcgtcga tgctgatgcc gacgccggca attgctgagt agccgctgaa cccgagctgg
     2161 gggagcgccg cgttgatgcc acttaggtca ggtgcggtga tgatggcctg aatcggctgg
     2221 ctgaatgcac cgtccggaac agtgagctct gcctcgccgc ctggcacgtt ggcgctcacc
     2281 gtgccgccgg tcacgccgaa ggcgtgcgtc gtgacaatgc tgctgaagcc accaggggcg
     2341 gccgccgggc ccccgattcc cgggccatag ccaccgcccg ctgcgtgagc tgcggagtcg
     2401 ccgaacagac tgatggtcgc gatcaccgcg aagatcagca gcgcgaagcg cgcgatccgc
     2461 aaagtggcag gacgggatcg cttccgcgcg gctcgtccga agacgacgag cagactcgac
     2521 acgcttcccc cagcccccca ctgcacacca aagctcgaac ttcatggttg cgccgcaacg
     2581 gcaatcgcgc tgccaaagtc gaactagaga cccgaatggc ctaaccgaag cccgcgcgag
     2641 aaagcgggcg aatctgagtg agcacggttg acgcccgaaa acgtagctgc cctccgaaaa
     2701 tccgacggaa agcacagcga atactcgcaa gcaggcaccg aatgagtgcg ttggccggta
     2761 aggtagctcc tcgtgaccgc tgtggcgcag atgactggcg agcccggcgc tccagcgcga
     2821 ttcagccacg aagccctgct ct
//
LOCUS       DATCBJ010000269         4202 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_474202, whole genome shotgun sequence.
ACCESSION   DATCBJ010000269 DATCBJ010000000
VERSION     DATCBJ010000269.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 4202)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4202)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4202
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_474202"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(127..2721)
                     /gene="clpB"
                     /locus_tag="VII50_05490"
     CDS             complement(127..2721)
                     /gene="clpB"
                     /locus_tag="VII50_05490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HEY5050332.1"
                     /translation="MDTYKLTTKSQEAVADAIRRAVAQGNPHVEPVHLLLALIGQADG
                     TAAPLLEALGVSLSTLRGDADQITARLPRATGSTVSAPGMDRPSMQVISAAAKRATDL
                     GDEYISTEHLLVGLADEGGEVASLLRRHGATSEALLGAFESVRGHARVTSPDPESTFQ
                     ALEKYGVDLTARARGGELDPIIGRDTEIRRVVQVLARRTKNNPVLIGEPGVGKTAVVE
                     GLAQRIVAGDVPESLRGKRLIALDLGAMIAGAKYRGEFEERLKAVLNEIKSSDGQVIT
                     FIDELHTVVGAGAAEGAMDAGNMLKPMLARGELRMVGATTLDEYRERIEKDPALERRF
                     QPVLVGEPTVEDTIGILRGLKSRYEAHHKVQISDAALVAAATLSDRYITGRFLPDKAI
                     DLVDEAASRLRMEIDSSPVEIDELRRAVDRMKMEELALAHEDDVASRERLERLRAELA
                     DRQEQLSALTVRWDIEKAGLNRVGELKQQLDDARGQAERAQRDGDFETASRLMYADIP
                     TLERSLAEAGVSAETGAAGGRDAMVKEEVGPDDIAEVVGAWTGIPAGRLLEGETGKLL
                     RMESELGRRLIGQRQAVAAVSDAVRRARAGISDPDRPTGSFLFLGPTGVGKTELAKAL
                     ADFLFDDERAIVRIDMSEYSEKHSVSRLVGAPPGYVGYEEGGQLTEAVRRRPYCVVLL
                     DEVEKAHLEVFDVLLQVLDDGRLTDGQGRTVDFRNTILILTSNLGSMYLIDPALDDDA
                     RRDAVMSLVRATFKPEFLNRLDEVILFDPLGTDELTRIVDLQIARLQARLADRRLTLS
                     VTPAALEWLALTGFDPLYGARPLRRLVQSAIGDQLARGLLSGAIHDGETVLVDVAASK
                     DALDVHPA"
     gene            complement(2726..3163)
                     /locus_tag="VII50_05495"
     CDS             complement(2726..3163)
                     /locus_tag="VII50_05495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019524250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEY5050333.1"
                     /translation="MLEDGVPVFVISVAAQLSGLHPQTLRTYDKLGLVSPDRAPGRGR
                     RYSSRDISQLREVQRLSQEHGVNLAGIKRILELENQVVALKQRLGELAHELELVHSAA
                     ARRVAEAHASHRRDLVPLRQATVAVVWPSQRFGDGPDVPQFGQ"
     gene            complement(3167..>4202)
                     /locus_tag="VII50_05500"
     CDS             complement(3167..>4202)
                     /locus_tag="VII50_05500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DnaJ C-terminal domain-containing protein"
                     /protein_id="HEY5050334.1"
                     /translation="KSEEKFKEISEAYDVLSDAKTRKEYDEARSLFGSGGFRMPGSGG
                     AGAPGGFNFDLGDLLGNARAGGAGAAGGLGDILGGIFSGGRRAGATSQQPRRGADVET
                     EVTLDFEHAVHGETVALRMTTPHPCSACKGTGAKAGTTPRVCPTCSGTGVTSRGAGGF
                     AFSEPCRDCRGRGLIVDDPCPVCHGSGRAMSEHTMQVRIPAGVNDGQRIRLKGKGSPG
                     ERGGPAGDLIVLVHVRPHALFGRAADNLTITVPVTFPEAALGSEIKVPTLDGPPVTLK
                     LPAGTTNGRVLRVRGKGVTRRDGSKGDQLVTIEVAVPHKVSGKAKEALEAYREATSAD
                     NPRAHLLEGE"
BASE COUNT          666 a         1437 c         1355 g          744 t
ORIGIN      
        1 caactcaccg cagagcaggt cgaggaggcg ctgcggttcg ccgtcctggt atcagcggtt
       61 gcgtgtaccc gcgccggttc cgacccgccg tacctgactg acctgcccgc cctcagcaac
      121 tgacgcttag gcggggtgga cgtcgagcgc gtccttcgac gcggcgacgt cgaccagcac
      181 ggtctcgccg tcgtggatgg cccctgacag caacccgcgc gcgagttggt caccgatcgc
      241 cgactgaacc aaccgccgca aggggcgtgc gccgtagagt gggtcgaatc cggtcagcgc
      301 gagccactcc agcgccgcgg gcgtgaccga cagtgtgagc cgcctatcgg ctaaccgagc
      361 ctgtaggcgg gcgatctgca ggtcgacgat tcgggtgagc tcgtcggtgc caagcgggtc
      421 gaacaggatg acttcgtcta gacggttcag gaactcgggt ttgaatgtcg ctcgcactag
      481 cgacatcacc gcgtcgcgtc gtgcgtcatc gtccagtgcc gggtcgatga ggtacatcga
      541 gcccaggttc gacgtcagga tcaagatggt gttgcggaag tcgaccgtgc gaccctgacc
      601 atcggtgagc ctgccgtcat cgagcacctg gagcagcaca tcgaagacct caaggtgagc
      661 tttctcgact tcgtcgagca gcacgacgca gtacggacga cggcgaacgg cctccgtgag
      721 ttgcccgcct tcttcgtagc cgacgtatcc agggggtgcg ccgacgagtc gcgagactga
      781 gtgcttctcg gagtactcgc tcatgtcgat gcgtacgatc gcgcgctcat cgtcgaaaag
      841 gaagtcggcg agtgcctttg ccaactcggt ctttccgact ccggtcggac cgaggaacag
      901 gaacgacccg gtcggccggt cgggatcgga gatgccggcc ctggcacgac gcaccgcatc
      961 ggaaaccgct gctactgctt gcctttgtcc gatcaaacgg cggccgagct ccgactccat
     1021 tcgcaataac ttgccggtct ccccctcaag cagacggcct gcgggaatcc ctgtccatgc
     1081 gccgaccacc tccgcgatgt catcggggcc gacctcctcc tttaccatcg cgtcgcgccc
     1141 gcccgcagcg ccagtctcgg ctgatacgcc ggcttcggca agtgacctct cgagagtcgg
     1201 gatgtcggcg tacatcaatc gcgacgcggt ctcgaagtca ccgtcgcgtt gtgcacgctc
     1261 ggcctggccg cgcgcgtcat cgagctgctg cttcagttcg ccaacgcggt taagcccggc
     1321 cttctcgatg tcccatcgca cggtcagcgc actcagctgt tcttgacgat cggcgagttc
     1381 ggctcgcagc cgttcaaggc gctcacggct cgcaacgtcg tcctcgtgtg cgagcgcgag
     1441 ttcttccatt ttcatccgat cgaccgctcg ccgcagctcg tcgatctcga ctggcgatga
     1501 gtcgatctcc atgcgcaggc gcgaggctgc ctcgtctacg aggtcgatgg ccttgtcagg
     1561 aaggaagcgg ccggtgatgt agcggtcgga caacgtggcc gccgccacca acgcggcgtc
     1621 cgaaatctgc accttgtggt gcgcctcgta gcgcgacttc aacccacgca agatgccgat
     1681 cgtgtcttcg acagtgggct caccgaccag caccggctgg aagcgccgct ccagcgcggg
     1741 atccttctcg atgcgctcgc ggtattcgtc cagcgtcgtc gcaccaacca tgcgaagctc
     1801 gccgcgcgcc agcatcggtt tcagcatgtt gccggcatcc atggcgccct cagcagcgcc
     1861 cgcgccaacg acggtgtgca gctcgtcgat gaaggtgatg acctggccgt cggagctctt
     1921 gatctcgttg agcacggcct tcaggcgctc ttcgaattcg ccccggtact tcgcgcccgc
     1981 gatcatggcg ccgaggtcga gcgcgatcaa tcgcttgcca cgcaacgatt cagggacatc
     2041 gcccgcgacg atgcgttggg cgaggccctc gacgaccgcg gtcttgccga ctccgggttc
     2101 accgatcagc accgggttgt tcttcgtacg gcgtgcgagt acctgcacga cccggcggat
     2161 ctcggtatcg cggccgatga tcgggtcgag ctcgccgcca cgtgcgcggg cggtgagatc
     2221 gacgccgtac ttctccaatg cctggaacgt cgactcgggg tcgggcgagg tgacgcgcgc
     2281 atgtccgcga acagactcga acgcgccgag cagcgcttcc gaggttgcac cgtggcggcg
     2341 cagcaacgag gcgacctcgc caccttcgtc ggccaggccg acgaggagat gttcggtcga
     2401 gatgtactcg tcgccgagat cggttgcccg cttcgccgcc gcactgatga cttgcatgga
     2461 cgggcggtcc atgcccggcg ccgacaccgt cgacccggtc gcgcgaggca gccgcgcagt
     2521 gatctggtcg gcgtcgccgc gaagtgtgct gagcgacact cccaacgctt cgagcagtgg
     2581 cgcggcggtg ccgtcggcct gcccgatcag cgccagcagc agatgtaccg gctctacgtg
     2641 cggattgcct tgggcgacag cccgccgaat cgcgtcggca accgcctctt ggctcttggt
     2701 ggtcagcttg taggtgtcca tcggatcact gcccgaactg cggcacgtca ggtccatcgc
     2761 cgaagcgctg gcttggccag accacggcga ccgtcgcctg ccgcaggggg acaaggtcgc
     2821 gacggtgcga cgcgtgcgcc tcggccaccc ggcgggcagc agccgagtgg acgagctcga
     2881 gctcgtgagc caactcgcct agccgctgtt tcagcgcaac aacctgattc tccagctcga
     2941 ggatccgctt gatgccggcg aggttcacac cgtgctcctg cgacaaccgc tgcacctcac
     3001 gcaattggga gatgtcacgg ctggagtacc gccgcccacg acccggggca cggtcgggtg
     3061 agacaagtcc gagcttgtcg taggtgcgca gcgtctgcgg gtgcaggcca gacagttgag
     3121 cagccaccga gatcacgaac accgggacgc catcttcaag catcgtctac tcaccttcca
     3181 acagatgcgc tcgcgggttg tccgccgatg tcgcttcgcg gtaggcctcg agcgcttcct
     3241 ttgctttccc agaaaccttg tgtggcacgg cgacttcgat cgtgacgagc tggtcgccct
     3301 tgctgccgtc gcgacgcgtg acgcccttgc cgcgtacccg caagacgcgg ccgttcgtcg
     3361 tcccagccgg cagttttaga gtgaccggcg ggccgtcgag agtcggcacc ttgatctccg
     3421 agccgagcgc ggcctccggg aaggtcacgg gaacggtgat cgtcaggttg tcggcggcgc
     3481 ggccgaacaa cgcgtgcggt cgcacgtgca cgagcacgat gaggtcgccc gccgggccac
     3541 ctcgctcgcc aggagaaccc ttgcccttca ggcgaattcg ctgaccgtcg ttgacgccgg
     3601 ccgggatgcg cacctgcatg gtgtgctcac tcatggcccg gccgctgcca tgacagaccg
     3661 ggcatgggtc gtcgacgatc aggccgcggc cgcgacagtc gcggcacggc tctgagaacg
     3721 cgaacccacc cgcacctctc gacgtcacgc ctgtgccgct gcatgtcggg cagacccggg
     3781 gggtcgtgcc ggcctttgcg ccggtgcctt tgcacgcact gcatggatgt ggggtcgtca
     3841 tccgcagcgc gacggtctcg ccgtgcacgg cgtgctcgaa gtcgagggtc acttcggttt
     3901 cgacgtcggc gccgcgccgc ggctgctgcg aggtcgcacc cgcccggcgt ccgccgctga
     3961 agattccacc gaggatgtcg cccaggcccc ctgcggcgcc ggcgccacct gcgcgcgcgt
     4021 tgccgagcag gtcgcccagg tcgaagttga aaccgcccgg cgcaccggcg cctccgctgc
     4081 caggcatgcg gaatccaccg cttccgaaca gcgacctcgc ctcgtcgtac tccttgcgcg
     4141 tcttcgcatc cgagagcacg tcgtaggcct cggagatctc cttgaacttc tcctcggact
     4201 tc
//
LOCUS       DATCBJ010000270         2003 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_316378, whole genome shotgun sequence.
ACCESSION   DATCBJ010000270 DATCBJ010000000
VERSION     DATCBJ010000270.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2003)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2003)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2003
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_316378"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            340..1932
                     /gene="rny"
                     /locus_tag="VII50_05505"
     CDS             340..1932
                     /gene="rny"
                     /locus_tag="VII50_05505"
                     /inference="COORDINATES: protein motif:HMM:TIGR03319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HEY5050335.1"
                     /translation="MPTAVLVGLVIAMALVTVAASAAVVVVLRRSAASEATAAGALGL
                     DATYAEVAELRAQAATDAAETRRRAQEQADLVDDARRAYDAEVQVLKGELRESRSDLE
                     RREHRLADREARIDAEQRDVEVRERDLANLESEVTRRRAELEDVAEERRLILERTAGL
                     TAEAAKAELVTAMQNQAKREAAIVVRDIERAATEDGDRRARNIVTLAIQRVASEQTAE
                     SVVSVLHLPSDDMKGRIIGREGRNIRAFETVTGVNLIIDDTPEAVLLSCFDPVRREVG
                     RITLEKLVLDGRIHPHRIEEIYERSVAEVEQLCARAGEDALVEVGITSIHPELVALLG
                     QLRYRTSYGQNVLKHLIESAHLAGIMASELRLDQALLKRCALLHDIGKALTHEVQGSH
                     AIIGAEVARKYGENDDVVHAIEAHHNEVEVRTIEAVLTQAADAISGSRPGARRESLES
                     YVQRLERLEEIATSYEGVEKVFAMQAGREVRVMVLPDQVDDIQAQVIARDIAKQIEEE
                     LTYPGQIRVTVVRESRATELAH"
BASE COUNT          354 a          656 c          673 g          320 t
ORIGIN      
        1 gacccgccgg tgagcccgcg catagcctcg tgaatagtgg gaactgcccc cgcaaccgcg
       61 ggtttacgac atctgatatt cgcgacaaat ggtgctgacg gcacatcggg ctctcgggcc
      121 tttgacacga ctcatccacc cggcgcaagc cgtggcggtt tctttgtaag ggttcggcgg
      181 tagccggcgg tcgcaggtga tgccggtcgt ctaggcgtgg cgctcccatc ggaacgcgtc
      241 tacgcctggt ctggtgtttg tcgtcgtctt gccgttgtcg ctcggttctg cgtcggaggc
      301 agcttcgcag ctgcgccgac ctaggaagga gcccggcaca tgccaacggc ggtcctggtc
      361 gggctagtca ttgcgatggc gttggtaacg gttgcggcga gtgcggctgt ggtcgtcgtc
      421 cttcgccgat cggccgccag cgaggcaacg gcggcgggcg ccctcggtct tgacgcgacc
      481 tatgccgagg ttgccgagct ccgggcccag gcggcgaccg acgcggccga gacgcggcgc
      541 cgtgcccaag aacaggcgga cctggtcgat gacgcccggc gggcttatga cgctgaggtg
      601 caggtgctca aaggcgagct ccgggagtcg cggtccgacc tcgaacgccg cgagcaccgg
      661 cttgccgacc gcgaggcccg gatcgacgcc gaacagcgcg atgtcgaggt gcgtgagcgt
      721 gacctagcca acctcgaatc cgaggtcacc cggcggcgcg ccgagctgga ggacgtcgca
      781 gaggaacgcc ggctgatcct cgagcgcacc gccggtctga ccgccgaggc ggcgaaggcc
      841 gagctcgtca ccgcgatgca gaaccaggcc aagcgcgagg cagccatcgt cgtccgcgat
      901 atcgaacgcg ccgccaccga ggacggcgac cggcgagcgc gcaacatcgt gacgctcgcc
      961 attcagcgag tcgcgagcga gcagacagcc gaatccgtgg tttccgtcct gcatctgccg
     1021 agcgatgaca tgaaaggccg catcatcggc cgtgagggcc gcaatatccg ggcgttcgag
     1081 acggtgaccg gggtcaatct gatcatcgac gacacccctg aggccgttct gctctcctgc
     1141 ttcgatccgg tacgccgcga agtgggccgc atcaccctgg agaagctcgt tctcgacggc
     1201 cggatccacc cgcaccgcat cgaggagatc tacgagcgca gcgtcgccga ggtcgaacag
     1261 ctgtgtgccc gcgctggcga ggacgcgctc gtcgaagtcg gcatcacctc gatccatccg
     1321 gagctggtcg ccctgctcgg ccagctgcgc taccggacgt cgtacggcca gaacgtcctc
     1381 aagcacctga tcgaatcagc ccacctcgcc ggcatcatgg cgagcgaatt acgactcgac
     1441 caagcgctcc tcaaacggtg tgccctgctg cacgacatcg gtaaagccct cacgcacgag
     1501 gtgcagggca gtcacgcgat catcggcgca gaggtggccc gcaagtacgg cgagaacgac
     1561 gacgtcgtcc acgcgatcga ggctcaccac aacgaggtcg aggttcgtac catcgaggcg
     1621 gtactcaccc aggccgccga cgcgatcagt ggcagtcggc ccggcgctcg tcgggagtca
     1681 ctggagtcct acgtccagcg gctcgaacgg ctcgaggaga tcgcgacctc ctacgagggc
     1741 gtcgagaagg tgttcgcgat gcaggccggt cgcgaggtcc gcgtgatggt cttgcccgac
     1801 caggtcgacg atatccaggc gcaagtcatc gctcgtgaca tcgccaagca gatcgaggaa
     1861 gagctgacct atccaggcca gatcagggtg accgtggtcc gcgagtcgcg cgccaccgag
     1921 ctcgctcact agagacggag tccgcgtgag tgcactgaag gtcttgttca tcggtggcac
     1981 cggcatcatc agttcggcct gct
//
LOCUS       DATCBJ010000271         2019 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_179002, whole genome shotgun sequence.
ACCESSION   DATCBJ010000271 DATCBJ010000000
VERSION     DATCBJ010000271.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2019)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2019)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2019
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_179002"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..654)
                     /gene="dnaN"
                     /locus_tag="VII50_05510"
     CDS             complement(<1..654)
                     /gene="dnaN"
                     /locus_tag="VII50_05510"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011718842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HEY5050336.1"
                     /translation="MKFRVERDVFAEAVAWAARSLPVRPPVPVLAGLLLEVTDSPAGL
                     ALSSFDYEVSANAQIEVSAAQAGRALVSGRLLAEISRSLPAQPVDVSIEGTRVVVSCG
                     SARFTMPTLPVDDYPALPEMPATAGTIGADVFAAAVSQVAIAAGRDDTLPVLTGVRIE
                     IEDDRVTLAATDRYRLAVRELRWQPERAGLSIVALVPARTLADTAKALVGGAQVTVSL
                     "
     gene            complement(1039..>2019)
                     /gene="dnaA"
                     /locus_tag="VII50_05515"
     CDS             complement(1039..>2019)
                     /gene="dnaA"
                     /locus_tag="VII50_05515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003791382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HEY5050337.1"
                     /translation="SNRFAHAAAVAVAEAPAKAYNPLFIYGESGLGKTHLLHAIGHYA
                     MGLFHGARVRYVSSEEFTNDFINSIRDGKAEGFRRRYREVDVLLVDDIQFLENKEQTQ
                     EEFFHTFNTLHNANKQIVISSDRPPKQLVTLEDRLRNRFEWGLLTDVQPPELETRIAI
                     LRKKAAQERLNVPNDVMEYIASKIASNIRELEGALIRVTAFASLNRQGVDLSLAEVVL
                     KDLIPDSQGPEITAATIMGATASYFGISLEDLCGSSRSRVLVTARQVAMYLCRELTDL
                     SLPKIGQLFGGRDHTTVMHADRKIRSLMAERRSIYNQVTELTNRIKSQAK"
BASE COUNT          352 a          640 c          648 g          379 t
ORIGIN      
        1 cagggacacc gtgacctggg ccccgccgac gagcgccttc gcggtgtcag cgagcgtgcg
       61 cgccggcacc aaagccacga tcgacagacc ggcgcgctcc ggctgccagc gcagctcgcg
      121 cacggccagc cgatagcgat cggtcgcggc caacgtcacc cgatcgtcct cgatctcaat
      181 ccggacgccg gtcagcaccg gcaacgtgtc atcgcggccg gccgcgatcg caacctggga
      241 aacggccgcc gcaaagacat cggcgccgat cgtgccggcg gtcgccggca tttccgggag
      301 ggcggggtag tcgtcaaccg gcaacgtcgg catcgtgaac cgggcgctgc cgcacgacac
      361 gaccacccgg gtgccttcaa tcgacacgtc gactggctgc gcgggcaggc tccggctgat
      421 ctccgccaac aaccggcctg acaccaaggc ccggccagcc tgcgcagcac tgacttcgat
      481 ctgtgcgttg gcggagacct cgtagtcgaa gctggacaat gcgagcccgg ccggcgaatc
      541 ggtgacttca agcagcagtc cagcaagcac cggaacgggt ggccgaacag gcaagcttcg
      601 cgccgcccag gcgacagcct cggcaaagac atcacgctct acccggaatt tcatggtgcc
      661 ccccactgag ccaacctgtg cgggcccttc cttacggctt gtgtcattcc taatccttgt
      721 ctcaaggaag tcgtcatcgg tgcggtggac actgtggaaa agtgccgtcc gcgcaggtca
      781 ggtgctgaat tctgcggcgg cggcgttgtg gacgacagtg tcccgtcggt ggacagcctg
      841 gggactggcg cgatcgtagc gcggctcgcc cacacttcgt cccacgcgtc gggcggctcg
      901 cccaccggaa tcaccgaccg gtccacaagt ttgtccccag gctgtgcaca gtcgtcccag
      961 ccgaaacaca ggttgcctgg taccggccaa ttcgagacac cgtgaggtcg tcagaccggt
     1021 ggtcaccggg gtggtcatct atttggcctg cgacttgatg cggttggtga gttcggtgac
     1081 ctggttatag atcgatcggc gctcggccat caacgaccgg atctttcgat ccgcgtgcat
     1141 gaccgtcgtg tggtcgcggc cgccgaagag ttgaccgatc ttcggtaagg acaggtcggt
     1201 caactcccgg cacaggtaca tggccacctg cctggcggtc acgagtactc ggctgcgaga
     1261 ggagccgcag aggtcctcga gtgagatccc gaagtacgag gcggtcgctc ccatgatcgt
     1321 ggctgcggtg atttctggcc cttggctgtc gggaatgagg tctttcagga caacctctgc
     1381 cagggacagg tcgacgccct gccggttcaa gctggcgaac gcggtaaccc ggataagtgc
     1441 tccctcgagc tcgcgaatgt tgctcgcgat cttgctggcg atgtattcca tgacgtcgtt
     1501 tgggacgttc agccgctcct gcgccgcctt cttccggaga atcgcgatcc tggtctcaag
     1561 ttcgggcggt tggacgtcgg tcagcaggcc ccactcgaat cggttccgaa ggcgatcctc
     1621 gagcgtcact agttgcttcg gggggcggtc gctcgagatc acgatctgct tgttggcgtt
     1681 atgcaaggtg ttgaacgtgt ggaagaactc ttcctgcgtt tgctccttgt tctccaagaa
     1741 ctggatgtcg tcgacgagca gcacgtcgac ttcgcggtag cggcggcgga aaccctcggc
     1801 cttgccgtcc cggatcgagt tgatgaagtc gttggtgaac tcttcggagc tgacgtaccg
     1861 cactcgagca ccgtggaaaa gacccatggc gtagtgcccg atcgcgtgca gcaggtgtgt
     1921 cttgcccagc ccagactcgc cgtagatgaa caacgggttg taagccttgg ccggcgcttc
     1981 ggcgactgcc accgcggccg catgggcaaa tctgttgct
//
LOCUS       DATCBJ010000272         2360 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_189190, whole genome shotgun sequence.
ACCESSION   DATCBJ010000272 DATCBJ010000000
VERSION     DATCBJ010000272.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2360)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2360)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2360
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_189190"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2211)
                     /locus_tag="VII50_05520"
     CDS             complement(<1..2211)
                     /locus_tag="VII50_05520"
                     /inference="COORDINATES: protein motif:HMM:TIGR02669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIID/LytB domain-containing protein"
                     /protein_id="HEY5050338.1"
                     /translation="MLFAVTSAGERRSRPRARLQHWRTHRAGRAIASVAVAIASLAGV
                     TTALVVQAAPALADDFVPVPANGVFSITGHGFGHGIGMSQYGAFGAAQQGLAASQILS
                     FYYPGTTQQQVGDPPIRVHLTAYDSSGITMVAPPGQPMTVTDQATGTSANGPTAMYKV
                     QIDATAMHVLFLDPTDQTWKPFVVGATADTAGPVTFTTPAGVRMYSGDGTSRQYRGAI
                     RVVRLSPTAAAAVNVLDMQSYLYGVVPREMPDSWALPAALQSQAVAARSYALSVATPT
                     AAWDICDTTACQVYGGQAMVAANGTVTTIEGVNSSPAVDATNGVALYFQGAPAFAQFS
                     ASNGGQVAPGARPYLVAEADPYDTAAIDPNHDWTAQLTAAALQAAYPTIGTVQGLQVT
                     ARDGAGDFGGRIVAVQLVGSNATVNVNTYLGLRSSYWTAGGGPSQVPVILNRGAAIGH
                     LENASSAGPGILSVTGWSFNSISKEVPDFVDVYIDNALAGRLPANQSRPDVGAAYGNT
                     SPYHGFSGTFQTTGGTHTVCVKGIDGVTNPTLNCMSATLPSGNPFGNLESVTDIGTGV
                     QLTGWAIDPDTASPATVHVYVDGVWAGATTANAERDDVGNAYPGYSANHGFSIVVNAG
                     QGTHTICAYAINIGIGTTNPLLKCATITLNRNPRGGVSLAGVTDGVATVTGWALDPDT
                     TGPIAVHLYLDGVWAGMTTANLDGATQAAAVNPVYGTLHGFSISAKLGPGLHNLC"
BASE COUNT          357 a          777 c          819 g          407 t
ORIGIN      
        1 gcacagattg tgcaggccgg gcccgagttt cgcggagata ctgaagccgt gcagcgtgcc
       61 gtacaccggg ttcaccgctg cggcctgcgt tgcgccgtcc aggttcgcgg tcgtcatgcc
      121 cgcccacacg ccgtccaggt agaggtgcac cgcgatcgga ccggtggtgt ccggatcgag
      181 agcccagcca gtgaccgtgg ccacgccgtc cgtcacgcct gccaatgaga cgccgccgcg
      241 cgggttgcgg ttgagggtga tcgtcgcgca cttcaacagg ggattcgtcg taccgatgcc
      301 gatgttgatc gcgtacgcgc agatcgtgtg cgttccctgg cctgcgttca ccacgatgct
      361 gaagccgtga ttggcgctgt acccgggata cgcgttgccg acgtcgtcgc gttcggcgtt
      421 cgcggtcgtc gcgcctgccc atacaccgtc gacgtagacg tgcacagtgg ccgggcttgc
      481 cgtgtcaggg tcgatcgccc aaccggtcag ctgcacgcca gtgccgatgt cggtgacgga
      541 ctcgaggttg ccgaacgggt tgccgctcgg caacgtcgcg ctcatgcagt tcaacgtcgg
      601 gttcgtgacg ccgtcgatgc ccttgacgca caccgtgtgc gtgccaccag tggtctggaa
      661 cgtgccgcta aagccgtggt agggactcgt gttgccgtag gccgcgccga cgtcgggccg
      721 cgactggtta gccggcagcc ggcccgccaa ggcgttgtcg atgtagacgt cgacgaagtc
      781 gggcacttcc ttgctgatgc tgttgaacga ccagcccgtc acgctcaata ttcccgggcc
      841 tgcgctgctg gcgttctcga gatggccgat tgccgcaccc cggttgagga tcaccggtac
      901 ctggctcggg ccaccccccg ccgtccaata gctggatcga agtccgaggt aggtgttgac
      961 gttgacggtg gcgttactgc caacgagttg gacggcgaca atgcggccgc cgaagtcgcc
     1021 tgcgccgtcg cgtgcggtga cctggagtcc ttgcacggtg ccgatcgtcg ggtaagcggc
     1081 ttgcagcgct gctgcggtga gctgcgctgt ccagtcgtgg ttgggatcga tggccgccgt
     1141 gtcatacgga tcggcctcgg cgaccagata gggccgggca cccggcgcaa cctgcccccc
     1201 gttcgacgcc gagaactgcg cgaacgccgg cgctccttgg aaatacagcg cgacgccgtt
     1261 cgtagcatcg acggctggtg aggagttcac cccttcgatg gtggtgacgg tcccgttggc
     1321 cgcgaccatc gcctgaccgc cgtagacctg gcaggcagtc gtatcgcaga tatcccacgc
     1381 tgcggtcggc gttgcgaccg acaacgcgta cgaccgggcg gcgacggctt gcgactgcag
     1441 cgcggccggc aacgcccagc tgtcgggcat ctccctcggc accacgccgt acaggtacga
     1501 ctgcatgtcg agcacgttga ctgcggctgc cgctgtcggg ctcagacgca cgacgcggat
     1561 cgcaccgcgg tattggcgcg acgtgccgtc gccggagtac atgcgcacgc cagcaggcgt
     1621 cgtgaacgtg accggacctg cggtgtcggc ggtcgcgccg accacgaacg gcttccaagt
     1681 ctggtcggtc gggtcgagga acaggacgtg catcgcggtc gcgtcgatct ggaccttgta
     1741 catagcggtc gggccgttgg cgctcgtccc ggtcgcctgg tcggtgactg tcatcggttg
     1801 tccgggaggg gcgaccatcg tgatgccgct cgagtcgtat gccgtgaggt gcacgcgaat
     1861 cggcggatcg ccgacctgct gctgcgtcgt gcctgggtaa tagaagctga ggatctgcga
     1921 agcggccagg ccctgctgcg ccgctccgaa cgcgccgtac tggctcatcc cgatgccgtg
     1981 cccaaagccg tggccggtga tcgagaagac gccgttcgca ggtaccggca cgaagtcgtc
     2041 ggccaaagct ggcgccgcct gcacgacgag cgcggtcgtg accccggcga ggctggcgat
     2101 cgccaccgcg acgctcgcga tcgcgcgccc ggctcggtgc gtccgccagt gctgcaggcg
     2161 cgcccgcggg cgtgatcgac gctcacccgc agacgtaacg gcgaacagca cgcggcactc
     2221 ccggaggctc gtgggcacgg cgacggcgag ccccacaacg atgtcagcct tgtcacacta
     2281 accgcagagt tcccaatggc cccgcaggac acggccgttc cgcgggggtt aaggccagct
     2341 ggcggggcgc tggtcgtcaa
//
LOCUS       DATCBJ010000273         3505 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_397717, whole genome shotgun sequence.
ACCESSION   DATCBJ010000273 DATCBJ010000000
VERSION     DATCBJ010000273.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 3505)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3505)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3505
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_397717"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..290)
                     /locus_tag="VII50_05525"
     CDS             complement(<1..290)
                     /locus_tag="VII50_05525"
                     /inference="COORDINATES: protein motif:HMM:NF014662.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC family protein"
                     /protein_id="HEY5050339.1"
                     /translation="MKEILGDVDRWRRQGRRVALARVIDVEGSGPREPGATMAVCEDG
                     EVAGSVSGGCVEGAVMEAALGSLATGDRGLLSFGYSDADAFAVGLTCGGTVHL"
     gene            411..1289
                     /locus_tag="VII50_05530"
     CDS             411..1289
                     /locus_tag="VII50_05530"
                     /inference="COORDINATES: protein motif:HMM:NF013134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein subunit M"
                     /protein_id="HEY5050340.1"
                     /translation="MQVPAPFEYQRASTVKDAIGLMERLGSSARLVAGGHSLLPMMKL
                     RLANFEYLIDINDLRGELGYITVEPAQIRVGALTRHRELLESEALGATFPIFRDAERV
                     IADPVVRNRGTIGGSLCQADPSEDLSAVCTTLDASCVIQGSAGQRVVTMAEFYRGPYE
                     TAVGDGEILIEVRIPIKAGGSSAYEKVERRAGDWAVVSCGAAVWMSDGVITDARVGLA
                     AVGPNTTGIPAISEALRGKAPSEEVYAEAGAIASESCSPTTDLRGSIEYKRHLANELT
                     RRALRRAVARINGTEV"
     gene            1293..1811
                     /locus_tag="VII50_05535"
     CDS             1293..1811
                     /locus_tag="VII50_05535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009079242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HEY5050341.1"
                     /translation="MQVSMTVNGTPVTEEIEGRTLLVHFLRDQLQLTGTHWGCDTSNC
                     GTCVVWLDNEPVKSCTVLAAMAGGHDVRTVEGLEQDGVLDPVQQGFMECHGLQCGFCT
                     PGMMMTARALLDRNPDPSDGEIREAISGQICRCTGYTTIVRSIKWAAATEAAANATPE
                     AAGASVTAGSPA"
     gene            1808..>3505
                     /locus_tag="VII50_05540"
     CDS             1808..>3505
                     /locus_tag="VII50_05540"
                     /EC_number="1.2.5.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR02416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobic carbon-monoxide dehydrogenase large
                     subunit"
                     /protein_id="HEY5050342.1"
                     /translation="MTAVEDRPAGFVNNDEKPVGYGRMLRKEDPRFIRGMGHYVDDIQ
                     LPGMLHLAILRAPAAHARLVSIDTTAAAAHPKVKAVVTGAMLAEKGLAWMPTLSNDVQ
                     AVLVTDKARFQGQEVAFVVAEDRYAARDALELIEVEWEILTPVMDARQALAPGAPLIR
                     DDLEGKKDNHCFDWQTGDEAATEAVFAKADVVVSEDIVYPRVHPAPMETCGCVADYER
                     VAGKLTLWTTSQAPHAHRTVYALVSGLPEHKIRVIAPDIGGGFGNKVPIYPGYVCAIV
                     ASLVVGKPVKWMEDRSENLVSTGFARDYIMRGEIAATKEGKILAIRTNVLADHGAFNG
                     TAAPIKYPAGFFGVFTGSYDLEAAFCKMTAVYTNKAPGGVAYACSFRITEAVYLVERI
                     VDCLAYELKMDPAELRLKNFIKPEQFPYTTKTGWVYDSGNYEPAMRLAMEMAGYADLR
                     KEQAEKRARGELMGIGVAFFTEAVGAGPRKDMDILGLGMADGSELRIHPTGKAVLRVS
                     CMSQGQGHETTYAQIVAEEIGIPPADIQVVNGDTDNTPFGLGTYGSRSTPVSGAAAAL
                     "
BASE COUNT          669 a         1156 c         1125 g          555 t
ORIGIN      
        1 aggtgaactg tgccgccgca ggtcagcccg accgcgaacg catctgcatc ggagtacccg
       61 aaactgagga ggccgcgatc gccggtggcg agcgacccaa gcgccgcttc catgaccgcg
      121 ccctcgacgc agccacccga aacggagccc gcgacctcgc cgtcctcgca gacagccatc
      181 gtcgcgccgg gctcgcgcgg acccgaaccc tcgacgtcga tcactcgcgc caaggccacg
      241 cgacgaccct gccgccgcca tcggtcgacg tcgccgagga tctctttcac caccgcatcg
      301 tgcgcccgcg cttggcaggt gtccagcgac ctaagcattc ccttaggtgc cgttgacaac
      361 gttgccgtgg tgccgcggat actgccggga ggacgacgcg aggagtcgac atgcaggtac
      421 ctgcaccgtt cgagtaccag cgagccagca cggtcaaaga tgccatcggc ctgatggagc
      481 ggctgggcag ctcagcccgg ctggtggcgg gcggccacag cctcctgccg atgatgaagc
      541 tgcggctcgc gaacttcgaa tacctgatcg atatcaacga tctgcgcggc gagttgggct
      601 acatcacggt cgagcccgcg caaatccggg ttggtgcgct gactcgacat cgcgagctgc
      661 tcgagtccga agccttgggt gcgacctttc cgatctttcg cgatgccgaa cgcgtgatcg
      721 ccgacccggt cgttcgcaac agagggacga tcgggggatc gctgtgccaa gccgacccgt
      781 ccgaggacct gtccgcggtg tgtaccaccc tcgacgcgag ttgcgtcatc caaggcagtg
      841 cgggccagcg ggtcgtgacg atggccgagt tctatcgcgg cccgtacgag acggccgtcg
      901 gcgacggcga gatcctcatc gaagtccgca tcccgatcaa agccggtggc tctagcgcgt
      961 acgaaaaggt cgaacgtcgg gcaggtgact gggccgtcgt ctcctgcggc gccgcggtat
     1021 ggatgagcga cggcgtcatc accgacgccc gcgtgggcct ggcagccgtc gggccaaaca
     1081 ccaccgggat tccggcgatt tcagaggcat tacgaggcaa ggcgccgtcc gaagaggtgt
     1141 atgcggaggc gggcgcgatc gcatccgaaa gctgcagtcc gacgaccgac cttcgcggca
     1201 gcatcgaata caagcggcac ctcgcgaatg aactcacccg gcgggctctt cgccgtgccg
     1261 tggcccgcat caacggaaca gaggtctgag acatgcaagt gtcgatgacg gtcaacggca
     1321 cgccggtcac cgaggaaatc gaaggccgta cgctgctcgt ccacttcctt cgcgaccagc
     1381 tccagctcac gggtacgcac tggggctgcg acaccagcaa ctgcggcacg tgcgtggtat
     1441 ggctcgacaa cgagccagtg aaatcgtgca cggtgctcgc cgccatggcc ggcggccacg
     1501 acgtgcgaac ggtcgagggt ctcgaacaag acggcgtcct cgacccagtg cagcaagggt
     1561 tcatggagtg ccacggcttg cagtgcggct tctgcacccc cggcatgatg atgaccgcgc
     1621 gtgcgctgct cgatcgcaat cccgatccat cggacggcga gatccgcgag gccatctcgg
     1681 gccagatctg ccggtgtacc ggctacacca cgatcgtgcg atctatcaaa tgggcggctg
     1741 cgactgaggc tgccgcgaat gccacccccg aggccgccgg cgcctcggtc accgcaggga
     1801 gccccgcatg accgccgtcg aagatcgccc ggccgggttc gtcaacaacg acgagaagcc
     1861 tgtcggctat ggccgcatgc tgcgcaagga agacccgcgc ttcattcgcg gcatgggtca
     1921 ctacgtcgat gacatccagc tgcccggcat gctgcacctc gcgatcttgc gcgcgcccgc
     1981 ggcccacgcc cgcctggtca gtatcgacac gaccgccgcg gccgcgcacc cgaaggtgaa
     2041 ggccgttgtg accggcgcca tgctggccga aaagggcctc gcctggatgc cgacgctgtc
     2101 gaacgacgtg caggcagtgc tggtcaccga caaggccagg ttccagggtc aagaagtcgc
     2161 gttcgtcgtc gccgaagacc ggtacgccgc acgcgacgcg ctcgagctca tcgaagtcga
     2221 atgggagatc ctgacgccgg tgatggacgc caggcaggcg ctcgcacccg gcgcgccatt
     2281 gatccgcgat gacctcgagg gcaagaaaga caaccactgt ttcgactggc agaccggtga
     2341 cgaagcagcg accgaagctg tcttcgcgaa ggccgacgtc gttgtcagtg aagacatcgt
     2401 ctacccgcgt gtgcacccgg caccgatgga gacgtgcggc tgcgttgccg actacgagcg
     2461 cgttgcgggc aaactcaccc tctggacgac gagtcaggcc ccacatgccc accgcaccgt
     2521 ctacgcgctg gtgtcagggt tgcccgagca caagattcgc gtcatcgctc ccgacatcgg
     2581 tggcggcttc ggcaacaagg tgccgatcta ccccggctac gtgtgcgcga tcgtcgcatc
     2641 gcttgttgtc ggaaagccgg ttaagtggat ggaagaccgc tcggaaaacc tggtcagcac
     2701 cggctttgcg cgcgactaca tcatgcgtgg cgagatcgcc gcgaccaagg aaggcaagat
     2761 cctcgcgatc cgcaccaacg tgcttgccga ccacggggcc ttcaacggca ccgcagcacc
     2821 gatcaagtac ccagccggct tcttcggcgt tttcaccggc agctacgacc tcgaagcagc
     2881 gttttgcaag atgaccgcgg tgtataccaa caaggcaccg ggcggagttg cgtacgcatg
     2941 ctcgttccgc attaccgagg ccgtctacct cgtcgagcgc attgttgatt gccttgcgta
     3001 cgagctcaag atggaccccg cggagttgcg gctaaagaac ttcattaagc ccgagcaatt
     3061 cccctacaca accaagaccg gctgggtgta cgactcgggc aattacgagc cggccatgcg
     3121 gcttgcgatg gagatggcgg gatacgccga cctgcgcaaa gagcaagccg aaaagcgtgc
     3181 gcgcggtgag ctgatgggga tcggcgtcgc gttcttcacc gaggcggtag gtgccggccc
     3241 acgcaaggac atggatattc tcgggctcgg catggccgac ggttcggagc tgcggattca
     3301 ccccaccggc aaggcggtac tgcgcgtttc gtgcatgagc cagggccaag gccatgagac
     3361 cacatatgcg cagatcgtcg ccgaggagat cggcattcca ccggcagaca tccaggtcgt
     3421 caacggcgac accgacaaca cgccgttcgg tctcggaacc tatggcagcc gttcaacgcc
     3481 ggtgtcaggt gcggcggccg cgctc
//
LOCUS       DATCBJ010000274         2589 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_507097, whole genome shotgun sequence.
ACCESSION   DATCBJ010000274 DATCBJ010000000
VERSION     DATCBJ010000274.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2589)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2589)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2589
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_507097"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..587)
                     /locus_tag="VII50_05545"
     CDS             complement(<1..587)
                     /locus_tag="VII50_05545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009082728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEY5050343.1"
                     /translation="MSEVRRITHWIGGKPYTGESARHGEVFDPATGRVTGHVDFASSD
                     DVSQAVAAAADAWRDWRSASLSKRTSVMFAMRELLRQRSGDVAAAITSEHGKVLSDAA
                     GEVARGLEVVEFACGIPQLLKGGFSENVSTKVDVYSIRQPVGVVGVISPFNFPAMVPL
                     WFVPIAIACGNAVVLKPSEKDPSAANLLAELFAEAG"
     gene            complement(584..1924)
                     /locus_tag="VII50_05550"
     CDS             complement(584..1924)
                     /locus_tag="VII50_05550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009079007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HEY5050344.1"
                     /translation="MSNPTESAHAELVARHRYVMPSWMALYYDDPIEIASGVGRRVTD
                     GQGREYLDFFAGILTNMLGYNVPEVRDAIKAQADTGILHTSTLYLIRSQIELAEKIAK
                     LSGIPDAKVFFTSSGTEANEAALLLATQYRRSNQVLALRNSYHGRSFASVAITGNRGW
                     SASSLSPVNVSYVQSGYRYRSPFGHLSDADFIAACADDLRDVIITTTSGDVACMIAEP
                     IQGVGGFATPPDGMFAAFKEILDSYGILFISDEVQTGWGRTGEHFWGIQAHGVVPDAM
                     TFAKGLGNGTSIGGVVARAEVMDCLNANSISTFGGNPLVTAAANATLDYVLSHDLQAN
                     AAKVGTVLLEGIRGLQHTHQFIGDVRGKGLMIGVELVVPGSKTPDPAAATRMMEETRE
                     RGLLIGKGGLHGNVLRIAPALTITEAEAVEGLDVLAQSFAALAAANPSAAAIVR"
     gene            complement(1966..>2589)
                     /locus_tag="VII50_05555"
     CDS             complement(1966..>2589)
                     /locus_tag="VII50_05555"
                     /inference="COORDINATES: protein motif:HMM:NF027964.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase C-terminal
                     domain-containing protein"
                     /protein_id="HEY5050345.1"
                     /translation="ECLNPPVVWERNRGWYTTEPFSEPEVFDFPEGIGPVECVNVEHE
                     EVLLVPRWVEAERVTFKYGLGDEFIGVLKTLHKLGLDRVDKIKVGGVEVSPRDVVAAC
                     LPDPATLGSRMRGKTCAGTYVTGTGKDGRPRATYLYHVVDNEWSMREYGAQAVVWQTA
                     INPVVALELLATGAWKGAGVLGPEAFDAQPYLDLLVTYGSPWGMRDQ"
BASE COUNT          448 a          845 c          845 g          451 t
ORIGIN      
        1 cctgcctcgg caaagagctc ggcaagcagg ttcgccgccg acggatcctt ctcgctcggc
       61 ttcagcacta cagcgttgcc acaagcgatg gcgatcggga cgaaccacag cggcaccatc
      121 gccgggaagt taaacggtga gatcacgccg acgacaccga caggttgccg gatcgagtac
      181 acgtcgacct tcgtcgacac gttctcggag aagccgccct tcagcaactg tgggatgcca
      241 cacgcgaact cgacgacctc gagccctctg gcgacctcgc ccgcagcatc ggagagcacc
      301 ttgccgtgtt ctgaggtgat agccgcagct acgtcaccac tgcgctgacg cagcagctct
      361 cgcatcgcaa acatcaccga cgtgcgcttc gacagcgacg ccgatcgcca atcgcgccag
      421 gcgtcggctg cggccgcgac ggcttggctg acatcgtcgg acgacgcgaa gtcgacgtgc
      481 ccggtgactc ggccggtcgc cgggtcgaag acctcaccgt ggcgggccga ctccccggtg
      541 tacggcttgc cgcctatcca atgggtgatc cgacggacct cgctcatcgg acgatcgccg
      601 cggccgacgg gttcgcggct gccaatgcgg cgaaggattg cgccaaaacg tcgagccctt
      661 cgactgcctc agcctcagtg atggtcagcg cgggcgcgat gcgaagcacg ttgccgtgca
      721 ggccgccctt gccgatcagc agcccccgct cgcgggtctc ttccatcatt cgcgtggcag
      781 cggccgggtc gggcgtcttc gagcctggca cgacgagttc gacaccgatc atgagtcctt
      841 tgccgcgtac gtcgccgatg aactggtgcg tgtgttgaag gccgcggatg ccctcgagca
      901 agacggtgcc gaccttggct gcgtttgctt gcagatcgtg cgacagcacg tagtcgagag
      961 tcgcgttggc ggctgcggtc acgagcgggt tgccgccgaa ggtcgagatg gagttggcat
     1021 tcagacagtc catgacctca gcgcgtgcaa cgacgccacc aatcgacgtc ccgttgccga
     1081 ggcccttcgc gaaggtcatc gcatcgggca ccacgccgtg cgcctggatc ccccagaagt
     1141 gttcgccggt gcggccccag ccagtctgca cctcatcgga aatgaacaag atgccgtacg
     1201 agtcgaggat ttccttgaac gcagcgaaca tgccgtcagg cggggtcgcg aagccaccga
     1261 caccctgaat cggctcggcg atcatgcacg cgacgtcgcc tgacgtggtc gtgatgatga
     1321 cgtcacgcag gtcgtcggca cacgcagcaa tgaagtcggc atcagacagg tgcccgaacg
     1381 gactgcggta gcggtagccg ctctggacgt aactgacgtt gaccggcgag aggctcgacg
     1441 cgctccagcc acggttgccg gtgatggcga cgctggcaaa ggagcgtccg tggtagctgt
     1501 tgcgcaaggc cagcacctgg ttgctgcggc ggtactgcgt ggcgagcaac agggcagcct
     1561 cgttcgcctc cgtaccggag gaggtgaaga agaccttcgc gtcggggatg cccgacagtt
     1621 tcgcgatctt ctcggccaat tcgatctgcg accggattag atacagcgtc gaggtgtgca
     1681 agatgccggt gtcggcctgc gccttgatcg catcgcgcac ctcggggacg ttgtagccga
     1741 gcatgttggt gaggatcccg gcgaagaagt cgaggtactc ccggccctgc ccgtcggtga
     1801 cccggcggcc gacgcccgac gcgatctcga tcgggtcgtc gtagtagagg gccatccacg
     1861 acggcatcac gtagcggtgg cgcgcgacca gctcagcgtg cgcggattca gtggggttcg
     1921 acatctgtat cggtccttcg gtcggctggt tcgacagtac gtcttctatt ggtcgcgcat
     1981 tccccatggt gaaccgtagg tgacgagcaa gtcgaggtag ggctgcgcgt cgaaggcctc
     2041 cggccccagg acgcccgcac ccttccaggc gcccgtggcg agcaactcca gggcaacgac
     2101 gggattgatc gcggtctgcc agacgaccgc ctgcgccccg tattcgcgca tcgaccactc
     2161 gttgtcgacc acgtgataga ggtaggtggc tcggggcctg ccgtccttgc cggtcccggt
     2221 gacgtacgtg cccgcacagg tcttgccccg catccggcta cccagcgtgg ccgggtccgg
     2281 taggcaggcg gccaccacgt cgcgcgggga gacctcgacg ccgccgactt tgatcttgtc
     2341 gacgcggtcg agaccgagct tgtggagggt cttgaggacg ccaatgaatt cgtcgcccag
     2401 gccgtatttg aaggtgaccc gttccgcctc gacccagcgc gggacgagca gcacttcctc
     2461 gtgctcgacg ttcacacatt cgacgggccc gatcccctca gggaagtcga acacttcggg
     2521 ctcgctgaat ggctcggtcg tgtaccaccc gcggttccgc tcccacacca cgggcgggtt
     2581 gaggcactc
//
LOCUS       DATCBJ010000275         3565 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_381407, whole genome shotgun sequence.
ACCESSION   DATCBJ010000275 DATCBJ010000000
VERSION     DATCBJ010000275.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 3565)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3565)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3565
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_381407"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1173
                     /locus_tag="VII50_05560"
     CDS             <1..1173
                     /locus_tag="VII50_05560"
                     /inference="COORDINATES: protein motif:HMM:NF016047.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding repeat-containing protein"
                     /protein_id="HEY5050346.1"
                     /translation="ASSADQGQLSVYAAQRSTDGALTVQVINKTGGDLTSTLSVHNAS
                     TSAAQVFTYSAANLNAIVQGSDAEFSAGSVAHTYPANSITLLVIPTDIAVSRLGGANR
                     DATAAAVALAAFPTAGSAKAVVLARDDVYADGLTGSPLASALGGPLLMTNPAVLSADA
                     RSAITHSLSAGGTIYLLGGTSAISASTAATLTGLGYKLVRLGGADRYATASLIADQIA
                     ATHSVSHVYLATGLNFPDAESAADAAAVTNGVVLLTDGTAVAAETAQWETAHPGMTVV
                     AIGGSAAAADPSATPFVGADRYATAAKVAASVLPTPAGVVIATGANFPDGLAGAAYAA
                     HFGWAMVLVAPGSTTLDTDQASYLHAAASTVTTLVTVGQTVAVPPATVSAVLSGLG"
     gene            1216..1923
                     /locus_tag="VII50_05565"
     CDS             1216..1923
                     /locus_tag="VII50_05565"
                     /inference="COORDINATES: protein motif:HMM:NF016818.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0055072 - obsolete iron ion homeostasis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore-interacting protein"
                     /protein_id="HEY5050347.1"
                     /translation="MTELPAASTHLLSVADVVDVGPRMRRITIGGDALAEFVALPGQD
                     VVVHVSDGHGGGVSRRYTIRNLDTEVRRLDLDVVMHGDGPGAAWAASVAIGDGVEVFG
                     PRGKVLLAAAGWQLFVGDESAIPAIAEMIGALPVGTAAIALIEVTDADDEQSIRATGL
                     VDVRWLHRRATPPGESELLHQAVTSIEIPDVDRHAYLFGESRVVRRLRDDLGGRGLQP
                     HEISAKGYWNLGRVSGH"
     gene            complement(1930..2262)
                     /locus_tag="VII50_05570"
     CDS             complement(1930..2262)
                     /locus_tag="VII50_05570"
                     /inference="COORDINATES: protein motif:HMM:NF015735.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HEY5050348.1"
                     /translation="MQFLMFVCQDTAPVTPPPGPEGDVDEWVSTMEARGKRLAGDRLA
                     PERLAAAVRVRGGKVLVTDGPFLETKEILGGFDLLECRDMAEAIEVAAAHPFATRGVM
                     EIRPIWTG"
     gene            2388..3149
                     /locus_tag="VII50_05575"
     CDS             2388..3149
                     /locus_tag="VII50_05575"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEY5050349.1"
                     /translation="MSFDVGPDAYGRFMGRYSTPLAAAFVQLADVEPGTSALDVGCGA
                     GVLTAELVTLLGAGAVSAVDPSESFIAAVRSRFPDVDARHSSAEDLPYPDGSFECALA
                     SLVVSFMTDPVAGLAEMARVTRPGGLVGATVWDHAGTTGPLSTFWGAVLELDPDAHDE
                     SGLAGVREGQLAQLFDAAGLHDLHSTTLTVRVALADFVDWWEPFTLGVGPAGDYVARL
                     DEPERERLKARCAQLLPQSEPFEIAASAWTVFGRA"
     gene            complement(3166..3375)
                     /locus_tag="VII50_05580"
     CDS             complement(3166..3375)
                     /locus_tag="VII50_05580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5050350.1"
                     /translation="MTGPAAALIAFLVVVAIVVVVRFEVFCLRELARADDHQLLYLTR
                     RGWTVAILFSIPFGGMAYLLFGRPR"
BASE COUNT          556 a         1157 c         1210 g          642 t
ORIGIN      
        1 gcatccagcg ccgatcaagg gcagctctcg gtgtatgccg cgcagcgttc gaccgacggc
       61 gcgctcaccg tgcaggtcat caacaagact ggcggggatc tgacgagcac gctctcggtg
      121 cacaacgcat cgacgtccgc agcccaggta ttcacttaca gcgcagcaaa tctgaacgcc
      181 atagtgcagg gttcggacgc cgagttctca gcgggttccg tggcgcacac ctacccggcg
      241 aacagcatca cgttgctcgt tatcccgacc gacatcgcgg tgtcgcgact cgggggagcg
      301 aatcgcgacg cgactgcagc agcagttgca ctcgcggcgt ttccgaccgc aggctcggcg
      361 aaggccgttg tgctggcgcg cgacgatgtg tacgccgacg ggctgaccgg ttcgccgctg
      421 gctagcgccc tcggtggtcc gctgctgatg accaacccgg cggttctgtc cgctgatgcg
      481 cggtcggcga tcactcattc actgtctgcg ggcggcacta tctatctgct cggtggaacc
      541 agcgctatca gcgcatcgac tgcggcgacg ctcaccgggc tcgggtacaa gctcgtgcgc
      601 cttggcggcg ccgatcgcta cgcgacggcc agtctgatcg ccgatcagat cgcggccacc
      661 cacagcgtca gtcacgtcta tctggcaacc ggcctgaatt tcccggacgc cgaatcggct
      721 gccgatgccg ccgcggtcac caacggcgtc gtgctgctca ctgacgggac ggcggtcgcg
      781 gcggagacgg cgcaatggga gaccgcgcac ccgggcatga ccgtggtcgc gatcggggga
      841 tcggcggccg ccgctgatcc gtccgcgacg ccgttcgtcg gtgcggatcg ctacgcgacc
      901 gctgcgaagg ttgccgcgag cgtcttgccc acgcccgcgg gtgttgtcat cgcgaccggc
      961 gccaacttcc ccgacgggct cgccggagcc gcctacgcag cgcatttcgg ctgggcgatg
     1021 gtgctggtgg ccccgggttc gacgacgctg gacaccgatc aggccagcta tctgcacgcg
     1081 gccgcgagca cggtgacgac cctggtgacg gtcggtcaga cggttgcagt accgccggcc
     1141 acggtgagtg cggtgttgtc gggtctcggc tgatcggcat taggttggcc gtcccatcct
     1201 gggatgatca cgggtgtgac tgagctcccg gccgcgagta cccatctgct ttccgttgcc
     1261 gatgtggtcg acgtcgggcc acgcatgcgc cgcatcacta tcggcggcga cgcattggcg
     1321 gaattcgtgg cgttgcctgg gcaggacgtc gttgtgcacg tcagtgacgg tcacggcggt
     1381 ggggtcagtc gtcgctacac gatccgcaat ctcgatactg aggttcgtcg tctcgatctc
     1441 gacgtcgtga tgcacggtga cgggcctggt gcggcgtggg ccgcgtcagt cgcgattggt
     1501 gacggcgttg aggttttcgg cccgcgcggg aaggtgctgc tcgccgccgc cggatggcaa
     1561 ctgttcgtcg gcgacgagtc ggcgatccca gcgatcgcgg agatgattgg cgcgctgccc
     1621 gtcgggacgg cagccatcgc gctgatcgaa gtcaccgacg cagacgacga acagtcaatc
     1681 cgtgcaacgg gactggtcga cgtccggtgg ctgcatcggc gtgcaacccc gcctggagag
     1741 tccgagctgc tccatcaggc ggttacatca atcgagattc cagacgtcga ccggcatgcg
     1801 tatctgttcg gtgagagtcg cgtggtgcgc cgactccggg acgacctcgg gggccgcggc
     1861 ctacagccgc acgagatctc ggcgaagggc tattggaatc tgggccgcgt cagcggccat
     1921 tgacgcgcgt cagccggtcc agatcggccg gatctccatc actcctcgag tagcgaacgg
     1981 gtgggcggcg gcgacctcaa tcgcttcggc catgtcgcgg cattcgagca agtcgaagcc
     2041 gccgagaatc tccttcgtct ccaggaacgg gccgtccgtg accagcacct ttcccccgcg
     2101 cacgcggaca gcggctgcca ggcgttcggg agcgagccgg tcgccggcca atcgcttccc
     2161 gcgagcctcc atggtcgaga cccactcgtc aacgtcaccc tcgggtccgg gcggcggtgt
     2221 tacgggagcc gtgtcctggc atacgaacat caggaattgc atgacgtgta ctcctttgca
     2281 ggttgcctca tggtctcccg acgagcccgg atacccgaat tcgacgtttc gatcgagcac
     2341 ggtgaccaat gattggccgg caccggtggc gcgcctaggg tcagcggatg agctttgatg
     2401 tcggcccgga cgcctacggc cggttcatgg gccgctactc aacacccctg gctgccgcgt
     2461 tcgtgcagct cgccgacgta gagccgggca catccgcgtt ggacgtcggg tgtggcgccg
     2521 gagtcctcac tgcggagctc gtgacgcttc tgggagctgg ggcggtgtcg gcggtcgacc
     2581 cctcggagtc atttatcgcg gcggtgcgat cgcggtttcc agacgtcgat gctcggcact
     2641 caagcgccga agacctgccc taccccgacg gcagtttcga gtgcgcgctc gcttcgttgg
     2701 tggtgagttt catgaccgat ccggttgctg gtctcgctga gatggcgaga gtcacccgcc
     2761 cgggtggcct ggttggcgcc accgtgtggg accacgccgg gacgaccggt ccgctgtcaa
     2821 ctttttgggg cgcggtcctc gagctcgatc cggacgctca cgacgagtcg ggactcgccg
     2881 gtgtacggga gggtcagttg gcccagctgt tcgacgccgc cggcttgcat gacctccact
     2941 cgacgacctt gaccgttcgc gtggccctcg ccgacttcgt cgattggtgg gagcctttca
     3001 cgctcggagt gggtcctgcg ggggactatg tcgcccgtct cgacgagccc gagcgcgagc
     3061 ggttgaaggc tcggtgcgca cagttgctcc cgcagagcga gccgtttgag attgccgcgt
     3121 ccgcgtggac ggtgttcggt cgcgcgtagt gcgcgctgtt caggctcagc gtggcctgcc
     3181 gaacagcaga taggccatgc caccaaacgg aatcgagaag aggatcgcga cggtccagcc
     3241 tcgacgggtc aggtagagca gctggtggtc gtcagccctg gccagttctc gaaggcagaa
     3301 cacctcgaag cgaacaacca cgacgatcgc gacgaccacc agaaacgcga tgagcgctgc
     3361 ggccgggccg gtcatgctcc tattctccac cagccacgct gctagctagc tagctaggcg
     3421 gacgacgtct gctccggccc gttgcggacg gcggcacacc gacgcagaat gcaggcatgt
     3481 cacttgagcg cggtacaccc ccggatcctt tcgacgtcct gcccgcgaag ccgtggtttc
     3541 ggctggacag cgacgacatc gccga
//
LOCUS       DATCBJ010000276         8283 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_114027, whole genome shotgun sequence.
ACCESSION   DATCBJ010000276 DATCBJ010000000
VERSION     DATCBJ010000276.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 8283)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8283)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8283
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_114027"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..799
                     /locus_tag="VII50_05585"
     CDS             <1..799
                     /locus_tag="VII50_05585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEY5050351.1"
                     /translation="ELAADLFRQAQTYDPTFLLGHGADQLTTLDDGTLRVITDTGHTV
                     HAKAILVTAGIGIFTPRPLPAGQEYEGVGLRYFVPDPSALRDKRVLVVGGGDSAVDYA
                     LMLEQVCASVTVVHRRPEFRAHEASVEKMRASSVMIRTPYQVSGMQGDGRVEVVEITN
                     HDSGDVDRLEVDEVVAALGFIAELGPLMNWGMNLHQRHIVVDTTMATNVPRIFAAGDI
                     TEYPGKVRLISVGLGEAATAVNNAAVVIDPARKLFPGHSSDPAKQPG"
     gene            876..2087
                     /locus_tag="VII50_05590"
     CDS             876..2087
                     /locus_tag="VII50_05590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5050352.1"
                     /translation="MKGRIGLATPTMAIALGVLLVVLMVLGIPLSRASTSSSGGNLST
                     VFVILPFALVGVVVAYRQPRNAVGWILLAAGLFFIVDGDASLYTVLDYRQHGGHLLFG
                     PIAVLLQPSWAPAIVCMVLALLLFPDARLPLGRWRWILWPFVGVSALWVLGAFGIAAN
                     AIVTHSVHVTPSGDLISIDHPTGAAAWWGVVQNVFFPVVFLSGLGSIAKQIVSYRRSV
                     GERRQQLKWLLAGGTVFTVGGFATVVWSSASSPVEHTIGNLGTIGLAALPVFIGIAIL
                     KYRLFEIDRLISRTLSYAIVTGLLIGVYVGLIALTTRALPLSSPIGVAASTLAAAALF
                     TPLRRWVQRIVDRRFNRAHYDAEATVAAFSSGLRDAVDLDRVQEDLVAVVHLSMQPSH
                     VSVWLRERSPV"
     gene            2084..3298
                     /locus_tag="VII50_05595"
     CDS             2084..3298
                     /locus_tag="VII50_05595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5050353.1"
                     /translation="MTGRFSLARPATAASVGALVLALAVLSLPLAHATAGQSVAGAAG
                     DLVVVAPFALVGTIVAYRQSHNAVGWILLGFALFFVLDSDASIYTVLDYRQHHGRLFF
                     GPLAVLVQPSWAPAIVCMVLALLLFPDARLPLGRWRWILWPFLGISGLWVLGAFGIAA
                     GAIATHTVHIASSGNLTSLEHPTGGAAWWGVVEGVFFPAILVTGFASVARQVVSYRGS
                     TGERRLQLKWLLTGAAVFLVCGMALLVGSGPPSPVLQIVGNIGSVGIGVLPMCIGIAI
                     LKYRLFEIDRLISRTLSYAIVTGLLIGVYVGLIALTTRALPLSSPVGVAASTLAAAAL
                     FTPLRRWVQRIVDRRFNRAHYDAEATVAAFSSGLRDAVDLDRVQRDLVEVVRLSMQPS
                     HVSVWLRERSPI"
     gene            3433..3963
                     /locus_tag="VII50_05600"
     CDS             3433..3963
                     /locus_tag="VII50_05600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016572339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3145 domain-containing protein"
                     /protein_id="HEY5050354.1"
                     /translation="MSTRGVLYVHSATPAVTPHIEWAVAGVLSTPVRFEWLAQPASPG
                     AVRSEAPWIGEPGCGGRLSSALIGWPVRFEVTEDPSAGCDGERFAFTPSLGLYHAMTS
                     ANGDLVVPENRLRQLLTGQLLTSDVPIGKIEADAEGRGPLDSIRRLLGEPWDDELEPF
                     RYAGEGAPVRWLHQVV"
     gene            complement(3988..5388)
                     /locus_tag="VII50_05605"
     CDS             complement(3988..5388)
                     /locus_tag="VII50_05605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020730624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HEY5050355.1"
                     /translation="MTTTSTDTALDPRSPRARLTALFDEGTLETITADDDSGVLAGVG
                     RMLRTTAVAFACDPTIQGGAMGSAGCSAIVAAYDRALLDGVPIVGLWHSGGARLREGV
                     ESLDGVGRVFAAMTLASGKVPQISVVLGPAAGGAAYGPALTDIVIMGPAGRIFVTGPD
                     VVRSVTGEDVDMERLGGAEVHGRRSGVVHIVTDSEEDALERARALTDLLGNQGKIGDT
                     EIVDTNLESLLPEGAKRAYDVHPLINGVLDSPGVELHAKWAPNIVTVLGRLGGRTVGV
                     VANNPLRLGGCLDATSAEKAARFVRTCDAFGVPLVVVVDVPGYLPGLGQEWDGVVRRG
                     AKLLHAFAEAVVPRVTLITRKAYGGAYIAMNSRSLGATAVLAWPGAEVAVMGSVAAVR
                     ILHRRKLAEVGEALRPSLELELAAEHEKLAGSIDKAVAIGVVDRVVSPAETRSTIAEA
                     IASAPARRGSHGNIPL"
     gene            complement(5405..6646)
                     /gene="fabF"
                     /locus_tag="VII50_05610"
     CDS             complement(5405..6646)
                     /gene="fabF"
                     /locus_tag="VII50_05610"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HEY5050356.1"
                     /translation="MNATTQSKRVVVTGLGATTPIGGDVASTWAALLAGKSGVGVLTE
                     EWAAELPVRIAARIEVEPLDVLDRVEARTLDRSQQFAIIAAREAWAQAGKPDVEKERL
                     GVVFASGIGGVTTLLNQYDVLREKGPRRVSPHTVPMLMPNGPAAAVGLDLQARAGVHT
                     PVSACASGAEAIAYGLEMIRNGRADVVVCGGTEAAIHPLPLAAFAAMRAMSTRNDEPL
                     RASRPYDKGRDGFIMGEGAGAVVLESEEHAKARGATILGEIAGAGITSDGYHIAAPDP
                     EGRGVVRALRAALESANLSPSDVTHINAHATSTPAGDVAEAKAIREVFGSAADAVVVS
                     APKSTFGHLLGAAGAVEAIATLLSVTQHIVPPTLNLDDRDDDVDLDVVTVDPRPLREG
                     PAISNAFGFGGHNVVLVIQPA"
     gene            complement(6776..7030)
                     /locus_tag="VII50_05615"
     CDS             complement(6776..7030)
                     /locus_tag="VII50_05615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEY5050357.1"
                     /translation="MALSQQEILAGLAEIVNEVAGIPASDVQMDKSFTDDLDVDSLSM
                     VEVVVAAEEKFGVRIPDDDVKNLKTVGDAVTYIEKAGVSA"
     gene            complement(7103..8107)
                     /locus_tag="VII50_05620"
     CDS             complement(7103..8107)
                     /locus_tag="VII50_05620"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HEY5050358.1"
                     /translation="MKSQPGSSGARIVAIGEHRPTRIVTNEEICRNIDSTDAWIRERS
                     GIITRRYAAPDESVVDMATSAASKALAASGVAPEEVDLILLATCTHPYQTPGGSSEVQ
                     DRLGAINSGAMDINAACAGFAYALSVANDAVRGGSARHVVVIGSEKMTDFIDPHDRSM
                     AFLFGDGAGAAVVSASPTSGIGPVVWGSDGSHIKTITQEPTYTQVQHALATGESLPPV
                     ALKMAGQTVFRWAVTKMAPVAQKALDAAGVRVDELGAFVPHQANLRIVESLVKSLKLP
                     DTVAIGRDMETQGNTSSASIPLALSALLERGEVHSGQPALFIGFGAGLTYAAQVVEVP
                     "
     gene            complement(8104..>8283)
                     /locus_tag="VII50_05625"
     CDS             complement(8104..>8283)
                     /locus_tag="VII50_05625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-binding protein"
                     /protein_id="HEY5050359.1"
                     /translation="LPPGSLIGRVVTRREAQEVVASQGGVLVEWLVENGDPVSPGQPL
                     ARLHPDVLRPDLELT"
BASE COUNT         1229 a         2766 c         2787 g         1501 t
ORIGIN      
        1 cgagcttgca gctgacctgt tccggcaggc gcagacctat gacccgacgt tcttgctcgg
       61 tcatggcgcg gaccagctga cgacgctcga cgacggaacg cttcgcgtca tcaccgacac
      121 cgggcacacg gttcatgcca aggcgatcct ggtcaccgcc ggcatcggca tcttcacgcc
      181 gcggccgttg cccgccggcc aggagtacga aggcgtcggc ctccgctact tcgtccccga
      241 cccaagcgca ctgcgtgaca agagggtgct cgtcgtcggt ggcggcgaca gcgcggtcga
      301 ctacgcgctg atgctcgagc aggtgtgcgc ctcggtcacg gtcgtccacc ggcggcccga
      361 attccgtgcg cacgaggcat ctgtggagaa gatgcgggcc tcgtcggtaa tgatccgcac
      421 gccctatcag gtgagcggta tgcagggcga cggccgggtc gaggtggtcg agatcaccaa
      481 ccacgacagc ggggacgtcg accggctaga ggtcgacgaa gtcgtggcgg cgctcgggtt
      541 catcgccgaa ctcggcccgc tgatgaactg ggggatgaac ctgcatcagc ggcacatcgt
      601 ggtcgacacg acgatggcca ccaacgtgcc gcgcatcttc gcggccggcg acatcaccga
      661 gtacccaggc aaggtgcggc tgatctcggt cgggctcggc gaggcggcta ccgccgtcaa
      721 caacgcggcg gtcgtgatcg acccggcgcg caaactgttc cccggccatt cctccgaccc
      781 ggccaaacag ccgggctgac gggtcggccc ccagcccctc ggcgtcccct ggttcgtagc
      841 gttctcggaa cgatcggcgc ctaacctgtc cgtctatgaa gggcagaatc gggctcgcga
      901 cgcccacaat ggcgattgcg ctgggcgtgc tgcttgtcgt gctgatggtc ctcggaatcc
      961 cgcttagccg cgcctcgacg tcgtcgtcgg gcggcaacct gtcgacggtc ttcgtgatct
     1021 tgccgttcgc gttggtcggg gttgtggtgg cttaccggca gccgcgcaac gccgtggggt
     1081 ggatcctgct ggccgcgggg ttgttcttca tcgtcgatgg tgacgcttcg ctgtacaccg
     1141 tgctcgacta ccgccagcat ggcggacact tgctcttcgg gccgatcgct gtcctgctgc
     1201 agccttcgtg ggcgcccgcg atcgtctgca tggtgcttgc cctcctgctg ttccccgacg
     1261 cgcgactgcc gctggggcgc tggcggtgga ttctgtggcc gttcgtcggc gtctcggccc
     1321 tttgggtgct tggcgcattc gggatcgccg cgaacgcaat cgtgactcac agcgtgcatg
     1381 tcacgccgtc tggcgacctg atctcgattg atcacccgac cggcgccgcc gcgtggtggg
     1441 gtgttgtcca gaacgtgttc ttccccgtgg tctttctgag cggcttgggc tcgatcgcca
     1501 agcagatcgt gagttatcga cgatcggtcg gtgaacgacg gcagcagctc aagtggctgc
     1561 tcgccggcgg caccgtcttc accgtcggtg gcttcgctac tgtcgtctgg tcgagcgcgt
     1621 catcgcccgt cgagcacacc atcggcaacc tcggtaccat cggccttgca gcgctgccgg
     1681 tgttcatcgg catcgcgatc ttgaagtacc ggttgttcga gatcgaccgc ttgatcagcc
     1741 gcacgttgtc gtacgcgatc gttactgggc tgctgatcgg ggtgtacgtc ggcctcatcg
     1801 cgctgacaac gcgcgcgctg ccgctgtcgt cgccgatcgg cgttgccgcc tcgacgctgg
     1861 cggcggccgc gctgttcacg ccgttacggc ggtgggtgca acgcatcgtc gaccgtcgct
     1921 tcaaccgggc gcactacgac gccgaggcga ccgttgccgc cttcagttca ggtcttcgcg
     1981 acgccgtcga cctggatcgc gtgcaggagg acctggtggc cgtcgtccat ctgtcgatgc
     2041 agcccagcca cgtgtcggtc tggctgcgcg agcgcagccc cgtatgacgg gcagattctc
     2101 actcgccagg cccgcgacgg cggcgtctgt gggcgcgctc gtcctagcgc tggccgtcct
     2161 gtcgctgccg ttggcgcacg ccactgctgg tcaatcggtc gccggcgccg ctggagatct
     2221 cgtcgtcgtc gcgccattcg ccctggtcgg gaccatcgtc gcgtaccggc agtcgcacaa
     2281 cgccgtcggc tggatcctgc tggggtttgc cctgttcttc gtcctcgatt cggacgcgtc
     2341 gatctacacc gttctcgact accgccagca ccacggccgg ttattcttcg ggccgttggc
     2401 tgttctggtg cagccatcgt gggcgccggc gattgtctgc atggtgctag ccctcttgct
     2461 gttcccggat gcccgcttgc ccctcggacg gtggcggtgg atcttgtggc ccttcctggg
     2521 gatttcgggg ctgtgggtgt tgggcgcctt cggtatcgct gcaggcgcga tcgcgaccca
     2581 caccgtgcac atcgcgtcgt ccggcaactt gacatcgctg gagcacccga ctggcggcgc
     2641 cgcctggtgg ggggtcgtcg agggcgtgtt cttcccggcg attcttgtca cggggttcgc
     2701 ctccgttgcg cgacaggttg tcagctaccg cggatcgaca ggcgagcggc gccttcagtt
     2761 gaagtggctg ctgactggcg ccgcggtttt tctcgtctgc gggatggcgc tcttggtcgg
     2821 ctcgggaccc ccgtcgcccg tgctccagat cgtcggcaat atcggttccg taggcatcgg
     2881 tgtgttaccg atgtgcattg ggatcgcgat tctgaagtac cggttgttcg agatcgaccg
     2941 gttgatcagc cgcacgttgt cgtacgcgat cgtcactggg ctgttgatcg gggtgtacgt
     3001 cggcctcatc gcgctcacga cgcgggcgct gcccttgtcg tcgccggtcg gggtcgctgc
     3061 gtcgacgctg gcggccgcgg cactcttcac cccgttgcgt cggtgggtgc aacggatcgt
     3121 cgaccgtcgc ttcaaccggg cccactacga cgccgaggca accgttgccg ccttcagttc
     3181 gggacttcgc gacgccgtcg acctcgaccg ggtccagcgt gacctggtcg aggtcgtccg
     3241 cctctcgatg cagcccagcc acgtctcggt ctggctgcgc gaacgcagtc cgatctgacg
     3301 atcacccccg cgacgcgagg gtccggtatt ggcgcccggc cgcgcgctgg ggcagaatgt
     3361 gaccactggg acagctgatc agacgcgagg acgtcgggga aggcgcatct cgtccatctg
     3421 acggaggttc cggtgagtac tcgcggcgtc ctgtacgtgc acagcgccac gccggctgtc
     3481 acgccccaca tcgagtgggc ggtagccggc gtgttgtcga cgcctgtgcg cttcgaatgg
     3541 ctcgcgcagc ccgcatcgcc aggagcggta cgctccgagg cgccctggat cggcgaacca
     3601 ggttgtggtg gtcgactctc gtccgcgctg atcggttggc cggtgcgatt cgaggtcacc
     3661 gaagacccga gcgctggatg cgatggcgag cgcttcgcat tcacgcccag cctcggcctc
     3721 taccacgcca tgacgtcggc caacggcgat cttgtcgtcc ccgagaaccg gctacgtcag
     3781 ctgctgaccg gacagttgct gaccagcgat gtgccgatcg gcaaaatcga ggcggacgcc
     3841 gaggggcgcg gacctctcga cagcatcagg cgattgctcg gtgaaccttg ggacgacgaa
     3901 ctcgagccgt tccgctacgc cggtgagggc gctccggttc gctggctgca ccaggtcgtc
     3961 tagcaacagg tggtgtaacc gaaacagcta cagcgggatg ttgccgtgcg acccacggcg
     4021 cgcgggcgcc gacgcaatcg cttcggcaat cgtgctgcgg gtctccgccg gcgacacaac
     4081 gcggtcgacc acaccgatcg cgacggcttt gtcgatgctg cccgcgagct tctcgtgctc
     4141 ggcggcaagc tcgagctcga gtgagggccg cagcgcctcg ccgacctcgg ccagcttgcg
     4201 ccggtgcaga atccggaccg cggccaccga gcccatgaca gcgacctcgg ctcccggcca
     4261 cgcaagcacg gcagtggcgc cgagcgaccg cgagttcatc gcgatgtagg caccgccata
     4321 ggccttgcgg gtaatcaggg tcactcgggg cacgactgcc tccgcgaatg catgcaggag
     4381 tttcgcaccg cggcgcacca cgccgtccca ctcttgtccg agacctggca gatagccggg
     4441 gacgtcgacc accacgacca acggcacacc gaacgcgtcg caggtccgaa cgaaacgcgc
     4501 cgccttctcg gcgctcgtcg cgtcgaggca gccgccgaga cgcagcgggt tgttggcgac
     4561 gacaccgacg gtgcggccgc cgagacggcc cagcacagtg acgatgttcg gcgcccactt
     4621 cgcgtgtagc tcgacgccgg gggagtcgag gacgccattg atcagcgggt gcacgtcgta
     4681 ggcgcgtttc gcaccttcgg gcagtagcga ctcgaggttg gtgtcaacga tctcggtgtc
     4741 accgatcttg ccctgattgc cgagcaggtc cgtcagcgcg cgagcccttt cgagggcgtc
     4801 ttcttcgcta tcagtcacga tgtgcacgac gccacttcgg cggccatgca cctcggcgcc
     4861 gccgagacgc tccatgtcga cgtcctcgcc ggtgacgctg cgcacgacgt cggggccggt
     4921 gacgaagata cgaccggccg ggcccatgat gacaatgtcc gtgagtgccg gaccgtaggc
     4981 cgccccgcca gcggcgggcc cgagcacgac cgagatctgc ggcaccttgc ccgacgcgag
     5041 agtcatcgcg gcgaacaccc ggccgacccc gtcgaggctc tcgacgcctt cacgcagtcg
     5101 agcaccgccg gagtgccaca agccgacgat cgggacgccg tcgagcagcg cccgatcgta
     5161 ggcggcgacg atcgccgagc atcctgcgct gcccatggcg ccaccctgga tcgtcggatc
     5221 gcaggcgaag gcgaccgccg tggtcctgag catccggcca acgccggcca gcacgccgct
     5281 gtcgtcgtcc gcggtgatcg tttcgagggt gccctcgtcg aacaacgcgg tgagccgagc
     5341 acgaggtgag cgcgggtcga gcgcggtgtc ggtcgaagtc gtggtcacag gtcgtcgtct
     5401 cttctcaggc gggttggata acgagcacca cgttgtggcc gccgaagccg aacgcgttgg
     5461 agatggcagg accttcgcgc aacggccgcg gatcgacggt cacgacgtcg aggtcgacgt
     5521 cgtcgtcgcg gtcgtcgagg ttcagcgtgg gcggcacgat gtgctgggtc accgacagca
     5581 gggtggcgat cgcctcgacc gcgccggctg ccccgagcag gtggccgaac gtcgacttcg
     5641 gcgcgctgac cacgacggcg tcagccgcgg atccgaacac ctcgcgaatg gccttcgctt
     5701 cggcgacgtc gccggccggt gtcgaggtgg catgtgcgtt gatgtgcgtg acatcggacg
     5761 gcgagagatt ggccgactcg agcgcagcgc gcaacgccct gacgacacca cggccttcgg
     5821 ggtctggcgc ggcgatgtgg taaccgtcgg acgtgatgcc tgcgccggcg atctcgccga
     5881 ggatcgtcgc cccgcgtgcc ttcgcgtgct cctccgactc caacaccacc gcaccggcgc
     5941 cttcgcccat gatgaaaccg tcacggccct tgtcgtaagg gcgtgacgcg cgcaggggtt
     6001 cgtcgtttcg ggtcgacatc gcacgcatcg cagcgaacgc ggccagtggc agcggatgaa
     6061 tcgccgcttc ggtgccgccg cacaccacga cgtcggcgcg tccgttgcga atcatttcca
     6121 ggccgtacgc gatggcctca gctcccgacg cgcaggcgct gaccggggtg tggaccccgg
     6181 cccgggcctg caggtcaagt ccaactgcgg ccgcgggacc gttcggcatc agcatcggca
     6241 cggtgtgcgg tgagacgcga cgcggaccct tctcacgcag cacgtcgtac tggttcagca
     6301 aggtcgtgac gccaccgatg ccggaggcaa acaccacgcc gagacgttct ttctcgacgt
     6361 ccggcttgcc agcctgtgcc catgcctcgc gcgccgcgat gatggcgaac tgctgcgagc
     6421 ggtcgagcgt ccgtgcctcg acccggtcga gaacgtcgag gggttcgacc tcgatgcgcg
     6481 cggcaatgcg gaccgggagt tcagctgccc attcttcggt aagaaccccg accccggact
     6541 taccggccag cagtgccgcc cacgtactgg cgacgtcgcc gccgatcggc gtggtcgcac
     6601 cgagaccggt gacgacgacg cgtttgctct gggttgtggc gttcacgcct accgcatctc
     6661 ctcgattgtc atgcgctgtt tgtcaaagcg acgaagtcgt ccgtgtcatg gcgacgaacg
     6721 gcgccgcgcg atcccgatga gggagctgct cgccgtccgc ttcggttgcg actgttcagg
     6781 ctgagacgcc ggccttctcg atgtaagtga ccgcgtcgcc gaccgtcttc aggttcttga
     6841 cgtcgtcgtc cggaatccga acgccgaact tctcctcggc ggcgaccacg acttcgacca
     6901 tcgacagcga gtcgacgtcg aggtcatcgg tgaagctctt gtccatctgg acgtcggaag
     6961 ccgggatgcc agcaacctcg ttcacgatct ccgcgagacc ggcgaggatc tcctgctggg
     7021 acagggccat gggggtgctc cttctaggtg ggttgtattg gtggtcgaca ttgtgatcga
     7081 tatcgatacg cgaccgccgg ggtcagggga cctcgacgac ctgcgcagcg taggtgaggc
     7141 cggcgccgaa cccaatgaac agggcgggct ggccactgtg cacctcgccg cgctcgagca
     7201 gcgcggacaa ggccaacgga atcgaagcgg acgacgtgtt gccctgcgtt tccatgtcgc
     7261 ggccgatcgc gacagtgtcg ggcaacttca acgatttcac gagcgactcg acgatgcgca
     7321 ggttcgcctg gtgcggcacg aatgcgccga gttcatcaac tcggacgcca gccgcgtcga
     7381 gcgccttctg ggcgaccgga gccatctttg tgacggccca ccggaagacg gtctggccgg
     7441 ccatcttgag cgcaactggc ggcaaggact caccggtcgc gagtgcgtgc tggacctgcg
     7501 tgtaggtcgg ctcctgggtg atggtcttga tgtgcgatcc gtcgctgccc cacaccaccg
     7561 ggccgatgcc cgacgttggg ctcgcactca ccacggccgc gccggcgccg tcaccgaaga
     7621 ggaacgccat gctgcggtcg tgcgggtcga tgaagtcggt catcttctcc gaaccgatca
     7681 cgacgacatg ccgggcgctg ccgccgcgca cggcgtcgtt ggcgaccgaa agtgcgtagg
     7741 cgaaacctgc gcaggcggcg ttgatgtcca tcgccccgga gttgatcgcg ccgaggcggt
     7801 cttggacttc gctcgacccg cccggcgttt ggtacggatg cgtgcaggtg gccagcagga
     7861 tcagatcgac ctcttcgggc gccacacctg acgccgcgag tgccttgctc gccgcactcg
     7921 tcgccatgtc gacgaccgac tcgtctggcg ccgcatagcg acgggtgatg atgccgctgc
     7981 gctcgcgaat ccacgcatct gtcgagtcga tgttgcggca gatttcctcg ttggtcacga
     8041 tgcgagtggg ccggtgctcg ccgatcgcga cgatgcgggc ccccgatgaa ccgggttgtg
     8101 acttcatgtc aactccagat ccggacgcaa gacgtcgggg tgtaagcggg cgagcggttg
     8161 gccgggcgaa accgggtcgc cgttctcgac aagccattcg accagcacgc caccctgcga
     8221 cgccactacc tcttgcgctt cgcgcctcgt gacgactcgg ccgatcagcg agcccggcgg
     8281 gag
//
LOCUS       DATCBJ010000277         2852 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_447961, whole genome shotgun sequence.
ACCESSION   DATCBJ010000277 DATCBJ010000000
VERSION     DATCBJ010000277.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2852)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2852)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2852
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_447961"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1450)
                     /locus_tag="VII50_05630"
     CDS             complement(<1..1450)
                     /locus_tag="VII50_05630"
                     /inference="COORDINATES: protein motif:HMM:NF012926.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
                     /protein_id="HEY5050360.1"
                     /translation="MATDHRAHHRSRPHGPEQDDVDPAAAPVVLRDYAVLADGERGIV
                     VGPHGDFVWMCFPRWHDKALFSALIGGGGSYAITPMEPHVWGGYYEPGSLIWRSRWVT
                     NTAVIECREALAMPSRADRAVVLRRIVACQGTARIHCRLRTLADFGTGAVRAIHREGD
                     TWIVEHDHVQLRWMGAGTARVSRSTNSLEMEITVPEGGHHDLVMVVEPKGSVEEIPTA
                     DLAWRATESEWARRIPSLSSSAAPRDARHACAVLLGLTSGAGGMVAAATTSLPERARE
                     SNSYDYRYAWIRDQCYAGHAAFRAGIEELADCAVAFVSQRLLTDGPNLKPAYTVTGGR
                     VPDQTRLKLPGYPGGTDLVGNWVNNQFQLDAFGEALLLLATAAECDRLDVDGWRALGT
                     AASVVERRWREPDAGIWELDPHHWTHSRLTCAAGLRAAAKVASGSGATAASWTTLADT
                     IVAETGRTAVDDDGAWQRADDDPRVDAALLMAT"
     gene            complement(1454..2545)
                     /locus_tag="VII50_05635"
     CDS             complement(1454..2545)
                     /locus_tag="VII50_05635"
                     /inference="COORDINATES: protein motif:HMM:NF024771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HEY5050361.1"
                     /translation="MQSIDVRLYTIPTEEPEADGTLEWDSTSIVVVLPLLQNGIRGLG
                     YAVGDLSTATVARDALADVVIGLDVSDTAACWEAMVRRIRNLGRPGIASMAIAAVDIA
                     LWDTKARALDLPLHRLLGPIRDSVPIYGSGGFTTYTERDLCAQLAGWVEQGIPRVKMK
                     IGKDRGSSWEADLSRVRAARKAIGDDAELFVDANGAYGRKQALRLGNLYAEEFGVTWF
                     EEPVTSDDLDGLAALRDALPLEVTAGEYGYHLEYFRTMLASRSVDVLQADIGRCAGLT
                     EWLRIAATCAAYKTPYSAHCGPSIHVHAATVPPNLRHIEYFHDHVRADHLLFDGVLEP
                     HEGALRPADDRPGLGLTLKGKDAETFRVA"
     gene            complement(2560..>2852)
                     /locus_tag="VII50_05640"
     CDS             complement(2560..>2852)
                     /locus_tag="VII50_05640"
                     /inference="COORDINATES: protein motif:HMM:NF019499.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="vitamin K epoxide reductase family protein"
                     /protein_id="HEY5050362.1"
                     /translation="PDAALGIVSYASTLLLATAGGADRYRTTPWLSQLFAAKLLGDGL
                     VAVVLLREERRGGDGFCSWCLVASAAALLAAPLGLAEGIAATRVMRRARAKR"
BASE COUNT          496 a          944 c          929 g          483 t
ORIGIN      
        1 ctgtggccat gagcaatgct gcgtcgacac gcggatcgtc gtcggcgcgt tgccaggcgc
       61 catcgtcatc gacagccgtc cggcccgtct ccgccactat ggtgtcggcc aaggtcgtcc
      121 acgacgctgc ggtcgctccc gaccccgagg caaccttggc agcggcacgc agtcctgcgg
      181 cgcaggtgag ccggctgtga gtccagtgat gcgggtcaag ctcccagatg cccgcatccg
      241 gctctctcca gcgacgttcc accaccgaag cagcagtgcc cagcgctcgc caaccatcga
      301 cgtccaatcg gtcgcactcg gcagccgttg ccaggaggag tagcgcctcg ccgaaagcgt
      361 cgagttgaaa ctggttgttg acccagttgc ccacgaggtc ggtgcctcct ggatacccag
      421 gcagcttgag tcgtgtctga tcagggacac ggccgccggt gaccgtatac gccggtttga
      481 gattcggccc gtctgtgagg agccgctgac tgacaaacgc gacggcgcag tcagcgagtt
      541 cctcgatgcc ggcgcggaat gcggcgtgac ctgcgtagca ctggtcacgt atccacgcgt
      601 accgatagtc gtagctgttg ctctctcgag cgcgctccgg tagtgatgtg gtggcggccg
      661 caaccatgcc gccggcgccg ctggtgagcc ccagcagcac agcgcatgca tggcgcgcgt
      721 cacgtggcgc tgcagatgac gacagagagg ggattcggcg tgcccactcc gattcagtag
      781 ccctccaggc gagatcggcc gtggggatct cctcgacgct gcccttcggt tccaccacca
      841 tgacaaggtc gtggtggccg ccttccggca cggtgatctc catctccagc gaattcgtgc
      901 tgcgagagac ccgagccgtc ccggccccca tccagcgcag ttgcacgtgg tcgtgctcca
      961 cgatccaggt gtcgccctcg cggtggatcg cgcgtacggc tccggtgcca aagtcagcga
     1021 gcgtgcgcag tcgacagtga atgcgggcag tcccctggca cgcgacgatc cgccgcagta
     1081 ccaccgctcg gtcggcgcgg ctcggcatcg cgagggcctc gcggcactct atgacggcgg
     1141 tgttggtcac ccatcggctc cgccagatga gtgagccggg ttcgtagtag cctccccaca
     1201 cgtgcggttc catcggcgtg atggcgtacg agccgccgcc accgatcaga gcgctgaata
     1261 gcgctttgtc gtgccaacgc ggaaagcaca tccacacgaa gtcgccatga gggccgacta
     1321 cgatgccgcg ctcgccgtcc gccaggaccg cgtagtcgcg gaggacaacg ggagcagctg
     1381 caggatcgac gtcatcttgc tcaggcccat gtggccgcga acgatggtga gctcggtggt
     1441 ctgtcgccat cgatcaggcg acccggaagg tctcggcgtc cttgcccttg agagtgagtc
     1501 ccagaccggg tcggtcatcg gcgggtcgca aggcaccctc atgaggctcg agcacgccgt
     1561 cgaagaggag gtggtcggca cgcacgtggt cgtggaagta ctcgatatgt cgcaggttag
     1621 gtggcaccgt tgccgcgtgt acgtggatcg aggggccgca gtgcgcggaa tacggagtct
     1681 tgtaagccgc gcaggttgca gctatgcgga gccactctgt gaggccggcg caacggccaa
     1741 tgtccgcctg caatacgtcg accgaccggc tcgcgagcat ggtgcggaag tactcgaggt
     1801 ggtatccgta ttctccggcg gtgacttcga gaggaagcgc gtcacgcagt gcagccaagc
     1861 cgtcgaggtc atcggacgtt acaggctctt cgaaccacgt gacgccgaat tcctctgcgt
     1921 agagattccc cagccgaagc gcctgtttcc ggccgtaggc gccgttggcg tcaacgaaca
     1981 gctccgcatc gtcgccgatc gccttccgcg ccgcccgaac gcggctgagg tctgcctccc
     2041 acgaggagcc ccgatccttg ccgatcttca tcttgacgcg cgggatgccc tgctccaccc
     2101 acccggccaa ttgggcgcac agatcacgct cggtgtacgt cgtaaagccg ccgcttccgt
     2161 agatgggaac tgagtcgcgg ataggaccga ggaggcggtg gagcggcagg tcgagcgcgc
     2221 gggctttcgt atcccacaag gcaatgtcga cggcggcgat cgccatcgat gcgatccccg
     2281 gccgcccaag gttgcggatg cgccggacca tcgcctccca gcaggcggcc gtgtcactca
     2341 cgtccaaccc gatcacgacg tctgccagcg cgtcgcgagc cacagtggcg gtcgataaat
     2401 cgcccaccgc gtaaccgaga ccgcggatcc cgttctggag caggggcagt acgaccacga
     2461 tcgatgtgct gtcccattcc aaggttccgt ctgcctctgg ttcctcggtt gggatcgtat
     2521 agaggcgaac gtcgatcgat tgcacgcgca tctcatcgtt catcgcttcg ctctcgcgcg
     2581 gcgcatgacg cgggtcgcgg caatgccctc ggcaagtccg agcggtgcgg ccaacagagc
     2641 cgcggcggac gccaccaaac accaggaaca gaacccgtct ccgccgcgtc gctcctcgcg
     2701 cagcaggacg accgcgacga gaccgtcccc gagcagcttc gccgcaaaga gctgcgaaag
     2761 ccatggggtg gttcggtatc gatcagcacc accagcggtg gccaggagga gcgtcgacgc
     2821 atagctaacg atgccgagtg ccgcgtcagg ag
//
LOCUS       DATCBJ010000278         4830 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_511134, whole genome shotgun sequence.
ACCESSION   DATCBJ010000278 DATCBJ010000000
VERSION     DATCBJ010000278.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 4830)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4830)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4830
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_511134"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            333..521
                     /locus_tag="VII50_05645"
     CDS             333..521
                     /locus_tag="VII50_05645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5050363.1"
                     /translation="MSRSESPEVAAAASALEQEGEAVLIERVSSARRSGKEDEAAVGE
                     LLRRAAGENEAALDRLGR"
     gene            525..1340
                     /locus_tag="VII50_05650"
     CDS             525..1340
                     /locus_tag="VII50_05650"
                     /inference="COORDINATES: protein motif:HMM:NF014691.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HEY5050364.1"
                     /translation="MPFDDFVYPTLDDFVRAASVALQMDPSTVRNVVNVPLAESALGA
                     PAAGFGDHEEYPTLPMKVAVLLQRIAGNHALPDGNKRTALLCAILFSNLNGLAWRPPP
                     ADDPDGSETAEVVEAAAAGVLPFGALTAWVDHRLVFPAAPPEAGVEPAQAPPNAALVS
                     YAAEFIGHLPYENNTIRVGEHLVHEIHGYNPAAVYVRRLSGKTEGLSISEIIISVVGD
                     GYAQEELDAENAEAARYPLGPKEYWRGRMVGKYTYGADGHLMTDEDFEADWAE"
     gene            1875..2171
                     /locus_tag="VII50_05655"
     CDS             1875..2171
                     /locus_tag="VII50_05655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5050365.1"
                     /translation="MSSTRPPAQERVAAYYESCLRGLIEHVGIAMDQYRADGDAEQMN
                     TALYQYHRAARELWKFCWLDGGGSQLEFIASLIDASAPAVDWWQRGAPRRPKRG"
     gene            complement(2296..3063)
                     /locus_tag="VII50_05660"
     CDS             complement(2296..3063)
                     /locus_tag="VII50_05660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4259 domain-containing protein"
                     /protein_id="HEY5050366.1"
                     /translation="MGAWGPGIFSDDTASDIRGEYRELLEDQVPDGEATRRVIEAYGH
                     LDADEEHVLWLALAATQASLGRLDDEVRARAIDVIDSGRGLHLWDEAGPTELAKRKSV
                     LAKLRSQLTGPQATRKAVRRPWRHETDLRPGDILSFTASNGGMALLRVLRVDDQRVGV
                     APIVEWLDWGGRSLPRAKELERLKARAGNTPALGGPRRPATYRVARHRKKDQDWHESG
                     FVLAARATPRPEDEKTTAWTYLEWRGLSRDLEGQLAT"
     gene            complement(3269..3865)
                     /locus_tag="VII50_05665"
     CDS             complement(3269..3865)
                     /locus_tag="VII50_05665"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEY5050367.1"
                     /translation="MAIHPVVIDVHLPAGVAGPDPMDFDVRCFLVAHASGISLVDTGM
                     PGSASAIAEVLTSVGASWSDVTDVLLSHDHPDHIGSLDDVRARAPQAVVWGNAPVTAR
                     PLNEADVVRDLTVIATPGHTAGHVSLLDGSGALLVGDIVGNQNGSLSRAPAPFTADAV
                     EAERSLRKVAGIGFDRLLTGHGAELPDGTNALRELVKS"
     gene            4175..>4830
                     /locus_tag="VII50_05670"
     CDS             4175..>4830
                     /locus_tag="VII50_05670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5050368.1"
                     /translation="MLSPQERVDLVLASPTAAAMFVCAHAEGLTAENLADPATAMEVA
                     STAMNKLNPWSGQAVYERAAALDKVNGLRDLVAAVVGDTRNTWWWLDLQRERQLWVSD
                     ITLDPAAFVLPVPTKYTRWMIYAQHPNYADCIDTSTELDVAPAQVRSGLHNELAGAVV
                     TDWNPSYPLRQASITVSDTARVYEIHQPQAWRELALRYADLDEYAGPDANLLSSAGID
                     "
BASE COUNT          859 a         1607 c         1530 g          834 t
ORIGIN      
        1 cgcctcccac ccgacttcgc tgcccaaagc cgtcatctat tagacgcttg ggaccggctt
       61 cttgtagcgg agcactgtcc gtgggcgagc ctgaagccag caacgcaagc gacgtgctcc
      121 cctcgatacg tggcggatga acatccggat ttgccgcata gacgcgcgtt tggcgagaag
      181 aggatcaccc acataatgcc gcgatgagcg cgtgatgcgc cccgcgacca gcagaacgct
      241 tctcagccgg gcggtttccc tcgtcactga tgaggagtct cggggggagt cttgtcggtg
      301 tgtttgtcgg gaccgagtgc gagaatggcc ccatgtctcg atcggagtcc ccagaagtgg
      361 cggcagccgc ttccgcgctg gagcaggagg gcgaagcggt gctgatcgag cgcgtgtcgt
      421 ccgctcgaag gtcgggcaag gaagacgagg ctgcagtcgg cgagctgctg cgccgagcag
      481 ccggcgagaa cgaggccgcg ttggaccggc tcggtcgcta gcacgtgccg ttcgacgact
      541 tcgtctaccc aacgcttgac gacttcgtcc gggccgcctc ggttgccctg cagatggacc
      601 cgtccaccgt gcgcaacgtc gtcaacgtcc ctctcgctga atcagcgctg ggcgcaccgg
      661 cggcaggatt cggcgaccac gaggagtacc ccacgctccc catgaaggtt gctgtcctgc
      721 tgcagcggat tgccggcaac cacgcgttgc ctgacggcaa caagcgaacc gcgctgctct
      781 gcgcgatcct gttttcgaac ttgaacggtc tggcttggag gcctccgccc gccgatgacc
      841 cagacggcag tgagacggcc gaagtcgtag aggccgcggc ggccggagtt ctcccattcg
      901 gcgccctcac cgcgtgggta gatcaccgac tcgtcttccc agccgcacca ccggaggccg
      961 gcgttgagcc agcgcaggcg cccccgaacg ccgctctggt ctcgtacgcc gccgagttca
     1021 ttgggcacct gccttacgag aacaacacga tccgcgtggg agagcacctg gtccacgaga
     1081 tccatggcta caacccggcg gctgtctacg tgcgtcgact cagcggcaag accgaggggc
     1141 tgtctatctc ggagatcatc atctcggtcg tcggcgacgg ctacgcgcaa gaagagcttg
     1201 atgccgagaa tgccgaagca gcccggtacc cgttgggtcc gaaggagtat tggcgcggtc
     1261 gcatggtcgg gaagtacacg tacggcgctg acggtcacct gatgactgac gaggacttcg
     1321 aagccgattg ggcggagtag tcggctcgcg ccggcctcat gtcgtgcaag cagccatctc
     1381 tctgcgtcgc acgatggaac cggtcagcag acaggctctc cacggtgcat gtgaggtcgg
     1441 cggacgcccg ctccaggccg ccgatcgcgt tcaggtgaat gacctctcca tgtaatgacg
     1501 gtgattcagc cggtaaacgc ccgccatgat cgtccggcgt cgggttgggc caggctttgt
     1561 gggagtccgc gggggccggg tggcagtgat ggtgcgcttg tgcgtggccg caggcgatag
     1621 aacagcacgc ctggcttgcg tgccttgcgt tcaaacagtt tccggatact cctcagcgac
     1681 cgttcttcac ggcgacgagg aaaccaccga ccaccgtcgt tacatggaga ggtcattcag
     1741 gtgaacgcga tcaactgccg cgatgacgcg cgttcctgct cggcggggtc ggcgcggcaa
     1801 agcaattcga atagcacgga tgccatgtcc ggcggcggtg gcccggtctc gcgtgcatgg
     1861 cttagcgttg cggtgtgagc agtacccgcc cccccgcgca ggaacgtgtt gccgcgtact
     1921 acgaatcatg tctgcgtgga ctgatcgagc atgtcggcat cgccatggat cagtaccgcg
     1981 ccgatggtga tgccgaacag atgaacaccg ccctctatca gtaccaccgg gcggctcgtg
     2041 agttgtggaa gttctgctgg ttggacggtg gcgggtctca gttggagttc atcgccagtc
     2101 tgattgacgc ctcggcgcct gcagtggact ggtggcagcg cggcgcacca cgacgcccca
     2161 aacgcggctg atgacctcgg ctgaactagg tgagcgcgtg tcaactcgtc gtcgtagacc
     2221 gtgcgtcacg tccacgttcg aacgcgcgtc gttgcggcaa gacagtgcgt ttttacgtcg
     2281 gctccggtag tccggctagg ttgccaactg accttcgagg tcccgactga gtccgcgcca
     2341 ctccaggtac gtccaggccg tcgttttctc gtcctcggga cgtggggtcg cccgcgcggc
     2401 gaggacgaac ccactctcgt gccagtcctg gtccttcttc ctgtgacgag ccacccggta
     2461 cgtcgccggt cgccgcgggc cacccaacgc cggggtgttt ccggcgcgcg ccttcagtcg
     2521 ctctaactcc ttggcccgtg gcagcgatcg accaccccaa tccagccact cgacgatggg
     2581 cgccaccccg acacgctggt catccacccg aagcacgcgg agtagcgcca tcccaccgtt
     2641 gcttgctgtg aaggacaaga tgtcgcccgg ccgcaggtcg gtctcatgac gccacggtcg
     2701 tcgaaccgcc ttccgtgtcg cctgcgggcc agtgagctgg ctgcgcagct tcgccaacac
     2761 cgacttacgc ttggcaagct ccgtcggacc ggcctcgtcc cacaggtgga gaccacggcc
     2821 gctgtcgatg acgtcaatag cccgcgcacg cacctcatcg tcgagccgac cgagcgacgc
     2881 ctgagtagcc gccaatgcca accacaaaac atgctcttca tctgcatcca agtgcccgta
     2941 ggcctcgatg acccgacgcg tcgcctcgcc gtcgggcacc tggtcctcaa gcagctcgcg
     3001 gtactcacct cggatgtccg acgccgtgtc gtcagagaag atgcccggac cccatgcgcc
     3061 catggaagat attgtgccgc gcccagccgt tccaccagac ccgtatcgcc tcagcacggc
     3121 ccacaactct gcgcccacaa tcgaccgcca cagcggcaga agcggatgtt gcggcaactt
     3181 gtgcaaccga ggtcggcggt ggcgcgtcgt gattccggcc gatcacaacg agccccgaga
     3241 cgagcgtcag cgccagtgct gctgttgtct aggacttcac gagctcgcgt agcgcgttcg
     3301 tcccgtcggg aagctcggcg ccgtgcccgg tcaagaggcg atcgaagcca atccccgcaa
     3361 ccttccgtaa cgaacgctcg gcttcgacgg cgtccgcagt gaacggagct ggcgcccgcg
     3421 acagagaacc attctggttg ccgacgatgt cacccaccag cagggcgcca ctgccatcca
     3481 gcaagctgac gtgcccggct gtgtgtcccg gcgtcgcaat caccgtcaga tcgcgtacga
     3541 catcggcctc attcaacggt cgcgcggtca ccggtgcgtt cccccacacg acggcttgcg
     3601 gagcacgcgc ccgcacgtcg tccaggctgc cgatgtggtc ggggtggtcg tggctgagta
     3661 ggacgtcggt gacgtcgctc caagaggcgc cgacgctggt caagacctcg gcaatcgcgg
     3721 acgccgatcc gggcatgccg gtatcgacaa gggaaatccc ggaggcgtga gccacgagaa
     3781 agcagcgcac gtcgaaatcc atcgggtcgg gccccgcgac cccggccgga aggtgcacat
     3841 cgatgacgac ggggtgaata gccacggccg ctcctgcctg ctccgctgat ggcaccaata
     3901 atgcagccac tggcagccgt cgcatgctgc ggggcaacga cgcgaagtgc cccagcaacc
     3961 cgaccggcgt cgagaagtac cacaccgggc cagcggccgc caaaccgccg ccgcttcggg
     4021 ttctggcaga acaccgtgca cctcatcgga cggcaacttt gaccaccatg caaacctcgg
     4081 ccaaaggacc gtactcgcgg catgacagcg cgttggttga tgcgcccgct ttcaggtccg
     4141 caaagcggtt gacggagcgt tcgatactta gcgggtgctc agtccgcagg aacgcgtcga
     4201 tttggtgctc gcgtcgccga cggctgcggc aatgtttgtg tgtgcccacg ccgagggcct
     4261 gacggcagag aatcttgccg atccggccac tgcgatggaa gttgcgtcga ccgcgatgaa
     4321 caagctcaat ccgtggtccg gccaagctgt ctacgagcga gcagccgccc tcgacaaggt
     4381 gaacggtctg cgggacctgg ttgccgctgt tgtcggcgac accagaaaca cgtggtggtg
     4441 gctggacctg caacgcgagc gccagctgtg ggtgtccgat atcacgctgg accccgctgc
     4501 gttcgtgctg ccggtcccca ccaagtacac ccgttggatg atctacgccc agcaccccaa
     4561 ctatgccgac tgcatcgaca cctcgaccga acttgacgtt gcgcctgccc aggtccgctc
     4621 cggcttgcac aacgagctgg ccggcgctgt cgtcactgac tggaacccca gctatccact
     4681 gcggcaggcc agcatcacgg tgagcgacac cgcccgggtg tatgagatcc accagccgca
     4741 ggcctggcgg gagctcgcct tgaggtatgc cgatctcgac gagtacgcag gacccgacgc
     4801 caacctgctg tcatcggccg gaatcgacca
//
LOCUS       DATCBJ010000279         2099 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_423903, whole genome shotgun sequence.
ACCESSION   DATCBJ010000279 DATCBJ010000000
VERSION     DATCBJ010000279.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2099)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2099)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2099
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_423903"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..201)
                     /locus_tag="VII50_05675"
     CDS             complement(<1..201)
                     /locus_tag="VII50_05675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5050369.1"
                     /translation="MRGRSRQGILAALAAGTVAIGLPVAVALGHDGRGSGGGDDHGHG
                     HTSGIAHVLLISVDGMHQSDLAW"
     gene            complement(687..1898)
                     /locus_tag="VII50_05680"
     CDS             complement(687..1898)
                     /locus_tag="VII50_05680"
                     /inference="COORDINATES: protein motif:HMM:NF033540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS701 family transposase"
                     /protein_id="HEY5050370.1"
                     /translation="MKQLARVRGRLVEFAGEMFESMARKDQRRWGEVYLRGLMLDGKR
                     KSIEPMAGRLQDGDEQCLQQFVNQSPWDWTGVRERLAVRMVEEIEPAAWAIDDTGFPK
                     FGRESVGVARQYCGALGKIGNCQIGVSINAVSDEASCPIDWRLFIPAEWDHDSERRAR
                     CRLPEGERHRPKWAQAIEMLDELAGWGLRPAVIVADAGYGEITEFRQALEQRQLPYVV
                     QVKGVTSVLGVNAEPTLAAWKGRGRPPVARYDQKRSSLRELALAAGEGALSEIVWREG
                     SRGQLRSRFLALRVRPANVKLRRAAANNATELPVRWMLTEWPPGAQEPTKYWLADLPA
                     DTPLPQLVALAKIRWRIEHDYRELKDALGLDHFEGRSYPGWHHHVTLVAVAHAFLTLE
                     RTRRPQTRAAA"
BASE COUNT          372 a          738 c          661 g          328 t
ORIGIN      
        1 ccaggcaagg tccgactggt gcataccgtc gaccgaaatg aggagcacat gtgcgattcc
       61 gctcgtgtgt ccgtgcccgt gatcatcacc gccgcccgaa ccgcggccgt cgtgcccaag
      121 agccaccgcc accggaagac cgatcgcgac ggtaccggcg gcaagcgccg caagaatccc
      181 ctgcctggaa cgtccccgca tgtcgtcagc tccttgtcgc gtgagaccac cgttttacgg
      241 accggcaccc gggccgtggt tacgcacccg tgaacgtgtc gacgcacggc gatccatgag
      301 cgataccgtg aactcgatgt aacgcgcacg cggggcccga tcgcgcttgc ggcacgctgg
      361 gcgggctcag atcacgcggg cagccgcgca cgagcagtcg cgcgtgctag tggttggggc
      421 cctgtgaagg gagcggcgat atcgatctgg ccgacccgac cgacattgcc aacccgactg
      481 gtccggcgaa tcttcgcgat gtagcgggcg aacgcctctc cgtgctcgtc ttcgcgccga
      541 gcaacaggcc gctagtagtg ctccgttagg ttatggtggt ccgcggaggt aggtggtggt
      601 gggcggcagt tttcgtttgc aggtcgggca cgttccgttc cagcaggcga ggagccgttg
      661 gaggtcgtgc aggactgcga acagggtcag gctgccgccc tggtttgcgg gcgccgggtt
      721 cgctccaggg tgaggaaggc gtgtgcgacg gcgacgagtg tgacgtggtg gtgccagccg
      781 gggtagctgc ggccctcgaa gtggtcgagg ccgagcgcgt ctttgagttc acggtagtcg
      841 tgctcgatgc gccagcgaat cttcgcgagg gccacgagtt ggggcaaggg agtgtctgcg
      901 gggaggtcgg cgagccagta tttcgtcggc tcctgggcgc ccggcggcca ctcggtgagc
      961 atccagcgga ccggcagttc ggtggcgttg ttcgcggcgg cacggcgcag cttcacgttc
     1021 gcgggcctga cgcgcagagc gagaaagcgc gagcgcagct gaccgcggct gccttcgcgc
     1081 cagacgatct cgctcagcgc tccttcgccg gcggcgagcg cgagctcgcg cagcgaggaa
     1141 cgcttctggt catagcgcgc cacgggcggg cgtccgcggc ccttccacgc tgccagcgtc
     1201 ggctcggcgt taacgcccag gacactggtg acacccttga cctggacgac atagggcagc
     1261 tggcgctgct ccagcgcttg gcgaaactcg gtgatctcgc catagcccgc gtcggccacg
     1321 atcaccgcag gccgcagccc ccatcccgcc agctcgtcga gcatctcgat cgcctgcgcc
     1381 catttcggcc ggtgacgctc accctcgggc agccggcagc gcgcgcgccg ctcgctgtcg
     1441 tggtcccact cggccgggat gaacaaccgc cagtcgatcg gacacgacgc ctcgtcgctg
     1501 accgcgttga tgctcacccc gatctggcag ttgccgatct tgcccagcgc accgcaatac
     1561 tggcgtgcaa ccccgaccga ctcgcggccg aacttcggaa agcccgtgtc atcgatcgcc
     1621 cacgccgccg gctcgatctc ctcgaccatc cggaccgcca gacgctcgcg aaccccggtc
     1681 cagtcccacg gcgactgatt cacgaactgc tgcaagcact gctcatcacc atcctggagc
     1741 cggccagcca tcggctcgat cgacttgcgc ttgccatcca gcatcaaccc gcgcagatac
     1801 acctcgcccc aacgacgctg atccttccgc gccatcgact cgaacatctc accggcgaac
     1861 tccaccaacc gcccacgcac acgcgccaac tgcttcaccg acagcccaga cacagaacgc
     1921 atcctgacct ccctcgacga cggcaacgcc accaccgaga tacgcccccc accaacaaac
     1981 tcctgcaaca cctaacggag cactactagg ggtgcaccag ctgcacccaa accgttgtta
     2041 accgaaactg aaccgaaaga aacgcgcaac gagaagaaga agaacacccg gccggcgtg
//
LOCUS       DATCBJ010000280         2208 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_443705, whole genome shotgun sequence.
ACCESSION   DATCBJ010000280 DATCBJ010000000
VERSION     DATCBJ010000280.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2208)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2208)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2208
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_443705"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..644
                     /locus_tag="VII50_05685"
     CDS             <1..644
                     /locus_tag="VII50_05685"
                     /inference="COORDINATES: protein motif:HMM:NF042649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HEY5050371.1"
                     /translation="EVSVADLEWEKGSFHVKGDPSKSVTIQAIAMRAHGAGDLPEGVE
                     GGLEAQICYNPSNLTYPFGAYICVVDIDPGTAQVKVRSFVAVDDCGTRINPMIIEGQI
                     HGGLTDGVGMALMEMIAFDEDGNCLSSSLMDYLIPTALEVPEWQTGHTVTPSPHHPIG
                     AKGVGESATVGSPPAIVNAIVDALKPFNIRHADMPLTPSRVWETMQGRHRPPV"
     gene            641..1549
                     /locus_tag="VII50_05690"
     CDS             641..1549
                     /locus_tag="VII50_05690"
                     /inference="COORDINATES: protein motif:HMM:NF024869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC family protein"
                     /protein_id="HEY5050372.1"
                     /translation="MTIQPRMRQRLADRAAELAALRQPFVRATVVRAQSPTSARPGDD
                     AIILSDGSIEGFVGGHCAKESVRAAAFATLRDGEAMLLRILPDDSTVFPETPGAHLTI
                     NPCLSGGALEIFLEPHLPPAVVEVIGDTPVAEAVAALAEAVGFAVGRALPGQAPDGAI
                     AVIVSTHGHDEPECVRAALDAGVGFIGLVASRVRGESVLDAMDLTPSERARVHTPVGL
                     EIGAKTAAEIGLSIVAGIVSAVRLEGIAAPDAEPIEAPATAVDPICGMTVVVLPTTAH
                     LRVDGVDHWFCSQGCRDRYAAEVGQA"
     gene            1553..2143
                     /locus_tag="VII50_05695"
     CDS             1553..2143
                     /locus_tag="VII50_05695"
                     /inference="COORDINATES: protein motif:HMM:NF024214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HEY5050373.1"
                     /translation="MIVTGLVLAAGSSSRLGQPKQLLPFRGTTLLGATLDVARQCNFD
                     QLLVTVGGAAADVQVAVDFGDAEVVDNPDFSTGCGSSISAAMARLNTASEGIVLLLGD
                     QPGVSADTVRRLIDEGGTSQVAVCRYADGREHPFWFARSVFDELRTLHGDKAVWKVLE
                     SGRHSVAEVAIQSMAPIDVDTWADYEALLAASGVSP"
BASE COUNT          370 a          748 c          717 g          373 t
ORIGIN      
        1 tcgaggtatc cgttgccgac ctcgaatggg agaagggctc attccacgtc aagggcgacc
       61 cgtcgaagtc ggtgacgatc caagccatcg cgatgcgggc gcacggtgcg ggcgaccttc
      121 ccgagggcgt agaaggcggg ctcgaggcgc agatctgcta caacccctcc aacctgacgt
      181 acccgttcgg cgcctatatc tgcgtcgtcg acatcgatcc cggcactgcg caggtgaagg
      241 tgcgcagctt cgtggccgtc gatgactgcg gcacacggat caaccccatg atcatcgagg
      301 gccaaataca cggcggcctc accgacggcg tcggcatggc tctgatggaa atgatcgcgt
      361 tcgacgagga tggcaactgc ctctcgagtt cgctgatgga ctatctcatc cccaccgcgc
      421 tcgaggtgcc cgagtggcag accggccaca cggtgacacc gtcgccacat cacccgatcg
      481 gcgccaaggg cgtgggcgaa tctgcgactg tcggctcacc cccggcgatc gtcaacgcga
      541 tcgtcgatgc gctgaagccg ttcaacattc ggcacgccga tatgccgctg accccctcgc
      601 gggtatggga aaccatgcag ggaaggcatc ggccgccggt gtgacgatcc aaccacgcat
      661 gcgtcaacgc ctggccgacc gcgcggcaga gcttgccgcc ctccggcagc cgttcgtgcg
      721 tgcaacagtg gtgcgcgctc agtcaccgac gtccgcccga ccaggtgacg acgccatcat
      781 cctcagcgac gggtcgatcg agggtttcgt cggcggccac tgcgcaaagg agtcggtgcg
      841 cgcagcggcg ttcgcgacgc tgcgcgacgg cgaggccatg ctgctacgga tactcccgga
      901 cgacagcacc gtcttccccg agaccccagg cgcgcatctg accatcaacc cgtgtctgtc
      961 aggaggggcg ttggagatct tcctcgagcc gcaccttccg ccggcggtgg tcgaagtcat
     1021 cggcgacacc ccggtcgcgg aggccgtcgc cgcgctcgcc gaagccgtcg ggttcgcggt
     1081 cggccgcgcg ctcccgggcc aggctccgga cggcgcgatc gccgtcatcg tctcgacgca
     1141 cgggcatgac gaaccggagt gcgtccgcgc cgccttggat gcgggtgtcg gtttcatcgg
     1201 actcgtcgcg agtcgcgtcc gcggtgagtc cgtgctcgat gcaatggatc tcacgccgtc
     1261 cgaacgtgcg cgcgtccaca cgccggtcgg acttgagatc ggtgccaaga cggccgccga
     1321 aatcgggctc tcgatcgtgg ccggcatcgt gtcagctgtt cgccttgaag gcatcgccgc
     1381 tcccgatgcc gaaccgatcg aagcgcccgc gaccgccgtc gacccgatct gcggcatgac
     1441 cgttgtggta ctgccgacga cggcccacct acgcgtcgac ggtgttgacc actggttctg
     1501 tagccagggc tgccgggatc gttacgcagc cgaggtcgga caggcctgag tcatgatcgt
     1561 caccggactc gttctagcgg ccggctcctc cagccgactc ggccaaccaa agcagctact
     1621 cccctttcgc ggcaccacac tgctcggtgc gacgctcgac gtcgcacgcc aatgcaactt
     1681 cgaccagcta ctggtcaccg taggtggcgc ggctgccgac gtgcaggtcg cggtggactt
     1741 cggtgacgcc gaggtcgttg acaaccccga tttcagcacg ggctgcggat cgtcgatcag
     1801 cgcggccatg gctcgtctca acaccgcaag tgaaggcatc gtcctgctgc tcggtgacca
     1861 gccaggcgtc tcggcggaca cggtacgtcg gctcatcgac gaaggcggca cgtcgcaagt
     1921 tgcggtctgc cgctacgcgg atggtcgcga acacccgttc tggttcgctc ggtcggtgtt
     1981 cgacgagctg cgcacgttgc acggtgacaa ggcggtctgg aaggttctcg agtcgggtcg
     2041 gcactccgtt gctgaagtcg cgatccagag catggcgccg atcgacgtcg acacgtgggc
     2101 cgactacgag gcattgctgg ccgcaagcgg ggtgtcccca tagccacgcc actccatgat
     2161 gccgccgacg tgcagcgccg cctcgacgag atggactatc tcgtagac
//
LOCUS       DATCBJ010000281         2782 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_369949, whole genome shotgun sequence.
ACCESSION   DATCBJ010000281 DATCBJ010000000
VERSION     DATCBJ010000281.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2782)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2782)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2782
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_369949"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..308
                     /locus_tag="VII50_05700"
     CDS             <1..308
                     /locus_tag="VII50_05700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HEY5050374.1"
                     /translation="NAARAALLSQALTRRPDLLFVATQDALHQSYRASAMSRSAALMT
                     RLREAGIAAVISGAGPSVLALTTTANADAVAEFDTRWFTATRLAIDTEGARVLPLAG"
     gene            626..2650
                     /gene="rho"
                     /locus_tag="VII50_05705"
     CDS             626..2650
                     /gene="rho"
                     /locus_tag="VII50_05705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HEY5050375.1"
                     /translation="MTETTDAPTLAASPEGDAETGGARTKRRTAATGLTSMVLPELKA
                     LAATMGISGTGAMRKGDLIAAIQGGQQSSATPQGGSAANGSAATPPRAARSPRRAAAQ
                     AVTDVTAAPDAAADVPPSFERSSSNGSDNHVSEQGSERAYVPTQGTGQRQGSDRQGGQ
                     NRNRNRDRSDRDRSERTETAQGGQSDSGQHTQNNQAGQSSPAGQGAQHTQGDRQTNQN
                     SQNNQNRQSNQGDRDRQNNQNNQNRQNNQGGQGQPRDDDEFDSSGRRRRGRFRERNRS
                     ARSGPGRERFGSEAEPVVSEDDILVPVAGILDILDNYAFVRTSGYLPGANDIYVSLAQ
                     VRRYGMRKGDAVTGVVRQPKDNERREKFNAMVRLDTVNGTDPEKAKARPEFGKLTPLY
                     PQDRLRLEGDPGQLTTRVIDMVAPIGKGQRGLIVSPPKAGKTMVLQAIANAITRNNPE
                     CHLIVVLVDERPEEVTDMQRSVKGEVIASTFDRPAEDHTMVAELAIERAKRLVELGHD
                     VVVLLDSITRLGRAYNTAAPASGRILSGGVDSAALYPPKRFFGAARNIENGGSLTILA
                     TALVETGSRGDEVIFEEFKGTGNMELKLDRKFADKRIFPAVDVDASGTRKEELLMAPE
                     ELQIVWKLRRVLHALDSQQALELLLSKMKETKSNAEFLLQIQKTTAMPID"
BASE COUNT          532 a          944 c          879 g          427 t
ORIGIN      
        1 ctaacgcagc acgagccgcg ctgctctcgc aggcactcac ccgccgtcct gaccttttgt
       61 tcgtggcgac gcaggatgcc ctgcaccagt cctaccgagc gtcggcgatg tcgcgcagtg
      121 cggcattgat gacgaggttg cgtgaggccg ggatcgctgc ggtgatctcc ggcgcagggc
      181 cgtcagtact tgccctgacg acgaccgcca acgcagatgc cgtcgccgag ttcgacactc
      241 ggtggttcac ggcgacccgg ctggcgatcg ataccgaagg ggcgcgtgtt ctgcctctcg
      301 caggttgaga ggtgctaggg tgggccttgc accagtggtg catgactctt cgcacccggt
      361 ctggatgctt gctccccacc gacccgttga tttcagttga tttcagggcc gcccctcttt
      421 cttggagcgg tacaggtctc gaggtggagt cgagtcacag ctccgcagag ctggtgctcc
      481 ccgtgtaaac cgctatggac agttcgccat agctcccaaa gaaccgccgt ggcgcaatgc
      541 ccggcgcgtt gaaagtggct cccagcgcga tcaggtgatt ttctacacca gcctcgacgc
      601 tatgggcctt ttcggaagga ttcgagtgac cgaaacaaca gacgccccga ccctcgccgc
      661 gtcgcctgaa ggcgatgccg agaccggagg cgcacgcacc aaacgacgca cggccgcaac
      721 gggcctgacc tcgatggtgc tgcccgaatt gaaggcgctt gccgccacca tgggcatcag
      781 tggcaccggc gcgatgcgca agggtgacct catcgccgcc attcagggcg gccagcaatc
      841 atctgccact ccgcagggcg gctcagccgc caatggcagc gcggctacgc cgccgcgagc
      901 ggcccgcagc cctcgccgcg ccgccgctca ggcagtcaca gacgtcacgg ccgcaccgga
      961 cgcagctgcc gacgtcccgc ctagctttga acgttccagc tccaacggct cggacaatca
     1021 cgtctccgag cagggttccg agcgtgccta tgtgccgact caggggactg ggcagcgtca
     1081 gggctcggat cgacagggcg gccagaaccg caaccgcaac cgcgaccgat ctgaccgtga
     1141 ccgctccgag cggaccgaga ccgcccaagg cggtcagtcc gattcgggcc agcacaccca
     1201 gaacaaccag gcgggtcaaa gcagcccggc aggtcaagga gcccagcaca cccagggcga
     1261 ccggcagacc aatcagaaca gccagaacaa tcagaaccgg cagagcaacc agggcgaccg
     1321 cgaccggcag aacaatcaaa acaaccagaa ccgccagaac aaccaaggcg gccagggtca
     1381 gccgcgcgac gacgatgagt tcgacagcag cggacggcgg cggcgcggtc gtttccgcga
     1441 gcgcaaccgc agtgcccgca gcggtccggg tcgcgagcgc ttcggcagcg aagccgagcc
     1501 ggttgtcagc gaggacgaca tcctggtgcc cgttgcgggc atcctcgaca tcctcgacaa
     1561 ctacgcgttc gtccggacgt cggggtacct gcccggtgcc aacgacatct acgtctcgct
     1621 cgcccaggta cgccgctacg gcatgcgcaa gggtgacgcg gttaccggcg tcgttcggca
     1681 gccgaaggac aacgaacggc gcgagaagtt caacgcgatg gtgcgtctcg acacggtgaa
     1741 cggcaccgac ccggagaagg ccaaggcgcg tccggagttc ggcaagctca cgccgctgta
     1801 cccgcaggac cggctccgcc tcgagggcga ccccggccaa ctcaccactc gtgtcatcga
     1861 catggtcgcg ccgatcggca agggacagcg tggcctgatc gtctcgccgc ccaaggccgg
     1921 caagaccatg gtgttgcagg cgatcgcgaa cgcgatcacc cgcaataacc ccgagtgcca
     1981 cctgattgtc gtgcttgtcg acgagcggcc ggaggaagtc accgacatgc agcgctcagt
     2041 gaagggcgag gtcattgcct cgaccttcga ccggccggcc gaagaccaca ccatggtcgc
     2101 cgaactggcc atcgagcggg ccaagcgtct cgtcgagctc ggtcacgacg tcgtcgtcct
     2161 gcttgactcg atcacccgcc tcggccgcgc gtacaacacc gctgctccgg cgtccggccg
     2221 gatcctgtcc ggtggtgtcg actcagcggc gctctacccg ccgaagcggt tcttcggcgc
     2281 ggcccgcaac atcgagaacg gcggatcgct gacgatcctc gccaccgcgc tcgtcgagac
     2341 cggctcgcgt ggtgacgaag tgatcttcga ggagttcaag ggcactggca acatggagct
     2401 gaagctcgac cggaagttcg ccgacaagcg catcttcccc gcggtcgacg tcgatgcctc
     2461 cggtacccgc aaggaagaac tcctgatggc gccggaagaa ttgcagatcg tctggaagct
     2521 gcgtcgggtg ctgcacgcgc tcgactccca gcaggcgctc gagttgctgc tcagcaagat
     2581 gaaagagacc aagagcaacg ccgagttcct gctgcagatc cagaagacga cagcgatgcc
     2641 gatcgactag ctcgtctgtt cgcgggtcac ttccaggcga gcgctgccat cagcgccttt
     2701 ccgatggcga tcgcaccggt gtcttgatta aggctgtcgg ctccttcgat cgcgacgagc
     2761 cacttgccgg tctggacgtc ga
//
LOCUS       DATCBJ010000282         2449 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_330037, whole genome shotgun sequence.
ACCESSION   DATCBJ010000282 DATCBJ010000000
VERSION     DATCBJ010000282.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2449)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2449)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2449
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_330037"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..402)
                     /locus_tag="VII50_05710"
     CDS             complement(<1..402)
                     /locus_tag="VII50_05710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009079376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT domain-containing protein"
                     /protein_id="HEY5050376.1"
                     /translation="MSAGGADDIDVSGAVGVPDGLDRIWTPHRMAYIRGESKPVGDPD
                     DPALCPFCLIPAAPDADSLVVARGVLVYAVLNLYPYNAGHLLVVPYRHVPDVSELEVD
                     EAADFMAFTQRAVSTIRAVSSAEGFNIGMNLG"
     gene            complement(399..2369)
                     /gene="thrS"
                     /locus_tag="VII50_05715"
     CDS             complement(399..2369)
                     /gene="thrS"
                     /locus_tag="VII50_05715"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HEY5050377.1"
                     /translation="MSEITVTVAGAAASVPADATAADVLGSDHGPDRRTVAARINGEL
                     RDLAASVSDGDVIEAVTIDCPDGRSILRHSTAHVMAQAVQDLFPGTLLGIGPPVENGF
                     YYDFAPHHPFAPDDLARIEKRMFEIVKEDQIFRRRVVTDDDARVELADEPFKLELIGL
                     KGGTADDESVEVGGTELTMYDNVRRDGSLAWKDLCRGPHVPSTRAIPALKLMRSAAAY
                     WRGSEKNPQLQRIYGTAWESRDALKAYLAMLVEAEKRDHRKLGLELDLFSFPDELGSG
                     LAVFHPKGGVIRKEMEDYSRAKHLAAGYEFVNTPHITKSKLYEISGHLDWYADGMYPP
                     MELDGGTNYYLKPMNCPMHILIYRARGRSYRELPLRMFEFGTVYRYEKSGVIHGLTRV
                     RGMTQDDAHIFTTVDNMRDELRKLLTFVLELLRDYGLDDFYLELSTKNPDKYVGDDEV
                     WETATATLAEVAAESGLELVPDPGGAAFYGPKISVQARDAIGRTWQMSTIQLDFNLPE
                     RFELEYQGADGERHRPVMIHRALFGSIERFFAVLTEHYAGAFPPWLSPVQAVGIPITD
                     THVAYLEGAVGDALRAAGIRFEVDASDDRMQKKIRNAQKQKVPFMLIAGDDDLAAGAV
                     SFRYRDGTQKNGVAIADAVAEIRDAVERRVQV"
BASE COUNT          429 a          791 c          788 g          441 t
ORIGIN      
        1 gccgagattc atcccgatgt tgaatccctc tgcactgctc accgcgcgga tcgtgcttac
       61 ggcccgttgc gtgaaggcca tgaagtcggc cgcttcgtcg acctcaagct cgctgacatc
      121 tggcacgtgc cgatacggga cgacgagcag atggccagcg ttgtacgggt aaaggttcag
      181 tacggcgtag accaggacgc cgcgtgcaac cacgagtgag tccgcgtcag gcgcagcggg
      241 gatcaggcag aacggacaaa gagccgggtc gtcgggatcg ccgaccggct ttgactcacc
      301 gcgtatgtag gccatccggt gcggcgtcca gatgcgatcc aggccgtcag gcacgccgac
      361 cgcgccggac acatcgatgt cgtcggctcc gcctgcgctc acacctgcac ccggcgctcg
      421 acggcgtcgc ggatctcggc aacagcatcg gcgatcgcga ccccattctt ctgggtgccg
      481 tcgcgatacc ggaacgacac cgcaccggcg gcgaggtcgt cgtcgcccgc aatcagcatg
      541 aacggcacct tctgcttctg cgcgttgcgg atcttctttt gcatccggtc gtcggacgca
      601 tcaacctcga acctgatgcc ggcagcacgc agcgcatcgc caacggcacc ttcgaggtag
      661 gccacgtgcg tgtccgtgat cggaataccg acggcttgga cgggcgacaa ccatggcggg
      721 aaagcgccgg catagtgctc ggtgagtacc gcgaagaagc gctcgatcga accgaacaat
      781 gcgcgatgga tcatcaccgg ccggtgccgc tcgccatccg cgccttggta ttcaagctcg
      841 aaccgttccg gcagattgaa gtcgagctgg atggtcgaca tctgccaggt gcggccgatg
      901 gcatcgcggg cctggaccga gatcttcggc ccgtagaacg ctgcgccacc gggatctgga
      961 acgagctcaa gacccgactc ggccgccacc tcggccagcg tcgccgtcgc ggtctcccac
     1021 acctcgtcgt caccgacgta cttgtcaggg ttcttcgtcg agagctcgag gtagaagtcg
     1081 tcgagcccgt agtcacgcaa cagctcgagt acgaacgtga gcagcttgcg cagctcgtcg
     1141 cgcatgttgt caacagtggt gaagatgtgc gcatcgtcct gggtcatgcc acgcacgcgg
     1201 gtgaggccgt ggatcacgcc cgacttctcg tagcggtaca cggtgccgaa ctcgaacatc
     1261 cgaagcggca gctcgcgata cgaccgcccg cgcgcgcggt agatcaagat gtgcatcggg
     1321 cagttcatcg gtttgaggta gtagttcgtg ccgccgtcga gctccatcgg tggatacatg
     1381 ccgtcggcgt accagtcgag gtggcccgag atctcgtaga gcttgctctt ggtgatgtgc
     1441 ggggtgttga cgaactcgta cccagcagcg aggtgcttgg cgcgggagta gtcctccatc
     1501 tccttgcgga tcacgccgcc cttgggatgg aagaccgcga gcccggagcc gagctcatcg
     1561 gggaagctga agaggtcgag ttcaagcccg agtttgcggt ggtcgcgctt ctcggcctcg
     1621 accagcatcg cgaggtaggc cttgagcgcg tcgcgcgatt cccaggcggt gccatagatc
     1681 cgttgcagct gcgggttctt ctcgctcccc cgccaatacg ccgccgcgct gcgcatcagc
     1741 ttcaacgccg ggatcgctcg ggtactgggg acgtgcggac cccgacacag gtctttccac
     1801 gccagtgagc cgtcgcgccg aacgttgtcg tacatggtta gctcggtgcc gccgacctcg
     1861 acgctctcgt cgtcggccgt gccgcccttc agcccgatca gctcgagctt gaacggctca
     1921 tcggcgagct cgacgcgcgc atcgtcgtcg gtgaccaccc ggcgcctgaa gatttggtct
     1981 tccttcacga tctcgaacat ccgcttttcg atccgcgcga gatcgtccgg cgcgaacggg
     2041 tgatgtggcg cgaagtcgta gtagaagccg ttctcgacag gtggcccaat gcccagcagc
     2101 gtgccgggga acagatcctg cactgcttgc gccatcacgt gggccgtgga gtggcgcagg
     2161 atcgagcggc catcgggaca gtcgatcgtt acggcctcga tgacgtcacc gtcgctcacg
     2221 gaagccgcca gatcacgcag ctccccgttg atcctggccg ccacagttct gcgatcaggc
     2281 ccgtgatcac taccgaggac atcggccgcc gtcgcgtccg cgggcacgct ggcagcggcg
     2341 ccggcaacgg tgacggtgat ctcggacacg aaagctccct gacggcatcg gtagatgagt
     2401 ctccgatgct agcgagcctt acgagagcgg cgccggaagt tgatccgcg
//
LOCUS       DATCBJ010000283         3318 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_107035, whole genome shotgun sequence.
ACCESSION   DATCBJ010000283 DATCBJ010000000
VERSION     DATCBJ010000283.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 3318)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3318)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3318
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_107035"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(41..1495)
                     /locus_tag="VII50_05720"
     CDS             complement(41..1495)
                     /locus_tag="VII50_05720"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: protein motif:HMM:NF010086.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HEY5050378.1"
                     /translation="MNPVASEAVASGPVSRVTRKLLADGETAVGLYRKLAQDRPGTFL
                     LESAENGRSWSRYSFIGVRAAATLTEQDGRAHWIGDPPPGVPTVGTPLDVLAGTVAAL
                     RGPRDASLPPLVGGMVGFVTYDAVRRLERLPDDTIDDLHLPELVMFLVTDLAVLDHHD
                     GSVLLIANVLPGDSAENAEKRLDEMTVALQSPAPSTIAIMSDAITPPPLRRTSREQYM
                     SWVERAREYIRAGDIFQVVPSQRFETTTTVSALDIYRVLRARNPSPYLFLLRFDGFDV
                     VGSSPEAHVTVMDGRATMHPIAGSRPRGATPEDDNALAAEMLADPKERAEHVMLVDLA
                     RNDLGRISTPGTVEVVDFMTVERYSHIMHIVSTVVGQVDPAHDALDVLAATFPAGTLS
                     GAPKVRAMEIIEELEPTRRGLYGGVVGYVDFAGDLDTAIAIRTAVVRDGTVYVQAGAG
                     LVADSDPATEDAECRHKANTVLSAVAIAQALRTP"
     gene            complement(1512..1952)
                     /gene="hisI"
                     /locus_tag="VII50_05725"
     CDS             complement(1512..1952)
                     /gene="hisI"
                     /locus_tag="VII50_05725"
                     /EC_number="3.5.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-AMP cyclohydrolase"
                     /protein_id="HEY5050379.1"
                     /translation="MDPVHAAPGSPHVGALNIDLETPASAAARLDPQIIARLKRDEHG
                     LFPAVAQQYDTGEVLMVGWMDDEALRRTLTTGRCTYWSRSRQEYWVKGETSGHQQWVK
                     SVALDCDGDTVLVQVDQIGAACHTGDRTCFDAGHLPAVVGVAAT"
     gene            2087..2287
                     /locus_tag="VII50_05730"
     CDS             2087..2287
                     /locus_tag="VII50_05730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5050380.1"
                     /translation="MIETERTGVNPAIPVSPVRTTRKEIRRRVRRQVMLRRLSRELAS
                     ADDLETVLRRPHASWPQPRRET"
     gene            complement(2452..3225)
                     /gene="hisF"
                     /locus_tag="VII50_05735"
     CDS             complement(2452..3225)
                     /gene="hisF"
                     /locus_tag="VII50_05735"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HEY5050381.1"
                     /translation="MTTTDALLAVRVIPCLDVDAGRVVKGVNFVDLRDAGDPVEMARV
                     YDAEGADELVFLDITASSGDRETTYDVVRRTAEQVFIPLTVGGGVREVDDFNRLLRAG
                     ADKVGVNTAAVLRPALLAEAADRFGAQCVVLSIDARRAATSSGFEVTTHGGRQGTGID
                     AVAWAAEATELGAGEILLNSMDADGTLAGFDLELIAAVRAVASVPIIASGGAGAASDF
                     APAVAAGADAVLAASVFHFGALRIGDVKAALRAAGQPVR"
BASE COUNT          545 a         1133 c         1128 g          512 t
ORIGIN      
        1 tgcgtcgccg cggccaccgc cggtacggaa tcggtcgagg tcacggcgtg cgcagcgcct
       61 gcgcgatcgc aaccgccgac agcaccgtgt tggccttgtg ccggcactcg gcgtcctcgg
      121 tggccgggtc agaatcggct accaggccgg caccggcctg gacgtagacc gtgccgtctc
      181 gaaccaccgc cgtgcggatc gcgatcgccg tgtcgaggtc gcccgcgaag tcgacgtaac
      241 cgaccacccc gccgtacaga ccgcgccggg tgggttcgag ttcttcgata atttccatcg
      301 cccggacctt cggtgcgcca gaaagcgtcc cggccgggaa ggtcgcggca agcacgtcca
      361 gcgcatcgtg cgcaggatcg acctgcccga cgacggtcga cacgatgtgc atgatgtggc
      421 tgtagcgctc gaccgtcatg aagtcgacga cctcgaccgt gccgggcgtg ctgatccgac
      481 cgagatcgtt acgggccagg tcgaccagca tgacgtgctc ggcccgctct ttaggatccg
      541 cgagcatttc cgcggcgagt gcgttgtcgt cttcgggcgt tgcaccgcgc ggccggctcc
      601 ccgcgatcgg atgcatcgtc gcccggccgt ccatcaccgt cacgtgtgct tcgggactcg
      661 agccgacgac atcgaagccg tcgaatcgca acagaaacag gtacggactc ggattgcgcg
      721 cgcgcagcac ccggtagata tcgagcgcac tgaccgtcgt cgtggtctcg aaccgttggc
      781 tcggcacgac ctggaagatg tcaccggcgc ggatgtattc acgggcccgc tcgacccatg
      841 acatgtattg ctcgcgcgag gtgcggcgca ggggtggcgg cgtgatcgcg tcgctcatga
      901 tggcgatcgt cgacggcgct gggctctgta gcgccacggt catctcgtcg aggcgcttct
      961 ccgcgttctc ggctgaatcg ccgggtagca cattggcgat cagcagcacg ctgccgtcgt
     1021 gatgatcgag caccgcgagg tcggtcacga ggaacatcac cagctcgggc aggtgcagat
     1081 cgtcgatcgt gtcgtcgggc aaccgttcga gtcggcgcac ggcgtcgtag gtcacgaagc
     1141 ccaccatgcc cccgaccagc gggggcagcg aggcatcgcg tgggccgcgc agcgccgcga
     1201 cggtgccggc gagaacgtcg agcggagtgc caacagtcgg gacgccgggg ggcgggtcgc
     1261 cgatccaatg ggcccgcccg tcctgctcgg tgagtgtcgc cgccgcgcgc acgccgatga
     1321 acgagtaacg cgaccacgag cgcccattct cggcggactc cagcaaaaag gtgcccggcc
     1381 ggtcctgggc aagcttgcgg tagaggccga ccgcggtctc accgtcggcg aggagcttgc
     1441 gggtgacccg gctgacgggt ccggacgcca ctgcctcgct cgcgaccggg ttcatgaggc
     1501 tgccgcggca ctcatgtggc agccacgccg acgacggcgg gcaggtggcc ggcatcgaaa
     1561 caggtgcggt cgccggtgtg gcaggccgcg ccgatctggt cgacctgcac cagcaccgta
     1621 tcgccgtcgc agtcgagggc aaccgacttc acccattgct ggtggcctga ggtctcgccc
     1681 ttgacccagt actcctggcg gcttcgactc cagtaggtgc agcggccagt ggtcaaggtg
     1741 cgacgcaagg cctcgtcgtc catccagccc accatcagca cctcgccggt gtcgtattgc
     1801 tgggcgacgg cggggaacag cccgtgctcg tcacgcttga gccgggcgat gatctgcggg
     1861 tcgagccggg cagccgctga ggccggagtt tccaggtcga tgttcagtgc accgacgtgt
     1921 ggggaacctg gagcagcgtg gacgggatcc atgcagtcat tgtgccgggg cggggtgctc
     1981 gccgtcgcac gagatacggc cgttcggcgg actacgtaag acccgatggt ggatgcgcaa
     2041 cggtggtggc cgagttgaca atagggatga aacttctcgg acaggcatga tcgagactga
     2101 aaggaccggt gtgaaccccg cgatccccgt gtccccggta cggacgactc gcaaggagat
     2161 ccgccgtcgt gtccgccgcc aggtgatgtt gcgccggctg tcgcgtgaac tcgcgagcgc
     2221 cgacgacctc gagaccgtct tgcgccgccc gcacgcctcc tggccgcaac cgcgccgcga
     2281 aacttagtcg ctaccgcgac cgaagccagc gttcggcctg cgacggaatg accggagcct
     2341 ggatcgcgcg gttgtacgaa cgcgtcgacc acgcacggaa ttcgcgaacc agcagccgga
     2401 ccagcgccat gccaccaggc taccggccgg ctggtctccg gtggtcacgg ttcagcggac
     2461 cggctggccg gctgcccgta gcgcggcttt gacgtcaccg atgcgcaagg caccgaaatg
     2521 gaacacgctc gccgccagca cggcgtcggc accggcggcg acggccggcg cgaagtcgga
     2581 cgcggctccc gcacctccgc tggcgatgat cgggaccgac gccacggccc gaaccgccgc
     2641 gatcaactcg aggtcgaatc cggccaacgt gccgtcggca tccatcgagt tcagcaagat
     2701 ctcacccgcg cccagctcag ttgcctcggc agcccatgca accgcgtcga taccggtgcc
     2761 ctgacgacca ccgtgggtgg tgacctcgaa gccagacgat gtcgctgcac gccgggcgtc
     2821 gatcgacagc accacgcact gcgccccgaa gcggtcggcg gcttccgcga gcagcgcggg
     2881 ccgtagcacc gcggcggtat tcacaccgac cttgtcggca cccgcgcgca gcagccgatt
     2941 gaagtcatcg acctcgcgga ctcccccacc gacggtcagc gggatgaaga cctgctcggc
     3001 ggtgcggcgc accacgtcgt aggtggtttc gcggtcgcca ctgctggccg tgatgtcgag
     3061 gaacaccagc tcgtcggcgc cctcggcgtc gtacacccgg gccatctcga ccgggtcacc
     3121 cgcgtcgcgc aagtcgacga agttcacgcc tttcaccact cggcccgcgt cgacgtccag
     3181 gcagggaatg acgcggacgg cgagcaacgc gtcggttgtc gtcacgactg cgcgcctcgg
     3241 taggcagtgg cttcgatctc caccagaacc gtcggatcga tcagcgccga gacctcgacc
     3301 atcgtgctga ccgggcgg
//
LOCUS       DATCBJ010000284         2126 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_532200, whole genome shotgun sequence.
ACCESSION   DATCBJ010000284 DATCBJ010000000
VERSION     DATCBJ010000284.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2126)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2126)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2126
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_532200"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..286
                     /locus_tag="VII50_05740"
     CDS             <1..286
                     /locus_tag="VII50_05740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HEY5050382.1"
                     /translation="TVSVLGIAAWMRYVTARHDDAGRPLPIDDPLEPRIADVVGQSAD
                     PTTIVERLLGLTEVFGDLREDIALRGWLTEALAELTRAGALAALRHATVV"
     gene            406..1779
                     /locus_tag="VII50_05745"
     CDS             406..1779
                     /locus_tag="VII50_05745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011718889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric reductase-like transmembrane
                     domain-containing protein"
                     /protein_id="HEY5050383.1"
                     /translation="MTSVLPERGIRRSTSPSVPRSGVRPDVLLGVLSLGGALVVLLWW
                     HDTPATLHTLGDWLTNAGRITGLLAGYVIIVLLLLMARVPALERGLGADRLARWHAMG
                     GRYAVFLAVAHTLLIIWGYAVLDHQSVIPQTGALLTHYPDVMMATVALGLLVLVAGVS
                     MRVARAHMRYETWYYLHFYTYLAVALSFSHEFSTGLDFTASESARALWSLLYISIAIL
                     MFWYRFVVPIRAAMRHRMVVAGVFPEAPGIFSVHVAGHHLDDLRAEPGQFFRWRFLSS
                     GSWWQSHPYSLSAPPTDTLLRITVKVLGDHSHALRTLHKGTRVFAEGPYGALTEQRRT
                     RRQVLLLAGGIGVTPLRALFETLSARPGDLTLMYRASTELDVVFRRELDAIAQWRGAR
                     VVYLVGPPGGDADPFIGRRLASIVPDLTAHDIYLCGPPRMMDAAHSALHRVGVRRRQI
                     HTESFEF"
     gene            1826..>2126
                     /locus_tag="VII50_05750"
     CDS             1826..>2126
                     /locus_tag="VII50_05750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5050384.1"
                     /translation="MRRFSTVFAATVAGLVVLLSLKTTQAKAPRPAALSGVTPGDSST
                     PLNSSAPASSAPVSSAPVSSAPVSSAPVSSAPAGAPQPSTAAPSSSAATASKVSPS"
BASE COUNT          321 a          685 c          675 g          445 t
ORIGIN      
        1 cacggtcagc gtgctgggaa tcgcggcctg gatgcggtac gtcactgccc gccacgacga
       61 cgcgggccgc ccgttgccca tcgatgatcc gctcgaaccg cgaattgccg acgttgttgg
      121 gcagtcggcg gacccgacca cgatcgtcga acgcctgctg ggcctcacgg aggtgttcgg
      181 ggatctccgt gaggacatcg cgctgcgcgg ctggctgacg gaggcgctcg ccgagctcac
      241 ccgcgccggc gccctcgctg cattgcgtca cgccacggtg gtgtgaaccg tttccataga
      301 aagatcaccg cagctttcag cttttgcaca ggttcggagg tgacgatggt ttaggcagca
      361 acaccgtgac cctcggttcc gatcagaggt gaagccggag gtgcaatgac gagcgtgctt
      421 cctgagcggg gtattcgccg cagcacgtca ccgtccgtgc cacgttccgg cgtccggccg
      481 gacgtgctgc ttggcgtgct gagtctgggc ggcgccctgg tggtgttgtt gtggtggcat
      541 gacactccgg ccacgttgca taccctcggc gactggctga caaacgctgg tcgcatcacc
      601 ggcctgttag ccggctacgt gatcattgtg ttgttactgc tcatggcccg ggtgccggcc
      661 ctcgagcgag ggctcggcgc ggaccgcctc gcccggtggc acgcgatggg cggccgctac
      721 gccgtcttcc tggcggtcgc gcatacgttg ctcatcatct ggggttatgc cgttttggac
      781 catcagtcgg tgattccgca aacaggtgcg ttgttgacgc actatcccga cgtcatgatg
      841 gcgactgtcg cgctcggctt gctggtcctg gtcgccggcg tatcgatgcg cgtggcacgc
      901 gcgcacatgc gctatgaaac gtggtattac ctgcatttct acacgtacct tgccgtagcc
      961 ctttcgttca gccacgagtt ctcgaccggg ctcgacttca ctgcgagcga gagcgcgcgt
     1021 gccctatgga gcctccttta catcagcatc gccatcttga tgttctggta caggttcgtc
     1081 gtaccgatcc gtgccgcgat gcggcatcgc atggtggttg cgggcgtttt cccagaagct
     1141 ccagggatct tttcggtgca cgtcgctggc caccatctcg atgacttgcg tgccgaacct
     1201 ggtcagttct tccgctggcg gttcttgagc agtggtagct ggtggcagtc gcatccgtac
     1261 tcgctgtccg cgccgccgac cgacacgttg ttgcgcatca cggtgaaggt gctcggcgat
     1321 cattcgcacg cattgcgcac cttgcacaag ggcacgcggg tgtttgccga aggaccgtac
     1381 ggcgccctca ccgagcaacg ccggacgcgg cgacaggtgt tgctactcgc cggaggaatt
     1441 ggagtaacac cgttgcgagc tctgttcgag acgctctctg cacgacccgg cgaccttacc
     1501 ttgatgtacc gcgcttcgac cgaactcgac gttgtctttc gccgagagct cgatgcgatc
     1561 gcccagtggc gcggcgcgcg agtcgtctac ttggtggggc cacctggtgg tgatgcagat
     1621 ccctttatcg gacgtcgact ggcgtccatc gtgccggatc tgaccgctca tgacatctac
     1681 ctgtgtggac ccccacgcat gatggatgcg gcgcacagtg ccctgcaccg tgtcggcgtg
     1741 cgccgccgac agatccacac cgagtcgttc gaattctagg gtcgttcgag tttcagcccg
     1801 ttccagtcca ggaggaaggt gagtcatgcg ccgattcagt accgtctttg ccgcaaccgt
     1861 agccggactc gtcgtcctgc tgagtctcaa gaccacgcag gccaaggcgc cgcgtccggc
     1921 ggcgctctct ggggtcacac cgggtgattc gtctacgcca cttaactcat cagcaccggc
     1981 cagctcggca ccggtcagct cggcaccggt cagctcggca ccggtcagct cggcaccggt
     2041 cagctcggca ccggcgggtg ctccgcagcc gtcgaccgcc gcgccgagca gttcagcagc
     2101 tactgcgtcg aaggtttcgc cgtccg
//
LOCUS       DATCBJ010000285         2965 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_190948, whole genome shotgun sequence.
ACCESSION   DATCBJ010000285 DATCBJ010000000
VERSION     DATCBJ010000285.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2965)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2965)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2965
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_190948"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2965)
                     /locus_tag="VII50_05755"
     CDS             complement(<1..>2965)
                     /locus_tag="VII50_05755"
                     /inference="COORDINATES: protein motif:HMM:NF024777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LamG domain-containing protein"
                     /protein_id="HEY5050385.1"
                     /translation="VWSQAIVGNTVYAGGSFANARPAGAAAGTNLTPRSNFLSYDITT
                     GALNTSFVPNLNAQVLTVADSPDGSRVYVGGDFTVANGSSRLRIAAYSTATGQLVTTF
                     APSLNGRVHAMIATNSTVYVGGSFSTANGVARSKLAAFSAINGALLSWAPSADAGINA
                     MVMAPDQSKVIVGGQFTTLNGASWYGMGALDPVTGASLPWAADATVQDGGVNAAIDTL
                     STDGTNIYGAGFVFGAGGNLEGTFAANPANGNIVWIEDCHGDSYSTYESGSVLYLAGH
                     PHFCDDVPGGFPQTTPWTFHRTLAFGTAVTGTLLHNTQGGYADFGGSPAPTQLDWYPT
                     ITSGTYTGQNQGPWDVTGNGQYVVEGGEFPSVNGVGQQGLVRFAVSAIAPNKQGPVVT
                     GANFVPSLVSLTAGTVRVAFQANWDEDNTALTYKVVRDSNTAAPVYQTTVNSTFFNRP
                     ALGFTDTGLVAGQTYRYRLYVNDPFGNTVAGDTVSITAGSNTLSPYAQAVLYDAPVTY
                     WRLGESSGLTAYDWAGYTDGIVGSGVTRGAPGAIIGDSNTASTFNGTATSSVISPTSQ
                     PGPNSFSIEAWIKTTTNTGGKIIGFGDSNTGDSGSYDRHLYMDNAGQIIFGVYPGQTV
                     TITSGASYNDGQWHHVVATMGSAGMALYVDAKRVGQNAAVTTAQAYNGYWKIGGDNIN
                     GWPNQPTTEDFTGSIQDVAIYGAPLTAAQVTSHYIASGRTAPVGTRPTDLYGSTVFDA
                     APDAYWRLDETSGTVAKDITPNGANGNYSGGVTFGVPGAISGTTDTAITLNGINGLVS
                     SALSTTAPSVYSEEVWFKTTTTSGGKIIGFGSSPTGASGNYDRHVYMINDGQLIFGTW
                     TGQENLVTTPASYNDGHWHLLVATQGSDGMKLYVDGAVVGTNPQTAAQAYDGYWRVGG
                     DTTWGGASSDYFAGSVDDVAVYSSELSGAQVADHFAKGKGAANVLPTAAFTSTATNLN
                     ATFNAGGSTDTDGTIT"
BASE COUNT          491 a          900 c          996 g          578 t
ORIGIN      
        1 gtgatggtgc catcagtgtc cgtcgacccc ccggcgttga acgtggcgtt caggttggtt
       61 gctgtagagg tgaaagccgc ggttggcagc acattcgccg cgccttttcc cttcgcaaag
      121 tggtcggcga cctgggcgcc cgagagctcg gacgagtaga ccgcgacgtc gtcaacgctg
      181 ccggcgaagt agtcgctgct ggcgccgccc cacgtcgtgt caccgccgac tcgccagtag
      241 ccgtcgtagg cctgggccgc ggtctgcggg ttggtcccga caaccgctcc gtcgacgtac
      301 agcttcattc cgtcggaacc ttgggtggcc accagaaggt gccagtgacc gtcgttgtag
      361 ctcgccggtg tcgtgaccag gttctcctgg ccggtccagg tgccgaagat cagttggccg
      421 tcgttgatca tgtacacgtg acggtcgtag ttgccactcg caccagtcgg cgagctgccg
      481 aaaccgatga tctttccgcc gctggtcgtg gtggtcttga accagacctc ttcgctgtat
      541 accgagggcg ccgtggtgct caaggcggac gagacaaggc cgttgatgcc gttgagcgtg
      601 atcgccgtgt cggtcgttcc ggagattgca ccagggacgc cgaacgtgac gccgccgctg
      661 tagttgccat tcgcaccgtt cggcgtgatg tccttcgcca ccgtgcctga ggtctcgtcg
      721 aggcgccagt aggcgtcagg agctgcatcg aacacggtcg aaccgtagag gtcggtcgga
      781 cgcgtaccga ccggcgcggt gcgaccgctt gcgatgtagt ggctcgtgac ctgcgctgcg
      841 gtcaacggtg cgccatagat ggcgacgtct tggatgcttc ccgtgaagtc ctcggttgtc
      901 ggctggttcg gccatccgtt gatgttgtcg ccaccgatct tccagtagcc gttgtacgcc
      961 tgagctgtgg tgacagccgc gttctgcccg acgcgcttcg catcgacgta cagcgccatg
     1021 ccggcgctgc ccatggtggc cacgacgtgg tgccattgac cgtcgttgta cgacgcaccg
     1081 ctggtgatcg tcacggtctg acccgggtag acgccgaaga tgatctgccc agcgttgtcc
     1141 atgtagagat ggcggtcgta gctgccgctg tcgccggtgt tgctgtcacc gaagccgata
     1201 atcttgccac cggtgttcgt ggtggtcttg atccatgcct cgatggagaa cgaattcggc
     1261 ccgggctggc tggtcggcga gatcaccgag ctggtcgcgg tgccgttaaa ggtggacgcc
     1321 gtgttgctgt cgccgatgat ggcgccgggc gcgccgcggg tcacgccact gccgacgatg
     1381 ccgtcggtgt agcccgccca gtcatacgcg gtgaggccgg aggactcgcc gaggcgccaa
     1441 tacgtaaccg gggcatcgta gagtacggcc tgcgcatacg ggctcagcgt gtttgacccg
     1501 gcagtgatgc tgaccgtgtc cccggcaacg gtgttgccga acgggtcgtt gacgtaaagg
     1561 cggtacctgt acgtctggcc ggcgacgagg ccggtgtcag tgaacccgag tgccgggcgg
     1621 ttaaagaacg tcgagttcac cgtcgtctga tagaccggcg cggcagtgtt gctgtcgcgc
     1681 acgaccttgt acgtcagagc cgtgttgtct tcgtcccagt tcgcctggaa agccacccgc
     1741 acagtccccg cggtgagcga cacgagactt ggcacgaagt tcgcgcctgt gacaactgga
     1801 ccttgcttgt tcggcgcgat cgcgctgacg gcgaaccgca cgaggccctg ctggccaact
     1861 ccgttgacgg acggaaactc gccaccttca acgacgtact gcccgttgcc cgtgacatcc
     1921 cacggccctt ggttctggcc ggtgtaggta ccggaagtaa tggtcgggta ccagtcgagc
     1981 tgggtgggcg ccggtgagcc gccaaagtcg gcgtacccgc cctgagtgtt gtgcagcaaa
     2041 gtcccggtca cggcggtgcc gaaggcaagt gtgcggtgga aggtccaggg tgtggtttgt
     2101 ggaaatcccc cgggaacgtc gtcgcaaaag tgcggatgcc ctgcgaggta gagaacgctt
     2161 ccggattcgt aggtcgagta tgagtcgcca tggcagtcct cgatccagac tatgttgccg
     2221 tttgccgggt tggccgcgaa cgtgccttcg aggttgccgc ctgcgccgaa aacgaagcct
     2281 gcaccgtaga tgttggtgcc atcggtcgac aacgtgtcga tcgcggcatt gaccccaccg
     2341 tcctgaacgg tcgcgtcagc ggcccacgga aggcttgcgc ccgtgacagg gtcgagcgcg
     2401 cccatgccgt accagcttgc gccgttgagc gtcgtgaact gaccgccgac gatgaccttg
     2461 gactgatcgg gtgccatgac catggcgttg ataccggcat cagcggacgg cgcccacgac
     2521 aacagcgcac cgttgatagc actgaacgcg gcgagtttcg agcgcgcaac accgttcgcc
     2581 gtgctgaatg agcccccgac atacacggtc gagtttgttg caatcatcgc gtggacgcgg
     2641 ccgttgaggc tcggtgcgaa ggtcgtgacc agctggccgg tcgccgtgct gtaggccgcg
     2701 atgcgcaaac ggctggagcc gttagcgacc gtgaagtcgc caccgacata gacacgcgag
     2761 ccatcaggtg agtccgcaac ggtgagcacc tgggcattca agttcggcac gaacgacgtg
     2821 ttgagcgcgc ccgtcgtgat gtcgtaggac agaaagttgc tgcgcggggt cagattcgta
     2881 ccggcggcgg cacccgcggg cctagcgttc gcgaaactgc cacccgcgta aaccgtgttc
     2941 ccgacgatgg cttgggacca cacca
//
LOCUS       DATCBJ010000286         9844 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_215020, whole genome shotgun sequence.
ACCESSION   DATCBJ010000286 DATCBJ010000000
VERSION     DATCBJ010000286.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 9844)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9844)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9844
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_215020"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..301)
                     /locus_tag="VII50_05760"
     CDS             complement(<1..301)
                     /locus_tag="VII50_05760"
                     /inference="COORDINATES: protein motif:HMM:NF013328.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase family
                     protein"
                     /protein_id="HEY5050386.1"
                     /translation="MPEGNTVWLTARALRTTLAGHPLTHVDLRLADLATTDLVGQRVL
                     DVVPRGKHLLIRLEPALTLHTHLRMDGRWRIHPAGYRLHDPDDHVRVILGNTGGTA"
     gene            complement(337..4791)
                     /locus_tag="VII50_05765"
     CDS             complement(337..4791)
                     /locus_tag="VII50_05765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEY5050387.1"
                     /translation="MTRKKMSDADQPASDLPDGFSAVTRAWFTSAFATPTPAQVQAWE
                     AIGRGENTLVVAPTGSGKTLAAFLWSLDRITAAPIPADPARRCRVLYISPLKALAVDV
                     ERNLRAPLVGMRHAAIRLGLPEPTIEVALRSGDTPAVERRKLTTKPPDILITTPESLF
                     LILTSAARESLLGIGTVIVDEVHAVAGTKRGAHLALSLERLDAMLEKPAQRIGLSATV
                     RPVDEIARFLSGPRQATVVQPPAVKTFDISVVVPVVDMDSPDEATDEEPRKSIWPHVE
                     ERIADLIDTHRSTIVFANSRGLAERLTHRLNDIGFERGRETEIARAHHGSVSREQRSI
                     TEEMLKSGQLPAVVATSTLELGIDMGAVDLVVQVESPPSVASGLQRIGRAGHQVGAVS
                     RGVIFPKYRGDLVQTAVVAERMKVGAIESIRIPRNPLDVLAQHLVSMTAMQTWDVDDA
                     LALVRRAAPFAALPQSAFDAVLDMLAGRYPSDEFAELRPRLVWDRIAGTIAGRPGAQR
                     LAVTSGGTIPDRGLFGVFLAGEGTSRRVGELDEEMVYESRVGEVFTLGSSSWRIVEIT
                     HDQVLVVPAPGVPGKLPFWHGDAQGRPLELGRALGAFVRELSALPRPAAHTRLLDAGL
                     DDWAASNLLAYLAEQTEATGQLPDDRTIVVERFRDELGDWRVVVHSPFGAQVHAPWAL
                     AIGARLRERYGVDVQTMHADDGIVLRIPETDEAPPAQLALLASDEVDDAVTREVGGSA
                     LFAARFRECAARALLLPRRRPDRRTPLWQQRQRSAQLLSVASEYGSFPIVLETMRECL
                     QDVFDVPGLVELMRDIEARRVRLVDVETPAASPFARSLLFGYIAAFIYEGDAPLAERR
                     AQALALDSVLLGELLGQAELREMLDADAIDEIERELQHLAAERMVRDVEGVADLLRLI
                     GPLSTAEAVERGATPRWLAELEESRRAIRIRITAEERWAAIEDAGRLRDALGSPLPVG
                     IPDVFTESVNDPLGDLVSRYARSHGPFTTADVAQRFGLGPAIVTGVLERLTAAGRLKR
                     GEFRPGGSGVEWCDASVLRSIRRRSMAKLRKEVEPVPITTFGRFLPAWQHARNPLRGV
                     DGVLRTVEQLQGAVLPASALEQLVLPSRVADYTPAMLDELTVSGDVVWAGHGSLPGND
                     GWVALYPAESAPLLLPIRDTDARSTPQLHTELLDVLAGRGAMFFRELSNRVGSADDRE
                     LASTLWELVWSGLVTNDSLAPLRVLMGTSRAARHPRAPAARRGRPVMPTRAGPPTVGG
                     RWSLLPAPAIDETRRSHALAETLLDRYGVLTRGAVLAEGVPGGFAAVYRVLTAFEEAG
                     RCRRGYFVEGLGAAQFASPGAVDRLRTGTPSDDESPRAIVLASTDPANPYGSALPWPT
                     RQGEREGHRPGRKAGALVVLVDGALAVYVERGGKTLLSFDPDGTEASPQLFMRTAVEA
                     LALAVRDGWLGRLDVERADGGSVLDSPLGAALEAAGFRATPRGLRLRS"
     gene            complement(5039..5224)
                     /locus_tag="VII50_05770"
     CDS             complement(5039..5224)
                     /locus_tag="VII50_05770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5050388.1"
                     /translation="MHLISTAGDSTPVPYAPGANQSLPGFIGFTTTKLGFTVTDGTGA
                     QLWRTTDGGLTWTVVTF"
     gene            complement(5215..5979)
                     /locus_tag="VII50_05775"
     CDS             complement(5215..5979)
                     /locus_tag="VII50_05775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5050389.1"
                     /translation="MSAIGPNAYWVLGSAPCAAGQCATLERTTDAGATFTRIGRNGAG
                     GEMPPVPTTVEDGTTVRDVRFGDAQNGWLYGGAFYTTHDGGSTWSADTALPNDVVDVA
                     AASGHVWAVALSAQNGSESYGVYHATYGSSGTGTWTKVPLGTSIGQPGLAVVKQNAFL
                     LAQAGNRQGEILSSPATARASSAIRRRAHRAGTPSPSRETVHCGFVARVTPPSASLSR
                     TIRQCTGKRLTPRPRPSRSAVSTLRTPSSRAPTGCT"
     gene            6209..6424
                     /locus_tag="VII50_05780"
     CDS             6209..6424
                     /locus_tag="VII50_05780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5050390.1"
                     /translation="MAAIAMDRDGAERACASASLLASARTCVDAIDRVRRNRYAETVR
                     RKDVALPTLKLVVAVVAHRLTTSRRTM"
     gene            complement(6399..7769)
                     /locus_tag="VII50_05785"
     CDS             complement(6399..7769)
                     /locus_tag="VII50_05785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5050391.1"
                     /translation="MTQQPPDEEFERRLRDVLSSRGLGVPVPPDAIDRIHAGARRRQQ
                     RRTAASALGAVAIIAIAAVAIGVRPHGHGSTVAGKLGTASPSPVASSAAPSSSAAVHT
                     SAPIAVSSPPSSEVPSVSTPPHAAAATPYPSAGSAVPADFTPVSVTAVNDKTYWVLGT
                     QQCGGVACTAIVSTTDAGAHFIEIAAPHAATTSVDNTTRPVFNLRFADSENGWAYGDA
                     LWTTADGGATWVDTSLPAGSVKDLEAARNPGAVAGTAWAIVQPVPFTSGYQLWQAPYA
                     TGSGTGSWQRVDLGADPPGDTAPSLVVKGATAYLLGGDAKNVEHVYIASVGATTTTYA
                     APCGTATGAFSAGIGSLWTACPGGATVSTDGGATWHLAATPSQITVGVVGGIDATTAV
                     VNQGSNLVVVSTTGKPKTASISSKNANFSFLGFTDAKVGYALSQAPNRLWRTTDGGTT
                     WSAVTL"
     gene            complement(7766..8353)
                     /locus_tag="VII50_05790"
     CDS             complement(7766..8353)
                     /locus_tag="VII50_05790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SigE family RNA polymerase sigma factor"
                     /protein_id="HEY5050392.1"
                     /translation="MQPAGGVRRRQYVQEPGVMVDEERRQVFDEFARARMPELLRFGH
                     ALTGSPHAAADLVQDALERTMLAWARVENQGDPEGYVRRIMVNRNISIWRRHRRETLM
                     LEIPEDAGRRGAGHAEPVVRDLALWDRIRALPPRQRTVIVLRYYEDMSEAQIAALMGS
                     SVGTVKSQASRALASLRRDAHQFAESDGPSEGVGR"
     gene            complement(8395..>9844)
                     /locus_tag="VII50_05795"
     CDS             complement(8395..>9844)
                     /locus_tag="VII50_05795"
                     /inference="COORDINATES: protein motif:HMM:NF013714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEY5050393.1"
                     /translation="TPAVSSAAQIADLDTLAKALGSKLLRPTDAGYLTASRLYSTRFD
                     GVRPAAVAQCATTDDVQKCLAFIRATGMPVAARCGGHSYAGYSTTSGLVINVGPMKQV
                     KATGANTATVGAGATLVDVYAGLTPQHRSVPGGSCPTVGITGSTLGGGVGVVARKYGL
                     SCDAVTAIDLVTVDGQAIRCTENSEADLYWAHRGGGGGNFGIVTAIEFQTHATQSLTH
                     FLVRWDWSHADDVVTAWQEWLPTTPNELWSSLHIDGAASALDTPHVYASGVFVGSPSG
                     LSPLLDSLQSASKAPMTTRYVQAGDYLSTMFVEGGCASLTTPECHRADIDPGGKLGRE
                     ANAARSDFIASPLTSAGVDVLLTGIEKRHTLNLPGGSVLFDAYGGAINAIAPSDTAFV
                     HRDKLACLQYVAPWATSAPPSVVAANQAWLDGVYGQMRPYVSGYAYLNYIDAKLADWQ
                     HAYYGANLARLTSVKAAADPTNLLAFAQGLPT"
BASE COUNT         1575 a         3210 c         3440 g         1619 t
ORIGIN      
        1 ccgcggtccc gcctgtatta ccgaggatca ccctgacgtg gtcgtcggga tcatgcaggc
       61 ggtatccggc cggatggatt cgccagcggc cgtccatgcg cagatgcgta tgcaaggtca
      121 gcgcgggctc gagtcggatc agcagatgct tgccgcgcgg caccacgtcg agcacgcgct
      181 gcccgacgag atcggttgtt gcgaggtcgg cgagccgcag gtcaacgtgg gtgagcgggt
      241 gccctgcaag ggtcgtgcgc agcgcacgcg ccgtgagcca caccgtgttg ccttcaggca
      301 tcggtcaccg attcgtgcgc atactgacga gcctgttcac gagcgcagcc gcaggccgcg
      361 aggggttgcg cggaaccccg cagcctccag tgcagcacct aagggtgagt cgagcaccga
      421 tccgccgtcg gcgcgttcga cgtcgagccg tccgagccag ccgtcgcgga cggccaaagc
      481 aagcgcttcg acggcggtgc gcatgaacag ctggggcgac gcctccgtgc cgtcggggtc
      541 gaacgacagc aacgtcttcc cgccgcgctc gacatacacg gcgagcgcgc cgtcgacgag
      601 cacgacgagc gcaccagcct tccgtccggg ccgatgccct tcgcgctcgc cctgccgcgt
      661 gggccagggc aacgccgatc cgtacggatt cgccgggtcg gtcgacgcga ggacgatggc
      721 gcgcgggctt tcgtcgtctg acggcgtgcc ggtacgcagc cggtcgactg cgcctggcga
      781 ggcgaactgc gcggccccga ggccttcgac gaagtacccg cgccgacagc ggccggcctc
      841 ttcgaatgcc gtgagcaccc gatagaccgc ggcgaacccg ccggggacac cctcggccag
      901 taccgcgccc cgagtcagga cgccgtatcg atcgagcagc gtctcggcca gcgcgtgcga
      961 gcgcctcgtt tcgtcgatcg ccggcgctgg cagcagactc cagcgaccgc cgaccgtagg
     1021 cggacctgcg cgggtgggca tcaccggtct accgcgccga gcggctggcg ctcgcgggtg
     1081 acgcgcggct cggctcgtgc ccatcagtac gcgcagtgga gccagcgagt cgttggtcac
     1141 cagacctgac cagacgagct cccacaaagt gctcgccagc tcgcgatcgt cggccgagcc
     1201 gacccgattc gacagctcgc gaaagaacat cgcgccgcga ccggccagca cgtcgagcaa
     1261 ctcggtgtgt agctgcggtg tcgatcgcgc gtccgtgtcg cggatcggca gcagcagcgg
     1321 cgcggactcg gcggggtaca gcgcgaccca gccgtcgttg cccggcagcg atccgtgccc
     1381 ggcccacacg acgtcgccac tgactgtgag ttcgtcgagc atcgccgggg tgtagtcagc
     1441 tacgcgcgac ggcaacacca gttgttccag cgccgacgcg ggaagaaccg caccctggag
     1501 ttgttcgacg gtgcgcagca cgccgtcgac tccgcgcagc ggattgcgtg cgtgctgcca
     1561 tgcaggcagg aagcggccga acgtggtgat aggtaccggc tcgacttctt tgcgaagctt
     1621 cgccatcgag cgacgccgga tggaacgcag cacgctcgcg tcacaccatt cgacccccga
     1681 accgccaggt ctgaactccc cacgcttcag ccggccggcc gcggtcagtc gttcgaggac
     1741 gcccgtgacg atggccggcc caagcccgaa gcgttgcgca acgtcggccg tagtgaaggg
     1801 cccgtggctg cgcgcgtagc gcgacaccag atcgccgagt gggtcattga ccgattcggt
     1861 gaacacgtca ggaatgccga caggcaaagg gcttcccagt gcatcgcgca agcggccggc
     1921 gtcctcgatc gccgcccatc gctcctcggc agtgattctg atgcggatcg cgcggcggct
     1981 ttcttccagt tcggccagcc agcgcggtgt cgcgcctcgc tcgaccgcct ctgcggtgga
     2041 gagcgggccg atcagccgaa gaagatccgc gacaccttcg acgtcgcgga ccatgcgttc
     2101 ggctgcgagg tgttgcagtt ctcgttcgat ctcgtcgatc gcatcggcgt cgagcatctc
     2161 tcgcagctcc gcctgaccga gaagttcacc tagcagcacg gagtcgagcg cgagtgcttg
     2221 cgcacgacgt tcggcgagcg gagcgtcgcc ttcgtagatg aacgccgcga tgtagccgaa
     2281 gagcagcgag cgtgcgaacg gggatgctgc tggcgtctcg acgtcgacca gacgcacccg
     2341 ccgcgcctcg atgtcgcgca tcaactccac gagaccggga acgtcgaaga cgtcctgaag
     2401 gcattcacgc atggtctcaa gcacgatcgg gaacgatccg tattcgctcg cgaccgacag
     2461 tagctgtgcg gaacgctggc gttgttgcca caatggcgtg cgacgatcgg gacgccgacg
     2521 gggcagcagc aacgcacgcg cggcgcactc tcggaaccgg gcggcgaata acgccgagcc
     2581 gccgacctcg cgggtgaccg catcatcgac ctcgtcggag gcaagcagcg cgagctgggc
     2641 cggtggggct tcgtcggttt cggggatgcg cagcacgatg ccgtcgtcgg cgtgcatcgt
     2701 ttggacgtcg acgccgtatc gttcacgcaa tctggcgccg atggccagtg cccatggggc
     2761 atgtacctgc gccccgaacg gtgaatggac gacgacgcgc cagtcgccga gctcgtcgcg
     2821 gaaccgctcg acgacgatgg tgcggtcgtc aggcaactgg ccggtcgctt cggtctgttc
     2881 agccaggtag gcgagcaaat tcgacgcggc ccaatcatcg agccctgcgt cgagcagccg
     2941 ggtgtgtgcg gccgggcgtg gcaaggccga gagctcgcga acgaacgcac cgagcgcgcg
     3001 cccgagctcg agcggccgac cttgggcgtc gccgtgccag aagggcagct tgccgggcac
     3061 gccgggcgcg ggcacgacca gcacttggtc gtgggtaatt tcgacgatgc gccacgacga
     3121 cgagccgaga gtgaacacct cgccgacgcg ggactcgtag accatctcct cgtcgagctc
     3181 gccgacgcgt cgcgatgtgc cttcgccggc gaggaaaacg ccgaacaggc cgcgatccgg
     3241 gatcgtgccg ccgctggtga cagccagccg ttgcgcgccg ggccggccgg cgatggtgcc
     3301 cgcgatgcgg tcccagacca gacgcggccg cagttcggcg aactcgtcgc tcgggtagcg
     3361 gccagcgagc atgtcgagca ccgcatcaaa ggcggactgg gggagtgccg caaatggcgc
     3421 ggcgcgtcgg acgagcgcga gcgcatcgtc gacgtcccac gtttgcatcg ccgtcatgga
     3481 cacaaggtgt tgcgcgagca cgtcgagtgg gttgcgcgga atccggatcg actcgatcgc
     3541 gccgaccttc atccgttcgg cgacgaccgc ggtctgcaca aggtcgccgc ggtacttcgg
     3601 gaagatcact cctcgcgaaa cagcgcccac ttgatggccg gcccgaccga tgcgctgcag
     3661 tccgcttgcc acgctgggtg ggctctcgac ctgcacgacg aggtcgaccg cacccatatc
     3721 gatgccgagt tcgagggtgc tcgtggccac caccgcgggc agctgcccgg acttcagcat
     3781 ttcctcggtg atgctgcgtt gttcacggct cactgagccg tgatgagctc gcgcgatctc
     3841 ggtttcgcgg ccgcgctcga agccgatgtc gttcagccga tgggtaagcc gctcagcaag
     3901 acctcgcgag ttcgcgaaga cgatcgtcga tcggtgagta tcgatgaggt cggcgatgcg
     3961 ttcttcgacg tgcggccaga tcgacttgcg tggctcttcg tcagtcgcct cgtcggggga
     4021 gtccatgtca acgactggga cgacgacgga gatgtcgaac gtcttcaccg caggcggctg
     4081 gacgacggtc gcctgccgcg gcccgctgag aaatcgggcg atctcgtcga ctggtcgcac
     4141 tgtcgccgac aagccaatgc gttgcgcggg cttctcgagc atcgcgtcaa ggcgttcgag
     4201 cgagagcgcg agatgcgcgc cgcgtttggt gccggcgacc gcatgcactt cgtcgacgat
     4261 cacggtgccg atgcccagca gggactcgcg tgccgcagag gtgaggatca agaacagcga
     4321 ctcgggcgtg gtgatcaaga tgtcgggcgg cttggtcgtg agcttccggc gttcgaccgc
     4381 tggcgtgtcg ccggagcgca acgcgacctc gatcgttggc tcgggcagcc cgaggcggat
     4441 ggccgcgtgc cgcatgccga ccagtggtgc gcgcaggttg cgttcgacgt cgaccgcgag
     4501 cgccttcaac ggcgagatat acagcactcg gcagcgccgc gccggatcgg ccggaatcgg
     4561 tgccgcagtg atgcggtcga gcgaccacag aaaggccgcg agcgtcttgc ccgaaccggt
     4621 cggtgccacc acgagcgtgt tctcgccgcg cccgatggcc tcccaggcct gtacctgagc
     4681 cggcgtcggc gtcgcgaagg cggaggtgaa ccaggctctg gtgaccgcgg agaagccatc
     4741 aggcaagtcg gacgccggct ggtctgcgtc cgacatcttc ttccgggtca tgccagtgat
     4801 tctggacctt gccaccgaca gccgccgcac catgtgctcg tggatgggtg gggcgtggga
     4861 cgcatcgggc taggggcgct gctggctggc gtcgcgctga ccggctgcag cggtggtcct
     4921 tcgcaggcgt ctgcgcccgc cgcccgccgt cgtcgatccg accgagcagc gcagtccgaa
     4981 gttggaagcc tatgggcgct ctcttgagcg tctagggact accaagttag cggttcgact
     5041 agaaagtgac gacggtccag gtcaggccgc cgtccgttgt acgccaaagc tgagcgccgg
     5101 tgccgtcggt cacggtgaag ccaagcttgg tcgtggtgaa gccgatgaag ccgggcaggc
     5161 tctggttggc gcctggtgcg tacggcaccg gggtggagtc gcctgcggtg gagatcaggt
     5221 gcaacccgtc ggtgctctcg atgagggcgt gcgtagcgtc gacaccgccg accgagacgg
     5281 ccgcggacgc ggcgttagcc gcttgccagt gcactgcctg atcgttcgag acaaagacgc
     5341 tgatggtggt gtcacccgag caacgaatcc acagtgcacc gtctcccgcg acggagatgg
     5401 cgttcccgct cgatgcgcac ggcgccggat ggctgacgag gctcttgccg tcgccggcga
     5461 tgacaagatc tcgccttgtc tatttcccgc ctgcgccagc aagaatgcgt tttgcttgac
     5521 gaccgcaagt ccgggctggc cgatcgacgt cccgagcgga accttcgtcc aggtaccggt
     5581 tcccgagctg ccgtaagtcg cgtgatagac gccgtacgac tcacttccgt tctgcgcgct
     5641 gagtgcgacg gcccacacat gtccgctcgc cgcggcgacg tcgacgacgt cgttgggaag
     5701 cgcggtgtcc gcgctccatg tcgatccgcc gtcgtgggtc gtgtagaacg cgccaccgta
     5761 gagccagccg ttttgggcgt ctccgaagcg gacgtcgcgg accgtcgtgc cgtcctcgac
     5821 ggtcgtcggg acgggcggca tctcgccacc ggcgccgttg cggccgatcc gggtgaacgt
     5881 cgcgccggcg tcagtggtgc gttcgagagt cgcgcactgt ccggccgcgc atggagctga
     5941 ccccagcacc caatacgcgt tcgggccgat agcgctcacc gacagcggtt gaaacgagga
     6001 tggtgtcgcc gatgtctgcg tcggggacac gaccggtggc gggacgacga cagcggacgc
     6061 cgacccagca ggtgacgggg taatagacgg tgacggacta acaaacggcg acgccggcat
     6121 gcttgacgag acggcgacag acgggcttgg cgagctcgtg ttcgcagtca ctgccgatcc
     6181 gtgaccgtgt ggccgcaccc cgatggcaat ggccgcaatc gcgatggatc gcgacggcgc
     6241 cgagcgcgct tgcgccagtg cgtcgttgct ggcgtcggcg cgcacctgcg tggatgcgat
     6301 cgatcgcgtc cggcgaaaca ggtacgccga gacggtgaga agaaaggacg tcgcgttgcc
     6361 gacgctgaag ctcgtcgtcg ccgtcgtcgc tcatcggctc acaacgtcac ggcggaccat
     6421 gtagtgccac cgtctgtcgt tcgccataac cgatttggcg cctggctgag ggcgtatccg
     6481 accttggcgt cagtgaatcc caggaaagag aagttcgcgt tcttgctgct gatcgatgcg
     6541 gtcttcggct tgccagtagt cgacaccacc acgaggttcg atccctggtt caccaccgca
     6601 gtcgtcgcgt cgatcccgcc gacgactccg acggtgattt gcgatggggt cgcggccaag
     6661 tgccacgtcg cgccaccatc ggtcgagacg gttgcgccgc ctgggcacgc ggtccacagt
     6721 gatccgatgc cggccgagaa tgcccccgta gcggtgccgc acggtgcggc gtacgtcgtg
     6781 gttgtcgcgc ccaccgatgc gatgtacaca tgttctacgt tcttggcatc gccgccgagc
     6841 aggtaggccg tggcgccttt cacaacgagg ctcggggccg tgtcgcctgg cggatcggca
     6901 cccaggtcga cccgctgcca ggagccggtc ccggagccgg tcgcgtacgg tgcctgccac
     6961 aactgatatc ccgaggtgaa gggaaccggt tgaacgatgg cccaggccgt cccggcgacc
     7021 gccccgggat tgcgcgccgc ctcgaggtct ttcacactcc cggccggcaa cgacgtatcc
     7081 acccaggtgg caccgccgtc cgcggtcgtc cacagcgcgt cgccgtacgc ccagccgttc
     7141 tcgctgtcgg caaatcgaag gttgaagacc gggcgagttg tgttgtcgac gctagtggtc
     7201 gccgcgtgtg gtgcggcgat ctcgatgaag tgcgcgccgg catcggtggt gctcacaatc
     7261 gctgtgcaag ccacgcctcc gcactgctgc gtacccagaa cccaatatgt cttgtcgttg
     7321 accgcggtga cagataccgg cgtgaagtct gcgggcactg ctgaacctgc gctggggtat
     7381 ggggttgcgg ctgccgcatg cggcggcgtc gagacgctcg ggacttccga cgacggcggc
     7441 gacgaaacgg ctatgggcgc gcttgtgtga actgccgcag atgacgatgg tgcggcagat
     7501 gacgctaccg gcgacgggct cgcagtaccc agcttgccgg ccactgttga gccgtgaccg
     7561 tgcggtcgca ccccgatggc gacggcagcg atcgcgatga ttgcgacggc gccgagggcg
     7621 cttgccgcag tgcgtcgttg ctggcgtcgg cgcgcaccgg cgtggatgcg gtcgatcgca
     7681 tccggcggaa cgggaacgcc gagaccgcga gaagaaagga cgtcgcgcag gcggcgttcg
     7741 aattcctcgt ccggcggttg ttgggtcatc ggccgacccc ttccgacggt ccgtccgatt
     7801 cggcgaactg gtgcgcgtcg cggcgcagtg acgccagcgc gcgcgaggct tggctcttca
     7861 cggtgcctac cgacgacccc atcaacgcgg cgatctgcgc ctcggacatg tcttcgtagt
     7921 aacgcaacac gatgaccgtt cgttgccgcg gcgggagcgc ccggattcga tcccataacg
     7981 cgagatcgcg gaccactggt tcagcgtggc cggcacctcg gcgtccggca tcttctggaa
     8041 tctctagcat cagcgtctcg cgacggtgcc gtcgccagat gctgatgttg cgattcacca
     8101 tgatgcgccg cacatacccc tccgggtcgc cttggttctc tacccgcgcc cacgccaaca
     8161 tcgttcgctc caacgcgtcc tggacgagat cggctgcggc atgagggctg ccggtcaagg
     8221 cgtgaccgaa acgcagcagc tccggcatgc gggcgcgtgc gaactcgtcg aagacctgtc
     8281 gccgctcctc gtccaccatc accccgggct cttgtacgta ttgacgacgc cggacacctc
     8341 cggcgggttg catcggtcgt ccgacaggtt ctcgcccgag cgtagagcta ccggtcaggt
     8401 cggcaggcct tgcgcgaacg ccagcaggtt ggtcgggtcg gccgccgctt tgacgctcgt
     8461 cagccgtgcg aggttcgccc cgtagtaggc gtgctgccag tcggccagtt tggcgtcgat
     8521 gtagttgagg tacgcgtacc cgctcacgta gggccgcatc tgtccgtaga caccgtcgag
     8581 ccacgcctgg ttggccgcga cgacggacgg cggcgcgctg gtcgcccatg gggccacata
     8641 ttgcaggcag gcgagcttgt cccggtgcac gaaggcggtg tcgctggggg cgatcgcgtt
     8701 gatcgcgccg ccataggcgt cgaagagcac gctgccgccg ggaaggttga gagtgtggcg
     8761 cttctcgatg ccggtgagca ggacgtcgac gccagccgag gtcagcgggc tggcgatgaa
     8821 gtcggaccgc gccgcgttgg cctcccggcc gagcttgccg cccggatcga tgtcggctcg
     8881 gtgacactca ggtgtggtga ggctcgcgca gccgccctcg acgaacatcg ttgagaggta
     8941 gtcgcccgcc tgcacgtagc gggtggtcat cggggctttc gaggccgact gcagtgagtc
     9001 cagcagcggg gacaggccgg atggcgagcc gacgaagaca ccgctcgcgt agacgtgcgg
     9061 tgtgtccaat gccgaggcgg cgccgtcgat gtgcaggctc gaccacagct cgttcggggt
     9121 ggttgggagc cattcctgcc aggccgtcac gacgtcatca gcatgcgacc agtcccaccg
     9181 caccaggaag tgggtgaggc tctgcgtggc atgggtctgg aactcgatcg cggtgacgat
     9241 gccgaagttg ccgccgccgc cgcctcgatg cgcccagtag aggtcggcct cgctgttttc
     9301 cgtgcagcgg atggcctggc cgtcgacggt gacgaggtcg atcgcggtga ctgcgtcgca
     9361 gctcaggccg tacttgcgcg cgacgactcc gacgccgccg ccgagggttg atccggtgat
     9421 gccgacggtg ggacacgagc cgccgggcac ggaccggtgc tgcggggtaa ggccggcata
     9481 gacgtcgacc aaggtggcac cggcaccgac ggttgcggtg ttcgcgcctg tcgctttgac
     9541 ctgcttcatc ggcccgacat tgatgacaag gccggacgtc gtggagtagc ccgcgtaact
     9601 atggccgccg cagcgagccg cgaccggcat gcccgtcgcg cggatgaacg cgaggcattt
     9661 ctgcacgtcg tctgtggtcg cgcactgcgc gacagcggcc ggtcgtacgc cgtcgaagcg
     9721 ggtcgaatac agccgggacg ccgtgaggta accggcatcg gtcgggcgca gcaatttgga
     9781 gccgagggcc ttggcgaggg tgtcgaggtc ggcgatttgc gcggcagacg atacggcggg
     9841 cgtc
//
LOCUS       DATCBJ010000287         3276 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_76550, whole genome shotgun sequence.
ACCESSION   DATCBJ010000287 DATCBJ010000000
VERSION     DATCBJ010000287.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 3276)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3276)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3276
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_76550"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1361
                     /locus_tag="VII50_05800"
     CDS             <1..1361
                     /locus_tag="VII50_05800"
                     /inference="COORDINATES: protein motif:HMM:NF015049.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MBOAT family protein"
                     /protein_id="HEY5050394.1"
                     /translation="PRRARNGVLAFASLVFYTWGGHAFVFLLLGCVAVNFAAGLALDS
                     AKPFLQRHRRTALVSAIVVDVGALAVWKYADFASRQVNALVHAFGGHGSHVLHFVLPI
                     GISFFTFHHLSYVVDVYRRTRKPLRNPLTFMTYILMFPQLIAGPIVRFHEIADQLETP
                     AQDRFGDFAAGFPRFAVGLSKKVIVADSVAPVVNSVFSLHTSQLTTVNAWLGALAYTV
                     QLYFDFSGYSDMAIGLGRMFGFQLPENFNRPYSASSITDFWRRWHMSLSRWFRDYLYI
                     PLGGNRSGKWKTYRNLYIVFALTGFWHGANWTFFVWGLYHGTLLVIERVTGWGDTATR
                     WRPLQRALTQFLVVLGWVVFRANSLPQAGHILGAMFMPTGHGLTATVATALTHRTLLI
                     FWLALLVFVIPPGFVMGRFVEFGTSRPAIATKFAYVVVLAPIAAALVAVGTFSPFLYY
                     QF"
     gene            1374..2612
                     /locus_tag="VII50_05805"
     CDS             1374..2612
                     /locus_tag="VII50_05805"
                     /inference="COORDINATES: protein motif:HMM:NF028132.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5050395.1"
                     /translation="MARIPRLKVRGKVVRIAIAGLALTFFFGPAVAAAAGVRAPAIEN
                     HKLGALPNSRDGWASFGIFDTWATDHLPLRNVAISANTKVERNVFGETPNYGANGTAT
                     TTTATGVAGISVPQPPAAPASAATAAFPTVITGIDGWLYYGTDLRAACNPTLTAPVIA
                     QRFQQLSEIMQRAGKQFVMMIAPDKSDIYPQYLPTADQGVTCASKAEAALWAALAANP
                     PAGYQDLRGAIDAAKAADPGTVLYRKLDTHWNGVGALIYARTLINSLDPTLWPTATVT
                     PLGDSSSHTDLEALLGQRTTEQAPLFSVTRPGVTTTINTASQLADTTTDAPLVTPKTL
                     FISDSFTDSSRFAINPFFAQAGFINSSAASTAFDELVQSIKSAQIVVLEIVERTVIGG
                     QAPLLDSSVQAKLAASLGEG"
     gene            2684..>3276
                     /locus_tag="VII50_05810"
     CDS             2684..>3276
                     /locus_tag="VII50_05810"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HEY5050396.1"
                     /translation="MDHHRVLLPGPDGPVPVDEVVPERPARGAVIVLQEAFGVNDHIV
                     DVCRRFAAVGYRTVAPHLFHRDGVNALPCNVELALPHIANLTADGIRADLTAAREHLA
                     GQGLSLSLTGIVGFCMGGSVVCALAAEDAYGAAVTFYGSGMHKGRFGFRPLVELAPQL
                     RSPWLGLYGDIDPGIPVDQVEDLRSAAATAHVPTAVVR"
BASE COUNT          542 a         1125 c          994 g          615 t
ORIGIN      
        1 tcccgcgccg cgcgcgcaac ggggtgctgg ccttcgccag cctggtgttc tacacgtggg
       61 gcggccacgc gttcgtgttc ctgctcctcg gttgcgtcgc cgtcaacttc gcggcgggtc
      121 tcgcgctcga ttcagcaaag cccttcctgc aaagacatcg gcggacggcg ctggtgagcg
      181 ccatcgtcgt ggacgtcggt gccctcgccg tgtggaagta cgccgacttc gcctcccgtc
      241 aggtgaacgc gctcgtccat gccttcggcg gtcacggcag ccatgtgttg cacttcgtgt
      301 tgccgatcgg gatttcgttc ttcacgttcc accatctaag ctacgtcgtc gacgtctacc
      361 gccgtactcg caagccgttg cgcaatccgc tgacattcat gacctacatc ttgatgttcc
      421 cgcagctcat cgcgggcccg atcgtgcgat tccacgagat cgccgatcaa ctcgaaacac
      481 cagcgcagga tcgcttcggc gatttcgccg caggcttccc ccggttcgcg gtcggcttat
      541 cgaagaaagt catcgtcgcc gatagtgtcg caccggtcgt caacagcgtc ttctcgttac
      601 acacgagcca gctgacgacg gtcaacgcat ggctgggtgc gctggcgtac accgtgcagc
      661 tgtatttcga cttctcgggg tattccgaca tggccatcgg gctcggccgg atgttcggat
      721 tccaactccc cgagaatttc aaccggccgt actccgcatc gtccatcacg gacttctggc
      781 ggcgctggca catgtcgctg tcccgatggt ttcgcgacta cctctacatc ccgctcggcg
      841 gcaaccgaag cggcaagtgg aagacctatc gcaacctcta catcgtcttc gcccttaccg
      901 gtttctggca tggcgcgaac tggacgtttt tcgtgtgggg gctctaccac ggcacgctgc
      961 tggtcatcga gcgcgtcacc ggctggggcg acacggcgac gcggtggcgg ccgctccaac
     1021 gggcgctcac ccagtttctc gtcgtgctcg gttgggtggt gtttcgggcc aacagcctgc
     1081 cacaggccgg tcacatcctt ggcgcgatgt tcatgccaac cggccacgga ctcacggcca
     1141 ccgtcgcgac cgcgctcacc catcggacac tgctgatctt ctggctggcg ctgcttgtct
     1201 tcgtgattcc gcccggcttc gtcatgggcc gcttcgtcga gttcggcacc agccggccag
     1261 ccatcgcgac caagttcgcc tacgtggtcg tgcttgcacc cattgcagcc gcgctcgtcg
     1321 cggtcggcac cttcagcccc ttcctttact accagttcta gagaggagtg acgatggccc
     1381 ggatcccgcg gttgaaggtg cgtggcaagg tcgtgcgcat cgcgatcgcc ggcctggcat
     1441 tgacgttctt cttcgggccg gccgtagccg ccgccgccgg cgttcgtgct ccggcgatcg
     1501 agaatcacaa gctcggcgcg ctaccaaaca gccgcgacgg atgggcatcg ttcgggatct
     1561 tcgatacatg ggcgaccgat cacctgccgt tgcgcaatgt agcgatcagc gcgaacacga
     1621 aggtcgaacg caacgtgttc ggcgagacac cgaactatgg cgcgaacggc acggcgacaa
     1681 cgacgacggc gactggagtt gccggcatct ctgtgccaca accgcctgca gcgccggcga
     1741 gcgcagcaac ggccgccttc cctaccgtga tcaccggcat cgacggctgg ctgtactacg
     1801 gaaccgacct tcgggccgcc tgcaatccaa cactcacggc ccccgtcatc gcgcaacgct
     1861 tccagcaact gtcggagatc atgcagcgtg cgggcaagca gttcgtcatg atgatcgccc
     1921 ccgacaagag tgacatctat ccgcagtacc taccgaccgc agatcagggc gtgacctgcg
     1981 cgtcgaaggc agaagccgca ctgtgggcag ccttggccgc aaaccctccg gctggctatc
     2041 aggatctgcg cggagccatc gatgcggcga aggcggcgga tcccggcacc gtgctctacc
     2101 gcaagctcga cacgcactgg aacggcgtcg gcgcgctgat ctacgcacgc accctcatca
     2161 acagcctcga tccgacgtta tggccgacgg ctaccgtgac gccgctgggc gacagcagct
     2221 cgcacaccga tctcgaggca ctgctcggcc agcggaccac cgagcaggcg cccctgttct
     2281 ccgtcacccg gccgggtgtc accacgacca tcaatacggc gagccagctc gccgacacga
     2341 cgaccgatgc gccgctggtg accccgaaga cgctgttcat cagcgactcg ttcacggatt
     2401 cgtcgcgttt cgcgatcaac ccgttcttcg cccaggccgg attcatcaac tcctcggcgg
     2461 caagcaccgc cttcgacgaa ctcgtgcagt cgatcaagag cgcgcagatc gtcgtgctgg
     2521 agatcgtcga gcgtaccgtt atcggcggcc aggcacccct tctcgacagc agtgtccagg
     2581 ccaagctggc cgcgagcctc ggggagggct agcgaacacg ggcgcgcgat ggctcaactg
     2641 cgctgtggca ctctcaactg cgctgtggca ctagggtgcg gccatggatc accaccgcgt
     2701 gctgctgccc ggacccgatg gacccgttcc tgtcgacgag gtagttcccg aaaggcccgc
     2761 acgcggggcg gtgatcgtgc tgcaggaagc cttcggcgtg aacgaccaca tcgtggatgt
     2821 gtgtcggcgg ttcgcggcgg tcgggtaccg gaccgtggcg ccacatctct ttcatcggga
     2881 cggcgtcaat gcactgccat gcaatgtcga gctcgcgctc ccccacatcg ccaacctgac
     2941 cgccgacggt attcgcgcgg acctcactgc agcacgcgaa caccttgctg gccagggatt
     3001 atcgttgtcg ctgaccggca tcgtcggatt ctgcatgggt ggctcggtgg tgtgcgctct
     3061 cgcggcggag gatgcctacg gcgccgccgt caccttctat ggcagcggca tgcacaaggg
     3121 ccgcttcgga ttccggccgc tggtcgaact cgcgccacaa ttgcggtcgc cgtggctcgg
     3181 gctgtacggc gacatcgacc ccgggatccc ggtcgaccag gtcgaagatc tgcgcagtgc
     3241 agccgcaacc gcgcacgtgc caaccgccgt cgtccg
//
LOCUS       DATCBJ010000288         3428 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_11301, whole genome shotgun sequence.
ACCESSION   DATCBJ010000288 DATCBJ010000000
VERSION     DATCBJ010000288.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 3428)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3428)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3428
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_11301"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(14..529)
                     /locus_tag="VII50_05815"
     CDS             complement(14..529)
                     /locus_tag="VII50_05815"
                     /inference="COORDINATES: protein motif:HMM:NF012481.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin domain-containing protein"
                     /protein_id="HEY5050397.1"
                     /translation="MRAVVIYESMYGNTRTIAEALGDGLSWVFDTTVIPVDKATADVI
                     ADADLVAVGGPTHAHSMSRAATRQAAVDAAREKALTLDASAEGLSLREWFADLGAHPH
                     AIALAFDTRLAMPATVTGRAGRGIARRLRRCRFADVRDAGSFFVDKSDKLLAGETERA
                     AGWGRSFVRDL"
     gene            complement(562..978)
                     /locus_tag="VII50_05820"
     CDS             complement(562..978)
                     /locus_tag="VII50_05820"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEY5050398.1"
                     /translation="MTSTILLAVDDSAHSQRAADVSQGLAQLTKYKVVVIHIHELATG
                     RWGSMRVDDNTGDAFAEAIVKGLLGAGIDASIETRDAHYGQVAQTLSAAAEDLGADMI
                     VVGSRGRSNVASLTLGSVSHKLLHLARRPVLVVPSV"
     gene            complement(1107..2090)
                     /locus_tag="VII50_05825"
     CDS             complement(1107..2090)
                     /locus_tag="VII50_05825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5050399.1"
                     /translation="MDSSVVPTRVSTAHALVAAAATAPSILNTQPWSFEIRDHTIELY
                     EDSDRAILRRIDPIGRQLTISCGAALFNMRVTAAHLGRALDVVLLPDPTKPRLLATVN
                     VGARVPVRGPDAILQSAIRRRHTHRRAFSRRGVPGAIVRELVEAAQLEQATLFVMSRR
                     ERAWLLDLIALSDAVLVDDPGYDRDLARWTDGNPRRHDGIPPSSFGTTSVSGSPPMRD
                     FARANPRTSVPQQRYPADPCVAILSTSHDTPMAWLNAGQALQRVLLLATIRGLSASFL
                     NQPLETAEIRGELFPYRHPQMIMRLGYAQPDVATPRRALIEIIRRESNRGS"
     gene            2396..2986
                     /locus_tag="VII50_05830"
     CDS             2396..2986
                     /locus_tag="VII50_05830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5050400.1"
                     /translation="MRRKTFDALLTAGGLVLAAVLLVAGVLGLWAHNFIDNQVKSQLS
                     QQQIVFPAAGSAAISDPAIKPYLTKYAGQTMTNGAQAEAFADHYIAVHLLAIGGGKTY
                     SQLSAQAIADPTNTKLAGTVETMFKGETLRGLLLNGYAFWKMGQYALIAAVVAFIGAG
                     FLLVLAGLGLVHLRRTDPTAELLPKLGARTLAPVEP"
     gene            3222..>3428
                     /locus_tag="VII50_05835"
     CDS             3222..>3428
                     /locus_tag="VII50_05835"
                     /inference="COORDINATES: protein motif:HMM:NF013974.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5050401.1"
                     /translation="MLVADPAIATIDGNEAAARVAHALSEIIVLYPITPASPMGEFCD
                     AWSGAGRRNIWGAVPDVVEMQSEAG"
BASE COUNT          557 a         1050 c         1149 g          672 t
ORIGIN      
        1 agactgccgt gcgtcagagg tcccgtacga atgagcgccc ccatccggca gccctttctg
       61 tctctcccgc gagcagcttg tcgctcttgt ctacgaagaa gcttccggcg tcgcgaacgt
      121 ccgcgaatct gcacctacgt agacggcgcg caatcccacg gcccgcgcgg cccgtcaccg
      181 tagctggcat cgccagcctc gtgtcgaacg cgagggcgat cgcatgtgga tgcgccccga
      241 gatccgcgaa ccactcgcgc agactcagtc cttccgccga cgcatccagc gtcagcgcct
      301 tttcgcgcgc ggcatcgacc gccgcctgac gagttgcggc gcgagacatt gaatgcgcgt
      361 gggttgggcc gccgacggca acgaggtcgg catctgcgat gacgtcggcc gttgccttgt
      421 cgacgggaat caccgttgta tcgaacaccc aactcaaccc gtcaccgagg gcttcggcga
      481 tcgtacgggt gtttccgtac attgattcgt agatgacgac ggcgcgcatg acctgttcac
      541 ccctcaagtt cggtgcgccc gttagacgga cggaactacc aggacgggtc ggcgcgcgag
      601 gtgtagcaac ttgtgcgaca cgctgccaag cgtgagcgaa gcgacgttcg agcgacctcg
      661 tgatccgacg acgatcatgt ccgcgcctag atcctcggct gcggccgaaa gcgtttgcgc
      721 cacttgcccg tagtgggcgt cacgggtttc gatgctggca tcgataccag cccctagcag
      781 ccctttgacg atcgcttcgg cgaatgcatc gccggtattg tcgtcaaccc gcatgcttcc
      841 ccagcggccg gtcgcgagtt cgtggatgtg gatcacaacg accttgtact tggtgagctg
      901 agccagtcct tgcgagacgt cggcggcgcg ctgcgagtgc gccgagtcat cgactgccag
      961 caagatggtg ctcgtcattg cagcctcctc gatacggccc cgtgagccca cctttcaccg
     1021 gacgccagcc ccgctgcagg gcacaacgtc tgcaggtcct gggcccttgt tcccgttgct
     1081 gggcgctcga ccggtgcact tgggggctag ctgccgcgat tggactcgcg gcggatgatt
     1141 tcgataagcg cacgccgtgg ggttgcgacg tccggctgcg cataccccaa acgcataatc
     1201 atttgagggt gtcggtacgg aaacaactcg ccccggattt ccgcggtctc cagcggttgg
     1261 ttgaggaacg acgctgagag tcctcggatt gtggcgagca gtaaaacgcg ttgcaacgct
     1321 tggccggcat tgagccacgc catcggcgtg tcatgtgagg tcgacaggat cgctacgcat
     1381 gggtctgccg ggtaacgctg ctgcggcacc gaagtacggg gatttgcgcg cgcaaagtca
     1441 cgcatcggtg gtgatccgct caccgacgtc gtgccgaacg agctgggagg aatgccatcg
     1501 tgtcgccgcg ggttgccgtc cgtccagcgc gccaggtcac ggtcgtagcc cgggtcatct
     1561 accagtacgg cgtccgagag cgcaatcagg tcgagtagcc atgcgcgctc gcgccgggac
     1621 atcacgaaga gcgtcgcttg ctcgagttgg gcggcttcca ccagctcacg tacgatcgcg
     1681 ccgggcaccc ctcggcgact gaacgcgcgg cggtgggtgt gtcgccgccg gatcgccgac
     1741 tgcaggatcg catctgggcc gcgaacaggt acgcgcgccc cgacgttgac tgtcgccagg
     1801 agtcggggct tcgtcggatc ggggaggagt acgacgtcga gtgcgcgccc gaggtgggcg
     1861 gccgtcactc gcatgttgaa gagtgcggcg ccgcagctga tcgtgagttg cctcccgatc
     1921 gggtctatac gacgcaggat tgcacgatcg gaatcctcgt acagttcgat agtgtggtca
     1981 cgaatttcga acgaccacgg ctgcgtattg aggatcgacg gcgcagttgc ggccgctgcg
     2041 acgagagcat gcgccgtgct tactcgcgtc gggacgacgg acgagtccat ggctgactcc
     2101 ttcgggccaa tctcctaccc accagcatcg acaggaccat ggatggttgg tcgagtcgat
     2161 agtcccgggt tcttgtgaca acttgccctg cggcgcaggt cacagtcgct gcacggccgg
     2221 cttgcccagc gcacagttgc actgacgggc gggttggtcc cgggtaactg gggtctatgg
     2281 cagtgctggt gggggacctt ggtcgctacg tggtgggggc gggtcggacg aacatcgtgg
     2341 tgcagggttc gacggttgtt tgccggatgg tccggctcct atgaaggagg tcgtcatgcg
     2401 ccgcaagact tttgatgcac tattgactgc agggggcctc gttctcgcgg ccgtcctgtt
     2461 ggtggccggg gtgttgggct tgtgggccca caatttcatt gacaaccagg tcaagtcgca
     2521 gttgtcccag cagcagatcg tgttcccggc ggctggttcg gcggcgatct cggatccggc
     2581 tatcaagccg tatctgacca agtacgcggg tcagacgatg accaatggtg cgcaggcgga
     2641 ggcgttcgcc gatcactaca tcgcggtgca cttgttggcg atcggtggcg gcaagacgta
     2701 ctcgcagctg tccgcgcagg cgattgctga cccgacgaac accaagttgg cgggcacggt
     2761 ggaaaccatg tttaagggtg agacccttcg tggtctgctg ctgaacgggt acgcgttttg
     2821 gaagatgggc cagtacgcgc tgatcgccgc tgtggtggcg ttcatcggtg ccgggttctt
     2881 gctggtgctg gccgggttgg gcctggtgca cctgcggcgc accgatccga ccgcggagct
     2941 gctccccaag ctcggtgcgc ggaccctggc tcccgtcgaa ccgtaacggc gaccgcaccg
     3001 gcgatgaggg cccccgccca cgcgagggcc ctcatctgcg tctgcgttgt ctcacgtcca
     3061 aggtccgttg gttgcgagtc tcgacgttcg gtggcttggt gcctcgcacg ctgggacgtt
     3121 cgaccctcac agcaccgatg actcaatgcc tacgtttgct cttggaagcg ctgatgtggc
     3181 gccgccaagc tcacctgctt ttttccgcta tggaggctca gatgttggtc gcagacccgg
     3241 cgatcgcgac gatcgacggt aacgaagctg ccgcacgggt cgcgcacgcg ctgtcggaga
     3301 tcatcgtgct gtacccgatc acgccggcgt cgccgatggg tgagttctgt gacgcctgga
     3361 gtggcgctgg tcggcgaaac atctggggag cggttccgga cgtcgtcgag atgcagtccg
     3421 aggcgggc
//
LOCUS       DATCBJ010000289         2749 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_183512, whole genome shotgun sequence.
ACCESSION   DATCBJ010000289 DATCBJ010000000
VERSION     DATCBJ010000289.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2749)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2749)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2749
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_183512"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..332
                     /locus_tag="VII50_05840"
     CDS             <1..332
                     /locus_tag="VII50_05840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HEY5050402.1"
                     /translation="EPLDGAILFAPAGELVPVALRALDAGGTLAVAGIWLSDIPALNY
                     DADLFRERRLRSVTANTRRDGEEFLRLAQRFAIRATTVAYDMTDAPRALSDLAHGRFG
                     GAAVLHN"
     gene            362..1573
                     /locus_tag="VII50_05845"
     CDS             362..1573
                     /locus_tag="VII50_05845"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013253.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy acid oxidase"
                     /protein_id="HEY5050403.1"
                     /translation="MSDRQVPKWADFKPLLRVKPIEFNRTARQLAGALTISDLRAIAR
                     RRVPRGVFEYTDGAAESEISLRRARQLFRDVQFNPSVLRDVADADMTTLVLGRDASVP
                     FAFAPTGYTRMMHHEGERAVVRVAERRGIPYALSTMGTTTIEDVALAAPDARKWFQLY
                     VWTDRRVGEDLMTRAQAAGFEALILTVDVPVGASRLRELRNGFSLPPALTARTILETA
                     THPAWWLNLLTTSPLTFALLTSSGGTLGEVADKLFDPSMTIVDLEWLRSFWHGPLIIK
                     GIQTVDDARRVVDAGADAIVVSNHGGRQLDRAPVPLRLVPDVVDAVGDRTEVWVDSGI
                     MTGGDIVAAIALGARATLVGRAYLYGLMAGGERGVDRAAELLTREVRRTMQLLGVSSI
                     GELQPSHVQLP"
     gene            1650..2723
                     /locus_tag="VII50_05850"
     CDS             1650..2723
                     /locus_tag="VII50_05850"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing diguanylate cyclase"
                     /protein_id="HEY5050404.1"
                     /translation="MEQDTDLGSDDHHGDHQGTSGVDAARLLEVLRRIDCHVYIGETL
                     RGGGYREMFTGPGLEALLGGPVPAGVDPADAWVAAVHPDDFERQANADSGHRLGGPVS
                     VEYRLVGYDGVTRWVLDRMWPWPATPDGRLIYDGVVTDVTALHQTTDALHEALLVAQS
                     ANFELAHARAVAEQQARTDDLTGLRNRRDFAERLERHLTRAAYDQRPIGLLLIDIDFF
                     KQINDWHGHGAGDQLLTAFAGRLREATRPDDLIARWGGEEFVVLLTDLGSGDVLAQRA
                     EQIRKAVASTPFIVAGQAIGVRVSIGAAPSSVALATSDALLAAADGAMYEAKRAGRDR
                     VAVAPAHGGAFRPSSATADAGKH"
BASE COUNT          439 a          865 c          943 g          502 t
ORIGIN      
        1 ccgagcctct cgatggtgcg atccttttcg caccggcggg cgaacttgta ccggtagcat
       61 tgcgagcgct tgatgccggc ggcactcttg cagtggccgg gatctggctg tctgacatcc
      121 cggcactgaa ctacgacgcc gacttgttcc gggagcgccg gctgcgcagc gtcacggcca
      181 acaccaggcg ggacggcgag gagtttctgc gacttgccca gcggttcgcc atccgggcaa
      241 ccaccgtcgc ctatgacatg accgacgcgc cgagagctct ctccgacctc gcgcacggcc
      301 gctttggcgg cgccgccgtg ctgcacaact agccatcgtt ggctacctct agattcagag
      361 cgtgtctgat cgtcaggtac cgaaatgggc ggacttcaaa ccgctgctga gagtcaagcc
      421 gatcgagttc aatcggacgg cacgacagct cgccggcgcc ctgacaatca gcgacctccg
      481 cgcaatcgcc cgtcgccgtg ttccgcgagg cgtgttcgag tacacagacg gtgcggccga
      541 gtcagagatc agcttgcgac gggcccgtca gctctttcgc gacgtgcagt tcaatccgtc
      601 ggttctccgg gatgtcgcgg acgccgacat gacaacgttg gtgctgggac gcgatgcttc
      661 ggttccgttc gcgttcgcgc cgaccggcta cacgcggatg atgcaccacg agggtgagcg
      721 ggccgtcgtt cgggtggcag agcggcgagg cattccttac gcgctgtcga cgatgggcac
      781 gacgacgatc gaggatgtcg cgttagctgc cccggacgct cgcaagtggt ttcagctgta
      841 cgtctggaca gaccgccggg tgggcgaaga cctgatgacc cgagcgcagg cagccgggtt
      901 tgaggctctc atcttgacgg tcgatgtccc ggtgggggcg agccggctgc gtgagttgcg
      961 caacggattc tcgctcccgc cggcgcttac tgcgcgaacg attcttgaga cggcgaccca
     1021 tccggcgtgg tggctgaacc tgttgacgac aagcccgttg accttcgcgt tgttgacctc
     1081 gtcaggtggc actctcgggg aggtagcgga caagctgttc gacccgtcga tgacgattgt
     1141 tgacctcgag tggttgcggt ccttctggca tggcccgttg atcatcaaag ggatccagac
     1201 cgtcgacgat gcgcgacgcg tcgtcgacgc aggcgcggac gccatcgtgg tgtcgaacca
     1261 cggcggccgc caacttgatc gtgccccggt accactgcga ctcgtaccgg acgtcgttga
     1321 tgctgtgggt gaccggaccg aagtgtgggt cgacagcggc atcatgaccg gcggagacat
     1381 tgtcgcggcc attgcgttgg gcgcccgcgc gaccttggtc gggcgcgctt acctgtacgg
     1441 gctgatggcc gggggcgaga ggggggttga ccgcgcggca gagctcctca cccgggaggt
     1501 tcgccggacg atgcaacttc tcggcgtttc gtcgatcggc gagctccagc cgagccacgt
     1561 ccaactgcct taaggccgtg tcgaacggat gagtcccgtt gtggcgttca gttgtgcgtc
     1621 cgaggcaggt tgctgccgat tcagaatggg tggagcagga cacagacctg gggagcgacg
     1681 accatcacgg cgatcaccag gggacgtcgg gcgttgacgc tgcgcgcctg ctcgaggtcc
     1741 tgcgccgcat cgattgtcac gtctacatag gtgagacctt gcgcggtggc gggtaccgcg
     1801 agatgtttac cggcccaggc ctcgaggccc ttcttggtgg gccagttcct gccggcgtcg
     1861 atccagccga cgcgtgggtg gcggcggtgc atcccgacga cttcgagcgt caggcgaacg
     1921 cagactcggg tcatcgcttg ggcggaccgg tgtccgtcga gtaccgactg gtcggttacg
     1981 acggggtgac ccgctgggta ctcgaccgga tgtggccctg gccggccacg cctgatggtc
     2041 ggctcatcta tgacggcgtc gtcaccgacg tgacggcgct gcaccagacg acggatgcac
     2101 tgcacgaggc gctcttggtg gcgcagtccg cgaactttga acttgcgcac gcgcgcgctg
     2161 ttgccgaaca gcaggcacga accgacgact tgaccggcct gcgaaaccgg cgtgacttcg
     2221 ctgaacgact cgagcgccac ctcactcgcg cggcgtacga ccagcgtccg atcgggttgc
     2281 tccttatcga catcgacttc ttcaagcaga tcaacgactg gcatgggcac ggcgccggcg
     2341 accagctgct cacggcgttc gccggcaggc tgcgcgaggc gacccggccg gacgatctca
     2401 tcgcccgctg gggtggtgaa gagttcgtgg tgctgttgac ggatctgggc agcggcgatg
     2461 ttttagcgca gcgggccgag cagatccgca aagcagtcgc aagtacgccg ttcatcgtgg
     2521 cgggccaggc aatcggcgtc cgagtctcga ttggcgcggc accaagcagc gtcgccctcg
     2581 ctacgtcaga tgcgttgctc gcggccgccg acggcgccat gtatgaggcg aagcgtgccg
     2641 gccgcgaccg agtcgccgtc gcccccgctc acggcggcgc tttccggccc agcagcgcta
     2701 ccgccgacgc cgggaaacac tgatggcgtg accgccttgg tttctgggg
//
LOCUS       DATCBJ010000290         2870 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_37987, whole genome shotgun sequence.
ACCESSION   DATCBJ010000290 DATCBJ010000000
VERSION     DATCBJ010000290.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2870)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2870)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2870
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_37987"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            325..807
                     /locus_tag="VII50_05855"
     CDS             325..807
                     /locus_tag="VII50_05855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014136563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HEY5050405.1"
                     /translation="MSFNVGETVVYPHHGAALIEAIESRVINGEERIYLVLKVAQGDL
                     TVRVPADNAEFVGVRDVVGQEGLDRVFEVLRAPHTEEPTNWSRRYKANLEKLASGDVN
                     KVAEVVRDLWRRDRERGLSAGEKRMLAKARQILVSELALAEGTNEDKAEAILDEVLAS
                     "
     gene            848..2035
                     /locus_tag="VII50_05860"
     CDS             848..2035
                     /locus_tag="VII50_05860"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HEY5050406.1"
                     /translation="MTIKKRAPSGVVDVLRLLVVVFFAGLGYEVSKRFNSGAFVLGPL
                     NGVMVGIIIGSGLGYVLGGVLGRTTVTVVGRTEHTLRQASAEELVAGSFGGVIGVLVG
                     AGVAWWIFLLLQPFLAFPLFGFVVLVIGYLGYRLGSSRREEMLSMFASRAGMGPRPQA
                     TSNLPRIVDTSVAIDGRILDVVRAGFLDGTFVVPSPVLAELQGLADAGDDLRRSKGRR
                     GLQNLEALKREPGVNLEILDDDVPAVPEVDAKLVRICLDRGLPLLTLDTNLAKAAALA
                     GVHVMNLHGLTLALRPPVSAGDDVVVHLLRPGKEAGQAVGYLDDGTMVVAERSRDHIG
                     DEIAIHVTSVLTTANGRMVFGQPVVGPQAPNGSATDAVKPDPLRVETARPGPPSHPLG
                     RSL"
     gene            2032..2760
                     /gene="ispD"
                     /locus_tag="VII50_05865"
     CDS             2032..2760
                     /gene="ispD"
                     /locus_tag="VII50_05865"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: protein motif:HMM:NF013308.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HEY5050407.1"
                     /translation="MTVAAIVPAAGRGERLGPGQPKALRALDGVPMLVYAVQALARAR
                     LVELVVVAAPPDGVAEVEALLAAHDIGAPVLVVAGGEQRQDSVGRALEALPPEVDVVL
                     VHDAARPLAPSELVDAVASAVLAGHGAVVPGLAMTDTVKRVVPSNTGPDADEQPPELV
                     LETVPRTDLRCIQTPQGFDRAVLTKAHAAAEDTATDDAGLVERLGLPVVVISGSADAF
                     KITRPMDLLIAEAVLRERRGDGVR"
BASE COUNT          428 a          933 c          993 g          516 t
ORIGIN      
        1 ccggctggtg tcccgcccgc tttgtcttcg ttgccgctac tgccgctact gcccggctcc
       61 acggatccag tatgagcacg cagcccggct gcgcctagat ggggccaagg taaaggctcg
      121 tcaccgcctt gttcagggcc cggcgggccc cgctaagtgc cccagcggcg cacagttacc
      181 ccggttttgt caagacctga atcggcggtt gttgatcttg cattcgctgt cacgatggct
      241 ctgacctgcg cagatgcaga gaccgacgtt cccgcgggcc cgttttgcgt ggtaccatga
      301 ggcagcggaa ggggctagtg ccacatgtcg tttaacgtcg gcgagacagt tgtttacccc
      361 catcacgggg cagcactgat cgaagccatc gagagtcgtg tgatcaatgg cgaggagcgc
      421 atctacctcg tcctgaaggt agcccagggt gatctcacgg ttcgggtgcc cgctgacaac
      481 gccgagtttg tcggcgtccg cgacgtcgtg ggccaagagg gcctcgatcg ggtgttcgag
      541 gtgcttcgcg caccgcacac cgaagagccc accaactggt cgcgccgcta caaggccaat
      601 ctcgagaagc tggcgtctgg cgacgtcaac aaggtggccg aggtcgttcg cgacctttgg
      661 cgccgtgacc gcgagcgtgg gctgtctgcg ggcgagaagc ggatgctcgc gaaggcccgg
      721 caaatcctgg tcagcgaact ggcgcttgcc gaaggcacga atgaggacaa ggccgaagca
      781 atccttgacg aggtcctcgc ctcctaacat gggttagcgc cgggagcccg gccgcccctg
      841 gagggtcatg accatcaaga agcgcgcccc tagcggggtc gtcgacgtcc tgcgcctgct
      901 cgtcgtcgtg tttttcgcgg ggctcggcta tgaggtctcc aagcgcttca actcgggcgc
      961 cttcgtgttg ggtccgctca acggcgtcat ggtcggcatc atcatcggct ccggcctcgg
     1021 gtacgttctc ggtggcgtgc tcggtcgcac gaccgtcacc gtggtgggcc gcaccgagca
     1081 caccctgcgc caggcatcgg ccgaagaact cgtcgcgggc tcgttcggtg gcgtgatcgg
     1141 ggtgctcgtc ggcgcgggcg ttgcgtggtg gattttcctg ctgctacagc cgtttctcgc
     1201 gtttccgctg ttcgggttcg tcgtgcttgt catcggttat ctcgggtatc gcctcggcag
     1261 cagccgccgt gaggagatgc tttcgatgtt cgccagccgc gccggcatgg gcccgcggcc
     1321 gcaggcaacg tcgaacctgc ctcgcatcgt cgacacgtcg gtggcaatcg atggccggat
     1381 tctcgacgtc gtgcgcgcag ggtttctcga cggcacgttc gttgtgccaa gtccggtact
     1441 cgcggagctg caaggcctcg ccgatgccgg tgacgacctg cgtcgttcga agggacgacg
     1501 cgggctacag aatctcgaag cgttgaagcg ggagccaggg gtcaacctcg agatactcga
     1561 cgacgacgtg ccggccgtgc cagaggtcga cgcgaagctg gtacggatct gcctcgaccg
     1621 cggacttccc ctgctgactc tcgacaccaa cctggccaag gccgctgcgc tggccggcgt
     1681 ccacgtcatg aatctgcatg gcctcacact cgcgctgcgc ccgccggttt cggcaggaga
     1741 cgacgtggtc gtccatctgc tgcgtcctgg caaagaggcg ggccaggccg tcgggtacct
     1801 cgacgacggc acgatggtgg tggccgagcg gtcgcgcgat cacattggcg acgagatcgc
     1861 gatccacgtc acgagtgtgc tgacgacggc gaacggacgg atggtcttcg gccagccggt
     1921 ggttggtccg caggctccca acggatccgc aaccgacgcc gtgaagccgg atccgctgag
     1981 ggttgagacg gcacgaccgg gccctccgag ccatccgctc ggccgatcgt tgtgactgtc
     2041 gcggccatcg tgccggcggc gggccgcgga gagcggctcg gccccggtca gccgaaggcg
     2101 ctgcgcgcgc tcgacggcgt cccaatgctt gtctacgcgg tgcaagccct ggcgcgcgcg
     2161 cgccttgtcg agcttgtggt cgtcgccgcg cctccggacg gcgttgccga agtcgaagcg
     2221 ttgctcgccg cgcacgacat cggtgcgccc gtgttggtgg tcgctggcgg tgagcaacgc
     2281 caagactcgg tagggcgggc gctcgaggcg ctgccgcccg aggtcgatgt cgtcctggtc
     2341 catgacgcag cccggccgct cgcaccgagc gagctcgtcg acgcggtggc gagcgccgtg
     2401 ctggcaggtc acggtgcagt ggtcccgggc ctcgcgatga ccgacacggt taagcgcgtg
     2461 gtcccgtcga ataccgggcc ggacgccgac gagcagccgc cagagcttgt gctcgagacg
     2521 gtgccgcgca ccgatctgcg ctgcatccag actccgcagg gcttcgatcg cgcggtgctg
     2581 acgaaggcac acgcggcggc ggaagacacc gcgaccgacg acgccggtct cgtcgagcga
     2641 ctcggacttc ccgtcgtggt gatctccgga tcagccgacg cgttcaagat cactcgcccg
     2701 atggatctgc tgatcgccga agccgtcttg cgcgaacggc gaggtgacgg tgtccgctga
     2761 cgccggcagc gtcgcggccc tgctgccgcg ggtgggcctc ggcatcgacg tccacccttt
     2821 tgaggttggt cgcccactgt gggtagccgg gttgcactgg cccggcgaga
//
LOCUS       DATCBJ010000291         3765 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_154562, whole genome shotgun sequence.
ACCESSION   DATCBJ010000291 DATCBJ010000000
VERSION     DATCBJ010000291.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 3765)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3765)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3765
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_154562"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..638
                     /locus_tag="VII50_05870"
     CDS             <1..638
                     /locus_tag="VII50_05870"
                     /inference="COORDINATES: protein motif:HMM:NF013916.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5050408.1"
                     /translation="PLSPLNATVEEAVALGADAVGYTLYVGTPAQATDFEQYRIVRAD
                     ATRLGMPLIVWAYPRGAAITEKGGTDSFYAVDYAARAASELGADMIKVNFPRPQKRSG
                     VRAEYDDLFSDEEAIGAVVRSANRSLLLVSGGEKTGDDAMLTKARMTMEAGGTGLIFG
                     RNVWQRTHLEAMHFVAQLRAVLAEYPSPERSLVVGPTSIASTPDPRLVPVS"
     gene            766..1089
                     /locus_tag="VII50_05875"
     CDS             766..1089
                     /locus_tag="VII50_05875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5050409.1"
                     /translation="MPNLPHDVSDLYLAPVILSIDAKLGDLGILDAHALAQRVAIESN
                     RPDRSREERERALLASIEHFVDCRGWEMTWDPRGLRLTHAEHTVVLGVPANLAEYLSG
                     AAVVV"
     gene            1683..1865
                     /locus_tag="VII50_05880"
     CDS             1683..1865
                     /locus_tag="VII50_05880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5050410.1"
                     /translation="MAAWCADPYVSVDPELAPLERVLVESEMIIVGALHAVYRSLDPS
                     QPTADVWNVFGRGVGI"
     gene            1896..2648
                     /locus_tag="VII50_05885"
     CDS             1896..2648
                     /locus_tag="VII50_05885"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEY5050411.1"
                     /translation="MSVVIPAYMEGEAIVACLVRVLEAVSLPHEILVVVDSPADSTCA
                     AIEKGFAEDPSVVLLVNDYGRGPANAIRCGIDHAAAPVVVVTMADGSDDPRLIEDLTR
                     LVERGVVVAAASRYCPGGQQVGGPALKGVLSRLAGHSLHAIARVGTRDATNSFKAYSA
                     AFVREVGIDSRDGFEIGIELTAKARRLRRPVAELPTIWLDRTAGVSNFKITVWIPRYL
                     FWYRFAFGRSLDVATMRARLATKKNPHREEQE"
     gene            2653..3681
                     /locus_tag="VII50_05890"
     CDS             2653..3681
                     /locus_tag="VII50_05890"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEY5050412.1"
                     /translation="MSQQKVLISGSAGFIGGYVVEELLRRGYAVVGIDDYSKYGKVAR
                     SYDDHPEYHFVEADARDAALLTQLLLDCDHFIAGAALIGGISYFHTYAYDLLATNERI
                     IAASCDAAIKAHHDSRLQKVTYLSSSMVFESTDRWPSVEGDERTIPPPLSSYGFQKLA
                     VEYFAHAAWEQYKLPYTIVRPFNCVGIGERRAAGDVEILSGNVKLAMSHVVPDLVQKV
                     LKGQDPLRILGDGSQVRHYTYGGDLAKGIVTAMAHPAAANEDFNMSTAESTTVRELAE
                     IIWCKINGDRPLTLVSDEPFEHDVHRRVPSVEKAKRMLGYEATTTLSQMLDEAIPWIE
                     QAVADGTI"
BASE COUNT          683 a         1195 c         1201 g          686 t
ORIGIN      
        1 cgccgttgtc gccacttaac gcgacggtcg aggaagctgt ggcgctcggg gccgacgccg
       61 tcggctacac cctctacgtg ggtaccccag cccaggccac cgatttcgag caataccgca
      121 ttgtgcgggc agatgccacg cggctcggca tgccgttgat cgtctgggct tacccccgcg
      181 gggccgcgat cactgaaaag gggggtaccg actcgttcta cgcggtcgac tatgcagccc
      241 gcgccgccag cgaactcggc gcggacatga tcaaggtgaa ctttccgcgg ccgcaaaagc
      301 gcagtggtgt gcgcgcagaa tacgacgacc tgttcagcga cgaagaagcc attggtgcgg
      361 tggtgcggtc ggctaatcgc tcactgctgc ttgtttccgg cggagagaag accggtgacg
      421 acgcgatgct gacgaaggcc cgcatgacga tggaggctgg cggcacggga ctcatattcg
      481 gtcgcaacgt atggcaacgc acccacctcg aagccatgca cttcgtcgcg caactgcgag
      541 cggtactggc ggaatacccg tcaccggaaa gatcgctcgt ggtcggccca acgtccatcg
      601 cgagcacgcc cgatccccgg ttggtgccag tttcgtagca tccgcgtgtg ggatgtcaaa
      661 cgtctaggcg atgggggacc tcttaccctg ctcgccgaca tcattccgcc cgaacattgg
      721 tagggacagc gacgacaagt gcttcatgtc aacgaggagg ttccggtgcc caacctgccg
      781 cacgatgtct cggatctgta tctcgcacca gtgatcctct cgatcgatgc caagctcggc
      841 gatctcggga tattggatgc ccatgcgctc gcgcagcggg tcgctattga gagcaaccgg
      901 cccgaccgga gtcgcgagga gcgggaacgc gcactgcttg cgtcaatcga gcacttcgtc
      961 gactgccgtg gttgggagat gacgtgggat ccccgaggtc ttcggctgac ccatgccgaa
     1021 cacactgtcg tcctcggtgt gccggcgaac ctcgccgagt acctgtccgg cgcagcggtg
     1081 gtcgtctgac caaacaatga tcgggcggac accctcattt cactcggtgc cccggcagtg
     1141 cccccggcaa acgcgcgccc ggcagcagtg ccccggcagt gccccggcag tgccccggca
     1201 gtgccccggc agcagtgccc cggcaggatt cgaacctgcg cccccgcctc cggagcgcga
     1261 aagccgtcgg ccggagcgct gcggcgagct ccgcttgagc ggtgagaaac cttgtactgc
     1321 tgaaaactcg agcaatgacc atcgtttgtt gtcgatggcc attgacggcg gactacggac
     1381 gtcttgagtt gagtgcggcc tgagtgcgga ctgatcgaag atcaactagc ggcccgatct
     1441 cggtggcggg accgaactac cgccaacgtc gccgaacagc gacgttgggc ataccggcgg
     1501 tggtccgaaa ccgggctggt tgaaacgcaa caagtggccg cgaggacgca ccacctatcc
     1561 ttggtggctg tggtcctacg ccaacactcg ttcgaccttc aacgcgaacc ctcgacgcca
     1621 cgcgcgatga cctggagact gatgaaatgc gtcgatcagt ttgccgatcc ctcgtgctga
     1681 gtatggccgc ctggtgtgcc gacccttacg tcagcgtcga tccggagctg gcgccgctcg
     1741 aacgcgtcct ggtcgagtcg gagatgatca tcgtcggcgc gctgcatgcg gtctaccgca
     1801 gcctcgaccc gtcgcagccg acggccgacg tgtggaacgt attcggccgt ggggtcggga
     1861 tctgactcgt ggccgagagc aacggggatc cgcgggtgag cgtcgtcatc cccgcctaca
     1921 tggaggggga ggcgattgtg gcgtgtcttg tgcgtgtgct agaggcggtc tcgctgcccc
     1981 acgagatcct cgtcgttgtc gacagtcccg ccgacagcac ctgtgctgcg atcgagaagg
     2041 ggttcgccga ggatccgtca gtggtactcc tggtcaacga ctacggtcgc ggtccggcga
     2101 acgccatccg ttgcggcatc gaccatgccg cggcgcccgt cgtcgtcgtc acgatggcgg
     2161 acggcagcga cgatccccgc ctcatcgagg acctcaccag gctcgtcgag cgcggcgtcg
     2221 tcgtcgcggc ggcgtcgcgc tactgtccgg gtggacaaca ggtcggtgga cctgcactta
     2281 aaggtgtgct gtcgcggctg gctggccatt ctctgcacgc gatcgcgcgc gtcggcaccc
     2341 gcgatgccac gaactccttc aaggcatact ccgcggcgtt cgtccgcgag gtcggcattg
     2401 acagccgtga cggcttcgag atcgggatag agctgaccgc gaaggccagg cggctgcgcc
     2461 gtccggtcgc cgagctcccg accatctggc tcgaccggac cgccggcgtc tcgaacttca
     2521 agatcacggt gtggatcccg cggtacttgt tctggtatcg attcgccttc ggtcgttcgc
     2581 tcgacgtggc gacgatgcgt gcgcgactgg cgaccaagaa gaacccccat cgggaagagc
     2641 aggaataggc gcatgtcgca gcagaaagtc ctgatttccg gatcagccgg cttcattggc
     2701 ggctacgtcg tcgaggagtt gctccggcgc ggctacgccg tcgttggcat cgatgactac
     2761 tccaagtacg gcaaggtggc gcggtcctat gacgatcacc cggagtacca cttcgtcgag
     2821 gccgacgccc gcgatgccgc attgctgacg caattgctgc tcgactgcga ccatttcatt
     2881 gcgggcgccg cactgatagg cggcatcagc tattttcaca cttacgccta tgacctcctt
     2941 gcaacaaatg agcggatcat cgcggcctcc tgcgatgccg cgattaaggc acaccacgac
     3001 tcgaggctgc aaaaggtcac ctatctgagt tcgtcaatgg tattcgagtc gaccgaccgc
     3061 tggccttcgg ttgagggcga cgagcgcacg attccacccc cgttgtcgtc gtacggcttc
     3121 cagaaactcg cggtcgaata cttcgcgcac gccgcgtggg agcagtacaa gctgccctac
     3181 acgatcgtcc ggccgttcaa ctgcgttggg atcggggagc gacgtgcggc tggcgatgtc
     3241 gagatcctct cgggcaacgt caagctcgcg atgagtcacg tcgtgcccga tctcgtccag
     3301 aaggtgctga aaggccagga cccattacgt attctcggcg acggtagcca ggtacggcac
     3361 tacacctacg gcggtgatct ggccaagggc attgtcacag ccatggcgca ccccgccgct
     3421 gccaacgaag acttcaacat gtctacggcc gaatcgacga cggtgcgcga gctcgccgag
     3481 ataatctggt gcaagatcaa tggcgacaga ccgctcacgc tggtcagcga cgagccgttc
     3541 gaacacgacg tccacagacg cgtgccgagc gtcgagaagg ccaagcggat gctcggctat
     3601 gaagcaacga cgaccttgtc gcagatgctc gacgaggcta ttccgtggat cgagcaggcg
     3661 gtcgctgacg gcacaatctg atcatcggct gtctcgatcc gggcgagccg agcttcaatg
     3721 accatcgctt tctggctgca ttccgccgtg cggcggcggc tgcac
//
LOCUS       DATCBJ010000292         2006 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_517039, whole genome shotgun sequence.
ACCESSION   DATCBJ010000292 DATCBJ010000000
VERSION     DATCBJ010000292.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2006)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2006)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2006
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_517039"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(91..1437)
                     /locus_tag="VII50_05895"
     CDS             complement(91..1437)
                     /locus_tag="VII50_05895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HEY5050413.1"
                     /translation="MSPRQLTEQKPGSTKTLIPGLDGAVVRRTVLPGGLRVITEAMPT
                     VRSVSFGVWVGVGSRDETPALAGSSHYLEHVLFKGTKRRDALEISAALDAVGGEMNAF
                     TSKEYTCFYARVLDNDLLLAVDVISDMMTSSVVRSSDVDSERGVILEEIAMHGDDPGD
                     VVHDAFAEALFGDTPLGRPILGTVESINALQRNAIHGYYRRRYRPENMVIAAAGNLDH
                     AKVVRLVAKAFGEMLNDDAQIDRKPSPPRVGGRPPVPRSGVSLVQRPTEQAHLVLGVP
                     ALSRVDERRFALGVLNGALGGGMSSRLFQEVREKRGLAYSVYSYAAHHAETGMFGVYA
                     GCQPAKAAEVLDICRVQLDLVARGGITAEELSRGKGQLAGALVLGLEDTGSRMSRLGK
                     AELVYGELLTVDELLARIDAVTLEQVRDVAATVLSAKQTLAVIGPFDDDAFADAVA"
     gene            complement(1434..>2006)
                     /locus_tag="VII50_05900"
     CDS             complement(1434..>2006)
                     /locus_tag="VII50_05900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009080475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HEY5050414.1"
                     /translation="REARFTILDVMAKAISGPAEMSKYAPRIITIKIPVDQIGAVIGP
                     KGKMINQIQDETGAEITIEDDGTIYIGATDGPKAEAARAQINAIANPTMPEVGERYLG
                     TVVKTTAFGAFISLVPGKDGLLHISELRKISGGKRVENVDDVVKVGDKIQVEIKEIDP
                     RGKLSLVPVVEGDAGAAGQGSVDAAVGAHE"
BASE COUNT          339 a          682 c          634 g          351 t
ORIGIN      
        1 cggaccgcgt cgcagaccgt cgcgcccatc tttccgtcag caccaagaac ggcaaccgcg
       61 gtactcatgc aactccgccc ctactgtcgc tcacgcgacg gcgtccgcga acgcatcatc
      121 atcgaacgga ccgatcacgg caagggtctg ctttgccgac agcacggtcg ccgcaacgtc
      181 gcggacctgc tcgagcgtta ccgcatcgat acgcgcgagc aactcgtcga ccgtgagcaa
      241 ctcgccatag acgagttcgg ccttcccgag ccggctcatg cgcgagccgg tgtcttcaag
      301 tccgagtacg agcgcgcccg cgagctgtcc cttgccgcgc gagagttctt cggcggtgat
      361 gccgccccgc gcgacgaggt cgagctgcac ccggcagatg tcgagcactt ctgctgcctt
      421 cgcaggctgg caaccggcgt agacgccgaa catgccggtc tcggcgtgat gtgcggcgta
      481 gctgtacacg gagtatgcga gcccgcgctt ctcgcggacc tcttggaaca agcgcgaact
      541 catgccgcca ccgagcgcgc cgttcagtac cccgagggcg aagcgtcgtt cgtcaacgcg
      601 cgacaaggca gggacgccga gcaccaggtg cgcctgctcg gtcggccgct gcaccagcga
      661 cacacccgaa cgcggcaccg gcggccgacc gccgacacga ggcggcgacg gcttgcggtc
      721 gatctgagca tcgtcgttca gcatctcgcc gaacgccttg gccaccaacc gcacgacctt
      781 ggcgtggtcg aggttgccgg cggcggcgat caccatgttc tcgggccggt acctgcgccg
      841 gtagtaaccg tgaatggcgt tgcgctgtag cgcgttgatc gattcgacgg tgccgaggat
      901 cgggcgaccc agcggcgtgt caccaaacag cgcctcggcg aacgcgtcgt gcacgacgtc
      961 gccggggtcg tccccgtgca tcgcgatctc ttcgaggatg acgccgcgtt cggagtcgac
     1021 gtccgatgag cgcacgaccg acgacgtcat catgtcggag atgacgtcga cggcgagcaa
     1081 cagatcgttg tcgaggacac gtgcgtagaa gcaggtgtat tccttcgacg tgaacgcgtt
     1141 catctctcca ccgaccgcgt cgagcgccgc cgagatctcc aacgcgtcgc ggcgcttggt
     1201 gcccttgaaa agcacgtgct cgaggtagtg cgagctcccg gcgagcgcgg gtgtctcgtc
     1261 gcgcgagccc acgccgaccc acacaccgaa cgagacggaa cgcacggttg gcattgcctc
     1321 ggtgatgacc cgaagaccac ctggcagaac ggtgcggcgg accaccgcgc cgtccaggcc
     1381 aggaatgagc gtcttggtac taccgggctt ctgttcggtc agctgacgtg gactcactcg
     1441 tgtgcgccca ccgcagcgtc gaccgaaccc tgaccagcag caccggcatc gccttcgacg
     1501 accggcacga gtgacagctt gcctctcggg tcgatctcct tgatctcgac ctggatcttg
     1561 tcgccgacct tgaccacgtc gtcgacgttc tcgacccgct tgccgccgga gatcttgcgg
     1621 agctcggaga tgtgcagcaa accgtccttg ccgggcacga gggagatgaa cgcgccgaac
     1681 gccgtggtct tcaccacggt gccgaggtag cgctcgccga cctcgggcat cgtcgggttc
     1741 gcgatcgcat tgatctgcgc gcgagccgct tccgccttcg gtccatcggt ggcgccgatg
     1801 taaatcgtgc cgtcgtcttc gatggtgatc tcagcgccgg tttcgtcctg gatctggttg
     1861 atcatcttgc ccttgggccc gatgaccgca ccgatctggt cgaccgggat cttgatcgtg
     1921 atgatccgcg gcgcgtactt cgacatctcg gcaggaccgc tgatggcctt cgccatcaca
     1981 tcgaggatcg tgaaccgagc ctcgcg
//
LOCUS       DATCBJ010000293         3254 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_437761, whole genome shotgun sequence.
ACCESSION   DATCBJ010000293 DATCBJ010000000
VERSION     DATCBJ010000293.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 3254)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3254)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3254
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_437761"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..936)
                     /gene="hrcA"
                     /locus_tag="VII50_05905"
     CDS             complement(<1..936)
                     /gene="hrcA"
                     /locus_tag="VII50_05905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HEY5050415.1"
                     /translation="MLDDRKLDVLRAIVQDFVSTNEPVGSKALVERHHLGVSPATVRN
                     DMAALEEEGYIAQPHTSAGRVPTDKGYRLFVDRLSTVKPLSSAERRAIEQFLDGALDL
                     DDVVSRTVRLLAQLTRHVAVVQYPILDRSSVRHLELIKLSDTRLMLVLITDTGRVEQR
                     QVDLPGLIDERTVGDLRARLNSRIAGRPIHDVPNDLADLAEAEPPATRGNASAVVAVL
                     LASLVERREERVVVGGASNLTRLSLDFPDSVHPVLEALEEQVVLIRLLGEASRPTEVT
                     VRIGDENPHEGLRSTSMVSATYGSGSRALASLGVLG"
     gene            1061..1555
                     /locus_tag="VII50_05910"
     CDS             1061..1555
                     /locus_tag="VII50_05910"
                     /inference="COORDINATES: protein motif:HMM:TIGR02983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SigE family RNA polymerase sigma factor"
                     /protein_id="HEY5050416.1"
                     /translation="MDDELADFVRARYPQLLRRGYLLTGSQLAAEDLVQEALARCCAA
                     WRRHPITEPDAYVHRVMVNVLISRSRLRRFREVVTADVPEVRASDVTSQHAERDAVWR
                     ALQETASRQRAVLVLRFYEDMTESEIAACLDITVGTVRSQTAKGLAKLRQLAPASIRT
                     EDPR"
     gene            complement(1641..2261)
                     /locus_tag="VII50_05915"
     CDS             complement(1641..2261)
                     /locus_tag="VII50_05915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5050417.1"
                     /translation="MTTGPVFRVPVFGSVTVIAIDVVKAGEPPSGLITSMATRSPDNS
                     GSPQLPVVAAPNESDAGSTGIRMHGAFGSVDVTAPVSSNGPESVERLKNVYVLGSASA
                     DTFGGKVVGGGLGDGATTAEATELAGDEAAGALAAGDVGGVVVGAAFAVGVAELLELT
                     AGPIGRVAGPLPASAVAAIAMPATHSAAVDATAMPRLWRVRRRIRS"
     gene            2296..3000
                     /locus_tag="VII50_05920"
     CDS             2296..3000
                     /locus_tag="VII50_05920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5050418.1"
                     /translation="MAYGGGSLWVYDVDTSNGPEAIRVSATTGHVENVASTPQLYRPI
                     MAANGDGLWLGNSIEGSPTAGTVFHVAPTAHVAATVVPNPNDAVDWMVADQGHVWAGI
                     RPSSVGGVLSVWRFDGPKATVAIHTLEPSLQAGPNSVVGDEQVGLWLTTSDPPIGDYL
                     APTDNQHLDVVKLDPNTAKPTVEAVLPPLDQLDAESQTAAGQAAFFGGSYFLLQSPSV
                     GGYTGFKDLLRFTPLP"
BASE COUNT          582 a         1082 c         1082 g          508 t
ORIGIN      
        1 accgagcaca ccaagactgg cgagtgcccg gctgcccgag ccgtacgtcg cgctcaccat
       61 cgacgtcgac cgcaagccct cgtgcgggtt ctcgtcgccg atgcgaacgg tgacctcggt
      121 gggcctgctc gcttcaccca acaggcgaat gaggacaacc tgttcttcaa gtgcttcaag
      181 caccggatgc accgagtcgg gaaagtcgag cgatagccga gtgaggttcg aggccccacc
      241 gacgacgacg cgctcctcac gacgttcgac caaggacgcg agaagcaccg cgacgacggc
      301 agacgcgttg ccacgggtgg caggaggctc ggcttcagcc agatccgcca agtcgttggg
      361 gacgtcgtgg atgggccggc cggcgatgcg cgagttcagc cgcgcacgca gatcgccgac
      421 ggttcgctcg tcaatcaagc ccgggaggtc gacctgccgc tgctcgaccc ggccggtgtc
      481 ggtgatcagc acaagcatca gccgggtgtc gctcaacttg atcagctcga ggtgtcgcac
      541 cgaggagcgg tcgaggatcg ggtactggac gaccgccacg tggcgcgtca gttgggcgag
      601 cagccggact gtcctcgaca ccacgtcgtc gaggtcgagt gcgccgtcga ggaactgctc
      661 gatcgcccgg cgctccgcgc tgctcagcgg cttgacggtc gagagccggt cgacgaagag
      721 ccggtagccc ttgtcggttg gcacccggcc ggcactggtg tgcggctggg cgatgtagcc
      781 ctcttcttcg agcgccgcca tgtcgttgcg cacggttgcc ggcgagaccc cgaggtggtg
      841 ccgttcgacc agggccttag agccgaccgg ctcgttggtc gagacaaagt cctggacgat
      901 ggcgcgcagc acgtcgagct ttcggtcgtc gagcacgcga accacctccg tcggccgtct
      961 ggcactccgt tgacccgagt gccagtgtac ggcgcgagca gcgtaggcat agaaaagact
     1021 cgtccacgcg tctaagcatg gagaagacct agggggcccg gtggacgacg agctcgccga
     1081 tttcgtgcga gcccgctatc cacagctgct ccgacggggg tatctgctga cgggcagcca
     1141 gctcgccgcc gaggacctgg tgcaggaggc gttagcgcgg tgttgcgcgg cgtggcggcg
     1201 acacccaatc accgagccgg acgcgtatgt gcatcgagtg atggtcaacg tgttgatcag
     1261 ccgctcgcgg ctgcgacgct tccgtgaagt ggtaacggcg gacgtccccg aagtcagagc
     1321 ttcagacgtc acaagccaac acgccgagcg ggacgccgtg tggcgtgcgc tgcaagaaac
     1381 cgcatctcgg caacgggccg tgctggtgct gcgcttctac gaggacatga ccgagagcga
     1441 gatcgcggcg tgcctggaca tcacggtcgg cacggtgcga agtcagaccg cgaaagggct
     1501 cgcgaagttg cggcagctcg ccccggcttc gatcaggacg gaggacccgc gatgactgac
     1561 gacatggaag aacggttgcg cgaggttctt cgtcggcgcg ccgaggtccc caaggcaccc
     1621 ggcgatcctg ttgcgtccgt tcaagagcgg atgcggcgac gcacccgcca cagccgtggc
     1681 atcgccgtcg cgtcaacggc agcactgtgc gtcgccggca tcgcgatcgc ggcaaccgca
     1741 ctagctggca acggcccggc gacgcgaccg atcggacctg cggtcagctc gagcaactcg
     1801 gcgaccccca cagcgaaggc agctccgacc acaacaccgc cgacgtcgcc tgctgcgagc
     1861 gcgccggcag cctcgtcgcc cgccagttcg gtcgcctctg ctgtcgtcgc gccgtcacca
     1921 agtccaccgc ccacaacctt gccgccgaac gtgtctgcgc ttgcgctgcc aagcacatac
     1981 acgttcttca gcctctcgac agactccggg ccgttgctgc tgacgggtgc agtcacgtcg
     2041 accgacccaa acgcgccatg catacggatg cccgtcgacc cggcgtcact ctcgttaggg
     2101 gcggccacga caggaagctg cggcgacccg gagttgtccg gcgagcgggt cgccatcgac
     2161 gtgatcaatc cggacggtgg ctcccccgct ttcaccacgt cgatcgcgat taccgtcacc
     2221 gacccaaaga ctggaacccg aaagacgggc ccagtggtca tgcgaatcag tgaggcctcg
     2281 gatacgaatc cggtcgtggc atacggaggc ggctcactgt gggtgtacga cgtggacacg
     2341 agcaacgggc cggaagccat tcgagtctcg gccacaaccg gccacgtcga gaacgtggcc
     2401 agtacgccgc agctataccg gccgatcatg gccgcgaacg gtgatgggct ctggctgggc
     2461 aattcgattg aggggtcgcc caccgcaggc accgtgtttc acgtggcgcc aactgcacac
     2521 gtggcggcca cagtcgtgcc gaacccgaac gacgccgtcg actggatggt cgccgaccag
     2581 ggccacgtct gggctggcat ccgtccgtcg agcgtgggtg gggtgttgag cgtgtggcgc
     2641 ttcgacggtc cgaaagcgac ggtcgcgatc cacacactgg aaccgagtct tcaggccggg
     2701 cctaactccg tcgtgggaga tgagcaggtc gggttgtggc tgacgacgtc cgatccgccg
     2761 atcggcgatt acctggctcc gacggacaat cagcacctcg acgtcgtgaa gctcgacccg
     2821 aacacagcga agccaacagt ggaagccgtc ttgccgccac tcgaccaact ggacgccgag
     2881 tcgcagacgg cggctggcca ggcggcgttc ttcggcggca gctacttcct tcttcagtca
     2941 ccgtcagtcg gcggctacac cggcttcaag gaccttctcc gctttactcc gttgccttag
     3001 cttccgaggc ctgcgcctcc gaggcttcgt gcctcggttt gtctcgcgcg gcaaaccagg
     3061 cgaggctcgc cgggagcagg aagacaatca gcagcgccag ccagtggcga aacgtatggc
     3121 cttcgaagcc ggtccaggcc cagtgcggcc ggtagccccc gatcaccacg cgacgacgcg
     3181 gacgagcatg gcgcccggga cgagctgcca ctcgcgggcc atggtcgcgc gcgtctcgaa
     3241 ccacaacggc aaga
//
LOCUS       DATCBJ010000294         3442 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_336184, whole genome shotgun sequence.
ACCESSION   DATCBJ010000294 DATCBJ010000000
VERSION     DATCBJ010000294.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 3442)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3442)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3442
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_336184"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..368)
                     /locus_tag="VII50_05925"
     CDS             complement(<1..368)
                     /locus_tag="VII50_05925"
                     /inference="COORDINATES: protein motif:HMM:NF022182.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjN domain-containing protein"
                     /protein_id="HEY5050419.1"
                     /translation="MSWFARTEIDAVIRATLEEREIAFDQVGESAFVVRLTGTHRLVT
                     AVRLVVGDQSLMIEAFFVRRPDENAQEFYRFLLQRNGHMYGVHYAIDAKGDVYLMGRL
                     PLLAVTPDEIDRLLGCVLTYA"
     gene            complement(376..1638)
                     /gene="mshA"
                     /locus_tag="VII50_05930"
     CDS             complement(376..1638)
                     /gene="mshA"
                     /locus_tag="VII50_05930"
                     /EC_number="2.4.1.250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019049490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010125 - mycothiol biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-inositol-3-phosphate glycosyltransferase"
                     /protein_id="HEY5050420.1"
                     /translation="MLSLHTSPVEQPGVGDAGGMNVYVMELSRRLAAQGVEVEVFTRA
                     TSGDVPPIEYIEPGFIVRNVVAGPLEPLPKADLPSQLCAFTAAVLRAEAAREPGWYDL
                     IHSHYWLSGQVGWLAKERWGVPLVHSMHTLARVKNAALAEGDAPEPSARAIGEAQVVE
                     AADRLIANTAAESRQLIDLYEADPAAVSVVPPGVDLALYRPGSRAAARGRLGLPANRD
                     VLLFVGRIQPLKAPDLLIRAVAVMIDRTPSLRDRLVAVIVGGVSGSGVAEPGHLARLA
                     EQLGVADVVRFEPPAAGDRLADFYRAASVTVVPSYSESFGLVALESQACGTPVVAARV
                     GGLVTTVSDGVSGLLVDGHEPPLYADVLTKLLSDAELRRSLATGAIAHAAGFDWDRTV
                     DGVIDVYADALRGVANRNVEGLPVAVWR"
     gene            1974..2744
                     /locus_tag="VII50_05935"
     CDS             1974..2744
                     /locus_tag="VII50_05935"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEY5050421.1"
                     /translation="MSARPPTESATTRPVAIVTGASSGIGAATARRLARDGFHVVCAA
                     RRADRISAVAAEIGGRALVLDVTDAASVTALADAVPSCSLLVNVAGGAIGAEPISAAD
                     PADWQSMYDTNVLGVLRVTQALLPALVASGAGHVIVVGSTAGRIVYEGGGGYTAAKHA
                     TAALTETLRLELNGQPVRVTEVAPGMVRTEEFSLNRNRGDAEKAAAVYAGVAEPLTAD
                     DIADCISWCATRPPHVNVDLLVVKPLAQAAQYKVYRTS"
     gene            2774..3184
                     /locus_tag="VII50_05940"
     CDS             2774..3184
                     /locus_tag="VII50_05940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5050422.1"
                     /translation="MNNGKRDLTLDQLAATQPGLDRLMAELGPRMHRLYFAGRAENWR
                     LADYFFRSVVKQLSLCAFSRPKYEEAINTYITIDCEPVRVAIKSGDGEAFESAYAAMV
                     GRANAYHDEFAKPYLHWVCPTTPPEDLDFAAGLT"
     gene            complement(3138..>3442)
                     /locus_tag="VII50_05945"
     CDS             complement(3138..>3442)
                     /locus_tag="VII50_05945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5050423.1"
                     /translation="AAIAARLLVDPRNITYYYVPVLVCLLLGQWASGRLTTLGSTLVT
                     GSWMVIAMCVFATTSGVAASWWVVVFCVGIIALAIRDGTREVRSDQLQSQDLRVAS"
BASE COUNT          652 a         1178 c         1094 g          518 t
ORIGIN      
        1 gcgtacgtca gcacgcaccc gagcagccgg tcgatctcat caggcgtcac cgcaagaagc
       61 ggcagccgcc ccatcaagta gacgtcgccc ttggcatcga tcgcgtagtg cacgccgtac
      121 atgtggccgt ttcgctggag caagaatcga tagaactcct gcgcgttctc atcgggacgc
      181 cgcacgaaaa acgcctcgat catcaggctt tggtcaccga cgaccagacg cacggccgtc
      241 acgaggcgat gggtccccgt cagtcgcaca acgaaagctg attcaccgac ctgatcgaac
      301 gcgatctcgc gttcttccaa cgtcgcccta atgacggcat cgatctcggt gcgcgcaaac
      361 cagctcacga cgcgctcacc gccatacggc gacgggcagc ccttcgacgt tgcggttcgc
      421 gacgccgcgc agcgcgtcgg catagacatc gatcacgcca tcgacagtgc gatcccagtc
      481 gaagccggcg gcgtgggcga tcgcacccgt tgccaaggag cgccgcaatt cggcgtccga
      541 taagagtttc gtcagtacgt cggcgtaaag cggcggctcg tgcccatcga cgagcaaccc
      601 cgagacacca tcgctcaccg tcgtgacgag gccgcccacc cgcgcggcga cgaccggcgt
      661 cccgcacgcc tgggattcga gcgcgacgag gccaaacgat tcgctgtagg acgggacaac
      721 ggtgacactc gcggcccggt agaagtcggc gaggcgatcg ccggcggccg gcggttcgaa
      781 gcgcaccacg tctgcgacgc cgagttgctc ggcaaggcgg gccaggtggc cgggctctgc
      841 gaccccggac ccactgacac cgccgacgat cacggccacc agtcgatcgc gcaacgacgg
      901 cgtgcgatcg atcatgacgg cgactgcacg gatcagcagg tcaggcgcct tgagcggctg
      961 gattctgccg acgaacaaga gcacgtcgcg gtttgccggc aggccgaggc ggcctcgtgc
     1021 agcggcgcgc gacccaggcc ggtacaacgc aaggtcgaca ccgggcggga cgacggacac
     1081 tgcggctgga tccgcctcgt acaggtcgat cagctgccgg gactcagctg cggtattcgc
     1141 gatcagtcgg tcggccgcct cgacgacctg cgcctctccg atggcccggg ccgacggctc
     1201 aggagcatcg ccttcggcaa gcgccgcgtt cttgacccgc gcgagtgtgt gcatgctgtg
     1261 aacgagcgga acgccccacc gctccttggc aagccagccc acctggccgg acaaccaata
     1321 gtgagagtgg atcaggtcgt accaaccagg ctccctggcc gcttcggccc gcagcactgc
     1381 ggccgtgaaa gcacacaact gcgagggcag atctgctttc ggcagcggct cgagggggcc
     1441 ggcaaccacg ttgcgaacga tgaagccggg ctcgatgtac tcgatcgggg gcacgtcacc
     1501 cgacgtcgcg cgggtgaaga cctcaacctc gacgccctgg gcggcaagcc tgcgggacag
     1561 ctccatcacg tagacgttca tcccgccggc atcgcccacg ccgggctgtt cgaccggaga
     1621 cgtgtgcaga ctcagcatcg ccagccgctt gagcggaggc cggggacccg caagtcgcga
     1681 aaccggcggt ctcgccgccg tccctctagg ggcgttcact cggggagagg cgagtcggga
     1741 ggagcgccga cgcagttttg cgacggtctg gagatcgtcc gacaagcttc ctcctccacg
     1801 ctcgctcggt catcacaacg tacgcctgag catgccgccg accgacgggc agccgccacc
     1861 tcgctgcgca acgccgaagc ggtgcggacc attcccacgc gacgtagcgg acttactagg
     1921 gcgtacctgc cctggctgac cagccgcgaa ccaggcactg ccaagattcc cttatgagcg
     1981 cacgcccacc caccgaatcg gcgacgacga ggccggtggc gatcgtcacc ggtgcgagca
     2041 gcgggattgg cgcggctacc gcgcgtcgac tcgcgcgaga cggcttccat gtcgtgtgcg
     2101 cggcccgccg agccgaccgg atttccgcgg tcgcagccga gatcggtggt cgggcgctgg
     2161 tcctggacgt cacggacgcc gcatcagtga ccgcactcgc cgatgcggtg ccctcgtgct
     2221 cgctgctcgt caacgtggcc ggcggcgcga tcggagccga gcccatctcg gcagccgacc
     2281 cggccgactg gcagtcgatg tacgacacga atgtgctggg cgtcctgcgg gtcacccagg
     2341 ccttgctgcc cgcactcgtc gcctccggag ccggtcacgt catcgtcgtc ggctcgaccg
     2401 cgggccggat tgtgtacgaa ggcggcggcg gctacaccgc cgcgaaacac gcgacggctg
     2461 cattgaccga aaccctgcgc ctcgaactga acggccaacc ggtaagggtg acggaggtcg
     2521 cccctggaat ggtacgcacg gaggagttca gcttgaaccg aaatagaggg gacgccgaga
     2581 aggccgccgc ggtctacgcc ggtgtggcag aaccactcac cgcggacgac atcgccgact
     2641 gcatcagctg gtgcgcaact cgaccgccac acgtcaacgt cgatctgctc gtcgtcaagc
     2701 ctcttgctca agcagctcaa tacaaggtgt acagaacctc atgacacagc cgccggccga
     2761 ccacgtgcac accgtgaaca acggcaagcg cgatctcaca ctggaccagt tagccgccac
     2821 ccaaccgggg ttggatcgcc tgatggctga gctcggtcca cgtatgcatc ggctgtattt
     2881 cgcaggcaga gctgagaatt ggcgactcgc ggactacttc ttccgctccg tggtgaagca
     2941 actcagcctg tgcgcgttct cacggccgaa gtatgaggag gcgatcaaca cctacatcac
     3001 gatcgactgc gagcctgtac gtgttgccat caagtctggc gatggcgaag ctttcgaatc
     3061 cgcatacgcc gcgatggtgg gacgggccaa cgcttatcac gacgagttcg caaagcctta
     3121 cctgcattgg gtgtgcccta cgacgccacc cgaagatctt gactttgcag ctggtctgac
     3181 ctgacctcgc gcgttccgtc acggatcgcg agcgcgatga tgccgacgca gaacaccacc
     3241 acccaccacg aggccgccac gccactggtg gtggcgaaga cgcacatggc gatcaccatc
     3301 cacgagcccg tcacgagggt gctgccgagg gtcgtcagtc gaccgctggc ccattgcccg
     3361 agcagcaaac acaccagcac cggcacgtag tagtacgtga tgttgcgcgg atcgaccagc
     3421 aaccgagctg cgatggcggc cg
//
LOCUS       DATCBJ010000295         5267 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_151334, whole genome shotgun sequence.
ACCESSION   DATCBJ010000295 DATCBJ010000000
VERSION     DATCBJ010000295.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 5267)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5267)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5267
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_151334"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..294)
                     /locus_tag="VII50_05950"
     CDS             complement(<1..294)
                     /locus_tag="VII50_05950"
                     /inference="COORDINATES: protein motif:HMM:NF023697.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3618 domain-containing protein"
                     /protein_id="HEY5050424.1"
                     /translation="MASDSEELLRSIERTREELANTVDTIAGRLDPKRVAKRGVDAMR
                     DGVTTVIDGARVKLHLGPEPDAVPAVAAQSSHSESAGSGTSGSASGGRAGAASA"
     gene            complement(344..1396)
                     /locus_tag="VII50_05955"
     CDS             complement(344..1396)
                     /locus_tag="VII50_05955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HEY5050425.1"
                     /translation="MSVRALSVVPGKPETAGVAEHADPSPEEGALLVQGLLVGVCGTD
                     IEIIQDGYGWVPPGQDRLVLGHESLGQVIDAPAGSGFAAGDLVAGIVRRPDPLPCEPC
                     GMGQWDFCRNGNYTERGIKERNGYAASLWRVEPEFAVKLDARLGDCGVLMEPTSVVSK
                     AWEQSEKIASRAPFDRKVALITGAGPIGLLAALLGVQRGYEVHVLDRIDSGPKPAIVS
                     DLGATYHSGDVSTVGINPDIVIECTGYGPLAFELTNVVAKDAVICLAGISGGSREVPT
                     SLNMINKQIVLENIVLFGSVNAARRHYEQAAEALAKADLAWLARLVSRKVPLTHFADA
                     LARNDDDVKVAIDLRN"
     gene            1505..1978
                     /gene="bcp"
                     /locus_tag="VII50_05960"
     CDS             1505..1978
                     /gene="bcp"
                     /locus_tag="VII50_05960"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019511616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HEY5050426.1"
                     /translation="MSSQVQLKAGDPAPEFTLPDADGQPVSLKDFRGRRVIVYFYPAA
                     MTSGCTKQACDFRDSLSQLGGAGLTVLGISPDPVAKLVRFRDEQDLTFPLLSDVDKVV
                     LDKYGAYGEKKLYGKTVIGVIRSTFVIGAAGRVEVVQYNVKATGHVAKLRRELEI"
     gene            1984..2056
                     /locus_tag="VII50_05965"
     tRNA            1984..2056
                     /locus_tag="VII50_05965"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2017..2019,aa:Leu,seq:tag)
     gene            2607..2789
                     /locus_tag="VII50_05970"
     CDS             2607..2789
                     /locus_tag="VII50_05970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5050427.1"
                     /translation="MEFADGAQTTWDPQINGNQSNADGGNNGVQAMYVDQGSDTLYLA
                     GAFLHWDGTTGLTYAS"
     gene            complement(2773..2964)
                     /locus_tag="VII50_05975"
     CDS             complement(2773..2964)
                     /locus_tag="VII50_05975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5050428.1"
                     /translation="MRRSGARVTDVAPRPRATTVKALPDAPPELAVVAVAPAVAPSAL
                     LDNNVPLLAAKVNSIRRHT"
     gene            3169..3474
                     /locus_tag="VII50_05980"
     CDS             3169..3474
                     /locus_tag="VII50_05980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5050429.1"
                     /translation="MQNNAGVTIDTFPTPLKPNTWYRIEIAISISASTATIDCAYYLD
                     DWTTPVDAVYATSAGDTGTASVAQVSFGSAATATWTGASFLDDLAVRTSTTSFIGPA"
     gene            3592..4626
                     /locus_tag="VII50_05985"
     CDS             3592..4626
                     /locus_tag="VII50_05985"
                     /inference="COORDINATES: protein motif:HMM:NF016047.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding repeat-containing protein"
                     /protein_id="HEY5050430.1"
                     /translation="MFTPLARRAMVAALAGITTIGLAAGPASATTKPVTRLAGTDRVG
                     TSIVISTQEFPTAHTAKNVILTAAYTFADAMSAGPLGKKLDAPLLLTNPTSLNSATLT
                     EIERVMPVPTTTPGTTTSGCATTPPANAVYVIGGNAAVAASVNTTLNAAGFNVVRLAG
                     TDRFGTSVAVAQCEGSPTTVFLATGLIFADALSAGPAAASKGGSVLLTAGPVLPASVG
                     TYLAGLTSPTVYAVGQAAHLAYPSATPIVGGDRFATAALVATQFFPSPTVVGVATGAN
                     FPDALAGGAYMGMLGGPVLLTTPNILHSAPSLYIAQNSATLTQAYLFGGTATLFPLVA
                     TQTSTVLNQS"
     gene            complement(4707..>5267)
                     /locus_tag="VII50_05990"
     CDS             complement(4707..>5267)
                     /locus_tag="VII50_05990"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY5050431.1"
                     /translation="VQLGGVDLRHASLAHLRERIVVVPQEGFLFRGTVLDNILIGRAD
                     ALEHEARAVVAELGLDERFGELPDGLHTDVGERGTHLSAGERQLVSLARAALTDPAVL
                     VMDEATSSIDPGTEIALERALAALTTGRTTITVAHRLTTAERADRVAVIEDGNLVELG
                     PHADLVEADGHYARLFTAWRGADAPT"
BASE COUNT          916 a         1803 c         1647 g          901 t
ORIGIN      
        1 agccgacgct gcacccgcac ggccaccgga cgccgacccc gaagttcccg aaccggctga
       61 ctctgagtgc gacgactggg ccgcaacagc gggtaccgcg tcgggctccg ggccgaggtg
      121 cagcttcacc cgtgcgccgt cgatcaccgt ggtgacaccg tcgcgcatcg cgtcgacccc
      181 acgcttcgcg acgcgcttcg ggtcgagccg gccggcgatc gtgtcgaccg tattcgcgag
      241 ctcctcgcga gtgcgttcga tcgagcgcag cagctcctcc gaatcactgg ccacagttgg
      301 cgctccttag tcgaaggggt cgaacgacac ggctggcggg gcgctagttt cgcaggtcga
      361 tggcgacttt gacatcgtcg tcattgcgcg cgagtgcgtc ggcgaaatgg gtcagcggga
      421 ccttacggct caccaaccgg gcgagccacg ccagatcggc cttcgccagc gcctcggcgg
      481 cctgctcgta gtgccgacgc gcggcgttca cgctgccgaa caacacaatg ttctcgagca
      541 cgatctgctt gttgatcatg ttcaaactgg tcggcacctc gcggctaccc ccagaaattc
      601 cggcaaggca gatgaccgcg tccttggcga ccacattggt cagctcgaac gcgagcggac
      661 catagccagt gcactcgatc acgatgtccg ggttgatgcc gacggtcgac acgtcgccgc
      721 tgtgataggt cgcaccaagg tccgacacga tcgccggctt cggaccggaa tcgatccggt
      781 cgagaacatg aacctcataa ccacgctgga cgccgaggag tgccgccagg agtccgatcg
      841 gccccgcccc cgtgatgagg gcaaccttgc ggtcaaacgg cgcccgcgac gcgatcttct
      901 cgctctgctc ccaggccttc gacaccaccg acgtcggctc catcaggacg ccgcaatcgc
      961 caagtcgcgc gtcgagcttg acggcgaatt cgggttcgac acgccaaagg cttgccgcgt
     1021 acccgttgcg ctccttgatg cctcgctcgg tgtagttgcc gttgcggcag aagtcccact
     1081 gccccatccc gcagggctcg cacggcagcg ggtcgggacg ccgcacgatg ccggccacca
     1141 agtcgccggc cgcaaagcca ctccctgcgg gcgcgtcgat gacctgcccg agagattcgt
     1201 ggccgaggac gaggcggtcc tggccgggcg gcacccatcc gtagccgtcc tggatgatct
     1261 cgatgtcggt gccgcacacg ccgaccagca ggccctgcac gagcagtgcg ccttcttcgg
     1321 gcgacggatc ggcgtgctcg gcgacacccg cggtctcggg cttgcccggc accaccgaca
     1381 aagctctgac gctcacgctt tctccccgcc catcgacgcc ccgccctcgt ctggcgaaac
     1441 tcttgggccc atcatcgcgc agccagctct cgggcctcga ggtggccggg tagcgttgcc
     1501 gaccatgagc tcgcaggtcc aactgaaggc tggcgatccg gcaccggagt tcaccttgcc
     1561 ggacgcggat ggccagccgg tctccttgaa agactttcgc ggacgccgcg tcatcgtgta
     1621 cttctaccca gccgcgatga catcgggctg cacgaagcag gcgtgcgact ttcgcgacag
     1681 cctcagccag ctcggcgggg cgggtctgac ggttctcggg atctcgcctg acccggtcgc
     1741 caagcttgtg cggtttcgcg acgagcaaga cctcacgttc ccgctactgt cggacgtcga
     1801 caaggtggtg ctcgacaagt acggcgcgta cggcgagaag aagctctacg gcaagaccgt
     1861 catcggcgtc atccgttcga cgttcgtcat cggcgccgcc gggcgcgtgg aggtcgtgca
     1921 gtacaacgtg aaggcaacgg gtcacgtcgc aaagttgcgc cgggaactcg agatctagct
     1981 ccaggggccg tagcccaatg gcagaggcac gcggtttagg tccgcgccag tgcgagttcg
     2041 actctcgccg gccctactga tcaacatcag cgctcacctg tccgcgtgcg gcgcagcttt
     2101 gcggtgaagg gtttacctac aactgacaca cactgcttgc aggtccgcct ggcgcggtgg
     2161 aggattttcg ttaggcagtg tcgaagatcg cccggggggg cggacatgca caaggtattc
     2221 gtttctcttg gtatgggtgt tctcgcggcc gcggcgatcg gggcggccgc gcccgctgcg
     2281 gcggcgatct cgaccagagt cggcgcctgc aacgcgatcg atacaccgaa agcgagaaac
     2341 gagcttcccg cgtctgacga ctgcccaagt ccatcagcta ccggcggggg cgaggtttca
     2401 tcggccgaca cccaagtcgc gccgacgaca ctcatcaatc caacaccgct ttcatttgac
     2461 cgcaccaacg gcgacatctt ggcgatcacc aagatcgacg gaacgaacga cctcgcgttt
     2521 ggcggcaact tcagtctcgt ctataccccg aacggcgtct cgcacgcagc caccaacttt
     2581 gctgtcctca gtacttcgcc acgcagttgg agttcgcgga cggcgcgcag acgacctggg
     2641 atccgcaaat caacggcaac cagagcaacg ctgacggcgg caacaacggc gttcaggcta
     2701 tgtacgtcga ccaaggcagc gacaccctct accttgccgg cgcgtttctt cactgggacg
     2761 gcacgaccgg gctcacgtat gcctcctgat tgaattcacc ttcgccgcga gcagcggcac
     2821 gttgttgtcc aacaatgccg agggcgcgac ggcgggtgcc acggccacga cggccaactc
     2881 cggtggtgca tcgggcaacg ccttcaccgt cgtcgcgcga ggccgcggcg caacgtcggt
     2941 cactcgagcg ccgctgcggc gcatggtgcg ctgggctaca gccttaccgg atcctctggg
     3001 accgccacgc tcgtcggatg gtcgggactg aacaccagct cggttgcggc ccggttctac
     3061 tacaacacgg gcagcgtcga tcccgctgca ctgatccggc tactcgacgt ccgcaatgcg
     3121 tctgcgaccg cggcgcgcat cgagctgtcg aagaacaacc agctgtttgt gcagaacaac
     3181 gccggcgtca cgatcgacac tttcccgaca ccgctcaaac cgaacacgtg gtaccgcatc
     3241 gagatagcca tttctatttc cgccagcacg gcaacgatcg actgtgcgta ctacctcgac
     3301 gactggacca cgccggtcga cgctgtgtat gcgacgagcg cgggtgacac cggcaccgcg
     3361 agcgtcgccc aggtttcttt tggctccgcc gcaaccgcga cctggacggg tgccagcttc
     3421 ctcgacgacc tcgcggtgcg aacgtcaacg acgtcgttca tcgggcccgc ctgaccactt
     3481 ccttacgttt tgttggccct acgatgaccg agtggcccgt tgaatgtgat ctggggtttc
     3541 caaacccgcc atcgggacac cgccctaccc gagtgcttga aggaggatcg tatgttcacg
     3601 ccacttgccc gacgtgccat ggtggccgcc ctcgccggca tcaccaccat cggtctcgcc
     3661 gctggacccg catcagcgac gaccaaacca gtaacacgac ttgctggtac ggaccgcgtc
     3721 ggtaccagca tcgtcatctc cacccaggag tttccgaccg ctcatacggc caagaatgtg
     3781 atcctcaccg cggcgtacac cttcgccgac gcgatgagtg caggcccgct cggcaagaag
     3841 ttggatgcgc cgctcttgct cacaaatccc acgtcactga acagcgcaac gctgaccgag
     3901 atcgagcgcg tcatgccggt cccgacaacc acccccggca cgaccaccag cggatgcgcg
     3961 acgacaccgc cggccaacgc ggtgtatgtg atcggtggca atgcggcggt cgcggcaagt
     4021 gtcaacacga cgctcaacgc ggctgggttc aacgtcgtgc ggctcgccgg caccgaccgg
     4081 ttcggaacgt ccgtcgcggt tgcacagtgt gagggatcac cgacaacggt gttcttggcc
     4141 accggtttga tcttcgccga cgcgctgtcg gccgggcctg ccgcggcgtc caaaggcgga
     4201 tcggtgctcc tgaccgccgg gccggtcttg ccagcgtcgg tcggtacata cctggccggt
     4261 ctgactagcc cgactgtgta cgcggttggt caggcggcac acctcgccta cccgagcgcg
     4321 acacccatcg tcggcggtga ccggttcgcc acagcggcat tggttgccac ccagttcttc
     4381 ccgagcccga ccgtggttgg cgttgccacc ggcgcgaact tccctgacgc gttggcaggg
     4441 ggcgcgtaca tgggcatgct cggcggtccg gtgttgttga cgacgccgaa cattctgcac
     4501 tcagctccga gcctctatat agcgcagaac agtgccactc tcacacaggc gtacctgttc
     4561 ggtgggacag cgacgctctt cccgctggtc gcgacgcaga cgagcacggt tctcaaccag
     4621 agctagcgca caagccgtgc atggccgtag ggcaaatctc ttccggaaga cgtaaaaggg
     4681 attggcccta cggccgaggc gcgcgactac gtcggggcgt cagcgccacg ccacgcagtg
     4741 aacaaccgcg cgtagtgccc gtcggcttcg acgaggtcgg cgtgcggacc gagctcgacg
     4801 aggttgccgt cctcgatcac ggcgacacgg tcggcccgct cggcggtcgt gaggcggtgc
     4861 gcgacggtga tcgtcgtgcg accggtcgtg agtgcggcca gcgctcgctc gagtgcgatc
     4921 tcagtgccgg ggtcgatgct ggacgtcgcc tcatccatga ccagtacggc tgggtccgtt
     4981 agtgccgcac gcgcgagcga caccagctgg cgctcgccag ctgacaaatg cgtaccgcgt
     5041 tcgccgacgt cggtgtgcag gccgtcgggt agctcaccga atcgctcgtc gaggccgagc
     5101 tcggcgacca ccgcccgagc ttcgtgctcc agtgcgtcgg cccggccgat caagatgttg
     5161 tcgagcaccg tgccgcggaa cagaaagccc tcctggggca cgacgacaat ccgttcccgc
     5221 aggtgcgcga ggctggcgtg ccgcaggtcg acgccgccga gctgcac
//
LOCUS       DATCBJ010000296         2136 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_491701, whole genome shotgun sequence.
ACCESSION   DATCBJ010000296 DATCBJ010000000
VERSION     DATCBJ010000296.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2136)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2136)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2136
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_491701"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(299..1366)
                     /gene="purM"
                     /locus_tag="VII50_05995"
     CDS             complement(299..1366)
                     /gene="purM"
                     /locus_tag="VII50_05995"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HEY5050432.1"
                     /translation="MTDPDSDPTTYAAAGVDIEAGDRAVELMKASVRKASRPEVVGGL
                     GGFAGLFRFDAARYRQPLLATSTDGVGTKVAIAQAMDKHDTIGIDLVGMVVDDLVVCG
                     AEPLFMTDYIACGKVVPERIAAIVSGIAEGCRQAGCALIGGETAEHPGLLDADEYDLA
                     GAGTGVVEEDAVLGAHRVRDDDVVIAMASSGLHSNGYSLTRHVLLSRAGLALDAHVDE
                     LGRTLGEELLVPTRIYSLDCLALINAVDVHAFSHVTGGGLAANLDRVLPAGVDIEIDR
                     TTWAAAPIFALIQHLGDVATPELERTLNMGVGMIALVAAADADRAFDVLTRRGVHAWV
                     AGRARVGQGPGTTTLVGTHRN"
     gene            complement(1368..>2136)
                     /locus_tag="VII50_06000"
     CDS             complement(1368..>2136)
                     /locus_tag="VII50_06000"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HEY5050433.1"
                     /translation="VQFAEPDPKFCVFEFVYLARPDSSLDGRSVHATRVEVGRQLARE
                     APAIADLVMPTPDSGTPAAIGYAEESGIPYAAGLVKNAYVGRTFIQPSQTLRQLGIRL
                     KLNPLRDVIRGKRLVVVDDSIVRGNTQRAVVRMLREAGATEVHVRISSPPVKWPCFYG
                     IDFATRAELIASGLTVEEIRTSIGADSLSYVSLDGLVAATNRPKQNLCRACFDGQYPT
                     ALPDPRFLGKDLLEITPVEALMATVPSVGGHTALEHP"
BASE COUNT          379 a          704 c          706 g          347 t
ORIGIN      
        1 gagaagcgca ttcgcgaggt aggcagcctc tgaggagcgc gcctgctcag tgtcgggagt
       61 cggcgctgcc ggcgaagcta gcagcaacgg ggctaccgac ccggaacccg aagtcgctga
      121 caccgcagca ctaagttgcg acccaggact ggatgctggc gtgccagacg acccgacagt
      181 tctgctcgcg cacccagcca gcgtcatcgt cattgctgcg attgctattg ccgcgccacg
      241 agccgtcatg ccacttcgac gtcgcgcggc ggtcatcggt tgcctcagcg ttcgcatgtc
      301 agtttcggtg cgtaccgacg agggttgtcg ttcccggccc ctgacccacg cgggctcgtc
      361 cggcgaccca cgcgtgcacc ccgcggcggg tcaacacatc gaacgcgcgg tcggcgtccg
      421 ctgctgcgac cagcgcgatc atgccgacgc ccatgttcag cgtgcgttcc agctccgggg
      481 tggcgacgtc gccgaggtgt tggatcagag cgaagatcgg tgcggctgcc cacgtcgtcc
      541 ggtcgatttc gatgtcgacc cccgctggca acacgcggtc gaggttcgcg gccaacccgc
      601 cgcccgtgac atgcgagaac gcgtggacgt cgaccgcgtt gatcaaggcg aggcaatcga
      661 ggctgtagat acgggtcggt acgagcagtt cctcaccgag cgtgcggccg agttcgtcga
      721 cgtgcgcatc gagggccaac ccggcgcgcg acagcagcac atggcgggtc agtgagtacc
      781 cgttggaatg cagcccactc gatgccatcg caatcacgac gtcgtcgtcg cggactcgat
      841 gcgcgccgag caccgcatcc tcctcgacaa cacctgtgcc agcgcccgca aggtcgtact
      901 catcggcgtc gagcaatccg ggatgctccg ccgtctcacc gccaatcagt gcacacccgg
      961 cttgccggca gccctcggcg atgccgctaa cgatcgccgc aatgcgctct ggcacgactt
     1021 tgccgcaggc gatgtagtcg gtcatgaaga gcggctccgc accgcacaca acgaggtcgt
     1081 cgacaaccat gccgaccaga tcgatcccga tggtgtcgtg cttgtccatc gcctgtgcga
     1141 tagcaacctt cgtgccaacg ccgtccgtgg atgtcgccaa caacggttgg cggtagcgcg
     1201 ctgcgtcgaa acggaaaaga ccagcgaacc cgccgagacc gccgaccacc tcgggtcgcg
     1261 atgccttgcg gaccgatgct ttcatcaact cgaccgcgcg gtcaccagcc tcgatgtcga
     1321 cacccgcggc ggcgtaggtg gtggggtcgg agtcggggtc ggtcacgtca cgggtgttcg
     1381 agggcggtgt ggccgccgac cgacggaaca gtggccatca aggcctcgac cggcgtgatc
     1441 tcgagcaggt ccttgccgag gaatcgcggg tcgggcagcg ccgtcgggta ctgcccatcg
     1501 aagcaggcac gacaaagatt ttgcttcggg cggttggtcg ccgccaccag cccgtcgagg
     1561 gagacgtacg acagcgaatc ggcaccgatc gacgtccgga tctcttcgac ggtcaagccg
     1621 ctcgcgatga gttcggccct cgtcgcgaag tcgatgccgt agaagcacgg ccacttcacc
     1681 ggcggcgacg agatgcgcac atgcacttcg gtggcgcccg cttcgcgcaa catccggacg
     1741 acggcgcgct gtgtattgcc acgcacgatc gagtcgtcga cgacgacgag acgcttgccg
     1801 cgaatgacgt cgcgcagtgg attgagcttc agccggatgc caagctgacg caacgtctgc
     1861 gacggctgga tgaacgtgcg gccaacgtac gcgttcttga caaggcctgc ggcataaggg
     1921 attccggact cctccgcata gccgatggcg gccggcgtac cggagtcggg tgtgggcatc
     1981 acgaggtcgg caatggccgg tgcctcgcgt gcgagttggc ggccgacctc gacacgggtc
     2041 gcgtgcacac tgcggccgtc gaggctcgag tcgggacgcg cgagatagac gaactcgaag
     2101 acgcagaact tcgggtctgg ctcagcgaac tgcacc
//
LOCUS       DATCBJ010000297         2323 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_224372, whole genome shotgun sequence.
ACCESSION   DATCBJ010000297 DATCBJ010000000
VERSION     DATCBJ010000297.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2323)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2323)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2323
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_224372"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1472
                     /locus_tag="VII50_06005"
     CDS             <1..1472
                     /locus_tag="VII50_06005"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009080475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HEY5050434.1"
                     /translation="TADFPVFLEYQDDVYAAVSAVVSTELAQAMTIVSKADREAELDR
                     VKALATEKVAGQFEGREKEIGGAFRALTKKVVRERVIRDKVRIDGRGLTDIRPLSAEI
                     DVLPRVHGSALFERGETQILGVTTLNMLRMEQQLDTLAPETRKRYMHNYNFPPYSTGE
                     TGRVGSPKRREIGHGALAERALVPVLPSREDFPYAIRQVSEALGSNGSTSMGSVCAST
                     MALFASGVPLRAPVAGIAMGLISDTVDGQTQYVTLTDILGAEDAYGDMDFKVAGTREF
                     VTALQLDTKLDGIPASVLAGALTQAREARFTILDVMAKAISGPAEMSKYAPRIITIKI
                     PVDQIGAVIGPKGKMINQIQDETGAEITIEDDGTIYIGATDGPKAEAARAQINAIANP
                     TMPEVGERYLGTVVKTTAFGAFISLVPGKDGLLHISELRKISGGKRVENVDDVVKVGD
                     KIQVEIKEIDPRGKLSLVPVVEGDGAGKSPADAAVGAHE"
     gene            1469..>2323
                     /locus_tag="VII50_06010"
     CDS             1469..>2323
                     /locus_tag="VII50_06010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HEY5050435.1"
                     /translation="MSPRQLTEQKPGSTKTLIPGADGAVVRRTVLPGGLRVITEAMPT
                     VRSVSFGVWVGVGSRDETPALAGSSHYLEHVLFKGTKRRDALEISAALDAVGGEMNAF
                     TSKEYTCFYARVLDNDLPLAVDVISDMMTSSVVRSADVDSERGVILEEIAMHEDDPGD
                     VVHDAFAEALFGDTPLGRPILGTVASINALQRSAIHGYYRRRYRPENMVIAAAGNLDH
                     AKVVRLVAKAFAEMLNDSAQIDRRPSPPRVGGRPPVCRSGVSVVQRPTEQAHLVLGVP
                     ALSRVDERR"
BASE COUNT          418 a          743 c          782 g          380 t
ORIGIN      
        1 cgaccgccga cttcccggtc ttcctcgagt accaggacga tgtctacgcg gcggtgtcgg
       61 cggtagtcag caccgagctc gcgcaggcga tgacgatcgt gagcaaggcc gatcgcgagg
      121 ccgagctcga ccgcgtgaag gcgctggcga cggagaaggt cgccggtcag ttcgagggtc
      181 gcgagaagga gatcggcggc gcgttccggg cgctgaccaa gaaggtcgtc cgcgagcggg
      241 tcatccgcga caaggtgcga atcgacggcc gcggactcac ggacatccgg ccgctgtcgg
      301 ccgagatcga cgtgttgccg cgagtgcacg ggtcggcgct gttcgagcgt ggcgagacgc
      361 agatcctcgg cgtcacgacg ctgaacatgc tgcgcatgga gcagcagctc gacacgctcg
      421 ccccggagac tcgcaagcgc tatatgcata actacaactt cccgccgtac tcgaccggtg
      481 agaccggccg ggtcggttcg ccgaagcggc gcgagatcgg gcacggcgca cttgccgagc
      541 gcgcgctcgt cccggtgttg ccgtcgcgtg aggacttccc ttacgcgatc cggcaggtgt
      601 cggaggcgtt gggttccaac ggctcgacgt cgatgggttc tgtgtgtgcc tcgacgatgg
      661 cgttgttcgc ctccggtgtg ccactgcgcg cgccggtcgc cgggatcgcg atgggactga
      721 tctccgacac cgtcgacggg cagacgcaat acgtgaccct gaccgacatc ctcggtgccg
      781 aggacgccta cggcgacatg gacttcaagg tcgccggcac ccgggaattc gtgacggcgc
      841 tgcagctcga caccaagctc gacggcatcc cggcgtccgt tctcgcgggt gcactgaccc
      901 aggctcgcga ggcccggttc acgatcctcg acgtcatggc gaaggcgatc agcggcccgg
      961 ccgagatgtc gaagtacgca ccgcggatca tcaccatcaa gatcccggtc gaccagatcg
     1021 gtgcggtcat cgggcccaag ggcaagatga tcaaccagat ccaggacgaa accggcgccg
     1081 agatcaccat cgaggacgac ggcacgatct acatcggcgc gaccgacgga ccgaaggcag
     1141 aagcggcccg cgcacagatc aatgcaatcg cgaacccgac tatgccggag gtcggcgagc
     1201 gctacctcgg aaccgtggtg aagactacgg cgttcggcgc gttcatctcc ctcgtccccg
     1261 gcaaggatgg tttgctgcac atctccgagc tccgcaagat ctccggcggt aagcgggtcg
     1321 aaaacgtcga cgacgtggtc aaggtcggcg acaagatcca ggtcgagatc aaggagatcg
     1381 acccgagagg caagctgtca ctcgtcccgg tcgtcgaagg tgacggtgct ggcaagagtc
     1441 cggccgatgc tgcggtgggc gcacacgagt gagtccacgt cagctgaccg aacagaagcc
     1501 cggtagtacc aagacgctca ttcccggcgc ggacggcgcg gtggtccgcc gtaccgtcct
     1561 gccgggaggt cttcgggtca tcaccgaggc gatgcccacg gtgcgctcgg tctcgttcgg
     1621 tgtctgggtt ggcgttggct cgcgcgacga gacgccagcg cttgcgggaa gttcgcacta
     1681 cctcgagcac gtgttgttca agggcaccaa gcgccgcgat gcgctggaga tctcggcggc
     1741 gctcgacgcg gtgggcggtg agatgaacgc gttcacctcg aaggaataca cctgctttta
     1801 cgcccgcgta ctcgacaacg atttgccgct cgccgtcgac gtcatctccg acatgatgac
     1861 gtcgtcggtc gtgcgctcgg cggacgtcga ttccgaacgc ggtgtcatcc tcgaagagat
     1921 cgccatgcac gaggacgatc ccggtgacgt cgtgcacgac gcgtttgcag aggccctgtt
     1981 cggtgacaca ccactagggc gcccgatcct cggcaccgtc gcatccatca acgcactcca
     2041 gcgcagcgcc attcacggtt actaccggcg caggtatcgg cccgagaaca tggtcatcgc
     2101 ggccgccggc aacctcgacc acgccaaggt cgttcggctg gtggcgaagg cgttcgccga
     2161 gatgctgaac gacagtgccc agatcgatcg ccggccgtcg ccgcctcgcg tcggtgggcg
     2221 accaccggtg tgtcgctcag gtgtttcggt ggtgcaacgg ccaaccgagc aggcacatct
     2281 ggttctcggc gtgcctgcgt tatcgcgtgt cgacgaaaga cgg
//
LOCUS       DATCBJ010000298         3026 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_516848, whole genome shotgun sequence.
ACCESSION   DATCBJ010000298 DATCBJ010000000
VERSION     DATCBJ010000298.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 3026)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3026)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3026
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_516848"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(10..654)
                     /gene="rplD"
                     /locus_tag="VII50_06015"
     CDS             complement(10..654)
                     /gene="rplD"
                     /locus_tag="VII50_06015"
                     /inference="COORDINATES: protein motif:HMM:NF012782.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HEY5050436.1"
                     /translation="MAKSAPKLAGSGGDVVLDAVAFEASFNMPLVHETVRAELNARRS
                     GTASTKTHGQVSGGGAKPWRQKGTGRARAGSSRSPIWTGGGTVFGPQPRHYTVKVNRK
                     ARKAALRSALSVHAERESLAVFDATTFDGPSTKQAADLLGDWGADTPTLVLLTATEAN
                     AGLSFRNLVSADVMPAVDAGVADIIRAASLLVSEAAMSELVARATKGASEEETD"
     gene            complement(647..1264)
                     /gene="rplC"
                     /locus_tag="VII50_06020"
     CDS             complement(647..1264)
                     /gene="rplC"
                     /locus_tag="VII50_06020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018847505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HEY5050437.1"
                     /translation="MAAILATKLGMTQRFLDDGRVERVTVLEAGPCPVTAIRTKERDG
                     YEAVQLAFGACKEKVLTRAQLGHLKKADASAHRHLAEFRDEAGELLVGETVTVGAFEV
                     GQKVKISGKSKGKGFAGTIKRHNFASGPKSHGSHNVRAPGSIGASATPSRVMKGIRGP
                     GRMGGGRITQRGLTIVEIMAARNLLMVRGAVPGPKGSTVEVRTDG"
     gene            complement(1269..1586)
                     /gene="rpsJ"
                     /locus_tag="VII50_06025"
     CDS             complement(1269..1586)
                     /gene="rpsJ"
                     /locus_tag="VII50_06025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012084982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HEY5050438.1"
                     /translation="MAQNKIRIRLKSYDHTAIETAAKEIVETATRTGATVSGPVPLPT
                     EKNVYCVIRSPFKDKDSREHFEIRTHKRLIDIHQPTPKTVDSLQRLDHLPAGVDIEIR
                     LAS"
     gene            complement(1714..2904)
                     /gene="tuf"
                     /locus_tag="VII50_06030"
     CDS             complement(1714..2904)
                     /gene="tuf"
                     /locus_tag="VII50_06030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005055596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HEY5050439.1"
                     /translation="MAKAKFERDKPHVNVGTIGHIDHGKTTLTAAITKVLHDKYGDGT
                     EVRSFDSIDNAPEEKARGITIATSHVEYETDKRHYAHVDCPGHADYVKNMITGAAQMD
                     GAILVVSAADGAMPQTREHILLARQVGVPYIVVFLNKADMVDDPELLELVEVEIRDLL
                     SEYEFPGDDIPFVIGSALKALEGDADYEQKILDLAEALDTYIPEPVRDLDKPFLMPVE
                     DVFSITGRGTVATGRIESGIIKTGETVEIVGIKPTTTTVVTGVEMFKKNLDQGQAGDN
                     AGCLLRGTKREDIERGQVLCKPGSITPHTKFKAEVYVLKKEEGGRHTPFFTGYRPQFY
                     FRTTDVTGVANLPDGTEMVMPGDNVQMQIELIQPIAMDQGLRFAIREGGRTVGSGVVT
                     EVIQ"
BASE COUNT          458 a         1000 c         1009 g          559 t
ORIGIN      
        1 tggcttccat cagtcggtct cctcctcgct ggcgcccttg gtcgcgcggg ccaccagttc
       61 cgacattgcc gcctcggaga ccagcagcga cgcggcgcgg atgatgtccg ccacgccggc
      121 atcgacggcc ggcatcacgt cggcgctgac gaggttgcga aaggagaggc cggcattggc
      181 ctcggtcgcg gtaagcagca cgagcgtcgg cgtatcggcg ccccagtcac ccagcagatc
      241 agccgcttgc ttggtcgagg gaccatcgaa ggtggtcgcg tcgaacacgg ccagcgactc
      301 gcgctcggca tgaaccgaga gggccgagcg cagcgccgcc ttgcgggcct tgcgattgac
      361 cttgaccgtg tagtggcgcg gctgaggacc gaagacggtg ccgcccccgg tccagatcgg
      421 cgagcgcgag gacccggcgc gagcgcgacc ggtgcccttc tggcgccacg gcttggctcc
      481 gcctccggaa acctgaccat gggtcttggt cgacgccgtg cccgaacggc gcgcgttgag
      541 ctcggcgcgg acggtctcat gtacgagcgg catgttgaag ctggcctcga acgccacggc
      601 gtccaggacg acgtctccgc cgctaccggc cagtttggga gcgctcttag ccatcggtgc
      661 gcacctcgac ggtggagccc ttcgggcccg ggacggcgcc acgcaccatc agcaggttgc
      721 gggcggccat gatctcgacg atcgtcagcc cgcgttgggt gatccgcccg ccgcccatgc
      781 ggccggggcc gcggatgccc ttcatgaccc gcgacggtgt cgccgacgcg ccgatcgagc
      841 ccggggcgcg cacgttgtgc gagccgtggg acttgggccc ggaggcgaag ttgtggcgct
      901 tgatcgtgcc ggcgaagccc ttgcccttcg acttgcccga gatcttcacc ttctggccga
      961 cctcgaacgc cccgacggta accgtctcac cgacgagcag ctcgccggcc tcgtcgcgga
     1021 actcggcgag gtggcggtgc gccgacgcgt cggccttctt gaggtggccg agctgcgccc
     1081 gggtgaggac cttctccttg cacgcgccga acgcgagctg cacggcctca tagccgtcgc
     1141 gctctttggt gcgaatggcg gtcaccggac acggaccggc ctcgagcacg gtgacgcgct
     1201 cgacgcggcc gtcatcgagg aaccgctggg tcatcccgag cttggtggcg aggatcgcgg
     1261 ccatcggatc aggacgcgag gcggatctcg atatccacgc cggccggaag gtgatcgagg
     1321 cgctgaagcg agtcgaccgt cttgggcgtc ggctgatgga tgtcgatcag tcgtttgtgg
     1381 gtgcggatct cgaagtgctc acgggaatcc ttgtccttaa acggcgaccg gatcacgcag
     1441 tacacgttct tctccgtcgg cagcggcacg ggaccggaaa cggtcgcgcc ggtgcgcgtc
     1501 gccgtctcga cgatctcctt ggctgccgtc tcgatcgccg tgtggtcgta tgacttgagg
     1561 cgaatgcgga tcttgttttg ggcgatggtt gccacgaaaa ggccggttct ctcaaatcaa
     1621 cgggcgagcc ccgagcccgg ggcccgccct gatggtcgta tcgaacttcg caaaaaaagg
     1681 gcggcgggcc gtcaggccca gccgccctcc caactactga atgacttcgg tgacgacccc
     1741 ggagccgacc gtgcggccgc cctcccggat cgcgaaccgc agaccctggt ccatggcgat
     1801 cggctggatc agctcgatct gcatctggac gttgtccccg ggcatcacca tttccgtgcc
     1861 gtcgggcagg ttggccacgc cggtgacgtc ggtcgtgcgg aagtagaact gcggccggta
     1921 gccggtgaag aacggggtgt gacgcccgcc ctcctccttc ttgaggacgt agacctcggc
     1981 cttgaacttg gtgtgcggcg tgatcgagcc cggcttgcac agcacctggc cgcgctcgat
     2041 gtcctcgcgc ttggtaccgc gcaacagaca gccggcgttg tctcccgcct gaccctgatc
     2101 gaggttcttc ttgaacatct cgacgccggt gaccacggtc gtggtcgtcg gcttgatgcc
     2161 gacgatctcg acggtctcac ccgtcttgat gatccctgac tcgatccgtc cggtcgccac
     2221 cgtgccgcgg ccggtgatcg agaagacatc ctcgaccggc atcaggaacg gcttgtccag
     2281 gtcacggaca ggctccggga tgtaggtgtc cagcgcctcg gcgagatcca ggatcttctg
     2341 ctcgtaatcc gcgtcgccct ccagcgcctt gagcgcggag ccgatcacga atgggatgtc
     2401 gtcaccgggg aactcgtact ccgacagcag gtctcggatc tcgacctcga cgagctcaag
     2461 cagctcgggg tcgtcgacca tgtcggcctt gttgaggaag acgacgatgt agggcacgcc
     2521 gacctgccgg gcgagcagga tgtgctcacg cgtctgcggc atcgcgccgt ccgccgccga
     2581 gaccaccagg atcgccccgt ccatctgggc ggcgccggtg atcatgttct tcacgtaatc
     2641 ggcgtgaccc gggcagtcca catgggcgta gtgccgcttg tcggtctcgt actcgacatg
     2701 ggaggtcgcg atcgtgatgc cgcgggcctt ctcctccggt gcgttgtcga tcgagtcgaa
     2761 cgaacgcacc tcggtgccgt cgccgtactt gtcgtggagc accttggtga tggccgcggt
     2821 cagcgtcgtc ttgccatggt cgatgtgacc gatggtgcca acgttgacat ggggcttgtc
     2881 gcgctcgaac ttggctttcg ccacttggga ttgctccttg tactcagtgg atgtcgttcg
     2941 tcctgtagac gaacctcaag aatctatcgg acttccgtcc ggccacgctg ctaggccgcc
     3001 atcttctcgc cgacgacctt ctcagc
//
LOCUS       DATCBJ010000299         2123 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_313486, whole genome shotgun sequence.
ACCESSION   DATCBJ010000299 DATCBJ010000000
VERSION     DATCBJ010000299.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2123)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2123)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2123
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_313486"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1465
                     /locus_tag="VII50_06035"
     CDS             <1..1465
                     /locus_tag="VII50_06035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011718889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ferric reductase-like transmembrane
                     domain-containing protein"
                     /protein_id="HEY5050440.1"
                     /translation="CSPVRCSARGVLAMSADGTVPETLRAPVPSLSAAPARGRGSASP
                     ARRGPTPLSARPNVVLLAVALGAFAVLLLWWRGTPYVHGMGDWLTNGGRITGLLAGYT
                     IGIVLLFMARIPAFERGVGSDRLARWHAIGGRYTVSLVVCHVMLIVWGYAVTAHTNPV
                     HQTISLVSSYPDMAMATVGAGLLVFVGAVSARAARRRLRYETWYYLHLYTYIAVMLSF
                     SHQLATGAQFISDPLARVTWRSFYLVVGALLVWHRILSPIRSAWRHRMRVVGLTTEAP
                     GVISVYISGDHLDELDAQPGQFFRWRFLTREHWWAANPYSLSAPPRPRLLRITVKALG
                     DHSSDLAGVRVGTRILAEGPYGGFTAARRRRRKVLLIAAGVGITPIRALFETLPGAPG
                     DITLLYRVSSPESALFHDELEAIAHNRGHRLYLLSGPRGGSGDPLSAGRLVKAIPDVR
                     HHDVYLCGPPAMTEATVRTLRRCGVSRRRIHRESFEL"
     gene            1482..2123
                     /locus_tag="VII50_06040"
     CDS             1482..2123
                     /locus_tag="VII50_06040"
                     /inference="COORDINATES: protein motif:HMM:NF016121.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HEY5050441.1"
                     /translation="MKRAVLSTLGTVTAIVVLLGFKSAPVRVARPAFVSPLSSNEPPV
                     LGSPATSPNVASGAASSAATSIASDSPTTGPASSTTQPAAPTTRAGATHAPSTAPNKT
                     PTQVPTKAPTRSAAPPTKAASGTFLGASVQTRYGPVQIKITYAGGRVTGVVAVQLPSG
                     RSRDAEINNYAVPILESETLAAQGANINSVSGATYTSDGYVQSLQSALDAAHR"
BASE COUNT          350 a          725 c          647 g          401 t
ORIGIN      
        1 gtgctcgccg gtgcgctgct cggcgcgtgg ggtcttggcc atgtcggcgg atggcaccgt
       61 gcctgagacg ctacgggcgc cagttccgtc gctgagcgcc gcgcccgccc gagggcgcgg
      121 cagcgcgtcg cccgcgcgtc gtggcccaac gcccctcagc gctcgaccca atgtcgtgct
      181 ccttgccgtt gcactcggtg ccttcgcggt actgcttctc tggtggcgcg gcacaccgta
      241 cgtgcacggt atgggcgact ggctcaccaa cggtggcagg atcaccggcc tgctcgctgg
      301 ctacacaatc ggcatcgtcc ttctcttcat ggcgcggatt ccggctttcg aacgcggcgt
      361 cggttctgat cgacttgccc gttggcatgc catcggcggc cggtacaccg tcagtctcgt
      421 cgtctgccac gtcatgctca tcgtgtgggg ctatgccgtc acagcgcata ccaatccggt
      481 tcatcagacc atcagccttg tcagcagtta ccccgacatg gcgatggcca ccgtaggagc
      541 tggcctgctg gtttttgtcg gagcagtatc tgcgagagca gcacggcggc gattgcggta
      601 cgagacctgg tactacctgc acctgtacac gtacatcgcg gtgatgttgt cgttcagtca
      661 tcagcttgcg accggcgcac agttcatctc tgatccgctt gcccgcgtga catggagaag
      721 cttctacctc gttgtcggcg cactcttggt ctggcatcgg atcttgagcc ccattcgaag
      781 tgcctggcgc caccgcatgc gagttgtggg tctcacgacc gaagcaccag gcgtaatctc
      841 ggtctacatc tccggcgatc atctcgacga actcgatgcc caaccgggcc agttctttcg
      901 ctggcgattt ctcacccgtg agcactggtg ggcggccaac ccatactcct tgtctgcacc
      961 tccgaggcct aggcttctcc ggataacggt gaaagcgctg ggcgaccaca gcagcgatct
     1021 cgccggcgtc cgcgtcggaa cgagaattct cgccgagggt ccgtacggcg ggttcactgc
     1081 agcacgcagg aggcggcgga aggtcttgct gatcgctgcc ggcgttggca ttacgccaat
     1141 ccgggcgcta ttcgagactc ttccaggtgc gccgggagat atcaccttgc tgtaccgggt
     1201 cagctcgcca gaaagcgcgc tgttccacga cgaactcgaa gccatcgccc acaaccgagg
     1261 tcaccggctc tatctcctca gcgggccgcg tggcggaagc ggcgacccgc tctcggccgg
     1321 tcggctggtc aaggccatac ctgacgtgcg gcatcacgac gtgtatctgt gcggaccacc
     1381 cgcgatgacc gaggcaacgg tacgcacgct tcggcgttgc ggtgtctcgc ggcgccgcat
     1441 tcaccgcgag tctttcgagc tgtgacgcgc gggtcgctgt catgaagcgc gcagtccttt
     1501 cgacgcttgg caccgtcaca gccatcgttg tgttgctggg cttcaagtcc gcgccggtgc
     1561 gagttgcccg ccctgcgttc gtttcaccac tcagttcgaa cgaaccgcca gtcctcggtt
     1621 caccggcgac ctcaccgaac gtggcatcgg gcgcggcttc gagcgccgct acgtcgatcg
     1681 catcggattc accgacgacc ggcccagcgt catcgacgac ccaaccggct gcaccgacga
     1741 cgcgagctgg cgcaacgcac gcaccctcca ccgcgccgaa caagacgccg acgcaggtcc
     1801 cgacgaaagc gcctactcga tcagcggcgc caccgaccaa ggccgcaagt ggcaccttcc
     1861 tcggagcgag cgtgcagaca cgctacggcc cggttcagat caagatcacc tacgccggcg
     1921 gtcgcgtgac aggcgtggtc gcggtacagc ttccgtcggg acgttctcgg gacgccgaga
     1981 taaacaacta cgcggtgcct atcctcgaga gcgagacgct ggcggcacaa ggcgcgaaca
     2041 ttaactccgt gtccggcgcc acgtacacga gcgacggcta cgtgcagtcg cttcaatccg
     2101 ctctcgacgc cgcccaccgc taa
//
LOCUS       DATCBJ010000300         2001 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_232982, whole genome shotgun sequence.
ACCESSION   DATCBJ010000300 DATCBJ010000000
VERSION     DATCBJ010000300.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2001)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2001
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_232982"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..242
                     /locus_tag="VII50_06045"
     CDS             <1..242
                     /locus_tag="VII50_06045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5050442.1"
                     /translation="QGDVVNQTRFAGRWESTSRSAPHTPAGTFARLWVLPGHDAVAIG
                     LARDFQARYCGPLLQVNELVGDTVSDPSVSLLTRV"
     gene            complement(761..1129)
                     /locus_tag="VII50_06050"
     CDS             complement(761..1129)
                     /locus_tag="VII50_06050"
                     /inference="COORDINATES: protein motif:HMM:NF025210.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HEY5050443.1"
                     /translation="MSVAGGQTLRVLDGSYVLDRPAEGTDVDLRGDVLAVVFGPDGST
                     VMRRDDAGEDGWSALWNGDEAHDVQATGMLSAIVAPLAAAGVPVWVVSSFDGDLVLVP
                     TDRVDGACDVLQAAGHQVRR"
     gene            complement(1129..1707)
                     /locus_tag="VII50_06055"
     CDS             complement(1129..1707)
                     /locus_tag="VII50_06055"
                     /inference="COORDINATES: protein motif:HMM:NF013989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HEY5050444.1"
                     /translation="MARLIYAAIASLDGYVEDTAGAFDWSAPDDEVLAFVNDLERPIG
                     THLYGRRMYETMTYWETASIDAGQTEPERDFARNWRAAEKIVYSRTLPAPTTVRTRLE
                     RSFDPADVRRLKQSSTSDLSIGGADLAGQALAAGLVDECQVFLGAIAVGGGKPVFPLG
                     VRLRLELLDEHRFSGGTVCLRYAVFPAEKATI"
     gene            1746..>2001
                     /locus_tag="VII50_06060"
     CDS             1746..>2001
                     /locus_tag="VII50_06060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5050445.1"
                     /translation="MRVGDAGSNGRPVALASLGEVLELPCVHARQPILAVRGRWADPR
                     VAGNSGVTPGVHVRAILVDPCAYLISSADGPVARDEDIDVA"
BASE COUNT          319 a          721 c          639 g          322 t
ORIGIN      
        1 gccaaggaga cgtcgtgaat cagacgcgat tcgccggacg ttgggaatcg accagccgca
       61 gtgcccctca taccccggcc ggtaccttcg ctcggctatg ggtcctccct ggacacgatg
      121 ccgttgccat tgggctggcc cgtgacttcc aagccaggta ctgtggcccg cttctccagg
      181 tcaacgagct ggttggcgac accgtatccg accccagcgt ctccttgctg acccgagtgt
      241 aggtgaccca ggtcgccgta tgtgctattg caaccagaag cccgtgtgta agtcataacc
      301 acacgtatcg gacggctccg caccgcgcca aacggccgac cgcagctata tcgccggata
      361 tttacatctc tccgagggcg gacgccattg ccgtatcgag cacagatcag cccgttcggg
      421 gcggccctca gcgctacccg ctaccggtgg agtcgtgcgg cacccgccgc gcttgcaacg
      481 agcgaccggg cggacacctc agcctgttcc ctcggccgtc agcactctgt agcgaaggtg
      541 gctcgcgctg ctgcaccgcg cccgtgtcgt cgcgatcgag ctcgtcgatc tccaggccga
      601 atgccacggt caggaagccg gcgccgggtc gacgttccac gtcagatccg gatacacgtc
      661 catgacgacg gcagaccagc cggactcgtg ccgtagccgg gcgccgaccg ccgttcggcg
      721 ctgaccatgc actacgccat gcagtgcccg acattcccgg ttagcgccgg acttggtggc
      781 cggccgcctg cagcacgtca caggccccat ccacccgatc ggtcgggacc aggacgaggt
      841 cgccgtcgaa actcgaaacc acccacaccg gcacgccagc ggccgcgagc ggcgccacga
      901 tcgcgctcag catcccggtc gcctgcacgt catgggcctc gtcgccattc cacagcgccg
      961 accatccatc ttcgcctgcg tcgtcgcgcc gcatcactgt cgatccgtcc ggaccgaaca
     1021 cgacggcgag cacatccccg cgcaggtcaa cgtcggtgcc ctctgccgga cgatcaagaa
     1081 cgtacgaacc gtcgagcacc cgcaacgtct gaccgccggc gacggacatc agatcgttgc
     1141 tttctcggcc gggaacaccg cgtaacgaag gcagaccgtg ccgccgctaa agcggtgctc
     1201 gtcgagcagc tcgagccgta ggcggacacc gagcgggaag accggcttgc cgccgccgac
     1261 cgcgatcgcg ccgaggaaga cctgacactc gtcgactagc ccggcggcca gcgcctggcc
     1321 ggcgaggtcg gcgccgccga tcgagaggtc gctcgtcgac gactgcttga gccgccgcac
     1381 atcggctggg tcgaagctgc gctcgaggcg cgtgcggacg gtcgtcggtg ccggcagggt
     1441 gcgcgagtag acgatcttct cggccgctcg ccagttccgc gcgaagtcgc gctccggctc
     1501 ggtctggccg gcgtcgatgc tcgcggtctc ccagtacgtc atcgtctcgt acatgcgccg
     1561 cccgtagagg tgcgtcccga tcggccgctc gaggtcgttg acgaaggcga gcacctcgtc
     1621 gtcgggcgcc gaccagtcga aggcgcctgc cgtgtcttcg acgtagccgt ccagcgaagc
     1681 gatcgccgcg tagatcaacc tggccatccg ttcacctgct tccgggatcg ctgatcgtcg
     1741 cgcgcttgcg ggtcggcgac gccggttcga acgggcgtcc cgtcgcgctc gcttctttgg
     1801 gcgaggtgct ggagttgccg tgcgtgcacg ccaggcagcc aatccttgcc gtacgtggcc
     1861 gatgggccga cccgcgggtc gccgggaact ccggcgtcac gccaggcgtg cacgtacgcg
     1921 cgatcctggt cgatccgtgc gcgtacctga tcagctccgc cgatggaccc gtggcccggg
     1981 acgaggacat cgacgtcgcc c
//
LOCUS       DATCBJ010000301         3113 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_79488, whole genome shotgun sequence.
ACCESSION   DATCBJ010000301 DATCBJ010000000
VERSION     DATCBJ010000301.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 3113)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3113)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3113
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_79488"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..722
                     /locus_tag="VII50_06065"
     CDS             <1..722
                     /locus_tag="VII50_06065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NAD-dependent dehydratase"
                     /protein_id="HEY5050446.1"
                     /translation="ASADSEWMGDLLEIERLAAQAPRTHPGLLVTVLRPAALVGGGVD
                     TIFTRHFEAPRLLAVKGSRTRWQFCHVDDLLSALELVATGGLSVPVHDDSADQPAART
                     PPSGHLVVAVGSAGWLEQEDVERVTGKRHIELPSSVAFSTAERLHRLGVTPAGSSDLQ
                     FLVHPWVVDPKRLADAGWRPAFDNESALAVAVAESAGRLAIGGHRLGRRDAAAGAAVG
                     ATVALVGTAALVRRARRRRHG"
     gene            715..1206
                     /locus_tag="VII50_06070"
     CDS             715..1206
                     /locus_tag="VII50_06070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein MoaE"
                     /protein_id="HEY5050447.1"
                     /translation="MAEDAVDAGRAPGTSPAGVVRLVGIRDLPLSVDEVLAAVADPAS
                     GGTTVFIGTVRSSDDGREVSSLRYEAHPDAIAVLQEVAEDVARTHHAIAVAAVHRTGL
                     LSIGDLAVVVAVAAGHRGEAFAAGRELIDEVKARVPIWKQQSFTDGAVAWVGIETTLD
                     VEG"
     gene            1252..1518
                     /locus_tag="VII50_06075"
     CDS             1252..1518
                     /locus_tag="VII50_06075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5050448.1"
                     /translation="MKVLLWLMIPVAALIVGTIWASIRSRPPRPAAMQDSMASFSRFR
                     RALAATTASSPPLRRGAPEDPADPSKLPANDSTVREPGTVGRPV"
     gene            1661..2191
                     /locus_tag="VII50_06080"
     CDS             1661..2191
                     /locus_tag="VII50_06080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5050449.1"
                     /translation="MEHVVFFPAPDGAPAFRRLATVDDAARFVEHLRNVENVSEASVH
                     TLTEVPLSFKTWYRVEIPNSEEGADNAQTDAAEVTAAPTTIAEVIESYATDDDSVSLD
                     QLELSGPSGVDGSSTGILVPEREFLPPVQIAFASPSVEPAFFGQPVAEPDRPAAQVLE
                     PAFGSRRERGTGYFVR"
     gene            2372..>3113
                     /locus_tag="VII50_06085"
     CDS             2372..>3113
                     /locus_tag="VII50_06085"
                     /inference="COORDINATES: protein motif:HMM:NF024578.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HEY5050450.1"
                     /translation="MRRQATTLLVSGVLAVILAFAGMHLPAPYVQDAPGPVSDTLATA
                     QGKPLITIAHPTATPKGRIYLVTVSESGGPRQNISAFQVLLGWWRRSDAVIPRRLLYP
                     DPGTTPQQVATQDNNDMIESQEEAKVAALRYLKYPLTPGVDIASVVLPALKGKLMNDD
                     IIIGVDNTTVTNSADLLKVDASHKPGDQIVLHIVRGTTQLDVPVTLQPPANGATTPTI
                     GISVVDSFTKPFAIDINLADVGGPSAGTA"
BASE COUNT          500 a         1032 c         1043 g          538 t
ORIGIN      
        1 aggcgagcgc cgatagcgaa tggatgggcg acttgctgga gatcgagcgg cttgccgcgc
       61 aggcgccccg gacgcatccc ggcctactcg tgacagtgct gcgtccggct gcgctcgtcg
      121 gcggtggcgt tgacaccatc ttcactcgcc atttcgaagc gccgagattg ctggcggtta
      181 aggggtcgcg cacccgctgg cagttttgtc acgtcgacga tttgctgtcg gcgctggaac
      241 tcgtcgcgac cgggggtctg agcgtgccgg tccacgacga cagcgcagat cagccggcgg
      301 cacgaacgcc gccgagcggc catctggtgg ttgcggtcgg cagtgccgga tggcttgaac
      361 aagaggacgt tgagcgggtg acggggaagc ggcacatcga gctgccgtcg tcggtggcgt
      421 tcagcacggc cgagcgtttg caccgtctcg gggtcacgcc ggctggctcc agcgatttgc
      481 agttcctcgt gcatccgtgg gtggtcgacc ctaagcggtt ggccgacgct gggtggcgtc
      541 cggccttcga caacgagagc gcgttggcgg tggcggttgc cgaatctgcg ggtcggctgg
      601 cgatcggcgg tcaccggctc ggacgccgcg atgcggccgc gggtgcggcg gtcggggcca
      661 ctgtggcgct ggttggtact gcggcgctgg tgcgccgcgc aagacggcgt cggcatggct
      721 gaagacgccg tcgatgccgg ccgcgcgccc ggcacgtcgc cagctggcgt cgtccggctg
      781 gtcggtattc gagaccttcc cttgtcggtc gatgaggttc tggctgcggt agcagatccc
      841 gcttcggggg gtaccacggt gttcatcggg accgtgcggt cctcagacga cggccgcgag
      901 gtgagctcgc tgcgctacga ggcgcatccc gacgcgatcg ccgtgctcca agaggtagcg
      961 gaggacgtcg cgagaacgca ccacgcaatt gccgtcgccg ccgtccatcg cactgggctg
     1021 ctttccatcg gcgatcttgc cgtcgtggtg gccgtcgccg ccggacaccg cggcgaggcc
     1081 ttcgctgcgg gcagggaact gatcgacgaa gtgaaggcgc gagtgccgat ttggaagcag
     1141 caaagcttca ccgacggcgc ggtcgcctgg gtgggcatcg agactacgct ggacgtcgaa
     1201 ggctgatcac acggaagggc cataagcaag gaagggacat ccgcgggatc catgaaagtc
     1261 ttgctgtggt tgatgattcc ggtggccgct ttgatcgtgg gaacgatctg ggcgtcgatc
     1321 cgtagccgac cgccgcgacc ggccgcgatg caggattcga tggcgtcgtt ctcccgattc
     1381 cgccgcgcgc tggcggccac caccgcaagc tcgccgccgt tgcgtcgcgg tgccccggag
     1441 gaccccgccg atccgagcaa gctccccgcc aacgacagca cggtgcgcga gccgggcacg
     1501 gtcggtcgtc cggtctgaag aattcgcccg tttgccgagg cacttgtggg ctcctcggct
     1561 caaggttgcc tgctgcggtt gccgatctat ggatcgtcag cgtccaatag ccagggtctc
     1621 tcgcgcacgt cgccgggccc agccgtctga gtgaggcaca gtggaacacg tcgtattttt
     1681 cccagcgccg gacggcgccc cagccttccg tcgtcttgcc accgtcgatg acgcagcccg
     1741 gttcgtcgag cacctgcgca acgtcgagaa cgtcagcgag gcgtccgttc acacgctgac
     1801 cgaggttcca ctgtcgttca agacgtggta ccgagtcgag atcccgaact ccgaggaagg
     1861 cgcggacaac gcgcagaccg acgctgccga agttacggcc gcacccacga cgattgccga
     1921 agtgatcgag agctacgcca ctgacgacga cagcgtgagc ctcgaccagt tggagctctc
     1981 cgggccttcg ggagtagatg gttcctcaac cggaatcctc gtccccgagc gcgagttcct
     2041 gccgcccgtc cagatcgcct tcgcctcgcc gtccgttgag cctgccttct tcggccagcc
     2101 ggttgccgag ccggaccgcc ctgcggccca ggttctggag cctgccttcg gtagccgccg
     2161 cgaacgtggc accgggtact tcgtgcggta aacccagccg catctccgac gcccctggcg
     2221 ccgcgaaccg caagctcccc gagcccccca agctccgaga gcaaccaagc tcccccaaga
     2281 gctgcgcccg agccgtggtc cctcccccag gaccgcggct ttggtgtttc ccggggcaac
     2341 attgttttag ccgcgcaact acgctggtct catgcgacgc caggcaacga ctctgctcgt
     2401 gtcgggcgta ttggccgtca tcctcgcgtt cgccggcatg cacctgccgg cgccgtacgt
     2461 gcaggatgcg ccgggcccgg tttctgacac gctcgctacg gcgcagggca agccgttgat
     2521 cacgatcgcg catccgaccg cgacgcccaa gggccgcatc tatctggtga cagtgagcga
     2581 gtccggcggt ccgcgtcaga acatctctgc tttccaggtg ctgcttggtt ggtggcggcg
     2641 cagtgatgcc gtgattcccc ggcgcttgct ctaccccgac ccgggcacga cgccgcagca
     2701 agtcgcgacg caggacaaca acgacatgat cgagtcgcag gaggaagcca aggtcgctgc
     2761 gctgcggtac ctgaagtatc cactcacgcc gggcgttgac atcgcgagcg tggttttgcc
     2821 tgcgctcaaa ggaaaactga tgaacgacga catcatcatc ggcgtcgaca acaccactgt
     2881 gacaaacagc gccgacttgt tgaaggtgga cgcttcccac aagcccggcg accagatcgt
     2941 cctgcacatc gtacgcggga ccacgcagct cgacgtgccg gtgaccctgc agccgcccgc
     3001 gaatggcgcc accaccccga cgatcgggat cagtgtggtc gacagtttca ccaagccctt
     3061 cgcgatcgac atcaacctgg ccgatgtggg cgggccaagt gcgggcaccg cct
//
LOCUS       DATCBJ010000302         2133 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_490371, whole genome shotgun sequence.
ACCESSION   DATCBJ010000302 DATCBJ010000000
VERSION     DATCBJ010000302.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2133)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2133)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2133
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_490371"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..639)
                     /locus_tag="VII50_06090"
     CDS             complement(<1..639)
                     /locus_tag="VII50_06090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEY5050451.1"
                     /translation="MTLDIEADVASLTAALVDIASVSGDEEPIADAVELALRQLDYLY
                     LERDGNVVLARTDTGRPQRIVLAGHLDTVPIADNVPSRRDGDILHGCGSSDMKSGVAV
                     MLRMAASLRPAASRSTPYDLTYVFYDCEEIESDRSGLGRLAKQRADVLRADLAIVLEP
                     TNGAVEAGCQGTVRVEITLSGTRAHSARSWLGHNAIHAAAPVLQRLADYRPRS"
     gene            complement(644..1480)
                     /locus_tag="VII50_06095"
     CDS             complement(644..1480)
                     /locus_tag="VII50_06095"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HEY5050452.1"
                     /translation="MGPLITSSLPTEIDAMWDRRADLNPADAAARALVIDAVDQLDQG
                     LARVAWIEPSTDLVVVDERAKRAILLSFKVLAMQESSAGAFRYHDRMPLKTQFDGVRV
                     VPGAIVRWGAFIAPGAVLLPSFVNIGGYVDEGSMVDTWATVGSCAQVGKRVHLSGGVG
                     LGGVLEPPNAVPVVVEDDAFIGSRSMVVDGARVRRGAKLGAGCILTASTHVFDAQSGD
                     ELPRGEAPAWSVCVGSTRVKSFPGGDFGMPCLLVLRRLDEGQAHDKLVLNDVLREHGV
                     AE"
     gene            1678..>2133
                     /locus_tag="VII50_06100"
     CDS             1678..>2133
                     /locus_tag="VII50_06100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5050453.1"
                     /translation="MLVTTAVVFGVKSLTPHRTVADSCQVVGETTGTVYALKPDQLLN
                     ASIIANVAMRRGLPEQAVIVALATAQQESKLTNIDYGDADSVGLFQQRQSQGWGSQAQ
                     ILDPVYASGKFFDALVKVRNWEGLTVAAAAQAVQRSAFPDAYASWQPLGT"
BASE COUNT          369 a          761 c          681 g          322 t
ORIGIN      
        1 cgagcgcggc cggtagtccg cgagccgttg cagcaccggc gccgccgcgt gaatcgcgtt
       61 gtgcccaagc catgacctgg ccgagtgcgc acgggttccg ctcagcgtga tctcgacccg
      121 caccgtgccc tggcacccgg cttcgaccgc gccattggtc ggttcgagca cgatcgcgag
      181 gtcggcgcga aggacgtcgg cgcgttgctt cgcaagacgg ccaaggccgc tgcgatcgga
      241 ctcgatctcc tcgcagtcat agaagacgta ggtcaggtcg tacggcgtgc tacggctggc
      301 cgccggtcgc agcgacgcgg ccatccgcaa catcaccgcg acaccggact tcatgtcgct
      361 cgatccgcaa ccgtgcagga tgtcgccgtc gcgtcgcgac ggcacgttgt cggcgatcgg
      421 caccgtgtcg aggtgtcctg ccagcacaat gcgttgcggc cgcccggtgt cggttcgggc
      481 gagaacgacg ttgccgtccc gctccagata gaggtagtcg agttgacgta acgccaactc
      541 cacggcgtcc gcgatcggct cttcatcgcc actgaccgac gcaatgtcga cgagcgccgc
      601 cgttagcgac gcgacgtccg cctcgatgtc caatgtcacg gcgctactcc gctacgccgt
      661 gctcgcgcag cacatcattg agcaccagtt tgtcgtgcgc ctggccctca tcgaggcgcc
      721 gaagaacgag caggcacggc atgccgaaat cgccgccagg aaaggacttc accctcgtcg
      781 atcccacaca caccgaccac gcgggggcct cgccgcgggg caactcgtcg ccactttgcg
      841 catcgaacac atgggtcgac gcggtcagga tgcagccggc gccgagcttg gcgccgcgcc
      901 gcacccgcgc accgtcgacc accatcgaac gcgaaccgat gaacgcatcg tcttcgacca
      961 caacggggac cgcgttcggc ggctccagca cgccgccgag ccccacccca ccggaaaggt
     1021 gcacgcgctt gccgacctgc gcgcacgagc cgacggtcgc ccacgtgtcg accatgctgc
     1081 cttcgtcgac gtacccgccg atgttgacga agctcggcaa cagcaccgca cccggcgcga
     1141 tgaaggcacc ccatcgcacg atcgccccag gcactacccg caccccgtca aactgcgtct
     1201 tcagcggcat ccggtcgtga tagcggaacg cgccggccga cgactcctgc atcgccagca
     1261 ccttgaacga cagcaaaatg gcacgcttcg cccgctcgtc gaccacgacg agatccgtcg
     1321 acggctcgat ccaggccacc cgggccaggc cttggtcgag ctggtcgacc gcatcgatca
     1381 ccagagcgcg ggcagcggca tcggccggat tgaggtcggc gcgacggtcc cacatcgcgt
     1441 cgatctctgt gggaagcgag ctggtgatga gcgggcccat ggccgctgac actacctagt
     1501 gatcgcgctt gcccgagtag caccgcgccc ggcgatagat tcgcggtagc catgacgacg
     1561 tccgatcggc ctatgcggcc cgtgcgacgc gcgcgacgtg ctcggcgcgc gcgacggcct
     1621 cgacagcaga gtcgccgcgt ccggctgacc aggaccatcg tcgttttcgt ctcgctcgtg
     1681 ctggtcacca cggcggtcgt gttcggcgtg aagtcgctga caccgcaccg cactgtcgcc
     1741 gacagttgcc aggtcgtggg agagaccacg ggcacggtct acgcactgaa gccggaccaa
     1801 ctcttgaatg cctcgatcat cgcgaacgtc gcgatgcgcc gcggcctgcc cgagcaggcc
     1861 gtaatcgtcg ccctggcaac cgcacagcag gagtcgaagc tgaccaacat tgactacggg
     1921 gacgccgatt cggtcggctt gttccagcag cggcagagcc aaggctgggg gtcgcaggcg
     1981 cagatcctcg accccgtgta cgcgagcggc aagttcttcg acgcgctggt caaggtccgc
     2041 aactgggagg gcctcacggt ggctgcggcg gcgcaggcag tgcagcgcag cgcattcccc
     2101 gatgcgtacg cgtcgtggca accgctgggc acc
//
LOCUS       DATCBJ010000303         2788 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_487972, whole genome shotgun sequence.
ACCESSION   DATCBJ010000303 DATCBJ010000000
VERSION     DATCBJ010000303.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2788)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2788)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2788
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_487972"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..274)
                     /locus_tag="VII50_06105"
     CDS             complement(<1..274)
                     /locus_tag="VII50_06105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5050454.1"
                     /translation="MSEHPASAVDDARRNAVRAYVEAHKDEFVAQLSEWIRIPSVWTD
                     PERRDDVQRSAQWFADAARAVGFPTVEIWQASDGAPAVFAEWLSDNP"
     gene            complement(284..>2788)
                     /locus_tag="VII50_06110"
     CDS             complement(284..>2788)
                     /locus_tag="VII50_06110"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF024117.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HEY5050455.1"
                     /translation="VLLDEYQDTSHAQLVFLRALFGAFHRGLGVAPLLTAVGDPSQAI
                     YGWRGASQGTLVAFPQQFPDADKPASVLTMSTSFRNERSVLAVANAIAEPLRDGRVST
                     PELRARTAAVDGIVRCGLYPTVDDEAQAIGDRVFGLWLADQENRAAGTPTRSIAVLVR
                     AWRQLPRIEAALRARGVPIEIVGVGGLLLEPEVVDVVSTLRVMVDPARGDALMRLLTG
                     ARWRIGGRDLAALGGWARHLARTPARAGLPADGVAHGVAVDVTTVEPDPDEVDDRSII
                     DALDALPAPGRFTPKGLSAIGHERLARLAGELRALRSRLGQPLPDLVADVVATIGLDV
                     EVLSRGGDLSASRANLDRLIDVAAEFAAGGDDVSVAAFLDYLDAAAVEERGLDRPDEP
                     ESTVPEVRAGVDVRRDRVQLLTVHASKGLEWDVVCVPGLVENLFPGVRPGDVRGWLTQ
                     RGSLPWPLRGDRDGLPVADLDGHVDQLQAVAAIERFRDAATAHLLREERRLAYVAVTR
                     ARSTLICSGYRWDDTKKPREVSAFLAAVHDVCESGRGEVDCWTELPPDGTPNPMRELG
                     PVSATWPLDPLAVRRPAVEAAAALVELTAAEAATLDGAAAMATAIDEWSRDVEVLLSE
                     RAARTQRARLDVELPRQLSVSQLVTLRRDPDELARQLRRPLPAAPAPLARRGTAFHLW
                     LEQRFSSPRLLDLDEVPGSADADAAPDADLHELQKSFLATSWADRDPVEVEVPFELVI
                     DDIVVRGRMDAVFRDSDGGFTVVDWKTGRRPAGADARAAAIQLAAYRLAWADLTGTDL
                     AAVRAVFCYLRDGETVAPSDLLDRDGLVELLRSVPA"
BASE COUNT          475 a         1001 c          920 g          392 t
ORIGIN      
        1 tcgggttgtc gctgagccat tccgcgaaaa cggccggcgc gccatcgctc gcctgccaga
       61 tctcgacagt cggaaacccg acagctcgtg ccgcatcggc aaaccactgt gccgagcgct
      121 ggacgtcgtc tcgtcgctcc gggtcggtcc acaccgacgg gatgcgaatc cattccgaca
      181 gctgcgcgac gaactcgtct ttgtgtgcct cgacataggc ccggacggcg ttgcgccgcg
      241 catcatcaac cgccgaagcg gggtgctcac tcatacctgc accctaagcg gggaccgatc
      301 gcagcagttc gaccaggccg tcgcggtcga gcaggtcact cggcgcgacc gtttcaccat
      361 cacgcaggta gcagaacacc gcgcgcacgg cggcaaggtc ggtacccgtg agatcggccc
      421 atgccaaccg gtaggcggcc agctggatcg cggcggcgcg cgcatcagca ccggccggac
      481 ggcggccggt cttccagtcg accacggtga acccgccgtc gctgtcgcga aacaccgcgt
      541 ccatgcgacc gcgcacgacg atgtcgtcga tgacgagctc gaagggcacc tcgacctcga
      601 cggggtcgcg atcggcccat gacgtggcga gaaacgactt ctggagctcg tgcaggtcgg
      661 cgtcaggtgc ggcatcggcg tcggccgagc cgggcacctc gtcgaggtcg agcaagcgcg
      721 gcgaggagaa ccgttgctcg agccacaggt ggaacgcggt gccgcggcga gcgagcggtg
      781 ccggcgccgc gggcaacggt cggcggagct ggcgcgcgag ctcgtcagga tctcgacgca
      841 acgtgacgag ctgactgacc gacagctgcc gcggcaactc gacgtcgagg cgggcccgct
      901 gggtgcgtgc cgcgcgctcg gaaagcagca cctcgacgtc gcgactccac tcgtcgatcg
      961 ccgtcgccat tgccgctgct ccatcgagtg tcgccgcctc cgcggccgtc agctccacca
     1021 gcgcggccgc ggcctcgacg gccggacgcc gaactgccaa cgggtcgaga ggccaggtcg
     1081 cactgaccgg cccaagctcg cgcatcgggt tcggtgtgcc gtcggggggc aactcggtcc
     1141 agcagtcgac ctcgccgcgg cccgactcgc agacatcgtg cactgcggca agaaatgcac
     1201 tcacctcgcg tggcttcttc gtgtcgtccc atcgataacc gctgcagatc agcgtgctgc
     1261 gcgctcgggt gaccgcgacg taagcaagcc gtcgttcctc gcgcaagaga tgcgccgtcg
     1321 ccgcgtcgcg aaatcgctcg atcgcggcaa cggcctggag ctggtcgacg tgaccatcga
     1381 ggtctgccac gggcagcccg tcacgatcgc cgcgcagtgg ccacggcaac gacccgcgct
     1441 gagtgagcca acctctgacg tcgccgggcc gcacaccagg aaacagattc tccacaagac
     1501 cgggcacgca caccacgtcc cactcaagtc ccttcgacgc atgaacggtg agcagctgca
     1561 cgcgatcgcg ccgtacgtcg acccccgcgc gcacttccgg caccgtcgac tcgggttcgt
     1621 cgggccggtc gaggccgcgt tcttcgaccg cggcggcgtc gaggtaatcg aggaacgcgg
     1681 ccacgctcac gtcatcgccg cccgctgcga actcggcagc gacatcgatc aatcgatcaa
     1741 ggttggcccg cgacgcgctc agatcgccgc cgcgggagag cacttcgacg tcgagaccga
     1801 tcgtcgcgac cacatctgcg accagatcgg gcagcggctg accgagccta gaccgcaacg
     1861 cgcgcaactc gccggccaac cttgccagcc gctcatgacc gatcgccgaa agccccttcg
     1921 gcgtgaacct gccgggcgcg ggcagggcgt cgagcgcgtc gatgatgctg cggtcgtcga
     1981 cctcgtcggg atccggttcg accgtcgtca cgtcgaccgc gaccccgtgt gcgacaccgt
     2041 ccgcaggaag gccagcccgc gccggcgtcc gcgccaagtg acgggcccag ccgccgagag
     2101 ccgccaggtc tcggccgccg atgcgccagc gtgccccggt cagcaaccgc atgagcgcgt
     2161 cgccgcgcgc ggggtcgacc atcacccgca gcgtggacac gacgtcgacg acttccggct
     2221 cgagtaacag tccaccgact ccgacgatct cgatcggcac cccacgtgcg cgcaacgcgg
     2281 cctcgatgcg tggcaactgt cgccacgctc tcacgaggac cgcgatgctc ctggtaggtg
     2341 tacccgcggc ccgattctcc tggtcggcaa gccacagacc gaagaccctg tcgccgatcg
     2401 cctgtgcctc gtcgtcgacc gtcgggtaga ggccgcatcg cacgatgccg tcgaccgcgg
     2461 cggtgcgtgc gcgaagttct ggcgtcgaga cgcggccatc gcgcagcggc tccgcaatcg
     2521 cgttcgcaac ggcaagaacg ctgcgctcat tgcgaaaact ggtgctcatt gtcagcacgg
     2581 acgccggctt gtcggcgtcg gggaattgct gcgggaacgc caccagcgtg ccctggctcg
     2641 cgccacgcca gccgtagatc gcctgcgacg gatcaccgac cgcggtgaga agcggggcga
     2701 cgccgagccc gcggtgaaag gcaccgaaca atgcacgcaa gaacaccagt tgggcgtggc
     2761 tggtgtcctg gtattcatcg agcagcac
//
LOCUS       DATCBJ010000304         2379 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_336079, whole genome shotgun sequence.
ACCESSION   DATCBJ010000304 DATCBJ010000000
VERSION     DATCBJ010000304.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2379)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2379)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2379
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_336079"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1276)
                     /locus_tag="VII50_06115"
     CDS             complement(<1..1276)
                     /locus_tag="VII50_06115"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HEY5050456.1"
                     /translation="MTTTHEPDIKPRSRDVTDGLEKAGARGMLRAVGMGDDDWVKPQI
                     GVASSWNEITPCNLSLDRLAKSAKEGVHAAGGYPLEFGTISVSDGISMGHEGMHFSLV
                     SREVIADSVETVMMAERLDGSVLLAGCDKSLPGMLMAAARLDLAAVFLYAGTTLPGRI
                     GDRDVNIVDAFEAIGGCLAGRVTRAEVDAIERVFCQVEGACAGMFTANTMASAAEALG
                     MSMPGSAAPPAPDRRRDGFARASGEAVVGLLRKGITARQILTKPAFENAIAVVMALGG
                     STNAVLHLLAIAREAEVDLDLDDFNRIGDHVPHLADVKPFGRYVMSDVDHVGGIPVVM
                     KALLDAGLIDGSALTVTGHTVAENLADLAPSDPDGKVIHAMADPIHRTGGLTILRGSL
                     APDGAVVKTAGLDRATFEGTARVFDGEAAAMAA"
     gene            complement(1315..>2379)
                     /locus_tag="VII50_06120"
     CDS             complement(1315..>2379)
                     /locus_tag="VII50_06120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5050457.1"
                     /translation="LSHLISYFTTVHQDATGQTQVLLATDTPTLRIAYVTSNSRNGVI
                     QSWFYGPVGSDNLVEGATSYGGSLLTDSVLIDGLIDSGGHEQFVVIAPPATTGMQIAD
                     FDFNDPAVAPGFAPLPYQNGIAVKDMPPGTSALSFVVDVTVGTATFTLKDAPDIQLRP
                     QFTGAVTSDGPPSAPLPTPAVERGHPDPTLMAEALRDAAAWEGSTPVVAARPVVLWAG
                     ADAAGTKVVVLRVKTKAFDLMVVEWSNAAPGDHAEYLVDPSAPDAPVAFAYRAVDGTR
                     LGVIGSAGAVTVALVANGRDSKPVAFDSTGFASFRLPTPPADPNNDGAGALGVVAQVR
                     LLDASGHPLAALSVPPTLGG"
BASE COUNT          409 a          799 c          791 g          380 t
ORIGIN      
        1 cggccgccat ggccgcggct tcgccgtcga acacacgagc cgtgccttcg aacgtcgctc
       61 ggtcgagccc cgcggtcttc acgacggcgc cgtcgggcgc caacgagccg cgcaggatcg
      121 tcaagcctcc ggtgcgatga atggggtcgg ccatcgcgtg gatcaccttg ccatcagggt
      181 cggacggcgc aaggtcggcg agattttcgg ccaccgtgtg acccgtgact gtgagagccg
      241 acccgtcgat caacccggca tcgagcagcg ccttcatgac gaccgggatc ccgccgacat
      301 ggtcgacgtc gctcatgacg tagcgaccga aaggcttcac gtcggccagg tgcggcacgt
      361 ggtcgccgat gcggttgaag tcatcgaggt cgagatcaac ctcggcttcg cgagcgatcg
      421 cgagcaggtg cagcaccgcg ttcgtcgacc cgcctaacgc catgacaacc gcgatcgcgt
      481 tctcgaatgc gggcttggta agaatctgcc gggccgtaat tcccttgcgc aacaggccga
      541 cgacggcttc gccggatgcg cgagcgaacc cgtcgcgtcg gcgatcaggt gccggtgggg
      601 cggccgaacc aggcatcgac atcccgagcg cctcggcagc actggccatg gtgttcgcgg
      661 tgaacatgcc cgcgcaggca ccctcgactt ggcaaaagac gcgctcgatc gcgtcgacct
      721 cggctcgggt gacgcggccg gccaggcacc caccgatcgc ctcgaaggca tcgacgatgt
      781 tcacgtcgcg gtcgccgatc cggccgggca atgtggtacc ggcatacaag aacacggccg
      841 cgaggtcgag acgggcggcg gccatcagca tgccgggcaa cgacttgtcg cagccggcaa
      901 gcaggaccga tccgtcaagc cgctcggcca tcatgacggt ttcaacggag tcggcgatga
      961 cctcgcgcga caccagcgag aagtgcatgc cctcgtggcc catggagatg ccgtcggaca
     1021 ccgagatcgt gccgaactcg agtgggtacc cgcccgcggc gtgcacgccc tccttcgccg
     1081 actttgcgag cctgtcgagt gacaggttgc agggcgtgat ctcattccac gagctcgcga
     1141 ccccgatctg cggcttgacc cagtcgtcgt cgcccatgcc gacggcgcgc aacatcccgc
     1201 gtgcgccggc cttttcgagg ccgtcggtca cgtcgcggct acgtggcttg atatctggct
     1261 cgtgagtcgt cgtcatgacg cgtcacccta cgtcaacttc gtgggccaca gtggtcagcc
     1321 gccgagcgtc ggcggtaccg acagcgcagc gagcgggtga ccggacgcat cgagcagtcg
     1381 tacctgggca acgaccccga gcgcaccggc cccgtcgttg tttggatccg ccggcggagt
     1441 cgggaggcgg aagctggcga atcccgtcga atcaaacgcg actggtttcg agtcgcgccc
     1501 gttggcgact agcgccaccg ttacggcgcc ggccgatccg atgacgccga gccgcgtccc
     1561 gtcgaccgca cgataggcaa aggcaaccgg ggcatctggc gcgctcgggt cgacgaggta
     1621 ctcggcatga tcgccgggtg cggcgttgct ccattcgacg accatcaagt cgaacgcctt
     1681 ggtcttcacc cgcagcacca cgaccttcgt ccccgccgcg tcggcgccag cccacagcac
     1741 caccgggcgt gcggcaacca ccggggtcga cccttcccag gccgccgcgt cgcgcagtgc
     1801 ctcggccatc aacgtggggt cagggtgacc gcgttcgacg gccggcgtgg gcagcggcgc
     1861 cgatggcggt ccatcgctcg tcaccgcgcc ggtgaactgc ggcctgagtt ggatgtcggg
     1921 cgcgtctttg agcgtgaacg tcgcagtgcc gacagtgaca tcgacgacga agctcaacgc
     1981 tgaggtgccc ggcggcatgt ccttcaccgc gatcccgttc tggtagggca gcggcgcgaa
     2041 ccctggcgcg acggcggggt cgttgaagtc gaagtccgcg atctgcatgc ctgtggtggc
     2101 gggcggcgcg atgacgacga actgctcgtg cccgcccgaa tcgatcaagc catcaatgag
     2161 cacgctgtcc gtcagcaggc tgcccccgta actcgtcgcg ccctcgacca agttgtcaga
     2221 accaaccggc ccgtagaacc acgattggat gacaccgttg cgcgaattcg atgtgacgta
     2281 agcaatacgc agcgtcggtg tatcagtagc aagcagcacc tgcgtctgac cagtggcgtc
     2341 ctggtgcacg gtcgtgaagt aggagatcag gtgcgagag
//
LOCUS       DATCBJ010000305         2226 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_359654, whole genome shotgun sequence.
ACCESSION   DATCBJ010000305 DATCBJ010000000
VERSION     DATCBJ010000305.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2226)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2226)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2226
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_359654"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..214)
                     /locus_tag="VII50_06125"
     CDS             complement(<1..214)
                     /locus_tag="VII50_06125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5050458.1"
                     /translation="MRSKVQTITPAKAAEWLEANTTNRPVSRTVVRSFAEAMKRGEWI
                     VTHQGIAFDVSGVLIDGQHRLAAVVEA"
     gene            complement(332..1102)
                     /locus_tag="VII50_06130"
     CDS             complement(332..1102)
                     /locus_tag="VII50_06130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5050459.1"
                     /translation="MTTFPASGGKPPLLGQVALPPLGGGLVVVWQLLFDIAAAVPIGR
                     WQLVGGQMVLLHGLCAGRQPLRATHDIDVLADLLTASDGLRVVVAAIEGLGFEPVEDS
                     SGHIYRFARSRDNAAVDVLAPDHTPPTWRLVTARGKETIRVDGGRQAMQRPALIGVTM
                     ADATAEIPVPDLLGALVLKAAAWMVDSRDRERHSGDAAFLVSLIGDPRQERSRFTGSD
                     RKRLNKLSSVLGDPAAPEWAALGDRGPDAFTSWTLLLS"
     gene            complement(1099..1917)
                     /locus_tag="VII50_06135"
     CDS             complement(1099..1917)
                     /locus_tag="VII50_06135"
                     /inference="COORDINATES: protein motif:HMM:NF024139.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEY5050460.1"
                     /translation="MSELAGQARETAETTGWTYAACWGLAKYPRRRSISAYAVEGVSG
                     VSVTTSEAARRLGVSANEVRRLLAAGDLKAVSVGRIFLVDEASVRTRLRAPKGRGRAL
                     APQIAWAALLEASGERASWLDRSTRSRLRRWLRGHDPDAIAAGCRRRAVRVDLRVLPT
                     YRDAVMSTQGVVVGGMSARDQIDSGIVAVGDVADEMYCSKETLDKLSRDYGLSQLGAA
                     NLIVRMPTFDDDQVLTRRVMPAAVVAVDLLESDDVRTRRLGAQILTRAVADQDR"
BASE COUNT          390 a          791 c          693 g          352 t
ORIGIN      
        1 ccgcctcgac gacggccgcg agccggtgct gcccgtcgat gagcaccccg gacacgtcaa
       61 acgcgatccc ctggtgggtg acgatccact cgccgcgttt catcgcctca gcgaacgacc
      121 gcaccaccgt tcgggacacc ggccggttgg tggtgttggc ctccagccac tcagctgcct
      181 tcgccggggt gatcgtctgc accttgctgc gcaccgaact cgctccttcg ccgccccatg
      241 tttgccagcc gcaagactag ctgtcgcacg ccaccacgcc gcatccgtcg caccgcacga
      301 cgcgagcacc atgcggaccg cgtcgcggtg cctaggagag aagaagtgtc cagctggtga
      361 atgcgtcggg cccgcgatcg ccgagggcgg cccattccgg cgccgcggga tcacccaaga
      421 cggagctcag tttgttgagc cgcttgcggt cgctgcccgt gaaccggctg cgctcctgcc
      481 gtgggtcgcc gatgagactg accaggaaag cggcatcgcc gctgtgccgt tcccggtcgc
      541 ggctatcgac catccatgcc gcggctttga gcacaagcgc gccgagcagg tccggtaccg
      601 ggatttccgc cgtagcgtct gccatcgtca ccccaataag tgctgggcgt tgcatcgctt
      661 gacggccgcc gtcgacgcgg atggtctcct tacctcgtgc ggtgacgagt cgccacgtgg
      721 gcggcgtgtg gtcaggagcc aagacgtcca cggcggcgtt gtcgcgactg cgcgcgaacc
      781 ggtagatgtg gcccgaggag tcctcgaccg gctcgaaacc caagccttcg atggccgcta
      841 ccaccacacg caggccgtcg gatgcggtga gcaggtcagc gaggacgtcg atgtcgtggg
      901 tcgctcgaag cggctgccga ccggcgcata gaccatgcag caacaccatc tgcccgccga
      961 ctagttgcca gcgcccgatc ggcacggcgg cggcgatgtc gaacagcaac tgccatacca
     1021 cgacaagacc acccccaaga ggcgggagcg cgacttgtcc aagcagcggc ggcttcccgc
     1081 cgcttgcggg aaaggtggtc atctgtcctg gtcggcgacg gcgcgggtga gtatctgcgc
     1141 gccgaggcgc cgcgtgcgga cgtcatcgga ttccagcaga tcgacggcga ccaccgcagc
     1201 cggcatcacc cgtcgagtca acacttggtc gtcatcgaag gtcggcatcc ggacgatcag
     1261 gttggcggcc ccgagctggc tcaatccgta gtcgcggctc agcttgtcca aggtctcctt
     1321 cgagcagtac atctcgtcag cgacatcacc cacagcgacg atgcccgagt cgatctggtc
     1381 ccgcgcggac atgccaccga ccactactcc ctgggtcgac atgacggcgt cgcggtaggt
     1441 cgggagtacc cgcagatcta cgcggacagc tcgtctgcga caccccgcag cgatcgcgtc
     1501 ggggtcgtgg ccgcgcagcc atctacgcaa cctcgacctt gtcgaccggt ccagccatga
     1561 cgctcgctca ccggacgcct ccaagagcgc agcccatgcg atttggggtg cgagcgcgcg
     1621 accgcgacct ttgggcgccc gcaaacgagt acgcacgctg gcttcgtcaa caagaaagat
     1681 ccggcccacc gagactgcct tgaggtcgcc ggccgcgagc aaccgccgca cctcgttggc
     1741 actgacaccc agccgtcgag ccgcctcaga cgtcgtcacc gacacacccg acaccccctc
     1801 caccgcgtag gcagaaatac tacgccgacg tgggtatttt gccaaccccc agcaggccgc
     1861 gtatgtccag ccggtcgtct cggcggtctc ccgagcctga cccgctagct cggacacaag
     1921 ctcagccgcg ccatccggtc cgcgaccggt tggcgcgcgc aaggccgatg agaacccggg
     1981 agtcgcgaaa cccggcgggc ggggccgtag cggggccgta cggccggtaa agtgcggcct
     2041 agtacggacc acagcggtca agatcaaaaa cagatacggg caggtcagaa gccattttca
     2101 cgctcaaacg cgaacctgtc ccgctagcct tccaagctgg ccatgccggt tcgatcccgg
     2161 tcacccgctc caccgagatt ctcctcgtag agcagggttt tctctgtgca catgccttcc
     2221 gccgtc
//
LOCUS       DATCBJ010000306         3486 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_444305, whole genome shotgun sequence.
ACCESSION   DATCBJ010000306 DATCBJ010000000
VERSION     DATCBJ010000306.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 3486)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3486)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3486
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_444305"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..433
                     /locus_tag="VII50_06140"
     CDS             <1..433
                     /locus_tag="VII50_06140"
                     /inference="COORDINATES: protein motif:HMM:NF039330.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF6036 family nucleotidyltransferase"
                     /protein_id="HEY5050461.1"
                     /translation="IGGTAMALAYDARRATRDIDAVLVPHNVVLEEAHALAAELGLPQ
                     WWLDEQASIYVAPGGDAEAPLSFDHPGLRVSTASPEHLLAMKTIAARQRDIEDLRILI
                     GMLGLTTAEQVIAVCSRVFPDELLPDRARLALEDLFDANAR"
     gene            complement(672..2561)
                     /locus_tag="VII50_06145"
     CDS             complement(672..2561)
                     /locus_tag="VII50_06145"
                     /inference="COORDINATES: protein motif:HMM:NF016047.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding repeat-containing protein"
                     /protein_id="HEY5050462.1"
                     /translation="MIRPGRAMWFATVAALAVAAVALPNAASADSTLPVYNVTQPSSV
                     PKASSVAVDPVSHRVYVASQTNRSVAVVDGLTGSTLATIVVPYTPMSVGIDSASHRLF
                     ILGDVSVLKPLKFTISLSIVDTTTNAIITTVPGPAWPGPLAVDTVNHRVFAPPYEFDE
                     TGKIVTMSAAAGGFSAAIDPSGHRLYLTTSSGVVAVWDTTTHLLVTTVTVSGSPTALA
                     VDPGTHSVYVSDLNHALVSVINGTTDKVTATIPVGGAPTALAVDSSAGLVYVSNVPNV
                     DVVSGPVPLGTVTVIDATHNTVLPSVTVSGAGDNGLDIGIAVDSVTHAVWLAAGQLSE
                     LSPVVSRRAGTDRFGTATAISGAAYDTLGADAVVLARSDTYPDALVGAPFAAAKNAPL
                     LYTTGASLPAETSAEITRVLAPGKTVYLLGGANAIPASIATQLTGMGYAVSRISGSDR
                     YATAVAVADAMGDPSTVFLATAANYADALAAGPAAAHVTGAILLTSGTTLPPATSAYL
                     AAHPGTTYAVGEPAAAADPSASAISGTDRYATAALVAHQFFAMPTTVGLASGVTFADA
                     LPAAAFLARVGGPLLLTDPNALPSTASAYLAAAQSGVTAALVFGGASAVSTAAQTEFS
                     AALGR"
     gene            complement(2583..>3486)
                     /locus_tag="VII50_06150"
     CDS             complement(2583..>3486)
                     /locus_tag="VII50_06150"
                     /inference="COORDINATES: protein motif:HMM:NF040138.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HEY5050463.1"
                     /translation="NVLPTAAFTSTSTNPSATFNAGGSTDTDGTITGYAWTFGDSTTG
                     TGVSPSHTYAIAGTYTVGLTVTDNSGGTNTVTHPITVSTAVTPAQYASDGFGRSVANG
                     FGTADLGGPWSLTGKSSAFSVAASAGMITVAAAGLSDAAYLANVSSSDTQVQVGVSLN
                     QAQNGGGTYVSVIGRRISATTDYEVKLRFLAGGSVTESLVAVVGGTSTTLKTITVAGL
                     TYTPGAVLEVKLQVTGTSPTTLNSRVWLQGSTEPTTWQVSTTDGTAGLQLAGAVALLV
                     YLSASSTTVPETASFTAFAAGATH"
BASE COUNT          633 a         1117 c         1161 g          575 t
ORIGIN      
        1 catcggcggg accgcgatgg ctctcgccta cgatgcccgc cgcgcgaccc gcgacatcga
       61 cgccgtactc gtgccgcaca atgtcgtgct tgaagaggct cacgcattgg ctgccgaact
      121 cgggcttcca cagtggtggc tcgacgagca ggccagcatc tatgttgcgc cgggcggtga
      181 cgcagaggct ccgttgagct tcgatcaccc aggactgcgg gtaagcactg catcacccga
      241 acatctgctg gccatgaaga caatagctgc gcgccaacgc gacatcgaag acctacgaat
      301 actcatcggc atgctcgggc ttacgacggc cgagcaggtg attgcggtgt gcagccgagt
      361 cttccccgac gagttgttgc ccgatcgggc gcggctggcc ctggaggatc tcttcgacgc
      421 aaacgcccgt tagggaacta tcagcgcgct caatgccgcc ggccgcaggc tggctcgacg
      481 atcggcggcg gatacccaac gctgagccgg gcggtctgct gattccccgc agtttcgcaa
      541 gctatgcaca ggacggtgcg cctgcagccc catgcgtcga cctaatagca tcgcgccagt
      601 ggggacatat gcaccgcgcg cctcgggcgt gtcgggtgtg tttgtcccca ctcgatcgaa
      661 ccgacgggct gttaccgacc gagtgccgcg ctgaactcgg tttgtgctgc ggtcgacacg
      721 gcgctcgcgc cgccgaacac caacgccgcc gtcacgcccg actgcgcagc agccaggtag
      781 gccgatgccg ttgacggcaa cgcattggga tcggtgagca gcaatggtcc accgacgcga
      841 gccaagaagg cggccgccgg caaggcgtcg gcgaacgtga ccccgcttgc caacccgacc
      901 gtcgtcggca tggcgaagaa ctggtgcgcg acgagagctg cggtggcgta acggtcagtg
      961 ccagaaatcg cggacgccga ggggtcggcc gccgctgccg gttcgccgac tgcgtacgtg
     1021 gtgccgggat gcgcagccag ataggcgctc gtcgctggcg gcaaagttgt gccggacgtg
     1081 agcaagatcg cacccgtcac atgagccgca gcagggccag cggcgagtgc gtcggcgtag
     1141 ttcgcggccg tcgcgaggaa caccgtgctc gggtcgccca tggcgtcggc gaccgcaacc
     1201 gcagtggcgt aacggtcgct cccgctgatg cgactcacgg cataacccat gccggtcaac
     1261 tgcgtggcga tgctggccgg gatcgcattc gcgccgccga gcaagtacac cgtcttgcct
     1321 ggcgccagaa cgcgggtgat ttctgcgctg gtctctgcgg gaagtgatgc acctgtcgtg
     1381 tagagcaacg gcgcattctt ggccgccgcg aatggagcac caaccagcgc atcggggtag
     1441 gtgtccgacc gcgccagcac cacggcgtcc gcgcctagtg tgtcgtaagc agcacccgag
     1501 atcgcggtcg cggttccgaa ccggtcggtg cctgcacggc gggagacgac gggcgaaagt
     1561 tcgctcagct gaccggcggc aagccagaca gcgtgagtaa ccgagtcgac ggctatgccg
     1621 atgtcgaggc cgttatctcc tgccccagag acggttaccg aagggagcac ggtgttgtgc
     1681 gtcgcgtcga tgacggtgac cgtgccgagt ggcaccgggc cggacaccac atcgacgttc
     1741 ggaacgttgc tcacgtacac caggccagcg ctcgaatcga cagcgagcgc tgtgggagca
     1801 ccaccgaccg ggatcgtcgc ggtgaccttg tccgtcgttc cattgatgac ggaaaccagg
     1861 gcatggttca ggtccgacac gtacaccgag tgggttcccg gatcgaccgc gagcgcggtg
     1921 ggcgatccgc tgaccgtcac ggtcgtgacc agcaggtgcg tcgtggtgtc ccagacagcg
     1981 accacgcccg aactcgtcgt gagataaagg cggtgaccgg acgggtcaat cgctgcactg
     2041 aacccgcccg cggccgccga catggtcacg atcttgcccg tctcgtcgaa ctcgtaaggt
     2101 ggcgcgaaga cgcggtggtt gaccgtatcg actgcgagtg gccccggcca ggccggccca
     2161 ggaaccgtcg tgatgatggc gttcgtcgtg gtgtcaacga tcgacaacga gatggtgaac
     2221 ttcaagggct tcagcaccga cacgtcaccc agaatgaaga gccggtgcga ggcggagtcg
     2281 atgcccactg acatcggcgt gtatggcacg acgattgtgg caagcgttga tccggtgagt
     2341 ccgtcgacta ccgcaacgga gcggttcgtt tgacttgcga cgtagacccg atgggacacc
     2401 gggtcgaccg caaccgagga cgctttcggg acgctgctgg gctgcgtgac gttgtaaacc
     2461 ggcagcgtcg aatcggccga ggcggcgttc ggtagcgcaa cggcggcgac ggctaacgcc
     2521 gccaccgtcg cgaaccacat cgcgcgccca ggccggatca cgggtctcac cctgtcagat
     2581 tcttaatgcg tggcaccagc cgcgaacgcg gtgaacgaag ccgtctcagg caccgtcgtc
     2641 gagctcgccg acaggtacac caggagcgcg acagcgcccg cgagctgcag acccgccgtg
     2701 ccgtcggtgg tgctgacttg ccacgtcgtt ggctcggttg aaccctgcaa ccacacgcgc
     2761 gagttcaggg tcgtcggcga cgtccccgtt acctgcagct tgacttcgag taccgcgcct
     2821 ggcgtgtagg taaggcccgc gacagtgatc gtcttcaacg tggtcgacgt tccaccgaca
     2881 accgcgacga gtgactcggt caccgatccg cctgcgagga accgcagctt cacctcgtag
     2941 tcagtggtcg cgctgatccg tcggccgata accgacacgt acgttccgcc accgttttgc
     3001 gcttggttaa gcgagacacc aacctgaacc tgcgtatcgg aagacgacac gttggcgagg
     3061 tacgccgcgt cgctcagacc cgcagcagcg actgtgatca ttccggcgct agccgcgacc
     3121 gagaacgcac tcgacttgcc ggtcaatgac caggggccgc caaggtcggc ggtgccgaag
     3181 ccgttggcaa ccgaacggcc gaacccatca gaggcgtatt gggctggagt aacggcggtc
     3241 gacaccgtga tcgggtgggt gacggtgttg gtgcctccgg agttatcggt caccgtcaac
     3301 ccaaccgtgt acgtaccggc gatcgcgtac gtgtgcgacg gcgagacgcc cgttcccgtc
     3361 gtgctgtcgc cgaacgtcca cgcgtaaccg gtgatggtgc catcagtgtc cgtcgaccca
     3421 cccgcgttga acgtcgcgct cgggttggtg gacgtcgacg tgaaagccgc cgtcggcaac
     3481 acattg
//
LOCUS       DATCBJ010000307         3807 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_235243, whole genome shotgun sequence.
ACCESSION   DATCBJ010000307 DATCBJ010000000
VERSION     DATCBJ010000307.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 3807)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3807)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3807
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_235243"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1696)
                     /gene="dxs"
                     /locus_tag="VII50_06155"
     CDS             complement(<1..1696)
                     /gene="dxs"
                     /locus_tag="VII50_06155"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009075594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HEY5050464.1"
                     /translation="MSLLESVEGPDDLKRLDVAQLPQLAEEIRDFLVSAVAKTGGHLG
                     PNLGAVELTIALHRVFNSPTEPIIFDTGHQAYVHKLLTGRREGFAKLRQRGGLSGYPN
                     RDESEHDWVENSHASTALSYADGLAKAFAVRGEKRTVVAVVGDGALTGGMCWEALNNI
                     AAAKDHSVVVLVNDNGRSYSETIGGLAEHLTTLRTNPRYEKALEKVREVLTRTPLVGD
                     PLFDALHGIKRGLKDVLTPQGLFEDLGLKYVGPIDGHDIAAVEAALARARRFGGPVIV
                     HCVTRKGYGYGPAEDDELDHLHGPGAFDPVTGVELPKPASWTGVFSDEIVKIAASRPD
                     IVAITAAMLHPVGLAAFAQAYPNRIVDVGIAEQHAVTSAAGMAMGGLHPVFCVYATFL
                     NRAFDQVLMDVGLHRLPVTFVLDRAGVTGDDGASHNGMWDLSILSLVPGMRIALPRDG
                     ARLRELLGEVVAVDDGPTAIRFPKGALPHDIELVDRIGGLDVLRRDPDADVLIVAFGP
                     MAELALEVAGRLADQGISSTVLDPRWVLPVNPAIAGLAASHSLVVTIEDHGRQGGAG"
     gene            complement(1796..>3807)
                     /gene="acnA"
                     /locus_tag="VII50_06160"
     CDS             complement(1796..>3807)
                     /gene="acnA"
                     /locus_tag="VII50_06160"
                     /EC_number="4.2.1.3"
                     /EC_number="4.2.1.99"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HEY5050465.1"
                     /translation="TDLVLTVTEMLRGHGVVGKFVEFYGPGVTNVPLANRATIGNMSP
                     EYGSTCAIFPIDDETLRYLTFTGRSPERIALVEAYAKEQGLWHDPAAEPDYSERLELD
                     LSTVVPSLAGPKRPQDRVSLSDAKASFRNVLGGYVQKTGGNGSLDEGSAESFPASDPV
                     AIDHEHPGDKPAGVPSAASGANGRLSSRAMVTLDDGTVCEIDHGAVVIAAITSCTNTS
                     NPSVMIGAALVAKNAVEKGLSRKPWVKTTLAPGSKVVTDYYDAAGLTPYLEKLGFNLV
                     GYGCTTCIGNSGPLDDAISKTIADHDIAAAAVLSGNRNFEGRIHPDVKLNYLASPPLV
                     VAYALAGSLDIDITTEPLGIGSDGEPVYLRDIWPSEHEIADVVGQAIQSQMFSDSYGD
                     VFAGDERWQGLQIPEGSIFEWDDVSTYVRKPPYFEGMVRQPAPVTDITDARVLAVLGD
                     SVTTDHISPAGNIKNDSPAGAYLQEHGVLPRDFNSYGSRRGNHEVMIRGTFANIRLRN
                     QLAPGTEGGVTRHLPDGEAMTIYDASTRYIAAGVPLVILAGKEYGSGSSRDWAAKGTA
                     LLGVRVVIAESFERIHRSNLIGMGVLPLQFAAGQTRESLGLTGEETFTIVGLAGADPL
                     PREVTVQAGEVQFRAVVRIDTPGEADYYRHGGIMQFVLRSLLEPV"
BASE COUNT          659 a         1226 c         1281 g          641 t
ORIGIN      
        1 aaccagcgcc gccttggcgt ccgtggtctt cgatcgtcac aacaagtgaa tgggacgccg
       61 caaggccggc gatcgccgga ttgacgggga gtacccaccg cgggtcgagc acagtcgagg
      121 agatgccctg atccgcaagc cggccggcca cctccagggc gagttcggcc atcggtccga
      181 acgccacgat gagcacatcg gcgtccgggt cgcgacgcaa aacgtcgaga ccgccgatgc
      241 ggtcgaccag ctcgatgtcg tgcggcagcg cgcccttcgg gaatcggatt gccgtcggcc
      301 catcgtcaac ggcgaccacc tcgccaagca gctcgcgaag gcgcgcgccg tcacgaggaa
      361 gcgcaatccg catgcccggc accaacgaca ggatcgacaa gtcccacata ccgttgtggc
      421 tggcgccatc gtcgcccgtg acgcccgcgc gatcgagcac gaacgtgacc ggcaggcggt
      481 gcaaaccgac gtccatcaga acttggtcga acgcgcggtt caagaatgtg gcgtagacgc
      541 agaacaccgg gtgcagacca cccatcgcca ttccagccgc cgacgtcact gcatgctgct
      601 cagcgatccc gacgtcgaca atgcgattcg ggtaggcctg tgcgaaagcg gcgagtccga
      661 cggggtgcag cattgccgcg gtgatcgcga cgatgtcggg tcggctagcg gcaatcttga
      721 cgatctcgtc ggagaacact ccggtccagc tggccggctt cggcagctcg acaccggtca
      781 ccgggtcgaa cgcgcctggc ccgtgcaggt ggtcgagttc gtcgtcttcg gcggggccgt
      841 agccataacc cttgcgggta acgcaatgca cgatcaccgg gccgccgaac cggcgggcgc
      901 gggcaagtgc agcctcaaca gcggcgatgt catgcccatc gatgggtccg acgtacttca
      961 agccgagatc ctcgaaaagt ccttgcggcg taaggacatc cttgagacca cgcttgatgc
     1021 cgtgcagtgc atcgaacagc ggatcgccca ccagcggcgt gcgcgtgaga acttcgcgca
     1081 ccttctcgag cgccttctcg taacgcggat tcgtgcgcag ggttgtgagg tgttcggcaa
     1141 gaccaccgat ggtctcggag tacgagcggc cgttgtcgtt gacgagcacg acaaccgagt
     1201 ggtccttcgc tgcggcgatg ttgttgagcg cttcccagca catgccgccg gtgagtgcgc
     1261 cgtcgccgac aacggctact accgttcgct tctcgccgcg caccgcgaat gctttcgcca
     1321 gtccgtcggc ataactgaga gccgtcgaag cgtgcgagtt ctccacccag tcgtgctcgg
     1381 attcatcgcg gttcgggtag cccgacaagc cgccgcgctg gcgcagcttc gcgaagccct
     1441 cccggcgtcc ggtcaacagc ttgtgcacgt aggcctggtg accggtgtcg aagatgatcg
     1501 gctcggtcgg tgagttgaag acgcggtgca gcgcgatggt gagctcgacc gcaccgaggt
     1561 tcggcccgag atgtccgccg gtcttcgcga ccgcactgac caggaagtcg cggatctctt
     1621 cggcaagctg gggcaactgg gcgacgtcga gccgcttgag gtcgtcgggg ccctcgacag
     1681 actcgaggag actcacgtgg accttcacct caccgtgtcg acgcctgagc ggttcacgcc
     1741 tgaaaaccgt tcccgcagtg tagtcgggca tcccggccga cacccagggg tgcagtcaga
     1801 cgggctcgag cagcgaccgc agcacgaact gcatgatgcc gccgtggcgg tagtagtccg
     1861 cctcccccgg cgtatcgatc ctgaccacgg cgcggaactg cacctcgccc gcctgcacgg
     1921 tgacctcgcg cggcagcggg tcggcgcccg cgaggccgac gatggtgaag gtctcctcgc
     1981 cggtgaggcc gagtgactcg cgcgtctggc cggcggcgaa ctgcagtggc aagacgccca
     2041 tgccgatgag gttgctgcgg tgaatgcgct cgaacgactc ggcaatcacg acccgcacgc
     2101 cgagcagcgc ggtgcccttc gccgcccagt cgcgcgagga gccggagccg tactccttgc
     2161 ccgccaggat cacgagcggc accccagcgg cgatgtagcg ggtcgaggcg tcgtagatcg
     2221 tcatggcttc gccgtcgggc aggtggcggg tgaccccgcc ttcggttccg ggggccagct
     2281 ggttgcgcaa ccggatattg gcgaacgtgc cgcggatcat cacctcgtgg ttgccgcgac
     2341 gggacccgta ggagttgaag tcgcgcggca gcaccccgtg ctcctgtaag taggcgccgg
     2401 cgggcgaatc gttcttgatg ttgccggccg gggagatgtg gtcggtggtc accgagtcgc
     2461 caagcaccgc aagaacccgc gcgtcggtga tgtcagtgac gggtgcaggc tgcctgacca
     2521 tgccttcgaa atacggcggc ttgcgcacgt aggtcgacac atcgtcccat tcgaagatgc
     2581 tgccctcggg aatctgcagg ccctgccagc gctcgtcacc cgcaaagaca tcgccgtagc
     2641 tgtcggagaa catctgtgac tggatcgcct ggccgacaac gtcggcgatc tcgtgctcgc
     2701 tcggccagat gtcgcgaagg tagaccggtt caccgtcgga accgatgccg agcggctcgg
     2761 tcgtgatgtc gatgtcgagc gaaccggcca acgcatacgc gacgacgagt ggcggcgagg
     2821 cgagatagtt cagcttcaca tccggatgga tgcgtccctc gaagttgcgg ttgccggaga
     2881 gcacggcggc cgctgcgatg tcgtggtcgg cgatggtctt ggagatggcg tcgtcgagcg
     2941 ggccggagtt accgatgcaa gtcgtgcagc cgtagcccac caggttgaag ccgagcttct
     3001 ccagataggg cgtcagcccg gccgcgtcgt agtagtcggt gaccaccttc gagccggggg
     3061 cgagcgtggt cttgacccag ggcttacgag aaagtccttt ctccacagcg tttttcgcca
     3121 ccagggccgc gccgatcatc acggaagggt tcgacgtgtt cgtacagctg gtgatggcag
     3181 cgatgacaac ggcgccgtgg tcgatctcgc agaccgtgcc gtcgtcgagg gtgaccatcg
     3241 cccggctgct caaccggccg ttcgcgccgc tagccgcgct cggcacgccc gccggcttgt
     3301 cacctgggtg ttcgtgatcg atcgcaaccg ggtcgctcgc ggggaaggat tctgcggagc
     3361 cttcgtcgag cgagccgttg ccaccggttt tctgcacgta gccgccgagc acgttccgaa
     3421 acgatgcctt cgcatccgac agcgatacac gatcctgcgg ccgcttcgga ccggccagtg
     3481 acggcaccac cgtcgagagg tcgagctcga gacgctcgga ataatcgggc tcggcggcgg
     3541 ggtcgtgcca caggccctgt tccttcgcgt acgcctcgac aagcgcgatc cgctcgggcg
     3601 accgaccagt gaacgtgaga tagcgcaggg tctcgtcatc gatcgggaag atggcacagg
     3661 tcgagccgta ttcggggctc atgttgccga tagttgcgcg gttggcgagc ggcacattgg
     3721 tgacgcccgg gccgtagaac tcgacgaact tcccgacgac gccgtgaccg cgcagcatct
     3781 cggtgacggt gagcaccagg tcggtcg
//
LOCUS       DATCBJ010000308         2310 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_152991, whole genome shotgun sequence.
ACCESSION   DATCBJ010000308 DATCBJ010000000
VERSION     DATCBJ010000308.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2310)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2310)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2310
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_152991"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..453
                     /locus_tag="VII50_06165"
     CDS             <1..453
                     /locus_tag="VII50_06165"
                     /inference="COORDINATES: protein motif:HMM:NF024637.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HEY5050466.1"
                     /translation="LATYGIRVTRLGNVTVSLSDEDAEQLRTLARDTAYTALTGSFGA
                     YAQGEALLGAGHGMANGQGGGNEALGIASLGLGAGVLGAFAGTQQAAAPAPAAAPASA
                     APAATAAPSAAALAAPVVAPAFCSHCGHALAAGALFCSACGSAVATAQ"
     gene            467..1123
                     /locus_tag="VII50_06170"
     CDS             467..1123
                     /locus_tag="VII50_06170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5050467.1"
                     /translation="MSVFRSRDNDAARLQSYVFADPTGTGNLGGVGVGGDVDVAAVQR
                     VDATFDPTIFLAGATSLFHDVRAALSAGTIDPVAGRLSPELTSMFGNQLQYAMTTGHQ
                     LTMTSIDQVTATLRGVEAGSAGDIMCLVKYQVVGRMGLVALGDSVPPASQLAAMPQRP
                     WFETWRLTRPAGVTSPPLPATCPSCGAPATGETHCIYCHALLVDATAQFRVEHIECMG
                     "
     regulatory      1181..1264
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01750"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="ZMP/ZTP riboswitches appear to regulate genes
                     involved in one-carbon metabolism; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="5-aminoimidazole-4-carboxamide
                     ribonucleotide (ZMP)/
                     5-aminoimidazole-4-carboxamide-1-beta-D-ribofuranosyl
                     5'-triphosphate (ZMT)"
                     /db_xref="RFAM:RF01750"
     gene            1276..>2310
                     /gene="glyA"
                     /locus_tag="VII50_06175"
     CDS             1276..>2310
                     /gene="glyA"
                     /locus_tag="VII50_06175"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HEY5050468.1"
                     /translation="MPDHLAALDATDPEIAALVRAEETLQTEKIRLIASENYVSEAVL
                     EATGTVLTNKYSEGYPGKRYYEGQQVIDQVETLAINRAKAVFGADHANVQPYSGSPAN
                     LAVYLALANAGDTVMGMALPMGGHLTHGWSVSATGIWFHAVQYGVGRESGRVDMDEVR
                     EIALRERPKIIFCGGTAIPRTIDFPAFAEVAREVDAVLVADIAHIAGLIAGGAHPSPV
                     GYAGVISTTTHKTLRGPRGAMLMCDEQYAKAIDKAVFPGLQGGPHNHTTAAIAVALHE
                     AQQPDFPDYAAAIVTNAQALAEELSSRGFSLVSGGTDNHLILLDLTPQGVAGKPVAKA
                     LDRAGLVVNYN"
BASE COUNT          381 a          803 c          757 g          369 t
ORIGIN      
        1 ctcgcgacct acggaatccg ggtcacccgg ctcggcaacg tcacggtcag cttgtccgac
       61 gaggacgccg agcagctgcg cacgctcgct cgcgacaccg cctacacggc gctgaccggc
      121 tccttcggcg cttacgcgca aggtgaggcg ctcctcggag ccggccacgg gatggccaac
      181 ggccagggtg gcggcaacga ggctcttggc atcgcgtcgc tcggacttgg tgccggcgtt
      241 ctcggcgcgt ttgccggtac tcagcaagca gcggcgcccg cacccgctgc ggcgccggca
      301 tcggctgcac ccgccgctac cgcggcaccc agtgctgctg cgctggccgc accggtcgtc
      361 gcgccagcgt tctgctcaca ctgcggacat gcgctcgccg cgggagcgct gttctgctcg
      421 gcatgcggca gcgcagtcgc caccgcgcag tgagcacgtt gcagctgtga gcgtgttcag
      481 gtcgcgggac aacgacgcag cccgcctgca gtcgtacgtt ttcgcggacc caaccggcac
      541 cgggaacctc ggcggggtcg gagtcggcgg tgacgtcgac gtcgcggctg tccagcgggt
      601 cgacgcgacg ttcgatccga ctatcttcct tgccggtgcg acatcgctct tccatgacgt
      661 ccgcgcggcc ctgagtgccg ggacgatcga ccccgttgcc ggccggctct cccctgaatt
      721 gacctcgatg ttcggcaacc agctgcagta cgcgatgacc acgggtcacc aactgacgat
      781 gacctcgatc gaccaggtga ccgcgacgtt gcgcggcgtc gaggccggca gtgcgggcga
      841 catcatgtgc ttggtcaagt accaggtggt cggacgcatg ggcctggtcg ctctgggtga
      901 cagcgtgccc ccagcgagcc aactcgcggc gatgccccag cgcccgtggt tcgagacctg
      961 gcggctgacc cgaccggccg gcgtaaccag tcccccgctg cccgcgacgt gcccgtcctg
     1021 cggcgccccg gcaacaggtg agacccactg catctattgc cacgccctgc ttgtcgacgc
     1081 gactgcccag ttccgggtcg agcacatcga gtgcatgggc tgaggcagcc caagtgtggg
     1141 ggctgaggca gcccaagtgt gggggctgag gcagctagga tcagtgcgtc gcgactggcg
     1201 catagatggt ggatcaacca ccggggagcg atcgacgcgg acgccgtgcg cctgggtgtc
     1261 caccgaggag acatcgtgcc cgaccacctt gctgcgcttg atgcaactga ccctgagatt
     1321 gccgcgctgg tacgcgccga agagacactc cagaccgaga agatccgcct gatcgcctcg
     1381 gagaactacg tctcggaggc ggtactcgaa gccaccggca ccgtgctcac caacaaatac
     1441 tccgagggct acccgggcaa gcggtactac gagggccagc aggtcatcga ccaagtcgag
     1501 acgctggcga tcaatcgcgc gaaggccgtg ttcggcgccg accacgcgaa cgtgcagccg
     1561 tattcgggct cccctgcaaa cctcgcggtc tatctcgccc tcgccaacgc cggcgacacc
     1621 gtcatgggta tggcactgcc gatgggcggg cacctcactc acggctggtc ggtctccgcg
     1681 accggcatct ggtttcacgc cgtgcagtac ggcgtcggtc gcgaaagcgg acgcgtcgac
     1741 atggacgagg ttcgcgagat cgcgcttcgc gagcggccga agatcatctt ctgcggcggt
     1801 acggcaatcc ctcgcaccat tgactttccg gcgttcgccg aggtcgctcg cgaagtcgac
     1861 gcggtgctgg tcgccgacat cgcacatatc gccggactca tcgccggtgg cgcgcacccg
     1921 tctcctgttg ggtacgcagg tgtcatctcg acgacgacgc acaaaacgct gcgcggccct
     1981 cgaggcgcga tgctgatgtg cgacgagcag tacgccaagg ccatcgacaa agccgttttc
     2041 cctggcctgc aaggcggccc gcacaaccac acgacggccg cgatcgcggt cgcgctgcat
     2101 gaggcgcagc aaccggactt tcccgactac gcagccgcga tcgtcaccaa tgcgcaagca
     2161 ctcgccgagg agttgtcgtc gcgcggcttc tcgctcgtgt cgggcggcac ggacaaccac
     2221 ctgatcctgc tcgacctcac accgcagggc gtagcgggga agccagtcgc caaggctctc
     2281 gaccgtgcgg gcctggttgt caactacaac
//
LOCUS       DATCBJ010000309         2001 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_377799, whole genome shotgun sequence.
ACCESSION   DATCBJ010000309 DATCBJ010000000
VERSION     DATCBJ010000309.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2001)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2001
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_377799"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            269..772
                     /locus_tag="VII50_06180"
     CDS             269..772
                     /locus_tag="VII50_06180"
                     /inference="COORDINATES: protein motif:HMM:TIGR02983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SigE family RNA polymerase sigma factor"
                     /protein_id="HEY5050469.1"
                     /translation="MNRADEDDFRSFVAARSPSLLWFAHILTGDRYAAEDIVQTALAK
                     TAAGWSRVRRKENPEGYVRRAIVNTHLNAVRRRPWREQPREHLPEDIDSRHREHELDD
                     REAMWHALSALPPRQRAVLVLRYYEDLSESDIADVLGCSRGTVKSQAAKGLLHLRRIV
                     QKEDAST"
     gene            769..1980
                     /locus_tag="VII50_06185"
     CDS             769..1980
                     /locus_tag="VII50_06185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5050470.1"
                     /translation="MTDVEQLLRETLADPRHRLEPGPGMYDTVRERARQRRQRTIQIA
                     SACTVVVLIAGVATAIGVNSGHRRAGQVAATPSITPSPPTGQATPIDLGIGSTSAMAV
                     TSSWVFVARTQPDELVKLSTTKLSVTKRAATYDAVDDIAVDAAAGKVWTWSSTQAAAS
                     GSSDGASSTSINEYATSSLAVEGLVGGMPLPAYAFSGAALDGQLWLATSDGLWVVGPD
                     KPGGMGAAKVASGSVFSVAADPVRHRVLYGTAMSIPAGADSLGDIAIYEIDARTHAVI
                     GISTPLPIGKESIAIVGDQIWVGGYGSGSTPRLFHLDGASLEPLHTTGLGAGNPLEFG
                     PGAIVWPGASVVWVRSGGSEALSCVDPQSGALLERWDAVQGPVASMPGHAYAASDGSL
                     DQLSLAGGCTG"
BASE COUNT          333 a          657 c          688 g          323 t
ORIGIN      
        1 gggtactcca gctctgctct gacttgggtg agccgtcggt cgacgtggtg gggacccggt
       61 cgcgacgtct cgggacacga tcgacgaagg gtagagggcc cgcgccgttc ggtgttcgac
      121 cccccattct gccagaccga ccgccacgga cgcacatcgg cgagcttccc ggcgtccaac
      181 ctcaccaggc gggcgggctc gcgggcaacc cgaatacgcg agagcgtgtc cttaggtgca
      241 tgaccgccct gcggacagga gcgagccggt gaatcgcgcc gacgaggacg acttcaggtc
      301 gttcgtcgcg gcgcggtcgc cctcgttgct gtggtttgcc cacatcctca cgggcgatcg
      361 ttacgcggcc gaagacatcg tccagaccgc cctggccaag acggcagcag gctggtcgcg
      421 cgtgcgccgc aaggagaacc cggaagggta cgtacggcgc gcgatcgtga acacgcacct
      481 gaatgcggtg cggcgccggc catggcgaga acaaccgcga gaacaccttc ccgaggacat
      541 cgactcccgc catcgcgagc acgaactcga cgaccgcgag gcgatgtggc atgcgctctc
      601 ggcgttaccg ccacggcagc gtgcagtcct cgtcctgcgc tactacgagg acctctcgga
      661 gtccgacatc gccgacgtac tcggatgcag tcgcggcacc gtgaaaagcc aggccgcgaa
      721 ggggttgctg catcttcggc ggatcgtgca gaaggaggac gcgtcaacat gacggacgtc
      781 gagcagttgc tgcgagagac gttggctgat ccacgtcacc ggctcgagcc cggtcctggg
      841 atgtacgaca ccgtgcgtga gcgggcgcgt caacgacggc agcgaaccat ccagatcgcc
      901 agcgcgtgca ccgtggtcgt cctcatcgcc ggtgtggcga ctgcgatcgg agtcaactcg
      961 gggcatcgcc gcgctggcca ggtcgcggcg acgccgtcga tcaccccgtc gccacctacg
     1021 ggccaggcaa caccgatcga cctgggtatc ggcagcacca gcgcgatggc tgttacgtca
     1081 tcgtgggtct tcgtcgcgcg aacgcagccc gacgagctgg tgaaactgtc gacgaccaag
     1141 ctttctgtca ccaaacgagc tgcgacctat gacgcagttg acgacatagc cgttgacgcc
     1201 gccgcgggca aggtgtggac ctggtcttca acgcaggccg ccgcatcggg gtcatcggac
     1261 ggggcgagct cgacgagcat caacgagtac gcgacgtcaa gtctcgctgt cgagggtctc
     1321 gtaggtggga tgccactgcc ggcctacgca ttcagcggag ccgcgttgga tggccagctt
     1381 tggctcgcga catccgatgg cctctgggtt gttggccccg acaagcccgg ggggatgggg
     1441 gccgcgaaag tcgccagcgg ctcggtgttt tcggtcgccg ctgaccccgt acgtcaccga
     1501 gtcctgtacg gcactgcgat gagcattccg gctggggcag actccttggg cgacatcgcg
     1561 atttacgaga tcgacgcgcg cacccacgcc gtgatcggca ttagcacgcc gctcccgatc
     1621 ggcaaggagt cgatcgcgat cgtcggcgat cagatctggg tcggcggcta cgggtcgggt
     1681 tccacccccc gcttgttcca cctcgacggg gcgtcgctgg aacctctgca cacgaccggc
     1741 ctcggcgcgg gcaacccgct cgaattcggc ccgggtgcga tcgtctggcc aggtgcgtcg
     1801 gtggtgtggg tacggtcggg cggcagcgaa gcgctgtcgt gcgtcgaccc gcaatcaggc
     1861 gccctgctcg agcgctggga cgccgtccag ggtccggtgg cctcgatgcc ggggcacgcg
     1921 tacgcggcca gcgacggctc gctcgaccag ctgagtctgg cgggaggctg tacgggttaa
     1981 cgcactactt gcctacggcg c
//
LOCUS       DATCBJ010000310         4681 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_95899, whole genome shotgun sequence.
ACCESSION   DATCBJ010000310 DATCBJ010000000
VERSION     DATCBJ010000310.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 4681)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4681)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4681
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_95899"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..374)
                     /locus_tag="VII50_06190"
     CDS             complement(<1..374)
                     /locus_tag="VII50_06190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011718973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEY5050471.1"
                     /translation="MVAERPAGQPLQAVPANGVPTNGVPTNGVPTNGVHRGAVGAAAG
                     SESPRVEHRPTVWGVAALAGVSKTTVSRVLTGSPRVSPQARIAVERAIEKLNYVPNRA
                     ARALVTRRADTLALIVSEPETRL"
     gene            537..1730
                     /gene="xylA"
                     /locus_tag="VII50_06195"
     CDS             537..1730
                     /gene="xylA"
                     /locus_tag="VII50_06195"
                     /EC_number="5.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009045 - xylose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0042732 - D-xylose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylose isomerase"
                     /protein_id="HEY5050472.1"
                     /translation="MPSLLRPTPQDRFTFGLWTVGWQARDPFGDPTRPALDPVETVHR
                     LAELGAYGVTFHDDDLIPFGSSAADRDKQIARFKDALAETGLVVPMATTNLFTHPVFK
                     DGGFTSNDRAIRRYALRKVMRNLDLAVELGAKTYVFWGGREGAETDSAKDVRSALARY
                     AEGIDMLAQYAIDNGYDIRFAIEPKPNEPRGDILLPTVGAALAFISTLEHHEMVGVNP
                     EVGHEQMAGLNFVHSIAQALWQGKLFHIDLNGQRGIKYDQDLMFGHGDLLNAFFLVDL
                     LENGGIVGARAYDGPRHFDYKPLRTEDIDGVWASAAANMSTYLLLKERAIAFRADPEV
                     QAALVAARVAELAIPTLGEGESYTNLLEDRSAFEDFDVDAAGKRGYHFTQLDQLAVEH
                     VLGAR"
     gene            1746..3161
                     /locus_tag="VII50_06200"
     CDS             1746..3161
                     /locus_tag="VII50_06200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HEY5050473.1"
                     /translation="MTLVAGVDSSTQSCKVVIRDADTGALVREGRAAHPDGTEVDPQA
                     WWTALGSALQEAGGLDGVAGLSVAAQQHGMVCLDAAGAVVRPALLWNDTRSAPDASAL
                     VSELGDGDWLTGAQVWASAVGSVPVASFTVTKLRWLARVEPENAVRTAAVCLPHDWLT
                     WRLAGNGAPDLATLATDRSDASGTGYWSPSAGEYRDDLLHGAFGFVPHLPRVLGPAES
                     AGITSRGVRLGAGAGDNAGAALGLGARRGDVIVSIGTSGTVFGVSATPTADAAGEVAG
                     FADASGHFLPLVCTLNAARVLDATAAMLGVDAQTLAELALSAPPGADGLVVVPYLEGE
                     RTPNRPNASGTVHGWRLGNSTPAHLARASVEGVLCGLADGLDALVAQGFAVERILLIG
                     GGARSAAIRSIAPAVLGRPVVVPPAGEYVADGAARQAAWMASGATDPPVWESPNTQTF
                     DAPAALWVRARYAEVRDLIPS"
     gene            complement(3196..3501)
                     /locus_tag="VII50_06205"
     CDS             complement(3196..3501)
                     /locus_tag="VII50_06205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5050474.1"
                     /translation="MSDPRGRHARPRRGKLTAPTRETAPAGNVSVIVAQLNTARCKWC
                     NDDLEHCHDSLVRHTAGDQHCMDPQCVVPVEAHHMVIACTDFGCGCALSAPGTAATA"
     gene            3682..3963
                     /locus_tag="VII50_06210"
     CDS             3682..3963
                     /locus_tag="VII50_06210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5050475.1"
                     /translation="MTLLDKAKVAAEKAAARGKQGVQQGQAKIDAIQTKRKAEALLRE
                     LGAAYYAELRHGGSHDAVETAVDALDSHEKEHGAVEDEASDSDTGESSE"
     gene            4075..4245
                     /locus_tag="VII50_06215"
     CDS             4075..4245
                     /locus_tag="VII50_06215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5050476.1"
                     /translation="MEQSHHDVGAMIQISADQCRRAALAVCGAAADAADAQLLLDALG
                     LRQILTTISIPA"
BASE COUNT          734 a         1541 c         1620 g          786 t
ORIGIN      
        1 agtcgggtct ccggctcgga aacgatcaga gccagcgtgt cggcgcgacg cgtcacgagc
       61 gcccgggcgg ctcggtttgg aacgtagttg agtttctcga ttgctctctc tacggcgatg
      121 cgggcttgcg gcgatacccg aggcgacccg gtcagcactc gcgagacggt ggtcttggag
      181 acccccgcca acgccgcgac gccccacacc gtcggccgat gttcgacgcg cggcgattcc
      241 gagccggcgg ccgcgcccac agcgccacga tgcacgccgt tcgtgggcac gccgttcgtg
      301 ggcacgccgt ttgtgggcac gccgttcgca ggcaccgcct gtagcggctg cccggccggg
      361 cgctcggcga ccatacggtg atgatttcac aggaggcagg tacgccgccg ggtgagaact
      421 gggccggcgc tgcgaacgtt cccgcttttc gttgaccggc cgccgtccgg ccaaatatgt
      481 tgagtgagca aatatgttga gctggccaac aaatcgatct cgccaaggag agttccgtgc
      541 cgtcgttgct gcgcccgacg cctcaagacc gcttcacctt cgggctctgg acagtgggct
      601 ggcaggcgcg cgaccccttc ggcgacccga cccggcccgc gctcgacccg gtcgagactg
      661 tgcaccgtct tgccgaactc ggcgcctacg gcgtgacctt ccacgacgat gacctgattc
      721 cctttgggag cagtgcagcc gatcgtgaca agcagatcgc tcgcttcaag gacgcgctcg
      781 ccgaaaccgg cctcgtcgta ccgatggcga ccaccaacct gttcacccat ccggtgttca
      841 aggacggcgg cttcaccagc aacgaccgcg cgatacgccg ctatgcactg cgcaaggtca
      901 tgcgcaacct cgaccttgcg gtcgagcttg gcgcgaagac ctacgtgttc tggggtgggc
      961 gtgaaggcgc cgagaccgac tctgcgaagg acgtcaggtc ggcgctggca cgttatgccg
     1021 agggcatcga catgctcgcg cagtacgcga tcgacaacgg ctatgacatt cggttcgcca
     1081 tcgagccgaa gcccaacgaa cctcgcggcg acatcttgct gccgaccgtc ggcgcggcgt
     1141 tggcgttcat ctcgacactt gagcaccacg aaatggtggg cgtgaacccc gaggtgggtc
     1201 acgagcagat ggctggcctc aacttcgtgc acagcatcgc gcaggcgttg tggcagggga
     1261 aacttttcca catcgacctc aacgggcagc gcggcatcaa atacgaccag gacctcatgt
     1321 ttggtcacgg cgacctgctc aatgccttct ttctcgttga cctgcttgag aacggtggca
     1381 tcgtcggcgc tcgcgcatac gatggcccgc gtcatttcga ctacaagccg ctgcgcactg
     1441 aagacattga cggtgtctgg gcatccgccg cggcgaacat gtcgacctac ctgttgttga
     1501 aggagcgagc aattgcattc cgggccgacc cggaggtgca ggccgctctg gtcgccgcac
     1561 gggtggccga gttggccatc ccgaccttgg gtgagggaga gtcgtacacg aatcttcttg
     1621 aagaccgctc ggcgttcgag gacttcgacg tcgacgccgc aggcaagcgc gggtatcact
     1681 tcacccaact cgaccagctt gcggtcgagc acgtgctcgg cgcgcgctga cctgacgcgg
     1741 tcgacgtgac gctcgtcgcc ggggtcgact cctcgacgca gtcatgcaag gtcgtcatcc
     1801 gtgacgccga caccggcgcc ttggtgcgcg agggtcgcgc cgcgcatccc gatggcactg
     1861 aggtcgatcc gcaggcgtgg tggaccgcgc ttggttccgc gctgcaagaa gcaggcggtc
     1921 tcgacggcgt cgcagggttg tccgtcgcag cgcaacaaca cggcatggtg tgtctcgacg
     1981 cggccggcgc cgtcgtccgg cccgcactgc tgtggaacga cacgaggtcg gcccccgatg
     2041 cctcggcgtt ggtatccgag ctaggagacg gggattggct gaccggcgcg caggtgtggg
     2101 cgtcggcggt cggctccgta cccgtggcgt cgttcacggt gaccaagctg cggtggctcg
     2161 ccagggtcga gcctgagaac gccgtgcgca ccgcggccgt gtgcctgccg cacgactggc
     2221 tgacctggcg ccttgccgga aatggcgcgc ctgatctggc gacgcttgcc accgaccgca
     2281 gtgacgcgag tggcactggc tactggtcgc cgtccgccgg tgaatatcgc gatgacctct
     2341 tgcacggagc attcgggttc gtgccccacc tgccgcgggt tcttggtccc gccgagtcgg
     2401 cgggcatcac ttcgcgcggc gtccgcctcg gcgccggcgc gggcgacaac gccggggcgg
     2461 cgctcggtct tggtgcgcgc cgtggcgatg tgatcgtctc gatcggcacg tcgggcacgg
     2521 tattcggcgt cagcgcgaca cctaccgcgg acgccgcagg ggaggttgcg ggtttcgctg
     2581 acgcaagcgg gcacttcctg ccgttggtgt gcacgctgaa tgccgctcgt gttctcgacg
     2641 ccaccgcggc gatgctcggc gtcgatgcgc aaaccctcgc cgaacttgcg ctatcggcgc
     2701 caccgggagc cgacggtctc gtcgtcgtgc cctacctcga aggcgagcgg acgccgaaca
     2761 ggccgaacgc gagcggcact gttcacggct ggcggctcgg caactcgacc cccgcccatc
     2821 tggcgagggc cagcgtcgaa ggcgtcctgt gcggacttgc cgacgggctc gacgcgctcg
     2881 tcgcccaggg attcgcggtc gaacgcatcc tgctcatcgg cggtggcgca cgctctgcag
     2941 cgatccgtag catcgcgccg gcggtgctcg gccggcccgt ggtcgtccca ccggccggtg
     3001 aatacgtcgc tgacggcgcc gcacgacagg cagcatggat ggccagtggc gcaacggatc
     3061 cgccggtgtg ggagtcgccg aacacccaga ccttcgacgc gccggccgca ctgtgggtgc
     3121 gcgcccgtta cgccgaggtc cgcgacctca tccccagcta gctgcggcct cgcacctacg
     3181 gtgcctgcca aactgctagg ccgtggcggc ggtgccggga gcgctcaacg cgcatccgca
     3241 gccgaagtcg gtgcacgcaa tgaccatgtg atgagcctcg accgggacca cgcactgcgg
     3301 atccatgcag tgctggtcgc ctgcggtgtg gcgcacgagc gagtcatggc agtgctcaag
     3361 atcgtcgttg caccacttgc agcgggcggt gttgagctgc gccacgatca ccgagacgtt
     3421 gcccgcgggc gcagtctctc gagtcggcgc tgtcaacttg cccctgcgcg gccgtgcgtg
     3481 ccggcctctc gggtcgctca tgggtacgaa ggtaggcgcg aaacgcggca attgcgtcgc
     3541 gacgcgcccg accgaacgtt cgcgcccagc cgccgatcgg acgaggttac cggccttcgc
     3601 ggtctcggtc cgccagcggt aacgtggctc ctccagccaa aggacccacg atccaaggga
     3661 cctctaaaca agggcaacgt catgacgctg ttggacaagg cgaaggttgc cgcagagaag
     3721 gcggcggctc gcggtaagca gggtgtgcag cagggccagg ccaagattga cgcgatccag
     3781 accaagcgga aggcggaggc gttgctgcgc gaactcggcg ccgcgtatta cgccgagctg
     3841 cgtcacggtg gcagccacga cgccgtcgag acggcggtcg atgcgctcga cagccacgag
     3901 aaggagcacg gcgcggtcga ggacgaggcg agcgactcgg acaccggcga gagcagcgag
     3961 tagcagcacg ttagaacgcg ggcggcgcgg gcacatcagt gttcaccgag acatcagtgc
     4021 tgaccaggca ccgccgcagc gggcgtcacg gcaggcatca gcaggaggac gcttatggag
     4081 cagtcacatc acgatgttgg ggcgatgatt caaatctccg ccgaccaatg cagacgggcg
     4141 gcgctcgcgg tatgcggcgc cgccgcggac gcggcagacg cgcagttact ccttgatgcc
     4201 ctcgggttaa ggcaaatact gaccaccatc tcgatcccgg cctgacccgt tggcggtcgt
     4261 cggcggcggc tagctgtcgt cgtccgaccg ggccggccgc gatggttgct gcggccgagg
     4321 tggctggctt cgcatgggcc gttgcggcgc gggttgcacc cgcacattcg actgcacgat
     4381 tggcttggtc gccggattgg gcgtcgccgg attgagcgtc ggcggattga gcctcggcgg
     4441 tctggcggtt gatgtagcca ctcgcggggt ccgctgtctg acgttggtcg gatccgagac
     4501 accggtcgga tccgatgcct tgaccttcgc aaccgttgtc ggcttcggtc gtgccgcggg
     4561 tcgctttcgt ggagctgttg gctcgacggt cggggcagcc gaggttgtcg acgtcgaccg
     4621 tggcggcggg accccggccg gggcggccgc cggggtggtc cgctcgccga cgtcgtcccc
     4681 t
//
LOCUS       DATCBJ010000311         3437 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_228306, whole genome shotgun sequence.
ACCESSION   DATCBJ010000311 DATCBJ010000000
VERSION     DATCBJ010000311.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 3437)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3437)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3437
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_228306"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..715)
                     /locus_tag="VII50_06220"
     CDS             complement(<1..715)
                     /locus_tag="VII50_06220"
                     /inference="COORDINATES: protein motif:HMM:NF016047.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding repeat-containing protein"
                     /protein_id="HEY5050477.1"
                     /translation="MDLGAWVYPEVVQMSSIPRALQLLSSGVAALTVGAGLVAASSGA
                     AAAAVAQSAAVAAPAPSVLPAVLPSNVQRISGATRFDTAIATSQDQFAVSGSAAAVVL
                     TRADTYPDALAGVPLAAKVGGPLLLTSSTSLSAAVRAEIIRVLPAGGPVYILGGTSAV
                     SAAVATTITGLGFVVHRLAGANRFATAVAVAGALGNPTTVFEATGLNFPDALAGGPAA
                     IEAGAAILLTNGSAPAAETT"
     gene            930..2804
                     /gene="dnaK"
                     /locus_tag="VII50_06225"
     CDS             930..2804
                     /gene="dnaK"
                     /locus_tag="VII50_06225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HEY5050478.1"
                     /translation="MARAVGIDLGTTNSVVAVLEGGEPTVIANAEGSRTTPSVVAFAK
                     NGEVLVGEVAKRQAVTNADRTIRSVKRHIGTDWSVAIDGKNYTSQEISARILQKLKRD
                     AEAYLGEQITDAVITVPAYFNDSQREATKEAGTIAGLNVLRIINEPTAAALAYSLDKG
                     EKEQTILVFDLGGGTFDVSLLEIGEGIVEVKSTSGDTHLGGDDWDQRLVDYLVTAFKN
                     SNGIDLSKDKMALQRLREAAEKAKIELSQQAETTVNLPYITASAEGPLHLEVKVTRSE
                     FQKMTSDLLDRTKGPFHQAIKDAGIKVDQIDHVVLVGGSTRMPAIVDLVRELTGGKEP
                     NKGVNPDEVVAVGASLQAGVMKGEVKDVLLLDVTPLSLGIETKGGVFTKLIERNTTIP
                     TKRSETFTTADDNQPSVQIQVYQGEREMAAYNKKLGMFELTGLPPAPRGIPQIEVTFD
                     IDANGIVHVSAKDLGTGKEQSMTITGGSALPKEDIERMMREAESHAEEDRARKEEAET
                     RNQAETLVYQTEKFLRENTDSVPAPEKDAVDTALANLKKALEGTDIGAVRTATDELAA
                     ESQKMGSAIYANAQANSAAAGHDGHEGGESGPTSGATDGANDGADEVIDAEIVDEGTP
                     "
     gene            2801..>3437
                     /gene="grpE"
                     /locus_tag="VII50_06230"
     CDS             2801..>3437
                     /gene="grpE"
                     /locus_tag="VII50_06230"
                     /inference="COORDINATES: protein motif:HMM:NF013212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HEY5050479.1"
                     /translation="MSGSDQYESPLEPVVRDKRRVHFDPTSGAVSHDPSGARSTDGPA
                     SAGPSGPASPGIDSIELSQLRTELAERTADLQRVHAEYANYRKRVERDREAVREFAVG
                     DALAALLPVLDDIGRAREHGELEGGFRQVAEALDAAVTKLGLTRFGQPGDPFDPTQHE
                     ALMHAYSDEVTQPTAVQILQPGYSYAGRVVRPARVAVAEPTVSLPVDLSVED"
BASE COUNT          670 a         1166 c         1123 g          478 t
ORIGIN      
        1 ccgtcgtctc agccgccggt gctgagccgt tcgtgagcag gatcgcggca cccgcctcga
       61 tcgcggccgg acctcccgcc agtgcgtcag ggaagttcaa gccggtggcc tcgaacacag
      121 tcgtggggtt accgagcgcg ccggcgaccg cgaccgccgt cgcaaaccgg ttcgcgccgg
      181 cgagtcgatg gaccacgaac ccgaggccgg tgatcgtcgt cgcgaccgcg gcgctgaccg
      241 ccgacgtccc accgaggatg tagaccggcc cgcctgcggg caacactcgg atgatctcgg
      301 cgcgcaccgc cgcgctcaag gaggtcgacg atgtcaacag cagcgggccg cccaccttgg
      361 ccgccagcgg cacgccggcc agcgcgtcag ggtaggtatc ggcgcgggta agcacgacgg
      421 ccgcggcgct gccggagaca gcgaactggt cctgtgacgt ggcgatcgcg gtgtcgaaac
      481 gagtcgcgcc agagatgcgc tgcacgttcg agggtaggac ggcagggagg accgacggcg
      541 caggcgcggc aacggctgcc gactgcgcga cggcagcagc ggccgcgcca ctcgatgccg
      601 ccacgagccc ggcgcccacg gtgagcgcgg cgactccgct gctcaacagc tgcagcgcac
      661 gtggaatcga cgacatctgc acgacctccg gatagaccca ggcgcccaaa tccatgaccg
      721 cttcggctcc ccgaggctag tgggcgacgc ccggggaaac taccccaccg gtctgcacct
      781 atgccgatga tcgccaggac cggccggccg gccacggctc ttttggcccg gtttctagag
      841 ttgagcctgc aggactcaac ttcggcttga cttccgccgc cgcgccacta acatgagtgg
      901 tacagactca agtttcgaag gagaccccca tggcacgagc ggtcggcatc gaccttggca
      961 cgacgaactc cgtcgtcgcc gttcttgaag gcggcgagcc cacggtcatc gcgaacgccg
     1021 agggatcgcg caccacgccg tccgtcgtcg cgttcgccaa gaatggtgag gtcctagtcg
     1081 gtgaggtcgc caagcggcag gccgtcacca acgcagaccg caccatccgg tcagtcaagc
     1141 gccacattgg cactgactgg tccgtcgcga tcgacggcaa gaactacacc tcgcaggaga
     1201 tcagcgcacg catcctgcaa aagctgaagc gggacgccga ggcctacctc ggcgagcaga
     1261 tcaccgacgc ggtgatcacc gtgcccgcct atttcaacga ctcccagcgc gaggccacca
     1321 aggaagccgg caccatcgcc ggcttgaacg tacttcgcat catcaacgag cccactgcgg
     1381 ccgcgcttgc ctacagcctc gacaagggtg agaaggagca gacgatcctg gtcttcgacc
     1441 tcggcggcgg caccttcgac gtctccctgc tcgagatcgg cgagggcatc gtcgaggtca
     1501 agtcgaccag cggcgacact cacctcggtg gcgacgactg ggaccagcgc ctcgtcgact
     1561 atctcgtgac ggcgttcaag aacagcaacg gcatcgacct gtccaaggac aagatggcgc
     1621 tgcagcgact tcgcgaggcc gcggagaagg ccaagatcga gctctcgcag caggccgaga
     1681 ccacggtcaa cctgccgtac atcaccgcga gtgccgaagg cccactgcac ctcgaggtca
     1741 aggtcacccg ctcggagttc cagaagatga cctccgatct gctcgaccgc accaagggtc
     1801 ccttccacca ggccatcaag gacgccggca tcaaggtcga ccagatcgac cacgtcgtgc
     1861 tggtcggtgg ctcgacccgg atgcccgcga tcgtcgacct ggtccgcgag ctaacgggcg
     1921 gcaaggagcc aaacaagggc gtcaaccctg acgaggtcgt cgccgtcggc gcgagcttgc
     1981 aggcgggcgt catgaagggc gaggtcaagg acgttctgct cctcgacgtc accccgctgt
     2041 cgctcggcat cgagaccaag ggtggtgtgt tcaccaagct gatcgagcgc aacaccacga
     2101 tcccgacgaa gcgatccgag accttcacca cggctgacga caatcaaccg agcgtgcaga
     2161 tccaggtcta ccaaggcgag cgcgaaatgg ctgcctacaa caagaagctc ggcatgttcg
     2221 aactgaccgg cctgccaccg gcgccgcgcg gaatcccgca gatcgaggtc accttcgaca
     2281 tcgacgccaa cggcatcgtg catgtgtccg cgaaggacct cggcacgggc aaggagcagt
     2341 cgatgacgat caccggtggc tccgcactgc cgaaggaaga catcgagcgg atgatgcgcg
     2401 aggccgaatc gcacgccgag gaggaccgcg cccgcaagga agaggccgag acccgcaatc
     2461 aggccgagac actcgtctac cagaccgaga aattccttcg ggagaacacc gattcggtcc
     2521 ccgctcctga gaaggacgcc gtcgacaccg cgttggccaa cttgaagaag gcactcgagg
     2581 gcacggacat cggcgcggtt cgcaccgcca ccgatgaact ggcggccgag agtcagaaga
     2641 tgggcagcgc gatctacgcg aacgcgcagg caaatagtgc agctgctggt cacgatggtc
     2701 acgagggtgg cgagagcggc ccgaccagcg gcgcaacgga cggtgcaaac gacggcgccg
     2761 atgaggtgat tgacgccgag atcgtcgacg agggcacccc gtgagcggct ccgaccagta
     2821 cgagtcgccg ctggagcctg tggttcgcga caagcgccga gtgcatttcg acccgacgtc
     2881 cggcgcggtc tcgcacgatc cgtccggcgc gaggtcaacg gatgggcccg cttcggcggg
     2941 cccatccggg cctgcttcac cgggcatcga ctcgatcgag ctgtcgcagc tacgcaccga
     3001 gctagccgaa cgaaccgccg atctccaacg cgtgcacgcc gagtacgcca actaccgcaa
     3061 gcgcgtcgaa cgtgaccgcg aagccgtgcg ggagttcgcc gtcggtgacg cactggcggc
     3121 gttgctcccg gtcctcgatg acatcggccg ggcgcgcgag catggcgagc tcgagggtgg
     3181 attccggcag gttgccgagg ctctggatgc cgccgtcacc aagctcgggc tgacccgctt
     3241 tgggcaaccg ggcgacccgt tcgacccgac gcagcacgag gcattgatgc acgcgtactc
     3301 cgacgaggtc acgcaaccga ctgctgtgca gattttgcag cccggctaca gctacgcggg
     3361 tcgcgtcgtg cgtcccgcgc gggtcgcggt ggccgagccc acagtgtcgc tgccggtcga
     3421 tctttcggtc gaggacg
//
LOCUS       DATCBJ010000312         3441 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_386117, whole genome shotgun sequence.
ACCESSION   DATCBJ010000312 DATCBJ010000000
VERSION     DATCBJ010000312.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 3441)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3441)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3441
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_386117"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..397
                     /locus_tag="VII50_06235"
     CDS             <1..397
                     /locus_tag="VII50_06235"
                     /inference="COORDINATES: protein motif:HMM:NF039482.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5050480.1"
                     /translation="AAGGPIVSPHMDALIFTPVAPHMAFDRSLVLDAKCERVAVRVLE
                     RSGQVAVSVDGQLRGVLDPGDWVSVYAGPKRAKLARLTELDFLGRVRERFALADAAAA
                     LADGTAPPIYDPGEPLPEDLAHPGPPDIR"
     gene            complement(409..1773)
                     /locus_tag="VII50_06240"
     CDS             complement(409..1773)
                     /locus_tag="VII50_06240"
                     /inference="COORDINATES: protein motif:HMM:NF022517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2867 domain-containing protein"
                     /protein_id="HEY5050481.1"
                     /translation="MVGANGFVGRKLTVGLVEADYDVVALSRRVPEIAGATGRAVDVA
                     DEADVTVALHGCEVAFYLVHSLSDEDFRARDRQLAESFGRAAAAAGLQRIVYLGGLGH
                     DPESEHLVSRQEVGTALASAGIPVVELRAAVVLGAGSISFEMLRYLTERLPFMVCPRW
                     VRTAIQPIAWADMIRYLIGAIDVAPGVYEVGGADVTSYREMITAYATARGLRRRRIVD
                     VPFLTPRLSSYWLDLVTPVDRRVSHALIESLVTEVVVEDHERTDAAFNIKPMGLVAAL
                     SAALDDQALELDHDLLSRDGGLRDGVYTVRVTVPLSGSESTRLDADLGRIGGSYSWYG
                     LTFAWKVRALLGRVAREHWTLGLPQEIVQGATVDWWQVARREPGILVLRATHWFPGEA
                     WLGYQCTDNELTQVGSLRPKGIPGFLYWKVLQPVHRQVFRALARHRVARSITQEDRVL
                     RTEG"
     gene            1918..2442
                     /locus_tag="VII50_06245"
     CDS             1918..2442
                     /locus_tag="VII50_06245"
                     /inference="COORDINATES: protein motif:HMM:NF015513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEY5050482.1"
                     /translation="MQQEIVLAMLAKEPSRAFQLRSRLDDALGPLAEGLNAGHIYVTL
                     GRLEKAGLVAVVGADAAERADTKVYRLTPAGHERVTEWLSEVSSPKPDLAEFHLKLIA
                     AAAAGLADPISIVDLQRRELLRRLRDVQQAAMAEPNQSDAGLVLEGIVLRLQADLRWL
                     ESCERVWSGRGSKR"
     gene            2439..3176
                     /locus_tag="VII50_06250"
     CDS             2439..3176
                     /locus_tag="VII50_06250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009071988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY5050483.1"
                     /translation="MNESVLLRARGLRKLYGSGEGLVRAVDDVDLDVIRGEMLAVMGP
                     SGCGKSTLLHLLGGLDRPTAGELSLDGRRIDQLSERALAQLRRHEVGFVFQAYHLMDE
                     LTAQENIELPALLAGRAPRDAQRRARQLLEQVGLADRADHLPSALSGGQRQRVAIARA
                     LANEPLVVLADEPTGNLDSAATLEVLRLFEVLHNDGLTLVIVTHDERIAATADRLISM
                     RDGALVDETRLVDSARRNLADLAGLET"
     gene            3177..>3441
                     /locus_tag="VII50_06255"
     CDS             3177..>3441
                     /locus_tag="VII50_06255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5050484.1"
                     /translation="MKGRVFLVFRLAVKDLRHRPAQALLLLLAIAAGTTTLTLGLALH
                     GTTNNPYARTRAATNGPDVVATVMPGGSSAPAPAITATPGGAVE"
BASE COUNT          626 a         1124 c         1108 g          583 t
ORIGIN      
        1 cgccgccggc ggtcccatcg tgtctccgca catggatgcg ttgatcttca cacctgtagc
       61 ccctcatatg gcgttcgaca gaagcctcgt gctcgatgcg aagtgcgagc gcgtcgccgt
      121 ccgcgtgctg gagcgatccg ggcaggtcgc cgtcagtgtc gacgggcaac tacgcggcgt
      181 cctcgacccc ggcgattggg tgagcgtgta cgccggcccc aaacgggcga agctggcgag
      241 gctgacggaa ctcgacttcc tgggcagagt ccgcgagcgg ttcgcgcttg ccgacgcggc
      301 ggccgcacta gccgatggaa cggcaccgcc gatctatgac cctggagagc cactacccga
      361 agacctcgcc caccctgggc cacccgacat ccggtaaccc gaccacgctt atccttcggt
      421 gcggagcact cgatcctctt gggttattga acgcgccacc ctgtgtcgag caagcgcccg
      481 gaagacttgg cggtgcacag gttggagcac tttccagtag aggaagcccg ggattccctt
      541 cgggcgaagc gacccgacct gggtcagctc gttgtccgtg cactgatagc cgagccatgc
      601 ttccccggga aaccagtgag tcgcgcgcag tacgaggatg ccgggctcac gtcgcgcgac
      661 ctgccaccaa tccacggtcg cgccttggac gatttcctgt ggcaggccga gcgtccagtg
      721 ctcccgcgcg acgcggccga ggagcgcacg caccttccac gcgaacgtga ggccgtacca
      781 gctgtagctt ccgccgatcc gaccaagatc ggcgtcgagg cgtgttgatt cactgccgga
      841 aagggggaca gtcacgcgga cggtgtagac accgtctcga aggccgccgt cgcggctgag
      901 taagtcgtga tcgagctcga gcgcctgatc gtcgagcgcg gcggagagcg cagcgacgag
      961 gcccatcggc ttgatgttga aggccgcgtc cgttcgctcg tgatcctcga cgacaacttc
     1021 ggtcaccaga ctctcgatga gcgcgtggct gacacggcga tccaccgggg tgacgagatc
     1081 cagccagtac gacgacagtc gaggcgtcag gaatggcacg tcgacgatgc gtcgacgccg
     1141 caggccgcgc gcagttgcgt aagcagtaat catttcgcga tacgacgtga cgtcggcgcc
     1201 gccgacctcg tacacgccag gagcgacgtc gatcgctccg atcagatagc ggatcatgtc
     1261 ggcccaggcg atcggctgaa tcgcggtgcg tacccagcgt gggcagacca tgaacggaag
     1321 ccgctcggtg agatatcgaa gcatctcgaa ggagatgctg cccgcgccga gcacgaccgc
     1381 ggctcggagt tcgacaacgg gaattcctgc actcgcgagc gccgtgccga cctcctggcg
     1441 gctgacaaga tgctcggact cggggtcgtg ccccaggccg ccgaggtaga caatgcgttg
     1501 gaggccagcc gctgcggcag ctcgcccgaa gctttccgcc aactgtcggt cgcgagcgcg
     1561 aaagtcttca tcacttaacg aatgcacgag atagaaggcg acctcacacc cgtgcagtgc
     1621 gaccgttacg tcggcctcgt cggcgacgtc aactgctcgt cctgtggcgc cagcgatctc
     1681 gggaacgcgg cgcgacagtg cgacgacgtc gtagtcggcc tcgactagac caaccgtgag
     1741 cttccgcccg acgaacccat ttgctcccac cacagcgacc ctcacacctc caccatgccc
     1801 aagaccgatt cgtctcatgt cgcgtgtcgt cggtcagcgc tcaccccacg cagtccgccc
     1861 ttgaccatgg ttcacctaac ggttaggttt ttgtctaacc gttagatcgg atgacacatg
     1921 cagcaggaaa tcgtgctggc gatgctggca aaggagcctt cgagggcttt ccagctgcgg
     1981 tcgcgtctcg atgacgcgct aggaccgctc gccgaaggcc tcaatgccgg tcacatctac
     2041 gtgactctcg gccggctcga gaaggccgga ctggttgcgg tagtcggggc cgatgcagcc
     2101 gagcgcgccg acaccaaggt gtatcgactc acgccggcag gccacgaacg cgtcaccgag
     2161 tggctaagcg aagtgagctc gccgaagcca gacctggccg agttccactt gaagctgata
     2221 gctgcagcag cggccgggct ggccgatccg atcagcatcg tcgacctgca gcgccgcgaa
     2281 ctgctgcggc ggttgcgcga cgtccagcag gcagcgatgg cagagcccaa tcagtcggac
     2341 gccgggttgg tgctcgaagg catcgtcctg cggcttcagg cagacctgcg ctggctcgag
     2401 tcgtgtgaac gcgtctggag tggtcgaggg agcaagcgat gaacgagagc gtgctgttgc
     2461 gcgcccgcgg gttgcggaag ctttacggca gcggtgaggg acttgtgcgt gccgtcgacg
     2521 atgtcgacct cgacgttatt cgtggcgaga tgctcgcggt gatggggccg agtggctgcg
     2581 ggaagtcaac gcttctacat ctcctcggcg ggttagacag gcccacggcc ggcgagctct
     2641 cactggacgg gcggcgaatc gatcaactct cggagcgcgc cctggcacag cttcgccggc
     2701 acgaggtcgg cttcgtcttc caggcgtacc acctcatgga tgagctgacc gcccaggaaa
     2761 acatcgaatt gccagcactt ctcgcaggcc gcgcaccgcg agacgctcag cgtcgtgctc
     2821 ggcagctgct tgagcaagta gggctcgctg atcgggccga tcacctcccg tcagcgttgt
     2881 cgggtggtca gcgtcaacgg gtagcgatcg ctcgggcgct cgccaacgag ccgcttgttg
     2941 ttctcgccga cgagcccacc ggcaacctcg acagtgcggc aactcttgag gtgctacggc
     3001 ttttcgaagt gctgcacaac gacggactca ctctcgtcat cgtcacgcac gacgagcgga
     3061 tcgctgcgac tgccgatcgg ctgatctcga tgcgcgacgg tgcccttgtc gacgagacgc
     3121 gcctcgtcga cagcgccagg agaaatctcg ccgacctcgc cgggctggag acgtaggtga
     3181 aggggcgcgt gttccttgtc tttcgcctcg cggttaagga cctccgccac cgaccagcac
     3241 aggcactgct cctgctactc gcaatcgctg ccggcacgac gacactcacg ctcggccttg
     3301 cacttcacgg cacgacgaac aatccgtatg cacgaacgcg agccgcaacc aacggaccgg
     3361 atgtggtggc gaccgtcatg cccggcggct caagtgcccc agcgccggca attaccgcca
     3421 cgccgggagg ggctgttgaa c
//
LOCUS       DATCBJ010000313         7106 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_112622, whole genome shotgun sequence.
ACCESSION   DATCBJ010000313 DATCBJ010000000
VERSION     DATCBJ010000313.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 7106)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7106)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7106
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_112622"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..391)
                     /locus_tag="VII50_06260"
     CDS             complement(<1..391)
                     /locus_tag="VII50_06260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VIT1/CCC1 transporter family protein"
                     /protein_id="HEY5050485.1"
                     /translation="MTAGPDQVAAAQPRPEVHHDHRDVTGGWLRPAVFGAMDGLISNF
                     SLISGFAGANASRHLVILSGLGGLVAGAFSMAVGEYVSVASQGDLARAEIEVERYELR
                     HNAAAEQAELAELYISRGVDRALALEVS"
     gene            complement(388..1761)
                     /gene="lgt"
                     /locus_tag="VII50_06265"
     CDS             complement(388..1761)
                     /gene="lgt"
                     /locus_tag="VII50_06265"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: protein motif:HMM:TIGR00544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HEY5050486.1"
                     /translation="MTFQSLHDYIPSPSDGVWHLGPVPVRGYALCIIVGIFAAAVLAD
                     RRYRARGGALGVINEISTWAVPFGLVGGRLYHVLTDWSDYFGKGGSPIRSLEVWKGGL
                     GIPGAVALGGLGVLIGCRRRGVLMPPIADAIAPGLVLAQGIGRWGNWFNQELYGRPTS
                     LPWSVRIDAAHQCGVITKAYETAAVQAQCQSGIFSNVYGYFQPTFLYESIWDVGTAIA
                     LIVLDRKYKLGHGRVFALYLVIYGVGRSWIEALRIDPAHHFYGLRLNDWTSIAIVLAG
                     VIGYQVSARRHPGREVVLVRGVSIETETALDSATEPASQTAESDEVVAPDVPDLAEPA
                     DVAEPPEPRELLQPRELFEPRELVEETEPTAAPDPVDGPEAAESGDEAESEPLEPVEH
                     DAAEPVESVDEVEPGAEAETEPVESVDEAESEPAEHDEAEPEPAELADENLVVEADTP
                     STHGGES"
     gene            complement(1787..2611)
                     /gene="trpA"
                     /locus_tag="VII50_06270"
     CDS             complement(1787..2611)
                     /gene="trpA"
                     /locus_tag="VII50_06270"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HEY5050487.1"
                     /translation="MTAMPATFAPTKLHETLARCTAEGRAALIGYLPACFPTPDRGIA
                     VLNALVEGGVDAIEIGLPYSDPVLEGPTIARSHDIALRAGSTTAHVLRTVQAVAATGV
                     PTVVMTYWNPVERYGVERFAADLAAAGGCGLITPDLLPDEAEPWVEASDAHGLDRIFV
                     VAPSSTDRRLAMTARFCRGFIYAASVMGVTGTRAQTSSLAPGLVDRIRATTSLPVGVG
                     LGVSNAEQAAEVAGYADAVIVGSAFVRRILEARGTQDEAVAVRTLAAELAAGVRRR"
     gene            complement(2608..3825)
                     /gene="trpB"
                     /locus_tag="VII50_06275"
     CDS             complement(2608..3825)
                     /gene="trpB"
                     /locus_tag="VII50_06275"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HEY5050488.1"
                     /translation="MIAPKLPDASGHFGPFGGRFVPEALVAALDELGVAYTDAMADPA
                     FTAELARLLSTYAGRATPITDATRLSEHAGGARILLKREDLTHTGSHKINNVLGQALL
                     TVRMGKKRVIAETGAGQHGVATATAAALFGLECTIYMGEEDTRRQALNVARMKLLGAE
                     VVPVTAGTRTLKDAINEAFRDWVTTVEETHYLFGTVAGPHPFPALVRDFQKVVGEEAR
                     AQVLALTGRLPDAVTACVGGGSNAIGIFTAFLPDNSVRLYGFEAGGDGVATGRHAATL
                     SAGTRGVLHGARTYVLQDSDGQTMASHSISAGLDYPGVGPEHAWLAETGRVTYLPITD
                     SEAMDAFLLLARTEGIIPAIESAHALAGTLHLGRQLGPDAVVLVSLSGRGDKDLDTAA
                     SWFNLDTSPTGVR"
     gene            complement(3860..4666)
                     /gene="trpC"
                     /locus_tag="VII50_06280"
     CDS             complement(3860..4666)
                     /gene="trpC"
                     /locus_tag="VII50_06280"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004561847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HEY5050489.1"
                     /translation="MSVLDEILVGVRADMEQRQRAMPLDELKLKAAKRTAPADGVSAL
                     KRDAVTVIAEVKRSSPSRGELAAITDPAALACAYEAGGAAVVSVLTEPRKFHGSLADL
                     EAVRKAVDIPLLRKDFIYHSYQLWEALAYGADLVLLIVAALDQSALVSLVERAQSLGL
                     TPLVEVHDEVEVDRAVEAGARVIGVNARDLHTLEIDRTLFARLAPRIPPGIVKVAESG
                     IRGPHDLFAYASAGADAVLVGEGLVTDKDPRQAVADLVTAGAHPALRHGQ"
     gene            complement(4792..6204)
                     /locus_tag="VII50_06285"
     CDS             complement(4792..6204)
                     /locus_tag="VII50_06285"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEY5050490.1"
                     /translation="MPKRRTGLATRIALVTTLAAAIAVLIAGIVLARVISKAADDTGR
                     KVLMQYASLSVDAADVSRTGRVAVRAQIIALLRTQQVQLVVVQPDGLVTGPGGAVATT
                     GIVTPTEVAELKAGTGFSELRRIGGGRYYLEGRPITTGSAAGGGVVLVQRVADARSAG
                     SQVGLRLLVALIAGLVVAALTGVLLARRLARPLQHAAGAARRLSTGARDVRLVPEGPA
                     EVAAVADALNALSGALTTSEGRQREFLLSISHELRTPLTAVKGYAEALADGVVPPAAV
                     EATGQVLLAESSRLERLVSDLLDLARLGAEDFRIDVTRLDLVALVRQAATVWSDRCLK
                     EGVEFGLEAPGWPVLVDTDPTRVRQIIDGLAENALRVTPAGRPIVFFVGASGSEAILQ
                     VRDGGPGLTEDDCKVAFDRSALYDRYRGVRRVGTGVGLALVAGLTARLGGRAAAGRSP
                     EGGACFTITLPLRPGGAKAD"
     gene            complement(6197..6916)
                     /locus_tag="VII50_06290"
     CDS             complement(6197..6916)
                     /locus_tag="VII50_06290"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEY5050491.1"
                     /translation="MTEPSVPGGPAGDGSAKGLVLVIEDERAIADLERMYLTREGFGV
                     HVESDGRAGLAAARNLHPVAIVLDVGIPTIDGTEVCRILRADGDWTPILFVTARDEEI
                     DRILGLELGADDYLTKPFSPRELVARVKAVLRRSSTDAGSQERVYVVGRVTVDPARRR
                     VNADGQPIDLTATEFELLEFLCRRPGRVFPREHLLSEVWGYAAAAGTRTVDVHVAQLR
                     AKLGDASPIRTVRGVGYSADA"
BASE COUNT         1208 a         2485 c         2333 g         1080 t
ORIGIN      
        1 tggaaacttc gagcgcaagc gcgcggtcga cgccacggga aatgtaaagc tcggcgagtt
       61 cggcctgctc ggcggcggca ttgtgtcgca gctcgtaccg ctcgacctcg atctcagccc
      121 gtgccaggtc accttgagaa gccaccgaca cgtactcacc gacggccatc gagaacgcgc
      181 ccgcgaccaa gccgcccagt ccgctcaata tcaccaggtg tcgcgacgcg ttggcaccgg
      241 cgaagccgga gataagcgag aagttcgaga tcaggccgtc catcgcgcca aagaccgccg
      301 gccgcagcca gccgccggtg acgtcacggt ggtcgtgatg gacttccggg cgcggctggg
      361 ccgcggcgac ctggtcgggt ccggcggtca cgactcacct ccgtgggtcg acggcgtgtc
      421 cgcttccacc accaggttct catccgccag ctcggcgggc tcgggctcgg cctcgtcatg
      481 ctcggcaggc tcggactcag cctcgtcgac agactcgacc ggctcggtct ccgcctccgc
      541 gccaggctcg acctcatcga cagactcgac cggttcggcc gcgtcatgct caacaggctc
      601 gagaggctcg gactctgcct cgtcgccaga ctcagcagcc tcgggcccgt ctaccggatc
      661 gggtgccgcc gtcggctcgg tctcttccac gagctcgcgt ggttcaaaaa gctcgcgtgg
      721 ttgaagaagc tcgcgtggtt ctggcggctc agcgacatcg gcgggttccg cgagatccgg
      781 gacatccggc gcgaccacct cgtccgattc ggctgtctga cttgccggct cggtcgccga
      841 atcgagggcg gtctcggtct cgatcgaaac gccgcggacc agcaccacct cgcggccggg
      901 atggcgtcgg gccgatacct ggtatccgat gacgccggcc aaaacgatcg cgatgctcgt
      961 ccagtcgttg agtcgcagac cgtagaagtg gtgcgcgggg tcgatccgca gcgcctctat
     1021 ccaactgcgc ccgacgccat agatgacgag gtagagcgcg aatacgcgac catggcccag
     1081 cttgtacttc cggtcgagca cgatcagggc gatggcagtg cccacatccc agatcgactc
     1141 gtagaggaac gttggctgaa agtagccgta cacgttcgag aaaatccccg actggcattg
     1201 cgcctgcacg gccgcggtct cgtaggcctt cgtgatgacg ccgcactggt gcgctgcgtc
     1261 gattcgcacc gaccagggca atgaggtcgg cctgccgtag agctcctggt tgaaccagtt
     1321 cccccagcgg ccaatccctt gagccaacac gagacccggc gcgatggcgt cggcgatcgg
     1381 cggcatcaat acgccgcgac gacggcagcc aattaataca ccgaggccgc caagggcgac
     1441 tgctccggga atgccgaggc cgcctttcca gacctccagt gagcgaatcg gacttccgcc
     1501 cttgccgaag tagtccgacc agtctgtgag gacgtggtaa agccggccac cgacgagtcc
     1561 aaacggcacc gcccaggtcg agatctcgtt gatcacgccc aacgcgccgc cacgggcgcg
     1621 gtagcgccga tcggccagca ccgcggccgc gaagatgccg acgatgatgc acaacgcgta
     1681 gccacgaacg ggtaccggcc cgaggtgcca cacgccgtcg ctcgggctcg ggatgtagtc
     1741 gtgcagcgac tgaaaggtca aggcaggcgt catcactgtt acgacgctat cggcgccgca
     1801 cgccggccgc gagttcggca gccagcgtgc gtaccgcgac cgcctcgtcc tgcgtgccgc
     1861 gcgcttcgag gatccgacgc acgaatgccg agccgacgat gaccgcgtcg gcgtagccgg
     1921 cgacctcggc cgcctgctcc gcgttgctga cgccgagtcc gacgcctacc ggcagcgacg
     1981 tcgtggcccg gatccggtcg accaagccag gggccaaaga actcgtttgc gcgcgggtgc
     2041 cggtcactcc catcaccgat gcggcgtaga tgaagccgcg acaaaatcga gccgtcatgg
     2101 ccaaccgccg gtcggtgctg ctcggcgcca ccacgaaaat gcggtcgaga ccgtgggcgt
     2161 cgctcgcctc gacccaaggt tccgcctcgt caggcaacag gtcgggggtg atcagcccgc
     2221 agccgccagc cgccgcgagg tcggcagcga atcgctcgac gccataccgc tcgaccggat
     2281 tccagtaggt catcaccacg gtcggcaccc cggtcgcggc aaccgcctgc acggtgcgca
     2341 gcacgtgcgc ggtcgtcgag ccagctcgca gtgcgatgtc gtgcgagcgc gcaatagtcg
     2401 gaccttcgag caccggatcg ctgtacggca gaccgatctc gatcgcgtcg acgccaccct
     2461 cgaccaatgc gttcagtacc gcgatcccgc ggtccggggt agggaagcac gccggcaggt
     2521 agccgataag cgcggcgcgg ccttctgccg tgcagcgggc gagggtctcg tgcagtttgg
     2581 tcggcgcgaa cgtcgcaggc atcgcggtca tcgaaccccc gtcggcgagg tgtcgaggtt
     2641 gaaccacgac gccgcagtgt cgaggtcctt gtccccgcgc ccggacagac tcaccagtac
     2701 gacggcgtcc ggtccgagtt gacggcccag atgcaaggtg cccgcgaggg catgcgcgct
     2761 ttcgatcgct gggatgatgc cctcggtcct agcaagcagc aagaacgcgt ccatcgcttc
     2821 ggaatcggta atcggcaggt acgtgacccg gcccgtctcg gccagccacg cgtgctcagg
     2881 acccacgccc ggataatcga ggccggcaga gatcgaatgt gacgccatcg tctggccatc
     2941 ggagtcctgc aagacgtagg tccgagcgcc gtgcagaacc ccacgtgtgc cggcgctcaa
     3001 cgtcgccgcg tggcggccag tcgcgactcc gtcgccccct gcctcgaatc catacaggcg
     3061 caccgagttg tcgggcagga atgcggtgaa gattccgatc gcgttggagc cgccaccgac
     3121 acaggcagtg acggcgtcgg gcagccggcc ggtgagcgcg agcacctgag cacgggcttc
     3181 ctcgccgacc accttctgaa aatcgcggac cagcgcgggg aacgggtgcg gaccagcgac
     3241 ggtgccaaaa aggtagtgcg tctcctccac cgtggtgacc cagtcgcgga aggcctcgtt
     3301 gatggcgtcc ttcagcgttc gggtgccggc ggtcaccggg acgacctcgg cacctagcag
     3361 tttcattcga gcgacgttga gggcctggcg acgggtgtcc tcctcgccca tgtagatcgt
     3421 gcactcgagc ccgaagagcg cggccgctgt tgccgtggca accccgtgct gcccggcgcc
     3481 ggtctcggcg atcacccgct tcttgcccat ccgcaccgtg agcagcgcct gacccaagac
     3541 gttgttgatc ttgtgtgaac cggtgtgagt gaggtcctcg cgcttcagca ggatccgggc
     3601 gccgccggcg tgctcggaga gtcgggttgc atcggtgatc ggcgtcgcgc ggccggcgta
     3661 ggtcgacagc agtcgagcga gttctgccgt gaacgccggg tcggccatgg cgtccgtgta
     3721 ggcgacgccc aactcgtcga gcgcggcgac gagagcttca gggacgaacc ggccgccgaa
     3781 ggggccgaaa tggccacttg cgtcggggag cttgggcgcg atcaccgatc aagcctaggg
     3841 gccgcaaacc gcgccaccat tactgaccgt gccgcaaggc cggatgcgcc cccgcggtga
     3901 caaggtcggc gaccgcctga cggggatcct tgtcggtcac cagcccttca cccacgagca
     3961 ctgcatcggc gcccgccgac gcatacgcga acaggtcatg cggcccgcgg atacccgact
     4021 ctgcaacctt cacgatgcca gggggtatac gcggagcgag ccgcgcaaac agcgtgcggt
     4081 cgatctcgag ggtgtgcagg tcgcgcgcgt tcacgccgat gacccgggcg cccgcctcga
     4141 cggcccgatc gacctcgacc tcgtcgtgca cttcgacaag tggggtcagg ccaagcgatt
     4201 gggctcgctc gaccaacgac accaacgcgc tctggtcaag tgcggcaacg atgaggagca
     4261 ccaggtcggc gccgtaggcg agggcttccc acaattgata cgagtggtag atgaagtctt
     4321 tacgtagcaa gggaatgtcg acggctttgc gcacggcctc gaggtcagcg agcgacccat
     4381 ggaacttgcg cggttcggtc agcacactga cgactgcggc accgccggcc tcgtacgcgc
     4441 acgcgagcgc agcggggtcg gtgatggccg cgagctcgcc gcgggaggga ctcgaccgct
     4501 tcacttctgc gatcacggtc accgcatcgc gcttgagtgc ggacactccg tcggccggag
     4561 cggtgcgctt cgcggccttg agcttgagtt catcgagcgg catcgcgcgc tggcgctgct
     4621 ccatatcggc gcggacgccg accaggatct cgtcgagcac gctcacctag cgatcccctc
     4681 gtgcaccctg ttagctggct gtcgagcact gtctggaact gcctcagggt agcggccagg
     4741 gaaatcaacc cggccgtcag cccccagggc agcagcccct cagcctccgg atcaatcggc
     4801 tttcgcccct ccaggccgca gcggcaaggt gatggtgaag caagccccac cctcgggcga
     4861 cctgcccgcg gcggcgcggc cgcccagccg cgccgtgagg ccggccacta gcgcgagccc
     4921 gacgcccgtt ccgaccctgc ggacgccgcg atatcggtcg tacaacgcgg agcgatcgaa
     4981 cgcgaccttg cagtcgtcct cggtaaggcc cggcccgcca tcgcgcacct gcaggatcgc
     5041 ctcggacccg gacgcgccga cgaagaacac gatgggccgc cccgccgggg tcacgcgcag
     5101 cgcgttttcg gccagaccgt cgatgatctg ccgcacccgc gtcggatcgg tgtcgaccag
     5161 gaccggccaa cccggcgcct caagaccgaa ctcgacgccc tccttcaggc agcgatctga
     5221 ccagacggtc gctgcttgcc gtacaagcgc gacgaggtcg agcctggtga cgtcgatccg
     5281 gaaatcctcg gcgcccagtc tggcgaggtc gaggaggtcg gagaccagtc gctcaaggcg
     5341 gctcgactcc gccagcagca cctgaccggt cgcctcgaca gcggcgggcg gcaccacgcc
     5401 gtccgccaat gcttcggcat atcccttgac cgcagtcagc ggcgtacgca gctcatggga
     5461 gatcgacaac aggaactcgc gttgccgacc ttcgctcgtc gtgagagcac cgctcaatgc
     5521 gttcagcgca tcagccaccg cggcaacctc cgccgggcct tcgggcacca gccggacgtc
     5581 gcgcgctccc gtcgatagtc gtcttgcggc gcctgcggcg tgctgcaacg gcctggccag
     5641 tcgtctcgcc agcaggacgc ccgtgagcgc cgctacgacg agaccggcaa tgagtgcgac
     5701 gagcagccgc agccccacct ggctccccgc ggagcgcgca tcggcaaccc tctgcaccaa
     5761 cacgacgcca ccaccggcgg ccgacccggt ggtgatcggc cggccctcga ggtaatagcg
     5821 accgccgccg atgcggcgaa gctccgaaaa gcccgtgccg gctttgagct cagccacttc
     5881 ggtcggagtg acgatccccg tcgtcgcaac ggctccgccg ggaccggtca cgaggccgtc
     5941 gggctgcacg acgaccagct gcacctgctg ggtgcgcagc aacgcgatga tctgcgcccg
     6001 caccgcgacg cggccagtcc tcgagacgtc cgcggcgtcg acggaaaggc tggcgtactg
     6061 catcagaacc ttgcggccgg tgtcatccgc cgccttactg atcacgcgcg ccaacacgat
     6121 gccggcgatc aggacggcga tcgccgcggc caaggtggtg acgagcgcga tgcgggtcgc
     6181 caggccagta cggcgtttag gcatcggcgc tgtatcccac cccacgcacg gtgcggatcg
     6241 ggcttgcgtc accgagcttt gcgcgcagct gagcaacgtg aacgtccacc gtgcgggtgc
     6301 cggcggccgc ggcgtagccc cagacctccg acagcaaatg ctcgcgcggg aaaacacggc
     6361 cgggccgccg acataggaac tccagcagct cgaactccgt cgccgtgagg tcgatgggtt
     6421 gcccgtccgc gttgactcgg cggcgcgcgg ggtcgacggt caccctgccc acgacgtaca
     6481 cccgctcctg cgagccggca tcggtgctac tgcggcgcag tactgccttc actcgcgcga
     6541 ccagctcgcg cgggctgaac ggcttggtca ggtagtcgtc ggcgcccagt tcgagaccga
     6601 ggatccggtc aatctcttcg tcgcgggcgg tgacgaacag gatcggcgtc cagtcgccgt
     6661 ctgcgcgcaa gatgcggcag acctccgtgc cgtcgatcgt cgggatgcca acatcgagca
     6721 cgatcgcgac cgggtgcaga tttcgcgcag ccgcaagtcc cgccctgcca tccgattcga
     6781 catgaacccc gaacccctca cgggtcaggt acatccgctc gaggtcggcg atagcccgct
     6841 cgtcctcgat gaccaggacc agacccttcg cggaaccgtc gccggccggc ccgccgggca
     6901 cgctaggttc cgtcatggtg agcctcctgg tacggacgct agaacagcat cgccgtccac
     6961 atcgtcggca acgtctgcgc aacgccgtgt tcgagaattg ttcgagccat gtatgaggcc
     7021 tcaggcggtg gggtcgtcgc cctcgtcgag gcggtcccac atcgacgttt cggtggccgg
     7081 cccgcgcttg gccgaacccg ccgcct
//
LOCUS       DATCBJ010000314         2760 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_427919, whole genome shotgun sequence.
ACCESSION   DATCBJ010000314 DATCBJ010000000
VERSION     DATCBJ010000314.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2760)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2760)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2760
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_427919"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..405
                     /locus_tag="VII50_06295"
     CDS             <1..405
                     /locus_tag="VII50_06295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEY5050492.1"
                     /translation="PAALDAGLSLLAWAPLGRGVLTGKYRTGIPADSRAASDHLASFV
                     EPYLVGTGSGIVQSVCTAAEGLGVSPLAVALGWVRDRPGVAAAIVGARTNAQLLGILA
                     EADVVLPDEIRGALDDVSMPAIGYPEAGAGQD"
     gene            complement(416..715)
                     /locus_tag="VII50_06300"
     CDS             complement(416..715)
                     /locus_tag="VII50_06300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5050493.1"
                     /translation="MPDEIPATPEQVHAAVAGLKAALDAHLAAVEARAGENDPHVQAA
                     YDALASAGETYDDALFSAYEEVTPFGPVESDVDEADDEADAEEDDDLEWEAPEAE"
     gene            838..1023
                     /locus_tag="VII50_06305"
     CDS             838..1023
                     /locus_tag="VII50_06305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006135970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5703 family protein"
                     /protein_id="HEY5050494.1"
                     /translation="MADYEFRQMVLPRGTSRNAAWRLLTEQAEHHGWELDRLRLYPDG
                     TRRVRLRRRVLRVARTM"
     gene            complement(1035..2342)
                     /locus_tag="VII50_06310"
     CDS             complement(1035..2342)
                     /locus_tag="VII50_06310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009078702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEY5050495.1"
                     /translation="MSQPQAAPSAEDEVVDLCRDLIRIDTSNPIKPERPAAEYVAEKL
                     TDVGLTPQLFESEPGRASVVARIEGSDSSRGALLIHGHLDVVAADPAHWQVDPFAGDI
                     KDGCLWGRGAVDMKDMDAMSLAVIRQWMREGRKPPRDIVIAYLADEEAGGRKGSHWLV
                     DNQPELFEGCTESISEVGGFSLEIRPDLRLYPVQTAQRGMAWMKLVARGRAGHGSLVN
                     NANAVTELAEAVSRIGRYDWPVRMTAATQAFLDELGDAIGIELDPHDLSSVADKLGTI
                     TALVAATVRNTANPTMLEAGYKVNVVPQEASAHIDGRFLPGFEDEFYATIDELLGPNV
                     TRETVNEDIAIETEWDGALVEAMKSSITSQDPQGKAVPYCLFGGTDDKAFSQLGIRGF
                     GFSPLRLTPDLDFAGMFHGVDERVPLEGLKFGTRVLDNFLTNC"
     gene            2470..2554
                     /locus_tag="VII50_06315"
     tRNA            2470..2554
                     /locus_tag="VII50_06315"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2505..2507,aa:Leu,seq:gag)
BASE COUNT          422 a          916 c          917 g          505 t
ORIGIN      
        1 cccgctgcgc tggacgccgg cctgtcgctg ttggcgtggg caccactcgg gcggggtgtg
       61 ctgaccggca aataccggac cgggatcccc gccgattcgc gcgccgcgag tgaccacttg
      121 gccagcttcg tcgagcccta ccttgtgggg accggctccg gcatcgtcca gtcggtgtgc
      181 acggccgccg aaggcctcgg cgtctcgccc ttggctgtgg cactgggatg ggtgcgcgac
      241 cgaccaggtg tggcagccgc gatcgtgggg gcgcgcacga atgcgcaact gctcggcatc
      301 ctggccgaag ccgacgtcgt cttgccggac gagattcgtg gcgcactcga cgatgtgtcg
      361 atgccggcga tcggataccc agaagccggg gcggggcagg actagcgctg ttccgctact
      421 cggcttctgg tgcttcccac tcgagatcgt cgtcctcttc ggcgtccgct tcgtcgtcgg
      481 cctcatcgac gtcggactcc accggaccga acggcgtgac ctcttcgtag gccgagaaca
      541 aggcatcgtc gtaggtctcg ccggccgagg cgagcgcgtc gtaggccgcc tggacgtgcg
      601 gatcgttctc ccctgcccgc gcctcgaccg cggcaaggtg ggcgtcgagg gcggccttca
      661 gcccggcgac ggccgcgtgg acctgctctg gtgtggcggg gatctcgtcg ggcatggggt
      721 gaacggtacc tgcctccccc tgggtgtgcg caacggcgat ctttcgaggc caccgaatcg
      781 atgaggaact gataccgacc tgctgccgag aaggtaccgc ggcgcgcaca atgaaccgtg
      841 gccgactacg agttccggca gatggtgctg ccgcgcggaa cgtcgcgcaa cgctgcctgg
      901 cgtctactta ctgagcaggc cgagcatcac gggtgggagc tcgaccggtt gcgactctac
      961 ccggacggaa cgcggcgggt gcgcctgcgc cgccgggtct tgcgagtcgc tcggaccatg
     1021 tagccggggg cggttcagca gttcgtcaag aagttgtcga gcacgcgggt gccgaacttc
     1081 aagccttcga gcggaacccg ttcgtcgacg ccgtggaaca tgccggcgaa gtcgaggtcc
     1141 ggcgtgagcc gcagcgggga gaacccgaac ccgcgtatcc ccaactgtga gaacgccttg
     1201 tcgtcggtgc cgccgaataa gcagtacggg acggctttgc cctgcggatc ctgagacgta
     1261 atggatgatt tcatcgcctc gaccaacgcg ccgtcccact cggtttctat cgcgatgtcc
     1321 tcgttgacgg tctcgcgtgt gacatttggt ccgagcaatt cgtcgatcgt cgcgtagaac
     1381 tcgtcttcga agcccggcag gaaacggccg tcgatatgcg ccgacgcctc ctgcggcacg
     1441 acgttcacct tgtagccggc ctcgagcatc gtcgggttcg cggtattgcg gaccgtggcc
     1501 gccaccagcg ccgtgatcgt tccgagcttg tcggcgacac tgctcaggtc gtgggggtcg
     1561 agctcgatgc cgatcgcatc gcccagctcg tcgaggaatg cctgggtggc cgccgtcatg
     1621 cgcaccggcc agtcgtacct gccgatgcgc gagaccgcct ccgcgagctc ggtcacagca
     1681 ttggcgttat tgaccaggct gccgtggccg gcgcggccgc gagcgaccag cttcatccag
     1741 gccatcccgc gttgcgctgt ctgcaccgga tacagacgca ggtcggggcg aatctcgaga
     1801 gagaaaccgc cgacctcgct gatggactcg gtgcaacctt cgaacagctc gggttggttg
     1861 tcgaccagcc agtgcgagcc cttgcggccg ccagcctctt cgtcggcgag gtaggcaatc
     1921 acgatgtcgc gcggtggctt gcggccttcc ctcatccact gccggatgac cgcgagcgac
     1981 atcgcgtcca tgtccttcat gtcgaccgcg ccgcgacccc acaagcagcc gtccttgatg
     2041 tcgcccgcga atggatcgac ctgccagtgg gccgggtcgg cggccaccac atcgaggtgt
     2101 ccgtggatca gcagggcgcc gcgactggag tcgctgcctt caatacgcgc gacgacgctg
     2161 gcccggccgg gttcggactc gaagagctgc ggcgtcagac cgacgtccgt cagcttctcc
     2221 gcgacgtact cagccgccgg ccgctcgggc ttgatcgggt tggaggtgtc gatacggatc
     2281 aagtcgcggc agaggtcgac gacctcgtcc tccgcgctcg gggccgcctg cggttggctc
     2341 atcagctctc ctcgcgcgaa cgcacagtca tatgccggac gactgccatc ctgcccggtg
     2401 gcgtgaccgg cgtctgcctc ggcctcagcg ttgatatggt gtttgccggt cctgcgccgc
     2461 aggactcacg tccgggtggc ggaatttggc agacgcgcta gcttgaggtg ctagtgcccg
     2521 aaagggcttg ggggttcaag tcccccctcg gacacactat ttgcgcaggt cacaggccta
     2581 cgccgctgca tttggtgccc ccatggttgc acaccccaaa cgtccaaacg gacagatgcg
     2641 ttccctagca ggcacatcaa catgttggtc cccgcctggt ccccctctgg tcgctgcacg
     2701 ttggcgtcgc gccatctcca atcgctgaca cgatcgtgag gatgacgcgt tcatcaacag
//
LOCUS       DATCBJ010000315         2057 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_119224, whole genome shotgun sequence.
ACCESSION   DATCBJ010000315 DATCBJ010000000
VERSION     DATCBJ010000315.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2057)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2057)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2057
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_119224"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..221)
                     /locus_tag="VII50_06320"
     CDS             complement(<1..221)
                     /locus_tag="VII50_06320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5050496.1"
                     /translation="MNTASLEPANRSTVTRRIDFRPGMDMWWEIVRNGEDTDGEVLEV
                     NSWLGPHQPSPPVHVHDNAEDSFEIVEGRL"
     gene            375..878
                     /locus_tag="VII50_06325"
     CDS             375..878
                     /locus_tag="VII50_06325"
                     /inference="COORDINATES: protein motif:HMM:NF024854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEY5050497.1"
                     /translation="MPKSIQILLRDARSAIEAAVRADLAQNGFGDVAPSHSALLRNVG
                     EDGSRPSDLAAHAGVTRQAITKLVDELERLDLVRRDPDPDDGRGVIVRYTDRGRAGVA
                     IARKRMLALERAYAAQVGADRWAEVRSTLETLFGDDPPQDLSPPTAPDLSNRERLDHN
                     QARATGE"
     gene            961..1233
                     /locus_tag="VII50_06330"
     CDS             961..1233
                     /locus_tag="VII50_06330"
                     /inference="COORDINATES: protein motif:HMM:NF013677.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEY5050498.1"
                     /translation="MPGPRKYPEELVARGVRLAIESGRPVAHVAKDLGLPSETLRKRV
                     RQAEADGGLRPDLPSSEEREEIKALRKEVFELRRANEMLKAASVFF"
     gene            1267..>2057
                     /locus_tag="VII50_06335"
     CDS             1267..>2057
                     /locus_tag="VII50_06335"
                     /inference="COORDINATES: protein motif:HMM:NF033516.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HEY5050499.1"
                     /translation="MFIDEHRDRFGVEPICRTLGVSASAYYHRRHGELSAREVKDRRL
                     TERIREIHQANYEAYGYRRTWKALRRAGEQAPRCQVQRLMRAHGICGAKRRGKPWRTT
                     IAVPATQRRSDLVGRDFTAPAPDRLWVGDLTYLRCWEGVVYLAFVIDVFSRKVVGWQL
                     AGHMRTSLVLDALAMAVGTRQPGADFQLVAHSDRGSQYTSGLYTQTLDDHHILGSVGS
                     VGDAYDNAVAESFVDSVKTELIADRVLQTRTQLELAIIEYLGWFNG"
BASE COUNT          356 a          683 c          659 g          359 t
ORIGIN      
        1 agtcggccct cgacgatctc aaagctgtct tcagcattgt cgtgcacatg cacagggggg
       61 ctgggttggt gaggaccgag ccagctattt acctcaagaa cttcaccatc ggtgtcctcg
      121 ccgttgcgca cgatctccca ccacatatcc atgcccgggc gaaagtcgat gcgccgagtc
      181 acggtagagc ggtttgcagg ttcaagggaa gcggtattca caggggctcc attcggtcgt
      241 tggttctcta ttggttgcga ctcaggcgac agcggggccg ccgctgctgg ttgttcacct
      301 tggttgacaa gttgagagaa ccttctcacg gcgcgtcgtg tttgtcaagc gaggtgaact
      361 acaatcgtgt ggacatgccg aagagcatcc agatccttct ccgcgacgcc cgcagcgcga
      421 tcgaggccgc cgtccgcgcc gatctcgcac agaacggctt tggcgatgtc gcgccctcgc
      481 actccgcgct cttgcgcaac gtcggcgagg acggttctcg ccccagcgac ctcgccgcac
      541 acgccggcgt cacgcgccaa gccatcacca agctcgtaga cgagctcgag cggctcgatc
      601 tcgtgcgccg cgacccagac ccggacgacg ggcgcggcgt gatcgtcaga tacaccgacc
      661 gcggacgcgc cggcgttgct atcgctcgca agcggatgct cgcgctcgag cgcgcctacg
      721 ccgcgcaggt cggcgccgac cgctgggccg aggtgcgctc aaccctcgaa accctattcg
      781 gtgacgatcc tccccaggac ctgagcccgc ctaccgcccc agacctatcc aatcgggaac
      841 gcctggacca caatcaagca cgcgcgaccg gtgagtgagc ggtctgagcc aggccaggct
      901 gtttcgcgga gacttcgaat cgaggacgat ctgtccttgg ggattggaga ccctgctgct
      961 atgccaggac cccggaagta ccccgaggag ctggtcgcgc gcggtgtgcg gctcgcgatc
     1021 gagtctggcc ggccggtcgc tcacgtcgcg aaagatctag ggctgccgtc tgagacgttg
     1081 cgcaagcgtg tccgtcaggc tgaggccgat ggtggtctga ggccggatct gcccagcagc
     1141 gaggagcgtg aggagatcaa ggcgttgcgc aaagaggtgt tcgagttgcg tcgcgccaac
     1201 gagatgctga aggccgccag cgtgtttttc tgagaccgag ctcgacgcag accgaacgaa
     1261 gtgagcgtgt tcatcgatga gcatcgcgat cgcttcggcg tcgagccgat ctgccggacc
     1321 ctgggcgtgt cggcgtccgc ttactaccat cgccggcacg gcgagctgtc agctcgcgag
     1381 gtgaaggatc gacggctgac cgagcgcatc cgtgagatcc accaggccaa ctatgaggcc
     1441 tacgggtatc gccgcacctg gaaggcgctc cgccgcgctg gggaacaggc cccgcgctgt
     1501 caggtgcagc gcctgatgcg cgctcacggc atctgcggcg cgaagcgtcg cgggaaacca
     1561 tggcgcacca cgatcgccgt cccggccacg cagcgccggt ctgatctggt cgggcgcgat
     1621 ttcaccgcgc ccgcaccgga tcgcctctgg gtgggggacc taacctatct gcgctgctgg
     1681 gaaggcgtcg tctacctggc gttcgtcatc gacgtgttca gccgcaaggt cgtgggctgg
     1741 cagctggccg gtcacatgcg caccagcctc gtgctcgacg cgctggcgat ggcggtcggc
     1801 acccgccaac ccggcgctga cttccaactc gtcgcccatt ctgatcgcgg cagtcaatac
     1861 acctccgggc tctacaccca gaccctggac gaccaccaca tcctggggtc ggtcgggtca
     1921 gtcggcgacg cctatgacaa cgccgtcgcc gagtccttcg tggactcggt caagaccgag
     1981 ctgatcgccg accgcgtctt gcagacccgc acacagctcg agctcgccat catcgaatac
     2041 ctcggctggt tcaacgg
//
LOCUS       DATCBJ010000316         2323 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_269196, whole genome shotgun sequence.
ACCESSION   DATCBJ010000316 DATCBJ010000000
VERSION     DATCBJ010000316.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2323)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2323)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2323
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_269196"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2138)
                     /locus_tag="VII50_06340"
     CDS             complement(<1..2138)
                     /locus_tag="VII50_06340"
                     /inference="COORDINATES: protein motif:HMM:NF015644.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0182 family protein"
                     /protein_id="HEY5050500.1"
                     /translation="MTFPRPERRPYGAGNGGPRQPLPRPQFPSWGKPLLITLGIVVVL
                     VVAIALFVAIDTEHLWYDSVGFGEVFTTKLVTRLALFAGFGVFMAAMTFGNVYLAYRL
                     RPAFRASSVEQIILDRYRTVIEPRRWLVFGVIAAFFGLIFGTAASGRWQTWLLWSNAT
                     SFGHKDPQFHRDVSYFAFILPFQRFILGYVMALIVVCTLLTLASHYVFGGVAFRTPGE
                     RVTRGARTHLALLLFVFVICKAIAYYLDRFGLAFSSRGAVQGPSYTDVHAVLPAKEIL
                     LVIALLCAGLFIATIFRTGWLLPAVSFGLLVFSAIIIGGIYPLVVQNFQVKPSEADKE
                     APYIQRNINETRYAYGLNNVKVTPYAATTTVSASALTNSTAIGAGVRLLDNAVLPPTY
                     NQLQQFRNFYNIDKLDVDRYIINGQEQDAVVATRELNLAGVPASQKTWINEHLVYTHG
                     YGFVAAPGDGVDNDGRPSFIEDNIPVTGSLGTFQPRVYFGEGETSFSVVGLPAGASPR
                     EYDFPTNGANGAQYNTYTGGGGVAIGSTFRQLEYALRFGDKNLVLSDGINSDSRILYD
                     RDPRQRVAKVAPYLTLDGDPYSAIVDGHLVWIVDGYTTSDGFPYSQRENLNAITQDTN
                     TATATTTAQSSEKINYMRNSVKATVDAYTGAVTLYAWDPTDPVLKTWMKVFPGTVKPV
                     SAMSAQLLAHVRYPEDIFKVQRNLLAKYHVT"
BASE COUNT          479 a          697 c          737 g          410 t
ORIGIN      
        1 gtgacgtggt acttcgcaag caggttccgc tgcaccttga agatgtcttc gggatagcga
       61 acgtgcgcga gcaactgcgc actcatcgcg gataccggct tcacagtgcc cgggaaaacc
      121 ttcatccacg ttttgagaac aggatcggtc ggatcccacg cgtagagagt gactgccccg
      181 gtataggcgt caacggtcgc cttaaccgag ttccgcatgt agttgatttt ctcgctcgac
      241 tgcgccgtcg tcgtggcggt cgcggtgttc gtgtcctgag ttattgcgtt caggttttcc
      301 ctttgtgaat aagggaatcc gtcgctcgtg gtgtagccat cgacaatcca caccaggtgg
      361 ccgtcgacga tggccgagta gggatcgccg tcgagcgtga ggtacggggc gacctttgcc
      421 acccgctgcc gtgggtcacg gtcataaaga attcgagagt ctgagttgat gccgtcggac
      481 agcacgaggt tcttatcgcc aaagcgcaac gcgtattcga gctgccggaa ggtcgacccg
      541 atcgccacgc cgccaccacc ggtataggtg ttgtactgcg ctccgttggc gccgttcgtc
      601 gggaagtcgt actcccgcgg cgatgctccg gcaggtagac cgacaacgga aaacgacgtc
      661 tctccctcgc cgaaatacac cctcggctgg aacgttccga ggctgccggt gaccgggatg
      721 ttgtcctcga tgaacgaagg tcgcccatca ttgtcaacac cgtcacccgg cgcagccaca
      781 aagccgtaac cgtgcgtgta aacgaggtgc tcgttgatcc atgtcttctg gctcgcgggg
      841 actccggcaa ggttcagctc gcgggtggcg acgaccgcgt cctgctcctg gccgttgatg
      901 atgtaccggt cgacgtccag cttgtcgatg ttgtagaagt ttcggaattg ctgaagctgg
      961 ttgtacgtcg gcggcagtac cgcgttgtcg agcaatcgaa cacctgcgcc gatggcggtg
     1021 ctgttcgtca gtgcgctcgc actcacggtt gtcgtggccg catacggggt gaccttgacg
     1081 ttgttcagac cgtaggcgta gcgcgtctca ttgatgttgc gctgaatgta gggagcttcc
     1141 ttgtcggcct cactcggctt cacctggaag ttctgcacga ccaatggata gatgccgccg
     1201 atgatgatcg ccgagaaaac cagcaacccg aaggaaacag ccggcagcaa ccagccggta
     1261 cggaagatcg tcgcaatgaa caaccctgcg cacagcaagg cgatgacgag caggatctcc
     1321 ttggcgggca gcacggcatg cacgtctgta taggacggcc cctggaccgc gccacggctc
     1381 gagaacgcca gaccgaatct gtcgaggtaa tacgcaatcg ccttgcagat gacgaacacg
     1441 aacagcagca acgcgaggtg ggtgcgcgca cccctggtga ctcgctcgcc aggcgtccgg
     1501 aaggccacgc cgccgaacac gtagtgcgag gcaagcgtca gcaacgtgca gaccacgatg
     1561 agagccatga cgtaaccgag aatgaatctc tggaacggca ggatgaatgc gaaataggag
     1621 acatcgcgat ggaactgcgg atccttgtgg ccgaaggatg tcgcgttgct ccacaacagc
     1681 caggtctgcc accgtcccga cgctgcggtc ccgaagatca gaccgaagaa ggcagcgatg
     1741 acgccgaata ccagccagcg gcgcggttcg atgacggtgc ggtagcggtc aagaatgatc
     1801 tgctcgactg acgaggctcg gaacgctggc cgcagccggt acgcgagata gacattgccg
     1861 aaggtcatcg ccgccatgaa gacgccgaag cccgcgaaca gggccaaccg ggtcaccagc
     1921 tttgtggtga agacctcgcc gaatccgacg gagtcgtacc acaggtgctc ggtgtcgatc
     1981 gcgacgaaca gtgcgatcgc aacgacaagg acgacgacga tcccgagcgt gatcaggagc
     2041 ggctttcccc aactcgggaa ctgcggccgc gggagcggct ggcgcggacc accgttaccg
     2101 gcgccgtacg gtcgccgctc ggggcgggga aacgtcacgc agacctcgat gtcctgcccg
     2161 gggaatgacc gggatcggcg ccagcggcga gggcacccaa tcgggccgcg ccgggcggca
     2221 acgccgccgt gcgatcaacc tacctaaagt ggcccaccag ggagcacatc gccgtccggc
     2281 aagatgtccc cgtgatcgag cccccggacg cgcagcctga cga
//
LOCUS       DATCBJ010000317         3199 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_213429, whole genome shotgun sequence.
ACCESSION   DATCBJ010000317 DATCBJ010000000
VERSION     DATCBJ010000317.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 3199)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3199)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3199
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_213429"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1035)
                     /locus_tag="VII50_06345"
     CDS             complement(<1..1035)
                     /locus_tag="VII50_06345"
                     /inference="COORDINATES: protein motif:HMM:NF003235.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HEY5050501.1"
                     /translation="MTTRWDLVQYDDVTRLHGPLMVVRGVQDVGWDEFAVIRIAGGPD
                     RHGLVLDVDGDLAVVEVLEGTQGMSSTSTSVSFDGHPLEISVGTSWLGRVCDGRGQPR
                     DGGPPVLGRRMVPVEGRPLNPTMRQPPTDPIITGVSAIDALTTLVRGQKLPIFSAAGL
                     PHLELAAQIAAQASVGNEPFSVIFAAMGLTHPDAAAVRAVLEARLSAGELVMLLNTAD
                     DPVIERLLTPRVALTIAEDLAFEHGRHVLVVMADMTSYAEALREVSAARGEIPGRRAY
                     PGYLYSDLASLYERCGRIRGVAGSVTVVPVLTMPAGDITHPVPDLTGYITEGQVVLSQ
                     EVHAHGVYPPV"
     gene            complement(1032..2759)
                     /locus_tag="VII50_06350"
     CDS             complement(1032..2759)
                     /locus_tag="VII50_06350"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF003220.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HEY5050502.1"
                     /translation="MNAPDDRARIVRVNGPLVEVADLVGVAVAELVEIGPHRLPAEVV
                     GISGSRSTLQAYEYTGGLAPGAGAWARGYPLSAPLGPWLLGGVFDGLLRPLKGGPTWL
                     GKGGLGASTDSAWSWEPLVKTGDAVAPGSLLGLIATGGALDYRVLAPVAVGGDVDFVS
                     EAGSHRADEELVRIGGVSVTMTDRWPVRQPRPCAERQDIDRPLVTGQRVLDMLYPVGR
                     GGSAAVPGGFGTGKTLLLQQVAKWCDADVIVYIGCGERGNEMADVLDEFADLTDPRTG
                     GRLADRTVIIANTSNMPMMAREASIYTGVTVAELFRDMGYHAVVIADSTSRWAEALRE
                     FASRSGVMPAEEGYPASLASSLAAFYERAGPVVTLGGLSGSVTVIGAVSPPGGDLTEP
                     VSALTQRFVRAVWSLDHDLAYARHYPAVSWSGSFSRDASTIASWYSREGDAGWEIRRA
                     LLSSILADSDRLTAFAELVGASSLPDKERVQLLAGRLVREGVLQQSSLVPNDAFCTPE
                     KAVALADATLGVINTCLELVDGGMSAADIEAFDFSTLLRAASDVPPDDIAEIGRRHDD
                     VVARLREAQ"
     gene            complement(2756..>3199)
                     /locus_tag="VII50_06355"
     CDS             complement(2756..>3199)
                     /locus_tag="VII50_06355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5050503.1"
                     /translation="EAAACVARTRSETATELDAARAAADQAVQDARRLGEAEAATAVA
                     IDDATVRRQVHTLLLVARRSVYEALRLHIRNDVRQLHGEPIYRDLRDTMTIAGRSLIG
                     PHATVRDVDDGCVIEASGRRVEVTLGSLADWALDGVLADSQEWTP"
BASE COUNT          568 a         1073 c         1018 g          540 t
ORIGIN      
        1 gacgggcgga tacaccccgt gagcgtgcac ttcctgcgag agcaccactt ggccttcagt
       61 gatgtaccca gtgaggtcag gcaccgggtg ggtgatgtca cctgccggca tcgtgagcac
      121 cggcaccacc gtgaccgagc cggccacgcc gcgtatccga ccacaacgct cgtatagcga
      181 cgcaaggtcg ctgtacaggt atccgggata tgcgcggcgc cccggaatct cccccctggc
      241 cgccgagacc tcccgcagtg cttccgcgta gctcgtcata tccgccatca cgacaagaac
      301 gtgccggccg tgctcgaaag cgagatcctc tgcgatggtg agtgcgacac gcggggtcaa
      361 caggcgttcg atgaccggat cgtctgcagt gttgaggagc atgacgagtt ctcccgcaga
      421 aagtcttgcc tccagcactg ctcggactgc ggcggcgtct gggtgggtca gtcccattgc
      481 ggcgaagatg acgctaaacg gctcgttacc gacagacgcc tgggcagcga tctgtgcggc
      541 gagctctagg tgcggcagcc cggcggcgga gaagatcggc aacttctggc cccgcacgag
      601 tgtggtcaac gcatcaatag cggacacccc ggtgatgatc gggtcggtcg gcggctggcg
      661 catcgtgggg ttcaatggac gtccctcgac cggcaccatg cgtcgtccca gtacgggcgg
      721 accgccgtcg cgcggctgcc cgcggccgtc gcacactctg ccaagccacg acgtgccgac
      781 cgatatctcc agcgggtgac cgtcgaacga caccgatgtg gatgtcgagc tcatgccttg
      841 ggtgccctcg aggacctcca ctaccgcgag atcgccgtcg acgtcgagaa ccaatccgtg
      901 tcgatctggt ccgccggcta ttcggatgac cgcgaattcg tcccagccga cgtcctgtac
      961 tccgcgcacc accatgagcg gcccgtggag gcgagtcacg tcgtcgtatt ggacgagatc
     1021 ccaccgggtt gtcactgtgc ctcccgcagt ctcgcgacga cgtcgtcgtg ccggcgaccg
     1081 atctcggcga tgtcatcagg cgggacgtcg ctagcagccc gcaggagcgt cgagaagtcg
     1141 aatgcctcga tgtcggctgc cgacatcccg ccgtccacca gttcgagaca ggtgttgatc
     1201 acgcccagcg tggcgtctgc gagcgcgacg gccttctccg gcgtgcaaaa agcgtcgttg
     1261 ggcaccagtg agctttgctg tagaacgccc tcacgcacca gacgcccggc gagcagctgg
     1321 accctttcct tgtcagggag tgaactcgca ccaaccagct cggcgaaggc tgtcaagcgg
     1381 tcgctgtccg caaggatcga cgacaggagc gctcgtcgaa tctcccatcc cgcatcgccc
     1441 tcgcgtgagt accaggaggc aatggtgctg gcgtcgcggg agaacgaacc cgaccagctg
     1501 accgctgggt agtgacgtgc gtaggcaagg tcgtggtcga gactccatac cgcccgcacg
     1561 aagcgctgcg tgagcgctga gacaggttcc gtgaggtctc cacctggtgg ggagacagcg
     1621 ccgattaccg tcaccgagcc ggagagcccg cccagcgtca ccaccggccc ggcccgctcg
     1681 tagaacgcag cgagggacga cgcgaggctg gcgggatagc cctcctccgc cggcatgacc
     1741 ccggaacgag aggcgaactc gcgtaatgct tcggcccatc gcgatgtcga atccgcgatg
     1801 acgacagcgt gatatcccat gtcgcgaaag agttcggcga ccgtgacacc ggtgtagatg
     1861 ctcgcttcac gcgccatcat cggcatgttc gaggtgttcg cgatgatgac ggtgcgatcg
     1921 gcgagacgcc cgccggtgcg tggatccgtt aggtctgcga actcgtcgag gacgtcggcc
     1981 atctcgttac ctcgttcacc gcacccgatg tagacgatga cgtcggcgtc gcaccacttc
     2041 gcaacctgct gaagcaacag cgtctttccg gtaccgaacc cgcccggaac ggcggcacta
     2101 ccgccccgcc caacgggata cagcatgtcc aggacccgtt ggccggtcac cagaggacgg
     2161 tcgatgtctt gccgttcggc acatggacgg ggttggcgaa caggccatcg gtccgtcatc
     2221 gtgacgctca caccgccgat gcgcaccagt tcctcgtcgg cccgatgtga tcccgcctcg
     2281 ctgacgaagt cgacatcacc gcccaccgcg accggcgcga gcacacgata atcgagcgcg
     2341 ccacctgtcg cgatcaggcc gaggagcgac cctggtgcaa cggcatcacc cgtcttgacg
     2401 agcggttccc acgaccacgc gctgtcagtc gatgcgccga ggccgccctt accgagccag
     2461 gtcggacctc ctttgagcgg gcgcagcaag ccgtcgaaaa cgccgccgag cagccatggt
     2521 cccaatggag cggagagtgg gtagccacgt gcccaggcgc cggcacccgg cgctagacct
     2581 cccgtgtact cgtacgcctg cagtgttgag cgagaaccgg atatgccgac gacttctgcc
     2641 ggtagccggt ggggcccgat ctctacgagc tcggccacgg ccacgccaac gagatcggca
     2701 acctcgacga gcggtccgtt gacccgcacg atccgcgctc ggtcgtcggg tgcgttcatg
     2761 gggtccactc ctgtgaatct gcaaggacgc cgtcaagtgc ccaatcggcg agtgatccca
     2821 acgtgacttc cacgcggcgt cccgacgctt cgatcacgca accgtcgtca acgtcgcgca
     2881 ccgtggcatg gggaccgatc agagatcggc cagcgatggt catcgtgtca cgcaggtcgc
     2941 gatagatcgg ttcgccgtgc aactgtctga cgtcgttgcg tatgtgcagt cggagcgctt
     3001 cgtagaccga tcgccgagcg accagcagga gtgtgtgcac ctgcctacgc accgttgcat
     3061 catcgattgc gacggcggtt gcagcttctg cttcgccgag gcgccgtgcg tcctgcacgg
     3121 cttgatccgc ggcggcgcgt gctgcgtcca attcggtcgc cgtctcactg cgcgtacgcg
     3181 cgacgcatgc tgccgcctc
//
LOCUS       DATCBJ010000318         2958 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_109063, whole genome shotgun sequence.
ACCESSION   DATCBJ010000318 DATCBJ010000000
VERSION     DATCBJ010000318.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2958)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2958)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2958
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_109063"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(7..2010)
                     /locus_tag="VII50_06360"
     CDS             complement(7..2010)
                     /locus_tag="VII50_06360"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEY5050504.1"
                     /translation="MPTKRPGINLVQLVASACLAVALLALSGVATWSAFATSDAASIS
                     TQASRLDDAYNSARFDIGAEESLERKYRLEPSADVRAQHLAGKTDLDAALAIVSRDGD
                     AADRKVAADVTERNATYLIATDLMFAATDQHNPATATTIDHNYVDPIFNTMQTIVYTQ
                     ATLHRNVDAKATRRLLSIESVARWATSLAVGVGAVLILLFGRLRRQSTKVHRAQSDLN
                     AYQATHDELTGLPNRALFATLLESALAQAREAQGSVAVVLLDLDRFKDINDTLGHRYG
                     DYLLQQIGPRIGAVLRDVDVLARLGGDEFVLLFPSHATGREAIHAALDLTRRILDALH
                     EPFMVDDVSLAVEASAGIAVWPAHGDTGDALLQHADIAMYLAKSRHEDLAIYDSALDG
                     HNPRKLTLLSQLRGAVDNGELVLNFQPLVDIGSGAVVGAEALVRWNHPDEGLLPPGEF
                     VPLAEGSGFIHELTRFVLRAACEQAKAWETAGQPLVVSVNISARCLLDTELPQSVAAT
                     LLVTGLPPHLLKLELTETAIIADPDRARSIIGRLHTLGVGLSIDDFGTGYTSLSFLRD
                     LPVQEIKIDRSFVTNMLMHPKDAIIVRTGVELAHRLGLHSVAEGIEDAATFAALAALG
                     CTTARGFHLGRPMPSGSFDTWLTEWNLSHSVSAAAHSGLPRAS"
     gene            complement(2032..2613)
                     /locus_tag="VII50_06365"
     CDS             complement(2032..2613)
                     /locus_tag="VII50_06365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5050505.1"
                     /translation="MHEHMSLRDRLEALTEHREPVFEPSDDPTLVAFSRLFAQSELFA
                     LEASKRIRADALSTIYDCETETRSDADAVPGIFEAIWTPVWPGEDPLTGFRTGSDSVF
                     GHRATVAMPQDERTAALLLLVTSTFVHLSASRAEPLPIIRGAMDAPGSTYRFVTLDPA
                     LGRAHVRAIRESRRAAARDSAQSDLSERGPAGR"
BASE COUNT          517 a          957 c          985 g          499 t
ORIGIN      
        1 cggcggctag ctggcgcggg gcaggcctga atgcgcagcg gccgacaccg aatggctgag
       61 gttccactcg gtcaaccacg tgtcgaaact gccgctcggc atcggccggc cgaggtggaa
      121 gcctcgcgcc gtcgtgcagc cgagcgcggc cagcgcggcg aacgtagcgg cgtcctcgat
      181 gccttcggcg acggagtgca gaccgagccg atgagcaagc tctacgccgg tgcggacgat
      241 gatcgcgtct ttcggatgca tcaacatgtt cgtgacaaat gaacggtcga tcttgatctc
      301 ctgcaccggg aggtcacgca ggaacgacaa cgacgtgtag ccggtaccga agtcatcgat
      361 cgacagcccg acgcccagcg tgtgcagcct gccgatgatc gaacgggcgc ggtcgggatc
      421 ggcgatgatc gccgtctcgg tgagttcgag tttgagcagg tgcggcggca gcccggttac
      481 cagcagcgtt gcggcaactg actgcggcag ctcggtgtcg agcaggcacc gcgccgagat
      541 attcaccgac accacgagcg gctgcccggc ggtttcccac gccttggctt gctcacaagc
      601 ggcgcgcagt acgaatcggg tgagctcgtg gatgaacccg ctaccttcgg cgagaggcac
      661 gaattcacct ggcggcagca gaccctcgtc ggggtgattc caccgcacca gcgcctcggc
      721 gccgacaacc gcgccgctgc cgatgtcgac cagtggttgg aagttcagga ccaactcacc
      781 gttgtcgact gcgccacgca actgcgacag cagcgtcagc ttgcgcgggt tgtgaccgtc
      841 gagcgcactg tcgtagatcg cgagatcctc gtgccgcgac ttcgcgagat acatcgcgat
      901 atcggcatgc tgcagcaagg cgtcgccggt gtcgccatgc gccggccaca ccgcgatgcc
      961 cgcgctggcc tcgacggcga gcgacacgtc gtccaccatg aatggctcgt gcagagcatc
     1021 gagaatccgc cgggtcagat ccaacgcggc atggatcgcc tcccggccgg tcgcgtgcga
     1081 cgggaacagc aacacgaact cgtcgccgcc caatctcgcc agcacatcga cgtcccgtaa
     1141 gacagcgccg atacgcggac cgatctgctg caacaagtaa tcgccataac ggtggccgag
     1201 tgtgtcgttg atgtccttga accggtcgag atcaagcagg accaccgcca ccgagccctg
     1261 cgcctcgcgg gcctgcgcta gtgcactctc gaggagcgtg gcgaagagcg cgcggttggg
     1321 caggccggtg agttcgtcgt gagtcgcttg ataggcgttc aggtcggatt gtgcgcggtg
     1381 caccttcgtc gattgcctgc gcaggcggcc gaacaacagg atcaggaccg caccgactcc
     1441 gacggcgagc gaggtcgccc accgcgcgac actctcgata ctgagcagac ggcgggtggc
     1501 cttggcgtcg acattgcgat gcagcgtggc ctgggtgtac acgatcgtct gcatggtgtt
     1561 gaagatcggg tcgacatagt tgtggtcgat agtggtcgcc gtagctggat tgtgctggtc
     1621 ggttgccgcg aacatcaggt cggtcgcgat caggtaggtg gcgttgcgtt cagtgacgtc
     1681 agccgcaacc ttgcggtcag ccgcatcgcc gtcccgactg acgatggcca gggccgcgtc
     1741 aaggtcagtc ttccccgcca ggtgctgtgc gcgaacgtcg gcgctcggct cgagtcggta
     1801 cttgcgctcc agggattcct cagcgccgat gtcgaagcgc gcgctgttgt aggcatcatc
     1861 aaggcggctc gcctgggtcg agatagaggc ggcgtcgctt gttgcgaatg ccgaccaggt
     1921 ggcgactcca gagagcgcga gcagagcgac cgccaggcat gccgaggcca ccagctgcac
     1981 caggttgatg cccggccgtt tcgtcggcat cgctgctccc ttgtcccggc cctatcggcc
     2041 agcaggcccc cgctctgaga gatcgctctg agcgctgtcg cgggccgcag cgcgccggga
     2101 ctcacggatt gcgcgaacgt gcgcccgacc cagcgctgga tcaagggtca cgaagcggta
     2161 ggtgctgccg ggggcgtcca tggcaccgcg aatgatgggc agcggttccg ctcgactcgc
     2221 ggacaggtgc acgaacgtgc tggtgaccag caacagcaga gccgccgtcc gctcgtcttg
     2281 aggcatcgcg accgttgccc gatggccgaa caccgagtcg gagccggtac gaaagccggt
     2341 caacggatcc tcgccgggcc agaccggcgt ccagattgcc tcgaagatcc ctggtacggc
     2401 gtcggcgtcc gaccgcgtct cggtttcgca gtcgtagatg gtggagagcg catcagcgcg
     2461 aatgcgtttg cttgcctcga gcgcgaagag ttccgactgc gcgaagagcc tgctgaaggc
     2521 gaccagcgtc ggatcgtcgc tcggttcgaa caccggctcg cggtgttctg tcagcgcctc
     2581 caggcgatcg cgcagtgaca tgtgctcgtg catggcgctc cttcctcacc gccatactca
     2641 cccatccgcg ccgatcgcgg gaccgcgagc ccgccgccgt ccgacaccac cccgccgtcc
     2701 gacctcgtgg cccattcctc agagattcac cgttccgcac gccgaaaccc gccaactcct
     2761 cacagacgca ggttttcttg cgctggagag ctgcttcatt gaggagccca cctgtatctg
     2821 tgaggaatcg aaggcggcgg aggcggggcg cggctccgcg ctaggccaac ggggctatcg
     2881 ccggcctcaa tgtctgcgcg ccaactgccg acgactaaag agacttcggg aggggttcgt
     2941 tggccatgaa cgcgaaca
//
LOCUS       DATCBJ010000319         2887 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_42454, whole genome shotgun sequence.
ACCESSION   DATCBJ010000319 DATCBJ010000000
VERSION     DATCBJ010000319.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2887)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2887)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2887
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_42454"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(174..638)
                     /locus_tag="VII50_06370"
     CDS             complement(174..638)
                     /locus_tag="VII50_06370"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEY5050506.1"
                     /translation="MTFRTESRLTIASSVDEVWAYMCDVRRWHEWAPTVLECRVRGGG
                     PFQLGAQLDQRAKGLFGLSRSRSQRVTVVEAPRHMAFAGPLGTSAGRWGMELEPVDDQ
                     QTDTMMWVEVDFAGLMRAIPAGAFKRRVQRVSDLEMVLIKSAVESDVSASTP"
     gene            complement(635..1318)
                     /gene="ribA"
                     /locus_tag="VII50_06375"
     CDS             complement(635..1318)
                     /gene="ribA"
                     /locus_tag="VII50_06375"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: protein motif:HMM:NF001591.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009231 - riboflavin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HEY5050507.1"
                     /translation="MHLSPPKATIRTQVTMPLRFVDGYTTPARVFTFNELVDGREHLA
                     LGLGTRARACTVDTGATVPLVRPHSECLTGDVFGSQRCDCGPQLREAVERIADAGGYL
                     LYLRQEGRGIGLYEKLDAYVLQDAGLDTYEANLALGHAADERDYTVAAQMLNALAVST
                     VALLSSNPDKAAQLTALGVTVTAQVPTHVHLSDANADYLAAKARWGAHLLETPMGINT
                     VALNTRAAR"
     gene            complement(1334..2026)
                     /locus_tag="VII50_06380"
     CDS             complement(1334..2026)
                     /locus_tag="VII50_06380"
                     /inference="COORDINATES: protein motif:HMM:NF013989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HEY5050508.1"
                     /translation="MADRPYTLLSCGMSIDGYLGSANMKPLQLSNDADFDRVDAVRAM
                     CDAILVGAETVRNDNPRLLIRSRSRQDERRARGQAPTPMKVTVTGRGRLDPCADFFAT
                     GNIEKVVYCASATVDDARARLGAVATIVDGGEPVDMHTISEDLYARGVQRLMVEGGGT
                     VHTQFLTADLADELHLVVAPFFVGDSRARRFVSDGLFPWNSRRRATLAEVRQIGDVVL
                     LRYALSARFRAD"
     gene            complement(2124..>2887)
                     /locus_tag="VII50_06385"
     CDS             complement(2124..>2887)
                     /locus_tag="VII50_06385"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025033.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEY5050509.1"
                     /translation="LVAEVRQLLPARRRAVVHDLGCGTGSMARWLAPQLSGPQHWVMY
                     DRDADLLARAALDVPPLTADGSPVAVETRQRDVTRLGQPDLTGASLITASALLDMLTA
                     AEVERIARSCVEAGCAALLTVSVIGDVALTPSDPLDDVIASAFNEHQRRADDDGRLLG
                     PDAVGAAADMFARLGADVVVAPSPWWLGARDRALTTEWFKGWVGAACEQRPELTVATQ
                     NYVEQRLADATAGRLRVTVHHHDLLAVPHEPPRDR"
BASE COUNT          484 a          973 c          938 g          492 t
ORIGIN      
        1 tgcgacgtcg atcacggtgt cacctgggcg aagccggagt gcttctacgg cccggaggcg
       61 ttgcccacgc tgggggtaac cgggcgcggg gtagagccgc gaggtgatgt cgtagtgctt
      121 cgccttcttg cggtaggtct cgatcaggtg ctcttgcgta cggtcgatct ggctcacggt
      181 gtgctcgccg acacatcaga ctcgaccgcc gacttgatca aaaccatctc gagatcgctg
      241 acgcgttgaa ctctgcgttt gaaagcgccg gcgggtatcg cgcgcataag tcccgcgaag
      301 tcgacctcaa cccacatcat cgtgtcggtc tgctgatcgt cgaccggctc gagttccatt
      361 ccccaacgcc ccgctgaagt acccagtggc ccggcgaacg ccatgtggcg gggcgcctcg
      421 acaacggtca cccgctggct ccgactgcga gacaacccaa acagcccctt cgcccgctga
      481 tccagctgcg cgccaagctg aaacggacct cctccacgca cccggcattc gagaacagtc
      541 ggcgcccact cgtgccagcg tcggacgtcg cacatatacg cccacacctc gtcgacgctg
      601 ctggcgattg ttaagcggct ctcggttcga aatgtcatcg ggcggccctt gtgttgagtg
      661 cgactgtgtt gattcccatg ggtgtctcga gcaggtgagc gccccatcgc gccttcgccg
      721 ccaggtagtc cgcattcgcg tctgacaggt gcacgtgcgt cggtacttgc gcggtcactg
      781 ttaccccgag cgcggtgagc tgggccgcct tatccgggtt gctgctgagc agtgccacgg
      841 tcgacactgc gagggcattg agcatctgcg cagcgaccgt gtagtcgcgc tcgtccgcgg
      901 cgtgcccgag ggcgaggttc gcctcatacg tgtcgaggcc cgcgtcctgg agtacgtacg
      961 catcgagctt ctcgtagagg ccgatcccgc gcccttcctg ccgaaggtag agcaggtagc
     1021 cgccggcatc tgcgatccgc tccaccgcct cgcgcagttg cggcccgcag tcgcatcgtt
     1081 ggctgccgaa gacgtcgcca gtgaggcact cgctgtgcgg ccggacgagg ggcactgttg
     1141 cgcccgtgtc gactgtgcat gcccgcgccc tggtgccgag gccgagcgct agatgctctc
     1201 gtccgtcgac cagctcgttg aaggtgaaga cacgcgcggg cgtggtgtac ccatcgacga
     1261 accgcaacgg catcgtcacc tgagtgcgga tcgttgcctt gggcggagat aagtgcacca
     1321 gacctcctcg atgtcagtcc gcgcgaaagc gcgccgagag tgcataacgc agcagcacca
     1381 cgtcgccgat ttgccgcact tcggccagcg ttgcccggcg acgcgaattc catgggaaca
     1441 gcccgtcact cacgaaccgc cgagcccgcg agtcaccgac aaagaagggt gcgacgacga
     1501 gatggagttc gtcggcgagg tcggccgtga ggaactgtgt atggaccgtg ccaccgccct
     1561 caaccatcaa tcgctggacg ccgcgtgcgt acaggtcctc gctgatcgtg tgcatgtcga
     1621 ccggctcacc gccgtcaacg atggtcgcca ccgcgccgag cctggcccgg gcgtcgtcca
     1681 ccgtcgcact agcgcaatag accaccttct cgatgttgcc ggtggcgaag aagtcagcac
     1741 acgggtcgag ccgaccacgc ccggtgacgg tgaccttcat cggcgtcggc gcctgtccgc
     1801 gagcgcgccg ctcatcctgc cgtgaccggg accgaatcag gagtcgtggg ttgtcgttgc
     1861 ggacagtttc ggcaccgacg aggatcgcgt cgcacatggc gcgcactgcg tcgacccggt
     1921 cgaaatcagc gtcgttggac aactggagcg gtttcatgtt cgcgctaccg aggtagccgt
     1981 cgatcgacat gccgcaactc aggagggtgt agggacgatc agccaccgat gctgcctcct
     2041 ccgggggcct gcccgacgcc gaccccgtgg ctacgcctct gagagttccc tgtgagtaca
     2101 tacgcacgca cggcgggtcc agttcaacgg tcacgcggcg gttcgtgcgg cacagccagc
     2161 aggtcgtgat ggtgcacggt cacccgcagg cgcccggcgg tcgcatcggc aagccgctgc
     2221 tcgacatagt tttgcgttgc gacggtgagt tcgggtcgct gctcacatgc cgcaccgacc
     2281 cagcccttga accattcggt cgtcagtgcc ctatccctgg cgccgagcca ccaggggctt
     2341 ggcgcaacga ccacgtccgc acccaatctc gcgaacatgt cggctgccgc gcccacggcg
     2401 tccgggccga gcagccggcc atcatcgtca gcccggcgtt ggtgttcgtt gaaagccgac
     2461 gcaatcacgt catccagtgg gtcggacggc gtgagggcga cgtcgccgat gaccgagacg
     2521 gtcagcaatg ctgcgcaacc tgcctcgacg cacgaacggg cgatccgctc gacctcagcg
     2581 gcggtcagca tgtcgagcaa tgccgacgcg gtgatgagcg acgcaccggt gaggtcgggc
     2641 tggccaaggc gcgtgacgtc acgttgccgc gtctcgacgg ctaccggcga gccgtcggca
     2701 gtcaacggcg gaacatcgag ggccgcgcgg gcgagcagat cggcgtccct gtcgtacatc
     2761 acccagtgct gcggcccgga aagctgcggg gcaagccacc gcgccatcga gccggtgccg
     2821 cagccgagat cgtgcaccac ggcccgacgg cgcgccggca ggagttgccg gacctccgcg
     2881 accaaat
//
LOCUS       DATCBJ010000320         3763 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_368478, whole genome shotgun sequence.
ACCESSION   DATCBJ010000320 DATCBJ010000000
VERSION     DATCBJ010000320.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 3763)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3763)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3763
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_368478"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..695)
                     /gene="thyX"
                     /locus_tag="VII50_06390"
     CDS             complement(<1..695)
                     /gene="thyX"
                     /locus_tag="VII50_06390"
                     /EC_number="2.1.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050797 - thymidylate synthase (FAD)
                     activity [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent thymidylate synthase"
                     /protein_id="HEY5050510.1"
                     /translation="MSQTPGAGVALPETAADALQPRFRSDVTVELVKHLASDADVLFA
                     ARVSTKGESSLDDVHADPSRSQGLLNYLMRDRHGTPFEHNSMTFFVSAPIFVFREFMR
                     HRVGFSYNEESGRYRQLEPVFYVPGPDRLLVQDGKPGRYVFTAGTAEQHAVAERATKA
                     ACEQAHAAYLEMLAAGVAREVARGVLPVATFSSMYVTCNARSLMALLSLRTKHEDSRF
                     PSFPQREIEMVAE"
     gene            complement(710..955)
                     /locus_tag="VII50_06395"
     CDS             complement(710..955)
                     /locus_tag="VII50_06395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5050511.1"
                     /translation="MNGYVSQLIAEGHIAELRAEAAQRRLRTQAAQAGATRTPRKSTG
                     WPARLRLDAESARRARPFAAGPGGRVTTVRVQPTVDC"
     gene            1104..1670
                     /locus_tag="VII50_06400"
     CDS             1104..1670
                     /locus_tag="VII50_06400"
                     /inference="COORDINATES: protein motif:HMM:NF024898.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY5050512.1"
                     /translation="MPTPAGPHADGSVRVATADDAATIAGIQAEALRLNYSALLPEAA
                     INEFDLAAATNGWTTAISSPPSPRHRVFVAVDLAGVVGFAASAPATDADLDAERDAEL
                     PALHVAPGHTRLGHGSRLMAAIVDHAHDDGVNRLVTWVFAADDPMRMFLRDNGWEPDG
                     STRDLDVGELLHQVRMHTGIKDSPDLIA"
     gene            complement(2401..3471)
                     /locus_tag="VII50_06405"
     CDS             complement(2401..3471)
                     /locus_tag="VII50_06405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011728598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
                     /protein_id="HEY5050513.1"
                     /translation="MSLTVVVQMPAEDRAELTRWTRMSSLQAGLAQRARIVLLADEGV
                     GTNEIVHRVGVSKPTVIGWKKRYAANGIAGLDDRPKPGRPASIDEVAVVLATLEPPPE
                     TLGVTHWSSRLLGHQLGISNVWVARIWRKWGLQPWRRETFKFSTDPQLEAKIRDVVGL
                     YLNPPEKAIVLSIDEKSQIQALDRTAPILPMRPGLPEKATHDYVRHGTTTLFAALEVA
                     TGKVTDACYPRHRHEEFLRFLKQVAKAYPRVPLHLVVDNYATHNHPQVKAWLARNPRI
                     TMHFTPTSGSWLNMVEIFFGIITRQAIRRGTFTSVKDLIAAIKTYIDAWNERCQPFTW
                     TKTADDILTKAASGQRSSFTRH"
     gene            complement(3502..>3763)
                     /locus_tag="VII50_06410"
     CDS             complement(3502..>3763)
                     /locus_tag="VII50_06410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5050514.1"
                     /translation="ALVLSFELLMRQLRQQLSSNPVPQLPGTDLHLATSAAPAARLTN
                     EPARTAGRQNLLDKARHIHADQERRGTRLTGKTLAALLVSRE"
BASE COUNT          623 a         1229 c         1220 g          691 t
ORIGIN      
        1 ttctccgcaa ccatctcgat ctcgcgctgc ggaaaactcg ggaaacggct gtcttcgtgc
       61 ttcgtgcgaa gcgacagcag tgccatcagc gatcgggcgt tgcaggtgac gtacatcgac
      121 gagaacgttg ccaccgggag caccccacgc gcgacctcgc gcgcgacgcc agcagcgagc
      181 atctcaaggt atgcagcgtg ggcctgctcg cacgctgctt tggtcgcccg ctcggcaacc
      241 gcgtgttgct ccgcagtgcc ggcggtaaag acgtagcgcc cgggcttgcc atcctgcacg
      301 aggaggcggt cgggcccggg cacgtagaac accggctcga gctggcggta gcggccactc
      361 tcctcgttgt agctgaaacc cacccggtgc cgcatgaact cgcggaacac aaagatcggc
      421 gcggaaacga agaacgtcat cgagttgtgc tcgaacggcg tgccgtggcg atcgcgcatc
      481 aagtagttca gcaaaccctg cgaccgcgaa ggatcggcat ggacgtcgtc gagcgacgac
      541 tcgcccttcg tcgagaccct agctgcgaac agcacgtcag cgtcgctcgc cagatgcttg
      601 accaactcga cggtgacgtc actacgaaac cgtggctgca atgcgtcggc ggcggtctct
      661 gggagcgcga cccccgcacc tggtgtctgc gacatgacgg acacgatagt cagcagtcga
      721 ccgtgggctg gacgcggaca gtcgtgaccc ggccgccggg cccggcggca aagggacggg
      781 cgcggcgcgc ggactcggcg tccagtcgca accgcgcagg ccagccggtt gacttccgcg
      841 gcgtgcgggt cgcccccgcc tgcgccgcct gcgtccgcag cctccgctgg gcggcttcgg
      901 ccctcagttc cgcaatgtga ccctcggcta tcaactggct cacgtacccg ttcatcgcct
      961 gctcctctgc ggactttcct gagcgccgct cgtgcgatcg ctcacaatca gttctatccg
     1021 cggctactga cactttccgt cggccgacgc tccccgccgc cggccctgcc ccggccctcg
     1081 ggcgctgctg cgaggatggc gccatgccca cccccgcagg gccgcacgcc gacggcagtg
     1141 tgcgcgtcgc cacggccgat gacgcggcca cgattgccgg gatccaggcg gaggcgttgc
     1201 gcctgaatta ctcggcgcta ctacccgagg ccgcgattaa cgagttcgac ctcgctgccg
     1261 caaccaatgg gtggaccacc gccattagca gtccgccgtc gccgcgccac cgagtgtttg
     1321 tggcggtcga cctggcgggt gtggttggtt tcgctgcgtc agcgccggcc acggatgccg
     1381 acctcgatgc cgagcgggac gccgagttgc cggcgctgca cgtcgctcca ggacacaccc
     1441 gtctcgggca cggcagcagg ctcatggcgg cgattgtcga tcacgcgcac gacgatggag
     1501 tcaaccggct cgtcacgtgg gtcttcgcgg ccgacgaccc gatgcggatg ttcctgcgcg
     1561 acaacggttg ggagccggac ggatcgacac gcgacctcga cgtcggcgaa ctgctgcacc
     1621 aggtccggat gcacaccggc atcaaagact cgcccgacct gatcgcctag ccgcagccgg
     1681 ttggctaatc ccgtccggcg gagcattgat gcccagcgct cgcaagttcg agcggatacc
     1741 aacaccgcgt aacggacgct cctctccgac caccacgccg aatagcgccc tttgacatca
     1801 caggtgtcaa tggcgaaggc actcatttgc cgagatccgg taaatgcggc agtgagtgcc
     1861 ggctacggcc ggtaccgaac gcgacgatcc ggtgacagcc gcattgacag acgggcgtgt
     1921 tccatggtcg ctggcgaggg cacggctgaa gatctcctga cgtctcagtc ggtttccgac
     1981 accaacgcga gatcgcaggc tgtgcagtgc atcggggtgg accttgccag ctcttggcga
     2041 gcctgccacg tggcgtatat cagctgtcgg ctatagatcc aagtactacc tctcgggcgt
     2101 tctgactgtt accgtcccgc gcaggtcggc atgcaccgtc tgcgcagcgg gggcgttggt
     2161 gacctgtttc gacaccggac gccgctttgg cggccgcgtg ccaaaccgcc ggtcgccccg
     2221 tcgcgacccg tccttgattg gagtgagcac ggcaccctcg cgcaaccttc gcaccgctcg
     2281 caatccgagc ggccggactt gggaagcctt gcgcgcgtat ttcatccatc gtcaaacgtg
     2341 tggcttacgt ggttgccgcc gtagcactgg caattgggtt caccgccgga agcgcggcgg
     2401 ctagtgtcgc gtgaacgatg acctttgacc gctggcggct ttggtgagga tgtcgtcggc
     2461 ggtcttggtc caggtgaatg gttggcagcg ttcgttccag gcgtcgatgt aggtcttgat
     2521 cgcggcgatg aggtctttga ctgaggtgaa cgtgccgcgg cggatcgctt ggcgggtgat
     2581 gatcccgaag aagatctcga ccatgttcag ccacgatccc gatgtcgggg tgaagtgcat
     2641 cgtgatccgc gggttgcgcg ccagccacgc tttgacctgc gggtggttgt gggtcgcgta
     2701 gttgtcgacg accagatgca gcggcacccg cgggtaggcc ttcgcgactt gcttgaggaa
     2761 acgcaagaac tcctcgtgac ggtggcgtgg gtagcaggcg tcggtcactt tcccggtcgc
     2821 gacttccaag gcggcgaaca gtgtcgtggt gccgtggcgg acgtagtcat gggtcgcttt
     2881 ctccggcaac cctgggcgca tcggcaggat cggtgcggtc cggtcgagtg cttggatttg
     2941 ggacttctcg tcgatcgaga gcacgatcgc cttctccggc gggttcagat acaggcccac
     3001 gacgtcacgg atcttggctt ccagctgggg atcggtggag aacttaaacg tctcccgccg
     3061 ccacggctgc agtccccact tgcgccaaat cctggcgacc cacacgttcg aaatcccaag
     3121 ctgatgaccc aacagccgag acgaccagtg cgtgacaccg agtgtttctg gcggcggctc
     3181 gagcgtcgcg agtaccaccg cgacctcgtc gatgctcgcc ggccggccag gcttcggccg
     3241 atcatccaat ccggcgatcc cgttcgcggc gtagcgtttc ttccacccga tcaccgtcgg
     3301 cttggacacc ccgacgcggt gcacgatctc gttcgtcccc acaccctcat cggctaacaa
     3361 cacgatccgc gcgcgctgcg cgaggcccgc ctgcaacgac gacattcggg tccaccgggt
     3421 cagctcggca cgatcctcag ctggcatctg cacgacaaca gtcaacgaca tgaacacagg
     3481 ctaccgcgag gtaaagctat ttcattcacg cgacactagg agcgcggcga gtgtttttcc
     3541 ggtgagtctg gtgccgcggc gttcttggtc ggcgtggatg tgtcgggctt tgtcgagcag
     3601 gttttgtcgg cccgctgtgc gagccggctc gttcgtcaat cgcgccgcgg gtgcggcgct
     3661 ggtggcgaga tgcaagtcgg tacctggtag ctgcggcacc gggttgctgc tgagttgttg
     3721 gcgtaattgt cgcatgagca gttcgaacga caacaccagt gcc
//
LOCUS       DATCBJ010000321         2840 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_43076, whole genome shotgun sequence.
ACCESSION   DATCBJ010000321 DATCBJ010000000
VERSION     DATCBJ010000321.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2840)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2840)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2840
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_43076"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..386)
                     /locus_tag="VII50_06415"
     CDS             complement(<1..386)
                     /locus_tag="VII50_06415"
                     /inference="COORDINATES: protein motif:HMM:NF024212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEY5050515.1"
                     /translation="MTTAGGRTGATRAVARVPAPRLTYLVKQAELAIRAHLDRIVGRH
                     GLTTLQYTALSVLERNPGISSAQLSRRSFVTAQAGNEMVAILERKGLIERQPSPAHLR
                     IRLVYLTAAGAALLDSCDAEVDALEA"
     gene            487..1311
                     /locus_tag="VII50_06420"
     CDS             487..1311
                     /locus_tag="VII50_06420"
                     /EC_number="4.1.2.61"
                     /inference="COORDINATES: protein motif:HMM:NF006588.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxycinnamoyl CoA hydratase/lyase"
                     /protein_id="HEY5050516.1"
                     /translation="MEGTWSTVAVEIEDGIGWVSLNRPDKRNAMSPTLNREMIAVLQE
                     LEGDDRVRVLVLTGAGDAWSAGMDLKEYFRDVDASPPHVQTQARRDSAEWQWRRLIHF
                     SKPTIAMVNGWCFGGAFTPLVCCDLAIAADEAIFGLSEVNWGIPPGGLVSRALAETVS
                     SRDALWFIMTGETFDGVRAAQMRLVNESVPLAHLRERTTEVAQKLAGMNLAVLRAAKI
                     GFKKARLMSWDEAEDYLYAKLDQAVLGDPESGKEQGLAQFLDEKTLRPGLDSYNRV"
     gene            1332..>2840
                     /locus_tag="VII50_06425"
     CDS             1332..>2840
                     /locus_tag="VII50_06425"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEY5050517.1"
                     /translation="MIYRSLYPDPVIPRTSLPEYVLAAAASQSARPALIDGSTGETLT
                     YGDLMRLVPAIAQSLASMPVQPGDVVALIGPNRPAWAAAFYGALAAGATITALSPQLT
                     EREVSRQLRESRAVALIADASVTSVAQAAGVSIAIPVLDLTALCDTAIATDGHERLSA
                     NPESDVAVLAYSGGTTGLPKGVLLTHHNLVACLCQHEAVYHVDSNDVFLAALPFFHIY
                     GMSIILGYGLRHGATIVTMPRFDADRYLTLIAERRVTRLHLAPPLAAVVAAAGPAFDL
                     TSVRHAMSGAAPLDAATVARLEARLGCPIGQGYGMTEASPGITWVPDVGAGSPAGSVG
                     HLLPGTEGRVVDSDSLTDATGPGELWIRGPQVMRGYLANPTATENAIVEGGWLRTGDA
                     VRIDESGVVWVVDRLKEIIKYKGYQIAPAELEAVLLEHPSVADVAVIGVADAVAGEIP
                     KAFVVRKEIDLTDAALIAWCAQRVAPYKRPRAVEFVASIPRSTSGKVLRRMLR"
BASE COUNT          507 a          901 c          924 g          508 t
ORIGIN      
        1 ctcgcctcaa gtgcgtccac ctccgcgtcg cacgaatcca gcagcgctgc gccggccgca
       61 gtgaggtaga cgagccggat gcgaaggtgt gcagggctcg gctgtcgctc gatgaggccc
      121 ttgcgctcca agatggcgac catttcattg ccggcttgtg cggtgacaaa tgatcgccgc
      181 gacagctgcg ccgacgagat ccccgggttg cgttcgagca cactcaatgc cgtgtactgc
      241 agggtcgtga gcccgtgtct gccgacgatg cgatcaaggt gcgcccggat ggcgagctcg
      301 gcttgcttaa ccagataggt caaccggggc gcgggaacac gagcaaccgc gcgtgtcgca
      361 ccagtgcgtc cgcccgcggt cgtcataccg acacggtagc aacgaaagat tgccccgagg
      421 gattgtggtc tcgcgccgta cgaggcacac tgccatatca aggtacctga tattggaggg
      481 acgatggtgg agggcacctg gtcaacggtc gcggtggaga tcgaggacgg catcgggtgg
      541 gtttcgctga atcgtccgga taagcgcaac gcgatgagcc cgacgctgaa ccgcgaaatg
      601 attgccgtcc tgcaggagtt ggagggcgac gaccgagtgc gggtcctggt actcaccggt
      661 gcaggcgacg catggtcggc tggcatggat ctcaaggagt acttccgcga cgttgacgcg
      721 agccccccgc acgtgcaaac ccaagcgcga cgcgacagcg ccgagtggca atggcgtcga
      781 ctgatccact tttcgaagcc gacgatcgcg atggtgaacg gctggtgttt cggcggggcg
      841 ttcacaccgc tcgtgtgttg cgatctggcg attgccgcgg atgaagcgat ttttggtctc
      901 tcagaagtca actggggtat tccgcccggc ggcttggtga gtcgagcgct cgccgagacc
      961 gtctccagtc gcgacgcgct gtggttcatc atgaccggcg aaacgttcga cggagtccgc
     1021 gcagcccaaa tgcggctcgt caacgagtcg gtgccactcg cgcacttgcg cgagcgcacc
     1081 actgaggtgg cgcagaagct ggccggcatg aacctagccg tgttgcgagc cgccaagatc
     1141 gggttcaaga aggcacgact gatgtcgtgg gatgaggcag aggactacct atacgcgaaa
     1201 ctcgaccagg ccgttctcgg tgacccggag agtggcaagg agcaggggct tgctcagttc
     1261 ctcgacgaga agacattgcg cccgggcctc gactcctaca accgggtgtg agcggcccag
     1321 ggatcccgag catgatctac cggagcctct acccagatcc cgtcatcccg cgcaccagcc
     1381 ttcctgagta cgttcttgcg gccgccgcaa gccagtcggc acgtcccgcg ctgatcgacg
     1441 gaagcaccgg cgagacgctg acgtacggcg atctcatgcg gcttgtcccg gcgatcgcgc
     1501 aatctctcgc atcaatgccg gtgcagccag gtgacgtcgt ggcgctcata ggtcccaacc
     1561 ggcccgcctg ggcggcggcc ttctacggcg cactcgcagc cggcgccacg atcaccgcct
     1621 tgagcccgca gctaaccgaa cgggaggtga gccgtcagct gcgcgagtca cgtgcagttg
     1681 cgttgatcgc cgacgccagc gtgacctcgg tcgcgcaggc agcgggcgta agcatcgcga
     1741 tccccgtcct cgacctcacg gccctttgcg acacagcgat cgccactgac gggcacgaac
     1801 ggctttctgc caatcccgaa tcggacgtcg cggtgctggc gtactcgggc ggcaccaccg
     1861 gcttgccgaa aggagttctg ctcacccacc acaacctcgt cgcgtgccta tgccagcacg
     1921 aggcggtcta tcacgtcgat tcgaacgatg tctttcttgc ggcgctgccg ttcttccaca
     1981 tctacggcat gtccatcatc ttgggttatg ggcttcgcca cggcgcgacg atcgtcacca
     2041 tgccgcgttt cgacgctgat cgatacctca cgctgatcgc cgaacgacgc gtcactcggc
     2101 tccacctggc gccgcccttg gccgctgttg ttgcagcggc cgggccggcg ttcgacctga
     2161 cctcagttcg ccacgcgatg tcaggtgccg caccgctcga cgccgcgacg gtggctcggc
     2221 tcgaggcccg tctggggtgt ccaatcggtc aggggtacgg catgaccgaa gccagtccag
     2281 gcatcacgtg ggtccccgac gtcggagcag gatctccggc cggttcggtc ggccatctgt
     2341 tgccaggcac cgaaggccga gtcgtcgact cggattcgct cactgatgca acgggacccg
     2401 gggaactttg gattcgcggg cctcaggtga tgcgcggata cctcgcaaac ccaactgcca
     2461 cggagaatgc gattgtcgag ggcgggtggc tgcgcaccgg cgatgccgtg cgcatcgacg
     2521 aatcgggagt cgtctgggtc gtcgatcggc tcaaggaaat catcaagtac aagggctatc
     2581 agatcgcccc cgccgagctc gaggccgtgt tacttgagca tccatcggtc gcggacgtcg
     2641 ccgtcatcgg ggtggcagac gctgttgcgg gtgagatccc caaggccttc gtcgtgcgca
     2701 aagagatcga tctgaccgat gccgcactta tagcgtggtg cgcgcaacga gttgccccgt
     2761 acaagaggcc gcgcgccgta gagtttgtcg cgtcgatccc caggtcgaca tcaggaaagg
     2821 tcttacgccg catgctccga
//
LOCUS       DATCBJ010000322         2422 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_184480, whole genome shotgun sequence.
ACCESSION   DATCBJ010000322 DATCBJ010000000
VERSION     DATCBJ010000322.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2422)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2422)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2422
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_184480"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2176)
                     /locus_tag="VII50_06430"
     CDS             complement(<1..2176)
                     /locus_tag="VII50_06430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5050518.1"
                     /translation="MTVDDPLATDEPDDFDAEVLVDVQTGRSDASRRLPGRILALASV
                     VPAVVAAAWVAGALPLLALHIYTPVPGTILALAIAVVLAHPVLRVTLAAADRFGDVPW
                     WALIAVLAVVVLAGGLAFAHSAEDVLVRRDPGSYAMSATWLSSHGTIQMPSHPGLFGA
                     PNSDLVLASQGFYQQGSHIIPQFMTGVPVLLSIGGWMAGLSGVLHGNAFIGALALLAF
                     AGVATRLVGARWAPLAVLALAFVQPELDVMRATYSEPAGQLILLGGLAIVVDAFVAGE
                     LVPHLWPRRASAQSDAPAVAPKVAARGLFVGGLVLGLVFVVRIDAVADLFPLVPLFGW
                     LAFHRVVGSRWFAGGLLLGLAFGGFDCLVLTYPYAQHVGSDLAAVAYGFAVLVPLTFA
                     FLVAARRRQLRDAAGVPPKPAAGRWRVAIAAAALFSVGLAFPLVALARALRRFLPILA
                     AAGVFVIGLFLFLRPHLMTMRANPESGGANYVEQVQRFLHMPVDPTRSYYEQATRWLS
                     WYVGWGTLALALIGACWLAYEQIAGRRRVWAPAFLVFFGTATAVLLLPSITPDHPWAD
                     RRFVPVVLPGLVLFAIMAVVPAVQFLAGRSARSWSAGSGGARSTLLGASQGAVAALAV
                     LLIVVPVWTGSREVIYTKTEVGEPTLVHQVCAQLRPGDVVIAIGSRARTEWPGTMKVM
                     CGVNVGYLDNDDDLTEMAQIDTQVQADGGRLMLLAESVGDKNA"
     gene            complement(2173..>2422)
                     /locus_tag="VII50_06435"
     CDS             complement(2173..>2422)
                     /locus_tag="VII50_06435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5050519.1"
                     /translation="ASNELAPHLVDRTKVMLLDATPHDAPWVVFDTGYIEFPMTEQAQ
                     QTRSSWLLSHGYQQVFSRDSIVVYHRVGAAGGTAEGAP"
BASE COUNT          488 a          846 c          795 g          293 t
ORIGIN      
        1 ccgcgttctt gtcaccaacg ctctcggcca gcagcatcag ccggccacca tcagcctgca
       61 cctgcgtatc gatttgtgcc atctcggtca ggtcgtcgtc gttgtcaagg tagccgacat
      121 tcaccccgca catgaccttc atcgtgcccg gccactcggt ccttgcccgg ctaccgatgg
      181 cgatgaccac gtcacccggc cgcagctggg cgcacacctg atgcaccagc gtcggttcgc
      241 cgacctcggt cttcgtgtag atcacctccc gactgcccgt ccagaccggg acgacgatga
      301 gcagcacagc gagcgcggcg accgctcctt gcgacgctcc gagcagcgtg gatcgcgcgc
      361 cacccgagcc cgccgaccac gagcgggcgg agcggcccgc gaggaactgg actgcgggaa
      421 cgaccgccat gatcgcgaag agcaccagcc ctgggagcac caccggaaca aagcggcggt
      481 ccgcccacgg gtggtcggga gtgatgctcg gcagcagcag aaccgctgtc gccgttccga
      541 agaagaccag gaatgcgggc gcccacaccc gtcggcgtcc ggctatctgc tcataggcga
      601 gccagcaggc gccgatcaag gcgagcgcca acgtccccca cccgacgtac cacgacaacc
      661 atcgggtcgc ttgctcgtag tacgaccgcg tcgggtcgac gggcatatgc aagaaccgct
      721 gcacctgctc gacgtagttc gcaccgcccg actccgggtt ggcgcgcatc gtcatcaggt
      781 gcggccgcag gaagaggaac aggccgatca cgaacacgcc ggcggccgcc aggatgggca
      841 agaatcggcg caacgcacga gcgagagcga ctagcggaaa cgccagcccg acggagaaca
      901 gcgccgccgc agcaatcgcg acgcgccagc gcccggcggc gggttttggc ggaactcccg
      961 cggcgtcacg cagctgccgc cgtcgtgccg cgacgaggaa ggcgaacgtc aacggcacca
     1021 agacagcgaa gccataggcg accgccgcca ggtcggaccc gacatgttgc gcatacggat
     1081 aggtaagcac caggcagtcg aacccgccga aggcaaggcc gagtagcagc ccgcccgcga
     1141 accagcgcga cccgacgacc cgatggaacg cgagccagcc gaaaagcggt accaagggga
     1201 acaggtcggc gaccgcatcg atgcgcacca cgaacaccaa gccgaggacg aggccgccca
     1261 cgaagagccc tcgagcggcg actttcggag cgactgcggg cgcgtcgctc tgggcgctcg
     1321 cccgccgtgg ccatagatgg ggaacgagct cacccgcgac gaaagcatcg acaacaatcg
     1381 ccaggccgcc gaggaggatc agctggcccg cgggttcgct gtaggtggcc cgcatgacgt
     1441 caagctcggg ctgcacaaac gcgagtgcga gcaccgcgag cggagcccac cgcgcgccga
     1501 caagtcgggt ggccacgccg gcgaacgcca gcagtgcgag cgcaccgatg aacgcgttcc
     1561 cgtgcagcac gccgctcagc ccggccatcc agccgccgat cgacagcagc accggcactc
     1621 cggtcatgaa ctgcggaatg atgtgcgacc cctgctggta gaaaccctgc gaagccagca
     1681 ccagatcaga attcggcgcg ccgaacagcc ccggatgcga gggcatctgg atcgtgccgt
     1741 ggctcgacag ccaggtcgca ctcatcgcgt acgaacccgg atcacggcgg accaagacat
     1801 cttcggcgct gtgggcgaat gcgagaccac ctgccaggac aacgacagcg aggacggcga
     1861 tcagtgccca ccacggcacg tcgccgaatc ggtctgcggc tgcaagcgtg acgcggagca
     1921 ccggatgcgc gaggacgacg gcgatcgcga gagcaaggat cgtgccgggc accggcgtgt
     1981 agatgtgaag cgcgagcagc ggcaatgcac cggctaccca ggctgccgca accacggccg
     2041 gcaccacaga agcaagcgcc aggattcggc cgggaaggcg gcgcgacgca tccgaccgcc
     2101 cagtctggac atcaacgagc acctcggcgt cgaagtcgtc cggctcgtca gttgccaacg
     2161 gatcgtcgac ggtcacggcg caccctcagc cgtgccgccg gccgcgccga cgcggtgata
     2221 cacgacgatc gagtctcggg agaacacctg ctgatatccg tgcgacagca gccagctgct
     2281 gcgggtctgc tgcgcctgtt cggtcatcgg aaactcgatg tagccggtgt cgaacacgac
     2341 ccagggtgcg tcgtgcggcg tcgcatcgag cagcatcacc ttggtgcggt cgaccaggtg
     2401 cggcgccagc tcgttgctgg cc
//
LOCUS       DATCBJ010000323         3831 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_442108, whole genome shotgun sequence.
ACCESSION   DATCBJ010000323 DATCBJ010000000
VERSION     DATCBJ010000323.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 3831)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3831)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3831
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_442108"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1010
                     /locus_tag="VII50_06440"
     CDS             <1..1010
                     /locus_tag="VII50_06440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5050520.1"
                     /translation="FVAGDGGIFPFPWAGFVGQIAIVAIGLTVAPLYERVVRRTLWIY
                     GASCVVLFFIPNPVGGNIGRLAGIMIGPAAAFVLLRAHRKTALLVVAAPLLIFQLLPV
                     IGAISSAAGDPSTKAQYYQGMLSFLTQNQQPLGRVEIPFTRDHWEAKYVAEKVPLARG
                     WDRQIDLSRNAVLYAPLTVATYHTWLFENAVRFVALPDAPLDQGGQAEKALLRHPPSY
                     LRLVYADNQWHIWSVVGAAPLASGAATVTALTPSSFTMNASTGGVTFVRLRWSAYWHV
                     DNAAPACVAPAGGGWTTVLSFAPGTVKISARALPGDTRCTDLQLARFGLGDAGAPEPT
                     Y"
     gene            complement(1042..2223)
                     /locus_tag="VII50_06445"
     CDS             complement(1042..2223)
                     /locus_tag="VII50_06445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5050521.1"
                     /translation="MSRDHRDGRDGITDVDRLDAIVRTGAARVLDRAWNESAGYCKPN
                     RRSYPHLWLWDSCFHAIAWFALGDARGSREIEAVFTGQLPDGFLPHMRYGRRTYPRGP
                     LKGVSSFTQPPVYARALKAGADAGFPASDELLDCAQRALAALWRDRLRDGLLVIVHPW
                     EAGTDDSPRWDSWVGASVWNRRVWTAFDRALLKRTVFNPSGHAIDNPDFVVAPASFNA
                     IAADAATTLGELIGDAAWIARGRALAATFDDAAWDADEALWSDVTFTGGGVSVRIPTM
                     DAALPAMCTPDPAKAAAALRQLTDPARFGAPYGPRFVPKSQPTYNPMQYWRGPAWPQL
                     NYLAAYSARQCGDGALATEIGRLTKRACLRAGFAEYWNPETGRGLGARPQSWAAVAAA
                     M"
     gene            complement(2220..2804)
                     /locus_tag="VII50_06450"
     CDS             complement(2220..2804)
                     /locus_tag="VII50_06450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5050522.1"
                     /translation="MTGDQSADRADVLITGGRESPRRLPRFLVPVLCVVVLIAYGAVA
                     LDRDSRHNEFKTLLGATINGQATAAHADATLESTRQYTMPLLGSAPANVRAGLEQLID
                     QSASQGVAKVRATRAAVASTSVLPWHTALHKAKAAELAYLDEWTAYLDAVAHGADTGT
                     IPTTVLQAKQTGAAAALRDAAPDHKASLAIPSSL"
     gene            complement(2822..>3831)
                     /locus_tag="VII50_06455"
     CDS             complement(2822..>3831)
                     /locus_tag="VII50_06455"
                     /inference="COORDINATES: protein motif:HMM:NF016047.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cell wall-binding repeat-containing protein"
                     /protein_id="HEY5050523.1"
                     /translation="LTTLASPSVSVGSGGGGGTSGGGTSGGGGGAGSAAQALTLGRVA
                     GADRIATSVAASQTGFPTAHSAATVVLARSDTFADALAGTPLAVGLHGPLLLTASASL
                     STATAAEIQRVLAPGATVYMLGGISALSPAVANAVAALGHPVVRVAGSDRFATAVQVA
                     AKLGNPITVFEADGTNFPDALSAGSAAAATGGVVLLTAGSGQSGPTAAYLAAHPSAQR
                     YAIGGPAAHADPLAKAFVGSDRFATSVLVAQAFFPTPAAVGLASGLTFPDALSGGSVA
                     AMNHGPIVLVPSSGTLPSSVAGYLETAQSSATSAWLFGGTSSVGADIFSAAAAILDPA
                     A"
BASE COUNT          599 a         1284 c         1313 g          635 t
ORIGIN      
        1 ccttcgtcgc cggcgatggt ggcatcttcc cgtttccgtg ggccggattc gtcgggcaga
       61 ttgcgatcgt cgcgatcggc ctgaccgtcg cgccgctgta cgaacgggtc gtgcggcgca
      121 cgctgtggat ttacggcgcg agctgcgtgg ttctgttctt catcccgaac cccgtgggcg
      181 gcaacatcgg ccggctcgct ggcatcatga tcggtcccgc cgcagcgttc gtgttgctgc
      241 gcgcgcaccg aaagactgca ctgttagttg ttgcggcacc gctgctgatc ttccaactgc
      301 tgccggtcat tggagcgatc tcgtcggctg ccggcgatcc gtcgacgaaa gcgcagtact
      361 accagggaat gttgagcttc ctcacccaaa atcagcagcc gctcggtcgc gtcgagattc
      421 cgtttactcg tgaccattgg gaagccaaat acgtcgccga gaaggtgccg ctggcacgcg
      481 gttgggaccg ccagatcgac ctctcgcgca acgctgtcct gtacgcaccg ctgaccgtgg
      541 cgacgtacca cacctggttg ttcgaaaacg cggtgcggtt cgtcgcgctg cccgacgctc
      601 cgcttgatca gggcggccag gcggagaagg ctttgttgcg gcatccgccg tcgtatctgc
      661 ggctggtgta cgcagacaac caatggcaca tctggtcggt cgttggagct gcaccgttgg
      721 cgagcggcgc cgcgacggtc accgcactca caccgagcag cttcacgatg aacgcgtcaa
      781 ccgggggcgt caccttcgtg cgtcttcggt ggtccgcgta ctggcacgtc gacaacgccg
      841 cccctgcgtg cgtggccccc gcgggcggcg gctggaccac cgtgctgagt tttgcgcctg
      901 ggacggtcaa gatcagcgct cgagccctgc ctggtgatac ccgatgcacc gacctgcagc
      961 tcgctcgttt cggtctcggc gatgccggcg cacccgaacc cacgtactga cgcccctaca
     1021 ttgcggcggc taccgccgcc cctacattgc ggcggctacc gccgcccatg actgcggccg
     1081 cgcgcccagc ccacgcccgg tctccggatt ccagtactcc gcgaaccccg cacgcaagca
     1141 cgcccgtttc gtgagcctgc caatctctgt cgcgagcgca ccgtcgccac attggcgtgc
     1201 cgagtatgcc gccaggtagt tcagctgcgg ccaggccggt ccgcgccagt actgcatggg
     1261 gttgtaggtc ggctgcgact tcggcacgaa gcgcggcccg tacggtgcgc cgaaccgggc
     1321 cgggtcggtg agttggcgca gtgccgccgc tgccttggcc gggtcgggtg tgcacatggc
     1381 cggcaacgcg gcgtccatcg tcgggatgcg cacgctgacg ccaccgccgg tgaacgtgac
     1441 gtccgaccac agtgcttcat cggcgtccca cgctgcgtcg tcaaaggtgg ccgcgagggc
     1501 tcggccgcgc gcgatccagg cggcatcgcc gatcagttcg cccagtgtcg ttgcggcgtc
     1561 ggctgcgatc gcattgaacg acgcaggcgc gacgacgaag tcggggttgt cgatcgcgtg
     1621 gcccgatgga ttgaacaccg tccgtttcag caatgcgcgg tcgaacgcgg tccagacccg
     1681 gcgattccac acgcttgcgc ccacccagct gtcccaccgt ggcgagtcgt cggtgcctgc
     1741 ttcccatgga tgcacgatga ccagcagtcc gtcgcgcagt cgatcgcgcc acagcgcggc
     1801 aagtgcacgc tgcgcgcaat cgagcaattc atcggacgcc gggaagccgg cgtccgctcc
     1861 tgctttcagc gcacgggcat agaccggcgg ctgggtgaac gacgacacgc ctttcagcgg
     1921 cccgcgggga taggtccgac ggccgtaccg catatgtgga aggaatccgt cgggcagctg
     1981 tccggtgaaa accgcctcga tttctcgcga ccctcgggcg tcgccgagtg cgaaccacgc
     2041 gatcgcgtga aagcacgaat cccacaacca caggtggggg tagctgcggc ggttcggctt
     2101 gcagtacccg gcagattcgt tccacgcgcg gtcgagcacg cgcgcggctc cggtacgcac
     2161 gatggcgtcg agccggtcga cgtccgtgat tccgtcacgg ccgtcgcggt ggtcgcggct
     2221 caaagcgacg acgggatggc cagcgacgct ttgtggtcgg gcgcggcgtc gcgcagcgct
     2281 gccgcggcgc ctgtctgctt cgcctggagc accgtcgtcg gaatcgttcc agtgtcagcg
     2341 ccgtgcgcaa cggcgtcgag gtacgccgtc cattcgtcga ggtaggcgag ctccgcggcc
     2401 ttcgccttgt gcagggcggt gtgccatggc agcaccgaag tcgaggcaac tgccgcccga
     2461 gtcgcgcgca ctttggcgac gccctggctt gccgactggt cgatgagctg ctcgagtccg
     2521 gcgcgtacgt tcgccggcgc ggagccgagc aacggcatcg tgtattgcct cgtcgattcg
     2581 agcgtcgcat ctgcgtgcgc cgcggttgct tgtccgttga tcgtcgcgcc gagcagtgtt
     2641 ttgaactcgt tgtgacgtga atcgcgatca agggccaccg cgccataggc gatcaggacg
     2701 accacacaca gcaccggaac gagaaaccgc ggcaggcgcc gagggctttc gcgtccgccc
     2761 gtgatcagaa catcggcgcg gtctgccgac tgatcgccgg tcatgtcaga ttgctgtcat
     2821 ctcaggccgc tgggtccagg attgccgcgg ccgcactgaa gatgtctgcg ccgaccgaac
     2881 tggtgccgcc gaacagccac gctgaggtcg cagacgattg cgccgtctcg aggtagccgg
     2941 caacggaact cgggagagtg ccgctgctcg gcaccaagac gatgggtccg tgattcatag
     3001 ccgccacgct gccacctgag agcgcgtcag ggaaggtcag gccgctagcg agcccgaccg
     3061 cagcaggtgt cgggaagaag gcctgggcca ccagcacgga cgtcgcgaag cggtcgctgc
     3121 cgacgaacgc ctttgccaac gggtcggcat gcgcagctgg gccgccgatg gcgtagcgct
     3181 gtgcgctcgg gtgagcggcc aggtaagccg cagtcgggcc ggactgtccc gagccggcgg
     3241 tcagcaacac gacgccgccc gtcgctgcgg cggccgaacc ggccgagagc gcatcgggga
     3301 agttcgtgcc gtccgcttcg aagacagtga tggggttgcc aagcttcgcg gcaacttgca
     3361 cggccgtggc aaaccggtcg gacccagcca cgcggacgac gggatggccg agcgcggcga
     3421 cggcgttcgc aacggccggt gagagcgcgg agataccgcc gagcatgtac accgttgcac
     3481 cgggcgcgag cactcgctgt atctcggcgg ccgtcgctgt cgacagtgac gcgctcgcgg
     3541 tcagcagcag cgggccgtgc aagccgacgg cgagcggggt gccggcaagt gcatcggcga
     3601 aggtgtcgga gcgcgccaac acgaccgtgg ccgctgaatg cgctgttggg aagcccgttt
     3661 gcgatgctgc aacgctcgtg gcgatgcgat cggcacccgc gacgcgtccc agcgtgagtg
     3721 cctgcgcagc agatccggca ccgccgccgc cgccgctggt gccaccgccg ctggtgccac
     3781 cgccgccacc actaccgacg gagacggacg gcgaggcgag ggtggtcaag a
//
LOCUS       DATCBJ010000324         2139 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_311683, whole genome shotgun sequence.
ACCESSION   DATCBJ010000324 DATCBJ010000000
VERSION     DATCBJ010000324.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2139)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2139)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2139
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_311683"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(211..>2139)
                     /locus_tag="VII50_06460"
     CDS             complement(211..>2139)
                     /locus_tag="VII50_06460"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014525059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0032549 - ribonucleoside binding [Evidence
                     IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HEY5050524.1"
                     /translation="EEDRFVIAQANAPLKDDGHFAEDRVLVRRKGGEVDFVTPAAVDY
                     MDVSPRQMVSVATAMIPFLEHDDANRALMGSNMQRQSVPLLKSEAPLVGTGMEFRAAV
                     DAGDVVTSAKDGVVEEVSSDAITVMNDDGTRTTYRMAKFRRSNQGTCINQRPIVSEGQ
                     RLQAGAAIADGPCTDQGEMALGRNLLVAFMPWEGHNYEDAIILSQRLVQDDVLSSIHI
                     EEHEVDARDTKLGPEEITRDIPNVSEEVLADLDERGIIRVGADVVPGDVLVGKVTPKG
                     ETELTPEERLLRAIFGEKAREVRDTSLKVPHGEKGKVIDVKVFTRDDAELPPGVNELV
                     RVYVAQKRKITDGDKLAGRHGNKGVISKILPEEDMPFLADGTPVDIVLNPLGVPGRMN
                     VGQVLETHLGWAAKSGWKVESTGAKDGWQQRLADIGALEAPPNTLVATPVFDGAREDE
                     ITGLLGSTLPNADGQRPVGENGKARLYDGRSGELYPEAVSVGYIYILKLHHLVDDKLH
                     ARSTGPYSMITQQPLGGKAQFGGQRFGEMEVWALEAYGAAYALQELLTIKSDDVLGRV
                     KVYEAIVKGENIPEPGIPESFKVLIKEMQSLCLNVEVLSSDGMTLEMRDTDDEVFRAA
                     EELGIDLSRREPSSVEEV"
BASE COUNT          353 a          706 c          661 g          419 t
ORIGIN      
        1 caggacgaag cgtgcggtag ttgatcgtct ccggcttctt gacctcgcca tgagaccact
       61 aacgaatgtc gtcagcggtg gcgaggccga tacgcaactc atcgaagaag ttgacgtcaa
      121 gcacaggttg tcttctctct cctggtaatg cgtcgatctt gctgttggtc tgtgtagcgt
      181 ctaaaccggc gggtcagcgc cggggcggcc tcagacctct tcgacgctgc tcggctcgcg
      241 ccgggacagg tcgataccga gctcttccgc ggcgcggaag acctcatcgt cggtgtcgcg
      301 catctccaac gtcatgccgt cgctggaaag cacctcgacg ttgaggcaca gcgactgcat
      361 ttccttgatg agcaccttga acgactcggg aatgccgggt tcggggatgt tctcgccctt
      421 aacgatggcc tcgtagacct tgaccctgcc aaggacgtcg tcggacttga tcgtgagcaa
      481 ctcctgcagc gcgtaagccg cgccgtaggc ctcaagcgcc cagacctcca tctcaccaaa
      541 ccgctggcca ccgaactgcg ccttaccacc caacggctgc tgggtaatca tcgagtacgg
      601 gccggtcgac cgcgcgtgga gcttgtcgtc gacgaggtgg tgcagtttca ggatgtagat
      661 gtatccgacg ctgaccgcct ccgggtagag ctcaccggag cgaccgtcgt acagccgcgc
      721 cttgccgttt tccccgaccg gacgctgtcc gtcggcgttc ggcagggtcg aaccgagcag
      781 gccggtgatc tcgtcctcgc gggcaccgtc gaagaccggc gtcgcgacga gcgtgttcgg
      841 cggagcctcg agcgcgccga tgtcggcgag ccgctgctgc cagccatcct tcgcgccggt
      901 cgactcgacc ttccagccac tcttcgcagc ccagccaagg tgcgtttcga gaacctggcc
      961 gacgttcatg cggccaggca cacccagcgg gttgagaacg atgtccacag gtgtgccatc
     1021 ggcgaggaac ggcatgtctt cttcgggcag gatcttggag atgacgccct tgttcccgtg
     1081 ccggccggcc agcttgtcgc cgtccgtgat cttgcgcttc tgggcgacgt atacccgcac
     1141 cagttcgttg acgcccggcg gcagctcagc gtcgtcacgg gtgaacacct tgacgtcgat
     1201 gaccttgccc ttttcaccgt gcggcacctt cagcgaggtg tcgcgcacct cgcgcgcctt
     1261 ctcaccgaag atcgctcgca gcaatcgctc ttctggcgtc agctcggtct cgcccttggg
     1321 cgtgaccttg ccgacaagaa cgtcacccgg gacgacgtcg gcgccgacgc ggatgatgcc
     1381 gcgctcgtcg aggtcggcca gcacttcctc gctgacgttc gggatgtcgc gggtgatctc
     1441 ctccggcccg agcttggtgt cgcgggcatc gacttcgtgc tcctcgatgt ggatcgatga
     1501 gagcacgtcg tcttgcacga gacgctgcga caagatgatc gcgtcttcgt agttgtgacc
     1561 ttcccacggc atgaacgcca cgagcaggtt gcgacccaac gccatttcac cctggtcggt
     1621 gcacggaccg tcggcgatcg ccgcgccggc ctgcagtcgc tggccctcac tgacgatcgg
     1681 gcgctggttg atgcaggtgc cctggttgga acggcggaac ttcgccatcc ggtacgtcgt
     1741 gcgagtgccg tcgtcgttca tgacggtgat cgcgtcggag gaaacctctt cgacgacacc
     1801 gtccttcgcg gacgtgacaa cgtcgccggc gtcgacagcg gcacgaaact ccatgccggt
     1861 gccgaccaat ggcgcctcag acttcaacag cggcaccgac tggcgctgca tgttggagcc
     1921 catcagtgcc cggttcgcat cgtcgtgctc gaggaacggg atcatcgcgg tcgcgaccga
     1981 caccatctgg cgcggcgaga cgtccatgta gtcgactgct gcgggcgtga cgaagtcgac
     2041 ctcgccaccc ttacggcgaa ccaacacccg gtcttcggcg aagtgaccgt cgtccttcaa
     2101 cggcgcgttc gcctgagcga tgacgaaacg atcctcttc
//
LOCUS       DATCBJ010000325         2414 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_40113, whole genome shotgun sequence.
ACCESSION   DATCBJ010000325 DATCBJ010000000
VERSION     DATCBJ010000325.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2414)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2414)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2414
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_40113"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..551
                     /locus_tag="VII50_06465"
     CDS             <1..551
                     /locus_tag="VII50_06465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009072508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HhH-GPD-type base excision DNA repair protein"
                     /protein_id="HEY5050525.1"
                     /translation="DADALLDRDPLALLVGMLLDQQFPMERAFAGPYLLAQRMGVDHL
                     NAYDMAEYDPEKFATLFVGPPAIHRFPGAMAGRVQQLAHLLVADYDGSAEAVWKGAKS
                     GAELHGRIATLPGFGQQKARIFVALLGKQRGVRPRGWREAAGAYGEDGSFRSVADVTS
                     PESLQKVRDHKKELKAAARAGA"
     gene            783..1112
                     /locus_tag="VII50_06470"
     CDS             783..1112
                     /locus_tag="VII50_06470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5050526.1"
                     /translation="MSNIEEIGLELNQRAVQVEMAGAQLVQAAAEAIWTSIAADAFRT
                     RVDHRRQQFGAVADLLRGAAQTVFRYADDVEAEQARLRRLEQAAEHAVIRGVVGVGGV
                     VIGGIHL"
     gene            1109..>2414
                     /locus_tag="VII50_06475"
     CDS             1109..>2414
                     /locus_tag="VII50_06475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5050527.1"
                     /translation="MSGAAEIVDYEELARMSSVWGEAAASMARTGFHVAALAGSSELL
                     AGAMFDPMGAARAEAGILRAAVGPRGLAALGAGLEVDSVLLKAVIAKEQYVDDLPLHQ
                     VAALERWLVALPLELTLDPSRALRDGTRDTAALANAITGYAAPHTQALLELFAPSWRF
                     RLDVAQRRPMAVDPVFGLPLALMVPGNERTGGSVSISRYAPLWGRRPPPSVGSMFDRV
                     GDLEDQPEASIAVQRVAGTDGVARYVVVLSGMRSMTNTSDPEDLLGSGAAVVATTTNY
                     TTCVREAIDAALVPRGAEVLLVGHSEGGIVAMDLAGDPAFNGGRVRVMQVVAAGSPIS
                     SKPVAGGSGTRVLSIENVNDIVPHLDAADPPASHQSVERLTYRFADDEHNIVASHDVS
                     LYARQAAGLAGSPNPLMIDVQAGLRPFMGGSATTTVFTLHDRV"
BASE COUNT          374 a          770 c          849 g          421 t
ORIGIN      
        1 cggacgccga cgcgttgctc gaccgcgatc cgctggctct gctcgttggc atgctgctcg
       61 accagcagtt ccctatggag cgggccttcg ccggtccgta cctgctcgcg caacggatgg
      121 gcgtcgatca cctcaacgcg tacgacatgg ccgagtacga ccccgaaaag ttcgccacgc
      181 tgtttgtcgg gccaccggca atccaccgtt ttccgggggc catggctggc cgcgtgcagc
      241 agctcgctca tctgttggtt gcggactacg acggcagcgc tgaggcggta tggaagggcg
      301 cgaaatctgg tgcggagttg cacggccgaa tagcgacgct gcccggtttc ggtcagcaaa
      361 aggcgcggat tttcgttgct ctgctaggga aacagcgtgg cgtgcggcca cgcggttggc
      421 gcgaggcggc gggcgcgtac ggcgaagacg gcagcttccg gtcggtcgcc gacgtcacca
      481 gcccggagtc gttgcagaag gttcgtgatc acaagaagga gctgaaggcg gcggcgcgcg
      541 caggcgcgta gttacggctc ttgttcggcg tcgacatcga cgcgcaacgg gtagacgaag
      601 tcggcaggca gccacggcat ggcacgaggg tagggctcgc cgtcctcccg ggaagcgttg
      661 actctccaca gccgccagcg ctctccactg aagcttgcgc gcccaaccgt agtgcgaccc
      721 gccactcgta ggttcgtcga gacgggcgcg acgtcggcgc ggttacggcg aatggtggtg
      781 cgatgtcgaa catcgaggag atcgggctag agctcaacca gcgcgcagta caggttgaga
      841 tggccggcgc acaattggtg caggctgctg ctgaggcaat ctggacgtcg atcgccgccg
      901 atgcatttcg cactcgggtc gatcaccggc ggcagcagtt cggcgcagtc gccgatctcc
      961 tgcggggtgc cgcgcagacc gtattccggt atgccgacga cgttgaagcg gagcaggcgc
     1021 gattgcgcag actcgagcaa gccgctgagc atgcggtgat ccgcggtgtg gtcggggtgg
     1081 gcggcgttgt catcggcggc atccacctgt gagcggtgcg gccgagatcg tcgactacga
     1141 agagctcgct cgcatgtcat cggtatgggg cgaggctgcg gcgtcgatgg cgcgcaccgg
     1201 gttccacgtc gccgcgctcg ctggctcgtc cgagctgctg gcgggcgcga tgttcgaccc
     1261 gatgggtgcc gctcgagccg aagcgggcat tctgcgcgcc gcagtcgggc cgcgcggcct
     1321 ggctgccctg ggcgccggcc tggaggtcga ctccgtactc ctcaaagccg ttatcgccaa
     1381 ggagcagtac gtcgacgacc tgccgttgca ccaggtcgcg gcgctcgagc gatggctcgt
     1441 cgcgttgccg ctggagttga cgctcgatcc cagccgcgca ctgcgtgacg gcactcgcga
     1501 taccgcggcg ctcgccaacg ccatcaccgg ctacgcggct ccgcacacgc aggcactcct
     1561 cgaattgttc gcgccctcct ggcgatttcg tctcgacgtc gcgcagcgtc ggccgatggc
     1621 cgttgatccc gtgttcggct tgccgcttgc gctgatggtt cctggtaacg agcgaacagg
     1681 cggcagtgtc tcgatatcga gatacgcgcc tttgtggggc cgccggccgc cgccgtccgt
     1741 cggctcgatg ttcgatcggg tcggcgatct cgaggatcag ccggaggctt cgatcgcggt
     1801 gcagcgggtg gcgggcacag acggggttgc gcgctatgtc gtcgtcctct cgggcatgcg
     1861 aagcatgacc aacacctcag accccgagga tctgcttggg tctggcgcgg cagtcgtcgc
     1921 cacgacgacg aactacacca cctgcgtgcg cgaggcgatt gacgccgcac tcgttccgcg
     1981 cggcgccgag gtgttgctcg tggggcacag tgagggtggc atcgtcgcga tggatctggc
     2041 cggcgatcca gcgttcaacg gcggccgggt ccgggtgatg caggtggtgg cagcgggatc
     2101 gccgatcagc tccaagccag ttgccggggg cagcggcacc cgggtcctga gcatcgaaaa
     2161 cgtgaacgac atcgtgcccc acctcgatgc cgccgacccg ccggcgtccc atcagagcgt
     2221 tgagcggttg acctatcgat tcgccgacga cgagcacaac atcgtggcca gccacgacgt
     2281 gtcgctctac gcccgccagg cagcgggcct ggctggctcc ccgaacccgc tcatgatcga
     2341 cgtccaggca ggtttacgcc cgtttatggg cggttcggcg acgacgacgg tgttcaccct
     2401 gcatgatcgc gtga
//
LOCUS       DATCBJ010000326         2715 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_515489, whole genome shotgun sequence.
ACCESSION   DATCBJ010000326 DATCBJ010000000
VERSION     DATCBJ010000326.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2715)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2715)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2715
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_515489"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..784
                     /locus_tag="VII50_06480"
     CDS             <1..784
                     /locus_tag="VII50_06480"
                     /inference="COORDINATES: protein motif:HMM:NF024869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="XdhC/CoxI family protein"
                     /protein_id="HEY5050528.1"
                     /translation="GAAVGAKLLYRPGEPLIGTLGTAELDVVAARDAAGELAAGSSRL
                     RHYGGRGEIGRDEVSVFIESFVAPARMIIFGAVDFTAALVRMSRVMGFRVTVCDARPA
                     FATKARFPLADEVVVDWPDRYLRRLREPLTSRDAVCVLTHDSKFDVPAIVAALDTAVG
                     YLGALGSRRTHIDRVIRLREAGVADDGLARLHAPIGLDLGARTPEETAIAICAEIISV
                     RTGRTDIKPLTDSERPIHDPLLGTPTAGAASVGRDEGHLVRS"
     gene            919..1752
                     /locus_tag="VII50_06485"
     CDS             919..1752
                     /locus_tag="VII50_06485"
                     /inference="COORDINATES: protein motif:HMM:NF021214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase assembly protein"
                     /protein_id="HEY5050529.1"
                     /translation="MSVDWARVVTNWQTDAVSVTGIVLELAVVAAYCYGVARVRANGR
                     GWPTYRLVAFVLGVGSLALMLQSGFAAYDALLWVHMTQHLVLMMVAAPLLALGAPVRL
                     CFAAGSASVRRFVAELLHDPSMRLITGTTAAVLLPLDYFGSMAVYLLTPLYRLSELNQ
                     GFHEFVHVYFLACGLMFWVPMLGQDPVAWRPTPRLKLALVTAGVPIYMAISAAMFAEG
                     SFITPEHSLADIHRGVVAMLVGGVVLTLAGDALVLWCERRRTTAIRERASNRRLLVST
                     S"
     gene            1820..>2715
                     /gene="moaA"
                     /locus_tag="VII50_06490"
     CDS             1820..>2715
                     /gene="moaA"
                     /locus_tag="VII50_06490"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: protein motif:HMM:TIGR02666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HEY5050530.1"
                     /translation="MTDRCNFRCIYCMPRESFGASHAFLPRAEILSYEEFARLVSVFA
                     QLGVSKVRLTGGEPLLRKDLETLVSMVAATPGIADVALTTNGSLLASRAAALRDAGLD
                     RVTVSLDSIDAEMFAAMADTRLSLSTVLDGIAAAADVGLTPVKLNAVVRRGRNDNGIV
                     ELAEFARAHGHVLRFIEYMDVGDTNNWVLDDVVPSAEVVARISAVFPVEPMPSTRLGE
                     VAKRWRYVDGAGEIGVISSVTQPFCTDCTRARVTSTGELFTCLFGSGKLDLRAMLRRG
                     AVDDELRRAISAVWSGRRDQYS"
BASE COUNT          399 a          847 c          940 g          529 t
ORIGIN      
        1 gggagcggca gtcggcgcga agctgctgta ccgcccaggc gaaccgttga tcggcacgct
       61 ggggacggcg gaacttgacg ttgtcgcggc ccgggatgcc gcgggggagc tcgctgctgg
      121 gtctagccgg ttgcggcact acggagggcg cggggagatc ggccgcgatg aggtctcggt
      181 cttcatcgag tcgtttgtgg cgcccgcgcg catgatcatt ttcggggccg tcgatttcac
      241 tgccgcgctc gtgcgcatgt cgcgggtgat gggatttcgg gtgaccgtgt gtgacgcgcg
      301 tcccgcgttc gccacgaagg cgcgcttccc gctggccgac gaggtcgttg ttgactggcc
      361 cgatcgctat ctgcggagat tgcgcgagcc gctgacttcg cgcgatgcgg tgtgtgtgct
      421 gacgcacgat tcgaagttcg acgttcctgc gatcgtcgcc gcgctcgata ccgcggtcgg
      481 ctatctcggg gcgctcggga gtcgacgcac ccatatcgac cgagtgatcc ggcttcgtga
      541 ggcgggtgtc gctgatgacg gattggcccg gttgcacgca ccgatcgggc tcgacctcgg
      601 tgctcgtaca ccggaagaaa ccgcgatagc gatctgtgcg gagatcatct ccgtgcgcac
      661 cggtcgtacc gacatcaagc cgctgaccga cagcgagcgg ccgatccacg acccacttct
      721 tgggacgccg acggcgggcg ccgcgtcggt cggacgagat gaggggcacc ttgtcaggag
      781 ctagaccacc gagctcatcc gcgcgcggat cgggagggcg atcgtgtcct gctgcctgat
      841 cgtgatgatt gtcggccgcc ggctcgcgcg gcgagcggtt gtggtcggcg cccgattccg
      901 gcgccaaacg agtgccgtgt gagcgtcgac tgggcgcgtg tcgtcacgaa ctggcaaacc
      961 gacgcggtgt cggtgaccgg aatcgtcctc gagctcgcag tcgtagcggc gtactgctat
     1021 ggcgtcgcaa gggtacgggc caacgggcgc ggctggccga cgtatcgctt ggtggccttc
     1081 gtgctcggcg ttggttcact ggcgctcatg ctccaatccg gctttgcggc ctacgacgcc
     1141 ctgctgtggg tgcacatgac ccagcacttg gtgctgatga tggtcgcggc gccgctcctc
     1201 gcattaggtg caccagtccg gctctgcttc gccgctggtt cggcatcggt gcggcgtttc
     1261 gtcgccgagc tgctgcacga tccgagcatg cggctgatca ccggcaccac agcggcggtg
     1321 ctcctcccgc tcgactactt cgggtcgatg gcggtttacc tgctcacgcc gctctaccgg
     1381 ctgtcggagc tgaaccaggg cttccacgag ttcgtgcacg tgtactttct ggcctgtggg
     1441 ctcatgttct gggtgccgat gctcggccag gatccggtcg cctggcggcc aacgccccgg
     1501 ctcaagctcg cactcgtcac ggcaggggtg ccgatctaca tggcgatctc ggccgcgatg
     1561 ttcgccgaag gcagcttcat cacccccgag cacagcctgg ccgatatcca tcgcggtgtc
     1621 gtggcgatgc tggttggcgg tgtcgtcttg acacttgcgg gcgacgcgct cgtgttgtgg
     1681 tgtgaacgcc gacggaccac cgcgattcgc gagcgcgcga gcaaccgacg cttgctggtc
     1741 tcgacgtcgt gagccggccg tcgtgaccac tggcccgttg gaccagttcg gccgaccggt
     1801 gcgcgatctg cgactttcgg tgactgaccg gtgcaacttc cggtgcattt attgcatgcc
     1861 aagggaatcg ttcggagcga gccacgcgtt tctgcctcgt gcggaaatcc ttagctacga
     1921 ggaattcgct cgcctggtgt cggtttttgc ccagctcggc gtcagcaagg tgcggctcac
     1981 cggtggtgag ccgctactac gaaaagatct cgagacgctc gtctcgatgg tggccgcgac
     2041 gccggggatc gccgacgtcg cgctgacgac gaacggctcg ctgctcgcga gcagggcggc
     2101 cgccttgcgc gacgccggac tcgaccgagt cacggtcagc ctcgacagca tcgacgccga
     2161 gatgttcgcg gcgatggccg acacgcgact gtcgttgagc actgtgctcg acggaatcgc
     2221 ggcagcggca gatgtcggcc tcaccccggt caagctcaat gcggtcgtgc gacgaggccg
     2281 caacgacaat ggcattgtcg aactcgcgga attcgctcga gcgcatggac atgtgcttcg
     2341 gttcatcgaa tacatggacg tcggtgacac caataattgg gtgctggacg acgtggttcc
     2401 gtcggcggag gtcgtcgccc gcatcagtgc cgtcttcccc gtcgagccga tgccgtcgac
     2461 ccggcttggc gaggtggcga agcgctggcg ctacgtcgac ggcgcgggcg aaatcggcgt
     2521 gatctcctcc gtgacacaac cgttctgcac cgactgcacg cgagcccgag tgacatctac
     2581 cggcgagttg ttcacctgct tgttcggatc agggaagctc gacctacggg cgatgctgcg
     2641 ccgaggcgcc gttgacgacg aactgcgtcg ggcgatctct gcagtgtggt cggggaggcg
     2701 cgatcagtac tcgca
//
LOCUS       DATCBJ010000327         2609 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_491196, whole genome shotgun sequence.
ACCESSION   DATCBJ010000327 DATCBJ010000000
VERSION     DATCBJ010000327.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2609)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2609)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2609
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_491196"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..515
                     /gene="fusA"
                     /locus_tag="VII50_06495"
     CDS             <1..515
                     /gene="fusA"
                     /locus_tag="VII50_06495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719140.1"
                     /note="EF-G; promotes GTP-dependent translocation of the
                     ribosome during translation; many organisms have multiple
                     copies of this gene; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HEY5050531.1"
                     /translation="IKGGSVPSEFIPAVEKGCEEALESGVKAGYPMVDIRVTLTDGKY
                     HDTDSSEIAFKVAGSLAFKEAARRAKPVLLEPIFGVEVVTPEEFLGEVIGDLSRRRGR
                     IEGQERRGNALTVTARVPLAEMFGYATDLRSNTQGRANYTMQFEAYEEVPPNIAEKVV
                     ESRGGESKAA"
     gene            612..1796
                     /gene="tuf"
                     /locus_tag="VII50_06500"
     CDS             612..1796
                     /gene="tuf"
                     /locus_tag="VII50_06500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005055596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HEY5050532.1"
                     /translation="MGKQKFERDKPHVNVGTIGHIDHGKTTLTAAITNVLAEKMGGEA
                     KSFEEIDNAPEERERGITIATSHVEYQTENRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVSAADGPMPQTREHILLARQVGVPYIVVFLNKADMVDDEELLELVEVEVRELLSY
                     YDFPGDDIPFVIGSALKALEGDEPSREKILELAAALDSYIPEPVRELDKPFLMPVEDV
                     FSITGRGTVATGRIEQGIIKTGETIEIVGIKATVNTVVTGVEMFRKILDQGQAGDNVG
                     CLLRGTKREEIERGQVLCKPGSITPHTHFKSEVYVLKKEEGGRHTPFFSGYRPQFYFR
                     TTDVTGVANLPEGVEMVMPGDNVQMEIELIQPIAMDQGLRFAIREGGRTVGSGVVTEV
                     IK"
     gene            1987..2310
                     /gene="rpsJ"
                     /locus_tag="VII50_06505"
     CDS             1987..2310
                     /gene="rpsJ"
                     /locus_tag="VII50_06505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007047297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HEY5050533.1"
                     /translation="MASAQNKIRIRLKAYDTQAIETAAKEIVDTATRTGATVSGPVPL
                     PTEKNVYCVIRSPFKDKDSREHFEIRTHKRLIDIHHPTPKTVDSLQRLDHLPAGVDIE
                     IRLAS"
     gene            2310..>2609
                     /gene="rplC"
                     /locus_tag="VII50_06510"
     CDS             2310..>2609
                     /gene="rplC"
                     /locus_tag="VII50_06510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="binds directly near the 3' end of the 23S rRNA,
                     where it nucleates assembly of the 50S subunit; essential
                     for peptidyltransferase activity; mutations in this gene
                     confer resistance to tiamulin; Derived by automated
                     computational analysis using gene prediction method:
                     GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HEY5050534.1"
                     /translation="MAAILARKLGMTQVFGEDGAVARVTVLAAGPCHVTAIRTSERDG
                     YDAVQLAFGSVREKALTKPELGHLAKADAPPLRHLVEFRNEAGGLAVGETVTVSAF"
BASE COUNT          512 a          854 c          860 g          383 t
ORIGIN      
        1 agatcaaggg cgggtcggtg cccagcgagt tcatcccggc ggtcgagaag ggctgcgagg
       61 aggcgctcga gagtggtgtc aaggccggct acccgatggt cgacatccgg gtcaccctga
      121 ccgacggcaa gtaccacgac accgactcgt cggagatcgc cttcaaggtc gccggctcgc
      181 tcgcgttcaa ggaagcggcg cgccgcgcca agccggtatt gctcgagccg atcttcggcg
      241 tagaggtcgt cacgcccgag gagttcctcg gcgaggtgat cggcgacctc tcgcgccggc
      301 gcggtcgaat cgagggccag gagcgtcgcg gcaacgcgct cacggtgacg gctcgcgttc
      361 cgctcgcgga gatgttcggc tatgccaccg acctgcggtc caacacccag ggtcgagcga
      421 actacacgat gcagttcgag gcctacgagg aagttccgcc gaacatcgct gagaaggttg
      481 tcgagagccg cggaggcgag tcgaaggcgg cctagcagcg aggacaccgc gcagagtgcg
      541 gtatttctga gagtattccc gggttcgcct ccagtggcgg gccccaaacg tcgatcacag
      601 gagcaacaca agtgggcaag cagaagttcg agcgcgataa gccacacgtc aatgtcggaa
      661 cgatcggtca catcgatcac ggcaagacga cgctgaccgc tgccatcacc aacgtgcttg
      721 ccgagaagat gggcggtgag gcgaagagct tcgaggagat cgacaacgct cccgaggagc
      781 gtgagcgcgg catcacgatc gcgacctcac acgtcgaata ccagacggag aaccgccact
      841 acgctcacgt cgactgcccg ggtcacgccg actatgtgaa gaacatgatc accggcgccg
      901 cgcagatgga cggggcgatc ctcgtcgtct cagccgctga cggcccgatg ccgcagacgc
      961 gcgagcacat cctgctcgca cgccaggtcg gcgtccccta catcgtcgtg ttcctcaaca
     1021 aggccgacat ggtcgacgac gaggagctgc tcgagttggt cgaggtcgag gtgcgcgagc
     1081 tgctctccta ctacgacttc ccgggcgacg acatcccgtt cgtaatcggc tcggcgctca
     1141 aggcgctcga gggcgacgag ccctcccgcg agaagatcct cgagctcgcc gcggcgctgg
     1201 attcctacat cccggagccg gtgcgcgaac tcgacaagcc gttcctgatg ccggtcgagg
     1261 atgtcttctc gatcaccggc cgcggcacgg tggccaccgg ccgcatcgag cagggcatca
     1321 tcaagaccgg cgagacgatc gagatcgtcg gcatcaaggc caccgtgaac accgtcgtca
     1381 ccggcgtcga gatgttccgc aagatcctcg accagggcca ggcgggcgac aacgtcggct
     1441 gcctgctgcg cggcaccaag cgcgaggaga tcgagcgcgg ccaggtgctg tgcaagccgg
     1501 ggtcgatcac gccacacacg catttcaaga gcgaggtcta cgtgctgaag aaggaggagg
     1561 gtggccgcca cacgccgttc ttctccgggt accggcctca gttctacttc cgcaccaccg
     1621 acgtcaccgg cgtggcaaac ctgcccgagg gtgtcgagat ggtgatgccg ggagacaacg
     1681 tccagatgga gatcgagttg atccagccga tcgcgatgga ccagggtcta cggttcgcga
     1741 tccgcgaagg cggccgcacg gtcggctccg gtgtcgtgac cgaggtcatc aagtaaggca
     1801 ccaaagcggg tagcgctcgg tccgctgcct gccccccaga cagatacgac tattcacacc
     1861 ggacggttgc tcagaccggt gaggcgggct ccagtggctc gagcccgcct gtttgcgccc
     1921 gccgcgagcg aatcgcgacg atgacatatc agcagcacga caggatgaaa tgagaacgct
     1981 ggcctgatgg cctccgccca gaacaagatc agaatcagac tcaaggccta cgacacccag
     2041 gcgatcgaga cggccgccaa ggagatcgtc gacacggcga cgcgcaccgg cgcgaccgtg
     2101 tccggccccg tgccgctgcc gaccgagaag aacgtgtact gcgtgatccg ctcgccgttc
     2161 aaggacaagg attcgcgcga gcacttcgag atccgcaccc acaagcggct gatcgacatc
     2221 caccatccga cgccgaagac cgtcgactcg ctccagcgac tcgaccacct gccggccggc
     2281 gtcgacatcg agatccggct ggccagctga tggcagcgat cctcgcacgt aagctcggca
     2341 tgacgcaggt cttcggcgag gacggtgccg tcgcccgtgt caccgtgctg gcagcggggc
     2401 cctgccacgt caccgcgatc cgcacgagtg agcgcgacgg ctacgacgcg gtgcagctcg
     2461 cgttcggcag cgtccgcgag aaggcgctga cgaagcccga actcggccat ctggcgaagg
     2521 ccgacgcgcc gccgctgcgc cacctcgtgg agtttcgcaa cgaggccggc ggcctggcgg
     2581 tcggcgagac ggtgaccgtg tcggcgttc
//
LOCUS       DATCBJ010000328         4452 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_363998, whole genome shotgun sequence.
ACCESSION   DATCBJ010000328 DATCBJ010000000
VERSION     DATCBJ010000328.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 4452)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4452)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4452
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_363998"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            37..393
                     /locus_tag="VII50_06515"
     CDS             37..393
                     /locus_tag="VII50_06515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5050535.1"
                     /translation="MTDEEPISYMALQPGTDVCTADGTTIGTVEHVLQEPDLDLFDGI
                     VVAVPRGIRFVDRDQITSITTAKVTTSLSVADAAQLPEPEGNPVYHVNAMQDVGPALT
                     ARLGRMFRREHWIKDK"
     gene            complement(421..1224)
                     /locus_tag="VII50_06520"
     CDS             complement(421..1224)
                     /locus_tag="VII50_06520"
                     /inference="COORDINATES: protein motif:HMM:NF012455.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin"
                     /protein_id="HEY5050536.1"
                     /translation="MEQTAGQAARGGVHPVEWACEAAATALLLIAGLSAVCFDFGPSS
                     PLRHFGTSPRLLVTGLLFAGAGSLLAISPLGRRSGAHLNPVVTIAFWTQGKVHPHDVA
                     GYIVSQLVGAVTGTAVVALLWRSQASAVHLGATVPGRGLNGIEAVLVEATMTGVLILV
                     ILLMTSGPAARWTPLVLWPLIAGLVWQGAPFTGTSMNPARSFGPALLAPRLQPYWIYV
                     VGPLAGGALAVAAFGLWPKRRVLTAKLFHDPTYPTTFASSLPVAKRSPE"
     gene            complement(1281..2345)
                     /locus_tag="VII50_06525"
     CDS             complement(1281..2345)
                     /locus_tag="VII50_06525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009081512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HEY5050537.1"
                     /translation="MGEEVPDPIGAPDPIDALRQIAFLLERAREPTYRVKAFRTAAAV
                     LAALPEGELEQRAKRGTLTDLAGVGAVTATVAREALAGTVPAYLAKLLPAGDEPLVEG
                     GESLRATLRGDLHTHSDWSDGGSPIEEMALAARDLASGPHDYIALTDHSPRLTVASGL
                     TAERLRDQLRVVAELNEKLAPFRILTGIECDILDDGSLDQEPELLDQLDIVVASVHSK
                     LKMDRIAMTRRMLAAVRNPLVDVLGHCTGRLLIGRDGGKSRLESQFDAEKVFAACKQH
                     DVAVEINSRPERLDPPRRLLSMAVEIGCRFSVDTDAHAPGQLDWQIYGCARAQECGVD
                     ESKVVNTMDADELLKWAQRS"
     gene            complement(2350..3552)
                     /locus_tag="VII50_06530"
     CDS             complement(2350..3552)
                     /locus_tag="VII50_06530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FIST N-terminal domain-containing protein"
                     /protein_id="HEY5050538.1"
                     /translation="MARFGDGLAIDADLVRAAEAAAIAARGPLGGLSPDLACVFVCGS
                     EPDQVGEALQRAASVLRARTVIGCSASGVIGAAQGIEATSAVSVWAAVLPGVRVRAFH
                     LEVIRTEGGMAILGLPESEDADEVAVVVADPYSFPADGFVEQANTALALPLVGGLAAG
                     NRGPGSTRLLIDGRVVDRGAVGVVLGGPVGAQAMVSQGCRPVGPAMTVTAAEGNLLFE
                     LAGIPAIQKLEQVIGSLSSIDQALASGGLQIGIAMDEYVEEHERGDFLVRGILGIDKA
                     RGAIAIGDIVPVGRTVRFQVRDAASADDDLRAMLVQLKHEFSAVEGALLFSCNGRGSN
                     LFTSADHDVLALRQGLATTGIAGFFGSGEIGPVGNRNHLHGFSASVLVFGSPRPAGHI
                     DHDNAVPR"
     gene            complement(3722..4138)
                     /locus_tag="VII50_06535"
     CDS             complement(3722..4138)
                     /locus_tag="VII50_06535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5050539.1"
                     /translation="MPTDDLARITRPFGDVGVDFEPPGAGTTTLHYRLEVIVKRPPID
                     PTLSPPSDAYGAAVSALVDSYRKLAVGTEPHVARVTLDRFRQGPLVLPAAVLNALPKG
                     GSYTLYLVSGGDMSHCPFDFDSSQIQGDFPIADVIT"
     gene            4227..>4452
                     /locus_tag="VII50_06540"
     CDS             4227..>4452
                     /locus_tag="VII50_06540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5050540.1"
                     /translation="MAEASADAEAMVATATLVLGGAMLTGAVLAGVVLAGAVLAAGGL
                     AAAVALAAGVVAADVVAATPARDEHPDSNPT"
BASE COUNT          749 a         1524 c         1483 g          696 t
ORIGIN      
        1 tcgccggcga ctgacccgga ttgacaggag accactatga ccgacgaaga gccaatctcg
       61 tatatggcac tccagcccgg cactgacgtg tgcacggccg acggcaccac catcggcacg
      121 gtcgaacacg tcttgcagga accagacctc gacctgttcg acggcatcgt ggtcgcggta
      181 ccgcgcggaa ttcgcttcgt cgaccgggac cagatcactt cgatcaccac cgcgaaagtg
      241 acgacgagcc tcagtgtcgc ggacgctgcg cagttgcccg agccggaggg caatccggtc
      301 taccacgtca acgccatgca ggacgtcggg ccggcgctga ccgcacggct gggccgcatg
      361 ttccgccgcg agcactggat caaggacaag taggtgcgct cgatcgcttc gccaccggca
      421 ctactccggc gatcgcttcg cgaccggcag tgaactcgcg aacgtcgtgg ggtacgtcgg
      481 atcgtgaaag agcttggccg tgagcactcg acgtttcggc cacaacccga acgccgcaac
      541 ggcgagcgca ccgccggcga gcggaccgac gacatagatc cagtacggct ggagccgcgg
      601 tgcgagcagc gcggggccga agctgcgcgc cgggttcatg ctcgttccgg tgaacggcgc
      661 accttgccag accagccccg cgatcagcgg ccacaagacg agcggcgtcc atcgtgcggc
      721 cgggccgctg gtcatcaaca gaatcaccaa gatcagcacc ccggtcatcg tcgcttcgac
      781 gagcaccgct tcgatgccgt tcagccctct gcccggaacg gtcgcgccga ggtgcacggc
      841 actggcctgc gacctccata gcagggccac cacagcggta cccgtgacgg cgcctacgag
      901 ttggctcacg atatagccgg cgacgtcgtg tggatggact ttgccttgcg tccagaaggc
      961 gatcgtgacg acggggttca ggtgggcgcc gctacgcctc ccgagtgggc tgatcgcgag
     1021 caggctgccg gcgccggcga acaagaggcc ggtgaccaac agccgcggcg acgtgccgaa
     1081 atggcgcagt ggagaactcg ggccgaagtc gaagcacacc gcagatagcc ccgcgatcag
     1141 cagcagcgca gtggccgcgg cctcgcacgc ccactcgaca ggatggaccc cgccgcgtgc
     1201 tgcttgcccc gcggtttgct ccaccttcgg ggccgtgggc ttgcttgtca aagatcgcct
     1261 gtcagctgag gctcgccacg tcaactgcgc tgtgcccact tgagcaactc gtcggcgtcc
     1321 atcgtgttga cgactttcga ttcgtcgacg ccgcactcct gcgcccgcgc gcacccgtag
     1381 atctgccagt cgagctgccc gggcgcgtgc gcatcggtgt cgacggagaa tcggcaacct
     1441 atctcgactg ccatgctcag cagtcgccgt ggcggatcga gtcgttcggg ccggctgttg
     1501 atctcgaccg cgacgtcgtg ctgtttgcac gctgcaaaga ccttctcggc gtcgaactgc
     1561 gactcgagcc gcgacttgcc gccgtcccga ccgatcagga gccggcccgt gcaatgcccg
     1621 agcacgtcga caagcggatt gcggacggcg gcgagcatcc gtcgcgtcat cgcgatgcgg
     1681 tccatcttga gtttcgaatg cacgctcgcg accacgatgt cgagttggtc gagcaactcg
     1741 ggctcctggt ccagcgaccc atcgtcgagg atgtcgcact cgatgccggt caggatccga
     1801 aacggcgcga gcttctcgtt cagctcggcc accacgcgta gctggtcgcg cagccgctcc
     1861 gcggtgagtc cactcgcgac cgtgagccgc ggcgagtgat cagtcaaggc gatgtagtca
     1921 tgcggaccgc tcgccaagtc cctggctgcc aaagccattt cctcgatcgg actgccgcca
     1981 tcactccagt ccgagtgcgt gtgtaggtcg ccgcgcaacg tcgcgcgcaa gctctcgccg
     2041 ccctcgacga gcggctcgtc acccgcaggc agcagcttcg cgaggtacgc gggcacggta
     2101 cctgcaagtg cttcgcgcgc caccgtcgcc gtgacagccc cgacaccggc aaggtcggtt
     2161 agtgtgcctc gcttcgcgcg ctgctccagt tcgccttccg gcaaggcggc gaggaccgcg
     2221 gctgcggtgc ggaaggcctt gacgcgatac gtcggttcgc gcgcccgctc gagcagaaac
     2281 gcgatctgtc gcaacgcgtc aatcggatcc ggtgcgccga tcgggtcggg tacctcttcg
     2341 cccatccagc tatcgtggca cggcgttgtc gtggtcgatg tggcctgcgg gccgtggcga
     2401 cccgaacacc agcaccgacg ccgagaagcc gtgcagatgg ttgcggttgc cgacaggtcc
     2461 gatctcgcct gacccgaaga accccgcgat acccgtggtc gcgagaccct gccgcaacgc
     2521 gaggacgtcg tggtcagccg acgtgaacag gttggagccg cgcccgttgc aggagaacag
     2581 cagcgcgccc tcgacggcac tgaactcatg cttgagttgg accaacatcg cccgcaaatc
     2641 gtcgtccgcc gaagcagcgt cacggacctg gaaccgcacg gtgcggccca ccggcacgat
     2701 gtcgccgatc gcgatcgcgc cgcgcgcctt gtcgatgccg aggatgccgc ggaccaggaa
     2761 gtcgccgcgc tcgtgctcct cgacgtactc gtccattgcg atgccgattt gcaggccgcc
     2821 gctggcgagc gcctgatcga tcgacgaaag cgagccgatc acctgctcga gtttctggat
     2881 cgccgggatg cccgcaagtt cgaacagcag gtttccctcg gcggccgtca ccgtcatcgc
     2941 cggcccgacc ggccggcagc cttggctgac catcgcctgc gcaccgactg gaccaccgag
     3001 cacaacaccc accgcaccgc ggtcgaccac ccggccgtcg atcagcagtc gggtggagcc
     3061 ggggccgcgg ttgccggcgg ccaatccacc gaccagtggc agggcaagcg cggtgttcgc
     3121 ctgctcgacg aaaccgtccg ccgggaagct gtacggatcg gccacgacga cggcgacctc
     3181 gtcggcgtcc tcgctttctg gcagcccgag gatcgccatg ccaccttcgg tgcggatcac
     3241 ttcaagatga aaagccctga cccggacgcc ggggagcacc gccgcccaga cgctgaccgc
     3301 gctggtcgct tcgatgccct gtgccgcgcc gatgactccg gacgcgctgc agccgatcac
     3361 ggtccgggcg cgaaggacgc ttgccgcgcg ctggagggct tcgccgacct gatcgggctc
     3421 ggatccgcag acaaagacgc aggccaggtc ggggctgagg ccgcccagcg gcccgcgcgc
     3481 ggcgatcgcg gcagcctcgg cggcgcgcac gagatcggcg tcgatcgcga gaccgtcccc
     3541 aaaacgcgcc actgcccgat cctgctgtct gttggtccgg atatctcatt ccccggggtc
     3601 ctatccaccc ggccgtccta tcctcccggc ttacggcacg cctgtcatgc agttgccccg
     3661 cgcatccggg tgacgcgcgc tccgcaaccg cccggccccg gaccgtaacc gacgccaagg
     3721 ctcaggtgat cacgtcggcg atggggaagt cgccttggat ctgcgacgag tcgaagtcga
     3781 atggacagtg cgacatgtca ccgccggata ccagatagag ggtgtaagac ccgcctttag
     3841 gcagcgcgtt gagcacggca gctggcagca cgagcgggcc ctgcctgaac cggtcgagag
     3901 tcaccctcgc cacgtggggt tcggtaccga ccgcgagttt gcggtacgaa tccacaaggg
     3961 ccgaaacggc agccccatag gcatccgacg gcggcgagag agtggggtcg ataggcggtc
     4021 gcttcacgat gacctcgagc cggtaatgga gggtggtcgt cccggcaccc ggcggctcga
     4081 agtcgactcc cacgtcgccg aaaggcctgg tgatccttgc caggtcgtcc gtgggcatga
     4141 cgacgtgcgg accgtagatc ggcaggtaag tcggactcgg gcacgttggg gttgcgacgt
     4201 agagaggacc ggtcggcgcg gtcgcggtgg ctgaagcctc ggcagatgcc gaggcaatgg
     4261 ttgccaccgc gacccttgtg ctgggaggcg cgatgttgac gggcgcggtg ctggcgggcg
     4321 tcgtgctcgc gggcgccgtg ctggcagccg gtggcctggc ggcagcggtt gcgttggccg
     4381 ctggggttgt cgcggccgac gttgtagccg cgacgccagc gcgtgacgag catccggaca
     4441 gcaatccgac gg
//
LOCUS       DATCBJ010000329         2273 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_258509, whole genome shotgun sequence.
ACCESSION   DATCBJ010000329 DATCBJ010000000
VERSION     DATCBJ010000329.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2273)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2273)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2273
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_258509"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(85..471)
                     /locus_tag="VII50_06545"
     CDS             complement(85..471)
                     /locus_tag="VII50_06545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PAAR domain-containing protein"
                     /protein_id="HEY5050541.1"
                     /translation="MGTPAIVMGDQIMGTCPIHIIPNPATGVPQPSPPLPFSAPVTVG
                     VCPTVLIAGKAAAVVGCTGFNTPPHVGLFPADPYMVPVTQVGTIVSGSTTVLIGGQPA
                     ATASSSCTCCATPGTITPTVTSVLIG"
     gene            complement(473..>2273)
                     /locus_tag="VII50_06550"
     CDS             complement(473..>2273)
                     /locus_tag="VII50_06550"
                     /inference="COORDINATES: protein motif:HMM:NF033848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="VgrG-related protein"
                     /protein_id="HEY5050542.1"
                     /translation="AHRPRNRPAHPAGSTLRWLAMMTADALTSWPGVEIDGSPLSDDL
                     RIGLTGVVVDNDIHLPDMFTMIFDDPQHDLLTEAGVRVGSAVTIKASSVGSTDSDVLI
                     GGEVTALEASYENGWDRTIVRGYDKSHRLHRGRRTETYQNVKVSDVAQTVATRAGLDV
                     GTIDDTGSVLPFISQVNITDWEFLQARARELGFEVGVVAGQFNFRRPVESSTGPEPGD
                     SDSHGAEQLVYGRDLVSFRPRISSSAQVTQISVRAWDPDSKTVLVGSADPVASHADLT
                     DDPASLAQNFGSPEYVVHNRPLPSQSDVDDTAKAVAEHVGSSFAEAEGVAVGNTKLLA
                     GTAVSIAQVADTFQGKYTLSRTRHVFDDDGYRTEFEISGLHDRSTLGLMSLGATNGRA
                     AGGAPPVPGVFIGQVTNNDDPQQLGRLKLKFPWLSDSYESDWARVAVVGAGPNSGVVF
                     LPEVDDEVLCACEFGDLRRVFVLSPLHNGKDKPSLGDGLVDAGKVKRRGMVSRKGHRV
                     VFLDDDSKSGIALISSDGNLKVSLNETNSEITIHCKGKVTLETDSDDISIKSGADVTI
                     QAQGNVNVSGSGGVKVDGGGGVVEMTGSLIKLN"
BASE COUNT          359 a          748 c          768 g          398 t
ORIGIN      
        1 accagcgcga tcccgccggt tgcgtcggtg cgcagcggga agccccagcc ggaccctatg
       61 aaatcgtcac ttttggccat aacttcaccc gatgagcacg ctggtaactg tgggagtgat
      121 ggtcccggga gtggcgcagc aggtgcacga tgaggaggcg gtcgccgcgg gctggcctcc
      181 gatgagcacc gtggtgctcc cgctcacgat cgttccgacc tgcgtcaccg gcaccatgta
      241 gggatcagcg gggaagagtc caacgtgcgg cggcgtgttg aagcccgtgc agcccaccac
      301 cgccgccgcc ttgcccgcga tgagcaccgt cgggcacacg cccacggtga ccggcgcgct
      361 gaacggcagt ggcggtgagg gctgcggaac gccggtcgcg gggttgggga tgatgtggat
      421 cggacacgtc cccatgatct gatcgcccat gacgatcgcc ggtgttccca tctcagttca
      481 gcttgatgag tgagccggtc atctcaacga caccgccgcc gccgtccacc ttgacgccgc
      541 ccgagccgga gacgttgacg ttgccctggg cctggatggt cacgtcggca ccgctcttga
      601 tcgagatgtc gtcgctgtcg gtttccagcg tgaccttacc cttgcagtga atcgtgatct
      661 cgctgttcgt ctcgttcaat gacaccttga ggttgccgtc gctgctgatg agcgcgatgc
      721 cggacttcga gtcgtcgtcg agaaacacga ctcggtggcc tttgcgcgac accatcccgc
      781 gacgcttgac cttgccggcg tcgacgagtc cgtcgccgag gctgggcttg tccttgccgt
      841 tgtgcagcgg gctcagcaca aacacgcggc ggagatcgcc gaactcacag gcgcacagca
      901 cctcgtcgtc cacctcgggg aggaacacca cgccgctgtt cggcccggcc ccaaccacgg
      961 cgacacgcgc ccagtcgctt tcgtagctgt cggagagcca cgggaacttc agcttcaacc
     1021 ggcccagctg ctgcgggtcg tcgttgttgg tgacctggcc gatgaagacc cccggcaccg
     1081 gcggcgcgcc tcccgccgcg cggccgttcg tggcgccgag cgacatgagg ccgagcgtcg
     1141 accggtcgtg caagccgctg atctcaaact ccgtccggta cccgtcgtcg tcgaagacgt
     1201 gacgagtccg gctcaacgtg tatttgccct ggaaggtgtc cgcgacctgg gcgatgctca
     1261 ccgccgttcc ggcgagcagc ttggtgttgc ccacggcgac gccctcggcc tcggcgaacg
     1321 agctgccgac atgctcggcc acggccttcg cggtgtcgtc gacatccgac tgcgacggca
     1381 gcgggcggtt gtgcacgacg tactccgggg acccgaagtt ctgggcgaga ctggcaggat
     1441 catcggtgag gtcggcgtgc gacgcgaccg ggtcggcgga tccgaccagc acggtcttgc
     1501 tgtcgggatc ccaggcgcga acgctgatct gggtcacctg agccgatgag ctgatccgcg
     1561 ggcgaaagct gacgaggtcg cgtccataga caagctgctc ggcgccgtgc gagtccgaat
     1621 cgccgggctc ggggccggtg ctcgactcga cggggcggcg gaagttgaac tgccccgcga
     1681 ccacgccgac ctcgaagccg agctcgcgcg cccgcgcctg gagaaactcc caatcggtga
     1741 tgttcacctg cgagatgaaa ggaagcaccg agcccgtgtc atcgatcgtg cccacgtcga
     1801 gcccggctcg cgtcgcgact gtttgcgcca catctgacac tttcacgttt tgatatgttt
     1861 cggtgcggcg gccgcgatgc agccggtgcg acttgtcgta gccgcgcacg atggtgcggt
     1921 cccacccgtt ctcgtagctc gcctcgaggg ccgtgacctc gccgccgatg agcacgtcgc
     1981 tgtccgttga cccgacactc gaggccttga tggtcaccgc cgagccgact cgcacccccg
     2041 cctccgtgag gagatcgtgc tgcgggtcgt cgaagatcat cgtgaacatg tcggggagat
     2101 ggatgtcgtt gtcgacgaca acaccggtga gcccgatgcg gaggtcgtcg gacaggggac
     2161 tgccgtcgat ctcgaccccc ggccagctgg tgagggcgtc ggcggtcatc atcgcgagcc
     2221 accggagtgt ggatccggcg ggatgagcag ggcggttccg ggggcgatgc gca
//
LOCUS       DATCBJ010000330         3417 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_252847, whole genome shotgun sequence.
ACCESSION   DATCBJ010000330 DATCBJ010000000
VERSION     DATCBJ010000330.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 3417)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3417)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3417
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_252847"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..326
                     /locus_tag="VII50_06555"
     CDS             <1..326
                     /locus_tag="VII50_06555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEY5050543.1"
                     /translation="KLDQVLANLVDNAVRHGAGTVTISIAAADDGIVVAVADEGDGVA
                     ADIRNLVFRRFWRAGRRGGTGLGLYIVKGLVEAHGGQVAVLDAPEGGAAFRIALPTGT
                     PAFAL"
     gene            365..1474
                     /gene="pheS"
                     /locus_tag="VII50_06560"
     CDS             365..1474
                     /gene="pheS"
                     /locus_tag="VII50_06560"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HEY5050544.1"
                     /translation="MSAPNSNYDPVEVSPLHADEVERFRDEALAAIGDAADLDQLKEL
                     RLAHAGDRSPLALANREIGALPPQAKADAGRRVGQARAAVAAALAERESVLLAQRDAR
                     ILVEEAVDVTMPWDRAPRGARHPISTLTDRIVDVFVSMGWGVAEGPEVEAEWFNFDAL
                     NTPADHPARALADTFFIESADSGVVLRTHTSPVQARTLLTREPPIYIVAPGRTFRTDE
                     LDATHTPVFNQCEGLAVDEGLTMANLRGTLDAFAEAMFGVGVVTRLRPHFFPFTEPSA
                     EVDLRCWVCHGSSVGHPENPCRTCRSEGWIEWGGCGMVNPRVLIACGVDPQRYTGFAF
                     GMGIERTLLSRHGGGDMHDVVEGDVRFALAFGPES"
     gene            1437..>3417
                     /gene="pheT"
                     /locus_tag="VII50_06565"
     CDS             1437..>3417
                     /gene="pheT"
                     /locus_tag="VII50_06565"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HEY5050545.1"
                     /translation="MCASPSRSARSPEVRVGLSWLRAVVAVPAAEGGHEVADRFVRAG
                     FEVDSVEVEGSVSGPLVIGEVVSFDDEPQTNGKTIRWCQVRVADGAQPRGIVCGAHNF
                     AAGDRVVVALPGAVLPGGFEISARKTYGHLSDGMICSVRELGIGDDHTGILVLPPDAP
                     LGADAVAVLGLGDEVLDVSPTPDRGYAMSVRGLARELAASYRIPFDDPALAVTVAAGS
                     ADGGWPVRIHDASGCDRFVAQTVRGIDPSRSAPDWIRRRLQTAGVRSVSLAVDVTNYV
                     MLELGQPLHAYDLAKLRGPIVVRRALPGERVRTLDDVDRTTHPDDLLIADDSGAIGIA
                     GVMGGATTEIDATTSQVLIEGAHFTASVISRSARRHGLLSEASRRFERGVDPAVASVA
                     VQRAADLLAEFGGGTTESTSTDVGERPPPSTIRIGATLAAEVVGVDYDLDEVLGTLLA
                     VGCEVEQAGAELSVTVPSWRPDLRDPYDLVEEVARLAGYDRIPSVVPAAPRSGGLTEA
                     QRARRRIAMALADAGYVEAPSYPFIGPADLDALGLGADDPRRRTLRLANPISDEQPDL
                     RTTLLPGLLSALRRNIARGNTDVALFEAGLVYRIDDRENGLVRPPRLSVDRRPTDDEL
                     AALNAALPRQPRRLAVALAGERERSGWWGSGRPVEW"
BASE COUNT          498 a         1157 c         1184 g          578 t
ORIGIN      
        1 acaagctcga tcaggtactt gccaacctcg tcgacaacgc cgtacgccac ggcgcaggga
       61 cggtcaccat ttcgatcgcc gccgccgacg acggaatcgt cgtcgcggtc gccgacgagg
      121 gcgacggcgt cgccgccgac atccgcaacc tggtgttccg ccgcttctgg cgagccggcc
      181 gccgcggcgg taccggactc ggcctgtaca tcgtcaaggg gctcgtggaa gcgcatggcg
      241 ggcaggtcgc ggtcctcgac gcgccagaag gcggggcagc ctttcggatc gcgttgccga
      301 ccgggacgcc cgcgttcgcc ctctagactc accggtgatc ccgcacacct tggagagtcg
      361 ccccgtgtcc gccccgaaca gcaactacga ccccgtcgag gtgagtccac tgcacgccga
      421 cgaggtcgag cggttccgcg acgaggcctt agccgcaatt ggtgacgccg cggatctcga
      481 ccagctgaaa gagctgcggc tggcgcacgc cggcgaccgg tctccgctcg ctctggcgaa
      541 ccgcgagatc ggcgcactgc cgccacaagc caaagccgat gccggacggc gggtgggcca
      601 ggcccgagca gccgtagcgg ccgcactcgc ggagcgggag tcggtgctgc tcgctcagcg
      661 cgatgcgcgg atcctggtcg aggaagcggt cgacgtcacg atgccttggg atcgcgcgcc
      721 gcgcggcgcc cggcacccga tctcgacgct gaccgaccgc atcgtcgacg tgttcgtctc
      781 gatggggtgg ggcgtcgccg agggccccga ggtcgaggcg gagtggttca acttcgacgc
      841 actcaacacg cccgccgatc acccggcgcg agccctggca gacacgttct tcatcgagtc
      901 cgccgatagc ggcgtggtgt tgcgcaccca cacgtcaccc gtgcaggcgc gcaccttgct
      961 gacgcgcgag ccgcccatct acatcgtcgc ccccggtcgt acgtttcgca ccgacgagct
     1021 cgatgcaacc cacacgccgg tctttaacca atgcgaggga cttgccgtcg acgaggggct
     1081 gacgatggcg aaccttcggg gcaccctcga cgcattcgcc gaggcgatgt tcggcgtcgg
     1141 tgtggtgacc cgattgcggc cgcacttttt ccccttcacc gaaccgtctg ccgaggtcga
     1201 tttgcgttgc tgggtgtgcc acggttcctc ggtcggtcat ccggagaacc cgtgccgcac
     1261 ctgccgatcg gaaggctgga tcgagtgggg cggctgcggc atggtaaacc cgcgggtgct
     1321 gatcgcctgt ggtgttgacc cgcagcgtta caccgggttc gcgttcggta tgggtatcga
     1381 gcgcacgttg ctgtcacgcc acggcggcgg cgacatgcat gacgtcgtcg agggcgatgt
     1441 gcgcttcgcc ctcgcgttcg gcccggagtc ctgaggtgcg tgtcggcctg tcgtggttgc
     1501 gtgccgttgt cgcggtcccg gctgctgagg gaggccacga ggtggccgac cggttcgtgc
     1561 gcgccggttt cgaggtcgac tcggtcgagg tcgagggctc ggtgtccggc ccgctcgtca
     1621 tcggtgaagt ggtgtcgttc gacgacgaac cgcagacgaa cggcaagacg atcaggtggt
     1681 gccaggtccg agtcgccgac ggtgcgcagc cgcgcggcat cgtctgcgga gctcacaatt
     1741 ttgcggccgg cgatcgcgtc gtcgtcgcgc tgccaggggc ggtgttgccg ggcgggttcg
     1801 aaatctccgc gcgtaagacg tacggacacc tcagcgacgg catgatctgc tcggtgcgcg
     1861 agctcggcat cggcgacgac cacacaggca tcttggtgtt gccgccagac gcgccgcttg
     1921 gagccgacgc ggttgccgtg ctcgggctcg gcgatgaggt cctcgacgtt tcgccgaccc
     1981 ccgaccgcgg ctacgcgatg tcggtgcgcg gcctcgcccg cgaactcgcg gcttcctacc
     2041 gcatcccgtt cgatgacccg gcactcgctg taacggtcgc cgccggcagt gccgatggcg
     2101 ggtggccggt gcgcatccac gacgccagcg gttgcgatcg cttcgtcgcg cagaccgtgc
     2161 gcggcattga cccgagtcgc agcgcacctg actggatccg acgtcggctg cagaccgccg
     2221 gagtccgttc ggtcagcttg gcggtcgacg tcacgaacta cgtcatgctc gaactcgggc
     2281 agccgttgca tgcctacgac ctggcgaagc tgcgcgggcc gatcgtggtt cgtcgcgcgc
     2341 tgccaggcga gcgggtccgc actctagacg acgtcgatcg cacgacgcat ccagacgatc
     2401 tgctcatcgc cgacgacagc ggagccattg gcatcgccgg cgtcatgggt ggtgcgacga
     2461 ccgagatcga tgccacgacg tcacaggtcc tgatcgaagg cgcccacttc accgcgtccg
     2521 tcatttcgcg aagcgctcga cggcacgggt tgctgagcga agcctcccgc cggttcgaac
     2581 gtggggtcga tcctgccgtc gcgtcggtcg cggtccagcg cgcggcggat ctccttgccg
     2641 agttcggcgg cggcaccacc gaatccacat cgacggacgt cggcgagcgg ccgccaccgt
     2701 cgacgatccg aatcggcgcc acgctggccg ccgaagtcgt cggagtcgac tacgaccttg
     2761 acgaagtgct cggcacattg ctggcggtgg gctgcgaggt cgagcaggcc ggcgctgagt
     2821 tgtcggtcac ggtcccgagt tggcgccctg acctgcgtga cccctacgac ttggttgagg
     2881 aggtcgcgcg gctcgctggc tacgaccgga tcccgtcggt cgtgccggca gcgccacgaa
     2941 gtggcggact caccgaagcg caacgcgccc gccgccgcat tgcgatggcg ctggccgacg
     3001 cgggctacgt cgaagcgccg agttatccgt tcatcggtcc ggctgacctc gacgctcttg
     3061 gactgggtgc cgacgatccg cggcggcgca cgctgcggtt ggcgaacccg atctccgacg
     3121 agcagcccga cctgcgcacg acgttgttgc ctgggttgct gtctgcgctg cgtcgcaaca
     3181 tcgcgcgcgg caataccgac gtcgcgctct ttgaagccgg actcgtgtac cgcattgacg
     3241 atcgcgagaa tggcttggtt cgtccgccac gattgtccgt cgatcgccga ccgaccgacg
     3301 atgagcttgc ggcgctcaac gcagcattgc ctcgacaacc gcgccgactt gcggtcgcgc
     3361 tcgctggcga acgcgagcga tcgggttggt ggggctctgg tcggcccgtc gagtggg
//
LOCUS       DATCBJ010000331         3087 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_480195, whole genome shotgun sequence.
ACCESSION   DATCBJ010000331 DATCBJ010000000
VERSION     DATCBJ010000331.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 3087)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3087)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3087
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_480195"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1252)
                     /locus_tag="VII50_06570"
     CDS             complement(<1..1252)
                     /locus_tag="VII50_06570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEY5050546.1"
                     /translation="MAAGARVLRNFVDGAYVDTESGVTSGLVDPSTGEVFATAPVSEA
                     PDVDRAMHAAERAFEVWRDATPSVRQRALLRIADAIEARADEIVAGESQNTGKPLALT
                     ASEELPPMVDQIRFFAGAARVLEGMAAGEYLAGHTSFIRREPVGVCAQVTPWNYPMMM
                     AVWKWAPAIAAGNAVVLKPSDTTPVTTLLMAEIAAEFLPPGVLNVICGERDTGRLLVE
                     HRTPSMVSITGSVRAGMEVAGAAARDLKRVHLELGGKAPVVVFDDADLEKTAEGIAMA
                     VYFNAGQDCTAATRVLAAPAIYNDFVAALTEQARGTVVGPPSNADAAYGPVNNANQLA
                     RVSGFVDRLPDHAQLLAGGHRVGDRGYFYEPTVVAGLQQGDEIVRDEVFGPVITVQKF
                     SDEDEAVRWANSVEYGLSSSVWTRD"
     gene            1419..1898
                     /locus_tag="VII50_06575"
     CDS             1419..1898
                     /locus_tag="VII50_06575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019510115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HEY5050547.1"
                     /translation="MTPRDRPAGPAGVLDEVSKAIIEQLQQDGRRSYATIAQAVGLSE
                     AAVRQRVQRLLKSGVIQIVAVTDPLQLGFPRQAMIGLRVEGDIEAVADELEKFPEVDY
                     VVVTAGSFDLLVETVCEDDDHLLELLNSRIRAIPAVRSTETFVYLKLRKQTYTWGTR"
     gene            1895..>3087
                     /locus_tag="VII50_06580"
     CDS             1895..>3087
                     /locus_tag="VII50_06580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009082328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HEY5050548.1"
                     /translation="MTTPQDVHNADIANASALQEAARRHLWMHFTRMSSYTNHDVPII
                     VRGEGAYVYDSNGRRYLDGLAGLFVSMVGHGRDEIAEAAARQSKELAYFPLWSYAHPR
                     AIELAERLAQLAPGDLNRVFFTTGGSEAVESAWKLARQYFRAIGQPSRYKVISRDIAY
                     HGATMGALSITGLPAIKTVFEPLVPGSVRVPNTNFYRAPEHGDDLVAFGRWAADEIER
                     AILREGPESVAAVYLEPVQNSGGCFPPPPGYFQRVREICDRHGVLLVSDETICAFGRL
                     GYYFAADRYGYQPDIITVAKGMTSGYAPIGAMIASDRIIEPFLKDTATFLHGITFGGH
                     PVSCAVALANLDVFEKEDLLGHVLSTEDAFRATLNKLNDLPIVGEVRGDGYFYGIELV
                     KDKAT"
BASE COUNT          557 a         1055 c          945 g          530 t
ORIGIN      
        1 gatcgcgggt ccagaccgat gacgagaggc cgtattcgac gctgttggcc caccgcactg
       61 cttcgtcttc gtcgctgaac ttctggaccg tgatgaccgg cccgaagacc tcgtcgcgca
      121 cgatctcgtc tccctgctgc aaacccgcta ccacggtggg ctcgtagaag tagccacggt
      181 caccaacgcg gtgcccaccc gccagcagct gcgcatgatc gggcaaccgg tcgacgaatc
      241 cgctgacccg cgcgagttgg ttcgcgttgt tgaccggacc gtacgctgca tcggcattgg
      301 acggcgggcc gacgactgtg ccgcgggctt gctcggtcag tgcagcgacg aagtcgttgt
      361 agattgccgg cgccgccagc accctggtcg cggccgtgca gtcctggccg gcattgaagt
      421 agacagccat cgcaatgccc tcagccgtct tttccaggtc cgcatcatcg aagacgacca
      481 ccggagcctt gccaccgagc tcgaggtgca cccgcttgag atcgcgcgcg gccgcaccgg
      541 cgacttccat gcccgcgcgc acgctaccgg tgatggagac catgctcggc gtgcggtgtt
      601 cgaccagtag gcgcccggtg tcgcgttcgc cgcagatcac gttgagcacg ccgggcggca
      661 ggaactccgc ggcgatctcg gccatcagga gcgtcgtcac cggcgttgtg tcggagggct
      721 taagcaccac ggcgttgcct gcggcgatcg ccggcgccca cttccagacc gccatcatca
      781 tcgggtagtt ccacggcgtc acctgcgcgc acacaccaac cggttcgcgt ctgatgaacg
      841 aggtgtgccc ggccaggtac tcccccgccg ccatcccctc gagcactcgc gccgcgccgg
      901 cgaaaaagcg gatctggtcg accatcgggg gcagttcttc cgatgccgta agcgcaagcg
      961 gctttccggt gttctgcgac tcgccagcga cgatctcgtc ggcccgcgct tcgatcgcgt
     1021 ccgcaatgcg caagagcgca cgttgccgga ccgacggcgt tgcgtcgcgc cagacctcga
     1081 aagcccgctc cgccgcgtgc atcgcacgat cgacgtcggg ggcttcggac accggtgcgg
     1141 tggcgaatac ctccccagta ctcgggtcga cgaggccgct ggtcacgccc gattcggtat
     1201 cgacgtaggc gccatcgacg aagttgcgca gcacgcgggc tccagcggcc atctctatct
     1261 ccttacggcg agggtgccga cagcgtagct ggacgagagc agttgcggga gacgagaaca
     1321 tcagtgatat cgacgatcga ccattgattc cgtggatgcg ctggcccgtg actgcggaat
     1381 cgcttgcacc gcgtcaacgt gcgggccatg atggcgagat gaccccccgc gaccgaccgg
     1441 cgggacccgc cggcgtcctc gacgaggtct cgaaagccat tatcgaacaa ctccagcagg
     1501 acggacgccg ctcatacgca acgatcgcgc aagccgtcgg gctctcggag gccgcggtac
     1561 gacaacgcgt tcagcggttg ctcaagagcg gtgtcatcca gatcgtggcc gtcactgacc
     1621 cgctccaact ggggttcccg cgccaggcga tgatcggttt gcgtgtcgaa ggcgatatcg
     1681 aagcggtcgc cgatgaactg gaaaagtttc ccgaggtcga ctacgtcgtc gtcaccgcgg
     1741 gctcgttcga cctgctggtc gaaaccgtct gcgaggacga cgaccacctg ctcgagcttc
     1801 tgaacagtcg gatccgggcc atcccggccg tccgcagcac cgagaccttc gtctatctca
     1861 agctccgcaa gcagacatac acctggggga ccagatgacc acgccacaag acgtccacaa
     1921 cgccgacatc gccaatgcga gcgcattgca ggaggcggcc cgccgccacc tctggatgca
     1981 cttcacccgg atgtcctcct atacaaacca cgacgtgccg atcatcgtgc gcggcgaagg
     2041 cgcgtacgtc tacgacagca acgggcggcg ctacctcgac ggactcgccg gcctgttcgt
     2101 cagcatggtc ggtcacgggc gcgacgagat cgccgaggcc gctgcccggc aatcgaagga
     2161 gctggcatac tttccgctgt ggagttacgc ccatccacgc gcgatcgagt tagccgagcg
     2221 gttggctcag ctggctcccg gcgaccttaa ccgggtgttc ttcaccaccg gcggctccga
     2281 agctgtcgaa tcggcgtgga agctggcacg ccagtacttc cgtgccatcg gccagccgag
     2341 ccgctacaag gtcatcagcc gagacatcgc ttatcacggt gcgaccatgg gcgcgctctc
     2401 gattaccggc ctgccggcca tcaagactgt gttcgagccg ctggtaccgg gttcagtgcg
     2461 ggtaccgaac accaacttct atcgcgcccc ggagcacggc gatgacctcg tcgcgttcgg
     2521 ccgctgggct gcggacgaga tcgaacgcgc catcctgcgc gaaggccccg aatcagtcgc
     2581 ggcggtatat ctcgaacccg tgcagaactc gggcggctgt ttccctccac cgcccgggta
     2641 tttccaacgc gtacgcgaga tttgcgaccg ccacggcgta cttcttgtct ctgacgagac
     2701 catctgcgcg ttcggtcgac tcggctatta cttcgctgcg gatcgctacg gataccagcc
     2761 cgacatcatc accgtcgcca aaggcatgac gtcgggatat gcaccgatcg gcgcgatgat
     2821 cgcatcggac cggatcatcg aacccttcct caaggacacc gcaaccttcc tgcacggcat
     2881 caccttcggc ggccacccgg tcagctgcgc ggtggccctc gcaaacctcg acgtcttcga
     2941 gaaagaagac ctgctgggtc acgtgctttc gacggaagac gccttccgcg ccactctgaa
     3001 caagctcaac gacctgccca tcgtcggtga ggtccgcggc gacggatact tctacggcat
     3061 cgaactcgtg aaggacaaag cgacgag
//
LOCUS       DATCBJ010000332         2344 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_490401, whole genome shotgun sequence.
ACCESSION   DATCBJ010000332 DATCBJ010000000
VERSION     DATCBJ010000332.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2344)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2344)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2344
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_490401"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..220)
                     /locus_tag="VII50_06585"
     CDS             complement(<1..220)
                     /locus_tag="VII50_06585"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY5050549.1"
                     /translation="MPDPDVIATTGRVILRPWRVAEADRFFDIYRRPEVTDWLGAPPM
                     VDRQEAVAMIERGIERRAADPRFGGWAVI"
     gene            complement(348..818)
                     /locus_tag="VII50_06590"
     CDS             complement(348..818)
                     /locus_tag="VII50_06590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5050550.1"
                     /translation="MTSKADFNAEEWSTVVDGPLYAGMRVISADRGGTVRESLAMGRA
                     YQHAREHHGESELLDELVKSPPSIDADRVREAGGDIAGVASQQLRDAIAILGAKSTAA
                     DVDAYKRFVMTVAQAVAGAHKEGGFLGIGGKQISDAENRALDEISAALGAPPAA"
     gene            955..1452
                     /locus_tag="VII50_06595"
     CDS             955..1452
                     /locus_tag="VII50_06595"
                     /inference="COORDINATES: protein motif:HMM:NF013933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family translocator"
                     /protein_id="HEY5050551.1"
                     /translation="MRNSADEPHVSRHQFGHRGFARDRCSAHRVGRSWPRRARERGHG
                     AGLHPGDRSPLVLDGLAARESHRRVIGSAILANLLNPKLTIFFFAFLPQFVPEHSSAQ
                     LMRMLGLNAVFMAMTFIVFVGYGVFAAGVRRHLIERPRIVRRVRQAFAVSFVALGAKL
                     AVTAR"
     gene            1690..>2344
                     /locus_tag="VII50_06600"
     CDS             1690..>2344
                     /locus_tag="VII50_06600"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HEY5050552.1"
                     /translation="MGAGVALGPNAMKVLRALGLEDAVRAVAGRSEWAVTRNGRTGRT
                     ISKTSRQHQAVVFGSASATVHRADLLDVLAQSLPSDIVTLGARCTGVEPGGGVPVARF
                     QDGSEVEADVIIGADGIHSAVRTSLFGSDAPRFTGKVCYRSVVPVDAVPGEPPANDNA
                     QWLGPHGTIVLYPMRRNELINVVCHHDDETYRHESWVSECERAEVLERHGGWHESLLR
                     "
BASE COUNT          330 a          808 c          810 g          396 t
ORIGIN      
        1 cgatcaccgc ccacccgccg aaccgcggat cggcggcgcg ccgctcgatg ccgcgctcga
       61 tcatcgccac cgcctcttga cggtcgacca ttggcggtgc accaagccaa tcggtgacct
      121 ctgggcggcg atagatgtca aagaaccgat cggcctccgc gactcgccac ggacgcagga
      181 tgacccgccc ggtcgtcgcg atcacgtcgg gatcaggcat gagcgcgagt ctgccatggc
      241 atcagctccg cctcacccat gcgcgctcgc cgcctgtggt tccttggtga gtcggccgtg
      301 gttgacgcgg gtgcagcgtg tcggtcttgc cgacccgcgt gggccggtca ggccgccggg
      361 ggcgcgccga gcgcggcgga gatctcgtcg agcgcccggt tctcggcgtc gctgatctgc
      421 ttgccgccga tgccgaggaa gccaccctcc ttgtgagcgc cggccaccgc ctgcgcgacg
      481 gtcatcacga accgcttgta cgcatcgacg tccgcggccg tcgacttcgc accgaggatc
      541 gcgatcgcgt cgcgcaactg ctgcgacgcg acgccggcga tgtccccgcc cgcctcacgg
      601 acccggtcgg catcgatcga cggcggcgac ttgaccagct cgtcgagcaa ctcactctcc
      661 ccgtggtgct cgcgggcgtg ctggtaggcg cggcccatcg ccaggctctc acgcacggtg
      721 ccgccacggt cggcgctgat cacgcgcatg ccggcgtaga gcggcccgtc gacaacggtg
      781 gaccattcct cggcgttgaa atcggctttg cttgtcattc gagtcctctg cgatcgtcgg
      841 agcgctcaac tgggagcatt gtgcccgagc gggatcttca cgcaagcccg agcggcgacg
      901 cgacccgccg gctcggtcgc gcagtccgag gcctccggtg cgacgcgccg caacatgcga
      961 aactcggctg acgagcctca cgtttctcgt caccagtttg gtcatcgtgg ctttgcccgg
     1021 gaccggtgct ctgctcaccg tgtcggcagg tcttggccac ggcgcgcgcg cgagcgtggt
     1081 cacggcgctg ggctgcaccc cggggatcgg tcaccgctcg tcctcgatgg gctcgcggca
     1141 cgcgagtcac accggcgggt catcggctcc gcgatcctgg ccaacctact caatccgaag
     1201 ttgacgatct tcttctttgc gttcctgccc caattcgtgc ccgaacacag ctccgcccag
     1261 ctaatgagga tgctcgggtt gaacgccgtg ttcatggcga tgaccttcat cgtgttcgtc
     1321 ggctacggcg tcttcgccgc gggcgtgcgc cgccacctca tcgagcgtcc ccgaatcgtc
     1381 cggcgcgtcc gccaggcgtt cgccgtgtcg ttcgtcgccc ttggggccaa gctcgccgtg
     1441 accgcccgct gatcgcgagg tcacggccgt gatcgccggc ctgcgtggcg gtgccggtcg
     1501 tgtgaccacg gtacgatgcc cactgctgag cgcttgtggt gagaagtgat gaaatgacac
     1561 cggtggcgga ctgaatgacg cgggttgcga tcatcggcgg cggcatcggc ggtctgacct
     1621 cgatgccgcg cgctgtcgct gcgcggcatc gaggtcgccg tctacgaggc agcgcctgag
     1681 ctgcgtgaga tcggcgcggg ggtcgcactt ggcccaaacg ccatgaaggt gctgcgggcg
     1741 ctcggtctcg aggatgcggt ccgcgccgtc gcggggcgct ccgagtgggc ggtcacgcgc
     1801 aacgggagga ccggccggac gatctcgaag acgagccggc agcaccaggc cgtcgtgttc
     1861 gggagtgcga gcgccacggt gcatcgcgcc gacctgctcg acgtgctggc gcaatcgttg
     1921 ccgagcgaca tcgtcacgct gggagcgcgc tgcaccgggg tcgagccggg gggtggggta
     1981 cccgttgccc ggttccagga cggcagcgag gtcgaggccg acgtcatcat cggcgccgac
     2041 gggatccatt ccgccgtgcg aacgtcgctg ttcggctcgg acgctccgcg cttcaccggc
     2101 aaggtgtgtt atcgcagcgt ggttccagtc gacgccgtgc ccggcgaacc gcccgcgaac
     2161 gacaacgcgc agtggttggg gccccacggc acgatcgtcc tgtaccccat gcggcgaaac
     2221 gagctgatca acgtggtctg ccaccacgat gacgagacgt accggcacga gtcgtgggtc
     2281 tcagaatgcg agcgcgcgga ggtgctcgag cggcacggcg gctggcacga gtcgctgctg
     2341 cggg
//
LOCUS       DATCBJ010000333         2670 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_73751, whole genome shotgun sequence.
ACCESSION   DATCBJ010000333 DATCBJ010000000
VERSION     DATCBJ010000333.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2670)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2670)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2670
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_73751"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(30..770)
                     /locus_tag="VII50_06605"
     CDS             complement(30..770)
                     /locus_tag="VII50_06605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016460401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEY5050553.1"
                     /translation="MPTNAVPAPHRVLVVDDEPSISDLVATALRFEGFDVSTASTGNG
                     ALEAVEMSRPDLVVLDVMLPDFDGFEVARRLAAKGKSVPVLFLTARDATDDKVLGLNV
                     GGDDYVTKPFSLAELVARVHAVLRRSHGNGHGTRISFSDLEMDDDTREVFRGGRLIEL
                     TATEYRLLHYLLVNARKVLSRAQLLDHVWDYDFGGDSGVLETYISYLRKKVDVDGPPL
                     IHTVRGVGYTIRLSNRPAETVARATEAI"
     gene            complement(961..2043)
                     /locus_tag="VII50_06610"
     CDS             complement(961..2043)
                     /locus_tag="VII50_06610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009085225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEY5050554.1"
                     /translation="MTNESQTTEPLRVAIVGYGLAGSVFHAPLIAATPGMRVSAVVTG
                     NPERSDKARQTYEGVEVFADVATLLAKPADIDLVVVASPNCMHAPIALAAIDGGIAVV
                     VDKPFAVNVGEGESLIAAAAARGVPLSVFQNRRWDGDFLTLRRLLDEGALGEVTRYES
                     RFERWRPVAGAGWRESGSPSDAGGLLFDLGPHLIDQALQLFGPVTHVYAEVDRRRAGV
                     QADDDTFVALTHASGVRSQLWCSAIASDLGPRMRVLGTRSAYVKYGLDVQEDALRRGG
                     RPDRPDTPAGWGTESEASYGTLGVVGEATPIATEPGAYQRYYEGIAAALRGEGDLPVD
                     PRDSVEGLRIIEAARRSHEQEQVVSL"
     gene            complement(2071..2595)
                     /locus_tag="VII50_06615"
     CDS             complement(2071..2595)
                     /locus_tag="VII50_06615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY5050555.1"
                     /translation="MGIFRLNHAVLYVRNLDESVRFYTDVLGFRAINMTPEGFRGAAF
                     LQAPGSTNDHDLGLFEIGAAGPSGAGRTTVGLYHLAWEVDTLLDLERTAAALSAADAL
                     VGSSDHGSTKSLYGRDPSGLEFEIVWLIPADLLDDDVLEARRSIGHLDIEKEKARYGA
                     TTQGGIGISRVAVS"
BASE COUNT          499 a          886 c          852 g          433 t
ORIGIN      
        1 gttcgagcgc tatctagcgc aggagtgagc tagatagctt cggtggcgcg cgcgaccgtc
       61 tcagcaggcc ggttcgacag cctgatcgtg tagccgacac cgcgcaccgt gtgaatcagc
      121 ggcggcccgt cgacgtccac cttcttgcgc aggtagctga tgtacgtctc gagcacgccg
      181 gaatcgccgc cgaagtcgta gtcccataca tggtcgagca actgggcacg gctcaacacc
      241 ttgcgtgcgt ttaccaacag gtaatgcagc aaccggtatt cggtagcggt gagttcgatc
      301 aaccggcctc cgcgaaacac ctcgcgagtg tcgtcgtcca tttccaggtc gctgaacgag
      361 atgcgggtgc catgaccgtt gccgtgactg cggcgcaaga ctgcgtgcac acgtgcgacg
      421 agctcagcga gcgagaacgg cttggtcacg tagtcgtcgc caccgacgtt cagaccaagg
      481 accttgtcgt cggtagcatc gcgggcagtc aagaacagca ccgggacact cttgcccttg
      541 gcggccaggc ggcgggccac ctcgaagccg tcgaagtcgg gcagcatgac gtcgagcacc
      601 acaaggtcgg gcctggacat ctcgacggcc tcgagggcac cgtttccggt gctcgccgtg
      661 ctgacatcga agccctcgaa ccgcagggcc gtagcgacca ggtcagaaat gctcggctcg
      721 tcatcgacaa ccagcacccg gtgcggtgcc gggacagcgt tcgtaggcac aggtgaaccc
      781 ttcacaggtc tgcaagtcgg cacacagcgg tagcagttag cacggtggta gcagatagca
      841 cggtggtagc agctacatga gcacccttcc cctacagcct gcaccaagcc tgtgagaaag
      901 ctggaagaat gctgtgagtg gccggtcgac gccgtcctgc cctatggacc gcccgggtca
      961 tcaaagcgag acgacctgct cctgctcgtg cgatcgacgc gccgcctcga tgatcctcaa
     1021 gccctcgacg gagtcgcgcg ggtcgacggg taaatcaccc tcgccgcgca aggcggccgc
     1081 gatgccttcg taatagcgct ggtaggcgcc cggctccgtg gcgatcgggg ttgcctcgcc
     1141 gacgactccc aaggtgccgt aactggcctc gctctcggtt ccccaccccg caggggtatc
     1201 ggggcgatcg ggtcgtcccc cgcgccgcag cgcatcctct tggacgtcga gtccgtactt
     1261 gacgtaggcc gagcgggtgc caagcacccg catgcgtggc ccgaggtcag acgcgatcgc
     1321 gctgcaccac agctggctgc gcacaccgga cgcgtgggtc agcgcgacga aggtgtcgtc
     1381 atcggcctgc accccggcgc gccgccggtc gacctcggcg tagacatgcg tgaccgggcc
     1441 gaatagctgg agcgcctggt cgatgaggtg cgggccgaga tcgaacagca accccccggc
     1501 gtccgacggc gaaccgcttt cgcgccaacc tgcacccgcg accggccgcc aacgctcgaa
     1561 cctcgactcg tatctggtga cctcaccgag cgcgccctcg tcgagcagcc ggcgcagcgt
     1621 caagaagtcg ccgtcccatc gacgattctg aaaaacacta agcgggacgc cgcgggcagc
     1681 cgccgctgcg atcaacgact cgccctcacc cacattgacg gcaaacggct tgtctacgac
     1741 gaccgcgatg ccgccatcga tcgcggcgag cgcgatcggc gcgtgcatgc agttgggcga
     1801 cgccacgacg acgagatcga tgtcggccgg cttcgccagg agagtggcaa cgtcggcgaa
     1861 aacctcgaca ccctcgtagg tctggcgcgc tttgtcagag cgttcggggt tgccggtgac
     1921 gacagccgac acgcgcatgc cgggtgtcgc agcaataagc ggagcgtgga aaaccgaacc
     1981 ggccagtccg tacccgacga tcgcaacacg cagtggttca gtcgtctggg attcattggt
     2041 catgggcaca tagtgccggt cgggcagcgg ttagctgacg gcgacccgcg agatgccgat
     2101 gccgccttgt gtcgtcgcgc cgtagcgcgc cttttccttc tcgatgtcga ggtgcccgat
     2161 gctccggcgc gcctcaagca catcgtcgtc tagaaggtcg gccggaatca gccagacgat
     2221 ctcgaactcg aggccactag ggtcacggcc gtacaagctc ttagtactgc cgtggtcgga
     2281 actaccgacc agcgcatcgg cagccgagag cgccgcggct gtccgctcga ggtcgaggag
     2341 cgtgtcgacc tcccatgcca ggtgatacag accgacggtg gtgcgacccg cgcccgacgg
     2401 gccggccgca ccgatctcga acagcccgag gtcgtgatca ttcgtcgagc ccggagcctg
     2461 cagaaacgca gcgccgcgaa acccctcggg cgtcatattg atcgcccgaa agccgaggac
     2521 gtcggtgtag aaccgcaccg actcgtcgag gttgcgcacg tagaggaccg cgtggttaag
     2581 ccgaaagatt cccatggccg aaccctacct cactttgatt gagcgttcaa ctatcgcgtg
     2641 gcgtgatcgc cgccggcatc tcagcacgcc
//
LOCUS       DATCBJ010000334         3424 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_225389, whole genome shotgun sequence.
ACCESSION   DATCBJ010000334 DATCBJ010000000
VERSION     DATCBJ010000334.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 3424)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3424)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3424
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_225389"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..397
                     /locus_tag="VII50_06620"
     CDS             <1..397
                     /locus_tag="VII50_06620"
                     /inference="COORDINATES: protein motif:HMM:NF036408.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF5313 family protein"
                     /protein_id="HEY5050556.1"
                     /translation="SRRRRESVREMFYGQRVTRIPGDPGLRRWLLFAIGLRLPPENRE
                     WVRHDLTDAGWRSRAAVRQLVLVAPVSAAFAALPGPWSLKVTLMVLVLVGGLLISLMY
                     ADSIRASRLRQHDLPVPEDRDLGRPTDSL"
     gene            complement(407..1117)
                     /locus_tag="VII50_06625"
     CDS             complement(407..1117)
                     /locus_tag="VII50_06625"
                     /inference="COORDINATES: protein motif:HMM:NF015438.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC N-terminal beta barrel domain-containing
                     protein"
                     /protein_id="HEY5050557.1"
                     /translation="MVDRNVARVVSLFRYPVKSMAAEVLDHVDVSWHGLAGDRRWAFI
                     QDGHVRSGFPWLTVRERPVMWRYQPSFADPLRPDKSPTMVRTPAGNELDVTDPILAAE
                     LGPGARVIKHDRGVFDTFPLSLISTRTLADLGATVGMALDPLRFRPNLLVEAIDGAPF
                     PEEAWVGSVLRIGDLRMRLDKRDQRCIMVNVDPQTTKRNAAVLRAIGRDRQACLGVYG
                     STVAPGRVAIGDLVTIEE"
     regulatory      complement(1137..1213)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01057"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="S-adenosyl-L-homocysteine riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="S-adenosyl-L-homocysteine"
                     /db_xref="RFAM:RF01057"
     gene            1329..2099
                     /locus_tag="VII50_06630"
     CDS             1329..2099
                     /locus_tag="VII50_06630"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HEY5050558.1"
                     /translation="MTEFPDFSLDGKVALVTASGRGLGRAIAIALAHAGADVALGLRD
                     RASAGDVVEAITELGRRAVPLQMNMTSLLEIRKATADAHDVFGRLDILVNNAGIAPGA
                     PAASVTEEDFDATLEVNLKGTFFASQAAANIMSDQGSGRIINIGSQAGLVALPGESIY
                     CMTKAAIGHLTKCLAVEWGVLGITVNCVAPTFIETPGTEPALSDPAFRADVVERIAAL
                     HRIGQPVDVTGAVVFLASDAASLITGTTITVDGGWTAR"
     gene            2135..2671
                     /locus_tag="VII50_06635"
     CDS             2135..2671
                     /locus_tag="VII50_06635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5050559.1"
                     /translation="MGVDDDALPVPEGPDAVAEWKARLLAVIVLWRQAAELVFADVAT
                     TTDIRWSWQVALHEDEDDWPARPSDHEPAAYWSLVTQHPPGWEPPRRPLLLPELWLEA
                     AKDATSLGAGYDLNQAVGFVASCLQDWVMDDIQHAWPECPWHQHGADLGNLGGAPTWV
                     CPLGDRLIAEVGKLGGAA"
     gene            complement(2674..2973)
                     /locus_tag="VII50_06640"
     CDS             complement(2674..2973)
                     /locus_tag="VII50_06640"
                     /inference="COORDINATES: protein motif:HMM:NF015735.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HEY5050560.1"
                     /translation="MAEWIYFIHPPRENFAATMTDEEEAVWDEHFQRLKGLLAEGVVV
                     MAGPTLGPKNTGIVVFEAEDEEAAQAIVDADPVFAGGWARAELREFRVSLLRGRP"
     gene            complement(3034..>3424)
                     /locus_tag="VII50_06645"
     CDS             complement(3034..>3424)
                     /locus_tag="VII50_06645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEY5050561.1"
                     /translation="IRERGAGNVTVLAQVLAGAQQDANLAAAAKHALSLWIAEIETTL
                     VRLLKGSPLSEVANPEGLARGIAASFIGIELFEGVDPEGAQNAIASLETLGILVEVVE
                     DLGPVARRALRTRVRRTARPRRNGSVD"
BASE COUNT          599 a         1104 c         1129 g          592 t
ORIGIN      
        1 ctcacggcga cgtcgagaat cagtccgtga gatgttctac ggtcagcgcg tgacccgaat
       61 accgggcgac cctggactac gccggtggct gctgtttgcg atcggcctgc gattgccgcc
      121 cgagaatcgc gagtgggtgc gccacgacct gaccgacgcg ggttggcgga gtcgcgctgc
      181 ggtacggcaa ctcgtgctgg tggctcccgt ttcggccgca ttcgccgcgc tgcccgggcc
      241 gtggtcgttg aaggtaacgc tcatggttct ggtgcttgtc ggcggattgt tgatctcgct
      301 gatgtacgcc gacagcattc gcgcgtcgcg gcttcgccaa cacgatctgc cggtgccaga
      361 agatcgcgac ctgggtcgtc cgaccgactc cttgtaatac ctgcggtcac tcctcgatcg
      421 tgacgagatc gccgatcgca acccggccag gcgccaccgt ggatccgtac accccaaggc
      481 aagcttggcg gtcacgaccg atagcgcgca ggacggccgc gttccgcttg gtcgtctgtg
      541 gatcgacgtt gaccatgatg caccgctggt ctctcttgtc gaggcgcatg cgcaggtcgc
      601 caatgcgcag tacggagccg acccaggcct cctcagggaa gggtgcgccg tcgatcgcct
      661 cgaccaggag attggggcgg aaccgcaatg ggtcgagcgc catcccaacg gtcgcgccga
      721 ggtcggcgag cgttcgggtg ctgatcagcg acaacgggaa ggtgtcgaag actccgcggt
      781 cgtgcttgat cacgcgcgca cccggaccga gctcggcagc cagtatgggg tccgtcacat
      841 cgagttcgtt ccccgccggg gtacgcacca tcgtcggcga tttgtcgggt cggagcggat
      901 cagcgaacga cggttggtac ctccacatga ccggacgttc acgaaccgtc aaccaaggaa
      961 agccactgcg tacgtggccg tcttggatga acgcccagcg gcggtcgcca gccaaaccgt
     1021 gccacgacac gtcgacgtga tccaacacct cggcggccat cgacttgacc gggtaccgaa
     1081 acagcgagac gacccgggcg acgttccggt caaccacggc gcggcctcct tcacgcgaag
     1141 agggagcgcc cttgagccgt agcaccggga ccgagcgtgg cggaccaggt ccgtcgcagc
     1201 gcctctcgac ctcggccgcg ccgccatgca gcgcgatgat ggtgcagcaa cccagcatac
     1261 gaccgcaccc gtcgccgtcc ggaccataat ccctgaaccg agcccgttcc ccagaccgag
     1321 gaccacacgt gaccgagttc cccgatttct ctctcgatgg caaggtcgca ctcgtgacgg
     1381 cgagcggtcg cggattgggt cgcgccatcg cgatcgcctt ggctcacgcc ggcgccgacg
     1441 tcgccctcgg gttgcgcgac cgcgcgtccg caggcgatgt cgtcgaagca atcaccgagc
     1501 tcggacggcg agcagtgccg ctccagatga acatgacgag tctgctcgag atccgcaagg
     1561 caactgccga cgctcacgat gtcttcgggc ggctcgacat cctggtcaac aacgcgggca
     1621 tcgcaccggg tgcaccagca gcgtcggtga ccgaggagga cttcgacgca acgcttgagg
     1681 tcaatctgaa aggaacgttc ttcgcaagtc aggctgcggc gaacatcatg agcgaccaag
     1741 gctccgggcg catcatcaac atcgggtcgc aggccggatt ggtcgcgttg ccaggtgagt
     1801 cgatctactg catgacgaag gcagcgatcg gtcacttgac caagtgcctg gccgttgagt
     1861 ggggcgttct cggcattacg gtcaactgcg tagcgccgac cttcattgag actccaggaa
     1921 ccgagccggc gttgtccgac cctgcgtttc gcgcagacgt ggtcgagcga atcgcggcac
     1981 tccatcgaat cggtcagccc gtcgacgtca ccggtgctgt tgtcttcctc gcctctgacg
     2041 ctgcgtcact catcaccggc acgacgatca ccgtcgatgg cggttggacc gctcgctaaa
     2101 gttcgacgac tcgcacgcgg tcgacgtgca cactatgggc gtggacgacg acgcgctgcc
     2161 agtgccggag gggccagacg ccgtcgccga atggaaggcc aggttgctgg cggtaatcgt
     2221 gttgtggcgg caggcggctg aactggtgtt cgccgacgtg gcaacgacga cggacatccg
     2281 ctggagctgg caggtggcgt tgcacgaaga cgaagacgat tggccggctc gaccgagcga
     2341 tcacgagccc gcggcgtatt ggagtttggt cacccagcac ccgcctggtt gggagccgcc
     2401 gcggcggccg ctgctccttc cggagctctg gctcgaagca gccaaagacg cgacctcgct
     2461 aggcgctggt tacgacctga atcaggcggt cggattcgtc gcgagctgcc tgcaggactg
     2521 ggtcatggac gacattcaac acgcgtggcc ggagtgcccg tggcaccagc atggcgctga
     2581 tctcggtaac ttgggcggcg cgccgacctg ggtctgcccg ttaggcgatc ggctcatagc
     2641 ggaggtggga aagctcggcg gtgctgcgta acgtcaggga cgaccgcgaa gaagcgacac
     2701 ccggaactcg cgtagttcag cacgagccca gccgccggcg aaaacgggat cggcgtcgac
     2761 gatagcttgc gcagcttcct catcctccgc ctcgaatacg acgatgccgg tgttcttcgg
     2821 acccaatgtc ggcccggcca tcaccacgac gccttcagcg agcagtccct tgaggcgctg
     2881 gaaatgctcg tcccagacgg cttcttcctc gtccgtcatg gtggcagcga aattctcgcg
     2941 cggcgggtgg atgaagtaga tccactcggc catggcagga gactgccacg gtcaggttcg
     3001 gtcggcaagg accggctacg cggccgctca gccttagtca accgagccgt tgcgacgtgg
     3061 tcgcgcggtg cgtcggacgc gggttcgtag cgcccgtcgg gcgacgggtc ctagatcctc
     3121 gactacctca acaaggatgc cgagcgtttc caacgacgcg atcgcgttct gcgcgccttc
     3181 gggatcgacg ccctcgaaca actcgatgcc gatgaacgac gcggcgatcc cacgagcgag
     3241 cccttccggg ttggcgactt cactgagtgg gctgcctttg aggagtcgca caagtgtcgt
     3301 ctcgatctca gcgatccaca gcgagagtgc gtgcttggcc gccgcggcga gattggcgtc
     3361 ttgttgcgcg cccgcgagga cctgggcgag cacagtcacg ttgcccgcgc cgcgttcgcg
     3421 gatg
//
LOCUS       DATCBJ010000335         5195 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_198146, whole genome shotgun sequence.
ACCESSION   DATCBJ010000335 DATCBJ010000000
VERSION     DATCBJ010000335.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 5195)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5195)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5195
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_198146"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..826
                     /locus_tag="VII50_06650"
     CDS             <1..826
                     /locus_tag="VII50_06650"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HD-GYP domain-containing protein"
                     /protein_id="HEY5050562.1"
                     /translation="GSIGLIVAVLWVGGVGPVAALLVLAPLVIARWAFEQYSQQHAAY
                     DATVRSLIQAVETKDYYTRGHSERVATASVMIAQQLGMREDRLEMLRYAGMLHDVGKL
                     GVPTKLLQKTGPLDDDEFASIKLHPTRGFEMVNGISFLDEATLGIRHHHERVDGRGYP
                     DGLAGEEIPEFARLIAVADAFDSMTSTRSYRGARSVDAAIRELRRWAGTQFDAGMVEA
                     LVVAVGISGWQASVDATDDVVDPALLVFRDHDDPSSDIAAGFTFETFGIRDEQQVS"
     gene            837..2174
                     /locus_tag="VII50_06655"
     CDS             837..2174
                     /locus_tag="VII50_06655"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HEY5050563.1"
                     /translation="MPASPEREGNGSELAGRIFVALVCIGAVGAAVATGIHGVVRPNV
                     AIGFAVLIAIGELLNVRLPGDRPAAPIASAGALGYAMLFDVGGRPVAQSTAQVIAVTA
                     AGCLAGTLVRAAAGRRPRLTAVARRVVVAAITAAAFRLAAPQRLTMVHFADFKSQLRW
                     VAFLMVAIVALATVLDFVIAAFVRAQDRAAAFGWTLRDEISATAALAAAVGATGVLTA
                     LATPSMQLYALPVFCIPLLLTQFAFRRYAAVRTTYRQTIRSLSRVTEVGGYVEEGHAR
                     RVSQLSIAIGRELGLTENDLLELEYAALLHDIGQLSLIDPIPGGSTLVVAPVERRRVA
                     ELGADVIKTTGVLDRVADIVARQADPYRRHREPPDHTLPIESRIIKAASAYDDLVGAN
                     ATADANADALERLRLGMAYEYDPQVVETLTKVVARRMEHAAAHPSERSRQILA"
     gene            2312..2932
                     /locus_tag="VII50_06660"
     CDS             2312..2932
                     /locus_tag="VII50_06660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HEY5050564.1"
                     /translation="MTASASSYGSSSSFALRRPVLADRLPKSMVRDVALVVGVAAFMS
                     LVAQVSFKLPNNPVPVTGQTFGVLLSAAAVGPLRGLAGQLLYIVIGWAGLPVFADHTH
                     GWSVLSGASGGYFVGFLLAAVLVGWHARHGVDRRVDTLAGSFLLATALIYVPGVWWLA
                     HAENWSYTKAITTGVAPFILGDLLKALAAGALLPAAWKLVGDRTRR"
     gene            2929..4317
                     /locus_tag="VII50_06665"
     CDS             2929..4317
                     /locus_tag="VII50_06665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin carboxylase N-terminal domain-containing
                     protein"
                     /protein_id="HEY5050565.1"
                     /translation="MTSPWTFDSVLVANRGEIARRVIRTVQRLGLKAVAVYSEADAGL
                     PFVKEADEAVLLGASAPAQSYLDVAKVLEAAGQAGAQAIHPGYGFLSENADFARAVAE
                     AGLVWIGPSPEAIIAMGDKVAARNLVGDAGVPVAPGTRAPVADVEAALVAARDVGYPL
                     MVKAAGGGGGIGMSVARDDEELATAFVTARSRAERFFGNPSILLERYVTSARHVEVQI
                     LGLSDGRVVALGERDCSVQRRHQKVAEETPCPVLTPPLRARMIEAAVRAGEAVGYRGA
                     GTVEFLLDPASLASGGEFYFLEMNARLQVEHPITELVTGVDLVEQQLLAASDLPATFD
                     PDAVVRSGHAIELRVYAEDPKRFLPSPGTITRWVEPVGPGVRVDAGYAQGDTVTPFYD
                     PLLAKLCVHGPDRPAALELARTAVASFEIEGLKTNLPFFTELLANEEFVSGVYDTGLI
                     DRMRAPKPAPAS"
     gene            4348..4560
                     /locus_tag="VII50_06670"
     CDS             4348..4560
                     /locus_tag="VII50_06670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-binding carrier protein"
                     /protein_id="HEY5050566.1"
                     /translation="MAEEVRAEMVANVWKIVVAEGDRVEDGDTIAILESMKMEIPVIA
                     EAAGTVSLKVAEGDVVQEGDLIAVIG"
     gene            4623..>5195
                     /locus_tag="VII50_06675"
     CDS             4623..>5195
                     /locus_tag="VII50_06675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HEY5050567.1"
                     /translation="MTGNVTIEPFLRALVESGGSDLHCKVGSPPRVRINGSLRKLQTR
                     DLTAADTEGMVAEVMRKDLVDAFARTNEADFAFSLSGVGRFRVNAFRSRGSAGLVFRR
                     VSVGAIPLEELGLPSILASLALEPRGLVLVTGPTGVGKSTTLAGMIDHINNNREVHIV
                     TVEDPIEVLHFDKLGMINQREVRVDTEDFST"
BASE COUNT          775 a         1733 c         1780 g          907 t
ORIGIN      
        1 cgggtcgatc gggctcatcg tggccgtgct ctgggtgggc ggcgtcggac cggtcgccgc
       61 gttgctcgtg ctggcgccgc tggtcatcgc gcgttgggcc ttcgagcaat acagccaaca
      121 gcacgcggcc tatgacgcca ccgtcaggtc gctcattcag gcggttgaga caaaggacta
      181 ctacacccgc ggtcacagcg agcgcgttgc cacggcctcc gtcatgatcg ctcagcaact
      241 tggtatgcgt gaggatcgcc tcgagatgct ccgctacgcc ggcatgctcc acgacgtcgg
      301 caagctcggc gtcccgacca agctgttgca aaagaccggg ccgctggacg acgacgagtt
      361 cgcgtcgatc aagctgcacc caacgcgagg cttcgagatg gtgaacggga tttcgttcct
      421 cgacgaggcg acgctcggga tccggcatca ccatgagcgg gtcgacggtc gcggctaccc
      481 tgatggactt gccggcgagg agataccgga gttcgcacgg ctgatcgcgg tcgccgacgc
      541 gttcgattcc atgacgtcga cgcgttcgta tcgcggtgcc cgctcggtcg acgccgcaat
      601 ccgcgagttg cgacggtggg ccggtaccca gttcgacgcg ggaatggtcg aagcgctggt
      661 ggtcgcggtc ggcatcagcg gctggcaagc cagcgtcgat gctaccgacg atgtcgtcga
      721 tcccgcgcta ctggtcttcc gcgatcacga cgatccgtcg agcgacattg ctgctgggtt
      781 cacatttgag accttcggca tccgtgatga gcagcaggtc tcgtgacgtg agggccatgc
      841 ccgcgagtcc agagcgcgaa gggaacgggt cggagctcgc cggacggatc ttcgtcgcgc
      901 tcgtatgcat cggtgcggtg ggcgcggcgg tggcgacggg gatccatggc gtggttcgcc
      961 ctaacgtcgc gattggtttc gccgttctga tagcgatcgg cgagctgctg aacgtccggc
     1021 tgccaggtga ccggccggcg gcaccgatcg cttccgcggg tgccctcgga tacgcgatgt
     1081 tgttcgatgt tggcggccga ccggtggccc agtccacggc gcaagtgatt gccgtcaccg
     1141 cggccggctg cctcgcgggg accttggtgc gagccgctgc tggccgccgt ccgcgcctga
     1201 ccgccgttgc gcgacgggtc gtggtggcgg cgataacggc ggcagcgttt cggctcgccg
     1261 caccgcagcg gctgacgatg gtgcatttcg ccgacttcaa gtcccagctg cgctgggtcg
     1321 cattcttgat ggtcgccatc gtcgcgctgg cgacggtgct cgacttcgtg atcgccgcgt
     1381 tcgtgcgggc gcaagaccgc gccgcggcct tcggctggac gctgcgagac gagatcagtg
     1441 cgacggcggc gcttgctgcc gcggtcgggg cgaccggtgt gctgaccgcg cttgccacgc
     1501 cgtccatgca gctctatgcg ttgccggtgt tctgcatccc actgttgctg actcagttcg
     1561 cattccgcag gtacgcggcg gtccgtacga cgtaccggca gacgatccgg tcgctctcgc
     1621 gggtgacgga ggtcggtggc tacgtcgagg aaggtcacgc gcgccgggtc agccagctct
     1681 cgatcgccat cggccgcgaa ctcggcctca ccgagaacga cctgctcgag cttgagtacg
     1741 cggcgcttct gcacgacatc gggcagcttt cgctcatcga tccgatcccc ggcgggtcga
     1801 ccctcgtcgt ggcacccgtg gagcggcgca gggttgccga gctgggtgcc gacgtcatca
     1861 agaccacggg ggtgctcgac cgggtggccg acatcgtcgc ccgccaagcc gacccctacc
     1921 ggcgtcaccg cgagccgccc gaccacacgt tgccgatcga gagccgcatc atcaaggcgg
     1981 ccagcgccta cgacgacctg gtcggcgcca acgccaccgc ggacgcgaac gccgacgcgt
     2041 tggagcggct acgcctcggc atggcgtacg agtacgaccc gcaggtcgtc gagaccctca
     2101 ccaaggtggt cgcccgacgt atggagcatg ccgccgccca cccgagcgaa cgatctcgcc
     2161 aaatcctggc ctgaaacttc gtgcgagttc ccctgtccgg gtccagggca tagaccggta
     2221 ggttctggag gcgatcgtca cccgtcgcct ccaggtggac tgtcgcgtcg gtgacactag
     2281 tcgccgccgt ctcggtgaaa gggaaccgca cgtgaccgct tctgcgtcct cctacgggtc
     2341 gtcaagcagc ttcgccctcc gccgcccggt gctggccgac aggttgccca agagcatggt
     2401 ccgcgacgtt gcgttggtcg tcggcgtcgc cgccttcatg tccttggtgg cccaggtttc
     2461 gttcaagctg ccgaacaatc cggtgcccgt cacgggccag accttcgggg tgttgctgtc
     2521 cgcggccgcc gtgggtccac ttcgcggact ggctgggcag ctgttgtaca tcgtgatcgg
     2581 ctgggccggc ctgccggtct tcgccgacca cacccacggg tggagtgtgc tgagcggcgc
     2641 ttctggcggc tatttcgtcg gcttcctgct cgcggccgtg ctggtcgggt ggcacgcccg
     2701 gcacggcgtc gaccgccggg tcgacacgct cgctggctcg ttcctgctcg ccaccgcgct
     2761 catctacgtc cctggcgtgt ggtggctggc gcatgccgaa aactggtcgt acaccaaggc
     2821 gatcaccaca ggtgtggcgc cgttcatcct gggcgacctg ctgaaggcgc tggctgcagg
     2881 tgcgttgctg ccggccgcct ggaagctggt cggcgaccga acgcgtcgat gacctccccc
     2941 tggaccttcg actcggttct ggtcgcgaac cggggtgaga tcgctcggcg ggtgattcgg
     3001 accgtccagc ggcttggcct caaagccgtc gccgtctatt cggaggccga cgccggcctg
     3061 ccgttcgtca aagaggcgga tgaagccgta ttgctcggtg catccgcacc ggcacagagt
     3121 tacctcgatg tggccaaggt gctcgaggcc gccgggcagg ctggcgccca ggcgatccac
     3181 ccgggctacg gcttcttgtc ggaaaacgcc gactttgccc gcgcagtcgc ggaggcggga
     3241 ctcgtgtgga tcggcccgtc gcccgaagcg atcatcgcga tgggcgacaa agtcgcggcc
     3301 cgcaacttgg tcggcgatgc cggcgtcccc gttgcgcccg gtacccgcgc gcccgtcgcg
     3361 gacgtcgagg ccgcgctcgt cgctgcgcgg gacgtcggct acccgctgat ggtcaaggcc
     3421 gcgggcggcg gtggcggcat cggcatgagc gttgctcgcg acgacgagga gttggcgacc
     3481 gccttcgtca ccgcgcggtc gcgagccgag cggttcttcg gcaacccctc gatcctgctc
     3541 gagcgttacg tcacgagcgc gcggcacgtc gaggtgcaga tcctcgggtt gtcggacggt
     3601 cgggtcgtgg cgttgggcga gcgggactgc tcggtgcaac gccgccacca gaaggtggcc
     3661 gaggagacac cctgcccggt gctcacgccg ccgctgcgcg cgcgcatgat cgaggccgcc
     3721 gtgcgcgccg gcgaggccgt gggttaccgc ggcgccggca cggtggagtt cctgctcgat
     3781 cccgcgagcc tcgcttccgg gggcgagttc tacttcctcg agatgaatgc gcggctgcag
     3841 gtcgagcacc cgattaccga gctggtgacc ggcgtcgacc tagtcgagca gcagctgcta
     3901 gcagcctccg acctgccggc caccttcgat ccggatgccg tggtgcgcag cggccacgcc
     3961 atcgagctgc gggtttacgc cgaggacccg aagcgattcc tgccgagccc gggaacgatc
     4021 acccgttggg tcgagccggt cgggcccggc gtccgcgttg atgccggata cgcgcagggc
     4081 gacaccgtga cgccgttcta cgaccccctg ctggccaagc tctgcgtcca tgggccggat
     4141 cggccggccg cactcgaact ggctaggacc gccgtcgcgt ccttcgagat cgaggggctc
     4201 aagaccaacc tgccgttctt caccgagctg ctggcgaacg aggagttcgt ctcgggcgtc
     4261 tacgacaccg gcttgatcga ccggatgcgg gcgcccaagc ccgcacccgc cagctagcat
     4321 cccgagcact aggagaggag ccacccggtg gccgaggaag tccgcgccga gatggtcgcg
     4381 aacgtctgga agatcgtggt ggccgagggc gaccgggtcg aggacggcga caccatcgcg
     4441 atcctcgagt cgatgaagat ggagatcccg gtcatcgccg aggccgcagg caccgtcagc
     4501 ctgaaggtgg ccgagggcga cgtcgtccaa gaaggtgacc tgatcgccgt catcgggtga
     4561 tctttaacga ttcaggcact ggcatggtca agagagcttc ctgaacggtc gaagcaatca
     4621 ccgtgaccgg taacgtgacg atcgagccct tcttgcgggc cctggtcgaa tctggtggtt
     4681 ccgacctgca ctgcaaggtc ggctcgcctc cgcgcgtacg cattaacggg tccctccgga
     4741 agctccagac gcgagatctc accgcggctg acaccgaggg catggtcgcc gaggtcatgc
     4801 gcaaggacct ggtcgatgcc tttgcccgca caaacgaggc cgacttcgcc ttctcgctgt
     4861 ccggcgtcgg tcgtttccgg gtgaacgcat tccggtccag aggctcggcc ggcctggtct
     4921 tccggcgggt cagcgtcggc gcgatcccgc tcgaggagct tggactcccc tcgatcctgg
     4981 cgtcacttgc cctcgaaccg cggggtctcg tgctggtgac cggcccgacc ggcgtaggca
     5041 agtcgaccac gctcgccggc atgatcgacc acatcaacaa caaccgcgaa gtgcacatcg
     5101 tgacggtcga agacccgatc gaggtcctgc acttcgacaa gctgggcatg atcaaccagc
     5161 gcgaggttcg cgtcgatacc gaagacttct cgacc
//
LOCUS       DATCBJ010000336         4777 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_297484, whole genome shotgun sequence.
ACCESSION   DATCBJ010000336 DATCBJ010000000
VERSION     DATCBJ010000336.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 4777)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4777)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4777
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_297484"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1341)
                     /locus_tag="VII50_06680"
     CDS             complement(<1..1341)
                     /locus_tag="VII50_06680"
                     /inference="COORDINATES: protein motif:HMM:NF009052.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase/fumarate reductase
                     iron-sulfur subunit"
                     /protein_id="HEY5050568.1"
                     /translation="MGYDLKMAVWRGDEDGGELVNYTVAVEEGEVVLDAIHRLQATQA
                     GDLAVRWNCKAGKCGSCSAEVNGRPRLLCMTRLSIFEPAETITITPMRTFPVVRDLVT
                     DVSFNYEKARQVPAFTPPKDLKPGDYRMQQVDVERSQEFRKCIECFLCQNTCHVVRDH
                     EENKPAFAGPRFFIRYAELDMHPLDTVDRKQMLQEEQGLGLCNITKCCTEVCPEHIKI
                     TDNAIIPMKERVVDVRFDPWGKVMRSRREAEQPRTPAAQLRATDAMADSGTSVTAAPS
                     RTVLSAAPAPVKAARAAANAPVAATPVAPATVTPAPVAPTKPAAAPLHTAGSHDLADA
                     DRAVASRTAYMQTLMSLHGVAELLLAGPQRRANRSIKLVVTDDGFATGPLPRPPWRLA
                     VRGTDLVVGDDWLVLPLHGTFADLAANVGVACAPPGDAYPPASGCKPGDRIHIDH"
     gene            complement(1341..3146)
                     /locus_tag="VII50_06685"
     CDS             complement(1341..3146)
                     /locus_tag="VII50_06685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase/succinate dehydrogenase
                     flavoprotein subunit"
                     /protein_id="HEY5050569.1"
                     /translation="MSEIERHTYDVVVIGAGGAGLRAAISAHEAGAKTAVVCKSLLGK
                     AHTVMAEGGIAAAMGNVWEEDNWQVHFRDTMRGGKMLNHWRMAQLHAQEAPDRVWELE
                     SWGALFDRTEEGKISQRDFGGHRYARLAHVGDRTGLEMIRTLQQRAVSLGVDVFMECT
                     VTELLKDGDRIAGAFGYWRESGRFIAFEAPAVVVATGGIGRSFKITTNSWEGTGDGHA
                     LAMQAGAPLVNMEFVQFHPTGMVWPPSVRGLLITESVRGDGGVLRNSEGKRFMFDYIP
                     EFFKAETADTEEEADRWYDDHVNNRRPPELLPRDEVARAINSEVKAGRGTPHGGVYLD
                     IASRRTPEFIQKRLPSMYHQFKELADVDITKEPMETGPTCHYVMGGVEVDPDSAMSIV
                     PGLFAAGECAGGMHGANRLGGNSLSDLVVFGRRAGEHAAAYAKELTGGLPAIADAAVA
                     AAEEAALAPFARDGGQNPYSVQIDLQQTMHDLVGIIRNAGEIEAALVKIEEFKTRIAQ
                     LSVEGHRQYNPSWHLSIDLKNMLLVSECVARAALDRKESRGGHTRDDYPATDPSWGKL
                     NVLCRLDDAGTGVEVFHQDLPVMPDELKALFEESH"
     gene            complement(3148..3990)
                     /locus_tag="VII50_06690"
     CDS             complement(3148..3990)
                     /locus_tag="VII50_06690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5050570.1"
                     /translation="MTVQSKAPESGGGQPPGGRVGRRAAISAKTLRTDRWWIQPVVTF
                     AVLFSFIIYSTWAAFIDRDYFSEPYISPFYSPCLAIKCGTVPGSHGVPHLGVFGHWWF
                     TSPAIIILIVPLGFRMTCYYYRKAYYRSFWLSPPACAVAEPHAKYTGETRFPLIVQNI
                     HRYFFYLGILLNVILTYDAVIAFRNHQGRWGHAGVGTLVLVLNAVFLWSYTASCHSCR
                     SIIGGRLNHFSKHPVRYWLWGQVSRLNRYHMQIAWVSLLWVAFSDVYVRLVASGTLTD
                     LRFF"
     gene            complement(4121..>4777)
                     /locus_tag="VII50_06695"
     CDS             complement(4121..>4777)
                     /locus_tag="VII50_06695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5050571.1"
                     /translation="ATVLGVGLLLGACASPVAKASAPVAPAATHVTDSLPATPAVQAT
                     PPTPAAKAVPVVRVAPVVQVAPVSTPPVAPAVASAPPVATTPPSDTTPPSMPRPSVTT
                     AAAGSAPHFTTPDDAMRYLVAAYNSHDTTAEMHVTTPDARAQLEAERQWVNTFSFAGC
                     QVDGTQGDYICTFDMAAKVATASAGADATGTQMGEITVIVAPASRPGWYMYTSEGCGG
                     "
BASE COUNT          791 a         1520 c         1634 g          832 t
ORIGIN      
        1 gtggtcgatg tgaatacggt cgccgggctt gcagccacta gccgggggat acgcatcgcc
       61 cggtggcgca cacgcaactc ctacattcgc tgcgagatcg gcgaacgttc cgtgcagcgg
      121 caacaccagc cagtcgtcgc cgacaacgag atcggtaccc cggacggcga gccgccacgg
      181 tgggcgcgga agtggcccag tcgcgaagcc gtcgtcggtg acaaccagtt tgatcgagcg
      241 attggcccgg cgctgcgggc cggcgagcaa cagctcggcc acaccgtgca atgacatgag
      301 cgtttgcatg tacgcggtac gggaagcaac cgcgcgatca gcgtcggcca ggtcgtgcga
      361 cccggcagtg tgcaaagggg cggccgcggg cttggtcgga gcaacggggg caggagtcac
      421 tgtggccggc gcaacgggag tggcggcaac tggcgcgttg gcggcggccc tggctgcctt
      481 tacgggtgcc ggagctgcac tcaagacggt ccgcgaaggc gcagcagtga ccgaggtccc
      541 actgtcagcc atcgcgtcgg tcgcgcgcaa ttgcgccgcg ggagtcctcg gctgctcggc
      601 ttcgcggcga ctccgcatga ccttgcccca cggatcgaac ctcacgtcga cgacgcgttc
      661 cttcatcggg atgatcgcgt tgtcggtgat cttgatgtgc tcagggcaca cctcggtgca
      721 gcacttggtg atgttgcata gaccaagtcc ttgctcctct tgcagcatct gcttgcgatc
      781 gaccgtgtcg agcgggtgca tgtcgagctc cgcatagcgg atgaagaacc ggggcccggc
      841 gaacgcaggc ttgttctcct cgtggtcgcg cacaacgtgg caggtgttct ggcacagaaa
      901 gcattcgatg cacttgcgga actcctgcga ccgctcgacg tccacctgtt gcatgcggta
      961 gtcgccgggc ttgagatcct tgggcggagt gaatgccgga acctgccggg ccttctcgta
     1021 gttgaacgag acgtcggtga cgaggtcgcg taccaccggg aaagtgcgca tcggcgtgat
     1081 agtgatggtc tcggcaggct cgaaaatcga gagccgcgtc atgcagagca ggcgtgggcg
     1141 tccgttgacc tcggcgctgc acgagccgca tttgccggcc ttgcagttcc atcggacggc
     1201 gaggtcgccg gcctgcgtgg cctgcagccg atgaatcgcg tcgaggacga cctcgccctc
     1261 ctcgaccgcc acggtgtagt tgacgagttc gccgccgtct tcgtcgcctc gccagacagc
     1321 catcttgagg tcgtagccca tcagtggctc tcctcgaaca acgctttgag ttcgtcgggc
     1381 atgacgggca ggtcttggtg aaacacctca acgccagtgc cggcgtcatc aagcctgcac
     1441 agcacgttga gtttgcccca gctgggatcg gtcgccggat agtcgtcgcg cgtgtgcccg
     1501 ccgcggctct ccttgcgatc gagcgcagca cgcgcgacgc actcggagac cagcagcatg
     1561 ttcttcaggt cgatcgacaa atgccagctg gggttgtact gccgatgacc ctcgaccgag
     1621 agctgcgcga tcctggtttt gaactcttcg atcttgacca gcgcggcctc gatctcgccg
     1681 gcgttgcgga tgatgccgac cagatcgtgc atcgtctgct gcaagtcgat ctggacgctg
     1741 tacgggttct gcccgccgtc ccgcgcgaat ggcgcgagtg cggcttcctc tgcggcggcg
     1801 accgccgcgt cggctattgc cgggagccca ccggtcagct ctttcgcgta ggcggcggca
     1861 tgttcaccgg cccgacggcc aaacacgacc aggtcgctca atgagttgcc gccgaggcgg
     1921 ttagcgccgt gcatcccgcc cgcacactcc ccggccgcga acagcccggg cacgatcgac
     1981 atcgcggagt cgggatcaac ctcgaccccg cccatcacgt agtgacaggt cgggccggtc
     2041 tccatcggct ctttggtgat gtcgacgtcg gccagctcct tgaactggtg atacatcgac
     2101 ggtagacgct tttggatgaa ctccggtgtg cggcgcgacg caatgtcgag gtagaccccg
     2161 ccatgtggtg tgccgcggcc ggctttcacc tcggagttga tggcgcgggc gacctcgtcg
     2221 cgaggcaaca gctcgggcgg ccgccggttg ttgacgtggt cgtcgtacca ccggtcggcc
     2281 tcttcttcgg tatcggcggt ctccgctttg aagaactccg gaatgtaatc gaacatgaag
     2341 cgcttgcctt cggagtttcg gaggacgccg ccgtcgcctc gcaccgattc ggtgatgagc
     2401 aggccgcgga ccgacggcgg ccacaccatg ccggtcgggt ggaactgcac gaactccatg
     2461 ttgaccagcg gagcgccagc ttgcatcgct agcgcgtggc catcgccggt gccttcccag
     2521 gagttcgttg tgatcttgaa tgatctcccg atcccaccgg tggccaccac tacagctggc
     2581 gcttcgaagg cgatgaagcg gccggactcg cgccaatatc cgaaggcgcc ggcgatgcgg
     2641 tcaccgtctt tcagcagttc ggtgacggtg cattccatga agacgtcgac gccgagcgag
     2701 acggcgcgct gttggagggt gcggatcatc tccaggcccg tgcggtcgcc gacgtgcgcg
     2761 aggcgtgcgt agcggtggcc gccgaaatcg cgttgggaga tcttgccttc ctccgtgcgg
     2821 tcgaacaggg ctccccaact ctcgagttcc cacacccggt ctggcgcctc ctgtgcatgc
     2881 agctgcgcca tccgccagtg gttgagcatc ttgccgccgc gcatggtgtc gcggaagtgc
     2941 acctgccagt tgtcttcttc ccacacattg cccatggcag cggcgattcc gccctcggcc
     3001 atcactgtgt gcgccttgcc cagcaacgat ttgcagacga cggcggtctt tgcgccagcc
     3061 tcgtgcgcgc tgatggctgc gcgaagcccg gcgccgccag ccccgatcac gacgacgtcg
     3121 taggtgtgcc gctcgatctc gctcatctca gaagaacctc aggtcggtga gagtgcccga
     3181 tgccacaagc cgcacgtaca cgtcggagaa tgccacccat agcagcgaaa cccacgcgat
     3241 ctgcatgtgg taacgattga gccggctgac ctgaccccac aaccaatagc gcaccgggtg
     3301 cttggagaag tggttgaggc ggccgccgat gatgctgcgg cacgaatggc aggacgcggt
     3361 gtacgaccac aagaacaccg cgttgagtac cagtacgagc gtgccgacac cggcgtggcc
     3421 ccagcggccc tgatggttgc ggaacgcaat aaccgcgtcg taggtcaaga tgacgttgag
     3481 caggatcccg aggtagaaga agtaccggtg gatgttctgc acgatcagcg ggaagcgcgt
     3541 ctcgccggtg tacttcgcgt gcggctccgc aacggcgcag gccggcggcg acagccagaa
     3601 cgatcggtag taggccttgc ggtagtagta gcaggtcatc cggaagccga gcggcacgat
     3661 caggatgatg atcgcgggcg aggtgaacca ccaatgcccg aacacgccca gatgcgggac
     3721 gccgtgtgag ccgggcaccg tgccgcactt gatcgcgagg caaggggagt agaacggcga
     3781 gatgtagggc tcggagaagt agtcgcggtc gatgaacgcg gcccacgtcg agtagatgat
     3841 gaagctgaac aggacggcga aggtgacgac cggctggatc caccaacggt cggtgcgcag
     3901 ggtcttcgca ctgatggccg ctcggcggcc gacgcggccg cccgggggtt gcccgccacc
     3961 agactccgga gctttgctct ggacggtcat cggccccgcc ttctcgtgcc cgcgtcgtta
     4021 cgggtcatac gggagcacaa cctacgcccg aggctggatc cgctcccagc ggaggcggtg
     4081 tggcatccgt cactcagcag tcgccgggcg ggcccgaagc tcagccgccg cagccctcgc
     4141 tcgtgtacat gtaccagccg ggtcgggagg cgggcgcgac gatgacggtg atctcgccca
     4201 tctgggtgcc tgtggcgtcg gcccccgccg acgccgttgc gaccttcgcc gccatgtcga
     4261 acgtgcagat gtagtcgcct tgggtgccgt cgacctggca accggcgaag ctgaacgtgt
     4321 tcacccactg ccgctcagcc tcgagctggg cacgcgcatc gggcgttgtg acgtgcatct
     4381 ccgctgtggt gtcgtggctg ttgtaggccg cgaccaggta gcgcatggcg tcgtcggggg
     4441 tcgtgaagtg aggggccgaa ccggcggcgg cagtcgtcac ggacggcctc ggcatggacg
     4501 gcggggtggt gtccgacggc ggggtggtgg cgaccggcgg cgcgctcgcc acggctggcg
     4561 ccaccggagg tgtgctgacg ggcgcgacct gaaccaccgg cgcgacccgg acaaccggca
     4621 cggccttggc cgcaggcgtc ggcggcgtgg cctgcaccgc aggcgtcgca ggaaggctgt
     4681 cggtgacgtg tgtcgcggcc ggcgcgacgg gagccgaggc cttggccacc ggactggcgc
     4741 aggctcccag caggagtccg acgccgagta cggttgc
//
LOCUS       DATCBJ010000337         4751 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_308606, whole genome shotgun sequence.
ACCESSION   DATCBJ010000337 DATCBJ010000000
VERSION     DATCBJ010000337.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 4751)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4751)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4751
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_308606"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1005)
                     /locus_tag="VII50_06700"
     CDS             complement(<1..1005)
                     /locus_tag="VII50_06700"
                     /inference="COORDINATES: protein motif:HMM:NF013998.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HEY5050572.1"
                     /translation="MRAALSGLTTRGRSFLAAGIAACLCGLVLREMDLLRVGIFLCVL
                     PIATAIVVARTRYRLSCVRRIEPQRVPVGETAQVVLRVENLSRVATGILLLEDRLPYS
                     LGGRPRFVVNRIESLGVREVSYPVRSDVRGRFVVGPLSLRFADLFGLVELTRSFTTTD
                     ALVVTPTVVPLPAIRLGGDWAGGGDSRARSISTMGEDDVTTREYRHGDDLRRVHWRST
                     ARYGELMVRREEQPWQNRGVLLLDSRVTAHRGEGPGSSFEWAVSAIASIGSHLARDGF
                     GLRFTTESGSEVSGYSADAMAGTPFEAVLLDTLALVSMSGAHSLTAGLAGIRRGGGGG
                     L"
     gene            complement(1005..2003)
                     /locus_tag="VII50_06705"
     CDS             complement(1005..2003)
                     /locus_tag="VII50_06705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEY5050573.1"
                     /translation="MAAFPGTEGGDLKEVAETGRRICAAVEQVIEGKPDVVQLAVTVL
                     FAEGHLLIEDVPGVGKTMLAKALAKAIDCSVRRIQFTPDLLPSDITGVSVFSQDTREF
                     EFKPGPVFANIVVGDEINRASPKTQSALLESMEERQATVDGTTYRLAAPFMVVATQNP
                     IEMEGTYPLPEAQRDRFTARLSMGYPSATAELEMLDSHGSHDPLDDLRPVADAVEVAN
                     AIELIRTVYVAPDVRRYTVDLVAATRASLELRLGASPRATLQLLRAARAAAALDDRDY
                     VLPDDVRMLTQPVLTHRLLITAEAQISRRTASDILSDIVARVAVPSSTQRSRATPH"
     gene            2332..2763
                     /gene="mraZ"
                     /locus_tag="VII50_06710"
     CDS             2332..2763
                     /gene="mraZ"
                     /locus_tag="VII50_06710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005961682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0051302 - regulation of cell division
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HEY5050574.1"
                     /translation="MFLGTHAPRLDEKGRLFLPAKFRDELAAGLVITKGQERCLYVFP
                     AVEFGRLTEALRTSPVTHKGVRDYSRVFFASASDDIPDKQGRITIPSALREYAGLSRD
                     CAVIGANTRVEIWDAAAWETYLAAQEPVFSELGEEVLPGVL"
     gene            complement(2783..2971)
                     /locus_tag="VII50_06715"
     CDS             complement(2783..2971)
                     /locus_tag="VII50_06715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5050575.1"
                     /translation="MPIPRDVVHLAFSTLRVLCGGAKWSASCVGVPSRSPEVPVRLAP
                     GKVCQESERPEASKRVAL"
     gene            2972..3907
                     /gene="rsmH"
                     /locus_tag="VII50_06720"
     CDS             2972..3907
                     /gene="rsmH"
                     /locus_tag="VII50_06720"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009080392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HEY5050576.1"
                     /translation="MPVLLGRVLELLAPVLTAPGAVVVDATLGMGGHAEALLVAHPQA
                     RLIGIDRDTDALALATARLAPFETRITLVHAVYDQLPVVLEELGLSAVDGILFDLGVS
                     SLQLDETERGFSYAHDAPLDMRMDRTTGKTAADVVNTYSAAEIAQVLRVYGEERFASR
                     IANAIVQERTRQPLVSASRLAELVRDSIPAATRRTGGHPAKRTFQALRIEVNAELTAL
                     ERAVPAAVAAVRVGGRVVVLSYQSLEDRLVKQVFAAGAASTSPSQLPVELPDHAPRLR
                     LLTRGADQASATEVAANPRAASVRLRAIERIRQAA"
     gene            complement(4215..>4751)
                     /locus_tag="VII50_06725"
     CDS             complement(4215..>4751)
                     /locus_tag="VII50_06725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5050577.1"
                     /translation="AGCPETVHACPPPVGFAVGPAVGVGFAVGFAVGFVVGFAVGLAV
                     GFAVGLAVGAAVGLPEADGLRLADGDAPGDADADALADDVADGEPEADGDVDPDGAAD
                     ADTLALADADACDVGDGFGAGGGAGRQAFVAAPSTTPSDDRVKTGFGAATMPSATARA
                     AKACGTPWSATDWLSACT"
BASE COUNT          785 a         1604 c         1608 g          754 t
ORIGIN      
        1 caacccgccg ccaccaccac gtcgaatgcc ggcgagtccc gcggtgagcg agtgcgcacc
       61 cgacatcgag accagcgcga gggtgtcgag cagcacggcc tcgaagggtg tgccggccat
      121 cgcgtccgcc gaatatcccg agacctcgct accggactcg gtggtgaagc gcagtccgaa
      181 tccgtcgcgg gccaaatggg agccgatcga tgcgatcgcg ctgaccgccc attcgaatga
      241 cgagccgggg ccttcaccac gatgggccgt caccctgctg tcgagcagca gcacgccccg
      301 gttctgccac ggctgttcct cgcggcggac catgagttcg ccgtatcggg cggtcgatcg
      361 ccagtgcacg cggcgcaggt cgtcgccgtg gcggtactcg cgggtggtga cgtcgtcctc
      421 gcccatcgtc gatatcgaac gtgctctgct gtcgccgccc ccggcccagt cgccgccgag
      481 tcgaatcgcc ggcagcggca cgacggtggg cgtgacgacg agggcgtcgg tcgtcgtgaa
      541 cgatcgggtc agctcgacga gtccgaagag atcggcgaaa cgcaaggaca agggacccac
      601 gacgaagcgg ccgcgcacgt cactgcgtac cgggtaggaa acctcgcgca caccgagcga
      661 ttcgatcctg tttaccacga atcgagggcg gccaccgagg ctgtacggca agcgatcctc
      721 aaggagcagg atgccggtcg cgacccgcga caagttctcc acgcgcagga ctacctgtgc
      781 cgtctcaccg acgggcacac gttgcggctc gatgcggcga acgcacgaaa gccggtagcg
      841 ggtgcgcgcc acaacgatcg ccgtcgcgat cggtagcaca cacaagaaga tgccgacgcg
      901 cagcagatcc atctcgcgaa ggacgagacc gcacagacac gcagcgatgc cggccgcgag
      961 gaaacttctg ccgcgggtgg tgagtccgga aagcgccgcc cgcatcagtg tggcgttgct
     1021 cgggaacgct gggtggacga cggaactgcg actcttgcca cgatgtcgga caagatgtcc
     1081 gacgcggtgc gtcgcgagat ctgcgcttcg gcggtgatga gcagccggtg ggtcaaaacg
     1141 ggctgcgtga gcatgcggac gtcgtcgggg agcacgtagt cgcggtcatc gagtgcagcc
     1201 gcagcgcggg cggcccgtag cagctgcagg gtcgcacgcg gcgacgcccc gagccgcagt
     1261 tcaagcgatg cgcgggtggc cgcgacgagg tcgactgtgt aacgacggac gtcgggcgcg
     1321 acatagaccg tgcggatcag ttcgatggca ttcgcgactt cgacagcgtc ggcgacgggg
     1381 cgcaggtcgt cgagcggatc gtggctgccg tgggaatcga gcatctccag ttcggcggtg
     1441 gcggacgggt agcccatcga cagccgcgcg gtgaaacggt cacgctgcgc ttccggtagc
     1501 gggtaggtgc cctccatctc gatcgggttc tgggtggcaa ccaccatgaa cggcgcagca
     1561 agtcggtagg tcgtgccgtc gacagtcgcc tgccgttctt ccatgctctc gaggagtgct
     1621 gactgcgtct ttggcgacgc gcgattgatc tcgtcgccga cgacgatgtt ggcgaacact
     1681 ggaccgggct tgaactcgaa ttcgcgagtg tcctggctga agaccgacac acctgtgatg
     1741 tcgctgggga gcaagtcggg ggtgaactgg atccggcgca ccgaacagtc gatggctttc
     1801 gccagcgcct tggccaacat cgtcttgccg acgccgggca cgtcctcgat gagtaggtga
     1861 ccttcggcga acagcaccgt gacggcgagc tgcacgacgt ccggcttgcc ttcgatcacc
     1921 tgctcgacgg cagcgcagat acgcctaccg gtttctgcga cctctttgag atcgcccccc
     1981 tctgtccccg gaaacgctgc cacccgtcct ccttgctcgt gtccggcggt cgagcctgct
     2041 cgcgaactcg cggtcgactt tgctcgcagc actcgcttcg agcttgcgcg cagtgtcact
     2101 gagtctctca ggtcaagagc gcgcaaacga gtgatccccg tactcgaacg gatcacaggg
     2161 cgcatcggtt cgcctcgcgg ctctggtgat tacggcggca aagggagtga tcgcagggat
     2221 cgatggccct aggttacttg aaagtggagg agtgtggagt atggtggcgc gaaatggaga
     2281 gcaagggtag gggccggcgg gcggccgacg tcgggcgagc ggaggtggcg ggtgtttctc
     2341 ggaacccacg cccctcgact cgatgagaag ggtcgactct tcttgccggc gaaattccgt
     2401 gacgagctcg cggccggcct ggtgatcact aagggccagg agcgttgcct ctatgtcttt
     2461 cctgccgtgg agttcggccg gctgaccgag gcgcttcgaa cgtcaccggt gacccacaaa
     2521 ggggtccgcg actactcgcg ggtctttttc gcgagcgcat cggacgacat ccccgacaag
     2581 caaggtcgca tcacgatccc gtccgcactc cgcgaatacg cgggcctcag ccgtgactgc
     2641 gcggtcatcg gcgcaaatac cagggtcgaa atctgggacg ccgcggcctg ggagacctac
     2701 ctcgccgcac aagagccggt cttctccgaa ctcggtgagg aggtgctgcc aggggtcctt
     2761 tgatcggacc cgcctctgat cactacagcg ccacgcgctt cgaggcctcc ggccgctcgc
     2821 tctcctggca caccttcccc ggcgccaggc gaacgggaac ctccggtgag cggctgggaa
     2881 ccccgacgca cgaggcgctc catttcgcac caccgcacaa cacccgaagg gtcgagaacg
     2941 ccaaatggac gacgtcacga ggtatcggca cgtgccggtc cttctcggtc gggtgctcga
     3001 gctgctcgcg ccggtgctga ccgcgcccgg tgcggtcgtg gtcgatgcga cgctcggcat
     3061 gggcggccac gcggaggcgc tcctggtcgc ccatccgcag gcccgactca tcgggatcga
     3121 ccgggacacc gacgcgctcg cgctcgcgac cgcacggctc gcccccttcg agacgcggat
     3181 caccctcgtg cacgctgtgt atgaccaact ccctgtcgtg ctcgaggagc ttgggctatc
     3241 ggcggtcgac gggatcttgt tcgaccttgg ggtctcctcg ctgcagctcg acgagaccga
     3301 gcgcgggttc tcctacgccc acgacgcgcc gctcgacatg cggatggatc gaaccacggg
     3361 caagaccgcc gcagacgtcg tcaataccta ttcggccgcc gagatcgccc aggtgttgcg
     3421 cgtgtatggc gaggagcgtt tcgcgtcgcg gattgccaac gcgatcgtcc aagagcggac
     3481 ccgccaacct ctggtctcgg cgtcgcgctt ggcggagttg gtacgtgact cgatccccgc
     3541 cgcgacccgg cgtaccggtg gtcacccggc gaagcgcacg ttccaggcgt tgcgcatcga
     3601 ggtcaacgcc gagctcacgg cgctcgagcg ggcggtgccg gccgcggtcg ctgccgtccg
     3661 cgtcggcgga cgagtcgtgg tgttgtcgta tcagtccctc gaagaccgtc tggtgaagca
     3721 ggtcttcgcc gctggtgccg ccagcacatc gccgtcccaa ctgcctgtcg agcttcctga
     3781 ccacgccccg cgcctacggc tgctcacccg cggagccgat caagccagcg cgaccgaggt
     3841 ggcggccaat ccgcgggccg cgtccgtgcg cctgcgcgcg atcgagcgca tcaggcaggc
     3901 cgcgtgagcg cgcttccatt cgttggagtg acggtgcctg aagtgacggc acccgatgcc
     3961 gccgtcgatg gggttctgat cgctgcggcg acgaccgcgc ccgttagcgc aacgccatcg
     4021 agtgcgctgg tcaccgggtc atcgacgctg cgaatcgtgc gtccgcaaaa cgcgcggccg
     4081 cgagcccctt tcgtgctgct ggtctcgggg ctgctcgcgg ccggcctggt cggggtgttg
     4141 ctgatcaaca cgaagcttgc ccagggctcg ttcgccgtgg accagctcca gcaccaacag
     4201 tcgacctacg ccgatcaggt gcaggcactg agccagtccg tcgccgacca gggagttccg
     4261 caggccttgg cggcacgggc ggtcgcgctc ggcatggttg cggcgccaaa cccggtcttc
     4321 acgcgatcgt cggacggcgt ggtgctgggc gccgcgacga aggcctggcg tccggcgccc
     4381 ccacccgctc cgaagccgtc gcccacatcg caggcgtcgg cgtcggccag cgcgagcgtg
     4441 tctgcgtcgg cagcaccgtc ggggtcgacg tcgccgtcgg cctccggctc gccgtccgcg
     4501 acatcgtcgg ccaaggcatc cgcgtccgcg tcgccagggg catcgccatc ggctaaccga
     4561 agtccatcgg cctcgggcaa gccaaccgcc gcgccaactg ccaatccgac ggcgaagcca
     4621 accgccaatc cgacggcgaa gcccaccacg aagccgaccg cgaagccgac cgcgaagccc
     4681 acgccgactg ctggcccaac cgcgaaaccc accggcgggg ggcacgcgtg aaccgtctcg
     4741 gggcacccgg c
//
LOCUS       DATCBJ010000338         3085 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_486573, whole genome shotgun sequence.
ACCESSION   DATCBJ010000338 DATCBJ010000000
VERSION     DATCBJ010000338.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 3085)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3085)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3085
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_486573"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..223
                     /locus_tag="VII50_06730"
     CDS             <1..223
                     /locus_tag="VII50_06730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5050578.1"
                     /translation="TAQIEDGVVLADSELRGKIASRYPDAWSRIQQRRSMVTKVFGIE
                     LHDEVLPLSNAAAYFPPLWLSPQLAMVRI"
     gene            735..2804
                     /locus_tag="VII50_06735"
     CDS             735..2804
                     /locus_tag="VII50_06735"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEY5050579.1"
                     /translation="MTIRRKLDTYIGIAVLVAALLVAFSVTNAVRHGLTIALLPVLVL
                     ASVLIIGELRPIRISHGDGSVDEVTISSSFSLALVLTGPLIVALGAQTIATLLDDVRQ
                     RKDLRRIVFNQAQYIITLSASRLAYSATTGRGFFEDTRDLRPRDIVGALAAAVVFFVV
                     NHMVTTIVIALSLGENPIQRMPMDLRYHLSTSGVLLALAPVLVIAVDFSAFALPLLVL
                     PIAAVHKSAKLAIQREEESLHDSLTGLPNRTLFHRTASTMLARASTTGSSTALMIVDL
                     DHFKEINDTLGHHIGDQVIEAAADRLSTALGANAMVCRLGGDEFAVLVPDIEPTAVLI
                     VAHIALRTLAAPLTADGVRIDVGASIGVAVAPEHGLDVSTLLRRADVALYAAKEERNA
                     VRLYSLDDDRNTVERLELLGDLRGAIEHGEVIVHYQPKISALTGGVIGVEALARWQHP
                     ERGLIMPDDFINLAEHTGLLSLLTASVLETALADLRRWRTVEPTLTVAVNVAPAQLND
                     REFPESVWVALHRAGVPAAALTLEVTENGVMASSGRVRQVLDDLRRMGVQLSIDDFGS
                     GATSLSYLGRLPLNELKIDKAFVIGLADPVNHAIVRSTIELGHNLGMQVVAEGVETVD
                     VWHELQVLGCDVLQGYVIARPLTQIAMTSSLSSPRAVAPIAYRARLADVSDVALGAVS
                     AFPAPQR"
     gene            2826..>3085
                     /locus_tag="VII50_06740"
     CDS             2826..>3085
                     /locus_tag="VII50_06740"
                     /inference="COORDINATES: protein motif:HMM:NF036410.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5317 family protein"
                     /protein_id="HEY5050580.1"
                     /translation="MALLAGLLVATVPLFGGRLRALAHVRIQSFWLIAGALALQVLAI
                     SVVPSWPRGLLVGAHLVSYLMAGWFVWLNRRVSGLPIVALGGA"
BASE COUNT          470 a          984 c         1002 g          629 t
ORIGIN      
        1 cacggcacag attgaggacg gcgtggtctt ggcggactcc gagctgcgag gaaagatcgc
       61 ctcgcggtac ccggacgcgt ggagccggat tcagcaacgg cggtcgatgg tcacgaaagt
      121 cttcgggatc gagctgcacg atgaggtgct gccgctatcg aacgcggccg cctatttccc
      181 gccgttgtgg ctgtcgcccc agctggcgat ggtccggatc tgacaaatcc gcctaaattg
      241 cctggccgcc tgcgccgttt ccacccgttc gattcctgga gtcggcatct gcgctctgtc
      301 aatcggcggt cggctatccg cgactagcaa acgggtgcaa agagttccct caggtgggcg
      361 atgggttgcg gaacgcccgc tgccgatcac tcttacatca gggtggctgc cgtggaattg
      421 ccgcggcgcc tggcttgccg tggggaggtt cgtatgtcgc gttcacgttt gttcggtcgt
      481 gtcctggggt ctgctacgag tttggtagcc ctcgttgccg tcgtcggtgc tggctggaag
      541 tggtagttcg ctgctgttgc cgctaacaca gtcgtagccg ctaaacggag aggagcctaa
      601 gtcgtgaagg cacagctcat gacaagcgtt gtcgacgccg atggagccct cgggggagtc
      661 ttggtcgccg gccacaagtg gcgctagatc gcttgcgcta gctagatctt gcaggagagg
      721 agggcagcac ccatatgacc attcgccgca aactcgatac ctacattggc atcgcggttc
      781 tggttgctgc cctcctcgtc gctttttcgg tgaccaacgc cgtccggcac ggcttgacca
      841 ttgcgctgct tcccgttttg gtgctggcgt ccgtgctgat catcggtgag cttcgcccga
      901 tccggatctc tcacggtgac ggcagcgtcg atgaggtcac catctccagc tcgttcagcc
      961 tggccctcgt gttgaccggc ccactcatcg tcgcgctcgg cgcgcagacg atcgcgacgc
     1021 tgctcgacga cgtacgccag cgcaaggatc tgcgcaggat cgtcttcaac caggcgcagt
     1081 acatcatcac cttgagtgcg tcgcgcctcg cgtactcggc gaccacgggt cgcggcttct
     1141 tcgaagacac tcgcgatttg cggccacgcg acatcgtcgg cgcgctcgcc gcggcggtcg
     1201 tgttctttgt ggtcaaccat atggtcacca cgatcgtcat cgctttgtcg ttgggtgaga
     1261 acccgatcca gcggatgccg atggacttgc gctatcactt gtcgacgtcc ggcgtgttgc
     1321 tcgcgttggc gccggttctg gtgatcgccg tggacttctc cgccttcgcg ctgccgctgc
     1381 tcgtgctgcc gatcgctgcc gtgcacaaga gcgccaagct cgccatccag cgcgaggaag
     1441 agtctttgca cgactcgttg accggcttgc cgaaccggac cttgttccac cgaacggcga
     1501 gcacgatgtt ggctcgagca tcgacaaccg gatcgtcaac ggccttgatg atcgtcgacc
     1561 tcgaccactt caaagagatc aacgacacgc tcgggcacca catcggcgac caggtgatcg
     1621 aggcggccgc agatcggctg tcgacggcgc tgggcgccaa cgcgatggtg tgccgcctcg
     1681 gtggcgacga gttcgcggtg ctcgtgccgg atatcgagcc aaccgccgtc ctgatcgttg
     1741 cgcacatcgc gttgcggacg ctggccgcgc cactaacggc ggacggcgtt cgcatcgatg
     1801 tcggagcgag catcggcgtc gcggtggcac ccgagcacgg gctcgacgtg tcgaccctgc
     1861 tccgacgcgc agacgtcgcc ctttacgccg ccaaggaaga acgcaacgcg gtccgcctgt
     1921 attcgctgga cgacgaccgc aacacggtcg agcggctgga gctcctcgga gatctgcgcg
     1981 gcgcgatcga gcacggcgag gtcatcgtgc actaccagcc aaagatttcc gcactgaccg
     2041 gcggcgtcat cggtgtcgag gccttggccc gctggcagca tcccgagcgc ggcctcatca
     2101 tgcccgacga cttcatcaac ctcgccgagc acaccgggct gctgtcgttg ttgaccgcgt
     2161 ccgtcctcga gacggcgctc gcggacctgc ggcgttggcg cacagtcgag ccgacattga
     2221 cggttgcggt caacgtggcg ccggcgcaac tgaacgaccg cgagtttccc gaatctgtct
     2281 gggtcgccct gcatcgagcc ggcgtacccg ctgcggcgct cacgctcgag gtcaccgaaa
     2341 acggggtcat ggcgtcaagt ggccgggttc ggcaagtact cgacgacctg cggcggatgg
     2401 gtgtgcagct atcgatcgac gacttcggaa gcggcgcgac gtcgctttcc taccttggcc
     2461 ggctgccgtt gaacgagctg aagatcgaca aggcgttcgt gatcggactt gccgatccgg
     2521 tgaaccacgc catcgtgcga tcgacgatcg agctcggaca caacctcggg atgcaggtcg
     2581 tcgcggaggg cgtggagacg gtcgacgtgt ggcacgaact ccaggtactc ggttgtgacg
     2641 ttctccaggg ttatgtcatt gcgcggccgc tgacccagat tgctatgacg tcgtcactgt
     2701 cgagccctcg ggcggttgct ccgatcgcgt accgcgcccg gcttgccgac gtatccgacg
     2761 tagccctcgg tgctgtctcg gccttcccgg ctccgcagcg ctgaccgggg aggctcaccg
     2821 tgctgatggc cttgctggcc gggttgctcg tcgcgacagt tccgctgttt ggtggccggc
     2881 tgcgggccct cgcgcacgtg cgcatccagt ctttttggct gatcgctggc gcgctcgcct
     2941 tgcaggttct cgcgatcagc gtcgtgccga gctggccgcg cggattgctc gtcggtgccc
     3001 accttgtcag ctacctgatg gctggctggt ttgtctggct taatcggcgc gttagcggac
     3061 tgccgatcgt cgccctcggt ggcgc
//
LOCUS       DATCBJ010000339         2123 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_399043, whole genome shotgun sequence.
ACCESSION   DATCBJ010000339 DATCBJ010000000
VERSION     DATCBJ010000339.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2123)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2123)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2123
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_399043"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1154)
                     /locus_tag="VII50_06745"
     CDS             complement(<1..1154)
                     /locus_tag="VII50_06745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HEY5050581.1"
                     /translation="MNATSLILLVLGLALGAAIGFLHAKSRSSATIASLSATLDETRS
                     SATQQLEALRAYAAEQLQLANLTQEKMRETFAALSSDALHKNSTAFIERADELIKRVT
                     AESTGDLEKRQQAIELLVSPLKETLTNVEKQMKDVEKERTDAYAALRTQVAQMGKTSE
                     KLQQETSQLVAALRAPQTRGAWGEHQLRQVVDFAGMIEHCDFGEQQSVNTPDGMLRPD
                     LIVNLAGGKHVVVDAKVSLIGFLDAMAVDDAAADAATTAATRAHHLKRHARHLRDHID
                     QLGAKAYWDLLPNTPEFVVMFIPAETFLNAALEQDPALLQHAFEKNVVIATPATLIAL
                     LRTVGYTWRQEALAANAHDVLTLGRELYARLSTMGRHVGTLGTRLNSAVQ"
     gene            1225..1779
                     /locus_tag="VII50_06750"
     CDS             1225..1779
                     /locus_tag="VII50_06750"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY5050582.1"
                     /translation="MPIEMVRGPVLIRSWHQSDAAGLARVIFENLDHLRPWMPWALEL
                     PKNRTKVRAAMGAFLLEAVTKPDELVGLFSGGQIAGGSGLHPRIGPGGYEIGYWVRKD
                     LTRRGIATAATNALTEHAFTRHDIDRVEIRHDRANLASECIPRRLGYALVGEEPSVIA
                     APEQSGVHLIWRVTRDEWTARQRP"
     gene            1791..>2123
                     /locus_tag="VII50_06755"
     CDS             1791..>2123
                     /locus_tag="VII50_06755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003884384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HEY5050583.1"
                     /translation="MALSIGIVGLPNVGKSTLFNALTKNNVLAANYPFATIEPNVGVV
                     GVPDSRLNALAALYSSVKIVPATVSFVDIAGLVKGASEGQGLGNKFLANIRESDAICQ
                     VTRVFTDPD"
BASE COUNT          349 a          657 c          722 g          395 t
ORIGIN      
        1 ctctgcaccg cggagttgag gcgggtgccg agcgtcccga cgtggcggcc catggtcgag
       61 agcctcgcgt acagctcgcg acccagggtg aggacatcgt gggcgttggc ggcgagtgcc
      121 tcctgccgcc aggtgtaacc gacggtacgc agcagcgcga tgagggtcgc cggtgttgcg
      181 atgaccacgt tcttctcgaa cgcgtgctgc aacagtgcgg ggtcctgctc gagtgccgcg
      241 ttaaggaacg tctctgccgg gatgaacatc acgacgaact caggggtgtt cggcagcaga
      301 tcccagtacg ccttcgcgcc gagttgatcg atgtggtcgc gcaggtggcg cgcgtgacgc
      361 ttcaagtgat gcgcgcgcgt tgccgcggtc gtcgcagcgt cagcagccgc atcgtcgacg
      421 gccatcgcgt cgaggaagcc gatgagtgag accttcgcgt cgacgacgac atgcttgccg
      481 ccggccaggt tgacgatgag gtcgggacgc agcatgccgt cgggagtgtt cacggactgc
      541 tgctcaccga agtcgcagtg ttcgatcatg ccggcgaagt cgacgacctg gcgaagctgg
      601 tgttcacccc aggcgccgcg ggtctgcggc gcgcgcaagg cggcgacgag ctgcgacgtc
      661 tcttgctgca acttctccga cgtcttgccc atctgtgcga cctgtgtgcg aagagcggcg
      721 tacgcgtcgg tgcgctcctt ctcgacgtcc ttcatctgct tttcgacgtt tgtgagggtc
      781 tctttcagcg ggctgaccag gagttcgatc gcctgctgcc gcttctcaag atcgccggtc
      841 gactccgcgg tgacccgctt gatcagctcg tcagcgcgct cgataaaggc tgtgctgttc
      901 ttgtgcagcg cgtcggagga cagcgcggcg aaggtctcgc gcatcttctc ttgcgtgaga
      961 ttcgccaatt ggagctgctc agccgcgtac gcacgcagcg cttcgagctg ctgcgtggcg
     1021 ctggaccggg tctcatcgag agtggcggac agggaagcga tggtcgcgct cgaccgtgac
     1081 ttggcgtgca ggaagccgat cgcggcgccc agcgcgaggc cgagtacgag caaaatgagc
     1141 gaagttgcgt tcatgtctcg atcgtggcag gggcgtccga caaaacttcg ctatgccacg
     1201 ccggacgccc taggcttgag caatatgccg atcgagatgg tccgtggacc cgtgctgatc
     1261 cgctcgtggc atcagtcgga cgccgcgggg ctggcgcggg tgatcttcga gaaccttgat
     1321 cacctccggc catggatgcc ctgggcactt gagctgccga agaaccggac gaaagtccgc
     1381 gcggcgatgg gcgccttcct gctcgaggcg gtcacgaagc cggatgagtt ggttggcctg
     1441 ttctccggcg gccagatcgc gggcgggtcg ggactgcacc cgcgcatcgg ccccggcggc
     1501 tacgagatcg ggtattgggt ccgcaaggac ctcacccgtc gtgggatcgc gacagcagcc
     1561 accaacgccc tgaccgagca cgcgttcacg cggcacgaca tcgaccgggt cgagatccgc
     1621 cacgatcgag ccaacctggc gagcgagtgc atcccgcgcc ggctgggcta cgccctggtt
     1681 ggtgaggagc ccagcgtcat cgctgcaccc gagcaaagcg gcgtccatct catttggcgc
     1741 gttacccgcg acgagtggac ggcgcggcag agaccgtaga ctcgccggtt gtggcacttt
     1801 ctatcggcat cgtcgggctg cccaacgttg gcaagtcgac gctgttcaat gcgctgacga
     1861 aaaacaacgt gctcgccgcg aactatccgt tcgccaccat cgagccgaac gtcggcgtag
     1921 tcggcgttcc cgattctcgg ttgaacgcgc tcgctgcgtt gtacagctcg gtgaagatcg
     1981 tgccggcgac ggtgagtttc gtcgacatcg cgggtctggt gaagggcgca tccgagggtc
     2041 agggtctcgg caacaagttc ctcgcgaaca tccgtgaatc cgacgcgatt tgccaggtca
     2101 cgcgggtatt caccgacccc gac
//
LOCUS       DATCBJ010000340         3169 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_333458, whole genome shotgun sequence.
ACCESSION   DATCBJ010000340 DATCBJ010000000
VERSION     DATCBJ010000340.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 3169)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3169)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3169
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_333458"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(107..1171)
                     /locus_tag="VII50_06760"
     CDS             complement(107..1171)
                     /locus_tag="VII50_06760"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase"
                     /protein_id="HEY5050584.1"
                     /translation="MKALVLSGGAGTRLRPITHTSAKQLVPVANKPVLFYGLEAIRDA
                     GITDVGIIVGDTRAEIEAAVGDGSALGIRATYIPQDAPLGLAHCVLIARDFLGDDDFV
                     MYLGDNFIIGGITDLVDEFKRGGADAQILLTKVDNPQQFGIAELDDAGHILRLVEKPA
                     EPKSDLALVGVYMFGPSIHEAVNSIAPSRRGELEITDAIQWLVDHGKDVRPHMVTGYW
                     KDTGRLEDMLECNRKVLESVEPSCRGNVDDVSRIIGRVVIEEGATIVRSTVRGPAIIG
                     RGTRIVDSYIGPFTSIYHDCLIDSTELEHSIVLEATTISGVSRIEDSLIGKEVEVSPS
                     TAMPRAHRLMLGDHSKVSLA"
     gene            complement(1249..2799)
                     /locus_tag="VII50_06765"
     CDS             complement(1249..2799)
                     /locus_tag="VII50_06765"
                     /inference="COORDINATES: protein motif:HMM:NF025382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4232 domain-containing protein"
                     /protein_id="HEY5050585.1"
                     /translation="MRALRHGLTVAAVGMVVAISGCAAPNQTAQNAGASSVVSSAPTQ
                     SLAAPPMGSPAAPAPASAPATPSVVPVDETSAAPWVDAPGAMFLSSPLPVAALPTTGR
                     ACRASDLRASGDIGAPGGGGHTPETFEFVDTSSTPCVLKGFPRVVATEPGKPAVVAVD
                     GGFFVGQEKSATMSPGSYTTLSLETERDCDARYANANQWPTLIYHTATVAIPGGGSMV
                     VHDVFDVECGLFTGQFGVAQPQPEYTQSVIQGATAHIELPSAVHAGTTLHYVIDLTNP
                     TANDMSLAPCPSYQQGIGEGGTDPLEFNCPAVHLLPAHEAQRFAMELAVPADFPTGPA
                     EVYWNVAATTDPDISACGSVDVVCADTPCAADQLSAAIIGPGAVPGLPNMMGLKGDVT
                     AVPLTVINRSNAKCSVRGAPSVDLRTGDGTDLGLPQADEPAMALVGPRMPTPTVVLAP
                     GEAATTTLYWYSDFCGSDPNPLTVTITLPANGATVESTPTGGWRPPPCPDATPGHGQV
                     GADPFRAG"
     gene            2958..>3169
                     /locus_tag="VII50_06770"
     CDS             2958..>3169
                     /locus_tag="VII50_06770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5050586.1"
                     /translation="MSDLTVLPPELNPRGPGRPIKPVKPGNGRNGWRSPLGIIAAVLA
                     VCVLAASGLLYTRYLHYDHNLKKAAGV"
BASE COUNT          511 a         1045 c         1076 g          537 t
ORIGIN      
        1 gggccgccga tcgccgtccc gatgcttcgg ttggtgggtc gagcggcggc ctggcctgga
       61 tggtccgcgg taccggcggg atcggagata tcaagcatgc tggtgctcag gcgagcgaca
      121 ccttgctgtg gtcgcccagc atcaggcggt gggcacgcgg catggcggtc gagggtgaga
      181 cttcgacctc cttgccgatc agcgaatcct cgatccgact gacgccggag atcgtcgtcg
      241 cctcgagcac gatcgagtgc tcgagctcgg tcgaatcgat gaggcagtcg tggtagatcg
      301 acgtgaacgg gccgatgtag ctgtcgacga tcctggtgcc gcggccgatg atcgccggcc
      361 cgcgcacggt cgaccgcacg atcgtcgcgc cctcctcgat gacgacccgg ccgatgatgc
      421 ggctcacgtc gtcgacgttg ccgcgacagc tcggctcgac cgactcgagc accttgcgat
      481 tgcactcgag catgtcttcg agccggccgg tgtccttcca gtagccggtg accatgtgcg
      541 gccggacgtc cttgccgtga tcgaccagcc actggatcgc gtcggtgatc tcgagttcgc
      601 cgcggcggct cggcgcgatc gagttgaccg cctcgtggat gctcgggccg aacatgtaga
      661 ccccgacgag agcaaggtcg ctcttcggct cggccggctt ctcgacaaga cgaagaatgt
      721 gtccggcatc atcgagttcg gcgatcccga actgctgcgg gttgtcgacc ttcgtgagca
      781 ggatctgtgc gtcggcgccg ccgcgcttga actcgtcgac tagatcggtg atgccaccga
      841 tgatgaagtt gtcgcccagg tacatcacga agtcgtcgtc gccgaggaag tcgcgcgcga
      901 taagaacgca gtgcgccagc ccgagtgggg cgtcctgggg gatgtaggtg gccctgatcc
      961 cgagcgcgga gccgtcgccg acagcagcct cgatctcggc ccgggtgtct ccgacgatga
     1021 tcccgacgtc ggtaatgcca gcgtcacgga tggcctcgag gccgtagaac agcaccggct
     1081 tgttggcgac ggggaccagc tgtttcgccg aggtgtgggt gatcggccgc aaccgcgtgc
     1141 cggcgccacc ggacaaaacg agtgctttca tgcagcgagg ttaccggagc gcccgtcgcg
     1201 cctcctgtga agagtctggg gatcgcgctt tgccaggcct ttccacgctc agcccgcccg
     1261 gaacgggtca gcgccgactt ggccgtgccc aggtgttgcg tcggggcacg gcggtggccg
     1321 ccagccgccg gtcggcgtcg attcgaccgt cgctccattg gccggcaacg tgatcgtgac
     1381 cgtcaacggg ttgggatcag acccgcagaa gtccgagtac cagtaaaggg tcgtggtggc
     1441 agcttcgccc ggcgcgagga cgacggttgg cgtgggcatc cgtggcccca ccagtgccat
     1501 cgccggctcg tcggcttgcg ggaggccgag gtcggttccg tcgcctgtac gcaggtcgac
     1561 ggaaggtgct ccccgaaccg agcatttcgc gttcgaccga ttgatcacgg tgagcgggac
     1621 ggcggtgaca tcgcccttca ggcccatcat gttcggcaac cccgggacag cgcccggccc
     1681 gatgatcgcg gcgctgagct gatcggccgc gcagggggtg tccgcacaga ccacatcgac
     1741 cgacccgcac gcgctgatgt cggggtcagt ggtcgcagcg acattccagt agacctctgc
     1801 cggaccggtg gggaaatcgg ccggtaccgc aagttccatc gcgaagcgtt gtgcctcatg
     1861 ggcaggcagc aggtggaccg cggggcagtt gaactcgagc ggatctgtgc cgccctcacc
     1921 gatcccctgc tgatagctcg ggcagggcgc gagcgacatg tcgttcgcgg tcggattcgt
     1981 caggtcgatg acatagtgca gggtggttcc cgcatggacc gcactcggca gttcgatgtg
     2041 cgcagtcgct ccctggataa cggactgggt gtattcgggc tgcggttgcg cgacaccgaa
     2101 ctgacccgtg aacaagccgc attcgacgtc gaaaacgtcg tgcaccacca tgctgccgcc
     2161 gccaggtatc gccaccgtcg cggtgtggta gatcaacgtc ggccactgat tcgcattcgc
     2221 gtagcgcgca tcgcagtcac gctcggtctc gagactcagc gtggtgtagc tgcccgggct
     2281 catggtcgcg gacttctctt gtccgacgaa gaagccgccg tcgaccgcga cgaccgcggg
     2341 cttgccgggc tccgtcgcga ccacccgcgg gaagcctttc agcacgcacg gcgtcgagct
     2401 cgtgtcgacg aactcgaagg tctcgggggt gtgcccaccg ccgccaggtg cgccaatatc
     2461 gccggatgcc ctgagatcgg aggcccgaca tgctcggccg gtcgtcggca gcgcagcgac
     2521 tggcagtggc gatgagagaa acatcgctcc tggcgcgtcg acccagggag cagcactggt
     2581 ctcgtcgacc ggcacgactg acggcgtggc cggcgccgac gctggcgctg gcgctgcggg
     2641 cgaccccatc ggcggtgctg cgagcgactg cgtcggagct gacgacacca cgctcgacgc
     2701 acccgcgttc tgtgcagtct ggttcggcgc cgcacagcct gagatcgcga cgaccatgcc
     2761 gactgcagcg accgtcaggc catgccgcag cgcacgcatg caacgaagga cgccgccccg
     2821 gcgtccttcg ttgcatgggg cccaatcgag cgaccgcaat cggcgccgcc gtcctgtgtt
     2881 tggccgcgtc gctcccgatg tcggcgccgt acggccgtta gcctcgaaac cacaccaacc
     2941 actggagact caccaccgtg tccgatctga ccgtcctgcc cccggaactc aatccgcgcg
     3001 ggccagggcg tccgatcaaa ccagtcaaac caggcaacgg ccgcaacggc tggcgcagcc
     3061 cgctcgggat catcgcggca gtgctggcgg tctgcgtgct cgccgcgagt ggtctcctct
     3121 acacccgcta cctgcattac gaccacaacc tgaagaaggc cgcgggcgt
//
LOCUS       DATCBJ010000341         3840 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_496965, whole genome shotgun sequence.
ACCESSION   DATCBJ010000341 DATCBJ010000000
VERSION     DATCBJ010000341.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 3840)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3840)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3840
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_496965"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..847
                     /locus_tag="VII50_06775"
     CDS             <1..847
                     /locus_tag="VII50_06775"
                     /inference="COORDINATES: protein motif:HMM:NF021214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome c oxidase assembly protein"
                     /protein_id="HEY5050587.1"
                     /translation="AWLAGIASLLLITLGGLGAHAHTLLWVYTVQVLVLLLGTPVLLA
                     YGRPLALAADALPERVAARLLDARAGVTLRLASNPAVGPLVVPVVLAGIFFSPVLTVT
                     LEHSWAYYLLQLALVGIGLVIAIGLVGDGAERDSSFALAATVAIAFVELLFDAIPGIV
                     LRLRTHLVATAYWTRVHHSGRLSALTDQQHAGAALWFIAEFADLPFLLIVIRRWIRVD
                     QLDAVRIDRELDASATGGAVVPGAAAGAPGGGQGENVPEFTTPWWETDPTRLRGHPIQ
                     REQQD"
     gene            complement(929..1864)
                     /locus_tag="VII50_06780"
     CDS             complement(929..1864)
                     /locus_tag="VII50_06780"
                     /inference="COORDINATES: protein motif:HMM:NF015995.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein"
                     /protein_id="HEY5050588.1"
                     /translation="MKRKVLIIGAAGVAIAMSLSACSSSKKSSSSGATIASKLILGGP
                     PEFQTRVDGIPGLKNNYGVVFGSYKVLDTGGPVTVAALKNGQVDAADLFTTDPSIAAN
                     SFVILSDPKSNFAAQNVLPIINKAKATAGVTKTLNAISAKLDTPGLGALMVKVITNKQ
                     DPDAVAKGWLTTEGLDTTGTDASGVSIKVGSANFPENVLLADIYADALKDQGATISET
                     LNIGSREKYVPALKDGSIDLLPEYNGSILQYLDPKATATTPDAVLAALKTALPANLIV
                     LNQSAAQDSDAIVVTQATASKYNLKSIADLAKSAA"
     gene            complement(1945..2709)
                     /locus_tag="VII50_06785"
     CDS             complement(1945..2709)
                     /locus_tag="VII50_06785"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY5050589.1"
                     /translation="MIGNVFSWLTDPGHWRGGNGLIGIGTQLQAHITYSFVAVAIALA
                     IALPLGLFIGHTGRLAFIVSAANTLRALPTVGVLVLLTVMISPNFHGRTDFGYLIPTE
                     IVLVLIAIPPILSNTYAGVQNVDGAVRDAASAMGMTGSQVLRQVEFPCSLPLIFSGLR
                     SASLQVIATATIASYLPLGGLGRFIYDGLAQQDFPQMIGGGVLVALLALLTDSVLAFI
                     QRHVVSRGVSGRFSKKAMPARGPAAADIVEAEVAAV"
     gene            complement(2706..3425)
                     /locus_tag="VII50_06790"
     CDS             complement(2706..3425)
                     /locus_tag="VII50_06790"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY5050590.1"
                     /translation="MTIAGSLPSSAGVASSSPWHFWAYFNAHLHQDLTWLWDHVWLSA
                     LPVVIGLAIALPLGWLASRYRWIYPPMLSISGLLYTIPSIALFVLIPPLLGLKALNPI
                     QVPIALTIYSVALLVRVVADGLGSVPEDVVQAATAMGFGPIQRLFKVELPIAVPVIAA
                     GLRVATVSNVSIVAVAGTIGLANLGQLFTTGFQLSSPTAYYPPIVLGIVLCVALALTF
                     DLVIVATNRLLTPWRRAVQGR"
     gene            complement(3422..>3840)
                     /locus_tag="VII50_06795"
     CDS             complement(3422..>3840)
                     /locus_tag="VII50_06795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5050591.1"
                     /translation="GYRALGFEPVSGLQLDPETTVRVGSDVSAATAIARDGWVLVVDD
                     HESPQGWLAVGDDATRASHQITPPLLHRGGTVAGVNGTFRSVLDAALSSPTGRGVIVD
                     QAGRFAGTITAASVVRLLEHQHPPAEHQQSAEVTHP"
BASE COUNT          645 a         1191 c         1335 g          669 t
ORIGIN      
        1 ggcctggttg gcgggtatcg cgagcttgtt gctcatcacg ttgggtggcc ttggggcaca
       61 cgcgcacacg ctgttgtggg tctacacggt ccaggtcttg gtgcttctct tggggacgcc
      121 ggtgctgctg gcctatggcc ggccactggc tttggctgcc gacgcgctgc cggaacgggt
      181 ggctgcccgg ctactcgacg cacgggccgg cgtcacactg cgcctcgcgt cgaatccggc
      241 cgtgggcccg ctggttgtcc ctgtcgtgtt ggccggcatc ttcttcagcc cagtgctcac
      301 cgtgacgctc gagcactcgt gggcgtatta cctgctccag ttagccctgg tgggcatcgg
      361 tctggtcatc gcgatcggct tggtcggcga cggcgccgag cgggacagct catttgccct
      421 agcggcgacg gtggcgatcg cgttcgttga gctgcttttc gacgcgatcc ccggcatcgt
      481 cttgcggctg cgtacccacc tcgtcgccac cgcgtactgg acgcgggtgc accactcggg
      541 ccgactcagc gcgttgactg accaacagca tgcgggtgcg gcgctgtggt tcatcgccga
      601 gttcgccgac ttgccgttcc tgctgatcgt gatccggcgc tggatccggg tcgaccagct
      661 cgacgccgtc cgcatcgatc gcgaactcga tgcgagcgcg accgggggtg cggttgtacc
      721 gggcgccgcg gccggcgctc ctggcggcgg gcagggcgag aatgtgccgg agttcacgac
      781 cccgtggtgg gagacggatc cgacccggct gcgtgggcac ccgatccagc gcgagcagca
      841 ggactagcac cggccgctgc ggcccgggac cttgaacgtc ccgggccgca cgctcccgcc
      901 gaagcgagct gccagtaatg accgacgatc aggcagcgga cttcgcgagg tcggcgatcg
      961 acttcaggtt gtacttgctg gcggtcgcct gggtgacgac gatcgcatcg ctgtcctgcg
     1021 ccgcggactg gttgagcacg atcaggttcg ccggcagcgc cgtcttcaat gccgcgagca
     1081 ccgcatcggg ggtggtcgcg gtggccttcg gatcgaggta ctgcaggatc gatccgttgt
     1141 actcgggcaa gaggtcgatc gagccgtcct tgagggcggg cacgtacttc tcgcggctac
     1201 cgatgttgag ggtctccgag attgtcgctc cctgatcctt tagtgcgtcg gcgtagatgt
     1261 cggcgagcag cacgttctcg gggaagttcg ccgagcccac cttgatcgac accccggagg
     1321 cgtccgtgcc cgtcgtgtcg aggccttcgg tcgtgagcca gcccttggcg acggcgtcgg
     1381 gatcttgctt gtttgtgatg accttgacca tcaaggcgcc gagcccaggt gtgtcgagct
     1441 tggcggagat ggcgttcagg gtcttcgtca cgccggcggt tgcctttgcc ttgttgatga
     1501 tcgggagcac gttttgtgcg gcgaagttgc tcttcgggtc gctcaggatc acgaagctgt
     1561 tcgccgcgat gctcgggtcg gtggtgaaca ggtcagccgc gtcgacctgg ccgttcttca
     1621 gtgcggcgac ggtcaccggg ccaccggtgt cgagcacctt gtagctgccg aacacgacac
     1681 cgtaattgtt cttcagaccg gggatgccgt cgacgcgagt ctggaactcc ggcgggccgc
     1741 cgaggatcag cttgctggcg atggtggcgc cgctggacga ggacttcttc gaactgctgc
     1801 acgcggacag actcatggcg atcgccacac ctgcagcacc gatgatcaat accttgcgct
     1861 tcatgagact ccttatgtct ggcggatcta gccaggtccg ttcgagaggg tcgagcatcg
     1921 agggtttcgt tgacgttgag ttcgttagac ggccgcgacc tccgcttcga cgatgtcggc
     1981 ggcggctggc ccgcgcgccg gcatggcttt cttggagaag cggcctgaca caccgcggga
     2041 aacaacgtgt cgctgaatga acgcgagcac cgaatcggtg agaagtgcca gcagcgcaac
     2101 gagaacgccg ccaccgatca tctgcggaaa gtcttgttgg gccagcccgt cgtagatgaa
     2161 cctgccgagg ccaccgaggg gcaggtacga cgcgatcgtg gcggtggcga tgacctgcaa
     2221 tgaggcgctg cgaaggccgg agaagatgag cggcaagctg cacgggaact cgacctggcg
     2281 cagcacctga gaaccggtca tgcccatcgc ggacgccgcg tcgcgaaccg cgccgtcgac
     2341 gttttgcaca ccggcatagg tgttggacag gatcggcgga atcgcgatca acacgagcac
     2401 gatctcggtc gggatcaggt agccgaagtc ggtgcgaccg tggaagttcg gcgagatcat
     2461 cacggtcagc aacacaagga caccgacggt tggcagtgcg cgcaaggtgt tcgccgcact
     2521 cacgatgaac gccagcctgc ccgtgtgccc gatgaagagc ccgagcggta gagcgatcgc
     2581 cagcgcgatc gctacggcga cgaacgagta ggtgatgtgg gcttgcagct gagtgccgat
     2641 gccgatcaag ccgttgccgc cacgccaatg tccagggtcg gtcagccagc tgaaaacgtt
     2701 gccgatcatc ggccctgcac cgcccgccgc cacggtgtca ggagccggtt cgtcgccacg
     2761 atgacgaggt cgaaggtcag tgccagcgcg acgcacagca cgatgccaag cacgatcggc
     2821 gggtagtacg cggtcgggct agacaattgg aaaccggtcg tgaacagctg gccgagattg
     2881 gccaggccga tcgtgcctgc gaccgcgacg atgctgacat tcgacacggt cgccacccga
     2941 agcccggcag cgataaccgg gacggcgatg ggcagctcga ccttgaacag tcgctggatc
     3001 ggcccgaacc ccatggcggt tgccgcctgc accacgtctt ctggcaccga accgagaccg
     3061 tcggcgacca cgcgcaccag cagcgcgacg gagtagatcg tcaacgcgat cggaacctgg
     3121 atcgggttca gggctttgag gccgagcagc ggcgggatca gcacgaacag cgcgatcgac
     3181 gggatcgtat agagcaggcc ggaaatggaa agcatcggtg gatagatcca ccggtatcgg
     3241 ctcgccagcc atccgagcgg caacgcgata gcgaggccga tcacgactgg tagcgccgag
     3301 agccacacgt ggtcccacag ccaggtcagg tcttggtgca gatgcgcgtt gaagtacgcc
     3361 cagaaatgcc agggggacga cgaggcgacg ccggccgacg aaggcagcga gccagcgatg
     3421 gtcatgggtg cgtcacttcc gcactctgtt gatgctcggc cggcgggtgc tggtgttcga
     3481 ggagccggac cacgctcgcg gccgtgatcg tgccggcgaa ccgacccgcc tggtcgacga
     3541 tgaccccgcg gccggtcggt gacgacaacg cagcgtcgag cacggaccgg aaggtgccgt
     3601 tcacgcccgc gacggtgccg ccgcggtgca gtaacggtgg cgtgatctgg tgcgaggcgc
     3661 gggtggcgtc atcgccaacc gcaagccagc cctgtggcga ctcgtggtcg tcgacgacga
     3721 gcacccagcc gtctcgcgcg atggcagtcg cggcactgac gtctgagccg acccgcacgg
     3781 tggtttcggg atcgagttgc agccccgaga cgggttcaaa gccgagcgcc cggtacccgc
//
LOCUS       DATCBJ010000342         2873 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_113895, whole genome shotgun sequence.
ACCESSION   DATCBJ010000342 DATCBJ010000000
VERSION     DATCBJ010000342.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2873)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2873)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2873
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_113895"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..669)
                     /gene="tpiA"
                     /locus_tag="VII50_06800"
     CDS             complement(<1..669)
                     /gene="tpiA"
                     /locus_tag="VII50_06800"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009647772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HEY5050592.1"
                     /translation="MAPKATRKPLMAGNWKMNLNHLEAIGLVQKLAFSLSDPDYDAVD
                     VAVLPPFTDLRSVQTLVDGDRLRLLYGAQDVSAYSSGAYTGEISAAMLAKLGCAFVVV
                     GHSERRQYHDEDDALVNAKVKATIAAGMTPILCIGEQLDVRRAGEQVQHTLAQLDGGL
                     ADVTAEHVAEIVIAYEPVWAIGTGEVATPQDAQEVCGAIRGRLVELYETSVAAAARVL
                     YGGSG"
     gene            complement(672..1895)
                     /gene="pgk"
                     /locus_tag="VII50_06805"
     CDS             complement(672..1895)
                     /gene="pgk"
                     /locus_tag="VII50_06805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016461700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HEY5050593.1"
                     /translation="MRTVDDLEVAGRTVLVRADLNVPLDSSGDGPPVITDDKRIRASV
                     PTIRELASRGARVVVMAHLGRPKGGVNPALSLRPAASRLSELLGRPVGFPDDANDGIA
                     GISAQAVVEETPLGGVVMLENLRFEAAETSKDDAERAVFAGQLAALAGAGGSYVGDGF
                     GAVHRKHASVYDLPQLLPHAAGGLVVAEVSVLRRLTDELERPYVVVLGGAKVSDKLGV
                     IANLLDKVDRLLIGGGMAYTFLAAQGYEVGNSLLEKDQVDVVRGLIADADKRGVDLVL
                     PVDIVAATHFAADAPHIVVPSSQIPADREGLDIGPQTCALFASKLADARTVFWNGPMG
                     GFEFDAFAGGTKALASALTAVDGLTVVGGGDSAAAVRKLGFEESDFGHVSTGGGASLE
                     YLEGKTLPGLQVLED"
     gene            complement(1906..>2873)
                     /gene="gap"
                     /locus_tag="VII50_06810"
     CDS             complement(1906..>2873)
                     /gene="gap"
                     /locus_tag="VII50_06810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HEY5050594.1"
                     /translation="DGDRAVAASHGSNIEIVAVNDLTDTKTLAHLLKYDTVLGTFAGE
                     VSFGEGSITVDGKEIKVLSQRDPALLPWGELGIDIVIESTGHFTDADKAKAHITAGAK
                     KVIISAPAKGEDGTFVLGVNDDDYDNDKHHIISNASCTTNCVAPLAKVLQDSFGIVKG
                     FMTTVHAYTNDQVILDFPHKDLRRARAAAQNIIPTTTGAAKAVALVLPELKGKLDGFA
                     LRVPVPDGSITDLVVELEREVTRDEVNAAYKAAADGPLKGYLVYTEDEIVSSDIVGTP
                     ASCTFDSKLTMVSGKTVKVLGWYDNEWGYSSRLADLTALVAARLS"
BASE COUNT          470 a          987 c          931 g          485 t
ORIGIN      
        1 gcccgagccc ccgtacagga ctcgcgcagc ggcggcaacc gacgtctcgt acaactccac
       61 caggcggccg cggatggcgc cgcagacttc ctgggcgtcc tggggcgtgg ccacctcgcc
      121 ggtgccgatc gcccagaccg gctcgtaggc gatcacgatt tccgccacgt gctcggccgt
      181 gacgtcagcc agtccgccgt cgagctgagc caaggtgtgc tgcacctgct cccctgctct
      241 gcgcacgtca agctgctctc cgatacacag gatcggcgtc atgccggccg cgatggtggc
      301 cttgaccttc gcgttgacca gcgcgtcgtc ttcgtcgtga tactggcgtc gctcggaatg
      361 accgacgacg acgaacgcgc agccgagctt cgcgagcatc gcggccgaga tctcacccgt
      421 gtaggccccc gagctgtacg ccgagacgtc ctgcgcgccg tacagcagcc gcaaccgatc
      481 gccatccacc aatgtctgca cgctgcgaag gtcggtgaac ggaggcaaga ccgcgacgtc
      541 gacggcgtcg tagtcggggt cagacaacga gaacgccaac ttctgcacga ggccgatggc
      601 ttcgaggtgg ttcaggttca tcttccagtt gcccgccatc aagggcttgc gggtcgcttt
      661 gggcgccatc gtcagtcctc caagacctga agcccgggaa gcgtcttgcc ctcgaggtac
      721 tcaagactgg cgccgccacc cgtcgagacg tggccgaagt cgctctcctc gaacccgagc
      781 ttgcgcaccg cagctgccga atcgcccccg ccgaccactg tcaagccgtc gacggctgtc
      841 agagccgaag ccagcgcctt cgtcccgccc gcgaaggcat cgaactcgaa cccccccatc
      901 ggaccgttcc agaagaccgt ccgggcgtcg gcgagtttcg aggcgaacag cgcgcacgtt
      961 tggggaccga tatcgaggcc ctcccgatcg gcaggaatct gcgacgacgg gacgacgatg
     1021 tgtggcgcat cggccgcgaa gtgggtcgcc gcgacgatgt cgacgggcag gaccaggtcg
     1081 accccgcgct tgtccgcgtc ggcgatcaac ccgcgcacca cgtcgacctg atccttctcc
     1141 agcagcgagt tgcccacctc gtacccctgg gccgccagga aggtgtacgc catgccgccg
     1201 ccgatcaaca gccggtcgac cttgtcgagc aggttggcga tgacaccaag cttgtcggag
     1261 accttggcgc cgccgaggac gacaacgtag ggccgctcga gttcgtcggt cagccgccgt
     1321 agcaccgaaa cttcggcaac cactaggccg ccggccgcat gcggcagcag ctgcggcagg
     1381 tcgtaaacgc tggcgtgctt gcggtggacc gcgccaaacc cgtcgccgac gtagctgccg
     1441 ccggccccgg ccagcgcggc aagttggccg gcgaaaaccg cccgctcagc gtcgtccttg
     1501 ctcgtctccg ctgcttcgaa gcgcaggttc tcaagcatca cgaccccgcc gagcggggtc
     1561 tcctcgacga ccgcctgcgc cgagattccg gcgatcccgt cattcgcatc gtcgggaaag
     1621 ccgacgggcc ggccgagcaa ctccgatagt cgcgaggccg ccggccgcaa cgacaacgcc
     1681 ggattcacgc cgcccttggg ccggccgagg tgcgccatca cgacgacccg cgcgccccta
     1741 ctcgccagtt cgcggatagt cgggacactg gcccggatgc gcttgtcgtc ggtgatgacc
     1801 ggcgggccgt caccgctgga gtccagcggc acattcaggt cggcccgaac aagcacggtc
     1861 cggccggcga cctcgaggtc gtcgacggtg cgcatcgccc gccgcctagc tgagtcgtgc
     1921 ggcaacgagc gccgtgaggt cggccagccg gctcgagtag ccccactcat tgtcgtacca
     1981 gccgaggacc ttgacggtct tgccgctgac catggtgagc ttggagtcga aggtgcacga
     2041 ggcaggggtg ccgacgatgt ctgacgagac gatctcgtcc tcggtgtaga ccaggtagcc
     2101 cttcagcggc ccgtcggcgg cggccttgta ggccgcgttc acctcgtcgc gcgtgacttc
     2161 gcgctcgagc tccacgacaa ggtcggtgat cgaaccgtcg ggaaccggca cccgcagcgc
     2221 gaacccgtcg agcttgccct tcaactccgg cagcaccaac gccacggcct tcgccgcacc
     2281 ggtcgtcgtc gggatgatgt tctgcgccgc ggcgcgtgcc ctgcgaaggt ccttatgcgg
     2341 gaagtcgagg atgacctggt cgttggtgta ggcgtgcacc gtcgtcatga agcccttgac
     2401 gatgccgaaa ctgtcctgca ggaccttggc gagcggggcg acgcagttcg tcgtgcacga
     2461 ggcgttgctg atgatgtggt gcttgtcgtt gtcgtaatcg tcgtcgttca cgccgaggac
     2521 gaacgtgccg tcctcacctt tggcgggtgc cgagatgatg acctttttcg cgccggcggt
     2581 gatgtgcgcc ttcgccttgt cggcgtcggt gaagtggccg gtcgactcga tgacaatgtc
     2641 gatgccgagc tcgccccatg ggagcaacgc agggtcgcgc tgtgacagca ccttgatttc
     2701 tttgccgtcg acagtgatcg aaccctcgcc gaagctgacc tcgcccgcga acgtgccgag
     2761 aaccgtgtcg tatttcagca ggtgagcgag cgtcttggtg tccgtgaggt cgttgaccgc
     2821 gacgatctcg atattgctgc cgtgggaagc cgcgacggct cgatcaccgt cga
//
LOCUS       DATCBJ010000343         3633 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_85861, whole genome shotgun sequence.
ACCESSION   DATCBJ010000343 DATCBJ010000000
VERSION     DATCBJ010000343.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 3633)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3633)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3633
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_85861"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..393
                     /locus_tag="VII50_06815"
     CDS             <1..393
                     /locus_tag="VII50_06815"
                     /inference="COORDINATES: protein motif:HMM:NF013331.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II truncated hemoglobin"
                     /protein_id="HEY5050595.1"
                     /translation="YDRVEVDELLSPLFPGGVTREHRLHVIAWWCEVFGGPTRYTDEL
                     GGYERMLAKHHDLAITPEQRFRFASLMSLAADDAKLPDDPEFRSAFVAYVEWGTRLAQ
                     HNSQPGAEVVEHAPVPRWGWGEAPPYQP"
     gene            529..1293
                     /locus_tag="VII50_06820"
     CDS             529..1293
                     /locus_tag="VII50_06820"
                     /inference="COORDINATES: protein motif:HMM:NF015921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5050596.1"
                     /translation="MHGALRVPEITIYFWVIKALSTAMGESTSGYLVHSIDPVVAVAI
                     GFVGFCGALALQLSMRRYLAWTYWLAVVGVGVFGTMAADVLHVRFGVPYAASTVLYAA
                     VLLAVFIAWQRTEGTLSIHTVNTPRRELFYWAVVVATFAMGTALGDLTATTFHLGYFG
                     SGLLFAGVIAIPAVGYWRLGWNAIFSFWFAYVATRPLGASFADWMGKPQSVGGLGWGE
                     GPVALVLTVAIFCLVAYLAITRRDLQRADRSPATRP"
     gene            1418..1885
                     /locus_tag="VII50_06825"
     CDS             1418..1885
                     /locus_tag="VII50_06825"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEY5050597.1"
                     /translation="MRRLNIADPTFTYDPGDPDGFRAGMLRFGPQVGAKRTGTSVYEL
                     PPGQAICPYHYEYGEEEWLLVLSGRPTLRTPDGSERLEPWDAVCFPEGPTGAHALRNE
                     TDEVVRVLMYSTVSPTAVSVYPDSGKIGVWVDNPDDTLLFPRKSATDYYSGEV"
     gene            complement(1891..2592)
                     /locus_tag="VII50_06830"
     CDS             complement(1891..2592)
                     /locus_tag="VII50_06830"
                     /inference="COORDINATES: protein motif:HMM:NF024986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HEY5050598.1"
                     /translation="MGGASIPLTCLASEVRRAVPSPQEPRLPLRVDQGQVAELHHDLA
                     VNETELAGVSLGDQRAHGVMFAAVKLSSVDLSGSNLEHLRITDGALRQCNLANVQAHR
                     ASLTRVTIEGSRMTGLAVPEAALRDLTLRDCRIDLASFGFSRLERVTFEDCLLQQTDF
                     LEAQLDAVRFYRCDLTRADFRGARLKRCEFRRSNLTGLEGVESLRGAAMEWPDIVEMA
                     GVWAAALGIEILEAD"
     gene            2942..>3633
                     /locus_tag="VII50_06835"
     CDS             2942..>3633
                     /locus_tag="VII50_06835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5050599.1"
                     /translation="MRLVATTRGLGCLEVGRVQDGNVGVVGQDHAFSDDGKFHPLPVA
                     LSATSPACAPLDRRGRLFLNVNVGDVPASGVNASGVDVSGSCLPPNAHQPVGPFCPGR
                     DERNLAYGLLGPRATALTYTGENGAQRTIKLHSPDGAYLIVTRAVEAPGSINNAISAS
                     LNPNAPITTVTYSNGLRCNFAEPLPTVHHECRLPLGYVPRPTPHYTQAQLASPVRAIA
                     RPAGQGRWKLTVS"
BASE COUNT          576 a         1221 c         1209 g          627 t
ORIGIN      
        1 tacgaccggg tcgaggtcga cgagttgctg tcgcccttgt tcccaggcgg agtgacgcgg
       61 gaacatcgac tgcacgtgat cgcttggtgg tgtgaagtgt tcggtggtcc gacgcgctac
      121 accgatgagc tcggcggcta cgagcgcatg ctggccaagc atcacgatct cgcgatcact
      181 ccggagcagc ggttccggtt cgcgtcgctg atgagtcttg ctgccgacga cgcaaagctg
      241 cccgacgacc ctgagttccg ctcggcgttc gtcgcgtacg tcgagtgggg taccaggctg
      301 gcgcagcaca actcgcaacc gggcgccgag gtcgtcgagc acgcgcccgt cccacgctgg
      361 ggctggggcg aggcgccccc ctaccagccg tagccacgac cacgagcctg tcgctacgcg
      421 ctgatgcggt tagatcacgc gggtcgagta ccggtaggcg gcgcgtggtc ggctgtgccg
      481 tggaagactg cggcggtgaa cgaccgctct cgcgttaggc ggctgacggt gcacggcgcc
      541 ctccgcgtcc cggagatcac catctatttc tgggtgatca aggcgctgtc gaccgcgatg
      601 ggcgagtcaa cgtcgggcta tctcgtccac tcgatcgacc cggtcgtcgc cgtcgcgatc
      661 ggcttcgtcg ggttttgcgg cgccctggcc ctgcagctgt cgatgcgtcg ctatctggca
      721 tggacgtact ggttggcggt ggtcggcgtc ggtgtgttcg gcacgatggc cgcggacgtg
      781 ctccacgtgc gtttcggcgt cccgtacgcg gcgtccaccg tgctctacgc agcagtgctg
      841 ctcgcggtgt tcatcgcctg gcagcgcaca gagggcacgc tttcgatcca caccgtcaac
      901 acgcctcgtc gagaactgtt ctactgggcg gtcgtggtcg cgacgttcgc gatgggaaca
      961 gcgctcggcg acctcacggc gacaaccttc cacttgggat acttcggctc cggtttgctg
     1021 ttcgccggcg tcatcgcgat cccggcagtc ggctactggc gcctcggctg gaacgcgatc
     1081 ttctcgttct ggttcgccta cgtcgcgacc cggccgctcg gcgcctcgtt cgccgactgg
     1141 atgggcaaac cccaaagcgt gggaggcctc ggctggggcg aagggccggt cgccctcgtg
     1201 ctgaccgtcg ccatcttctg cctggtcgcc tacctcgcga tcacccgcag agacctccag
     1261 cgcgccgacc gatcgcccgc tacccgacca tagtcgcgac gagggggctg tcccgctgca
     1321 cgacgagccg cagcacggtg ggctcgaaat acgcgcgagc gacacccggt tgcttgcgcg
     1381 gatcgtttcc gtcggacgtg gctgtgacga ttgccgtatg cgccgactca acatcgccga
     1441 tccgaccttc acctatgacc ccggtgatcc ggacggtttt cgggcgggga tgctgcgctt
     1501 tggcccgcag gtcggggcga agcggaccgg cacgagtgtg tacgagcttc cgccgggcca
     1561 ggcgatctgt ccctaccact acgaatacgg cgaggaggaa tggctgctcg tcctgtccgg
     1621 tcgcccaacg ctccgaacgc ccgacggcag cgagcgactt gagccgtggg atgcggtgtg
     1681 cttccccgag ggcccgacag gcgctcacgc cctccgcaac gagaccgacg aagtcgtgcg
     1741 ggttctcatg tactccaccg tgagtccgac agcggtgtcg gtctacccgg actccggcaa
     1801 gatcggcgtg tgggtggaca atccggacga cacgctcctc ttcccccgga agagcgcaac
     1861 cgactattac agcggagaag tctgaccggc tcagtccgct tcgaggatct caatgcccag
     1921 ggcggcggcc cagacgccgg ccatctcgac gatatctggc cactccatcg cggcgccgcg
     1981 gaggctctcg acgccctcaa gcccagtgag gttgctgcgc cggaactcgc aacgcttgag
     2041 gcgagcgcca cggaagtcgg cccgcgtcag gtcgcagcgg tagaagcgga cggcatccag
     2101 ctgcgcctcc aagaagtcag tctgctggag cagacagtcc tcgaaggtca cgcgttcgag
     2161 gcgcgagaag ccaaacgacg cgaggtcgat gcgacagtca cggagcgtga ggtcacgcag
     2221 cgccgcctcc ggcacggcga gtccggtcat ccggctgccc tcgatcgtca cgcgggtaag
     2281 gctcgccctg tgtgcttgca cgttggcgag gttgcactgt cggagcgcac cgtcggtgat
     2341 tctcaggtgc tccaggttgg atcccgagag gtcgacgctc gagagcttga cggccgcgaa
     2401 catcacccca tgcgcacgct ggtctcccaa cgaaacgccc gccagctcgg tctcattcac
     2461 agcgaggtcg tgatgtagtt cggcgacctg gccctgatcg acccgcagcg gcagccgcgg
     2521 ttcctgcggg cttggaactg cgcggcgcac ctccgaagcc aagcatgtca gcgggatcga
     2581 cgctcctccc acgggcgaat tgtcgggtgg gggactgcgt tgtcccgggc gtgggttggg
     2641 ggtagtgtgc cggcgcagcg agcgatgacc gtttctgagg gccatgcgtt tccacataga
     2701 cacgccgacg agcgtcgtcg cctccagctg gcgttggcgg ccgcttgtcg caatcctggt
     2761 tgtcgtggtc ggcggtgcga ctgtcgcgct tgccgcaacc ggtgtgctga acgctgggaa
     2821 gcctgtccgg gacgcaagcg cgctgcccgc cccctcagcg ggaaacggat ctgttcgggc
     2881 aaagagctcg agaatgctga gcgttcgcgc tcatgacccg ggcggaggct tggcgtgggg
     2941 catgcggctt gtcgcgacca cgcggggcct cggctgcctg gaggtcggac gcgtgcagga
     3001 cggcaacgtg ggcgtcgttg gtcaggacca cgcgttcagc gatgacggca agttccatcc
     3061 gcttccggtc gcgctgagcg ccacatcccc cgcgtgtgca ccgctcgatc gccgcggccg
     3121 tttgttcctg aacgtcaacg tcggtgacgt cccggcatcg ggcgtgaacg cgagcggtgt
     3181 cgacgtgtcc ggcagctgct tgccgccgaa cgcccaccag cccgtcggac cattctgtcc
     3241 cggacgcgac gagcgcaacc tcgcctacgg gctactcgga ccgcgggcga cagccctcac
     3301 ctacacgggc gagaacgggg cacaacgcac gatcaagctt cacagtcccg atggcgcata
     3361 cctgattgtg acgcgcgccg tggaagcccc cggatcgatc aacaacgcga tcagcgcatc
     3421 gcttaacccg aacgcgccga tcacgacagt cacctacagc aacggcttgc gctgcaactt
     3481 cgcggaaccg ctccccacag ttcaccacga gtgccgactc ccgctcgggt acgtgcccag
     3541 accaacgccg cactacacgc aggcgcaact tgcgagcccc gtccgcgcca tcgcacggcc
     3601 agcgggacaa ggacgctgga agctcacggt gtc
//
LOCUS       DATCBJ010000344         2952 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_227901, whole genome shotgun sequence.
ACCESSION   DATCBJ010000344 DATCBJ010000000
VERSION     DATCBJ010000344.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2952)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2952)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2952
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_227901"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..723
                     /locus_tag="VII50_06840"
     CDS             <1..723
                     /locus_tag="VII50_06840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5050600.1"
                     /translation="ASVGLNGFSHRRFLNDFYSGSFAGSFARGALFQENEATGDARIS
                     GSAASLCGIESALATADEHALTLALRRLESMYAVVFSFGGIPLIYMGDELAMLNDPQW
                     DDDLAHADDNRWMHRPAMNWAAADRRGDPKTLEGKAFSAIRRLVDARRSLLALRAGGD
                     TEIVPTEINGVLAYRRVHPRSAPFLALTNFSDLDVDVDDDIVRRAGLHAPRHLHSTTG
                     ELIVDAGRIQLPPWGFGWLTGT"
     gene            complement(757..1350)
                     /locus_tag="VII50_06845"
     CDS             complement(757..1350)
                     /locus_tag="VII50_06845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5050601.1"
                     /translation="MFVQIIQGTVTDADALQRSVARWRTEIKPGAHGYLGSTGGVTPD
                     GRGITMVRFESEADAQANSDRAEQGAWWNEASKAFGDDVTFHNCTEVESILGGGNDAA
                     GFVQVLQGRTNDQAQMRSTGLAIQDELHALRPDILGVLVAFHGDGGFSQFLYFSSEEA
                     ARVGEKAIQDDSELSQRLRATIEGDMTFFDLKEPDFD"
     gene            complement(1363..1566)
                     /locus_tag="VII50_06850"
     CDS             complement(1363..1566)
                     /locus_tag="VII50_06850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5050602.1"
                     /translation="MLVAQLSMTDKHWLTLRANLALACIAVVTVGAVASMLKHGPRSN
                     LAMTPSASRSQDVASAHPVRLTL"
     gene            1879..2151
                     /locus_tag="VII50_06855"
     CDS             1879..2151
                     /locus_tag="VII50_06855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5050603.1"
                     /translation="MTATLVVRHKVNDYTAWRGVYDELEPLRLEHGCTGDRVLQLPED
                     SNDVLVTHDFPSVEQAASFAHDPQLKAGMDRAGIAGPPQIEIFAVA"
     gene            2207..2752
                     /locus_tag="VII50_06860"
     CDS             2207..2752
                     /locus_tag="VII50_06860"
                     /inference="COORDINATES: protein motif:HMM:NF024212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEY5050604.1"
                     /translation="MVKVDPDYKAEYPAGDASSTEAFASLVRAGSASLLELDRRIVAT
                     FDVPHPAATMLAVVDGAGEPLTPSQISERVLVASASTTATLDLLERRGWVRRGPNPDD
                     RRSTLVEVTPAGRGITDRLLPGIRAIERSAMDTLTKSERVQLLALLGKVLGRLAELAD
                     EDPIPLDGERNRPARLNRATS"
BASE COUNT          517 a          954 c          964 g          517 t
ORIGIN      
        1 gcatccgtcg ggctcaatgg cttcagccat cgccggtttc tcaacgactt ctacagcggc
       61 agcttcgcag gctcgttcgc gcgcggtgcc ttgttccagg agaacgaggc caccggcgat
      121 gcccggatca gcggcagcgc ggcgtcattg tgcggaatag agagcgctct cgcgacggct
      181 gacgagcatg cgctgacgct cgccctgcgc cggctcgagt cgatgtacgc cgtcgtgttc
      241 tcgttcggtg gtatcccgct gatctacatg ggcgacgagc tggcgatgtt gaacgacccg
      301 cagtgggacg acgatctggc gcatgccgac gacaaccggt ggatgcaccg gccggcgatg
      361 aactgggctg ccgctgatcg gcgcggcgac ccgaaaacgc tggaaggtaa ggctttttcc
      421 gcgatccgca ggttggtcga cgcacgacga tcgttgctcg cgctgcgcgc gggtggcgac
      481 actgagatcg tgccgaccga gatcaacggc gtgcttgcct atcgccgggt gcatccgcgc
      541 agcgcgccgt tcctcgcact gaccaacttc agtgacctcg atgtcgatgt cgacgacgac
      601 atcgtcagac gtgccggtct gcacgcaccg cgccacctgc actccacgac cggcgagctg
      661 atcgtcgacg ccggccgcat tcagcttccg ccgtggggat ttgggtggct caccggcacc
      721 tgaacgcgtc aggtgccggt gcgtcgccta ggccggtcag tcgaagtcgg gctctttcag
      781 gtcgaagaac gtcatgtcgc cctcgatcgt ggcacgcaac cgctgactca gctcggagtc
      841 gtcttggatc gctttctcac ccacacgagc cgcttcctca gagctgaagt agaggaactg
      901 gctgaacccg ccgtcgccgt ggaacgcgac aaggacgccg aggatgtcgg gccgcaatgc
      961 atgcagctcg tcttgaatcg cgaggccagt gctgcgcatc tgggcctggt cgttggtgcg
     1021 tccctgaagc acctgcacga agcccgcggc atcgttgccg ccaccgagaa tcgattccac
     1081 ttcagtgcag ttgtggaagg tcacgtcgtc gccaaaggcc ttcgacgctt cgttccacca
     1141 ggcaccttgc tcggcccgat cgctgttggc ttgcgcgtca gcctcagact cgaagcggac
     1201 catcgtgatg ccccggccgt cgggggtgac cccgccggtg gagcccaggt acccgtgcgc
     1261 gccaggcttg atctccgtgc gccaccgagc cactgatcgc tgcagagcgt cagcatcggt
     1321 caccgtgccc tggatgatct gaacgaacat gtgcgccgcc tttcagaggg tgagcctcac
     1381 agggtgagcc gaggctacgt cctggctgcg cgacgcacta ggggtcatgg cgaggttgct
     1441 gcgcggtccg tgcttcagca tgctcgcgac cgcaccgacc gtgaccacgg cgatgcaggc
     1501 gagcgcgagg ttcgccctga gcgtcagcca gtgcttgtcg gtcatcgaga gttgggcgac
     1561 gagcatgacc agtacggtcc ggccgatgat cgcgatgccg cagtggccta caccggaacg
     1621 tgaactccca gggcgacggc gtccgcttga actaccgctt tcgcctcgcg agcttgagga
     1681 agaagcggac cagcgccagt gcgaaccaca accccatgcc gcgacgctac tcgccccggg
     1741 gtccgcagac ccgcgctcgc gtgggtggtc ttgtagtaat atatctccga gctaaactac
     1801 ttgtctccga ggcatcacat atgtagggtc ctggactagt gaggcagccc gcctccctcc
     1861 acgttgggag ctagtgcaat gaccgcaact ctcgtcgtcc ggcacaaggt caacgactac
     1921 acagcatggc gcggcgtgta cgacgaactc gaaccgctcc gcctcgagca cggctgcacc
     1981 ggcgaccgcg ttctgcaact tcccgaggat tcaaacgacg tgctcgtcac gcatgacttc
     2041 ccgtcggtcg agcaagcggc gagcttcgcg cacgacccgc agttgaaggc ggggatggac
     2101 cgcgcgggaa ttgccggccc gccgcagatc gagatcttcg cggttgcctg agccctatcg
     2161 ccgtcggccg gggaagagcg ctcgctcatc cccggccaag ctcggcgtgg tcaaagtcga
     2221 ccccgattac aaggccgagt atccggccgg cgatgccagc agcacggagg cgttcgcgtc
     2281 gctggttcga gcgggttccg cgagtctgct ggagctcgac cgccggatcg ttgccacctt
     2341 tgacgtaccg caccctgcgg cgaccatgct cgccgttgtc gacggtgccg gcgaaccgct
     2401 gacgccgtcg caaatcagtg agcgcgtcct tgtcgcatcg gcgagtacga cagccaccct
     2461 tgacctgctc gaacgacggg gatgggtgcg gcgcggaccc aaccccgatg accgccgcag
     2521 cacacttgtc gaggtcacgc ccgccggtag gggcatcacc gaccggctgc tcccgggtat
     2581 tcgtgcaatc gagcgcagcg ccatggacac gctgacgaag agcgagcgcg tgcaattgct
     2641 cgcgttgctc ggcaaagtcc tcgggcgatt agccgaactc gccgacgaag accccattcc
     2701 gctggacggc gagcggaacc ggccagcgag actcaaccgt gcaacgagct aaggacgacg
     2761 ctccactgca ggagcgagtt tgggacgatt ctcctgacag agtaggacat tagtccctgt
     2821 ggtgtgaggt caggggagcg gatcatgtgc atgctcggtt tgcccctgct gtgagggcgg
     2881 gggtgaagtg aacgaagggc gatcgagtcg cgccaggacc gcgtgtgagg tgcggagcaa
     2941 ggctggcgtg aa
//
LOCUS       DATCBJ010000345         3090 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_59190, whole genome shotgun sequence.
ACCESSION   DATCBJ010000345 DATCBJ010000000
VERSION     DATCBJ010000345.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 3090)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3090)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3090
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_59190"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            322..1092
                     /gene="lexA"
                     /locus_tag="VII50_06865"
     CDS             322..1092
                     /gene="lexA"
                     /locus_tag="VII50_06865"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HEY5050605.1"
                     /translation="MSPRDAEGPGPLAGVSDFPDGPPDETGLTPRQHRVLDVIRDSVE
                     RRGYPPSMREIGEQVGLTSTSSVAHQLTTLQKKGYLRRDPNRPRAVEVRIPGEIAGTS
                     TSEPRGTREHSGTREHAAPFDETESGNSRPAPAYIPVVGRIAAGGPILAEQAVDDVFP
                     LPRNVVGEGTLFMLKVVGDSMVDAAICDGDWVVVRQQPVAESGDIVAAMIDGEATVKT
                     FKRRDGHVWLMPHNPNYAPIPGDDATILGRVSAVLRKV"
     gene            1130..1933
                     /locus_tag="VII50_06870"
     CDS             1130..1933
                     /locus_tag="VII50_06870"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HEY5050606.1"
                     /translation="MDDEQVLASDGVTYVVQLGDTVRRPVRPFTATIQAYLEHLHDRG
                     FHDAPIPLGYDAQGREVLSFVTGDVPQEPLPDYACTDDVLVALGTLIRRLHDAAADFI
                     PPVDAIWGGIPGTPAGVVPLFDNPELVSHQDYCPGNVVFRTGLPAAFIDFDLSRPTTR
                     VADLVNAMYWWTPLMHPSDRPPAMRDVDVPARARILADAYGMTAQQRALVVPTAVQRA
                     ANAMPAMHAAAQADPVFAKWWDGGLEQRLLRAQEWLATEAPRIDTALRD"
     gene            1978..2769
                     /locus_tag="VII50_06875"
     CDS             1978..2769
                     /locus_tag="VII50_06875"
                     /inference="COORDINATES: protein motif:HMM:TIGR03083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maleylpyruvate isomerase family
                     mycothiol-dependent enzyme"
                     /protein_id="HEY5050607.1"
                     /translation="MSEQHTKPSADALLAAVRNSRARLVETVTSLTPEQLREQSYDTE
                     WSIAQVLSHIATTAVFFKLILQAGLDGQPAPAMDLMRPVWDEWNAKSAQEQAHDGLAA
                     DGEYLAALESLTAQQRADWQLDLFGTHRDFAGLLTMRVGEHAVHTWDVVVALDPSAML
                     APDAVDILIDTLGQLVGWVGKPLDQQLVIGVETDNPSRNFVLTVDAEGATLEPLDARS
                     DADRLLALPAEAFVRLVYGRLDPEHTPSVADDPILEVLRRVFVGF"
     gene            complement(2766..>3090)
                     /locus_tag="VII50_06880"
     CDS             complement(2766..>3090)
                     /locus_tag="VII50_06880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5050608.1"
                     /translation="ADRRFAAIDFDARVELPRRNYLLFGGRLDCALEIGDWPTPEWFL
                     PHSPNIFWPDDLSWCVATEIDLFCTYVGGSRALIDELLGDERLEAWEAHLDDPVAHDS
                     DVLNG"
BASE COUNT          542 a         1056 c         1000 g          492 t
ORIGIN      
        1 tgcgtgttcg gcaccggccg caggcgccga gctggcgggc aagtgaagtc gtgggtgagg
       61 tcgagcgcgc cggtcgggat gcgacggacc gggatacgtc ggaccgggct gaggagggtg
      121 gactgcggac tcataacgac ctccggggaa gggaagctcg gcgcggatga tgacctcgaa
      181 cggatgttcg atcgaacgcc tgtacgaagt tctaccccat cccaccgaca aaaccacgac
      241 acgttcgaac agatgtttga atgcgtgtcg ggaaagggct acggtcaggc cagccgacca
      301 acacgatgaa ggggcgaggt catgtcgcca cgcgacgcag aaggtccagg gccgctcgcc
      361 ggcgtcagcg actttccgga tggtccgccc gatgagaccg gcctgacacc gcgccagcac
      421 cgggtcctcg acgtgatccg cgactccgtc gagcggcgtg gctacccgcc cagcatgcgc
      481 gagatcggcg agcaggtcgg gctgacctcc acgtccagcg tcgcccacca gctgacgact
      541 ctgcagaaga agggctacct gcgccgcgac ccaaatcgcc ctcgtgcggt cgaggtgcgg
      601 atcccgggcg agattgccgg cacctcgacg tccgagcccc gtggcacccg agagcacagt
      661 ggcaccagag aacacgcggc ccccttcgac gagaccgaga gcggcaactc ccgcccggca
      721 cccgcctaca tcccggtcgt cgggcggatc gccgccggtg gcccgatcct cgcggagcag
      781 gcagtcgacg acgtcttccc gctcccccgc aatgtcgttg gcgagggcac gctgttcatg
      841 ctgaaggtgg tcggcgattc gatggtcgac gcagcgatct gcgacggcga ctgggtcgtc
      901 gtccggcagc aaccggtcgc cgagagcggt gacatcgtcg cggcgatgat cgacggcgaa
      961 gccaccgtga agaccttcaa gcgacgcgac ggtcacgtct ggttgatgcc gcacaacccg
     1021 aactacgcgc cgattcccgg cgacgacgcg acgattctcg gccgcgtgag cgcggtcctg
     1081 cgcaaggtct gatcgccgcc acaggtgcgg cccggttagg gtcgtgatga tggacgacga
     1141 gcaggtgctg gccagcgacg gtgtcaccta cgtcgtacag ctcggtgaca ccgtgcggcg
     1201 cccggtgcgg ccgttcaccg cgaccatcca ggcctaccta gaacacctgc atgatcgcgg
     1261 cttccacgat gctccgatcc cgctgggcta cgacgcgcag ggccgcgagg tgctctcctt
     1321 cgtaaccggc gacgtgccgc aggaaccgct tcccgactac gcctgcaccg acgacgtcct
     1381 ggtagcgctc gggacgttga tccgacggct acacgatgca gctgccgact tcattccgcc
     1441 cgtcgatgca atctggggtg gcatccctgg cacgccagcc ggcgtcgtcc cgctgttcga
     1501 caatcccgag ctcgtctcgc accaggacta ctgcccgggc aacgtcgtct tccgcacagg
     1561 cctgcccgca gcgttcatcg acttcgacct cagccggccc acgacgcgcg tcgccgacct
     1621 cgtcaacgcc atgtactggt ggaccccgct catgcatcca agcgacagac caccagccat
     1681 gcgggacgtc gatgtgccgg cgcgcgcacg gatcctggcc gacgcgtatg gcatgaccgc
     1741 acagcagcgc gcactcgtcg tcccgaccgc ggtgcagcgt gccgccaacg cgatgcccgc
     1801 catgcacgca gcggcgcagg ccgaccccgt cttcgcgaag tggtgggacg gtggcctcga
     1861 gcagaggctg ctgcgggccc aagagtggct ggccaccgag gccccccgca tcgacaccgc
     1921 cctgcgcgac taacgtggag gcatctgatc gcgctttcaa ctgttgagtg gggcactatg
     1981 tcggaacaac acaccaagcc gtccgctgac gccttactcg ccgctgtgcg caactcccgc
     2041 gcgcggctgg tcgagaccgt gacatcgttg actcctgagc agctgcgcga acagtcgtac
     2101 gacaccgagt ggtcgatcgc tcaagtgctg tcgcacatcg ccaccaccgc ggtcttcttc
     2161 aagctcatcc tgcaggccgg cctcgatggc cagccggcac ctgcgatgga tctcatgcgc
     2221 ccggtctggg atgaatggaa cgcgaagagc gcgcaggagc aagcccacga cggattggca
     2281 gccgacggcg agtaccttgc cgcgctcgag tcgctgaccg cgcagcaacg cgccgactgg
     2341 cagctcgacc tattcggcac gcatcgcgac ttcgccgggc tactgaccat gcgggtaggc
     2401 gaacatgcgg tccacacgtg ggacgtcgtc gtggcactcg atccgtcggc gatgcttgct
     2461 cccgacgcag tcgacatcct catcgacacc ctcggacagc ttgtcgggtg ggtcggtaaa
     2521 ccgctcgatc agcaattggt catcggcgtc gagaccgaca atccgagccg caactttgtg
     2581 ctgaccgtcg acgctgaggg tgcgacgctc gagccgctcg atgcgcgctc tgatgcagat
     2641 cgcctgttgg cgctacccgc cgaggcattt gtccggctgg tctatggccg cctcgatccg
     2701 gagcacacgc cgtccgtggc ggacgatccg attctcgaag tactgcggcg cgtcttcgtc
     2761 ggtttctagc cgttcagcac gtcgctgtcg tgggcgaccg gatcgtcgag gtgcgcttcc
     2821 catgcctcga ggcgttcgtc gccgagcagc tcgtcgatca gggcgcgaga tccgccgacg
     2881 tatgtgcaga acaagtcgat ctcggtcgct acgcaccacg acaggtcgtc aggccagaag
     2941 atgtttggcg agtgcggcaa gaaccactcc ggggtcggcc agtcaccgat ctcgagcgca
     3001 cagtcgagcc gcccgccgaa cagcaggtag ttgcgtcgtg gcagctcaac gcgggcgtca
     3061 aagtcgattg ctgcgaaacg ccgatcagcc
//
LOCUS       DATCBJ010000346         3242 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_216246, whole genome shotgun sequence.
ACCESSION   DATCBJ010000346 DATCBJ010000000
VERSION     DATCBJ010000346.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 3242)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3242)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3242
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_216246"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..539)
                     /locus_tag="VII50_06885"
     CDS             complement(<1..539)
                     /locus_tag="VII50_06885"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: protein motif:HMM:NF006089.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase subunit sigma-70"
                     /protein_id="HEY5050609.1"
                     /translation="MTTTRAMPQELEDRFKQSRIELRGFCYRMLASPFDAEDAVQETF
                     IRAWRGYDSFEGRASLRSWLYKIATNVCLDMRRSAERRARPMDFGPSQEPIAANLNVL
                     PETTWIEPVPDELIVAADRDPADVAVARDSVRLAFVAALQHLPPRQRAVLILCEVLQW
                     KATEVAELLETTTASVNSA"
     gene            710..1432
                     /locus_tag="VII50_06890"
     CDS             710..1432
                     /locus_tag="VII50_06890"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY5050610.1"
                     /translation="MTSDVARSRAFYCELFGWTADEPNPDFGGYVNFRKDGVLVAGGM
                     ARDSGNPMPDTWSVYLATDDAAKTLETAIAHEGRVMIEPMQVGELGTMAAIIDPGGAM
                     IGVWKPGLHKGFGVLGEPGAPSWFELHTRDYDAAVAFYRDVFHWDTQTVSDTAEFRYT
                     VQQYGAAQLAGVIDASTFPPDGVPAHWGVYFGVADTDVAVATTLALGGAIVRPAEDTP
                     YGRLAVLTDPTGAQFRLMSLSS"
     gene            1597..2268
                     /locus_tag="VII50_06895"
     CDS             1597..2268
                     /locus_tag="VII50_06895"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HEY5050611.1"
                     /translation="MTAQPEPQPSPTRTLWMMAGGEVTLLPVDLESGLQRGVVTHWLT
                     GAAGLVVTIGVRTSREAAAMCSGQRVWVSGREAADEELIVLEVIARRASEQDDTTLIL
                     TGVLPLARERRRSAVRAATRHPAMLTYTDGTTAQGVATDLSHGGCRIDLNQPDLAQSV
                     GAMAEVRIELPGNRRFALEAEVVRIDTQTGELGMRFDPTEIDLAPIDRLVYAAVKRQK
                     ISEEY"
     gene            2464..2856
                     /locus_tag="VII50_06900"
     CDS             2464..2856
                     /locus_tag="VII50_06900"
                     /inference="COORDINATES: protein motif:HMM:NF012610.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEY5050612.1"
                     /translation="MIEFHLNASSGVSPYLQLVQQVRRALVLGLLSASDQLPTVKDVV
                     SQLAINPNTVLKAYRELEHSGLVAARAGVGTFVISTLSDSSRAAHGPLREELVQWFGK
                     ARRAGLDDESIEALFQDTFRSLDAQGVA"
     gene            2853..3161
                     /locus_tag="VII50_06905"
     CDS             2853..3161
                     /locus_tag="VII50_06905"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY5050613.1"
                     /translation="MTAVLDASGLGKRYRQRWALRDCTLQVPAGRVVGLVGPNGAGKT
                     TLLHLAVGLLQPVPGRSESSVDDLPKVRRNSGGLVSSRRTRPSTPRFRWPIICGWAGG
                     "
BASE COUNT          566 a         1072 c         1054 g          550 t
ORIGIN      
        1 aaggcgctgt tgaccgacgc cgttgtcgtc tcgaggagtt cggcgacctc cgtcgccttc
       61 cactgcagca cctcgcacaa gatgaggact gcacgttggc ggggcggcag gtgttgcagc
      121 gcagcgacga aggcgagccg cacgctgtcg cgcgccaccg cgacgtccgc gggatcccgg
      181 tcggcggcaa cgatcagctc gtcaggcacc ggctcgatcc aggtcgtctc gggcagcacg
      241 ttcagatttg ccgcgatcgg ctcttgcgaa ggcccgaaat ccatcggccg cgcacggcgc
      301 tcggcgcttc ggcgcatgtc gaggcagaca ttggtcgcga tcttgtagag ccatgagcgc
      361 agcgacgcgc ggccctcgaa gctgtcgtat ccccgccacg cgcgaatgaa ggtctcctgc
      421 accgcatctt ccgcgtcgaa aggcgacgcc agcatgcgat agcagaaccc gcgcaattcg
      481 attcgggact gcttgaaccg atcttcgagc tcctgcggca tggcgcgggt tgtggtcatc
      541 gggacatgct aggcacccca gccgacaatg tgagatgtgc gctccgatga attcgcgagc
      601 tccgcgccgt ccactctcaa agtcttgacc gcgccacttc aacctcggga gagccgccat
      661 gcctgcacgc gaagatgccc ctatcggagc gccctgctgg atcgacctga tgacgtcaga
      721 cgtcgcgcgc agccgcgcgt tctattgcga gctgttcggt tggaccgcgg atgagcccaa
      781 tccggacttc ggcgggtacg tcaacttcag gaaagatggc gtcctggttg cgggcggtat
      841 ggcgcgcgac tctggcaatc cgatgcctga cacctggtcc gtgtatctcg ccactgacga
      901 tgctgcgaag acgttggaaa cggccatcgc ccatgaaggg cgggtcatga ttgagccgat
      961 gcaggtcggc gagctgggca cgatggccgc catcatcgac cccggcggcg cgatgatcgg
     1021 cgtctggaag cccggcctgc acaagggctt cggcgtgctc ggggagcccg gtgccccgag
     1081 ctggttcgag ttgcacaccc gcgactacga cgccgccgtg gccttctacc gcgacgtgtt
     1141 ccactgggac acgcagacag tcagcgatac cgcggagttc cggtacacgg tccagcaata
     1201 cggagcagcg cagctggccg gggtcatcga tgcctcgacg ttcccgccag atggcgttcc
     1261 cgcgcactgg ggcgtctact tcggagtcgc cgacaccgac gtcgcagtcg cgacgacgct
     1321 tgcgctgggc ggcgcgatcg tccggcctgc cgaggacacc ccctacgggc ggctggccgt
     1381 cttgactgac ccgaccggcg cgcaattccg cttgatgagc ctcagcagct gagaccaggc
     1441 agctgagacg ggcagccgaa cggtaccgat cggtgactgt tcggcatcta tacataaatg
     1501 caattcccag ctacacatga ttggtaggta ctctaaatag ggcgaatcgt ctgatttttc
     1561 gcagccgtca gctcacacgc agaccgggag cggacgatga ccgcgcaacc agagccgcaa
     1621 ccgtcaccca cccgcaccct ttggatgatg gccggcggcg aggtcacgct gctccccgtc
     1681 gacctcgaaa gcgggctcca acgtggggtc gtcacgcatt ggctcaccgg cgcggccggc
     1741 ctcgtcgtca ccatcggcgt ccgcacgagt cgcgaagccg ccgcgatgtg ctctggccag
     1801 cgggtgtggg tgtccggtcg ggaagccgcc gacgaggagc tcatcgtgct cgaggtcatt
     1861 gcccggcgag cctccgagca agacgacacg acgctgatcc ttaccggcgt cctgccgctc
     1921 gcccgcgaac gccgccgcag tgcagtacgc gcggcgacgc ggcatccggc catgctcacg
     1981 tacacagacg ggacgacggc gcagggcgtc gccacggatc tctcccacgg cggttgccgt
     2041 atcgacctca accagcccga cctcgcgcaa tcggtcggcg caatggctga agtacgcatc
     2101 gagctcccag gcaaccggcg gttcgcgctg gaagccgagg tcgtgcgcat cgatacgcag
     2161 accggcgagc taggcatgcg gttcgatccc accgagatcg acttggcacc gatcgaccgc
     2221 ctcgtctacg ccgcggtcaa acgacagaag atctcggagg agtactgacg gaactacccg
     2281 gcccgcgatc gtccgccggc cggctcgcac gcgaagttga tcacgttagg cagccctaga
     2341 ggtcccgagg taggcgacga cccctgcccc ggctacatct cggctgcccc aggccaggct
     2401 cttgacttgc ggcggctccc cagttaacta ttgtgctagt taactagtta attgggagtc
     2461 gctgtgatcg agttccacct gaacgcatcg tcgggggtct ccccctacct gcaactggtc
     2521 cagcaagtgc gacgtgcatt ggtgctgggc ttgctcagcg ccagcgacca gctgccaacc
     2581 gtgaaagacg tcgtgtcgca gctggcgatc aacccgaaca ccgtgctcaa ggcttaccgc
     2641 gagcttgagc acagcgggct tgtcgcggca cgcgcaggtg tcggcacctt cgtgatctcg
     2701 acactgagcg actcatcgcg tgcggcgcac ggtccgctgc gcgaggaact cgtgcagtgg
     2761 tttgggaagg cacgtcgggc cgggctcgac gatgagagca tcgaggccct gtttcaggac
     2821 acctttcgga gcctagacgc gcaaggcgtg gcatgaccgc ggtcctcgat gccagcggcc
     2881 tcggcaagcg ctaccgccag cgttgggcat tgcgggattg cacgctgcaa gtgccggccg
     2941 gacgcgtcgt gggcctcgtc ggaccgaacg gagcagggaa gacgacgctg ctgcaccttg
     3001 cggtcggctt gctgcaacca gtgccgggca gatcggagtc ctcggtggac gacctgccga
     3061 aagtccggcg caactcgggc gggttggttt cgtcgcgcag gacacgcccg tctactcctc
     3121 gctttcggtg gccgatcatc tgcggttggg caggtggctg accccccgct tcgatgcaga
     3181 agtcgccgaa cggcgcatcg atcagctcgg gctcgatcgc aagcagcgcg ccggcaaact
     3241 gt
//
LOCUS       DATCBJ010000347         4176 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_214539, whole genome shotgun sequence.
ACCESSION   DATCBJ010000347 DATCBJ010000000
VERSION     DATCBJ010000347.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 4176)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4176)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4176
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_214539"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..575
                     /locus_tag="VII50_06910"
     CDS             <1..575
                     /locus_tag="VII50_06910"
                     /inference="COORDINATES: protein motif:HMM:NF024771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HEY5050614.1"
                     /translation="AIRYISALEATFDLTWVEEPLRRWDAEGLAMVGRSVRAAIATGE
                     NLTGLEQFKPLLDASAVDVVQAGSVWGITHFLRVATVAHSRDLPISPVGYNTNPVAHA
                     AASIPNHLAIEIQSLETPVGLAVDQTIADGGVVLGDEPGNGLSVDEAMIASAEARLAW
                     SDPAGPHVRPHRAGLRLVPEESASDTVRMS"
     gene            575..1207
                     /gene="eda"
                     /locus_tag="VII50_06915"
     CDS             575..1207
                     /gene="eda"
                     /locus_tag="VII50_06915"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR01182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008675 -
                     2-dehydro-3-deoxy-phosphogluconate aldolase activity
                     [Evidence IEA];
                     GO_function: GO:0008700 - 4-hydroxy-2-oxoglutarate
                     aldolase activity [Evidence IEA];
                     GO_process: GO:0009084 - glutamine family amino acid
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009255 - Entner-Doudoroff pathway through
                     6-phosphogluconate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HEY5050615.1"
                     /translation="MALDQLRMHVVLPVVVADDRDCATPLGNALVAGGLPVAEVTLRT
                     PAALDVIRVLAQRADILVGAGTVLTADQVDAAVDAGAAFIVSPGLSRTVVDRCFEHRV
                     LPLPGAATATEVQAALELGLDTVKFFPAESAGGANAISALAAPFADVSFVPTGGINVS
                     NLDSYLALPSVVAVGGSWMIPRDLVRSHDVAKLIGLITSVTTQVKKSRPR"
     gene            1230..2330
                     /locus_tag="VII50_06920"
     CDS             1230..2330
                     /locus_tag="VII50_06920"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HEY5050616.1"
                     /translation="MLEQVLPPSDVRFDIVSLGEVMLRLDPGPGRIRTSRQFHVWEGG
                     GEYNVARGLGRVFGLRAGIVTALADNEVGRLVEELMLTGGVDTSFVQWVNYDGVGRAT
                     RNGLNFTERGFGVRGAVGVSDRGHTAVSQLTPGDIDWDRLFGTIGVRWFHTGGIFAGL
                     SPSAADTALQAVKAAKRHSTTVSYDLNYRPSLWASTGGQARAQAVNRAIAPYVDVILG
                     NEEDFTAALGFEVDTADADLTRLEVEKFATMIRNVAAEYPNLKVIATTLRRVRSATVN
                     DWGAVAWSPETDIVRAVDREHLEVFDRVGGGDSFASGLIYGLLSGESLQTAVDYGAAH
                     GALAMTTPGDASMMSKADVINLARGGSARVDR"
     gene            complement(2331..3326)
                     /locus_tag="VII50_06925"
     CDS             complement(2331..3326)
                     /locus_tag="VII50_06925"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEY5050617.1"
                     /translation="MTALPTRALGSTGLRLTELAFGAASLGNLYRVTSDEEASGAVAQ
                     AWECGMRYFDTAPHYGLGLSERRLGRALAGYPRNAYVVSTKVGRLLERNPYPTPVDTD
                     GFEVPGDLRRVWDFSRDGVRRSLDDSLRRLATDHVEIVYAHDPDQFRAGAARQALEAL
                     AQLRSQGVVRAIGVGTNVVDELAELFRDGLVDVVMLAGRYTLLEQGGLRTALEPALAA
                     GVGIVAVGVYNSGLLSVAWPASDAKYDYGQAPPDLIARARQLAVVCERHGTTLPDAAI
                     AFPLLHPAVSSVAIGMRTAEQVVSNVARYQRGVPGEVWADLLDSELIDPAAVKLG"
     gene            complement(3323..>4176)
                     /locus_tag="VII50_06930"
     CDS             complement(3323..>4176)
                     /locus_tag="VII50_06930"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEY5050618.1"
                     /translation="DVTRHLIDSHLHIWERARNPQNWIDPATMSAIDRDFGPDAAAQE
                     MSARQVDGCVVIQCVNTFSETLDLLAAADSVGAILGVVGWVDLQADVPAQLDRLRAAP
                     GGRHLVGVRHVTTIEADESWLSRADVARGLTSLASAGLPFDVVVQPSQLPVVKTLAQS
                     IGQATFVLDHLGNPPIASSNLARWSADLAALATCENVLAKVSGLITKDDWDHWTVDRL
                     RPVIDHALEAFGPRRLMFGSDWPLAELAGGYRPWKNAYLQLTDDLTPAEKASIDAESV
                     SRVYGLR"
BASE COUNT          736 a         1354 c         1339 g          747 t
ORIGIN      
        1 aggcgatccg ttacatctcc gcccttgaag ccaccttcga tctcacgtgg gtagaagagc
       61 cactaaggag atgggacgcc gagggcctcg cgatggttgg ccggtcggtc cgagccgcga
      121 tcgctacggg cgagaacctc accggactcg agcagttcaa gcccttgctt gatgcgagcg
      181 cagtcgacgt cgttcaggcc ggaagtgtct ggggtatcac tcattttctt cgggtcgcaa
      241 ccgtcgcaca cagccgcgac ttgccgatta gtcccgtcgg gtacaacacg aacccggtcg
      301 cccacgccgc ggcgtcgatc ccgaaccatc tcgcgatcga gatccaaagc ctggagactc
      361 ctgtcgggct ggcggtcgac cagactattg ctgacggcgg cgtggtcctg ggtgatgaac
      421 caggcaacgg actgtcggtc gatgaggcga tgatcgcaag tgccgaagcc cgcctggcat
      481 ggtcggatcc cgcgggcccg cacgtgcgcc cccatcgcgc cggtcttcga ctcgtccctg
      541 aggagtcggc ttccgacacc gtccgcatgt cgtgatggct ctcgatcaac tgcgaatgca
      601 cgtcgtcctg cccgtggtcg ttgccgacga ccgcgactgt gcgaccccgt taggaaatgc
      661 gctcgttgcg ggcggcttac cggttgccga agtgacgctc cgcacgccag ccgcattgga
      721 tgttattcgg gtgctggcac agcgagcaga catccttgtc ggcgcaggaa cggtactgac
      781 cgcagaccaa gttgatgccg ccgtcgacgc aggtgccgcg ttcatcgtgt caccggggct
      841 tagccgcacc gtcgtcgatc gatgcttcga gcaccgcgtg ctccctctac ctggtgcggc
      901 gactgccacg gaggtgcagg ctgcgctgga gctcggtttg gacacggtga agttcttccc
      961 agcggagtct gccggcgggg ccaacgcgat ttcggccctc gcggccccgt ttgcggacgt
     1021 gtcctttgtt ccgaccggcg gtatcaacgt ctccaatctt gacagctacc tcgccttgcc
     1081 cagcgtggtc gctgtcggtg ggtcgtggat gatcccacgc gaccttgttc gctcgcatga
     1141 cgttgccaag ctcatcggtc tgatcaccag cgtcactacc caggtcaaga agtcgcgtcc
     1201 gcgctgatcg acaccgaaag gactctcgaa tgcttgagca agtcctcccg ccaagcgacg
     1261 tccgattcga catcgtctcg ctgggcgaag tgatgttgcg actcgaccca ggcccgggtc
     1321 ggatacgcac gagtcgccag ttccacgtct gggaaggcgg cggcgagtac aacgtcgctc
     1381 gagggctagg ccgagtgttt gggcttcgcg ccgggatcgt caccgcgctc gcggacaacg
     1441 aggtcgggcg actagtcgag gaattgatgc tgaccggcgg agtcgacacc tccttcgtcc
     1501 agtgggtcaa ctacgacggc gtcgggcgcg cgacccgcaa tggcctcaac ttcaccgagc
     1561 gaggcttcgg tgtccgcggc gcagtcggtg tcagcgaccg cggccacacc gcagtctcgc
     1621 aactgacgcc gggtgacatc gactgggacc gcctgttcgg caccatcggt gtgcgctggt
     1681 tccacacggg agggatcttc gcagggctgt ccccgtccgc cgcggacacg gccttgcaag
     1741 ccgttaaagc cgccaaacgg cacagcacaa cagtctccta cgacctgaac taccgtccaa
     1801 gcctctgggc ctcgaccggc gggcaagccc gcgcgcaagc tgtcaatcga gccatcgccc
     1861 cgtacgtcga cgtgatactt ggcaacgagg aggacttcac tgcggctctc ggcttcgagg
     1921 tcgataccgc agatgccgat ctgactcgcc ttgaggtcga aaagttcgcc acaatgatca
     1981 gaaacgtcgc ggccgaatac cccaacctga aggtcatcgc cacgacgctt cgccgagttc
     2041 gtagcgctac ggtcaacgac tggggcgcgg ttgcctggtc gccggagacc gatatcgtcc
     2101 gcgcggtcga tcgcgaacat ctcgaggtct tcgatcgggt tggcggcggt gactcgttcg
     2161 cgtcagggtt gatctacggt ctgctgtcgg gagagtcgct gcagaccgcc gtcgactacg
     2221 gagcggcgca tggtgcgctc gccatgacga cgccgggtga tgcgtcgatg atgagcaagg
     2281 ccgacgtcat caaccttgct cgtggaggaa gcgcccgcgt cgatcggtaa tcacccgagc
     2341 ttgacggccg ccgggtcgat gagctccgag tcgagaaggt cggcccacac ctcgcccggg
     2401 acgccgcgct ggtagcgggc cacgttgctt acgacttgct cggcggtgcg catgccgatg
     2461 gcgacactcg agaccgccgg gtgcaggagg gggaaggcga tagctgcgtc gggaagtgtt
     2521 gtcccgtgcc gttcacagac aacagcgagt tgccgggcgc gagcgatcag gtctggtggg
     2581 gcttggccgt agtcgtactt cgcatcggat gcaggccagg caacgctgag gaggcccgag
     2641 ttgtacacac cgacggcaac aatgccgacc cctgctgcca gggctggttc cagtgccgtc
     2701 cgcagcccac cctgctcaag caacgtgtat ctgccggcaa gcatcacgac gtcgaccaga
     2761 ccgtcgcgaa agagttctgc cagctcgtcg acgacgttcg tgccgacgcc gatcgcacgc
     2821 accactccct gcgaccgaag ctgcgcgagc gcctcgagcg cctgtcgtgc cgccccagcg
     2881 cggaactgat ccgggtcatg ggcgtagacg atttcgacat ggtctgttgc caagcgccgc
     2941 agggagtcgt cgagcgagcg acgcacgcca tcacgcgaga agtcccatac tcgtcgcaga
     3001 tcgcccggta cctcgaagcc gtcggtgtca accggcgtcg ggtacggatt gcgttccagc
     3061 agccggccca ccttggtaga gacgacgtat gcgttgcgcg gatatccggc taacgcgcgt
     3121 ccgagccgac gctccgacaa gccgagcccg tagtgcggcg cggtatcgaa ataccgcatc
     3181 ccacattccc acgcctgcgc aacggcgccc gacgcctcct catccgacgt tacgcgatag
     3241 aggttgccaa gactggccgc tccaaaagcg agctcggtca gccgaagacc ggttgaaccg
     3301 agggctcgtg tcggaagcgc ggtcatcgga gcccgtacac gcggctcacg ctctcggcgt
     3361 cgatggatgc tttctcggct ggcgtgagat cgtcagtgag ctgcaggtac gcgttcttcc
     3421 acggccgata cccacccgcc agttcggcca gtggccagtc cgaaccgaac atcagccggc
     3481 gaggcccgaa ggcttcgagg gcgtggtcga tgacgggtcg cagtcggtca actgtccaat
     3541 gatcccagtc gtcctttgta atcaagccgg ataccttcgc gagtacgttc tcgcacgtcg
     3601 cgagtgcggc aaggtcagcc gaccatcgag ccaaatttga gctggctatc ggggggttcc
     3661 ccaaatggtc gaggacgaac gttgcctgcc cgatcgactg ggccaacgtc ttgaccacgg
     3721 gcagctgcga tggctggacg acgacatcga acggcagtcc tgcgcttgct agggaggtga
     3781 gccctcgcgc aacatcggcg cggcttagcc agctttcgtc cgcttcgatg gtcgtcacgt
     3841 gccggacacc gacgaggtgc cgaccgccgg gcgccgctcg caacctgtcg agttgagcgg
     3901 ggacgtctgc ctggaggtcg acccagccga cgacaccgag aatcgcgccg accgagtcag
     3961 cggccgcaag caagtcgagt gtctcgctga acgtattcac gcactggatg acgacgcatc
     4021 cgtcgacctg cctggcgctc atttcctgtg cggccgcgtc cggcccgaag tcgcggtcga
     4081 tcgccgacat ggtcgcaggg tcaatccagt tctgcgggtt ccgcgcgcgc tcccatatat
     4141 gaagatgaga atcgatcagg tgccgcgtca cgtcag
//
LOCUS       DATCBJ010000348         2545 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_219610, whole genome shotgun sequence.
ACCESSION   DATCBJ010000348 DATCBJ010000000
VERSION     DATCBJ010000348.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2545)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2545)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2545
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_219610"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            44..244
                     /locus_tag="VII50_06935"
     CDS             44..244
                     /locus_tag="VII50_06935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006243848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HEY5050619.1"
                     /translation="MTQGVVKWFNAEKGFGFIAVEGGADVFVHYSAIQSDGYRSLEEN
                     QKVDFDITQGPKGPQAENVRPV"
     gene            361..1140
                     /locus_tag="VII50_06940"
     CDS             361..1140
                     /locus_tag="VII50_06940"
                     /inference="COORDINATES: protein motif:HMM:NF012401.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HEY5050620.1"
                     /translation="MNRPWDDDERPPDPPLDDTAAEVDGAGRPIGRRIVLGMLGLGAV
                     GVIFGKQLENVQSNILDPVVRNDPTGLSNLLPAVDRFRYYNVASSEPNRTIADYQLTV
                     GGLVDRPMTLSYADLQALPQTDLIKDFQCVTGWRVPQVHWSGVALPDLLDHVGVASGA
                     TAVQFTSFDGVYTESLTLAQAQRRDIIVALTMLGGPVTRDHGGPVRLYVAPMYGYKSC
                     KWLGGIKLANHVTPGYWEDEGYDVDAWVGKSNGRGDDAPTS"
     gene            1152..1781
                     /locus_tag="VII50_06945"
     CDS             1152..1781
                     /locus_tag="VII50_06945"
                     /inference="COORDINATES: protein motif:HMM:NF013460.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b/b6 domain-containing protein"
                     /protein_id="HEY5050621.1"
                     /translation="MRPPAELLRFTRGERWVHRATAILMGICLATAAILYIDVLSQLF
                     GHRALVEWIHVIAGIGLPIPIILGLLSKAFRADAGRLNRFTRNDWKWLRSRQGRNGTI
                     PVGKFNAGQKLNANFQLGAIFTMLGTGLIMRFANVWPVSLRTGATFVHDWMSYAILAM
                     VLGHMYMANRDPHALRGMRTGYVPTSWARREHKAWAVREVERTKATKSS"
     gene            1805..>2545
                     /locus_tag="VII50_06950"
     CDS             1805..>2545
                     /locus_tag="VII50_06950"
                     /inference="COORDINATES: protein motif:HMM:NF021371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2071 domain-containing protein"
                     /protein_id="HEY5050622.1"
                     /translation="MPSPSDVGAYGPVCPLDVPHPVMMHRWDTLTFLHWSYEPDVVAR
                     LLPVGLTLDTFDGKAWVGLVPFNMLVSLPHTPSLPWASRFCETNVRTYVIDEYGRPGI
                     WFFSLDASRLGAVLTARATFRLPYMWSRMSMVRDGDVVTYSCSRRWPGPRGASSRVII
                     DIGPPFKPTELTQHDHFLTARWQLFSVTGPGMFRGFAATASDRAGAGSWRRYGIAQHD
                     PWVLHRATARFVDDGLVAAAGLPQPVGEP"
BASE COUNT          424 a          833 c          823 g          465 t
ORIGIN      
        1 tgaccgcgag gtgcggggac cgaatcctgg ttagggagag cacgtgacgc aaggtgtagt
       61 gaagtggttc aacgctgaga agggcttcgg cttcatcgcc gtcgagggcg gcgcagacgt
      121 cttcgtccac tactcggcca ttcagtccga cggttaccgc agcctcgaag agaaccagaa
      181 agtcgacttc gacatcacgc agggcccgaa aggcccgcag gcggagaacg tccgccccgt
      241 ctaagttcgc agcgtccggg tggtccgcac cgagaggtgt ggccacccgt tcgtgtttcg
      301 taatggcttt cggcgcagtt agcggcgtcg cggcgcaact cgtgagcgca tcatagaagg
      361 atgaaccgcc cgtgggacga cgacgagcgg ccgccagacc cacccctgga cgacaccgct
      421 gctgaggtcg atggagccgg tcgaccaatc ggccgccgca tcgtgctggg catgctcgga
      481 ctgggtgccg ttggagtgat cttcggcaag cagctcgaga acgtccaaag caacatcctg
      541 gacccggtcg tccgcaatga ccccacgggc ctgtcgaacc tgctgcccgc cgtcgaccgg
      601 ttccgctact acaacgtcgc gtcctccgaa ccgaaccgca cgatcgccga ctaccagctc
      661 acggttggtg gcctcgtcga tcggccgatg acgttgagct acgccgatct ccaggcgttg
      721 ccgcagaccg acctcatcaa ggacttccag tgtgtgaccg ggtggcgggt gccgcaggtg
      781 cactggtcgg gagtcgcctt gcccgacctg ctggaccacg tcggcgtcgc gagcggcgcc
      841 accgcagtcc agttcacgtc gttcgacggg gtctacaccg aaagcctcac cctcgcgcag
      901 gcgcaacggc gcgacatcat cgtcgcgctg acgatgctcg gcgggcccgt cactcgtgac
      961 cacggcggac cggtccggct ctacgtcgcc cccatgtacg gctacaagtc gtgcaagtgg
     1021 ttgggcggca tcaagctggc caaccacgta acgccggggt actgggagga cgagggctac
     1081 gacgtcgacg cgtgggtcgg caaatcgaac ggtcgcggcg acgatgctcc gacgagctga
     1141 ccgtgacgaa gatgcgcccg cccgctgagt tgctgcgctt cacccgcggc gaacgctggg
     1201 tgcatcgcgc gacggctatc ctgatgggca tttgccttgc gactgctgcg atcctctaca
     1261 tcgacgtgct ttcgcagttg ttcgggcacc gcgcgctcgt cgaatggatc cacgtcattg
     1321 ctggcatcgg cttgccgatt ccaatcattc ttggcctgct atcaaaggct tttcgcgccg
     1381 acgccggccg gctcaaccgg ttcacccgca acgactggaa gtggctgcgg tcgcgtcaag
     1441 gtcgtaacgg caccatcccg gtcggcaagt tcaacgccgg ccagaagctc aacgcgaact
     1501 tccagctcgg cgccatcttc acgatgctcg gcaccggact catcatgcgc tttgcgaacg
     1561 tctggccggt ctcgcttcgt accggcgcca cgttcgtgca cgactggatg tcgtacgcaa
     1621 ttctggccat ggtgctcggc cacatgtaca tggccaaccg cgatccgcac gcgttgcgcg
     1681 gcatgcggac cgggtacgtt ccgacctcat gggcgcgacg cgagcacaaa gcctgggccg
     1741 tgcgcgaagt cgagcggacc aaggcgacga agagcagctg atcggcgtcc gacattatgg
     1801 gtttatgcct agcccgtcgg atgtcggagc ctacggcccg gtgtgcccgc tcgacgtccc
     1861 gcacccggtg atgatgcacc gctgggacac gttgacgttc ttgcattggt cctatgagcc
     1921 ggacgtcgtc gcgcggctgc tacccgtcgg gctcacgctc gacacgttcg atggaaaggc
     1981 atgggtcggg ctggtcccgt tcaacatgct cgtgagcctg ccgcacacgc cgtcgctgcc
     2041 atgggcgtcg cgcttttgcg aaaccaacgt gcggacctac gtgatcgacg agtacggccg
     2101 gccgggcatc tggttcttct cactcgacgc gtcgcggctc ggcgcggtgc tgacggcgcg
     2161 tgcgactttt cggctgccgt acatgtggtc gcgcatgtct atggtgcgtg acggcgacgt
     2221 ggtcacgtat tcctgttcac gtcgctggcc gggtccccgt ggcgcgagta gccgcgtgat
     2281 catcgatatc gggccgccgt tcaaaccaac cgaactcacc cagcacgacc atttcctgac
     2341 tgctcgctgg cagttgttca gtgtcaccgg accgggcatg tttcgcggct ttgcagcgac
     2401 ggcgagcgac cgcgcgggtg ccgggagctg gcggcggtac ggcatcgcgc agcacgatcc
     2461 ctgggtcttg caccgcgcaa ccgctcgctt cgtcgatgac ggattggtgg cagcggccgg
     2521 acttccgcag cccgtcggcg aaccg
//
LOCUS       DATCBJ010000349         4796 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_120131, whole genome shotgun sequence.
ACCESSION   DATCBJ010000349 DATCBJ010000000
VERSION     DATCBJ010000349.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 4796)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4796)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4796
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_120131"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..692)
                     /locus_tag="VII50_06955"
     CDS             complement(<1..692)
                     /locus_tag="VII50_06955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009078614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO1664 family protein"
                     /protein_id="HEY5050623.1"
                     /translation="MTEQSFELSAADVRTVLTEGDLAIEGRLVDASNTTLYAVVTHSA
                     VSLTCVYKPIAGERPLWDFPDGTLAGREVGAYLVSEATGWDVVPPTVMRDGPLGPGAC
                     QLWIDIDEDADVMALLHSNHPGLRRMAVFDAVVNNADRKGGHLLPTPGGHIYGVDHGV
                     CFAAEDKLRTVLWQWRGTALPDDALDTLAALRIDLASDLGTELKALITPREVSAIRRR
                     VDRLLREGLFPMP"
     gene            complement(703..1299)
                     /locus_tag="VII50_06960"
     CDS             complement(703..1299)
                     /locus_tag="VII50_06960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3090 domain-containing protein"
                     /protein_id="HEY5050624.1"
                     /translation="MPRHIFLYDPPDRLVIGTVGQPGERTFFMQARSTGQLTSVVLEK
                     AQVAALAERLDQLLDEVLRASGGTSSVPAVAPLELNDTLPLDQPIVEEFRVGALGLGW
                     DPETERVIVEALAISDVVGDGTDDDSDDSTDPALLSDDDAEGPDLLRVRITGGVARAF
                     TKRALAIVAAGRPPCQFCGQPLDPESHVCARSNGHHPH"
     gene            complement(1310..2011)
                     /locus_tag="VII50_06965"
     CDS             complement(1310..2011)
                     /locus_tag="VII50_06965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020659422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MSMEG_4193 family putative phosphomutase"
                     /protein_id="HEY5050625.1"
                     /translation="MTTVLLVRHGLTALTGPVLAGRTPGVDLDDRGRAQAAAVAQRLA
                     GVAIQAIVSSPLERCLQTAQLIAQDRDVAVETDVRLIECGYGDWTGQEINKLAKDPLW
                     KVVQAHPSAVTFPGEGGEAMRDAQVRAVDAVRSCNAKLGADATWLACSHADIIKAVVA
                     DALGMHLDTFQRIVIDPCSITVISYTSLRPFVVRVNDTGTDLTTLLAPPAKGRRRRAK
                     TAGSDAVVGGGAGAK"
     gene            complement(2024..2260)
                     /locus_tag="VII50_06970"
     CDS             complement(2024..2260)
                     /locus_tag="VII50_06970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5050626.1"
                     /translation="MSAKSRTDRPLSHVQFVAIVVVLAISGVLALVTLAQVAGPHGKC
                     PSETQVSRPGASICWAAYNNKLEPGVVGHPASTG"
     gene            2457..3383
                     /locus_tag="VII50_06975"
     CDS             2457..3383
                     /locus_tag="VII50_06975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009085180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY5050627.1"
                     /translation="MIEVRDLSTHFGSTVAVDHLTFSVRPGLVTGFLGPNGSGKSTTM
                     RMILGLDHPSKGGTTVNGRPYAAIRRPLHEVGALLDAKAMHRGRTAYNHLLCVARSNG
                     ISRRRVGECLDLVGLDTVGRKQARTYSLGMGQRLGIATALLGDPQVLMFDEPINGLDP
                     EGILWIRTLLRNLASQGRTVFVSSHLMSEMALTADHLVVIGRGRLIADSSTTDLIARS
                     SQGYVLVRTPDVEGLATRLRTAGAVVDTAADTMRVMGLECAAIGELAALDHIILHELT
                     PRSASLEEAFMELTRDSVDFCTDVTAANGRAS"
     gene            3380..3763
                     /locus_tag="VII50_06980"
     CDS             3380..3763
                     /locus_tag="VII50_06980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5050628.1"
                     /translation="MTDTATAAAESTHAYSPRTIGLTNVVRSECTKLRTVRSTYWTAI
                     AAVVATIGVAVLGATEYVRNFDPKNLEGFDAANFVLQGLYVAQIALGALGVLTITGEY
                     ATGMIRTTLTAVPQRRVVIALKGLV"
     gene            3987..4202
                     /locus_tag="VII50_06985"
     CDS             3987..4202
                     /locus_tag="VII50_06985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5050629.1"
                     /translation="MLFLPSALIDLLPNSWRADAINFAPANAGTQILNTNRVHDMFGP
                     WQGLGMLTLYGLVILLGALYLLGRRDA"
     gene            4223..>4796
                     /locus_tag="VII50_06990"
     CDS             4223..>4796
                     /locus_tag="VII50_06990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEY5050630.1"
                     /translation="MEQRRLGTTGLTVSCIGLGTAMWGRDTDEHEASDQLKAFVDAGG
                     SLLDTADTYAAGDSERVIGMLLRKVVDRSDIVVASKAGGLLPAARPDGRRRDMSRRHL
                     LEALDDSLDRLGIDHVDLWQLQGWDSSTPLDETLSALDFAVVSGRARYAGVSNYSGWQ
                     TAKAASWQSWWPGRVPLASTQVEYSLLERGV"
BASE COUNT          781 a         1610 c         1587 g          818 t
ORIGIN      
        1 ggcatcggga agagaccttc gcgcagcagc cgatcgaccc ggcgccggat tgcactgacc
       61 tcccgcgggg tgatgagcgc cttgagttcg gtgccgagat cgctcgcaag atcgatacgc
      121 aaagcggcca aggtgtcgag cgcgtcgtca ggcagcgcgg tgccgcgcca ctgccacaag
      181 acggttcgta gcttgtcctc ggcggcaaaa cacacaccgt ggtccacgcc gtagatatga
      241 ccgcccggtg tgggaagcag gtgaccgccc ttgcggtcgg cgttgttgac caccgcgtca
      301 aacaccgcca tccgccggag acctgggtgg ttgctgtgca acaacgccat cacatcggcg
      361 tcctcgtcaa tgtctatcca gagttggcat gcacccgggc caagcggtcc gtcgcgcatg
      421 accgtcggcg gcacgacgtc ccacccggtt gcctctgaca cgaggtaggc cccgacctcg
      481 cgcccggcca gcgtgccgtc cgggaaatcc cacagtgggc gctcgcccgc gatgggcttg
      541 tagacgcacg tgagcgagac cgcactgtgc gtgaccaccg cgtacagcgt cgtgttggat
      601 gcatcgacga gccggccttc gatggcgaga tcgccctcgg tcaagacggt ccgaacatct
      661 gctgcgctga gctcgaagct ctgctcggtc acggcgatcc cgtcagtgcg ggtgatggcc
      721 gttggaacgc gcgcacacgt gactctcggg atccagtggc tgaccgcaga actggcacgg
      781 cggccggccg gcggcgacaa tcgccagcgc gcgtttcgtg aacgcgcgcg cgaccccacc
      841 cgtgatgcgc acccgcagca agtccggacc ttcggcgtca tcgtcgctca gcaaagcggg
      901 gtcggtcgaa tcatcactgt catcgtcggt gccgtccccg acgacgtcgc tgatcgcgag
      961 cgcctcgacg atcacccgct cagtctcagg atcccagccg agcccgagcg ccccgacccg
     1021 gaactcctcc acgatcggct ggtcgagggg cagggtgtcg ttgagttcga ggggcgcgac
     1081 cgcgggcacc gacgacgtcc cgcctgatgc gcgcaagacc tcgtcaagga gctggtcgag
     1141 ccgctccgcg agcgcggcga cctgcgcctt ctccagcact acgctggtca gctgacccgt
     1201 gctgcgtgcc tgcatgaaaa aggtccgctc gccgggctgc ccgacggtac cgatgaccag
     1261 gcggtcgggc gggtcgtaca gaaagatgtg acggggcata gcgacgagct tatttggcgc
     1321 ccgcgccgcc gccgaccacg gcgtcgctgc cggccgtctt cgcacgcctc ctccggccct
     1381 tcgccggcgg cgcgagcaag gtggtcagat ctgttccggt gtcattcacg cgcacaacaa
     1441 acggccgcag cgatgtgtag ctgatgaccg ttattgagca gggatcgatc acgattcgct
     1501 ggaacgtatc gaggtgcata ccgagggcgt cggctaccac ggccttgatg atgtcggcgt
     1561 gcgagcaggc gagccacgtc gcgtccgcgc cgagcttggc gttgcacgag cgcaccgcat
     1621 cgaccgcgcg gacctgcgcg tcacgcatcg cctcgccgcc ttcgcccgga aacgtcaccg
     1681 cgctcggatg tgcctgcacc accttccaca gcggatcttt ggccagcttg ttgatctcct
     1741 ggccggtcca gtcgccatag ccgcactcga tcagtcgcac gtcggtttcc accgcgacgt
     1801 cacggtcctg cgcgatcaat tgcgcggtct gcagacagcg ctccaacggg ctgctgacga
     1861 tggcctggat ggcgaccccg gcaagtcgct gcgccactgc ggcggcttgg gcgcgaccac
     1921 ggtcgtcgag atcgacacca ggcgtccggc cagcgagcac cggaccggtc aacgcggtga
     1981 gtccgtggcg aacgagcaga accgtggtca catcgcgccg gggtcagcca gtggaagctg
     2041 ggtggccgac gacgccaggc tcgagcttgt tgttgtaagc cgcccagcaa atcgacgcgc
     2101 ccggtcgcga gacctgggtc tcgctcgggc attttccgtg cgggccggca acctgggcca
     2161 aggtgacgag cgcgagcacc cccgagatcg ccagcacgac gacgatggcg acgaactgca
     2221 catgcgagag cggccggtca gttctcgact tcgcagacac ccgcaactcc tgcctggttc
     2281 gggaccccga tccggtgagt ccgccgcggc ccgagtgtat cgaggcgacg agtggttccc
     2341 tacgcggcac cacgggtcgc ttcagggtcg ggcccggaat ctcccggatc gtcggcgacg
     2401 aggccgccca gcatcgtgag acaggtcggc ggcaccccga aacgaaagca ggaccggtga
     2461 tcgaggttcg cgatctcagc acgcacttcg gctcgacggt ggcggtcgat cacctgacgt
     2521 tctcggtccg gcccggcttg gtcaccgggt tcctcggacc gaacggctcc ggcaagtcga
     2581 ccacgatgcg gatgatcttg ggacttgacc acccttcgaa gggcgggacg acggtcaacg
     2641 gccgcccgta cgccgccatc cgccggccct tgcacgaggt gggtgctctg ctggacgcca
     2701 aggccatgca ccgcggccgc actgcctaca accacctgct gtgcgtggcg cggagcaacg
     2761 ggatctcgcg gcggcgggtt ggcgaatgcc ttgacctcgt gggcctggac acagtgggtc
     2821 ggaagcaagc tcgcacctac tcgctcggca tgggccagcg gctcggcatc gccacagcat
     2881 tgctcggcga cccgcaggtg ctgatgttcg acgagccgat caacggcctg gatcccgagg
     2941 ggatcctctg gatacgcacc ctgctccgga atcttgcatc gcagggacgc acggtgttcg
     3001 tgtcgagtca cctgatgagc gaaatggcgt tgaccgccga ccatctcgtc gtcatcggtc
     3061 gcggtcggct catcgccgac tccagcacga ccgatctgat cgcccgcagc tcgcagggct
     3121 acgtgctcgt ccgcacgcca gatgtcgagg gacttgccac gcggttgcga accgcgggag
     3181 cagtcgtcga tacggccgcc gacaccatgc gggtgatggg tcttgagtgc gcggccatcg
     3241 gcgaactggc ggcactggat cacatcatcc tgcatgagtt gacgccgcgc tcggcgtcgt
     3301 tggaggaagc cttcatggag ttgaccaggg acagcgtcga tttctgcacc gacgtgacgg
     3361 ctgcgaacgg gcgcgcgtca tgacggacac cgcgaccgcc gcagccgaat cgactcacgc
     3421 atactcgccc cgcaccatcg ggttgaccaa cgtcgtgcgt tcggaatgca cgaagctgcg
     3481 cacagtgcgg tcgacgtact ggacggcgat tgctgccgtc gttgcgacga ttggcgtcgc
     3541 ggtgctgggt gcaacggagt acgtccgcaa cttcgacccc aagaacctcg aaggattcga
     3601 cgccgccaac ttcgtcttgc agggcctcta tgttgcacag atcgcgctcg gcgcacttgg
     3661 tgttctgacc atcaccggcg agtacgccac cggcatgatc cggacgaccc tcaccgccgt
     3721 cccgcaacgt cgtgtcgtta tcgcactcaa ggggcttgtg tagatcgtgg cgacggtgat
     3781 cgtgggcgag atcctgtcgt tcgctgcgtt cgggatcggg caggcgatcc tgcacatcaa
     3841 gcacgctggc gtctcgctgt cggatcccgg tgtgtcgcgt gtcgtggtcg gcggcggtct
     3901 gtacctcgcg tcggtcggcc tgctcgcctt tgccctcggg accctagtgc gcagaacccg
     3961 caggagccct ggcgacattc ttcggcgtgc tgttcctccc gtcggcgctc atcgacctac
     4021 tgcccaactc gtggcgcgcc gatgccatca atttcgcccc ggcgaacgcc ggcacccaga
     4081 tcctcaacac caaccgcgtc cacgacatgt tcgggccctg gcaggggctc gggatgctca
     4141 ccctgtatgg gttggtcatc cttctcggcg cgctgtacct ccttggccgt cgcgacgcat
     4201 gatcgcccga tacggtgctg ccatggagca gcggcgcctc gggacgacgg gcctgacggt
     4261 ctcgtgcatc ggcctcggta cggcgatgtg gggccgcgat acggacgagc atgaagcgtc
     4321 cgaccagctg aaggcgtttg tcgacgcggg tgggtcgctc ctcgacacgg ccgacacgta
     4381 cgcggccggc gacagcgagc gcgtcatcgg catgttgctg agaaaggtcg tcgatcgaag
     4441 cgacatcgtg gttgccagca aagccggcgg gctgttgccg gctgcacggc ccgacggtag
     4501 gcgccgcgac atgtcccgtc gccacctctt ggaggcactc gatgattccc tcgaccggct
     4561 cggcatcgac catgtcgatc tgtggcagct ccagggctgg gattcgtcga cgccgctcga
     4621 tgagacattg tcggcgctcg atttcgccgt cgtttcagga cgtgcccggt atgcgggtgt
     4681 gtcgaactac agcggctggc agaccgcgaa ggccgcgagc tggcagagct ggtggcccgg
     4741 tcgggtaccc ctcgcctcga cgcaggtcga gtactcattg ctcgagcggg gcgtcg
//
LOCUS       DATCBJ010000350         2740 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669 k141_643,
            whole genome shotgun sequence.
ACCESSION   DATCBJ010000350 DATCBJ010000000
VERSION     DATCBJ010000350.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2740)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2740)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2740
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_643"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1678)
                     /locus_tag="VII50_06995"
     CDS             complement(<1..1678)
                     /locus_tag="VII50_06995"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5050631.1"
                     /translation="MACLAGGVVLAMMVGSQEGSQADYFYGFKRSVLEPRIFVFLGIG
                     VLMFFAITYWPSIVPYLTRPGFWPLLSGALAVLAALELMHWYDPAGDGKFSSTATIVS
                     QTSNISALASAFFAWLAWTQLIVVLVAGAASIILRLRWLAFAVAAVAAIGAVISYLAH
                     QAVDNAGRPHVDHSLGYAGAMVGYLVIAGAMLASATTVEEHAQSKVFVGRLLGFRPGL
                     PLVIVGFILTLLAFVDAAWFSPGNKNTTLSQTHGLFSGSGLTSIAFAYLGYLGWVLLA
                     ICTVLAAAAAWLRNRLIGWVGAAAGTVGAILTLVTLYEISKLGAHDKVDAATGPWQNL
                     GAGGWMASAGLFLLGAAGFVAATAPRLSNLDARTVARYVSSDQVGTSTALGTRPAATA
                     GKSSKYLSAPGAARSAMFIAIAAALFYPPTATPFWQQVVVSEIGISMLLAVGLNVVVG
                     WAGLLDLGFIAFYAIGSYTTAYLVGSLPIKPPHWLIMTPLLTLPFAVGACLIAGLALG
                     APTLRLRGDYLAIVTLGFGEIIHLAAVNSQGITNGSEGLTNDTQRHTKSVP"
     gene            complement(1762..>2740)
                     /locus_tag="VII50_07000"
     CDS             complement(1762..>2740)
                     /locus_tag="VII50_07000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEY5050632.1"
                     /translation="AHCRRSDALPEGHPGMTQFFDYLILGLTRGSMYALIALGYTLVY
                     GVLQLINFAHSEVFMSGAFGSYIVVHLIVGDGSGTATWEVPIVIAVGMVTGALTAACV
                     AWTLERVAYRPLRRRGAPKLAFLIGAIGASFFLSTLASKLFNSQTTNQFPTFFDHNTT
                     LFKIDHANVKALQAVIVISAIVMMVFLDRLVRLTKLGRSIRAVSEDAPTAALMGIDID
                     KTISRTFIIGGLLGGAAGFLFGLNFSFAYNMGFAPGVKAFAAAVLGGIGNIRGAMLGG
                     LLLGVVENLVLLIPGFDASWTDVVAFIVLVGVLMFRPTGLLGERVGRAA"
BASE COUNT          588 a          855 c          883 g          414 t
ORIGIN      
        1 tcggcaccga cttggtgtgc cgctgcgtgt cgttggtcag gccctcgctg ccattggtga
       61 tgccctggga gttcaccgcc gcgaggtgaa tgatctcgcc gaaaccgagg gtgacgatcg
      121 cgagatagtc cccacgcagc cgcaacgtcg gtgcccctag cgcgaggccg gcaatcagac
      181 aggcacccac ggcgaagggg agcgtcagca acggggtcat gatcaaccag tgcggcggct
      241 tgatcggcaa agacccgaca aggtaggcgg tcgtgtacga gccgatggcg tagaaggcga
      301 tgaacccgag gtcgagcaga ccggcccagc cgacgaccac gttgaggcca acggccaaca
      361 acatcgaaat tccgatttcg gaaaccacga cctgctgcca gaacggcgtg gcggtcggcg
      421 gatagaacaa cgccgccgcg atcgcgatga acatcgcgga tcgagcagcc cctggtgcgc
      481 tcagatactt cgacgatttc ccggcggtgg ccgcgggccg ggtgccgagt gccgtggagg
      541 ttccaacctg gtccgagctg acgtaccggg caacggtgcg ggcatcgagg ttgctcaggc
      601 gcggtgcggt tgcggcaacg aaaccggccg caccaagcag gaaaaggccg gcgctggcca
      661 tccaaccgcc cgcgcctaag ttctgccatg gcccagtcgc agcatcgacc ttgtcgtgcg
      721 ctccgagctt gctgatctcg tacagcgtga ccagtgtgag aatcgcgccg actgtgcctg
      781 cggccgcgcc cacccagccg atcagtcgat tgcgcagcca agcggccgcg gcggccagca
      841 ccgtacagat cgccagcagt acccagccca agtagccgag gtacgcgaag gcgatcgagg
      901 tcagtccgct tcccgagaac agaccatgtg tctgcgagag cgtcgtgttc ttgttccccg
      961 gtgagaacca cgcggcgtcg acgaaagcaa gcaacgtgag aatgaagcca acgataacca
     1021 gcggcaggcc gggccgaaat cccagcaggc ggccgacgaa caccttgctc tgcgcatgtt
     1081 cttcgacggt ggtcgccgat gcgagcatcg caccggcaat cacgaggtag ccgaccatcg
     1141 cgcccgcgta cccgagcgag tgatcgacgt gtggacggcc ggcgttgtcg accgcctggt
     1201 gtgcgaggta gctgatgacg gcgcctatgg ccgcaacggc agcgaccgca aatgcaagcc
     1261 aacgcaggcg aaggatgatc gaggcggcac cggcaacgag gacgacaatc agttgggtcc
     1321 atgccagcca cgcgaagaac gccgaagcca gcgccgagat attgcttgtc tgactgacga
     1381 tggtggccgt tgaactgaac ttgccgtcac cggcggggtc gtaccaatgc atcaactcga
     1441 gagccgcaag caccgcgagc gcgcctgaca gcaacggcca aaagcccggc cgcgtcaggt
     1501 aggggacaat gctcggccaa taggtgatgg cgaagaacat cagaacgccg atgcccagaa
     1561 acacgaatat tcgcggctct agaacggatc tcttgaaccc gtagaagtag tcggcttgcg
     1621 agccttcttg cgacccgacc atcatcgcaa gaacgacacc gccggcaagg caagccacga
     1681 gacgcttcaa cagaacggtt cgtggcgatg gtgcgtggat caccgcccgg ggcggcagcg
     1741 tggcgggtgc actcaggccg gtcatgccgc ccgccccact cgctcgccga gaagaccagt
     1801 cggccggaac atcagcacgc cgacgagaac gatgaaggcg acgacgtcgg tccagcttgc
     1861 gtcgaacccg gggatgagga gcacgagatt ctcgacgaca ccgagcaaca atccgcccag
     1921 catggcgccg cgaatgttgc cgataccgcc gaggacggcg gccgcaaagg ccttgacgcc
     1981 aggcgcgaat cccatgttgt acgcaaagct gaagttcaga ccgaacagga agccagccgc
     2041 gccaccgagc aggccgccga tgatgaacgt gcgggagatc gtcttgtcga tgtcgatgcc
     2101 cataagggcg gccgtcggcg catcctcgga gactgcacgg atgctccgcc cgagcttggt
     2161 cagtctgacc agtcgatcca ggaacaccat catcacgatg gccgagatca cgatcacggc
     2221 ctgcagcgct ttgacgttcg cgtggtcgat cttgaacagc gtcgtgttgt ggtcgaagaa
     2281 ggtcgggaac tgattcgttg tctggctgtt gaacagtttg ctggccaggg tcgagagaaa
     2341 gaacgacgcg ccgatcgccc cgatcaagaa ggcaagcttg ggcgcgcctc ttcgtctcag
     2401 tggccgatag gccaccctct ctagagtcca ggcgacgcac gcggcagtca gcgcaccggt
     2461 caccatgccg acggcgatca cgatgggcac ttcccatgtc gcggttcctg agccgtcgcc
     2521 cacgatcagg tgcaccacga tgtagctgcc aaacgccccg gacatgaaca cctcgctgtg
     2581 ggcgaagttg atcagctgca gtaccccgta gaccagtgtg tagcccagcg cgataagggc
     2641 gtacatcgat ccccgggtga gcccgaggat caggtagtcg aagaactggg tcatgcctgg
     2701 atgtccctcc gggagagcat cactccggcg acagtgtgca
//
LOCUS       DATCBJ010000351         7488 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_45194, whole genome shotgun sequence.
ACCESSION   DATCBJ010000351 DATCBJ010000000
VERSION     DATCBJ010000351.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 7488)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7488)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7488
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_45194"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..766
                     /locus_tag="VII50_07005"
     CDS             <1..766
                     /locus_tag="VII50_07005"
                     /inference="COORDINATES: protein motif:HMM:NF014016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sirohydrochlorin chelatase"
                     /protein_id="HEY5050633.1"
                     /translation="CAIAHRGWDMSEPALIAVAHGSADPRAEQVLDALIARVRSERPG
                     VVAVLAHLGFSDPDVSAALESLARQGVREVVVVPLLLTAAYHAKIDLPALLLRAAHDH
                     PQLAMRQAEVLGPHRLLFELVRRRLAEAGVEPGASLVLAAAGTSDPGANAELADVAAS
                     LGATIGFASGWPAITDVVAGLRARGARQVALATYVLAPGELADRLHDAGADVVTEVLG
                     AAPEIVDVVLARYDAALAAGSASGLAATGAASGVIN"
     gene            847..1638
                     /locus_tag="VII50_07010"
     CDS             847..1638
                     /locus_tag="VII50_07010"
                     /inference="COORDINATES: protein motif:HMM:NF014089.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIM24 family protein"
                     /protein_id="HEY5050634.1"
                     /translation="MTLSHKIVGNAMQLAVCQIADGQTIYADAGKFLWKTVNVGIETH
                     IATQSSAAGGGGAGGGLLGMAMNAGKRMLAGESIAFQYFTARGGAGLVSVAGTLPGEM
                     RALELDGTRGWFAEKDSFVAAEGTVTNDIAFSGLRAGFKGGEGFVLQRFGGVGTVLIA
                     GAGNFIDINPAKYGGKIQVHTGCLVAFEDSLKYGVERVGALNAQTIVTGLLGGNGFNL
                     ATLEGDGNAILQSMTIDSLAHAIVQHAHLGHDEKASNPLGSLFGG"
     gene            1647..1997
                     /locus_tag="VII50_07015"
     CDS             1647..1997
                     /locus_tag="VII50_07015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5050635.1"
                     /translation="MGLLDKAKASATAAMAQGQAKVGAFQQGRSEADLYKALGEAFYR
                     EQRQGGDHESTVAALVAVDNALAQAAQAAAAQPAPYQQPAPYQQPAAPGQPYTPPPAS
                     GAAPTAGNFTLDDM"
     gene            2054..3724
                     /gene="ettA"
                     /locus_tag="VII50_07020"
     CDS             2054..3724
                     /gene="ettA"
                     /locus_tag="VII50_07020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019512148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0045900 - negative regulation of
                     translational elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HEY5050636.1"
                     /translation="MPEFIYVFRKARKAYGEKVILDDVTLSFLPGAKIGVVGPNGAGK
                     SSVLRIMAGIEQPSNGEAYMTPSYTVGILDQEPKLDDTKTVLGNVEDGVAATMALLAR
                     YYQVSDELGNPDADSDALLAEMGELQTQLDHLNAWDLDAQLEQAMDALRCPPPDADVS
                     VLSGGERRRVALCKLLLQQPDLLLLDEPTNHLDAESVLWLEQHLEKYPGTVVAVTHDR
                     YFLENVAEWILEIDRGRTYPYEGNYTTYLEAKATRIKVEGAKDQKLRKRLEDELEWVR
                     SNPKARQAKSKARLDRYEEMATEADKYKKLDFDEIQIPPGPRLGNIVVETSGLTKGFD
                     ERLLMEGLSFSLPRNGIVGVIGPNGTGKTTLFKMLIGEERPDAGTIKIGETVRISYVD
                     QNRSGLDPKKSLWEVVSDGLDYIKVGHVEMPSRAYVSAFGFKGPDQQKPTGVLSGGER
                     NRLNLALTLKQGGNLLLLDEPTNDLDVETLASLENALLEFPGCAVITSHDRWFLDRVA
                     THILAYEGDSNWFWFEGNFESYEKNKVERLGVDAARPHRATYRKLTRG"
     gene            3726..4136
                     /locus_tag="VII50_07025"
     CDS             3726..4136
                     /locus_tag="VII50_07025"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024675.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HEY5050637.1"
                     /translation="MRFTFRCPVRWDDMDAFGHVNNVTFLTYMQEARTGMLFAGEAAE
                     HIPDLIKGVVVARHEIDYRRPLEWRPEPIEVDVWTAQVKSSSFVLRYEVRDQLADAVL
                     AEALTVLVPFDLDAARPRRVSSSEREFLSRYLEP"
     gene            complement(4140..5273)
                     /locus_tag="VII50_07030"
     CDS             complement(4140..5273)
                     /locus_tag="VII50_07030"
                     /inference="COORDINATES: protein motif:HMM:NF013180.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HEY5050638.1"
                     /translation="MEPSALTATESLFAGLVPDDPETVIDRLENRLAGQTSDVLRMRK
                     SAQTSQALLDLHTACTRQRTVAGTARQIAAVASAITGSEVVIVSVFQVASQQPLLAVS
                     LSDGVIDEEHIGNLVCDMATQPDMQWLRTESNLRVVDRESASPSTALLLAELHAERVL
                     ITPLFGQGEFVGCIVAKLPRVAPEHDDAAVIDGATALQDHAGDLIHLALVLDELRRRA
                     LHDAVTGLPNLSVYEQRLIRALARRTAAQPGHSEVTAILVQVVQLGRITEIHGATGVQ
                     ELLREIANRLSELAPEHDLGHLGASHFALIAHGDPGAGIDLARTVIEALETPFDVSFG
                     TLRVFCHVGVAVAVDGEGAGSLLPRAEAAARQARDSDEPIVTA"
     gene            complement(5325..6722)
                     /locus_tag="VII50_07035"
     CDS             complement(5325..6722)
                     /locus_tag="VII50_07035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY5050639.1"
                     /translation="MASSAQVREILRARNFRRLYSTRLLSAAADGIFQTALASYVIFS
                     PQNATTPGKAAAAFAALLLPYSIAGPFVGVFLDRWRRQRVLVIANTVKVALVLVVAGL
                     VLANSDGAAFLATAVVALGVNRFFLSALSAALPHVVDPQDLVTANALSTTSGSVMTIV
                     GAAVGAGIRLGTGSSPAAVASIVVLGAVAYGASATVAATIGADLLGPDADERVSSGPR
                     SPWRAVTTMTRELTDAARYVGRRKGAANALGAISAHRFLFGVASLTIVLLSRNYFATQ
                     GNVDGGILGLGASIAALGVGIVVAALITPAATDRFGTTTWITAMLILAGVAIAAFGLP
                     FQRGLLVVGSFLLGVSAQGVKICVDSTVQQQVDDVYRGRVFSVYDMAFNGTYVAAAAV
                     AATVLPKSGKSFTVMALLAGGYLLAAMIFAWSSARTRIPPSAAPTPAASVPGAPRQPV
                     GIETGKSSSGGYRRP"
     gene            complement(6748..7158)
                     /locus_tag="VII50_07040"
     CDS             complement(6748..7158)
                     /locus_tag="VII50_07040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="globin"
                     /protein_id="HEY5050640.1"
                     /translation="MTHTADGSFYDQIGGAEAFGRLVHRFYQGVADDPLLRPLYPDAD
                     LGDAEVRLRMFLEQYWGGPKTYGELRGHPRLRMRHAPFRIGPAERDAWLVHMRAALDE
                     LELDAEHEATLWGYLASAAESLVNSLGGQYETPA"
     gene            7226..>7488
                     /locus_tag="VII50_07045"
     CDS             7226..>7488
                     /locus_tag="VII50_07045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family peroxiredoxin"
                     /protein_id="HEY5050641.1"
                     /translation="MATTRTAHTHWEGPLLAGGKGNVSLDSSGLGSFGVSWAARTEAP
                     GGQTSPEELIAAAHSSCFSMALSNALAKAGHTDITLETKADVT"
BASE COUNT         1329 a         2444 c         2515 g         1200 t
ORIGIN      
        1 atgcgcaatt gctcaccggg ggtgggatat gagcgagccc gcgctgattg ctgtcgcgca
       61 cggaagtgcg gacccacgag ccgagcaggt cctcgacgcg ctgatcgcgc gggtccgctc
      121 ggagcgccct ggtgtggtcg ccgtgctcgc acatctcggt ttctccgatc ccgacgtttc
      181 ggcggcgctt gagtcgctcg cgcggcaggg agtgcgcgaa gtcgtcgtcg tcccgctgct
      241 gttgaccgcc gcttatcacg ccaagatcga ccttcccgct ctgctcttgc gggccgcgca
      301 cgatcatcca caactggcga tgcggcaggc agaagtgctc gggccgcatc ggttgctctt
      361 cgagctggta cgtcggcggc tggccgaggc cggcgtcgag cccggtgcgt ctcttgtgtt
      421 ggccgccgcc ggtacctccg atccaggtgc gaacgccgaa ttggccgacg tcgcggcgag
      481 tcttggtgcc accatcgggt tcgcctccgg ctggccggcg attaccgacg tcgtcgccgg
      541 gctccgcgcg cgtggcgcga ggcaggtcgc gcttgccacc tacgtgctgg cgccgggcga
      601 gctggcggat cgcttgcacg atgcgggcgc cgacgtcgtg accgaggtcc ttggcgccgc
      661 tcccgagatc gtcgacgtgg tcctggctcg atacgacgct gccctcgcgg cgggcagcgc
      721 aagcggcctg gcggcgaccg gcgccgcgtc gggtgtgatc aattgagagc tacccgtttg
      781 ccttgaacat cggtagcgtc gagccctcac caacggcgag atttcttcgg tggagggacc
      841 ggcgctatga ctctttcgca caagatcgtt ggcaacgcga tgcagttggc cgtctgtcag
      901 attgcggatg ggcagaccat ctacgccgac gctggcaaat tcctgtggaa aacagtcaac
      961 gtcggcatcg aaacgcacat cgctacgcag agctcggcag ccggcggcgg cggtgccggg
     1021 ggcgggctgc tcgggatggc gatgaacgcc ggcaagcgga tgctcgcggg cgagtcgatc
     1081 gcgttccagt atttcaccgc gcgcggcggc gccggcctcg tgagtgtcgc gggcacgctg
     1141 cctggtgaga tgcgggcgct tgagctcgac ggcacccgag ggtggttcgc cgaaaaggac
     1201 tcgttcgtgg ccgcagaagg caccgtcacg aacgacatcg ccttcagtgg tctgcgtgcc
     1261 ggcttcaagg gtggcgaggg attcgtcctc cagcgcttcg gcggtgtcgg caccgtgctc
     1321 atcgccgggg ccggcaactt catcgacatc aacccggcga aatacggcgg caagatccag
     1381 gtgcacaccg gctgcctggt cgccttcgag gactccctaa agtacggcgt cgagcgtgtc
     1441 ggtgccctca acgcgcagac catcgtcacc ggtctgctcg gcggtaacgg gttcaacctg
     1501 gcgaccctcg aaggcgacgg caatgcgata ctccagtcga tgaccatcga ctcgctggcc
     1561 cacgcgatcg tgcagcacgc gcacctcggc cacgacgaaa aggccagcaa cccgctcggc
     1621 agtctcttcg gagggtagta acggacatgg gacttctcga caaggccaag gcgtccgcca
     1681 ccgcggcgat ggcgcaaggg caagcaaagg tcggcgcctt ccagcagggt cgctccgaag
     1741 ctgacctgta taaggcgctg ggcgaagcgt tctatcgcga gcagcggcag ggcggcgacc
     1801 acgaatcgac cgttgccgcg ctcgtcgcgg tcgacaacgc gctggcgcag gcagcacagg
     1861 cggcagccgc ccagccggcg ccctaccaac aacccgcgcc gtatcagcag ccggcggcgc
     1921 ccggccagcc ctacacgcca cctccggcaa gtggtgctgc gccgaccgcg gggaacttca
     1981 ctctcgacga catgtagcca cggcagcacg cggtccaggg tgagttgatc gacgtcccgt
     2041 agactcgtcg accgtgcccg aattcattta cgtcttcaga aaagcgcgca aggcgtacgg
     2101 cgagaaggtc atcctcgacg acgtcacctt gtcgttcctg cccggcgcca agatcggtgt
     2161 cgtcgggcct aacggcgcgg gtaagtccag cgtgctgcgg atcatggctg gcattgagca
     2221 gccgagcaac ggcgaggcct acatgacgcc gagctacacc gtcgggatcc tcgaccagga
     2281 gccgaagctc gacgacacca agacggttct cggcaacgtc gaggacggcg tggcggcgac
     2341 gatggccctg ttggcgcggt actaccaggt ctccgatgaa ctgggcaacc cggacgccga
     2401 ttccgacgcg ctgctggcag agatggggga gttgcagacg cagctcgacc acctcaacgc
     2461 ctgggatctt gatgcccaac tcgagcaggc gatggacgcg ttgcgctgcc cgccgcccga
     2521 cgccgacgtc agcgtgttgt ccggcggcga gcggcgtcga gtcgcgttgt gcaagctgct
     2581 cctgcaacag cccgacctct tgctgctcga cgagcccacc aaccacctcg acgccgagag
     2641 cgtgctctgg ctcgagcagc acctcgagaa gtacccgggc accgtcgtcg cggtcactca
     2701 cgacaggtac ttcctggaga acgtggcgga gtggatcctc gaaatcgacc gaggccgtac
     2761 gtatccctac gagggcaact acacgaccta cctcgaggcc aaggccaccc ggatcaaggt
     2821 cgagggcgcc aaggaccaga agctgcgcaa gcgcctcgag gacgagttgg aatgggtgcg
     2881 gtcgaacccg aaggcgcgcc aggcgaagag caaagcacgt cttgatcgct acgaggagat
     2941 ggccaccgag gcggacaagt acaagaagct cgacttcgac gagatccaga tcccgccggg
     3001 gccgcgcctt ggcaacatcg tggtcgagac ctcggggctg acgaagggct tcgacgagag
     3061 gctcctgatg gaaggcctct ccttttcgct gccacgtaac ggaattgttg gcgttatcgg
     3121 cccgaacggc accggtaaga cgacgctgtt caagatgttg atcggtgaag agaggccgga
     3181 cgccggcact atcaagatcg gtgagaccgt gcggatctcc tacgtcgacc agaacagatc
     3241 tggcctcgac ccaaagaaga gcctctggga ggtcgtatcc gacggcctcg actacatcaa
     3301 ggtcggccat gtcgagatgc cgagccgtgc gtacgtgtcg gccttcggct tcaagggtcc
     3361 cgatcagcag aagccaaccg gcgtcctgtc cggtggcgag cgcaaccggc tgaacctggc
     3421 gctgacctta aagcagggcg gcaacctgtt gctgctggac gaaccgacca acgatttgga
     3481 cgtggagacg ctggcgtcgc tcgaaaacgc gctgctcgag ttccccggct gtgcggtgat
     3541 caccagccac gaccgctggt tcctcgaccg ggtcgcgacg cacatcttgg cgtacgaagg
     3601 cgactcgaac tggttctggt tcgagggcaa cttcgagtcc tacgagaaga acaaggtcga
     3661 gcggctcggg gtcgatgcag cccgtccgca ccgggccacc taccgcaagc tcacccgcgg
     3721 ctagcgtgcg attcaccttc cggtgtcccg tccgctggga cgacatggac gcgttcggcc
     3781 acgtcaacaa cgtgacgttc ttgacgtaca tgcaggaagc ccgcacaggc atgctcttcg
     3841 ccggcgaggc agcagagcac atccctgatc tcatcaaggg tgtcgtggtc gctcggcacg
     3901 agatcgacta tcgccggccg ttggaatggc ggcctgaacc gatcgaagtc gatgtctgga
     3961 cggcccaggt gaagtcgtcg tcctttgtgt tgcgctacga ggtccgcgat caactcgctg
     4021 acgccgtgct ggctgaggcg ctcacggtct tggtgccgtt cgaccttgac gccgctcgcc
     4081 ctcgacgagt ctcgtccagc gagcgcgagt ttctgtcccg ctacctggag ccctgagctt
     4141 taggcggtga cgatcggctc gtcgctgtcg cgagcctgcc gtgcggcagc ttccgcccgg
     4201 gggagcagcg agcccgcgcc ttcaccgtcg acagccacgg cgaccccgac gtggcagaag
     4261 acgcgcagcg tgccgaaact gacgtcgaaa ggcgtctcga gcgcctcgat caccgtgcgc
     4321 gcgaggtcga tcccggcgcc cgggtcaccg tgggcgataa gtgcgaagtg gctcgcgcct
     4381 aggtggccga ggtcgtgctc gggtgccagc tcgctgagcc ggttcgcgat ctcgcgcaac
     4441 agttcctgga caccggtcgc gccgtggatc tcggtgatac gaccgagttg gacgacttga
     4501 accaggatcg cggtgacctc cgagtggcct ggctgcgcgg cggttctgcg cgcgagcgcg
     4561 cgaatcaggc gttgctcgta aaccgagagg ttgggaagcc ccgtgacggc gtcgtgcaga
     4621 gcgcggcggc gcaactcgtc gagcacgagg gccaggtgga tcaggtcacc agcgtggtct
     4681 tgcaaggcgg tggcgccgtc gatgacggcg gcgtcatcgt gctcaggtgc gacacggggc
     4741 agcttcgcca cgatgcaacc cacgaactcg ccctgtccga acagcggcgt gatgaggacc
     4801 cgctccgcgt gcagctccgc gagcagcagt gcggtcgacg gactcgccga ttcgcggtcc
     4861 acgacccgca ggttgctttc ggtgcgcagc cattgcatgt cgggctgggt cgccatgtcg
     4921 cacaccaggt tgccgatgtg ctcctcgtcg atgacgccgt cgctcaggct cacggccagc
     4981 agtggttgct ggcttgctac ctggaagacg ctgacgatca cgacctccga gccggtaatg
     5041 gcggaggcga cggcggcgat ctgacgagcg gtgcctgcga cagtgcgctg gcgggtacag
     5101 gcggtgtgca ggtcgaggag agcctggctg gtctgcgcgc tcttgcgcat ccggaggacg
     5161 tcagaggtct ggccggcgag tcggttctcg agccggtcga taaccgtctc aggatcatcg
     5221 ggcaccagcc cggcgaacag cgactcggtg gctgtcagcg cgcttggctc cacagccgtt
     5281 cctccacctt cgagtggcac ccgacggatg cgtatctaag tcgactacgg ccgccggtac
     5341 cccccacttg aggattttcc ggtttcgatc ccgaccggtt gccgcggtgc gccgggaaca
     5401 gaggcagccg gtgtcggagc cgcagaggga ggaatccggg tgcgagcaga cgaccacgcg
     5461 aagatcattg ccgccagcag gtagccgccc gccaaaaggg ccatgaccgt gaaggacttt
     5521 ccggacttcg gcaagacggt tgccgcaacc gcggccgcgg caacgtacgt gccgttgaag
     5581 gccatgtcgt agaccgagaa cactcggccc cggtatacgt cgtcgacctg ttgttgcacc
     5641 gtcgagtcca cgcagatctt gacgccttgt gccgagacac cgagcagaaa ggacccgacg
     5701 acgagcaggc cgcgctggaa cggcagacca aaggccgcaa tggcgacccc ggcaaggatc
     5761 agcatcgcgg tgatccacgt cgtggtgccg aaccggtcgg tcgcggccgg ggtgatgagc
     5821 gcggcgacga cgatgccgac gccgagggcg gcgatcgacg ctccgagacc gaggatgccc
     5881 ccgtcgacgt taccttgagt ggcgaagtag ttgcggctca gcagcacgat cgtcagcgac
     5941 gccaccccga acaggaaccg gtgcgcgcta atcgccccga gggcgtttgc tgctcccttg
     6001 cgtcgaccga cgtagcgtgc cgcgtcagtc agttcgcgcg tcatggtggt gacggcacgc
     6061 cacggcgaac gcgggcccga tgacacccgc tcatcggcgt ccgggccgag caggtcagcg
     6121 ccgatcgtcg cagcgaccgt agccgacgcg ccgtacgcca cggccccgag caccacgatg
     6181 gacgcgaccg cggctgggct cgaaccggtg cccaaccgga tgccggcacc gaccgccgcg
     6241 ccgacgatcg tcatcaccga tcccgacgtt gtcgacagcg cgttagcagt gaccaggtct
     6301 tgcgggtcga ccacgtgggg caacgcggcg gacagcgcgg agaggaagaa ccggttgact
     6361 ccaagtgcca ccaccgcagt cgccaagaat gcagcgccgt ctgagttggc gagtacgagt
     6421 ccggcgacga ctaggaccag tgccactttg acggtgttgg cgatcacgag cactcgttgg
     6481 cgacgccagc ggtcgaggaa gacaccgacg aagggcccgg cgatcgaata cggcagcagc
     6541 agcgcagcga aggcggcggc tgccttaccg ggcgtcgttg cgttttgcgg ggagaagatg
     6601 acgtagcttg ccagcgcggt ctggaagatg ccgtcggccg ccgcagacaa cagccgagtg
     6661 ctgtagagcc tgcgaaagtt ccgcgcgcgc aggatctcgc gaacctgcgc cgaactcgcc
     6721 acacccgaac cttagtcgcg gctcgcgtca ggcgggggtt tcgtactgcc cgcccaggct
     6781 gttcacgagg ctctcggctg cgctcgccag gtaaccccac aacgtcgctt cgtgctcggc
     6841 gtccaactcc aattcgtcga gcgccgcgcg catgtgtacg agccaggcgt cgcgctctgc
     6901 tggcccgatc cgaaacggag cgtgcctcat ccgcagcctc gggtgcccgc gcagttcgcc
     6961 gtaagttttc ggaccgcccc agtactgctc gagaaacatc cgcagccgca cctcggcgtc
     7021 cccgagatcg gcatcgggat agaggggacg caacagaggg tcgtcggcga cgccctgata
     7081 gaaacgatgc acgaggcgac cgaatgcctc ggcgccaccg atctgatcgt agaaactccc
     7141 gtcagccgta tgcgtcaccg caccattctc gcaaagacac gggcgcaagg cgccaacaca
     7201 ccaagcgcaa ggaaggactg cagccgtggc taccacccgc accgctcaca cgcactggga
     7261 gggcccgctc ctcgctggcg gcaagggcaa cgtcagtctc gactcgtcgg gtctgggcag
     7321 tttcggcgtc agctgggctg cgcgtaccga ggcgcccggg ggccagacca gccccgagga
     7381 actcatcgct gctgctcact cgtcgtgctt ctccatggcg ctgtcgaacg cgctggcgaa
     7441 agccggccac accgacatca cgctcgagac caaggcggac gtgacgtt
//
LOCUS       DATCBJ010000352         2617 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_228622, whole genome shotgun sequence.
ACCESSION   DATCBJ010000352 DATCBJ010000000
VERSION     DATCBJ010000352.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2617)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2617)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2617
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_228622"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..969
                     /locus_tag="VII50_07050"
     CDS             <1..969
                     /locus_tag="VII50_07050"
                     /inference="COORDINATES: protein motif:HMM:NF016047.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding repeat-containing protein"
                     /protein_id="HEY5050642.1"
                     /translation="GGPAAAADPTATAISGSDRYETAAQVAQTFFHTPDVAGVATGTN
                     FPDALAAGPDLASKDAPLLLVPPAGVLPPGTTAQLLDYAQTLRSALVFGGTTSVADSV
                     GAQVGTLANLSATATIESTSSAYTGQFGILSTHLQTGSGAVNTTQVFDGSAGTSTFYT
                     EGSGSKVIPSTPTRADFTAFPTNDPAALESAVNTSFAAVYASEGYTSTNPNAQFLVNA
                     EQVLLNPLASPPLRLAVYVALANLPGTGAKSGAKDANGHAGIMIFGTVSGNVTLDGAQ
                     LGYIFDPLTGNLLEDDIVTTAGTAIASSTVTTFTTASTAPADPYAN"
     gene            969..>2617
                     /locus_tag="VII50_07055"
     CDS             969..>2617
                     /locus_tag="VII50_07055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HEY5050643.1"
                     /translation="MTLRIVIGAVATLVALGLTGRRLWWLYRLIRSGQPATDRAGRRS
                     AAGQSTTVKGPRAEVTEVIGQRKLLAWTIPGLAHAVTFWAFLVLGLTIVEAYGALFKA
                     NFGIGDWAGLGFIEDLFATFVLVALVVFTKIRFLNRASRRGRASRFYGSHTGAAWLIL
                     GMIFTVIATLLLYRGAQMNTGNFPYRPYRWSFASWLVSRALHPLGAANHGVETFFVLA
                     QLIVVLSFLVLVTYSKHLHIVFAPLNIYFARKPDALGPLLPMYSGDHQIDFENPLDDD
                     NFGRGKVEDFTWKGLLDLSTCTECGRCQSACPAWNTGKVLNPKLIITELRDHMFEKAS
                     TLLSGVEAAAAMRPLVGTLEAGGVIDPEALWACTSCGACVQECPVDIEHVDHIMDMRR
                     HQVLVESAFPAEAGGMMRNLENAGNPWGLPASTRLAWTEGLPFDVSVVGRGSQIPDDV
                     EYLFWVGCAGASDDRARASTRAVAELLYTAGVEFAVLGRLETCTGDPARRMGNEFVYQ
                     QLAQQNVATLQSVGARKIVASCPHCFNTIAREYPQIGGDFEV"
BASE COUNT          425 a          854 c          833 g          505 t
ORIGIN      
        1 ggcggccccg cggccgcggc cgacccgacg gcgaccgcga tctcggggtc tgaccgttac
       61 gagacggcgg cgcaggtcgc gcagaccttc ttccacaccc cggatgtcgc aggtgtcgca
      121 accggcacca acttcccaga cgcgctcgcc gcgggacccg atctcgcctc caaggacgcg
      181 ccgctgttgt tggtgccgcc ggccggcgtg ctgccgcccg gaaccaccgc ccagctgctc
      241 gactacgcac agacgctgcg cagcgcgctg gtcttcggcg gcacgacgtc ggtcgccgac
      301 tcggtcggcg cccaggtcgg cacgctggcg aacctgagcg ctaccgcgac gatcgagtcg
      361 acgtcatctg cctacaccgg ccagttcggg atcctcagca cccacttgca gaccggttcg
      421 ggtgcggtca acacaacgca ggtcttcgac ggcagcgcag ggacctccac cttctacacc
      481 gaaggttcgg gatcgaaggt catcccgagc acgccgacaa gagccgattt caccgcgttc
      541 ccgaccaatg acccagcggc actggagtct gcggttaaca cgtcgttcgc ggctgtctat
      601 gcgtcagagg gctacacctc gaccaaccca aacgcgcagt tcctcgtcaa cgccgagcag
      661 gtgctactca acccgctggc atcgccgccg ttgcgcctgg cggtttacgt cgcgctcgcg
      721 aacttgccag gcaccggcgc caagtcggga gccaaggacg cgaacggcca cgccggcatc
      781 atgatcttcg gtaccgtcag cgggaacgtc acccttgacg gtgcccagct cggttacatc
      841 ttcgatccgt tgaccggcaa cttgctggaa gacgacatcg tcaccactgc tggtaccgcg
      901 atcgcgtcca gcaccgtgac gacgtttacg accgcttcca ctgcgcccgc ggacccctac
      961 gcgaactgat gacgctgcgc atcgtcatcg gcgcggttgc caccctcgtg gcactcgggc
     1021 tgacgggtcg tcggttgtgg tggctgtacc ggctgatccg gtcagggcaa ccagcaacag
     1081 atcgggccgg acgccgatca gcggctggtc agtccacgac cgtgaagggg ccgcgggccg
     1141 aggtgaccga ggtcatcggt cagcgcaaac ttctcgcgtg gactattccg ggccttgccc
     1201 acgcggtcac gttttgggca ttcctcgttc tcggactcac catcgttgag gcctacggcg
     1261 cgctgttcaa ggcgaacttc ggcatcggtg actgggcagg gctcggcttc atcgaggatc
     1321 tgttcgcgac gttcgtgctg gttgctcttg tcgtcttcac caagattcgg tttctcaacc
     1381 gtgcatctcg acgtggtcgc gcgtcacgct tctacggttc acacacaggt gcggcgtggc
     1441 tgatcctcgg catgatcttc acggtgatcg cgacgttgct gctctaccgc ggtgcgcaga
     1501 tgaataccgg caacttcccg tatcggccgt accggtggtc gttcgcgtcg tggttggtgt
     1561 cgcgcgcgtt gcacccgctc ggtgcggcga accacggtgt cgagacgttc ttcgtgctcg
     1621 cgcagctgat cgtcgtcctg tcgtttctcg tgctggtgac ctattcgaag cacctgcata
     1681 tcgtgttcgc gccgctgaac atctacttcg cgcgaaagcc cgatgcgctg gggccgctgc
     1741 tgccgatgta ttcgggtgat caccagatcg acttcgaaaa ccctttggac gacgacaatt
     1801 tcggccgcgg caaggtcgag gatttcacct ggaaagggct gctcgatctg tcgacctgta
     1861 ccgagtgcgg tcggtgccag tcggcatgcc ccgcctggaa taccgggaag gtactgaatc
     1921 caaagctcat catcactgag ctgcgcgatc acatgttcga gaaggcgtcc acgctgctgt
     1981 cgggtgtgga ggctgcggcg gcgatgcgtc cgcttgtcgg gacgctcgag gcgggtggcg
     2041 tcattgatcc cgaggcactt tgggcgtgca cgtcgtgcgg cgcctgcgtg caggaatgcc
     2101 ctgtcgacat cgaacacgtc gaccacatca tggacatgcg tcggcatcag gtgctggtcg
     2161 agtcggcatt ccccgccgag gctggcggca tgatgcgcaa cctcgagaac gccggtaacc
     2221 cctgggggtt gcctgcatcg acgcggttgg cgtggacaga gggcctgccg ttcgacgtca
     2281 gcgtcgttgg tcgaggttcg cagattccgg acgacgtcga gtacctgttc tgggtcggtt
     2341 gtgccggtgc atccgatgac cgcgctcgtg catcgacacg tgccgtcgct gagcttctgt
     2401 ataccgccgg agtcgagttt gcggtgctcg gacgactcga gacatgtacc ggagatccgg
     2461 cgcggcggat gggcaacgag ttcgtctacc agcagctggc acagcagaac gtcgcgacgc
     2521 tgcagtcggt cggcgcacgc aagatcgtcg cctcctgccc gcactgcttc aacacgattg
     2581 cgcgtgagta cccgcagatc ggcggcgact tcgaggt
//
LOCUS       DATCBJ010000353         5703 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_73875, whole genome shotgun sequence.
ACCESSION   DATCBJ010000353 DATCBJ010000000
VERSION     DATCBJ010000353.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 5703)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5703)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5703
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_73875"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            573..2414
                     /gene="infB"
                     /locus_tag="VII50_07060"
     CDS             573..2414
                     /gene="infB"
                     /locus_tag="VII50_07060"
                     /inference="COORDINATES: protein motif:HMM:TIGR00487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HEY5050644.1"
                     /translation="MQAPSIGGVQVPRGNGQVLRLPRGSSLSDFADRIGANPASLVQV
                     MFHLGEMVTATQSVNEETLQLLGAELGYDVQVVSPEDEDRELLESFDLEFGEDEGGEG
                     DLTARPPVVTVMGHVDHGKTKLLDAIRNTNVVAREHGGITQHIGAYQVIADSIAGKRA
                     ITFIDTPGHEAFTAMRARGAQVTDIAVLVVAADDGVMPQTIEALNHAQAAGVPIVVAV
                     NKIDKEDADPTKVRGQLTEYGLVAEEYGGDTMFVDVSAKSNLNIDKLLEAILLTADAA
                     LDMRANADQDAQGVAIEAHLDRGRGPVATVLVQRGTLRVGDSVVAGEAFGRVRAMLDE
                     FGETVAEAGPSRPVQVLGFTSVPDAGDNFLVVPEDRVARQIAERRSARERNAQLADSR
                     GRRSLEDILARMEKGEVEELRLILKGDVSGSVEALEDALLNLDVGEDVDLRVIDRGVG
                     AITQNDVNLAVASDAVIIGFNVRPAERVSELIDREGVDVRYYSVIYQAIDEVEAALKG
                     LLKPEFEEARLGTAEIRDVFRSSKFGNIAGCLVRSGTITRNSKARLIRDGVVINDHLT
                     IESLKRFKDDATEVREGYECGIGLGSFNDIKVDDVIETFEMKEKPRA"
     gene            2460..2756
                     /locus_tag="VII50_07065"
     CDS             2460..2756
                     /locus_tag="VII50_07065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF503 domain-containing protein"
                     /protein_id="HEY5050645.1"
                     /translation="MFAGVLEIDLLLGDVHSLKEKRSLIRPVVAEVRRKFEVSVAEVG
                     HLELHRRALVGVAVVAAEAARCVEVLEACERLVAGRPEVEVLSTRQRLFGEEDE"
     gene            2756..3187
                     /gene="rbfA"
                     /locus_tag="VII50_07070"
     CDS             2756..3187
                     /gene="rbfA"
                     /locus_tag="VII50_07070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009080499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HEY5050646.1"
                     /translation="MADHARARKLADRIRVVVAETLERQVKDPRLGFITITDARVTGD
                     LHDATVFYTVLGDDSARAETAAALESARGVLRSEVGKQTQVRFTPTLTFVLDGVLDNV
                     QHIDELLAKAREADAAVHEAATHAVPAGDADPYRVAPGAAE"
     gene            3188..4090
                     /gene="truB"
                     /locus_tag="VII50_07075"
     CDS             3188..4090
                     /gene="truB"
                     /locus_tag="VII50_07075"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HEY5050647.1"
                     /translation="MSKGSAEPPLTGGLVVVDKPAGLTSHDVVARVRRLAGTRRVGHA
                     GTLDPMATGVLLVGIGRATRLLGHLALHDKDYVATMRLGVMTSTDDAEGERLAHTAAD
                     HLASADVVAAMQDFTGEIGQRPPAVSAIKVDGVRAYSRARSGEDVVLAARRVTVSRFE
                     ATQFRPDAELGVLDVEVEVTCSSGTYIRALARDVGTALGVGGHLSALRRTRVGAFDLS
                     VARTLTELETDFTLTPLDDVAASAFARRDLDAEQALALSHGAKLSASGKGDEPVAAFG
                     PDGALVALLEDRGDIARALAVFVG"
     gene            4177..5118
                     /locus_tag="VII50_07080"
     CDS             4177..5118
                     /locus_tag="VII50_07080"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: protein motif:HMM:NF004160.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HEY5050648.1"
                     /translation="MQVWRDGTGGVPADVGRTVVTIGVFDGVHRGHQRIVYRALARAA
                     ELRLPCVAVTFEPLPEFVVRPLAKPALLTTLDRRLELLGELGLDGTYVIDFTTEFSHH
                     SPQGFVQSVLVERLRAVDIVVGANFRFGRRAAGDVATLAELGGTAGFMVDPIQLAGPI
                     SEPDSEASSKVYSSSWIRERIAVGDVEGAAEALGRPHLLEGTVIHGDHRGRELGYPTA
                     NLEVAPDLAIPADGVYAGWLDAMPAAISIGTNPTFGGTSRRVEGYAIDEVGLDLYGSV
                     VALDFAARIRPMVTFDSVEALVAQMAADVEECRRRLM"
     gene            5280..5549
                     /gene="rpsO"
                     /locus_tag="VII50_07085"
     CDS             5280..5549
                     /gene="rpsO"
                     /locus_tag="VII50_07085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011874883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HEY5050649.1"
                     /translation="MALDTATKNSILAEYGTSEGDTGSPEAQVAMLSKRIADLTEHLK
                     VHKHDHHSRRGLLLLVGRRRRLLKYLANKDITRYRTLIERLGLRR"
BASE COUNT          873 a         1846 c         1998 g          986 t
ORIGIN      
        1 ctgccgtccc gcgggcgacc tcaggcccgc cgcggatggg caacaatccc ttcacctccc
       61 aggcgtcaac cgcgccgcgt ccgccgctcg cgccaggtgc cgttccgggc gcgccgcgac
      121 ccgttggccc aggtgccggc gcgccgccgc gacccgctgg tatgccgccg cgccccggcg
      181 gcgctggcgg tatcggcgcc aatcgaccgt cacccgctgg tatgccgccg cgccccgcac
      241 ctggtgggcc gggtggttac ggcgctcgtg gccccgcaac cggtggtccc ggagctggcc
      301 gtccggccgg tcccggtggt gcccgcggtg ggttcacctc ggcacgtcct ggtggtcccg
      361 gcgttggtcg tggcggtccc ggcggcggcc ctggcggccc cggtggtccg ggcgctggtc
      421 ctgggggtcc ccccggagct ggcggtggtc gtcccggtcc tggtggtcgc ggtcgtggcg
      481 gcggcacagc cggtgcgttc ggccgtggac ccggcggtcg gcccacccgt ggtcgcaagt
      541 cgaagaagca gcggcgccaa gagttcgaca acatgcaggc cccgtcgatc ggcggcgtgc
      601 aggttccgcg tggcaatggt caggtgctgc ggttgccgcg tggctcgtcg ctgtctgact
      661 tcgccgaccg catcggtgcc aacccggcgt cgctcgtcca ggtgatgttc cacctcggcg
      721 agatggtgac cgcgacccag tcggtcaacg aagagacgtt gcaacttctc ggtgccgagc
      781 tcggctatga cgttcaagtc gtcagccccg aggacgagga ccgcgagttg ctggagagct
      841 tcgacctcga gtttggcgag gacgaaggcg gcgagggcga cctcaccgca cgcccgccgg
      901 tggtcaccgt catgggtcac gtcgaccatg gcaagaccaa gctgctcgac gcgatccgta
      961 ataccaacgt ggtcgcccgc gagcacggcg gcatcactca gcacatcggt gcctaccagg
     1021 tcatcgcgga ctcgatcgcc ggtaagcggg cgatcacctt catcgacact ccgggtcacg
     1081 aggcgttcac cgcgatgcgt gcccgtggtg cgcaggtgac cgacatcgcg gtcctggtgg
     1141 tcgcggccga tgacggcgtc atgccgcaaa ccatcgaggc gcttaaccac gcccaggcag
     1201 ccggcgtccc gatcgtcgtg gccgtcaaca agatcgacaa ggaagacgcc gacccgacga
     1261 aggtgcgcgg gcagctgacc gaatatgggt tggtcgccga ggaatacggc ggcgacacga
     1321 tgttcgtcga cgtgtcggcg aagtcgaacc tcaacatcga caagctgctc gaggcgatcc
     1381 tgctgaccgc agacgcggcg ctcgacatgc gggcaaacgc cgaccaggac gcgcaagggg
     1441 tcgccatcga ggcacacctc gaccgtggcc gtggtccggt cgcgactgtc ctggtgcaac
     1501 gcggaacgct gcgtgtcggc gactcggtcg ttgccggtga ggcgttcggc cgggtgcgcg
     1561 cgatgctcga cgagtttggc gagaccgtcg ccgaggctgg gccctcgcgc ccggttcagg
     1621 tgctcgggtt cacgagcgtt ccggacgccg gcgacaactt cctcgtcgtc cccgaggacc
     1681 gggttgcgcg gcagatcgcc gagcggcgtt cggcccgcga gcgcaacgcc caactcgccg
     1741 acagtcgtgg tcgacgcagt ctcgaagaca tcctcgcccg gatggagaag ggcgaagtcg
     1801 aagaactccg cctcatcctc aagggcgacg tctccggttc ggttgaggct ctcgaggacg
     1861 cgttgctcaa cctcgatgtc ggtgaggacg tcgacctgcg ggtcattgac cgtggggtcg
     1921 gtgccatcac gcaaaacgac gtcaaccttg ccgttgcctc ggacgcggtc atcatcggct
     1981 tcaacgtccg gccggcggag cgggtcagcg aactcatcga tcgcgaaggc gtcgacgtcc
     2041 ggtactactc ggtcatctac caggcgatcg acgaggtcga ggcggccctc aagggcttgc
     2101 tcaagcccga gttcgaagag gcgcgacttg gtaccgccga gattcgcgac gtcttccgct
     2161 cgagcaagtt cggcaacatc gccggctgcc tcgtgcgctc cggaacgatc acccgaaact
     2221 ccaaggctcg cttgatccgc gacggtgtgg tgatcaacga ccacctcacg atcgagtcgc
     2281 ttaagcgatt caaggacgac gccactgagg tccgcgaggg gtacgagtgc ggcattgggc
     2341 tcgggtcgtt caatgacatc aaggtggatg acgtgatcga gacgttcgaa atgaaggaga
     2401 agcctcgggc ttaggccgtt cccgccgttc gaacccgatc actgaagagg cggaagagca
     2461 tgtttgctgg ggtgcttgag attgatctgc ttcttgggga tgtgcattcc cttaaggaga
     2521 agcgcagtct catccggccg gtagtcgctg aggttcggcg gaagtttgag gtttcggttg
     2581 ctgaggtcgg gcatcttgag ttgcacaggc gcgctttggt tggagttgct gtggttgcgg
     2641 cggaggccgc gcgttgtgtt gaggtgttgg aggcttgcga gcgactcgtt gctggccggc
     2701 ccgaggtcga ggtgctgtcg actcgacagc ggctctttgg cgaagaggat gagtgatggc
     2761 tgaccacgcg cgcgcacgaa agctggctga ccgcattcgg gttgttgtcg ccgagacact
     2821 cgagcggcag gtgaaggacc cgcgcctggg attcatcacg atcacggatg ctcgggtcac
     2881 tggtgatttg cacgatgcga ccgtcttcta caccgtgctc ggcgacgaca gcgctcgcgc
     2941 cgaaaccgct gcggcgctcg agagcgctcg cggcgtgttg cgttctgagg tcggcaagca
     3001 gacgcaggtt aggttcacac cgaccctcac gttcgtgctc gacggagtgc tcgacaacgt
     3061 tcaacacatt gacgagctgc tcgcgaaagc gcgcgaggct gacgcggcag tgcacgaggc
     3121 cgcgacgcac gctgttccgg ccggggacgc cgatccgtac cgcgtcgcgc cgggtgcggc
     3181 ggagtaagtg tcgaaaggct cggccgagcc gccgttgacc ggcggacttg tggttgtcga
     3241 caagccggcg ggactcacct cgcacgacgt cgtcgcccgc gtgcgccggc tcgctggcac
     3301 tcgccgcgtc gggcatgccg gcacgctcga cccgatggct actggggtgt tgctggtcgg
     3361 catcgggcgg gcgacccggt tgctgggcca tctcgccctg cacgacaagg actacgtggc
     3421 gacaatgcgt cttggggtca tgacctccac cgatgacgcc gagggggagc ggctcgctca
     3481 caccgcggcc gatcacctgg cgtctgctga tgttgtcgcc gcgatgcagg acttcaccgg
     3541 cgagatcggg caacgccctc ccgcggtcag cgcgatcaag gtcgacgggg tgcgtgcgta
     3601 ctcgcgtgcg cggtccggcg aggacgttgt gctcgccgcg cggcgggtca ccgtgagcag
     3661 gttcgaggcg acccagttcc gacctgacgc cgagctcggc gtgctcgacg tcgaggttga
     3721 agtcacctgc tcctcgggga cctacatccg cgccctggcc cgcgatgtcg gtaccgctct
     3781 tggcgtcggc gggcatctga gcgcccttcg gcgaacgcgg gtcggtgcct tcgatctgag
     3841 cgttgcccga acgctgaccg agctcgaaac tgatttcacg ctgacgcctc tcgacgacgt
     3901 tgcggcgagt gcgttcgcgc ggcgtgacct cgacgccgag caggcgcttg cgctgtcgca
     3961 cggggccaag ctctcggcgt ccggcaaagg cgacgaaccg gttgccgcgt ttgggccgga
     4021 cggcgcactg gttgcgctgt tggaggaccg tggcgacatt gcgcgcgcac tcgccgtctt
     4081 cgttggctga cagccatggg accctggcgc cgtaggcgtt aagtgtcggg cacctcgcgg
     4141 gcgcgagtcg ggcacataag ggaggcgcgg tcttcggtgc aggtctggcg agacggcacg
     4201 ggtggcgtgc ctgccgatgt gggccgcact gtcgtcacca tcggcgtctt cgacggtgtg
     4261 caccgcgggc accagcgcat cgtctaccgc gcgttggccc gggcggccga gctccgcctg
     4321 ccttgcgtcg ctgtcacgtt cgagccgttg ccggagttcg tcgtccgacc gctcgcgaaa
     4381 ccggcgctgc taacaaccct tgaccgccgg ctcgagctgc tcggcgaact cggcctcgac
     4441 ggcacgtacg tcatcgactt caccactgag ttctcgcacc actcgccgca ggggtttgtt
     4501 cagtcggttc tggtcgagcg gttgcgggcc gtcgacatcg tggtcggtgc gaacttccga
     4561 ttcggacgcc gagcagcggg cgatgtggcg acgctcgccg aactgggggg tacggctggc
     4621 ttcatggtcg acccgattca gctcgccggg ccaatctccg aaccagatag cgaggcatcg
     4681 tcgaaggtgt actcgtcgag ctggattcgc gagcggatcg cggtcggcga cgtcgagggc
     4741 gcggccgagg cgctcggtcg cccgcacctg ctcgagggga ccgtcatcca cggcgaccat
     4801 cgcggacgcg agcttggtta cccgaccgcc aacctcgagg ttgcgcccga ccttgccatc
     4861 ccggcggacg gggtatacgc cggctggctg gacgccatgc cggcggcgat ctccatcggg
     4921 accaacccca cgtttggggg taccagccgg cgagtcgagg gctacgccat tgacgaggtg
     4981 gggctcgatc tgtacgggtc cgtcgtggcg ctggacttcg ccgcccggat acgaccgatg
     5041 gtgaccttcg acagcgtcga ggccctggtg gcgcagatgg cggcggatgt ggaggagtgc
     5101 cgaaggcgcc tgatgtagtg ccgaaggcgc ctcacgtagt gccgcaggcg gctggcagat
     5161 tcgtaccgct gagcgccccg gtggtaacgt ggctcatatg tcggtcgcgt accggcgtgt
     5221 gttttcctgc cctcgtgact cggaccgagg gcgcacacaa agcactgaaa aggagtcccg
     5281 tggcgctcga caccgcgaca aagaactcga ttctggccga atacggcacc agcgagggcg
     5341 acactggatc tcccgaggcg caggtggcga tgctttccaa gcggatcgcc gacctgaccg
     5401 aacacctcaa ggtgcacaag cacgaccacc acagccgccg cggccttttg ctgctcgtcg
     5461 gccggcgtcg tcgcttgctg aagtatctcg ccaacaagga catcacccgc taccgcacgc
     5521 tcatcgagcg tctgggtctg cgtcgatagc gggactgaag cgatcgggcc tccgctggcc
     5581 ggtcgcttct tcgtagtacc gcgccagctg gaaccccagc tgcgcgcacg ctctcaccga
     5641 tatgggagcc cgaacgagcg ccgcctggcc ggtcctcggt agtggtttcc gggcgtgagc
     5701 cgc
//
LOCUS       DATCBJ010000354         3227 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_80092, whole genome shotgun sequence.
ACCESSION   DATCBJ010000354 DATCBJ010000000
VERSION     DATCBJ010000354.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 3227)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3227)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3227
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_80092"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1899)
                     /gene="uvrB"
                     /locus_tag="VII50_07090"
     CDS             complement(<1..1899)
                     /gene="uvrB"
                     /locus_tag="VII50_07090"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HEY5050650.1"
                     /translation="MAPLQVVSDFTPSGDQPTAIADLEQRIKRGDRDAVLLGATGTGK
                     SATTAWLVERLQRPTLVMAPNKTLAAQLANELREMLPNNAVEYFVSYYDYYQPEAYIP
                     QTDTYIEKDSSINDEVERLRHSATNSLLTRRDVVVVASVSCIYGLGTPQEYVDRMVRL
                     KVGQEIERDKLLRKLVEIQYTRNDVAFTRGTFRVRGDTIEIIPQYEELAVRIEMFGDT
                     IERLSTLHPLTGELVTEDQELYVFPASHYVAGPERMERAIAGIEAELEERLAELEKQG
                     KLLESQRLRMRTTYDLEMMRQVGSCSGIENYSRHIDGREAGTAPNTLLDYFPEDFLLV
                     IDESHVTVPQIGGMYEGDMSRKRNLVEHGFRLPSAMDNRPLRWEEFVDRIGQTVYLSA
                     TPGPYELGRVQGDVVEQVIRPTGLVDPEIVVKPSKGQIDDLVHEIRLRAERNERVLVT
                     TLTKKMSEDLTDYLLELGIRVRYLHSEVDTLRRVELLRELRLGEFDVLVGINLLREGL
                     DLPEVSLVSILDADKEGFLRSGTSLIQTIGRAARNVSGQVHMYADTVTPSMAHAIDET
                     NRRRAKQVAYNVERGIDPQPLRKKIADILDSIVREDVDTQALIGGGGRQMSRGKAPVP
                     GLSSRGAGA"
     gene            complement(1999..2343)
                     /locus_tag="VII50_07095"
     CDS             complement(1999..2343)
                     /locus_tag="VII50_07095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5050651.1"
                     /translation="MNRSGRQLLAVALLVTLAGCTRHGSQLGTVTGVARMYGGPLVHG
                     KMADDGNPGPGITLTATHNGRPVASVVTGADGSYRFTLAAGSYVVTGCAGITVVVTAG
                     HIDQQDIGCPIP"
     gene            complement(2660..>3227)
                     /gene="coaE"
                     /locus_tag="VII50_07100"
     CDS             complement(2660..>3227)
                     /gene="coaE"
                     /locus_tag="VII50_07100"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: protein motif:HMM:TIGR00152.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HEY5050652.1"
                     /translation="GKSAVLDELVALGAVGIDADAVARDVVLPGTPGFDAVAAAFGPG
                     VVAADGRLDRRRLAAVVFADAGERARLEAILHPLVRDETRRRSAAADRGAVVVNAVPL
                     LVETGLAGEYDVVVAVQASLALRLDRVLESRAMSDGDARARIAVQASDEERAAVAWWV
                     IVNEGTLEELRAEVARLWTALLARRDAQ"
BASE COUNT          553 a         1095 c         1011 g          568 t
ORIGIN      
        1 cgccccggcg ccacgggacg acagccccgg caccggcgcc ttgcctcggc tcatctgccg
       61 gccaccaccg ccgatgaggg cctgcgtatc aacgtcctcg cgaacgatcg agtcgaggat
      121 gtcggcaatc ttcttgcgca acggctgcgg gtcgatcccc cgttcgacgt tgtaggcgac
      181 ctgcttggcg cgacgccggt tggtctcgtc gattgcatgg gccatcgatg gcgtgacggt
      241 gtcggcgtac atgtgcacct gacccgacac gttgcgcgcg gctcggccga tggtctggat
      301 cagcgaggtg cccgagcgca ggaacccttc cttgtcggcg tcgaggatcg acaccagcga
      361 cacttcgggt aggtcgagac cctcccgcag gaggttgatg ccgacgagca cgtcgaactc
      421 gccgagtcgg agctcacgca gcaactcgac gcgacgcaag gtgtcgacct cgctgtgcaa
      481 gtagcgcacc cgaatgccga gctcaagcag gtagtcagtg aggtcctcgg acatcttctt
      541 cgtcaacgtc gtgacgagta cccgctcgtt gcgctcggcg cgcaagcgga tctcgtgcac
      601 gaggtcgtcg atctgaccct tgctcggctt caccacgatc tcggggtcaa cgagaccggt
      661 cggcctgatc acctgctcga cgacatcgcc ctgcacccgc cccaactcgt acggcccagg
      721 ggtcgccgag agatagaccg tctgaccgat gcggtcgacg aattcctccc aacggagcgg
      781 ccgattgtcc atcgcgctcg gcagtcgaaa accgtgctcg acgaggttgc gtttgcgcga
      841 catgtcgccc tcgtacatgc ccccgatctg gggcaccgtg acgtgtgact cgtcgatgac
      901 aagcaggaag tcctcgggga agtagtcaag caacgtgttc ggcgcggtac ccgcttcgcg
      961 accgtcgatg tgccggctgt agttctcgat acccgagcaa ctgccgacct gccgcatcat
     1021 ctcgaggtcg taagtcgtgc gcatgcgcag ccgttgcgac tcgagcaact tgccttgctt
     1081 ttcgagctcg gcaaggcgtt cttcgagctc tgcctcgata ccggcgatcg ctcgctccat
     1141 gcgctcggga cccgcgacat agtgcgaggc cggaaacacg tacagctctt ggtcttcggt
     1201 gaccagctcg cccgtcaatg gatgcaacgt cgacaaccgc tcgatcgtgt cgccgaacat
     1261 ctcgatgcgc accgcaagct cttcgtactg agggatgatc tcgatcgtgt cgccgcgcac
     1321 ccggaacgtg ccgcgggtga atgccacatc gttgcgggtg tattgaatct cgaccagctt
     1381 gcggagcagc ttgtcgcgct cgatctcttg cccgaccttg agcctgacca tgcggtcgac
     1441 gtactcctgc ggggtgccga ggccgtaaat gcacgaaacg ctcgccacca caacgacgtc
     1501 acgccgggtc agcaggctat tcgtggccga gtgccgcaac cgctcgacct cgtcgttgat
     1561 cgacgagtct ttctcgatgt aggtatcggt ctgcgggatg taagcctcgg gctggtagta
     1621 gtcgtagtag ctcacgaagt actcgacggc attgttcggg agcatctcgc gcaactcgtt
     1681 ggccagctgg gcggccaggg tcttgttcgg agccatcacc aaggtagggc gctgaagccg
     1741 ctcgacgagc caggcagtgg tggccgactt accggtaccg gtggcgccga ggagtaccgc
     1801 atcgcggtcg ccacgcttga tccgctgctc gagatcggcg atcgcggtcg gctggtcgcc
     1861 ggacggggtg aagtcgctga caacctgcag cggtgccacg ctgcgcacaa gatcggtgac
     1921 tggtctcacg cagaacaccg tacgtggtcc gtcggacagt ttcctccgcg cggaaaccac
     1981 caggtcctcc aggagccttc acgggatcgg gcaaccgatg tcctgctggt cgatgtgccc
     2041 ggcggtgacg acgaccgtga tgcccgcgca accggtgacg acgtagctgc cggcagccaa
     2101 ggtgaagcga tagctcccgt cagctccggt caccaccgat gccaccggcc ggccgttgtg
     2161 agtcgccgtc agcgtgatgc cgggaccggg gttaccgtcg tcggccatct tcccgtgtac
     2221 gagcggtccg ccgtacatcc gcgccacccc agtgacggtt cccagctgcg agccgtgacg
     2281 agtgcaacca gcaagagtca ccagaagagc cacggccagc aactgccgtc cgctccggtt
     2341 catggccctg accttcatct gggtctgacg cagctgcgca ccttgcggtt gcgtagcccc
     2401 caagaggcat agccgaggtg cggtcgggac gggtgtgacg ggcgagcata gctggtgcca
     2461 tagtggccaa agattctcgc aaaattcgct tcccagtaag cggtttctcc cctaacgttt
     2521 cgtctcgcaa gaagtgatcc gccgctctcg ggcaaccggg aaggtgtcgc agctggcttc
     2581 ggtcggcggt gacgggcggg gagccacttg caatgtgacc ccctgggctt cggctcgggg
     2641 ggtcacgtaa tttccacccc tattgagcgt cgcggcgggc gagcagcgcc gtccaaagtc
     2701 gcgcgacctc ggctcgcaac tcctccaggg tcccctcgtt gacgatgacc caccacgcga
     2761 ctgcggcgcg ctcctcatca gaggcctgga cggcgattcg cgcgcgcgca tcgccgtccg
     2821 acatcgcccg agactccagt acccggtcca gccgcagcgc cagcgacgcc tgcaccgcca
     2881 cgacgacgtc gtactccccc gctaagcccg tctcgaccag caacgggacc gcgttgacga
     2941 caaccgcgcc acgatccgcc gcggcactcc tgcgccgcgt ttcgtcccgc accagcgggt
     3001 ggagaatggc ctcaagacgg gcccgttcac cggcgtccgc gaacaccacg gctgcgaggc
     3061 gacgccgatc gagcctgcca tcggccgcga cgacgcctgg gccgaacgcc gccgccaccg
     3121 catcgaatcc cggcgtgccc ggcagcacga catcgcgcgc gactgcatcg gcgtcgatac
     3181 caaccgcgcc tagggcaacc agttcgtcga ggaccgcact cttgccc
//
LOCUS       DATCBJ010000355         2866 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_119943, whole genome shotgun sequence.
ACCESSION   DATCBJ010000355 DATCBJ010000000
VERSION     DATCBJ010000355.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2866)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2866)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2866
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_119943"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..572
                     /locus_tag="VII50_07105"
     CDS             <1..572
                     /locus_tag="VII50_07105"
                     /inference="COORDINATES: protein motif:HMM:NF033748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="class F sortase"
                     /protein_id="HEY5050653.1"
                     /translation="APVSESPMDSILPSAPDVPSAVATAAELALLPIAKAMSKSTPVR
                     VQVPSIGVDSSLMDLGLLADGSLQVPPAAFPAGWYTGAPTPGQLGPAIIVGHIDWAGV
                     DGVFYRLHSLLPGAEIDVTRADGSTAVFRVLSVHEYAKDAFPTKLVYGNTAYPALRLI
                     TCGGAFNAKAHSYEDDIVAYAELVGPAAT"
     gene            653..1741
                     /locus_tag="VII50_07110"
     CDS             653..1741
                     /locus_tag="VII50_07110"
                     /inference="COORDINATES: protein motif:HMM:NF025200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4192 domain-containing protein"
                     /protein_id="HEY5050654.1"
                     /translation="MTTDTQVRIKLRSPADIIDAVPYLVGFEPHESLVVLSLRGPRSR
                     LGITARIDLPGARSATACARNFVGYLKRDGAARAIIVLYPPSDGISYPSVKPLAAALT
                     KHLTRARIEVSEVLCVCDGRWWSLQCADAECCPPSGTLIARDATSEIAAAMTVEGRVV
                     LGSREELAHMLDPVGGVGAAAMACAIPRVREEFGALVAAGDRREVVAESLDLFRAAVQ
                     TRIDPEARSDLEARLAPGARSDPELTVDEAARLIAALDYWGVRDEVLTWFDGDRGDAT
                     RSLLLELARRSVLPGEAAALTVLAWVCYLQGDGVLAGIAIDRARTADPQYSLAELLDQ
                     VLGGAVNPSLFHETIASAEFRRAREAGS"
     gene            2062..2649
                     /locus_tag="VII50_07115"
     CDS             2062..2649
                     /locus_tag="VII50_07115"
                     /inference="COORDINATES: protein motif:HMM:NF015596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5050655.1"
                     /translation="MRSSPIIKATVFLGMAALLVAGCASKKSSSSTPTPAAGASTAAA
                     AAPASAAPAAGGSGAAAAPAGPATVAAQMGALGNYLTDGSGKSLYLYTPDTSSTSTCY
                     GQCIAFWPPLLTTGAPKAGTGATASMLGTSPRTDGTTQVTYNGHPLYSFKGDKAAGDT
                     SGQGKAGTWFLVTPAGTQIAAAASSAAAPPASGKY"
     gene            2686..>2866
                     /locus_tag="VII50_07120"
     CDS             2686..>2866
                     /locus_tag="VII50_07120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5050656.1"
                     /translation="MITSKSASGRIERDAVRSRPGKQRHTTEQRPQPSSTHVRESQDE
                     ERDAKNNTGDQGDGLP"
BASE COUNT          474 a          948 c          954 g          490 t
ORIGIN      
        1 ctgcaccggt atcggagtcg ccaatggact cgatcctgcc gtccgcaccc gacgtcccat
       61 cggctgttgc taccgcggct gagctcgcgc tgctgccgat cgcgaaggcg atgagcaaat
      121 cgacgccggt gcgagtgcag gttccgtcga taggcgtgga cagttcgctg atggacctgg
      181 gactgctggc tgatggatcg ctccaggtgc cgcccgctgc gttccccgcg ggttggtaca
      241 cgggtgctcc aaccccggga cagctcggac cggccatcat cgtcggacac attgactggg
      301 caggcgtcga cggcgtgttc tatcggctgc actcactgct gccaggcgcc gaaatcgacg
      361 tcacgcgtgc tgacggcagc actgctgttt tccgcgtact tagcgtgcat gagtacgcca
      421 aagatgcatt cccgacaaag ctcgtctacg gcaacaccgc ctaccccgcg ctgcgattaa
      481 tcacctgtgg tggcgcgttt aacgccaagg cgcatagcta cgaagacgac atcgtcgcgt
      541 acgcggaact cgtggggccc gcagctacgt aggctctgtg cgtaacctcg cggtatccac
      601 aacttcgcgt tctgctgcgt caagcggtgt gtcggcgccc agtctttgtc gcatgacaac
      661 agacacgcag gtccggatca agcttcgttc gccggccgac atcatcgacg cagtgcccta
      721 tctggtggga ttcgagccgc acgagtcgct cgtcgtgctg tcgttgcggg gcccgcgcag
      781 tcggctcgga ataaccgcgc gcatcgatct gccgggagca agatctgcga cggcgtgtgc
      841 gcgcaacttc gtcggctacc tcaagcgcga cggcgcggcg cgggcgatca tcgtgctcta
      901 cccgccgtcc gatggcatct cttatccgtc ggtcaaaccg ctcgctgctg cgctgaccaa
      961 acacctgacg cgcgctcgca tcgaggtcag cgaggtgttg tgcgtgtgcg atggccgatg
     1021 gtggtcactg caatgcgcgg acgccgagtg ctgcccgccg tccggaacgt tgatcgctcg
     1081 tgacgcgacc tctgaaatcg ccgccgcgat gactgtcgaa ggccgcgtcg tgttggggtc
     1141 gcgcgaagaa ctcgcgcaca tgctcgaccc ggtaggcggc gtcggagcgg cggcgatggc
     1201 gtgtgcgatt ccgcgcgtgc gtgaggagtt cggcgcgctg gtagctgccg gtgatcgccg
     1261 tgaggtagtg gccgaatcac ttgatctctt tcgcgcggcc gttcaaacgc ggattgatcc
     1321 cgaggcgcgg tcggatctcg aggcgcggct ggctcctggg gcgcggagtg atccggagct
     1381 cacagttgat gaggctgcac gcctgatcgc ggccctcgac tattggggcg tgcgcgacga
     1441 agtcctgacc tggttcgacg gagaccgagg tgacgcgacc cgctcccttc tgctggagct
     1501 ggcccggcgc tcggtgctgc caggtgaggc ggcggcgctc acggtcctcg cgtgggtctg
     1561 ctacctccag ggcgacggcg tgctcgctgg gatcgcgatc gatcgcgcgc gcacggccga
     1621 tcctcagtac agcctcgcgg aactgctcga ccaggtgctg ggcggcgccg tcaatccgtc
     1681 gctgttccac gagaccatcg ccagcgccga atttcggcgt gcacgggagg ctgggtcatg
     1741 acgcctcgta tggggtgttt ccaccggtct tgaaatcgca gctgcgccgc acatacgatc
     1801 ggtggcgttc cagcgggtgc gtccccgaga ccccggggac gtgctagaac tcggcgctca
     1861 tgcctagttc aggccgcccc aatcgaggca ggcggcggtg gcgtcgtcgt gcaaatctcc
     1921 cgctcgatgg gcgagaccac tcgtcaggca taccggtcac cgtacggggg tcccgcgagg
     1981 cgacaccgtg gcgcattgaa cgggacggcg gcggcatccg ttgtgggacc gagcacctca
     2041 ccacgacgtc gggagccaca gatgcgctca tctccaataa tcaaagcgac ggtcttcctc
     2101 ggcatggctg cactcttggt ggccggctgc gccagcaaga agtcgagcag ctctactccg
     2161 acccctgctg cgggggctag caccgccgcg gccgcggcac ccgccagcgc agcaccggcc
     2221 gccggcgggt caggagctgc cgccgcccca gcgggtccgg ccacggtcgc cgcgcagatg
     2281 ggtgcgctgg gcaactacct caccgatggc agcgggaaga gcctgtacct gtacacgcct
     2341 gacacgtcgt cgacctcgac gtgttacggg cagtgcatcg cgttctggcc gccgctgctg
     2401 accaccggtg cgccgaaggc gggaacgggt gcgaccgcca gcatgctcgg caccagtcct
     2461 cgcactgatg gcaccacgca ggtcacctac aacggtcacc cgctgtactc cttcaagggc
     2521 gacaaggccg ctggcgacac gagtgggcag ggcaaggcag gtacctggtt cctcgtgaca
     2581 cccgcaggta cgcagatcgc agccgcggcg tcgtccgccg ccgcaccgcc cgcatcgggc
     2641 aagtactgaa ctgacggcac cggttagcag ctgcaccggg cctgcgtgat caccagcaaa
     2701 tcggcgtccg gtcgcattga acgggacgcc gtccggagcc gtcctgggaa gcagcgccac
     2761 accaccgagc agcgtcccca accgagtagc acacatgtgc gggagtccca ggatgaagaa
     2821 cgcgatgcga agaacaacac cggcgatcaa ggcgacggct tacctt
//
LOCUS       DATCBJ010000356         2047 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_111342, whole genome shotgun sequence.
ACCESSION   DATCBJ010000356 DATCBJ010000000
VERSION     DATCBJ010000356.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2047)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2047)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2047
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_111342"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1240)
                     /locus_tag="VII50_07125"
     CDS             complement(<1..1240)
                     /locus_tag="VII50_07125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY5050657.1"
                     /translation="MSRTRLHVTTLLTDDSADTHQRRQAQTLQRARRNVDRDIRKTDR
                     TQARVGRRCAIASSNAVPAVGERRPQALRTWLPLRLPEHRASSARLAGAYPFLTGAAP
                     GAEGVPIGIDAYSGTTAAFDPWYLYGQGRLTNPNVLLAGVIGQGKSALAKSLAVRSVP
                     FGIQAYVPCDVKGEWAGVAKAIDGGVVLRIGLGSMTRLNPLDPGPKPNSVGVGEWQLA
                     VTARRRQLLNAIAETTLSRGLHPAERTALDVALEHAGVATDQVCLPAVVDALQAPPPQ
                     LARREHTTVAELAAAGSDVHHGLRRLVRGDLAGLFDGPSTAQLDTSAPMVVLDLSQLA
                     STGMATDLVGLVMTCASAWLESAIADPMGGRRWVIYDEAWRLLHIVPLVRRMQAQWKL
                     SRAYGTANLMVIHRLSDLDAA"
     gene            complement(1237..>2047)
                     /locus_tag="VII50_07130"
     CDS             complement(1237..>2047)
                     /locus_tag="VII50_07130"
                     /inference="COORDINATES: protein motif:HMM:NF042935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SCO6880 family protein"
                     /protein_id="HEY5050658.1"
                     /translation="VRDGSWAAHVVADLVATAPSRARHEVYLAVSVDVPRASGVGKAA
                     AGLERQMPAVRAAATRADLCVERWLDKAALGWVLRTAYDPSAAALTPVPVGAAAALAG
                     PVAVSEEWDRLRADGAFHAVYWITEWPREDTPAAFLQPLIFADATHALSIVATPQPTA
                     KAARDIRKAKADHHADAGQRARIGQVGDEGIDAEYDEVIARAKELAAGHTVLDFTGLL
                     TVTAVDDDQLDAACSEMEVAAAQAGCEIRRLVGQQAQAFIAAALPLARRIT"
BASE COUNT          299 a          671 c          747 g          330 t
ORIGIN      
        1 cggcggcgtc gaggtcggac agccggtgga tgaccatcag gttcgcggtc ccgtacgcgc
       61 gggacagttt ccattgggcc tgcatgcggc gcaccagcgg cacgatgtgc agtaaacgcc
      121 aggcttcgtc atagatcacc caacgccgcc cgcccatggg gtcggcgatc gctgattcga
      181 gccacgccga tgcgcaggtc atcaccaagc cgactaggtc ggtcgccatc ccggttgagg
      241 cgagctggga caggtccaaa accaccatcg gggctgaggt gtcgagctgc gcggtagacg
      301 gcccgtcgaa caacccggcc aggtctccgc ggacgaggcg gcgcaggccg tggtggacgt
      361 cgctacctgc ggcggcgagt tcggcgacgg tggtgtgttc gcgccgtgcg agttgcggag
      421 gcggggcttg cagtgcgtcg acgacagccg gtaggcaaac ctggtcggtg gcgacaccgg
      481 cgtgctcgag cgcgacgtcc aacgcggtgc gttcggccgg atgcaagcct cgactaaggg
      541 ttgtttcggc gatcgcgttg agtagttggc gtcggcgcgc ggtgaccgcc agctgccatt
      601 cccccactcc gacgctgttg ggcttgggtc ctgggtcgag ggggttcaac cgggtcatcg
      661 aaccgagccc tatccgcagc acgacaccgc cgtcgatggc cttcgcaacg ccggcccatt
      721 cacctttcac atcgcatggc acgtaggcct ggatgccgaa cggcaccgag cgcacggcga
      781 ggcttttcgc cagcgccgat ttgccttgtc cgatcacccc ggccagcaac acgttcgggt
      841 tggtgagtcg cccctgcccg tagaggtacc aggggtcgaa cgcggcggtg gtgcccgagt
      901 aggcgtcgat accgatgggc accccttccg cgccaggtgc ggcgccggta aggaacggat
      961 aggcaccagc gagccttgcg gagcttgcac ggtgctcggg cagccgcagc ggcaaccagg
     1021 tgcgtaatgc ttgtggtcgg cgctcgccga cggctggcac cgcgttactt gaggcgatgg
     1081 cgcaacggcg gccaacccgc gcctgcgtgc ggtcggtttt gcggatgtcg cggtcgacgt
     1141 tgcggcgggc acgctgcaag gtttgggctt gcctgcgttg gtgcgtgtcg gcgctgtcat
     1201 cggtcagcag cgtggtgaca tgcagacggg tgcggctcac gtgatccgcc gggccagagg
     1261 caatgccgcc gcgatgaaag cctgggcttg ctgcccgacc agacgacgga tctcacaacc
     1321 cgcctgagcg gccgccactt ccatctcgga gcaggccgca tcgagttgat cgtcatcgac
     1381 ggcggtgacg gtgagcagcc cggtgaagtc gagcaccgtg tggccggcgg cgagttcctt
     1441 ggcccgcgcg atgacctcgt cgtattcggc gtcgatgcct tcgtcgccga cctggccgat
     1501 gcgggcccgc tgcccggcgt cggcgtggtg gtcggctttg gctttgcgga tgtcgcgggc
     1561 cgccttcgcg gtcggctggg gggtcgccac gatcgacagc gcgtgggtgg cgtcggcgaa
     1621 gatcagcggc tgcaggaatg cggccggtgt gtcctcccgc ggccattcgg tgatccagta
     1681 cacggcgtgg aacgcgccgt cggcgcgcag ccgatcccac tcttcgctca ccgcaaccgg
     1741 gccagccaac gcagcggcag cgcctacagg gacgggcgtc aacgcggcgg cggacgggtc
     1801 gtaggcggtg cgcagcaccc accccagcgc ggccttgtcg agccaacgct cgacgcatag
     1861 atccgcgcgg gtcgcggcgg cgcggacggc gggcatctgt cgttccagcc cggcggcggc
     1921 tttccctacc ccgctagctc ggggaacgtc gacgctgacc gcaaggtaga cctcgtggcg
     1981 cgcgcgcgac ggcgcggtgg cgaccaggtc agcgaccacg tgcgcggccc aggatccgtc
     2041 gcggacc
//
LOCUS       DATCBJ010000357         3096 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_353180, whole genome shotgun sequence.
ACCESSION   DATCBJ010000357 DATCBJ010000000
VERSION     DATCBJ010000357.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 3096)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3096)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3096
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_353180"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..523)
                     /locus_tag="VII50_07135"
     CDS             complement(<1..523)
                     /locus_tag="VII50_07135"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family cysteine hydrolase"
                     /protein_id="HEY5050659.1"
                     /translation="MTQALLLMDIQNSILDRVGAGEPYLDKVVAVQERAETAGMLVVL
                     VRVAFRPGYPETSVRNKVFSGGPPGAGMVMGDPAAEPHPRLMRGNGEVVVTKKRISAF
                     AGSDLEVILRSRDVTHLVLAGISTSGVVLSTVREAADRDYELTVLEDLCLDADEEVHR
                     VLTSKVFVRQAGVI"
     gene            819..2096
                     /locus_tag="VII50_07140"
     CDS             819..2096
                     /locus_tag="VII50_07140"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEY5050660.1"
                     /translation="MATQSTEQLSLTGLQEFSEILESRQVRALFQPVVDLANGQTVGY
                     EALARGPVGSAYESPIALFAAAQRLDRISELDWVCRAAAFEAALTDGGSQLADAVPLF
                     LNCEPSAFGAPCPSDLQPLVHAALQRLHVVMEVTEREIARDPAGLLSAVAHARGGLWG
                     IALDDVGAEPDSMALMPFINPDVIKLDLRLIQARTTAEVARVVNAVLAQAERTGATIL
                     AEGIETPRHEQIARAMGATVGQGWLYGRPGPLPSTVPSPNRPLRLLDVEKPTSSQTPF
                     EIISARRQTTRSAKDLLIPMSMHLENKGLEGTEPVVLLACFQEAQHFTERARVRFERL
                     AKSASFVGAVGAGMGSTPAAGVRGANLTAADPLRGEWDVIVVGPHFAGALVARDCGDI
                     GPDLLRRFDACITYDRLLVIEAARALLSTVLRA"
     gene            2156..2470
                     /locus_tag="VII50_07145"
     CDS             2156..2470
                     /locus_tag="VII50_07145"
                     /inference="COORDINATES: protein motif:HMM:NF015513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEY5050661.1"
                     /translation="MQRNRPPSAQTINVLQALAADPAQWRYGYDLATEVQLKSGSLYP
                     ILVRLADRGMLETAWEQGEGSRPPRHMYRLTTAGREYVTALPAVRARKTVPRAHKRLS
                     EA"
     gene            2473..>3096
                     /locus_tag="VII50_07150"
     CDS             2473..>3096
                     /locus_tag="VII50_07150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5050662.1"
                     /translation="MLIIPIMLLGICVALIRRSSHAAVEGPPGDAPMRLLRWATGMLS
                     AQRAEWGQAMLGELDHIDGRARRWRFTIGCVGAALLLSPWERAGAAALAMVALGIGGL
                     GVYAFVIIRYGLGAGSLVGAAIVAAFLLSFVLAAVVLLRRIGVAGPGLLGGLFVAVVW
                     LAMSGFTFLNLIARVWPAWALPLLFIVAPGVVGVLGTLRGGSAVAGRR"
BASE COUNT          484 a         1017 c         1028 g          567 t
ORIGIN      
        1 cgatgacgcc ggcctgccgg acgaacacct tgctcgtgag tacacgatgg acctcttcgt
       61 cggcatccag gcaaaggtct tccagcaccg tgagctcgta gtcgcggtcc gcagcctcgc
      121 gcacggtcga gagcacaacc ccgctcgtcg agatccccgc gagcacgagg tgagtcacgt
      181 cacgtgatcg cagaatcact tccaggtcgc tgccggcgaa ggcgctgatt cgtttcttgg
      241 tgacgacgac ctcaccattg ccccgcatca aacgcgggtg cggctcggcg gccggatcgc
      301 ccatgaccat gcccgccccg ggcgggcccc cggagaacac cttgttacgc acggaggtct
      361 cgggatagcc gggacgaaaa gcgacccgca ccagcaccac gagcatgccg gcggtctcag
      421 cacgctcctg aaccgcgacg actttgtcga ggtagggctc gcccgcaccg actcggtcaa
      481 ggatgctgtt ctggatgtcc atcaatagca gcgcttgagt catgctcccg atcctgcacc
      541 acgaccgcca gcggcctaga gagacatctg cagttcggcg agacgacgag gtacccgcgt
      601 tcgtcaacga cctcgaacgg cacctatttc tccgtcccgt cgtggtggga agcggcactc
      661 attggctgca ccacgacgtc cgcctgaatg ttgaattgct cgatgagcgc cgctccgacg
      721 gcggcgtcgt ccacctccac taccgagtgt cgagctcggc ttatgcgaat cgcttgggga
      781 cctctcatct atgaggtggc ttggccgata gggccggtat ggccacacag agcaccgagc
      841 agctttcgct gactggtctg caggagttct cggaaatcct cgagtcgcgc caggtgcgag
      901 cactgttcca gcccgttgtc gacctcgcga acggtcaaac agtcggctac gaggcgttgg
      961 ctcgcggccc ggtgggctcg gcgtacgaga gcccgatcgc gctgttcgcc gcggcacagc
     1021 gtctcgaccg aatctctgag ctggattggg tgtgccgcgc ggccgcgttt gaagcggcgc
     1081 tcaccgatgg cggctcccag ttggcggacg cggtaccgct ctttctcaac tgcgaaccgt
     1141 cggcgtttgg ggcaccgtgt ccatcagacc tccagccact tgtgcacgcg gcgctgcaac
     1201 gcctgcacgt ggtgatggaa gtcaccgaac gcgagattgc gcgcgacccc gccggtttgc
     1261 tgtcggcggt ggcgcatgcc cgtggcgggt tgtggggcat cgcccttgac gatgtcggtg
     1321 cggagcccga cagcatggcc ttgatgccgt tcatcaaccc cgacgtcatc aagctcgatc
     1381 tgcggttgat tcaggcgcgg acgacggccg aggtcgcaag agtcgtcaat gcggtgttgg
     1441 cacaggccga acgaaccggg gccacgattc tcgccgaagg catcgaaaca ccgcgccacg
     1501 agcagatcgc cagggcgatg ggcgcaacgg ttggccaagg ttggctctac ggacgccccg
     1561 gtcccttacc gagcaccgtg ccttctccga accgcccgct tcgcttgctc gacgtcgaga
     1621 agccgacctc gagtcagaca ccctttgaaa tcatcagcgc ccgacgccag acgacgcgct
     1681 cggccaagga cctgctcata ccgatgagca tgcatctgga aaacaagggc ctcgaaggca
     1741 ccgagccagt cgtgctcctc gcttgcttcc aagaggcgca gcatttcacc gaacgcgctc
     1801 gtgtgcgatt cgaacggctg gcgaagagtg ccagcttcgt cggtgccgtg ggtgccggaa
     1861 tgggctcgac acccgcggcc ggagtgcgcg gtgcgaacct cacagcggcc gacccgctcc
     1921 gcggagagtg ggatgtgatc gtcgttggcc cgcacttcgc cggcgcgctc gtcgcgcgcg
     1981 actgtggtga tatcgggccc gacctgctgc ggcggttcga cgcgtgcatc acgtacgacc
     2041 gcctgttagt aatcgaagcc gcccgcgcgc tgctgtcgac cgtgctccgg gcatgatcgc
     2101 gcgatacggg tgcggctctt cttgaccgtt actgctacat gagtagactt aagtcatgca
     2161 acgcaaccgc ccgccctccg cgcagacaat caacgtcctg caggcgttgg ctgccgaccc
     2221 ggcgcagtgg cgctacggct acgacctcgc gactgaggtg caactgaagt ccggctcgct
     2281 ctacccgatc ctggtccggc tcgccgaccg cggcatgttg gagaccgcgt gggagcaagg
     2341 cgagggaagt cggccgcccc ggcatatgta ccggctgact acggcgggcc gcgagtacgt
     2401 gacggcattg cccgccgtcc gcgcccgcaa gaccgttccg cgcgcgcaca agcgcttgag
     2461 tgaggcgtag ccgtgctgat catcccgatc atgctgctcg gtatctgtgt ggcgctgatc
     2521 cgacgttcat cgcacgccgc cgtggaaggc ccgccggggg acgcgccaat gcggctgctg
     2581 cggtgggcga ccggcatgct gtccgcgcag cgagccgagt ggggccaggc gatgctcggt
     2641 gagctcgatc acatcgacgg tcgcgctcgg cggtggcgct tcacgatcgg ctgcgtcggt
     2701 gcggcactgc tgctctcgcc gtgggagcga gccggcgctg ctgcgttggc gatggtcgcg
     2761 ctgggtatcg gcggcctcgg tgtgtacgcg ttcgtcatca tcagatatgg gctgggcgcc
     2821 ggtagcttgg ttggcgcggc gatcgtcgcg gcgttcctgc tgagcttcgt cctcgccgcc
     2881 gtcgtccttt tgcgccgcat cggtgtcgcc gggcccggac tgctgggcgg cctgttcgtc
     2941 gccgtcgtct ggctggcgat gtctgggttc accttcttga acctcatcgc cagagtgtgg
     3001 ccggcgtggg cgcttccgtt gttgttcatc gtggcgccgg gggtcgtcgg agtgctcggt
     3061 accttgcgtg gcggtagcgc cgtcgcggga cgacgg
//
LOCUS       DATCBJ010000358         3974 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_92293, whole genome shotgun sequence.
ACCESSION   DATCBJ010000358 DATCBJ010000000
VERSION     DATCBJ010000358.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 3974)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3974)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3974
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_92293"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(81..1400)
                     /locus_tag="VII50_07155"
     CDS             complement(81..1400)
                     /locus_tag="VII50_07155"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEY5050663.1"
                     /translation="MEVVRRRVAVVTTEPLSAELAGPAIRAVELGRVLASEHDVEVAS
                     TASCSESGPDGNWQFVDDAALRALAARCDVMVLGGDVLAAHAWLADAGPAIVVDLYDP
                     FHLEQLEQARDLGEAERRRVVFGCIDVLNTQAVLGDTFLCASGRQRDMWLGHLAACGR
                     VNPATYDADPTLRTLLRVVPFGVADQPVQVGAPALRGVVPGIGVDDEILLWGGGIYNW
                     FDPETLIRAVDKLRADRPKLRLVFMGVQHPNPVVKTMRAAAQAWELSNELGLTDVHVF
                     FSDSFSSGWIPYAERGRYLAEATIGVSTHHDHVETAFSFRTRILDYFWAGLPVVTTRG
                     DALAEVIERAGAGRTVDPGDVDGLASAIAELLDDEAARESAAAASSRLADDYRWSTVA
                     QPLLEFCRNPYRAPDLADPALRAAILGRARSAPTKSLGARVASRLRK"
     gene            complement(1406..>3974)
                     /locus_tag="VII50_07160"
     CDS             complement(1406..>3974)
                     /locus_tag="VII50_07160"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEY5050664.1"
                     /translation="RVTAAHYAIAESDADFVILLDAGDRLVRDCLYQVARSAWQDPFV
                     DLVTWDDDLLGRDGRRHDPRFRPSWSPESMLGAGYLGQSFAIRRRRAMFAGNLRNTYG
                     GATRWDLLLRSDLDAERVVRVPRVLAHLRQRRQGLNADGVRVVQEALDGRGVPATAEL
                     AEHSVRLRWQLESWPHVTIVIPTRHNRPMLSKVLPGLAATDYPSFDLRIIDNGGQSEA
                     NDAWYAEHAAGLDLHVQWWTETPFNYSRVNNEAAAGARGEMLVFLNDDIELPDPSWLR
                     EIVGWATRPEIGVAGLQLTRADGKIQHAGVILGMGGFADHVFEGMEPGSDSLLGPTDW
                     YRNVLAVTGACLAIRRETFDRVGGFDERFILCGSDVALGLDTTQLGLRNVCSPFGGVR
                     HLESATRGSQIPTEDFFASYWRYNTWLFGTGDPYFSPNLSLSSRKPKLRGLQEPTPAS
                     RLAGPLGRDLQVFRQRSDAAESTMLADTCRISDVDVAAIANLHAANAAHLDVTTINWF
                     IPDIDSPFYGGINTALRLADYLHRNHGVQNRFVVWGSGPDFFVRSAITAAFPSLADSQ
                     ISFFDGPDSASLELVPPADASIATLWLTAYAVGRAQNTKRKFYLVQDFEPMFYPASTS
                     YALAEETYRLGLYALCNTENLRQIYADDYGGKGMSFMPAVDPSVFHAHDRPFRSDEAP
                     TTVFVYARPGHWRNCWELASLALRELKERLGDGVRVVTAGSWATDPDAADAIKQLGLL
                     PYKATGELYRHCDVGLALTVSKHPSYLPLELMACGVPVVAFDNSWGHWILKDGENSLL
                     ARRTVTGLVDAMERMVVDPELRRRLASNAVADIAAHHGSWDAAFRGIYDYLCDPEKH"
BASE COUNT          712 a         1293 c         1356 g          613 t
ORIGIN      
        1 tggcggggcg ctggtcgtca acggccgttc ttgcgcccga tggagaccaa tggttcgccg
       61 gtggcgccgg cagtccctgg ctacttgcgg agcctcgagg cgactcgcgc gcccaagctt
      121 ttggtcgggg cggaacgcgc tcggccaagg atcgccgcgc gcaacgcggg gtcagcaagg
      181 tcgggtgctc gataggggtt gcggcagaac tccagcaacg gctgggcgac ggtcgaccac
      241 cggtagtcgt cggcgagtcg ggaactggcc gcggcggctg attcgcgcgc tgcctcgtcg
      301 tccagcaatt cggcgatcgc ggacgcgagc ccgtcgacgt cccccggatc gaccgtgcga
      361 cccgcacctg cacgctcgat gacctctgcc agcgcatcgc cacgcgtcgt gaccaccggc
      421 aggccggccc agaaatagtc gaggatccga gtgcgaaacg agaacgcggt ctcgacgtgg
      481 tcgtgatgcg tgctgacgcc gatcgtggct tcggccaggt agcggccgcg ctcggcgtac
      541 gggatccagc ccgacgagaa cgaatcagag aagaagacgt ggacgtcggt cagcccgagc
      601 tcgttggaca gttcccacgc ctgggcggcg gcacgcatcg tcttgacgac cgggttcggg
      661 tgctgcacac ccatgaacac caaccgcagc tttggtcgat cggcccgcaa tttgtccaca
      721 gccctgatca gggtctcggg atcgaaccag ttgtagatgc cgccgcccca caacagaatc
      781 tcgtcatcga cgccaatgcc ggggaccacg ccgcgaagcg caggagcgcc aacctgcacc
      841 ggctggtcgg cgacgccgaa cggcaccacc cgaagcagcg tgcgaagggt cgggtcggcg
      901 tcataggtcg ccgggttgac gcgcccgcac gcggccaaat ggccgagcca catgtcgcgc
      961 tggcgtccgg acgcgcacag aaacgtgtcg ccaagcacgg cctgcgtgtt cagcacgtcg
     1021 atgcagccga agaccacgcg ccgacgctcg gcctcgccga gatcgcgcgc ttgctcgagt
     1081 tgctcgaggt ggaacgggtc gtagaggtcg acgacgatgg ccggcccggc gtcggccaac
     1141 cacgcgtgcg cggccagcac gtcgccgccg agaaccatga cgtcgcagcg cgctgcgagt
     1201 gcgcgcaacg cagcgtcgtc aacgaactgc cagttcccgt cggggcctga ctcgctgcat
     1261 gatgcggtcg aggcgacctc gacatcatgc tcggacgcga gaacgcgccc gagttcgacg
     1321 gcacggatag cgggcccggc gagctccgca ctcaacggtt cggtcgtgac gaccgcaacc
     1381 cggcgcctca cgacctccac cggcgtcagt gcttctcggg atcgcacagg tagtcgtaga
     1441 tgccgcgaaa cgcagcgtcc caactcccat ggtgagcagc gatgtcggca acggcattcg
     1501 acgcgagcct gcgacgcagc tccgggtcga cgaccatgcg ctccatcgca tcgaccaagc
     1561 ccgtgaccgt gcgtcgtgcg agcagcgagt tctcaccatc cttcagaatc cagtgccccc
     1621 acgagttgtc gaacgccaca acgggcacac cgcacgccat cagctcgagc ggcaggtacg
     1681 acgggtgctt cgagacggta agcgcgaggc cgacatcgca gtgccgatag agctcgccgg
     1741 ttgctttgta cgggagtagc ccgagctgct tgatggcgtc ggcggcatcg ggatcggtgg
     1801 cccaagagcc ggcggtaaca acgcggacgc cgtcgccgag ccgttccttc agttcgcgca
     1861 gcgctagcga cgcgagttcc cagcagtttc gccagtggcc aggccgggcg tagacaaaca
     1921 cggtggttgg cgcttcgtcg gagcggaacg gccggtcgtg ggcgtggaac accgaagggt
     1981 cgaccgcggg catgaaactc atgcccttgc cgccgtagtc gtcggcgtag atctgccgca
     2041 ggttttcggt gttgcacagc gcgtagaggc cgagccggta ggtctcctca gcaagcgcgt
     2101 acgacgtgct cgccggatag aacatcggct cgaaatcctg caccaggtag aatttccgtt
     2161 tcgtgttctg cgcccgcccg accgcatatg cggtgagcca cagcgtcgcg atcgacgcgt
     2221 cggccggcgg cacgagttcg agactcgcgc tgtcgggtcc gtcgaagaag ctgatctgac
     2281 tgtcggcaag agacgggaag gctgcggtaa tcgccgagcg cacgaagaag tcaggcccgc
     2341 tcccccacac cacgaaccgg ttctgcacgc cgtggttgcg gtgcaagtag tcggccaacc
     2401 gaagtgccgt gttgatgccg ccatagaacg gtgagtcgat gtcggggatg aaccagttga
     2461 tcgtggtgac gtcgagatgt gctgcgttcg ccgcgtgcag gttcgcgatc gcggcgacat
     2521 caacatcgct gatccgacac gtgtctgcga gcatcgtaga ctcggcagcg tcgctgcgct
     2581 gccggaacac ctggagatcg cgtcccagtg ggccggccag ccggctggcc ggagttggct
     2641 cctggaggcc gcgcaatttc ggcttgcggc tcgacaacga caggttcggt gagaagtacg
     2701 ggtcgccggt gccgaacagc cacgtgttgt agcgccagta gctggcgaaa aagtcctcgg
     2761 tcgggatctg agaaccccga gttgctgact ccaggtgccg cacgcccccg aacggcgagc
     2821 agacattgcg caacccgagc tgcgtggtgt cgaggccgag cgcgacgtca ctcccgcaca
     2881 agatgaaccg ctcgtcgaag ccaccgacgc gatcgaacgt ttcccgccgg atcgccaggc
     2941 atgcgccggt caccgcgagc acgttgcggt accaatcggt gggcccgagc aaggaatcgg
     3001 agcccggttc catgccctcg aaaacgtgat cggcgaagcc acccatgccg agaatgacgc
     3061 cggcatgctg gatcttgccg tcagcgcgag tgagctggag cccggcgacg ccgatctcgg
     3121 gcctggttgc ccagccgacg atctcgcgca gccacgaagg gtcgggcaac tcgatgtcgt
     3181 cgttgaggaa gacgagcatc tcaccgcggg cgccggccgc ggcctcgttg ttgaccctgg
     3241 agtagttgaa cggagtctcg gtccaccact ggacgtgcag atcgagccca gctgcgtgtt
     3301 cggcgtacca ggcgtcgttg gcctcggact gaccgccgtt gtcgatgatg cggaggtcga
     3361 acgacgggta gtcggtggcc gcgagaccag gcagcacctt cgacagcatc ggccggttgt
     3421 gccgggtcgg aataacgatg gttacgtgcg gccagctctc aagctgccag cgcagacgca
     3481 cggaatgctc ggcaagctct gcagttgcag gaacgccgcg gccgtcgagc gcttcttgta
     3541 cgacgcggac gccgtcggca ttgaggccct gccgtctctg ccgcaggtgg gcgagaacgc
     3601 gcgggacccg gacgacccgc tcggcgtcga gatcagagcg caacagcagg tcccagcggg
     3661 tggcgcctcc gtaggtgttg cgcaggtttc cggcgaacat tgcgcgccgc cgccggatcg
     3721 cgaacgactg accgaggtag cccgcgccga gcatcgactc gggcgaccac gacggccgga
     3781 atcgcggatc gtggcggcgg ccatcgcggc ccagcaaatc gtcgtcccat gtcaccaggt
     3841 cgacgaacgg gtcctgccag gctgaccgag cgacctgata gaggcagtcc ctgaccagcc
     3901 ggtcacccgc gtcgagcagg atcacgaagt cggcgtcgga ttcagcgatg gcgtagtgag
     3961 ccgccgtcac gcgg
//
LOCUS       DATCBJ010000359         2068 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_479826, whole genome shotgun sequence.
ACCESSION   DATCBJ010000359 DATCBJ010000000
VERSION     DATCBJ010000359.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2068)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2068)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2068
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_479826"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            1228..>2068
                     /locus_tag="VII50_07165"
     CDS             1228..>2068
                     /locus_tag="VII50_07165"
                     /inference="COORDINATES: protein motif:HMM:NF017416.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEY5050665.1"
                     /translation="MLTLWAGQPECLWDEALPIEVRELPQDLAALDRLLSDPELAMVF
                     LERWRREVVETGRLVLTDGRPAIAMETYVRLMVLKARYRWGYRTLVAEVSDSIHLRRF
                     CRISLSERVPDESTIRKLTRRIGAETVNELTRMLIVEATRSRRFRARAVRIDSTVVAA
                     DVKYPTDAGLAAHGVRALAREGRKLAKLVKKPKLRVRDRSRAMGVKLRAVTRTIRRRS
                     GEAKSEVLKLTEQTGELLTRSVTEARKLAAAARRHARGRGAKTKLNAAGRLEQLADRC
                     VKVS"
BASE COUNT          368 a          619 c          713 g          368 t
ORIGIN      
        1 gtgtcagcgg gtgcgcgttc ggcgagcatt tcgattggct gtcttagtag aacgagtgca
       61 tcaggcacgc tacgttcgcc agtgctgcca cctgaactgt cccgcggcta actccatcca
      121 cgcgctatct gaagagaccg ggtctgctcc tggaccaacc ccgacgcgat ccgaaccaca
      181 accgccgcca cgctgaccca gcaccccctc tgttaggcaa gccagcccga acatacacct
      241 cgtgacagcc agggccgctt gcgttgcccg cacctctgta tcccgacaac cttgcaaagg
      301 aatagggagg tttgtaacgg gatacttacc ccacgtaggc ccgaagtcaa gcaggcctaa
      361 cacagcgatg agactcccgc gcttcgccgg gcgtcacctg ggtgacgggc tctacacgca
      421 acggtacgcc gcggtgcttg ctgactctgg ctagcactcg gctcgaaggg gcgaactcaa
      481 gctggtcgtc caagccgaca aaacgtggca ggagcgtctg cgtgccgagc tgcggcacga
      541 gtcctttgcc acgaactcga tcgcgcggcc ccgtcgatca cgtaaccgtt cgacgagcga
      601 agcagacatc acgcacatgc tgaaattgcc gacgcgcgtt gtcgggcccg gacacgatgg
      661 gtcacatacg tgtcgccgat ttcatcgccg acggcggacc gacggcgacg gcgccgtggg
      721 acgctacccc tcgacgcctc aagcagccat ggcgaagttg tgaccgcttc gccccgacga
      781 gcagccaccg tgcggccgcg gcgaagctgc cctgcttggc tactgttcaa cttcgatcag
      841 ccgtttgcat cttcacccca cgcgcgcgcc gatgttacgt cctcttcgtc ttcttgggct
      901 tgaaggtgat ggtcgagcta ccggtcgtct tgcctgactg cgtgatcgcc agcttcgccg
      961 tcagcgggcc gtggataccg gcccagctcc gaacccgcag cccgcgcgag accacacaac
     1021 accgacttgt cgggctgggg cggagcccgg ttagcaattc acccaacgta ggagcgcagg
     1081 ctagtactag taattcaccc aacgcaggag cggattagat tacttcctgc ggaaaaagta
     1141 gaggctgttc gagggcctgg ttccgtccgg tcggattgac aaaatccctg ctagtggatg
     1201 ggtttgtggt cgatctgagg aggacgtgtg ctgacgcttt gggcggggca gccggagtgc
     1261 ttgtgggacg aggcgctccc gatcgaggtg cgcgagttgc cgcaggactt ggcggcactg
     1321 gaccggttgt tgtccgatcc ggagttggcg atggtgttct tggagcgctg gcgtcgggag
     1381 gtggtcgaga ccggccggtt ggtgttgacc gacggccggc cggcgatcgc gatggagacg
     1441 tacgtgcggt tgatggtgct caaggccagg tatcggtggg ggtaccggac gctggtggcg
     1501 gaggtgtcag attcgattca tctgcgccgg ttctgtcgga tcagcctgtc agagcgggtg
     1561 ccggacgagt cgacgatccg gaagctcacg cgccggattg gtgcggagac ggtgaacgag
     1621 ctgacgcgaa tgttgatcgt ggaggcgacc cggtcgcggc ggtttcgggc gcgggcggtg
     1681 aggatcgatt cgacggtcgt ggctgcggat gtgaagtacc cgaccgatgc tgggctggcg
     1741 gcgcacggtg tcagagcact cgcgcgcgag ggtcgcaagc tcgcgaagct ggtcaagaag
     1801 cccaagctgc gggtgcggga tcgctcgcgg gcgatggggg tcaagctgcg ggcggtgact
     1861 cgcacgattc gtcgccgatc aggcgaggcg aagagcgagg tcctgaagtt gaccgagcag
     1921 accggcgagc ttttgacacg ctcggtcacc gaggcacgga agctcgcggc ggccgcgagg
     1981 cgccacgcgc gtgggcgtgg cgcgaagacg aagctgaacg cggccggccg gctggagcag
     2041 ctcgccgatc ggtgcgtgaa agtcagcc
//
LOCUS       DATCBJ010000360         2238 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_11685, whole genome shotgun sequence.
ACCESSION   DATCBJ010000360 DATCBJ010000000
VERSION     DATCBJ010000360.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2238)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2238)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2238
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_11685"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..709)
                     /locus_tag="VII50_07170"
     CDS             complement(<1..709)
                     /locus_tag="VII50_07170"
                     /inference="COORDINATES: protein motif:HMM:NF015155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HEY5050666.1"
                     /translation="MTGVLYRLARFCVRRRFVVVGVWLVVAIGLVAVSHRLGDNTNDN
                     LSLPGTGSQHATDTLTRSFPDQANGTSPIVLHASSGKLSDSKYSSAVNEAATDVAKAP
                     NVASVLNPLTPQGASSLSKDQSTGYLSVTLSVSPGSLSVGDVQRIVDAANPAKKAGLE
                     VETGGQLGQKISKTSTESSELIGIIAAMVILTLTFGTVVSMLLPILNAILGLLATLAI
                     IRMLGHVATVPTVAPTLA"
     gene            complement(1249..>2238)
                     /locus_tag="VII50_07175"
     CDS             complement(1249..>2238)
                     /locus_tag="VII50_07175"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEY5050667.1"
                     /translation="RSPRGRELIEACITAAGAFLTNFPARGWLSYESLSSRRADLVMV
                     LLTGNRDGTSEVDYTVNPATGFPWATGPRDYAEPLNSVLPAWDAITGGLATTGLLAAD
                     RHRTRTGEGQLVRVALSDVAFAMVGHLGRVAEAQLGSRDTPKDGNYLYGAFGHDFATS
                     DGRRVMVVALTTRQWNSLLEVTGLAATVDAIEQAMGMELQSVSDRFQARDLLAAVLQP
                     WFEARTLTEIRAAFKGTGVSWGAYQTFSQLVREDPRCSTDNDMWEMVQHPGVGSYLMP
                     GTPLDFSAVARVPVRRAPRLGEHTEAVLAELLSMTTAEIGRFERDGIVRCARG"
BASE COUNT          363 a          714 c          792 g          369 t
ORIGIN      
        1 tcgcgagcgt cggcgcgacg gtcggcacgg tcgcgacatg cccgagcatg cggatgatcg
       61 cgagcgtcgc gagcaagccg aggatcgcgt tgagaatcgg caagagcatc gagacgacgg
      121 tgccgaacgt cagcgtgagg atgaccatcg cggcgatgat cccgatcagc tcgctcgact
      181 cggtggaggt cttggagatc ttctgaccga gctgccctcc ggtctccacc tccagtccgg
      241 ctttcttggc cggattggcg gcatcgacga tccgctgcac gtcgccgacc gacagtgagc
      301 ccggactcac cgacagcgtt actgacagat aaccggtgga ttggtccttg ctgagtgagg
      361 aagcaccctg gggcgtgagc gggttgagca ccgaagccac gttcggcgcc ttggccacat
      421 cggtcgccgc ctcgttcaca gcgctcgagt acttcgagtc actcagcttg ccgctcgaag
      481 catgcagaac gatcgggctg gtgccgttgg cctgatcggg gaaggacctg gtcagggtgt
      541 cggtggcgtg ctggctgccg gtcccaggca acgataggtt gtcgttggtg ttgtcaccga
      601 ggcggtgcga caccgccacg agcccgatcg ccaccaccag ccagacgccc accaccacga
      661 accgccgccg gacgcagaac cgcgccagtc gatagaggac cccggtcaca gccggttgcg
      721 tgccggaggt ggatcgaggg gagtgcgagc ggcgagcagg agatctccga acacgcgcct
      781 gcgctcgagc gcgcgtgatg ggccacggcc gtcgtcacta ggcgccaagc gaacggcgcc
      841 gctaggaagc tcgtcgtcgc gtcgcggtac ccgcatccag cctcgttgcc ccctctccgg
      901 ttcgctgcag aacccagcga cagcatgcca cagacttccc gccagcgcac ggagccccct
      961 acagccctgc ggcgattgtc ggttgctcac ggaagtgcga atatcaccgg cgtgccaatg
     1021 gtgcgctcaa ccggtccggc tgcagtgtca ggctcgacgg agggcatgtg ctgggtgcca
     1081 ggcggcgagt ttcggatggg tgcggacgat ttctatcccg aggagctgcc ggttcgcagg
     1141 gtcttcgtcg gtcgggtcgt agaagtaggc ggccttcacg gtcgccggcg aaccgacgga
     1201 gacggtcggc gatatggacg attcgacggc ccttcggggt cgctgtgctt agccgcgagc
     1261 gcagcggaca atgccgtcgc gctcgaaacg gccgatctcg gcggtcgtca tgctcagcag
     1321 ctccgcgagt acggcttctg tgtgctcgcc gagtcgcggc gcgcggcgaa cggggacgcg
     1381 cgcgacggct gagaagtcca gcggtgtccc gggcatcagg tagctgccca cgccggggtg
     1441 ttggaccatt tcccacatgt cgttgtcagt cgagcagcgt gggtcctcgc gcacgagttg
     1501 cgagaacgtc tggtaggcgc cccaggacac gccagtgccc ttgaacgccg cgcgaatctc
     1561 cgtcagcgtg cgagcctcga accatggctg gagcaccgcg gccagcaggt cacgggcttg
     1621 gaagcggtca gagacggact gtagttccat gcccatcgcc tgctcgatcg cgtcgacggt
     1681 ggcggcgagt ccggtgacct cgagcagcga gttccattgg cgggtggtaa gcgctaccac
     1741 catcacacgg cgcccgtcgc tcgtggcgaa gtcgtgtccg aaggctccgt agaggtagtt
     1801 gccgtccttc ggtgtgtcgc ggctgccgag ctgcgcttcg gccacgcgcc cgaggtgacc
     1861 gaccatcgcg aacgcgacgt ctgacagggc gacgcgcacg agctgcccct cgcccgtcct
     1921 ggtgcggtgg cgatcggcag ccagcaggcc ggtcgtcgcg agtccgcccg tgatcgcatc
     1981 ccacgcgggc agcacgctgt tgagcggttc ggcgtagtcg cgcggtccag tggcccaggg
     2041 aaagccggtc gcggggttga ccgtgtagtc gacctcggag gttccgtcgc ggttgccggt
     2101 gagcaggacc atgacgaggt cggcgcggcg gctcgagagc gactcatagg agagccagcc
     2161 gcgggcgggg aagttcgtca aaaacgcgcc cgccgcggtg atgcacgcct ctatgagttc
     2221 gcgtccgcgg ggtgagcg
//
LOCUS       DATCBJ010000361         6066 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_113696, whole genome shotgun sequence.
ACCESSION   DATCBJ010000361 DATCBJ010000000
VERSION     DATCBJ010000361.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 6066)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6066)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6066
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_113696"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(14..382)
                     /locus_tag="VII50_07180"
     CDS             complement(14..382)
                     /locus_tag="VII50_07180"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY5050668.1"
                     /translation="MTATMVVATIPVTDLDRSKKFYGETVGLTFLWENPFSARYRCGP
                     ASELSIFKRPPVATEHTLAHFEVDDIEAEVKNLEAGGVEFLDYTEGPLATTGHIAQLG
                     PARGAWFHDPDGNTLGIRQA"
     gene            complement(385..693)
                     /locus_tag="VII50_07185"
     CDS             complement(385..693)
                     /locus_tag="VII50_07185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5050669.1"
                     /translation="MSTKPYGVVHFFPAGTAEQYEASIAAVHPADGQLPAGQLFHAAG
                     ASPGGWTILAVHETLGSWEEFRDTILLPRMQQGIDGGFTSPPQETTIDVYKVMSGGAF
                     "
     gene            782..1240
                     /locus_tag="VII50_07190"
     CDS             782..1240
                     /locus_tag="VII50_07190"
                     /inference="COORDINATES: protein motif:HMM:NF024212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HEY5050670.1"
                     /translation="MGTDRADSVERWTPAWLSLVRTHARLWDQVEARMRRDSGLTMPR
                     YDVLMHLEMAGGRLGLTDLAAAIVLSPSGLSKLLDRMEVSGLLRREADPHDARSTFAT
                     ITPRGKTVVKKARQSHHAWLQQLFGDALDNRDLADLTRVMDRIDMRAPRI"
     gene            complement(1265..2233)
                     /locus_tag="VII50_07195"
     CDS             complement(1265..2233)
                     /locus_tag="VII50_07195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005454992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HEY5050671.1"
                     /translation="MLAASVMSFDAADPLVGLTVGEVQNPSPPDGWARVHVKAAALNQ
                     HDVWSLRGVGLRADELPRVLGCDAAGFDDDGNEVVVHGVIGDPDAGRGDESMDPNRSL
                     LSERFDGALAEFVVVPARNLLPKPAGLSFEEAACLPTAWLTAYRMLFTNSGTRPGDTV
                     LVQGAGGGVATALIALAKAAGRRVWVTSRSLDRGGLALKIGADAAYESGGRLPERVDA
                     VFDSVGAATWAHSLRCLKPGGTIVTCGATSGDAPSAELSRVFFQQLRVIGSTMGTRQE
                     LVELLNFLMVTGVRPTIDRILPLAQAPEGFAAMVAGDVFGKVVFTV"
     gene            2460..3425
                     /locus_tag="VII50_07200"
     CDS             2460..3425
                     /locus_tag="VII50_07200"
                     /inference="COORDINATES: protein motif:HMM:NF012707.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY5050672.1"
                     /translation="MTPLRLRPRTQAAGIVAIATMLALTGCSSKKSTAASSPSSAAPS
                     AASAAPSTAAAPSAAASSAADPTSLLPAAIKSAGVLNIATDASYAPNEYFDTDGKTII
                     GLDVDLGNAIAAKLGLKANFQNVTFNAIIPALAGGKYDLSLSSFTDTKAREATVDFVD
                     YFNAGESLMVLAGNPKGVTGPDLSLCGLTIAVEKGTTEESEIPALSKKCTDAGKKAIT
                     GLSSDDQNGANLALTSGRADVGSADSPIAAYAVKQLAGKIALAGQTYETAPYGIAIPK
                     TPGLAPAIKAALAAMLADGSYKTILDKWGDDAGAITTPVINGATS"
     gene            3433..4386
                     /locus_tag="VII50_07205"
     CDS             3433..4386
                     /locus_tag="VII50_07205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009080627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEY5050673.1"
                     /translation="MTSGGDVSVTPDNVVRGRPENIRAVPVRHPWRWVAIAVIAVLAA
                     MLVHMLFTNPNLEWSSVRKYLFSNIILRGVWMTIKLTILSMAIGIVLGVVAAVMRLSE
                     NPVVRGAAWLYVWFFRGTPLLVQIIGWFSISLLFKTLAFGIPFGPHFAGENTNKLVSL
                     FVSALLALSLNEGAYMSEIVRAGILAVDEGQTEAAHALGMTKLQTMRRIVLPQAMRVI
                     VPPTGNETNGMLKNTALASTVGIFELLRSSEAIYNANYKTIGLLIVAVIWYLAMTSIL
                     QVGQFYLERYYGRGSTRTVALTPIQRARLALMGNHGRGGAN"
     gene            4395..5147
                     /locus_tag="VII50_07210"
     CDS             4395..5147
                     /locus_tag="VII50_07210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003931904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEY5050674.1"
                     /translation="MVDAQQVHKSFGPIEVLKGIDFHVAPGQVVCLLGPSGSGKSTFL
                     RCINHLEKINAGRLFVDGELVGYRQHGDKLHELSAKEVAKKRTEIGMVFQHFNLFPHM
                     TALGNIMEAPCQVKNEPKAEVRTRAIELLAKVGLGDKASAYPRQLSGGQQQRVAIARA
                     LAMRPKLMLFDEPTSALDPELVGDVLAVMKDLASSGMTMIVVTHEIGFAREVADTVVF
                     MDGGVVVEQGPPSEVISNPRHERTKAFLSQVL"
     gene            5171..5779
                     /gene="rfbC"
                     /locus_tag="VII50_07215"
     CDS             5171..5779
                     /gene="rfbC"
                     /locus_tag="VII50_07215"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HEY5050675.1"
                     /translation="MEVRELAVPGAWEFTPRQIGDERGVFLEWFRADVVNRTIGHSLE
                     IAQCNQSVSRRGTVRGIHYADVPPSQAKYVYCPGGAVLDVIVDIRAGSPTFGAVDTVR
                     LDNVDRRAVYLSEGLGHAFMALTDGATVTYLCSTGYAPDREHGVNPLDPALAIPWPDN
                     LEPVLSDKDRDAPSLAEAAEQGVLPTYAECLAYYASLHRARG"
     gene            5809..>6066
                     /locus_tag="VII50_07220"
     CDS             5809..>6066
                     /locus_tag="VII50_07220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5050676.1"
                     /translation="MAYVEHYITVAAGTTLQPTPLVPEVSLYCAQNAYEVWSLTDEGD
                     ESAPMPFWSFPWAGGQALARYILDHPDVVAGRRVLDLAAGSG"
BASE COUNT         1083 a         2041 c         1902 g         1040 t
ORIGIN      
        1 tgagtagcca gcgctatgcc tgcctgatgc cgagcgtgtt cccgtcggga tcatggaacc
       61 aggcgccgcg tgccggaccg agttgtgcaa tgtgacccgt ggttgcaagc ggtccttcgg
      121 tgtagtcgag gaactcgacg ccgccggctt cgaggttctt gacctcggct tcgatgtcgt
      181 cgacttcgaa gtgggcgagg gtgtgctcgg tggcgaccgg cggccgcttg aagatcgaga
      241 gttcgctcgc gggcccgcag cgatacctgg ccgagaaggg gttctcccac aggaacgtca
      301 ggcccacggt ctcaccgtaa aacttcttgg agcgatcgag atccgtcacg ggaatcgtcg
      361 ccacgaccat cgttgcggtc atcatcagaa cgctcctccc gacatcacct tgtagacgtc
      421 gatggtggtc tcttgcggcg gagacgtgaa cccaccgtcg atgccctgct gcatacgcgg
      481 cagcaagatc gtgtcacgaa actcctccca gctccccagc gtctcgtgca cggccaggat
      541 cgtccaacct cccggtgatg cgcctgccgc gtgaaacagc tgacccgcag gcaactgtcc
      601 gtctgccggg tggacggcgg cgatcgatgc ctcgtactgc tcagcggtcc ctgctgggaa
      661 gaaatgcacg accccgtagg gcttggtgct catgtgcgct cccttggttg aaggtacttg
      721 acatgccaag tagtagacct cattacttgg catgtcaagc atttgtcgta cactcgtttg
      781 cgtgggtacc gatagagcag acagcgtcga gcgctggacg ccggcgtggc tgtcgctcgt
      841 ccgaacgcac gctcggctgt gggaccaggt cgaggcgcgc atgcgccgtg acagcgggct
      901 gacgatgccg cgctacgacg ttctcatgca tctcgaaatg gcgggtgggc ggctcgggct
      961 caccgacttg gccgcagcga tcgtgttgtc tccgtctggt ctgtcgaaac ttctcgatcg
     1021 gatggaagtc tccggcctcc tgcgtcgcga agccgatcca cacgatgctc ggtcgacatt
     1081 tgcgacgatc actccccgcg gcaagacagt ggtgaaaaag gcccggcaga gccaccacgc
     1141 gtggctacag cagctattcg gcgatgccct tgacaaccga gatcttgccg acctcactcg
     1201 tgtcatggat cggatcgaca tgcgagcgcc gcgtatatag gccgtggcac cacgccgaca
     1261 agggtcagac cgtgaagaca accttgccga acacgtcacc tgccaccatc gcggcgaacc
     1321 cttcgggtgc ctgagccagc ggcaagatcc gatcgatcgt cggccgaacg ccagtaacca
     1381 taaggaaatt cagcaactcc acgagttcct gacgggtgcc catggtcgat ccgatcaccc
     1441 gcagttgctg gaaaaacacc cgactgagct cggcggacgg tgcgtcgccg ctcgtcgcgc
     1501 cgcacgtcac gatcgtcccg ccgggcttaa ggcatcgcag cgaatgtgcc cacgtcgccg
     1561 cgccgacgct gtcgaacacg gcgtcaacgc gttccggcaa ccggccgccc gactcgtacg
     1621 ctgcgtccgc gccgatcttg agcgcaaggc cgccgcgatc cagcgatcgg ctggtcaccc
     1681 agacgcggcg tccggctgcc ttggccaagg cgatcagggc agtcgcgaca ccgccgcccg
     1741 cgccctgaac aagcacggtg tccccgggac gggtgcccga gttggtaaag agcatccggt
     1801 aggcggtcag ccatgcagtc ggcaggcagg cggcctcctc gaagctgagc ccggccggct
     1861 tgggtaggag attgcgggcc ggcacgacaa cgaattccgc aagggcacca tcgaatcgct
     1921 cggagagcag gctgcgattc gggtccatgc tctcgtcccc gcggccggcg tccggatcgc
     1981 cgatgacgcc gtggacgacg acctcgttgc cgtcgtcatc gaatcccgcg gcgtcgcagc
     2041 cgaggactct gggtagttca tcggcccgca ggccaacccc gcgcaggctc cacacgtcgt
     2101 gctggttcag tgcggccgcc ttaacatgca cccgtgccca gccatcaggg ggcgacggat
     2161 tctgtacctc accgacggta agaccgacga gcggatcggc agcgtcaaag gacatcacgc
     2221 tcgcggcaag catggagctg acggtacgcc gtcccgcagc gggcactcgc cgacgtcgcg
     2281 cggctcgcct ggcactttcg tgtccggatc tttaccgact gcccaggcgg caggcactta
     2341 acctgctgga aagatgcacc cagcatgatg tgcgtaggcc gggcgacccc cggcttgaca
     2401 tcgccggaac gcgggctcgc attgagagcc ggaaagccgg ccactacctg gaggatctgt
     2461 tgacaccgct tcgccttcga ccacgaaccc aagcagccgg catcgtcgcg atcgcgacga
     2521 tgctcgctct gaccggttgc tcgagcaaga agagcacggc agcgtccagc ccgagctcgg
     2581 cggcgccatc cgccgccagc gcggccccgt cgaccgcagc tgccccgtct gcggctgcaa
     2641 gcagcgcggc tgacccgacg tcgctgctcc ctgcggcgat caaatccgcc ggcgtgctca
     2701 atatcgcgac ggacgcgtcg tacgccccga acgagtactt cgacaccgac ggcaagacca
     2761 tcatcggtct tgacgtcgac ctcggaaacg cgatcgccgc gaagctcggc ctcaaggcca
     2821 acttccagaa cgtgaccttc aacgcgatca tccccgcgct cgccggcggc aagtacgacc
     2881 tgagcctctc gtcgttcacc gacaccaagg ctcgcgaagc gaccgtcgac ttcgtcgact
     2941 acttcaatgc cggcgagtcg ttgatggtgc tcgccggtaa cccgaagggc gtgaccggcc
     3001 ccgacctcag cctgtgcggc ttgacgatcg ccgtcgagaa gggcaccacc gaggagagcg
     3061 agatcccggc gctgtcgaag aagtgcacgg acgccgggaa gaaggccatc accggtctgt
     3121 cgtccgacga ccagaacggc gccaacctcg cgctgaccag tggccgagcg gacgtcggct
     3181 cggccgactc gccgatcgcg gcgtacgccg tcaagcaact tgcgggcaag atcgcgctgg
     3241 ctggtcagac ctacgagacc gccccttacg gcatcgcgat cccgaagacc cccgggctcg
     3301 cacccgccat caaggctgca ctcgccgcga tgcttgcgga cggcagctac aagacgatcc
     3361 tcgacaagtg gggtgacgac gccggcgcga tcaccacgcc agtgatcaac ggcgcgacca
     3421 gttgaccctg cgatgacatc aggtggcgac gtctccgtga cacctgacaa tgtcgtgagg
     3481 ggacggcccg agaacattcg ggccgtcccc gttcggcacc cgtggcgttg ggtcgcgatc
     3541 gccgtgattg ccgtgctcgc cgcgatgctc gtgcacatgc tgttcacgaa cccgaatctc
     3601 gaatggtctt ccgttcgcaa gtacctcttt agcaacatca tcctgcgcgg cgtgtggatg
     3661 accatcaagc tcaccatctt gtcgatggcg atcgggatcg tgctgggagt cgtggctgcg
     3721 gtcatgcgac tctccgagaa ccctgtggtc cgaggcgccg cgtggttgta cgtctggttc
     3781 tttcgcggca ctccgttgct cgtacagatc atcgggtggt tctcgattag cctgctcttc
     3841 aagacgctgg cgttcgggat tcccttcgga ccacattttg ccggcgagaa caccaacaag
     3901 ttggtctcac ttttcgtgag cgcgttgttg gcgttgagcc tgaacgaggg cgcctacatg
     3961 tcggagattg tccgagccgg catcctggcc gtcgacgaag ggcagaccga ggcggcgcac
     4021 gcccttggca tgacgaagct gcagaccatg cgccggatcg tgctgccgca ggccatgcgc
     4081 gtgatcgtgc cgccaaccgg caacgagacg aacggcatgc tcaagaacac tgcgctggcc
     4141 agcaccgtcg gtatcttcga gttgctgcga tcgagcgagg cgatctacaa cgccaattac
     4201 aagacgatcg gcctgcttat cgtcgccgtc atttggtacc tcgcgatgac ctcgattctg
     4261 caggtagggc agttctatct cgagcgctac tacgggcgcg gatcaactcg aacggtcgcc
     4321 ctcacgccga tccaacgggc gaggttggcg ctcatgggca accatggccg tggaggggca
     4381 aattgaccgg actcatggtc gacgcgcagc aggtgcacaa gtcgttcggg cccatcgagg
     4441 tgctcaaggg catcgacttc catgtcgctc ccggtcaggt cgtctgcttg ctcggaccct
     4501 ccggctcggg caagagcacc ttccttcggt gcatcaacca cctcgagaag atcaacgccg
     4561 gtcggctctt cgtcgatggc gaactcgtcg gctaccgcca gcacggcgac aagcttcacg
     4621 agctctcagc gaaggaggtc gccaagaagc gcaccgagat cggcatggtg ttccagcact
     4681 tcaatctctt tccgcacatg acggcgctcg gcaacatcat ggaggcaccc tgccaggtca
     4741 agaacgagcc aaaggccgag gtgcggacac gcgcaatcga gctgctggcc aaggtcggcc
     4801 tcggcgacaa ggcgagcgcc tatccccggc aactgtccgg cggccagcag cagcgggtgg
     4861 cgatcgcccg ggcgctcgcc atgcggccca agctcatgct gttcgacgag ccgacgtctg
     4921 cccttgaccc cgaactggtc ggcgacgtgc tcgccgtgat gaaggacctg gctagctccg
     4981 gcatgaccat gatcgtcgtc acccacgaga tcggtttcgc tcgcgaggtc gcggacaccg
     5041 tggtcttcat ggacggcggc gtggtggtcg agcagggacc gccgtccgag gtgatctcca
     5101 acccgcgcca cgaacgaacg aaggcgttcc tctcacaggt tctgtgacgc tcccactagg
     5161 ctcaccgtcc atggaagttc gcgaactcgc cgtccccggc gcctgggaat tcacacctcg
     5221 ccagatcggc gacgagcgtg gcgtgttcct cgagtggttc cgtgccgacg tggtcaaccg
     5281 caccatcggg cacagcctcg agatcgcgca gtgcaaccag tcggtctcgc gccgcggcac
     5341 agttcgcggc atccactatg ccgacgtgcc gcccagccag gcgaagtacg tctactgccc
     5401 gggtggcgca gttctcgacg tcatcgtcga tatcagagcc ggctccccga ccttcggcgc
     5461 cgtcgacacg gtccgtctcg acaacgtcga ccgccgcgcg gtctacctgt ccgagggcct
     5521 cgggcacgca ttcatggcgc tcacggacgg tgcgacggtc acgtatctct gctcgaccgg
     5581 ctacgcgccc gatcgcgaac acggagtgaa tccgctcgac ccggcattgg ccattccgtg
     5641 gcccgacaat ctcgagccgg tgctctcgga caaggaccgc gacgcgccca gcctggcaga
     5701 ggcggccgaa caaggtgtgc tgccgacgta cgcggagtgt ctcgcctact acgcctcgct
     5761 gcaccgcgcg cgcggctgat ccacaccggg gccggtcagg gctcaggcgt ggcgtacgtg
     5821 gagcactaca tcaccgtggc cgcaggcacg acgctccagc ccaccccgct ggtgccggag
     5881 gtgtcgctgt actgcgcgca gaacgcctac gaggtctgga gcctcaccga tgaaggcgac
     5941 gagtccgcgc cgatgccgtt ctggtcgttc ccgtgggccg gcgggcaggc gctggcgcgc
     6001 tacatcctcg accaccccga tgtcgtcgct ggacgccgcg tactcgacct cgccgccggc
     6061 tcgggg
//
LOCUS       DATCBJ010000362         2489 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_468052, whole genome shotgun sequence.
ACCESSION   DATCBJ010000362 DATCBJ010000000
VERSION     DATCBJ010000362.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2489)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2489)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2489
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_468052"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..360)
                     /locus_tag="VII50_07225"
     CDS             complement(<1..360)
                     /locus_tag="VII50_07225"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEY5050677.1"
                     /translation="MPPGDDGARNEIVALGSWDEVSMAKALVTGASGGIGSALAAELV
                     SAGYKVTALVRDRHAAQAVSGTDVATIVADLAEVDLLPAAVADCGSFDALIHCAGVSM
                     VAAVAETDPAMWQHMLSV"
     gene            579..1328
                     /locus_tag="VII50_07230"
     CDS             579..1328
                     /locus_tag="VII50_07230"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF020034.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY5050678.1"
                     /translation="MSTSHEITDAPATWAESVASDALVVWAAGEQRKGVRVWASGGAV
                     AVAAPGLARRDRLAVGGDPASAVGLVSDVLDVLPTSFRPIGDAALVRRLVRELPNLEM
                     VGEFGWMDVVHGAFRDAPTDARWLGYAELPAVTQLLAIASPTAYAQPKLPGVQRWAGV
                     EGQDGTLRSVAADAWSCAQVGFVSSLATHPDHRRRGAARAVTSFVVRELLIDHGKVAL
                     FVDSENHPARTLYESVGMEWRAVATARVRAF"
     gene            complement(1527..1880)
                     /locus_tag="VII50_07235"
     CDS             complement(1527..1880)
                     /locus_tag="VII50_07235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5050679.1"
                     /translation="MDGEWLDDAFGPDGVRVTFCFTTSVSRYVQVLNGIGLSGSILTG
                     EIRVGRSPWRRLRPQLLRALQKVVAGYVLAIEVDGGKPVYLRRQTHDAIVELADGARA
                     AVLAGGVAALASFRS"
     gene            2106..2315
                     /locus_tag="VII50_07240"
     CDS             2106..2315
                     /locus_tag="VII50_07240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5050680.1"
                     /translation="MGLFDAALDAMFGRGDSFGRCAAPGCRRRGFYRKVVGWHVEMFG
                     KKYKPNDAIILCRRHRRPAATPYNP"
BASE COUNT          439 a          841 c          780 g          429 t
ORIGIN      
        1 cacgctcaac atgtgctgcc acatcgccgg atcggtctcc gctaccgccg ccaccataga
       61 aaccccggcg cagtggatca gcgcgtcaaa cgacccgcaa tcggcgaccg cggccggcag
      121 aaggtccacc tcagcaagat ccgcgacgat cgtcgcgaca tcggtgccac tcaccgcctg
      181 ggcggcgtgg cgatcgcgaa caagggccgt caccttgtat ccggcggaca cgagttcggc
      241 tgccagcgcc gagccgatcc cgccggatgc gcccgtcacc aacgccttcg ccatgctcac
      301 ctcgtcccac gaaccgagtg cgactatctc attgcgagca ccatcgtcgc cgggcggcat
      361 cgcaaatgcg atacgcccgc cacggactcg cccgatcaga tccgcttcat cgcgatgcat
      421 cccgaccgca cgcagtcagc caggcgatcg gccacgcgct caccacgccc gacgcgaccc
      481 actcctagca cctgcgtgtc gtcacgaacg gccccgacag cggcgttggc acccatccgc
      541 tgacgtgttt tcgacgagtt ggctgctaga acatcttcat gtcgacgtcg cacgagatca
      601 ccgatgcccc ggccacgtgg gccgaaagtg ttgccagcga tgcgcttgtc gtatgggccg
      661 caggcgagca gcgcaagggc gtgcgggtgt gggcgtcagg cggcgcggtg gcggtcgccg
      721 caccgggctt ggcacggcgg gatcgtctcg cggttggggg cgacccggcg tcggcagttg
      781 gccttgtgag cgacgtgctt gacgtgttgc caacgagctt tcgcccgatt ggcgacgcgg
      841 cgctagttcg acggttggtc agggagctgc caaatctgga gatggtcggc gagttcggat
      901 ggatggacgt cgttcacggc gccttccgag acgcacccac cgacgcccgg tggctcggct
      961 atgcggaatt gcccgcggtc acgcagcttc tcgcgattgc atcgccaacc gcctacgctc
     1021 agcccaagct ccccggcgtt cagcggtggg cgggcgtcga agggcaagac ggaacgctgc
     1081 gatcggtggc agccgacgcc tggtcatgcg cccaggtcgg cttcgtgtcg agtctggcga
     1141 cacaccctga ccaccgacgg cgcggtgcgg cgcgcgccgt gacgtcgttc gtggttcgcg
     1201 aactgctcat cgaccacggc aaagtggcac tgtttgtcga ctccgagaat caccccgcaa
     1261 gaacactgta cgaatccgtc ggtatggagt ggagagccgt cgccactgcg cgcgtccgcg
     1321 cattctgacc tgccgctccc cgccgggccg aattcgccac gcgaccctcc gcacaaggtt
     1381 tctagccgtg ctcgctttct gatcacaccc aactcggcgc gatcacatga gcgtcacagc
     1441 ggcaagacag tgcgtttctg caagaccatc aggctgccta aacgcctctg tagacgatct
     1501 gccgttcgcg ctcacccgtt cgtcatctac gaccggaagc tcgcgagggc cgcaacgccg
     1561 cccgctagga cggccgcgcg agcaccatcc gcgagctcga cgatcgcgtc gtgtgtctgt
     1621 cgacgcaggt agaccggttt accgccgtct acttcgatgg ccaggacata tccggccacc
     1681 accttttgga gtgcgcggag aagttgcgga cgtagccggc gccagggcga ccgtcctaca
     1741 cgaatctcac ccgtgaggat cgatcctgag agcccgatgc cgttcaacac ctgcacgtag
     1801 cgggacacag acgtggtgaa gcagaaagtc acgcgcaccc cgtccggtcc aaacgcgtca
     1861 tcgagccatt cgccgtccac atgcccatcc tcccgcgcgg cggttcgaac tgcgtcatcg
     1921 cagcaaaatg tacgcgttag ctgaacgcgc acggcgaaag cgcgttcacg tgaacgcgct
     1981 tctgaggtat ggcgccagac acaaccatcg accgtcaaag cggcaaagtc ggatgttgga
     2041 cgctcctgtc atcgcgcttc gtggatcatg gcttgcagcc ttgatcgcct gaaaggatgc
     2101 gccccgtggg cctcttcgac gctgcgctcg acgccatgtt cggtcggggc gactcgttcg
     2161 gtcggtgcgc agcacctggc tgtcgtcgtc ggggtttcta ccgcaaggtc gtgggctggc
     2221 acgtcgaaat gttcggtaag aagtacaagc cgaacgacgc gatcatcctg tgccgtcgcc
     2281 atcgcaggcc agccgcgact ccgtacaacc cttagatgca acctggccgc ccggccgcga
     2341 tattgcgacc gtcaacgcgg cagatgatcg cgttggcgtg aacgcgcacg gcggcggaag
     2401 agtgcgtttc attgagttcg actcggtctg cgttaggtgg agtcgccggg ccggtcggac
     2461 ggcagttcgg ggaacaggcc tacgtctgc
//
LOCUS       DATCBJ010000363         2310 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_295581, whole genome shotgun sequence.
ACCESSION   DATCBJ010000363 DATCBJ010000000
VERSION     DATCBJ010000363.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2310)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2310)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2310
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_295581"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1205)
                     /gene="glp"
                     /locus_tag="VII50_07245"
     CDS             complement(<1..1205)
                     /gene="glp"
                     /locus_tag="VII50_07245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990586 - divisome complex [Evidence
                     IEA];
                     GO_function: GO:0061599 - molybdopterin molybdotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gephyrin-like molybdotransferase Glp"
                     /protein_id="HEY5050681.1"
                     /translation="MRTVDEHLTAVLGTLDPLPPLEVNLLDGHGCALAEDIVAWRPLP
                     GFDNSSMDGYAVRSVDVASATPQTPVELPVIGDIAAGSGGIYSVNTGLAVRIMTGAPI
                     PAGADCVVPVEWTDGGVARVRIDYAPPVGLSIRREGEDVLAGETVLTKGTSLGAPQIG
                     LLAAIGRARVLIRPRPRVVIFSTGNELVDVGVEPGSAQLTESNSYALSAAAREAGAIA
                     YRVGIVADDPTKLMSLIEDQLVRADLVVTTGGVSAGAYDTVKAVLSRLGTVEFLKVAM
                     QPGMPQGFGTVGPDSTPIFTLPGNPVSAYVSFEVFVRPAIRRMLGVLPLYRPLVRARL
                     AQPITKSEGKRAYVRARLDVNDGAYVVTPVGGMGSHLVADLALANAFIVAPDHVSEIP
                     TGATVSVMML"
     gene            complement(1202..2134)
                     /gene="galU"
                     /locus_tag="VII50_07250"
     CDS             complement(1202..2134)
                     /gene="galU"
                     /locus_tag="VII50_07250"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HEY5050682.1"
                     /translation="MADQKVTKAVIPAAGLGTRFLPATKATPKEMLPVVDKPAIQYVV
                     EEAVAAGLDDILMITGRSKRALEDHFDRNYELEETLRAKGDADRLSLVQEATDLATVH
                     YVRQGEPRGLGHAVLVAAQHVGNEPFAVLLGDDLIDERDPLLSRMVEVQERLGGSVIA
                     LIEVDPSQIHLYGCAAVAATDEEDVVLVTDLVEKPKVDAPSNLAIIGRYVIAPEIFDV
                     LRETPPGAGGEIQLTDALRVLAGRSSHGGPVHGVLFRGRRYDTGDRAEYLRAVITLAV
                     GRADLGPDLLAWLRIFVKQQDALQGAASEDRTNA"
BASE COUNT          396 a          780 c          760 g          374 t
ORIGIN      
        1 agcatcatca ccgacacggt cgcgccggtc gggatctcgg agacgtgatc gggcgcgacg
       61 atgaacgcgt tggcgagcgc gaggtccgcg acgaggtgcg agcccatgcc gccgacgggc
      121 gtcacgacgt acgcgccgtc gttcacgtcg aggcgtgcgc gcacgtacgc gcgcttgcct
      181 tcgctcttgg tgatcggctg cgccaaccgc gcgcgaacga gggggcgata aagaggcagg
      241 acgccgagca tgcgccggat tgccggccgc acgaagacct cgaacgacac gtaggcgctc
      301 accggattgc cgggcagcgt gaaaatcggc gtcgaatcgg ggccgaccgt gccgaagcct
      361 tgcggcatgc ccggctgcat cgcgaccttc aagaactcga ccgtgccgag ccgcgacagc
      421 accgctttca ccgtgtcata ggcgccggca ctcacaccgc cggtggtcac cacgaggtcg
      481 gcgcgtacca gctggtcctc gatgagcgac atcaacttcg tcgggtcatc agcgacgatg
      541 ccgacccggt aggcaatagc gccggcttca cgggcggcgg cgctcagtgc atagctgttc
      601 gattcggtga gttgcgccga ccctggttcg acgccgacgt cgacgagctc attgccggtc
      661 gagaaaatca ccacccgagg gcgggggcgg atcaagactc gagcgcgtcc gatcgcagcg
      721 agcagaccga tctgcggcgc tccgagcgaa gtgcctttcg tcagcaccgt ctcgcctgcg
      781 agaacgtctt caccttcgcg ccggatgctc aatcccaccg gcggcgcgta gtcgatacga
      841 acgcgagcaa cgccgccgtc cgtccactcg acggggacca cacaatcggc cccggccgga
      901 atcggcgctc cggtcatgat gcgtaccgcg agtccggtgt tcaccgaata gatgccgccg
      961 cttccggccg cgatatcgcc gatgacgggc aactcaaccg gtgtctgcgg cgttgccgaa
     1021 gcgacgtcga ccgagcgcac cgcataaccg tccatcgacg agttgtcgaa gccgggcagc
     1081 ggccgccacg ccacgatgtc ctcggcgagc gcgcacccgt gcccgtcgag caggttcacc
     1141 tcgagcggtg gcagcgggtc aagcgtcccg agcaccgcag tcaggtgttc gtcgacggtt
     1201 ctcatgcgtt cgtgcggtcc tcggaagcgg cgccctgcaa agcgtcctgc tgcttcacga
     1261 aaatccgaag ccaggcaagc aaatcggggc caagatcggc gcgcccgacg gccagtgtga
     1321 tcaccgcgcg cagatactcg gcgcggtcac cggtgtcgta tcgccggccg cggaacaaca
     1381 caccgtgcac cggaccaccg tgggacgagc ggccggcgag cacccgcaat gcgtcggtga
     1441 gctggatctc gccaccggcc cctggaggtg tctcgcgcag cacgtcgaat atctccggcg
     1501 cgatcacgta ccggccgatg atcgcgagat tgctcggcgc gtccaccttc ggcttctcga
     1561 ccagatcggt gaccaggacg acatcttctt cgtcggtcgc cgcgacggcc gcgcagccgt
     1621 acagatggat ctgcgaaggg tcgacttcga tcagcgcgat gacgctgccg ccgagccgtt
     1681 cctgtacctc aaccatgcgc gacaacagcg ggtcgcgttc atcgatcagg tcgtcaccaa
     1741 gcagcaccgc gaacggctcg ttgccgacgt gctgcgcagc gacgagcacc gcatgaccca
     1801 acccgcgcgg ttcgccctgg cgcacgtagt gcaccgtcgc cagatccgtg gcctcttgta
     1861 cgagggacaa ccggtcggcg tcccccttgg cgcgaagcgt ctcttcgagt tcgtagttgc
     1921 ggtcgaagtg gtcctcgagc gcccgcttgc tgcgacccgt gatcatcaag atgtcgtcga
     1981 ggccggcggc aacggcctcc tcgacgacgt actggatcgc cggcttgtcg acgaccggca
     2041 gcatctcttt cggcgtcgcc ttcgtggcag gcaggaagcg ggtgccgaga ccggcggcag
     2101 ggatgacggc cttggtgacc ttctgatccg ccatgaccga accctaacgt tttgggtcgg
     2161 tcctcgggca aaccgggctc agtcgtcgct gaagcggggg cggtctgcgc cttcaggtag
     2221 ggcgatgtcg gtgtccggga cgacgagaac tcggcgttct tggttcgtgc cgtcttcctc
     2281 ggttgctaga gcaaagcgcc acccgtcgcg
//
LOCUS       DATCBJ010000364         3229 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_486076, whole genome shotgun sequence.
ACCESSION   DATCBJ010000364 DATCBJ010000000
VERSION     DATCBJ010000364.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 3229)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3229)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3229
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_486076"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1003
                     /locus_tag="VII50_07255"
     CDS             <1..1003
                     /locus_tag="VII50_07255"
                     /inference="COORDINATES: protein motif:HMM:NF013694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HEY5050683.1"
                     /translation="RLLLTATEMARTRLYRNGVLEKENFPIAEISECVNEPGAVVWLD
                     ICTLDDGAGLDEIEEEFGLHALAIEDAAHERQRPKLDHYGTHLFLSAYAVAIDSETMN
                     LESSELAMFVMPNALITVRKDRKFDIDEVLKRWDATPELVKEGAGFLLYGVLDYIVDG
                     HFQAVQDLDEKIESLQDDLFDDSRDRVREVQRKSFDLRRDLILLRRFVLPMREVVNGL
                     MRRDVKVVNDTLMPYYQDVYDHVLRATEWTESLRDLVSTVLETNLTIQGNRMNLIMKQ
                     VTSWAAIIAVPTAVTGFYGQNVPYPGFGHDSGLITSSLIIVGGSGLLYWVFRRKDWL"
     gene            complement(1028..1822)
                     /locus_tag="VII50_07260"
     CDS             complement(1028..1822)
                     /locus_tag="VII50_07260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5050684.1"
                     /translation="MSDDEAPVPAEHHHHGAGATAAALTALERRLLAQAKGVYQIEQD
                     LLPAWLRPTQGEHRWQMGVAAGTAIGLQSSLPASLSLRPHYLLPILETILLVGLIVFS
                     PGRIVRHSTPLRAAVLALIAVISIDNAISAGLLVHSIVSGTANDAGRLLLEGVAIWTT
                     NVIAFSLWYWEFDRGGPVARAHALHKHPDLLFPQMQQPDIAPPEWEPTFFDYLFTSFT
                     NATAFSPTDTMPLSHWAKATFLAQSGISLVTVALVISRAVNIFHGG"
     gene            1896..2327
                     /locus_tag="VII50_07265"
     CDS             1896..2327
                     /locus_tag="VII50_07265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5050685.1"
                     /translation="MTRLMRICASIGAVTLATACAAHGTAKSGAGVATPVGSPGPGAP
                     PISGALGVSVGDGPPGTVITLNAIRCPPQGSKPDVIFWRDSAQPDSKGGLPLTQIVRT
                     GDALQATFVLPSDAPVGLGTFETYCGKKAQGAMAGFNVDPR"
     gene            2483..3100
                     /locus_tag="VII50_07270"
     CDS             2483..3100
                     /locus_tag="VII50_07270"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEY5050686.1"
                     /translation="MAVGDSEPSDSRLWARACAGDHSAFGDLFVRHANRIHNYCFRRT
                     GSWALAEDATSQTFMEAWRKRSTIAVTDSLLPWLFVAANNVCRNTERTRRRATNLLAK
                     TPLTEHVADHADEVAARIDSERQMQRVLSALRTLKRADQDVVAMCDWEGRSYDEAAAA
                     LGVPVGTVRSRLSRARARLKVLLADEVGPPADTRVGTDELRGEQA"
BASE COUNT          597 a         1047 c         1049 g          536 t
ORIGIN      
        1 ccgacttctg ctgaccgcaa ctgagatggc gcgcacgcgg ctgtaccgca acggggtgct
       61 ggagaaagag aacttcccga tcgccgagat ctccgagtgc gtcaacgagc cgggcgccgt
      121 cgtatggctc gacatctgca cgctcgacga cggggcaggc ctcgacgaga tcgaggaaga
      181 gttcggcctg catgccctcg ccatcgaaga cgccgcgcat gaacggcaac gacccaagct
      241 cgaccactac ggcacccact tgttcttgag cgcctacgcc gtcgccatcg actcggagac
      301 gatgaacctg gagagcagcg agctcgcgat gttcgtcatg ccgaacgcac tgatcacggt
      361 gcgcaaggat cgcaagttcg acatcgatga ggtactcaag cgctgggatg caacgcccga
      421 gctagtgaag gaaggtgccg ggttcctgct ctacggcgtc ctcgactaca tcgtcgacgg
      481 gcacttccag gccgtgcagg atctcgacga aaagatcgag tcgctacagg acgacctctt
      541 cgatgacagc cgcgaccggg tgcgcgaggt acagcgcaag tcgttcgacc tgcgacgcga
      601 cctgatcctg ctgcgacgtt tcgtgcttcc gatgcgcgaa gtggtgaacg ggctgatgcg
      661 gcgcgatgtc aaggtcgtca acgacacgtt gatgccgtat taccaggacg tgtacgacca
      721 cgtgctgcgc gcaacggagt ggaccgaatc gctacgcgac ctcgtgtcaa cggtgctgga
      781 aacgaacctg acgatccagg gcaacaggat gaacctcatc atgaagcagg tcaccagctg
      841 ggccgcgatc atcgcggtgc ccacagcggt gaccggcttc tacggccaga acgtcccgta
      901 tccgggcttc ggccacgaca gcggcctcat cacgtcatcg ctgatcatcg tgggcggctc
      961 gggcctgctg tattgggtct tccgtcgcaa ggactggctg tagcgctcag gctgtagaac
     1021 gagaaggtca gccgccatgg aagatgttga cggcgcgcga aattacgagg gcgacagtca
     1081 cgagcgagat ccctgattgc gcgagaaacg tcgccttcgc ccagtgtgaa agcggcatcg
     1141 tgtcggtcgg gctgaacgcc gtcgcgttgg tgaacgaagt gaacaggtag tcgaagaacg
     1201 tcggctccca ttcgggaggc gctatgtccg gctgctgcat ctgcgggaac agcaaatcgg
     1261 gatgcttgtg caacgcgtgt gcccgcgcga ccggcccgcc acgatcgaac tcccagtacc
     1321 aaagactgaa ggcgatcacg ttcgtcgtcc agatggcaac accctcgagc aacagcctgc
     1381 ccgcgtcatt ggccgttccg ctgacgatgg agtgcaccag aagtccggcg gatatcgcgt
     1441 tgtcgatgct gatgaccgcg atcagtgcca gcaccgccgc tcgcaggggc gtggagtggc
     1501 ggacgattcg gccagggctg aacacgatga gtccgacgag caggatcgtc tcgagaatcg
     1561 gcagcaggta gtgcggccgc agcgacaagg atgcgggcag cgacgactgc agtccaatgg
     1621 ccgtgccggc cgcgacaccc atctgccatc ggtgctcgcc ctgcgtcggc cgcagccacg
     1681 ccggcagcag atcctgctcg atctgataga cgcccttggc ttgcgcgagc agccggcgct
     1741 cgagcgcggt gagcgcggct gccgtggccc cggcgccgtg atggtggtgc tctgctggca
     1801 ccggagcttc gtcgtccgac atcttcgtcc tctcgagccc ggctaccgtg tcccagtctg
     1861 ccgctccgag gtggcgctcg cctacgatct gcgccgtgac acgtctgatg cggatctgtg
     1921 cgtcgatcgg ggccgtgacg ctggccacag cttgcgcggc tcatggcacc gccaagtcag
     1981 gcgccggggt ggcgaccccc gtcggatcgc ctggtccggg cgcaccgccg atcagcggcg
     2041 ctctcggtgt ctccgtcggc gacgggccgc ccggaaccgt catcaccctg aacgcgatcc
     2101 ggtgccctcc gcaggggtct aagccggacg tgatcttttg gcgcgacagc gcgcaaccag
     2161 acagcaaggg cggcctgccg ctgacccaaa tcgtccggac cggcgatgca ctgcaggcga
     2221 cctttgtcct gccgagcgat gcgcccgtgg gtctgggaac cttcgagacc tactgcggca
     2281 aaaaggcgca aggggccatg gcagggttca acgtcgatcc ccgctgaatt gggtatttgc
     2341 gccctcaacc attgagccca cgggtatcac cggttaccat ctgcgtggcg tctggctctc
     2401 ctcccccgta gggtcagacg cctttaatgt gcccaggttg gcgatgatgg aactcacatc
     2461 aggctgccgg acataggagt acatggcggt cggcgacagc gaaccctcgg actcgcgctt
     2521 gtgggcgcgc gcctgcgccg gcgaccattc cgcattcggc gacctgttcg tccggcacgc
     2581 gaatcgcatc cacaactact gctttcggcg caccgggtcg tgggcgctcg cggaggacgc
     2641 cacctcccag accttcatgg aggcgtggcg caagcggtcc acgatcgctg tcaccgactc
     2701 gctgctgccc tggctgtttg tggccgcgaa caatgtctgc cgcaacacag agcgcacccg
     2761 ccgccgagcg acgaacctgc tcgcaaagac accgctgacc gaacatgtcg cagatcacgc
     2821 cgacgaggtc gccgcccgga tcgacagcga gcgccagatg caacgcgtgc tgtcagcact
     2881 acgcaccctc aagcgggccg accaggacgt cgtcgcgatg tgtgactggg aaggccgcag
     2941 ctacgacgag gccgcggccg ctctcggcgt gcccgtaggc accgttcggt cacggctctc
     3001 gcgagcccgc gcgcggctca aagtactgct cgccgacgag gtcggcccgc ctgccgacac
     3061 ccgtgtgggc acggacgagt tacggggtga acaggcatga gtgttcagta cttgccacag
     3121 acacgggacc taccgcctga gcagatcctg gcgatgcgcg ccgagttgga agcgttcgtt
     3181 gctcgcgagg aaccggcgac accgtggcgg aagcggcgca acatcaccg
//
LOCUS       DATCBJ010000365         2063 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_174314, whole genome shotgun sequence.
ACCESSION   DATCBJ010000365 DATCBJ010000000
VERSION     DATCBJ010000365.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2063)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2063)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2063
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_174314"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            89..865
                     /locus_tag="VII50_07275"
     CDS             89..865
                     /locus_tag="VII50_07275"
                     /inference="COORDINATES: protein motif:HMM:NF024743.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HEY5050687.1"
                     /translation="MNATGGRVAALTVGLPFVVAAAVYGAFTMVGTFAHASERHVVSY
                     PWKGGAITLRTAGDVTVEVGSDANVGVTYSERYQLKRPTVTSSESASGLQLTATCPGW
                     HFGSNCAVNYVLTVPVTAALNIRSGDGDIHLSGTSAAATLDSGAGGIEFDNVTGNVAA
                     QSGDGDISGSQVGSTNVQVSTGAGDVNIDWSVAPTTVVATSGDGGIHLVLPQSSGPYR
                     TSTHAGNGSVHVSSLVDSTAAASITATTGTGDISIGYATP"
     gene            complement(889..1353)
                     /locus_tag="VII50_07280"
     CDS             complement(889..1353)
                     /locus_tag="VII50_07280"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY5050688.1"
                     /translation="MAVTLQPMAPEDIAPWLEQSTADHIADRVDTGEDPETVRRSTHA
                     TSETLFPGGVPAEGQFVFVVTEDAIPVGSVWLGAGRDGPPNVWFLWSIEIDVAARGHG
                     LGRQAVRLAEDFARKRGAAKLELNVFGNNSVARGLYEALGYRISTIQMSKVL"
     gene            complement(1376..>2063)
                     /locus_tag="VII50_07285"
     CDS             complement(1376..>2063)
                     /locus_tag="VII50_07285"
                     /inference="COORDINATES: protein motif:HMM:TIGR00374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="flippase-like domain-containing protein"
                     /protein_id="HEY5050689.1"
                     /translation="TPAGLGGAAVNARYLTRRGIAAGRAAAAVGVCGLAHIVVAAIGF
                     AVFGPGVAMRAAGWFFGGLSTVDMLWVGAALLVAVIARAVNLRTSRATSVLSKSWVGR
                     TAADARTAAADLVGQPARLAVLITCTAAVKASNLLALYAALWAFDGDVATWRVAVVYL
                     VGATAAEIVPTPAGLGTVDAALIAGLVTTGVTGGATLAAVIVYRLLSFWAPILPGLIA
                     SAVLRRRLAL"
BASE COUNT          358 a          688 c          697 g          320 t
ORIGIN      
        1 tcaccaagct cgatctgctg ccggctgaca ccgaccatcg ccgagtgctc gccgtcctgc
       61 gttatctgga gagttgaggg cgactgtcat gaatgcaacc ggtgggcgag tggcggcatt
      121 gacggtgggt ctgccctttg tcgtcgcggc cgctgtctat ggcgcgttca ccatggtcgg
      181 taccttcgcg cacgcgagcg aacgccacgt agtgagctat ccgtggaaag gtggcgcgat
      241 cacgctgcgc acggccggcg acgtcacagt cgaggttggc agcgacgcga acgtcggcgt
      301 cacctattca gagcgctacc agttgaagag accaaccgtg acgtcgtcgg aatcggccag
      361 cggccttcag ctgacggcca cctgtccggg ctggcacttc ggcagcaact gcgcggtcaa
      421 ttacgtgctc accgtgccgg tgacggcggc gctgaacatc cgcagcggcg acggcgacat
      481 tcatctgagc ggaacctcgg cggcggccac cctcgatagc ggcgccggtg gcatcgagtt
      541 cgacaacgtg accggcaacg tggcggccca aagtggcgac ggcgacatca gcggcagtca
      601 ggtgggttcg acgaatgttc aggtatcgac cggcgctggc gatgtgaaca tcgactggtc
      661 agtagcgcca acgacggttg ttgcgacctc tggcgacggc ggtattcacc tcgtgcttcc
      721 acaaagcagc gggccgtatc gaacctcgac ccacgcgggc aacgggagcg ttcatgtcag
      781 ttcgctggtc gactcgaccg ccgccgcctc gatcaccgca acaacgggca ccggtgacat
      841 ctcgatcggg tacgccactc cttgattcat ggccaagggc aggtctagtc acagcacctt
      901 ggacatctgg atggtcgaga tccggtagcc gagcgcctcg tacagaccac gggccaccga
      961 gttgttgccg aacacgttca gctcaagctt ggcggcaccg cgtttgcgcg cgaagtcctc
     1021 ggcgagcctg accgcctggc ggccaagccc atggccgcgg gccgcgacgt cgatctcgat
     1081 cgaccacaga aaccagacgt tgggcgggcc gtctcgtccg gcaccgagcc acacgctgcc
     1141 aacggggatc gcgtcctcgg tgaccacgaa cacgaactgc ccttcggcgg gcacaccgcc
     1201 tggaaagagg gtttcgcttg ttgcgtgggt gctgcgtcgg acggtctcgg ggtcctcacc
     1261 cgtatcgact cggtccgcga tgtggtcggc cgtgctttgc tcgagccacg gggcgatgtc
     1321 ttccggcgcc atcggctgca gggtgaccgc cataccactc agggtagccg tctggttaga
     1381 gcgcgaggcg gcgccgcagc acggccgagg cgattaagcc agggaggatc ggtgcccaga
     1441 acgacagcag ccggtagacg atgacggcgg caagtgtcgc tccgccagtc accccggtgg
     1501 tcaccagacc cgcgatcagg gcggcatcga cagtcccgag gccggccggc gtcggcacga
     1561 tctcggcggc cgttgccccg acgaggtaga ccaccgcgac acgccaggtg gcgacgtccc
     1621 cgtcgaatgc ccacagcgcc gcgtacaggg cgagcagatt cgacgctttc accgcggccg
     1681 tgcaggtgat cagcaccgcg agtcgcgcag gctgcccgac gaggtcggcg gctgcggttc
     1741 gagcatcggc cgcggtgcgt ccgacccagc tcttgctgag cacggacgtc gcgcggctgg
     1801 tgcgcaggtt gacggcccgc gcgatgacgg cgacgagaag tgcggcgccc acccagagca
     1861 tgtcgacggt gcttagccca ccgaagaacc agcctgccgc gcgcatcgcg acgccaggac
     1921 cgaagaccgc aaagccgatg gccgcgacga cgatgtgcgc gagcccgcag accccgaccg
     1981 ctgctgccgc tcgtccggca gcgatacccc gacgcgtcaa gtagcgcgcg ttcactgccg
     2041 ctccgccgag cccggcgggg gtg
//
LOCUS       DATCBJ010000366         2083 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_470701, whole genome shotgun sequence.
ACCESSION   DATCBJ010000366 DATCBJ010000000
VERSION     DATCBJ010000366.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2083)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2083)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2083
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_470701"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1037
                     /locus_tag="VII50_07290"
     CDS             <1..1037
                     /locus_tag="VII50_07290"
                     /inference="COORDINATES: protein motif:HMM:NF033542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HEY5050690.1"
                     /translation="RLLADGELVVDVPPKLSARLRVFTTGQGRKTDATDAHSVALVGT
                     RMSGLRPVVNDEQLTVLRLLVDRRRSLGDDHVRMIAQLHRVLLELIPGGAKRYLSAAQ
                     AKALLGKVRPRDTAGKTRRRVAAELIADLERIYERTKAADKELKELLAATGTTLTQLH
                     GIGPSGAARLLVEVGDITRFPSRAHFASWTGTAPIDASSGDHVRHRLSRGGNRQINRV
                     LHIMAVVQLRSQTEGRAYYDRKKAAGKTSMEAMRCLKRRLSDVVYRQFLADAIRPSKA
                     GPGGHSGAALDSSAVDSNPDIDASKKSLPGPANTHATRAKSSRPDRSSARSRRPVTAP
                     ARSRRQAQSA"
     gene            complement(1121..1303)
                     /locus_tag="VII50_07295"
     CDS             complement(1121..1303)
                     /locus_tag="VII50_07295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5050691.1"
                     /translation="MTIRKDPLFRVVAYALLLGAVVSGALGQILLAALLGAAAFLCLL
                     VRLTFAIQRGRARRTT"
     gene            1458..1754
                     /locus_tag="VII50_07300"
     CDS             1458..1754
                     /locus_tag="VII50_07300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5050692.1"
                     /translation="MSKRQALALATILALCAVVVFAAHGLWRRHESAALARTPSYKYG
                     ASVMDLVTKDLLVRVFDPRGACRNDLASMPGSFSGYSASVAIKGCVDEWTHLNG"
     gene            complement(1846..>2083)
                     /locus_tag="VII50_07305"
     CDS             complement(1846..>2083)
                     /locus_tag="VII50_07305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5050693.1"
                     /translation="SRHQSPGQPPSTTAAPDDIGFSLPAGQESGTGALLFNATGCLNW
                     TLLAYDASNKLVAQLQAPICKVHGHVPTWTITTK"
BASE COUNT          388 a          676 c          659 g          360 t
ORIGIN      
        1 aacggctact cgccgacggt gagctggtgg tcgacgtgcc accgaaactg tccgcccggc
       61 tacgggtgtt caccaccggc cagggccgaa agaccgatgc caccgacgca cattcagtcg
      121 cactggtagg cacccggatg agtggcctgc ggccggtggt caacgacgag cagctgacgg
      181 tgttgcggct gctggtcgat cgtcgacgtt cccttggtga tgaccatgtg cggatgatcg
      241 cgcagctgca ccgggtactg ctcgagctga tccctggtgg ggcgaagagg tacctatccg
      301 cggcgcaagc caaggcattg ctggggaaag tccgaccgcg cgatacggcc ggcaagaccc
      361 gacgccgtgt cgccgctgag ctgatcgccg atctcgagcg gatctatgaa cgcacgaaag
      421 ccgccgacaa agaactcaaa gaactgctcg cggcaacggg cacgacgttg acgcagttgc
      481 acggcatcgg gccttccggt gccgctcggt tgctcgtcga agtcggtgac atcacccggt
      541 tccctagccg tgcgcacttc gcgtcgtgga ccggcaccgc accgatcgac gcatcgtctg
      601 gtgaccacgt gcggcaccgc ctgtcgcgtg gcgggaaccg gcagatcaac cgcgtgctgc
      661 acatcatggc cgtcgtccag ttgcgcagcc aaacagaagg gcgcgcgtat tacgaccgga
      721 agaaagccgc aggcaagacg tcgatggaag cgatgcgctg cctcaagagg cgactgtccg
      781 acgtcgtcta ccggcagttc ctcgcggacg cgatccggcc gagtaaggca ggcccgggag
      841 gacactcggg ggcggctctt gactccagcg cggtcgactc aaaccccgat atcgacgctt
      901 cgaagaagtc acttcccgga cccgccaaca cccacgctac ccgcgcgaaa agctcccggc
      961 cagaccgttc ttcggcccgt tcccgacgcc ctgttacagc gccggcccgc agccgtcgtc
     1021 aagcgcagtc tgcttgacga cggcgaggac cggcgcacca tcgacaggcg ggaacaaccc
     1081 ttcattgaca tagaagggag cctaatgcgg atgtatgccg ttaggttgtg cggcgagccc
     1141 gaccgcgctg gatagcgaac gtcagcctca ctaggaggca caggaatgca gccgctccca
     1201 acaaggcagc gagcaagatc tggcctaatg cccccgagac caccgcgcca agaaggagcg
     1261 cataggccac gactctgaac agcggatcct tgcgaatggt catccagagt tcctccacat
     1321 cgctccatac cctcgcgtgg gcgaaagcca aaccgtctgc cagctcaatc ccaatcgcgc
     1381 gtcatcgcgg cagttgcgga tgcatttggg cgagcagaat ccgcgctcga cgcctcggat
     1441 cgaggcagaa tcatcgtgtg tccaaacggc aggcccttgc gttagccacg atcctggccc
     1501 tctgcgccgt cgtcgttttc gccgcgcacg gcctgtggcg tcggcacgag tccgccgcgt
     1561 tggctcggac gccgtcgtac aagtacggcg cgtccgtgat ggacctagtc acgaaggact
     1621 tgttggtccg agtgttcgat cccagggggg cgtgtcggaa cgacctcgcg tcaatgcccg
     1681 gctcgttctc ggggtattcg gcgtcggtgg cgatcaaagg gtgcgtcgac gagtggacgc
     1741 atttgaacgg gtagctcgca cgggttactc attcgtctcg cggtcggggg caacgaccca
     1801 tttcgcaccg cagtacgacc gtcaacgcgg caaatgtgtg cgttgctact tcgtggtgat
     1861 cgtccaagtc ggcacgtggc catgcacctt gcagatcggt gcctgcaact gcgccaccag
     1921 tttgttgctc gcgtcgtagg cgagcagcgt ccagttcagg catcccgtcg cgttgaacag
     1981 cagcgctccc gtcccactct cctgcccggc gggcaggctg aacccgatgt catccggagc
     2041 cgctgtggtc gacggtggct gacccggact ttggtgtctg ctc
//
LOCUS       DATCBJ010000367         2884 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_305269, whole genome shotgun sequence.
ACCESSION   DATCBJ010000367 DATCBJ010000000
VERSION     DATCBJ010000367.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2884)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2884)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2884
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_305269"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..190)
                     /locus_tag="VII50_07310"
     CDS             complement(<1..190)
                     /locus_tag="VII50_07310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5050694.1"
                     /translation="MDTPLYTEPDPASNEPSATTSTTSLRRDQVYGELRRRLMMGEFP
                     VRSRLVEERLASLLGVSRT"
     gene            304..960
                     /locus_tag="VII50_07315"
     CDS             304..960
                     /locus_tag="VII50_07315"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY5050695.1"
                     /translation="MHKARTGIVRRVVLIHGAATTSRIWDRVVPLLGGFDVHCPDRRC
                     SGNLDVEVADLMPLCKHAIVVGVSGGATLGLELAARDCGAASIIVHEPAVGSLLPGLL
                     NAVATAYASGGVPAFATTLYGPSWQPADGPADDASVARDIAMFRAFEPSAPNPEGCPV
                     TVTVGALSAQIRHAAAAALATRFGLAVRTLPDAEHAIHLDRPAVLASLIRDTATALGA
                     "
     gene            1264..2484
                     /locus_tag="VII50_07320"
     CDS             1264..2484
                     /locus_tag="VII50_07320"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HEY5050696.1"
                     /translation="MSSPAYTEALRVLSLGIDTDLNAALLAALKPMDGTDPVVYLADF
                     SRRTLIPVPTGDSRSDVGEEEVSSTMAGRAFITRKPVCAERADGFRLWVPLVEGTMRT
                     GVLAVTLPLVDDEKLAHVEMLGVFAGLALAATAPLSDLSHIRRRGRTMSLAATMQWSL
                     MPPLAAATQRAAIAGVLEPAYDIAGDGFDYAINADAIEFAILDGMGHGVASSMLTGLA
                     IGAYRHSRREGSSLPKMHDAIERAIFEQYHGEAFATGIIGRLSTATGELDWSCAGHPA
                     PLLLRERRVIAELECETVLPFGLGEEGPVSVGLQVLQPGDAVLLYTDGVVEARTTTGE
                     EFGLPRLIDLFEREAASERTGEELLRRVVRAVLDYQVDQLRDDATMMLIEWTGATERL
                     ASDIPEQRPEKALG"
BASE COUNT          468 a          966 c          953 g          497 t
ORIGIN      
        1 gcgtgcgcga caccccgagc aggctcgcga gccgctcctc gaccagccgg ctgcgcaccg
       61 ggaactcgcc catcatgagg cggcgccgca attcgccata gacctggtcg cggcgcaacg
      121 atgtggtact ggtcgtcgcc gacggttcat tcgacgccgg atccggttct gtatacagag
      181 gtgtatccat gccgcgaaaa gctaggagca gcgcattgcc gaagaatatg gagcgtgtaa
      241 cttccgcgtt gctgcgcctc gctcccatcg acgagcgcga cacatgcgtg atacacacca
      301 gccgtgcata aggcgcgcac cggcatcgtc cgccgggtcg ttctgattca cggagccgca
      361 acgacgtccc gcatctggga tcgggttgtt ccgctcctcg gcggcttcga tgtccactgc
      421 cccgaccgcc gctgctcggg caatctcgac gtcgaggtgg ccgacctgat gccgctgtgc
      481 aagcacgcga tcgtggtcgg ggtcagcggg ggagccaccc tggggcttga actggcggcg
      541 cgcgactgcg gcgccgcgtc gatcatcgtg cacgagcccg ccgtgggatc gctgctaccg
      601 ggcttgctca acgccgtcgc caccgcgtac gccagcggtg gcgtgccggc cttcgcgacg
      661 acgctgtacg ggccgagttg gcaaccagcc gatggtccgg ccgacgatgc ctccgtcgct
      721 cgagacattg caatgttccg cgctttcgaa ccatcagcgc caaaccctga gggctgcccc
      781 gtcacggtca cggtcggagc actatctgcg cagattcgcc acgccgccgc ggcggcgctg
      841 gccaccaggt tcggactcgc cgtccgcaca ctgccggacg ccgaacacgc gatccacctc
      901 gaccgaccgg ccgtgctcgc cagcctcatt cgcgacaccg cgaccgccct cggcgcctga
      961 cagcgcggaa ggggcgtgaa gattctcggc cgcgttggtt aagctccggc gctcctccac
     1021 tgcaacgaat gtacggagtt gccgcgactc gaaccccacg tcgggacgca tgccccagcc
     1081 gtagcgggag cggcgcttgt gaccaacaac agtgcgttgt cgacggataa gctttctcaa
     1141 gccgcgggtc gtcgtgggta gcggaatctc ttggccgaag cctgctcaat ctcgtccctt
     1201 ggcgcagcat ccgggctacg gttgcctcga gctgctttcg cggccgctcg gaatggagcc
     1261 cgggtgtcgt ctcccgcgta cacggaggcg ctccgcgtcc tgagccttgg tatcgacacc
     1321 gatctgaacg cggcactgct ggcggcgctc aagccgatgg acggcaccga ccccgtcgtc
     1381 taccttgccg atttctcccg tcgcacgctg ataccggtgc caaccggaga ctcgagaagc
     1441 gatgtcggcg aggaggaggt ctcgtcgact atggctggcc gcgctttcat cacccgcaag
     1501 cccgtctgcg ccgagcgcgc tgacggcttt cggttgtggg tcccgctcgt cgaaggcact
     1561 atgcgcaccg gcgtcctcgc cgtcaccctg ccactggtcg acgacgagaa gctcgcacac
     1621 gttgaaatgc tcggcgtctt tgcgggactg gcgctggctg caaccgcacc gctaagtgat
     1681 ctctcgcaca tacgccgccg cggccggacc atgtcgctcg ccgcgaccat gcagtggagc
     1741 ctgatgccgc ctctggccgc cgcgacgcag cgggcagcga tcgccggtgt cctggaacct
     1801 gcctacgaca tcgccgggga tggcttcgac tacgcgatca acgccgacgc gatcgagttc
     1861 gcgatccttg acgggatggg gcacggcgtg gcatcgagca tgctaaccgg cttggccatc
     1921 ggtgcctatc gacactcgcg gcgcgagggt tcgtcgctac cgaaaatgca tgacgcgatc
     1981 gagcgggcga tcttcgagca gtaccacggc gaagccttcg ccaccggcat catcggccgg
     2041 ctgtccaccg ctacgggcga actcgactgg tcctgcgccg gacatcctgc gccactgctg
     2101 ctgcgtgagc gccgcgtcat cgcggagctg gagtgcgaaa cggtgctgcc gttcggactc
     2161 ggcgaggagg ggccggtctc ggtcggcctg caggtgctgc agcctggcga cgcggtcctg
     2221 ctctacaccg acggggttgt agaggccagg accacgacag gtgaagagtt cggtctgcca
     2281 cgcttgatcg acctgttcga gcgtgaagca gcgtctgagc ggaccggcga ggaactgctc
     2341 cggcgggtcg ttcgggcagt gctggattat caggtggacc aactgcgcga cgacgcgacg
     2401 atgatgctca tcgaatggac gggcgcgact gaacgcctgg ccagcgacat cccggagcaa
     2461 cggccggaaa aggcactcgg gtaaagcacc ggacgatctg cgtctgcggc cggcctcgtg
     2521 tcgctcaccg cacttccaca ccgtcgccgg acacgattgt ggctcgaccc gatcgcaaca
     2581 gctgcaacag cctcttctcg gcaatgatcg tgttcgcgcg ccggcgatct tgtgctgccg
     2641 aagaccccac gcgttgccgt gctggcgcga ccggggggcg cgtgactgaa atccccggcc
     2701 ggcaggttgc gggagtgtcg cgatatggcg atctgttgat gaacgcgtca tcgccgcgat
     2761 cgtgtcagcg attggagatg gcgctgacgc caacgtgcga cgaccagcgg gggaccgggt
     2821 ggggaccaac atgttgatgt gcctgctagg gaacgcgtct gtccgcttgg acgtctgggg
     2881 tggc
//
LOCUS       DATCBJ010000368         2822 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_84733, whole genome shotgun sequence.
ACCESSION   DATCBJ010000368 DATCBJ010000000
VERSION     DATCBJ010000368.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2822)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2822)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2822
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_84733"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..619
                     /locus_tag="VII50_07325"
     CDS             <1..619
                     /locus_tag="VII50_07325"
                     /inference="COORDINATES: protein motif:HMM:NF013073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HEY5050697.1"
                     /translation="EVDSLRTKQDELTAQAANATTDAQNALAEQQAALQGANAQIVTL
                     EQQFQAQVAAEAAARDAATLAAARRAAAAVGLVDTGGVSRTALLAIQAAQSQIGKPYV
                     YGGSGPDSWDCSGLTQWAFARAGVLIPRTAADQYAAIPNKIALGQIEPGDLLFWATDI
                     SDPTTIHHVAIYVGNGLMLAAPHTGVNVQVQPVYLDGYIGAVRVG"
     gene            667..2238
                     /locus_tag="VII50_07330"
     CDS             667..2238
                     /locus_tag="VII50_07330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5050698.1"
                     /translation="MGGAPAVAPGLLVAECYRIESPLTGETETERVGTLWRAYDEVLA
                     RPVAILLARGDDPGVREVLAGARAAAGLTHAAVVRVYDAGEALGLAFVVTEFLSGGSL
                     ESQLLIGPLDPAVAVDLVSDLAIGVAAAHHVGLHGLSLTPRSVLFTSTGAPRLVGIAL
                     AGYTARSAAPPALAVSSDAEPHYAGDVGELERARMDAVGLANLLYASLTARWPGPDVD
                     SALPPAPFTEGHLRTPRQVRGGVPREVDEVVTQALGDETLLRGLPEIASPAEFAAALA
                     PLRASTDDAHPYGADTGPITELETRQSRLVNTRLVSVQVWQRWALGIGVLLLAVVVAG
                     LLLGGKHGYLSFVRGAAPAALDPTPTATPKTTSSAAAVAAAEPGAIAPGLVTEFDPYG
                     KHNDPHVSEAPRAVDGNAATMWRTQIYRTADLGKLKPGVGLLMDLGSPQKVAAVHLQL
                     VGTGTSLEVFESNSAPVNEKAMTLAVAQDGVPSDVTLRLTTTTTARYWLVWLTKLPAT
                     GTGFQGGIAEMTFLS"
     gene            2270..>2822
                     /gene="sigM"
                     /locus_tag="VII50_07335"
     CDS             2270..>2822
                     /gene="sigM"
                     /locus_tag="VII50_07335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor SigM"
                     /protein_id="HEY5050699.1"
                     /translation="MTDGVTDDPDAALLTRHLEGDPAAFTEIFQRHRDQLWAVALRTT
                     GDPEDAADAVQEALISAMRHAASFRGDAKVSTWLHRIVVNACLDRLRRRAAHPTSPLP
                     DDDHAPSTTRDDYADRDTAADVHAALATLPAEQRAAIVLVDIEGYPVDEAAAMLDVPS
                     GTVKSRCARGRAKLVPLLSHLNGG"
BASE COUNT          464 a          983 c          943 g          432 t
ORIGIN      
        1 tgaggtcgac agcttgcgca ccaagcagga tgagttgacg gcccaggccg cgaacgcgac
       61 tacggacgcg caaaacgcgc tcgccgaaca gcaggcagcg cttcagggtg cgaacgccca
      121 gattgtcacg ctggagcagc aattccaggc ccaagtcgcc gccgaggccg ccgcgcgcga
      181 cgcagcgaca ctggccgccg cccggcgcgc cgcggctgct gtcggtctgg tcgacaccgg
      241 aggcgtcagc cgcactgcgc tgctcgcgat ccaggcggcg caatcccaga tcgggaagcc
      301 ttacgtgtac ggcgggtcgg ggcccgacag ttgggattgt tcgggcctga cccaatgggc
      361 gttcgcgcgg gctggtgtgc tgattccgcg cacggcagcc gatcagtacg cggccatacc
      421 gaacaagatt gccctcggcc agatcgaacc tggcgaccta ctcttctggg cgaccgacat
      481 ttcggatccc acgaccatcc atcacgtcgc gatctacgtc ggcaacgggt taatgctcgc
      541 cgcgccacac acgggcgtga acgttcaagt tcaacccgtc tatctagatg gctacatcgg
      601 cgcggttcgc gtcggctgac gcaatagcat gaaacgaaag gcgcatagca ccgcgggagg
      661 tctgctgtgg gcggcgcacc ggccgtggca ccgggcctac tggtcgccga gtgctaccgg
      721 atcgaatcgc cgctaactgg cgagaccgag acagagcggg tcggcacact ctggcgggcc
      781 tatgacgagg tgctcgcgcg cccggtcgcg atcctgctcg cgcgaggcga cgacccgggc
      841 gtgcgagaag tgcttgcagg tgcacgcgca gcagccggac tgacgcacgc cgccgtcgtc
      901 cgcgtttatg acgcaggcga agccctgggg ctcgccttcg tcgtcaccga attcctgtcc
      961 ggcggttcgc tggaaagcca actgctgatc ggtccgctcg accccgccgt cgcagtggat
     1021 ctcgtctccg acctggcgat aggggttgcc gccgcacacc acgtcggctt gcacggcctg
     1081 tcgttgacac cacgcagcgt gctgttcacg tcaaccggcg caccacgttt ggtcggcatc
     1141 gcgctagccg gatataccgc gcgcagcgcg gcgccgccag ccctggctgt cagctcggat
     1201 gcggagcccc actacgcagg tgacgtcggc gaactcgagc gcgcccggat ggacgccgtc
     1261 ggcttggcca atctgctgta cgcgagcttg accgctcgat ggcccgggcc ggacgtcgac
     1321 agcgcgctgc caccggcccc tttcaccgaa ggtcacctgc gaaccccgcg tcaggtgcgc
     1381 ggcggggtgc cacgcgaggt cgatgaggtc gtcacgcagg ccttgggtga cgagacgctg
     1441 ctgcgggggc tgccggagat cgcgtcaccc gccgaattcg cggcagccct cgcacccctg
     1501 cgcgcgtcga ccgacgacgc ccacccgtat ggcgccgaca ccgggccgat caccgaactc
     1561 gagactcgcc agagccgctt ggtcaacacc cgcctcgtct cggtacaggt gtggcagcgc
     1621 tgggctcttg gcataggggt gttgttgctc gcggtcgtgg tcgcgggctt gctcctgggc
     1681 ggaaagcacg gctacctgag cttcgttcgc ggcgcggcac ccgcggcact tgaccccacc
     1741 ccgacggcga caccgaagac cacctcgagc gcagcggcgg tcgccgccgc cgagccgggt
     1801 gccatcgcgc ctggcctggt gaccgagttc gacccgtacg gcaaacacaa tgacccgcac
     1861 gtatcggagg ctccgcgcgc ggtcgacggg aatgctgcga cgatgtggcg gacccagatc
     1921 taccgaaccg ccgaccttgg caagctcaag cccggggttg gattgctgat ggacctcggc
     1981 agcccgcaga aggtcgcggc ggttcatctg cagctggtgg gcacgggcac gtcgctcgag
     2041 gttttcgaga gcaactctgc accggtcaac gagaaggcaa tgacgctcgc tgtggcccaa
     2101 gacggggtgc ccagcgacgt cacgttacgg ctcacgacca cgaccaccgc tcgctactgg
     2161 ctggtctggc tcaccaagct gcccgcaacc gggacggggt tccagggggg catcgccgaa
     2221 atgaccttcc tgtcgtgaat tctgttgtgg cgtcggtagg cttcgccgcg tgacggacgg
     2281 ggtgacggac gatccagacg ccgccttgct cacgcgtcac ctcgagggcg acccggccgc
     2341 gttcaccgag atcttccagc gccaccggga ccagctctgg gcagtggccc tgcgcacgac
     2401 aggtgacccc gaagacgcgg ccgacgcggt acaagaggcg ctcatctccg cgatgcgtca
     2461 cgcggcgtcg tttcgcggcg atgccaaagt cagcacctgg ctgcatcgga tcgtggtgaa
     2521 cgcctgcctt gaccgcttgc ggcgccgtgc cgcacatccg accagccccc ttccggacga
     2581 cgatcacgcg ccaagcacca cccgcgacga ctacgccgac cgcgacaccg cggccgatgt
     2641 gcatgccgca ctcgcgacct tgcccgccga gcagcgggca gcgatcgtgc tcgtcgacat
     2701 cgagggctac cccgtcgacg aggccgccgc gatgctcgac gtgccttcag gcacggtcaa
     2761 gagcaggtgc gcgcgtggtc gggcgaagct ggtgccgctg ctctcccatc tgaacggcgg
     2821 gc
//
LOCUS       DATCBJ010000369         2279 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_413935, whole genome shotgun sequence.
ACCESSION   DATCBJ010000369 DATCBJ010000000
VERSION     DATCBJ010000369.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2279)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2279)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2279
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_413935"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(531..710)
                     /locus_tag="VII50_07340"
     CDS             complement(531..710)
                     /locus_tag="VII50_07340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5050700.1"
                     /translation="MTVNGDVPRQKLTGMIGQQHQYVDLERCKIVGASGVLPQQAAQA
                     QWHDVIVPSRVVRRP"
     gene            complement(901..1677)
                     /locus_tag="VII50_07345"
     CDS             complement(901..1677)
                     /locus_tag="VII50_07345"
                     /inference="COORDINATES: protein motif:HMM:NF014090.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VIT family protein"
                     /protein_id="HEY5050701.1"
                     /translation="MTTRVSDERSTPTDAQVQAAAAGLATHSGEDHAGGSRGRLNWLR
                     AGVLGANDGIVSVAGIVLGVAGATSARGPIFTAGLAGLVAGGVSMALGEYVSVSSQRD
                     SEAAQLAQERRELAESPEQEIAELAALYMAKGLTAATAKQVAVELTDHDAFAAHVDAE
                     LGIDPEELAKPFQAAVASALAFTTGALLPLLSILLPPRAWRIPVTFGAVLVALGVAGA
                     VSARIGGSAAKRAVLRVLIGGAAGLALTYGIGHLFGTSVS"
     gene            1821..2063
                     /locus_tag="VII50_07350"
     CDS             1821..2063
                     /locus_tag="VII50_07350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5050702.1"
                     /translation="MAAHPARATRLPPSSKKARETGKIGTIEGLAGLSLDALTSVAYG
                     PEAIHRMLLNQRATILASVLRRHSTATIATFPFPLE"
BASE COUNT          417 a          795 c          736 g          331 t
ORIGIN      
        1 tggaccgggc cgtcgcgccg caataggtgc ctcgcgtgct cgccaagccg gcgggactgc
       61 ccgctcgatg ctcgattcag tgcggtacgt acggacccct gagggagcgg ggacgtacgt
      121 gccaccccga cacgccggga ccagcccgcc aagctggccc gacatcgccg tcgacgacaa
      181 ccagcgagcg cggtcagatc cgccggactg cctcgcgacc cagaggacgc gaacgacccg
      241 caatctcaca gagacggatt ggcccctgcc gatatggacg gccgcgaccg cgttgcgtgg
      301 aggagagccg ccagcccgcg ccccggcgca gggccccgag gcggctcgtg ggcggttgtg
      361 gacgtcaaag tgggtccagg tgcgcggcag ggtgagcggg catccgcgcg cactcaccgt
      421 ccgaggcgat aatcgccgag taagtagcag ccgttagcca gcgaaggtgt cagcggcatc
      481 gaacgtgaat cgtttgaggt tgccggcggt gagtgcaatg acacgaccct tcacggacga
      541 cggacgaccc gagatggcac gatgacgtcg tgccactgcg cctgtgcggc ttgctgtggg
      601 agcacgcccg acgcgccgac gatcttgcaa cgttcgaggt cgacgtactg gtgctgctgc
      661 ccgatcatcc cggtcagctt ctgtcgaggg acatcgccgt tgaccgtcac gacggtgatc
      721 cgcgcgaggt tcagctgatc gagtttccgg actacgcggc gctgagtgga taccaaagaa
      781 tccagcgccc accgatcggg cgcggacgcg caaccgcgat gcaatcatcg cccgaaccca
      841 gctgccacgc attcaaccct gcgacaggcc cggtcacctc tccgcagtgc ctcggtgtgg
      901 ttagctgacg gatgtcccaa atagatgacc gatgccgtac gtcagcgcga gcccggccgc
      961 gccgccgatc agcacccgca ataccgcgcg cttcgccgcg cttccgccga tccgggcgct
     1021 gacggctccg gcgactccca gcgcgaccag caccgcgccg aaggtgaccg gtatacgcca
     1081 agcgcgaggc ggcaacaaga tcgatagcag cggcaacagg gcacccgtgg tgaaggccaa
     1141 cgctgacgcc acggccgctt gaaacggttt cgcgagttcc tcggggtcga tccccagttc
     1201 ggcgtcgaca tgcgcggcga aggcatcgtg atcggtcaac tcaaccgcga cctgcttcgc
     1261 ggttgccgcg gtcagacctt tcgccatgta cagcgccgcg agctcggcga tttcctgctc
     1321 cggtgactcg gcgagttcgc ggcgttcttg ggcaagctgc gccgcttcgc tgtcgcgttg
     1381 actgctcacc gacacgtact cccccagtgc catcgacacc ccgccagcga ccaacccggc
     1441 aaggcctgcg gtgaagatgg ggccacgggc gctggtcgca ccagcgaccc cgagcacgat
     1501 gccggcgacc gaaacaatcc catcgttggc gcccaaaacg ccggcgcgta accagttcag
     1561 ccgtccgcgg ctaccgccag cgtgatcctc acccgagtgc gtagccagtc ccgccgccgc
     1621 agcctgcacc tgcgcatcgg ttggcgttga ccgctcatcg gacacacgcg tagtcaacca
     1681 gacggcccgt cccaccgagc cgcggtcgac gagaccgcgc cagggacctc gccgtggacc
     1741 agatctatgc ggcgcggcgg tgggtttggt gcgggcaacg acgctcagag aaccgatgtg
     1801 gtaggccgga ggaggcgcgt gtggcagccc atcctgcgcg cgcgacgcgg ctcccgccgt
     1861 cgtcgaagaa ggcgcgcgag accgggaaga tcggcacgat cgaaggccta gccgggttgt
     1921 cgttggacgc gttgacatcg gtggcgtacg gcccggaagc gatccaccgg atgctgctca
     1981 accaacgcgc aacgatcctc gcctcggtgc tccgccgaca cagcaccgcg acgatcgcca
     2041 ccttcccctt ccctttggaa tagcaacgcg cggcgtccgc accaggtcgc tgccgatgtc
     2101 gacacgacga gcgattgccc gccctcaacg cattcgcgat cacaggtccc accgacctcg
     2161 ggccacaaac gcgcaaagcg ggtccttagc ggggccgtac ggccggtaaa ctggggccta
     2221 gcacggacca cagcggtcaa gatcaaaaac agacatgcgc aggtcaacgg ccattttca
//
LOCUS       DATCBJ010000370         2449 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_314504, whole genome shotgun sequence.
ACCESSION   DATCBJ010000370 DATCBJ010000000
VERSION     DATCBJ010000370.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2449)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2449)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2449
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_314504"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..624
                     /locus_tag="VII50_07355"
     CDS             <1..624
                     /locus_tag="VII50_07355"
                     /EC_number="4.2.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009073319.1"
                     /note="catalyzes the formation of
                     4-imidazolone-5-propanoate from urocanate during histidine
                     metabolism; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urocanate hydratase"
                     /protein_id="HEY5050703.1"
                     /translation="AALSGDPADIAVTDRAVLELFPDNEPLERWIRSAQEKVAFQGLP
                     ARICWLGYGERHLAGLRFNELVARGEISAPIVIGRDHLDAGSVASPYRETEAMADGSD
                     AIADWPLLNALVNTASGASWVSIHHGGGVGIGRSIHAGQVSVADGTPLAAQKLERVLT
                     NDPGMGVIRHVDAGYELATEVAAQRGVRIPMHEGSVHEGSLHESAPE"
     gene            621..1910
                     /locus_tag="VII50_07360"
     CDS             621..1910
                     /locus_tag="VII50_07360"
                     /inference="COORDINATES: protein motif:HMM:NF006770.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016813 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear
                     amidines [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="allantoate amidohydrolase"
                     /protein_id="HEY5050704.1"
                     /translation="MSAAADIGGTFDRLWSELLPIGRTPVGGYRRFAWNDADLEARAW
                     FVAAAAARGLAVQSDRNGNLWAWWLPIGWLGDPRQAFVTGSHLDSVPDGGAFDGALGV
                     VCALCAIDLLRSRGVQPLRPIAVAVFADEEGARFGVACAGSRLATGALEPSAARALTD
                     VDGVSLHQAMRRAGVDTTQLGRDDETLRRVGIFVELHIEQGQALIDLDAPLGIGMGIW
                     PHGRWRFSFEGEANHAGTTRMTDRRDPMQPFAAAVLAARILASKHGGVATFGRVHVEP
                     NGTNAIPSTVTAWLDARGPDQAVLDAIVSGVSANAHARAAAERVELAVAGESVSAVVE
                     FGADANARLHRVLDSVAGLGVVACGGRVPDLGTGAGHDAGVLSSVGPTAMLFVRNPTG
                     VSHSPAEFAERDDCLAGVEALAAVMSDWVSTPPVEAS"
     gene            1913..>2449
                     /locus_tag="VII50_07365"
     CDS             1913..>2449
                     /locus_tag="VII50_07365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009073323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEY5050705.1"
                     /translation="MPSWWCENAWLPPGRVAARVLVTVDDGRITGVDEDVDAAPTAQR
                     LTGLVIPGLANAHSHAFHRALRGRTQAGRGDFWTWREQMYAVASRLDPDNYLALARAT
                     YTEMALAGITCVGEFHYVHQQPGGVPYSDPNAMGDALIEAAAQVGVRLTLLDTCYLAG
                     GIGEPLLPVQERFSDGDAA"
BASE COUNT          379 a          747 c          868 g          455 t
ORIGIN      
        1 gccgcgctct cgggcgaccc agccgacatt gcggtcacgg atcgcgcggt gcttgagctg
       61 tttcctgaca acgagccgct ggagcggtgg attcgatcgg ctcaggagaa agtcgctttc
      121 cagggcttac ccgcacgtat ttgttggctc ggttacggcg aacgccatct cgccgggctg
      181 cgcttcaacg aactggtggc ccgtggtgag atcagcgcgc ctatcgtcat cgggcgcgac
      241 cacctcgacg cgggatcggt cgcttcgccg taccgcgaga ccgaagcgat ggccgacggc
      301 tccgacgcga ttgccgactg gccgctgctg aatgcgctgg tcaatacagc atcgggtgcg
      361 tcatgggtct cgatccatca cggtggcggt gtgggcatcg gtcgctcgat tcacgcgggc
      421 caagtgagcg tcgccgacgg gaccccgctg gccgcacaaa aactcgagcg agtgttgaca
      481 aacgaccccg gcatgggcgt gatccggcac gtcgatgccg gctacgagct cgcgaccgag
      541 gttgcggcac aacgcggcgt ccggattccc atgcacgagg gttcagtgca cgagggttcg
      601 ctgcacgaat ctgcacccga gtgagcgcgg ctgccgacat cggtggcacg ttcgaccggc
      661 tctggtcgga gttgttgccg attggccgaa cccccgtggg cgggtatcgc aggtttgcct
      721 ggaacgacgc cgatctcgag gcacgggcgt ggttcgtcgc ggcagccgcc gcgcgcggtc
      781 tcgcggtgca gtcggatcgc aacggcaacc tgtgggcgtg gtggttaccg atcggttggt
      841 tgggcgatcc gcgccaagca ttcgtcactg gttcacacct tgattctgtg ccggacggcg
      901 gcgcgttcga tggtgcgcta ggagtggtct gcgcgctgtg tgcgatcgac ctgcttcgca
      961 gtcgcggcgt gcagccgttg cggccgatag ctgttgccgt atttgccgac gaggagggtg
     1021 cccgtttcgg agttgcctgc gctgggtctc ggctggcgac gggtgcgctg gaaccttcgg
     1081 cggcgcgggc gctcaccgat gtcgacggcg tgagcctcca ccaggcaatg cgacgcgctg
     1141 gtgtcgacac cacgcagctg ggccgcgacg acgagactct gcgcagggtc ggcatcttcg
     1201 tggagctgca tatcgagcaa ggacaagcgc tgattgatct cgacgcacca ctagggatcg
     1261 gcatgggtat ctggccacac ggccgatggc ggttctcctt tgagggcgaa gcaaaccacg
     1321 ctggtacgac gcgaatgact gaccgacgcg acccgatgca gccgtttgcc gccgcagttc
     1381 ttgcagctcg gatcctcgcc agcaaacacg gtggcgtcgc aacgttcggt cgcgtgcacg
     1441 tcgagccgaa cggcacgaac gcaatcccgt cgacggtgac ggcgtggctc gatgcgcgcg
     1501 ggccagatca ggcggttctg gacgcgatcg tcagtggcgt ctcggcgaac gcccacgcgc
     1561 gcgcggctgc cgagcgggtg gagctcgccg tggcaggtga gtcggtctca gcggtcgtcg
     1621 agttcggagc tgacgcgaat gcacggttac atcgggtgct cgactcggtg gcggggttgg
     1681 gtgtggtggc ttgcggcggt cgggtgccgg acctcggcac gggtgccgga cacgacgccg
     1741 gcgtgctgtc gtccgtgggc cctacggcga tgctgttcgt gcgtaatccg accggtgtct
     1801 cacactcgcc agcggaattc gctgagcgcg acgactgcct cgcaggtgtc gaggcgctcg
     1861 ccgcggtgat gtccgattgg gtcagcactc cgccggtcga ggcgagctga caatgccatc
     1921 gtggtggtgt gagaacgcat ggctgccgcc tggtcgggtc gcggctcgcg tcctggttac
     1981 cgtcgacgat ggtcgtatca ccggtgtcga tgaggacgtc gacgctgcgc caaccgcgca
     2041 gcggttgacc ggactcgtga tcccgggcct ggcgaacgcg cactcgcacg cgttccatcg
     2101 cgcactacgt ggccggacac aggcagggcg aggagacttc tggacgtggc gcgagcagat
     2161 gtacgccgtc gcgagtcgac tcgatcccga taactactta gcgcttgcgc gggcgacgta
     2221 caccgagatg gcgttggctg gcatcacttg tgttggcgaa ttccattacg tgcaccaaca
     2281 gcctggcggc gtgccctact ctgaccccaa cgccatgggc gacgcgctga tagaagcggc
     2341 ggcacaagtc ggcgtccgct tgacgttgct tgatacctgt tacttggcag gtgggatagg
     2401 cgaaccgctt ttgcctgtac aagagcgctt tagcgacggc gatgccgct
//
LOCUS       DATCBJ010000371         4586 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_296261, whole genome shotgun sequence.
ACCESSION   DATCBJ010000371 DATCBJ010000000
VERSION     DATCBJ010000371.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 4586)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4586)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4586
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_296261"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..499)
                     /locus_tag="VII50_07370"
     CDS             complement(<1..499)
                     /locus_tag="VII50_07370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016461948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate family dehydrogenase"
                     /protein_id="HEY5050706.1"
                     /translation="MTRSVRLAVIAGDGIGPEVVDEGLKVLDAVTPAFDVKVERTEYD
                     LGARRWHATGETLPDSVLDELRGHDAILLGAVGDPGVPSGVLERGLLLRLRFELDHFV
                     NLRPVKLFPGVVSPLAGVGPADIDFVVIREGTEGAYAGAGGFLRRGTPHEIATEESVN
                     TAYGVE"
     gene            complement(580..2172)
                     /gene="serA"
                     /locus_tag="VII50_07375"
     CDS             complement(580..2172)
                     /gene="serA"
                     /locus_tag="VII50_07375"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004617 - phosphoglycerate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HEY5050707.1"
                     /translation="MSKPIVLIAEELSPAGIDLLSGDFEIREVDGADRAALLPALATA
                     DAVIIRSATKIDAEAIAAAPNLKVVARAGVGLDNVDVKAATNAGVMVVNAPQSNIVSA
                     AEQAIALLFAVARRTPVANASLKSGQWKRSKFTGVEILDKTVGVVGLGKIGQLVAQRL
                     AAFGTHLIAYDPYVQPARAAQYGVRLVTLEELLREADIITIHLPKTPETVDLISEREL
                     GMVKPGVIIVNAARGGLVNEDALAIALKEGRIGGVGLDVFATEPTTSSPLFEFDNVVV
                     TPHLGASTDEAQEKAGTAVARSVKLALSGEFVPDAVNVQGGAIAEDVRPGLPLAEKLG
                     RVFTALAGGIATRIEVEVRGEIAAYDVKVLELAVLKGVFTDVVEKSVTYVNAPLFAQD
                     RGVEVSLVTSDDSPDFRNLITVRGTLPSGHQLSIGGTLVRPKFVEKLVKVDDFDVDLT
                     LSEHLAFFRYEDRPGVVGVVGRILGEHGINIAGMQVARDTRGGHALVALTVDSALPVA
                     VLETIVGEIGAESGRAVDLVEA"
     gene            2337..2576
                     /locus_tag="VII50_07380"
     CDS             2337..2576
                     /locus_tag="VII50_07380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5050708.1"
                     /translation="MGSNRVRTLMVFETLMFFNGLLCAGVGWILLIFMSKPAGLIGAL
                     IFWAVATLLWEGKRYVDRLLPFDGARRGRSRVRRG"
     gene            complement(2619..3620)
                     /gene="ilvC"
                     /locus_tag="VII50_07385"
     CDS             complement(2619..3620)
                     /gene="ilvC"
                     /locus_tag="VII50_07385"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007590324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HEY5050709.1"
                     /translation="MPVEIFYDDDVDLSIIQNRHVAVLGYGSQGHAHALSLRDSGVDV
                     RVGLLEGSKSRGKAESEGLRVLTPAAACEEADLVMVLAPDPVQRHLYKDAIEPNLVDG
                     DALFFGHGLNIRYGLISPPSGIDVCMVAPKGPGHLVRRQFEEGRGVPVLLAVEQDATG
                     HAWDLVKSYAKGIGGTRAGALKTTFKEETETDLFGEQVVLCGGLSELIKAGFETLVGA
                     GYQPEVAYFECLHEVKLIVDLMYEGGISKMYWSVSETAEYGGYTRGPRIVTNDTKAEM
                     QKILGEIQDGSFAREWMAEYDAGLPNYKRYYAEHVDHPIEVTGRALRPMMSWITDAE"
     gene            complement(3678..4202)
                     /gene="ilvN"
                     /locus_tag="VII50_07390"
     CDS             complement(3678..4202)
                     /gene="ilvN"
                     /locus_tag="VII50_07390"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004925060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HEY5050710.1"
                     /translation="MSKHTLSVLVENKPGVLARIAGLFARRGFNIDSLAVGPTEYADV
                     SRMTIVVNVEDNPLEQVTKQLNKLINVLKIVELEPSSSVSRELLLVKVKADSQSRSQV
                     LEIVQLFRAKVIDVAADAVTIEATGGEEKIDALLKVLEPFGVKELAQSGMVAMGRGTR
                     SITDRTLRPVDRIA"
     gene            complement(4224..4577)
                     /locus_tag="VII50_07395"
                     /pseudo
     CDS             complement(4224..4577)
                     /locus_tag="VII50_07395"
                     /inference="COORDINATES: protein motif:HMM:NF014794.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
BASE COUNT          752 a         1513 c         1502 g          819 t
ORIGIN      
        1 gttcgacgcc gtaagccgtg ttgacgcttt cttcagtggc gatctcgtgt ggcgtgcccc
       61 tgcgcaggaa cccacccgcg cctgcgtagg caccctccgt gccttcccgg atcaccacga
      121 agtcgatgtc ggccggacca acgccggcga gcgggctcac cacacctggg aacagcttca
      181 ccgggcgcag gttcacgaag tggtcgagtt cgaaccgcaa ccggagcagc aagccgcgct
      241 cgaggacccc gctgggcacc ccggggtcgc cgaccgcacc gagcaggatc gcgtcgtggc
      301 cgcgcagttc gtcgagcacg gagtcgggca gcgtctcacc ggtcgcgtgc cagcgccggg
      361 cgccgaggtc gtactcggtg cgctcgacct tcacgtcgaa ggcaggcgtc accgcatcga
      421 gaaccttcag cccctcgtcg accacctcag ggccgattcc gtcaccagcg atgacggcga
      481 gacgcacgct gcgagtcatc tcccaagggt accggccgtc tcagaaacgg tgcatcagat
      541 ctcaggatgc gagacgcggc cgcaccgcgg cgtccggaat caggcttcga cgaggtcgac
      601 ggcgcggccc gattcagcgc cgatctcgcc gacgatggtc tccaggaccg ccaccggcag
      661 ggccgaatca acagtcagcg cgacgagcgc gtggccgccg cgcgtatcgc gggccacctg
      721 catgccggcg atgttgatgc cgtgctcgcc gaggatgcga cccacgacac caacgactcc
      781 cggacggtcc tcgtagcgga agaacgcgag gtgctcggag agtgtgaggt cgacgtcgaa
      841 atcatcgacc ttgacaagct tctcgacgaa cttcggccgc acgagggtgc caccgatcga
      901 gagctggtga ccgctgggca gtgtgcctcg aactgtgatc aggttgcgga agtcggggct
      961 gtcgtcgctg gtcacgaggc tgacttcgac gccgcgatcc tgcgcgaaca gcggcgcgtt
     1021 gacataggtg accgacttct cgacgacgtc ggtgaatacg cccttcagca ccgcgagttc
     1081 gagtaccttg acgtcgtagg ccgcgatctc gccgcgaacc tcgacctcga tacgggtcgc
     1141 gattccgcca gcgagggcag tgaagacgcg gccgagcttc tcggcgagcg gcaggccagg
     1201 acgaacgtct tccgcgatcg cgccaccctg cacgttgacc gcgtcgggga cgaactcacc
     1261 agacagcgcg agcttgaccg accgcgcgac tgccgtaccg gccttctcct gagcctcgtc
     1321 ggtgctcgca ccgaggtgcg gagtcacgac gacgttgtcg aactcgaaca acgggctgga
     1381 ggtcgtgggc tcggtagcga acacgtcgag accgacaccg ccgatgcgac cctccttgag
     1441 ggcgatcgcg agagcgtctt cgttcaccag gccgccgcga gcggcgttga cgatgatgac
     1501 gccgggcttg accatgccga gctcgcgctc gctgatgagg tcgaccgtct cgggcgtctt
     1561 cggcaggtgg atggtgatga tgtcggcttc tcgcaggagc tcctcgagcg tgacgaggcg
     1621 tacgccgtac tgcgccgcac gggccggctg tacgtagggg tcgtacgcga tcaggtgggt
     1681 gccgaatgcg gccagccgct gcgcgacaag ttgtccgatc ttgcccagcc cgacgacacc
     1741 gacggtcttg tcgaggatct cgacgccggt gaacttcgaa cgcttccact gccccgactt
     1801 gagcgacgcg ttcgcgaccg gtgtgcggcg ggcgaccgcg aacaacaggg cgatggcctg
     1861 ctcggccgcg ctgacgatgt tcgactgcgg cgcgttgacc accatgacac cggcgttggt
     1921 cgcggccttc acgtcaacgt tgtcgaggcc gacgccggcg cgggcgacca ccttcaggtt
     1981 gggcgcagcc gcgatggcct cggcgtcgat cttggtcgcg ctgcggatga tgacagcgtc
     2041 agcagtcgcc agggcgggga gtagggcggc acggtcggcg ccgtcgactt cacggatctc
     2101 gaagtcgccg ctcagcaggt cgatgccggc gggcgagagt tcttcggcga tcaggacaat
     2161 gggcttgctc atgggtgggc aatcctccga cgtcatggct gggtcgtcgg cgttgggcca
     2221 ccacgctgta gcacgcgcct aatgggaagg attctagcgc gcaggtttcc ggcaggtttc
     2281 agccggctgt cgcgcgggtc tggcgtcggg aagcggtctt ggcgcacgat gaggtcgtgg
     2341 gatcgaaccg cgtgcggacc ctgatggtct tcgagacgct gatgttcttc aacgggctct
     2401 tgtgtgcggg cgtcgggtgg atcctcttga tcttcatgtc gaagcccgcc ggactcatcg
     2461 gcgccctcat cttctgggcg gtcgcgaccc tgctgtggga gggcaagcgc tacgtcgacc
     2521 ggctgctgcc cttcgacgga gcgcgccgcg gtcgcagtcg agtgcgccgc ggctgatcgc
     2581 cgtcccgccg tcttgccttc cctggactga cccgctgctt actcggcgtc ggtgatccag
     2641 ctcatcatcg ggcgcagtgc gcggccggtg acctcgatcg ggtggtcgac gtgctcggcg
     2701 tagtaccgct tgtagttcgg gagcccggcg tcgtactcgg ccatccactc gcgggcgaac
     2761 gacccgtcct ggatctcgcc gaggatcttc tgcatctcgg cctttgtgtc gttggtcacg
     2821 atgcgcgggc cgcgcgtgta gccgccgtat tcagcggtct cggacaccga ccaatacatc
     2881 ttcgagatgc cgccctcgta catcaggtcg acgatgagct taacctcgtg caggcactcg
     2941 aagtaggcga cctcaggctg gtatccggca ccgacgagag tctcgaagcc ggccttgatg
     3001 agctcggaaa gtccgccgca caacacaacc tgctcgccga acaagtcggt ctcggtctct
     3061 tccttgaacg tcgtcttcag tgcgccggca cgggtaccgc cgatcccctt tgcgtaggac
     3121 ttcacgaggt cccatgcatg gcccgtcgcg tcctgctcga cagcgagcag cacgggcacc
     3181 ccacgacctt cttcgaactg acgacgtacc agatgccccg gtcccttcgg cgccaccatg
     3241 cagacatcga tgccggacgg cggactgatc aggccgtagc ggatgttcag cccgtgcccg
     3301 aagaacagcg catcgccgtc gacgaggttg ggctcgatcg cgtccttgta gaggtggcgc
     3361 tgaaccgggt cgggcgcgag caccatgacc aggtcggcct cctcgcacgc ggctgccgga
     3421 gtgagaacgc gcaatccctc cgactccgcc ttgcccctgc tcttcgagcc ctcgagcaga
     3481 cccacacgca cgtcaacgcc ggagtcgcga agcgacagag cgtgtgcgtg cccctggctg
     3541 ccgtatccga gcacagcgac gtggcggttc tggatgatcg acaggtcaac gtcgtcgtcg
     3601 tagaagatct caacaggcac ggacgtgcac tccttcgctc ggtttctctg ttggcggtat
     3661 tacgcgacca ggtggtgtta cgcgatccgg tcgactgggc gcagtgtgcg gtcggtgatc
     3721 gagcgcgtgc cgcggcccat tgcgaccatg cccgactgcg cgagctcctt cacgccgaac
     3781 ggctcgagca ccttgagcaa cgcatcgatc ttctcctcgc cgccggtggc ctcgatggtc
     3841 accgcgtcag cagcaacgtc gatcaccttc gcccggaaca gttgcacgat ctccagcacc
     3901 tgcgaccggc tctgcgaatc ggccttcacc ttgaccagga gcagttcgcg cgacaccgac
     3961 gaggacggct cgagctcgac gatcttgagc acgttgatca gcttgttgag ctgcttggtg
     4021 acctgctcga gcgggttgtc ctcgacgttg accacgatcg tcatccgcga cacgtccgcg
     4081 tattcggtcg gcccgaccgc gagtgagtca atgttgaaac cgcgccgggc gaacaggcca
     4141 gcgatccggg ccaggacacc tggcttgttc tcgacgagca ccgagagcgt gtgcttggac
     4201 atgacttaga gttcctctcc gtcccaggca ggtgcgaggt cccgggcgac cttgatgtcg
     4261 tcgttgctcg tgccggcggc gaccatcggc cagaccatgg cgtccttgtg cacgccgaag
     4321 tcgatcacga cgggtgcgtc attgatgccc atcgccttct cgatcgtcgc atcgacgtcg
     4381 tccgcgctgt cgcatcgaag accgacacag cccatcgcgt cagcaagctt cacgaagtcc
     4441 ggaatccggc gcgagtgcag gtcggtgttg gagtagcgcg cgttgtagaa gagcgtctgc
     4501 cactggcgca ccatgcccag cgcaccgttg ttgatcagcg caaccttgat cggcgcgcct
     4561 tcgagtgcgc acgtcaccag ctcctg
//
LOCUS       DATCBJ010000372         3783 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_336429, whole genome shotgun sequence.
ACCESSION   DATCBJ010000372 DATCBJ010000000
VERSION     DATCBJ010000372.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 3783)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3783)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3783
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_336429"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..481)
                     /locus_tag="VII50_07400"
     CDS             complement(<1..481)
                     /locus_tag="VII50_07400"
                     /inference="COORDINATES: protein motif:HMM:NF015651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase
                     domain-containing protein"
                     /protein_id="HEY5050711.1"
                     /translation="MSSPAAPRRGLAWRSAISGTALLALIVWVIADHAKLSQAVAQLS
                     ALPGPSWSAILIAVGAVVASYCCSAVALSAAAGRRLPAGRTILVQFAAAAANRVTPGG
                     VGGMAVNARFLRTQKLSSGTVGAALSLSAMAHIGIATVGVLAMGPTITRLPIAHSLVT
                     "
     gene            785..1048
                     /locus_tag="VII50_07405"
     CDS             785..1048
                     /locus_tag="VII50_07405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5050712.1"
                     /translation="MTTRADGMRRIGTANATIATVSIAGVVGLSVMLAQQSTHHTAAI
                     TPTAAISTSTSSDDTTTTVTSIPQATVQQSTSGSHHHAVSSGS"
     gene            1045..1992
                     /locus_tag="VII50_07410"
     CDS             1045..1992
                     /locus_tag="VII50_07410"
                     /inference="COORDINATES: protein motif:HMM:NF014477.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HEY5050713.1"
                     /translation="MTLPTAQWPAIGTTAQVVVTDPAYLDAAQQIVVEELAQLDLACS
                     RFRSDSEIARLAARGNAWTAVSDVLAEVLACALDVARVTDGDVDPTMGTDMSRLGYDR
                     DFGEMRTASATGSDNAVLPITYRLVYRTTWHDVELDHAKRRVRVPAGLVLDLGAIAKA
                     WCADRCAVRIHRELGTGVLVSLGGDIATAGTGPDGGWVVRVQDLPDARTGPSALVVLS
                     DGGAVATSSTITRTWQRGGFRLHHILDPATRRPAAPIWRTVTAVANTCVDANAATTAA
                     IVRGRAAQAWLVSRGVAARLVDYDDRVHILGGWPAEVAA"
     gene            1989..2603
                     /locus_tag="VII50_07415"
     CDS             1989..2603
                     /locus_tag="VII50_07415"
                     /inference="COORDINATES: protein motif:HMM:NF013918.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric reductase-like transmembrane
                     domain-containing protein"
                     /protein_id="HEY5050714.1"
                     /translation="MSTASIDKALWYLGRSSGVVSLVLLTLVVALGIATRSGRLLPGL
                     PRFGVSAVHRSASLLAVVFLATHVVTLTLDPQSQLRWIDAVVPFGSHPKPLWIGLGAA
                     SLDLMIALVVTSLLRQRIGHRVWRAIHWTAYALWPFAVLHTIGTGTDMHSPWMVGLVA
                     ACSLTVAGLIGWRLSTRFLDATDRATGTQLPSRETRLLTSSKGL"
     gene            2603..>3783
                     /locus_tag="VII50_07420"
     CDS             2603..>3783
                     /locus_tag="VII50_07420"
                     /inference="COORDINATES: protein motif:HMM:NF013662.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-ubiquinone oxidoreductase-F iron-sulfur
                     binding region domain-containing protein"
                     /protein_id="HEY5050715.1"
                     /translation="MTTLFDEGVQTDASVPARGALVGPSPASQRELQAHLVAHGAVPV
                     MDAHAVVQLAVDSGLAGHGGAGFPLYRKLEAVNASRGRTIVVANGAEGEPASAKDRWL
                     LARRPHLVLDGLQLVARATKATDAYLYVNHRVPVAMLMDVLAERRRAGLEHVSVTVVA
                     APDVFISGEETAVVSHLSGSAALPRFKSPRVTERGVRGRPTLVSNVETLALLALIARR
                     GAAWFRAMGTTDSPGTMLLTVGGAVRAPGIVEAGHGTPLADILGLAGGPSTHLQAVLL
                     GGYHGTWLTTDEFASAKLSRPNLAPLGASPGAGVVVALSRGTCGLVEMSRVMSYLAEE
                     SAGQCGPCLNGLPRIANQLKSVAFGRGDRRAVDEMLRLGGMVVGRGACHHPDGSMRFL
                     R"
BASE COUNT          656 a         1227 c         1217 g          683 t
ORIGIN      
        1 ccgtgacaag tgagtgtgct atcggcagcc tggtgatcgt cggtcccatc gcgagaacgc
       61 cgacagtagc gatcccgatg tgggccattg ccgatagcga tagcgcagcg cctacagttc
      121 ccgacgacaa tttctgcgta cgtaagaatc gcgcattgac cgccatgcca ccgacaccac
      181 ccggcgtcac tcggttggcg gccgcggccg cgaactgcac aaggatcgtg cgaccggccg
      241 gcaatcgccg accagcagcc gccgacaaag cgaccgcgga gcagcagtag ctcgcaacca
      301 cggcgcccac tgcaatgagg attgccgacc acgatggacc aggcaacgcc gagagctgcg
      361 caacggcttg actaagcttt gcgtgatcgg caatcaccca cacgatcagt gcgagaagcg
      421 ctgttccgct gatggcggag cgccatgcga gaccacgacg cggcgcggca ggactcgaca
      481 tccttccact gtgcctgacc gaccttacga acgggtgaca agataacgcg cccgccagca
      541 gtcctggcta agccctatta acgcgccctg ttaacgccgt tgtcaggtca gccgtgcatg
      601 gtggagatcg aagccatagc ccaagttcaa cggggattct caggaatctg tgttcaacgg
      661 gattctcagg aatctgtcag tcggctttag gccacctgtc aggcaggtgc ctcaatgtag
      721 gggtcaacgc ggttagcgaa agcaaccgaa ctgtacttac caagtcgctg cacaggaggt
      781 cgcaatgaca acgcgcgctg acggcatgcg ccggataggc acggccaacg caaccatcgc
      841 aacggtgagt atcgctggcg tcgtcggcct gagcgtgatg cttgcccagc aaagcaccca
      901 tcacaccgcg gcaatcaccc cgaccgccgc gataagcacg tcgacgtcct cagacgacac
      961 cacaacgact gtcacctcta tcccccaggc cacggttcag cagtcgacgt cggggtcaca
     1021 ccatcatgcg gtttcgagcg gatcatgacg cttcccaccg cacagtggcc cgccatcgga
     1081 acaaccgcac aggtcgtcgt cactgacccg gcatacctcg acgccgcaca acagattgtc
     1141 gtcgaggagt tggcgcagct ggatctcgct tgcagccgat tccggtccga ttctgagatc
     1201 gctcgactcg ctgcacgcgg aaatgcatgg acggcagtga gcgacgtgct tgccgaggtc
     1261 ttggcgtgcg cactcgacgt cgcgcgggtt accgacggtg acgtcgatcc cactatgggc
     1321 acggacatga gccgtctcgg ctacgaccgc gacttcggcg aaatgagaac ggcatccgcg
     1381 acaggctcag acaacgcggt gctgcctatt acctatcgcc tcgtgtaccg gacgacgtgg
     1441 cacgacgtcg agttagacca cgccaaacga cgggtccgcg taccggccgg actcgtcctt
     1501 gatctcgggg caattgccaa ggcgtggtgt gcagatcggt gcgcggttcg aatccaccgc
     1561 gaactcggca ccggcgtgct cgtgagcctg ggcggcgata tcgcaacggc cggaaccggt
     1621 ccggacggcg ggtgggtggt acgtgtgcag gatctgccgg acgccagaac tggcccgagc
     1681 gcgctcgttg ttctcagtga cggaggtgca gtcgcgacgt cgagcacgat cactcgtact
     1741 tggcagcgtg gcgggtttcg gcttcaccac attctcgacc cggcgactcg gcgtccggct
     1801 gcgccgatct ggcgaacggt gactgctgtc gccaacactt gcgtcgatgc gaatgccgca
     1861 actacggccg cgattgtgcg cgggcgcgct gcacaagctt ggcttgtgag tcgcggggta
     1921 gccgcacgtc ttgtcgacta cgacgaccgc gtgcacattc ttggtggatg gccagcggag
     1981 gttgcggcat gagcaccgca tcgatcgaca aggcattgtg gtacctcgga cggtcgagtg
     2041 gcgtcgtcag tcttgtgctg ctcacgctgg tcgtcgccct cggcatcgcc acccgttctg
     2101 gccggcttct tcccgggctt ccgcgattcg gcgtaagtgc ggtgcatcgc tccgcaagct
     2161 tgctcgccgt cgtatttctg gcgacgcacg tggtgacgct cacgctcgac ccgcagtcgc
     2221 agctgcgctg gatcgacgcc gtcgttccct tcggcagcca cccgaagcca ctgtggatcg
     2281 ggctcggcgc cgcgtcactc gatctcatga tcgcgcttgt cgtgacgagc ctgctgcgtc
     2341 agcgcatcgg gcatcgcgtc tggcgggcaa ttcattggac ggcgtacgca ctgtggccat
     2401 tcgccgtcct gcacacgatc ggcacgggta ccgacatgca cagcccgtgg atggtcggac
     2461 tcgtcgccgc ctgctcgctc acggttgccg gcttgatcgg gtggcggttg tcgacccgat
     2521 ttctcgacgc aaccgatcgg gcgaccggca cccaactgcc atcccgcgaa acccgcctac
     2581 tcaccagttc gaagggcctc tgatgacaac cctgttcgac gaaggcgttc agaccgatgc
     2641 ctcggtccct gcgcgcggcg cccttgtggg gccttcgccc gcatcccaac gtgagttgca
     2701 ggctcacctc gttgcacacg gtgcagtacc cgtcatggat gcgcacgcgg tggtgcagct
     2761 cgccgtcgac agcggtctcg ccgggcacgg cggtgcgggc ttccccctgt atcgcaagct
     2821 tgaagcggtg aatgcgtcgc gcggccgcac gatcgttgtt gcgaacggcg ccgaaggcga
     2881 accggccagt gctaaggatc gatggctgct tgctcggcgt ccgcatctcg tgctcgatgg
     2941 tctgcagctt gtcgctcgag cgacgaaggc aaccgacgcg tatctctatg tcaaccaccg
     3001 ggtgccggtc gcgatgttga tggatgtgct cgcggagcga cgtcgtgcgg gattggagca
     3061 tgtgtcggtc acggtcgttg cggcgcctga cgtgttcatc tcgggcgaag aaacggcggt
     3121 cgtgagccac ctgtccggca gcgcggcact cccgcgcttc aagtcgccca gggtcaccga
     3181 acgtggggtg cgcggccgcc cgacgctggt aagcaatgtg gaaacgcttg ccctgctggc
     3241 gctgatcgcc cgacgcggcg cggcgtggtt ccgtgcaatg ggcacaaccg actcaccggg
     3301 cacgatgctg ctcaccgtcg gcggtgcagt tcgtgcgccg ggcattgtcg aggcaggcca
     3361 cggcacgcct ttggccgaca tactcggtct ggcgggcggg ccgtcgacgc accttcaagc
     3421 cgtgcttctc gggggctatc acggtacctg gttgaccacc gacgaattcg cctccgcgaa
     3481 actgtcgcga cccaatctcg cgccgctcgg cgcctcgccc ggcgcaggcg tcgtggttgc
     3541 actgtcccgc ggaacgtgcg ggctcgtgga gatgagcaga gtgatgagct atctcgctga
     3601 ggagtcggcc gggcaatgtg ggccgtgcct gaacggcttg ccgcgcatcg cgaaccagct
     3661 caagtctgtc gcgttcgggc gcggcgatcg acgtgctgtc gacgagatgc tgcggctggg
     3721 aggaatggtc gtcggccgcg gcgcatgcca ccaccccgat ggcagcatgc gcttcctgcg
     3781 tag
//
LOCUS       DATCBJ010000373         2020 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_333170, whole genome shotgun sequence.
ACCESSION   DATCBJ010000373 DATCBJ010000000
VERSION     DATCBJ010000373.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2020)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2020)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2020
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_333170"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..513
                     /locus_tag="VII50_07425"
     CDS             <1..513
                     /locus_tag="VII50_07425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009075614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEY5050716.1"
                     /translation="VVLDRDLPIVHGDDVCRRIIADGSNSRVIMLTAAGALEERVGGL
                     GLGADDYLPKPFAFVELVARIRALARRGGTPVPPTLVNGDLRVDSALRTAVRAGRPLE
                     LTRKELAVLEYLLAAGGRPVTAEELLEHVWDEAADPFTTTVKVTMRRLRTKLGEPPVI
                     ETVAQGGYRI"
     gene            561..1841
                     /locus_tag="VII50_07430"
     CDS             561..1841
                     /locus_tag="VII50_07430"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY5050717.1"
                     /translation="MSRRRIAPHLPRRTVRRRLTVLYGGVFVVSGAALLTITNLLVRH
                     TTGNFLFIQSSSAGSTTGIIAGGGSGPGAVSAVPRAGGGPDQAQQQAAQLIAQIRHQH
                     TIEMHQLLIQSFVALAIMSVLSIGLGWLVAGRALRPLRTITAAARDISSSNLHERLAL
                     SGPDDELKELGDTFDALIARLERSFRSQRQFVANASHELRTPLTLQHALLEAALLDPN
                     ETLHGWRATGERALAASQEQERLIAALLTLARSERGLSRHEPFDLALLTASVLSGRQS
                     EAEQRGLRVSGRLETAWTVGDPELAERLIANLIDNALRHNVADGQAEVVTESVGGHAV
                     VSVRNTGPVLAPLEIDRLFEPFQRADVNRTSHNGGLGLGLSIVQAIAAAHDADVSARA
                     RPTGGLDVEVRFPVRVHRAAVPPNEESTVSAVAG"
BASE COUNT          363 a          645 c          649 g          363 t
ORIGIN      
        1 gtcgtcttgg atcgcgacct tccgatcgtg cacggtgacg atgtgtgccg gcgcatcatc
       61 gctgacggat cgaacagtcg agtgatcatg ctgacggcgg ccggcgctct tgaagagcga
      121 gtgggcggac tcggacttgg cgccgacgac tacctgccca agccgttcgc gttcgtcgag
      181 ctggtggcac gcatccgggc actagcgagg cggggcggga cgccggtgcc gccgaccctc
      241 gtcaacggtg acctgcgcgt cgactcggcg ttgcgcaccg ccgtccgcgc tggtcgtccg
      301 ctggaactga cccggaaaga actcgccgtc ctcgagtatc tgctcgcggc aggaggacgt
      361 ccggtaacgg ccgaagagtt gctggaacac gtctgggacg aagccgcgga cccgtttacg
      421 actaccgtca aggtgacgat gcggcggctg cggaccaagc tcggcgagcc gccggtgatc
      481 gaaacggtcg cgcagggtgg ataccgaata tgagtggtta ccgaatatga gtggttaccg
      541 aacatgagtg gttaccgact atgagtcgtc gacgcatcgc gccgcatcta ccgcgccgaa
      601 cagtccggcg ccggctcacc gttctctacg gcggcgtatt cgttgtgtca ggtgccgcac
      661 ttctgaccat caccaatctg ttggtgcgac acacgactgg caacttcctc ttcattcaaa
      721 gcagtagcgc aggcagtacc acagggatca tcgcgggtgg cggcagtggg cctggcgcgg
      781 tgtcggccgt accgcgggct ggcggaggac cagaccaggc ccaacagcaa gcggcgcaac
      841 tcattgccca gattcgtcat cagcacacca ttgagatgca ccaattgctg atccaatcgt
      901 ttgtcgcgct cgccatcatg tcggtacttt ctatcggcct cggctggctg gttgctggtc
      961 gcgcgctgcg cccattgcga acgatcaccg cggctgcgcg cgacatctcg tcgagcaact
     1021 tgcacgaacg gctggcattg tctggacctg acgacgaact caaggaactc ggcgacacgt
     1081 tcgacgcgct gatcgctcga ctcgagcgtt cgttccgatc gcagcggcag ttcgtcgcga
     1141 acgcatcgca tgagctccgc acgccgctga cattgcagca cgcgttgctg gaggctgcgc
     1201 tcctcgatcc caacgagact ctgcacggct ggcgggcgac cggcgaacgc gcactcgcgg
     1261 cgagccagga gcaggagcgc ctcatcgctg cactactcac gctcgcgcga agcgaacggg
     1321 ggctcagtcg tcacgaacca ttcgacctag cgctgctaac agcttccgtg ttgagcggcc
     1381 ggcagagcga agctgagcaa cggggactgc gtgtttccgg tcgccttgag actgcgtgga
     1441 cggtcggtga cccggagctc gctgagcgac tcatcgccaa cctcatcgac aacgccttgc
     1501 gtcacaacgt tgccgacggt caggccgaag ttgtgacgga gtcggttggc ggccacgcgg
     1561 tcgtctcggt tcgcaacacc ggtccagttc ttgcgccgct ggagatcgac cggttgttcg
     1621 agccgttcca gcgcgcggat gtcaaccgca ccagccacaa cggcggcctc ggtctcgggc
     1681 tctcgatcgt gcaggcgatc gctgccgcac acgacgcgga cgtcagcgcg cgagcacgac
     1741 cgaccggagg actggacgtc gaagtccgct tccccgtccg cgttcatcgt gcggccgtcc
     1801 cacccaacga ggagagcact gtctccgccg tcgccggttg atcacagcag ccgtcggctt
     1861 gatcagccgc gtgagcgaag atcgttgaag gccctggagt actcggggac gcacggatag
     1921 tcgctatgcg tgagaacctt gagcatgtag ccggtttcgt cgactcttcg gtcatcaacc
     1981 ccaaaacggg cgtcgaagtc atcgctggcg caaaccgtag
//
LOCUS       DATCBJ010000374         5350 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_2377, whole genome shotgun sequence.
ACCESSION   DATCBJ010000374 DATCBJ010000000
VERSION     DATCBJ010000374.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 5350)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5350)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5350
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2377"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(478..1026)
                     /locus_tag="VII50_07435"
     CDS             complement(478..1026)
                     /locus_tag="VII50_07435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5050718.1"
                     /translation="MSSLDEREWLNDELRAQVRPPPARFRVTISVAFIVAAVGAVVLS
                     QIGFLHPRVGASFGDASAQGNRLVVVLVVYNSAQVPVRVTGFRAAQPGARIVSARFNG
                     AFPPAPDNNPAPDGARVAPFTVHEQQPLETVTIVYLLDCAKLRPPLRVNIGTHTFFGN
                     QTSTATLDSSTSAPLKQVCPGA"
     gene            1114..2019
                     /locus_tag="VII50_07440"
     CDS             1114..2019
                     /locus_tag="VII50_07440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5050719.1"
                     /translation="MNAVAIFVILTALACFLACCLALPWWPLSSLDRATVAEFAAAHN
                     LALTPASGRYVVGYLVRNLRWRRWGGFAGLALALVLSNAMTHTATTTTSAGQDTTITS
                     THTSHSLSWFLLIGIGYFGGALIAEWRSGATITGPRRAASLQVRDARDYLDPTFRRTT
                     YRALAAGALLVTAAASWPGYRQSTSLAERIALLALLLLVVVVTQVATQWVARRPQPLG
                     DDLLTAREAVRTSCVNLLGGIGVAFAWFVLSWVASSSRAHSPGVINPVASLTTIVAFV
                     MSVRCWFRLRRRAWTTDFRHPRVVA"
     gene            2046..2399
                     /locus_tag="VII50_07445"
     CDS             2046..2399
                     /locus_tag="VII50_07445"
                     /inference="COORDINATES: protein motif:HMM:NF012610.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEY5050720.1"
                     /translation="MPPYEQLRAQIATLAASGVLRGGDRLPTIRQLAGDLGLAAGTVA
                     RAYTELEAAGVLTTHGRRGTFVVEHTRANPSISRSERDRRLSEAADAYALAAHQLGVD
                     RKAAIRHAQAALARR"
     gene            complement(2423..3142)
                     /locus_tag="VII50_07450"
     CDS             complement(2423..3142)
                     /locus_tag="VII50_07450"
                     /inference="COORDINATES: protein motif:HMM:NF016300.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HEY5050721.1"
                     /translation="MADPIGLLLSVRGEARVVVAPDSVVLSCALTVSRTSKAEAMAGA
                     AAASRRLTADLASLGGVSLGVDTEQAALTWSARSATSWEESQHNKLTGLDEPSERVNA
                     TVELQVTTRTFDILDALGAHLGTHKELHVRQVNWRVNSDNPAWAQVRAAAIHAAIATG
                     RDYATALGVSLLEVAHVADAGLLDGEMSLTGGNRPRGAMMSAGGGSSLADTPSLDPEP
                     QELTATIDARLRATAVTLTGS"
     gene            complement(3225..3896)
                     /locus_tag="VII50_07455"
     CDS             complement(3225..3896)
                     /locus_tag="VII50_07455"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEY5050722.1"
                     /translation="MGPSQILIHSPFLGPATWQSTARELRAKDRRAHVPSLLAVAQST
                     PPYWPAGVDSIIRAAADEPVVLVPHSNAGLYVPAVVEALGEQVRGVVFIDAALPGAGH
                     HTTSDFLGRLAIVDGLLPPWTSWWDEADIDALFPDAYVRAEVETEQPRMPLAYYDHLP
                     SAPYDWSAPSVGYIWFGKPYDKGAAQAAQRGWPTTHLPGGHLHMLSDPEAVAAAVLDM
                     SGDWL"
     gene            complement(3954..4526)
                     /locus_tag="VII50_07460"
     CDS             complement(3954..4526)
                     /locus_tag="VII50_07460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5050723.1"
                     /translation="MTGVERLYQRWGLWRFVVLSVVFWVLLWPVGVGWLVVAAIRRYR
                     GLDKSPAARTARRIERFLSWYPAQWRARYGQDLEQLLHDTIESGHGGVRLTLNMVRES
                     AAAHHTSTGNTIGALCWSLCWIPLVPQGAVPLIFKLTGVSTRSWFLATYLPAPFQWPT
                     IAAMLGVGLTMLATAARNNRHRRYERTSRG"
     gene            complement(4523..4876)
                     /locus_tag="VII50_07465"
     CDS             complement(4523..4876)
                     /locus_tag="VII50_07465"
                     /inference="COORDINATES: protein motif:HMM:NF015513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEY5050724.1"
                     /translation="MSRSENGLGALGHYAGPATLILSSLAGGPKHGYALTADIEGFAG
                     VRLQPGTLYGALTRLEKQGLIESLEADGRRLPYRLTGDGAAALHAHLTAQAQTAQFGL
                     RRLAVSWSPPTTVSS"
     gene            5003..>5350
                     /locus_tag="VII50_07470"
     CDS             5003..>5350
                     /locus_tag="VII50_07470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5050725.1"
                     /translation="MGVLREWQCDEAPMQLHPGDLGWFWRFGAEATAAAVRTWSRDGQ
                     ILAVGLLDGPTLLRLTVAPGAERDEELAQQLVNDVTDPARGVLPAGKVSVEAPMGALV
                     QDLLFEDGWNADEP"
BASE COUNT          928 a         1727 c         1781 g          914 t
ORIGIN      
        1 aactcgcatc tacgagatgc ccttcgtgag ccacggaacg ggaccttaga caagcccaat
       61 tctgttgcca cgcaagggca ttctcgtgtt ccgacccgcc cagaccaaca agatcatcgg
      121 tggatcaggg ctaagacgtg caacccatcc cgacgaccag gaccacggag aaaccggcag
      181 accgggaaca ccgcgacgcc caaaatcagt cacaccccga aaccgccaaa cacttccgca
      241 gcccgaggcc tacaggcatc cggacggtgg atccgggcta agcgtggagc ttgaaaatgc
      301 ttgagttatt gagcgggcgg ttggcgttgc ttgcgcatcc gccggtcgcg attgctgcga
      361 gtaggcaggc gacggtcatc gatgttgctg gcacagttct tggacgtgac ggcaaagccg
      421 aatggttcca cgaaatgcac gagccgaggc ccggtatctc acattctgta ggatccgtta
      481 ggcccctgga catacttgtt tcagtggcgc gctcgtcgag ctatccagcg ttgcggtgct
      541 cgtctggttg ccgaaaaagg tgtgggttcc gatgttgact cgcagcggcg gacgcagctt
      601 cgcacagtcg agtaggtaga cgatggtgac cgtttccaag ggctgctgtt cgtgcacggt
      661 gaagggtgct acgcgggcgc cgtcgggagc cggattgttg tcaggggcgg gcgggaaagc
      721 gccgttgaaa cgggcgctaa cgatgcgagc accgggttgt gctgcgcgaa acccggtgac
      781 gcgcaccggt acctgcgcgc tgttgtacac cacgagcacg acgacgagcc ggttgccttg
      841 agcgctcgcg tcgccaaacg acgcgccaac tcgcgggtgc aagaagccga tctgcgacaa
      901 gaccacggcg ccgacggcgg cgacgatgaa cgccactgag atcgtgaccc ggaaccgggc
      961 aggtggtggg cgaacctggg cccgcagctc gtcattgagc cattcccgct cgtcgagtga
     1021 cgacacccct ggctccttcc gtctgtatca gtgacatgat acaaagtggc aggtcgttgt
     1081 cgttcacgca agtcatgctc tgcaaggagt cgcatgaatg cggttgccat tttcgtcatt
     1141 ctcacagcgc tggcctgctt tctggcctgc tgtttggcgt taccgtggtg gccgttgagt
     1201 tcccttgacc gcgcgaccgt cgccgaattc gcagcagctc acaacctcgc attgacgccg
     1261 gcgagcgggc gctacgtcgt cggctacctc gtgcgtaacc tgcgctggcg tcgctggggc
     1321 ggcttcgcgg gtctggcgct ggcactggtc ctgtcgaacg caatgaccca caccgccacg
     1381 acgaccacct ccgcgggcca ggacacgacc atcacctcga cacacaccag ccattcgctt
     1441 agctggttcc tgctgatcgg catcggctac ttcggcggcg cactgatcgc agagtggcga
     1501 agcggtgcca cgatcaccgg gccacgacgc gccgcctcat tgcaagtgcg cgacgcccgt
     1561 gactatctcg atcccacgtt caggcggacc acgtacagag cgttggccgc gggcgcgctg
     1621 cttgtcacgg cggcggcatc gtggcccggt taccggcaat cgacatcgct ggctgagcga
     1681 atcgccttgc tggcattgct tcttcttgtc gtcgtcgtca cgcaggtggc aacgcagtgg
     1741 gtggcacggc ggccgcaacc tctcggtgac gacctgctca cggcacgcga ggcggtacgc
     1801 accagttgcg tcaacctgct cggtggcatc ggcgttgcat tcgcctggtt cgtgctgtcc
     1861 tgggtagcgt cgagttcccg cgcgcacagc cctggagtga tcaatcccgt ggcgagcttg
     1921 acgacgatcg tcgcgttcgt catgtccgtg cgctgctggt ttcggctccg tcgtcgcgcc
     1981 tggacgaccg acttccgaca tccgcgagtc gttgcttgat agtcgcggtc gatgcgcaat
     2041 ctccagtgcc gccgtacgaa caactgcgcg cacagatcgc gacgctcgcc gccagcggcg
     2101 tactacgcgg cggcgaccga cttccgacga tccgacaact cgcaggcgac ctgggcctcg
     2161 ccgccgggac ggtcgcacgc gcctacaccg agttagaagc tgccggggtg ctgacgacgc
     2221 acggcaggcg aggaaccttc gtcgtcgaac acacgagagc gaaccccagc atctctcgca
     2281 gcgaacgcga ccgtcgcctc agcgaagctg ccgacgccta cgcactagct gcgcaccaac
     2341 tcggcgtcga ccgcaaagcc gccatccgac acgcccaagc tgccctcgcg agaaggtagc
     2401 cgcgcggtgg ttgcgcgccc catcagctgc cggtcagggt cactgcggtt gcgcgcaggc
     2461 gggcgtctat ggtcgcggtg agttcttggg gttctgggtc cagtgatggc gtgtcagcga
     2521 gtgagctgcc accgccagca gacatcatcg ccccgcgcgg ccggtttcca ccagtcaggc
     2581 tcatctcgcc gtccaagagc ccggcgtcgg cgacgtgggc cacctccaat aaggagacac
     2641 cgagcgccgt cgcatagtcg cggcctgtag cgatggcagc gtggatcgct gcggcccgta
     2701 cttgcgccca cgcagggttg tcggagttga cccgccaatt gacttggcgt acgtgtagtt
     2761 ccttgtgggt gccgaggtgc gcgccgagcg cgtcaaggat gtcgaaggtg cgcgtagtga
     2821 cctgcagctc gacggtcgcg ttgaccctct cgctgggttc gtcaaggccg gtgagcttgt
     2881 tgtgctggct ttcttcccag cttgtcgccg accgcgcgga ccaggtcagc gccgcctgtt
     2941 cggtgtcgac cccaagactg acgccaccca atgaggccag gtcggcggtc agccggcgcg
     3001 atgccgcggc ggctcccgcc atcgcctcgg ccttggacgt gcgtgacacc gtgagtgcgc
     3061 aagacagcac gacggagtcg ggagctacca cgacccgtgc ctcgccacgc accgacaaca
     3121 gtaacccgat cggatcggcc atcgagaccc ccctcagccg agcctcctcg gcacagcgat
     3181 gccacacccg gccagacgca gcacgtccca ctatccatcg agggtcaaag ccagtcgccg
     3241 gacatgtcca acacagctgc agctacggct tctgggtcac tcagcatgtg caggtgcccg
     3301 cccggtaggt gagtcgtggg ccagccccgc tgcgccgcct gtgcagctcc cttgtcgtag
     3361 ggcttaccga accagatgta gccgaccgac ggtgcgctcc agtcatacgg cgcggacgga
     3421 agatggtcgt aataagccag cggcattcgc ggttgctcgg tttcgacctc ggcccgcacg
     3481 tatgcatccg ggaacaatgc atcaatgtcg gcctcgtccc accacgacgt ccacggcggc
     3541 agcaatccat ccacgatggc gagcctgccc agaaagtcgg atgtcgtgtg gtggccggca
     3601 cccggcagcg cagcgtcgat gaacacgaca ccgcgaacct gctcgccaag cgcctcgacg
     3661 accgccggca cgtacagtcc ggcgttgctg tgcgggacga ggacgaccgg ttcgtccgca
     3721 gccgctcgga tgatcgagtc gactcccgcg ggccagtacg gcggggtgct ttgtgcgacc
     3781 gccagcaagc tgggcacgtg cgcgcgccga tccttagccc gcagttcgcg ggcggtcgac
     3841 tgccaggtcg ccggtccgag aaatggactg tggatcagga tctgcgacgg ccccatgcga
     3901 tcagcatgcc gcggcgcacg tgagaagcgg cgcacgactc gcgtctcggc cgttcagccg
     3961 cgcgacgtcc gctcgtagcg ccgatgacgg ttgttgcggg cggcggtcgc gagcatggtg
     4021 agaccgactc cgagcatcgc ggcgatcgtc ggccattgga agggagcggg gagatacgtc
     4081 gcgaggaacc acgagcgagt cgatacgccg gtgagtttga agatgagggg gaccgcgcct
     4141 tggggtacca gcggaatcca gcacaacgac caacagagcg cgccgatggt gttgccggtc
     4201 gacgtgtggt gcgctgcggc gctctcgcgc accatattca gggtgagccg gacgcctccg
     4261 tgaccgcttt cgatcgtgtc gtggagcagt tgttctaggt cctgcccgta gcgtgctcgc
     4321 cattgcgcgg gataccacga caggaagcgc tcgatgcgcc gcgctgtcct ggccgccgga
     4381 ctcttgtcca agccccggta gcggcggatg gccgcgacga ccagccagcc aacgccaacg
     4441 ggccacagca gcacccagaa caccacggac aggacgacga aacgccacaa gccccatcgt
     4501 tgatacagcc gttcgacgcc ggtcacgaac tcaccgttgt gggtggactc cagctgacgg
     4561 ctagccttcg aaggccgaac tgtgcggtct gtgcttgcgc agtgagatgg gcatgcaaag
     4621 ccgcagcgcc gtcgccggtc aggcggtagg gcagccggcg gccgtcggcc tcaagggact
     4681 cgatgaggcc ctgtttctcg agccgggtca gcgccccgta caacgttccg ggctgcagcc
     4741 gcaccccggc gaacccttcg atatccgcgg tcagcgcata gccgtgtttc ggtccgccgg
     4801 ccaggctcga caagatcaac gtcgcaggac ccgcgtaatg gccgagcgcg ccaagaccgt
     4861 tctcgctgcg tgacatgccg ggaggctagt gcggttagcg gtataacgtc aagcgtccta
     4921 gcggaccttc cgcgcgatgg tgtgtgatcg cggtgtggag attgcgttgg gcaagccggg
     4981 agtcgatggg ctgagcgagg ctgtgggcgt gctgcgcgag tggcagtgcg acgaagcgcc
     5041 gatgcaattg catccggggg acctgggctg gttctggcgg ttcggtgcag aggcgacggc
     5101 cgcggcggtc cggacgtgga gccgggacgg acagattctc gccgtcgggc tgttggacgg
     5161 tcccacgctg ttgcggctga cggtcgcgcc aggcgctgag cgggacgagg agttggcgca
     5221 acagctggtc aacgacgtga ccgacccggc gcgcggcgta ctgccagcgg ggaaggtgtc
     5281 cgtcgaggcg ccaatggggg cgctggtaca agatctgctg ttcgaggacg gctggaacgc
     5341 cgacgagccg
//
LOCUS       DATCBJ010000375         4789 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_335493, whole genome shotgun sequence.
ACCESSION   DATCBJ010000375 DATCBJ010000000
VERSION     DATCBJ010000375.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 4789)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4789)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4789
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_335493"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(975..1988)
                     /locus_tag="VII50_07475"
     CDS             complement(975..1988)
                     /locus_tag="VII50_07475"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase"
                     /protein_id="HEY5050726.1"
                     /translation="MKAAVVTDFHSPLEIQDLPIPEPGPGEVLVRIETSGLCHTDIHA
                     AHGDWPVKPAPPFIPGHEGIGIIEKLGAGVRTRAVGDRVAIAWLGYACGECRYCIGGW
                     ETLCLKQENSGYSVNGTFAEYALAPAAFATPVPDAISSRDGAPLTCAGVTTFKAIKVA
                     GVVPGETVAIFGVGGLGHLALQYARIAGGFVIGVDIEDTKLAMATELGADYVINSRTT
                     DPVAGIEALGGADVAVALAASPQVFDQAYRSLRRGGRLVCVALPADNASLTLPIFDTV
                     INGKTVIGSIVGTRNDLADVFALHAAGRTKVIAVDRKIDEVNQAIDDVLAGRIPARVV
                     FQF"
     gene            complement(2424..2783)
                     /locus_tag="VII50_07480"
     CDS             complement(2424..2783)
                     /locus_tag="VII50_07480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009083475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF779 domain-containing protein"
                     /protein_id="HEY5050727.1"
                     /translation="MTSEAAEFLRRLTAQHGPLMFHQSGGCCDGSSPMCFPDGDFITG
                     DSDVHLGDLPIEGVERSIPVWMSVSQFAYWKHTHLTIDVVSGRGAGFSVEAPEGVRFL
                     IRSRLLTDDESAAFGLL"
     gene            complement(2821..4344)
                     /locus_tag="VII50_07485"
     CDS             complement(2821..4344)
                     /locus_tag="VII50_07485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009083477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEY5050728.1"
                     /translation="MTIYTPPGQSGSPVTVESRYDNFIGGDWVAPSKGDYFENPSPIT
                     GQTFTEIARSNAEDVDRALDAAHSARRAWGKTSATDRANILNKIADRMEQNLEMLAIA
                     ECWENGKPVRETLGADLPLAIDHFRYFAGAIRAQEGGISEIDGDTVAYHIHEPLGVVG
                     QIIPWNFPLLMAIWKLAPALAAGNCVVLKPAEQTPWSILKLMELVGDLLPAGVLNIVN
                     GFGVEAGKPLASSSRIAKIAFTGETTTGRLIMQYASQNLIPVTLELGGKSPNIFFEDV
                     ASARDDFYDKALEGFAMFAFNQGEVCTCPSRALIQRSIYDDFLGDAVQRTKKMRQGNP
                     LDTETQVGAQASNDQLEKILSYIDIGKKEGAKVLTGGVRASMEGDLAGGYYVEPTVFE
                     GKNSMRIFQEEIFGPVVSVTSFEDEADALSIANDTLYGLGAGLWTRDGSRAYRVGREI
                     EAGRVWTNCFHHYPAHAAFGGYKQSGVGRENHKMMLDHYQNTKNLLVSYSPKADGFF"
     gene            complement(4453..>4789)
                     /locus_tag="VII50_07490"
     CDS             complement(4453..>4789)
                     /locus_tag="VII50_07490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5050729.1"
                     /translation="MVETDCDWVGVAAHVAAGRVREALKAYHGPLLPQSEAPGIVRRR
                     DVLQAQLRGAVVASGHADLMAAWTRSRWGSNDLEMWQHEVHFLPANSPLRPAAEAEAR
                     RLEIEFGTV"
BASE COUNT          866 a         1560 c         1516 g          847 t
ORIGIN      
        1 gttcccggcg cgggagcttg gacagccacg ccgatcacca gagccatccg gtttgtcgcc
       61 gggtttcagc tgcctcacgg tcacctcgcc cacagtgcgc cggcgccgca gcgaaccaaa
      121 gaggctttgt acccggtgga cagggatcaa agacgactta cccccacccg agcgggacgc
      181 gacccccgca ctgccgcagc tcggagcagg aaggcccggc cgagcaaatt acgcgccctc
      241 aagatgcgtg caggatgcgt gcgaactccc tgtcacagat caccaactgc cggacaaccc
      301 aacaccagat aacggcgctg acctggcaga acaccactga ccgccgcccg acaacgctgg
      361 ccatcacgga ccaggttctc ctcttaatct gcgggttcgg ggttcaagtc cctggcggcg
      421 caccatgcct gcgcaccatg cctgacctgg ccttttacac ttcctggccc acctgcccga
      481 ggcacgccgt aggcccaaaa cgggcgaatg cgtgcaagaa gcgagtagcg ctcgcacgca
      541 tcgcggcagc ggtccgaaat ctgcgggtac gaagccaggc gcgcggggtg caacatggtg
      601 ccgccccttg ggacgtctca cggcggcacg caccgattga gcacccgcag acttcatcga
      661 cctctggcat gcccgactag cccgcgctcc ttcatccgcg agcaggcccg aaactcgtac
      721 caccgccatc cggaccgcat tagtcagcca cgcccccgac gactgtccac gattcgatcg
      781 gtcacgagct gacgatgacg ctgcatcctg ttccgtgaaa gacgatcgaa ccaaatgtcg
      841 acgacatcga tctgtcactc acacgcgcgg cgccacaacc agttattgcg caactccccg
      901 gttacctcag aagcgatccc gccggcgtat gagggtctgg aaccggaagc agccaacccg
      961 tacccgccgc ggggtcagaa ctgaaagacg acgcgggcgg gtatccgtcc ggcaaggaca
     1021 tcgtcgatgg cctggtttac ttcgtcgatt ttccggtcta ccgcgatgac cttggtgcgc
     1081 ccggccgcat gtagggcgaa gacgtccgcg aggtcgttgc gcgttccgac gatagaaccg
     1141 atgacggtct tgccgttgat gacggtgtca aagataggaa gggtgagcga cgcgttgtct
     1201 gcaggcaacg cgacgcacac cagtcgtccg ccacggcgca gtgagcggta ggcctggtca
     1261 aagacttgcg gcgatgcagc gagtgcgacc gcaacatctg cgccaccgag cgcctcaatg
     1321 cccgcgaccg gatcggttgt gcgtgagttg atcacgtaat ccgctccgag ttcggtcgcc
     1381 atcgccagtt tggtgtcctc gatgtcgacg ccaatcacga aaccaccggc gatgcgggcg
     1441 tattgcagcg cgaggtggcc taggccgccg acgccgaaga tcgccaccgt ctcccctggc
     1501 acgactccgg ccaccttgat cgccttgaac gtcgtcacac ccgcgcatgt cagcggcgca
     1561 ccgtcacggg agctgatcgc atccggcacg ggtgtcgcga acgcagccgg cgcgagcgca
     1621 tactcggcga aggttccgtt gacggagtag ccggagtttt cctgcttcag gcaaagcgtc
     1681 tcccacccgc cgatgcagta tcggcattcg ccgcatgcgt agccgagcca ggcgatcgca
     1741 acccggtccc cgaccgcacg ggtgcgcacg cccgcaccta gcttttcgat gatcccgatc
     1801 ccttcgtgcc cgggaatgaa cggcggggcc ggcttgacgg gccagtcgcc gtgcgcggcg
     1861 tgaatgtcgg tgtgacacag cccgctcgtc tcgatccgga ccagaacctc tcctggtccg
     1921 ggctcgggga tcggaagatc ctgaatctcc agcggggagt ggaagtcagt gacaacggcg
     1981 gccttcatag cgcgctccat tcagtcggac gaaacgagga ctcaagtaat tggtgtgccg
     2041 ttcccggcgc gggagcttgg gacagccacg tcgatcacca cagccatccg gtttgtctcc
     2101 aggtttcggc tgtctcacag tcacctagcc cacggtgcgc cggcgccgca gcgaaccaaa
     2161 gaggctttgt acccggtggg cagggctcaa agaaccgcgg cggtaggcga agaccgcgcc
     2221 ggagaactag cgacgagacc gtcctcgtcg acggcgatgg tcggttcctt acagcggcga
     2281 gagtcccgat ggtgctaatg gtcatggtgt tctccggtgt gggtaggctc gttctcattc
     2341 cccgccgggc gggagaggct aggtgcgctg tggtcgcgcc cagcgcgaac gccggatccc
     2401 ggagacttcc gacgcgcgcg atgttagagc aggccgaagg cggccgactc gtcgtccgtg
     2461 agaaggcggg accggatgag gaaccgcaca ccttcggggg cttcgacaga aaacccggcc
     2521 ccgcgccccg aaacgacgtc gatcgtcagg tgggtgtgct tccagtacgc gaattgactg
     2581 acggacatcc agaccgggat cgagcgctcc accccctcga tcggcaggtc gccaagatgg
     2641 acgtccgaat cgccggtgat gaaatctccg tcgggaaagc acatgggcga cgatccgtcg
     2701 cagcagcctc ccgattgatg gaacatcaaa ggcccgtgtt gcgccgtgag gcggcggagg
     2761 aattccgccg cctcactcgt cacgtcaact cgtgaatagc cggtggctgg ggtcatcggt
     2821 ttagaagaac ccatcggcct tcggcgagta cgacacgagc aggttcttgg tgttctggta
     2881 gtggtcgagc atcatcttgt ggttctcgcg cccgactccc gactgcttgt acccgccgaa
     2941 cgcagcgtgc gccgggtagt ggtggaagca gttcgtccag acccggccgg cttcgatctc
     3001 gcgaccgacg cggtaggcgc gcgatccgtc ccgggtccac aagcccgcgc caagaccgta
     3061 gagggtgtca ttcgcgatgc ttaacgcgtc tgcctcgtcc tcgaagctgg tgaccgacac
     3121 gaccgggccg aagatttcct cctggaagat tcgcatcgag ttcttgcctt cgaagaccgt
     3181 cggctcgacg tagtagccgc cggctaggtc accctccatc gaagctcgga cgccgccggt
     3241 gagcaccttg gcgccttcct tcttgccaat gtcgatgtag gagaggattt tctccaactg
     3301 gtcgttggat gcctgagctc cgacctgcgt ctcggtgtcg agcgggttgc cctgccgcat
     3361 cttctttgtc cgctgcactg cgtcgccgag gaaatcgtcg tagatggagc gctggatcag
     3421 cgcgcgcgac gggcaggtgc agacctcgcc ctggttgaac gcgaacatcg cgaagccttc
     3481 gagggccttg tcgtagaagt cgtcgcgcgc agatgcgacg tcctcgaaga agatgttcgg
     3541 gctcttgccg ccgagttcca acgtcaccgg tatgaggttc tgcgaggcgt actgcatgat
     3601 caatcgccca gtcgtcgtct cgccggtgaa ggcgatcttg gcgatcctgc tggacgacgc
     3661 gagcggcttg ccggcttcca cgccaaaccc gttgacgatg ttgagcacac cagcgggcag
     3721 taggtccccg acaagttcca tcagcttcag aatcgaccac ggtgtctgct cagcgggctt
     3781 gagcaccacg cagttaccgg cggccagcgc cggcgcaagc ttccagatcg ccatgagcag
     3841 ggggaagttc caggggatga tctgcccgac gacgcccagc ggctcatgga tgtgatacgc
     3901 cacggtgtcg ccgtcaatct cggagatgcc accctcttgg gcccggatgg cgccggcgaa
     3961 atagcggaaa tggtcgatgg caagcggtag gtccgcacca agagtttccc gtaccggctt
     4021 gccgttctcc cagcactcgg cgatcgcgag catctccagg ttctgttcca tccggtccgc
     4081 gatcttgttt aggatgttcg cgcggtcggt tgcggatgtc ttgccccacg cgcggcgcgc
     4141 gctgtgggcg gcgtccaggg cgcgatcgac gtcctcggcg ttggagcggg cgatctcggt
     4201 gaaggtctgc ccggtgatgg gcgatggatt ctcgaagtag tcgcccttgc tgggcgccac
     4261 ccaatcgcca ccgatgaagt tgtcgtagcg ggactcgacg gtgaccgggc tgcccgactg
     4321 tccgggtggg gtgtagatcg tcattgatct cctttgctgc actagcgtcc gaggacctca
     4381 cgagggccct gctccgcacg ctaggccgca caaggttgca accacgttgc aaccgatccc
     4441 ggaatcgtcg cttcagacgg tgccgaactc gatttcgagg cgacgggctt cggcctccgc
     4501 ggccggtcgg agcggggagt tcgcaggcag gaaatgaacc tcgtgctgcc acatctcgag
     4561 gtcattggaa ccccaccgcg atctggtcca cgccgccatc aggtcggcgt ggcccgatgc
     4621 aacaacagcg ccccgtagct gggcctggag aacgtctcgg cggcggacga tgcctggcgc
     4681 ctccgattgg gggagaagcg gtccgtggta cgccttgagc gcctcgcgaa cccgccccgc
     4741 ggccacatgg gccgcgaccc cgacccagtc gcagtcggtc tcgaccatc
//
LOCUS       DATCBJ010000376         2158 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_440087, whole genome shotgun sequence.
ACCESSION   DATCBJ010000376 DATCBJ010000000
VERSION     DATCBJ010000376.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2158)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2158)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2158
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_440087"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1335
                     /locus_tag="VII50_07495"
     CDS             <1..1335
                     /locus_tag="VII50_07495"
                     /inference="COORDINATES: protein motif:HMM:NF013918.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin reductase family protein"
                     /protein_id="HEY5050730.1"
                     /translation="AAAARRTVGLTVLWLALLANASVIVWLWYHGGNVTGVHSQGQLL
                     TSIARITGLLGAYLALVQVILLARVRPLERLVGFDRLTVWHRWNGYATLWLILAHVFF
                     SIWGYADMDRLSFVNEIPTMIWQGHYPGMIVATVGTFLFVVVVASSVVIVRRRLDYRY
                     WYAVHFTVYAAIALGWFHQIPTGNELVVDTAAADYWRSLYLATLALLLAFRLVIPAAN
                     ALRYRLRVAEVVPEGPGVVSLRITGRDLDHLKARGGQFFLWRFLTRRDWFDSHPFSLS
                     ALPEDGSLRITVKSLGDFTSRAGDLAPGTRVMAEGPFGVFTTATRRRDKVALIAGGIG
                     ITAVRALLQEADGDVVVLYRALTDQDLVLRREVDALARPSGITVHYIVGDHATPDGER
                     LLAPEHLTELVPDIREREIYVCGPPGMTDVTAKNVRAAGVPRRHIHIERFAL"
     gene            1350..1781
                     /locus_tag="VII50_07500"
     CDS             1350..1781
                     /locus_tag="VII50_07500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5050731.1"
                     /translation="MYKIVTTVVGAAALGLTPVGAAAAQTMKRQAKAVVTTTLVRGPQ
                     EAAGPWGNLQVTLIVKKATTTSGKRKTIVRTITNLSVPIYPNHTARSVYINSVALPLL
                     KQEVLKAHFNLSKVYLISGASYTSIAYGQSLQAALISAGKV"
     gene            1781..>2158
                     /locus_tag="VII50_07505"
     CDS             1781..>2158
                     /locus_tag="VII50_07505"
                     /inference="COORDINATES: protein motif:HMM:NF014477.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HEY5050732.1"
                     /translation="MPSKLPGTRRIEQVMGMPIMVDLRGEESDDDALLDEIFEWFRRV
                     DAKFSTYRDDSEVSLIRRGELEADDASAEVREVLARCEQLSAETDGYFDAWACSPDGL
                     DPSGLVKGWAVDGAVATLARAGRR"
BASE COUNT          361 a          729 c          684 g          384 t
ORIGIN      
        1 gccgccgcag cacgccgcac cgttggcctg accgtccttt ggctcgccct gctcgcgaac
       61 gcttcggtga tcgtctggct ttggtaccac ggcggcaacg tcaccggtgt gcactcgcag
      121 ggccagctgc tgacgagcat cgcccgcatc accggactgc tcggcgccta cctggcgctc
      181 gtgcaggtca tcctgctcgc acgcgtgcgg ccgctcgagc ggctcgtcgg ctttgaccgg
      241 ctcaccgtct ggcaccgctg gaatggctac gccactctgt ggctgatcct tgcgcacgtc
      301 ttcttctcga tctggggcta cgccgacatg gaccggttga gtttcgtgaa cgagatcccg
      361 acgatgatct ggcaaggcca ctaccccggg atgatcgtgg caacggtcgg gacgttcctg
      421 ttcgtggtcg tagtcgcgag ctcggtggtg atcgtccggc ggcggctcga ctaccgctac
      481 tggtatgcgg tccacttcac cgtctacgcc gcgatcgcgc tcggctggtt tcaccagatt
      541 ccgaccggca acgagctcgt cgttgacacc gccgccgccg actactggcg cagtctctac
      601 ctggcgaccc tcgcgctgct tctggccttc cgcctggtca ttccagcagc gaacgcgctg
      661 cgttatcggc tgcgcgtcgc ggaggttgtg ccggagggtc ccggcgtcgt ctcgcttcgg
      721 atcacaggcc gcgacctcga ccacctcaaa gcacgcggcg gccagttctt tctgtggcgc
      781 ttcctcaccc gccgcgactg gttcgactcc catccctttt cgttgtctgc cttgccagaa
      841 gacgggtcgc tgcgcataac ggtgaagagc ctcggcgact tcaccagtcg cgccggtgac
      901 ctcgcgccgg ggacgcgcgt gatggccgaa ggaccgttcg gcgttttcac gaccgctaca
      961 cgccggcgcg acaaggtcgc gttgatcgcc ggtggcatcg ggatcacagc cgtgcgagca
     1021 ctgttgcagg aggcggacgg cgatgtcgtc gtcctctacc gcgcactcac tgaccaggac
     1081 ctcgtcctcc ggcgggaagt cgatgcgctc gcgcgcccaa gtgggatcac cgtccactac
     1141 atcgttggcg accacgccac gcctgacggc gaacggctgc tcgcgccaga gcacctgacc
     1201 gagctcgtgc ccgacatccg cgagcgcgaa atctacgtgt gcggaccccc cggcatgacg
     1261 gatgtcaccg ccaagaatgt ccgtgcagcc ggtgtacctc gccggcacat ccacatcgaa
     1321 cgctttgcac tctgagaaag gaccacgata tgtacaaaat cgtcacgacc gttgtaggcg
     1381 ccgcggcgct cgggctaacg cccgtcggcg ccgcggcggc ccaaacgatg aagcgccagg
     1441 caaaggcagt tgtcacgacg acactggtga gagggccgca ggaggccgca ggtccctggg
     1501 gcaacctcca ggtgaccctg atcgtgaaga aggcgaccac gaccagcggc aagcgaaaga
     1561 cgatcgtgcg cacaatcacg aatttgtcgg tccctatcta tcccaaccac accgcccgct
     1621 cggtgtacat caactcggtg gcacttcccc tgctgaagca ggaggtgctc aaggcccact
     1681 tcaacctgag caaggtctat ctgatatcag gcgccagcta cacaagtatc gcgtacgggc
     1741 agtcgctgca ggctgccctc atcagcgctg ggaaggtctg atgccaagca agctgcctgg
     1801 cacgcgccgc atcgagcagg tgatgggtat gccgatcatg gttgacctgc gcggggagga
     1861 aagcgacgac gacgcgctgc tggatgagat cttcgagtgg tttcgccgcg tcgacgcgaa
     1921 attcagcacc tatcgagacg acagcgaggt cagtctcatc cggcgcggcg agctcgaagc
     1981 ggacgatgcc agcgcggagg tgcgagaagt gctcgcgcgc tgcgagcagc tctccgccga
     2041 gaccgacggc tacttcgacg cctgggcgtg ctcgccggat gggctcgatc catcgggcct
     2101 cgtcaagggc tgggcggtcg acggcgcggt ggcgaccctc gcccgcgccg gtcggcgc
//
LOCUS       DATCBJ010000377         3172 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_520283, whole genome shotgun sequence.
ACCESSION   DATCBJ010000377 DATCBJ010000000
VERSION     DATCBJ010000377.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 3172)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3172)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3172
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_520283"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            188..442
                     /gene="rpsT"
                     /locus_tag="VII50_07510"
     CDS             188..442
                     /gene="rpsT"
                     /locus_tag="VII50_07510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009648032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HEY5050733.1"
                     /translation="MANIKSQIKRIGTNEKARLRNKAVKSSLKTHVRRYREAVAAGSP
                     DAPELLRAATRQLDKAASKGVIHANQAANRKSALAKQASV"
     gene            complement(453..1421)
                     /locus_tag="VII50_07515"
     CDS             complement(453..1421)
                     /locus_tag="VII50_07515"
                     /inference="COORDINATES: protein motif:HMM:NF044544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HEY5050734.1"
                     /translation="MSADRRTSSVTVVVGDEELLVERAIAAVVSVARLADADTDVRDL
                     AGALLEPGQLAQMTSPSLFGERHVLVVRDVEQLPKEVVDEVIASCRDLADEVVIVLVH
                     KGGARGKPLLDAASGLGAERVECAKLTRPGDRVDFVRAEFRNAGRVVSENGARALIDA
                     VGNDLREIAAACAQLCTDTDGQVDGELVARYYRGRAEATGFAVSDRALEGRLGEALEQ
                     LRWALAVGVAPVLITSALAQGIRALAKVASAPRGLRGAELARELEMPPWKVDRVRAQL
                     RGWTPTGVATAVTAVAQADEQVKGGGTDAGYALEKAVLAIVAARAA"
     gene            1706..>3172
                     /locus_tag="VII50_07520"
     CDS             1706..>3172
                     /locus_tag="VII50_07520"
                     /inference="COORDINATES: protein motif:HMM:NF024594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4012 domain-containing protein"
                     /protein_id="HEY5050735.1"
                     /translation="MRVALVLVLMLVAAAAWVVYSGLRAKDNLLRAAHDVAVLQTQAE
                     SGDVAGERISLASLQREARSARVETGGPIWRLARWTPVIGSNAAAITTVASAVDDLAQ
                     QALPSIVDTSATLDLRTIAPHDGRFNLAPIQHAEPAILAAASVVQRVRDQVAGISTSR
                     LVPLVRTSVLRLRSELDAAGTTTSAAARAVTLLPPMLGASQPRTYPLLFQNNAEVRAT
                     GGIAGSWAIVRVEHGAVHIVKQGSAGDVNSHLSGVPVPPDVAALYTNRPGTFFQDVDM
                     APSFPTDATLVSEMFEQAYGIAVDGVISTDPVALSALLKATGPVQLPDGRSLTASNAV
                     SLLLSQVYQDIQDPAQEDIFFAQAANAVFTALSAGQGGARAMVEALAAAAGAGRLTVW
                     SRHPDEQQLLAGTELGGAMPVTEQPANPTIGVFLNDGTGAKLDYYLHEDVAVMSAATC
                     PADRIGFHVLVTLTSTVPANAKGLPAYVTGDPRAGVALG"
BASE COUNT          520 a         1133 c         1021 g          498 t
ORIGIN      
        1 cctgaacgtg atcttgtaca aatgggccag ccgcaccggt ggagtccaga taccgcgcga
       61 gagcggtctc ggccagcctg gttggacgcc gaacctgccg cttgggtact ctgagtggtc
      121 gcacctgcgg atttgcagct agcgcagttg tgcattctga ccccggacac tcgatgaggc
      181 ttcccgcgtg gcgaatatca agtcccagat caagcgcatc ggcacgaacg agaaggcacg
      241 gctgcgcaac aaggccgtga agtcctcgct caagacccat gtgcgccgtt accgcgaagc
      301 ggtcgcggcc gggtcacccg acgcgcctga gttgctgcgc gccgcgaccc gtcagctcga
      361 caaggccgcc agcaagggcg tcatccacgc caaccaggcc gcgaaccgca agtcagcgct
      421 ggcgaagcag gcctcggtct aagaacggca cgtcaggcgg cgcgagccgc cacgatcgcc
      481 aacaccgcct tctccagcgc gtagccggca tcggtgccac ctcccttgac ctgctcatcg
      541 gcctgcgcga cggccgtcac ggcggttgcc acgccggtcg gcgtccatcc ccgcagttga
      601 gctcgcaccc gatcgacctt ccacggcggc atctcgagtt cgcgggccag ttccgcgccg
      661 cgcagccccc gtggcgcgct ggcgactttg gccagtgccc tgatcccttg cgcgagcgcg
      721 ctggtgatca acaccggcgc aaccccgacc gccagcgccc agcgcagctg ctccagtgcc
      781 tcgccaagcc ggccctccag cgctcggtcg gagaccgcga agccggtcgc ctcggcgcga
      841 cctcggtaat agcgcgccac gagttcgcca tcgacctggc cgtcggtgtc cgtgcacagc
      901 tgagcgcacg ctgccgcaat ctcgcgcaag tcgttgccga cagcgtcgat caaggccctc
      961 gcgccgtttt cgctgacgac ccggcccgca ttcctgaact ccgctcggac gaagtcgacg
     1021 cggtcccccg gcctggtgag cttcgcgcac tcgacccgct cggcccccag cccgctggcc
     1081 gcgtcgagca acggcttgcc gcgcgcgcca cctttgtgga cgagcacgat cacgacctcg
     1141 tcggcgaggt cacgacatga cgcgatcacc tcgtcgacca cttctttggg cagttgttcg
     1201 acgtcccgca cgaccaggac gtgtcgctcg ccgaacaacg aagggctcgt catctgggcc
     1261 agctggccgg gttcgagcaa ggcgccggct aggtcgcgca cgtccgtgtc agcgtcggca
     1321 agccgcgcga cggagacgac cgcggcgatc gcgcgttcga cgagcagttc ttcgtcgcca
     1381 acaaccacgg taaccgagct ggtacggcgg tcagcagaca cgacaggcag catgccatgt
     1441 caccgacctc cggtgtcgac ctcctgcacg ggctcaggct ctggtgcgaa acgccgagaa
     1501 tcaccccgat gatcagcgct gaccgggccc ggccgggggc gacccctgcc gtctgcgccg
     1561 gcaagttgtg ccgctccccc gtggtggagc ggctcttccg cgtgcggtcg cgatgaccgc
     1621 gctgctgagc gcgccccacg ttgcgaccca ccgcgggcca aggcacgcca cgcctcgtcg
     1681 caggggcagg tcgagacgac ggctcttgcg cgtcgcgctg gttctcgtgc tgatgctcgt
     1741 cgccgcggcc gcgtgggtcg tctacagcgg ccttcgagcg aaagacaacc tgctgcgcgc
     1801 agcccacgac gtcgcggtgc tgcaaaccca agcggagagc ggcgacgtcg cgggcgaacg
     1861 aatcagtctg gcaagcctgc agcgggaagc acgctcagca cgagtcgaga ccggcggccc
     1921 gatctggcgg ctcgcgcgat ggacgcccgt catcggaagc aacgccgcgg cgatcacgac
     1981 ggtcgcgtcg gcagtcgatg accttgctca gcaggcgttg ccctcaatcg tcgacacgtc
     2041 ggcaacgctt gacttgcgga caatcgcgcc ccacgacggg cggttcaatc ttgcgccaat
     2101 ccagcacgcg gagccggcaa tcctcgctgc agcgtcggtg gtccaacgag tgcgtgacca
     2161 agtggccggc atctcgacgt cccgcctcgt gcctctcgtg cgcacgtcgg tgctgcggct
     2221 acggtcagaa ctcgacgcgg cggggaccac gacctcggcg gcggcgcgcg cggtgacgct
     2281 cttgcccccg atgctcggcg cctcccagcc gcggacgtac cccctgctgt tccagaacaa
     2341 cgccgaggtg cgtgcgactg gtgggatcgc gggttcgtgg gccatcgtgc gcgtcgagca
     2401 cggcgccgtc cacatcgtga aacagggatc cgcgggcgac gtcaacagcc atctctccgg
     2461 agtgcccgtg ccacccgatg tcgcggcgct ctacacgaat cgaccaggca cgttctttca
     2521 ggacgtcgat atggcgccgt cgttccccac cgacgcgacc cttgtcagtg agatgttcga
     2581 acaggcctac gggattgcag tggacggcgt catctccaca gaccccgtgg cgctatctgc
     2641 gctgctgaag gcgaccgggc cggtgcagct gccagatggc cgatcgctga ctgccagcaa
     2701 cgccgtgtct ttactgctgt cgcaggtgta ccaggacatc caggacccag cacaggagga
     2761 catcttcttc gcgcaggccg cgaacgccgt ctttactgcg ttgtcggctg ggcaaggcgg
     2821 cgcgcgcgcg atggtcgaag ctttggcggc cgccgcaggt gcaggccgtc tcaccgtgtg
     2881 gagtcgccat cccgatgaac agcagctgct tgcgggcacc gaactcggcg gcgcgatgcc
     2941 ggtgactgag caacccgcaa accccaccat cggcgtgttt ctcaatgacg gaaccggcgc
     3001 caagctcgac tactacctgc atgaggacgt ggccgtcatg tcggccgcga cctgccctgc
     3061 cgaccggatc ggattccacg ttctcgtcac tctgacgtcg acagttcccg cgaacgcgaa
     3121 gggactaccg gcatacgtga ccggggatcc acgagctggt gtcgcgctcg gg
//
LOCUS       DATCBJ010000378         3712 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_472299, whole genome shotgun sequence.
ACCESSION   DATCBJ010000378 DATCBJ010000000
VERSION     DATCBJ010000378.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 3712)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3712)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3712
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_472299"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..711)
                     /locus_tag="VII50_07525"
     CDS             complement(<1..711)
                     /locus_tag="VII50_07525"
                     /EC_number="4.2.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009073319.1"
                     /note="catalyzes the formation of
                     4-imidazolone-5-propanoate from urocanate during histidine
                     metabolism; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urocanate hydratase"
                     /protein_id="HEY5050736.1"
                     /translation="MSGPRPVRAPHGNTLSCQGWPQEAAMRMLMNNLDPAVAERPDDL
                     VVYGGTGKAARDWNSFDAIVRTLQGLKDDETMLVQSGRPVGVMRTHEWAPRVLIANSN
                     LVGDWATWPEFRRLEHLGLTMYGQMTAGSWIYIGTQGILQGTYETFAAVAAKRFGGTL
                     AGTLTLTGGCGGMGGAQPLAVTMNGGAVIVVDVDPGRLERRVEQRYLDEWTADIDVAL
                     DRALAAKREGRALSVGLVG"
     gene            complement(708..2261)
                     /gene="hutH"
                     /locus_tag="VII50_07530"
     CDS             complement(708..2261)
                     /gene="hutH"
                     /locus_tag="VII50_07530"
                     /EC_number="4.3.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF006871.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004397 - histidine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ammonia-lyase"
                     /protein_id="HEY5050737.1"
                     /translation="MTQARDVTVVAVGQPPLEPTQVVAVARDGARVEVGASALARVAA
                     SRAHIEALAESGEPVYGVSTGFGALATRHIAPDLRAQLQRSLVRSHAAGSGVEVEREV
                     VRAMMLLRLATLATGRTGVRPLIATGYAAMLNAGVTPVVREFGSLGCSGDLAPLAHCA
                     LALMGEGEVRDAAGTLMPAAAALQAVGIQPVVLGAKEGLALINGTDGMLGMLLLACHD
                     LSVLAKNADIAAAMSVEAILGTDAVFAADLQALRPHPGQAASAANLRALLAGSPIVES
                     HRVDCTLVQDAYSLRCAPQVAGAFRDTLAHALAVAGRELASAIDNPVVLPDGRVESNG
                     NFHGAPLGYVLDFLAIVAADLASMSERRSDRLLDVARSHGLPAFLAVDPGVDSGHMIA
                     QYTQAAIVSELKRLAVPASVDSIPSSAMQEDHVSMGWSAGRKLLRSVDGLTRVIAIEI
                     LTAARALDLRAPLRPAPGTAAVVAALRTRVAGPDVDRWLSPEIETAVDMTRAGSFVHA
                     AEAVIGALS"
     gene            2732..>3712
                     /locus_tag="VII50_07535"
     CDS             2732..>3712
                     /locus_tag="VII50_07535"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HEY5050738.1"
                     /translation="MPARVVVLTGVAGYLGALATAVWELLKGAPLHAPAYHQLAVAGT
                     FALAAVASTEISMRFAWPRTRRDRVSRDLMAVWLLPIALLLPPAYVMVSVIVLRGYLY
                     ARVWRAQLLKNVYTTAALGLAYGGASLVHRVVLHATMDTNVTASQFTGGSIAAVSLLL
                     AVLTYWAVNAVLIAAVVVMTAGADALRTFLKDREGLVVDVVAGCLGILAAVLWATEPM
                     LAVLLVPPVLLLEHQLFSGLRQAVRTDLLTDVANPQYWREVAGREVERAAASNDHLAI
                     LMIDVDHFKSVNDRYGHLAGDEVLAAVARTIAQALRPSDLVGRLGGEEFGA"
BASE COUNT          609 a         1297 c         1206 g          600 t
ORIGIN      
        1 accgactagt ccaacagaca gcgcacgccc ctcacgcttt gcggccagcg cgcgatccag
       61 cgcaacgtcg atgtcggcgg tccactcgtc gaggtagcgc tgctcgacac gccgctccag
      121 ccgcccaggg tcgacgtcga cgacgatcac cgcgccgccg ttcatcgtga ccgcgagcgg
      181 ttgcgcacca cccatcccgc cgcagccgcc ggtcaatgtc agcgtcccgg cgagcgtgcc
      241 accgaatcgc ttcgcagcaa ccgcagcgaa ggtctcgtag gtgccttgca ggatcccctg
      301 cgtgccgatg tagatccacg aaccggccgt catctggccg tacatcgtca gcccgaggtg
      361 ctcgagccgg cggaactccg gccatgtcgc ccaatcaccg accagattcg agttcgcgat
      421 gagcacccgc ggcgcccatt cgtgggtgcg catcacgccg acgggccggc ccgactgcac
      481 gagcatcgtc tcgtcgtcct tcaaaccctg cagggtgcgc acaatggcgt cgaaggagtt
      541 ccagtcgcgg gccgcctttc cggtcccgcc gtacacgaca agatcatcgg gacgttcggc
      601 aacggcagga tcgaggttgt tcatcagcat ccgcatggcg gcttcttgcg gccagccctg
      661 acacgacagg gtgttgccgt gtggtgcccg caccgggcga ggtccgctca cgagagtgct
      721 ccgatcacag cttcggcggc gtgcacaaag cttcccgcac gggtcatgtc cacggcggtc
      781 tcgatctccg gcgatagcca ccggtcgacg tccggccctg cgacgcgggt acgtaaggct
      841 gcaacgaccg ccgcggtgcc cggggccggc cgcagcggcg cgcgcaagtc cagagctctt
      901 gctgcggtga gaatttcgat cgcgatcacc cgggtcagac cgtcgacgga gcgcagcaac
      961 ttgcggccgg ctgaccagcc catcgagacg tgatcctcct gcatcgccga tgacggaatc
     1021 gagtcgacac tcgccggaac cgcaagtcgc ttcagctcac tgacgatcgc cgcttgcgtg
     1081 tactgcgcga tcatgtgccc cgagtcgact cccggatcga cggcgaggaa cgccggcaat
     1141 ccgtgcgacc gcgccacatc gagcagccgg tcgctgcgcc gctcggacat cgacgcgagg
     1201 tcggcggcca cgatcgccaa gaagtcgagc acatagccga gcggcgcacc gtgaaagttt
     1261 ccgttcgact cgacccgccc gtcgggcagt accaccgggt tgtcgatggc gctcgcgagc
     1321 tcgcgacccg cgacggccaa cgcatgcgcg agtgtgtcgc gaaacgcgcc ggcgacctgc
     1381 ggcgcacagc gcagcgaata ggcatcctga accagcgtgc aatcgacgcg atgcgactcg
     1441 acgatcggcg agccggccag cagcgcgcgc aggttcgctg ccgaggcggc ctggccaggg
     1501 tgcggccgca gggcctgcag gtcggcagcg aacaccgcat cggtgccaag gatcgcctcg
     1561 acactcatgg cggccgcgat gtcggcgttc ttcgccaaca ccgacaggtc gtggcacgcg
     1621 aggagcagca tgcccagcat cccgtcggtg ccgttgatca gtgcaagacc ttccttggcg
     1681 cccagcacga cgggttggat gccgacggcc tgcaaggcag cagccgcggg catcaacgtg
     1741 ccggcggcgt cgcgcacttc gccctcgccc attaacgcca atgcgcagtg cgcgagtggc
     1801 gcgaggtcac cggaacaacc cagcgagccg aactccctga ccaccggcgt cacaccggcg
     1861 ttcagcatgg ccgcgtagcc cgtggcgatc aacggtcgga cgccggtgcg accggtcgcc
     1921 agtgtcgcaa gacgcagcag catcatcgcg cgtacaactt cacgctcgac ctcgacgcca
     1981 ctaccggccg catgcgagcg cacgagcgag cgctgcaact gtgcgcgcag gtcaggcgcg
     2041 atatgtcggg tggccagcgc gccgaatccg gtcgagacgc cgtacaccgg ctcgccggac
     2101 tccgccagcg cctcgatgtg cgcccgcgag gcggcaaccc tggcgagcgc cgaggcgccc
     2161 acctcgaccc gcgcaccgtc gcgggcaacg gcgaccactt gtgttggctc gagtggcggc
     2221 tgtccgacag cgacgaccgt cacgtcgcgg gcctgcgtca tggggacagt gtgccgcgag
     2281 ctgtcgggtg gccggacgac ggcaggtcca tccggccacc cgagactctc accagatagt
     2341 cacttgtgat tcgtgagtcg tcgtgctgct cgtctaggtc caagaccaat cgcgcatggt
     2401 tgctcacctc cctcgcacgt gctgggcaag cgctgggcga gtgctgggca ggtgcttgtc
     2461 gacccgctga ccgaatcggc ccttaggccg ttgatcagcc ttgatccgcc aaacaagatc
     2521 acttgtgagc gatcggttct attctgtccc gattcttaac ccggacagcg gtgacacgac
     2581 ggcccagacc ccgtaaagtc cgcagcgcag agcgccctat tcctcgcctg ctgggcgtga
     2641 tgtgaggaga cccgtgtaag tgacgcgaaa ttggcgctgg ggcgcttcgc gccagagacg
     2701 ctgggccgca tggagcgtgt ggcaactgcc ggtgccagcc agagtcgtgg tgctgaccgg
     2761 cgttgccgga tatctcgggg ctctcgcgac ggctgtttgg gaactgctca aaggcgcccc
     2821 gctgcacgct cccgcctatc accagctcgc ggtcgccggc acgttcgccc ttgcggcggt
     2881 cgccagcacc gagatatcga tgcggtttgc ctggccgcgc actcggcggg accgcgtctc
     2941 gagggacctc atggcggtct ggctgttgcc gatcgcgttg ctgctgccgc ccgcgtacgt
     3001 gatggtcagt gtgatcgtct tgcgcgggta cctctacgcc cgggtctggc gggcacagct
     3061 gctcaagaac gtgtacacca ccgctgcgct cgggctcgct tacggtggcg cctcgctcgt
     3121 gcaccgggtc gtgctccacg caaccatgga caccaacgtc accgcgagcc agttcaccgg
     3181 gggcagtatc gccgccgttt cgctgcttct cgccgtcctg acctactggg cggtgaacgc
     3241 ggtcctgatc gctgccgtcg tcgtcatgac cgcaggcgca gacgccttgc gcacctttct
     3301 gaaggatcga gaaggcctcg tcgtcgacgt tgtcgccggc tgcctcggca tcctggctgc
     3361 cgtcttgtgg gccaccgaac cgatgctggc ggtcctgcta gtgccgcccg ttctgctgct
     3421 cgagcaccaa ctcttcagcg gacttcggca ggcggtacgc accgatctcc tgaccgacgt
     3481 cgcgaacccc cagtactggc gtgaagtcgc gggccgcgag gtcgaacgag cagcggccag
     3541 caatgaccac ctcgcgatct tgatgatcga cgtcgaccac ttcaagtccg tcaacgacag
     3601 gtacggccat ctcgcgggcg atgaggtgtt ggccgcagtc gcccgcacca tcgcgcaggc
     3661 cctgcgaccg agcgacctcg tcggccgatt gggcggggag gagttcggcg cg
//
LOCUS       DATCBJ010000379         4744 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_74461, whole genome shotgun sequence.
ACCESSION   DATCBJ010000379 DATCBJ010000000
VERSION     DATCBJ010000379.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 4744)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4744)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4744
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_74461"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..447
                     /locus_tag="VII50_07540"
     CDS             <1..447
                     /locus_tag="VII50_07540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="3-beta hydroxysteroid dehydrogenase"
                     /protein_id="HEY5050739.1"
                     /translation="FAPTVHGEGDNGFMAGLVGVARDKGVAGYIGDGTNRWAAIHRGD
                     AGRVVRLALEHALEPGTVLHAVAETGIATRDIAEAIGRGLHVPVHSIAPDEAWAHFGW
                     LSMFYGMDIAATSETTQAALAWKPDGPTLLDDLDAGSYFHGPDPTR"
     gene            939..2153
                     /locus_tag="VII50_07545"
     CDS             939..2153
                     /locus_tag="VII50_07545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5050740.1"
                     /translation="MTSRSDVDPGLERDAERGGVRTCFRWATRRTALVVGVATVLMFA
                     ASIPLATLSKAKNGSVVVLPFAVLGFVLARRQPRNPIGWIMLLLGPIVIVSGDAALYS
                     LLAYHVDGHDLPLSRLAVVLAPDWALLQVALPLPILLFPDGRIASRRLWWTLWAYVVV
                     GVAWLCDLAIRGSVAFTQHPVRIDPMGQSVNLDVTAGVFDDIQNVLAVAYVGLVLSWV
                     LRLLVAYRGSAGDLRQQLKWLMVGGALCVVGILSGALLSGIGSALFSLILAFPICIGI
                     AILKYRLYDIDRLISRTLSYLILTALLIGVYVGLVALTTRALPLSSPIGVAASTLTVA
                     ALFNPVRRRAQRLVDRRFNRARYDADATIAAFTAALRNEIDLDRVQHDLVNVVYRSVQ
                     PAHVSVWIRSSA"
     gene            3108..4337
                     /locus_tag="VII50_07550"
     CDS             3108..4337
                     /locus_tag="VII50_07550"
                     /inference="COORDINATES: protein motif:HMM:NF025019.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4143 domain-containing protein"
                     /protein_id="HEY5050741.1"
                     /translation="MIDSALQRALAAAGAVVLEGARATGKTTTALHAAASWVFIDDAQ
                     AQSLLDVAPRSLIEGDAPRLLDEWQVAPQLWNLVRRAVDATAEPGLFILTGSAVPPDD
                     VTRHTGGGRFLRLRERTMSWWEKLDSPTGAVSLAGLFEGNLPKTDLGAAPDLDTVIDN
                     LLRPGFPAMTALAAAQSADRLRGYIDDVARTDIRRIADIRHEPEVIKQLIAGLARSVA
                     SEVTYKTLAADVRVVAPTIDEETVSNYVGLLQRLFVVEAQRPWTPTLRSRARVRTSPK
                     FHLVDPALAAAALGAGAQHLRGDLNTLGTLFESAVVHDLTVLTLGGEVSHYRDSNGKE
                     IDAILTLPDGRWGAVEVKLGGQQVSMGARSLQAVIAQVDTAAIGEPTFRLVVTGTGPI
                     LVTDDGTVTAPLTALGP"
     gene            4385..4612
                     /locus_tag="VII50_07555"
     CDS             4385..4612
                     /locus_tag="VII50_07555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5050742.1"
                     /translation="MPKHTPARVTSDTPIGTDVDLDRDDIRLADGSRLTQEVANGVVE
                     HARRVAGRPSLSGNAEESPQIEFPVAPEHPR"
BASE COUNT          786 a         1626 c         1490 g          842 t
ORIGIN      
        1 ttcgccccga ctgtccacgg cgagggtgac aacggattca tggccggcct ggtcggcgtc
       61 gctcgcgaca agggtgtcgc gggctacatc ggtgacggta caaaccgatg ggccgcgatc
      121 caccgcggcg acgcgggcag ggtcgttcgg ctcgccttgg aacacgcact ggagccggga
      181 accgtgttgc atgcagtcgc cgagacaggc atcgcgactc gcgacatcgc cgaagctata
      241 ggccgcgggc tacacgtgcc cgtccactcc atcgccccag acgaagcatg ggctcacttc
      301 ggctggctca gcatgttcta cggcatggac atcgccgcaa ccagtgaaac cacccaggcg
      361 gcgctggcct ggaagccgga cgggccgacg ctgctcgacg atcttgatgc gggatcgtac
      421 ttccacggcc ctgacccgac gcgataacga ccggccacag cagcaccgac gtgcctacca
      481 aacccgcgcc gatgcgctcg gcaggacgat catcgaggtc gccccgcgtc cccttccacg
      541 ttgtcgcagc cgcgcttcgc ggcggcgcgc agatcgacgg cccaatcaga tagccagcac
      601 tacaccaaaa ccgtcacatc tcaaagaggc ccgccgagcg acggccgagc ccgtaaggac
      661 gactgcgatg ccgacactgc atggaccacg atcggcgatc tcgacccgcg aaaagaatac
      721 gtcgatgtag cgacacgctt cgtcttcact caccgccgcc tccccagccg ccacaaagaa
      781 cgaccgcatg tgccgcgagt cgcgcggtag gtgtgggcag ctggctggct cgcgtttggg
      841 ctgccttggc gtcgttgggt ggcggttctt cggccgtagc gttttcggaa cgcttcgggc
      901 ctaccttctt ggaaggtcag cgcgtttcag gaggtgtggt gacttcgcga tcggacgtcg
      961 acccgggcct cgagcgggac gccgagcgag gcggggtgcg cacgtgtttc cgttgggcca
     1021 cgcggcggac tgcacttgtc gtcggcgtgg caacggtgct gatgttcgcg gcgagtattc
     1081 cgttggccac gctctcgaag gccaagaacg gcagcgtcgt ggtgctgccg ttcgcggtac
     1141 tggggttcgt gcttgcacgc cggcagccgc ggaacccgat cggttggatc atgctcctgc
     1201 taggccccat cgtgatcgtc agcggcgacg ccgcgctgta ctcgttgttg gcgtatcacg
     1261 tcgacggcca tgacctgcca ctgtcgcggc ttgcggttgt gctggcgccc gattgggcct
     1321 tgctgcaggt cgccttgccg ctgccgatcc tgctctttcc ggacggccgg atcgcgtcgc
     1381 ggcggttgtg gtggacgctg tgggcatacg tggtagtcgg cgtggcctgg ctgtgtgacc
     1441 ttgccatccg tggatcggtc gcgttcacgc agcacccggt gcgcatcgac ccgatggggc
     1501 agtcggtaaa tctcgatgtc accgccggcg tctttgacga catccagaac gtgctggccg
     1561 tcgcgtatgt cgggctggtc ttgtcgtggg tgctcaggct gctggtggcc taccgcggtt
     1621 cggcaggcga ccttcgccag caactcaagt ggctgatggt gggcggggcc ctttgcgtcg
     1681 tgggaattct ttccggcgca ctgcttagtg ggatcggatc ggcactgttc agcctcatcc
     1741 tcgcgtttcc catctgcatc ggaatcgcga tcttgaagta ccggctctat gacatcgacc
     1801 gtttgatcag tcgcacgctg tcctacctca tcctcaccgc gctgctaatc ggtgtgtacg
     1861 tcggcctcgt agccctgacc actcgcgcgc tgccgctgtc gtcaccgatc ggcgtcgcgg
     1921 cgtccacgct tactgttgcg gcgttgttca atccggtgcg ccgccgcgcg cagcggctcg
     1981 tcgaccgccg cttcaaccgg gcgcgctacg acgcggacgc caccatcgcc gcattcaccg
     2041 ccgcactgcg caacgaaatc gacctcgacc gggtccagca tgatctcgtg aacgtcgtct
     2101 accgctccgt acaaccggcg cacgtctcgg tctggatccg ctccagcgca tgagccctgc
     2161 cggaaacgca cgtggttcat gtcgggtccc aacggaacgc accaaatctg ccgcgactcg
     2221 cgcggtctga cgatgacgtg cgcgtcgcgc actctccggc attggctgtc tcggtcatga
     2281 cgacgtagcg ggggccgacg acgcagcggc gacttgctca gcctccgcgc actgagcgtc
     2341 aatgctcgct gggcatgcac cgacgatttg ccggctcccg atcgggcccg gcaaggacac
     2401 cgtgcgcggt tcgaaactaa gatccgcagg gcacacgcct tgccagactt tgtcccaggt
     2461 cgtaatcgcg atcgcctgtc ggccgtagct gacgtcaaga tgatcgaagc ggcgacagcc
     2521 accctccacc gcgaggatct cgacatttga actagtcgga acagccgaca ccaacaccca
     2581 ttgccccggg gtgagcccgt tgccgccccc gccggtcacc gccgtccgaa cgccaaggaa
     2641 ggtcaaggcc gcgaccaaca cagcaccggc gacgatgggc catcgcttcc gcaagaaacc
     2701 ggacacggtc caccgcccat cagccgccga atgggtgagc gggcatgccg atcgtgcccg
     2761 attctgatcg gggctggcac agtcgtcgcc gcccgatcgg cccacctcgc gaggacaagc
     2821 cgccgctgtg caccgacgtc cacatttggc cgcattgacg cgcgttcgat cgtgccgatg
     2881 agcccgccgt ccgcctaatg cgcacccggc cgatggtttg ttttcccttc tgtgctgcgc
     2941 ctcggccttc ggctgcatac atccggtcac ccatcccgca atcatccggc taacagtctt
     3001 gcaatcatcc gcttagaagg tctgcaatca tcctgccagg agcccacgac cggtagagtg
     3061 cccgcatcga tacggcgaac gtgctgggac gcgagtaccg gcctagcgtg atagacagcg
     3121 cgttgcagcg tgccctcgcc gcggccggcg cggtggtgct cgaaggcgcc cgggccaccg
     3181 gcaagaccac gacggcgctg cacgccgcgg cgtcctgggt ctttatcgac gatgcccagg
     3241 cacaaagtct gctcgacgtc gcgccgcgtt ccctcatcga aggtgacgca cccagactgc
     3301 tcgacgagtg gcaggtggcc ccacagctat ggaacctcgt ccggcgagcg gtcgacgcca
     3361 ccgccgagcc aggcctgttc attctcaccg ggtccgctgt cccccctgac gatgtcaccc
     3421 ggcacaccgg gggcggccgg tttctccggc tgcgcgagcg gaccatgtcg tggtgggaga
     3481 aactcgactc gcctacgggc gctgtgagcc tcgctggact attcgagggg aacctgccca
     3541 agacagatct tggtgcggcc cccgatctcg acaccgtcat cgacaacctg ctacgcccgg
     3601 gcttcccggc gatgaccgcg ctggccgccg ctcagtccgc ggatcgtctg cgcggctaca
     3661 tcgacgacgt tgcacgtacc gacatccgcc gtatcgccga tatccggcat gagcctgagg
     3721 tgatcaagca acttatcgcg ggcctcgccc gatcggtcgc ctctgaggtc acctacaaga
     3781 ccctcgcggc ggatgtccgc gtcgtcgctc ccaccatcga cgaggagacc gtcagcaact
     3841 acgtcggcct acttcagcgg cttttcgtcg tggaggcgca gcggccgtgg accccgacgc
     3901 tgcgctcccg cgcgagggtg cggacatcgc cgaagttcca cctcgtcgac cctgcactcg
     3961 ctgcggcggc gctcggagcc ggagcccagc acctgcgagg agacctgaac actcttggca
     4021 ccctgtttga gagcgccgtt gtccatgatc tgaccgtgct gaccctcggc ggcgaggtca
     4081 gccactaccg ggactccaat ggcaaggaga tcgacgcgat cctcacgctt cccgacggac
     4141 gctggggtgc cgtggaggtc aagctcggtg gtcagcaggt cagcatgggc gcccggtcgc
     4201 tgcaggccgt catcgcgcag gtcgacacgg cagcgatcgg cgaaccaact ttccggcttg
     4261 tcgtcactgg aacaggcccg atcctggtca ccgacgacgg caccgtcacg gccccgctga
     4321 ctgcactcgg tccctgactg cggccgtgcc gcaatgccaa ccgaccttcg aggaaacgat
     4381 cccgatgcct aagcacaccc cagcccgcgt cacttccgac actcctatcg gtaccgatgt
     4441 cgacctcgac cgagacgaca ttcggctcgc tgatggcagc cggctgaccc aagaggtcgc
     4501 caacggcgtg gtcgagcacg ctcgccgagt ggctgggcgg ccgtcgctgt cgggcaacgc
     4561 cgaggagtcg ccgcagatcg agtttcccgt cgcgcctgag catcctagat gatcttgaag
     4621 tggtcaccga gtggtcacag aacctctgga aacgagacga tcggttttga tagtcgaagt
     4681 gactcgacgg caccgcgcat acctgtgacc tgcgcgaatg cgataatcgg cggtacgagg
     4741 ggcc
//
LOCUS       DATCBJ010000380         2025 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_473885, whole genome shotgun sequence.
ACCESSION   DATCBJ010000380 DATCBJ010000000
VERSION     DATCBJ010000380.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2025)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2025)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2025
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_473885"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..217
                     /locus_tag="VII50_07560"
     CDS             <1..217
                     /locus_tag="VII50_07560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5050743.1"
                     /translation="PRPGDIVAAPPRPGDMVSVEVTYAAPHHLVADIIHSVRRTRAGD
                     AWEARQEDGPRQPIGVQLGMPAFPSGV"
     gene            285..740
                     /locus_tag="VII50_07565"
     CDS             285..740
                     /locus_tag="VII50_07565"
                     /inference="COORDINATES: protein motif:HMM:NF016840.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEY5050744.1"
                     /translation="MLEEWLEFHRVTLLLKCEGLDDERRKARPVPTSLLSLHGLLRHM
                     AEVERNWFRRVLTRESDTPPIWYDPAIDDSELVPLDDADWDADLAAWHAECEHSRTAA
                     AARELDDTGIRRGQPCSLRWIYTHMIEEYARHNGHADLIRELVDGAVGC"
     gene            814..942
                     /locus_tag="VII50_07570"
     CDS             814..942
                     /locus_tag="VII50_07570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5050745.1"
                     /translation="MASRAIADGCRLRKPGGVVTEPGRAVDVAIVDLGTDLDVRRR"
     gene            980..>2025
                     /locus_tag="VII50_07575"
     CDS             980..>2025
                     /locus_tag="VII50_07575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5050746.1"
                     /translation="MAPAIPGANSDGGMASAWSLDGGQAILAPAGDAHPAVDRQQALC
                     TLLAAQDVSHFDVLSGNSGFSLVLAKVTIADRVLGSPEDAGVDVGTEVGVQSPPPLSP
                     FHSRLAWVGVIDPPFMSTCGADGFAPTSPSPPLVPYQLLILDAGTGADGVIYETRYYS
                     TCTGSPAFGPFVSTLTVSVSVPWRFLSRDPGGLFGTVQASVTSCDTYSGGVNTSSAHV
                     GELEFDVSRPITPCVGTHKTDQIVHAPTASDPLPLTLTHAPLGFRDAEPDAGRGTIDP
                     TALLCEKSLRDGEILAADYPTTIGDLSSQRAGPSPGHLLVNIDASKFPSSALAGWCEA
                     KSASGYAIDIVGPG"
BASE COUNT          329 a          655 c          672 g          369 t
ORIGIN      
        1 gccgcgaccg ggtgacatcg tcgcggctcc gccgcgaccg ggtgacatgg tcagcgtcga
       61 ggtgacatac gcagctccgc accatctagt cgccgacatc atccacagcg tgcgccgcac
      121 ccgcgccggc gatgcgtggg aagcgcggca ggaagatggc ccgcgccagc ccatcggcgt
      181 ccaactcggt atgcccgcat tcccctcggg agtctgaccc catcgttgac gacctgatcg
      241 atccgcgacg cagcgaacca tcgtttctgc ttagtgagcg ggcgatgctc gaggaatggc
      301 tggagtttca ccgggtcacg ctgttgttga aatgcgaagg gctcgatgac gaacgtcgca
      361 aggcgcggcc ggtaccgact tcgctgctgt cgctgcacgg gctgttgcgg cacatggctg
      421 aagtggaacg aaactggttt cgccgggtgc tgacgcgtga atccgacacg ccaccgatct
      481 ggtacgaccc ggcaatcgac gacagcgagc tcgtgccgct tgatgacgcc gactgggatg
      541 cagatctcgc agcctggcat gcggagtgtg agcacagccg gacggccgcc gccgcgcgtg
      601 agctcgacga caccggtatt cgccgcggtc agccgtgctc gctgcgctgg atctacaccc
      661 acatgatcga ggagtacgcg cggcacaatg ggcacgcgga cctgatccgt gagctcgtcg
      721 acggtgccgt cgggtgttga gttaggggaa ccgggccgtg cccgtcagcg tccgagcagt
      781 gacgaaacgg gtgccaatgc ggacaggact ggcttggcta gccgcgctat cgctgatggc
      841 tgccggttgc ggaagccggg cggtgtcgtc actgagcccg gtcgtgccgt cgacgtcgcc
      901 atcgtcgacc tcggcaccga cctcgacgtc cgccgccgct gacctctgcg tagatcccaa
      961 tccgacacga ccggccccgg tggcgccggc gatccccggt gcgaacagcg atggcggcat
     1021 ggcgtcggcg tggagtctcg atggtggcca ggcgattctc gcgccggccg gcgatgcgca
     1081 ccctgccgtc gaccgacagc aagctctgtg caccctgctc gccgctcaag acgtgagcca
     1141 cttcgatgtg ttgagcggca attccggatt cagcctggta ctcgcgaagg tgacgatcgc
     1201 cgatcgtgtg cttggttcac ccgaagacgc tggcgtggac gtcggcaccg aagtcggcgt
     1261 gcagtcgccg ccaccgttga gtccgttcca ttcgagactg gcgtgggttg gcgtgatcga
     1321 tcctcctttc atgtcgacct gtggggcgga cggattcgcg ccgacttcgc cgtcgcctcc
     1381 gctcgtcccg taccagttgt tgatcctcga tgcagggacc ggggcggacg gggttatcta
     1441 cgagacccgc tactacagta cgtgcacggg cagcccggcc tttggaccgt ttgtctcgac
     1501 attgaccgtg agcgtgtcgg tgccgtggcg gttcctgtcg cgtgacccgg gcggcctctt
     1561 tggaaccgtg caagcgtccg tgacgagctg cgatacgtac agcggcggtg tgaacacctc
     1621 cagcgcccac gttggcgagt tggagtttga cgtatcgcga ccgattacgc cctgcgttgg
     1681 cacgcacaag acagaccaga ttgtgcacgc tccgacggcc agcgatccgc ttccgctgac
     1741 gctcacacac gcgccccttg gcttccggga tgctgagcct gacgctggtc ggggcactat
     1801 cgacccgact gcgttgctat gcgagaagtc cttgagggac ggcgaaatcc tcgctgctga
     1861 ctaccccact acgatcgggg acttgtcgtc acagcgcgcc gggccgtcgc ccggacacct
     1921 cttggtcaac attgacgcca gcaagttccc gtcatcagct ctcgcgggat ggtgcgaggc
     1981 gaagtcggcg tccggatacg cgatcgacat cgtcgggccc ggatg
//
LOCUS       DATCBJ010000381         2170 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_199714, whole genome shotgun sequence.
ACCESSION   DATCBJ010000381 DATCBJ010000000
VERSION     DATCBJ010000381.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2170)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2170)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2170
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_199714"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1602)
                     /gene="rhaD"
                     /locus_tag="VII50_07580"
     CDS             complement(<1..1602)
                     /gene="rhaD"
                     /locus_tag="VII50_07580"
                     /EC_number="4.1.2.19"
                     /inference="COORDINATES: protein motif:HMM:NF006189.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional rhamnulose-1-phosphate
                     aldolase/short-chain dehydrogenase"
                     /protein_id="HEY5050747.1"
                     /translation="MAGGPTDGAAQVAALITRSNRLGADPTVTNYAGGNTSAKGDDID
                     PVTGNPVELLWVKGSGGDLGTLTEKGLAVLRLDRLRSMVGVYRGVEHEDEMVAAFDFC
                     LQGRGGAAPSIDTAMHGLVGARHVDHLHPDAGIAFATAADGEALTRQCFGDRVGWIPW
                     RRPGFQLGLEIAEAYQAHPDAIGVVLGGHGITAWGVTSDECEARSLEIIRGAQAFIDA
                     FGSPQPFGAPRAGFGALPPGERHRRAAAIAPVVRGLASTDHRQIGHFDDADEVLDFMA
                     GAKCAELAELGTSCPDHFLRTKVRPMLLDLPGDSGLEEVVARLRELHVAYRADYQAYY
                     ERHADANSPAMRGADPAIVLVPGVGMFSFGKDAQTARVAGEFYRNAIRVMRGAEAIST
                     YAPISEAEKFRIEYWRLEEAKLQRMPAPRPLASRVALVTGAGSGIGRAIAHRLSAEGA
                     CVVVADRDASSAEIVAKELGGRDKAISVRMDVTSEDEVAAAFDEAALAFGGVDLVVNN
                     AGLSISKPLAQTTLDDWDLQHDVMAR"
     gene            complement(1602..>2170)
                     /locus_tag="VII50_07585"
     CDS             complement(1602..>2170)
                     /locus_tag="VII50_07585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5050748.1"
                     /translation="HCLQLGPKAQVVVDTGHHAPGTNIEFIVALLLRAGKLGGFDFNS
                     RFYADDDLMVGAADPFQLFRIMWEVRRGGGLDASVGVAFMLDQCHNIEPKIPGQIRSV
                     MNVQEATAKAMLIDANELAAMQAAGDVLGANAVLMDAYNTDVRPLLAELREQMGLDRN
                     PMAAYAASGYAEKISAERIGGQAAGWGA"
BASE COUNT          374 a          754 c          692 g          350 t
ORIGIN      
        1 gcgggccatc acgtcgtgct gcagatccca atcgtcgagc gtggtctgcg ccagcggctt
       61 ggagatggag aggccggcgt tgttcacgac aaggtcgacg ccaccgaacg caagtgcggc
      121 ctcgtcaaag gccgcggcaa cctcgtcttc actcgtgacg tccatgcgca ccgagatcgc
      181 cttgtcgcga cccccgagct ccttcgcgac gatctcggcc gacgacgcgt cgcggtccgc
      241 aaccacgaca caggcccctt cggccgacaa tcgatgcgcg atcgcgcggc cgatgccgga
      301 gcccgctcca gtcaccaggg ccacccggct ggcgagcggt cgcggggcgg gcatccgctg
      361 gagcttggcc tcctcgagtc gccagtactc gatgcggaat ttctccgcct ccgagatcgg
      421 cgcgtacgtc gagatcgcct cggcgccacg catgacccgg attgcattgc ggtagaactc
      481 tccggcgacg cgtgcggtct gcgcgtcctt tccgaagctg aacatgccga cgccaggcac
      541 cagcacgatg gctgggtcag ctccgcgcat cgcgggcgaa ttggcgtccg cgtgccgctc
      601 gtagtaggcc tggtaatcag cccgataggc cacgtgcaat tcacgaaggc gggcgacgac
      661 ctcttcgaga cctgagtcgc ccgggaggtc gagaagcatc gggcgaacct tcgtgcgcag
      721 gaaatggtcg gggcacgacg taccgagctc ggccagctcg gcgcacttcg cgcccgccat
      781 gaaatcgagc acttcgtcgg cgtcatcgaa gtgcccgatc tgccgatgat cggtggacgc
      841 gaggcctcgc acgacgggtg cgatcgcggc cgcgcggcgg tgccgctccc ctggcggcaa
      901 cgcgccgaag cccgctctgg gcgcgccgaa cggttggggt gaaccgaacg catcgatgaa
      961 ggcctgcgcg ccgcgaatga tctccagcga tcgcgcctcg cactcatcac tcgtcacacc
     1021 ccacgcagtg atgccgtggc cgcccaagac gacaccgatc gcgtctggat gagcttgata
     1081 ggcctcggcg atctccaacc caagctggaa gccgggtcgg cgccacggga tccagccgac
     1141 ccgatcacca aagcactgac gggtcaacgc ctcgccgtcc gctgcagtgg cgaaggcgat
     1201 cccggcatcg gggtgcaggt gatcgacatg gcgcgcaccg accagcccat gcatcgcggt
     1261 atcgatcgag ggcgcggcac cgccacggcc ctgcaaacag aagtcgaacg cggcaaccat
     1321 ctcgtcttcg tgctcgactc cgcgatagac accgaccatg gaccgaagtc gatcgagccg
     1381 caacaccgca aggcccttct cggtcagggt gccgaggtca ccgccggagc ccttgaccca
     1441 caacagctcg accgggtttc cggtgaccgg atcgatgtcg tcgcctttgg ccgacgtgtt
     1501 tccgccggcg tagttcgtga cggtcgggtc agcgccgagc cggttgctcc gggtgatgag
     1561 agcagcgact tgcgcggcgc cgtcagtcgg accgcctgcc atcaggcgcc ccagccggcg
     1621 gcttggccgc cgatccgctc ggcggagatc ttctcggcat aaccggacgc cgcgtacgcc
     1681 gccatcggat tgcggtcgag tcccatttgc tcgcgaagct ccgccagcag tggccgcaca
     1741 tcggtgttgt acgcgtccat caggaccgcg ttcgcgccga gcacgtcgcc tgccgcttgc
     1801 atcgccgcca gttcgttcgc gtcgatgagc atcgctttgg ctgtcgcttc ctgcacattc
     1861 atcaccgagc ggatctgtcc ggggatcttg ggttcgatgt tatggcactg gtcgagcatg
     1921 aacgcgacgc cgaccgaagc atccagccca ccgccgcgcc gcacctccca catgatccga
     1981 aacaactgga acggatccgc tgccccgacc atcaagtcgt cgtcggcgta gaaacgtgag
     2041 ttgaagtcga acccaccgag cttgcccgcc cgaagcagca acgcgacgat gaactcgatg
     2101 ttcgtgcccg gtgcgtgatg gcccgtgtcg acgacaacct gcgccttcgg gccgagctgc
     2161 aggcaatggg
//
LOCUS       DATCBJ010000382         2071 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_187926, whole genome shotgun sequence.
ACCESSION   DATCBJ010000382 DATCBJ010000000
VERSION     DATCBJ010000382.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2071)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2071)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2071
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_187926"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..726
                     /gene="ispH"
                     /locus_tag="VII50_07590"
     CDS             <1..726
                     /gene="ispH"
                     /locus_tag="VII50_07590"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009083108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0015968 - stringent response [Evidence
                     IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HEY5050749.1"
                     /translation="VAPSVYVEASERHLRTIDATCPLVTKVHHEARRFSDEGLEILLI
                     GHANHEEVVGTAGQAPDKVKLVDGPVDAARVEVRDPSRVAWLSQTTLSVDETLETVHA
                     LRARFPQLMDPPSDDICYATQNRQAAVKEIAPASDLVIVVGSRNSSNSVRLVEVALES
                     GARASYLVDDAGEINEGWLDGVTTVGLTSGASVPDSLVLGVLSWLAERGFGAVEEIEA
                     VRETLTFALPPELRRDIKAHAQR"
     gene            complement(784..1530)
                     /locus_tag="VII50_07595"
     CDS             complement(784..1530)
                     /locus_tag="VII50_07595"
                     /inference="COORDINATES: protein motif:HMM:NF042451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6542 domain-containing protein"
                     /protein_id="HEY5050750.1"
                     /translation="MSEPAEPLPEPLFAEPVLEPLSPPRVELAAVPVRLGPAIPGRVA
                     PEPIAPELALVELIGGASAPWQQATPRPVTSQPAVTGPTALEAAEPESGAAPPAAAAG
                     ASTEARPQTGRVVSGRVVSGRGVTGRGVVVLILILTGLVGVLEVAYAGHRGAAFAVAF
                     MLTSAIGALVVRRRDLPTALIAPPLLYCVLIAVLSAIDTHDQSGGFITRKAVYIGNAF
                     VTGAPTLWAGTAAAIAIALYRHRTGRQLRS"
     gene            complement(1577..>2071)
                     /locus_tag="VII50_07600"
     CDS             complement(1577..>2071)
                     /locus_tag="VII50_07600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HEY5050751.1"
                     /translation="FIPAETFLNAALEQDPALLQYAFEKNVVIATPATLIALLRTVGY
                     TWRQEALAANAHDVLTLGRELYARLSTMGRHVGTLGIRLNSAVQSYNDTVASLESRVF
                     TTARRLVDLKVTTEELEAPKPIELVARKVQKSELVASANETLVSLRPPMPDPLFEPGE
                     HAAG"
BASE COUNT          334 a          658 c          752 g          327 t
ORIGIN      
        1 gtcgcaccgt cggtttacgt cgaagcgagc gagcgtcatc tgcggacgat tgatgcgacc
       61 tgccccctgg tcaccaaggt gcatcacgag gcacgccggt tctctgacga gggtcttgag
      121 atcctgctca tcggtcacgc caatcacgaa gaggtcgtcg gtaccgcagg ccaggcgccc
      181 gacaaggtca agcttgtcga cggcccggtt gacgccgcgc gtgtggaggt gcgcgatccg
      241 tcgcgggttg cgtggttgtc gcagaccacg ttgtcggtcg acgagacact cgagacggtc
      301 cacgcgcttc gtgcgcggtt cccgcagctg atggatccgc cgagcgacga catctgctac
      361 gccacgcaga accggcaggc cgcggtcaag gagatcgcgc ccgcgtccga tctggtcatt
      421 gtcgtcgggt cacgcaactc gtcgaactcc gtgcgactgg tcgaggtggc gctcgaaagt
      481 ggtgcgcgtg cctcgtatct ggtcgacgat gctggcgaga tcaacgaagg ttggctcgac
      541 ggtgtgacca cggtcggtct cacgagcggt gcgtcggtgc ccgactcgct ggtgctcggc
      601 gtgctgtcgt ggctggccga gcgcggcttc ggcgccgttg aggagatcga agcggtacgc
      661 gaaacactca cctttgcgct gccgccggaa ctgcggcgcg acatcaaggc gcacgcacag
      721 cgctagaagc gtttgctgga cggcgcggtg cgtgtcgacg gcgtggtgcg tgccggaccg
      781 cgatcagctg cgcagttggc gaccggttcg gtggcggtac aaggcgatcg ctatggcagc
      841 ggcggtgccg gcccacaacg tcggcgcgcc ggtcacgaac gcgttgccga tgtagaccgc
      901 cttgcgggtg atgaatccgc cgctctggtc gtgggtgtcg atagcgctca gcaccgcgat
      961 gaggacgcaa tacagcaacg gcggcgcgat gagcgccgtc ggcaggtcac ggcggcgcac
     1021 gacgagggct ccgatcgcgc tggtgagcat gaacgcgacc gcgaatgccg caccgcggtg
     1081 gccggcgtaa gcgacctcga gcacgccgac gagcccggtg aggatcaaga tcaacacgac
     1141 gacgccgcga ccggtgacgc ctctgcctga gacaacgcgg cctgagacga cgcggccggt
     1201 ctgcgggcgg gcctccgtcg aagcgcccgc agcggccgca gggggggccg ccccggattc
     1261 gggttcggcg gcttcgagcg cggtgggtcc ggttacggcg ggctgcgacg tgacgggtcg
     1321 cggcgtggcc tgctgccatg gggccgaggc acccccgatc agctccacga gtgccaactc
     1381 cggcgcgatc ggctccggcg ccacccttcc gggaatcgct gggccgagcc tcaccgggac
     1441 ggcggctagc tcgacacgcg gcggcgaaag cggctcaagc accggctcag cgaacagcgg
     1501 ctcgggcagc ggctctgcgg gttcggacac ggacctacgc taagccgcag agcgccgata
     1561 acgcatcacg actcgctcag ccggctgcgt gttcgcctgg ttcaaacagc ggatcgggca
     1621 tcggcgggcg aagcgacaca agcgtctcgt tcgcggaggc gaccagttcc gacttctgaa
     1681 ccttgcgtgc aacgagctcg atcggcttgg gcgcctcgag ctcctcggtg gtcaccttca
     1741 ggtcaaccag ccgccgggcc gtggtgaaca cgcgggactc aagcgaggcg acggtgtcgt
     1801 tgtagctctg cactgcggag ttgaggcgga tgccgagcgt gccgacgtgg cgccccatgg
     1861 tcgagagcct ggcgtacagc tcgcggccca gcgtgaggac gtcgtgggca ttggcggcaa
     1921 gggcctcctg gcgccacgtg taaccgacgg tgcgcagcag cgcgataagg gtggccggcg
     1981 ttgcgatgac cacgttcttc tcgaacgcgt actgcaacag cgctggatcc tgctcgaggg
     2041 ccgcgttgag gaatgtctcc gccgggatga a
//
LOCUS       DATCBJ010000383         2139 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_353409, whole genome shotgun sequence.
ACCESSION   DATCBJ010000383 DATCBJ010000000
VERSION     DATCBJ010000383.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2139)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2139)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2139
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_353409"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..568
                     /locus_tag="VII50_07605"
     CDS             <1..568
                     /locus_tag="VII50_07605"
                     /inference="COORDINATES: protein motif:HMM:NF013073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HEY5050752.1"
                     /translation="AAALAAQKRAAAMALQQAAASHQGNGANGSGDGGSNSGGSGGGI
                     VPPGGQSISTPAQRQAAIAFAESQIGVWYRWAGAGEVGPTVTSTGVQNLPGYDCSGLT
                     MRAYQAVGISLAHYTGAQWDDGLHVSQAQLQPGDLVFFATNTNDPSTIHHVGIYIGNG
                     QMVDAPETGEQIGIHNAFRPDYIGAVQL"
     gene            complement(592..1080)
                     /locus_tag="VII50_07610"
     CDS             complement(592..1080)
                     /locus_tag="VII50_07610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019510803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HEY5050753.1"
                     /translation="MEFDVTIEIPKGHRNKYEFDHETGRIRLDRMLFTATRYPSDYGF
                     IDDTLGNDGDPLDALVLVDEPTFPGCLIKCRAIGMFRMTDEKGGDDKVLCVPATDPRH
                     SHLRDINDVPPFDRLEIQHFFEVYKDLEPGKSVEGATWTDRAEAEVEILASRARWKNL
                     QH"
     gene            1209..>2139
                     /locus_tag="VII50_07615"
     CDS             1209..>2139
                     /locus_tag="VII50_07615"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:NF014200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004185 - serine-type carboxypeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HEY5050754.1"
                     /translation="MTVRGGHRVLRIVLVIVLMVVAVAVGGVALYASTSDESSGTSAA
                     GAAATPTRSPTATPSGITRSSAPASRPPAASAPPAAPALWQHIAAPAAPLLLPVTLDT
                     ANDATTDDPTTVGLTSVLTPLLADHAFAGDDVAMLVADATTGQLLFDHDGAEVVPPAS
                     TAKLAVAVAALQVLGPNARLTTRVLQGSSPSRIVLVGGGDPTLSGPAAVGLFSPGYPT
                     PASLTTLALQTAAHLRARGTISVSLGYDASLYVGATLAPGWKPIYQTEGDVAPVSALE
                     VDEGHPNLAKPATATDPAAAAAGEFAALLTSDGL"
BASE COUNT          354 a          717 c          711 g          357 t
ORIGIN      
        1 ggcagccgca ctcgccgcgc agaagcgcgc ggcggcgatg gccctgcaac aagcggccgc
       61 atcccaccag ggcaacggcg ctaacggcag cggcgacggg ggaagcaata gcggcggcag
      121 cggtgggggc atcgtgcccc caggcggtca atcgatttcg accccggccc agcggcaggc
      181 agccatcgcg ttcgccgagt cgcagatagg cgtctggtac cgctgggctg gggccggcga
      241 agttgggccg acagtgacct cgaccggcgt tcagaacctg cccggttacg actgttcagg
      301 gctcaccatg cgggcctatc aagcggtcgg catctcactc gcccactaca ccggcgcgca
      361 gtgggacgat ggtctgcacg tgtcgcaagc tcagttgcag cccggtgacc tcgtcttctt
      421 cgcgaccaac accaatgacc cgagcacgat tcatcacgtg ggcatctaca tcggcaacgg
      481 ccagatggtc gacgctccgg agaccggcga gcagatcggt atccacaacg cgtttcgccc
      541 cgactacatc ggcgcggtcc agctctaagg cgcggtccag ttctaagtag gtcagtgctg
      601 aaggttcttc caacgcgccc gcgacgccaa gatctcgacc tcggcctccg cccggtcggt
      661 ccatgtcgca ccctcgaccg actttcctgg ttcgaggtcc ttgtagacct cgaagaagtg
      721 ctggatttcc aaccggtcga agggcgggac atcgttgatg tcgcgcaggt gactatggcg
      781 cgggtccgtc gcaggcacgc agaggacctt gtcgtcgccg cccttttcgt cagtcatccg
      841 gaacatgccg atcgcacgac acttgatgag gcaaccggga aaggttggtt cgtcgaccag
      901 aacgagtgcg tcaagcggat caccgtcgtt gccgagcgtg tcgtcgatga atccatagtc
      961 ggacggatag cgagtcgcgg tgaacagcat ccggtcgagc cggatccgcc cggtctcgtg
     1021 atcgaactcg tacttgttgc gatgaccctt ggggatctcg atcgtgacgt cgaactccat
     1081 cgcgctccca gtttctggcg agcatcgccc cagatgaggg cgaacatcgc tgccggcaac
     1141 ccgtcgttcg ttagtctcgc gtaccgcggt atgacgagtg aggtaggggc acgcaagcta
     1201 ggggggcgat gacggtgcga ggcggccacc gcgtgctgcg catcgtcctg gtcatcgtgt
     1261 tgatggtggt ggcggtcgcg gtcggcggtg tcgcgctcta cgcatcgacg tccgacgaat
     1321 caagcgggac gtcggcggcg ggtgccgctg ccacgccgac ccgttcgccg accgcgacgc
     1381 cgagtggaat cacacggagc tcggcgccgg cgtcgcgtcc acctgcggcg tccgctccac
     1441 ctgccgcgcc tgcgctttgg cagcacatag ctgcgcctgc ggctccgctg ttgttgcccg
     1501 tcacgctcga caccgccaat gacgccacca ctgacgatcc gacgacggtc gggctcacga
     1561 gcgtgctgac cccgctgctc gcggatcatg cattcgccgg ggacgacgtc gccatgctgg
     1621 tggccgacgc cacgaccggc cagctgctgt tcgaccacga tggggcagaa gtcgtgccgc
     1681 cggcctcgac cgcgaagctc gcggttgccg tggcggcgtt gcaggtcttg ggaccgaatg
     1741 ctcgcctgac aactcgggtg ctgcagggca gtagcccgag ccggatcgtc ctcgtcggcg
     1801 gtggggatcc gacattgagc gggccggccg ccgtcggact cttcagcccg ggctacccga
     1861 caccggcgag cctgaccacc ctggcgcttc agacagcggc gcacctgcgc gcgcgcggga
     1921 ccatttccgt cagcctcggc tatgacgcct ctttgtacgt cggggcgacg ctcgcgcccg
     1981 ggtggaaacc catctaccaa accgagggcg acgtcgcacc cgtatcggcg ctggaagtcg
     2041 acgagggaca tccgaacctg gccaaacccg cgacggccac cgacccggcg gctgccgcgg
     2101 cgggcgagtt cgccgcgttg ctgaccagcg atgggctga
//
LOCUS       DATCBJ010000384         4675 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_260736, whole genome shotgun sequence.
ACCESSION   DATCBJ010000384 DATCBJ010000000
VERSION     DATCBJ010000384.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 4675)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4675)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4675
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_260736"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            171..1253
                     /locus_tag="VII50_07620"
     CDS             171..1253
                     /locus_tag="VII50_07620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5050755.1"
                     /translation="MHRAQSPAYEPSGLVLTRRDLVTLLGSAAVLVACGGKSHTTSPI
                     TVTSGSIGNSAPPQTGGGKSASGNLVQIGGPHATGAEAMSVADAQREAQVANGHWQGG
                     WHDATGASGDSDLVIAIDGASRTAKATVSFGDKLLGTAMPSITYDIDLLSFTMTADSY
                     NVASPQFGQVNIVPGGSTNASGTARAIPGQPTIDHVDVDGTMAGRRVDVAYTVHYTDG
                     HSVKGTMAWTSTGGRATPAAPGSAGAPTTADISSGSYGAALLDEKSLTTIVGEPFGAP
                     TSNGGNLLYDVGIATSNAEANATSGDYSVEYTGYVGTNAADTDAFWKKQNPGLPTTPG
                     PWLSGFYYPAAQGFYAELPTRVLLVR"
     gene            complement(1377..1772)
                     /locus_tag="VII50_07625"
     CDS             complement(1377..1772)
                     /locus_tag="VII50_07625"
                     /inference="COORDINATES: protein motif:HMM:NF024092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEY5050756.1"
                     /translation="MPTLADGDVVRHLYQAIADRDFVAAERCFADDVVWYLPGDSPIA
                     GDHHGWSQIRDDFMAKLGPLSGGTFRADLVDVAIGERCIVAVQHATASYRGKVLDITA
                     CQLITVRDGLIHEVRGHYSDQDALDAFWQ"
     gene            1837..2091
                     /locus_tag="VII50_07630"
     CDS             1837..2091
                     /locus_tag="VII50_07630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009075408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HEY5050757.1"
                     /translation="MKLKLDLHEIYNRGQDIDSALRAVIDEAVTKKAPLVEIIPGKGS
                     GALKKHVLRFLDQKEIKALYHRVEKDSDNFGRVFVHFRWK"
     gene            2143..2460
                     /locus_tag="VII50_07635"
     CDS             2143..2460
                     /locus_tag="VII50_07635"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEY5050758.1"
                     /translation="MHPFEAVADPTRRRILELLGDGEQSAGDLASQFDISRPAVSRHL
                     RMLRETGLVEARGDAQRQLYSLRREGVDEIGEWVDTIRAFWSQRLDALDSHTRPTRRR
                     DAR"
     gene            2457..2945
                     /locus_tag="VII50_07640"
     CDS             2457..2945
                     /locus_tag="VII50_07640"
                     /inference="COORDINATES: protein motif:HMM:NF019928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEY5050759.1"
                     /translation="MTGSVFGDVRSEGHGRTVRFERHFQAPINEIWAACTEPEELSHW
                     FAEVSGDLRVDGAAFIDFDNGEHTPVVIRQCEPPSLLVADWAFGDDAMSTLRVDLSSS
                     SGGGTTMVLEHRMLPTSTGVGYAAGWHAYLDRLAEQLSGNEITSWSRRFDELIGDYQA
                     PA"
     gene            complement(2942..3358)
                     /locus_tag="VII50_07645"
     CDS             complement(2942..3358)
                     /locus_tag="VII50_07645"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(deoxy)nucleoside triphosphate
                     pyrophosphohydrolase"
                     /protein_id="HEY5050760.1"
                     /translation="MADCAAVNAYPQVVVGAAIVNGERVLAAQRSEPPSLAGFWEFPG
                     GKVDPGETDEAALIRECREELAIDIMLGARIGRDWPIGEHGVLRVWLAGVVAGEPTAI
                     EHAALRWLTVDELHDVEWVPADLPIVDRLAAMMRGR"
     gene            3368..3691
                     /locus_tag="VII50_07650"
     CDS             3368..3691
                     /locus_tag="VII50_07650"
                     /EC_number="4.2.1.96"
                     /inference="COORDINATES: protein motif:HMM:NF002017.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008124 -
                     4-alpha-hydroxytetrahydrobiopterin dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006729 - tetrahydrobiopterin biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4a-hydroxytetrahydrobiopterin dehydratase"
                     /protein_id="HEY5050761.1"
                     /translation="MTPRRSTDDGGFMQHQLSAVDVARALAELTDWTGDTTSLRRSIK
                     ASSFAAGIQLVDAVAEIADAMDHHPDIDIRWTTITFTCSTHSAGGVTELDVALARRIN
                     DLAGS"
     gene            3795..>4675
                     /locus_tag="VII50_07655"
     CDS             3795..>4675
                     /locus_tag="VII50_07655"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY5050762.1"
                     /translation="MPPVIRVENLVKEFRRPRKQEGRFGALRTLFTRQFDTVRAVDQV
                     NFAIEPGELVGYIGPNGAGKSTTIKMMTGILVPTSGTVDVGGLVPWRERERNAMQIGV
                     VFGQRSQLWWDLPLIESFNLIAKLYRVPEAKYRRNLERFIALLDMEEFLDRPVRQLSL
                     GQRMRGDLAASMLYEPQILYLDEPTIGLDIVAKENMRIFIEEMNRDSGTTIVLTTHDL
                     ADVERLCRRLILIDHGHVLYDGGVEQLKTKYAPYRILVVELEADAVSNGRLIDAADVP
                     GAEIVEQADGVARIQFES"
BASE COUNT          898 a         1588 c         1441 g          748 t
ORIGIN      
        1 gaccgccgcg cccgccatga cacctcccct cgccgcccgg ccactgtaac cggaccggtc
       61 acgatagccg gaccgagcac cgggaccgca acgagattgc cttgcatacg ctctgacgat
      121 ccgggtcggt cctgacgccg ccgaccccag ccttcgggag tccgagcgcc atgcaccggg
      181 cacagtcacc cgcatacgaa ccgagtggcc tcgtcctcac ccgacgcgac ctggtcaccc
      241 tacttggatc tgctgcagtg ctcgtcgcat gcggcggcaa gtcgcacacg acgtctccga
      301 tcaccgtcac ttccggcagc atcggcaact cggcgccgcc tcagaccggc ggcggcaaat
      361 cggcgtccgg caatctcgtt cagatcggcg ggccgcacgc gacgggtgcc gaggcgatgt
      421 cggtggccga cgcgcagcgc gaggcgcagg tcgcgaacgg acactggcag ggcggctggc
      481 acgacgcgac aggcgcgagt ggcgacagtg acctcgtgat cgcgatcgac ggtgcgagcc
      541 gcactgccaa agccaccgtg tcgttcggcg acaagctcct cggaaccgcg atgccgtcca
      601 tcacctacga catcgacctg ctgtcgttca cgatgactgc cgacagctac aacgtggcga
      661 gccctcagtt cggccaggtc aacatcgttc ccggcggcag caccaatgcg tccggcaccg
      721 cccgcgcgat tcctgggcag cccacgatcg atcacgtcga cgtcgacggc accatggcgg
      781 gtcgacgcgt cgacgtcgct tacaccgtgc attacaccga cggtcacagc gtcaaaggca
      841 caatggcctg gacgtcgacg ggcggacgtg cgaccccggc cgcaccgggc agcgccgggg
      901 caccgaccac ggctgacatc tccagtggct cgtatggggc tgcactgctc gacgaaaaat
      961 cgctcaccac gatcgtcggt gaaccgttcg gcgcacctac gtccaacgga ggcaacctcc
     1021 tctacgatgt tgggattgcc acgagcaacg cagaagcgaa tgcgacctcg ggcgactaca
     1081 gcgtcgagta caccgggtac gtgggcacca acgcggccga taccgacgca ttctggaaga
     1141 agcagaaccc aggattgccg accactcctg gaccctggct atccggcttc tactaccccg
     1201 cggcccaggg gttctacgcg gagttgccga cgcgggtcct actggtcagg tgattccgct
     1261 gaaggaaacg gcgccaccga ccacgcagca gtccacgatg atccaacagt gggctgcgca
     1321 gatcgccacc gcactcgcga acagcctcaa atccggttga ccgcagtacc gccccgctac
     1381 tgccagaagg cgtcgagcgc atcctgatcc gagtagtgcc cgcgaacctc gtggattaga
     1441 ccgtcgcgaa cggtgatgag ttggcaggca gtgatatcga gcaccttgcc tcggtaggac
     1501 gccgtcgcgt gttgcaccgc gacgatgcac cgctcgccga tcgcgacatc aaccaggtcc
     1561 gcgcgaaacg tgccaccgga caatgggcct agcttggcca tgaagtcgtc gcggatctgc
     1621 gaccatccgt ggtggtcacc cgcgatgggg ctgtcgcccg ggagatacca cacaacgtcg
     1681 tccgcgaaac agcgttctgc ggcgacgaaa tcgcggtcgg cgatggcttg atacaagtga
     1741 cggaccacat cgccgtcggc aagagtaggc atcgtcgtat ggtgccatgc gagccctgcc
     1801 cgcgccgtcg tggaccgccg ggcggacgat ggagccgtga agctcaagct ggatctgcac
     1861 gagatctaca accgaggcca agacatcgac agcgcgctgc gcgccgtaat cgacgaagcc
     1921 gtgaccaaga aggccccgct cgtcgaaatc atccctggca aggggtcggg agcgctgaag
     1981 aaacacgtcc tgcgattcct tgaccagaag gagatcaagg cgctttacca ccgcgtggag
     2041 aaggactccg acaacttcgg ccgggtgttc gttcacttcc gctggaaata gagccgcttg
     2101 cgtagcttga cagttacgta actctggggt tacatacgaa tcgtgcaccc attcgaggcg
     2161 gtcgccgatc ccactcgtcg tcgcattctc gagctgctcg gcgacggcga gcaaagcgct
     2221 ggcgacctcg catcgcagtt cgacatctcg aggccggccg tgtcgcgaca ccttcggatg
     2281 ctgcgcgaga ccggtcttgt ggaggcccgc ggcgatgccc agcgtcagct gtactcgctg
     2341 cgtcgtgaag gcgtggatga aatcggcgag tgggtcgaca cgattcgagc attctggtcg
     2401 caacgcctcg atgccctcga cagccatacc cgcccgacca gaagaaggga cgcccgatga
     2461 cgggatctgt attcggagac gtccgatccg aaggacacgg tcgcacggtc cggttcgaga
     2521 gacatttcca ggcgccgatc aacgagatct gggccgcatg caccgagccg gaggaactct
     2581 cgcattggtt cgcggaggtc tccggcgacc tccgcgtcga cggcgcggcg tttatcgact
     2641 tcgacaatgg cgagcacacg cctgtggtga tccgccaatg cgagccaccg agcttgctcg
     2701 tcgcggactg ggcgttcggg gacgacgcga tgtcgacctt gcgcgtcgat ctcagctcgt
     2761 ccagcggcgg tgggacgacc atggtccttg aacaccgcat gctgccgact tcgaccggcg
     2821 tcggctacgc ggcgggctgg cacgcctacc tcgaccggct agccgagcag ttgtccggca
     2881 acgagatcac cagctggagc cggcgcttcg acgagttgat cggtgactac caggcacccg
     2941 cctagcgacc ccgcatcatc gcggcgagcc gatcgacgat cggtagatcc gcaggcaccc
     3001 attcgacgtc gtgcagttcg tcgacggtga gccaccgcaa cgccgcgtgc tcgatcgccg
     3061 ttggctcgcc cgcaacaaca cccgccagcc atacccgcag gacaccgtgc tcaccgatcg
     3121 gccaatcgcg accgatccgc gcgccgagca tgatgtcgat ggcgagttct tcgcggcact
     3181 cacggatgag cgcagcctca tcggtctcgc ccgggtcaac ttttccccca ggaaactccc
     3241 agaaaccggc gagcgacggc ggctcggacc gctgggcggc gagcacccgc tcgccgttca
     3301 cgatggcagc accgacgacc acctgcgggt aggcgttcac agcggcgcag tctgccacga
     3361 tcccaccttg actccacgac ggtcgacaga cgacggtgga ttcatgcaac accaactgag
     3421 cgccgtcgac gtggctcggg cgttggccga gctcacggac tggaccggcg acaccaccag
     3481 tctccggcgc agcatcaagg catcgagttt cgccgccggc atccagctcg tcgatgcagt
     3541 tgcggagatt gccgatgcca tggaccacca ccccgacatc gacatcagat ggacgacgat
     3601 caccttcacc tgctcgacgc actccgcggg tggggtcacc gagctcgatg tcgcgctcgc
     3661 tcgccgaatc aacgatctcg ccggcagctg accgcggtaa ctcagtcgcc ttccgccgcc
     3721 ggattgcggt agaccagtac ccgatcatct gaggtctagt cctttgcatg cccggaacac
     3781 atcaggagac gtccgtgccg ccggtgatcc gcgtcgagaa cctcgtgaag gagttccggc
     3841 gcccgcgcaa gcaggaaggt cgcttcggcg cccttcgaac gctgttcacc cggcagttcg
     3901 acacggtgcg cgcggtcgat caggtgaatt tcgcgatcga gcccggcgag ctcgtgggct
     3961 acatcgggcc gaacggcgca ggcaagagca ccaccatcaa gatgatgacc ggcatcttgg
     4021 tgccgaccag cggcaccgtg gacgtcggcg gcctggtgcc atggcgcgaa cgcgaacgca
     4081 acgccatgca gatcggcgtt gtgttcggcc agcgcagcca gttgtggtgg gacctcccgc
     4141 tcatcgaatc gttcaacctc atcgcgaagc tgtatcgggt gcccgaggcg aagtaccgac
     4201 gcaacctcga gcgcttcatc gcgctgctcg atatggaaga gttcctcgac cgacctgtac
     4261 gccagttgag cctcggccag cgaatgcgcg gcgacctcgc ggcgtccatg ctctacgaac
     4321 cacagattct ctacctcgac gaaccgacga tcggcctcga catcgtcgcc aaagagaaca
     4381 tgcgcatctt catcgaggag atgaaccgcg attccgggac gacgatcgtg ctcaccaccc
     4441 acgacctcgc cgatgtcgag cgcctctgcc gccggctgat cctgatcgac catggccatg
     4501 tgctctacga cggcggcgtt gaacagctga agaccaagta cgcgccgtac cgcatcctgg
     4561 tcgtcgagct tgaggcggac gccgtgagca acggtcgtct catcgacgcc gccgacgttc
     4621 ccggtgccga aatcgtcgaa caggcggacg gcgtggcgcg catccaattc gaatc
//
LOCUS       DATCBJ010000385         5293 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_129204, whole genome shotgun sequence.
ACCESSION   DATCBJ010000385 DATCBJ010000000
VERSION     DATCBJ010000385.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 5293)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5293)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5293
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_129204"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..799)
                     /locus_tag="VII50_07660"
     CDS             complement(<1..799)
                     /locus_tag="VII50_07660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5050763.1"
                     /translation="MALTLGLAWVTDVVNSHNNDRLLAQQVQQTAAAISSALPVVQSQ
                     LVDAVQVAIDTNGNAQTFQRFVAAGTVSTGNSGSISLWRLTNGHAQEITAVGPALSLV
                     NAHLDSAFFATVHPRTSLFVTGILPGTSPRLGYAEMPVGDTSGLMVYRETSLPANRRV
                     AVPPSSPFGRLKFALYLGSKPLAADLLEATGSTPIRGHTSSTSLPFGDTFITMVGASG
                     SQLAGGLSAALEWIVLGVGFALALGSGATVEYALRRRLFAEGLARDNG"
     gene            951..1553
                     /locus_tag="VII50_07665"
     CDS             951..1553
                     /locus_tag="VII50_07665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5050764.1"
                     /translation="MSCNHEDQEPGAAVCRHASRDLPPEPRSSSVARAFLRSCFTDWD
                     LMELYDDAALGLSELVANAVMHAATPLVVAVSCEGGMVEIAVHDGNPSMPAIRPQRTD
                     IDGDIQRAIAAEAATAEPFDERDPRINVGAAGSLAGGRGLLLVDAVSAQWGVSPLSDG
                     KAVWIRTPVPQGWPHGADCPCADAEQSTVLGSGRLVVHRG"
     gene            1691..1960
                     /locus_tag="VII50_07670"
     CDS             1691..1960
                     /locus_tag="VII50_07670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5050765.1"
                     /translation="MTTSAVPAPTTTSLSQVFDDWQRAGAALRTAESRGACDDEVLPL
                     AEAVLRTRVAVLRDRIAAGWQPSDEMMRDLEVDTQLMLEADARGT"
     gene            1957..2922
                     /gene="ligD"
                     /locus_tag="VII50_07675"
     CDS             1957..2922
                     /gene="ligD"
                     /locus_tag="VII50_07675"
                     /inference="COORDINATES: protein motif:HMM:TIGR02779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003910 - DNA ligase (ATP) activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-homologous end-joining DNA ligase"
                     /protein_id="HEY5050766.1"
                     /translation="MSPLPLVRPMLAVAGPIPVRDDGRFAVEMKWDGVRAVSYWDGGQ
                     LRLLSRNDNDVTPGYPELAALGSLLGSHGAILDGEIVAYDRGRPSFEALQARLHVRGA
                     KVNQLAAEVPVTLLLFDIMQLDGTATTGLTYTQRRELLEGLDLTGDHVLTPPAFHDEP
                     KTVLAASLAQGLEGVVAKAVDSTYLPGRRSPSWIKVKHEHTQEVIIGGWKPGEGGRAG
                     RIGSLLLGINNDAGELIYVGHVGTGFTDRVLADLASKLEPLHRKTSPFTDEVPRLHAR
                     DAVWVTPSLVGEVSYGEFTREHRLRHPSWRGLRPDKSPTDVHREP"
     gene            2953..3432
                     /locus_tag="VII50_07680"
     CDS             2953..3432
                     /locus_tag="VII50_07680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009077677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3291 domain-containing protein"
                     /protein_id="HEY5050767.1"
                     /translation="MTFELAQVNIGRLVAPLDHAQLADFVARLEPVNAVADNAPGFVW
                     RLQTDDGDATSIRAFEWDAGDSAGVIVNMSVWTGVDELAAFVFGEMHRELLRRRREWF
                     HRMSEAYTVCWWVPAGHRPTTDEAEDRIRNLRANGPSPAAFTLKDRYPRPDEHVSHA"
     gene            complement(3448..4695)
                     /locus_tag="VII50_07685"
     CDS             complement(3448..4695)
                     /locus_tag="VII50_07685"
                     /inference="COORDINATES: protein motif:HMM:NF037982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HEY5050768.1"
                     /translation="MGPGLVTGASDDDPSGVATYSQAGAQFGLGLLWVALLTLPLMAA
                     VQEICDRTALATGRGLGELATEKFKARTRPVLIVLISALIVANALNISADLVAIGSGM
                     NLLHAGPTWVWALVAGVVITAMVIMGSFTEIARVFKIICLALLTYFVVLFSIHVNWGQ
                     VGLRTVVPHLTLSKSYFALLVAVLGTTISPYLFFWQSAHRLEELRAEPAGGDDAVPLT
                     HRSTADAKYKSRTARGDVFTGMAFSNAVMFAIIVVTATTLHGKAISSAADAASALKPV
                     AGHWSSVLFALGFIGTGMLAIPVLAGSGAAGMAGLLGKRWGFSRSVRKAPVFYGLVAV
                     GTIGGTILSLLSTNPIHLLVLVAILNGIAAAPFLFVVMVVARDRAIMGEYRNGKISNV
                     LGWLAFALMAAAALVMLAQYANL"
     gene            5013..5168
                     /locus_tag="VII50_07690"
     CDS             5013..5168
                     /locus_tag="VII50_07690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019514767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6131 family protein"
                     /protein_id="HEY5050769.1"
                     /translation="MIVLGIVLLIVGIVLNVGILYTLGIVLVVVGAVLFLLGSAGHAV
                     GGRSHYW"
BASE COUNT          932 a         1720 c         1770 g          871 t
ORIGIN      
        1 ggccattgtc tcgggcgaga ccctcggcga acaatcgtcg gcgcagcgcg tactcgacgg
       61 tcgcgccgct gcccaacgcg agggcgaatc cgacgccgag gacgatccac tccagggctg
      121 cggacaagcc gccagcaagc tggctccctg acgccccgac catcgtgatg aacgtgtcgc
      181 cgaacggcag ggacgtcgac gacgtgtggc cgcggatcgg cgtgctgccg gtggcttcga
      241 gcaggtcagc agcaagcggc ttggacccga ggtagagcgc gaacttcaag cgcccaaacg
      301 gcgaggacgg cggcactgcc acccgtcggt tggctggcag ggaggtctcg cggtacacca
      361 tcagtccgct cgtatcgccc accggcattt cggcgtaccc gagccgcggc gatgtgccgg
      421 gcaggatccc ggtgacgaac agagatgtgc gcgggtgcac ggtcgcgaag aaggcactgt
      481 cgaggtgtgc gttgaccagt gacaaggcag gtccgacggc ggtgatttct tgtgcgtgcc
      541 cgttggtcag gcgccagagc gagatcgacc cggagttgcc tgtcgacacc gtcccggcgg
      601 ccacgaaacg ttggaaggtc tgggcattgc cgttcgtatc gatcgcgacc tgaacagcat
      661 cgacgagctg cgactgcacg accggcaatg cactggagat cgccgccgcg gtctgctgga
      721 cctgctgggc aagcaagcgg tcgttgttgt gggagttgac cacatcggtg acccatgcca
      781 agcccagggt cagtgccacg ccgatcccga ccacccctgc actgagcggg cgtagccgga
      841 gcgcgaacat ttgcgtgagt ctggcacatc gcccctaccc ggaacaggct tggcacagcc
      901 gcgcggggcc ctccgccaga gggttttgcg gggcgtcggc gtgggcaggg atgtcctgta
      961 atcatgagga tcaggaaccg ggggccgcag tgtgccggca cgcgtcgcgc gacctgcctc
     1021 ctgagccgcg ctcgtcctcc gtcgcccgcg cgttcctgcg cagctgcttt acagactggg
     1081 acctcatgga gttgtacgac gatgcggcgc tcgggctgag cgaactcgtc gccaacgcgg
     1141 tcatgcacgc cgctacgccc ctcgtcgtcg cggttagctg cgagggaggc atggtggaaa
     1201 tcgcggtaca cgatgggaat ccatcgatgc cggcgattcg gccgcagcgg accgacatcg
     1261 acggtgacat ccaacgggcc atcgccgcgg aagccgcaac tgccgagccg ttcgacgaac
     1321 gtgatccccg gattaacgtc ggcgccgctg ggtcgctggc aggcggacgc gggttgctgc
     1381 tcgtcgacgc cgtctccgcc cagtgggggg tgagcccgct cagcgacggg aaggccgtct
     1441 ggattcgcac tccggtgccg cagggatggc cgcacggggc cgattgccca tgcgcggacg
     1501 ccgagcaatc gaccgtcctc gggtcgggtc ggctcgtcgt gcaccgcggc tagcgaaccc
     1561 cgaacgagcg cccgggcgcg acacccgccg ttgtgtcgcc actgtgccgc tcgtagagtt
     1621 tgcgtcgttt gggccagtac gcacgcgcct ccgggcccct gggtggcgag cttctggagg
     1681 gttgtgtccg atgaccacgt ctgccgtacc tgcgcccacg accacatccc tctcgcaagt
     1741 gttcgatgac tggcagcgcg ccggtgcagc gctgcggacc gccgaatcgc gcggggcctg
     1801 cgacgacgag gtgcttccac ttgccgaggc ggtgcttcgg acgcgggtcg cggtgttgcg
     1861 cgaccggatc gctgccggct ggcagccgtc cgacgagatg atgcgcgacc tcgaggtcga
     1921 tactcaactc atgctcgagg ctgacgcacg cggaacgtga gcccgcttcc actcgtccgc
     1981 ccgatgctcg ccgtcgccgg accgattccg gtgcgcgacg acggccggtt cgccgtcgag
     2041 atgaagtggg acggcgtgcg cgccgtttcc tattgggacg gcgggcagtt gcggctgcta
     2101 tcgcgcaacg acaacgacgt cacgccgggc tatcccgaac tggcggcgct cggatcgctc
     2161 ctcggttctc acggcgcgat tctggacggc gagatcgtcg cctacgaccg cggacggcca
     2221 tcgttcgagg cattgcaggc acgcctgcac gtgcgcggcg cgaaggtgaa ccagctggct
     2281 gccgaggtgc cggtgaccct cctgttgttc gacatcatgc agctcgatgg cactgcaacg
     2341 accgggctga cctacacgca acgacgtgag cttctcgagg gactcgacct caccggcgac
     2401 catgtgctga ccccgccagc ttttcacgat gagccgaaga cggtgctcgc agcgagcctc
     2461 gcgcaaggac ttgagggtgt ggtcgcgaag gccgtcgaca gcacctacct gcccggccgg
     2521 cgatcaccgt cgtggatcaa ggtcaagcac gagcacacac aggaagtcat catcggcggc
     2581 tggaagcccg gcgaaggagg acgtgcaggg cgcatcggtt cgcttcttct cggaatcaat
     2641 aacgatgctg gcgagctgat ctacgtcggc catgtcggga ccgggttcac cgatcgcgtg
     2701 ctcgccgatc tggcgtcgaa gttggagcca ctgcaccgca agaccagtcc gttcaccgac
     2761 gaggtgccga ggctgcatgc ccgcgacgcc gtttgggtca cgccctcgct ggtcggtgag
     2821 gtcagctatg gcgaattcac ccgcgagcac cgactgcgcc atccgtcatg gcgagggctg
     2881 cgaccggaca agtcgccgac cgacgtccac cgcgagccgt agccgtccga ctcgagccct
     2941 agacttgcgc gggtgacatt cgagcttgca caggtgaaca tcggacggtt ggtggcgccg
     3001 ctcgaccacg cccagctcgc cgacttcgtc gcgcggctcg aacccgtgaa cgccgtcgca
     3061 gataacgccc ccggtttcgt gtggcgattg cagaccgacg acggcgacgc cacgtcgatc
     3121 cgtgccttcg aatgggacgc cggggacagc gccggcgtca tcgtgaacat gtcggtgtgg
     3181 accggtgtcg atgaactcgc ggccttcgtg ttcggcgaga tgcaccggga actgttgcgc
     3241 cggcgccgtg aatggttcca ccgcatgagc gaggcttaca cggtctgctg gtgggtgccc
     3301 gcaggccatc ggcctacgac cgacgaggcc gaggatcgca ttcgcaacct gcgcgccaac
     3361 ggcccgagcc ctgccgcctt cacgctaaaa gacaggtatc cgcgacccga cgagcacgtc
     3421 agtcatgcgt gagcgcgggg gcagtcgcta cagattcgcg tactgagcga gcatgacgag
     3481 cgcggcggct gccatcaagg cgaacgcgag ccagccaaga acgttcgaga tcttgccgtt
     3541 gcggtactcg cccatgatcg ccctgtcgcg tgcgaccacc atgacgacga acaagaaggg
     3601 cgcggcggcg atgccgttga ggatcgcgac gagcacgagc aggtggatcg ggttggtcga
     3661 tagcagcgac aggatcgtgc cgccgatggt gccgacggcc accagcccgt aaaacaccgg
     3721 cgcctttcgc accgaacggg agaatcccca gcgcttgccc aaaagcccag ccatgccggc
     3781 cgcacccgat cctgcaagga ccggaatggc gagcatgcca gtgccgatga aacccaacgc
     3841 gaacagcaca gacgaccaat ggcccgcaac cggcttcagc gcactggctg cgtcggcggc
     3901 gctgctaatg gctttgccgt gcagcgtggt tgccgtgacg acaatgatcg cgaacatcac
     3961 ggcgttcgaa aacgccatgc cggtgaagac gtcgccgcgc gcagtgcgcg acttgtactt
     4021 ggcgtctgcc gtgctccgat gggtcaacgg aacggcgtcg tcgccgcctg ctggctcggc
     4081 ccgcaactct tcgagccggt gtgccgattg ccagaagaac agataagggc tgatggtcgt
     4141 gccgaggacg gcgaccagca gcgcaaagta gctcttgctg agcgtgagat gcgggacgac
     4201 ggtgcgcagc ccgacctggc cccagttgac gtgaatcgag aaaagcacaa cgaagtaggt
     4261 caacaacgcc aagcagatga tcttgaatac gcgagcgatc tccgtgaacg agcccatgat
     4321 caccatcgcc gtgatcacga cgccggcaac aagggcccac acccaggtcg ggccggcgtg
     4381 cagcaggttc atgccactgc cgatagcaac gaggtcggca gagatgttca gcgcgttcgc
     4441 gacgatcagc gcactgatca gcacaatcag aacgggtctg gtccgcgcct tgaacttctc
     4501 agtggcgagc tcacctagcc cgcggcccgt cgccagtgcg gtgcggtcac agatttcctg
     4561 gacggcggcc atcagcggca acgtcaacag cgccacccac aacaagccga ggccgaactg
     4621 cgcgcccgct tgggagtacg tcgcgacacc cgaggggtcg tcgtccgagg cgccggtcac
     4681 caagccggga cccatcgctg tcaggtaccc gcgccacccg tgagaggctc ccgccttcgt
     4741 caggtcgacg tcggattgtt tgccgctgga cctgcccggc tcgccttggg atcggcgcgg
     4801 tttgccggtc ggcggccgct gcttgctttg agcacccgaa cggggcggcg acggtgttcg
     4861 ggccacggca cacatgtgcc cgccgccgac gacgtcaaac gtcgcggtag gtgtggatgg
     4921 tcacaggttt tcaatggtta cgagcctcct gcgatgggca tattcacacc cgatcgctcg
     4981 cggctgcgag tagacctcaa ggaggcactc cgatgatcgt gctcggcatc gtcttactca
     5041 ttgttggcat cgtccttaac gtcgggattc tctacacgtt gggcatcgtg ctcgtggtcg
     5101 ttggcgccgt gcttttcctg ctcggctcgg ctggacacgc agtcggtggc cgaagccact
     5161 actggtaaca atccctgcga agcaaggaaa tggcccttcc gtacagtcgt gaaaggtaag
     5221 gagtcgacat gcgcgtcgtc gcgatcatcg tcgtcgcctg gctgatcatc ggtatcgccg
     5281 ccaccgtgca gcg
//
LOCUS       DATCBJ010000386         3838 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_547453, whole genome shotgun sequence.
ACCESSION   DATCBJ010000386 DATCBJ010000000
VERSION     DATCBJ010000386.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 3838)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3838)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3838
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_547453"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..336
                     /locus_tag="VII50_07695"
     CDS             <1..336
                     /locus_tag="VII50_07695"
                     /inference="COORDINATES: protein motif:HMM:NF015155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HEY5050770.1"
                     /translation="VENRRLGMSTRDAVERGIASTAGVITSAAVIMVAVFSVFGTLSQ
                     VSMKQIGVGLAVAVLLDATIVRAVLLPSVMAMLGERNWYLPRWLQRLPLGRPIEVEPA
                     GQVEVLQEV"
     gene            complement(338..979)
                     /locus_tag="VII50_07700"
     CDS             complement(338..979)
                     /locus_tag="VII50_07700"
                     /inference="COORDINATES: protein motif:HMM:NF013933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family translocator"
                     /protein_id="HEY5050771.1"
                     /translation="MLTALVTFACAAALIVLLPGPDTLVVVRNLIRGGRRSAALTVIG
                     VLSGLAVWVTAAALGLAALLRASRDGYTALRLVGAVYLIWLGVNSLRTRAGTRNPATS
                     TPGEVQFPDATRRLVGTGYRAGLATDLLNPKVGVFFVSFLPGFVPAGHAVGPTSLLFG
                     AIFVVETAMYFALLLLLAARVTRWMSEPRVRRRLDRATGLVLIGFGVRIASER"
     gene            complement(1019..1810)
                     /locus_tag="VII50_07705"
     CDS             complement(1019..1810)
                     /locus_tag="VII50_07705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5050772.1"
                     /translation="MSDSGVSDGRLGIFIAVALLVIAIPGALVLREMSLPAPHPLGST
                     ASNSSFGPASAAAVDEAPDLSSPAPAVFPSSAPLSSAPLSSAPLSVPVVPLSLPAVPT
                     TLVGSPVAGITVGPAHATTDQAGHFSAVFPSRPVFHDIPASVGDTRYTMHIAVGQIDT
                     AVATSAEVVEETQINPGLPPSSFDANLRAVIGTFAGSNSRAALTSETSTTFQGEAARE
                     GQFSITDGLPLTVLAFMDGPNRLYVLSAPAGALFDALAASLVLLP"
     gene            complement(2228..2716)
                     /locus_tag="VII50_07710"
     CDS             complement(2228..2716)
                     /locus_tag="VII50_07710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5050773.1"
                     /translation="MESYFQFGRRTGSSATAFVVAALLIAAALMLGVGQIKSGSIERA
                     YRHTQHATGSVISVGENRIAEVRYLWAGKPQTGELDVSAASGVDSGDRLALRVSDGGR
                     QLQLETPFYTAVYRWTAVGLTAIALVVVLSSWRSAAGPTGEGKQWLPAELRRPRRRYR
                     VS"
     gene            complement(2719..3618)
                     /locus_tag="VII50_07715"
     CDS             complement(2719..3618)
                     /locus_tag="VII50_07715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5050774.1"
                     /translation="MTRRGLQITLGVLWLIDAGLQLQPYMFSTRFATEVLAPAGRSQA
                     SFVSAPVDHVSHIVGQHPVAFNVAFASLQLAIGVGLLLRRAVVPSLIASIVWSLTVWY
                     LGEGLGGLTGPNAALLLGAPGAALLYAVLAAAAWPDDSDDPDQQPKAWLAFAWAGYWI
                     GGAILQIWHGPRIGPDLAATVAEGANGAPGWLSRFDFSVARNLTHVSPAVVEGFIVLQ
                     VLIGLAVFGPARSRQVAAVVGSAICCGFWILGPGFSQFVSGHATDPNTAPLIVVLAVA
                     VVASASRPTMDTVVVSRRSKVAG"
     gene            complement(3640..>3838)
                     /locus_tag="VII50_07720"
     CDS             complement(3640..>3838)
                     /locus_tag="VII50_07720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5050775.1"
                     /translation="SPDMGGLSADGTQLWVSGRYNGVVYDISTVDGRLIREIKVGSGP
                     HGLCVYPQPGRYSLGHTGVFR"
BASE COUNT          764 a         1234 c         1245 g          595 t
ORIGIN      
        1 gtcgagaatc ggcggctagg catgagtacg cgggacgccg tggagcgggg catcgcttcg
       61 accgcaggcg tgatcactag tgcggccgtc atcatggtcg cggtgttctc ggtgttcggc
      121 actttgtcgc aagtctcgat gaagcagatt ggggtcgggc tcgcggtggc ggtgctgctc
      181 gacgcgacga tcgtgcgtgc tgtgctgctg ccatcggtga tggcgatgct cggcgagcgc
      241 aactggtacc tgccgcgttg gcttcagcgg ctgccactag gtcggccaat cgaggtggag
      301 ccggctgggc aggtggaggt gctacaggaa gtctgagtca gcgttcggag gcgattcgca
      361 cgccgaatcc gatcagcacg agaccggtcg cacgatccag ccggcgtcgt actcgcggct
      421 cgctcatcca cctggtgacg cgggccgcga gtaggagcaa caaggcgaag tacatcgcgg
      481 tctcgacgac aaagatggca ccaaagagca atgatgtcgg accaacggca tgcccggccg
      541 gcacgaagcc cggcaggaag ctcacgaaga aaacgccgac cttcggattg agcaggtcgg
      601 ttgccaaccc ggcgcggtag ccggtgccga cgagccgccg agtggcatcc ggaaactgca
      661 cctcgccggg cgtgctcgta gcggggttgc gcgttcccgc gcgcgtgcgc agggaattca
      721 cgcccagcca gatgaggtag accgcgccca ccaaacgcag cgcggtgtag ccgtcgcggc
      781 tggcccgtag caacgcggcc aaccccaggg ctgctgccgt gacccatacc gccaggcccg
      841 acagcacacc aataacggtg agtgcggccg accgtcgtcc gccccggatc aagttgcgga
      901 caacgacgag cgtgtcgggg ccgggaagca gcacgatgag tgcggccgca cacgcgaagg
      961 tcaccaacgc cgtcagcatg tgcgaatcat gacacgacgc gcgccgggac cgccgtgatt
     1021 atgggagcag cacgagactc gccgcaagcg cgtcgaagag tgcacctgcg ggtgctgaca
     1081 gcacgtacag cctgttcgga ccgtccatga atgccagcac cgtcaaaggt agcccgtcgg
     1141 tgattgagaa ctgaccttca cgggcggcct cgccctggaa tgtcgtcgac gtttcggatg
     1201 tcagcgcggc gcgggagttg ctgccggcga acgtgccgat cacggcacga aggttggcat
     1261 cgaaggacga cggtggcagc ccagggttga tctgggtctc ctcgaccacc tcagctgagg
     1321 tcgcaactgc ggtgtctatc tgcccgaccg caatatgcat cgtgtaacga gtgtcgccca
     1381 ctgaagctgg tatgtcatgg aagaccggcc tggacgggaa cacggccgaa aagtgacctg
     1441 cctgatcggt tgttgcgtgc gctgggccga ccgttatccc ggcaaccggt gagccaacga
     1501 gcgtggtggg cacagccggc agggatagtg gcacaaccgg caccgacagg ggtgccgacg
     1561 acaggggtgc cgacgacagt ggtgccgacg acggaaagac ggccggggct ggcgaactga
     1621 ggtccggtgc ctcatcgacc gccgcggcac tcgcggggcc aaacgagcta ttgctcgcag
     1681 tcgaacccaa ggggtgcggg gccggtagcg acatttcacg cagcactaac gcgccgggta
     1741 tcgcgatgac cagcagagcc accgcgataa agatcccgag acgaccatca gatactccgc
     1801 tgtcgctcaa cgaggggccc ctcactaccg ggatccggct cagcgcctcg acggtgccgc
     1861 tgatgctacg ggtcgcagtc ccaggcgcct actcgactga cctgtctgag ttttgcaaga
     1921 cttgaacgtt aggtttcatg atctacataa ctgtttgcgt cctgactcca aagcgacgcg
     1981 actgctctcg gcgtcatcgc tgaactacga gccaggcgcg tcgacgtccc gcgtgatgtt
     2041 gctgttgcag gcttcgacga caccgcactt gcccgctacg ccggtctcac cacggcgaca
     2101 catccagtgg aggcgattgc cgaggcggcg acccgcgccg tgctcgacgt gcgtgtgacg
     2161 cgaccgcctg acatgtactt cccgtcgcag ctcgtcgttc gccaaaccgc gtgaagccta
     2221 gcgccggcta gctgacgcga tatcgccgac gagggcgccg cagctcagca ggcaaccatt
     2281 gcttgccctc cccagttggg cccgcggctg agcgccacga cgacaacacc actacgagcg
     2341 cgatcgctgt cagaccaact gccgtccacc tgtacaccgc cgtgtagaac ggcgtttcca
     2401 actgcaactg gcgcccaccg tcggagacgc gtaacgcgag ccgatcaccg ctgtccactc
     2461 ccgacgccgc gctgacgtcc agctcccctg tctggggctt gccggcccac aggtagcgaa
     2521 cctcggcgat ccggttctcg ccgacactga tcacggatcc agtcgcgtgc tgggtgtgcc
     2581 ggtaggcgcg ctcgatcgaa ccgctcttga tctggcccac accaagcatg agcgccgcgg
     2641 caatcagcag agcggcaaca acaaacgccg tagcggacga acctgttcgc cgtccgaact
     2701 ggaagtagct ctccatagct atcctgcgac cttagaacgt ctcgagacga caaccgtgtc
     2761 catcgtagga cgggacgcgc tggcaacaac tgcgacggca aggacgacga tcagtggcgc
     2821 ggtgttcgga tcggtggcgt gtccgctgac aaattggctg aatcctgggc cgaggatcca
     2881 gaacccgcag cagatggcgg acccgacgac cgcggcgacc tggcgagacc gcgcggggcc
     2941 gaatacggcg agcccgatga gcacttggag cacgatgaag ccctcgacga cggcgggact
     3001 cacatgcgta aggtttcggg ccaccgagaa atcgaagcgg ctgagccagc caggggcacc
     3061 gttagcgcct tctgcgactg tggcggccag atcgggaccg atccgcgggc catgccatat
     3121 ctgcaagatg gccccaccga tccagtagcc cgcccaggcg aatgcgagcc aagccttcgg
     3181 ctgctggtcc gggtcgtccg agtcgtcggg ccacgccgcc gccgccagca ccgcatacag
     3241 caatgcggcg ccgggtgcac ccaagagcaa agctgcattg ggcccggtca ggccacccaa
     3301 gccctcaccc aggtaccaga ccgtcaggga ccacacgatc gaggcgatga gcgacgggac
     3361 gaccgcgcgg cgcagcagta gacctacgcc gattgcaagc tgcagcgatg cgaacgcgac
     3421 attgaatgca actgggtgtt gaccaacgat gtgcgaaacg tgatcgacgg gtgctgaaac
     3481 gaacgacgct tgactgcggc cagcaggagc gagcacttcg gtcgcgaacc gggtactgaa
     3541 catgtacggc tggagctgca agccggcatc gatcaaccac agaacgccca acgtgatctg
     3601 cagacctcgc cgggtcattg cacacaactc cgctcagggc tagcggaaaa ctccggtatg
     3661 acccaaggag taccggccgg gttggggata cacgcatagc ccgtgcggtc cgctgcctac
     3721 cttgatctct ctgatcaacc gcccgtcgac ggtactgatg tcatagacaa ctccgttgta
     3781 gcgtcccgag acccacaact gcgtgccgtc cgcagacagg ccacccatgt cgggcgag
//
LOCUS       DATCBJ010000387         2218 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_356813, whole genome shotgun sequence.
ACCESSION   DATCBJ010000387 DATCBJ010000000
VERSION     DATCBJ010000387.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2218)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2218)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2218
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_356813"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            232..1182
                     /gene="deoC"
                     /locus_tag="VII50_07725"
     CDS             232..1182
                     /gene="deoC"
                     /locus_tag="VII50_07725"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020633497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HEY5050776.1"
                     /translation="MTLTTSAIPTDVASSDSALRRFLHGLPGVDQVGAQARAAALSTR
                     SIKTDAKRWAIDTAISMIDLTTLEGQDTHGKVRALCAKGIRPDPSDASCPSVAAICVY
                     PDLVSTAKEYLGDSGVKVASVATSFPSGRAALTVKLADVRDAVTNGADEIDMVIDRGA
                     FLEGHYLDVYDEIVQVKAACKGAHLKVILETGELATYDNVKRASWLAMIAGGDFIKTS
                     TGKVSPAATLPVTLVMLQAVRDFRDTTGRQVGVKPAGGIRTTKDAMRYLVLVSETVGD
                     DWLSPDWFRLGASSLLNDLLQQRQKLATGHYSGPDYVTID"
     gene            1193..>2218
                     /locus_tag="VII50_07730"
     CDS             1193..>2218
                     /locus_tag="VII50_07730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009072622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEY5050777.1"
                     /translation="MTSKLFPYSPAPESRAIVNIASTYGLFIGGEFVESIDGDEFKSV
                     NPATEEVLAEVVDAGPRDIDRAVAAARKAYDGVWGAMPGKERSKYLFRIARIIQERSR
                     ELAVLESLDNGKPIKESRDVDLPLVAAHFFYYAGWADKLEHAGFGANPKPIGVAAQVI
                     PWNFPLMMLAWKIAPALACGNTVVLKPAETTPLTALVFAQICQQAGLPAGVVNIVTGA
                     GDTGKTLVGHDGVDKVAFTGSTEVGRAIAKTLAGTRKRLSLELGGKAANIVFDDAPID
                     EAVEGIVGGIFFNQGHVCCAGSRLLVQESVADEVLDRLRRRMSTLRLGDPLDKNTDVG
                     AINSSAQL"
BASE COUNT          402 a          760 c          700 g          356 t
ORIGIN      
        1 agccggtctc gtagccgagc tgcccgaggt gggtgacgac gtttgccacg aggcgctcga
       61 gcgcgccgct ggagatgcgg gtctgcgatc ccgttggggt gcgcgcgagc cgccgcaggc
      121 cagccttcac gtgacccctc cgccgtcgtc agttcatagt cccagccgca tccgctcagg
      181 tcgcgcaact cgcccgaagc caaccgcgcg gcgcggcgat atggtgaatc aatgacgctc
      241 acaacgtcgg cgatcccgac agacgtcgct agttccgact ccgccttgcg ccggttcctg
      301 cacggtttgc ccggtgtcga ccaagtcggt gcccaggccc gggcagcggc gctgtctacc
      361 cgctcgatca agaccgacgc caagcgctgg gcgatcgaca cggccatctc gatgatcgac
      421 ctgacgacac ttgagggcca ggacacccac ggcaaggtcc gcgcgctctg cgcgaagggc
      481 atccgccccg acccgtccga cgccagctgc ccgagcgtcg cggcgatctg cgtctatccc
      541 gacctcgtct ccaccgccaa ggagtacctc ggcgacagcg gcgtcaaggt cgccagcgtg
      601 gcgaccagct tcccgtccgg ccgggctgca ctgaccgtca agctcgccga cgtccgggac
      661 gcggtcacga atggtgccga cgagatcgac atggtgatcg accgcggcgc gtttctcgaa
      721 ggccattacc tcgacgtgta cgacgagatc gtgcaggtga aggccgcgtg caaaggcgcc
      781 catctcaagg tgatcctcga aactggtgag ctcgcgacct acgacaacgt gaagcgcgca
      841 tcgtggctgg cgatgatcgc gggcggcgac ttcatcaaga ccagcaccgg caaggtcagt
      901 ccggccgcga ccctgccggt caccctcgtg atgctgcaag ccgtgcgcga cttccgcgac
      961 acgaccggaa ggcaagtagg cgtcaagccc gcgggcggca ttcgcaccac gaaggacgcg
     1021 atgcgctacc tggtgctcgt gagcgaaaca gtcggcgatg attggctcag ccccgactgg
     1081 ttccgtcttg gtgcgtcgag cttgctcaac gacctcctcc agcaacgcca gaagctggca
     1141 accggccatt actcgggccc cgactacgtg acgatcgact gaaggcgcgc acatgacgag
     1201 caagctgttc ccgtattcgc cggcacccga gtcgcgggcg atcgtcaaca tcgccagcac
     1261 ctacggtctg ttcatcggcg gggagttcgt cgagtcgatc gacggcgatg agttcaaaag
     1321 cgtcaacccg gcgaccgaag aggtactcgc cgaggtcgtc gacgccggcc cgcgcgacat
     1381 cgaccgcgcg gttgccgcag ctcgcaaggc ctacgacggc gtctggggcg cgatgcctgg
     1441 caaggagcgc tcgaaatatc tgttccgcat cgcccgcatc attcaggagc gctcgcgcga
     1501 gctcgcggta ctcgaatcac tcgacaacgg caaaccgatc aaagaatcgc gggacgtcga
     1561 tctgccgctc gtcgcggctc acttcttcta ttacgcgggc tgggccgaca agctcgagca
     1621 cgcagggttt ggtgccaacc cgaagccgat cggcgtagcc gctcaggtca tcccgtggaa
     1681 cttcccgctg atgatgctcg cctggaagat cgctcccgcg ctcgcctgcg gcaacaccgt
     1741 cgtgctgaag cccgcagaga cgacgccatt aaccgcgctg gtgttcgcgc agatctgcca
     1801 gcaggccggc ctccccgctg gcgtcgtgaa cattgtcacg ggtgccggcg acaccggtaa
     1861 gacgctggtc ggccacgacg gcgtcgacaa ggtcgccttt actggctcga ccgaggtcgg
     1921 ccgagcaatc gcgaaaaccc ttgctgggac aagaaaacgg ctcagcctcg aactcggcgg
     1981 caaagcggcc aacatcgtgt tcgacgacgc accgatcgac gaagccgtcg agggcatcgt
     2041 cggcggcatc ttcttcaacc agggtcacgt gtgctgcgcc ggctcacgac tgctcgtgca
     2101 ggagtcggtc gccgatgagg tgctcgaccg gttgcgccgg cggatgtcga ccctgcgcct
     2161 cggcgatccg ctcgacaaga acaccgatgt cggcgcgatc aactcctccg ctcagctt
//
LOCUS       DATCBJ010000388         2000 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_445158, whole genome shotgun sequence.
ACCESSION   DATCBJ010000388 DATCBJ010000000
VERSION     DATCBJ010000388.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2000)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2000)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2000
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_445158"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..376)
                     /locus_tag="VII50_07735"
     CDS             complement(<1..376)
                     /locus_tag="VII50_07735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5050778.1"
                     /translation="MTEYNSESASVLTAVRDGFAPVTMHTPLDAVVVQAGARRRRRRA
                     MTVTAVTATATATALAVGAVGYGQGSTRSAKSATPSTAAQPVHIVTAAYTVDSRPNGS
                     VTVTWTKQGYFQDPSGLQAALQK"
     gene            complement(373..894)
                     /locus_tag="VII50_07740"
     CDS             complement(373..894)
                     /locus_tag="VII50_07740"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEY5050779.1"
                     /translation="MPVLWRSRGVEQLEFADFYVDSRDVCLRAVLASVVDRSLAEDLV
                     AEAFTRALMSWPKVSQHPAPRAWVVRTALNARVSWWRKRRKEVPLGITAYMTAVAEPV
                     IEVDPGLLRVLRQLPRRQREVIAVRVFLDLDTRGTAELLGISEGTVKAHLSRAVATLR
                     VQLINHHQAEVLP"
     gene            complement(944..1303)
                     /locus_tag="VII50_07745"
     CDS             complement(944..1303)
                     /locus_tag="VII50_07745"
                     /inference="COORDINATES: protein motif:HMM:NF015735.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HEY5050780.1"
                     /translation="MAKFLVLIYGREQRWDAMSADEMRAHDAAHVEFSKAAGKKIISG
                     EELESPSKARTLRRRHSARTAAATDGPFVETKEILGGYYLLEAENLDEVEALVALLPE
                     VQADHSGVEIRPVVDHG"
     gene            complement(1421..1861)
                     /locus_tag="VII50_07750"
     CDS             complement(1421..1861)
                     /locus_tag="VII50_07750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5050781.1"
                     /translation="MPVGFDPPTSLATDQFHLEPLGPQHNQADHAAWMSSIEHIRSTP
                     GYPDGNWPPLTGMTLERNLADLRRHADDFSRGVGFTFTVLDPDDNDVIGCVYLYPSAS
                     EKWDVTVQSWVRADRSALDRPLADAVAGWLATDWLWVRVDRCGR"
BASE COUNT          354 a          646 c          632 g          368 t
ORIGIN      
        1 ccttctgcag tgccgcttgc aagccggacg ggtcttggaa ataaccctgc ttcgtccacg
       61 tcaccgtgac cgagccgttc ggtcggctat cgaccgtgta cgccgcggtg acgatgtgca
      121 cgggttgtgc agcagtcgaa ggcgtggctg acttcgcaga gcgcgtcgat ccttggccgt
      181 agccaactgc tccgaccgcc agagcggtcg cggtcgcggt cgcggtcacg gccgtgacgg
      241 tcatcgcgcg gcggcgacgc cgacgggcgc ccgcctgaac gacaaccgcg tcgagcgggg
      301 tgtgcatcgt gacaggtgcg aacccatcgc gtaccgcggt cagcaccgat gctgactcgg
      361 agttgtactc ggtcatggca aaacctccgc ctggtggtgg ttgatgagtt gaacgcgcag
      421 tgtcgcgacg gcgcgtgaaa ggtgcgcttt gacggttccc tcggagatcc ccaagagttc
      481 ggcggtgccc cgagtgtcga gatcaaggaa cacccgcacc gcgatgactt ctcgctgccg
      541 acgaggcagt tgacgcaaca cccgcagcag gccgggatca acctcaatca ccggttcggc
      601 gacggcggtc atgtaggcgg tgatgcccag cggcacctct ttgcgacgtt tgcgccacca
      661 ggacacccgt gcgttgagtg ccgtgcgcac gacccacgcg cgcggcgccg ggtgttgact
      721 caccttcggc cacgacatca acgcgcgggt gaacgcctcg gccaccaagt cctcggcgag
      781 gctgcgatct acgacgctag ccagcactgc ccgcaggcaa acatcccggc tatctacgta
      841 gaagtccgcg aactccagtt gctccacacc cctactacgc cacagcaccg gcatccgttt
      901 acaccgttca cgaaccatgt caccggtcaa tggtggtcgg gcatcacccg tggtcgacga
      961 ccggccggat ctcgactcca ctgtggtcag cctgtacctc aggcagcaat gcgacgagcg
     1021 cctcgacctc gtcgagattt tctgcctcaa ggaggtagta accgccgagg atctccttcg
     1081 tctcaacgaa cggcccgtca gtcgcagcgg cggtgcgagc tgagtggcga cggcgcaacg
     1141 tcctcgcctt cgacggcgat tcaagctcct cgccactgat gattttcttg cctgcggcct
     1201 tgctgaattc gacgtgcgca gcgtcatgag cgcgcatctc gtcagcggac atcgcgtccc
     1261 agcgctgttc gcggccgtag atcaagacca gaaacttcgc catgtcttcg tcctgtcagc
     1321 tcgagcccgg ctgggccttc tagttggacg acgaatcgtg ccgctcgcag atcgacattt
     1381 gtgacaacaa tgtgttggcc cggttcgccg cgatcacgac tcagcgaccg cagcgatcca
     1441 cacgcaccca tagccagtcg gtggcgagcc agcctgcgac ggcgtcggcg agtggccggt
     1501 cgagcgccga tctgtcggcc cgcacccacg actgcactgt gacgtcccac ttctcggagg
     1561 ctgagggata caggtacacg cagccgatca cgtcgttgtc atctgggtcg aggacggtga
     1621 aggtgaagcc gacgccccgg ctgaagtcgt cggcgtggcg ccgaagatcg gcaaggttgc
     1681 gctcgagcgt cataccagtg agtggtggcc agttgccgtc gggatagccg ggagtcgagc
     1741 gaatgtgctc gatgctcgac atccaggcgg catgatcggc ctgattgtgc tgcggtccca
     1801 acggttcgag gtggaactgg tcagtggcca gtgacgtcgg cggatcgaat ccgactggca
     1861 cgaacgcatc cgcggtcatg accgagacgg taacgggctg gcctgtcttt ctgcactcga
     1921 gttgttctcc ccctgtagca catgcgctac catggctcca tgacgacgac tgggttgcgt
     1981 gagctccgtc agcaggccag
//
LOCUS       DATCBJ010000389         3927 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_86420, whole genome shotgun sequence.
ACCESSION   DATCBJ010000389 DATCBJ010000000
VERSION     DATCBJ010000389.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 3927)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3927)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3927
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_86420"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..319)
                     /gene="murD"
                     /locus_tag="VII50_07755"
     CDS             complement(<1..319)
                     /gene="murD"
                     /locus_tag="VII50_07755"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719844.1"
                     /note="UDP-N-acetylmuramoylalanine--D-glutamate ligase;
                     involved in peptidoglycan biosynthesis; cytoplasmic;
                     catalyzes the addition of glutamate to the nucleotide
                     precursor UDP-N-acetylmuramoyl-L-alanine during cell wall
                     formation; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HEY5050782.1"
                     /translation="MAEVIPSWLPTAAHDAPWHELTVCVAGVGVSGAACARVLRMLGA
                     GVIAVDAGASEREQQAAISLHSLGVDVRLGDGETLPPGAQLVVTSPGWRPESPLFVAA
                     ATSG"
     gene            complement(307..1368)
                     /gene="mraY"
                     /locus_tag="VII50_07760"
     CDS             complement(307..1368)
                     /gene="mraY"
                     /locus_tag="VII50_07760"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HEY5050783.1"
                     /translation="MKGILAAGTIALIVSLFGTPFAIRLLRKRGYGQLIRDDGPTTHH
                     TKRGTPTMGGTVILLAAILGYAVGNMVSASGPTASGLLVLGLATGLGLVGFLDDFIKV
                     RMQRNLGLRARTKLLGQTLVAAVFGVLVLQFPDSSGIHPASTHISFVRDTTVALGAVG
                     FVVWALVMVSSTSNGVNLTDGLDGLATGASTMIFAAFVIMTFWQGRNDCLTKAAAGCY
                     PVRDPFDLAILAAAFAGSCAGFLWWNAAPARIFMGDTGSLALGGALAGMAIMTRTELL
                     LAVLGGLFVVITMSSIIQIGYFKMTHRRVFKMAPLQHHFELKGWAEVTIVIRFWIIAG
                     LAVALGLGVFYAEWITWQK"
     gene            complement(1365..2777)
                     /gene="murF"
                     /locus_tag="VII50_07765"
     CDS             complement(1365..2777)
                     /gene="murF"
                     /locus_tag="VII50_07765"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HEY5050784.1"
                     /translation="MTLTVGEIAQLSHGVVSDLADPNVHVIGPVVVDSRRVTRGCLFV
                     AVSGARVDGHDFAIQAVADGAPIVLASKPVPVPAVVVGDTVRALGDLARGYLHRLANP
                     VVVGITGSSGKTSTKDIVGQLLRDLGPTVAPEGSFNTEVGLPLTVLRADADTRYLVLE
                     YSARGLDHIAYLCGIARPRIAVVLNVGAAHLGEFGSRTAVAKAKGELIEALPDDGLAV
                     LNADDDAVAAMASLTGARLVTFGLGAHADVRAVDVALDELARPTFRMLTAIGEVEVSL
                     PLHGAHHVSNALAAAAVALELGFDLESVAAALARIEAISRWRMQVSERADGLVVINDA
                     YNANPESMRAALAALAAIGSGARHRRTHAVLGTMAELGPAEATAHRELGAAAALIGVH
                     QLVVVGAAAKGIADGAIEAGLEPARVNVVDGVADAVSVLERDLGAGDVVLVKASRSAD
                     LQRVAEALLAGPDSQAGATR"
     gene            complement(2777..>3927)
                     /locus_tag="VII50_07770"
     CDS             complement(2777..>3927)
                     /locus_tag="VII50_07770"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: protein motif:HMM:NF001126.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HEY5050785.1"
                     /translation="SYLLDAGLRAAGDKTGLVGTIETRIGEWVAPSARTTPEAPDLQA
                     LLAVMAERGVGSVSMEVSSHALALHRVDGTAFDVVAFSNLSQDHLDFHADLDDYFNAK
                     ARLFTSRFARAGVVNVDDEHGRRLLGVADIPLTTVSATSRDAQWWASDESYASGSGEQ
                     RTDFVVHGPELVTAVSIGLPGSFNVANALLAFVTLVVAGVDPRAAADGIAAVSVVPGR
                     MEAVLMSQPFAALVDYAHTPDAVVTLLTTLRATTSGRLIVVLGCGGDRDRAKRPLMGA
                     AAARLADVAVLTSDNPRSEEPDQILAAMLGGAMGVAPAQRAEVIVELDRKTAIQIAVS
                     QARAGDVVVVAGKGHEQGQEVGGVVHPFDDRTVLRAELAALTNGAGAS"
BASE COUNT          762 a         1341 c         1234 g          590 t
ORIGIN      
        1 cgccggatgt cgccgccgcc acgaacaacg gtgattcggg tcgccagcca ggcgatgtca
       61 cgacgagttg agcgccgggt ggcagcgtct ccccgtcacc aagccgcaca tcgacaccga
      121 gcgaatgcaa cgagatcgcg gcctgctgtt ctctctcgct cgcaccggca tcgaccgcaa
      181 tcacgcccgc gcccagcatg cgcaacaccc gcgcacaggc agcaccggat acaccgacgc
      241 cggcaacaca gaccgtcaac tcgtgccaag gcgcatcatg ggcagctgtg ggcaaccacg
      301 acgggatcac ttctgccacg tgatccactc ggcatagaaa acgccgaggc cgagagcgac
      361 ggcgaggccc gcgatgatcc agaatcgaat cacgatggtc acttcggccc aacccttgag
      421 ctcgaaatga tgctgcagcg gcgccatctt gaagacgcgg cgatgcgtca ttttgaagta
      481 gccgatctgg atgatgctcg acatcgtgat gacgacgaac agtccaccga gcaccgcgag
      541 taacaactcg gtacgcgtca tgatggccat gcccgcaagg gcgcccccaa gcgcgagtga
      601 gccagtgtcg cccatgaaga tgcgggccgg tgcggcgttc caccacagaa agcctgcgca
      661 cgaacccgca aaggcggcgg cgaggatcgc tagatcgaat gggtcgcgga cgggatagca
      721 acctgccgcg gctttggtca ggcagtcgtt gcggccttgc cagaacgtca tgatgacgaa
      781 tgccgcaaag atcatcgtgg aggcgccggt cgcgagaccg tcgagtccgt ctgtcagatt
      841 caccccgttc gatgtcgacg acaccatcac cagcgcccac acgacaaagc cgacggcacc
      901 gagcgcgacc gtcgtgtcgc gcacgaacga gatgtgggtc gaggcgggat gaatacctga
      961 gctgtcgggg aactgcaaca caagaacgcc gaagacggct gcgacaagtg tctgcccgag
     1021 tagcttcgtg cgtgctcgaa gaccaagatt gcgctgcatc cggaccttga tgaagtcgtc
     1081 gagaaaccca accagaccaa ggccagtcgc gagtccgagc accagcaggc cggacgcggt
     1141 cgggccgctc gcactcacca tgttgccgac cgcgtagccg agaatggcgg ccagcaagat
     1201 gacggtgccc cccatggtcg gggtgccgcg cttcgtgtga tgcgtcgttg gcccgtcatc
     1261 gcggatcaac tgcccgtagc cccgcttgcg cagcagccgg atcgcaaacg gcgtaccgaa
     1321 cagcgacacg atcaacgcga tcgtgcccgc ggccaggatc cccttcaccg ggttgcccca
     1381 gcttggctat caggaccggc aagcaaggct tcggcgactc gctgaaggtc ggccgagcgt
     1441 gacgccttca ccagcaccac atcgcccgca ccaaggtctc tttcgaggac agacacggcg
     1501 tcggcaaccc catcgacgac gttcacgcgc gcgggttcga gccccgcctc gattgcaccg
     1561 tcggcgatgc ccttggccgc cgcacccacg acgacaagtt ggtggacgcc gatcagcgcc
     1621 gccgcagcac caagctcgcg atgcgccgtc gcctccgccg ggccgagttc cgccatcgtc
     1681 ccaagaacag cgtgggttcg acggtgacgt gcgccggagc caatggccgc caacgccgct
     1741 agcgcggctc tcatcgattc cggattcgcg ttgtacgcgt cgttgatgac gacgagaccg
     1801 tcagcgcgtt cggacacctg catccgccag cgactgattg cttcgatccg cgccaatgcg
     1861 gcagccactg actcgaggtc gaagccaagc tcgagggcca cggcagcggc ggcgagcgcg
     1921 ttcgacacgt ggtgcgcgcc gtgcaacggc agcgaaacct cgacctcgcc aatcgcagtg
     1981 agcatgcgaa atgttgggcg agccaactcg tcgagcgcaa cgtcgacggc tcgaacgtcg
     2041 gcgtgtgcac cgagaccaaa cgtcacgagg cgcgcgccgg ttagcgatgc catcgctgcg
     2101 acggcatcgt catcagcgtt gagcacagcg aggccatcgt ccggaagggc ctcgataagt
     2161 tcacccttgg ccttcgccac cgcggttcgg ctaccaaact cgccaaggtg cgcggcgccg
     2221 acgttgagga cgacagcgat gcgtggcctc gcgatcccgc agaggtacgc aatgtgatcg
     2281 aggccacgcg cgctgtattc gagcaccagg tatctcgtgt ctgcatcagc ccgcagcacg
     2341 gtgagcggca gccccacctc ggtgttgaac gatccctcgg gggccaccgt cggcccgagg
     2401 tcgcgcagca attggccgac gatgtccttc gtgctggtct tgccgctgga cccggtgatg
     2461 cccacaacca cgggattcgc caaccggtgg aggtagccgc gcgcgaggtc accgagggcg
     2521 cgcaccgtgt cgccgacgac gacggccggc actggcacgg gcttgctcgc cagcacgatc
     2581 ggtgcaccgt cggccaccgc ctggattgcg aagtcgtgcc cgtccacacg tgctcccgaa
     2641 accgcaacga aaagacaccc ccgagtcacc cgacgcgaat caacgacgac cgggccgatg
     2701 acgtgcacgt tgggatcagc aagatccgac accacgccgt ggctcagttg tgcgatctcg
     2761 ccgaccgtga gggtcatcat gaagcgccgg cgccgttggt caacgcagcg agttcggcgc
     2821 gcaggacggt gcggtcgtcg aacgggtgca ccacgccgcc gacctcctgc ccttgttcat
     2881 gacccttacc tgccacgaca acgacgtcac cggcccgggc ttgactgaca gcgatctgga
     2941 tcgcggtctt cctgtcgagt tcgacgatca cctccgcgcg ttgcgccggg gcgactccca
     3001 tcgcaccacc gagcatcgcg gcgaggatct ggtccggttc ttcgctgcgc ggattgtcgc
     3061 tcgtcagcac cgcgacatcg gcgaggcgcg cggcggccgc gcccatcagt ggccgtttcg
     3121 ctcggtcgcg gtcaccaccg cagccaagga cgacgatgag ccggcccgag gtcgtcgcac
     3181 gcaatgtcgt gagcagcgtc acgaccgcgt caggggtgtg cgcatagtcg acgagggccg
     3241 caaacggctg cgacatcaag acggcttcca tgcggcccgg cacgacggac acggccgcga
     3301 tgccgtcggc tgccgctcgc ggatcgactc ccgcgacaac cagggtgaca aacgccagga
     3361 gtgcgttcgc gacgttgaac gacccaggaa gcccaatcga aactgccgta acgagctcag
     3421 gaccgtgcac gacgaaatcg gtgcgctgct cgcccgaccc actcgcgtac gactcgtcac
     3481 tcgcccacca ttgagcgtcg cggctcgttg ccgagaccgt ggtcagcgga atgtccgcaa
     3541 cgccaagcaa tcggcgtcca tgctcgtcgt ctacgttgac cacacccgca cgagcgaaac
     3601 gcgaggtgaa cagtcgcgcc ttcgcgttga agtagtcgtc gaggtcggca tggaaatcga
     3661 gatggtcttg agacaggttg ctgaaggcga ccacgtcgaa tgcggtgccg tcgacccggt
     3721 gcaacgcgag cgcgtgactg gagacctcca tcgagacgga cccgaccccg cgctccgcca
     3781 tgaccgccag cagtgcctgc agatctggag cctccggtgt cgtgcgcgca ctgggggcaa
     3841 cccactcccc gatcctggtc tcgatggtcc ccaccaggcc ggtcttatct cccgcggcac
     3901 gtagtccggc atcaagcagg tacgacg
//
LOCUS       DATCBJ010000390         7043 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_35188, whole genome shotgun sequence.
ACCESSION   DATCBJ010000390 DATCBJ010000000
VERSION     DATCBJ010000390.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 7043)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7043)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7043
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_35188"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..494
                     /locus_tag="VII50_07775"
     CDS             <1..494
                     /locus_tag="VII50_07775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5050786.1"
                     /translation="GEIEIAGTELGVAAGLAEIHPDWWRQRVRLDWVRAEIALLGEDP
                     TAAQEAASEAIDAAELAHAPRHVAKGLLFLGVAQAHLGSPDTVTTLRRAATLAEQLGT
                     TPLIWQARALVGALLAEDDPTTSARSLQAARSAVLAIAGDLPDDVREEWLNRPDIEAL
                     LSI"
     gene            651..1259
                     /locus_tag="VII50_07780"
     CDS             651..1259
                     /locus_tag="VII50_07780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019431595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEY5050787.1"
                     /translation="MTTVLICDDHRRFREHLRRYVAAVPGVDRVMTADTSAETFVRYA
                     AELPALVLLDVAMPDLGGPETARLLVSAHPGANVVMLAGTADSEGLARAMAGGARGYL
                     TKDVSVEELCAALAHVVVDAGVARANNGHGPAQIIDPPLTERERQVLDGMSRGKTNNE
                     IGRELFLSEDTVKTHARRLFRKLGVNDRAQAVALGFRSGIVR"
     gene            complement(1287..1721)
                     /locus_tag="VII50_07785"
     CDS             complement(1287..1721)
                     /locus_tag="VII50_07785"
                     /inference="COORDINATES: protein motif:HMM:NF036411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5319 family protein"
                     /protein_id="HEY5050788.1"
                     /translation="MPVPLSRRRPLAGTPSGRCAARLSRMHEATSEPLSPQDREEILV
                     DLADLEVFQTLLEPRGIRGLVVDCGECEAPHYFAWELLRQNLRQLLDVGQLRAHEPAF
                     EPDPADYVAWDYASGFVDGVLSESERDDGRDADDGPPTIDLR"
     gene            1836..3296
                     /gene="guaB"
                     /locus_tag="VII50_07790"
     CDS             1836..3296
                     /gene="guaB"
                     /locus_tag="VII50_07790"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007031114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HEY5050789.1"
                     /translation="MIGLTYDDVLLLPAASDVIPSQVDTTTAITRNISLRVPLLSSAM
                     DTVTEARMAIAMARQGGVGVLHRNLSIDEQAAQADLVKRSEAGMITNPVTCSADDTLA
                     DVDRLCSHYRISGAPVVDAAGVLVGIVTNRDIRFETDLSRPVRDVMTAMPLVTAPVGV
                     SDDDALDLLRRHKIEKLPLVDGVGRLRGLITVKDFTKREQFPDASKDAAGRLLVAAAV
                     GTGEDSYKRARALVEAGVDIIVVDTAHGHSSGVLEMVSRLKRDVSVDVIGGNIATREG
                     AQALVDAGADGVKVGVGPGSICTTRVVTGVGVPQVTAIYEAAEACRAAGIPVIGDGGL
                     QYSGDIAKAIAVGADAVMLGSLLAGVEESPGELIFINGKQYKSYRGMGSLAAMQSRGE
                     VRSYSKDRYFQDDVLSDDKLVPEGIEGQVPYRGPLAAVVHQLVGGLRSAMGYCGAATI
                     DQLQQARLVRITAAGLKESHPHDVQMTTEAPNYYSR"
     gene            3324..4433
                     /locus_tag="VII50_07795"
     CDS             3324..4433
                     /locus_tag="VII50_07795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GuaB3 family IMP dehydrogenase-related protein"
                     /protein_id="HEY5050790.1"
                     /translation="MAEVQIGMGKTGRRAYGFDDVAIVPSRRTRDPEEISVAWQIDAY
                     RFEAPIVSSAMDSVVSPDTAIEIGRLGGLGVLDLEGLWTRYDDPQPLLDEIATMPEAD
                     VVRRMREIYAEPMKEELIGARIKQIRDGGVVTAAALSPQRTAQYHKAVLEAGVDLFVI
                     RGTTVSAEHVSSRAEPLNLKRFIYDLDVPVIVGGSYTYQAALHLMRTGAAGVLVGVGG
                     GAAHTTRQVLGIGVPMATVVSDAAAARRDYMDESGGRYVHVIADGAMRTGGDIAKAIA
                     CGADAVMIGLPLARAAEAPGKGWHWGAEARHNDLARGQRFHAGTAGSLREVLYGPSTD
                     AEGTTNLVGALRKAMATCGYSDLKEFQRVEVVVTC"
     gene            complement(4430..5473)
                     /locus_tag="VII50_07800"
     CDS             complement(4430..5473)
                     /locus_tag="VII50_07800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011718987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEY5050791.1"
                     /translation="MEYRQLGDSGLFVSSLTLGSVAFTPGPGVAAATVDLDDARRLLE
                     LAIDAGVNMVDTADSYGNGVAEEVVGELLNGLPEVLVSSKVAFPMGPTPNDRGLSRRH
                     VLASADNSLRRLQRDHIDLYHLHLWDGLTRVEETLDALAFLVRSGKIRYVAVSNFSAW
                     QVMKLLAAADVMGAPRPIAQQIHYTPHVRDAEFELLPLAIDQGLGTLVWSPLAGGLLS
                     GKYRRNQPPPAGGRFASAFSEPPVPDEVRLYDIIDEIISVAEERGATPAQVALAYTLS
                     RPGVTSAIVGARRPEQLLDNLGSAELKLRDDDIGRIERVSRPVVPYPYWHQLTWDVER
                     LGPAERSILGPYC"
     gene            complement(5495..>7043)
                     /locus_tag="VII50_07805"
     CDS             complement(5495..>7043)
                     /locus_tag="VII50_07805"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEY5050792.1"
                     /translation="DVVTEDIGTDPDWAEWRALALEHGLLACWSVPVIARDHDGHDGQ
                     VLGTFATYFEATRGPSDDERRDVARTTALATIALARHRDEGELRRANLRDPLTGLAHR
                     RLFIDELGAALRRARRRRADVTVLFLDIDHFKVLNDSMGHLVGDEVLRVVASRVQHAI
                     RPHDVVARFGGDEFTISCEGLTGAAEAEALARELRQAIEAPVQVDEREVFLTVSVGIA
                     RGAGWESPHDLVADADAAMYQAKAAGRASVITFDERMREQSRDRLHMESALRRAIDKN
                     ELHVVYQPQVDLASGQLVGVEALLRWMSDEIGAIAPVEFIPIAEETDLIMGIGEWVLH
                     QACAQAGRWRASRGWSPLLSVNASARQFSHASAFLETVTSALHASKLEPAQLRIEVTE
                     STLGDPVATGPLVAELSRLGVSLSIDDFGTGFSSLARLRSFPVDELKIDKSFVEGLET
                     HADDRAVVAAIIGMAKALHLDTVGEGVETAGQLDILRTLGCTRVQGYYLGRPIPGNDI
                     APLLASR"
BASE COUNT         1177 a         2323 c         2360 g         1183 t
ORIGIN      
        1 tgggcgagat cgagatcgcc ggcaccgagc taggcgtggc cgccggccta gccgagatcc
       61 accctgactg gtggcggcag cgggtccgac tcgactgggt gcgcgccgag atcgcgctcc
      121 tcggggaaga cccgactgca gcgcaggagg cggcgtccga ggcgatcgat gcggccgagc
      181 tggcgcacgc gccgcgccat gtcgccaagg gtctgctgtt cctcggcgtg gcccaggctc
      241 acctcggcag ccccgacacc gtcaccacgc tgcggcgtgc cgccacgctc gccgagcagc
      301 tagggacgac gccgttgatc tggcaggcgc gagcccttgt cggtgcgcta ctcgcggagg
      361 acgacccaac taccagcgca cgcagtttgc aggctgcccg atccgcggta ttggctatcg
      421 cgggcgacct acctgatgac gttcgggagg aatggcttaa tcgtcccgat atagaggcat
      481 tgttgtcgat atagccgccc ggtttgtgca acaattgcgc cattcggagt aagggaatgg
      541 ccaggttcgg gctcaggctt gccgcccacc ctgccgatga atgagccgat ggcctaagag
      601 ggtgaaattc cccgcgggac ggaacggatt cgggaacggg cggaacactt gtgacgactg
      661 tgctcatctg tgacgatcat cggcgctttc gcgagcactt gcgccggtac gttgccgcgg
      721 tgcctggcgt cgatcgggtc atgacggcgg acaccagtgc ggagactttc gtgcggtacg
      781 ccgccgagct tccggcgctc gtcctcctcg atgtcgcgat gcccgacctt ggcggtcccg
      841 agacggcccg cctccttgtc tcggctcacc cgggcgcaaa tgtcgtcatg cttgccggca
      901 ccgcggacag tgaaggcctc gcccgcgcga tggccggcgg tgcacgcggc tacctcacca
      961 aggacgtctc cgtcgaggaa ctctgcgcgg cgctcgcgca cgtcgtcgtg gatgccggcg
     1021 tcgcccgcgc caacaatggg cacggcccgg cgcagatcat cgacccgccg ttgaccgagc
     1081 gggaacgcca ggtcctcgac gggatgagcc gcggaaagac gaacaacgaa atcggtcgcg
     1141 agctgtttct ttccgaggac accgtcaaga ctcatgcgcg gcggttgttt cgcaagctcg
     1201 gtgtcaacga ccgcgcgcag gctgtcgcct tagggtttcg cagcgggatc gtccgctagc
     1261 agtaggcccg cgcgaccgaa ggtcgcctag cggaggtcga ttgttggcgg cccgtcgtcg
     1321 gcgtcccgtc cgtcgtctcg ctccgattcg gacagcacgc cgtcgacgaa gccactggcg
     1381 tagtcccagg ccacgtagtc agcgggatcc ggttcgaacg cgggctcgtg tgcgcgcagc
     1441 tggcccacgt cgagcagctg gcgcagattt tggcggagca actcccacgc gaagtaatgc
     1501 ggcgcttcac attccccgca gtcgaccacg aggccgcgta tacctcgcgg ctcgagcagc
     1561 gtctgaaaga cctcgaggtc ggccagatcg accaggatct cctcgcggtc ctggggcgac
     1621 agcggctcgc tcgttgcctc gtgcatgcgc gaaagcctag ccgcgcagcg gccggagggc
     1681 gtgccggcga gcggacggcg acggcttagc ggcaccggca ccagccgctg tcgagagcgt
     1741 cgccagctag catggtgtct cctgcccgag gggaaagcag cccagccgtg acagattcag
     1801 cgcatgagtt cggctccttg ccggacaagg tggccatgat tggcctgacg tacgacgacg
     1861 tgctgctgct acccgcggcc tccgacgtga ttcccagcca ggtcgacacc acgacggcca
     1921 ttacccgcaa catctcgctg cgggtgccgt tgttgtcgag cgcgatggac accgtgaccg
     1981 aggcccgcat ggcgatcgcc atggcgaggc aaggtggcgt cggcgtcctg caccgcaacc
     2041 tgtcgatcga cgagcaggcg gcgcaggctg atctggtgaa acggtccgag gccggcatga
     2101 ttaccaaccc ggtgacctgc tcggccgacg acacactcgc cgatgtcgac cgcctctgct
     2161 cgcactatcg aatctccggt gctcccgtcg tcgatgctgc cggcgtgctc gttggcatcg
     2221 tcaccaaccg cgacatcaga ttcgagaccg atctgtcgcg gccggtgcgc gacgtcatga
     2281 cggcgatgcc gctcgtaacc gcgccggtcg gtgtgtccga cgacgatgcc cttgatctgt
     2341 tgcgtcgcca caagatcgag aagctgccgc tcgtcgatgg agtcgggcgc ttgcgcgggt
     2401 tgatcacggt caaggacttc accaagcgcg agcaattccc tgatgcctcc aaggacgcgg
     2461 ccggccggct gcttgtggcc gcggctgtcg gcaccggtga agactcctac aagagggccc
     2521 gcgcgctggt cgaggcaggc gtcgacatca tcgtcgttga caccgcgcac gggcattcgt
     2581 cgggtgtatt ggagatggtc agccggctca agcgcgacgt cagtgttgat gtgatcggtg
     2641 gcaacatcgc cacccgcgaa ggcgctcagg cgttggtcga cgccggcgcc gacggtgtca
     2701 aggtcggcgt cggtcctggc tcgatctgca cgacgcgggt ggtcaccgga gtcggcgtcc
     2761 cgcaggtgac ggcgatctac gaggcggcag aggcttgccg cgccgcaggc atcccggtca
     2821 tcggcgatgg cggcctgcag tactccggcg acatcgccaa ggcgatcgcg gtcggcgccg
     2881 atgccgtcat gctcggcagc ctgctggctg gcgtcgaaga gagtcctggc gagctcatct
     2941 tcatcaatgg caagcagtac aagagctatc ggggcatggg ctcgctcgcg gcgatgcagt
     3001 cacgcggcga agttcgttcc tacagcaagg acaggtactt ccaggacgac gtgttgtccg
     3061 acgacaagct ggtccccgaa ggcatcgaag gccaagtgcc gtaccgcgga ccactcgccg
     3121 ccgtcgtcca ccagctggtc ggcggcctgc ggtcagcgat gggctactgc ggcgcggcca
     3181 cgatcgacca gctgcagcag gcgcgtctgg tgcggatcac cgcggccggc ttgaaggaaa
     3241 gccatcccca cgacgtccag atgacgacgg aagcaccgaa ctactactcg cgctgatttc
     3301 tctttcatag aagggcttcc ccggtggctg aagtccagat cggcatgggc aagacgggcc
     3361 gtcgtgccta cggcttcgac gatgtcgcga tcgtgccgtc gcgacgcact cgcgaccctg
     3421 aagagatctc cgttgcctgg cagatcgatg cgtaccggtt cgaggcgccg attgtgtcga
     3481 gcgccatgga cagcgtcgtg tcgccggaca ccgcgatcga gatcggccgg ctcggtggcc
     3541 tcggcgtgct cgacctcgaa ggtctctgga cgcggtatga cgatccgcag ccgctgctcg
     3601 acgagatcgc gacaatgccc gaggccgacg tcgttcgacg gatgcgcgag atctacgccg
     3661 agccgatgaa agaggaactg atcggcgcgc gcatcaaaca gattcgcgat ggcggcgtcg
     3721 tcactgctgc cgcgttgtcg ccacagagaa cggcgcagta ccacaaggcg gtgctcgagg
     3781 ccggcgtcga tctgttcgtg atccgaggca ccacggtgtc ggccgagcac gtctcgtcac
     3841 gcgccgagcc gttgaacctc aagcggttca tctacgacct cgacgtgccc gtgatcgtcg
     3901 gcgggtccta cacctatcaa gccgcgttgc acctgatgcg taccggcgcg gccggcgtgc
     3961 tcgtcggggt cggtggcggc gccgcgcaca ccacccggca ggtgctgggc atcggcgtcc
     4021 cgatggcgac ggttgtctct gacgcagctg ccgcgcggcg cgactacatg gacgagtccg
     4081 gtggccgcta cgtccatgtc atcgcggacg gcgcgatgcg caccgggggt gacattgcga
     4141 aagcgattgc ctgcggtgcg gacgcggtga tgattggact cccgctcgcg cgcgccgccg
     4201 aggccccagg aaagggctgg cactggggcg ccgaagcccg gcacaacgac cttgcccgcg
     4261 gccagcggtt ccatgcgggc acggccggca gtctgcgcga ggtcctctat ggcccgtcga
     4321 ccgacgccga gggcactacg aatctcgtcg gggcgctgcg caaggcgatg gcgacctgcg
     4381 gctactccga cctcaaggag ttccaacgcg tcgaggtcgt cgtcacctgc tagcagtagg
     4441 gaccgaggat ggaccgctcc gcggggccga gccgctcgac gtcccatgtc aactggtgcc
     4501 agtagggata tggcaccacc ggccggctga cccgttcgat gcgcccgatg tcgtcatcgc
     4561 gcagcttcag ttcggcgctt cccaggttgt cgagcaactg ctcgggcctg cgcgctccga
     4621 cgattgccga cgtcacaccc gggcggctaa gggtgtacgc cagcgcgact tgcgccggtg
     4681 tcgcgccgcg ctcttctgcc accgagatga tctcgtcgat gatgtcgtac aaccggactt
     4741 cgtcagggac tggcggttcg gagaaggcgc tggcgaacct gcctccggcg ggaggtggct
     4801 gatttcgccg gtacttcccg gacaggagac cgccggcgag cgggctccac accagtgtcc
     4861 cgagtccttg gtcgatcgcc agggggagca gctcgaactc ggcgtcacga acgtgcggcg
     4921 tgtagtggat ctgctgggcg atcggtcgtg gcgcacccat cacatcggcc gcggcgagga
     4981 gtttcatcac ctgccacgcc gagaagttcg agacggcgac gtaacggatc ttgccagacc
     5041 ggacgagaaa ggccagcgcg tcgagggtct cctcgacgcg ggtgagccca tcccacagat
     5101 gcaggtggta caggtcgatg tggtcacgct ggagccttcg aaggctgttg tccgcggagg
     5161 ccagcacgtg gcgacgactc agccctcgat cgttaggcgt tggtcccatc ggaaaagcga
     5221 ccttgcttga gaccaagacc tccggcaggc cgttgaggag ctcgcctacg acctcttcag
     5281 ccacgccgtt gccgtatgag tcggctgtgt ccaccatgtt gacgccggca tcgatcgcca
     5341 gttccagcag gcgtcgcgcg tcgtcgaggt cgactgtcgc cgccgccacg ccggggccgg
     5401 gtgtgaacgc caccgatccc aacgtgaggc tagacacgaa taggccactg tcgccgagct
     5461 gtctgtactc catagcctca tcttgctgcg gtactcacct gctggcgagg agcggcgcga
     5521 tgtcgttgcc aggaatcggt cggccgaggt aatacccctg caccctggtg cagccaagcg
     5581 tgcgcaggat gtcgagctgg ccagcggtct cgacgccctc gcccacggtg tcgaggtgta
     5641 gggctttcgc catgccgatg atggcggcca cgacagcgcg atcgtcggcg tgcgtctcca
     5701 ggccctcgac gaacgacttg tcgatcttca gctcgtcgac cggaaaactg cgcaaccgcg
     5761 cgagtgacga gaaccccgtt ccgaagtcgt cgatcgacag cgagacaccg agccgggaga
     5821 gttccgcaac gagtggaccc gtcgccaccg gatcgccgag ggtcgactcg gtcacttcga
     5881 tgcgcagctg cgcgggctcg agcttcgatg cgtgcaaggc gctcgtcacg gtctcgagga
     5941 acgctgacgc gtggctgaac tgacgggcgg atgcgttgac ggagagcagc ggcgaccacc
     6001 cacggctggc ccgccatcga ccggcctgcg cgcatgcctg gtgcaacacc cactcaccga
     6061 tgcccatgat gaggtcggtc tcctcggcta tcggaatgaa ttcgacaggc gcgatggcgc
     6121 cgatctcgtc cgacatccaa cgcagcagcg cttcgacacc taccagctga cccgacgcga
     6181 ggtcgacttg aggctgatac acgacgtgca gctcgttctt gtcgatcgcg cgccgcaacg
     6241 ccgattccat gtgcaggcga tcgcgtgatt gttcgcgcat gcgttcgtcg aacgtgatga
     6301 cgctggcccg accggccgcc ttcgcctgat acatcgccgc gtccgcgtcc gcgacgaggt
     6361 cgtgcggcga ctcccagccg gcgccgcgcg cgatgccgac gctgaccgtc aagaacacct
     6421 cgcgctcatc gacctgaacg ggcgcctcga tggcctgccg cagttccctg gccagcgctt
     6481 cggcctcggc tgcgccggtc agtccctcac aggaaatggt gaattcgtcg ccgccgaacc
     6541 tggcgaccac atcgtgcggg cgaattgcgt gctgcacacg gctcgccaca acgcgcagta
     6601 cctcgtcgcc gaccaggtgt cccatcgaat cgttgagaac cttgaagtgg tcgatgtcga
     6661 gaaacaagac ggtcacatcg gctcggcggc gacgcgctcg gcgcagagcg gcgccgagct
     6721 cgtcgatgaa cagtcgacga tgggccaatc cggtcagcgg atcgcgaagg ttggcccgcc
     6781 gcagctctcc ctcgtcgcgg tgccgcgcta gtgcaatcgt cgccaacgcc gtggtgcgcg
     6841 cgacgtcacg tcgctcgtcg tcactcggcc cgcgggtggc ttcgaagtac gtggcaaacg
     6901 tgccaagaac ctggccgtca tggccgtcgt gatcgcgggc gatgacgggc accgaccagc
     6961 acgcaagtag tccgtgctca agagccaacg cacgccactc cgcccaatcg gggtcggtcc
     7021 cgatgtcttc tgtcaccacg tcg
//
LOCUS       DATCBJ010000391         7556 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_217378, whole genome shotgun sequence.
ACCESSION   DATCBJ010000391 DATCBJ010000000
VERSION     DATCBJ010000391.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 7556)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7556)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7556
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_217378"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..895
                     /locus_tag="VII50_07810"
     CDS             <1..895
                     /locus_tag="VII50_07810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5050793.1"
                     /translation="LGAWLAVHAPFGLLGLLVPVLVLWMSFDEQTTKSAEARLFAELA
                     RGQEQAISHSIDVSARVVLTAAARVLGGADVELVVVEHEGPVVYTGDESGHTNRRNDA
                     AAFDAPWVLRALGAGGVLIGTDDERPFCSAVVGNADQPMAVLIAHRAVGGGSFGRRET
                     GLAAVLVGQAKSWLSVAELSASRDEALARADAADMAARSLGDIGADTAPALGMLRESA
                     TRLARLAHANAGEDAVTDIVDELHSVERAVASLLGAIALAADPSLRDLESEMDAADMA
                     PARRVEDWTTTGLVSKSEFAR"
     gene            892..2010
                     /locus_tag="VII50_07815"
     CDS             892..2010
                     /locus_tag="VII50_07815"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEY5050794.1"
                     /translation="MRLPRIKDGLPLAVWAGGLVGVGCIATLAVVTVGLNAAERLDSA
                     AVLTLAVAAFTAAVATAIGALAGRRFAHTVRQLRDEAIRRLQDPSAASIGNAEDRIAV
                     ATSVELLELARTLDALHLRVRVADDVAARHRRTAETASAGMFDLLSGLVAAEESARGQ
                     LSAELHDTVAQTLMLARSVLSGGPADERELANVSNLVAEAEDQVRAVMARTRPPALRD
                     GDLAQAVIALRDDMELRYGLQVDVEWPESPYPLPLVTAVTLYRFFQESLLNVIKHADV
                     DDARLSLSVDEQSVIAIVSDNGPGFELDKVRPERGRHVGLGLLRERVRLAGGSLDVAS
                     WPGQGTTLTLRMGRGALASVPTAGEVRAASTNRAAIAG"
     gene            complement(2050..2637)
                     /locus_tag="VII50_07820"
     CDS             complement(2050..2637)
                     /locus_tag="VII50_07820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3000 domain-containing protein"
                     /protein_id="HEY5050795.1"
                     /translation="MVARDPHSELPASFAEAVASLRPVRLRAEIRLSEAPAPQRLAPF
                     AVALTADVTGASGDEDAATGRFVVLHDPAGQEAWQGSTRVVAYVRAELEPEMASDPFL
                     ADVAWTWLTEGLGRRLPERGYRALGGTVTRVTSDSYGTMAGRPLVAHVELRVSWTPDS
                     DDLGPHLEAWADVLAAAAGLAPLPPGVAALPSRRR"
     gene            2725..3642
                     /locus_tag="VII50_07825"
     CDS             2725..3642
                     /locus_tag="VII50_07825"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEY5050796.1"
                     /translation="MTALLALLASLVWGTSDFMGGVISRRVRPLVVVAVAHVIAAVTM
                     VIIAAATHSFGVPLGYLPWAAAAGVVGLVALVAFYGALSTGTMGIVAPIAGTGAVVPV
                     IVGLASGESPSGLQIAGIAAAVGGVVLASGPELAERGGPDGGGSRPLVLALVAAVGFG
                     FVFVLIHKAATHNVVMTLLAMRATSVSVVTLIGLGYLVRHRRIVSAARRHAAQHRGEE
                     PADARYGGLRRRNLPVIALIGWTDAGANGLYAYASRHGLVSVTAVLASLYPAVTVLLA
                     RFIEGERMRRIQDIGVGLAMCGVVLLAAG"
     gene            3659..4711
                     /gene="hemE"
                     /locus_tag="VII50_07830"
     CDS             3659..4711
                     /gene="hemE"
                     /locus_tag="VII50_07830"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HEY5050797.1"
                     /translation="MSSDVLLDSIFLRACRGEPVPHPPVWFMRQAGRALPEYRAVREG
                     IPMLQACTTPELIAEITVQPVRRYGVDAAIIFSDIVVPLKAIGVDLDIVPGVGPVVAS
                     PFTTSADVELLRPLEPADVEFLDTAVRLVVAGLGPTPLIGFAGAPFTLASYLVEGGPS
                     REHLKTKAMMYSQPVLWHQLLDRLATIAVAFLRVQVEAGARAVQLFDSWAGILSPADY
                     ITYVLPHSARVLAELAELRVPRIHFGVGTGELLPLMGAAGADVVGVDWRVPLDVAAQR
                     VGADHVLQGNLDPAMVFAPWDVIAQAAADVIARGRAARGHIFNLGHGVLPTTDPDVLS
                     RLVDFVHHAAGPVLGA"
     gene            complement(4681..5724)
                     /locus_tag="VII50_07835"
     CDS             complement(4681..5724)
                     /locus_tag="VII50_07835"
                     /inference="COORDINATES: protein motif:HMM:NF025616.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4349 domain-containing protein"
                     /protein_id="HEY5050798.1"
                     /translation="MRQFKGSRMMTVLGVTAVVAVLATGCTSAVKRSSAAPAASAAAG
                     TSAAGPANNYLTEVPVSAAPAANSEVAAAPSAPAAAASSAAPAAGGAGQNVSQPLLTG
                     RQVIFTADITVQTKDIKTAVGQVTQIANGRGGLVFAEQVNLASKDPTNPGAASATVTL
                     KVPPDTLDQGILDQIGRLGTELSRNETASDVTAQVVDVNSRVAAAQDSLTRLRQLFQH
                     AGNVADLATVENEIAQREADLESLEAQQRTFTLQTAQATITLNLVATPKVAVLPAPVK
                     KAHVVGFVRGLRGGWHAFTRTVSAVTTALGALLPFLGVLLVLGLVALIVRRRLLIPPA
                     DDATHAPSTGPAA"
     gene            5834..7267
                     /gene="hemG"
                     /locus_tag="VII50_07840"
     CDS             5834..7267
                     /gene="hemG"
                     /locus_tag="VII50_07840"
                     /EC_number="1.3.3.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004729 - oxygen-dependent
                     protoporphyrinogen oxidase activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protoporphyrinogen oxidase"
                     /protein_id="HEY5050799.1"
                     /translation="MSALPSDTPGPVSAAASRPHVVVVGAGISGLAAARALVLDGRVQ
                     VTVLEGSPRTGGKLLRGRVAGVETDLGAEAVLARRPEGVALIGSLGLSPVGPTTMQAA
                     IWTRGQLRLLPANHVMGVPVSVRSLAASGVVSRAGVARASLDMTLPATALGDDVSVAA
                     YVGARMGHEVVDRLVEPLLGGVYAGRADELSLRATLPQVAAVASQRSLFRGLRSRTSQ
                     PQAGPVFAGLAGGVGQLAEAVTAAVTAAGADVVLDTTVRELARLPVGWRLVAGSTANP
                     KTINADAVVLALPATPASRLLAATAPWAARDLAGIDYASMAIITLAFSASAFGKPLTG
                     SGFLVPAVDARLIKAATFSTQKWGWYDESDGVLVRCSVGRYGDVGDLQRSDDELVAGA
                     VRDLAAAVGVRGVPRDAVVTRWGGALPQYSVGHLERVARIKAALAAVPGLAACGAAFD
                     GVGIPACIGSGTAAATQVLAKLFGEGE"
     gene            7273..>7556
                     /locus_tag="VII50_07845"
     CDS             7273..>7556
                     /locus_tag="VII50_07845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chlorite dismutase family protein"
                     /protein_id="HEY5050800.1"
                     /translation="MSDAESTSHGLQAAAAAESAAQVPLTAYTMYAVFRSQRGAGALP
                     DSGRDLAAAEVDDLLAELAGKGVVTRGFYSVSGFRHDADYLIWWIAPNSE"
BASE COUNT         1045 a         2506 c         2704 g         1301 t
ORIGIN      
        1 gctcggtgcc tggctcgccg tccatgcgcc gttcggactt ctcggtctac ttgtgccagt
       61 cctggtgttg tggatgtcgt tcgacgaaca gacgacgaag tcggccgaag ctcggctgtt
      121 cgcggaactc gctcgcggcc aggagcaggc gatctcccat tcgatcgatg tatccgcccg
      181 cgtcgttctc acggctgccg cgcgtgttct cggcggcgct gacgtcgagc tggtcgtcgt
      241 cgagcacgag ggcccggtcg tctacaccgg cgacgagagc ggccacacca accggcgcaa
      301 tgacgcggcc gcattcgatg caccgtgggt attgcgtgcc cttggcgcgg gcggcgtact
      361 catcggaacc gacgacgaac gcccattctg ttcggcagtc gtgggcaacg ctgaccagcc
      421 aatggctgtc ctgatcgccc atcgcgcagt cggcggcggc agcttcggcc gtcgcgagac
      481 cgggcttgcc gcggtcctgg tcgggcaggc gaagtcgtgg ctctcggtcg ccgaactctc
      541 cgcatcccgc gacgaagcgc tcgctcgcgc ggacgccgcc gatatggctg cccgttcatt
      601 gggcgacatt ggcgctgaca cggcccctgc tcttggcatg ctccgcgaat cagcgacccg
      661 gctcgcccgg ctggcccacg cgaatgccgg cgaggacgca gtcaccgaca tcgtcgacga
      721 gctgcactcg gtcgagcgcg ccgttgcgtc attgcttggt gcgatcgccc tcgcggccga
      781 cccgtcgctt cgcgatctcg agtccgagat ggacgccgcg gacatggcgc cagcccgccg
      841 cgtcgaggac tggaccacga ccggactcgt ttcgaagtcg gagttcgcac gatgagactg
      901 ccgagaatca aggacggcct tccgctcgcg gtatgggcag ggggcctcgt cggcgttggc
      961 tgcatcgcca cgctcgcggt cgtcaccgtc ggcttgaacg cggcggagcg gctggacagt
     1021 gccgcggtac tgacgctcgc cgttgcggcc ttcacggccg ctgtggcgac cgcaatcggc
     1081 gcgctggcgg gccgccgttt cgcgcacacc gttcgacagt tgcgagacga ggcgatccgc
     1141 cggctgcagg acccctccgc ggcgtcgatc ggcaatgccg aagaccggat cgcggtagcg
     1201 acaagcgtcg agctgctcga actggcgcga actcttgacg ccctgcacct gcgggtgcgc
     1261 gtggccgatg acgtagcagc gcggcaccgg cgcaccgccg agaccgcaag tgcgggcatg
     1321 ttcgatctgc tctccggcct ggtcgccgcc gaggaatcgg cacgcggaca gctgtcagcc
     1381 gagctgcacg acacggtggc tcagacattg atgcttgccc gcagcgtgct atcaggaggg
     1441 ccggcggacg agcgcgagct cgccaacgtc agcaacttgg tagcggaggc ggaggaccag
     1501 gtgcgtgccg tcatggcccg cacccggccg ccggcgctgc gcgacggcga cctcgcacag
     1561 gccgttatcg cgctgcgcga cgacatggag ctgcgctacg gcctccaggt cgacgtcgaa
     1621 tggcccgagt cgccgtaccc gctgccgctg gtcaccgcgg tcacgctgta ccggttcttc
     1681 caggagtcgt tgctcaacgt catcaagcac gccgacgtcg acgacgcacg gctgtcgctg
     1741 tccgtcgacg agcagagtgt gatcgcgatc gtgtcggaca acggccccgg cttcgagcta
     1801 gacaaggtgc gcccagagcg cggtcgccac gtcggccttg ggttgttgcg cgagcgagtc
     1861 cgtctcgcgg gcggctcgct tgatgtcgcg tcgtggccgg ggcagggcac cacgctcacc
     1921 ctgcgcatgg ggcgcggtgc attggcgagc gttccgacgg ccggtgaagt cagggcggca
     1981 tcgaccaacc gtgccgcaat cgcgggctag ctcgacgcgc tcagttcggt ccagccctcg
     2041 cctgctcgtt cagcggcggc ggctcggcag cgctgcgacc cctgggggca acggagccag
     2101 acccgccgca gccgcgagga cgtcggccca cgcctcgaga tgtggtccga gatcgtcgct
     2161 gtccggcgtc caggacaccc gcaattcgac gtgtgcgacg agcgggcgac ccgccatcgt
     2221 cccgtaggag tccgaggtca cgcgggtgac cgtgccgccc agcgcgcggt atccgcgctc
     2281 gggtagccgc cggcccagac cttcagtgag ccaagtccag gcgacgtcgg cgaggaacgg
     2341 gtcactggcc atctccggct caagctccgc gcgcacgtag gcgacgacgc gggtcgatcc
     2401 ctgccaggct tcttggccgg cgggatcgtg cagcacgacg aaacggccgg ttgcggcgtc
     2461 ctcgtcgccg ctcgcgcctg tgacatcggc cgtgagcgcc acggcgaatg gtgcgagtcg
     2521 ctggggcgcg ggagcctccg acagccggat ctccgcgcgc agccgcacag gacgcaggct
     2581 ggcgaccgcc tccgcaaagc ttgcgggcag ttcgctatgc ggatccctgg caaccacact
     2641 ggcagcgtag gtgggattgc accggcctgg gagacggcgc gccgaccggc cgccgggtgg
     2701 gcgccctccc gtagcctcag gatcgtgacg gccctgcttg cgctcctcgc gagcctcgtg
     2761 tggggcacct ccgacttcat gggcggcgtc atcagccggc gggtgcgtcc gctggtcgtg
     2821 gtcgcggttg cccatgtgat cgcggccgtg acgatggtga tcatcgcggc cgcgacgcac
     2881 agtttcggcg tcccgcttgg atatttgccg tgggccgcgg ctgccggcgt cgtcggactc
     2941 gtcgcgctgg tggccttcta cggcgcgctg tcgacgggaa cgatggggat cgtcgcgccg
     3001 atcgcaggta ccggcgcggt ggtgccggtc atcgtcgggc tggcctccgg ggaatctcca
     3061 tcgggcctgc agatcgccgg gatcgccgcg gcggtcggag gcgtagtcct cgccagcggg
     3121 ccggagctgg ccgagcgggg cgggccggac ggcggcggct ctcggccgct cgttctcgca
     3181 ctcgtcgcgg cggtgggctt cggattcgtc ttcgtgctga tccacaaagc ggccacccac
     3241 aacgtggtga tgacgctgct cgccatgcga gccaccagcg tgtcggttgt cacgctgatc
     3301 gggctcggct acctcgtgcg gcacaggcgc atcgtgtccg ccgccagacg ccacgcagcg
     3361 cagcaccgag gcgaagagcc ggccgatgca cgctacggcg ggctgcgtcg gcgcaatctt
     3421 ccggtgatcg ccttgatcgg ttggaccgac gcgggcgcga acggcctgta tgcatacgcc
     3481 agccgccatg ggttggtcag cgtgacggcc gtcctcgcgt cgctctaccc ggcggtgacc
     3541 gtattactcg cgcggttcat cgagggcgag cgcatgcggc gcatacaaga catcggcgtg
     3601 ggccttgcga tgtgcggcgt cgtgctactc gccgccggct gatgggagca tgggtccggt
     3661 gagctccgac gtgctgctcg acagcatctt cctgcgcgcc tgccgtggcg aaccggtccc
     3721 gcatccgccc gtctggttca tgcggcaggc cggccgcgcg ctgccggaat atcgcgcggt
     3781 tcgcgagggc atcccgatgc tgcaggcgtg tacaacaccg gagctgatcg cggagatcac
     3841 cgtgcagccg gtccgccgct atggcgtcga tgcggcaatc atcttcagcg acatcgtggt
     3901 cccgttgaag gcgatcggcg tcgacctcga catcgtgccc ggtgtcggtc cggtcgttgc
     3961 ctcgcctttc acaaccagcg cggacgtcga attgctgcga ccactcgaac ccgctgacgt
     4021 cgagtttctg gatacagcag tgcgattggt cgtggccggg ctcggcccga cgccgctcat
     4081 cgggttcgcg ggagcaccgt tcacgttggc tagctatctc gtcgagggag gcccgtcacg
     4141 cgagcacctg aagaccaagg cgatgatgta ttcgcagccc gtcttgtggc accagctgct
     4201 cgaccggctt gcgacgatcg cggttgcctt cctgcgcgtt caggtcgaag ccggagctcg
     4261 cgccgtccag ctgttcgatt cgtgggccgg catattgtcg ccggccgact acatcaccta
     4321 cgtgctgccg cacagtgccc gcgtactcgc ggagctcgcc gagctgcggg tgcctcgtat
     4381 tcactttggc gtcggcactg gtgaactgct cccgctgatg ggtgcggccg gagccgacgt
     4441 cgtcggtgtc gactggcggg tgccgctcga cgtcgccgcg cagcgcgtcg gcgccgacca
     4501 tgtgctgcag ggaaatctcg atcctgcaat ggtttttgcg ccgtgggatg tcatcgcgca
     4561 ggcggcggcc gatgtcatcg cgcgcggccg ggccgcgcgc ggtcacatct tcaacctcgg
     4621 acacggtgtc ttaccgacga ccgatcccga cgtgttgtcg cggctagtcg acttcgttca
     4681 tcacgctgcc ggacctgtgc tgggcgcgtg agtcgcatcg tctgccggtg gtatcagcag
     4741 ccggcgccgc acgatcagcg ctacaagacc taacacaagc aggacgccga ggaacggcag
     4801 cagtgcgccc agtgcagtgg tgacggcact cacggtgcgg gtaaatgcgt gccagccgcc
     4861 acgcaagcca cgcacgaacc cgacgacgtg cgccttcttg accggtgccg gaagcacggc
     4921 gaccttcggc gtggcgacca ggttcagcgt gatggtggcc tgcgcagtct gcaaggtgaa
     4981 cgtgcgctgc tgcgcctcga gcgactcgag gtcagcctcg cgttgggcga tttcgttctc
     5041 gacggtggcg aggtctgcga cgttaccggc gtgctggaac agctggcgaa gtctcgtcag
     5101 gctgtcctgc gcagctgcga cgcgcgagtt gacgtcgaca acctgggcgg tgacgtcgct
     5161 agccgtttcg ttgcgactga gctcggtgcc gagccggccg atctgatcga ggatgccctg
     5221 gtcgagggtg tccggcggca ccttcaaagt gacggtcgcg cttgcggcgc ctggattggt
     5281 ggggtccttc gaggccaggt tgacctgctc ggcgaagaca aggccgccac gaccgttggc
     5341 gatctgggtg acctggccga ccgcagtctt gatgtccttc gtctgcaccg tgatgtcggc
     5401 ggtaaagatg acctgccggc cggtgagcag cggttgcgac acgttctggc ctgcaccccc
     5461 cgcggctggt gcggcactcg aggcggccgc ggctggcgcc gacggagctg ccgcgacttc
     5521 gctgttagcc gctggtgccg ccgacacagg tacctcggtc aggtagttgt tcgcaggccc
     5581 cgcggcgctg gtgcctgccg cggcggaagc cgctggagcc gcgctcgacc gcttcaccgc
     5641 agatgtacag ccggtagcga gtacggcgac caccgctgtg acgccgagta cggtcatcat
     5701 tcgcgaaccc ttgaactggc gcatggcgtc ctcccgatgg ccggtgagtt ccggtaccga
     5761 tgagatcgcc gccgcgaccg cgcggttccc gcccgttgat cacgatcggg ccacggtctg
     5821 cgatggtgaa cccgtgagcg cgctcccgtc agacacccca ggtccggtca gtgccgcggc
     5881 ctcgcggccg cacgtcgtcg tggtgggggc ggggatttcg ggcctagcag cggcccgcgc
     5941 gctcgtgctc gacggtcggg tccaggtcac cgtgctcgaa ggatcgccgc gcaccggcgg
     6001 caagttgctg cgtggccgcg ttgccggcgt cgagacggat ctcggtgctg aggcggtgct
     6061 cgcccgtcgt cccgagggcg tggcgctcat agggtcgctc gggctcagcc cggtcgggcc
     6121 gaccacgatg caggcggcga tctggactcg cgggcagctg cggctcttgc ccgccaatca
     6181 cgtgatgggc gtgccggtca gcgtccgctc gctcgcggcg tccggcgttg tttcgcgggc
     6241 cggcgtcgcg cgggcttcgc tcgacatgac gttgccggcc acggcgctcg gagacgatgt
     6301 ctcggttgcc gcctatgtcg gcgcccggat ggggcatgag gtggtcgacc ggctggtcga
     6361 gccgctgttg ggcggcgtct acgcggggag ggcggatgag ttgtctcttc gagcgacgtt
     6421 gccgcaggtt gccgcggtgg cctcgcagcg ttcgttgttc cgcgggctgc gctcgcgcac
     6481 atcgcaacct caggcgggac cggtgtttgc cgggcttgcg ggcggagtcg ggcagcttgc
     6541 cgaagccgtg acggccgcag tgacggcggc cggcgccgac gtggtgctcg atacgacagt
     6601 gcgtgaactt gcgcggcttc cggtcgggtg gaggttggtg gcaggttcga ccgccaaccc
     6661 gaagaccatc aacgcggacg ccgtcgtgct ggcgttaccc gcaacgccgg ccagtcgcct
     6721 cttggccgcg acggcaccat gggcggcgcg ggatctggcc gggatcgact acgcgagcat
     6781 ggcgatcatc acgcttgcat tctcggcctc ggccttcgga aaacccttaa ccggcagtgg
     6841 ttttctggtc ccagcggttg acgccaggct gatcaaagcc gcgacattct caacacagaa
     6901 gtggggctgg tacgacgagt cggacggcgt tttggtgcgc tgttccgtcg ggcgctacgg
     6961 cgacgtcggc gacctccagc gatcagacga cgagctcgtc gcaggcgcgg tgcgcgatct
     7021 cgcggcggcg gtcggcgtcc ggggcgtacc gcgcgatgcg gtcgtcacgc gctggggtgg
     7081 tgcgctgccg cagtacagcg tcggccatct cgagcgggtg gctcgcatca aggcggcact
     7141 ggccgctgtg ccgggcttgg cggcctgcgg agccgcattc gacggggtcg ggattcccgc
     7201 atgtatcggc agtggcacgg cggcggcgac ccaggtcctc gcaaagctct tcggcgaggg
     7261 agaataggtg ttatgagcga cgcggagtca acaagtcatg ggttgcaggc ggctgctgct
     7321 gcggagtcgg cggcgcaggt accactgacg gcgtacacga tgtacgcggt attccgatcg
     7381 cagcgcggcg cgggggcgct acccgattcg ggccgcgatc tggccgctgc cgaggtcgat
     7441 gacctgctgg ccgagttggc cgggaaaggt gtcgtcaccc gcgggttcta cagcgtcagt
     7501 ggcttccgac acgacgccga ctacctcatc tggtggatcg cgccgaactc cgagga
//
LOCUS       DATCBJ010000392         6731 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_239928, whole genome shotgun sequence.
ACCESSION   DATCBJ010000392 DATCBJ010000000
VERSION     DATCBJ010000392.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 6731)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6731)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6731
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_239928"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..872
                     /locus_tag="VII50_07850"
     CDS             <1..872
                     /locus_tag="VII50_07850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HEY5050801.1"
                     /translation="GAEPPAVRDSQGRVVIRPPGQVAPESVPVTDAWVEGSGGRIHAL
                     ISKPFDSSAVEDGPPHPLPTVFVVHGGPTVHDTDAFFPGVAAWVDSGFVVVRVNYRGS
                     TGYGSAWRDAIEHRVGLTELEDLVAVRDWAVQSGLADASRIVLTGGSWGGFLTLLGLG
                     LTPDAWSVGVAGVPVADYVAAYEDEMEGLRAYDRALFGGTPDEVPDRYEQSSPITYVD
                     DVKAPVFISAGANDPRCPIRQIENYVERLRERGADFSVYRFDAGHGSLVVDERIRQTE
                     AQINFARTHLGMD"
     gene            1314..1499
                     /locus_tag="VII50_07855"
     CDS             1314..1499
                     /locus_tag="VII50_07855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5050802.1"
                     /translation="MADFAQLIEAVGMRLVVVDRKGREVVPEEEQDSKLRDRGGRRFP
                     AHLDVRDGKDDWWGDGG"
     gene            complement(1624..2685)
                     /locus_tag="VII50_07860"
     CDS             complement(1624..2685)
                     /locus_tag="VII50_07860"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HEY5050803.1"
                     /translation="MSGGSSRAYRVGVVGATGQVGAMMRRVLAERGFPLSELRFFASA
                     RSAGTKLPWAGVDITVEDAELADPSGLDIALFSAGAATSRLLAPKFAAAGVMVIDNSS
                     AWRMDPDVPLIVSEVNPLEVQSARRRIIANPNCTTMAAMPVLKPLHDEAGLVRLIVST
                     YQAVSGAGLSGVDELDKQVRQVVDRAAELTLDGSAVEFPTPVKFVRPVAFNVLPLAGS
                     IVDDGSFETDEEQKLRNESRKILGIPSLRVSGTCVRVPVFTGHSLSINAEFSRALPVA
                     RALELLADSPGVELTAIPTPLQAAGADPSFVGRVRQDPGVDGSRGLALFVSNDNLRKG
                     AALNAVQIAELVAADLASH"
     gene            complement(2682..3950)
                     /locus_tag="VII50_07865"
     CDS             complement(2682..3950)
                     /locus_tag="VII50_07865"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013230598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HEY5050804.1"
                     /translation="MGVVVQKYGGSSVADAERIKKVAQRIVATRKDGHDVCVVVSAMG
                     DDTDKLLDLAAQVSPLPPARELDMLLTAGERISMALLAMAIANLGMKARSFTGSQAGV
                     ITDSKHGRAQIIDVAPSRIREALDQGDIAIVAGFQGVSQDTKDVTTLGRGGSDTTAVA
                     LAVALGADVCEIYTDVDGVFTADPRIVPTARRIDRISYDEMLEMAAAGAKILHLRCVE
                     YARRYALPIHVRSSFSTKTGTWIVEIPAEEAMEAPIISGVAHERGEAKITVVGVPDKP
                     GEAAQIFTVVADAGVNIDMIVQNVSSVATGLTDITFTLPSTDLAAAVAALSRAQAQIG
                     FESLQSDDQIGKVSLVGAGMRTHPGVTARFLTALADAGINVEMISTSQIRISVVVRED
                     ELPIAVAAVHSAFELDSPDDVATVYGGTGR"
     gene            4016..4813
                     /locus_tag="VII50_07870"
     CDS             4016..4813
                     /locus_tag="VII50_07870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5050805.1"
                     /translation="MDFVRAEAAGKRGRSPRRADVGVLAAVLGTVFALSAGCTSHSHA
                     PAPPASLLASSSGPSAATQLSAIAAIARAAQYTGTYSADSSDNPPRASTIVAYRTPTS
                     TRLDITEAVGRVVIQVDPTGTYSCNLPTGGTPSCLTLAGPGQPVPANVNPSGQALFTT
                     TLDTLAQGVDLTVSVEAPRPAANGIPASSCFALIAAPDQVSPGTYCFSATGVLTRAQF
                     RSNVLELTALTTAPVQSDFLLPASPSPVSSAAASGAPASSASPAISS"
     gene            complement(4810..5121)
                     /locus_tag="VII50_07875"
     CDS             complement(4810..5121)
                     /locus_tag="VII50_07875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5050806.1"
                     /translation="MLLPTSCTITGGVATATGTFNPQMCGEGYRRAGDVVELYVYSAP
                     SVGDPSQLGKLSAEHPVAVTNTGPWVVAVSIDTSFGQRPAQCLVAVQSTHAEMGAPND
                     Y"
     gene            complement(5132..5362)
                     /locus_tag="VII50_07880"
     CDS             complement(5132..5362)
                     /locus_tag="VII50_07880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5050807.1"
                     /translation="MSPSNKRSLAIVGSVLLIGHCVAAFFIGKHAGSRGCAACGADHV
                     GYAELTAALAIGPSIQFPHDSGAHLGAAVVRP"
     gene            complement(5372..5923)
                     /locus_tag="VII50_07885"
     CDS             complement(5372..5923)
                     /locus_tag="VII50_07885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5063 domain-containing protein"
                     /protein_id="HEY5050808.1"
                     /translation="MSELVVEGPSDDLTDFAAEIADQVESFVIAVREVARGAEPGGAV
                     SMLLLEVSQLLLAGGRLGAIGDVVPTERWEPDAGPDPDIDELRERLAALLAPIDRYVE
                     VFDPYEAPPSAANFVLSDDLADVTGDLLHGLQHYQAGRPIEALWWWQFSYVSHWGTHA
                     GAALRALQSVVAHARLDVEGLAG"
     gene            complement(5916..6515)
                     /gene="recR"
                     /locus_tag="VII50_07890"
     CDS             complement(5916..6515)
                     /gene="recR"
                     /locus_tag="VII50_07890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020124031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HEY5050809.1"
                     /translation="MYEGVVQDLIDELGKLPNIGPKSAQRIAFYLLAADAVDVKRLAQ
                     VLLEVKDKVRFCEICGNVAEEEQCRICRDPRRDLSVICVVEESKDVVAVEKTREFRGR
                     YHVLGGAISPIEGIGPDDLRVRELMTRLADGTVTELILATDPNLEGEATATYLARLVK
                     PMGLRVTRLASGLPVGGDLEYADEVTLGRAFEGRRQIDV"
     gene            complement(6519..>6731)
                     /locus_tag="VII50_07895"
     CDS             complement(6519..>6731)
                     /locus_tag="VII50_07895"
                     /inference="COORDINATES: protein motif:HMM:NF014616.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HEY5050810.1"
                     /translation="KAVDPADTEALADLVLAAVRAATGRAAALQAAKLGPLAQGFGGL
                     GQAGGLGDLDDGGLGPLPGGLPGLGL"
BASE COUNT         1147 a         2300 c         2203 g         1081 t
ORIGIN      
        1 cgggtgccga gccgccggcg gtgcgcgatt cgcaaggacg cgtcgtcatc cggccgcccg
       61 ggcaggtcgc acccgaatcc gtccctgtca cggatgcctg ggttgaaggc tcaggcggcc
      121 gcattcatgc actgatctcc aagccgttcg actcgtccgc ggtcgaggac ggacccccac
      181 atccgttgcc gacggtgttc gtcgtacacg gagggccgac cgtgcacgac actgatgcgt
      241 tcttccctgg ggtcgcggcg tgggtcgatt cgggcttcgt cgtcgtccgc gtgaactacc
      301 ggggttcgac gggctacggc tcggcgtggc gcgacgcgat tgagcaccgg gtcggactca
      361 ccgaacttga ggatctggtc gccgtgcggg attgggcagt gcagtcgggc ctcgcggatg
      421 cgtcccgcat cgtcctcacc ggcggctcgt ggggcggctt cctcacgctg ctcggccttg
      481 ggctgacccc cgacgcgtgg tcggtcggtg tggcgggagt gccggtggcc gactacgtcg
      541 ccgcctacga agacgagatg gaaggcctgc gcgcgtacga ccgcgcactg ttcggcggta
      601 cccctgacga ggtgcctgat cgctatgagc agtcgtcacc gatcacctac gtcgacgacg
      661 tgaaagcacc tgttttcatc tcggccggtg cgaatgaccc gcgctgcccc atccggcaga
      721 tcgaaaacta cgtggaacgc ctacgcgaac gcggagccga cttctcggtc taccgcttcg
      781 acgccggcca cggctcgcta gtggtcgacg agcgcatccg ccaaaccgag gcccaaatca
      841 acttcgcccg cacccatctc ggcatggact gaccagcccg cccgacactg gtcagttgcg
      901 cttcgcccgc cgcagacgga tgtcctcgcg gttcctttgc tctgcagtgc tatgactgca
      961 gaacaggtct ggagcgatct gactgcagag caggtctgga gcgatctgac tgcagagcag
     1021 gtctgcagtc ctatgactgc agcacacgtc tgcagcgatg gcgctgccga ccaaagcgcg
     1081 gagaagaggc ggccgcggag cgccctgtcc acaaccgagg cgccgttctc atacgtcggc
     1141 tttgtgctcg atcgacgggc cgcgtccgcc gccagactcc tgtccatgcc ggggtttccc
     1201 gtagatcgat acatgcgcgg tgtaagaagg ctcgtcgatc tgagtcagcg tgagttcgca
     1261 ttgcgcgccg gcgtggcgac tttctcgtcg gaaaggaacc acgccccgcg cgggtggctg
     1321 atttcgcgca gctgatcgaa gccgtgggca tgcgcttagt ggtcgtggac cgcaaaggac
     1381 gcgaggtcgt gcccgaagag gagcaggact cgaagctcag ggatcgcggc gggcggcgat
     1441 ttcccgcgca tcttgatgtg cgcgacggca aggacgactg gtggggtgac ggggggtgac
     1501 gggtggccga tgtccaaggg aagacgccgg aatacacgtt cgaccgcagc cgtgggtacc
     1561 gagaccggcg acgcgaggcc caacgcccgt gaggtcgagc gcaccgagca ggcacgccgc
     1621 gagctagtgc gaggcgaggt ctgcggcgac gagttcggcg atctggaccg cgttcagcgc
     1681 agcgcccttg cggaggttgt cgttgctgac gaacagggca agcccgcgag aaccgtcgac
     1741 tccggggtct tgccgtaccc ggccgacgaa actggggtcg gcgccggccg cctgcaacgg
     1801 agtggggatc gcggtcagct caacgcccgg ggagtcggcg agcaactcca gagcccgcgc
     1861 gacgggcaga gcccgtgaga actcggcgtt gatcgacagc gagtgcccag tgaacacagg
     1921 aacccgaacg cacgtgcctg acaccctcaa cgacggaatc ccgagaatct tgcggctctc
     1981 gttgcgcagc ttctgctcct cgtctgtctc gaacgacccg tcgtcgacga tcgatccggc
     2041 gagtggcagc acgttgaacg cgaccggccg cacgaacttc accggcgttg ggaactcgac
     2101 cgccgatcca tcgagcgtga gctcggccgc gcggtcgacg acctggcgca cttgcttgtc
     2161 gagttcatcg accccggaca gtccggcgcc ggacactgct tgataggtgc tgacgatcag
     2221 gcgaacaagc cccgcttcgt cgtgcagcgg cttcaacacc ggcatcgctg ccatcgtcgt
     2281 gcagttcggg ttggcaataa ttctccggcg cgccgactgc acctctagcg ggttgacctc
     2341 gctcacaatg agcggcacat ctgggtccat ccgccacgcg gaggagttgt cgatcaccat
     2401 gacgcccgcc gcggcgaact tcggtgcgag caagcgcgac gtcgctgcgc cggcggagaa
     2461 cagcgcgatg tcgagcccag acgggtcagc caattcggcg tcctcgacgg tgatgtcgac
     2521 cccggcccac ggcaacttcg taccggccga gcgggctgac gcgaagaacc gaagctccga
     2581 gaggggaaat ccgcgctcag cgagcacccg gcgcatcatc gcgccgacct gaccggtggc
     2641 cccgaccaca cccacgcggt acgcgcggga cgagccgcca ctcatcgccc ggtcccaccg
     2701 tagacggttg ccacgtcgtc gggagagtcc aactcgaatg cgctgtgcac ggcggcgaca
     2761 gcgattggca gttcgtcctc acggaccaca acagagatgc gaatctgcga cgttgagatc
     2821 atctcgacgt tgatcccggc gtccgccaag gctgtgagga agcgcgcagt cacaccggga
     2881 tgcgtccgca tcccagcgcc aaccagtgac accttgccga tttggtcgtc ggactgcagc
     2941 gactcgaacc cgatctgtgc ctgcgcgcgc gacaaagcgg cgaccgctgc ggcgagatcg
     3001 gtgctgggca aggtaaatgt gatgtccgta agtccggtcg ccacggacga cacgttctgc
     3061 acgatcatgt cgatgttcac gccggcgtcg gcgaccacgg tgaagatctg cgcggcctca
     3121 ccgggcttgt ccggcacccc gaccacggtg atcttcgctt cgccccgctc gtgcgcgacg
     3181 ccggagatga tcggggcttc catggcttcc tccgcaggga tctcgacaat ccacgtcccg
     3241 gtcttcgtcg agaacgacga tcggacgtgg atcggcagtg cgtaacgtcg tgcgtactcc
     3301 acacatcgca agtgcaggat ctttgcgccg gccgcggcca tctcgagcat ttcgtcatac
     3361 gagatccggt cgatccggcg cgctgtcggc acgatgcgcg gatccgcggt gaacaccccg
     3421 tcgacgtccg tgtagatctc gcaaacgtcg gcacccaggg cgaccgccaa cgccaccgcc
     3481 gtggtgtccg acccaccgcg acccaacgtt gtgacgtcct tggtgtcttg cgacacgccc
     3541 tggaagccgg ccacgatcgc gatgtcgccc tgatcgagcg cctcgcgaat tcgactggga
     3601 gcgacgtcga tgatctgcgc cctgccgtgc ttggagtcgg tgatcacacc ggcctggctg
     3661 ccggtgaacg aacgggcctt catgccgagg ttcgcgatgg ccatggccag cagtgccatc
     3721 gagatgcgct cacctgccgt gagcagcatg tccagctcgc gggccggcgg cagcggcgag
     3781 acctgcgcgg cgaggtcgag aagcttgtcg gtgtcgtcgc ccatcgcgga caccaccacg
     3841 cacacgtcgt gaccgtcttt gcgggtcgcc acgatccgct gcgcgacctt cttgatgcgc
     3901 tcagcgtcgg cgacggacga gccaccgtat ttttggacga cgacgcccac gactaaacaa
     3961 ctccttggca gggatgaccg cgggcaagtc taccggggcc cgttagcctg accttgtgga
     4021 tttcgtccgg gccgaggcgg caggcaagcg cggcagatcg ccccggcggg ccgacgtcgg
     4081 ggttctcgcg gccgttctgg ggaccgtctt cgcgctcagc gcagggtgta ccagccactc
     4141 gcacgccccc gcgcccccgg cgtccctgct cgcttcgtcc tccggaccga gtgccgcaac
     4201 ccagctgtca gcgatcgccg cgatcgcccg cgcggcgcaa tacacgggca cgtactccgc
     4261 cgacagctcc gacaacccgc ctcgcgccag cacgatcgtc gcgtaccgca ccccgaccag
     4321 cacccggctc gatatcaccg aagctgtggg tcgcgtggtg atccaggtcg acccgaccgg
     4381 gacctatagc tgcaacctgc caacaggcgg gacgccgagt tgcttgacgt tggccggacc
     4441 aggccagccg gtgccggcga acgtgaatcc atccgggcaa gcgctgttca ccacgaccct
     4501 cgacacgctg gcgcaaggtg tcgacctcac cgtctccgtt gaagccccgc ggccggcggc
     4561 gaacgggatc cctgcgtcgt cctgcttcgc gttgatcgcc gcgcccgacc aggtgtcgcc
     4621 tgggacgtac tgcttcagtg ccaccggcgt tctcacccgc gctcagttcc gctcgaacgt
     4681 gctcgaactg actgcactga cgaccgcacc ggtgcagagc gacttcttgc tccccgcgtc
     4741 cccgtctccg gtcagttcag ccgctgcgag tggtgcccct gcaagcagcg cgagtcccgc
     4801 gatcagcagt tagtagtcgt tcggggcgcc catctccgcg tgggtcgact gcacagcgac
     4861 gaggcattgc gcgggccgct gtccaaacga ggtgtcgatc gaaacggcaa cgacccaagg
     4921 gccggtgttc gtgacggcga ccggatgttc cgcggacagt ttgcccagct gactggggtc
     4981 gcccacgctc ggagccgagt acacgtacag ctcgaccaca tcacccgccc gccgataccc
     5041 ctcgccgcac atctgcgggt tgaacgtgcc ggtcgcggtc gcgactccgc cagtgatcgt
     5101 gcacgaagtc ggaagcaaca cggcagggct ctcatgggcg gacgacagcg gcaccgaggt
     5161 gtgcaccgga atcgtggggg aactggatgc tcggaccgat ggcgagggcg gcggtgagct
     5221 cggcgtagcc gacgtggtcg gcgccacagg cagcgcagcc ccgactgccg gcgtgcttgc
     5281 caatgaagaa cgcggcgacg caatgcccaa tcaggagtac agatccgacg atggcaaggc
     5341 tgcgcttgtt tgacgggctc atcaagccga cctagccagc cagcccctcg acgtccagcc
     5401 ttgcgtgggc aacgacggat tgaagggcgc gcagagcggc cccggcgtgg gtgccccagt
     5461 gcgagacata ggagaactgc caccaccaca gcgcctcgat gggccggccg gcctggtagt
     5521 gctggaggcc gtgcagcagg tcgccggtga cgtcggcgag gtcgtcggag agcacgaagt
     5581 tggcggcgct cggcggcgcc tcgtaggggt cgaagacctc gacgtagcga tcgatggggg
     5641 cgagcagcgc agcaaggcgc tcacgcaact cgtcgatgtc gggatcgggc ccggcgtcgg
     5701 gctcccagcg ttcggtcggg acgacgtcgc cgatcgcgcc gagccggccg cccgccaaca
     5761 gcagctgtga cacctcgagg agcagcatcg agacggcgcc tccgggctcc gcaccgcggg
     5821 caacctcgcg caccgcgatg acgaagctct cgacctggtc cgcgatctcg gcagcgaaat
     5881 cggtgagatc gtcggatgga ccttcgacca cgagctcaga catcgatctg tctcctcccc
     5941 tcgaacgcac gaccgagcgt gacctcatcg gcatactcca ggtccccgcc gaccggcaga
     6001 ccactcgcca acctggttac ccgcaatccc atcggcttca ccagccgcgc caagtaggtg
     6061 gcggtggctt cgccttcgag attcgggtcg gtcgcgagga tcaactcggt cacggttcca
     6121 tcagcgagcc tagtcatgag ctcgcgcacc cgtaggtcat cggggccgat cccctcgatc
     6181 gggctgatcg ccccgcccag cacgtgatag cggccgcgga actcccgcgt cttctccacc
     6241 gccacgacgt ccttggactc ttcgacgacg cagatcacgc tgaggtcgcg gcgcgggtct
     6301 cggcagatcc ggcactgctc ctcctcggca acgttgccgc agatctcgca gaacctgacc
     6361 ttgtctttga cctcaagcag gacctgcgcc agccgcttga cgtcgaccgc atcggccgcg
     6421 agcaggtaga acgcgatgcg ctgcgcgctc ttcggaccga tgttgggcag cttgcccaac
     6481 tcgtcgatga ggtcctgaac gacaccttcg tacacgttct acagtccgag cccgggaagg
     6541 ccgcccggca aggggccgag accgccgtcg tcgaggtcgc caagaccgcc cgcctgaccg
     6601 agcccaccaa agccctgagc cagcgggccg agcttcgccg cctgcaacgc cgctgcacgc
     6661 ccagtcgcag cgcggacggc ggcgagaacc agatctgcca acgcctcggt atcggcaggg
     6721 tcaacggcct t
//
LOCUS       DATCBJ010000393         2647 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_97805, whole genome shotgun sequence.
ACCESSION   DATCBJ010000393 DATCBJ010000000
VERSION     DATCBJ010000393.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2647)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2647)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2647
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_97805"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(17..1204)
                     /locus_tag="VII50_07900"
     CDS             complement(17..1204)
                     /locus_tag="VII50_07900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009072455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HEY5050811.1"
                     /translation="MPPKLTRANHGQLVAGVARGLADHLRVNVLVIRIAFVVLTFAAG
                     AGIAMYAAFWVFVPTAPASGGQQPSRVSRLDGRGQYLAIGALTIGGVLAVQSLGLGLP
                     AELLWPLALTGFGAAVLWWQADEAQRARWRKAAVGGARPPGVASTIGGACLVVAGAAT
                     FLAYHGQLDQIRNGLLAAVVMVAGIAVITGPWWLGTLRELSAERRARIREQERAELAA
                     HLHDSVLHTLALIQRHVDDPREVQRLVRGQERELRTWLYRRPPDQQLDFGQALEAVVA
                     EVEDDHGVPIELVVVGERRIDEALSATLQAAREAMINAAKHAGTATISVYAEIEPAKV
                     TVFVRDRGRGFDPDAVPSDRLGVKESIVGRMERHGGHAVVRTAPGVGTEVRLEMSSAM
                     ARA"
     gene            1319..>2647
                     /locus_tag="VII50_07905"
     CDS             1319..>2647
                     /locus_tag="VII50_07905"
                     /inference="COORDINATES: protein motif:HMM:NF015957.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HEY5050812.1"
                     /translation="MSQDFSNPAGAPPDGNQNPGGSAPGRFDLRLLRRSRDDRMIGGV
                     CGGLGRYVGIDPIIFRIVLAALAIFGGVGLLLYALAWLLIPEDGTDLSELHRLVQGHP
                     SPAPIIAAIVVGVLGVITVTEVAYHRRGRFEVLLIVVAVIAVIAMSSRSRGMPPTTAS
                     YMPPPPYAPPPPPAKEATPSAPAYQAASYPTPGYTALGYPPPQYAAPQHSAPQYTAPL
                     GPPAGFSPSRPVYTPPPGYVPPPPKPPKLPGPPSFLGPIGVSVGVVIVGVLFALDASH
                     AIELSAQAIFATSLLIVGLALVVGTWIGRARALIAFGAILAIALALAASVGVPLRGGM
                     GNRTYAPTSTADIPATYRLAIGHQTIDLTALNLAGKKVQVTSSVGIGEVDVRVPPNVR
                     LVAHGRAGTGDVNIDGDNNGGSHVDRTLVLAAIGTAVAGEIDLDLRVGLGR"
BASE COUNT          414 a          962 c          862 g          409 t
ORIGIN      
        1 actccgttga gagccgtcat gcgcgggcca tcgctgacga catctcgagc cgcacctcgg
       61 taccgacgcc gggcgcggtg cgcacgactg cgtgcccgcc gtgccgctcc atgcgcccga
      121 cgatcgactc cttcacgccg agccgatccg acggcactgc gtcggggtcg aagccacgac
      181 cgcgatcgcg cacgaacaca gtcaccttcg cgggttcgat ctcggcatag accgagatgg
      241 tcgccgtccc tgcatgcttc gccgcgttga tcatggcctc gcgcgcggcc tgcaacgtcg
      301 ccgacaacgc ttcgtcaatg cggcgctcac cgacgacaac gagttcgatc ggtacgccgt
      361 ggtcgtcttc gacttccgcc accacagcct cgagggcttg gccgaagtcg agttgctggt
      421 cgggcggacg ccgatagagc cacgtccgca gctcgcgttc ctggccgcga accagccgct
      481 gcacttcgcg cggatcgtcg acgtggcgct ggatcagcgc caacgtatgg agcaccgagt
      541 cgtgcagatg cgcggccagt tcagcgcgct cctgctcacg gatgcgggct cgccgctcgg
      601 ctgacagttc gcgcaacgtg ccgagccacc acggcccggt gatcaccgca atgcccgcca
      661 ccatgacgac ggccgcgagc agtccgttgc ggatctgatc cagctgtccg tggtacgcga
      721 ggaacgtcgc agcaccggcg acgacgaggc atgcgccgcc gatcgtgctc gcgacccctg
      781 gtgggcgcgc accaccgacc gcggctttgc gccaacgcgc tcgctgggcc tcgtcggctt
      841 gccaccacaa aacggcagca ccgaacccgg tcagggcgag tggccacaag agctcggccg
      901 gcagaccaag accaagagac tggacggcga ggacaccacc gatggtgagc gcgccgatcg
      961 ccaggtactg accccgcccg tccaatcgac tcacccggct gggctgctga ccgccgctcg
     1021 cgggtgccgt cgggacgaac acccagaacg cggcgtacat ggcgatgcca gcaccggcgg
     1081 cgaaggtcag caccacgaag gcaatccgga tcaccagcac gttgacgcgc aggtggtcgg
     1141 cgaggccgcg cgcgacgccg gcgactagct ggccgtggtt ggcgcgggtg agtttcggtg
     1201 gcaccccacg atggtcacac gtcgcgagcg ttccggcgca gtcaccagcg caaagtcagg
     1261 gtcgagtcag ggtagttccc gatgggcgcc gccgccgcaa gccgccagga tcctcgatat
     1321 gagccaagac ttttccaatc ctgcgggcgc gccgccggac ggcaatcaga atccgggtgg
     1381 ctcggcgccc ggccgtttcg atctgcgtct gctccgtcgt agccgcgacg accgcatgat
     1441 cggcggtgtc tgcggtggcc tcggccgcta cgtcggcatc gacccgatca tcttccgcat
     1501 cgtgctggcc gcgctggcaa tcttcggcgg cgtcggcctg ctgctctacg ccctcgcctg
     1561 gctgctgata cccgaggacg gcaccgacct gtcggagctg caccggttgg tccagggcca
     1621 cccgtcccca gcgccgatca tcgcggccat cgtcgtcgga gtccttggcg tgatcaccgt
     1681 gaccgaggtc gcgtaccacc ggcgggggcg gttcgaggtc ttgctcatcg tggtggccgt
     1741 gattgcggtg atcgcgatga gcagtcgatc ccgcggtatg ccgccgacta ccgcttcgta
     1801 catgccaccg cccccgtacg ccccgccgcc gccacctgcc aaggaggcta cgccgtcggc
     1861 acccgcgtac caagccgcga gttatccaac gcccggctac accgcgcttg gctacccgcc
     1921 gccgcaatac gcggcaccgc aacacagcgc cccgcagtac acggcgccgt tgggtccacc
     1981 ggccggcttc tcgccgtccc gcccggtgta cacgccgccg ccgggatacg tgccgccacc
     2041 gccgaagcca cccaagttgc caggcccgcc gtcgttcctt gggcccattg gcgtcagcgt
     2101 cggcgtcgtc atcgtcggcg tgctgttcgc actcgacgca tcgcacgcga tcgagctcag
     2161 tgctcaggcg atcttcgcga cgtcgctgct catcgtcgga ctcgcactcg tcgtcgggac
     2221 atggatcggc agagcgcgcg cgctcatcgc gttcggcgcc atcctggcaa tcgcccttgc
     2281 cctcgcggct tcggtcggcg tgccgctgcg gggcggcatg ggcaaccgga cctacgcgcc
     2341 gacgtcgacc gccgacattc ccgcgacgta tcgcctcgca atcgggcacc agacgatcga
     2401 tctgaccgct ctcaacctcg ccgggaaaaa ggttcaggtg acctcgagcg tcggcatcgg
     2461 ggaggtcgac gtccgggtgc ctccgaatgt caggcttgtc gcccacggtc gcgccggaac
     2521 aggagacgtg aacatcgacg gcgacaacaa cgggggaagc catgtcgacc gcacgctcgt
     2581 gctggccgcg atcgggacgg cggtcgctgg cgagatcgac cttgacctgc gcgtcgggct
     2641 aggccgg
//
LOCUS       DATCBJ010000394         2077 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_114032, whole genome shotgun sequence.
ACCESSION   DATCBJ010000394 DATCBJ010000000
VERSION     DATCBJ010000394.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2077)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2077)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2077
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_114032"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1648)
                     /gene="mtrB"
                     /locus_tag="VII50_07910"
     CDS             complement(<1..1648)
                     /gene="mtrB"
                     /locus_tag="VII50_07910"
                     /inference="COORDINATES: protein motif:HMM:NF040691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MtrAB system histidine kinase MtrB"
                     /protein_id="HEY5050813.1"
                     /translation="MLRRVTRRWRRSIQVRVVSSTLVVSAIVVALLGFVVLQQVKQGL
                     IQAKLRSSVAEEQLGVSNARTLLSSDAGSLDVQALLQEVVQALSPIQGGAEGGYEVVG
                     QEVVTGTQSKTQLALNNGLLASSVPDRLTKALNGDSQYYYTYTRLVYDKGARSPGSAI
                     HNYSSYPGLVIGSSMQVPEGSFNTYQFYYLFSLQQPQQTLALVWRTLMLSGIALVVLL
                     AGIAWLVTRQVVTPVRMAARIAERLADGRLQERMKPKGEDDLARLAASFNKMADSLQR
                     QIRRLEELSRIQRRFVSDVSHELRTPLTTVRMAADVLHEARSDFDPASARSAELLQTQ
                     LDRFESLLGDLLEISRHDAGAASLEADALDVRDLVRRVVDATEPLAVRRGSVVAVHMP
                     DEPCICEADGRRVERILRNLLVNAAEHGEGSPIDVYVVADAAVVAIAVRDHGIGLRPG
                     ESSLVFNRFWRADPARARTTGGTGLGLSIASEDARLHGGRLQAWGERGKGSQFVLTLP
                     RQAGSIVTASPIPVEPPDATHEETQTTTNGTTAAKPASHHP"
     gene            complement(1761..>2077)
                     /locus_tag="VII50_07915"
     CDS             complement(1761..>2077)
                     /locus_tag="VII50_07915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005316841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HEY5050814.1"
                     /translation="EPAAETIAIGDLSIDVAGHAVSRSGGQIPLTPLEFDLLVALAKK
                     PRHVFSREVLLEQVWGYRHAADTRLVNVHVQRLRSKVELDPERPEIVVTVRGVGYKAG
                     PA"
BASE COUNT          372 a          671 c          673 g          361 t
ORIGIN      
        1 cagggtgatg ggaggcaggc ttcgcggcgg tcgtcccgtt agtcgtcgtc tgcgtctctt
       61 cgtgggttgc atctggcggc tcaactggga tcggcgaggc tgtgacgatc gagccagctt
      121 gacgaggcaa ggtgagcacg aactgtgagc ctttcccgcg ttcgccccaa gcctggagcc
      181 ggccgccgtg caatcgggcg tcttcagatg cgatggaaag gccgaggccg gtgccacccg
      241 tcgtccgcgc tcgggccggg tctgcgcgcc agaacctgtt gaacaccagc gacgattcgc
      301 ccggtcgcaa gccgatgccg tgatcacgca ccgcgatagc cacgacagcg gcgtctgcca
      361 cgacgtacac gtcgatcggg ctgccctcgc cgtgctcggc ggcgttgacc agcagattgc
      421 gcaggatccg ttcgacacgg cgtccatctg cctcgcagat gcatggttcg tcaggcatgt
      481 gcaccgccac gacactgccg cggcggacag cgagtggctc ggtcgcgtcg acaactcgac
      541 ggacgagatc ccggacgtcg agtgcgtcag cttcgaggga cgccgcgcct gcgtcgtgac
      601 gactgatctc cagcaagtcg ccgagcaacg attcgaagcg atcgagttgg gtctggagaa
      661 gttcggctga tcgtgcactc gcagggtcga aatccgacct tgcctcgtgc aagacgtcgg
      721 cagccatgcg cacggtcgtc aagggtgtcc gcagttcgtg cgacacgtcg gaaacgaacc
      781 ggcgctggat acgcgacagc tcttcgagcc gccggatctg gcgttgcaga ctgtcagcca
      841 tcttgttgaa ggacgcggcg agtcgcgcga ggtcgtcctc gcccttgggt ttcatccgtt
      901 cttgcaaccg tccgtcggca agccgttcgg cgatgcgcgc cgccatccgc acgggcgtca
      961 cgacttggcg ggtcaccagc cacgcgatac cggcaagcag cacaaccagc gcgatcccgc
     1021 tcaacatcaa tgtgcgccag accagcgcga gtgtctgctg cggctgttga agggagaaca
     1081 gatagtagaa ctggtaggtg ttgaagctgc cctcgggcac ctgcatcgag ctgccgatta
     1141 ccagccccgg gtaggacgaa tagttgtgga tggcgctgcc cggcgaacgc gcacccttgt
     1201 catacacgag ccttgtgtat gtgtagtagt actgcgaatc tccgttcaac gccttggtca
     1261 gccggtctgg cacgctgctc gccaacaggc cgttgttgag tgcaagttga gttttcgact
     1321 gcgtaccggt gacgacctcc tggccgacta cctcgtagcc gccttccgct ccgccctgta
     1381 tcgggctcag agcctgcacg acttcctgga gcaacgcctg gacgtcgaga gagccagcgt
     1441 cgctagacag cagcgtccgc gcgttgctga caccgagctg ttcctcggcg accgacgacc
     1501 gcaacttggc ctggatcagt ccttgcttga cctgctgcag caccacaaaa ccaagcaggg
     1561 cgacgacgat cgccgacaca acaagcgtgc tgctgacgac tcggacctga atcgatcgcc
     1621 gccagcgccg ggtgactcgc cgcaacaccc gccggactct tcggcgaacg cggctggcga
     1681 ggctacgcag aggaccgcga ccggacagcg tcaaccgcct gggcggcggg aggcgggcag
     1741 ggggcgacgg atgcagaccg tcacgccggt cctgccttgt aaccgacgcc gcgcacagtc
     1801 acgacgatct cgggacgctc ggggtcgagc tcgactttcg accgcagccg ctgcacatgc
     1861 acgttgacca aacgggtgtc agccgcgtgc cggtaacccc acacttgctc gagcagcacc
     1921 tcgcgggaga acacgtggcg cggtttcttt gcgagtgcga cgagcagatc gaactcgagc
     1981 ggcgtcaacg ggatctgccc accactgcgg ctcactgcgt ggccagcaac atcgatcgag
     2041 aggtcgccga tggcaatggt ctcggcggct ggttcgt
//
LOCUS       DATCBJ010000395         2601 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_342093, whole genome shotgun sequence.
ACCESSION   DATCBJ010000395 DATCBJ010000000
VERSION     DATCBJ010000395.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2601)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2601)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2601
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_342093"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1022)
                     /locus_tag="VII50_07920"
     CDS             complement(<1..1022)
                     /locus_tag="VII50_07920"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HEY5050815.1"
                     /translation="MDTVDVPGAVYLDHAATTPMVPAAVAAMTEQLRVVGNPSSLHRA
                     GRTARRVVEESRETIAQALGARPSEVVFTSGGTEADNLAVKGLFWARRAADPRRVRIL
                     ASAVEHHAVLDCVEWLAGHDGAEPVWLDVDGLGRVRPETVRAAIESDPLSVALVTVMW
                     ANNEVGTVQPIADIVAIAHSYGIPVHSDAVQAVGQLPIDFAASGLDALTVSGHKVGGP
                     LGVGALIVARDLSLTPVLHGGGQERDLRSGTLDTPAIAGFAAAVCDAVALQPARAAAL
                     VELREDLIRRVLTAVPDAVLNGDPGVRTGERLPGNAHFGFPGCEGDALLMLLDARGVE
                     CSTGSA"
     gene            1109..1525
                     /locus_tag="VII50_07925"
     CDS             1109..1525
                     /locus_tag="VII50_07925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5050816.1"
                     /translation="MTAEQTLAAQHPCPCGIAPASQVLRCVACPARGRRATDGELMSA
                     QERRTTDPDAVIPLMSYSRWKKTDGTFGPAGRIIATVLLVVPLPVLALGVATGIGIIG
                     AGLYVIIVMPWALRDIWKRGATHVQPPLPAPGRPQF"
     gene            1602..>2601
                     /locus_tag="VII50_07930"
     CDS             1602..>2601
                     /locus_tag="VII50_07930"
                     /inference="COORDINATES: protein motif:HMM:NF025617.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4350 domain-containing protein"
                     /protein_id="HEY5050817.1"
                     /translation="MSQPLLSPSVSRARRAFPSVLFDEAHSEAWSIRAETVAQMNPRH
                     PGDAGYLKAAAALRERGMVVDSHISGPLTAAALADRDVVIVAHPSDGTWERVTGIGSA
                     LLAPAEIDTLESFVRDGGGLVVLAECEQDKYGNNLVNLLERFGVRPMNVTVQDTEHNH
                     QDVVAWVRATLPRQRGKADLLARVSGACFYRSGVLSITNPDAAVLAKTSPSADPPDQP
                     LAVALQFGLGRLVVFADSDLFGDDSMGDYDHLTLWTNAITWAASGAVLAGAAASASWL
                     LEHPGWVALKESVGALRGLQAKDGSLDVSTHGESAGTAATAEVDTVVSAIEALRPHF"
BASE COUNT          403 a          944 c          869 g          385 t
ORIGIN      
        1 cacgccgagc cggtcgagca ctcgaccccg cgcgcgtcga gcagcatcag caaggcatcg
       61 ccttcgcagc cagggaagcc gaagtgcgcg ttgcccggca gccgctcgcc agtgcgaacg
      121 ccggggtcgc cgttcaatac cgcatcgggg acggcggtca gcacccgccg gatcaaatcc
      181 tcacgcagct ccacgagagc ggccgcacgc gccggctgca acgcgaccgc gtcgcacacc
      241 gccgcggcga acccggcgat cgccggggtg tcgagcgtgc ccgaacgcag atcgcgctcc
      301 tgcccgccgc catgcaagac cggtgtgagt gagaggtcgc gggccacgat caacgccccg
      361 acgccaagcg ggccgccgac cttgtgcccg gagacagtca gcgcgtcgag accgcttgcg
      421 gcgaagtcga tcggcagctg accgacggcc tgcaccgcat cggagtgcac ggggatgcca
      481 tagctgtgcg cgatggcgac gatgtcggcg atcggctgca cagtgccgac ctcgttgttc
      541 gcccacatca ccgtgaccag ggcgaccgat aaaggatcgc tctcgatcgc cgcacgcacg
      601 gtctcgggac gcacccgccc cagcccatcg acgtccagcc agaccggctc ggcgccatcg
      661 tggccagcga gccactcaac gcaatcgagc accgcgtgat gctcgacggc gctggccagg
      721 atccgcaccc ggcggggatc ggcggcacgg cgtgcccaga acaggccctt gaccgcgagg
      781 ttgtcggcct cggtaccgcc ggaggtgaag acgacctcgc tcggacgcgc cccgagcgcc
      841 tgggcgatcg tctcgcgaga ctcctcgacc acccgccggg cggtccgacc agcccggtgc
      901 agcgacgagg ggttgccgac cacgcgcagc tgctcggtca tggcggcaac ggccgccggc
      961 accatcggag tggtggccgc gtggtcgagg tatacggcgc caggcacgtc gacggtgtcc
     1021 atcgccgcca gcctagccgt tcggagacct tcaaggttcc gtcaaatcag gcctcaacgc
     1081 ccgaccccaa gttgccgatg acacgtccgt gacggccgag cagactttgg cagcccagca
     1141 cccgtgcccg tgcggaatcg caccggccag ccaggtgctg cgctgcgtgg cgtgccctgc
     1201 ccgcggtcgg cgcgcgacgg acggcgaact catgtcggcc caggagcgcc ggacgacaga
     1261 ccccgacgcg gtcatccccc tcatgagcta ttcgcgctgg aagaagacag atggcacgtt
     1321 cggcccggcc ggccggatca tcgccaccgt tctcctcgtc gtcccgctgc ctgtcctcgc
     1381 cctcggcgtt gcgaccggaa tcgggatcat cggcgcgggt ctctacgtca tcatcgtgat
     1441 gccgtgggcg ttgcgcgaca tctggaagcg cggcgcgacc cacgtccagc cgccgctgcc
     1501 ggcaccggga cgcccgcagt tctgacgtcc gcgaccgcgc agtccggccg gtctcgccgc
     1561 gccggtctgt aaggccgcac cggctctgta ggctgatcgt tgtgagccag cctttgttgt
     1621 caccctctgt ttcccgcgcc cgtcgcgcgt ttccgagcgt gttgttcgac gaggcccata
     1681 gcgaagcgtg gagcatccgg gccgagacgg tcgcacagat gaacccgcgg caccccggcg
     1741 acgccggcta tctcaaggcc gccgccgcat tgcgcgaacg cggcatggta gtcgactcgc
     1801 acatctcggg accgctgacc gcggccgcat tggccgatcg cgacgtcgtg atcgtggcgc
     1861 atccctccga cggcacctgg gaacgggtca ctgggatcgg atcggcattg ctcgcgcccg
     1921 ccgagatcga cacgttggag tcgttcgttc gcgacggcgg cgggctcgtc gtgctggccg
     1981 aatgcgagca ggacaagtac ggcaacaacc tggtcaacct gctcgaacgg ttcggcgtcc
     2041 ggccgatgaa cgtcaccgtt caagacaccg agcacaacca ccaggacgtc gtggcctggg
     2101 tccgcgctac gttgccgcgc cagcgcggca aagccgacct gctggcccgc gtctccggtg
     2161 cgtgcttcta ccgctccggc gttctttcca tcactaaccc ggacgccgcg gtgctggcga
     2221 aaacctcgcc ttccgccgac ccgccggacc agccgctggc ggtcgccctg cagttcgggc
     2281 tcggccgcct cgtggtgttc gccgattccg acctgttcgg cgacgattcg atgggcgact
     2341 acgaccacct gacgctctgg accaacgcga tcacctgggc agcctcgggt gccgtcctcg
     2401 cgggtgctgc cgcgtcggcg agttggctgc tcgagcaccc cggctgggtg gcgctgaagg
     2461 aatcggtggg cgcacttcgc gggctgcagg caaaggacgg ctcgctcgac gtgtcgaccc
     2521 atggcgagtc ggcgggcacg gccgcgaccg ccgaagtcga caccgtcgtt tcggcgattg
     2581 aagcgttgcg gccgcacttc g
//
LOCUS       DATCBJ010000396         3475 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_451213, whole genome shotgun sequence.
ACCESSION   DATCBJ010000396 DATCBJ010000000
VERSION     DATCBJ010000396.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 3475)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3475)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3475
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_451213"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..411)
                     /locus_tag="VII50_07935"
     CDS             complement(<1..411)
                     /locus_tag="VII50_07935"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009072889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008725 - DNA-3-methyladenine glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HEY5050818.1"
                     /translation="MTAVVGPDGLRRCNWAHSAPEYIVYHDNEWGRAVHGDDAVFERL
                     VLEAFQSGLSFITILRKREAFRAAFAGFSIGAVAQFTEGDAERLMADAGIVRNRAKIA
                     AGMANARAAAAVPEGLSELVWSFAPTRRRAAPKND"
     gene            complement(408..908)
                     /locus_tag="VII50_07940"
     CDS             complement(408..908)
                     /locus_tag="VII50_07940"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEY5050819.1"
                     /translation="MAELVASADVDACVERVWAALTDWDTHGDWMVATTVQRTTSDGD
                     GVGAGIEGITKLGPFAVHDTMTFSQWQPPPASPARCVVEHTGTLVRGSGAFEVEDLGD
                     GRSRVVWSEWVQLPLGLLGETGWLVVRPVVAVFLRLSLRRLARLVEATEGSDGVEAAE
                     AVGDKP"
     gene            complement(917..1525)
                     /locus_tag="VII50_07945"
     CDS             complement(917..1525)
                     /locus_tag="VII50_07945"
                     /inference="COORDINATES: protein motif:HMM:TIGR03544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HEY5050820.1"
                     /translation="MPLLFGILVSAGVIFAIFAVTVGRGGSMTQFQPDWPGQALPQDR
                     PVRASDLADAKFSVAFRGYRMAEVDEALDRLAVEIAQRDERIQQLTGQPFDGAPPVAL
                     AAASDPAVHQPGAYDTAVYQPHAQDPYAQTPNAHDPNATPYAYDPGVYQPPQPPAEPP
                     AYQPHGYPPGVLDGGGHPTQPIPPATDPGTTDPGTAHPGTEG"
     gene            1603..2466
                     /gene="folP"
                     /locus_tag="VII50_07950"
     CDS             1603..2466
                     /gene="folP"
                     /locus_tag="VII50_07950"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HEY5050821.1"
                     /translation="MPLRLGQREFADNEFAVMAIINRTPDSFFDRGATFATEAALAAI
                     DQALADGADIVDIGGVKAGPGPDVDTAEEIERVADFVALVRKKHPTLVISVDTWRAEV
                     GRAVAAAGADLLNDAWGGHEPELAQVAASAGIGLVCSHAGGLPPRTDPHRVSYDDVVS
                     DVIGTVTGLARRAVSVGVRADGILIDPAHDFGKNTRHSLEVSRRLDELVATGWPVLVA
                     LSNKKFVGETLDLPAGERLTGTLAATAVAAWQGARVFRAHQVRQTRQALDMIASIRGY
                     RAPAVARRALA"
     gene            complement(2490..3053)
                     /locus_tag="VII50_07955"
     CDS             complement(2490..3053)
                     /locus_tag="VII50_07955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016460836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HEY5050822.1"
                     /translation="MTEIRLQQRLNSLSALENLDLLGEPVVTALRALPSEIAAAVTVT
                     EIDPALADTAAFCARYGVLADESANCVVVAGRRGEVTTLAACVVLATMRADVNGLVRR
                     TLDVRKISFASMDLAVAASGMEYGGITPIGLPVEWPLFIDRAVVAAGDVVVGSGVRRS
                     KIVVAGSTLAALPNATVLDDLGLPAAH"
     gene            complement(3050..>3475)
                     /locus_tag="VII50_07960"
     CDS             complement(3050..>3475)
                     /locus_tag="VII50_07960"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0009691 - cytokinin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LOG family protein"
                     /protein_id="HEY5050823.1"
                     /translation="AGGGHTTGVIPQALIDMEVADGDADELVVTRDLRERKGEMERRS
                     DGFLALPGGLGTLDEIFEIWVSRTLGMHDLPLVVLDPDGVFAMMRAQVDDLVLRGFAR
                     PAVRDAIHWATGVGEAFDLLEAQQMRIVPTAGEVLESDP"
BASE COUNT          585 a         1204 c         1146 g          540 t
ORIGIN      
        1 atcgttcttc ggcgcggccc gccgccgcgt cggcgcgaac gaccacacca gctccgacaa
       61 gccttcgggc acggccgcgg cggcgcgcgc attggccatg ccggcggcga tcttggcccg
      121 gttgcggacg atgcctgcgt cggccatcag ccgctcggcg tccccttcgg tgaactgcgc
      181 aaccgccccg atcgagaacc cggcgaacgc cgcccgaaac gcctcccgct tgcgcaggat
      241 cgtgatgaac gacaacccgg attgaaaggc ttcgagcacc agccgctcga acaccgcgtc
      301 gtcgccgtga acggcgcggc cccactcgtt gtcgtggtag acgatgtatt cgggagcgct
      361 gtgggcccag ttgcaccggc gcaggccgtc cggtccgacg accgccgtca cggtttgtcg
      421 ccgacggctt cggccgcctc gaccccatct gacccttccg tcgcctcgac gagtcgcgcg
      481 agtcggcgaa gtgacagccg cagaaacacc gcgacaaccg gccggaccac gagccaaccc
      541 gtctcaccca gcaggccgag tgggagttgc acccactccg accagacgac gcgcgaacga
      601 ccgtcgccga ggtcttcgac ctcgaaggcc ccggagccac ggaccagcgt gccggtgtgc
      661 tcgaccacgc accgggctgg gctcgccggc ggcggttgcc actgcgagaa cgtcatcgtg
      721 tcgtgaaccg caaacgggcc gagcttggtg atgccttcga tgcccgctcc gacgccgtca
      781 ccgtcgctgg tcgtgcgctg cacggtggtg gccaccatcc agtcgccgtg cgtgtcccag
      841 tcggtgaggg ccgcccagac ccgctcgacg caggcatcga catcggccga cgccaccagc
      901 tcggccatgg cggtacctag ccctccgtac cggggtgggc tgtaccgggg tcggtcgtac
      961 cgggatcggt cgccggcggg atcggctgcg tcgggtgccc accaccatcg aggacgccgg
     1021 gtgggtagcc gtgcggttgg taggcgggcg gctcagctgg cggctgcggt ggctgataca
     1081 cgcccgggtc gtacgcatat ggggtcgcgt tggggtcgtg cgcgttcggg gtttgcgcgt
     1141 aggggtcttg cgcatggggt tggtacacgg cggtgtcgta ggcacccggc tgatgcaccg
     1201 cgggatccga tgccgccgcg agtgcaacgg gcggcgctcc atcgaacggc tggccggtca
     1261 gctgttggat ccgctcgtca cgctgcgcga tctcgacggc cagccggtcg agtgcctcgt
     1321 cgacctcggc catccgataa ccgcgaaatg caacgctgaa cttcgcgtca gccaggtctg
     1381 acgcacgcac cgggcggtct tgcggcagcg cttgcccggg ccaatcgggt tgaaactggg
     1441 tcatcgaacc gccacggcca acggtgaccg cgaagatcgc gaagatgact ccggcagaga
     1501 ccagaatccc gaacagaagg ggcacccccc gatcgtgcca tgcctgcctg accggaggat
     1561 ggcaggcacc ccgtctgatc acgggagatc cctggagcgc caatgccgtt gcgactaggt
     1621 cagcgcgagt tcgccgacaa cgagttcgcc gtcatggcca tcatcaaccg gacccccgat
     1681 tcgttcttcg atcggggtgc gaccttcgcg accgaggcgg cactcgccgc gatcgaccag
     1741 gcgctcgctg atggcgcgga catcgttgac atcggaggtg tcaaggcagg accagggccg
     1801 gacgtcgata ctgcggagga aatcgagcgt gtcgccgatt ttgtcgcgct ggtaaggaaa
     1861 aagcatccaa cgcttgtcat cagtgtcgac acctggcgag ccgaggtcgg ccgcgccgta
     1921 gccgccgccg gtgccgacct gctcaacgac gcgtggggcg gccacgaacc cgaactcgcc
     1981 caggttgcgg cgtccgccgg tatcggcttg gtgtgctcgc acgccggcgg attaccacct
     2041 cgtacggatc cgcaccgggt gagctacgac gatgtcgtga gtgacgtgat cggcacggtg
     2101 accggtttgg cgcggcgggc ggtgtctgtc ggtgtgcgcg cagacggcat cctcatcgat
     2161 ccagcgcacg atttcggcaa gaacacccgg cattccctgg aagtcagccg ccggctcgac
     2221 gagttggtcg cgacgggttg gccggtgctc gtcgcgttgt cgaacaagaa attcgttggg
     2281 gagaccctcg acctccccgc cggcgagcgg ctaaccggga ctctcgcggc gaccgcggtg
     2341 gccgcttggc agggtgctcg cgtcttccgt gcgcatcagg ttcgccagac ccggcaggcg
     2401 ctcgacatga tcgcgtccat ccgcggctac cgggcgcctg cagtcgcacg gcgcgcactc
     2461 gcctgaaagc tgacgccgcg acccgatgac tagtgcgcgg ccggcaggcc gagatcatcg
     2521 agcacggtgg cattcggcaa tgcggccagc gtcgagccgg cgaccacgat cttcgaacgt
     2581 cgcacaccac tgccgaccac cacgtcgccc gcggcgacga ccgcccgatc gatgaagagt
     2641 ggccactcca cgggcagccc gatcggggtg atgccgccgt actccatgcc ggacgccgcc
     2701 accgccaggt ccatcgaagc gaacgagatc ttccggacgt cgagtgttcg gcgaaccagt
     2761 ccgttaacgt ccgcccgcat cgtggcgagc accacgcacg cggccagtgt cgtcacctca
     2821 ccacgacgtc ccgcaacgac aacgcagttc gccgactcat cggccagcac gccgtatcgc
     2881 gcgcagaatg ctgccgtatc ggcaagtgcg ggatcgatct cggtgactgt gaccgcggcc
     2941 gctatctcgg agggcaatgc gcgcaatgcg gtcaccacgg gttcgccgag caggtcgagg
     3001 ttttccagtg cagacagtga attcagccgc tgttgcaacc ggatctcggt cacggatccg
     3061 actccagaac ttcgccggcg gtcggcacga tacgcatctg ctgcgcctcc agcagatcga
     3121 acgcctcacc caccccggtg gcccaatgga tcgcgtcgcg aaccgctggc ctagcaaaac
     3181 cacgcagcac gagatcgtcg acctgcgcac gcatcatcgc gaagacaccg tcaggatcaa
     3241 ggacgacgag gggcaggtcg tgcatgccaa gcgtccgcga cacccagatc tcgaagatct
     3301 cgtcgagggt gccgagaccg ccaggcaagg ccaggaatcc gtccgatcga cgttccatct
     3361 cgcctttgcg ttcgcgcaga tctcgcgtga cgaccagctc gtcggcgtca ccgtcggcga
     3421 cctccatgtc gataagggcc tgcggaatca ccccagtcgt gtgcccgccc cccgc
//
LOCUS       DATCBJ010000397         2311 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_156898, whole genome shotgun sequence.
ACCESSION   DATCBJ010000397 DATCBJ010000000
VERSION     DATCBJ010000397.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2311)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2311)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2311
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_156898"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..671
                     /locus_tag="VII50_07965"
     CDS             <1..671
                     /locus_tag="VII50_07965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0018142 - protein-DNA covalent
                     cross-linking [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HEY5050824.1"
                     /translation="EVYAVVERIDKQQPSAPPQRQLRVVRWGLVPSWARDRKIASKLI
                     NARVETLTEKPAFRKAFAVRRCLLPADGYYEWYTDDATDGAADKPGGKTRKQPYFIRG
                     RGGGILPMAGLYEFWRDPHAEPDVDPWLWSATVITTEATDDVGRIHDRMPMLVEPQNW
                     ARWLDPREDDTDAIAHLLVPAAAGRLEAYPVSTEVNDVRNDGPQLIEPISVSHDAVNA
                     HSDG"
     gene            744..1100
                     /locus_tag="VII50_07970"
     CDS             744..1100
                     /locus_tag="VII50_07970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HEY5050825.1"
                     /translation="MASTVPSEAQTRARLEEMVAELDRSVATLRIEPPDPDEGGTPGD
                     ADAGGDLADNARSAAVIEAAEEQRAHVQEALRRLDAGEYGRCVDCGTELSAARLEAKP
                     EAARCVTCQGKAESTR"
     gene            1070..1714
                     /locus_tag="VII50_07975"
     CDS             1070..1714
                     /locus_tag="VII50_07975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009082971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta family hydrolase"
                     /protein_id="HEY5050826.1"
                     /translation="MPGKGREHSLIRVPTEVGMAEAELARPIAVPTGLLVLGHGAGGS
                     IDAPDLRAVTSAALEVGIAVARVRQPYLVAGRRAPAPAAQLDIAWTAVVEHLGAVLLA
                     QWGGLRVAVGGRSSGARVACRTADATGAVGILALAFPLHPPGHPEKSRAAELEVGVPL
                     LVLQGTRDPFGGPDQFPPTVAVQEVSGADHRLAGPALADAVSVAVGWLSATLVG"
     gene            1805..>2311
                     /locus_tag="VII50_07980"
     CDS             1805..>2311
                     /locus_tag="VII50_07980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEY5050827.1"
                     /translation="MHTAHELTARENSPLRWPAWSVSSRPVVSMSDPAGSAREQLIGT
                     AQGARSLATVEESTDERSARFEQSAMPYLDQLYSAALRMTRNPSDAEDLVQETFAKAF
                     AAFHQFEQGTNLKAWLYRILTNTFINSYRKKQREPKQTGGDELEDWQLARSAEHTSSG
                     LRSAETEAL"
BASE COUNT          408 a          742 c          827 g          334 t
ORIGIN      
        1 atgaggtcta tgcagtggtg gagcgcatcg acaagcagca gccaagcgcg ccaccgcaac
       61 gccagctgcg cgtcgttcgc tggggtctgg tgccttcgtg ggcgagggat cgcaagatcg
      121 cgagcaagct catcaacgcg agggtcgaga cgctgaccga gaagcccgcc tttcggaaag
      181 ccttcgcggt gcgtcgttgc ttgttgcccg ccgacggcta ctacgagtgg tacaccgacg
      241 acgcgacgga cggtgcagcc gataagccag gcggcaagac gcgcaagcag ccgtacttca
      301 ttcggggtcg tggtggcggg attctgccga tggcgggcct gtacgagttc tggcgtgatc
      361 cacatgctga gccggacgtc gatccgtggc tgtggtcggc gactgtgatc actactgagg
      421 caaccgacga cgtggggcgt atccacgacc ggatgcccat gttggtcgag ccgcagaact
      481 gggcacgctg gctcgatccg cgcgaagacg acaccgacgc gatcgcgcac ttgctcgtgc
      541 cggcggcggc cgggcgcctc gaggcgtacc cggtctcgac agaggtcaac gacgtgcgaa
      601 acgacggtcc gcagctgatc gagccgatat cggtcagcca cgatgcggtg aatgcacact
      661 ctgatgggta gggggagcag atcgggtcca accgagcagc ggccgatcac ccaaatggac
      721 gactacacac tgaggggacg tcgatggctt ccacagtgcc gtcagaggcg cagactcgag
      781 cacggctcga ggagatggtg gccgagctcg accgatcggt cgccactttg cgcatcgaac
      841 cgccagaccc cgacgagggc ggcacgcccg gggacgccga cgcaggtggc gacctggccg
      901 acaacgcgcg ctcggccgcc gtcatcgagg cggctgagga acagcgggcg cacgtacaag
      961 aagcgttgcg gcgtctcgac gccggtgagt acgggcgttg tgtcgattgc gggacggagc
     1021 tgtcggcggc ccgcttggag gccaagcctg aagctgcgcg gtgcgtgacg tgccagggaa
     1081 aggccgagag cactcgctga ttcgggtgcc gaccgaggtc ggcatggcgg aggccgaact
     1141 cgcacgtccc atcgccgtcc cgaccggctt gctggtgctg ggacacggag cgggcggcag
     1201 tatcgacgcg ccggatctgc gcgcggtgac ctcggccgca ctcgaggtgg gaatcgcggt
     1261 ggcgagggtg cggcagccct acctcgtcgc cggccgtcgc gcgcccgcgc ccgcggcaca
     1321 gctcgacatt gcctggacgg cggtggtcga acaccttggc gccgtgctgt tggcccagtg
     1381 gggcggactg cgcgtggcgg tcggcggccg cagcagcgga gcacgggtgg cctgccgaac
     1441 ggcggacgca accggggctg tcggcattct tgcattggcc ttcccgctgc atccaccggg
     1501 tcatccagag aagtcccggg ctgccgagct cgaggtcggc gtcccgctgc tggtcctcca
     1561 aggaacgcga gacccgttcg gaggaccgga ccagttcccg ccgacggtcg ctgtccagga
     1621 agtaagcggc gccgaccacc ggctcgcggg accggcgctg gccgacgcgg tgagcgtcgc
     1681 ggtcggctgg ctgtcggcca ccctcgtcgg ctgagcgtgg agtcgggcgg gggaatcgcc
     1741 ccgcagccga ccgcgttgtg aactctcgac agccagcgag cgattcgaga gggaggcgac
     1801 gcaggtgcac acggcgcacg aactcacggc tcgcgagaac tccccgctgc gatggccggc
     1861 gtggtcggtg tcgagccgac cggtagtgtc gatgagcgac cccgctgggt ccgcacgcga
     1921 gcaattgatc ggcacagcac agggagcgcg gtctttggcc accgtcgagg aatcaaccga
     1981 cgaacgcagc gcgcgcttcg agcaaagcgc gatgccctac ctcgaccagc tctattcggc
     2041 cgcgctgcga atgacgcgta acccctccga cgcggaagac ctcgtgcagg aaacgttcgc
     2101 caaggcgttt gccgccttcc accagttcga acagggcacc aacctcaagg cgtggctgta
     2161 ccggatcctg acgaacacct tcatcaacag ctaccgcaag aagcagcgcg agcccaagca
     2221 gactggtggc gacgagctcg aagactggca gctcgcccga tcggccgagc acacgtcgtc
     2281 cggattgagg tcggcagaga cggaggccct c
//
LOCUS       DATCBJ010000398         3895 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_381533, whole genome shotgun sequence.
ACCESSION   DATCBJ010000398 DATCBJ010000000
VERSION     DATCBJ010000398.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 3895)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3895)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3895
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_381533"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2056)
                     /locus_tag="VII50_07985"
     CDS             complement(<1..2056)
                     /locus_tag="VII50_07985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G-like protein EF-G2"
                     /protein_id="HEY5050828.1"
                     /translation="MSDKATGPAGAAGTAPTADQPSKIRNVVLVGHSGAGKTTLVEAL
                     LVATGAIPRAGRVEDGTTTTDFEDAEVRHQRSVSLALAPVEHDGVKINLLDAPGYADF
                     VGDLRAGLRAADAALFVMSAVDGIDGSTRMLWEECAQVGMPRAVVITKLDSPRADFEA
                     VVGACQATFGNGVLPLYVPVQAADGTMTGLLGLLSRQVGDYSSGRREVRPADAAELEA
                     IGGTRDAVLEGIIAESEDETLMDRYLAGEDIDVKIVIEDLEKAVARGSFYPVLGVCGT
                     TGIGSAELLELLAHAFPSPLEHPLPPVMPLYGSKITTLTCDPAGPLAAEVVKTTTDPY
                     VGRVSLVRVFSGTLRPDTVVHVSGHGMADRGHEDHDVDERVGTLSSPLGKTHRPVTQC
                     IAGDICAIGKLARAETSDTLSDKENALLLSPWDMPEPLLPIAVRAKSKSDEDKLSQGL
                     GRLAAEDPTLRLEMNAETHQLVLWCIGEAHAEVLLDRLKNKYGVEVEQVPLRVPMRET
                     FGSKVVAHGRHVKQSGGHGQFGVCDIEIEPLPQGTGFEFIDKVFGGSVPRQFIPSVEK
                     GVRAQMERGVAAGYPLVDVRVTLTDGKAHSVDSSDMAFQMAGALAMKEAAKTGQILLL
                     EPVDQVNVLVGDDYVGAVMSDLSGRRGRVTGTEPIGTGRTLIHAEVPQVEISRYAIDL
                     RSL"
     gene            2282..2917
                     /locus_tag="VII50_07990"
     CDS             2282..2917
                     /locus_tag="VII50_07990"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HEY5050829.1"
                     /translation="MLKRARIPLQRVLGPLGQALARAGVSPNVVTALGALGVCASALI
                     CYPRGWLFWGSFAVTIFVLSDLVDGALARARGITGPWGAFLDSSLDRVADSAVFAGLI
                     WWFAREGKPWLLGLCIFCLVAGNLVSYTRARAEGLGLRADVGVAERAGRTIVILVATG
                     VSGLGVPFIQAVGLWLLAVASAITVGQRFAVVYRQATSSVTPASITPAQDS"
     gene            2914..3846
                     /locus_tag="VII50_07995"
     CDS             2914..3846
                     /locus_tag="VII50_07995"
                     /inference="COORDINATES: protein motif:HMM:NF005919.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylinositol mannoside acyltransferase"
                     /protein_id="HEY5050830.1"
                     /translation="MTDARVSGRDRWQTRGFVAGATLVRVLPERVLRPVFRLICDAIW
                     RRHGRSVQQLERNLRRVVGDAVPTSELAVLTRRAMRSYGRYWLEFFRLPVFSRARILD
                     RMIFEGEEFLSGALAQGRGVVLALPHSGNWDHAGAWLVLRGYPFTTVAERLKPEELFD
                     RFVAAREKVGMEVLPLTGGGRTTFALLLARLRAGKTLCLVAEREFGDGGIEVSFFGEP
                     AMMPAGPASLALATGAALLPAYLWYSDEGGPGGTWCGWMREEIKPPAEGDKRAKITAM
                     TQALADRFAEGIAAHPQDWHMLQPMWLADVQARG"
BASE COUNT          563 a         1288 c         1321 g          723 t
ORIGIN      
        1 agagagaccg caggtcgatc gcgtacctgg agatttcgac ctgcggaacc tctgcgtgga
       61 tcagcgttcg ccccgtaccg atcggctcgg ttccggtcac ccgtccgcgg cgtccggaca
      121 gatcgctcat caccgcgccg acgtagtcgt cgccaaccaa cacgttgacc tgatcgacgg
      181 gttcgagcag caggatctgc ccggtcttcg cggcttcttt catggcgagc gcgcccgcca
      241 tctgaaacgc catgtcagac gagtcgaccg agtgcgcctt accgtcggtc aaggtgacgc
      301 gcacgtcgac gagggggtag ccggccgcca ctcctcgctc catctgggcg cgcacgccct
      361 tctcgacgga cggaatgaac tgtcgaggca ccgagccacc gaagaccttg tcgatgaact
      421 cgaacccagt gccctggggg agcggctcga tctcgatatc gcagacgccg aactgcccat
      481 gaccgcctga ctgcttcacg tggcggccgt gcgcaaccac cttcgagccg aacgtctcgc
      541 gcatcggtac ccgaagtggc acctgctcga cctcgacgcc gtacttgttc ttcagcctgt
      601 cgagcagcac ttcggcatgt gcttcgccga tgcaccacag gaccagctgg tgggtctccg
      661 cgttcatctc caatcgcaac gtcggatcct cggcggcgag tcgaccgagt ccttgcgaaa
      721 gcttgtcttc gtccgacttc gacttcgcgc gtaccgcgat cggcaacagc ggctccggca
      781 tgtcccacgg gctcaggagc agcgcgtttt ccttgtccga cagggtgtcg gaggtttctg
      841 cacgagcgag cttgccgatc gcacagatgt caccggcgat gcactgcgtg actgggcggt
      901 gtgtcttgcc caggggcgag ctcagggtgc cgacgcgttc gtcgacgtca tggtcctcgt
      961 gaccgcgatc cgccatgccg tgaccggaca cgtgcacgac ggtgtcgggg cgcagcgtcc
     1021 cggagaacac gcgcaccaag gacactcgac cgacataggg atccgtcgtc gtcttcacca
     1081 cttcggcggc cagtggcccg gcggggtcgc aggtcagcgt ggtgatcttc gaaccgtaga
     1141 gcggcatgac tggcggcagc gggtgctcaa gtggcgatgg gaatgcgtgg gcgagcaatt
     1201 cgagaagttc ggcgctgccg atgccggttg ttccgcacac gccgagtacg gggtagaagg
     1261 acccgcgggc gacggccttt tcgaggtcct cgatcacgat tttcacgtcg atgtcctcgc
     1321 ctgcgaggta acgatccatc aacgtctcgt cttcgctttc ggcgatgatt ccttcaagca
     1381 cggcgtcgcg ggtaccgccg atggcctcga gctcggccgc atccgcaggc cgcacttccc
     1441 gccgtccgct gctgtagtca ccgacttgac ggctgagcaa cccgagcaat cccgtcatcg
     1501 tgccgtcggc tgcctggacc ggcacgtaca gcggcaggac gccattgccg aaggtcgcct
     1561 gacacgcgcc gaccaccgcc tcgaaatcgg ctcgcgggga gtcgagtttg gtgatgacga
     1621 ctgcacgcgg catgcccacc tgtgcgcact cttcccacag catccgcgtc gagccgtcaa
     1681 tgccatcaac cgccgacatc acaaacaggg cggcatcggc cgcccgcagc ccggcacgca
     1741 ggtcaccgac gaaatcggcg taccccggcg cgtcaagaag gttgatcttc acgccgtcgt
     1801 gttccaccgg cgccaacgcc agtgagaccg atctctgatg gcgcacctcg gcgtcctcga
     1861 agtcggtcgt cgtggtgccg tcctcgaccc gaccggctcg tgggatcgcg ccggtcgcga
     1921 cgagaagggc ctcgacgagc gtggtcttcc cggctccgga atggccgacc agcacgacgt
     1981 tgcggatctt gcttggctgg tcggccgtcg gtgctgtgcc ggcggctcct gcgggaccag
     2041 tcgccttgtc tgacatgggt cgcgcctcct cagctttgag gtttagaagt cgactctcta
     2101 cctgcgcatt gcgcgccaca agaggttggc cgggtgagca tgtgccgggc ccccgccccg
     2161 gcgaccatgc ctcgcccagt gcagggcggc gctcgggcgt ggcgggcggt cgggtgcccg
     2221 ctgctcgcta cgatggccgg gtcagcccag cacctctcgg tggacggcga aacggacgga
     2281 catgctcaag cgtgcgcgga ttccgctgca acgcgtgctt ggtccgctcg gtcaggcgct
     2341 ggcgcgcgct ggtgtcagcc cgaacgtcgt caccgcactc ggtgcgctcg gagtgtgcgc
     2401 ctcggcactg atctgctacc cgcgcggttg gcttttctgg ggatccttcg ccgtcacgat
     2461 cttcgtgctc tccgatcttg tcgacggcgc attggcgcgg gcccgaggca tcaccgggcc
     2521 gtggggcgcc tttcttgatt cgagcctcga tcgggtcgcc gactccgcgg tgttcgccgg
     2581 tttgatctgg tggttcgccc gcgaaggcaa gccgtggctg ctcggtctgt gcatcttctg
     2641 cctggtggcc ggcaacctgg tgtcttacac gcgcgcgcgc gccgagggtc tcggccttcg
     2701 cgccgacgtg ggcgtcgccg agcgcgccgg tcgcacgatc gtcatcctgg tcgcgaccgg
     2761 tgtgtccgga cttggcgttc cgttcatcca ggccgtcggg ctgtggctgc tggcggtggc
     2821 aagtgcgatc accgtcggtc agcgcttcgc tgtcgtctac cggcaagcga ccagctccgt
     2881 cacccctgca tccatcaccc ccgcgcagga ttcgtgaccg acgcgcgggt ttctggccgc
     2941 gatcggtggc agacacgtgg ctttgtcgcc ggtgcaacgc tggtgcgcgt actgcccgaa
     3001 cgcgtgctcc ggccggtctt tcggctcatc tgcgacgcga tctggcgacg ccacgggcgc
     3061 agtgtccagc aactcgaacg caacctgcgg cgggtggtcg gcgatgccgt gccgacgtct
     3121 gagttggcgg tgctgactcg gcgcgcgatg cgttcgtacg gccggtactg gctggagttc
     3181 ttccggcttc cggtcttttc ccgcgcgcgc attctggacc ggatgatctt cgagggtgag
     3241 gagttcctgt ccggggcgct ggcacaaggg cggggggtgg tgttggccct gccgcactcc
     3301 gggaactggg accacgcggg ggcatggctg gtcctgcgcg gatatccgtt cacgacggtc
     3361 gctgagcggc tgaagccgga agagttgttc gaccggttcg tcgcagcgcg cgagaaggtc
     3421 ggtatggagg tattgccgct gactggggga ggccgcacga ctttcgccct gctgctcgcc
     3481 cggctgcggg ccggcaagac gctgtgtttg gtggccgagc gggagttcgg cgatggcggc
     3541 atcgaggtgt cgttcttcgg tgagccggcg atgatgcccg ccgggccggc atccctcgcg
     3601 ctggccaccg gcgcggcgct gctcccggcg tacctgtggt acagcgacga gggcggaccc
     3661 ggcggcacct ggtgcggctg gatgcgcgag gagataaagc cgcccgccga gggtgacaag
     3721 cgggccaaga ttacggcgat gacacaagcc ctggccgatc gattcgccga agggatcgct
     3781 gcgcacccgc aggactggca catgctccag ccgatgtggc tggccgacgt gcaggcccgc
     3841 gggtgaaggt cggcctggtc tgtccttaca cctgggacgt tcctgggggc gtgca
//
LOCUS       DATCBJ010000399         2706 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_149728, whole genome shotgun sequence.
ACCESSION   DATCBJ010000399 DATCBJ010000000
VERSION     DATCBJ010000399.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2706)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2706)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2706
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_149728"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(200..1063)
                     /locus_tag="VII50_08000"
     CDS             complement(200..1063)
                     /locus_tag="VII50_08000"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HEY5050831.1"
                     /translation="MSPLSAVPAVRRAAVLGSPIAHSLSPVLHAAAYQVLGLRWSYSA
                     IECDEAQLPGVLMSLDESFVGLSLTMPLKRAILPLLDDVSELAVAVGAANTVVFDGLG
                     PFLRRRGENTDVPGIVAAITARRPDGVRNAVILGAGGTAAAALAALRELGVDGTVVVV
                     RDQARAGELRRCADRLGVTPTLVDWPGRAALGEADVIISTVPAGATDQLATQSPAAHG
                     QLFFDVLYDPWPTAFAVASLAAGAAVIGGLELLVQQAARQVELWTGRPAPIEEMRAAG
                     EAAMASRSESS"
     gene            complement(1102..2256)
                     /gene="mltG"
                     /locus_tag="VII50_08005"
     CDS             complement(1102..2256)
                     /gene="mltG"
                     /locus_tag="VII50_08005"
                     /inference="COORDINATES: protein motif:HMM:TIGR00247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HEY5050832.1"
                     /translation="MSSPMDPFDLGLRHSEPKPALYRRLLQVPFAIIASGLVVIAIIV
                     GAVNVTGKVFHPQASNDYTGDGYGSVQVSVHPGQALSAIGETLVEAGVVKSVTAFSDA
                     TNDNAAARSIQPGTYQLRLQMSAASAVALMVSPDSLVGGRVTIPEGLRISRVEALVTQ
                     SDATIKVADIQAALARPTDFGLPAYANGQPEGFLYPATYNVDNTTTATDLLIQMVGRF
                     NQVATSINLAQGAAALKLTPYQVVIIASLIEAEVKRPQDYPQVAEVILNRMHKGMPLE
                     LDSTVNYALGTTKPFLSPTDLKTPSPFNTYLNKGLPPTPIDSPGQDALLAALNPAQGN
                     YIYFVTTDPTTGDTTFTASLTEANALRVLAQKNAAAIAAGSAPASAPASP"
     gene            complement(2253..>2706)
                     /gene="ruvX"
                     /locus_tag="VII50_08010"
     CDS             complement(2253..>2706)
                     /gene="ruvX"
                     /locus_tag="VII50_08010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HEY5050833.1"
                     /translation="GIDVGSVRVGVAASDPGAVLAAPVETVRRSDDRAATRGDLRRIA
                     ALVVERAAIEVVVGLPLSMNGTRGSAAQAALAYAEAIAAAVAPVPVRVVDERLSTVTA
                     HGQLGSAGVRSRARREVVDQAAAVIILQSALDAERSSGRAPGELVTAP"
BASE COUNT          458 a          921 c          910 g          417 t
ORIGIN      
        1 agacgactgc ggcagcttcg acaccagggg tctggtgcaa caactcgcgc agaattcgat
       61 ccagccgact agcccgactc acgtactcga gcggctgctg catagaggac atggagcacc
      121 ttctctcagg acccgtctga cccgggattg tacgggtctt ccggtaaccc atcggcggtc
      181 cggggggtgg gcttgaacct caggagctct ccgaccgcga tgccatcgcg gcctccccgg
      241 ccgcccgcat ctcctcgatc ggggcgggcc gaccggtcca gagttcgact tggcgggccg
      301 cctgctgcac cagaagttca agaccgccga tcaccgcagc ccctgcggcc agcgaggcca
      361 ccgcaaacgc agtcggccac ggatcgtaaa ggacgtcgaa gaacagctgc ccgtgggcgg
      421 ccggcgactg cgtcgcgagc tgatccgtgg ccccggcagg cacggtcgag atgatcacgt
      481 cggcctcccc tagcgccgcc cggcctggcc agtcgaccag cgtgggagtc accccaaggc
      541 ggtctgcgca gcgtcggagt tcgccggcgc gagcctggtc tcgaacgacg acgacagtgc
      601 cgtcgacgcc gagctcgcgg agcgctgcca gggcggcggc ggcggtgcct ccggcgccga
      661 ggatgaccgc gttgcgcacg ccgtccgggc gccgggcggt gatggccgcc acgatgcccg
      721 gaacatcggt gttctcgccg cgcctgcgca ggaacggccc aagcccgtcg aaaactacgg
      781 tgtttgcggc accgaccgcg acggccagct cgctgacgtc gtcgagcaac ggcaggatcg
      841 cccgcttcag cggcatcgtg agcgagagtc cgacaaaact ctcatcaagg ctcatcagga
      901 cacccggcag ctgcgcctcg tcgcactcga tcgccgagta gctccagcgc agcccaagca
      961 cctggtaggc ggccgcgtgc agaaccggcg acagggaatg ggcgatgggc gagcccagca
     1021 cagccgcgcg gcgcacggca gggacggcgc tcagcgggct caccagcgga cgctcaaggt
     1081 cacggactcc accacgccgc tttacggtga ggccggcgcc gacgcaggcg ctgatcctgc
     1141 cgcgatggcc gccgcattct tctgtgcgag cacccgaagc gcattcgcct ccgtcaacga
     1201 cgccgtgaag gtcgtgtcac ccgtcgtcgg gtctgtcgtc acgaagtaga tgtagttacc
     1261 ctgcgcggga ttcagcgcag cgagcagcgc atcttgcccc ggggagtcga tcggcgtcgg
     1321 tggcaaaccc ttgttcaggt aggtgttgaa gggcgacggg gtcttgaggt cggtcgggct
     1381 gaggaacggc ttggtggtgc ccagcgcgta gttcacggtc gagtcgagct caaggggcat
     1441 ccccttgtgc atccggttga ggatgacctc ggcgacctgc ggatagtcct gcggccgctt
     1501 cacctccgcc tcgatcaggc tggcgatgat cacgacctga tagggcgtca gcttcagggc
     1561 ggctgcgcct tgagcgaggt tgatcgacgt ggcgacctgg ttgaatcgtc cgaccatctg
     1621 aatcagtaaa tccgtcgctg tcgtcgtgtt gtcgacgttg taggtggccg gatagaggaa
     1681 gccttccggc tggccgttcg cgtacgcagg caggccgaaa tcggtcggcc tggccaaggc
     1741 ggcctggata tcggcaacct tgatcgtggc atcgctttgg gtgaccagcg cctcgacccg
     1801 gctgatgcgc aggccttcag gaatggtgac ccggccgccg accagcgaat ctggcgacac
     1861 catcaaggcg accgcgctgg ccgcggacat ctgcaggcga agctggtagg tgccgggctg
     1921 gatcgagcgc gctgccgcgt tgtcattggt cgcatcgctg aaggcggtca cgctcttgac
     1981 gacgccggcc tcgacaaggg tttcgccgat cgccgacagc gcctgacccg ggtgcacgga
     2041 gacctgaact gagccatacc cgtcgcctgt gtagtcgttg gaggcctgcg ggtggaacac
     2101 tttgccggtc acgttgaccg caccgacgat gatcgcgatg acgaccaggc cagaggcaat
     2161 gatcgcgaac ggcacctgaa ggagccggcg gtagagcgcc ggctttggct cgctgtggcg
     2221 caggccgagg tcgaacgggt ccatcggcga actcacggcg cggtcaccaa ctcccctggt
     2281 gcccggcccg acgatcgctc ggcgtcaagg gcactctgca agataatcac agctgccgct
     2341 tggtcgacga cctcccgacg cgctcgcgag cgcacaccag ccgaaccaag ctgcccgtgc
     2401 gcggtgacgg tcgacaaccg ctcgtcaacg acccggaccg gtacgggcgc caccgcggca
     2461 gcgatcgcct ccgcatacgc gagagctgcc tgcgccgcag acccgcgggt cccgttcatc
     2521 gacagcggca ggccgaccac cacctcgatc gcagcgcgct cgaccaccag cgcagcgatt
     2581 cggcgcagat cgccgcgtgt ggcagcccgg tcatcactgc gccgcactgt ttcgaccggc
     2641 gccgctagaa ccgcgcctgg atcgctcgcc gctactccga cgcggacgct gcccacgtca
     2701 atcccg
//
LOCUS       DATCBJ010000400         2486 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_30441, whole genome shotgun sequence.
ACCESSION   DATCBJ010000400 DATCBJ010000000
VERSION     DATCBJ010000400.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2486)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2486)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2486
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_30441"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(87..632)
                     /locus_tag="VII50_08015"
     CDS             complement(87..632)
                     /locus_tag="VII50_08015"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SigE family RNA polymerase sigma factor"
                     /protein_id="HEY5050834.1"
                     /translation="MEPNGGVGCLDGMYSLTQDASAPRLDARVKSGLDGDSLEDLYRR
                     CRPGLVRLAHLLTGSATAAEDVVHDAFIGFARSKSQVSDPPAYLRRSVVNLSTNIHRR
                     TARERRHLASVTESVVDPAEVDETWQLVRQLPPRQRAVLVLRFYLDLSETETARLLGC
                     KPGTVKSSTARALAKLKERMS"
     gene            complement(666..1751)
                     /locus_tag="VII50_08020"
     CDS             complement(666..1751)
                     /locus_tag="VII50_08020"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HEY5050835.1"
                     /translation="MTIEFNRTDPTEPVPAAQRDELLTNPGFGRVFTDHMVTITWTPD
                     AGWHHASLRPYGPLALDPATAVFHYGQSIFEGFKAYVQPDGSIKTFRPDANGRRFQRS
                     AARLALPELPVEDFVAAADLLIEADRDWVSPNDEKSLYIRPFMMATEAFLGVRPANHV
                     TFLIIASPAGAYFAGGLHPVSIWLSEEYTRAAPGGTGAAKCAGNYAASLVAQQEASAN
                     GCDQVVFLDAVEKRWVEELGGMNVYFVFDDGTLVTPPVSGTILEGVTRDAILTIAAEH
                     GHATQQRPIDIDEWRSGVTSGRITEVFACGTAAVVTPLGRLAWRGGELTMGDGQPGKV
                     TQDLRDSLLAIQYGTAPDPHGWMHTVC"
     gene            complement(1945..>2486)
                     /locus_tag="VII50_08025"
     CDS             complement(1945..>2486)
                     /locus_tag="VII50_08025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="isocitrate/isopropylmalate family dehydrogenase"
                     /protein_id="HEY5050836.1"
                     /translation="TLVHKNNVLVHSGDLWTRTVKAVSADFPDIAVDYCHVDAATIYF
                     VTDPQRFDVIVTDNLFGDILTDLGAAIAGGIGLAASGNINPARTTPSMFEPVHGSAPD
                     IAGKAIADPTATVLSVAMLLDHLGLPEAAATVEAAVAADLAAIAPHRAATSNSFRVPE
                     PVRRATHEVGDALAARVAG"
BASE COUNT          388 a          813 c          871 g          414 t
ORIGIN      
        1 atcgaagcga ccgccgctga tcggcgtccg gtcggccatc gtgcgaaggg cagcccgcaa
       61 gcgggccagt gcctcgtcgt cgggggtcat gacatcctct ccttgagctt cgcaagcgcg
      121 cgggcggtcg acgatttgac ggtgccgggc ttgcacccga gcaaccgcgc cgtctccgtt
      181 tcggaaaggt cgaggtaaaa ccgcagcacc agcactgcac gctggcgcgg cggcagttgc
      241 ctgacgagct gccacgtctc gtcgacctcc gcggggtcga caaccgactc ggtcacgctg
      301 gcgaggtgcc gacgttcgcg ggccgtgcgg cgatggatgt tcgtgctcag gttcacgacc
      361 gagcgccgca gatacgcagg cgggtcgctc acctgcgact tgctgcgggc gaacccgatg
      421 aaggcgtcgt ggacgacgtc ttcggccgcc gttgccgacc cggtcagcag gtgcgcgagt
      481 cgcaccagcc caggacggca gcgacggtac aggtcctcga ggctgtcgcc gtccaggcca
      541 cttttcaccc gagcgtcgag ccgtggcgca ctcgcgtcct gcgtcaggct gtacatgcca
      601 tcaagacacc ccaccccgcc gttcggttcc atcgcaccgg ccccacgtgc cacggccgtc
      661 ggaattcagc agacggtgtg catccatccg tgcgggtcgg gggcggttcc gtattggatg
      721 gcgagcagcg aatcgcgcag gtcctgggtg accttgccgg gttggccgtc gcccatcgtg
      781 agctctcccc cgcgccaagc caaccggccg agcggcgtca cgacggcggc cgtaccgcag
      841 gcgaagacct cggtgatccg acccgatgtc acgcccgagc gccactcgtc gatgtcgatc
      901 ggacgttgct gcgtcgcgtg cccgtgctca gccgcgatcg tcaggatcgc gtcgcgggtc
      961 acgccctcga ggatcgtgcc ggacaccggc ggcgtcacca aagttccgtc gtcaaacacg
     1021 aagtacacgt tcatgccgcc gagttcttcg acccagcgct tctcgaccgc gtcgaggaag
     1081 acgacctggt cgcacccgtt cgcgctcgcc tcttgctgtg cgaccaggct ggccgcgtag
     1141 ttgcccgcgc acttcgccgc gccggtacca ccgggtgccg cgcgggtgta ctcctccgac
     1201 agccagatcg acaccggatg caagccgccg gcgaaatagg cgccggcggg cgaggcgatg
     1261 atcaggaacg tcacgtgatt cgccggccgg acgccgagga aagcctccgt cgccatcatg
     1321 aaaggcctga tgtacaagct cttctcgtca ttgggcgaca cccagtcgcg atcggcctcg
     1381 atcagcaggt ccgcggccgc cacgaagtcc tcgacgggca gctcaggcaa cgcaagccgc
     1441 gccgcggacc gctggaaccg gcgtccgttc gcgtctgggc ggaaagtctt gatcgagccg
     1501 tccggttgca cgtaggcctt gaagccctcg aagatcgact gtccgtagtg gaaaacggcg
     1561 gtcgccgggt cgagcgcgag cggaccgtaa ggccgcaggc tcgcgtggtg ccaaccggcg
     1621 tccggcgtcc aggtgatcgt gaccatgtgg tcggtgaaga cccggccgaa gccgggattc
     1681 gttagtagct cgtcacgttg agcggccggc accggctcgg ttgggtcggt cctgttgaat
     1741 tcgatggtca ttggtcaatc ctcacggcta atgtggttgc gacgctagcc caccgcgtcg
     1801 cacgtgggct caggtgaccc gctggcctcg gtgattcatg aggctgggtc gcgaagggca
     1861 aagggccgga cgccggtcag ctggcgttgc ccggttcgtc gacatccgcc atggcatggg
     1921 gttcgcggtg cccgggcgcc gggttcagcc ggcgacgcga gcggccagtg cgtcgccgac
     1981 ctcgtgggtc gcgcggcgga ccggctcagg tactcggaac gagttcgacg tagcggctcg
     2041 gtgaggagcg atggctgcga ggtctgcggc gactgcggct tcgacggtgg cggccgcttc
     2101 ggggagtccg aggtggtcga gcagcatcgc gacggacagc acggtggccg tcgggtcggc
     2161 gatcgccttg ccggcaatgt cgggcgcgct gccgtgcacg ggctcgaaca tgctcggcgt
     2221 ggtccgggcc ggattgatgt tgccgctggc cgcgagtccg ataccgccgg cgatcgccgc
     2281 accaaggtcg gtgaggatgt cgccgaacag gttgtcggtc acgatgacgt cgaagcgctg
     2341 cgggtcggtc acgaagtaga tggtcgcggc atcaacgtgg cagtagtcga ccgcaatgtc
     2401 ggggaagtcg gcagagaccg ccttgaccgt tcgggtccag aggtcgcccg agtgcacgag
     2461 cacgttgttc ttatgcacca acgtca
//
LOCUS       DATCBJ010000401         3618 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_124719, whole genome shotgun sequence.
ACCESSION   DATCBJ010000401 DATCBJ010000000
VERSION     DATCBJ010000401.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 3618)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3618)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3618
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_124719"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..276)
                     /locus_tag="VII50_08030"
     CDS             complement(<1..276)
                     /locus_tag="VII50_08030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5050837.1"
                     /translation="MALLGPPHIEYDGQPVHFDTRKAIALVALVSMSEQPLSRDRLAL
                     LLWPDSDDERARGALRRTVSVTAAGVGDALVVSRTTIALDERKVRVDA"
     gene            559..1851
                     /locus_tag="VII50_08035"
     CDS             559..1851
                     /locus_tag="VII50_08035"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HEY5050838.1"
                     /translation="MAESLLSHHAAPATTTEAAGATTPGAAGAHTGVTVTAAPDLIPD
                     QDARRSRLARLRRRAGATTLPESAAPVHAGPSFEIAQNDPILGYCQSADGPVDITDLV
                     LDSPGLAAMRAAGVVLVVPLVSSGELIGLLHLGPRLSERDYSTDDRHLLDSLAGHAAP
                     AIRVGQLVLEQQAEARQRERIEQELKVAQLIQQQFLPKELPDLPSWTVAAFYRPARQV
                     GGDFYDFISLRDGRVMVVVGDVTDKGVPAALVMASTHSLLRSTAPELTSPGAVLAHVN
                     DLLCHDMPSAMFVTCLALLLDPKSGEIEYANAGHDLPFLRTADGVVELKARGMPLGLM
                     TGMDYEQKTAELQTGDCLLLHSDGLAEAHSPTREMFGFPRVGEVVGRGLSGEALIDLC
                     LSELDRFTGPDHEQEDDITLVSLQRTGGSRFVDLEDRS"
     gene            1848..2339
                     /locus_tag="VII50_08040"
     CDS             1848..2339
                     /locus_tag="VII50_08040"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF024970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY5050839.1"
                     /translation="MTSNRAEETPQLLATFAVASEPGNERVALREVAAAAADCGLSPD
                     QMERLKTAVAEATMNAIEHGNENRAEIPVDIAVMRTLDAVAVTITDRGGDRADHVERE
                     VPDIELKLRGEQRPRGWGLFLLQNMVDDMEVSTHGTLHTVRLTMRTGGEDGNEGDQHA
                     EQL"
     gene            2323..2673
                     /locus_tag="VII50_08045"
     CDS             2323..2673
                     /locus_tag="VII50_08045"
                     /inference="COORDINATES: protein motif:HMM:NF013868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HEY5050840.1"
                     /translation="MPSNCEVDVRRLPGTVVLVMRGEIDASASQPLQSAYAGTLGPDM
                     ATRVVLDFTAVDYMNSTGIALIVGILAQARAEHRTVAASGLSEHYRQIFEITRLSDFI
                     QMFADVDDAIAITT"
     gene            2713..3072
                     /locus_tag="VII50_08050"
     CDS             2713..3072
                     /locus_tag="VII50_08050"
                     /inference="COORDINATES: protein motif:HMM:NF013868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HEY5050841.1"
                     /translation="MDAPAVSMDVRHIAEGTAVVDIAGEVTAACEPILMSAYQQVSDN
                     DTRRVVLNFSGLEYMNSGGIGMLVTLLVRTNRQHQQLSAYGLSPHYRQIFELTRLDEA
                     ITIFDDETAALAALTTR"
     gene            3083..>3618
                     /locus_tag="VII50_08055"
     CDS             3083..>3618
                     /locus_tag="VII50_08055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5050842.1"
                     /translation="MNTQRPDVKSADDGGTPAETTPVASALAPRRDQQNWAANVQRLS
                     VEKRDGVRGTNVAGRRLTGPIQGFGKMWQKTYVTSLGRDVTPEHAISEWRTHFADFWP
                     KGNRFAGGLAGLNPGDVALIDMSLAPGAPRLSTGVMVLYADEQSFTFMTPQGHMFAGW
                     ITFSAERTATGTTNLQAQV"
BASE COUNT          662 a         1218 c         1161 g          577 t
ORIGIN      
        1 ggcgtccacc cgcaccttgc gttcgtccag tgcgatggtc gtgcggctga cgacgagcgc
       61 gtcgccgaca cccgccgcag tcaccgatac cgtgcgacgc aacgcgcccc tagcccgctc
      121 gtcgtcggag tctggccaca acaatagggc gagtcgatcc ctactgagtg gctgctccga
      181 catcgagacg agcgctacaa gcgcgatcgc cttccgcgtg tcgaaatgga ccggttggcc
      241 gtcgtactcg atatgcggcg gccccagaag ggccacgttc agcacctggc ggcctccaag
      301 ggtcgactgc ggacacgact gtgacggcag atggaaatct cgcgatgacc gtaacgcttt
      361 cggaacgctg ggccgccaaa gtagtcctag acgggccgac aagacagcgg tccggcgacc
      421 gaggagccgg cgagccgggg aggaattcat gcagccgatc tgcgcagatg tgccacctgc
      481 ggtcctacgc tggtcgcctt ccacgcctgc ctttaccggc tggctccgtt ccatctgcaa
      541 gcacagagtg gggtcatgat ggccgagtcc ctgctgtcgc atcacgccgc acccgcgacc
      601 acaaccgaag ccgccggcgc gaccacaccc ggcgccgcgg gcgcccatac cggggtgacc
      661 gtcactgccg cgcccgacct gattccagat caggacgccc gtcgttcgag gctggcccgg
      721 ctgcgccgac gcgccggagc aacgacgctc cccgaatcag ccgcgccggt ccacgccggt
      781 ccgtcgttcg agatcgccca gaacgatccg atcctcggct actgccagag cgcggacggc
      841 ccggtcgaca tcaccgacct tgttctggac tcccccggcc tcgctgccat gcgggcggcc
      901 ggcgtcgtcc tcgtcgtccc gcttgtgtcc tccggcgaac tgatcggcct gcttcatctg
      961 gggccccggc tttccgagcg tgactactcc accgacgacc ggcacctcct cgactccctc
     1021 gctgggcacg cagcgcccgc gatccgtgtc ggccagctgg tcctggagca gcaggccgag
     1081 gctcgccaac gcgagcgcat cgagcaggaa ctcaaggtcg cgcagctcat ccagcagcag
     1141 tttctgccca aggaactgcc ggacctgccg agctggaccg tggccgcgtt ctatcgtccc
     1201 gcgcggcagg tgggcgggga cttctatgac ttcatctcac tgcgcgacgg ccgggtcatg
     1261 gtcgtggtgg gggacgtcac ggacaagggt gtcccggcgg cgctggtcat ggcgagcacc
     1321 cactcgctgc ttcgatcaac tgcgcccgaa ctgacatcac cgggtgcggt cttggcccac
     1381 gtcaatgacc tgctctgcca cgacatgcca tcggcgatgt tcgtcacgtg cctggcgttg
     1441 ctgctcgatc cgaagagcgg cgagatcgag tacgccaacg ccggccacga cctgcccttc
     1501 ttgcggaccg cggacggtgt ggtcgagctc aaggcccggg gcatgccgct cggcctgatg
     1561 accggcatgg actacgagca gaagaccgcc gagctgcaga ccggcgattg tctgttgctc
     1621 cacagcgacg gtctcgcaga ggcgcacagt cctacccgcg agatgttcgg cttcccccgc
     1681 gtcggcgaag tcgtgggccg cgggctgtcc ggcgaggcgc tcatcgacct gtgcctgtcc
     1741 gagctggacc ggttcaccgg gcccgatcac gagcaggagg acgacatcac gttggtcagt
     1801 ctgcagcgca ccggtggatc acggttcgtc gacctcgagg accgatcatg accagcaacc
     1861 gagcggagga gacgccacag ctacttgcga cattcgctgt ggcgagcgag cccggaaacg
     1921 agcgcgtagc gctgcgcgaa gtcgccgccg cagccgcgga ctgcggtttg tcgcccgatc
     1981 aaatggaacg gctcaagacc gcagtcgccg aggcaacgat gaacgcgatc gagcacggca
     2041 acgagaaccg cgcggagatc ccggttgaca tcgccgtcat gcggacgctc gacgccgtcg
     2101 cggtcacgat caccgatcgg ggcggggacc gtgccgacca cgtcgagcgt gaggtacccg
     2161 acatcgaact gaagctgcgc ggggagcagc ggccacgcgg ttggggcttg ttcctcctgc
     2221 agaacatggt cgatgacatg gaggtgtcga cccacggcac cttgcatacc gtgcggctga
     2281 cgatgcgcac gggtggagaa gacggcaacg aaggagacca gcatgccgag caactgtgag
     2341 gtagacgttc gacggcttcc aggcaccgtc gtcttggtga tgcgcgggga gatcgatgca
     2401 agcgcatcgc aacccctgca gtccgcctac gcggggacgc tgggacccga catggcaacg
     2461 cgcgttgtac tcgacttcac cgcagtcgac tacatgaact ccaccggcat cgcgttgatc
     2521 gtgggcatcc tcgcgcaagc ccgcgcggag catcgcacgg tagccgcgag cgggttgtcc
     2581 gagcactatc gccagatctt cgagatcacc cggttgtccg acttcatcca aatgttcgca
     2641 gacgtcgacg acgccatcgc catcaccact tagtcaacac acttgacggt ctccgtcgag
     2701 aggggaaaca ccatggacgc accagccgtg agcatggacg tacgtcacat agcggagggt
     2761 acagcggtag tcgacatcgc cggcgaggtg actgccgcgt gcgagccgat cctgatgtcg
     2821 gcgtaccagc aggtgagtga caacgacacg cgacgcgtcg tcctcaactt cagcgggctc
     2881 gagtacatga acagcggcgg catcgggatg ctcgtgacgt tgctggtgcg cacgaaccgg
     2941 cagcatcagc aactctcggc gtacgggttg tcgccgcact accggcagat cttcgagctg
     3001 acccggctcg acgaagctat caccatcttc gacgacgaaa cggctgcact tgcagccctc
     3061 accaccaggt gaagaggaag ccatgaacac gcaacgacca gacgtcaagt ctgccgacga
     3121 cggcggcacg cccgccgaaa caactccagt cgcgagtgcg cttgctccgc ggcgcgacca
     3181 gcagaactgg gcggccaacg tgcagcgcct cagtgtcgag aagcgcgacg gtgtccgcgg
     3241 gaccaatgtc gccggccgcc ggctgaccgg accgatccag ggcttcggca agatgtggca
     3301 gaagacctac gtcacgtctc ttggccgtga cgtaacacca gagcatgcga tcagcgagtg
     3361 gcgtacgcat ttcgccgact tctggccgaa gggaaaccgc ttcgccgggg gcttggccgg
     3421 cttgaaccct ggcgacgtgg cattgatcga catgtcactc gcccccggtg cgcctcggct
     3481 ctcgaccggc gtgatggtgc tgtacgccga tgagcagtcg tttacgttca tgacccctca
     3541 aggtcacatg ttcgccggtt ggatcacctt cagcgcggag cggacggcga cgggcacgac
     3601 gaacctgcag gcccaggt
//
LOCUS       DATCBJ010000402         2561 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_78712, whole genome shotgun sequence.
ACCESSION   DATCBJ010000402 DATCBJ010000000
VERSION     DATCBJ010000402.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2561)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2561)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2561
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_78712"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..465)
                     /locus_tag="VII50_08060"
     CDS             complement(<1..465)
                     /locus_tag="VII50_08060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017978889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HEY5050843.1"
                     /translation="MQFTTESSAAFQEIVAPWVHPSMEYKLLPRFRGLFHLEPEFVSP
                     SERPVPARILDIHVKPKTRSTHRFDIEVEGNHNYFVDGVMVHNSPETTTGGKALKFYA
                     SIRIDVRRIETLKDGQEAVGNRTRAKVVKNKCAAPFRQAEFDIIYNQGISREG"
     gene            complement(445..1314)
                     /locus_tag="VII50_08065"
                     /pseudo
     CDS             complement(445..1314)
                     /locus_tag="VII50_08065"
                     /inference="COORDINATES: protein motif:HMM:NF015141.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1297..1908)
                     /gene="recA"
                     /locus_tag="VII50_08070"
                     /pseudo
     CDS             complement(1297..1908)
                     /gene="recA"
                     /locus_tag="VII50_08070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019513023.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
     gene            complement(2127..>2561)
                     /locus_tag="VII50_08075"
     CDS             complement(2127..>2561)
                     /locus_tag="VII50_08075"
                     /inference="COORDINATES: protein motif:HMM:NF022693.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3046 domain-containing protein"
                     /protein_id="HEY5050844.1"
                     /translation="PEAAADDVALGFGVRRVQPSMAPTRAAAPASRRNSRRSMGFLTD
                     VWARWDESLPGDVWAPGLGARVPLDVRLSCGRHTAAVRLTDFWGRMRQHFGATYAASF
                     AHDFVLSELGGRTIDQALAQGVDVQEIWRAVCRTVEVAPKLR"
BASE COUNT          469 a          843 c          774 g          475 t
ORIGIN      
        1 gccttcgcgg cttatgccct gattatatat gatatcgaac tccgcctggc gaaagggcgc
       61 cgcacactta ttcttcacaa ccttcgcacg agtgcggtta ccgaccgctt cctggccgtc
      121 tttcaaggtc tcaatgcgac gcacgtcgat gcgaattgac gcatagaact tcagcgcctt
      181 tccgccggtc gtagtctccg ggctgttgtg caccatgacg ccgtcgacga agtagttgtg
      241 gttgccctcg acctcgatgt cgaagcgatg cgtcgaccgg gtcttcggct tcacatgaat
      301 gtcgaggatc cgtgcaggca ccggccgctc ggacggcgag acgaactccg gctcgaggtg
      361 gaagagcccc cggaaccgcg gcagcaactt gtattccatc gacgggtgca cccacggtgc
      421 cacgatctct tggaacgcag ctgaggattc ggtcgtgaac tgcatcacct gctgcccgcg
      481 agcaccgcgc gttgtcaact tgacgtcgag cttgtgcgtg tcgcgcaagt actcgacgat
      541 gcgatcgcgg gtgccaacgg tcattgcgtc gagacagatc tcgattcgac cactgccgcc
      601 ctgagtgcgc tcttgcaagc ccctcgaacg cacggtgaaa caaccatcgt ccatgtacca
      661 aatcgcgagt gcgagtggcg tgagagcctt caggtactcc tcggacagat gcttcttacc
      721 gtcaccgaaa tagacggcct gccgaagctc gtcgagctcg ggcaacggag tgaagtcggc
      781 aaataccgcg ccgcctgcgc ttgtccggcg gctgcacccg atgttcgcga atagcgagac
      841 tttccagtcg aggtaatcga cctgcttcgg gccgtgcccg agacgaaagc gcactccggt
      901 gcggtccctt cggttcggcg acaagttgcc gtcgcccatc aacgacccga gcacgacctg
      961 catctgctgt gaactcaacc tgtgcggctc agtgaccatc acccggtcgc cggcgagaag
     1021 ctcaccagcc gggcgccagc cacccggcgt ccggatgagg tggttggcgg tggcggcaaa
     1081 ctgcgcacgc ccgttgcggc ccgacttggc cacggtgaac tggagaaact cgtcagtcgg
     1141 accgttgtcg aaccagttga cgatcttgcg gggcacaatc cggtcggtct caggatcgta
     1201 ggaaagaacc tcgaccgcca tcttttggtt gacgatcttg ccgatcttct cttgcgtgcc
     1261 gtcagcgaga gtgactcggg tgttgtagtg gaagcaccca aagaagacgc cgatcttttc
     1321 gcgcaactgg ttgataaaaa tcgccgttgt gcccgagttg ctcaacgcac cggtgatctt
     1381 gcgcaacgcc tggctcatca accgtgcttg cagaccgacg tgactatcgc ccatctcgcc
     1441 ttcgatctcg gcgcggggca ccagcgcggc gaccgagtcg atcacgatga tgtcgatcgc
     1501 ccctgagcgg atcagggtgt cggcgatctc gagcgcctgc tcgccggtgt cgggctgcga
     1561 caccagcaac gcgtcgctgt cgacgccgag cttggcggcg tactcggggt caagcgcgtg
     1621 ctcagcatcg atgaaggccg cgatgccacc ggcccgctgg gcgttggcga ccgcgtgcaa
     1681 cgcgagcgtg gtcttgccgc tggactcggg gccgtagatc tccacgatcc ggccgcgcgg
     1741 caacccaccg atgccgagtg cgacgtcaag agcgatcgaa ccggtcggga tcacttcgat
     1801 cggttcccgc acctcatcac ctaggcgcat caccgagccc ttgccgtgcg cgcgctcgat
     1861 ctgggcaagc gcgttctcaa gggctttcgc gcggtcgggt gctgccatgg tcgccatccg
     1921 ttcagtcgcc gtgtgcggtc gcaagtctct gggtcgtgga cgacgttagg cgccagggat
     1981 gccactttcc tgtcaccgga cggccgctat ggacgactcc gatagctgtg gacattaacc
     2041 gaacacctgt tcgaacgacc ggcgacacgc cgaccggaac cgaccaccgg acctcggcta
     2101 atcccaccag tcccagacgc cacagatcag cgcagtttgg gcgcgacttc gactgttcgg
     2161 cagaccgctc gccagatctc ctggacgtcg acgccctggg ccaacgcctg gtcgatcgtg
     2221 cgcccgccga gctcgctcag cacgaaatcg tgagcgaacg atgcggcgta ggtcgcgccg
     2281 aagtgctgcc gcatccggcc ccagaagtct gtcaatcgca cggcggcagt atgcctccca
     2341 cacgacagcc ggacgtcgag tggcacgcgt gcacccaatc caggcgccca gacgtcccca
     2401 ggtagagact cgtcccatcg agcccacaca tctgtgagga atcccatcga ccggcgcgag
     2461 ttccttcgac tagctggcgc cgccgccctg gtaggtgcca ttgagggctg tacacgccga
     2521 accccaaatc caagcgcgac gtcgtcagca gccgcttccg g
//
LOCUS       DATCBJ010000403         7268 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_197867, whole genome shotgun sequence.
ACCESSION   DATCBJ010000403 DATCBJ010000000
VERSION     DATCBJ010000403.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 7268)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7268)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7268
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_197867"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..545)
                     /locus_tag="VII50_08080"
     CDS             complement(<1..545)
                     /locus_tag="VII50_08080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEY5050845.1"
                     /translation="MRRLTAVPLGALALGSAAIGYASGIERRSPRLRRFEVPILPPGS
                     SPLRVLHFSDAHLTPGQTWKRDWIHSLAALEPDLVINTGDSIAHVGAVPVFLDAIGPL
                     LERPGAFVFGSNDYAAPVFKNPARYLFPDDGSRPKGVELPWRALREAMLDAGWLDLSN
                     SRGYVKADGRQVELVGVDDPH"
     gene            587..1048
                     /locus_tag="VII50_08085"
     CDS             587..1048
                     /locus_tag="VII50_08085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009075836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HEY5050846.1"
                     /translation="MSTAPLKTRLHDDLTTSIKARDELRTATLRMVLSAITNEEVSGT
                     EARQLSDDEVLKVLTREGKKRREAAEAFAAGNRPELAERERAEGEVIATYLPAQLSDD
                     ELRDLVTQAVASAGASGPQAMGAVMKVVNPLVAGRADGGRVAAEVKRQLAG"
     gene            complement(1074..3473)
                     /locus_tag="VII50_08090"
     CDS             complement(1074..3473)
                     /locus_tag="VII50_08090"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HEY5050847.1"
                     /translation="MLGVLSLLVGLLVAGLALPAAAGLGITTKNAIGDITFADLPASL
                     QSPPLSQLSVINAADGTPLASFYYENRQSVSLDAVAPTMRQAIVAIEDSRFYEHHGID
                     PKGILRALVTDTSSGGAVQGASTITQQYVRQALVETNAQDNNTAAAAAATSKNLTRKL
                     TEARYALALEQKLTKDQILEDYLNTVYFGDGAYGISAAAQHYFGETADQLTLSQSALL
                     AGVINNPSTFDPTVNPKDALARRNVVLDRMLVNYPDSYTSTQIDAAKASPLGLTIVPV
                     PNGCATSSAPFFCDYVVHDILNGPEFGATVTDRANLLRRGGLVVTTTLQPAVQKAAQL
                     AVDTVVPESPALNAGLAAAIDMVNPQTGAIEAMAVDRPYGTGFGQTEVNLAADYVNHG
                     GSQGRNAGSTFKIFVLAAALEEKIPTGTTIKSPAKITIPKGSTTTCDGLPTSVWNVVN
                     AEDSEGGTFNMVQATAQSVNTYFAQLEVKTGLCAPVTIAQDLGVHKATGGDLDQIPAF
                     TLGAEPVDPLSVTGAFAGLANNGTFCTPQSITKVTWQVGDVTTNRPVPGPNCGKVLDP
                     SIAEGVTTLLQGVLGQGGTGYGLALGRPAAGKTGTDDEFKNAWFSGYTPNLASSVWVG
                     NPDSTVPMQAITVNNKKYAYVYGATLPGPIWQLAMKGALLNQPIASFQNPPDTVLRGP
                     AITIPNLQGQDPAAAAAALTKLGLSPAVDPTKVTSSQPLGKVETTIPASGTIVYAGAL
                     VKIELSNGIPPFVSPPASSEPPSSEPPSSQIPGSPSPITITGGTPTPTVTPSPTSTPT
                     H"
     gene            3714..4022
                     /locus_tag="VII50_08095"
     CDS             3714..4022
                     /locus_tag="VII50_08095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019527064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WhiB family transcriptional regulator"
                     /protein_id="HEY5050848.1"
                     /translation="MAWTTDWTTRAGCRNADPDALFVQGAAQNRAKAVCFGCPVRAEC
                     LADALDNRVEFGVWGGLTERERRALLRRRPNVTSWANLLSTAMTDFERDAAEAYSATG
                     "
     gene            complement(4032..5198)
                     /locus_tag="VII50_08100"
     CDS             complement(4032..5198)
                     /locus_tag="VII50_08100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsA family ATPase"
                     /protein_id="HEY5050849.1"
                     /translation="MTHLDIETLIADRGTKIIVCCGSGGVGKTTLAAALGVRAAEQGR
                     RVVTLTIDPARRLAQSLGLQELDNVPRKVAGMKLKKRADGVKNEGELHAMMLDMKRTF
                     DEIVEAHASPAKAAQILANPFYQSLSSSFAGTQEYMAMEKLGQLRASGQWDLIVVDTP
                     PARSALDFLDAPARLARFLDGRFIRMLLVPAKVGGRGYTKVVQVGLGLVTGTMMKLLG
                     TQLLTDVQTFVAAMDTMFGGLRQRADATAKLLRAPGTVFVVVASPEPDALREASYFVD
                     RLVEEDMPLAGVVVNRVHRSTVPQITAVNALSAAEALDGEDDADGDLDGALTAGVLRV
                     HAERLTMQATEQRRVDGFALAHPNVLLASARALAQDVHDLNGLRTLGDALCETA"
     gene            complement(5195..6247)
                     /locus_tag="VII50_08105"
     CDS             complement(5195..6247)
                     /locus_tag="VII50_08105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsA-related P-loop ATPase"
                     /protein_id="HEY5050850.1"
                     /translation="MNAQRGDSGRDRDWDGVRLHIVTGKGGTGKTTVAAALALALATG
                     GRRTLLVECEGRQGLARLFDSPPLPYAERRVAVAPDGGVVYALAIDPEEALLEYLEMY
                     YHLGRAGRALRRLGAIDFATTIAPGVRDVLLTGKVYEATRRRTTHKSTRTSPGDRAFD
                     VTDDFVYDAVVMDAPPTGRIARFLNVNSEVASLARVGPVNRQAESIMRLIRSPQTVVH
                     LVTMLEEMPVQETLDGIEELHEFDLPVGGIFVNRVHEPLLPTSELKAAGAGAIDLTAL
                     ARGLKSAGLPAGDAITAGLSMEAAEHALQLAVERKLRSRLRAAARPTYRLHDLDTTDV
                     GRLYALAAELVEQGAA"
     gene            6278..6436
                     /locus_tag="VII50_08110"
     CDS             6278..6436
                     /locus_tag="VII50_08110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019355547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5050851.1"
                     /translation="MTKWEYATVPLLVHATKQILDNWGSDGWELVSLVPGPNSEQLVA
                     YMKRPVES"
     gene            6433..6948
                     /locus_tag="VII50_08115"
     CDS             6433..6948
                     /locus_tag="VII50_08115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEY5050852.1"
                     /translation="MSTPSLTTPEDRLIAMGLTLPAVSAPLAAYVPALRSGNYVYTSG
                     QLPTVDGELVAVGKVGTGPGASVSPEEANAAARICALNALAALRSVVGELSSVVRIVK
                     VLGFVASAPDFTAQAAVINGASELLLEVFGTAGGAAAPIGSHARSAVGVAVLPRDAPV
                     EVELIAEVTPA"
     gene            7012..>7268
                     /locus_tag="VII50_08120"
     CDS             7012..>7268
                     /locus_tag="VII50_08120"
                     /inference="COORDINATES: protein motif:HMM:NF013015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HEY5050853.1"
                     /translation="MTPGGQPTILHADLDAFYASVEQLLDPSLRGRPIAVGGGVVLAA
                     SYEARAFGVTGGMSGRRARQLCPGLRFVGGHFHEYQRLGDQV"
BASE COUNT         1143 a         2355 c         2510 g         1260 t
ORIGIN      
        1 atatgcggat catcgacgcc gaccaactcg acctgccggc catcagcctt cacgtacccg
       61 cgtgaattcg aaaggtcgag ccaaccggcg tccagcatcg cttctcgaag cgcgcgccac
      121 ggcaactcga cgcccttcgg ccgcgaaccg tcgtccggga agaggtagcg cgccggattc
      181 ttgaacaccg gcgccgcata gtcgttggac ccgaacacga acgcgcctgg tcgttcgagc
      241 agcggcccga tcgcatcgag aaagaccggc actgcgccga cgtgcgcgat gctgtcgccg
      301 gtattgatca ccaggtcggg ctcgagggcg gccaacgagt ggatccaatc gcgcttccag
      361 gtctggcctg gcgtcaggtg cgcgtcggag aaatgcagca cccgcagcgg actcgacccg
      421 ggcggcagaa tcggcacctc gaaccgccgt aaccgcggcg agcgccgttc gatccctgac
      481 gcgtatccga tggcggcact gccaagggcg agggcaccca gtgggacggc ggtgagtcgg
      541 cgcatagcga caagcttcgc agactgtgcg gtgagagact gcggacatgt ccactgcccc
      601 gctcaagacg cgcctgcacg acgacctcac cacctcgatc aaggctcgcg acgagttgcg
      661 caccgcgacg ttgcggatgg ttttgtctgc tatcaccaac gaggaagtgt cgggcaccga
      721 agcccgccag ctgtccgatg acgaggtgct gaaggtactc acccgcgagg gcaagaagcg
      781 gcgcgaggcg gccgaagcgt tcgccgccgg caaccgcccc gagctggctg agcgcgagcg
      841 cgcggagggc gaggtcatcg ccacctacct gccagcgcag ctatcggacg acgaattgcg
      901 cgacctcgtg acgcaggccg tcgcctctgc cggggcgagc gggccgcaag cgatgggcgc
      961 ggtcatgaag gtcgtgaatc cgctggtcgc cggccgggcg gacggcgggc gggtggccgc
     1021 cgaggtaaag cgccagctcg cgggctagcg caccagctcg aagggcgtca gggctagtgg
     1081 gtcggggtac tggtcgggct cggcgttacg gtcggcgtcg gggtgccacc cgtaatggtg
     1141 atcggcgacg gcgagcccgg gatctgggag ctcggcggct ctgagctcgg tggctccgag
     1201 gatgccggcg gcgacacgaa cggcgggatg ccgttactga gctcgatctt gaccaatgct
     1261 ccggcgtaca cgatcgtgcc ggacgcgggg atggtggtct cgaccttccc cagtggctgg
     1321 ctcgacgtga ccttcgtcgg gtcgactgct gggctaaggc ccagcttggt cagggccgcg
     1381 gcggcggccg ccgggtcctg tccctgcaga ttcgggatcg ttatggccgg ccctcgcaac
     1441 acggtgtccg gtgggttctg gaagctcgcg atcggttgat tgagcagggc acccttcatc
     1501 gcgagctgcc agatcggacc gggcagcgtc gcgccgtaga cgtatgcgta cttcttgttg
     1561 ttgaccgtga tggcctgcat cggcacggtc gagtcggggt tgccgaccca caccgacgag
     1621 gccagattcg gtgtgtagcc gctgaaccac gcgttcttga actcgtcgtc agtaccggtc
     1681 ttgcccgcgg ccggacgacc cagtgcgagg ccatacccgg tgccgccctg gcccaagacg
     1741 ccctgcagaa gcgtggtgac tccctcggcg atcgacgggt cgagcacctt tccgcagttg
     1801 ggtcccggga ccgggcggtt cgtcgtcacg tcgccgacct gccaggtgac cttggtgatc
     1861 gactgtgggg tgcagaacgt gccgttgttg gccaggcccg cgaatgcgcc ggtgacgctc
     1921 agcgggtcga ccggctccgc accgagggtg aacgccggga tctggtcgag gtcgccgccg
     1981 gtcgccttgt gtacgccgag atcctgggcg atcgtgacgg gcgcgcacag gccggtcttc
     2041 acttcgagct gggcgaagta ggtgttcacc gactgcgcgg tcgcctgcac catgttgaac
     2101 gtgccgccct cagagtcctc ggcattcacc acgttccaca ccgaggtggg cagcccgtcg
     2161 cacgtcgtgg tgctgccctt cgggatggtg atcttggccg gcgacttgat cgtcgtcccg
     2221 gtcgggatct tctcctccaa cgccgctgcg aggacgaaga tcttgaacgt cgaccccgcg
     2281 ttgcggccct gcgagccgcc gtggttgacg tagtcggccg ccaggttcac ctcggtctgc
     2341 ccgaatccgg ttccgtacgg ccggtcgacc gccatggctt cgatcgcgcc ggtctgggga
     2401 ttgaccatgt cgatcgccgc cgccagaccc gcgttgaggg ccggactctc cggaacgacg
     2461 gtgtcgaccg caagctgcgc ggccttttgg acggcgggtt gcagcgtggt cgttacgacg
     2521 agcccgccgc ggcgcagcag gttcgcccgg tcagtgacgg tggcgccgaa ctcgggaccg
     2581 ttcaggatgt cgtgcacgac gtagtcacaa aagaacgggg cggacgacgt cgcgcagccg
     2641 ttcgggaccg ggacgatggt caagcccagc ggcgacgcct ttgctgcgtc gatctgggtg
     2701 gaggtgtagc tgtccgggta gttgaccagc atccggtcga gcacgacatt gcggcgggcc
     2761 agcgcatcct tcgggttcac ggtgggatcg aacgtgctgg ggttgttgat cacgccggcg
     2821 agcaacgccg actgcgacag cgtcagctgg tcggcggtct cgccgaaata gtgctgcgcg
     2881 gcggccgaga tgccgtaggc gccgtcgccg aagtagacgg tgttgaggta gtcctcaagg
     2941 atctggtcct tcgtcagctt ctgctcgagc gccagcgcat atcgcgcctc ggtgagcttg
     3001 cgggtcaggt tcttggaggt cgcggcggcg gcggcggcag tgttgttgtc ctgcgcgttc
     3061 gtctcgacca gcgcctggcg cacgtactgc tgggtgatcg tcgacgcgcc ctgcaccgcg
     3121 ccgcccgacg aggtgtcggt caccaacgcg cgcaagatgc ccttcgggtc gatgccgtgg
     3181 tgctcgtaga agcgggagtc ctcgatcgcc acgatggcct gccgcatggt aggggcgacc
     3241 gcatcgaggc tcaccgactg ccggttctcg tagtaaaagc tggccagggg cgtcccgtcg
     3301 gcggcattga tcaccgagag ctgtgacagc ggcggcgact gaagggacgc cgggaggtcg
     3361 gcgaaggtga tgtcacctat cgcgttcttg gtggtgatcc cgagaccggc cgcggcgggc
     3421 aacgcaaggc cggccaccag gaggccgacg agcagcgaga gcacccccag catcagagcg
     3481 cgggccaggc gtccgccgat gctaatccgc ccatctggca ttcctgcagc gtaaccgctg
     3541 ggcagggatg actaggccgc tcacccggat ggctcggctg ctgtccatcc gtacgagcgt
     3601 gaaccgaccg tacggaggct gttgagggcg tctcctcagt ccgtaacgtc gggggatcga
     3661 gcccacctgg ggacggtagc tcgaggaatc cacagcaacg gacggggctc ccgatggctt
     3721 ggacaacaga ctggaccacc cgcgcagggt gtcgaaacgc cgatccggac gccttgttcg
     3781 tccagggcgc cgcgcagaac cgagctaagg ccgtttgctt cggctgcccg gtccgggctg
     3841 agtgcttggc ggatgctctc gacaaccgtg tcgagttcgg cgtgtggggt ggcctgaccg
     3901 agcgcgagcg ccgcgctctg ctgcgccgcc gcccgaacgt cacctcgtgg gcgaatctgc
     3961 tcagcaccgc gatgacggac ttcgagcgcg acgccgccga ggcctactcc gcgaccggct
     4021 gaaaccctcc gctacgcggt ttcgcacaac gcgtcgccga gcgtgcgcag gccgttcagg
     4081 tcgtgcacat cctgggcaag cgctcgcgcc gacgccagca gcacattcgg gtgcgcgagc
     4141 gcgaaaccgt cgactcggcg ttgctcagtc gcctgcatcg tcaaccgctc cgcgtgcacc
     4201 cgaagcacgc cggccgtcag cgcgccgtcc agatcgccgt ccgcatcgtc ttcgccgtcg
     4261 agggcttccg ctgccgatag cgcattgacc gccgtgatct gcggcaccgt gctgcgatgc
     4321 acccggttca cgacgacacc cgcgagcggc atgtcttcct cgaccagccg gtcgacgaaa
     4381 tacgatgcct cgcgcaacgc atcgggctcc ggcgacgcga cgaccacgaa gaccgtgccg
     4441 ggcgcccgca gcagcttcgc agtggcgtcg gctcgctgtc gcaagccgcc gaacatcgtg
     4501 tccatagcgg caacaaaagt ctggacatcg gtcagcaact gcgttccgag cagcttcatc
     4561 atcgttccgg tgaccagccc tagcccgacc tgcaccactt tcgtgtatcc gcggccgccg
     4621 actttcgccg gcactagcag catccggatg aaccggccgt cgagaaagcg cgccaaacgc
     4681 gcgggtgcgt caaggaaatc gagcgccgaa cgcgcaggcg gcgtgtcgac gacgatgaga
     4741 tcccactggc ccgaggcgcg tagctggccg agtttctcca tcgccatgta ctcctgcgtg
     4801 ccggcgaagc tcgatgacag cgattgataa aatgggttcg ccagaatctg cgcggctttc
     4861 gccggtgatg cgtgtgcctc gacgatctcg tcgaacgtgc gcttcatatc gagcatcatc
     4921 gcgtgcagct cgccctcgtt ctttacgccg tccgcacgct tcttcagctt catgcctgcg
     4981 accttgcgcg gcacattgtc gagctcttgg aggccgagcg actgtgcaag ccggcgagcc
     5041 ggatcaatgg tgagggtgac aacgcgcctg ccctgttcag cagcgcggac gccgagggca
     5101 gccgccagtg tcgtcttgcc gacgccaccg gatccgcaac agacaatgat tttcgtgccg
     5161 cggtcggcaa tcagagtttc gatgtcgagg tgggtcatgc ggcgccctgc tcgacgagtt
     5221 cagcggcgag ggcgtacaga cgaccgacat cggtggtgtc gaggtcgtgg agtcgatagg
     5281 tcggccgcgc ggccgcgcgc aggcggctac gcagcttgcg ttcgaccgcc aactgcaggg
     5341 catgctcggc cgcttccatc gacagtcctg cggtgatcgc atctcctgct ggcagtcccg
     5401 ctgacttgag gccccgcgcc aacgccgtga gatcgatggc gccagcaccg gctgccttca
     5461 actcggacgt cggtaacagc ggctcgtgca cccggttcac gaagatgccg ccgaccggaa
     5521 ggtcgaactc gtgcagttct tcgatgccat cgagggtttc ctgcacaggc atctcttcaa
     5581 gcatcgtgac gagatggacg acggtctgcg gcgatctgat cagccgcatg atggattcgg
     5641 cttggcggtt caccggaccc actcgagcaa gactggcgac ttcgctgttg acgttgagga
     5701 atcgcgcgat gcgccccgtg ggcggcgcgt ccatgacgac cgcgtcatag acaaagtcat
     5761 cggtcacgtc aaatgctcgg tcacccggcg acgtccgcgt tgatttgtgt gttgttcgtc
     5821 ttcgcgttgc ttcgtagacc ttgccggtga gcaggacgtc gcgaacgcca ggtgcgatcg
     5881 tggtcgcgaa gtcgatggcg ccgagtcttc gcagcgcgcg gccggcgcga ccgaggtggt
     5941 agtacatctc gaggtattcg agcagggctt cctcggggtc gatcgcgagt gcgtagacga
     6001 cgccgccgtc gggtgcgacc gccacgcgcc gttcggcgta gggcagcggc gggctgtcga
     6061 aaaggcgcgc caagccttgg cggccttcgc attcgacgag gagggtgcgg cgtccgcccg
     6121 ttgcgagggc taacgcgagt gcggccgcga cggtagtttt gccggtgccg cccttgccgg
     6181 tgacgatgtg gaggcggacg ccgtcccaat cgcgatcgcg cccgctgtcg cctcgctgcg
     6241 cgttcactcg tcgagcgtag ccattaggct cgcgtccatg accaagtggg agtacgcgac
     6301 tgtgccactg ctggtgcacg ccaccaagca gatcctcgac aactggggca gcgacggctg
     6361 ggagctcgtg agtctcgtgc ccgggccgaa ttccgagcag ctggtggcct acatgaagcg
     6421 gccggtcgaa tcgtgagcac gcctagcctg acgacgcccg aggatcggct catcgccatg
     6481 gggctcacgt tgcccgcggt ttccgcaccg cttgcggcgt acgtaccagc gctgcggtca
     6541 ggaaattacg tgtacacgtc gggtcagctg cccacggtgg acggcgagct ggtagcggtc
     6601 ggcaaggtcg gcaccggccc cggcgcatcc gtatcgcccg aggaggcgaa cgcggccgcc
     6661 cgcatttgcg cgctgaatgc gttggcggcg ttgcgcagcg tcgtcggaga gctgtcgtcg
     6721 gttgtgcgca tcgtgaaggt cctcggcttt gtcgcgagcg cgccggactt caccgcccaa
     6781 gctgcggtta ttaacggggc gagtgagtta ctgctcgagg tgttcggcac cgcgggtggg
     6841 gctgctgccc caatcggctc gcacgcgcgc agcgcggtcg gggtcgccgt cctgcctcgg
     6901 gacgcacccg tcgaggtcga actcatcgcc gaggtcaccc ccgcgtagcc cgttcgcgat
     6961 ctgggccagg ccctaccgat tcgaacgtgt gttcgagtag gatctccgag tatgacgccc
     7021 ggcgggcagc cgacgatcct gcacgctgac ctcgacgcgt tctatgcgtc ggtcgagcag
     7081 ctcctcgatc cgtccttgcg cgggcggccg atcgcggtcg gtggcggagt ggtgctggcg
     7141 gcctcctacg aagcgcgcgc cttcggcgtc accggtggga tgtccgggcg tcgagctcgc
     7201 caactgtgcc caggtctgcg gttcgtcggt ggccatttcc acgagtacca gcgcctcggc
     7261 gatcaggt
//
LOCUS       DATCBJ010000404         2633 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_296743, whole genome shotgun sequence.
ACCESSION   DATCBJ010000404 DATCBJ010000000
VERSION     DATCBJ010000404.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2633)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2633)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2633
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_296743"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            212..1543
                     /locus_tag="VII50_08125"
     CDS             212..1543
                     /locus_tag="VII50_08125"
                     /inference="COORDINATES: protein motif:HMM:NF013752.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEY5050854.1"
                     /translation="MRELPADLAALDVLLRDRALLAPITAHWLCEAATGRASVGHGRP
                     TIAIETYVRLMVVKHRTGWGYETLVREVSDSLHLRRFCLIALGERVPEESTVRKLTRR
                     LGGAVIDELTRLVIAKAARETRFRAGAVRIDSTVVQADVRYPTDSGLTLDGVRVLGRE
                     SRRVTLKVGADVGRVVDRSRALGRRLRLISRTLARRTGERKAEVLALTEQAGRLLSRS
                     IREARQLALAARRRARGRGARAKLAAIERLEEMTDRAQRVATQIQQRLAGEAITDRLV
                     SMFDPDARPIRKGKLGKPNEFGYVTQLAEVTENTKRGARGFILPPSMVVGNPQEHTLL
                     PTTISELDRLGLRPREVALDGGFQGPLIEQALNDLAPHRVFIAGRQQPWSRRTQRRLA
                     RYRVGAEGRISHLKREYRLDRSRLKGEHGTRAWNAWAIATYNLDTLAIRTT"
     gene            complement(1650..2324)
                     /locus_tag="VII50_08130"
     CDS             complement(1650..2324)
                     /locus_tag="VII50_08130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5050855.1"
                     /translation="MPVTPTTGPATLKSTQAYSVNIDTKDANGLHVLTDVVRLAPLPA
                     AESPELIFLGVLQGGKKAAFLFTNSVKVSNQSGSQLTCLPSPDDCQIVELSPGQGMKL
                     APTSNTALITTFTFELMSIGATNYSSAAAAKQAREAVSTAGQTLVPLSNSTELQTLRF
                     DDKIGALVHQNVPASPPSGTTGSTGSTAATGATGSSGSTGATGGTSGNAERFPNGVAF
                     VLSPVG"
     gene            2314..>2633
                     /locus_tag="VII50_08135"
     CDS             2314..>2633
                     /locus_tag="VII50_08135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5050856.1"
                     /translation="MTGTAGVTPELPEEPLGVVDPDLGLVFAGVVFAGVVFAGVVFAG
                     VVFAGVDVLVDFDLVVAAAFEGVDPAGVEPPDDPPASRNGFGSAPRRVSDVAGTTVVS
                     DASS"
BASE COUNT          376 a          855 c          955 g          447 t
ORIGIN      
        1 cggggaacgc cgagcgtttc ccgaacggtg tcgcgttcgt gctcagtccc gtgggctaac
       61 tacttgcccc ggatttagtc gggtttctgt ccgtctcggt ttggagaatg ggtcctgcaa
      121 atggacgttt ttctggccga ggaggacgag aatgttgcgg ctgtcgggtg ggcaggtcga
      181 gtcgttgtgg gacgaggttt tgccggttgg ggtgcgggag ttgccggcgg atttggcggc
      241 gttggatgtg ctgttgcgcg accgggcgct gttggcgccg atcacggcgc actggctgtg
      301 cgaggcggcg acgggacgcg cgagcgtggg tcacgggcgt ccgacgatcg cgatcgagac
      361 gtatgtgcgg ttgatggtcg tcaagcaccg cacgggctgg gggtacgaaa cgctggtcag
      421 ggaggtctcg gactcgctgc atctgcggcg cttctgtctg atcgcgctcg gcgagcgggt
      481 gccggaggag tcgacggttc gcaagctgac gcgccggctt ggcggtgcgg tgatcgacga
      541 gctgacgcgg ttggtgatcg ccaaggcggc gcgcgagaca cgttttcgcg ccggcgcggt
      601 gcggatcgat tcgaccgttg tgcaggcgga cgtgcgctac cccacagact ccgggttgac
      661 gttggacggt gtccgggtgc tggggcggga gtcgcggcgt gtgaccttga aggtcggcgc
      721 ggatgtcggg cgggtggtgg accgctcgcg ggcgctcggg cgccggttgc ggctgatctc
      781 gcgcaccctg gccaggcgca ccggggagcg caaggccgag gttctggccc tgaccgagca
      841 ggccgggcgg ctgttgtcgc gctcgatccg cgaagcacgc cagctggctc tcgcggcgcg
      901 ccggcgtgcc cgcggtcgcg gtgcccgggc gaagctcgca gcgatcgagc ggctcgagga
      961 gatgaccgac cgcgcacagc gcgtcgccac ccagatccag cagcgcctcg ccggcgaggc
     1021 gatcaccgac cgcctggtct cgatgttcga ccccgacgcc aggccgatcc gcaagggcaa
     1081 gctcggcaag cccaacgagt tcgggtatgt gacccagctc gcggaggtca ccgagaacac
     1141 caagcgcggc gcgcgcgggt tcatcctccc gccctcgatg gtcgtcggca acccgcagga
     1201 gcacacgctg ctgcccacca cgatcagcga actggatcgc ctcggcctgc gcccacgcga
     1261 ggtcgccctc gacggcggct tccaaggccc tctgatcgaa caggcgctca acgacctggc
     1321 cccacaccgc gtgttcatcg ccgggcgcca acagccctgg tcgcggcgca cccagcgccg
     1381 cctcgcgcgc taccgcgtcg gcgccgaagg ccgcatcagc cacctcaaac gcgaataccg
     1441 actcgaccga tcccgactga aaggcgagca cggaacgcgc gcctggaacg cctgggcgat
     1501 cgccacctac aaccttgaca ccctcgccat ccggaccacc tgaaaccatc cacccgctcg
     1561 accagaaacg agcaccattt gcccggaacg gccgcgttga ccaacaacgc ggccgctctg
     1621 gttaacagcc gtaaatccgg ggcaagtagt tagcccacgg gactgagcac gaacgcgaca
     1681 ccgttcggga aacgctcggc gttccccgac gtgccgccgg tcgcgccggt cgatccactg
     1741 ctgccggtcg ccccggtcgc ggcggtcgat cctgtcgatc cggtggttcc actcggcggg
     1801 cttgccggga cgttctggtg gaccagcgcg ccgatcttgt cgtcgaaccg gagtgtctgc
     1861 agctcggtcg agttcgagag cggcaccagc gtctggccgg ccgttgacac cgcctcgcgt
     1921 gcctgcttgg cggcagcggc cgaggagtag ttggtcgcgc cgatcgacat cagctcgaag
     1981 gtgaaggtcg tgatcagcgc cgtgttcgag gtcggcgcca gcttcattcc ctgcccgggc
     2041 gagagctcga cgatctggca gtcgtcagga cttggcaggc aggtcagctg gctgccggac
     2101 tggttggaga ccttgacgct gttcgtgaac aggaatgcgg ccttcttgcc accctggagc
     2161 acgccgagga agatcagctc cggcgactcg gccgcaggca gtggcgccag gcgcacgacg
     2221 tcggtgagga cgtgcaggcc attggcgtcc ttggtgtcga tgttgacgga gtacgcctgc
     2281 gtcgatttga gcgtcgccgg gccggtcgtc ggcgtgaccg gcaccgcggg cgtgacaccc
     2341 gagctccctg aggagccgct cggcgtcgtc gatcccgact tgggcttggt cttcgcgggc
     2401 gtcgtcttcg cgggcgtcgt cttcgccggc gtcgtcttcg cgggcgtcgt ctttgccggg
     2461 gtggacgtgc tcgtcgactt cgacttggtc gtcgcagcgg ccttcgaggg ggtcgacccg
     2521 gccggagtgg agcctcccga tgacccgcct gcctcgcgga acgggttcgg ttccgcgccg
     2581 cggcgtgtga gcgacgtggc cgggacgacg gtcgtcagcg acgcgagctc ctt
//
LOCUS       DATCBJ010000405         2561 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_82727, whole genome shotgun sequence.
ACCESSION   DATCBJ010000405 DATCBJ010000000
VERSION     DATCBJ010000405.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2561)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2561)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2561
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_82727"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..341)
                     /locus_tag="VII50_08140"
     CDS             complement(<1..341)
                     /locus_tag="VII50_08140"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEY5050857.1"
                     /translation="MSSRSATGVASLIGAGVLWGTGGVTGRALTDRAHLSGIAVGAYR
                     LGLGGLLLVVVLLLTGGRPPVARAAWQRIAAVAGLAAIYQGAFFSAVALSSVSLATLV
                     AIGSSPVWVLV"
     gene            complement(362..1078)
                     /locus_tag="VII50_08145"
     CDS             complement(362..1078)
                     /locus_tag="VII50_08145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY5050858.1"
                     /translation="MTLLVVDDIEVRYGNIRALKGVSFEVAEGEIVALLGANGAGKTT
                     TQKTVSGMLRPSLGAIIYDGQRIDGIPAHDLIRLGICHVPEGRHVFPRMTVAENLEMG
                     AFRFKSVDQKDLDHVLELFPRLRERYKQQAGTLSGGEQQMLAIGRGLMGKPRLLLLDE
                     PSMGLAPLVVAQIFDIVHQVNREGVTVLLVEQNAAQALSLAHRGYVLETGEIVLSGTG
                     GELLADDRVRAAYLGEEIAS"
     gene            complement(1075..1989)
                     /locus_tag="VII50_08150"
     CDS             complement(1075..1989)
                     /locus_tag="VII50_08150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY5050859.1"
                     /translation="MTTVEESAAAGSAHADDIVFDVDNVTLRFGGVTSLNAVSLQMHR
                     GEILAVIGPNGAGKTSLFNSLTGVYTPQEGSIMLSGRAGDPPVSVLGMPTHKVNRLGV
                     ARTFQNIRLFPALTALENVKVGVETRQKSGPVAAMLGMPWQRREERESTASAYALLRR
                     VGLERRGNDLASSLAYGEQRRLEIARALGTSPGVILLDEPAAGTNPAEKRELAGLIKG
                     INVDDGIGVLLIEHDMKLVMSVADRIVVLNFGQKIAEGSPEQIQRNPAVVAAYLGTST
                     EQATEQAAHQPEVQLIEVATDEAEGENP"
     gene            complement(1991..>2561)
                     /locus_tag="VII50_08155"
     CDS             complement(1991..>2561)
                     /locus_tag="VII50_08155"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEY5050860.1"
                     /translation="HLGPLHVHWGLAQLQYWYLLLIFLLLIILAFRRLEQSRLGRAWA
                     AVREDEVAAQATGINTTKVKLAAFAIGASTSGIAGAFFASQIGVFTPDNFVLNNSILI
                     VAYVVFGGMGSLTGALAGAAMLTWLPEFLKYQVPPADKLMWIGAVVLLMMIFRPAGLI
                     PAKRRAAELHGLDVPSSAETLAVPASEGL"
BASE COUNT          484 a          837 c          819 g          421 t
ORIGIN      
        1 tacaccagca cccacacggg cgacgaccca atggcaacca gcgtggcgag gctcaccgac
       61 gacagcgcga cggcactgaa aaacgcgccc tggtagatag ccgccagccc tgctacggcc
      121 gcaatccgct gccaggccgc acgggcaact ggtgggcggc cgccagtgag gagcagcaca
      181 acgacgagca acagtccgcc aagaccaagc cggtaggcgc cgaccgcgat cccggacaaa
      241 tgagcgcggt cagtgagcgc gcgaccggtg actccgccgg taccccagag gacgcccgcc
      301 ccgatcagcg aagcgacgcc ggttgcggaa cgcgaagaca aggacagctc ggaggtgggc
      361 gtcagctggc gatttcttcc ccgaggtagg cggcccgaac gcggtcatct gcgaggagtt
      421 caccaccggt gccggacagg acgatctcgc cggtttccaa gacgtacccg cggtgggcga
      481 gcgagagcgc ttgagccgcg ttttgttcga ccagcaagac tgtgacgcct tcgcggttga
      541 cttggtggac gatgtcgaag atttgggcga ccacaagcgg ggctagtccc atcgacggtt
      601 catcgagcag gagcagccgg ggcttgccca tcagccctcg cccgatcgcc agcatttgct
      661 gctcgccacc agacagcgtg cccgcctgtt gcttgtatcg ctcgcgcagc cgcgggaaga
      721 gctcgagcac gtggtcgagg tctttctggt cgacagactt gaagcggaac gcacccattt
      781 ccaggttctc ggcaaccgtc atgcgaggaa acacgtgccg accctcgggg acgtggcaga
      841 taccgagccg gatgaggtcg tgggcaggaa ttccgtcgat gcgctgaccg tcgtagatga
      901 ttgcaccgag cgacggccgg agcattccgg acaccgtctt ttgcgtcgtg gtcttccccg
      961 cgccgttggc gccgagcagg gccacgatct ctccttcggc aacctcgaag gacacccctt
     1021 tgagagcgcg gatgttgccg tagcgcacct cgatgtcgtc aaccacaagt agcgtcatgg
     1081 gttctcgcct tccgcttcat cagtcgcgac ctcgatcagc tgcacctcgg gttggtgcgc
     1141 agcctgctct gtggcctgct cggtcgaggt cccgaggtag gccgcgacga cggctggatt
     1201 gcgctggatc tgttcgggtg aaccctcagc gatcttctgg ccgaagttga gtacgacgat
     1261 tcggtcggcc acggacatga ccagcttcat gtcgtgctcg atcagcagca ccccgatgcc
     1321 gtcatcgacg ttgatgccct tgatcaagcc ggccagctcg cgcttttcgg cagggttggt
     1381 accggccgcc ggctcatcga gcaagatcac gcccgggctg gtgccgagag cacgtgcgat
     1441 ctccaaccgg cgttgctcac cgtacgcgag cgaggatgcc agatcgtttc cgcggcgctc
     1501 gagaccgacc cgccgcagca gggcataagc gctggccgtg ctctcacgct cctcgcgccg
     1561 ctgccagggc atgccaagca tggcggcgac gggaccggac ttctgccgcg tctcgacacc
     1621 gaccttcacg ttctcgagcg cagtcagcgc cggaaaaagc cggatgttct ggaaggttct
     1681 cgcgaccccg agccggttca ccttgtgggt gggcatgccg agcacgctga ctggcggatc
     1741 ccccgcgcga cccgagagca tgatcgagcc ctcttgcggg gtgtagacgc cggtgaggga
     1801 gttgaacagc gacgtcttgc cagcgccgtt aggcccaatg accgcgagaa tctcgccccg
     1861 atgcatctgc agggataccg cgttcaggct ggtgaccccg ccgaagcgca gcgtcacgtt
     1921 gtcgacgtcg aagacgatgt cgtcagcatg tgccgagccc gctgcggccg attcctcgac
     1981 ggtcgtcatg tcacaacccc tccgacgccg ggaccgcaag cgtttcggcg ctcgacggga
     2041 catcaagacc gtggagctcg gcagcccgtc gctttgccgg gatcagcccg gccggccgga
     2101 agatcatcat caacagtacg acggcgccaa tccacatgag cttgtcggcc ggtggaacct
     2161 ggtatttcag gaactccggc aaccaggtca gcatcgccgc accagctagc gcaccagtaa
     2221 gcgatcccat gccgccgaac accacatagg caacgatgag aatggagttg ttgagcacga
     2281 agttgtcggg tgtgaacacg ccgatctggc tggcgaagaa ggcgcccgcg atacccgacg
     2341 ttgaggcgcc gatggcaaag gcggcgagct tcaccttcgt cgtgttgata ccggtagcct
     2401 gcgcggcgac ctcgtcctct cgcaccgccg cccaggctcg cccgagccgc gactgctcga
     2461 gtcgccggaa cgccaaaatg atgagcaaca agaagatcag cagcagatac cagtactgca
     2521 gctgcgcaag cccccagtgg acgtgtagcg gcccgaggtg g
//
LOCUS       DATCBJ010000406         3038 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_43358, whole genome shotgun sequence.
ACCESSION   DATCBJ010000406 DATCBJ010000000
VERSION     DATCBJ010000406.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 3038)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3038)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3038
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_43358"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..271
                     /locus_tag="VII50_08160"
     CDS             <1..271
                     /locus_tag="VII50_08160"
                     /inference="COORDINATES: protein motif:HMM:NF013798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="macro domain-containing protein"
                     /protein_id="HEY5050861.1"
                     /translation="HAVGPRYGVDEPAAELLSGAYAQSLLRCDEVGATSVAFPALSTG
                     AYRYPLTESCQISVAALKATTTQVERCVLVAYDANTQELWQRALG"
     gene            537..1625
                     /locus_tag="VII50_08165"
                     /pseudo
     CDS             537..1625
                     /locus_tag="VII50_08165"
                     /inference="COORDINATES: protein motif:HMM:NF012308.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="serpin family protein"
     gene            complement(1656..2429)
                     /locus_tag="VII50_08170"
     CDS             complement(1656..2429)
                     /locus_tag="VII50_08170"
                     /inference="COORDINATES: protein motif:HMM:NF019359.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEY5050862.1"
                     /translation="MTDNGYPIGELAALANVTVRTLHHYDEIGLLHPSGRTVAGHRRY
                     GREDLDRLRQILVYRELDFGLDAIASMLADPGSGIEAHLRTQHRMLRSRIDRDQALLR
                     ALEKELEARTMGMALTPDEQFEVFGTDKIGGEWAAEAEERWGDTDAWRESQRRTARYS
                     KEDWVAMKSQSDAALRSYADAMTAGVPAASAHAMALAEDNRRFISRWFYDCSHTMQRN
                     LAEMYIADERFRGTYDTVMPGLALYVHDAIVANADAHDA"
     gene            complement(2476..2733)
                     /locus_tag="VII50_08175"
     CDS             complement(2476..2733)
                     /locus_tag="VII50_08175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5050863.1"
                     /translation="MVALLALLAIDSSVVGDTAMQASGGALACPPSARVVPARGTISY
                     HLDAQPYDQGDPGDGANHGSIATARFAIASIPMARAILRVN"
     gene            complement(2839..>3038)
                     /locus_tag="VII50_08180"
     CDS             complement(2839..>3038)
                     /locus_tag="VII50_08180"
                     /inference="COORDINATES: protein motif:HMM:NF037170.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY5050864.1"
                     /translation="MPKIGKDRLHFDLPPPAADDQNAEVDRLVSLGAKRVDIGQGEVS
                     WVVMADPDGHEFCVRPPVIHT"
BASE COUNT          537 a         1004 c          963 g          534 t
ORIGIN      
        1 ccacgccgta ggccctcggt atggcgtgga tgaacctgca gccgaactgc tgtccggcgc
       61 ttacgcccag agcctcctgc ggtgcgatga agttggcgcc acgagcgtcg ccttcccagc
      121 actatcaact ggcgcgtaca gatacccgct gacggagtct tgccaaatca gcgtcgcagc
      181 gttgaaggca acgacgactc aggtcgaacg ttgcgtactc gtcgcgtacg acgccaacac
      241 tcaagagttg tggcagcggg cgcttggatg agacggaacc agcgcgcgct gcccggcgtc
      301 caaccgctgt gaagcgatct ttgagccggg cgggcctcgc gctcgtcgtc gcggctacgc
      361 tggccgcgtg cgcgagttcg accacgaaag cgggcgacgc gagaaccccg agtgcggtca
      421 acgcgacaac gcccactgag gtgagagcca ccgaggtggc tcgcgctgat ccgggttcat
      481 tgtcgactgc gaccctcgtc gaaggcatca acacgttcgg ccacgacctt tacacagtgg
      541 ccgccactgg cggcaacgtc gttgtatccc ccttgagcat cgaagtggcg atgtcgatgg
      601 cgcgcgccgg cgccggcggc acgacggccg ccgagatcga cgcggcgatg cacttcccga
      661 gcgccggacg tgacccacag atgaacgcac tcacgcgcga tctggccacg caggacaccg
      721 cgccgccggt gcaatcgccc gccgcgagtc gcgacccgaa cgcacctccc gcggcaccaa
      781 tcctgactat cgccaacggc atgttcgtgc agcagggcca atcgatcggg caagcattcc
      841 tgaagacgat ggccgccgac tacggctcag gtgtccgtac ggtcgacttc acatcgccgt
      901 ccgccattgc gcagatcaat gcctgggtgc gcacgcagac cgccaatcgg attaagcaac
      961 tcttcgactc actcgatcca tcgacgaagc tcgtgctcgc caatgccgtc tatctgaaag
     1021 ccgactgggc gacaccgttc gcagagcagc cgacgtccga cgcaacgttc acgacggggg
     1081 ctggcaagca gatcacggtg ccgacgatgc accaagacgg gtcgatgggc tacgccagtt
     1141 cgaacaagtg gcaggccgtc gcgctgccct acgccggagg cacactgacg atgtacgtca
     1201 tcgtgccgaa cgcgagcaca cccctcgctg acctgctcgc gccggccacc ttgacgcgga
     1261 tctccgacgg gctccgtcct acttctctga gtctcgcgct gccgcgctgg acgtcgacga
     1321 ccaagctcga cttggcgccg gtgctcaaca cgctcggcat ccggaccggg ttcaccccca
     1381 cggccgactt ctctgcgatc gcgccaggtc tctacataac gcaggccatc caccgcgcga
     1441 ccatcgacgt ggacgagtgg ggcacctaag cggccgcggt taccgggctc gcgttcgctg
     1501 cggcatcgag ggcggcgccg ccgctgaccg tccgcgccga ccacccgttc gcgtacgcga
     1561 tcgtgaaccc gcagaccggc gtcgtcgtgt tcgaaggatc ggttgccgac ccgtcggctg
     1621 gctgaccgga caacgacagc tcggcttgta cggatctagg cgtcgtgggc atcggcgttg
     1681 gcgacgatcg cgtcgtgtac gtatagcgcg agtcccggca tgacggtgtc gtaggtgccg
     1741 cgaaaccgtt cgtcagcgat gtacatctcg gcgaggttgc gctgcatggt gtggctgcag
     1801 tcgtagaacc agcggctgat gaatcgccgg ttgtcctcag ccaacgccat ggcgtgcgcg
     1861 cttgctgcgg gaacgccggc ggtcatggca tcggcgtacg agcgcaacgc ggcgtccgac
     1921 tgtgacttca tcgctaccca gtcctctttc gaatacctcg cggtgcgtcg ctgggattcg
     1981 cgccaggcgt cggtatcgcc ccagcgctct tccgcttcgg cggcccattc gccaccgatc
     2041 ttatcggtgc cgaacacctc gaactgctcg tccggcgtga gtgccattcc catcgtgcgg
     2101 gcctccagtt ctttttcgag cgcgcgcagc aacgcctggt ctcggtcgat gcgggaacgc
     2161 agcatgcgat gttgggtgcg caggtgagcc tctatgccgg atccgggatc ggcgagcatc
     2221 gatgcaatgg cgtcgaggcc gaagtccaat tcccggtaga cgaggatctg ccgtaaccgg
     2281 tcaaggtctt cacggccgta cctgcgatga ccggcgaccg tgcgcccgct cggatgcagc
     2341 agtccgatct cgtcgtagtg gtgcagcgtg cgcactgtca cgttggcgag agcggccagt
     2401 tcgccgatcg ggtatccatt gtcggtcatg ggaccactgt gcaacctcac gtcacgtcag
     2461 gtgcaagcgg aatcgctaat tgacgcgtag gatggcacgc gccatgggaa tgcttgcgat
     2521 ggcgaagcga gcggtggcaa tgctgccatg attggcgccg tctcccggat cgccttggtc
     2581 gtatggctgc gcatcgaggt ggtagctgat cgtgccgcgt gcggggacga ctcttgccga
     2641 tggcgggcac gctaatgctc cgccactggc ttgcatcgcg gtgtcgccca ccacggaact
     2701 gtcgatagcc agaagcgcca gcagggcaac caacctggct tcacacggct gagacggaac
     2761 gtcgacgatg caggttccag aaaccagtct gaccgcacca gcatcggatt tactgggagc
     2821 cgtgccgacc tgcttgagtc acgtatgaat gaccggcggt cgcacgcaga actcatggcc
     2881 gtcgggatcg gccatcacaa cccaactgac ctcgccctga ccgatgtcga ctcgcttcgc
     2941 tccgagtgaa acgagtcggt cgacctccgc gttctgatcg tcggccgcag gtggagggag
     3001 gtcgaagtgc agccggtcct tgccgatctt cggcatca
//
LOCUS       DATCBJ010000407         3404 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_9113, whole genome shotgun sequence.
ACCESSION   DATCBJ010000407 DATCBJ010000000
VERSION     DATCBJ010000407.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 3404)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3404)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3404
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9113"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1209)
                     /locus_tag="VII50_08185"
     CDS             complement(<1..1209)
                     /locus_tag="VII50_08185"
                     /inference="COORDINATES: protein motif:HMM:TIGR00711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HEY5050865.1"
                     /translation="MAGRGRPLATFIVTSVALFMSSLDNLVVTMALPAIKTHLHASLS
                     SLEWTVNAYTLTFAVLLLPGATLGDRFGRKKVFMIGLSIFTTASALAALSSTAGELVA
                     ARALQGVGGAILTPLTLTLLSVAVPPARRGLALGAWGAIAGLAIAVGPVVGGAIVEGA
                     SWSWIFWLNVPIGIALLPVAGRLLKESNGPAAKLDAVGTGLATAGLFSVVLGLVKAND
                     WGWGSSSIIGLFVAGVVLLATFVRWETRVADPVLPLRLFRNRGFTASNVASLFMFFGM
                     FGSIFLLAQFLQVAHHYSPLSAGVRTLPWTAMPIFIAPVAGLLSDRIGGRRIVATGLF
                     LQAIGLYWLAATATPTVSYLHIVPGLAISGFGMALFFAPVANLVLSSVRHEEQGIASG
                     AHNAIRELGGV"
     gene            complement(1302..1841)
                     /locus_tag="VII50_08190"
     CDS             complement(1302..1841)
                     /locus_tag="VII50_08190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEY5050866.1"
                     /translation="MTAPQTRMDPDERRDQVIAAALVEFAEGGLAGTSTEAIAVRAGI
                     SQPYLFKMFGTKRDLFIAASKRCFDNVAQTFTRAADGLAGEEALDAMGHAYYGLIADR
                     ANLMAQLQMYAACHDLEVRECARDGFRKLYQLVERASGAPADTLAVFFAGGMLCNVST
                     ALGLAEIDEPWARALAGDK"
     gene            complement(1852..2340)
                     /locus_tag="VII50_08195"
     CDS             complement(1852..2340)
                     /locus_tag="VII50_08195"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY5050867.1"
                     /translation="MSNAVALLAAIGISIAFALRLCWISVFVGMIARTAGAVQGIMFL
                     LVLPLSFGSHVFVTTSTVPGRLQAFIKVNPLSHLVGTVRGLVIGAPVTDHLLWTFTRA
                     RYDADATIAAFSAGLRDAVDLERVQLELLEAVHRSLRPAGASVWIRAGSAGPVDKVVS
                     DH"
     gene            complement(2337..2459)
                     /locus_tag="VII50_08200"
     CDS             complement(2337..2459)
                     /locus_tag="VII50_08200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5050868.1"
                     /translation="MTRLAQAGIGVTELSLHLPSLDEVFVTLTGTRTADTEAVA"
     gene            complement(2496..2921)
                     /locus_tag="VII50_08205"
     CDS             complement(2496..2921)
                     /locus_tag="VII50_08205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009072195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HEY5050869.1"
                     /translation="MTTVTYVSVEVGEQLPHRSFPITRADLVRYAGASGDFNPIHWNA
                     RFAVGVGLPDVIAHGMFTMATAARLVTDWIGDPGAVVDYSVRFTKAVVVPDDEGGATL
                     DVEGVVAEKLDGGLVRIDLTARSDADKVLGMARATVRLS"
     gene            complement(2918..>3404)
                     /locus_tag="VII50_08210"
     CDS             complement(2918..>3404)
                     /locus_tag="VII50_08210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MaoC family dehydratase N-terminal
                     domain-containing protein"
                     /protein_id="HEY5050870.1"
                     /translation="PLVAGRAAPMPLDPSFVGRSYPPTAPYMVGREKIREFAAAIGDA
                     NPAYTDVEAARALGHPDLVAPPTFAIVLSMRASHQVVRDPALGLDYSRVVHGDQRFSY
                     TRPIVAGDELSVIVTVEKIRSMAGNDIITTRGDIVTTDGEAVCSAYSTLVARAPDEVP
                     E"
BASE COUNT          656 a         1110 c         1142 g          496 t
ORIGIN      
        1 cacgccaccg agctcacgga tcgcgttgtg cgcacccgat gcgatgccct gctcctcgtg
       61 ccgcaccgac gagagcacca ggttggcgac aggcgcgaag aacagcgcca tgccgaaccc
      121 gctgatcgca agaccgggaa cgatgtggag gtacgacact gtcggcgtcg cggtcgctgc
      181 cagccagtag agcccgatgg cctgcaggaa gagaccggtc gcgacgatcc gacggccacc
      241 gatgcgatcg gacagcaggc ccgcgaccgg cgcgatgaag attggcattg ccgtccatgg
      301 caacgtgcgc acgccggcgg acagcggcga gtagtggtgg gcaacctgca ggaactgggc
      361 gagcaggaag atcgagccga acattccgaa gaacatgaac agcgacgcga cgttgctcgc
      421 ggtgaacccg cggttgcgga acaaccgcaa tggcagcacc ggatcggcga cccgcgtctc
      481 ccaccgaacg aacgtggcca gcaacacgac gcccgcaacg aacagcccga tgatcgaact
      541 gctcccccag ccccagtcgt tcgccttcac cagaccaagg accaccgaga agagacccgc
      601 agtcgcgagg ccggtaccga cggcatcgag tttcgcggcc gggccgttgc tctccttgag
      661 caagcgaccg gcgaccggta gcaacgcgat gccgatgggc acgttgagcc agaagatcca
      721 cgaccatgac gcaccctcga caatggcgcc accgacgaca ggtccgacgg cgatggcgag
      781 gccggcgatc gcgccccacg cgccgagcgc gagtccgcgc cgggccggcg gaacagcgac
      841 gctcagcagc gtcagggtga gcggcgtgag gatcgccccg ccgacacctt gcagcgcgcg
      901 tgcagcgacc agctcccccg ccgtcgacga cagtgcggcg agtgccgagg ctgtggtgaa
      961 gatcgacaga ccgatcatga agaccttctt gcgaccgaac cggtcgccaa gggtcgcgcc
     1021 cggcagcaat agcacggcga acgtcagggt gtaggcgttc accgtccatt ccaggctaga
     1081 cagcgacgcg tgcaagtgcg tcttgattgc cggcaacgcc atcgtgacga cgaggttgtc
     1141 gagtgagctc atgaacagcg cgaccgaagt gacgatgaat gtcgccagtg gccttccgcg
     1201 tccagccatg tcatgtcctc cggttcggtc tgtaagtgag cagtcactca cctatgtggg
     1261 caaagaaaaa ccagtcacag caaggaaacg tggagtgcga tctacttgtc acccgcgagc
     1321 gcgcgcgccc acggttcgtc aatttcggcg aggccaagcg cggtagacac gttgcagagc
     1381 atgccgcccg caaagaagac ggcgagcgtg tcggccggcg caccggacgc gcgctcgacc
     1441 aactggtaga gcttccggaa cccgtcacgg gcacactcac ggacctctag gtcgtggcat
     1501 gccgcgtaca tctggagctg agccatcagg tttgcccggt ccgcgatcaa gccgtagtag
     1561 gcgtgaccca tcgcgtcgag cgcctcttcg ccggcgagcc catcggcggc ccgggtgaat
     1621 gtctgcgcga cgttgtcgaa acagcgcttg gacgccgcga tgaacaggtc gcgtttggtg
     1681 ccgaacatct tgaacaggta gggctgcgag ataccggccc ggacggcgat tgcttccgtc
     1741 gatgtcccag cgaggccacc ttcggcgaac tcgacgagcg ctgccgcgat cacctggtca
     1801 cggcgctcgt cggggtccat ccgggtctgg ggcgcggtca tgatggctaa gttagtggtc
     1861 actcactacc ttgtcaaccg gcccggccga tccggcgcgg atccagaccg aagcgcccgc
     1921 gggtcgcagc gaccgatgca ccgcctcgag aagctccaac tgaacgcgtt cgaggtcgac
     1981 ggcgtcgcgc agtccggcgc taaacgccgc gatcgtcgcg tcggcgtcat agcgtgcccg
     2041 agtgaaggtc cagagcaggt ggtcggtgac cggtgcgccg atcacgagtc cgcgcacagt
     2101 gccgacgaga tgcgacaacg ggttgacctt aatgaaagcc tgcagccggc ccggcactgt
     2161 ggacgtcgtg acgaagacgt ggctgccgaa cgagagcggc aacaccagca gaaacatgat
     2221 gccctgcacc gctccagccg tccgggcgat cattccgacg aaaaccgaaa tccagcagag
     2281 gcgcagcgcg aacgcgatcg agattcctat cgcggcaagc aatgcgacgg cgttgctcat
     2341 gcgacggcct ccgtgtctgc ggtccgagta ccggtgagag tgacgaagac ctcgtcgagg
     2401 ctcggcagat ggagcgacag ttcagtgacg ccgatgccgg cctgcgcgag tcgcgtcaca
     2461 gtgattaacg gggcgtaaca gcgcacgccc cggggtcagg acaggcggac cgtggccctg
     2521 gccatgccga gcaccttgtc ggcgtccgat cgagctgtca gatcgatccg gacgaggccg
     2581 ccgtcgagct tctcggcgac gacaccctcg acgtccaacg tcgcgccgcc ctcgtcgtct
     2641 ggcactacta ctgccttggt gaatcgcacc gaatagtcga ccacggcgcc cgggtcgccg
     2701 atccagtccg ttaccagcct ggctgcggtc gccatcgtga acatgccgtg cgcgatcacg
     2761 tcgggcaggc cgacgccgac cgcgaaccgc gcgttccagt ggatgggatt gaagtcgccg
     2821 gacgcgcccg cgtaccggac caggtcggcg cgggtgatcg ggaagctgcg gtggggcagc
     2881 tgctcgccga cctcgacgga cacgtaggtc accgtcgtca ttccggcacc tcgtcggggg
     2941 cccgcgcgac cagcgtcgag taggcgctgc agactgcctc gccgtcggtc gtgacgatgt
     3001 cgccgcgggt ggtgatgatg tcgttgcccg ccatcgagcg gatcttctcg acggtgacga
     3061 tcaccgacag ctcgtcgcca gcgacgatcg ggcgggtgta gctaaaccgc tgatctccgt
     3121 ggacgacccg cgagtagtcc aggccgagcg cggggtccct cacgacctga tgcgacgcgc
     3181 gcatcgacag cacgatcgcg aaggtgggcg gggctaccag atcggggtgg ccgagcgcac
     3241 gagcagcctc gacgtcggta taggcgggat tggcgtcacc gatcgcggcg gcgaattcgc
     3301 ggatcttctc gcggccgacc atgtatggcg cggtcggagg ataactacgg ccgacaaacg
     3361 aagggtccaa cggcatgggc gcggctcgcc ctgcgactag cggg
//
LOCUS       DATCBJ010000408         2495 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_187652, whole genome shotgun sequence.
ACCESSION   DATCBJ010000408 DATCBJ010000000
VERSION     DATCBJ010000408.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2495)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2495)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2495
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_187652"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..275
                     /locus_tag="VII50_08215"
     CDS             <1..275
                     /locus_tag="VII50_08215"
                     /inference="COORDINATES: protein motif:HMM:TIGR01552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="type II toxin-antitoxin system
                     prevent-host-death family antitoxin"
                     /protein_id="HEY5050871.1"
                     /translation="MSCDCGMSAIPARDLRNHTADVLRRVEPGEEIEVLRDNRPVAKI
                     IPLSWRRRWLPASEISRELLRLGPDTTGQAKELRETLTGTTDDLPW"
     gene            272..664
                     /locus_tag="VII50_08220"
     CDS             272..664
                     /locus_tag="VII50_08220"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HEY5050872.1"
                     /translation="MTPQRALADTSVFIGLEAERFDANQFANFEWGISVITLGELRRG
                     VLQAKEPEAASRRLSTYQLAQRFEALAVDESVADAWALLVSRLRAAGRKVPINDSWIA
                     ATAPAQHIPIVTQGAAYDLMPDAEVIKI"
     gene            complement(810..1832)
                     /locus_tag="VII50_08225"
     CDS             complement(810..1832)
                     /locus_tag="VII50_08225"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEY5050873.1"
                     /translation="MQYRTLGRTGIKVSPYALGALALGTPIGNPDHDDSARIIHKALD
                     AGINFIDTSDTYGESEVAVGKALKGRRDSVVLATKFSRPIGDDPNHQGASRRWIMTAV
                     ENSLRRLQTDYIDLYQVHRPDPATDIEETLSALSDLIHSGKVRAVGASAMPASDIIEA
                     QWVAERRGRERFRTDQPPYSILSRGIEREVLPVAQRYGMGILVWGPLGQGMLTGRVRK
                     GQQTDLRRAHIFRHLNDERRIDAVERLIPLAEKAGLPMTHLAITFAIAHPGVTSAIIG
                     PHTIEQLDDLLAAADVTLTDDVLDQIDEIVPPGTDVGMLDQAYLPPALQNSSLRRRPV
                     SERTAA"
     gene            1956..>2495
                     /locus_tag="VII50_08230"
     CDS             1956..>2495
                     /locus_tag="VII50_08230"
                     /inference="COORDINATES: protein motif:HMM:NF044515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEY5050874.1"
                     /translation="MTENTVPAGVGSVGSAGPGALERAPRRLRADAQRNIDSLLEAAK
                     AVFGTSGVDAPAKEIADRAGVGVGTLYRHFPQRSDLVKAVFQRGVDACADAAPALAAA
                     HEPGTALAKWLHRYTEFLATKRGLAAALHSGDPAFDALPGYFMRRLEPTLGSLLEAAT
                     ANGEIRADISPKYLLHAVAN"
BASE COUNT          382 a          782 c          898 g          433 t
ORIGIN      
        1 agatgagctg cgattgcggt atgagtgcga ttccagcgcg tgatctccgc aaccacacag
       61 cggacgtcct tcgtcgcgtt gagcctggcg aagagattga ggtgctcagg gataatcgcc
      121 cggttgcgaa gatcatcccg ctgtcctggc gccgccggtg gctgccagcg tctgagattt
      181 cccgcgagct cctgcgcctg ggacccgaca ccacaggtca ggccaaggag ttgcgggaga
      241 cgctgaccgg gacaacggac gatctgccgt ggtgacgccg cagcgagcgc tggcggacac
      301 gtcggtcttc atcgggcttg aggccgagcg gttcgacgcc aatcagttcg ctaacttcga
      361 gtggggcatc tccgtgatca cgctgggtga gctgcggcgg ggagtgctgc aggccaagga
      421 accggaagca gcttcacggc ggttgtccac gtatcagctc gctcagcggt tcgaggcgct
      481 ggccgtcgac gagtcagtcg ctgatgcatg ggcactgctg gtctctcggc tccgggcggc
      541 cgggcgcaag gtgccgatca acgacagctg gattgctgcg accgcgcccg ctcaacacat
      601 tccgatcgtg acgcaaggcg ctgcctacga cctcatgccc gatgctgagg tcatcaagat
      661 ctgaacgaga cgcgaaggcc gctgcctgag tgatgaggtg acgggcagcg gatctgatgc
      721 caccgcgcgt ctgcctccgt ctcatggcgg gggtggtgtc gcggtcagca gcagtcgtcc
      781 gccgtgggag accgggtgcg gggttgcggt caggcggcgg tgcgttcact cacgggtcgg
      841 cggcgcaaac tcgaattctg gagggccggg ggcaggtagg cctggtcgag catgccgacg
      901 tcggtgccgg gcgggacgat ctcgtcgatc tggtcgagga cgtcgtcggt gagcgtgacg
      961 tcggcggcgg cgagcaggtc gtcgagctgc tctatggtgt gcggcccgat gatcgcgctg
     1021 gtcacgccgg gatgggcgat cgcgaacgtt atcgcgaggt gcgtcatcgg caggcccgcc
     1081 ttttcggcga gcgggatgag ccgctcgacg gcgtcgatcc ggcgctcgtc gttgaggtgc
     1141 ctgaaaatgt gcgctcggcg caggtcggtc tgctggccct tgcggacgcg tccggtgagc
     1201 attccctgcc cgagcggacc ccacacgagg atgcccatac cgtagcgttg cgcgaccggc
     1261 agcacctcgc gttcgatgcc gcggctgagg atcgagtaag gtggctgatc ggtgcggaac
     1321 cgctctcggc cgcgccgctc agcgacccac tgggcttcga tgatgtcgga cgcgggcatc
     1381 gccgacgccc cgaccgcacg gactttcccg ctgtggatca ggtcggacag cgccgacagc
     1441 gtttcctcga tgtcggtggc ggggtccggc cggtggacct ggtagaggtc gatgtagtcg
     1501 gtctgcaggc ggcgcagcga gttctcgacc gcggtcatga tccagcgccg cgaggctccc
     1561 tggtggttgg gatcgtcgcc tatcgggcgg ctgaacttgg tcgcgaggac gacgctgtcg
     1621 cggcgccctt tgagcgcttt gcccacggcc acttcggact cgccgtacgt gtcggaggta
     1681 tcgatgaagt tgatccccgc gtccagcgcc ttgtggatga tgcgggccga gtcgtcgtgg
     1741 tcggggttgc cgatcggcgt gccgagcgcc agtgcgccga gcgcgtaggg actgaccttg
     1801 atgccggtcc ggccgagggt gcggtactgc atgcggcttc tcctgaggtt gcggtcgagt
     1861 gaacgctctt ccggtgccgt accctggacc gaagcggaac gttcttccgg aagaatacgg
     1921 aaagacgttc cggtaaagca aatgaaggag gtgccgtgac ggagaacacg gtgccggccg
     1981 gagtcggctc ggtcggctcg gcgggtccgg gcgcactcga acgtgcaccg cgccggctgc
     2041 gtgccgacgc gcaacgcaac atcgactccc tgctcgaggc ggcgaaggcc gtcttcggca
     2101 cgtcaggcgt ggacgcgccc gcgaaggaga tcgccgatcg ggccggcgtc ggggtcggga
     2161 cgctctaccg gcatttcccg cagcgttcgg acctggtcaa ggccgtgttc cagcgcgggg
     2221 tggatgcctg cgccgacgcg gccccggcgc tggccgcagc gcacgagccg ggaacagcac
     2281 tcgcgaagtg gctccaccgg tacaccgagt tcctcgcgac taaacgagga ctcgccgcag
     2341 cccttcactc gggcgatccg gcgttcgacg ccctgcccgg ctacttcatg cggcgacttg
     2401 agcccactct cgggtcactc ctcgaagccg cgaccgccaa cggcgagatc cgcgccgaca
     2461 tcagccccaa gtacctcttg cacgccgtcg ccaat
//
LOCUS       DATCBJ010000409         2403 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_245194, whole genome shotgun sequence.
ACCESSION   DATCBJ010000409 DATCBJ010000000
VERSION     DATCBJ010000409.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2403)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2403)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2403
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_245194"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..450
                     /locus_tag="VII50_08235"
     CDS             <1..450
                     /locus_tag="VII50_08235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5050875.1"
                     /translation="TYHFLRRLKPKALDVLTRFPLETAHLDAARIGAARTFAEFDERV
                     TAPMHGFANAEDYYRRASSLGYLGRIAVPTLCISSEDDPFFPREAVARARAVASPAVT
                     FQVTPWGGHTGFVSGRWPWRPRYWAEELAIDWLGARDRLGARRAVVA"
     gene            547..1365
                     /locus_tag="VII50_08240"
     CDS             547..1365
                     /locus_tag="VII50_08240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5050876.1"
                     /translation="MVAAVNVAIWLRVAVALQRDRALQGLERRRYRRRQLILSALFVG
                     GCAFRSIFPRADVQRICLYDNWLSVVLVGRAVATIAELAFMTQWALLLGESAGDGQRD
                     VAKIVSRVLVPIIAVAEICSWYAVVSTNFLGNAIEQSIWTFASVLVIVGLLSRERGAR
                     QRFVGIATALIGAYIVFMSMVDVPMYLRRWRADEQSRRPYLSFAEGVRDVGSRRVVTR
                     AWEPWREEVPWMSLYFSTGVWVSLWLIRAAPARKGSVTLGDENVTQSSLSKARS"
     gene            1417..2022
                     /locus_tag="VII50_08245"
     CDS             1417..2022
                     /locus_tag="VII50_08245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5050877.1"
                     /translation="MNGFFEELKEQRWDDHRFYHHSRINQSLHLVSAISFCVTYVLLF
                     KHPVVAALFGWTFAMCIRQVGHFFFEPKGYDVENQATHEYKERVKVGYNLRRKVILHS
                     LWALVPIALYFAPTALGAFTVPWTNLSTYLAHLARLWIGLAAGALLFRTIHLFFLRGV
                     KTGLVWFTKILTDPFHDIYLYHRAPLYLWRGELIEPAKQSA"
     gene            complement(2019..>2403)
                     /locus_tag="VII50_08250"
     CDS             complement(2019..>2403)
                     /locus_tag="VII50_08250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5050878.1"
                     /translation="YRNLDDTWNGRAERLNRRLVDDGLPVRLENLQTIWTVLYTQPGR
                     YNWMLQFYLRAQGLALSWVGTGRFIFSLNYSDADFDAVADKFLSAARAMQADGFWWSD
                     PALTDKAIKRRVLREMVAAAFGKGD"
BASE COUNT          367 a          773 c          789 g          474 t
ORIGIN      
        1 acctatcact tcttgcggcg gctcaagccg aaggcgctcg acgtgctgac acggtttccg
       61 ctcgaaacgg cgcacctcga cgctgcgcgc atcggcgcag cgcggacctt cgccgagttc
      121 gacgagcggg tcacggcgcc gatgcacgga ttcgccaacg cagaggacta ttaccgccgc
      181 gcgagctcgc tcggttacct cggacgtatt gccgtgccga ccctttgcat cagcagcgag
      241 gacgatccgt tttttccgcg cgaggcggtg gcgcgcgcgc gggcggtggc gtcgccggcg
      301 gtcaccttcc aggtcacgcc ctggggcggc cacacggggt ttgtctccgg gcgctggccc
      361 tggcgcccgc gctactgggc ggaggagctg gcgattgact ggctgggcgc gcgcgaccga
      421 ctgggcgctc ggcgagcggt tgttgcgtaa ctgtaactgg accgtcacgg aagtgccgcc
      481 gtatggtcaa agtccccaat ggacaacgcg tcgctcggga tcgtcggctg gatctggttt
      541 ctgcgggtgg tcgccgccgt caacgtggcg atctggctgc gggtcgcggt ggcgctgcaa
      601 cgggatcgcg cgctgcaggg cctcgagcgc cgccgctacc gccgtcgtca gctgattctc
      661 tcggcgctgt tcgtgggcgg gtgcgccttc cggtcgatct ttccccgggc cgacgtgcag
      721 cgcatctgcc tctacgacaa ctggctctcg gtcgtgctcg tcggacgcgc ggtggcgacg
      781 atcgccgagc tggcgttcat gacccaatgg gcgttgctgc tcggtgagtc ggccggcgac
      841 gggcagcgcg acgtcgccaa gatcgtttcc cgggtcctgg tcccaatcat cgcggtcgcc
      901 gagatctgct cctggtacgc ggtcgtgtcg acgaatttcc tcggcaacgc gatcgagcaa
      961 tcgatctgga cgttcgcttc ggtgctggtg atcgttggcc tgctgtcgcg cgaacgcggc
     1021 gcgcggcagc gcttcgtggg gatcgcgacg gccctcatcg gcgcgtatat cgtgttcatg
     1081 tcgatggtcg acgttccgat gtacctccgt cgctggcggg ccgacgagca gagccggcgc
     1141 ccgtatcttt ctttcgcgga gggggtccgc gacgtcgggt cccgccgcgt cgtgacgcgc
     1201 gcctgggagc cctggcgcga ggaagtgccc tggatgtcgc tctacttcag caccggcgtc
     1261 tgggtcagct tatggctaat tcgcgccgcc cccgcccgca agggttccgt gacactcggt
     1321 gacgagaacg tcacccaaag ttcgctttcc aaggcgcgaa gttgacggct aattgagcca
     1381 atgttgtttg gctcgtcaac gagcaaagga gagctcatga acggcttttt cgaggaattg
     1441 aaagagcaac gctgggacga ccaccgtttc taccatcaca gccgcatcaa ccagtcgctg
     1501 cacctggtga gcgcgatcag cttctgcgtc acctacgtgc ttttgttcaa gcacccggtg
     1561 gtcgcagcgc tgttcggttg gacgttcgcc atgtgcattc ggcaggtggg ccacttcttc
     1621 ttcgagccca agggctacga cgtggagaac caggccaccc acgaatacaa agagagggta
     1681 aaggtcgggt acaacctgcg ccgcaaggtg atcctgcaca gcctctgggc gctggtgccc
     1741 atcgcgctct attttgcgcc gacagcgttg ggggctttta cggtgccatg gacgaaccta
     1801 tcgacctatc tcgcgcactt ggcgcgcctc tggattggcc tggcggccgg cgcgctgctg
     1861 tttcggacca tccacctgtt cttcctgcgc ggcgtgaaga cggggctggt ctggttcacc
     1921 aagatcctca ccgacccgtt tcacgacatc tacctttacc accgcgcgcc gctctacctg
     1981 tggcgcggtg agttgatcga gcccgccaag cagtctgcct aatcgccctt gccgaaggct
     2041 gcggcgacca tctcccggag gaccctgcgc ttgatcgcct tgtcggtgag cgccgggtcc
     2101 gaccaccaga agccgtccgc ctgcatggcc cgcgcggccg acagaaactt gtcggccacg
     2161 gcgtcgaagt cggcgtcgct gtagttgaga ctgaagatga agcgtccggt ccccacccac
     2221 gagagcgcca acccctgagc acgcagatag aattgaagca tccagttgta tcggcccggc
     2281 tgcgtgtaga gaacggtcca gatcgtctgc aggttctcca ggcgcaccgg cagcccatcg
     2341 tcgacgaggc gtcggttgag ccgctcggcg cgaccgttcc aggtgtcgtc gaggttcctg
     2401 taa
//
LOCUS       DATCBJ010000410         2993 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_269627, whole genome shotgun sequence.
ACCESSION   DATCBJ010000410 DATCBJ010000000
VERSION     DATCBJ010000410.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2993)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2993)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2993
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_269627"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..525)
                     /locus_tag="VII50_08255"
     CDS             complement(<1..525)
                     /locus_tag="VII50_08255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5050879.1"
                     /translation="MTSPTDPRTHRVQETLRAQRIELPSWAFGNSGTRFKVFAQAGVP
                     RDPFEKIEDAATVNRLTGVAPKVALHIPWDRVDEFAKLRQHASDLGVELGTINANVFQ
                     DNDYMLGSVTNPNPTVRRKATDHLLECIDIMDQTGSRDLKLWFSDGTNYPGQDDVRDR
                     QDRLAESLAEVYARL"
     gene            complement(522..884)
                     /locus_tag="VII50_08260"
     CDS             complement(522..884)
                     /locus_tag="VII50_08260"
                     /inference="COORDINATES: protein motif:HMM:NF017174.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HEY5050880.1"
                     /translation="MRRYCFLLQVRPELLAEYRTRHAAVWPDMLQALHDSGWHRYSLF
                     ARDDGLLVGYVEAEDLTEAQEKMAATEVNGRWQESMSRFFAGLEGRRPDEGFLVLEEV
                     FNLDDQLARRAGTELSRP"
     gene            complement(1003..2136)
                     /locus_tag="VII50_08265"
     CDS             complement(1003..2136)
                     /locus_tag="VII50_08265"
                     /inference="COORDINATES: protein motif:HMM:NF024799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEY5050881.1"
                     /translation="MLALTALALVATACSSKKSSTPAASSTPAVAATTSAAAAPATSA
                     AAAPATSAAAAPASSAAAGVPANIKKGLVVYFIPKDTQNPYEVIADKGGEAALTELGG
                     KVIVSSGTSDTAAAQIPSIQAAIQAKADAIVIAGNDPSALCPSLAQAQAAGIKVVSFD
                     SDVTCPNHLFINQANTQQIGTSEVDLLAKEINSTGQIAILSAAATATNQNAWIGYMKQ
                     QLAKYPNMKLVSTVYGNDDPATSLTVLQGLLSAYPNLAGIISPTTVGISTAAQYLDTH
                     KSLLAHLTLTGLGLPSQMKTYVLDGTVKAFELWNPSNLGYLAGYAAAQAASGTDITTA
                     GGTFTAGKLGSYTVLPAASGTGPSVVLGPPTVFDASNVAQFNF"
     gene            complement(2258..>2993)
                     /locus_tag="VII50_08270"
     CDS             complement(2258..>2993)
                     /locus_tag="VII50_08270"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY5050882.1"
                     /translation="YFFQHGWPIALAIAAVLIVGAAGGALNGVLVTKLGLPSIAVTIG
                     TLTLFRGIAEIVLGTNSVGGFPQSLTKVGVLPIPHTRIAYSMGIFLVMAVVFGVVLHA
                     TPVGRSITAIGLQSEAARFSGIRVTRIKFLLYVLSGVVCAFAGVLWTMRFDTSRYDAG
                     TGLELTVVAAVLLGGVSIFGGRGTIFGVVVAIVVIGCIQEALTLMNVAAQVQNIVTGG
                     LLLVSVVVPNSPEALRRIRARAQRRQ"
BASE COUNT          525 a          893 c         1028 g          547 t
ORIGIN      
        1 aagccgcgcg tacacctccg cgagagactc ggccaaccga tcctggcggt cgcgcacgtc
       61 gtcttgtccg ggatagttcg tgccgtcgga gaaccacagc ttcaggtctc gcgagcccgt
      121 ctggtccatg atgtcgatgc actcgagcag atggtcggtc gccttgcgcc taacagttgg
      181 gttcgggttg gtcaccgagc cgagcatgta gtcgttgtct tggaacacgt tcgcattgat
      241 cgtgccgagc tcgacgccga gatcagacgc atgctgccgc agcttcgcaa actcgtcgac
      301 acggtcccac ggaatgtgca gcgcgacctt cggggcgacg ccggtcagtc ggttcacggt
      361 cgcggcgtcc tcgatctttt cgaacggatc gcggggaacg ccggcttgcg cgaatacctt
      421 gaagcgggtg cccgagttgc cgaacgccca tgacggcaac tcgatccgct gtgcccgcaa
      481 cgtctcctgg acgcggtgcg tgcgggggtc tgttggtgat gtcatggtcg ggatagctcc
      541 gtccctgctc gtcgtgccaa ctggtcatca aggttgaaga cttcctcgag gaccaggaat
      601 ccctcgtccg gacggcggcc ctcgaggccg gcgaagaacc tgctcatcga ctcctgccag
      661 cgcccgttca cctcggtcgc tgccattttc tcttgcgcct ccgtcagatc ctccgcctcg
      721 acgtagccga cgaggagacc gtcgtctctg gcaaacagcg aatagcgatg ccagccggag
      781 tcgtgcagtg cctgcaacat gtcgggccac accgccgcgt gtcgtgtgcg gtactcggcg
      841 aggagctccg gccgaacctg gagcaggaaa cagtagcggc gcatgggtgc ctcgttcgtc
      901 gcgtcattcg atggtgcgtt aggtggtgca tcgtcggtgt tcccgcgcgg cctgcgtgtt
      961 gcaggccgca cgggacacca cgtcagtccg ttaggcagtc gatcagaagt tgaactgcgc
     1021 gacgttggat gcgtcgaaca cggtgggcgg gccgagcacg accgaaggtc cggtgccact
     1081 cgcagccggc agcacggtgt agctaccgag cttgcctgcg gtgaaggtgc cgccagcggt
     1141 cgtgatgtcg gtgcccgaag cagcctgtgc agccgcgtac ccggcgaggt agccgaggtt
     1201 cgacgggttc cacagctcga acgccttcac ggtgccgtcc aggacgtagg tcttcatctg
     1261 cgacggcaag ccgaggccgg taagcgtcag gtgggcgagc aacgacttgt gcgtgtcgag
     1321 gtactgcgcg gcggtcgaga tgccgaccgt ggtcggcgaa atgatgccgg ccaggttcgg
     1381 gtaggccgag agcagaccct gcagaacagt gagggacgtc gcggggtcgt cgttgccgta
     1441 gaccgtcgag acgagcttca tgttcgggta cttcgcgagc tgctgcttca tgtagccgat
     1501 ccaggcgttc tggttcgtcg ctgtggcggc ggccgagagg atcgcgatct ggccggtgct
     1561 gttgatttcc ttggccagca agtcgacctc agaggtgccg atctgctggg tgttcgcctg
     1621 gttgatgaac aggtggttcg ggcaggtgac gtcggagtcg aacgagacga ccttgatgcc
     1681 tgcggcctga gcctgggcaa gcgacgggca cagcgccgac gggtcgttgc ccgcgatgac
     1741 gatggcgtcg gccttggcct ggatggctgc ctggatggaa ggaatctggg cggccgcggt
     1801 gtcggaggtg ccactactga cgatcacctt gccgccgagc tcggtcagtg cagcctcgcc
     1861 acccttgtca gcgatcacct cgtaagggtt ctgggtgtcc ttcgggatga agtagacgac
     1921 caggcccttc ttgatgttcg ccggcacacc ggcggccgca gagctggccg gggctgcggc
     1981 ggcagaggtc gccggagcgg ctgccgcaga ggtcgccggg gctgcagcgg cgctggtggt
     2041 agcagcaacc gccggtgttg atgacgcggc aggcgtcgat gacttcttcg aactgcacgc
     2101 cgtggcgacc aaggccaggg ccgtcagagc aagcacggcc gaggtccttc ggcttcgcgc
     2161 ggtaatgcgg atcatcttgt ttccttccaa tggtggttac tgtgccggga ctaacgggac
     2221 tgttcggtgc gttttcgaac tggtttggcg cggcgattca ctgccgtcgt tgcgcccgag
     2281 ctcgtatccg tcgcaatgcc tcgggactgt tggggacgac gacactgacg agaagcagac
     2341 ctccggtgac gatgttctgc acttgcgcgg caacgttcat gagggtgagt gcctcttgaa
     2401 tgcaaccgat gacgacgatc gcgaccacga caccgaaaat cgtgccgcga ccgccgaaga
     2461 tcgagactcc gccaaggagc acggcggcga cgacggtgag ctcgagcccg gtgccggcgt
     2521 cgtagcgaga cgtatcgaat cgcatggtcc agagaacgcc ggcaaatgcg cagacgacgc
     2581 cggaaaggac gtagagcagg aacttgatgc gggtcacgcg gataccggag aagcgcgcag
     2641 cttctgactg cagaccaatg gcggtgatcg aacgcccgac cggggtcgcg tgtaacacga
     2701 caccgaagac gaccgccatc acgaggaaga tgcccatcga gtacgcgatc ctggtatgcg
     2761 gaatcggtag gacgccgacc ttcgtcagtg actgcgggaa gccgccgact gagttggtgc
     2821 cgagcacgat ctctgctatg ccacgaaaga gcgtcagcgt gccgatcgtc acggcgatcg
     2881 agggcaaccc cagcttggtg acgagcacgc cgttcagcgc accaccggcg gcgccgacaa
     2941 ttaggaccgc cgcgattgcg agcgcgatcg gccagccgtg ctggaagaaa tat
//
LOCUS       DATCBJ010000411         3981 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_493810, whole genome shotgun sequence.
ACCESSION   DATCBJ010000411 DATCBJ010000000
VERSION     DATCBJ010000411.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 3981)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3981)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3981
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_493810"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1449)
                     /locus_tag="VII50_08275"
     CDS             complement(<1..1449)
                     /locus_tag="VII50_08275"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HEY5050883.1"
                     /translation="MATPQQTRHVFVTGGVASSLGKGLTASSLGNLLKARGLRVTMQK
                     LDPYLNVDPGTMNPFQHGEVFVTDDGAETDLDIGHYERFLDTDLHGSANVTTGQIYST
                     VIAKERRGEYLGDTVQVIPHITNEIKSRIRQMSGPDVDIVITEVGGTVGDIESLPFLE
                     AARQVRHEVGRDNVFFLHVSLVPYIGPSGELKTKPTQHSVAALRQVGIQPDAVVCRAD
                     RPIPQSVKRKISLMCDVDEEAVIAAVDAPSIYDIPKVLHAEGLDAYVVRRLGLPFRDV
                     DWTAWDDLLRRVHHPAHEVTIALVGKYIDLPDAYLSVTEALRAGGFANDARVNIRWVT
                     SDDCSTPEGAAKALLGVDGVVIPGGFGVRGIEGKLGAIRYARENLVPTLGLCLGLQCL
                     VIESARNVAGLSDANSAEFDAATPHPVIATMADQRDVIAGERDMGGTTRLGLYPADLL
                     AGSIVREMYGEARVNERHRHRYEVNNEYRGQLE"
     gene            complement(1508..3544)
                     /gene="steA"
                     /locus_tag="VII50_08280"
     CDS             complement(1508..3544)
                     /gene="steA"
                     /locus_tag="VII50_08280"
                     /inference="COORDINATES: protein motif:HMM:NF040608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative cytokinetic ring protein SteA"
                     /protein_id="HEY5050884.1"
                     /translation="MSAATTLLSATMGRVRLATLWRTRQPPLPGIIAMARVDRRTRQL
                     VKRLRPGDIAIIDHVDIDRLAAESLVECGVAAVVNAATSISGRYPNLGPEILVAHGIP
                     LLDGVGTGVLAEVRDGDIVRLCDGSLYAGDRVVAAGEQQTVETVQRAMADARAGLAVQ
                     LEAFAANTMEYLRRDRDLLLDGVGVPEIATDLAGKQVLVVVRGYNYREDLLALRRYIR
                     AYRPVLIGVDGGADVLLEGGYRPALVVGDVESLSDDALQCGAEIVVRRDPDGRAAGLE
                     RIQNLGLIAVEFTATGTSEDIALLLADAKGAALIATAGTHATLVEFLDKGRSGMASTF
                     LTRLRVGGKLVDAKSVNRLYRSRMSTSVLLWLAFVVALVVAVVSAGAVVRSHDLKSQR
                     AQVGQLQQQVDALQTHGATLQTEVDAGTGFAQAVEPLAVAGRLIGEQVVVIVAPNAPK
                     SVLDATVQSLADAGATLTGVIDVHARFVDPAQVTSLGELATSLGATAPSQNVPAQVSA
                     LLAGALVGQPPAGSTAGTLDAASTEILAGLAQGGFISVAQAPTQHARLAVIVAATPAA
                     PRASPTATETAARDALVELVAAFPAAGGTAVVAGASGSAADGGLLAALRAHASAGAGT
                     AAVSTVDDAEVAAGSIAVIMALQAEQAQPGGTAAVGHYGSGPGAQALLPAPPPS"
     gene            complement(3573..>3981)
                     /locus_tag="VII50_08285"
     CDS             complement(3573..>3981)
                     /locus_tag="VII50_08285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HEY5050885.1"
                     /translation="LAKGASGGELSRVMLAVEVVFAGADPVPTFVFDEVDAGVGGRAA
                     VEVGRRLARLARVAQVIVVTHLPQVAAFADRHLLVVKSDDGVVTRSGVVALDEAGRVN
                     ELSRMLAGLEGSTLAQGHAGELLAAASGFKQDL"
BASE COUNT          700 a         1373 c         1280 g          628 t
ORIGIN      
        1 ttcgagctgg ccacggtact cgttatttac ttcgtaccga tgccggtggc gctcgtttac
       61 gcgcgcctcg ccgtacatct cccgaacgat cgagccggcc aacaggtcgg cggggtaaag
      121 cccaagccgc gtcgtgccgc ccatgtcacg ctcaccggcg atcacgtcac gctggtcagc
      181 catcgtcgcg atgaccggat gcggcgttgc ggcatcgaat tctgcggagt tcgcatctga
      241 caaacccgcg acatttcgcg ccgactcgat gacgaggcac tgcaaaccaa ggcacaaacc
      301 cagcgtgggc accaaattct cgcgggcata gcgaatagcg cccagcttgc cctcgatccc
      361 ccgcacccca aacccacccg ggatcacgac accgtcgaca ccgagcaacg ccttcgctgc
      421 accttcgggt gtcgagcagt cgtcagaagt tacccaacga atattcaccc tggcatcgtt
      481 ggcaaatcca cccgcacgca atgcctcggt caccgagagg taggcgtcgg ggaggtcgat
      541 gtacttgccc accagggcga tcgtcacctc gtgtgccggg tggtgcacgc gacgcaagag
      601 atcgtcccat gcggtccagt cgacatcacg gaacggcagg ccgagtcggc gcacgacgta
      661 agcgtctagg ccctctgcat gcagcacctt cgggatgtcg tagatgctcg gtgcgtcgac
      721 cgcggcgata acagcctctt cgtcgacgtc gcacatcagg ctgatcttgc gtttgacgct
      781 ctgcgggatc ggccggtcag cgcggcacac gacggcgtcc ggctggatac cgacctggcg
      841 cagcgcagca accgagtgct gcgtcggctt cgtcttcagc tcgccactcg ggcctatgta
      901 cggaactaac gacacgtgca ggaagaacac gttgtcacga ccgacctcgt gccgcacttg
      961 tctcgccgct tcgaggaacg gcagggactc gatgtcgcca accgtgccac cgacctcggt
     1021 gatgacgatg tcgacgtcgg ggccgctcat ctgccggatc cgggacttga tctcgttcgt
     1081 gatatgcggg atcacctgga ccgtgtcgcc cagatattca ccccggcgct ctttcgcaat
     1141 caccgtcgag tagatctgac ccgtggtgac gttcgcggac ccgtgcaggt cggtgtcgag
     1201 gaaccgctcg tagtggccga tgtcgaggtc ggtttccgca ccgtcgtcgg tgacgaacac
     1261 ctcaccatgc tggaaaggat tcatcgtgcc ggggtcgacg ttcagatacg ggtcgagttt
     1321 ttgcatcgtc actcgcagcc cgcgcgcctt cagcagattg ccgaggctcg acgctgtcaa
     1381 gcccttgccc agtgacgagg cgacgccccc ggtaacgaac acgtgcctgg tctgctgcgg
     1441 cgttgccaat tcgtgactcc cgtggtctct ggcaggtgca ctgcccacgg gctctcaggg
     1501 taacagctta agaaggtggc ggggcaggca gcagggcctg tgcaccggga ccgctgccgt
     1561 aatgaccgac cgccgccgtg cctccgggtt gggcctgttc ggcctgcagg gccatgatca
     1621 ccgcaatgct gccagcggcc acctccgcgt cgtcgaccgt actcacggcg gctgtgccag
     1681 cgccagccga tgcatgggcg cgcagtgcgg cgagcagccc gccgtcggcc gcggacccgc
     1741 tggcgccggc aaccaccgcg gtgccccccg ctgcggggaa ggcggcgacg agttcgacga
     1801 gcgcgtcccg ggccgcggtc tcggttgcgg tcgggctggc ccgcggcgcg gccggggtgg
     1861 ctgcgacgat gactgcgaga cgggcgtgct gggtaggagc ctgcgcgacg gagatgaatc
     1921 cgccctgggc caacccggcg aggatctcgg tgctggccgc atcaagggtg cccgccgttg
     1981 agcccgccgg gggctgtccg acgagcgcgc ccgcgagcag cgccgacact tgagccggaa
     2041 cattctgact gggagcagtc gcgcccagtg aagtcgcgag ctcaccgagg ctggtgacct
     2101 gggccggatc gacgaagcgt gcgtgcacgt cgatgacccc ggtcagggtg gcgcccgcgt
     2161 cggccaagct ctgcacggtg gcatcgagca cgctcttcgg cgcgttggga gcgacgatga
     2221 cgacgacctg ctcgccgatg agccgaccag ccaccgccaa tggctcgacc gcctgcgcga
     2281 acccggtgcc ggcatcgacc tccgtctgca gcgtggcacc gtgtgtctgc agcgcgtcga
     2341 cctgttgttg cagctgcccg acctgcgctc gctgactctt caggtcatga cttctgacga
     2401 ccgcaccggc cgacacgacg gccacgacaa gcgccaccac gaacgcaagc caaagcagca
     2461 ctgacgtcga catgcggctg cggtagagcc ggttcacact cttcgcgtcg acaagcttgc
     2521 cgccaacccg cagccgggtc aggaacgtgc tcgccatccc ggagcgaccc ttgtcgagga
     2581 actccacgag ggtggcgtgg gtgccggcgg tggcgatcag ggcggcgccc ttagcgtcag
     2641 ccaacaacaa cgcgatgtcc tcgctcgtcc cggtcgcggt gaactcgacc gctatcaagc
     2701 cgaggttctg gatccgctca aggccggcag cacgtccatc gggatccctg cgtaccacga
     2761 tctcggcacc acactgcagg gcgtcatcgg agagggattc gacgtcgccg accaccaggg
     2821 ccggccggta gccgccctcg agaagaacgt cggcgccacc gtcgacgcca atgagtaccg
     2881 ggcgataggc gcggatgtag cgccgcaacg cgagcaggtc ttcgcggtag ttgtatccgc
     2941 gcaccacgac gagcacctgc ttgccggcaa ggtcggtcgc gatctcgggg acgcccacgc
     3001 cgtcaagcaa caggtcgcga tcgcggcgca ggtactccat ggtgttggcg gcgaacgcct
     3061 caagctgcac ggccaagcca gcacgcgcat cggccatcgc ccgctggacc gtctcgaccg
     3121 tctgctgctc ccccgcagcg accacccgat cgccagcgta caacgagccg tcgcagagcc
     3181 ggacgatgtc gccgtcacga acctcggcaa gcacgccagt tccgacacca tcaagcagcg
     3241 gtatcccgtg ggcgacaaga atctccggac cgaggttggg atagcggccg ctaatactcg
     3301 tcgcagcatt cacgactgcc gcgactccgc attcgaccag tgactccgcg gcgagacggt
     3361 cgatatcgac gtggtcgatg atggcaatgt cgccgggacg cagccgcttg acaagctgac
     3421 gggtccgccg gtccaccctg gccatcgcga tgattcctgg caacggaggc tgccgggtac
     3481 gccaaagagt cgcgagtctc acccggccca tcgtggcaga caacaacgtc gtcgccgcgc
     3541 tcatcgggcc cgcgccgagc aggtattagc gcctacaggt cctgcttgaa acccgacgcg
     3601 gcggccagca gttcgccggc gtggccttgg gccagggtgg atccctcgag accggccagc
     3661 attcgggaca gctcgttgac ccggccggcc tcgtcgaggg cgacgacgcc actgcgtgtc
     3721 accacgccgt cgtcggactt cacgaccaga aggtggcgat ccgcgaacgc cgcgacctgt
     3781 ggcaggtgcg tgacgacgat gacttgagcg acccgggcga ggcgcgccaa ccggcgtccg
     3841 acctcgactg cggcacgccc accgacgccc gcatcgactt cgtcgaagac aaacgtcggc
     3901 accggatcgg caccggcgaa cacgacctcg acggcgagca tgacgcggga caactcacca
     3961 ccactggccc ccttggccag c
//
LOCUS       DATCBJ010000412         3803 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_202618, whole genome shotgun sequence.
ACCESSION   DATCBJ010000412 DATCBJ010000000
VERSION     DATCBJ010000412.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 3803)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3803)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3803
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_202618"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..832)
                     /locus_tag="VII50_08290"
     CDS             complement(<1..832)
                     /locus_tag="VII50_08290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HEY5050886.1"
                     /translation="MRLSVIGTGYLGATHAASMAELGHKVVGVDIDPIKIDRLAAGHV
                     PFYEPGLPELLSKHVTSGALRFTTSIQTAAAFADLHFVCVGTPQKQGEYAADLTLVDA
                     AFLALREWVRPGAVVVGKSTVPVGTATRLAALLRDAGVELVWNPEFLREGFAVEDTLR
                     PDRIVIGATNDAARELLETAYAPILAAGTPVVRTDFATAELVKVAANAFLATKISFIN
                     AVAEVCEVAGADVTQLAEAIGHDDRIGHKFLRAGLGFGGGCLPKDIRAFMARAGELGV
                     D"
     gene            complement(829..3234)
                     /gene="rfbD"
                     /locus_tag="VII50_08295"
     CDS             complement(829..3234)
                     /gene="rfbD"
                     /locus_tag="VII50_08295"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: protein motif:HMM:TIGR01214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008831 - dTDP-4-dehydrorhamnose reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HEY5050887.1"
                     /translation="MAPRGWNTGTQDSLIEAAQFVQVLQHRQGITIACLEEVAMRRGI
                     ITAEQVLALGDEMGKSGYAGYLSSVAIDFLAPSSVFSWEERLRDEHGCAAGHHADGRR
                     AHRDRRAAADHPEGGHRRARHGARVFRASSYGAAGVGADGWRQVNLTWTQRGALRRLH
                     GEATGKLVGVACGEAFGVYLDARRGSPTFGLVVTERLTPGVQVFVPAGVCNGFQAVSD
                     PGCQYLYCFGAEWQPGMPGVAVNPLDRELRIAWPIAPEPGNPAMVSAKDASAHASPTC
                     AVAVRSEQHAATRHRRPRLHRRQLRSLHARHRPHTNIMVLDAMTYAANESSLDVVRHR
                     IRVVRGDISDVPLVNDLVGHSEFVVHFAAESHNDNSLRNPSPFVHTNLVGTFTILEAV
                     RRHGGRLHHVSTDEVYGDLALDDPAKFTPDTPYNPSSPYSSTKAGSDLLVRAWCRSFG
                     LSATISNCSNNYGPHQHIEKFIPRQITNMLSGRRPKLYSTGANVRDWIHVDDHNAAVW
                     TLGHHVSDQLRASGADVVAVTATGLDITDRAAVDETIAAYRPDVVLNAAAYTAVDAAE
                     TDEQPADAVNQHGPRLLAEAVRRCGGRLLHVSTDYVFDGTASRPYEIDDPVGPQGAYG
                     QTKLAGERAVLGVLPDRAHVVRTAWVYGGPGPDFVDTMLRLERERETLDVVADQIGSP
                     TWVADLAAALIELGASAVPADVLHYVNGGQASWFRPGPRGVLACRRQTVADASGRYRC
                     LPASGPPSRVVGAVYAGVDGGRVASAAPVAGCARRRTGGRTEQGNVGGRSLTLRNLVR
                     EKK"
     gene            3385..3615
                     /locus_tag="VII50_08300"
     CDS             3385..3615
                     /locus_tag="VII50_08300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5050888.1"
                     /translation="MTLVAALEREDAASWRGMVERHRESLEELTSAADGVIAVTGRDN
                     PGRPEVGLALLMWAAELASAVRPDAAHDQREI"
     gene            3605..>3803
                     /locus_tag="VII50_08305"
     CDS             3605..>3803
                     /locus_tag="VII50_08305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5050889.1"
                     /translation="MKSDFGHFLDLILLAGRVTCGFVLCDCRSCALGAAVLWSGVPVS
                     ADGQGIVGRDGRADRALIAARV"
BASE COUNT          614 a         1285 c         1298 g          606 t
ORIGIN      
        1 ggtcgacgcc gagttcgccg gcgcgcgcca tgaacgcccg gatgtccttg ggcagacagc
       61 cgcccccgaa gccgaggccg gcgcgcagga acttgtgccc gatgcggtcg tcgtgcccga
      121 ttgcctcagc gagctgcgta acgtctgcgc cggccacctc gcacacctcg gccaccgcgt
      181 tgatgaacga gatcttggtg gcaagaaaag cgttcgcggc caccttgacg agctcggcag
      241 tggcgaagtc ggtgcgcacg acgggcgtgc cggccgcgag gatcggtgcg taggcagtct
      301 caagaagctc ccgagcggcg tcgttcgtcg cgccgatgac gatgcggtcc gggcgcaatg
      361 tgtcctcgac cgcgaacccc tcgcgcagga actcagggtt ccagaccagc tcgactcccg
      421 cgtcgcgaag cagcgccgcc agccgcgtgg cggtgccaac cggcacagtc gacttgccca
      481 cgacaacagc acccggccga acccactcac gcaacgcgag aaacgcggcg tcgacaagcg
      541 ttagatcggc ggcgtactca ccctgcttct gcggggttcc gacgcatacg aagtgcaaat
      601 cggcgaaggc cgctgccgtc tggatcgacg tcgtgaagcg cagcgcgccc gacgtgacgt
      661 gcttgctgag cagttccggc aagcctggct cgtagaaggg cacgtggccg gccgccagcc
      721 ggtcgatctt gatcgggtcg atgtcaacac cgaccacctt gtgccccagc tcggccatcg
      781 acgccgcgtg cgtcgcgccg agatagcccg tgccgattac cgaaagcctc attttttctc
      841 ccgcaccaaa ttgcgcaatg tcaacgaacg gcccccgaca ttgccctgct cggtgcgccc
      901 tccagtgcgg cggcgagcgc atcctgccac cggcgcggcg ctcgcaaccc ggccgccatc
      961 cacgcccgcg tagacagcac cgaccacgcg ggacggcggg ccggacgcgg gaaggcagcg
     1021 gtatcgaccg gacgcatccg cgacggtttg gcgcctacaa gccagaacgc ctcgcgggcc
     1081 aggtcgaaac cacgacgcct gcccgccatt aacgtagtgc aggacgtcgg cgggcaccgc
     1141 gcttgcgccg agctcgatca gcgctgccgc aagatccgcg acccacgtcg ggctgccgat
     1201 ttggtcagcg acgacgtcga gcgtctcgcg ctcgcgttcc aaacgcagca tggtgtccac
     1261 gaagtcgggg cccggcccgc cgtagaccca cgcggtccgt acgacgtgcg cgcgatcggg
     1321 cagcacgccg agcacggccc gctcgccggc gagcttcgtc tgcccgtacg cgccctgcgg
     1381 accgaccgga tcgtcgatct cgtacggacg ggacgcggtt ccgtcaaaga cgtaatcggt
     1441 ggacacgtgc agcagccgcc cgccgcagcg acgtactgct tcggccagca gccgcggccc
     1501 atgttggttg accgcatcgg ccggctgctc gtccgtctcg gccgcgtcga ccgccgtgta
     1561 ggcggctgcg ttgagcacga catcagggcg gtatgcggcg attgtttcgt cgaccgcggc
     1621 gcgatcggtg atgtcgaggc ccgtggcggt gaccgcgacg acgtccgcgc cggatgcgcg
     1681 cagttggtca gagacgtggt ggccgagcgt ccataccgcc gcgttgtgat cgtccacgtg
     1741 gatccagtcg cgcacgttcg cgcctgtgct gtacaacttc ggccgccgcc cggagagcat
     1801 gttggtgatc tgccgcggga tgaacttctc gatgtgctgg tgcgggccgt agttgttcga
     1861 acagttcgag atcgtcgcac tgagcccgaa cgaccggcac cacgcgcgca ccagcaagtc
     1921 cgaaccagcc ttggtcgagc tgtacgggct cgacgggttg tatggcgtgt caggcgtgaa
     1981 cttggccggg tcgtcgagcg cgaggtcgcc gtacacctcg tcggtcgaca cgtgatgcaa
     2041 gcgacctcca tgccgccgta ccgcctcgag gatcgtgaac gtgccgacca ggttggtgtg
     2101 gacgaacggc gacggattgc gcagcgagtt gtcgttatgc gactcggcgg cgaaatggac
     2161 gacgaactcc gagtgcccga cgaggtcgtt cacgagtggg acgtcggaga tgtcgccacg
     2221 gaccacgcgg attcggtggc gtactacgtc caggctcgac tcgttggctg cgtaggtcat
     2281 cgcgtctagc accatgatat tggtatgcgg ccggtggcga gcgtgtagtg aacgaagttg
     2341 gcgccgatga agccgaggcc gccggtgacg agtagccgca tgttgttcac tcctaaccgc
     2401 gacagcacag gtcggcgaag cgtgggcgga cgcgtccttc gccgacacca tcgcggggtt
     2461 ccctggctcg ggtgcgatcg gccaggcaat gcgcagctcg cggtcgagtg ggttgacggc
     2521 gaccccgggc atgcccggct gccactccgc gccgaagcag tacaggtact ggcacccggg
     2581 gtcggacacc gcctggaagc cgttgcagac tccggccggc acgaacacct gcacgcccgg
     2641 cgtcagccgc tctgtaacga ccagaccgaa ggtcggcgag ccgcgccgcg cgtccaggta
     2701 gacgccgaac gcctcgccgc acgcaacgcc gaccagcttg ccggtcgcct cgccgtgcag
     2761 cctgcggagc gcgccgcgct gcgtccaggt gaggttgacc tgccgccagc cgtcggcacc
     2821 gacaccggct gccccgtagc tgctcgctcg gaaaactcgc gcaccgtgcc gcgctcgtcg
     2881 gtgaccacct tcggggtgat ccgcagcagc ccgtcgatcg cggtgggctc gacggccatc
     2941 ggcatgatgt ccggccgcac aaccgtgctc gtcacgaagc cgctcctccc acgagaacac
     3001 gctgctcgga gcaaggaaat cgatggccac actgctcagg tacccggcgt agcccgactt
     3061 gcccatctcg tcgccgagcg cgagcacctg ttcggctgtg atgattccgc ggcgcatcgc
     3121 cacctcctcc aggcacgcga tcgtgatgcc ctgccggtgc tgcaggacct gcacgaactg
     3181 cgccgcctcg atcaacgagt cctgcgtgcc ggtgttccag ccacgcggtg ccacgaccca
     3241 ggtcgagcag ccatcaacta cgaactagac gcccaggcag cgggcctcga ttacgatcag
     3301 atccggcctt tgcgagaacg tgaccgcatg tccccccgct cccggtggtc acacgacggt
     3361 tagtcgcgcc gcttcaccga gccggtgacg ctggtggcgg cgctggagcg ggaggacgcc
     3421 gcctcgtggc gtgggatggt ggagcggcac cgggagagcc tggaggagct gacgagtgcg
     3481 gcagacggag tgatcgcggt aactggccgg gataacccgg ggcggccgga ggtggggctg
     3541 gcgctgctga tgtgggctgc tgagctggcg tcggcggtgc ggccggatgc cgcccacgac
     3601 cagcgtgaaa tctgacttcg gccactttct ggacttgatc ttgcttgcgg gccgggtgac
     3661 ctgcgggttt gttctgtgtg attgccgaag ttgcgcactt ggcgcggccg tattgtggtc
     3721 gggtgtcccc gtatctgcgg acggtcaagg catcgtcggg cgcgacggcc gtgcagatcg
     3781 tgcactcatc gcggcgcggg tcg
//
LOCUS       DATCBJ010000413         2385 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_187685, whole genome shotgun sequence.
ACCESSION   DATCBJ010000413 DATCBJ010000000
VERSION     DATCBJ010000413.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2385)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2385)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2385
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_187685"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..793)
                     /locus_tag="VII50_08310"
     CDS             complement(<1..793)
                     /locus_tag="VII50_08310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009083793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEY5050890.1"
                     /translation="MKVDRVLPTSEATELLELVGDIARDNLAPRAALAEREGSFPRDA
                     FVTLGASGLLGLPYPEEHGGAAQPYEVYLQVIEELSRAWLTVGLGISVHTLSCFPLAR
                     FGTDAQRDRWLPDMLGGALLGAYCLSEPDSGSDAGALRTRATRVDNRYQLAGTKAWIT
                     HAGQADFYTVMARTSDERSRGISCFLIAGETAGVRAGTPEHKMGLQGSPTGQLHLTDV
                     DVDADRLIGAEGQGFTIALAALDTGRLGIAACAVGLAQSALDVALD"
     gene            843..1793
                     /locus_tag="VII50_08315"
     CDS             843..1793
                     /locus_tag="VII50_08315"
                     /inference="COORDINATES: protein motif:HMM:NF014759.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exonuclease"
                     /protein_id="HEY5050891.1"
                     /translation="MTPSPAAPSAGLLLLDTPSLYFRAFYGIPESVTAPDGSPVNAIR
                     GLLDFISFFVERYRPSHVVACMDADWRPAFRVDLLPTYKAHRVAPTGGETVPDALTPQ
                     VVVIEQVLDAIGLCRLGVPGFEADDVIATLAVRATGPVDVVTGDRDLFQLVDDAKPVR
                     IVYTARGVGKAELIDEAAVTAKYGIPGRAYADFAVLRGDPSDGLPGVAGIGDKTAAAL
                     VSRFGSLVSMLSALESDSDAGFPAGTRTKLRASLGYLESARVVVATRTDVPIGELDDM
                     LPRRIHDEQGLLDLIQRYGLGTPAARLLTALNISPAAAAT"
     gene            complement(1790..>2385)
                     /locus_tag="VII50_08320"
     CDS             complement(1790..>2385)
                     /locus_tag="VII50_08320"
                     /inference="COORDINATES: protein motif:HMM:NF019758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RNA helicase"
                     /protein_id="HEY5050892.1"
                     /translation="RRVENRTNSIARVFDRVCDLLDHLGYLEGEKVTDAGRRLARLYG
                     ELDLLAAECLRGDVWRDLTPPELAACVSALVFEVREPTTAPPRLPRGKVPDVIDDAMK
                     LWGQLARAERDRRLDFLREPAPGFAWAAFRWASGASLDSVLTDNAMSAGDFVRWIRQL
                     LDLLGQIAQAADDDIRPVAERAMTALRRGVVAYSSIG"
BASE COUNT          381 a          816 c          789 g          399 t
ORIGIN      
        1 agtcgagggc gacgtcgagt gccgactggg ccaacccgac agcacaggcg gcaatgccca
       61 aacggcccgt gtcgagcgct gccagtgcaa tcgtgaaccc ctggccctcg gcgccgatca
      121 gccggtcggc gtcgacgtcg acatcagtga ggtgcagctg gccagtcggg gaaccctgta
      181 gacccatctt gtgttcggga gtacccgccc gcacacccgc cgtctccccc gcgatcagaa
      241 aacagctgat gccacgcgac cgctcgtcgg aggtgcgggc catcaccgtg tagaagtcgg
      301 cctgccccgc gtgggtgatc cacgccttcg taccggcgag ttggtatcgg ttgtcgaccc
      361 gcgtcgcgcg cgtgcgcaac gcaccggcgt ccgacccgga atctggttct gacaggcagt
      421 acgcgccgag cagcgcgccg cccaacatgt cgggaagcca gcggtcgcgc tgtgcgtcgg
      481 tgccgaagcg ggcgagcggg aagcacgaca aggtgtggac gctgataccg aggccgaccg
      541 tgagccacgc cctgctgagc tcttcgatga cctggagata aacctcgtac ggctgggccg
      601 cgccaccgtg ttcctccggg tacggcaacc cgaggaggcc gctcgccccg agcgtcacga
      661 aggcgtcgcg cggaaagctg ccttcgcgtt cggccagcgc tgcgcgcggt gcaaggttgt
      721 cacgggcgat gtcgccgacc agctcgagca gctcggtcgc ctcggacgtg ggtagaaccc
      781 ggtccacctt cattcgccga tcctgtcatg cgtcgccgtc gcggtcgtgc caagctcttg
      841 gggtgacgcc gtcgcctgca gcgccatccg caggcttgct ccttctcgat acgccctcgc
      901 tgtattttcg cgcgttctac ggcattcccg aatccgtcac cgccccggac ggatcgccgg
      961 tcaacgccat caggggcctt ctcgacttca tcagtttctt cgtcgagcgc taccgcccgt
     1021 cgcatgtggt ggcgtgcatg gacgccgatt ggcggccggc atttcgggtc gacctgctgc
     1081 ccacgtacaa ggcgcatcgg gttgcaccga caggagggga aaccgtcccc gatgcgctga
     1141 cgccgcaggt cgtggtcatc gagcaggtcc tcgacgccat cgggttgtgc cgtcttggcg
     1201 tccccggctt cgaggccgat gacgtcatcg ccaccttggc ggtgcgcgcg accggacccg
     1261 tcgacgtcgt caccggcgat cgcgacttgt tccagctggt cgacgacgcg aaacccgtgc
     1321 gcattgtcta cacggcccgt ggtgtcggga aagccgaact catcgacgag gctgcggtca
     1381 ctgcgaagta cgggattccc ggccgcgcat acgccgattt tgccgtcctg cgaggcgatc
     1441 cgagcgatgg cttgcccggc gttgcgggta tcggcgacaa gaccgcagcg gctctggtgt
     1501 cgcggttcgg gtctctcgtg tcgatgctgt cagcgcttga gtccgatagc gatgcgggct
     1561 ttcccgccgg gacccgcacg aagctgcggg cgtcacttgg ctacctcgag tccgcacgtg
     1621 tcgtggtggc gacccgaacc gacgtgccga ttggtgaact cgacgacatg ttgccgcgaa
     1681 ggatccacga cgagcagggt ttgctcgatc tgatccagcg ctacggactg ggaacgccgg
     1741 cggcacggct gctcacggcg ctgaatatca gtccggcggc ggccgcgacc taaccgatcg
     1801 aggagtaggc caccactccc ctgcgcaacg ccgtcatcgc gcgttccgca accggccgga
     1861 tgtcgtcgtc agcggcctgg gcaatctggc ccagcaaatc gagcagttgc cggatccacc
     1921 ggacaaagtc gccggccgac atcgcgttgt cggtcagcac cgaatcaagg cttgcgcccg
     1981 aggcccagcg aaatgcggcc catgcgaaac ccggcgccgg ctctcgcaag aagtccaacc
     2041 ggcggtcgcg ctcggcgcgg gcgagttgcc cccacagctt catcgcgtcg tcgatcacgt
     2101 cgggcacctt gccgcgcggc aaccggggcg gcgcggtcgt cggctcgcgg acctcgaata
     2161 ccagcgcgga cacgcacgcc gcgagttcgg gcggcgtcag atcccgccag acgtcgcctc
     2221 gcaagcattc ggcggcgagc aagtcgagct cgccgtagag gcgcgccaat cggcgtccgg
     2281 catcggtgac cttttcgcct tcgagatagc cgagatggtc gagcaggtca cacacgcggt
     2341 cgaagactcg cgcgatcgag ttggtccgat tctcgacccg gcgcc
//
LOCUS       DATCBJ010000414         6981 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_33542, whole genome shotgun sequence.
ACCESSION   DATCBJ010000414 DATCBJ010000000
VERSION     DATCBJ010000414.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 6981)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6981)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6981
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_33542"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..271)
                     /locus_tag="VII50_08325"
     CDS             complement(<1..271)
                     /locus_tag="VII50_08325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5050893.1"
                     /translation="MVFIRFPLKVRMPAVAPVHGWIGATLPPKVAVTIELPEPDPAWI
                     EAAIPVDGLVVMTVHVVPDGQLVAAPATLNELLVAAVSPVAVAVSV"
     gene            261..515
                     /locus_tag="VII50_08330"
     CDS             261..515
                     /locus_tag="VII50_08330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5050894.1"
                     /translation="MKTITLTIPIKQGSLVFCYGQPTPFLDIFLHKTTYFSTVNHEYE
                     GLLPFCFKNFTGPCVKSISATKTTETVVILSGTADPRVMR"
     gene            complement(505..4386)
                     /locus_tag="VII50_08335"
     CDS             complement(505..4386)
                     /locus_tag="VII50_08335"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEY5050895.1"
                     /translation="MSTSNADRSPNHSVHLEPFVSELARTWLNETPEVSARQLVGTLV
                     FADISGFTRLTERLAARGRFGAEEMSDHLDVVLSELLTAAYDHGGWLVKWGGDALLLM
                     FDTADHALRACDAAAAMQSRMRRVGTLETSVGRVRLRMSIGIHTGTFSFHFLGQRHRE
                     LLITGEAATVTAALEAAAEAGDILVSPMTATLLPDACRGVAKGPGILLAHPPRCEVID
                     GVAQSASAPVTGLIPELVLDHLVSGGGIGEHRQVTVAFLEFSGLSRLRLESGDAAVDD
                     ALRHLVDVAQESCHRNLVSFHETDISADGGKIMLVAGAPRGLEDPAEAMLCALRQIFE
                     DPGPLSLRAGVTAGRAFTGCVGPARRRSYSVKGDVVNLAARIMGKTPTGQIWALPAVA
                     ESSRTQFDLGDVPSFSVKGKVAPVVVRSVGLPLARIDVHGELPLIGRADEMNLLDAAL
                     VRAREGAGEHVDLLGAAGIGKTRVLAELKRMAWDMGVLAAAAEPYRASSPYALAAPLL
                     LEAIGAGEVATAALPEWLDQWCSRHAPELTNWLPLLAPMLGISIAETAQTRDIAPEFR
                     VERIHALVLDMLLVAISGPTLLVVDDMQFADEASDALLRHIARSAAGQPWLVVLAGRG
                     DEPRSDSDGSTTMVLDPLGPVDSEIFIYADTDDAPLAPHIAGAVVARGGGNPLFLRHL
                     AATARTARGTLDLPDTIETVVAARIDRLAPAAREVLRAAAVVGMSVDQELLGELLDEQ
                     PARAASVLEYLGEFLSSDGGELRFRQAVIRDTAYEGLAFRRRATLHGRLAQLLTDRHR
                     DDPESIDAILSLHYFEAGDHEPALRASRAAADRAADAHANTEAAVLYRRAIAAADRLR
                     ADDVAVRADLFERLGDVQVRLGEYDRGDLSHSAAARLLRTDPMVVARIGLKRARSAAR
                     MGAYDASLRRLRRVAEALEGVSGPVAADLRVEMQMNIGFTQFQQGRLAAARVSLQHAV
                     EHGDARRTPEVVADALAILDIVEVNLGLPVDGRRARRALRLHERRGDLAGQARVLTQI
                     GYRAYFDGRWNDAVGFYTEARDLVERLGDSSNVAVANGNIAEILLDQGRIEEAEGVLR
                     EAIRVWRSSGSENDVAFGRTLLGRVLARQGRYDEADVMLQQARDRFVEQGAKIDVVDA
                     DVFQAECLLLRGDHAAALDLAEASLLAAGRLSEQPAQAPLLYRVVGACHDALGNESDA
                     ELAYEHALTVARQRGAAHEIAFTVAAMAQRRRKAGQLLDEALLAEVRPLQRRLGLLLD
                     LTDVEAAPESSTDTSTVPVERPAFAVVPVTA"
     gene            complement(4473..6116)
                     /locus_tag="VII50_08340"
     CDS             complement(4473..6116)
                     /locus_tag="VII50_08340"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HEY5050896.1"
                     /translation="MRAEARLRTSLRRLRLDVALALAAIANRAYSVALVVYVYNATHS
                     SAWVAAAAAARYLPGIVTSVFAAPLLDRFPPRRLLVVSDICCAIAMAAMATAILVNAS
                     PAVAIALAAVVRIAASGQAPAAALLLPIVAGGGDLSRVASRQAATDKLLLLAGPAVGG
                     LMLLVISPAVEMLALAVLVAIAAVVSLGLPSPGQLRLLTPDPKAAGRTRKMRAHTTSG
                     IGIFVALMGLSGLVYGTDTVLLAVLATTRLHLGDAGYGELFAGLGTGGLLAAPVVNRI
                     VREHRLAGWLVLCMVVYCVPSVLIAHSHSSVAVVAFEALRGAGSLAVDVVALTELQRF
                     VLPQGLPLLTARLTSVVFGAVAIGALATPLSLHAFGTTGTLTILGVLPPIVVIGVYPV
                     LRRSDTASTSRFEELEPRIVVLQHLGLLQASSRPVLERLAADISELGISAGTTIIAEH
                     DVADAFYVVRAGELEVLVGDRHVNDLHAGDWFGEIGLLEGVPRTATVRTVVPSVLYRI
                     GGEAFLEAFAQLPPSPSLLDSVAARLAVSRLDAAASAQN"
     gene            6283..6684
                     /locus_tag="VII50_08345"
     CDS             6283..6684
                     /locus_tag="VII50_08345"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HEY5050897.1"
                     /translation="MSAVNPSELSRIALFAGLDQQDQRVVASWLDVGEFDPGYKLTHE
                     GASGYAFFVLHAGAANVIVGGNVVRTLGPGDFFGELSMLGDGRQTATVTITTPSTVWT
                     MFGTRFRELQQQHPAIAAAIEKVAAERVSPQ"
     gene            6782..>6981
                     /locus_tag="VII50_08350"
     CDS             6782..>6981
                     /locus_tag="VII50_08350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5050898.1"
                     /translation="MDATADTLQKALRINLDPRWYGTIAEIGAGQEVARWFFRAGGAA
                     GTVAKSISAYDMAVSDAVYGKSG"
BASE COUNT         1293 a         2372 c         2270 g         1046 t
ORIGIN      
        1 acacgctcac ggcaacggct accgggctaa cagcagcgac gagtaactcg ttcaacgtcg
       61 cgggtgccgc gacgagctgc ccatccggca cgacgtgcac cgtcatgacg acgagcccgt
      121 cgacggggat cgctgcttcg atccaggcag ggtcgggttc gggcaactcg atcgtgacgg
      181 cgaccttcgg cggcaacgtc gcgcctatcc acccgtgcac tggtgcgaca gcgggcatcc
      241 tgaccttcag cggaaaccgg atgaagacca tcacgctcac gattccgatc aagcaagggt
      301 cgctggtgtt ctgctacggc cagccgacgc cgttcctcga catcttcttg cacaagacca
      361 cgtacttcag cacggtgaat cacgagtacg agggactgct tccgttctgc ttcaagaact
      421 tcaccgggcc gtgcgtgaag tcgatatccg cgacgaagac aaccgagaca gtggtcattc
      481 tcagcgggac ggccgacccg cgcgttatgc ggtaacgggt accacggcga acgccggcct
      541 ttcgacaggc acggtcgatg tgtcggtcga cgactcggga gcggcttcaa cgtcggtgag
      601 gtcaagtagc agaccgagcc ggcgttgaag cgggcgcacc tcggcgagca acgcttcatc
      661 gaggagctgc ccagccttgc gccgccgctg cgccatggca gcaacggtga acgcgatctc
      721 gtgcgcggca ccacgctggc gggccacagt cagcgcgtgc tcgtaggcca gttcggcgtc
      781 cgactcgttg ccgagcgcgt cgtgacacgc tcccaccact ctgtacagca gcggtgcctg
      841 cgccggttgt tctgagagcc ggccggcggc aagcaatgac gcctcggcca gatccagcgc
      901 cgcggcatga tccccgcgca agaggagaca ttcggcctga aacacgtcgg cgtcgaccac
      961 gtcgatcttt gcgccctgct cgacaaagcg atcgcgggcc tgctgcagca tgacgtcagc
     1021 ctcgtcgtaa cgcccttgtc gagcgagcac ccgcccaagc agcgtgcggc cgaatgcgac
     1081 gtcgttttcc gaacccgacg atcgccatac gcggatcgct tcgcgcagca ctccttcagc
     1141 ttcctcgatg cgaccctgat cgaggaggat ctccgcgatg ttgccgttcg ctacggccac
     1201 gttcgacgag tcacccaacc gctcaaccag gtccctggct tcggtgtaga aaccgaccgc
     1261 gtcgttccag cgcccatcga agtacgcgcg atatccgatc tgcgtcagca cccgagcctg
     1321 tcccgctaag tcgccacgcc gttcgtgcag gcggagagct ctgcgggccc ggcgcccgtc
     1381 gaccggaagc cccaggttca cctcaacaat gtcgagaatt gcgagcgcat cggccaccac
     1441 ctctggcgtg cgccgagcgt ccccatgctc gaccgcgtgc tggaggctga ctcgagccgc
     1501 ggcgagccgc ccttgctgga actgggtgaa gccgatgttc atctgcatct ccacccgcag
     1561 gtccgctgcg acgggccccg agacaccctc caacgcctca gccacccgcc tgagccgacg
     1621 cagggatgcg tcgtacgcgc ccatgcgtgc ggcgctacga gctcgcttca gcccgatcct
     1681 ggcgacaacc atcggatcgg tgcgcagcag ccttgcagcg gcggagtgcg agaggtcacc
     1741 acggtcatac tcgccgagtc gcacctgcac gtcgcccaac cgttcgaaga ggtccgcgcg
     1801 cacagcgacg tcgtcagctc gcagccggtc agcagcggcg atcgcccggc ggtaaagcac
     1861 agcggcttcg gtattggcat gggcgtcggc agctcgatct gcggccgctc ggctggcgcg
     1921 tagcgccggc tcgtggtcgc cggcctcgaa gtagtgcaaa gacaggatcg cgtcaatcga
     1981 ttcggggtcg tcccggtggc gatccgtcaa cagctgcgcg aggcgaccgt gtaacgtggc
     2041 ccgccgccga aacgcgagtc cttcgtatgc agtgtctctg ataaccgcct gccggaacct
     2101 gagctccccg ccgtccgaag aaagaaactc accgaggtac tccagaacgc tcgctgccct
     2161 cgcgggttgc tcgtcgagca actcgccgag gagctcctgg tcgaccgaca tgcccacgac
     2221 ggccgcagcg cgcaggacct cgcgggccgc aggggcgagc cgatcgatgc gcgcggcgac
     2281 aactgtctcg atggtgtcag gaaggtcgag cgtgccgcgg gccgtccgtg cggtcgcggc
     2341 gaggtggcgg aggaacagcg ggttgccgcc acctcgcgcg acgacggccc cagcgatatg
     2401 cggggcgagt ggtgcgtcat cggtgtccgc gtagatgaag atctccgagt ccaccggtcc
     2461 gagcggatct agcaccatcg tggtggaccc gtcggagtcg gaccgaggct catcgccccg
     2521 acccgccagc acaacgagcc agggctgacc ggccgcgctc cgggcaatgt ggcgcagcaa
     2581 tgcgtccgat gcctcgtcgg cgaactgcat gtcgtcgacg acgagcagcg tcggaccgga
     2641 gatcgcgacg agcagcatgt caagcaccag cgcgtggatc cgttcgaccc gaaactcggg
     2701 cgcgatgtcg cgggtttggg cggtctcggc gatcgagatg ccgagcattg gcgcgagcag
     2761 cggcagccaa ttcgtcagct cgggcgcatg ccgcgagcac cattggtcga gccattccgg
     2821 caacgcggcc gtggcgacct cgcccgcacc gatcgcttcg agcagcagcg gcgccgcgag
     2881 agcatacgga gaagacgcgc ggtacggttc ggcggccgca gcaaggaccc ccatgtccca
     2941 agccatccgc ttgagctcag cgagcacccg ggtcttcccg atgcctgcgg cgccgaggag
     3001 atctacgtgc tcccctgcgc cctcgcgtgc ccgcaccaac gctgcgtcga ggaggttcat
     3061 ctcgtcggcc cggccgatca gcggcagctc gccgtggacg tcgatacgag caagcggtag
     3121 cccgacagac cgcaccacaa ccggcgctac cttgcccttg accgagaaac tcggtacatc
     3181 accgagatcg aactgagttc gggaggactc agcaactgcg ggtaacgccc agatctgacc
     3241 ggtcggcgtc ttacccatga tgcgcgccgc gagattcacc acgtcgccct tcacggagta
     3301 cgaccgacgc cgcgccggac caacgcaccc ggtgaacgca cgccccgcgg tcacaccggc
     3361 gcgaagtgac aacggaccag ggtcctcgaa gatctgtcga agggcacaca acatcgcttc
     3421 tgccggatct tcaagaccgc gaggcgcacc tgccacgagc atgatcttgc cgccgtcagc
     3481 agagatatcg gtctcgtgga atgagacgag gttgcgatgg catgactcct gggcgacatc
     3541 gacaagatgg cgcagcgcgt cgtcgacggc cgcatcacct gactccagcc gcagtctcga
     3601 caagccggag aactctagga acgcgacggt cacctggcgg tgctcgccga tgccaccgcc
     3661 gctgacgagg tggtcgagca ccaactcagg gatcagcccg gtgactgggg cggacgccga
     3721 ctgtgcgacg ccgtcgatca cctcgcaccg cggcgggtgg gcaagcaaga ttcccgggcc
     3781 cttggcgacg ccgcggcacg cgtcgggcag gagtgtcgcg gtcatcgggc tgaccaggat
     3841 gtcgccggct tcggccgccg cctccaaggc cgcagtcacc gttgccgcct cgccggtgat
     3901 gagcagttca cgatgccgct ggccgaggaa atgaaacgag aatgtgcccg tgtgaatccc
     3961 gatcgacatc cttaagcgaa cccggccgac tgacgtctcg agcgtcccga cgcgccgcat
     4021 ccgcgactgc atcgccgccg ccgcatcgca cgcgcgcagc gcgtgatcgg ccgtatcgaa
     4081 catgagcagc aacgcatcgc cgccccactt gaccagccag ccgccatggt cgtaggccgc
     4141 ggtcagcagc tcgctcagga ccacgtcgag gtggtcggac atttcctctg cgccgaagcg
     4201 gcccctggct gcgagccgct cggtgagccg ggtaaagccg gagatgtcag cgaaaaccag
     4261 ggtgccaacc aattgccgcg cgctgacctc gggtgtctcg ttcagccacg tacgcgcgag
     4321 ttcggagacg aacggctcga gatggaccga gtggttcggc gaacggtcag cgttgctggt
     4381 ggacaagacg ggtgtcctca ccgacggcgg atcttcatgg tcgcatgttt tgcgcgtgcg
     4441 ccaggtacgt ttccggcgtc cataggtacc gctcagttct gggccgacgc cgcggcatcc
     4501 agccggctca cagccaagcg cgccgccaca ctgtcgagca ggctaggcga tggcggcagc
     4561 tgagcgaacg cctcaaggaa tgcctcgccg ccgattcggt acaggacgct cggtacgacg
     4621 gttcgtacgg tcgccgtgcg tggcactccc tcaaggaggc cgatctcgcc gaaccagtcg
     4681 ccagcgtgga ggtcgttcac gtgacgatcg ccgacaagca cctcgagctc gcctgcccgc
     4741 accacgtaga acgcgtcggc aacatcatgt tcggcaatga tcgtcgtgcc tgctgaaatg
     4801 ccgagttcgg atatatcggc ggcaagccgc tcgagtacgg gccgcgacga cgcttgcagg
     4861 agcccgaggt gttggagcac gacgatccgc ggttcgagct cctcaaaccg gctcgtcgac
     4921 gcagtatcgg atcggcgcag caccggatag acgccgatca cgacgatcgg cgggagcacg
     4981 cccaatatgg tcaacgtccc cgttgtccca aatgcatgca ggctcagcgg ggtagccagc
     5041 gcaccgatcg cgaccgcccc gaagacgacg gacgtcaacc gtgctgtcag caacggcaat
     5101 ccttgcggca ggacgaaccg ttggagctcg gtgagcgcga ccacatcgac agccagcgag
     5161 ccggcgcctc gtagcgcctc gaatgcgacg accgctacgg aactatgact gtgcgcgatc
     5221 agcaccgacg gcacgcagta gacaaccatg cacagcacca gccagcctgc gagccggtgc
     5281 tcacgcacaa tccgattgac gacgggcgcg gccagcagcc cgccagtgcc caggccggcg
     5341 aacaactcgc cgtagccggc gtcaccgagg tgcagtcgcg tggtcgcgag aacagcgagt
     5401 agcaccgtgt cggtgccata gacaaggccc gacaggccca tcaaggcaac gaaaattcca
     5461 atcccggacg tcgtgtgtgc gcgcattttg cgggtcctgc ccgcagcttt cggatcaggg
     5521 gtcagtaaac gcagttgccc tggtgacgga agcccaaggc tgacgaccgc tgcaatagcg
     5581 acaagtacgg cgagtgcgag catttcaact gcgggactga tcacgagcag catcaggcca
     5641 ccgaccgccg gcccggcgag cagcaaaagc ttgtcggtgg ccgcttgcct cgacgcgacc
     5701 cgagagagat ccccgccgcc cgccacgatc ggcaggagca acgccgccgc aggggcctgc
     5761 ccactcgccg cgatccgcac aaccgcggcg agagcgatcg ccaccgcggg cgacgcgttc
     5821 accaagatgg cggtggccat cgcggccatg gcaattgcgc aacagatgtc agacaccacg
     5881 agcagccggc gaggcggaaa gcggtcgagt aacggcgcgg cgaagacgct cgtcacgatg
     5941 cccggcaggt agcgcgcggc cgcggcggcc gcaacccagg ccgacgagtg ggtcgcgttg
     6001 tagacgtaga cgacgagagc gacgctgtag gcccggttgg caatcgccgc cagcgcgagc
     6061 gcaacatcga gccggagacg tcgaaggcta gtcctcaggc gagcctccgc tcgaatccgt
     6121 gcccccgacc acctcgggac ggtaacccgt ctacgacggg tcgcggtgaa cggatggtga
     6181 ctgctgggtg aacgaccggt ccgaggagac cgtcagggag cgccgtccat tactgtttcc
     6241 tgctaacgac gagcccctcg cggcctgctc acggaggtgc agatgtcagc cgtcaatccg
     6301 agcgaactct cgcgcatagc gctgttcgcc ggcctggacc aacaggacca acgcgtcgtc
     6361 gcttcatggc tggacgtcgg ggagttcgac cccggctaca agctgacgca cgagggtgcc
     6421 tctggttatg cgttcttcgt gctgcacgcc ggagccgcga acgtcatcgt cggcggcaac
     6481 gtggtgcgca cattaggccc gggcgacttc ttcggcgaac tctcgatgct tggcgacggt
     6541 cggcaaaccg cgacagtgac catcacgact ccctcgacgg tctggacgat gttcggcacc
     6601 cgcttccgcg agctgcaaca gcagcacccg gcgatcgccg ctgctatcga aaaggtcgcc
     6661 gcagagcggg tgagcccgca gtagtagcag agcgggtgtg agcccgcggt agccccccgc
     6721 tcgcggcgct ccggtagtct ccggccgtcg gatccaatgg gacgccgaga ggagcgcgcg
     6781 cgtggacgcc accgctgaca ccctccagaa ggcgctgcgg atcaacctcg acccgcgctg
     6841 gtacggcacg atcgccgaga tcggcgccgg ccaggaagtc gctcgctggt tcttccgggc
     6901 cggcggtgcc gccggcaccg tggcgaagtc gatctcggcg tacgacatgg ccgtgagcga
     6961 tgccgtgtac ggcaagtccg g
//
LOCUS       DATCBJ010000415         3491 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_496747, whole genome shotgun sequence.
ACCESSION   DATCBJ010000415 DATCBJ010000000
VERSION     DATCBJ010000415.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 3491)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3491)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3491
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_496747"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1745
                     /locus_tag="VII50_08355"
     CDS             <1..1745
                     /locus_tag="VII50_08355"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEY5050899.1"
                     /translation="LLALYHWRPPASMWFLAGGLALFSVADCIYLVLVAHNSYQPGGL
                     DDTTWVVATAVVALAPGWSKKAGGAALPAWVLLGIPVVATLCTVALLVYDHGHMLHPI
                     AVALAAATIVAAIGRLVVSFREVTTLAHSRQLALTDELTGLGNRRAFYEHVDTYLAAD
                     PDRSGALFLLDLDRFKEVNDSLGHHAGDDLLLQVATRLTQGLTHEDGLLARLGGDEFS
                     IFVLDVDRDGAEMFAEMVRQTLLAPFTIDGITVRVAASVGISLFPKHGLEVSTLLRRA
                     DIAMYHAKDLRTGFRVYSESDDSLGGQDRLRTLEELRQVIQARKLAMHYQPKVDSQTS
                     YVSGVEALVRWDHPVHGLLPPDAFLPLAEDAGLMRDLTMAVLEQSLDQVVLWRAGGRM
                     LSVAVNLSASSLVDLELPERVHQLLVNRGLPASALELEITEDFLMGDRERAREILTQL
                     RTLGIRVAVDDFGTGYSSLAYLRELPIDELKLDRSFVQPMADDPRAAAIVRSTISLAH
                     SLGMRLVAEGVENEITAGHLALSGCDVSQGFFFSKALPPLELEQWLDDRPDMAGMEVN
                     AAHAGSRAEDGLAS"
     gene            1742..2803
                     /locus_tag="VII50_08360"
     CDS             1742..2803
                     /locus_tag="VII50_08360"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY5050900.1"
                     /translation="MTAAPLMAASTDLLSGTTGVQIVAGIDSVRAHRGELEALAQRCG
                     AAITARSTWTLANAGGGSQPWAVLVRDPSGFLRAATVLVEVRGAGRTSHIVTFAGSDL
                     GHRGAVLADDKLWARRLGIALSRTLPARTSGWAVELGPLDADSTTLAGFLAGFPQLVP
                     VEGDPIPMIRREAGLDEATDYLSPAMRRTLRKATNRLATDYRTLDVRFTRSQSEIRAL
                     LPGLEESHRNRDHDRGRSSDLDDDDTRQTWRSRLTDLALDGMLEVATAHIDGSLAAHV
                     IGIRDGETYRVLEGHFVTTWARYAPGRLVEAAVVQRMLDEPEMIALDWMTSVAPESLL
                     ATNAAAPMVVLRAGLAQRR"
     gene            complement(2800..>3491)
                     /locus_tag="VII50_08365"
     CDS             complement(2800..>3491)
                     /locus_tag="VII50_08365"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HEY5050901.1"
                     /translation="GYWPAGDVVTLHANLKGVDAGKGVWGTEDRTLNFSIGDSQISTV
                     NAVTHEMSVVVNGAVARTFAVSTGRDKYPTTSGIHVVLDREPSVIMNSATVGIPKGSP
                     DYYYETVLWDVRISWSGEYVHSAPWSVSAQGRANVSHGCVNVGPADAQWFYNLSRRGD
                     IVVVTGTPRPLESGNGWTDWNMSWAQWVSGSALTGSVSGAAASSSSPTPSSSYGSTAT
                     PPASATASSST"
BASE COUNT          538 a         1249 c         1118 g          586 t
ORIGIN      
        1 cgctcctcgc cctctaccac tggcgtccac cagcgagcat gtggttcttg gccgggggtc
       61 tcgccctctt ctctgtcgcc gactgcattt acctcgtcct ggtcgcacac aacagctacc
      121 agccgggcgg tctcgacgac accacatggg tcgttgctac cgccgttgtg gcactcgcgc
      181 ctggctggtc gaagaaagcc ggcggcgcgg cactgcccgc gtgggtgcta ctcggcattc
      241 ccgtcgtcgc caccttgtgc acggtcgcac tactggtcta cgaccacgga cacatgctgc
      301 accccatcgc cgtcgcactc gctgcggcga ccatcgtggc cgcgatcggc cgcctcgtcg
      361 tcagcttccg cgaagtcaca accctcgcgc acagcaggca gctcgccctc accgatgagc
      421 tgaccggcct cggcaaccgg cgcgcgttct acgaacacgt cgacacctac ctggcggcgg
      481 accccgaccg cagtggcgcg ctgttccttc tcgacctcga ccggttcaag gaagtcaacg
      541 actccctcgg ccaccacgcc ggggacgatc tcctcttgca ggtcgcgacc cgacttacgc
      601 agggcctgac tcacgaagac gggttgctcg cgcgcctcgg cggcgacgag ttctcgattt
      661 tcgttctgga cgtcgaccgg gacggcgccg agatgttcgc cgagatggtc cgccagacgc
      721 tgctcgcacc gttcactatc gacggaatca ccgtgcgggt ggccgccagc gtcggaatct
      781 cgctgttccc caagcacggc ctcgaagtct cgactctgct gcgccgcgcc gacattgcga
      841 tgtaccacgc caaggacctg cgcaccgggt tccgcgtcta cagcgaatcc gacgactcgc
      901 tcggaggcca ggaccggctg cgcacgctgg aagaactgcg ccaggtgatt caagcccgca
      961 agctcgcgat gcactaccag cccaaggtcg actcccagac ctcctacgtg tcgggcgttg
     1021 aagcccttgt gcgctgggac caccccgtac acgggttgct cccacccgac gcgttcttgc
     1081 cgctcgcgga ggatgcgggc ctcatgcgcg acctcacgat ggccgtgctc gagcagtcgc
     1141 tcgaccaggt cgtcttgtgg cgggccggcg gccggatgct cagtgtcgcc gtcaacctct
     1201 cggcgtcctc gctcgtcgac ctcgaactgc ccgagcgggt gcaccagctg ctggtgaacc
     1261 gcgggctgcc ggcgtccgct ctggaactcg aaatcactga ggacttcctg atgggcgacc
     1321 gcgagcgggc ccgcgaaatc ctcacccagc tccgcacgct cggcatccgc gttgcagtcg
     1381 acgacttcgg taccggttac tcctcgctcg cctacctgcg agagctgccg atcgacgagc
     1441 tgaagctgga ccgttcgttc gtccagccga tggccgacga cccgcgggcg gccgccatcg
     1501 tgcggtcgac gatcagcctg gcccactcgc tgggcatgcg gctggtcgca gagggtgtcg
     1561 agaacgagat cactgccggg cacctcgcgc tgtccggatg tgatgtctcg cagggattct
     1621 tcttctccaa ggccctgccg ccgctcgagc tcgaacagtg gctggacgac cggcccgaca
     1681 tggcgggcat ggaagtcaac gccgcccacg ccggttcccg cgccgaggac ggactggcct
     1741 cgtgaccgct gcgccactca tggcagccag cacggatctg ctttccggaa cgacgggggt
     1801 tcagatcgtc gcggggatcg acagcgtgcg cgcacatcgc ggcgagctcg aagcattggc
     1861 tcaacgctgc ggcgctgcga tcaccgcgcg aagcacctgg acgctcgcca acgcgggcgg
     1921 tgggtcgcag ccttgggcag tcctggtccg cgacccgtcc ggcttcctgc gcgccgccac
     1981 ggttctcgtg gaggtccgcg gtgcggggcg cacgtcgcac atcgtgacct tcgccgggag
     2041 cgatctcggc catcgcggcg ccgtgcttgc cgacgacaag ctgtgggcgc gtcgactggg
     2101 catcgcactg tcccggacgc tgcccgcgcg cacgagtggg tgggcggtcg aacttggccc
     2161 gctcgacgct gactcgacca cactcgccgg cttcctggcc ggcttccccc agcttgtccc
     2221 cgtcgagggc gacccgatcc cgatgatccg gcgagaggcc ggcctcgacg aggccaccga
     2281 ctacctgagc ccggcgatgc gccgcacgct gcgcaaggcg acgaaccgcc tggcaaccga
     2341 ctaccgcacc ctcgacgtcc gcttcactcg cagtcaaagc gagatccggg ccctgctgcc
     2401 gggcttggag gagtcacacc gaaaccggga ccacgaccgt gggcggtcca gtgacctcga
     2461 cgacgatgac acgcgccaga cctggcgcag ccggctgacg gatctcgcgc tcgacggcat
     2521 gctcgaggtt gcgacggcgc acatcgacgg ctcgcttgca gcccatgtga tcggtatccg
     2581 ggacggcgag acctaccgcg ttctcgaggg ccacttcgtt actacgtggg cccgttacgc
     2641 gcccggccgt ttggtcgaag cggcggtggt gcaacgcatg ttggacgagc ccgagatgat
     2701 cgcgctggac tggatgacgt cggtggcgcc ggaatcactg ctggcaacca atgccgccgc
     2761 accgatggtc gtgctgcgcg ccggtctggc ccagcgccgc taggtcgagg acgacgctgt
     2821 agcgctggcc ggcggggtcg cggtcgaccc gtaggacgac gacggcgtgg gcgaactcga
     2881 cgacgctgcc gcgccgctca ccgagccggt aagggcgcta ccggacaccc attgcgccca
     2941 gctcatgttc cagtcggtcc agccgttgcc cgactcgagt ggccgagggg tgcctgtcac
     3001 caccacgatg tcgccgcggc gcgacaggtt gtagaaccac tgggcgtccg ccggacccac
     3061 gttgacgcag ccatggctga cgttcgctct gccttgcgcg ctcaccgacc acggcgccga
     3121 gtgcacgtac tcaccggacc atgagatacg aacgtcccac aagaccgtct cgtagtagta
     3181 gtcggggctg cccttcggaa tcccgacagt cgcggagttc atgatgacgc tcggctccct
     3241 gtcgagcacg acgtggatgc ccgacgtcgt cggatacttg tcacgccccg tgctgaccgc
     3301 gaacgtcctg gccaccgcgc cgttgacgac gacgctcatc tcgtgcgtga ctgcattgac
     3361 ggtcgagatc tgcgagtcgc cgatgctgaa gttgagggtg cgatcctcgg tgccccacac
     3421 gcctttgccg gcgtcgacgc ctttcaggtt ggcgtgcagt gtcaccacgt caccggccgg
     3481 ccagtacccg a
//
LOCUS       DATCBJ010000416         3207 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_336761, whole genome shotgun sequence.
ACCESSION   DATCBJ010000416 DATCBJ010000000
VERSION     DATCBJ010000416.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 3207)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3207)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3207
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_336761"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..710
                     /gene="panC"
                     /locus_tag="VII50_08370"
     CDS             <1..710
                     /gene="panC"
                     /locus_tag="VII50_08370"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HEY5050902.1"
                     /translation="VNPLQFAPGEDLDRYPRTLEADLAMCAEVGVALAFVPSRIEMYP
                     REPVVSVIAGPMGQRFEGASRAGHFDGVLTVVAKLFGLVRPDVAVFGRKDAQQLALVR
                     AMVCDLDLPVRIEGVALIRDADGLALSSRNAYLTAEQRIAALALPRALQAGVTAASRG
                     QSPPDIVAAARAMLDGQRGVDTDYVALVDPDTFEDVDAGGRAVGAYRQAVLAVAARVG
                     ATRLIENESVEISGVTR"
     gene            707..2350
                     /locus_tag="VII50_08375"
     CDS             707..2350
                     /locus_tag="VII50_08375"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008734 - L-aspartate oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HEY5050903.1"
                     /translation="MSLIRRLAAAQPGWVARADIVVVGSGIAGLTTALKARRAGTVLL
                     VTKTELDAGSTRWAQGGIAAALGPGDSPADHLTDTLVAGAGLCDEDAVRVLVTEGPDL
                     VRELVELGTRFDVDHAGDLALTREGGHLRRRIAHRGDATGAEIERALIAAVKATEGIE
                     IIEHALVIDLLKTLDGRACGVTLHVIGAGRRDGVGAALGRAVVLATGGLGQVFASTTN
                     PPVATGDGGALALRAGADVTDMEFVQFHPTVLWLGPGAAGQQPLISEALRGEGAVLID
                     VLGRRFMVGQHPMAELAPRYVVAQAIVRQLRETGADHVLLDARAVPDIAKRFPTITAS
                     CRANGIDPVTTPIPVVPGAHHASGGVRTDLFGRTSVPGLYACGEVACTGVHGANRLAS
                     NSLLEGLVFAARIADELARSLPPAGEPAIDARDAGLLPSSALIGVQAAMTAGAGVLRS
                     ADSLAAAAASLARIGEQGRAGEVVEPCVEAWEATNLHTVASALVAGARLRAETRGCHW
                     REDYPETSPAWRGHLVTSLADDGAPQTVFEAATVAALMP"
     gene            2347..>3207
                     /gene="nadC"
                     /locus_tag="VII50_08380"
     CDS             2347..>3207
                     /gene="nadC"
                     /locus_tag="VII50_08380"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HEY5050904.1"
                     /translation="MTLPAVLDRRLADAGLDAVAVESLVSRALAEDLDGGVDVTSVAV
                     ISSDDEAAADFVCRAAGVVAGVPIASTVLWIATGGAVQVDERVVDGMRVEPGTIVLSA
                     AGATRSLLTAERTALNLLGHLSGIATLTRAWVDAIAGSGAAIRDTRKTTPGLRALEKY
                     AVRCGGGLNHRMSLSDAALIKDNHVLAAGGVAAAFRAVRTAFPQLPVEVECDTVRQVD
                     EALEAGADLILLDNMSLDSMRAAVALVHERGSSCRLEASGGLTLAGAAAVAATGVDYL
                     AVGALTHSAPV"
BASE COUNT          440 a         1037 c         1181 g          549 t
ORIGIN      
        1 tcgtgaatcc cctgcagttc gcgcccggcg aggacctcga tcggtatccg cggacccttg
       61 aggcggatct cgcgatgtgt gccgaagtag gcgtcgcgtt ggcgtttgtg ccgagtcgca
      121 tcgagatgta tccacgcgag ccggtggtgt cggtgattgc cggtccgatg ggtcaacgat
      181 tcgagggagc gagccgcgcc gggcacttcg acggcgtact cacggtggtc gcgaaactgt
      241 tcggcctcgt gcgcccggac gtcgcggtgt tcggtcgcaa ggacgcccag cagcttgcgc
      301 tcgtgcgggc gatggtgtgc gatctcgacc tgccggtccg aatcgaaggc gtcgcactca
      361 tccgggacgc cgacgggttg gcgttgtcga gccgcaacgc gtacctcacc gcggagcagc
      421 gcatcgccgc gcttgcgttg ccgcgcgcgc tgcaggctgg cgtcacggcc gcgagcaggg
      481 gtcagtcgcc gccagacatc gttgctgccg cccgggcgat gcttgacggg cagcgcggtg
      541 ttgacaccga ctacgtcgcc ctcgttgacc ccgatacgtt cgaggacgtc gacgccgggg
      601 gtcgcgctgt gggcgcctac cgccaggcgg tcctcgcggt agccgcccgc gtcggagcga
      661 cccggctgat cgagaatgag tccgtggaga tcagcggagt gacgaggtga gcttgatccg
      721 acggctggcc gccgcacagc ctggctgggt tgcgcgtgcc gacatcgtgg tggtcggcag
      781 cggcatcgcg ggcctgacca cagcgttgaa ggcgcgacgg gccggcactg tgctgctggt
      841 gacgaagacc gaactcgatg ccggctcgac tcgctgggca cagggcggca tcgcggccgc
      901 gctcgggcct ggggacagtc ccgccgatca cctaaccgac acgctcgtcg ccggtgcagg
      961 tctgtgcgac gaagacgccg tacgggtgct ggtcaccgaa ggacccgacc tcgtgcgcga
     1021 actcgtcgag cttggcaccc ggttcgacgt cgaccatgcc ggcgatctcg cgttgacgcg
     1081 cgaaggcggc catctacgac gccggattgc gcaccgcggt gacgcgaccg gggcggagat
     1141 cgagcgcgcg ctgatcgccg ccgtgaaggc gaccgaaggc atcgaaatca tcgagcacgc
     1201 cctcgtcatc gacctgctga agaccctcga cggccgggcc tgcggtgtca ccctgcacgt
     1261 gattggagcc gggcgtcgtg acggcgtcgg cgcagctctc ggacgcgcgg tcgtgctggc
     1321 caccggtggt ctcgggcaag tgttcgcatc gacgaccaac ccgccggtgg cgaccggcga
     1381 cggcggagca ctcgcgttgc gcgcgggcgc cgacgtgacc gacatggagt tcgtccaatt
     1441 ccacccgacc gtgctgtggc tcggaccggg tgcggccggc cagcagccgc tcatctccga
     1501 ggcactgcgc ggggagggcg cggtgctcat cgacgtactc ggacgccgct tcatggtcgg
     1561 ccagcacccg atggccgagc tcgcgccgcg ctatgtcgtg gcgcaggcga ttgtgcgcca
     1621 gttgcgcgag acgggcgccg accatgtgct tctcgatgca cgtgcggtgc ccgatatagc
     1681 gaagcgtttc cccaccatca ccgcgtcctg ccgcgcgaac ggcatcgacc cggtgacgac
     1741 acctatcccg gtggtgcccg gcgcgcatca tgcgagcggt ggcgtgcgga ccgatctgtt
     1801 cggccgcacc agcgttcctg gcttgtacgc atgcggcgag gtggcgtgca cgggcgtgca
     1861 tggcgcgaac cggctggcct cgaacagcct ccttgagggg ttggtgttcg cggctcggat
     1921 tgcggatgag ttggcgcgct cgctgccgcc cgcgggagaa cctgcgatcg atgcgcggga
     1981 cgccggactg ttgccgtcat cggcactgat cggcgtccag gctgccatga ctgccggtgc
     2041 cggcgtgctg cgtagcgctg acagccttgc ggctgccgca gcgtcgctgg cccgtattgg
     2101 tgagcagggt cgtgccggtg aggttgtcga gccgtgcgtc gaggcatggg aggcgaccaa
     2161 cttgcatacc gtcgcgagcg cgctcgttgc gggcgcgcgg ctgcgtgcgg agacccgcgg
     2221 gtgtcactgg cgtgaggact accccgagac gtcccccgcg tggcgcgggc atctcgtgac
     2281 gtcgcttgcc gatgacggcg caccgcaaac tgtctttgag gccgcaaccg ttgccgcgct
     2341 catgccatga ccctgcctgc cgtgctcgat cggcgtctcg ctgatgccgg actcgacgca
     2401 gttgccgttg agagcctggt tagcagggcg cttgcggagg atttggacgg cggcgtcgac
     2461 gtgaccagcg tggcggtcat ctcgtcggac gacgaggcag ccgctgattt cgtctgtcgt
     2521 gcggctgggg tcgttgcagg tgtgccgatc gcctcgacgg tgctgtggat cgcgaccggc
     2581 ggcgctgtcc aggtcgacga aagggtcgtc gacggcatgc gggtggagcc gggcaccatc
     2641 gtgttgtcgg cagctggcgc gacgcgttcg ctgctcactg ccgaacgcac cgcgctgaac
     2701 ctgctggggc acctctccgg catcgcgacc ctcacgcgcg cttgggtcga tgcgatcgcg
     2761 ggcagcggcg ccgcgatccg cgacaccagg aagacgacgc cgggtctgcg ggcgctggag
     2821 aagtacgcgg ttcggtgcgg gggcggcttg aaccaccgga tgtcgctgtc cgatgcggcc
     2881 ttgatcaagg acaatcacgt tctcgctgcg ggtggggttg cggccgcgtt tcgtgccgtg
     2941 cgcaccgcct ttccgcagct accggtcgaa gtcgagtgcg acaccgtccg gcaggtggat
     3001 gaggcgttgg aggcgggtgc cgacctgatc ttgctcgaca acatgtcgct ggactccatg
     3061 cgggcggccg tggcgttggt gcacgaacgc ggcagctcgt gccgccttga agccagcggt
     3121 ggcctgacct tggccggagc cgcagcggtc gccgcgacgg gtgtcgatta cctcgcggtc
     3181 ggtgctttga cacactctgc gccagtg
//
LOCUS       DATCBJ010000417         3015 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_517066, whole genome shotgun sequence.
ACCESSION   DATCBJ010000417 DATCBJ010000000
VERSION     DATCBJ010000417.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 3015)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3015)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3015
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_517066"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..298
                     /locus_tag="VII50_08385"
     CDS             <1..298
                     /locus_tag="VII50_08385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009074248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY5050905.1"
                     /translation="LTLAMAADHMHEGIRVNCVNPGTADTPWVGRLLDAADDPVAERA
                     ALEARQPLGRLVSADEIAEAIIYLASPAAASTTGTALAVDGGMYGLRLPGRPAT"
     gene            complement(306..1148)
                     /locus_tag="VII50_08390"
     CDS             complement(306..1148)
                     /locus_tag="VII50_08390"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEY5050906.1"
                     /translation="MTPARVIDSHIHLWDPAVLRYPWLESVEGLARPFLPADLHRSGG
                     PPEWIMVQADAQDELAELDWIASLASSEPALVGMVVRAPVEIGRGVLDLVSRLAVTPP
                     TVGIRRLIQDEGAGFSLTASFIDGVRAVGEVGLPFDICVRAAERADVVQLVDACPDVS
                     FVLDHLGKPGVADGEWEPWRTQLAELAQRPNLTCKLSGSTTEAGPRWREDSVRPYLTH
                     ALESFGPQRCMFGSDWPVATVTTDYQRWFDLVSDVVSGCSQAEIDAVFAGTAAQVYAV
                     PARP"
     gene            complement(1185..>3015)
                     /locus_tag="VII50_08395"
     CDS             complement(1185..>3015)
                     /locus_tag="VII50_08395"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional diguanylate
                     cyclase/phosphodiesterase"
                     /protein_id="HEY5050907.1"
                     /translation="SLLADDIVGPTLGTKPLEGSIPPNAATAIAAVFSRASGEHRLLR
                     LRVRDMSGVVVWSDDGSGHSTADANDAEALDATHGEVVSALSRLNSDANDHGPVGARV
                     VEIYQPIDVGTARTQVGVLEMYLPYAPIQQDVTAGLHRMYELLAAGLLALWLVLGAIS
                     ASTTRRLRKRAERLQYIAHHHVPSGMLNGEGFADALHVRRGAAAADDPAATPSLAVID
                     VARFREINEALGRARGDEVLLELGRRLADAAGTDAVVARLGGAGFALASFTVRDSDLR
                     AWALTLQAAVAEPVLADGVPVLVEVAIGYYRAAAGEPVDELLVRVDAALGRAKLSVDK
                     CAGYVDRPDRAASNVALLPRFRRAISAGELELYYQPKTLLSDGSVVAMEALIRWNLDG
                     EVLPPGVFLPAIEQTAMIHPLTDWVARTALAQLNAWGPAMAGLEVAINVSARNLSDPG
                     FAARLLAIVAESGVAPQRLIVEITETALFIDPDAARVCLGTLHDAGIALSIDDFGQGQ
                     TSLAYLAALPVDELKIDRAFVSRVVADPTHAAIVRSIIQLGHTLGLRVVAEGIERESE
                     YAQLVALGADIAQGYVIARPMSAAAFVDWLAARVTTGSHAAAS"
BASE COUNT          538 a         1050 c          988 g          439 t
ORIGIN      
        1 gctgactctc gcgatggcag ccgaccatat gcacgaaggg atccgggtca actgcgtcaa
       61 tcccggcacg gccgacactc cgtgggtcgg acggctgctc gatgcagccg acgacccggt
      121 agccgaacgg gccgccctcg aagcacggca accgcttggt cgtttggtga gtgccgacga
      181 aatcgccgag gcgatcatct acctggcatc acccgccgcg gcatctacga ccggcaccgc
      241 gctggccgtc gacggcggca tgtacggcct gcgcttgccg ggccgccccg caacctgaac
      301 cagggtcagg gccgggcggg cacggcgtac acctgcgcgg ccgtccctgc gaacaccgca
      361 tcgatctctg cttgcgagca accgctgacc acgtcgctca ccaggtcgaa ccaccgctga
      421 tagtcggtcg tcaccgtcgc caccggccag tcgctgccga acatgcatcg ctgcggtccg
      481 aaagactcca gcgcatgcgt gagatagggc cgcacgctgt cctcgcgcca gcgtggaccg
      541 gcttcggtcg tcgacccgga gagcttgcaa gtgagattcg gccgttgcgc gagttcggcc
      601 aactgagtac gccacggctc ccactcgccg tccgcgaccc cgggcttgcc cagatgatcg
      661 aggacgaagc tcacatcagg acacgcgtcg acaagctgca cgacgtccgc ccgctctgcg
      721 gcccgcacgc agatgtcgaa cggcagcccg acctcaccta ccgcacgcac gccgtcgata
      781 aagctggccg tcaacgaaaa cccggcaccc tcgtcttgga tcagccgccg gatgccgacg
      841 gtcggcggcg tgactgccaa ccgggagacg aggtcgagga cacctcgccc gatctcgacc
      901 ggcgcgcgta cgaccatgcc aacaagggct ggctcggacg acgcgaggct ggcgatccag
      961 tcgagttctg ccagttcatc ctgcgcatct gcctgcacca tgatccattc cggcgggcca
     1021 ccgcttctgt gcaggtcggc gggcaggaac ggacgcgcca gcccctcgac ggattcgagc
     1081 cacggatacc gcagcaccgc gggatcccaa agatggatgt gcgagtcgat cacccgcgcc
     1141 ggtgtcattg ctcgatacct ccgacgaccg ggactgaatg agacttagga cgccgccgcg
     1201 tgcgatcccg tcgtcacccg cgcggctagc cagtctacga atgcagcggc ggacatcggc
     1261 cgcgcgatca cgtagccctg cgcaatgtcg gcgccaagcg ccacgagctg ggcgtactcg
     1321 gactcccgct cgatgccttc ggcaacgacg cgaagaccca acgtgtgtcc gagctggatg
     1381 atcgaacgca cgatcgcggc atgcgtcgga tcggcgacga ccctggagac gaacgcgcgg
     1441 tcgatcttga gctcgtccac gggcagcgcg gcgaggtagg cgagggaggt ctgcccctga
     1501 ccgaagtcgt cgatggacag agcaatcccg gcgtcatgca gagtgccgag gcacacccgc
     1561 gcggcgtccg gatcgatgaa cagcgcagtc tcggtgatct cgacgatcag tcgctgcggc
     1621 gccacgccgg actccgccac gatcgcgagc aaacgcgccg cgaaaccggg gtcggacagg
     1681 ttgcgcgccg agacgttgat ggcaacctcg agaccggcca tggccgggcc ccacgcgttc
     1741 agttgggcga gagccgttcg cgccacccag tcggtgagcg ggtggatcat cgccgtctgc
     1801 tcgatagccg gcaggaagac ccccggcggc aacacctcgc cgtccaggtt ccatcggatc
     1861 agtgcctcca tcgccacgac actgccgtcg gagagcagcg tcttaggctg gtagtacagc
     1921 tcgagctccc cggccgagat agcacggcgg aatcgcggca gtagcgcaac gttggacgcg
     1981 gcgcggtccg ggcggtcaac gtatcccgcg cacttgtcga ccgacagctt cgcgcgcccg
     2041 agggctgcat cgacgcgaac aagcagctcg tcgacgggtt cgcccgcagc ggcccggtag
     2101 taaccgatgg ccacctcgac gagcaccgga acgccatccg caagaaccgg ctcagcgacc
     2161 gccgcttgca gcgtcaatgc ccaggcgcgc agatcgctgt ctcgcacggt gaagctggcg
     2221 agcgcgaacc cggcaccacc caggcgcgcg acgacggcat cggtgcccgc tgcgtcagca
     2281 agtcggcgtc cgagctcgag caggacttcg tctccgcggg cccggcccaa cgcctcgttg
     2341 atctcccgga aacgagcgac gtcgatcacc gctagcgacg gtgtcgcggc ggggtcgtcg
     2401 gccgcggcgg cgccgcggcg tacgtgcaac gcgtcggcga agccttcacc gttcagcatc
     2461 ccggatggca cgtggtgatg ggcgatgtac tgaagtcgtt cggcgcgctt gcgtaaccgt
     2521 cgcgtggtcg aggcggagat cgcacccaag acaagccaca gtgcaagcag tccggcggcc
     2581 agcagctcgt acatccgatg gaggcctgcg gtcacgtcct gctggatcgg ggcgtacggc
     2641 aggtacatct cgagcacgcc gacctgagtg cgggcggtgc cgacatcgat cggctgataa
     2701 atctcgacca cccgcgcgcc aacgggccca tggtcgttgg catccgaatt caatctgctg
     2761 agggcgctca cgacctcgcc gtgggtcgca tcgagcgcct cggcgtcgtt cgcatctgcc
     2821 gtcgagtggc cactgccgtc gtcggaccac accaccacac cggacatgtc gcgcacccgg
     2881 agccggagca atcggtgttc accggaggct cggctgaaca cggccgcgat cgcggtcgct
     2941 gcgttaggcg ggatcgatcc ctcgaggggc ttggtaccaa gcgtcggtcc gacgatgtca
     3001 tcggcgagca gcgaa
//
LOCUS       DATCBJ010000418         2211 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_191173, whole genome shotgun sequence.
ACCESSION   DATCBJ010000418 DATCBJ010000000
VERSION     DATCBJ010000418.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2211)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2211)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2211
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_191173"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            12..128
                     /locus_tag="VII50_08400"
     CDS             12..128
                     /locus_tag="VII50_08400"
                     /inference="COORDINATES: protein motif:HMM:NF024672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HEY5050908.1"
                     /translation="MLLAEGTVRVNGEPETRRGRQLVKGDVVTVGQQQLRVG"
     gene            207..1034
                     /gene="dapF"
                     /locus_tag="VII50_08405"
     CDS             207..1034
                     /gene="dapF"
                     /locus_tag="VII50_08405"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HEY5050909.1"
                     /translation="MDGIAFVKGHGTRNDFVLLPDADGTLDLTAELAIALCDRRGGIG
                     ADGVLRVVKATADPDATAMADQATWFMDYRNADGSLSETCGNGLRVFARYLVDAGLAD
                     SGEFAIATRGGIVIVWADVDGDVTVDMGPPAISSGATAVMGGQVFEGVAVSMGNPHLV
                     CVTDTDIASLDLTTMPDVDVLAFPEGVNVEFVNIVGERHAVMRVHERGSGETQSCGSG
                     ACAVVAALAAIDGTTDGEFIVDVPGGRLTVAFNDAGHLLLRGPAVLVAEGHWRTNQS"
     gene            complement(1059..>2211)
                     /locus_tag="VII50_08410"
     CDS             complement(1059..>2211)
                     /locus_tag="VII50_08410"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY5050910.1"
                     /translation="LALPLVAVKTLHATAFEVGVLAACETAAFLLVGLPAGAWCDRVR
                     RRPILIAGDIGRALVLGSVPIAALMHRLTIGQLFVVALLTGVLTVFFDVSYQSYLPEL
                     VSNDELVEGNAKLEATRGVAAVTGPSIGGFLVQAITAPYAVLVDAVSFLWSAGWVTAI
                     RAPEEKPVAPPQRHLGKEIAEGLRFVLGNPILRKIAGCTGTSNLFNSASYAVMIVFFV
                     RTVHLSAGGIGLLFSIGSVGGLVGALCSGYLMRKVGQARLIWVSALIFAPPDLLLPLT
                     HHDARLIFAGTALFAASVGATVYNVAQVSFRQALCPRPLLGRMNATMRFLVWGTQPIG
                     GILGGWLATTVGLRDALWIAAACSQVAPFWVFFSPLRGMRDLPMSSQTE"
BASE COUNT          398 a          731 c          757 g          325 t
ORIGIN      
        1 cggacgcgaa gatgctgctc gccgaaggca cggtccgcgt caacggcgag cccgagacgc
       61 gccgcggtcg gcaactggtg aagggcgacg tcgtcaccgt tgggcagcag cagttgcgcg
      121 tcggctagcg gtccgccgcg acgccagcac ctcgacgtcc ggttaaggac gacgcgacgg
      181 ctgcgacgag ccgtaccatc gccactgtgg acggcatcgc gttcgtcaag ggtcacggca
      241 cccgcaacga cttcgtgctg cttcccgacg ccgacggcac gctcgacctg accgccgaac
      301 tcgcgattgc cctgtgcgac cgccgaggcg gcatcggagc tgacggggtg ctccgggtcg
      361 tcaaagcgac ggccgatcca gacgcaacag cgatggccga ccaggcgacc tggttcatgg
      421 actaccgcaa cgccgacggc agtctgtcgg aaacgtgcgg caacgggctg cgggtgttcg
      481 cgcgctacct ggttgacgcg gggttggctg actcgggcga gttcgccatc gcgacccgcg
      541 gcggcatcgt catcgtgtgg gccgacgtcg acggcgatgt gacggtcgac atgggtccgc
      601 ccgcgatctc gtccggtgct accgccgtga tgggcggcca ggtcttcgaa ggcgtcgccg
      661 tgtcgatggg caatccgcac ctggtctgcg tgaccgacac cgacatcgca tcgctcgacc
      721 tgacaacgat gccggacgtc gatgtgctcg cgtttcccga aggtgtcaac gtcgagttcg
      781 tcaacatcgt gggcgaacgg cacgccgtca tgcgggtgca cgagcggggc tcgggcgaga
      841 cacagtcctg cggcagcggc gcatgcgcag tggtcgccgc gctggcggca atcgacggca
      901 cgaccgatgg tgagttcatc gtcgacgtgc cgggcggccg gcttactgtt gcgttcaacg
      961 acgccggcca tctgttactc cgcggccccg ccgtgctcgt cgccgaaggg cactggcgca
     1021 cgaaccagag ctgattcgct acgccggctg attcgctgct attcggtttg actcgacatg
     1081 ggtaggtcgc gcatgccgcg cagtggcgag aagaacaccc agaacggcgc aacctgcgag
     1141 caggccgccg cgatccacag cgcgtcgcgc agaccgacag tggtcgccag ccagccgccg
     1201 agtatgccgc cgatcggctg ggttccccag accaggaatc gcatcgtggc gttcatccgc
     1261 ccgagaagcg ggcgcggaca cagggcttgc cggaagctca cctgggcgac gttgtagacg
     1321 gttgccccga ccgaggcggc gaacagggcc gtgccggcaa atatgaggcg cgcgtcgtga
     1381 tgggtcagcg gcaacagcag gtcgggcggg gcgaagatca gcgcactcac ccagatcagc
     1441 cgagcctgac cgaccttgcg catcaagtag ccgctgcaca gggctccgac caagccgccg
     1501 accgagccga tcgagaacag caaaccgatt ccgccggccg acaggtgcac ggtgcggacg
     1561 aagaacacga tcatgaccgc gtagctggcg ctgttgaaca ggtttgacgt gccggtgcag
     1621 ccggcgatct tgcgcaggat cggattgccc aggacgaaac gcagaccctc ggcaatctcc
     1681 ttgcccagat gtctttgcgg tggcgcgacc ggcttttctt caggtgcccg gatagcagtg
     1741 acccaaccgg cagaccacag gaagctgacc gcgtcgacca gcaccgcgta aggagcggtg
     1801 atggcctgca ccaggaagcc accgatgctc ggaccggtca ccgcagctac gccgcgggtc
     1861 gcctcgagtt tcgcgttccc ctcgacgagt tcgtcgttgc tcacgagctc gggcaggtag
     1921 ctctggtagg agacgtcgaa gaaaaccgtc agcacgccgg tgagcaacgc gacgacgaac
     1981 agttggccga tggtgagacg gtgcatcagg gcagcgatcg gcacgctccc gagcaccagc
     2041 gcgcgcccga tatcgccggc gatgaggatc ggtcgccgcc ggacccggtc gcaccatgca
     2101 cccgccggca gtccgaccag taggaacgct gcggtctcac aggcggcgag cacgccgacc
     2161 tcgaacgccg tggcgtgcag cgtcttcacg gcgaccagcg gcagggccaa c
//
LOCUS       DATCBJ010000419         2362 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_507494, whole genome shotgun sequence.
ACCESSION   DATCBJ010000419 DATCBJ010000000
VERSION     DATCBJ010000419.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2362)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2362)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2362
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_507494"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..795)
                     /locus_tag="VII50_08415"
     CDS             complement(<1..795)
                     /locus_tag="VII50_08415"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HEY5050911.1"
                     /translation="MATVAEPIVHGHPVESELKTNTLTLFDTTVIATSSVAPAYTLAA
                     TAAFLIVAVGLASPAAILVGFLPVLCIAIAYYYLNKQDPNCGASYSWVSRTLNPYIGW
                     VSGWIQLAANVLFCAAAPLVAGAYSLQLLNSFGWISADAANDNRLIAGVGVLWLLLVT
                     FMVARGIRVTANFQWILVFIEYFIVLAFALWAIGKIAGGHAAANAVSAQWYNPLQLHD
                     LNGLATGSALAVFFFWGWDTAANVNEESKDAETSPGLAGIYSMFILL"
     gene            complement(839..2281)
                     /locus_tag="VII50_08420"
     CDS             complement(839..2281)
                     /locus_tag="VII50_08420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-aminobutyraldehyde dehydrogenase"
                     /protein_id="HEY5050912.1"
                     /translation="MSTVAVRTLRNFVDGEYRDASSDASTPLINPVTGEEFARAPVSS
                     PADVDAALQAARRAFPGWRDTTPRERSLAMLRIADAFEKRSEEFVALESENTGKPLSL
                     TRSEEIPQLIDPVRFFAGCARILEGKSAGEYMAGHTSMIRREPLGVCAQVTPWNYPML
                     MAIWKWSPAIAAGNTVVLKPSDTTPVTTLLMAEIMAEFLPPGVFNVICGDPSTGAALV
                     THKIPAMASITGSVRAGMEVARAAAGDLKRVHLELGGKAPVIVFDDSDVASTAEAVAM
                     AGYFNAGQDCTAATRVLASSAIHDELVAALAEQAAANKTGAPDDDEVAFGPLNNARQL
                     GRVQELVGNVPSHADVVAGGHRVGDRGYFHEATVIAGLRQDDDLIQREIFGPVITVQR
                     FDDESKALEWANGVEYGLSASVWTKDHGRAMRMARALDFGCVWINTHIPLISEMPHGG
                     YKHSGYGKDLSMYGFEDYTRIKHVMSNIEA"
BASE COUNT          412 a          758 c          777 g          415 t
ORIGIN      
        1 tagaaggatg aacatgctgt agatgcctgc caggccaggc gatgtctcag cgtccttcga
       61 ctcctcgttg acgttcgcgg cagtgtccca gccccagaag aagaagactg ccagcgctga
      121 tccggtcgcc agaccgttga ggtcgtgcaa ctgcagcggg ttgtaccact gcgcggagac
      181 cgcattggcc gccgcgtgcc cgccggcgat cttgccgatc gcccagagcg cgaatgccag
      241 gacgatgaag tactcgatga acacgaggat ccactggaag ttcgccgtca cccgaatgcc
      301 acgcgcgacc atgaaggtca cgagcagcaa ccagagcacg ccgaccccag cgatgaggcg
      361 gttgtcgttg gccgcatcgg cgctgatcca gccaaagctg ttcagcagtt gcagcgaata
      421 cgcgccggcg accagcgggg cggccgcgca gaagaggacg ttggcagcaa gttgaatcca
      481 cccggatacc cacccgatgt atgggttgag cgtccggctg acccaggagt aggacgcgcc
      541 gcagttgggg tcctgcttgt tcaggtagta gtaggcgatg gcgatgcaca gcaccggcag
      601 gaatcccacg aggatcgcgg ccggcgacgc caggccaacg gcgacgatga gaaacgccgc
      661 ggtggcggcg agcgtatacg cgggcgcgac actcgacgtc gcgatgacgg tcgtgtcgaa
      721 gagcgtcagc gtgttggtct tgagctcgga ttcgaccggg tgcccatgaa cgattggctc
      781 cgctaccgtc gccatgtttt cctcctcacc atgtcctcct gcgtcgagcc tggattgatc
      841 aggcttcgat gttgctcatc acgtgcttga tgcgggtgta gtcctcgaac ccgtacatcg
      901 agaggtcctt gccatacccc gaatgcttgt agcccccatg cggcatctcg gagatcagcg
      961 ggatgtgtgt gttgatccag acgcagccga agtccagcgc ccgcgccatg cgcatcgctc
     1021 ggccatggtc cttggtccag acactcgcgc tcaggccata ttcgacaccg ttggcccatt
     1081 ccagcgcctt cgactcgtcg tcgaagcgct gcacggtgat caccgggccg aagatctcgc
     1141 gctggatcag gtcgtcgtcc tggcggagcc cggcgatgac ggttgcctcg tggaagtagc
     1201 cgcgatcacc gacgcgatgc ccgccagcga cgacgtcagc gtgtgagggc acgttgccca
     1261 cgagctcctg cacccggccg agctgcctgg cattgttgag cggcccgaag gcaacctcgt
     1321 cgtcatcagg tgcgccggtc ttgttggcgg cggcctgctc ggcgagtgcg gcaaccagct
     1381 catcgtggat cgccgacgac gcgagcacgc gcgtggcggc ggtgcaatcc tggccggcgt
     1441 tgaaataccc ggccatggcg acagcctctg cggtggatgc cacgtcagag tcgtcgaaga
     1501 cgatcaccgg tgccttgccg ccgagctcga ggtgcacgcg cttgaggtca cccgcggctg
     1561 cgcgagccac ctccatcccg gcgcgcacac tgccggtgat cgacgccatt gccgggatct
     1621 tgtgcgtcac cagcgctgcg ccggtgctcg gatcgccaca gatgacgttg aagacacccg
     1681 gggggaggaa ctcggccatg atctcggcca tcaacaaggt ggtgactggt gtcgtgtcgg
     1741 acggcttgag gaccacggta ttgccggcgg cgatcgcggg cgaccacttc cagatcgcca
     1801 tcaacatcgg gtagttccac ggtgtgacct gggcgcagac gccgagcggt tcgcggcgga
     1861 tcatcgacgt gtggccggcc atgtactcgc cggcactctt accctcgagg atccgtgcgc
     1921 agccggcaaa gaaacgaacc gggtcgatca gctgaggaat ctcctcgctg cgcgtgagcg
     1981 acagcggctt gccggtgttc tcgctttcga gcgccacgaa ttcctcggag cgcttctcga
     2041 acgcatcggc gatgcgcagc atcgcgaggc tccgttctct cggcgtcgtg tcgcgccagc
     2101 cgggaaaggc gcgccgggcg gcctggagcg ctgcatcgac gtcggccggg ctcgacaccg
     2161 gtgcccgcgc gaactcctca cccgtgaccg ggttgatcag tggtgtcgag gcgtcgctgc
     2221 tcgcgtcgcg atattcaccg tcgacgaagt tgcgcagcgt gcgcaccgcg acggtactca
     2281 tgccttcaga tccctcgcga gctcgcggaa gttggcgacg tagcgcttca cgtcatccga
     2341 cgagtgctgc acggacagcg tc
//
LOCUS       DATCBJ010000420         7337 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_333746, whole genome shotgun sequence.
ACCESSION   DATCBJ010000420 DATCBJ010000000
VERSION     DATCBJ010000420.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 7337)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7337)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7337
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_333746"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            136..1248
                     /locus_tag="VII50_08425"
     CDS             136..1248
                     /locus_tag="VII50_08425"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HEY5050913.1"
                     /translation="MREYLITLLVAATVTYLSTPIARRFAVRVGAFTGVRDRDVHSIP
                     TPRLGGAAMFAGMAAALLVASRMPTLQVVNQRFSEPRALLMGGGIVFVFGALDDRFNL
                     DAVTKLAGQILAASVMVLQGVQLLWLPIPGQGTLILGQDWSVVLTVSFIVIVINAVNF
                     IDGLDGLAAGVVGIAALAFFALSYNLSVQSHLPYALPPMLIAIIVAGMCLGFLPHNFH
                     PARVFMGDSGSMLIGLLLAASTVSLTGQVDVVTQYQQYGAFLPLLLPFAILLVPFADL
                     ILAVARRTKQGRAPWSPDKQHLHHRLLELGHSQRRAVLIMYFWSALFGAGVVALSVTK
                     GPLVVVALGGALAAIALVLVNMPRLRAAQRSRHGPA"
     gene            1283..1507
                     /locus_tag="VII50_08430"
     CDS             1283..1507
                     /locus_tag="VII50_08430"
                     /inference="COORDINATES: protein motif:HMM:NF021073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AtpZ/AtpI family protein"
                     /protein_id="HEY5050914.1"
                     /translation="MTSAPQRPQLGLYALIGIGTLNLVSLLVGLGIGWFLDGRFHSFP
                     AATLIGLALGIAAGLTTSWLQIRRYFTDAK"
     gene            1542..1937
                     /locus_tag="VII50_08435"
     CDS             1542..1937
                     /locus_tag="VII50_08435"
                     /inference="COORDINATES: protein motif:HMM:NF015834.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase subunit I"
                     /protein_id="HEY5050915.1"
                     /translation="MNSTAPLMVPVGTPRRVFIIAAVLGGVGLLIASILGHVVVGLLL
                     LAGLVLGAVNNILTVRSILKFTAAGDPDRKKFAKASLLRLAYISLIAALFLIVFRREG
                     IAVLAGLAVFHLLAALATSVPALKEFRKA"
     gene            1934..2797
                     /gene="atpB"
                     /locus_tag="VII50_08440"
     CDS             1934..2797
                     /gene="atpB"
                     /locus_tag="VII50_08440"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HEY5050916.1"
                     /translation="MSWSSTAPAHVVAATIDVGVHPHVKILGLTINSDTVLTTLIAGA
                     IVVGLGLYMRVRAQSGVPSKLQLIFELIVDTVNKQVDESMGIEVAPFVVPLAVTLFLF
                     ILLCNWIGLIPSGHPEKLPAPTADINLTLAMAFAVIIPLHIVSVRRRGLKAYVKHFFQ
                     PYPIMFPINLIEEIVKPFTLALRLFGNLISGAIMVALLALLPAYILWAPQGIWKVIDA
                     LVGVIQAFIFALLTILYFAFATTTENHGATDSHSGTDGHPGTDGHPVTDGHPVTDGHL
                     VRDGQPVIEAH"
     gene            2828..3094
                     /locus_tag="VII50_08445"
     CDS             2828..3094
                     /locus_tag="VII50_08445"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HEY5050917.1"
                     /translation="MATDAANLDSAIKTAGAFVGGGLALAGGAIGAAVGDGLAGAQTI
                     AGVARQPESQPRLMPIFFLTVGLVEAMYFINLAFMAMFVFVLGK"
     gene            3107..3691
                     /locus_tag="VII50_08450"
     CDS             3107..3691
                     /locus_tag="VII50_08450"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HEY5050918.1"
                     /translation="MPIFIAAASPSPAATPAKTNNFLIPNGTFIAELIIFLIILAVIG
                     KYVVPFVNLKLAERQEAIRLEFKELEDARAKLEATEAEYRDLIASARADAARQREEAR
                     EEGAQILADLRAQAQVESERIKQAAHQQIEAERSRTVEALRAEVGSLAVDLAGRVVGE
                     SLTDDARQRRVVERFLIEVESRAGEAAPGQQVRG"
     gene            3691..4503
                     /locus_tag="VII50_08455"
     CDS             3691..4503
                     /locus_tag="VII50_08455"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF009967.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HEY5050919.1"
                     /translation="MQGASREALAQAWTSVERQLTPSSVDAAVIGDELFGVADVIDTQ
                     VALRRALSDPALEGDRKAGLIEAVLGSHVGAVTLAIVTEVVRARWSRMRDLTDAIETL
                     AVLAVLIAADRAGITDDVEDELFRFGRVVEARPDLRDALRSQALPAANKVALVGALLD
                     GKATVTTTRLVTQIALHPRGRVPEDALAEYGLVASRRRDRLVARVATAIALSDDERLR
                     LQAALAAMYGHDVHLEVELNEAVVGGVVVQVGDEVLDGSVAGRLAEARRRLE"
     gene            4564..6213
                     /gene="atpA"
                     /locus_tag="VII50_08460"
     CDS             4564..6213
                     /gene="atpA"
                     /locus_tag="VII50_08460"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HEY5050920.1"
                     /translation="MAELTIRPEEIRDALERFVSSYTPDVASREEVGRVTEAGDGIAR
                     IEGLPSTMANELLRFADGTLGIALNLDVREIGAVILGPYEGIEEGQDVKRTGEILSVP
                     VGDGFLGRVVDALGEPIDGLGDIVQESRRALEIQAPTVVQRQPVKEPLQTGIKAVDAM
                     TAIGRGQRQLIIGDRQTGKTAVAIDTIINQKANWATGDPKQQVKCIYVAIGQKASTIR
                     AVQGALDAAGALEYTTIVAAPASDAAGFKYLAPYTGSAIGQHWMYKGEHVLIVFDDLS
                     KQAEAYRTVSLLLRRPPGREAYPGDVFYLHSRLLERCAKLSDELGGGSMTGLPIIETK
                     GNDVSAYIPTNVISITDGQIFLETDLFNSGVRPAINVGISVSRVGGSAQIKAMRSVAG
                     RLRLDLAQFRELEAFAAFGSDLDAASRSQLERGARLVELLKQAQYSPFPVEHEVVSIW
                     AGTTGQLDEVPVSDIRRFEKEFLEHLRLQHKGILDDIAQTKQLSDATAQSLNEAITSF
                     KKSFETHEGTSLVGNEQPDELDESSMANEKVTKHLPPPEKK"
     gene            6222..7124
                     /locus_tag="VII50_08465"
     CDS             6222..7124
                     /locus_tag="VII50_08465"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HEY5050921.1"
                     /translation="MGAQVRVYRRRIRSVRATAKITRAFELIAASRMIKAQQRVAAST
                     PYAEAITRAVSAVASQSGLDHPLTSKHENATRAAVLLVTSDRGLAGAYSSNVLKEGES
                     LTAFLRDSGFEVLPYIVGRKGLAYYRFRDRFIAGEWSGFSESPSYLNAKDVADTLIAA
                     FNTPVDEGGVDEIHVVYTQFVSMLTQTPVIRRILPLEVEATTDEPAGGPLPSYEFEPS
                     AEGVLDALLPRYVEARVYNALLQGAASELAARRRACKSATDNAEELIKTYTRLANAAR
                     QADITQEISEIVGGADALAATGSE"
     gene            7131..>7337
                     /locus_tag="VII50_08470"
     CDS             7131..>7337
                     /locus_tag="VII50_08470"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719489.1"
                     /note="Produces ATP from ADP in the presence of a proton
                     gradient across the membrane. The beta chain is a
                     regulatory subunit; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HEY5050922.1"
                     /translation="MTTTVDKAAGSDAAHTGIGRVARVIGPVVDVEFGVDDLPEIYNA
                     LEVDIDLSGEDKDVEGLHKHTLTLE"
BASE COUNT         1195 a         2418 c         2411 g         1313 t
ORIGIN      
        1 cccggctgga tcagcctcga acgcctgcgc gagatcgtgc ccggactcga gtcgcgcgcg
       61 tgatgtctcg gtgcgcgcga gccgtagagt aaggctggtc gaaggtcgac cccgccgccg
      121 ccccgaagga gtgacgtgcg cgagtacctg atcacgctat tggtcgcggc gacggtcacg
      181 tacctgtcca cgcccattgc gcggcggttc gcggtgcgcg tcggcgcatt caccggcgtc
      241 cgcgatcgcg acgtccactc gatcccgacc ccacgcctgg gcggtgcggc gatgttcgcc
      301 ggtatggccg ccgcgttact ggtcgcctcc aggatgccga cgctgcaggt cgtcaaccag
      361 cggttctccg agccgcgggc gctgctcatg ggcggtggca tcgtgttcgt gttcggcgct
      421 ctggacgacc gattcaatct ggacgccgtc accaagctcg cgggccagat tctcgccgcg
      481 tcggtcatgg tgttgcaggg cgtacagctg ctgtggttgc cgattccggg ccaaggcacg
      541 ctgattctcg gccaggactg gtcggtcgtg ctcacggtct cgttcatcgt catcgtgatc
      601 aacgccgtca acttcatcga cgggctcgac gggctcgccg cgggcgtcgt cggcatcgcc
      661 gcgctcgcgt tctttgcctt gtcgtacaac ctttccgtgc agtcgcacct gccatacgcg
      721 ctgccgccga tgctgatcgc gatcatcgtg gcgggcatgt gcctcggatt cctgccgcat
      781 aacttccacc cggcgcgggt ctttatgggt gactcgggtt ccatgctcat cggcttgctg
      841 ctcgcggcgt ccaccgtctc gttgaccgga caggtcgacg tcgtcacgca gtaccagcag
      901 tacggcgcgt ttctgccgtt actgctgccg ttcgcgatcc tgctggtgcc gttcgccgac
      961 ctcattctcg cggttgccag gcgcaccaag cagggccgcg cgccgtggtc gcccgacaag
     1021 cagcacctgc accaccggtt actggaactt ggccattctc agcggcgggc ggtgttgatc
     1081 atgtacttct ggtcggcact gttcggcgcc ggcgtggtgg cactgtcggt gaccaagggg
     1141 ccgttggtcg tcgtcgcgct gggtggcgca ctggccgcga tcgcactggt gctggtgaac
     1201 atgccgaggt tgcgggccgc gcagcgttca cgccatggac cggcgtgacc cgggcacgct
     1261 cccagacccg gcagaccgta ggatgacatc cgctcctcag aggccgcagc ttggtctcta
     1321 cgccctgatt ggcattggca ccttgaacct ggtgtcactt ctggtcgggc tggggatcgg
     1381 ttggttcctc gacggtcggt ttcactcgtt ccccgccgcc acgctgatcg gtctggctct
     1441 ggggatcgca gcgggactca ccacgagttg gttgcagatc cgccggtatt tcaccgacgc
     1501 caagtagcac ctgagcaccg tgtcgagggg aggcccaact gatgaacagc accgccccac
     1561 tcatggttcc ggtaggtacc ccgcgtcggg tgttcatcat cgcggccgtt ctcgggggcg
     1621 tcggcctgct gattgcctcg attcttggcc acgtcgtcgt cgggctgctg ctgctcgccg
     1681 ggctcgtgct cggcgccgtc aacaacatcc ttacggtgcg atcgatcttg aagttcacgg
     1741 cggccggtga ccccgaccgc aagaagttcg ccaaggcttc gcttcttcgg ctcgcctaca
     1801 tttccttgat tgccgcgcta ttccttatcg tgttcagacg cgagggcatt gcggtcctgg
     1861 ccgggctcgc ggtgttccac ctgcttgccg cgctcgccac cagcgtgccg gcgctgaagg
     1921 agtttcgtaa ggcatgagct ggtcaagcac cgccccggcg cacgtcgtcg cggcgacgat
     1981 cgacgtcggc gtgcatccgc acgtcaagat tctcgggctc acgatcaact ccgacacggt
     2041 cctgaccacg ctcatcgcgg gtgccatcgt cgtcgggctc ggcctctata tgcgggtgcg
     2101 cgcccaatcc ggagtgccca gcaagctgca gctgatcttc gagctcatcg tcgacaccgt
     2161 gaacaagcag gtcgacgaga gcatgggcat cgaggtcgcc ccattcgtgg tgccgctcgc
     2221 ggtgacgctg ttcctcttca ttctgttgtg caactggatc gggctgatcc cgagcgggca
     2281 cccggagaaa ctgcccgctc cgaccgctga catcaacctc acgctcgcga tggcgttcgc
     2341 cgtgatcatt ccgttgcaca tcgtcagtgt tcggcggcgc gggctgaagg cctacgtcaa
     2401 gcacttcttc cagccctatc cgatcatgtt cccgatcaac ctgatcgagg agatcgtcaa
     2461 gccgttcacg ctggcgctcc ggcttttcgg gaatttgatc tcgggtgcaa tcatggtcgc
     2521 cctgctggcc ctcctgcccg cctacatctt gtgggcaccg caggggattt ggaaggtgat
     2581 cgacgcgttg gtgggtgtca tccaggcctt catcttcgcc ctgctcacga tcctgtactt
     2641 cgcgttcgcc acgaccaccg aaaaccacgg cgccaccgac agtcattctg gaactgatgg
     2701 gcatcccggt accgacgggc atcccgttac cgacgggcat cccgttactg acgggcatct
     2761 cgttcgcgac gggcaacctg ttatcgaagc gcactaacca ctgacaacag ctggaacaag
     2821 gagagacatg gccacggacg ctgcaaacct cgacagcgcc atcaagacag ccggcgcctt
     2881 cgtcggtggc ggcctcgccc tcgccggcgg cgccatcggt gcggccgtcg gtgacggcct
     2941 cgccggcgcc cagacgatcg ccggtgtcgc ccgtcagccc gagtcgcagc cgcggctgat
     3001 gccgatcttc ttcctgacgg tcggtctggt cgaggcgatg tacttcatca acctagcctt
     3061 catggccatg ttcgtcttcg tgctcggcaa gtaggtcgga gcaagcgtgc ccatcttcat
     3121 cgcagccgca agcccatcgc ctgcggcgac gcccgcgaag acgaacaact tcctgatccc
     3181 gaatggcacc ttcatcgccg agctgatcat cttcttgatc atccttgcgg tgatcggcaa
     3241 gtacgtcgtg ccgtttgtga acctgaagtt ggcggagcgg caagaggcga tccggctcga
     3301 gttcaaggag ctcgaggatg cccgggccaa gctcgaggcg accgaggccg agtatcgcga
     3361 cctgatcgcg agcgcccggg ctgacgccgc gcgccagcgc gaggaggctc gcgaagaggg
     3421 cgcccagatt ctggccgacc tgcgcgcgca ggcacaggtc gagtccgagc ggatcaagca
     3481 ggccgcgcat cagcagatcg aggccgagcg gtcgcgcacc gtcgaggcgc tgcgcgccga
     3541 ggtcggcagc ctcgctgtcg acctggccgg ccgggtcgtg ggggagtcgc tgaccgacga
     3601 cgcacgtcaa cggcgggtcg tcgagcggtt cctgattgag gtcgagtcgc gcgccggcga
     3661 agcagccccc ggacaacagg tgcgcggctg atgcagggag ctagtcgcga ggcgctcgcg
     3721 caggcctgga cgtcggtcga gcgccagctc acgccgtcca gtgtcgacgc cgcggtcatt
     3781 ggtgacgagt tgttcggcgt cgccgacgtc atcgacaccc aagtggcgtt gcgtcgtgcg
     3841 ctgtccgacc ccgcgctcga gggcgatcgc aaggcggggc tcatcgaggc cgtgctcggg
     3901 tcgcacgtcg gcgcggtcac cttggcaatc gtgaccgaag tggttcgcgc gcggtggtcg
     3961 cggatgcgcg acctgaccga tgcgatcgag acgctcgccg tcctcgccgt cctgattgcc
     4021 gctgaccgtg ccgggatcac cgacgacgtc gaagacgagc ttttcaggtt cggccgtgtc
     4081 gtggaggcgc gtcctgacct gcgcgatgcg ctgcgcagtc aggccttgcc ggccgcgaac
     4141 aaggtcgcgc tcgtcggggc tttgctcgac gggaaggcca cggtgaccac gacgcggctc
     4201 gtcacgcaga tcgcgctgca cccgcgcggg cgggtgcccg aagacgcgct tgccgagtac
     4261 ggcctggtgg cctcgcgcag gcgcgaccga ttggtcgcgc gggtcgccac tgccattgcg
     4321 ttatcggacg acgaacggtt gcgccttcag gcggcgctgg cggcgatgta cggccacgat
     4381 gtgcatctcg aggtcgagct taacgaggcc gtcgtcggcg gtgtcgttgt gcaggtcggt
     4441 gacgaagttc tcgacgggtc agttgccggt cggctggccg aggcacggcg acgccttgag
     4501 taacccgcgc acctcgacgt ccgcgcacgt cgttctacga aacccacgaa cgaggatgga
     4561 ctgatggcag agctcacgat tcgcccggag gagatccgcg acgcgctcga gcgtttcgtg
     4621 tcgtcgtaca cccccgacgt cgcgtcgcgt gaggaggtcg gccgggtcac cgaagccggc
     4681 gacgggatcg cgcgtatcga gggcctgccg tcgacgatgg ccaacgagtt gctgcgcttc
     4741 gccgacggca cgctcggcat cgcactgaac ctcgacgtcc gcgagatcgg tgcggtcatc
     4801 ctcggaccct acgaaggcat cgaggaaggc caggacgtca agcgcaccgg cgagatcctg
     4861 tccgtgccgg tcggcgacgg cttcttgggc cgagtcgtcg acgcgctggg cgaaccgatc
     4921 gacggactgg gtgacatcgt ccaggagagc cgtcgggccc tcgagatcca ggccccaact
     4981 gtcgtgcagc gccagcccgt gaaggagcca ttgcagacgg gcatcaaggc cgtcgacgcc
     5041 atgaccgcga tcggccgcgg ccagcggcag ctcatcatcg gcgaccgcca gaccggcaag
     5101 actgcggtgg cgatcgacac gatcatcaac cagaaggcga actgggcgac cggcgacccg
     5161 aagcagcagg tgaagtgcat ctacgtcgcg atcggtcaga aggcttcgac gatccgcgcg
     5221 gtgcagggag cgctcgacgc cgcgggcgca ctggagtaca ccacgatcgt cgcggcgccg
     5281 gcgtccgatg ctgcaggctt caagtacctc gcgccctaca ccggctcggc gatcggccag
     5341 cactggatgt acaagggcga gcacgtgctc atcgtgttcg acgatctttc caagcaggcc
     5401 gaggcttacc gcacggtgtc gctgctgctg cgccgtccgc ccggccgtga ggcctacccc
     5461 ggcgacgtct tttacttgca ctcgcggttg ctcgagcgtt gcgcgaagct gtccgacgag
     5521 ttgggcggcg gatcgatgac cgggctgccg atcatcgaga ccaagggcaa cgacgtctcg
     5581 gcgtacatcc cgaccaacgt gatctcgatc accgacggtc agatcttcct cgagaccgac
     5641 ctcttcaact caggcgtccg gccggccatc aacgtcggca tctcggtgtc gcgagtcggt
     5701 ggttccgcgc agatcaaggc gatgcgttcg gtggccggcc ggctccggct cgacctcgcg
     5761 cagttccgcg aactcgaagc ctttgccgca ttcggctccg acctcgacgc ggcatcgcgc
     5821 tcgcaactcg agcgtggcgc ccggcttgtc gaactgctga agcaggcgca gtactcaccg
     5881 ttcccagtcg agcacgaggt ggtctcgatc tgggccggta cgacaggtca gctcgacgaa
     5941 gtaccggtgt ctgacatccg ccggttcgaa aaggagttcc tcgagcacct tcgcctgcag
     6001 cacaagggaa tcctcgacga catcgcgcag accaagcagt tgtccgacgc gaccgcgcag
     6061 agcctgaacg aagcgatcac gagcttcaag aagagcttcg agactcacga aggcacgtcg
     6121 ctcgtcggca acgagcagcc ggacgagctc gacgagtcgt cgatggccaa tgagaaggtc
     6181 accaagcacc tgccaccgcc cgagaagaag tagatcgcga tatgggagct caggtccggg
     6241 tctatcgtcg gcgcatcaga tcggtgcgcg ccacggcgaa gatcacccgc gccttcgaac
     6301 tgatcgcggc gtcccgcatg atcaaggcgc aacaacgggt ggcggcgtcg acgccctacg
     6361 cggaggcgat cacccgcgca gtctccgcgg tggccagcca gtccggtctc gaccaccccc
     6421 tgacgtcgaa gcacgagaac gcgacgaggg ccgccgtcct tctggtcacc agcgaccgcg
     6481 gtcttgcggg tgcgtacagc tcgaacgtgc tgaaggaggg tgagtcgctc accgcgttct
     6541 tgcgagacag cggcttcgag gtgctgccct acattgtggg ccgcaagggg ctggcgtatt
     6601 accgcttccg cgatcgcttc attgccggtg aatggagcgg gttctccgag agtccgagct
     6661 acctaaatgc caaggacgtc gccgacacgt tgatcgccgc gttcaacacg ccggtcgatg
     6721 agggtggtgt cgacgagatc cacgtggtct acacgcagtt cgtctcgatg ctcacccaga
     6781 cgccggtgat ccggaggatc ctgccgctcg aggtcgaagc gacgaccgac gaacccgccg
     6841 gcggaccgct gccgtcgtac gaattcgagc cgagtgcgga gggcgtgctc gacgcactgc
     6901 ttccgcgcta cgtcgaagcg cgcgtctaca acgcgttgtt gcagggcgcg gcatcggagc
     6961 tcgcggcccg tcgtcgcgcg tgcaagtcgg ctactgacaa cgccgaggag ctgatcaaga
     7021 cctacacccg gctggcgaac gcagctcggc aggcggacat cacccaggaa atcagcgaga
     7081 tcgtcggtgg cgctgacgcg ctcgccgcta ccgggagcga gtgagaactc atgacgacaa
     7141 ctgtcgataa ggccgcaggt tctgacgccg cgcacactgg cataggccgc gttgcccgcg
     7201 tcatcggccc ggtagtcgac gtcgagttcg gcgtcgacga cctgccggag atctacaacg
     7261 cgctcgaggt cgacatcgac ctgtcggggg aggacaagga cgtcgagggg ctgcacaagc
     7321 acacgctgac cctcgag
//
LOCUS       DATCBJ010000421         3167 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_485060, whole genome shotgun sequence.
ACCESSION   DATCBJ010000421 DATCBJ010000000
VERSION     DATCBJ010000421.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 3167)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3167)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3167
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_485060"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1394)
                     /locus_tag="VII50_08475"
     CDS             complement(<1..1394)
                     /locus_tag="VII50_08475"
                     /inference="COORDINATES: protein motif:HMM:NF013884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HEY5050923.1"
                     /translation="MEAVAGTECGRIGASRNTYLDLIRVVAIAAVVVGHWIATGITYE
                     HGQLNGIDVLGVSTWTSWLTLIFQVVPLFFLVGGYSNALSWTRYRAAGGSAFGWTRRR
                     VRRLLVPTAGYIAAIALAVVICTGAGADRDDLEEAGWALSLHLWFLAPYLVLLLLTPA
                     LLAAHRRYGVVVPAVMAATAVVIDVGVIEEHWHFVGWANYVLVWGTFHQIGFAWHDGF
                     LAGHRGRPLALGAGAVCALVALIWWGPYPVSMVGVPGARIQNASPPSAALLAFGLAQA
                     GVVLLAAPSVSAWLSRPAHSRLRSLIGRANAMTMPVYLWHMVPVVLVTVVAYPNHWLP
                     QPAIGSRAWWVQRVPWVAALSVVLAAVLALVILAAQTRKPRRPMQPDSNPPSTTRRAA
                     MLGLGVAVTVATLGRLAVWGFAPNGSLDTITIGCFAIGLLLVLAAGAHRPTRDLVGRS
                     VRGESARTSAMAAA"
     gene            1581..>3167
                     /locus_tag="VII50_08480"
     CDS             1581..>3167
                     /locus_tag="VII50_08480"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY5050924.1"
                     /translation="MKTSAQPQAQGTGTKQAKDTEQATDTDTGARGGVKFLLGAMRGH
                     WRAEGSSVAAALICTVAIVAIPELGGRAVDSGLIGHHWRTLGILGGIIAVLGLVQAVT
                     SGLRRYYNGVASRAVEAELRRSFFTRLLGFDVGYHDRVNRGQLLSRVTSDLFQIQALV
                     SSAPIWIANTLTIVAVAVVLVVINPLLGLVAVAGLPIVAYTSLRYSTRVRPILGELQR
                     ERGDLAGVVEEALSGIRAVKGFGAESILDRRLGRQADAVRARSIEIVRMRCRYNPLLN
                     VMPMFELVAVNWFGGYLVLHHELTVGMLLAFNTYLAVVTGPLQAIGWFVVQVQRALVS
                     ARRLETVMSHRVTIREPADPLPLPRGARVGRVSFDDITFGYPGSDEPVLDGFNLEIAS
                     GEVVALVGPTGCGKTTVAALLARLYDPSTGAVRLEGVDLRQLSTDTVRAAVGVVFEDN
                     FLFDDTIAANLRVGCPAATDAELAIAAHVAQADEFVAEMPGGYDSVVGERGLSLSGGQ
                     RQRMALARGILADPRVLVLDD"
BASE COUNT          526 a         1060 c         1081 g          500 t
ORIGIN      
        1 tccgccgcag ccatcgcgct ggtcctcgcc gactcgccac gaactgagcg tcccacgagg
       61 tcgcgggtcg gtcggtgggc accggcggcg aggacaagca acagcccgat cgcaaagcag
      121 ccgatggtga tcgtgtcgag cgaaccgttg ggtgcgaaac cccagacggc cagccgcccg
      181 agcgtcgcca ccgtgaccgc gactccgagc ccgagcatcg ccgcgcgccg ggtcgtgctc
      241 ggcgggtttg agtccggctg catcgggcga cgaggtttgc gggtttgcgc agctaggatc
      301 acgagggcca acactgcggc caagacgacg ctcagcgcgg caacccacgg gacgcgctgc
      361 acccaccagg cgcgggagcc gatcgccggc tgtggcagcc agtggttcgg gtacgcgacg
      421 actgtgacga ggacgaccgg caccatatgc cagaggtaga cgggcatcgt catggcgttg
      481 gcgcggccga tcagactgcg gagtcggctg tgggcgggcc gcgagagcca tgccgagacg
      541 gacggcgcgg cgagcagcac gaccccggcc tgcgccagtc cgaacgcgag gagagcagcc
      601 gagggtggcg atgcgttctg gatgcgtgca ccgggcacgc cgaccatgct gaccggatac
      661 ggcccccacc agatcagtgc caccaaggcg cagacggcgc cggcccctag cgccaacggc
      721 cgccctcggt gtccggctag aaatccgtcg tgccaggcga atccgatctg atggaacgtt
      781 ccccagacga ggacgtagtt ggcccagccc acgaagtgcc agtgctcctc gatcacgccg
      841 acgtcgatga ctaccgctgt tgccgccatg accgcgggca cgacgacgcc gtagcggcgg
      901 tgcgctgcga ggagggccgg tgtcagcaag agcagaacca ggtacggcgc cagaaaccac
      961 aggtgcagcg atagcgccca cccggcctct tcgagatcgt cgcggtcggc accagcacca
     1021 gtacagatca cgaccgcgag tgcgatcgcc gctatgtagc cggccgtggg tacgagcaac
     1081 cgcctcaccc ggcggcgtgt ccagccgaac gcggagccgc cggctgctcg gtacctggtc
     1141 catgagaggg cgttcgaata tccgccgacg aggaagaaga gcggcaccac ctggaagatg
     1201 agggtcagcc acgaggtcca ggtcgagacc ccgagcacat cgatgccatt gagctggccg
     1261 tgctcgtagg tgatgccggt ggcgatccag tggccgacga ccactgccgc gatcgccacc
     1321 acgcgaatca gatcgaggta ggtgttgcgc gacgcgccga tccgaccgca ttcggtgccc
     1381 gcaacggcct ccatgcttct agggtatgcg ccgcgggcga gcagagatca ccgcgaatta
     1441 atgccgcgct gggctagctt tcgcgcggct cgttcgatga tgggcttact gcctgaccaa
     1501 tgcctgagac tctcgagggg gtgcaccggg gaacacacgt tcaccgtgat ggtggcgccc
     1561 cgacggtctt gaccactgtt gtgaaaacaa gtgcacagcc acaagcacaa ggcacgggca
     1621 ccaagcaggc caaagacacc gagcaggcga cagacaccga cactggtgcg cgcgggggtg
     1681 tgaaattcct tctcggggcc atgcgcggcc attggcgagc tgagggcagc tcggttgcag
     1741 cggccctgat ctgtaccgtc gccatcgttg ccatccctga actcggcggc cgcgccgtcg
     1801 acagtggcct catcggtcac cactggcgaa cgctggggat cctcggcggc atcatcgccg
     1861 tgcttggtct ggtgcaggcc gtgacctcag ggctgcggcg ctactacaac ggcgtcgcct
     1921 cgcgcgccgt agaggcggaa cttcggcgga gcttcttcac tcgccttctc ggtttcgacg
     1981 tcgggtacca cgaccgggtg aaccgaggtc agctgctctc tcgcgtcacg agtgacctct
     2041 tccagatcca agctctcgtg tcgtcggccc cgatctggat cgcgaacacc ctcaccatcg
     2101 tcgccgtcgc cgtcgtcctc gtcgtcatca atccgctcct cggcctcgtc gcggtggccg
     2161 gccttccgat tgtggcgtac acgtcgcttc gatactccac gcgcgttcgc ccgatcctcg
     2221 gcgagttgca gcgtgaacga ggcgacctcg cgggcgtcgt cgaggaggca ctcagcggga
     2281 ttcgcgcggt caagggcttc ggcgccgagt cgatcctcga ccgccggctg ggccgccagg
     2341 cggacgccgt gcgcgcccga tcgatcgaga ttgtgcggat gcgctgccgc tacaacccgc
     2401 tgctcaacgt catgccgatg ttcgagctgg tcgcggtgaa ctggttcggg ggctacctcg
     2461 tgctgcacca cgaactcacg gtcggcatgt tgctggcgtt caacacctat ctcgccgtcg
     2521 tgactggacc gctccaggcg atcgggtggt tcgtcgtcca ggtacagcgt gcactggtgt
     2581 cggcgcgtcg gctcgagacg gtgatgagcc accgggtgac gatccgtgag cctgcagacc
     2641 ccttgccact gccgcgcggc gctcgcgtcg gtcgggtctc gttcgacgac atcacattcg
     2701 ggtaccccgg tagcgacgaa cccgtactcg atgggttcaa cctcgagatc gccagcggcg
     2761 aggttgtcgc gctcgttggg ccgacgggtt gcggcaagac cacggtggcc gcgctgctcg
     2821 cacgcctcta cgacccgagc acgggcgcgg tgcgcctcga gggcgtcgac cttcggcagc
     2881 tctcgaccga taccgtgcgc gctgccgtgg gggtcgtgtt cgaggacaac ttccttttcg
     2941 acgacaccat cgcggccaat ctgcgggtgg gctgcccggc cgctaccgac gcggaactgg
     3001 cgatcgcggc gcacgtcgct caggccgacg agttcgtcgc cgagatgccg ggcggctacg
     3061 acagcgtcgt cggtgagcgg gggctgtcgc tgtccggcgg gcagcgccag cggatggcgc
     3121 tcgcacgcgg aatcctcgcg gacccaaggg tgctggtgct cgacgac
//
LOCUS       DATCBJ010000422         7459 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_314637, whole genome shotgun sequence.
ACCESSION   DATCBJ010000422 DATCBJ010000000
VERSION     DATCBJ010000422.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 7459)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7459)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7459
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_314637"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..334
                     /locus_tag="VII50_08485"
     CDS             <1..334
                     /locus_tag="VII50_08485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5050925.1"
                     /translation="VSNGTQLGKKAAEVAGPRPSRATVTGAICEVLDDNGYQPNASPT
                     CITLANCPFHSLAQEYTDLVCGINLDIVSGLLDALDETRWQANLDPAPGRCCVTITRP
                     RGRQAKLS"
     gene            complement(625..1233)
                     /locus_tag="VII50_08490"
     CDS             complement(625..1233)
                     /locus_tag="VII50_08490"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEY5050926.1"
                     /translation="MSALTRWVTDHGPEHSQWYIDRFRAMAADGADLGGEARLLDALL
                     APRSRVLDAGCGPGRVGAELAARGHIVVGVDVDPLLIAAAEADHPGPRWMVDDLAEFD
                     LPAAGVPELFDAAVIAGNVMAYVASGTEPRVLARVAAHVRVGGVVVVGFGTDRGYPIT
                     DFDAHAAAAGLTREHRFATWDLRPWRDDATFAVTVFRKPDQS"
     gene            complement(1359..1907)
                     /locus_tag="VII50_08495"
     CDS             complement(1359..1907)
                     /locus_tag="VII50_08495"
                     /inference="COORDINATES: protein motif:HMM:NF016246.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HEY5050927.1"
                     /translation="MIVYTVGHSTRAIEDFVTLLNAFDIETLVDVRTIARSRHNPQYN
                     EAELDASLRANGISYIRLVGLGGLRHTASASINTAWKNLSFRGYADYMQTPEFLDSLG
                     QLTRIAAGKRTVIMCAEAVPWRCHRSLIGDALLIRGASVEDIFTEKSIRPHRLTAFAR
                     IAGNTISYPAGMAGPCRGPRSA"
     gene            complement(1998..2351)
                     /locus_tag="VII50_08500"
     CDS             complement(1998..2351)
                     /locus_tag="VII50_08500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019511171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEY5050928.1"
                     /translation="MEKFSLTALARQQLEVAKTASSGRASHTVYGGHEHALRQTVIAL
                     AAGQMLDEHENPGEATIHVLTGRVRLAAGDTSWNGSPGDLLVVPNTRHTLEAVEDSAV
                     LLTVAKNLSPQDSST"
     gene            complement(2373..4112)
                     /locus_tag="VII50_08505"
     CDS             complement(2373..4112)
                     /locus_tag="VII50_08505"
                     /inference="COORDINATES: protein motif:HMM:NF012401.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HEY5050929.1"
                     /translation="MSPDTVRSFRNELTVGEDSIDPATWAGSVPVAHGIAPRVRIGRN
                     RWFNLLWLLPIGFVGLIVAVAAAKGLRDTPSVQRFIVRYPGTLPATSASPKPGLPIWV
                     GVQHFFNLFLLIFIIRSGVQILSDHPRLYWTRHSTPGKDWFRIQKPVPADPLWTAKKD
                     SISLPGQVGLPGIRHSIGLARWWHLGTDVLWLLNGLVFYVLLFSTGQWRRLVPTSWKV
                     FPDAVSVVIQYLSLNWPVENGWTAYNAMQLIAYFITVFVAAPLAFLTGLGMSPALSTK
                     FKAISKVLSIQTARSLHFLVLVWFLLFIVIHVTLVFTTGLLRNLNHIFAARNDDSWIG
                     FWIFAASMIVVIVGWVAATPFTMRHPRVVQRVGFALIGPTQRLFEHLDASPGQYTEND
                     ISPYFWHNGQYPDSDEYKALFDSDFAGYRLRVDGLVDNPVELDMAQLRALPYHEQITQ
                     HFCIQGWSGIAKWGGVSMQTIIDVVRPQPAAKWVVFYSLGDGPDKGIYYDAHPIEQMS
                     YHLTMLAYDMNGEPLSFGHGAPLRLRNETQLGFKQVKWIKGVEFVAHYSEIGGGFGGY
                     NQDHEFFGYRQSI"
     gene            4388..6151
                     /locus_tag="VII50_08510"
     CDS             4388..6151
                     /locus_tag="VII50_08510"
                     /inference="COORDINATES: protein motif:HMM:NF014794.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HEY5050930.1"
                     /translation="MKQIAAESLVTRLIDWGVDTIFGLPGDGINGLFEALRRHQDKLK
                     FVLVHHEEAAAFMACAHAKTTGRIGVCIATSGPGGVHLLNGLYDAKLDHMPVLAITGM
                     QETSLLGTGYQQEVHLEKLYENVAEYDVMVTNPLHVTTLVDLAVRHALARRTVSHITF
                     PNDIQVAAADEDPYDHVTPARPPKTSAVFFEAPGRPRETDLRRLAEVLQAGSKIAILA
                     GAGALHARAELLALAELLQAPIVKTLPGKAAVPDDSPFTTGGIGLLGTRPSERLMQQI
                     DTLLMVGTNFPYTKHLPQPDKVRVVQIEADPTRAGNRMPTEAPVIGDAKEALQALLPM
                     LQPRHDSTFLANCQHEMGEWRAEMAALENAERHPIAPQYLMSLINRHASDDAILTCDS
                     GTIATWAARHFDIRGSREFYLSGNLATMAPGLPYAIAAQHAFPDRQVIAFVGDGGFAM
                     LMAEFLTAARHGLPIKVFINNNGAYGQILWEQMVLGYPEFGVRHNGFADFAPWATACG
                     GFGVKVTQPGELEAAVKAALGHPGPALVDCDVNPNEPPMPGIIGKDQALGFGKAFLRG
                     QPHKADIVKTLVADTREKMKS"
     gene            6148..6630
                     /locus_tag="VII50_08515"
     CDS             6148..6630
                     /locus_tag="VII50_08515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5050931.1"
                     /translation="MSAVPRPARRPGSSELVGVDRVIANIRTGHFERTLSALAAVGAA
                     VTTGEIYLSHDSASFGNKMMWLPVVIMPTIIPAGIAGVFSKRAAKTVLPLASAVVVLN
                     GLQGTYLHIRGIAQKPGGLRRGMRLYNAEMGPPVFAPLLASLVGGMGILAAILRREGE
                     "
     gene            6639..7343
                     /locus_tag="VII50_08520"
     CDS             6639..7343
                     /locus_tag="VII50_08520"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025004.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 2-dehydrogenase subunit 3 family
                     protein"
                     /protein_id="HEY5050932.1"
                     /translation="MVFIADRNRGRTPGSSPPRFPGFDVLNEVDRWDAVTAGVVLARL
                     APSPELSFFTGVEQATAEALCDQLLAQHEEPRIPVAALIDVRLATGETDGWHYADMPE
                     DAQAWRQTLAFLDNDAKRQGGTSLSQLGQQQQAALVQAVQELDAQSWHGLPATHVWSL
                     WTRYACTAFYSHPWAWNEIGFPGPAYPRGYKNMGLDAREKFEVADAANTDPVPRTRRA
                     ETAKRRQAAAVTGESS"
BASE COUNT         1391 a         2403 c         2411 g         1254 t
ORIGIN      
        1 ggttagcaac ggcacgcagc tcggcaagaa agccgccgag gtcgccgggc cgcgtcccag
       61 tcgggcgacc gtgacaggcg cgatctgcga ggtactcgat gacaacggtt accagccgaa
      121 cgccagcccg acctgcatca cgctcgccaa ctgccccttc cacagcttgg cgcaggagta
      181 caccgacctg gtgtgcggca tcaaccttga cattgtcagc ggcctactcg acgcactcga
      241 cgaaaccaga tggcaggcca acctcgaccc cgcgccaggc cgctgctgcg tcaccatcac
      301 ccggcctagg ggacggcagg caaaactctc gtagcgtcag ccagttgacc taccactgct
      361 cgctgctcgt tctgccagca agccacccga gcagatcagc atcggtcgag gtcaggcgct
      421 ggacctcctc accaccgtcg catcggcaca gcgcaaccgt gacggacccc tcggtccgtg
      481 acacgacccg ccaggtcgca ccgaaactgt gccaacgctc aaggacctcg accggtcgcg
      541 gctgatccga gcttccaccc gactcggctg gtcgacgcgg ccccgatggt ttggtcaccg
      601 gtccatgatg ccccgaaacg acgatcagga ttgatcgggc ttgcggaaca cggtcacagc
      661 gaacgtcgca tcatcgcgcc acgggcgaag atcccacgtt gcgaaacggt gttctcgcgt
      721 caagccggcc gctgcagcgt gcgcgtcgaa atcggtgatg gggtatccgc ggtccgttcc
      781 gaatccgacc accacgacac cgccgacacg cacatgcgca gcgacgcggg caagtacccg
      841 cggttcggtc cccgaagcga cgtaggccat cacgttcccc gcgatcactg ccgcgtcgaa
      901 tagttcgggc acacccgccg cgggcaggtc gaactcggcc aggtcatcca ccatccaccg
      961 cggccctggg tggtcagcct ccgccgccgc aatcagcaac gggtcaacgt cgaccccgac
     1021 cacgatgtga ccgcgggcgg cgagttcggc gcccacccgc ccgggtccgc atccggcgtc
     1081 caatacgcga gaacgcgggg cgagcagggc gtcgagcaac cgagcctcgc cgcccagatc
     1141 cgcgccgtca gcagccatcg cccggaaccg gtcgatgtac cactgtgaat gttccggacc
     1201 gtgatcagtg acccaccggg taagagcact catgccgtac ccctagtgtc gcgatattga
     1261 agttctttga ccgcaggtag cgcgtggaat gagttcgtcg gccggtttgg tccagacgaa
     1321 cggcacaacc ttcgcgtcgc ccggatcttc ggcctactct acgcagatct gggtccgcga
     1381 cagggtccgg ccatcccagc agggtacgag atcgtgttgc cggctatccg tgcgaacgcg
     1441 gtcaaccggt gcggcctgat tgacttctcc gtgaagatgt cttcgacgga cgcgccccgg
     1501 atcagcaagg cgtcgccgat cagggaccgg tggcagcgcc acgggacggc ctcggcgcac
     1561 ataatgaccg tccgtttccc ggctgcgatg cgggtcaact gcccgagact gtcgagaaac
     1621 tcgggtgtct gcatgtagtc ggcgtagccg cgaaacgaga ggttcttcca ggctgtgttg
     1681 atcgacgcgc tggcggtgtg gcgaagcccg cccaggccga cgaggcgaat ataggagatg
     1741 ccgttcgccc gaaggctcgc gtccagttcg gcttcgttgt attgcgggtt gtggcgagag
     1801 cgggcgatcg tccgaacgtc gacgagggtc tcgatgtcga aggcgttcag cagcgtcacg
     1861 aagtcctcga tcgcccgagt cgagtggccg acggtataaa caatcatctt tccaccaagc
     1921 ctttcagccg aagccgctca tcaggaggag cccgacggaa tcccgaggca tcgatgattc
     1981 ggtttcatgc ccgtccgcta cgtcgatgag tcttgcggcg acaggttctt agcgaccgtg
     2041 aggaggacgg cggaatcctc gacggcttca agggtgtgcc gggtgttggg tacgacaaga
     2101 aggtctccag gcgagccgtt ccaggaggtg tcgccggcgg ctagccgaac ccgtccggtg
     2161 aggacgtgga tggtcgcctc acccgggttc tcgtgttcgt cgagcatctg accggcggcg
     2221 agggcgatca cggtttgtcg cagcgcgtgc tcgtggccgc cgtagacggt gtgcgaggcg
     2281 cggccgctgg acgcggtctt tgccacttcg agttgctggc gtgcaagtgc tgtcaacgag
     2341 aacttctcca tcgagatccc tcctggtcgg tcttagatgg attggcggta gccgaagaac
     2401 tcatggtctt ggttgtagcc gccgaagccg cccccgatct cggagtagtg cgcgacgaac
     2461 tccaccccct tgatccactt gacctgcttg aagccgagtt gcgtttcgtt tcgcaaccgc
     2521 aacggagcac cgtggccgaa cgacaacggc tcaccgttca tgtcgtaggc gagcattgtg
     2581 aggtggtagc tcatctgctc gatcgggtgg gcgtcgtagt agataccttt gtccgggcca
     2641 tcgccgaggg agtagaagac gacccacttc gccgctggtt ggggccgcac cacatcgatg
     2701 atcgtttgca tcgacacgcc accccatttg gcgataccgg accagccttg gatgcagaag
     2761 tgctgggtga tctgttcgtg gtacggcagc gcccgcagtt gcgccatgtc gagttcgaca
     2821 gggttgtcga ccagaccgtc gacgcgtagt cggtagccgg cgaagtcgct gtcgaacaag
     2881 gccttgtact cgtcggagtc tgggtactgg ccgttgtgcc aaaagtaggg cgagatgtcg
     2941 ttctcggtgt actggcccgg gctcgcgtcg aggtgttcga agaggcgctg ggtagggccg
     3001 atgagcgcaa agcctactcg ttgcacgacg cgggggtgcc gcatggtgaa tggggtcgcc
     3061 gcgacccagc cgacgatgac gacgatcatc gacgcggcga agatccaaaa tccgatccag
     3121 ctgtcgtcat ttcgggcggc gaagatgtgg ttgaggttgc ggagcaatcc ggtcgtaaac
     3181 accaacgtga cgtggatgac gatgaaaagc aggaaccaaa ccaataccag gaaatgcaga
     3241 gagcgcgcgg tttgaatgct cagcaccttg ctgatcgcct tgaacttggt cgacagcgcg
     3301 ggtgacatac ccaggccggt gaggaacgcc agcggcgcgg cgacgaagac ggtgatgaag
     3361 taggcgatca gctgcatggc gttgtaggcc gtccagccgt tttccaccgg ccagttcagc
     3421 gacaggtatt ggatcactac cgacaccgcg tcgggaaaca ccttccagct ggtcggcacc
     3481 agccgccgcc attgtccagt cgaaaacagc agaacgtaga agacgagccc attgagaagc
     3541 cacagcacat cggtgcccag atgccaccag cgggcgaggc cgatcgagtg ccgaatccct
     3601 ggcagtccaa cttgcccggg caggctgatg gagtctttct tcgccgtcca cagcggatcg
     3661 gcgggcaccg gcttttggat ccggaaccag tccttgcccg gtgtgctgtg ccgggtccag
     3721 tacagccggg ggtggtccga cagaatctgc acccctgacc ggatgatgaa gatcaacaag
     3781 aacaggttga agaagtgctg cacgccgacc cagatcggca gccccggctt gggtgacgca
     3841 ctcgtcgccg gcaaggtgcc gggatagcgc acgatgaacc gctgcacgga gggcgtatcg
     3901 cgaaggccct tcgcggccgc gaccgcgacg atcagtccga cgaacccgat cggcaacaac
     3961 cacaacaggt tgaaccacct gttgcgcccg attcgaacgc gcggagcgat gccgtgggcc
     4021 accggcaccg agccggccca cgtcgccgga tcaatcgaat cctcccctac cgtcaactcg
     4081 ttccggaagc tgcgcaccgt atccggtgac acgctcgaac ccgccggcac gttctgccta
     4141 gctgggtcgg atcgatccgc accgtgtcgg tcagtggagt ccaccatgtg ccgcaacctt
     4201 cccaaccctg cacagaccga caagggtgcc tagcgatcat gcagctacta ccccgtgcgg
     4261 ctaccggcaa ccggcgggcg cgcctgtgag acagcatcgg gcagagtgta agcccgaaat
     4321 atcgggggac gatacaagga cgggctgcag cgggtgctca gcgccggtca gctgtcgagg
     4381 aggagttgtg aagcagatcg ccgcggagtc attggtgacc cggctcatcg actggggggt
     4441 cgacacgatc ttcggtcttc cgggtgacgg aatcaacggc ttgttcgagg cgctgcgccg
     4501 tcatcaggac aagttgaagt tcgtgctggt gcaccacgag gaggcggcgg cgttcatggc
     4561 gtgcgcgcat gcaaagacca ccggccggat cggcgtctgc atcgccacgt ccgggcctgg
     4621 gggtgtgcat ctgctgaacg gcctgtacga cgcgaagctt gaccatatgc cggtgctggc
     4681 aatcaccggc atgcaggaga cgagcctgct cgggaccggc taccagcagg aggtgcacct
     4741 ggaaaagctg tacgagaacg tcgcggagta cgacgtgatg gtcactaatc cgctacacgt
     4801 cacgaccctt gtcgatctcg ccgtgcggca tgcgctggcg cggcgcacgg ttagccacat
     4861 caccttcccc aacgacatcc aggtcgcggc cgccgatgaa gatccctacg accacgtcac
     4921 tcccgctcgg ccgcccaaga cctcagccgt cttcttcgaa gcgcccggcc ggccgcgtga
     4981 gaccgacttg cgtcgccttg ccgaggtgct ccaggccggc agcaagatcg cgatcttggc
     5041 gggcgccggg gctttgcatg cccgcgccga gctcctcgcg ctcgcagaac tgctgcaggc
     5101 gccgatcgtg aagacattgc ctggtaaggc ggcggtgccc gatgattcgc cgttcaccac
     5161 cggtggcatt ggcctgctcg gcacccgtcc cagcgaacgc ctcatgcagc agatcgacac
     5221 gctgttgatg gtaggcacca acttccccta cacgaagcat cttccgcagc ccgacaaagt
     5281 gcgggtggtg cagatcgaag ccgacccgac ccgagccgga aaccggatgc cgaccgaagc
     5341 acccgtgatc ggggacgcga aagaggcatt gcaggcgctg ctcccgatgc tgcagccgcg
     5401 acatgacagc accttcctgg cgaactgcca gcacgagatg ggcgaatggc gcgccgagat
     5461 ggcggcgctg gagaacgccg aacgccatcc gatcgcaccc cagtatttga tgtcactgat
     5521 caacaggcac gcctccgatg acgcgatctt gacgtgcgac tccgggacga tcgcgacctg
     5581 ggcggcgcgg cacttcgaca tccgggggag ccgggagttc tacctgtccg gcaacctggc
     5641 gacgatggca cccggcctgc cgtacgcgat cgccgcccag catgcctttc ccgatcgcca
     5701 ggtgatcgcg ttcgtcggcg atggcgggtt cgcgatgctg atggcggagt tcctcaccgc
     5761 cgcccgccac gggctaccga tcaaagtctt catcaacaac aacggggcat acggtcagat
     5821 cctgtgggag cagatggtcc ttggctatcc cgagttcggg gtgcgccaca acggcttcgc
     5881 cgacttcgcc ccgtgggcga ccgcgtgcgg cgggttcggg gtcaaggtaa cccaacccgg
     5941 cgagctcgag gcagcagtca aggcggcgtt ggggcacccc ggcccggcgc ttgtggactg
     6001 tgacgtgaac ccgaacgaac cgccgatgcc cggcatcatc ggaaaagatc aagcgctcgg
     6061 gttcggcaag gcgttcctgc gcggtcagcc acacaaggcc gacatcgtca aaacacttgt
     6121 cgccgacacg agggagaaga tgaagtcgtg agcgccgtcc ccaggccggc gcgacgcccc
     6181 ggaagctccg agctcgtcgg ggtcgaccgt gtgatcgcca acatccgcac cggccatttc
     6241 gaacgcaccc tgtcggcgct cgctgccgtc ggcgccgcgg tgacgaccgg tgagatctac
     6301 ctgtcccacg actcggccag tttcggcaac aagatgatgt ggctgccagt ggtcatcatg
     6361 cccacgatca tcccggctgg catcgccgga gttttctcca agcgggcagc gaaaaccgtg
     6421 ctaccgctag cgtcggctgt ggtcgtcctc aacgggttgc agggaacgta cttgcacatc
     6481 cgcgggatcg cccagaagcc gggcgggttg cgccgcggta tgcggctgta caacgccgag
     6541 atgggcccgc cggtgttcgc gccgttgctg gcgagtctgg tcggcgggat ggggatcttg
     6601 gccgcgatcc tgcgccgcga aggcgagtga gcccgagcgt ggtattcatc gctgaccgca
     6661 accgcggccg aacacccggc tcgtcaccgc cccgctttcc tgggttcgat gtgctcaatg
     6721 aggtcgaccg ctgggacgcc gtcacggcgg gcgtcgtgtt ggctcggctc gcaccctcac
     6781 cggagttgtc gttcttcacc ggcgtcgagc aggccaccgc tgaggcgttg tgtgaccagc
     6841 tgctggctca acacgaggag ccgcgcattc cggtggcggc tctgatcgac gtccggctcg
     6901 cgactggtga gaccgacggc tggcactacg ccgacatgcc ggaggacgcg caggcttggc
     6961 ggcagacgct cgcattcctc gacaacgacg cgaagcgcca aggcgggacg tcgttgtcgc
     7021 agctcggtca gcagcaacag gccgccctgg tgcaggcggt gcaagagctc gacgcgcagt
     7081 cctggcatgg gctgccggca acacacgtgt ggtcactgtg gacccggtac gcctgcacgg
     7141 cgttctactc ccacccctgg gcctggaatg agatcggctt cccgggcccg gcctatcccc
     7201 gcggatacaa gaacatggga ctagacgcgc gcgagaagtt cgaagtagcc gatgcggcca
     7261 acaccgatcc ggtgccgcgg acccgacgcg ccgaaacagc caaacgacgg caagccgccg
     7321 cagtgaccgg tgagtcgtcg tgaaactcgg gacgaatctg tcgctgtatg gcgacgtgcg
     7381 ggaccgcaac gagtccgcct ggttgctgcc gagctcaccg ggctttccgc atcggctccg
     7441 tgatgagatg acgcgctac
//
LOCUS       DATCBJ010000423         4261 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_113049, whole genome shotgun sequence.
ACCESSION   DATCBJ010000423 DATCBJ010000000
VERSION     DATCBJ010000423.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 4261)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4261)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4261
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_113049"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..530
                     /locus_tag="VII50_08525"
     CDS             <1..530
                     /locus_tag="VII50_08525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HEY5050933.1"
                     /translation="RRLDVTLDECLEMAAGKTASLMACSASIGAHLTAAPADVVQTLD
                     VFGYELGLAFQLVDDLLGIWGASASTGKPVGADLRARKNSLPVVAAMNSGTAAGAELR
                     SLYGSGALNPDADDDAVARATALVDEAGGRAWAVDESQRRLERACDALSGANLDPPTT
                     ELLIQIAQYVTARDH"
     gene            552..1745
                     /locus_tag="VII50_08530"
     CDS             552..1745
                     /locus_tag="VII50_08530"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEY5050934.1"
                     /translation="MSTGPLITPSSSVSASAQAALAANKAAGPPPAQTVLPRWLVTSG
                     AWSWRLITLGVVGYFVLKFILRIEVVVLPILAAIVFSALLRPLAAFFQRRGLSRLLAT
                     WVAFLIALLLVLGVGTLVIYRATVEWHTLVKDLSATSEKFRHWLSTGPLHLKNQDLAN
                     LQQKAIAQLNAHRGAIANGVVSGASVLVEIAAGIVLTAFITFFFIYDGERIWRFVASP
                     FRARTAERVDRAALAAWNTLSGYIRGSVIIAAIHGLAMGITLSILGVPLVAPLTLLVF
                     ITSFVPLVGVLVGGGLAVFVTMGTEGVTAGVILLAVLVVEHQLEGHLLQPFIMGRYVQ
                     LHPLAIALALTVGTILEGVVGAIVAVPLVATLHAAWPHLRESELVVAPDEPPPPGHVL
                     VDHQP"
     gene            complement(1768..2154)
                     /locus_tag="VII50_08535"
     CDS             complement(1768..2154)
                     /locus_tag="VII50_08535"
                     /inference="COORDINATES: protein motif:HMM:NF024970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY5050935.1"
                     /translation="MEVRTVLPCDASSVAAARRLVRGELTAHPDVPAERTDFVDAASL
                     LVSELVTNAIVHARTDVEVRLVTDDRMLRAEVSDGNPTFPTPRRPIGLAGTGRGLVMM
                     DDLASRWGVSPSGTGKTVWFELTFAS"
     gene            2305..3474
                     /locus_tag="VII50_08540"
     CDS             2305..3474
                     /locus_tag="VII50_08540"
                     /inference="COORDINATES: protein motif:HMM:NF022702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3048 domain-containing protein"
                     /protein_id="HEY5050936.1"
                     /translation="MPQVRARQRHQTLASALAVSAGLLLTACTQASARSGKTGASSSI
                     PASPVGSQAAASSAQALASSAAAAALAAGVSGVAGASPGASSPPPVLSGIDPLTGLPP
                     KSAAAETRPALGVKIDNVQGAWPQAGLNQADIVFDLPVEGGLTRLLAIFHSQDVSLFG
                     PIRSARPADADILHLLGHAYFAFSGGTSSDLGPINDHSNATPMWWDVTPSLFVIRHDH
                     AVPHQVFGTTAMLYAGGEARSPSTTPPPPMFSYQVAVPTAAVPATTVTAQYSAATAAW
                     TWNGTQYLRNQSGHPDLLIDGSQVAATNVVVMSVGVQNTSAHDSHGTVVPLPVVIGSG
                     QVWVFRNGVVVHGTWSRPTENSPMKLQTTTGQVIPLMPGRTWVEVLPNSSLPKIG"
     gene            complement(3479..>4261)
                     /locus_tag="VII50_08545"
     CDS             complement(3479..>4261)
                     /locus_tag="VII50_08545"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEY5050937.1"
                     /translation="SADDLNLGPDTALVCLSHVDYRSSAISDVAGITRAAHDAGALVL
                     WDLCHSVGSVPVTLDADGVDLAVGCTYKYLDGGPGSPAFLYVRKELQATLRQPIWGWF
                     GQRDQFSMGPAYDPAPTIDRFATGTPNIVGLTSVEAGVTLLAAAGIDALRAKGIALAS
                     YLIQLCDAWLVPIGFELASPRDASVRGSHVSLRHPAAYQLCRALIDDHVVPDYREPDL
                     VRCGLSALTTSFADVHEAMRRLRDITSSRRYLDFASQRSRVT"
BASE COUNT          702 a         1460 c         1371 g          728 t
ORIGIN      
        1 agcgtcgact cgacgtaacg ctcgacgagt gcctggagat ggccgcgggc aagaccgcct
       61 ccctcatggc gtgctcggca tccatcgggg cgcacctcac ggccgcgccg gccgatgtcg
      121 tgcagaccct tgacgtgttc ggctacgagc tcgggcttgc gttccaactc gtcgacgacc
      181 tgctcggcat ctggggcgcg tccgccagca ccggaaagcc cgtcggcgcc gacctgcggg
      241 ctcgcaagaa cagcctgccg gtcgtcgcgg cgatgaattc cgggacggcg gcaggtgccg
      301 aattgcgtag cctctacgga tccggggcac tgaacccaga tgctgacgac gatgcggtgg
      361 cacgagcgac cgcactcgtc gatgaggcgg gcggccgggc ctgggccgtc gacgaatcgc
      421 agcggcggct ggagcgagcc tgcgacgcgc tcagcggcgc aaatctcgac ccgcccacga
      481 cggagttgct cattcagatc gcgcagtacg tgaccgccag agatcactag ctgaaggagg
      541 ccgagcagcg catgtcgaca ggaccgctga tcacgccgtc gtcttcggtc tcggcgtcgg
      601 cgcaagccgc gctcgcggcg aacaaggccg cgggcccgcc acctgcgcag accgtgctgc
      661 cacggtggct cgtcaccagc ggcgcgtggt cgtggcggct gatcacgctc ggcgtcgtcg
      721 gctacttcgt gttgaagttc atcctgcgca tcgaggtggt ggtcctcccc atcctcgccg
      781 ccatcgtgtt cagcgccctc ttgcgaccgc tcgcagcatt cttccagcgg cgcggcctgt
      841 cgcggctgct tgcgacatgg gtggcgtttc tgatcgctct gctgcttgtg ctaggcgtcg
      901 gcacactggt catctaccgc gcgaccgtcg agtggcacac cctggtcaaa gacctttcgg
      961 ccaccagcga gaagttccgg cattggctct ccaccggacc gttgcatctg aagaaccagg
     1021 acctcgccaa tcttcagcag aaggcgatcg cccaactcaa tgcgcatcgc ggcgcgatcg
     1081 ccaacggcgt ggtgagcggt gcaagcgtgt tggtggaaat cgccgccggc atcgtcttga
     1141 cggcgttcat cacgttcttc ttcatctacg acggcgagcg gatctggcgt ttcgtagcgt
     1201 cgccgttccg ggcccgcacc gccgagcgtg tcgaccgggc tgcgctcgct gcttggaaca
     1261 cgttgtcggg ctacatccga ggcagcgtga tcatcgcggc gatccacggg ctggcgatgg
     1321 gcatcacctt gtccatcctc ggcgttccgc tcgtcgcgcc gctgacactc ttggtgttca
     1381 tcaccagctt tgtcccactt gttggtgttc tcgtcggtgg cggcttagcg gtgttcgtga
     1441 cgatgggcac tgagggagtc accgcgggcg tcatcctgct tgcggtgctg gtcgtcgaac
     1501 atcaactcga gggacacctt cttcaaccct tcatcatggg ccgctacgtg cagctgcacc
     1561 cactcgcaat cgcgctcgcg ctgaccgtcg ggacgattct cgagggcgtg gtcggcgcga
     1621 tcgttgcggt gccgttggtc gcgactctcc acgcggcgtg gccacacctt cgagagtccg
     1681 aactcgtggt ggcacccgac gaaccgccac cacccggcca cgtgttagtc gatcaccaac
     1741 catgagcagg tggcacgccg gaccgggtca gctggcgaac gtcagctcga accagacggt
     1801 ttttccggtg ccggacggcg aaacgcccca cctgctcgcc aggtcgtcca tcatcaccag
     1861 ccctcggccg gtgccagcca atccgatcgg acgccgtggc gtggggaatg tcggattgcc
     1921 atcgcttact tcggcccgca acatgcggtc gtccgtcacc agccggactt cgacatcagt
     1981 ccgggcatga acgatggcgt tcgtcacgag ctcgctgacc agcaggctgg ccgcgtcgac
     2041 gaaatcggtc cgctccgccg gcacgtccgg gtgggcagtc aattcgcctc ggaccagccg
     2101 ccgtgccgca gcgacgctcg acgcatcgca tgggagcacg gtccggacct ccatgcgaag
     2161 aggatacgag tacgccggct cgagtagggg ccctcgctcc cgggctcgcc gggagaacct
     2221 gacaaccttt gctggtctgc cgacgttttc cggatcggcc aggtagggtc ggccgggttc
     2281 tcatcgatag gaagcaggcg cctcgtgccg caggttcgcg ctcgacagcg gcaccagacg
     2341 ctcgcatcgg cgctcgctgt gagcgccggc ttgctgctaa cggcgtgcac acaggcatcc
     2401 gcgcgatccg gcaagacggg cgcatcctcc tccatcccgg cctcgccggt cggctcacag
     2461 gcggccgcgt cgtccgcgca agcgctggcc tcgtcggccg ctgccgcagc attggcagcg
     2521 ggggtatcag gggtcgccgg cgctagcccg ggggcgagtt ctcctcctcc agtactcagc
     2581 ggcattgacc cactgaccgg cctgccgccg aaaagcgccg ctgcggagac ccgtcccgcg
     2641 ctcggcgtca agatcgacaa tgtccaaggc gcgtggccac aggccgggct gaatcaggcc
     2701 gacatcgtgt tcgacctgcc cgtcgagggc ggccttaccc ggttgctcgc gatcttccac
     2761 tcccaagacg tctcgctgtt cggcccgatt cggtccgcgc ggccggccga tgccgacatc
     2821 ctgcacttgc tcgggcacgc gtacttcgcg ttctcgggtg gcacctcgtc agacctggga
     2881 ccgatcaacg atcactcgaa cgcgacaccc atgtggtggg acgtcacgcc gtcgctgttt
     2941 gtgattcgcc atgaccacgc cgtcccgcat caggtctttg gcaccaccgc aatgctgtat
     3001 gcgggcggcg aggcacgctc gccctcgacc actccgccac cgccgatgtt cagctaccag
     3061 gtcgcggtgc cgaccgccgc ggtgccggct accacggtga ccgcgcaata cagcgcagcg
     3121 acggccgcct ggacctggaa cggcacccag tacctacgca accagagcgg ccatcccgac
     3181 ctcttgatcg acggcagcca ggtggccgca acgaacgtgg tcgtgatgtc ggtcggcgtg
     3241 cagaacacca gcgcgcacga ctcgcacggc accgtcgttc cgctgcctgt ggtgatcgga
     3301 tctggacagg tgtgggtgtt ccgcaacggc gttgtcgttc acggcacatg gagccggccg
     3361 acggagaact cgccgatgaa gttgcagaca accaccgggc aggtcattcc gttgatgccc
     3421 ggccggacct gggtcgaggt gcttccgaac tccagcctgc caaagatcgg ctgaggtcct
     3481 acgtcactct cgatcgctgc gacgcgaagt cgagatagcg acgcgacgat gtgatgtcgc
     3541 gtaaccggcg catcgcctcg tgcacatcgg cgaagctcgt ggtgagtgcc gaaagcccgc
     3601 agcggaccag atcgggctcg cggtagtccg gcactacgtg atcgtcgatg agcgcgcggc
     3661 acaactggta cgccgccgga tggcgcagcg acacgtgtga tccgcgcacg ctggcgtcgc
     3721 gcggactggc aagctcgaag ccgattggca cgagccacgc gtcgcaaagt tggatcaagt
     3781 acgacgccag cgcgatgccc ttggcgcgca acgcatcgat accggccgcc gcaagcaatg
     3841 tcacgccggc ctcgaccgac gtcaggccga cgatgttcgg cgtgcccgtt gcaaaccggt
     3901 cgatcgtcgg cgcaggatcg taggccggac ccatcgaaaa ctgatcacgc tgcccgaacc
     3961 aaccccagat cggctgccgc agtgttgcct gcaattcctt tcggacatag agaaaagcag
     4021 gcgagcccgg gccgccgtcg aggtacttgt aggtgcagcc gaccgcgagg tcgacaccgt
     4081 cggcgtcaag agttaccggc accgagccga ccgagtggca cagatcccac agcaccaacg
     4141 cacccgcgtc gtgcgcagcc ctcgtgatgc cggccacgtc gctaatcgcc gacgaccggt
     4201 agtcgacgtg cgacaggcac acgagcgccg tgtcaggtcc aagattcagg tcgtccgccg
     4261 a
//
LOCUS       DATCBJ010000424         4133 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_396425, whole genome shotgun sequence.
ACCESSION   DATCBJ010000424 DATCBJ010000000
VERSION     DATCBJ010000424.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 4133)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4133)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4133
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_396425"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1135
                     /locus_tag="VII50_08550"
     CDS             <1..1135
                     /locus_tag="VII50_08550"
                     /inference="COORDINATES: protein motif:HMM:NF012933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AlkA N-terminal domain-containing protein"
                     /protein_id="HEY5050938.1"
                     /translation="ARAQRSQTARLLLETTEVPIANVAFASGFASVRQFNDTVREVFA
                     LTPTQLRASRRPASRTPTPGALHLRLPFRRPFAPTPIFEFLATRAVAGVEQGSDSWYR
                     RTVGLTHGSAIIELGDRAGSADAWVKQGWIPCRLELSDLRDLASAVARCRRLLDLDAD
                     AQAVAEALGGDPILGAAICVEPGRRSPGHVDGFELAVRAVLGQQVSVAGARTLAARLV
                     AAHGEPLATANGELTHLFPTAAQVAAADPSTLSLTHARAASLIGLARSVAGGDIDLDP
                     GADRDRTAAQLLALSGIGPWTVSYIRMRALADPDVFMASDLGVRRGLKELGFTGGLID
                     IEKLAQRWRPWRSYALAHIWAAATTAAATTRRPAGRLREEKSA"
     gene            1132..1647
                     /locus_tag="VII50_08555"
     CDS             1132..1647
                     /locus_tag="VII50_08555"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: protein motif:HMM:NF013222.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HEY5050939.1"
                     /translation="MSVQTYFTVLPSPIGELLVVGADDPSAQFAISGLYMTGQRYEPM
                     LQPEWVRDANRFADAEKQLGAYFGGDLTEFDLPLAARGSDFQRSVWTALRDIPLGATC
                     AYGQIAERAADRSKTRAVAAAIGRNPIGIVVPCHRVVGADGSLTGYAGGLDRKRWLLD
                     HEARVSGALLL"
     gene            1674..3671
                     /locus_tag="VII50_08560"
     CDS             1674..3671
                     /locus_tag="VII50_08560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HEY5050940.1"
                     /translation="MANQLAGETSPYLLQHSENPVDWWPWSPAAFDAARARGVAVLLS
                     VGYAACHWCHVMAHESFEDVDTAAQMNAQFVCIKVDREERPDVDAIYMDATQALTGHG
                     GWPMTCFLTPDGQPFYCGTYYPNRPLHGMPSFRQVLDAVSQAWVDRRDELTNAGADVV
                     RLLGERAAMPSEQSPPDVDILEAAVASLRSDFDPEHGGFGGAPKFPPSAVLEFLIRHG
                     SLGALEMVAETCTAMARGGMYDQLAGGFARYSVDNAWVVPHFEKMLYDNAQLARIYAH
                     WWRATGSPLARRVAIETAQFMLTDLRTPEGGFASALDADTNGVEGSTYVWTRGQLLDL
                     LGIDDAQWAADLLLVTAGGTFEHGSSVLQLDPRGPDDQERWSRVRSALLDARRKRPQP
                     GRDDKVVAAWNGLAIAALAEAGVLFDRPDFVDAATDAARLLIDVHLVDGRLRRVSRDG
                     VVGQPAGVLEDYADVAEGFLVLAQVTGSRELLDQAGALLDACLSHFADGSGGFFDTAD
                     DAETLVRRPQNLTDDATPAGQSALAQGLLTYAGLTGSTAHRAAAEAALGATRVLGARY
                     PRAAGWGLAAATALLAGPAEIAIVGTVTGSMTDLERAAWMSTSPGAVIAVGPGHGEPA
                     WAPLLEGRGALDGLPTAYVCRQFVCLRPVTTVKELAAALGG"
     gene            3735..>4133
                     /locus_tag="VII50_08565"
     CDS             3735..>4133
                     /locus_tag="VII50_08565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5050941.1"
                     /translation="MTTENIDEVARGWFAGRLPSDWFVGQPDVTADREEITIVGELAE
                     PDLSAEASEQERAAAWAGRIKAFREETRGQRIEIASEAEHRLGRRIAWGAKCGERREI
                     FTSLSVPVMTRLRQRDRLVLDTLVDAGVARS"
BASE COUNT          624 a         1324 c         1458 g          727 t
ORIGIN      
        1 ggcgcgggca caacgatcgc agaccgcgcg gctgctgctg gaaacaactg aggtgcccat
       61 cgccaacgtg gcgttcgcgt caggcttcgc cagtgtccgc cagttcaacg acacggttcg
      121 cgaggtcttc gcgctcaccc cgacccaact gcgtgcaagc agaagaccag caagccggac
      181 gccgacaccc ggcgcgttgc acttgcggtt gccattccga cggccgttcg ccccgactcc
      241 gatcttcgaa ttcctcgcga ctcgggcggt tgccggagtc gagcagggta gcgactcgtg
      301 gtaccggcga acggtcggtc tgacgcatgg cagcgcgatc atcgaactcg gcgaccgtgc
      361 cggatctgct gatgcatggg tcaagcaggg ttggatacct tgccggctcg aactctccga
      421 cctgcgcgat ctcgcgtccg cagtcgcgcg atgccggcga ttgctcgacc ttgacgccga
      481 cgcgcaggcg gtcgccgagg cgttgggtgg cgacccgatc ctgggtgccg cgatctgcgt
      541 cgagccgggc cgacgcagcc caggtcatgt cgacgggttc gagcttgccg tacgtgccgt
      601 gctcggtcag caggtcagcg tggcaggtgc acgcacgctc gccgcgcgct tggtagcggc
      661 gcacggtgag ccgttagcaa cggcgaatgg tgagctcacc catctcttcc cgacggcggc
      721 gcaggtcgca gccgcagatc cgagcacgtt gtcgctgaca cacgccaggg ccgcatcgct
      781 catcggtctc gcacgctcgg ttgcgggcgg cgacatcgat ctcgacccgg gcgccgatcg
      841 cgaccgcact gccgcgcagt tgcttgcatt atcgggcatc ggaccctgga ccgtcagtta
      901 cataaggatg cgcgcgcttg ccgatccgga cgtgttcatg gcaagtgacc tcggtgtgcg
      961 gcgcggattg aaggagctcg gattcactgg cgggctcatc gatatcgaga aactcgctca
     1021 gcgctggcgg ccgtggcggt cgtacgcgct cgcgcacatc tgggctgcgg caactacagc
     1081 cgctgccacc acccgccgtc cggcggggcg actacgcgag gagaagagcg catgagcgtc
     1141 cagacctact tcacggtgct gccgagcccg atcggtgagc tgttggtggt gggcgcggac
     1201 gacccgtcgg cgcagtttgc gatctccggg ttgtacatga caggccagcg ctacgagccg
     1261 atgttgcagc ccgagtgggt tcgcgacgcc aaccggttcg ctgatgcgga gaagcagctc
     1321 ggcgcgtatt tcggcggtga cctcacggag ttcgaccttc cgttggcggc gcgcggcagt
     1381 gacttccaac gttcggtatg gacggcgctg cgtgacattc cgttgggcgc gacctgcgcg
     1441 tatgggcaaa tcgcggagcg ggcggccgac cggtcgaaga cgcgagcggt cgcggcggcg
     1501 atcggccgta acccgatcgg catcgtggtg ccgtgccatc gcgtcgtcgg cgcggacggc
     1561 tcgctcaccg gctacgcggg cggtctggac cgcaagcgct ggctactcga ccacgaggca
     1621 agggtgtccg gcgcgctgct gctgtaggcg cggggctgcg gttgaatggc atggtggcga
     1681 accagctggc gggtgagacc agtccgtacc tgttgcagca cagtgagaat cctgtcgact
     1741 ggtggccgtg gtcgccagcc gcgttcgacg cagcgcgcgc tcgcggcgtc gcggttctgc
     1801 tctctgtcgg ctacgcggcg tgtcattggt gtcatgtgat ggctcacgag tcgttcgagg
     1861 acgtcgacac tgcggcgcag atgaacgcgc agttcgtctg catcaaggtc gaccgcgagg
     1921 aacgtcccga cgtcgatgcg atctacatgg acgcgacgca agcactgacc ggtcatggtg
     1981 gctggccgat gacgtgtttt ctgacacccg atggccagcc gttctactgc ggtacgtatt
     2041 accccaaccg cccgctgcac ggaatgccgt cgtttcgtca ggtgctcgac gcagtttcgc
     2101 aggcgtgggt cgaccgtcgc gatgagttga ccaatgcggg cgccgacgtc gtgcggttgc
     2161 ttggtgagcg tgcagcaatg ccgagcgagc agtcgcctcc cgacgtcgac atcctcgaag
     2221 cggcggtcgc gtctttgcgt tctgacttcg accccgagca tggcggattc ggcggtgcac
     2281 cgaagttccc gccgtccgcc gtgctcgagt tcttgatccg gcacggatct ctcggtgcac
     2341 tggaaatggt cgccgagacc tgcacggcga tggcgcgcgg cggcatgtac gaccaactgg
     2401 ccggcggctt tgcgcgctac agcgtcgaca acgcgtgggt cgtgccgcac ttcgaaaaga
     2461 tgctgtacga caacgcgcag ctcgcgcgga tctatgcgca ctggtggcgc gcgacaggct
     2521 cgccattagc ccgacgcgtc gcgatcgaga ccgcgcagtt catgctcacg gatctgcgca
     2581 ctcccgaggg tggtttcgct tccgcgttgg acgccgacac caatggcgtc gagggttcga
     2641 catacgtgtg gactcgtggc cagctgctcg atctgctcgg catcgatgac gcgcagtggg
     2701 ccgcggacct cctgctggtc acggcggggg gcaccttcga acacggttct tcggtgttac
     2761 agcttgatcc ccgaggaccg gacgaccagg agcggtggtc gcgggttcgc tcggcgttgc
     2821 tcgatgcacg acgcaagcgt ccgcagcccg gtcgtgacga caaggttgtc gctgcgtgga
     2881 acggtcttgc catcgccgcg cttgccgaag cgggcgtgct tttcgatcgc cccgacttcg
     2941 tcgatgcggc aacggacgcc gcacggttgc tgatcgacgt ccatctcgtt gacggtcgtt
     3001 tgcggagggt gtcgcgcgat ggagtggtcg ggcaaccggc cggcgtgctc gaggactacg
     3061 cggacgtcgc cgaagggttc ctggtgctcg cgcaggtgac cggctcgcgc gagttgctgg
     3121 atcaggcggg cgcgctgctc gacgcctgcc tctcgcattt tgcggacggg tccggcggtt
     3181 tcttcgatac cgcggacgac gccgagacgc tggtgcggcg gccacagaat ctgacggacg
     3241 acgcgacgcc ggccggccag tcggcgcttg cgcaggggtt gctcacctac gccgggttga
     3301 cggggtcgac ggcgcatcgt gccgcggccg aggccgcact cggtgcgacg cgggtgctcg
     3361 gcgccagata tccgcgcgcc gccggttggg gacttgcggc tgccaccgcg ttgctcgcag
     3421 gaccggccga gatcgcgatc gtaggcacgg tgacaggttc catgacggac ctcgaacgcg
     3481 cggcctggat gtccacgtcg cccggtgcgg tcattgcggt cgggccgggt cacggcgaac
     3541 ccgcgtgggc gccgctcctc gagggccgcg gtgcgctcga cgggctgccc acagcctacg
     3601 tctgccggca gttcgtctgc ctgcgtccgg tcaccacggt gaaggagctt gctgccgctc
     3661 tcggcggcta agcggacagc gaacaaaccc caatcggcgt tgtgcttgca ctgtaagcat
     3721 gtaatcatcc gagcatgaca actgagaaca ttgacgaggt cgcccgcggc tggttcgccg
     3781 ggcggctgcc gtccgactgg tttgtaggtc agcccgacgt cacggccgat cgcgaagaaa
     3841 tcaccattgt cggcgaactt gccgagcccg acctctcggc ggaggcgagc gagcaggagc
     3901 gggccgcggc gtgggccggc cgaatcaagg cgttccgcga ggagacccgc gggcagcgca
     3961 tcgagatcgc gtccgaggcg gagcatcgat tgggacgccg gatcgcctgg ggcgcgaagt
     4021 gtggtgagcg ccgcgagatc ttcaccagcc tttcggtgcc ggtgatgacc cgcctgcgcc
     4081 agcgcgatcg cctggtgctc gacaccctgg tcgacgccgg cgtggcacgc agc
//
LOCUS       DATCBJ010000425         4456 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_334809, whole genome shotgun sequence.
ACCESSION   DATCBJ010000425 DATCBJ010000000
VERSION     DATCBJ010000425.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 4456)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4456)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4456
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_334809"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..929)
                     /locus_tag="VII50_08570"
     CDS             complement(<1..929)
                     /locus_tag="VII50_08570"
                     /inference="COORDINATES: protein motif:HMM:NF012296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HEY5050942.1"
                     /translation="MTIDDLPYLNVFSPEYAADPHSVIDPVRAASPLARSERGVEVLA
                     YSDCEDLLRDTGLATGFIEMFEASGISEGPAVDAFKFGIVESEGADHTRLRKAVAPYF
                     TARKIESLRATAHDLVKLWLAETADQGTVDFEAVVGRRLPASLFCMLIGAPLEDSGAM
                     AEYSDGVTRIFQYDPKHRDFIDSRCAELGDYIRRLMGQRRAAPGDDLLSVLVAAQDSG
                     QLTETEVVSMCMVLLGGSTDTTNAQMCMNTLTLARHPEAWALLREHPEHVRAAVAEAV
                     RYAPAMISDVRVARQAAEFSGRQMPQGTMMHAN"
     gene            complement(926..1126)
                     /locus_tag="VII50_08575"
     CDS             complement(926..1126)
                     /locus_tag="VII50_08575"
                     /inference="COORDINATES: protein motif:HMM:NF024851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HEY5050943.1"
                     /translation="MRVVADREACIGATLCSSVSAMFELGRDSKVVVLADGHVGPQDL
                     DAVEEAVEMCPVQALLLTEEPR"
     gene            complement(1129..1503)
                     /locus_tag="VII50_08580"
     CDS             complement(1129..1503)
                     /locus_tag="VII50_08580"
                     /inference="COORDINATES: protein motif:HMM:NF013999.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein"
                     /protein_id="HEY5050944.1"
                     /translation="MPSDQPDAAPSTAQLRDALRAIVDPCSIATGIPINLVDMGLIED
                     VRIENGAVTVTLQLTSPVCMQVTNIRDGAESVLLAVEGVSSVTTVVDPNSAWAPELVD
                     EGARRQLRRLRPLPTYDWKQGR"
     gene            complement(1493..2569)
                     /locus_tag="VII50_08585"
     CDS             complement(1493..2569)
                     /locus_tag="VII50_08585"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEY5050945.1"
                     /translation="MIDGIPVIDGIVHAYNWEPSNYANRHGKMIVDDLGGTFYATTVP
                     GYRLPPTPQAFLRDWPIGEIANISFLESYNDLAVYHVLPINAFKDGSCSLDKGIEATQ
                     RWPDRFIVYAGIDPMQGQKALDDLDEQWERLGHPIGLKLYPNSWVGEQISGWFMNDPE
                     IAFPVFEHARKLGIKIIAVHKALPMGPVEMSHYHTEDIDRAAMAFPDLQFEIVHGGMS
                     FVEETAWQLARFRNVWVNLEVTSSLVTVRPAMLERILAQLISGGPSALDRIIWGTGAV
                     AYHSRPLIEAFVRDFQFSESLLAEGLPELTLNLKAKIMGENYARLAGIDLDDRLTRIK
                     GDEFDTDGDLAPPFSTTGAAGHAE"
     gene            2904..3053
                     /locus_tag="VII50_08590"
                     /pseudo
     CDS             2904..3053
                     /locus_tag="VII50_08590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611217.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(3343..3993)
                     /locus_tag="VII50_08595"
     CDS             complement(3343..3993)
                     /locus_tag="VII50_08595"
                     /inference="COORDINATES: protein motif:HMM:NF024852.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HEY5050946.1"
                     /translation="MTDITIFGGTGYSGGHIATEAAARGHNVLAVSRHAATDPIDGVT
                     YRQGSIHDAGFVDEVASKADTIVIAIRAANSDGPPLLDAIPHICDRACAEGARVGVVG
                     GAGSLHVAEGGALAGDAPGFPDFARAEYLGQSAVLAYLRATAPALDYFYVSPSLEYGA
                     HAPGSARGRYSIGSDILLVDSSGRSAISGADFGLAFVDEIEKPAHRRTRFTVGYTD"
     gene            complement(4089..4313)
                     /locus_tag="VII50_08600"
     CDS             complement(4089..4313)
                     /locus_tag="VII50_08600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5050947.1"
                     /translation="MTPAEFDHLYFEFAQALGAAAEPGLLRDRLILLLLQQVDVATGL
                     DIIGAAAAMPTIADLKQATSDIEPPVTAHH"
BASE COUNT          763 a         1380 c         1529 g          784 t
ORIGIN      
        1 atgttggcgt gcatcatcgt tccttgcggc atctgtctgc cggagaattc ggcggcctgt
       61 cgcgccacgc ggacgtcgga gatcatggcc ggcgcgtagc ggacagcttc ggcgacggcg
      121 gcgcgaacgt gctcggggtg ctcgcggagc agcgcccacg cctcggggtg acgggccagc
      181 gtgagcgtgt tcatgcacat ctgcgcattc gtggtgtctg tgctgccgcc tagcagcacc
      241 atgcacatgc tgacgacctc cgtctcggtg agctgaccgc tgtcctgggc ggccacgagc
      301 accgacagca ggtcgtcgcc gggagccgcc cggcgctgtc ccatgagtcg gcggatgtag
      361 tcgccgagct cggcgcaccg cgagtcgatg aaatcccgat gcttggggtc gtactggaag
      421 atgcgcgtga cgccgtcgga gtactccgcc atcgctccgc tgtcttccag cggagcgccg
      481 atgagcatgc aaaacaggga cgccggcagc cggcgcccca cgacggcttc gaaatcgacg
      541 gtgccctggt cggcggtctc ggccagccaa agcttgacaa ggtcatgcgc ggtcgcacgc
      601 agcgactcga tcttgcgggc ggtgaagtac ggcgccaccg ccttgcgcag gcgcgtgtgg
      661 tctgcgccct cggattcaac gatgccgaac ttgaacgcgt cgaccgcggg accctcgctg
      721 attccggacg cctcgaacat ctcgatgaat ccggtggcca gccccgtgtc gcgaagcagg
      781 tcctcgcagt cggagtaggc aaggacctcc accccccgct cgctgcgcgc gagcggactc
      841 gccgcgcgta ctgggtcgat gaccgagtgc gggtccgcgg cgtactccgg tgagaagaca
      901 ttcaggtagg ggagatcgtc aatggtcatc tcggctcctc ggtgagcagc aatgcttgaa
      961 cagggcacat ctcgacagct tcctcgaccg cgtccaggtc ctgcgggccg acgtggccgt
     1021 ctgcgagcac gacgaccttg ctgtcacggc cgagttcgaa catcgcggac acggagctgc
     1081 acagcgtggc gccgatgcac gcctcacggt cagcgacaac cctcatgctc accgcccctg
     1141 cttccagtcg taagtcggca ggggccggag tcggcgcagc tgccgccggg cgccttcgtc
     1201 gacgagctcg ggcgcccatg cgctgttggg gtcgacgacg gtcgtcaccg acgagacgcc
     1261 ctccaccgcc aagagaacgg actccgcgcc gtcgcggatg ttggtgacct gcatgcagac
     1321 gggactggtc agctgaaggg tgaccgtgac tgcgccgttc tcgatgcgga cgtcttcgat
     1381 cagtcccatg tcgacgaggt tgatcggaat accggtggcg atgctgcacg gatcgacgat
     1441 cgcgcggagt gcgtcgcgca attgtgctgt gctcggtgct gcgtcgggct gatcactcgg
     1501 catggccggc ggcccctgta gtcgaaaatg gcggagccag gtcaccgtca gtgtcgaact
     1561 cgtcgccctt gatccgggtc aggcggtcgt cgaggtcgat gccggccagc cgggcgtagt
     1621 tctcgcccat gatcttggct ttgaggttga gggtcagttc gggcagccct tcggcgagca
     1681 gcgactcgct gaactggaag tcgcgtacga atgcctcgat cagcgggcgc gagtggtagg
     1741 ccaccgcgcc ggttccccag atgatgcggt cgagcgcgct ggggcctccc gagatcagct
     1801 gggcgaggat gcgctccagc atcgccgggc ggacggtcac cagcgagctc gtgacctcga
     1861 ggttgaccca gacgttgcgg aaccgggcaa gctgccacgc ggtttcctca acaaaggaca
     1921 tgcccccgtg cacaatctcg aactgcagat cggggaaggc catcgctgcg cggtcgatat
     1981 cttccgtgtg gtagtgcgac atttcgacgg gccccatcgg cagcgccttg tgcacggcga
     2041 tgatcttgat gcccagcttg cgtgcgtgtt cgaagaccgg gaaggcgatc tcggggtcgt
     2101 tcatgaacca gcccgagatc tgctcgccga cccagctgtt cgggtacagc ttcagcccaa
     2161 tcgggtggcc gagccgctcc cattgctcgt cgaggtcatc gagagctttc tggccctgca
     2221 tcgggtcgat gccggcgtac acgatgaagc ggtccggcca ccgctgggtg gcttcgatgc
     2281 ccttgtccag tgagcacgat ccgtccttga aggcgttgat cggcaggacg tggtagacgg
     2341 cgaggtcgtt gtagctctca aggaacgaga tgttagctat ctcgccgatg ggccaatcgc
     2401 gcaggaaagc ctgcggggtc ggcggaagcc gatatcccgg cacggtcgtg gcgtagaagg
     2461 ttccgccaag gtcgtccacg atcatcttgc cgtggcggtt ggcgtagttg ctcggctccc
     2521 agttgtacgc gtggacaatc ccgtcaatga cgggaatacc gtcgatcaca gagactcctc
     2581 gcagctgtga tagccaatta ctgttgttcg ttatacgaac actatgttcg tataaattag
     2641 acactatgcc gcgcgtcacg tcgagtcaag caatgccgca gtcagctgac tggcgacgtg
     2701 cagacggctg ctgtcgccgt aaccatgtcg tcccgaacgc cggggccgcc tcgcggacta
     2761 ccggtccatc ggcggtgacc accttgaggt tgacgtgacc cccgctggtt gatccggatg
     2821 ctgtgagagc ctcgcgatcc gccacttggg cgggaaggca tgatcgtctt gcccggaggg
     2881 agcggcacac cccttagcag gttgtgcgca agctccagct ggcatcccgg atgcctgccg
     2941 agggcaaggg catcggcgat gtgcgtcggg agcttcaggt ctcggagcag acgtattacc
     3001 gagggctgaa ccagttcggc ggtctgaagg atgatgacgt gaagcggctg aagcacaccc
     3061 gcgagtgccg cagcatcagc ctgttctggt ccctggccca cgcctgggtc cataatcagc
     3121 gactggaaag acgaatagag cgggtctcag gctccgctgc gggccgatcg aggtcatgct
     3181 ggccaccatc gccgtgtcga ttcccgtcgg atggacggta acgacgcgag tgtggcactt
     3241 ggcgagctcg ttggccatgc tcttggccag gcctccgatg ccgtgcttgg cggatgacta
     3301 cgcgaccgag agaggcaggc ctataaatcc ccacggcctc tatcagtcgg tgtacccgac
     3361 ggtgaaccgc gtacggcggt gcgcgggctt ttcgatctcg tcgacgaagg ccaggccgaa
     3421 gtcggccccg gatatcgccg aacgaccgga ggagtcgacg agcaggatat cggagccgat
     3481 cgaatagcgg ccgcgggccg agccgggagc gtgagcgccg tactcgagcg acggactgac
     3541 gtagaagtag tcgagcgcgg gtgctgtggc cctcaggtag gcgagaactg cggattgacc
     3601 gagatactcc gcccgagcga agtcggggaa gccgggagcg tcgccggcca gggcaccccc
     3661 ttcggcgaca tgcaggctgc ccgcaccccc gaccaccccg acgcgggcgc cttcagcgca
     3721 tgcgcggtcg cagatatgcg gaatggcgtc cagcagcggc ggcccgtcgg aattggcggc
     3781 gcggatcgcg atcacgatcg tgtcggcttt gctcgcgacc tcgtcgacga aaccggcgtc
     3841 gtggatcgag ccttgacggt aggtgacgcc gtcgatgggg tcagtggctg cgtgcctgct
     3901 tactgcgagg acgttgtgcc ctcttgcggc agcttcggtg gcaatgtggc ctcccgaata
     3961 cccggtgcca ccgaagatgg tgatgtcggt catgtaaggc tcctagaaga aggttggcaa
     4021 caagaaactc gacagcgagg ctgaggaccc gagccggccg agcggagggg gcagccgagc
     4081 ggatggggtc aatggtgggc ggtgacgggc ggctcgatgt ccgaggtcgc ctgcttcagg
     4141 tcggcgatag tgggcatggc tgccgccgcg cctatgatgt cgagcccggt cgcgacatcg
     4201 acctgctgca ggagcagaag gatcaaccgg tcacgcagca gtcccggctc ggccgccgca
     4261 ccgagcgctt gcgcgaactc gaagtagaga tggtcgaatt cggccggcgt catcgcgcgg
     4321 cctcgccggg caggtccggc attgaggcgt ggacgccgag cactgtggct actgcacgcg
     4381 cgacgtctgt caccgcagga cgtgaccacc ttgcggccac gacggtatcc gcgcgcagca
     4441 gatacaccgc tccgcg
//
LOCUS       DATCBJ010000426         2852 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_371828, whole genome shotgun sequence.
ACCESSION   DATCBJ010000426 DATCBJ010000000
VERSION     DATCBJ010000426.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2852)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2852)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2852
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_371828"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            307..2586
                     /locus_tag="VII50_08605"
     CDS             307..2586
                     /locus_tag="VII50_08605"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding repeat-containing protein"
                     /protein_id="HEY5050948.1"
                     /translation="MSALAVLALGAPLVAVSTSVTTAAGANGVAVTRVSGADRYATAA
                     AIADAGWSAALPANSTLLLATGATFPDAVAGSAAAGHLGVPLLLTASGSLSSSAAAEI
                     DRLKPTRVALLGGTSALNAAVAQQVASHGATVVRWQGSDRYGTAVAVSQATYPSGATN
                     VYLATGTNFPDALAGAGLAAVAGGPLLLTDPKALPSETAAEITRLHPAAIVVLGGTNA
                     VSDQVAAAAVSAAGGATLSRIQGPDRYQTADAVASALVAFKGGTTAGNGVLLATGLDF
                     PDALAGAAWAGTTDRPLLLAPSSYITPQTWQTIQSLAAPNAVVLGGTSAITDAVASGL
                     SSGNPPTSPPAPVLGGATDWPTYHHDAQRTGVATATPAFAGFSAGWKAGLDGAVYGQP
                     IVVGTTVVAATEGGSLYGLSLSTGAVLWRAHIADPIPQSQLPCGDIDPLGITGTPAYD
                     PSDGLVLAVAETAGGHHTLAGVSLATGTIVFHRTLDPLSATAIASQQRGAILVANGRV
                     YVAYGGLAGDCASYIGQVVSVASNGSGTPTGWAVPTTREGGIWAPAGPVEDTDGTILV
                     AAGNGASDTTYDGSDSVTRLSPTLSRLDYFAPSTWANDNDNDLDLGSMSPVLVGGKVL
                     IAGKRGTAYLLNPTHFGGVGGQLSQLGVCQTFGGASVSGNTAYLPCSDGVRAVAVSGN
                     TMTVAWHAASNLTGSPVIASGTVYTTDGSGYLVALNAASGATVGQISVGSLPHFASPS
                     LSGSWALIGTLSGVTAVRT"
     gene            2623..>2852
                     /locus_tag="VII50_08610"
     CDS             2623..>2852
                     /locus_tag="VII50_08610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEY5050949.1"
                     /translation="MTYHAADTRYDQMTYRRCGRSGIDLPAVSLGLWHNFGDDKPVDT
                     QRAILRRAFDLGITHFDLANNYGPPYGGAETN"
BASE COUNT          428 a          966 c          977 g          481 t
ORIGIN      
        1 tgcgctcggt caccgttggc ctggccggat cgatcatttc cgggagtgcc ggcgactggc
       61 gggtcgccga tgtcgacgtc gacccgggct gtgcggcggc tgttgtcgcg gaaggctctg
      121 attggcgggt gcagttcgac cgggtgcagc gccgaactcc gtagggcgcc cgaggttgac
      181 cgcgccgact cccagggtgt ctagggtggt cgaaacggac acactgcggc gattcaccca
      241 acccgaggtg cacctatgcg tgaaagtctc gctccggtcc gcagttgctg ccgccgcact
      301 cactgggtga gcgcgctggc cgtgctcgcg ttgggcgcgc cactcgtcgc cgtctcgacg
      361 agtgtcacga ccgctgccgg cgcgaacggg gtggccgtca cgcgggtatc cggcgctgat
      421 cgctacgcca ccgccgccgc gatcgccgat gcaggctggt cggcggcgct gccggcgaat
      481 agcacgttgc tgcttgcgac cggcgctacg ttccctgatg cagtggccgg ttccgcagca
      541 gcaggacacc tcggcgtccc gctgctgttg actgcgagcg gttcgctgtc gtcgtcggcc
      601 gctgccgaga tcgaccggct caagccgaca cgggttgcgt tgctcggtgg tacgtcggca
      661 ttgaacgccg ccgtcgccca acaggttgcg tcacatggcg caacggtcgt ccgctggcag
      721 ggcagtgacc gctacgggac cgccgtcgcg gtctcgcagg cgacatatcc gagcggggca
      781 accaacgtgt atctcgccac cggaactaat ttccccgatg cactcgcagg tgctgggctt
      841 gctgcggtcg cgggtggtcc gttattgctg accgatccga aggcgttgcc gagtgagacg
      901 gcagccgaaa tcaccaggct gcaccccgcg gcgatcgtcg tgctcggtgg gacgaatgcg
      961 gtcagtgatc aggttgctgc ggccgcggta tcggccgcgg gtggagcgac actgtcgcgc
     1021 attcaggggc ccgaccgtta ccagacggcc gacgcagtcg cgtctgcgct cgtcgcgttc
     1081 aagggcggga cgacagcggg caacggcgtg ctgctcgcga ccggtctcga ctttcctgac
     1141 gcacttgcag gcgccgcgtg ggctggcacg actgataggc cgctgctgct agcgccgtcg
     1201 tcctacatca cgccgcagac ctggcagacg atccagtcgc ttgccgcacc caacgcggtc
     1261 gtgctcggcg gcacgagcgc catcacggac gctgtcgcgt cagggctcag ctcgggtaac
     1321 ccgccgacct cgccacccgc gccggtattg ggcggggcga ccgactggcc cacctaccac
     1381 cacgatgcgc aacgcaccgg agttgcgacg gcgacgccag cgttcgccgg tttctccgca
     1441 ggctggaaag cgggattgga cggcgcggtg tacggccaac cgatcgtggt cggaacgaca
     1501 gtcgtcgccg cgacggaggg cggctcgctt tacgggttgt cgctgtcgac cggtgcagtg
     1561 ctctggcggg cgcacatcgc tgatccgatc ccgcaatcgc agcttccgtg cggtgacatc
     1621 gatccgctcg gcatcaccgg caccccggcg tacgatccga gtgacggact cgtgctagcc
     1681 gttgccgaaa ctgccggcgg tcatcacacc ctcgcgggcg tgagcctcgc gaccggcacg
     1741 atcgtcttcc accgcacgct cgacccgttg tcggccaccg cgatcgcgag ccagcagcgc
     1801 ggcgcgatcc tggtcgcgaa cggccgcgtg tacgtcgcct acggcggcct tgcgggcgac
     1861 tgcgcgagct acatcggcca ggtcgtctcg gtcgcaagca acggaagcgg gacgccgact
     1921 ggctgggcgg tgccgaccac tcgcgaaggc ggcatctggg cgcccgcggg accggtcgaa
     1981 gacaccgatg gcacgatcct cgtggccgcc gggaacggcg cctcggacac gacctacgac
     2041 gggtcggact cggtgacgcg gctctcgccg accctgagcc gccttgatta cttcgcgccg
     2101 agcacgtggg caaacgacaa cgacaatgac ctcgatctgg gctcgatgtc gcccgtgctc
     2161 gtcggcggca aagtgctcat cgcgggcaag cgcgggacgg cctacctgct caacccgact
     2221 cacttcgggg gagtcggcgg ccagctgtcc cagctcggcg tctgccagac cttcggtgga
     2281 gcttcggtct cgggcaacac cgcctacctg ccgtgcagcg acggcgtccg ggcagtcgcg
     2341 gtctcgggca acacgatgac tgtcgcgtgg cacgctgcgt cgaatctgac cggatcgccg
     2401 gtgatcgcgt cgggcacggt gtacacgact gacggctcgg ggtatctggt agcgctgaat
     2461 gcagcgtcgg gggccaccgt tgggcagatc tctgtcgggt cgcttccgca cttcgcatca
     2521 cccagcctgt cgggcagctg ggcgctgatc ggtaccctta gcggcgtcac cgctgttcga
     2581 acttgacccc tcttcggatc tgatcaagcc tgggagcacg tcttgacgta ccacgctgcg
     2641 gatacccgct acgaccagat gacctaccgg cgatgcggac gtagtgggat cgacttgcca
     2701 gcggtatcgc tgggcctgtg gcacaacttc ggcgacgaca agcctgtcga cacgcagcgc
     2761 gcgatcctgc gccgcgcgtt cgacctgggg atcacccact tcgatctcgc gaacaactac
     2821 gggccgccct acggcggtgc ggagacgaac tt
//
LOCUS       DATCBJ010000427         7142 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_150827, whole genome shotgun sequence.
ACCESSION   DATCBJ010000427 DATCBJ010000000
VERSION     DATCBJ010000427.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 7142)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7142)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7142
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_150827"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..264)
                     /locus_tag="VII50_08615"
     CDS             complement(<1..264)
                     /locus_tag="VII50_08615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5050950.1"
                     /translation="MSTSTSDPTERSESPLLEMLGVAEDPMGAADPVSFLRSLAAMGP
                     ALLRNPAGVAAANARLAVGWASAAAATIARTFGVDAPGPVSPEV"
     gene            complement(327..689)
                     /locus_tag="VII50_08620"
     CDS             complement(327..689)
                     /locus_tag="VII50_08620"
                     /inference="COORDINATES: protein motif:HMM:NF024252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEY5050951.1"
                     /translation="MISQRDQLRMLGEFIRAQRQMANLSLRQLSALAEVSNPYLSQLE
                     RGQHAPSVRVLRSIAEALDVSAESLLTQVGLIDAAAHVDDDVTESAIRCDDRLSESQK
                     RALCNVYRSFVEAHATDV"
     gene            complement(733..1041)
                     /locus_tag="VII50_08625"
     CDS             complement(733..1041)
                     /locus_tag="VII50_08625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5050952.1"
                     /translation="MATTNAKTAHSSELPEIAAQVREQLVSTVKQGQKLTVDAVQAWT
                     KAAAALPVPELPQIPGVASLRDVEAITAYTFDLAIELLNAQRDFAVQLAVAFAPTKAA
                     "
     gene            complement(1321..6543)
                     /locus_tag="VII50_08630"
     CDS             complement(1321..6543)
                     /locus_tag="VII50_08630"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin-dependent
                     ribonucleoside-diphosphate reductase"
                     /protein_id="HEY5050953.1"
                     /translation="MTETTRDTQSDPITTGTRTSGRGLSIERVYTTPGVHPYDEVTWE
                     RRDVVMTNWRDGTINFEQRGVEFPEFWSVNAANIVTTKYFRGAVGTDQREWSLKQLID
                     RVVLTYVKAGQVNGYFATVDDATIFEHELTYALLHQVFSFNSPVWFNVGTSAPQQVSA
                     CQPYDALVNTPAGLVPIGKLVEDNAVGTKVFDAHGLTRIVATKSNGVKEVLRLHTKAG
                     HQLDVTADHLVWRCSSETSGRFVAAGELKPGDKLQWHRRDASGEAEITSLDIAEAALA
                     GWLQSDGFVGQYTSGTNKSLTIEAMSVTPAELAWVTAAMDQVFPNAHSHERSVVTIDT
                     SRDCRRTRMCGRHLAPFVDKWGLRARGVDMVVPPALFTAPLPVVAAYLRSIFQAEGYV
                     SARAASTIVAVDMVSEELVRGLQALLSRFGIFARVSPKEDPRPNRLGMWIVHLQNAGD
                     RAVFEAEIGFIDPIKAEKLANSFGMPGRPALDTKRLEIGSVESLGAMEVYDIQTESGE
                     YLSNSLRVHNCFILAVDDKMDSILDWYKEEGMIFKGGSGSGVNLSRIRSSKELLSSGG
                     TASGPVSFMRGADASAGTIKSGGATRRAAKMVILDVDHPDIEEFIETKAREEDKIRVL
                     RDAGYDMDLGGRDMSSVQYQNANNSVRVNEEFMRAYEQGKDFGLKARTDGSVIETVDA
                     KELMRKMAQAAWECADPGIQYDDTINDWHTCPETGRITASNPCSEYVHLDNSSCNLAS
                     LNLMKFLKDDDSFDAVTFAKVVEFVITAMDISICFADFPTEKIGDTTRKFRQLGIGYA
                     NLGALLMATGHAYDSDSGRALAAGITSLMTGTAYKRSAELAGIVGPYEGYARNAEAHT
                     RVMRKHAAASDSIRSVGIIDREVIRMANKAWQDGLQIGAQNGWRNSQASVLAPTGCLT
                     GDTIITTDRGLTRLSELGDVYGDRWQDVDFQVSTDEGSRDATKFFVNGEEPTRRIVTE
                     GGYSVQGTLAHRVKVVNPQTGEWAWKRLVDIEVGDLVPMQLGTMIGEPRVVPLPVLDQ
                     AYYAGDRSLHVPDAVDAELAELVGYFMGDGSLHAKGIRLCVANTDLDVVDRMRILSKS
                     LFGLEPVVTQQEGYQAVMLQSVRLARWWQAAGFAKTLPNADHVGKGWTPRVPSALLET
                     NDVNVYAAFVRGLFEADGTVQSGVPSVSTSSASFAAEIRTLLLAIGLPTTTRETVSGF
                     GGPIFQVRVRNLDHAAAYGEGVGFISERKARLIEDVTASQSGNRDRIHLPRVLWDTLA
                     PIGSDLRSVIVSSLGKSGGVSRTTARHLLVETHDDRLAHALDYIYEEVAANDDGGIQP
                     TYDLSVPSNVTYVAAGFVSHNTIGLMMDCDTTGIEPDLALVKFKKLVGGGSMQIVNQT
                     VPRALRNLGYQAEQVEAIIEHIAEHGNVIDAPGLKPEHYEVFDCAMGERSISAMGHVR
                     MMAAVQPALSGAISKTVNLPENATVEDVEDVYYQGWKLGLKALAIYRNNCKVGQPLSD
                     AREKKTEAVVTASGEYKPVRKRLPKRRPSQTVSFAVAGAEGYLTAGSYPDDGLGEVFL
                     KLGKQGSTLAGVMDAFSIAISIALQYGVPLEAFVSKFVNMRFDPAGLTDDPDVRMAQS
                     IMDYIFRRLALDYLSYDARADFGIFTADERAQTVASKYGDDTGAPVIDDELEYVDSIS
                     QSAPIEKPSTVTLAEVASAKPVDAEVHSSTELLERQLGTAADAPLCMGCGTKMRPAGS
                     CYVCEGCGSTSGCS"
     gene            complement(6621..>7142)
                     /gene="nrdR"
                     /locus_tag="VII50_08635"
     CDS             complement(6621..>7142)
                     /gene="nrdR"
                     /locus_tag="VII50_08635"
                     /inference="COORDINATES: protein motif:HMM:TIGR00244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HEY5050954.1"
                     /translation="SVRLTRCGSGGATVFCPFCHHTDSRVVDSRVADDGTAIRRRRSC
                     PECGRRFTTLETASIAVVKRSGVTEPFSRAKVVAGVRKACQGRPVDEDALAFLAQRVE
                     DDLRANGCSEISSQDVGLAVLAPLRDLDDVAYVRFSSVYRGFDSLEAFESEIALLRAE
                     RDLQRGQALPAKP"
BASE COUNT         1295 a         2263 c         2252 g         1332 t
ORIGIN      
        1 cacctcgggc gagacggggc ccggcgcgtc gacgccgaac gtgcgcgcga tcgtcgccgc
       61 ggcggccgac gcccacccaa ccgccagtct cgcgttcgct gctgcgacac ctgcggggtt
      121 tcgcagcagc gcagggccca tcgcggccaa cgatcgcagg aacgacacgg gatcggccgc
      181 ccccatcgga tcctcggcga caccgagcat ctcaagtaac ggactctcgc tgcgctcggt
      241 cgggtctgat gtggacgtcg acataggtgc tcctcgttag cgccggttcg acccgccgag
      301 gtagctcggg ccggcaacgg ctgggatcag acgtccgtgg cgtgagcctc gacgaagctg
      361 cgatagacgt tgcacagcgc acgcttttgc gactcgctca accgatcatc gcaccggatc
      421 gccgactcgg tgacgtcatc gtcgacgtgc gcggccgcgt cgatgagacc aacctgcgtg
      481 agtagcgact cggccgagac gtccagcgcc tcggcgatgg agcgcaacac gcgcacggaa
      541 ggcgcatgct gcccgcgctc gagttggctc aggtatgggt tggacacctc ggcgagcgca
      601 gaaagctggc gcagcgacag atttgccatc tgtcgctgcg cccgaatgaa ctcgccgagc
      661 atgcgcagct gatcacgctg tgagatcaca gattcctcca gcgccgatta gctgcgggtg
      721 cgaccaacgc gctcaagcag ccttggtcgg cgcgaacgcg accgcgagct gaacggcgaa
      781 gtcgcgctga gcattgagaa gctcgatcgc gaggtcgaac gtgtaagcgg tgatcgcctc
      841 gacatcgcgc aacgatgcga cgccggggat ctgcgggagt tccggcaccg gcagcgcggc
      901 cgcggcctta gtccacgcct ggacggcgtc gacggtcagc ttctgaccct gcttgacggt
      961 tgaaaccaac tgctcacgaa cttgcgcggc gatctctgga agctctgacg agtgggcggt
     1021 cttcgcattg gtggtagcca tggggtgctc cttcttcgtg ttgcggtctg acagaccttc
     1081 gtgttgttcc gggtctggcg tacgtgctta ggatacaagt catctgctag atatgtcaag
     1141 cgagcacact gggcaggcgc cgcgatgctg gcaagtcaag gctgcgtgcc cgaccgcttt
     1201 gcgcaccgcg ggactggtgc acgactggcc agaagttgcc aacctcaaga cacgcgtccg
     1261 ctgaccgaac tgaggacgtt acgtgcaccg ccaaacgcga accttgaatg gccgttgacg
     1321 ctagctgcag ccgctggtgc ttccgcagcc ttcgcagacg tagcagctac cggccgggcg
     1381 catcttcgtg ccacacccca tgcagagcgg cgcgtcggct gccgtaccca gttgcctctc
     1441 cagcaattcc gtcgaactgt gcacctcggc gtccaccggc ttggcacttg cgacctcagc
     1501 caacgtgacg gttgacggct tctcgatcgg cgctgactgc gagatcgagt cgacgtactc
     1561 gagctcatcg tcgatgacgg gagcgccggt gtcgtcgcca tacttcgacg cgactgtctg
     1621 cgcccgctcg tcggcggtga agatgccgaa gtcagcacgt gcgtcatacg acaagtagtc
     1681 gagggcgagc ctgcggaaga tgtagtccat gatcgactgc gccatccgca cgtccgggtc
     1741 gtcggtcaag ccggccggat cgaagcgcat gttgacgaac ttcgacacga aggcctcgag
     1801 cgggactccg tactgcagcg cgatcgagat ggcgatggag aacgcgtcca tcacgccggc
     1861 cagcgtcgag ccctgcttgc cgagcttgag gaacacttcg ccgagaccgt cgtccgggta
     1921 ggagccggcc gtcaggtaac cctcagcgcc cgcgactgcg aacgacacgg tctgcgacgg
     1981 acggcgcttc ggcagccgct tgcgaaccgg cttgtattcg cccgatgctg ttacgacggc
     2041 ttccgtcttc ttctcgcgcg cgtcggacag tggctggccg accttgcagt tgttgcggta
     2101 gatcgcgagt gccttcaggc cgagcttcca gccctggtag taaacgtcct ctacgtcctc
     2161 aactgtggca ttttctggca aattgacagt ttttgaaatg gctccactga gcgccggttg
     2221 tacggccgcc atcattcgca cgtggcccat cgcggagatc gaacgctccc ccatcgcgca
     2281 gtcgaaaacc tcgtagtgct ccggcttcag gccgggcgcg tcgatgacgt tgccgtgttc
     2341 ggcgatgtgc tcgatgatcg cctcgacctg ctccgcttga tatccgagat tgcgcagagc
     2401 gcgcggcacc gtctggttga cgatctgcat cgaaccgccg ccaacgagct tcttgaactt
     2461 gaccaacgcg aggtctggct cgatgccggt ggtgtcgcaa tccatcatca agccgatggt
     2521 gttgtgactg acaaacccgg ccgccacata ggtgacgttc gacgggactg ataggtcgta
     2581 tgtcggctgg atgccgccat cgtcgttggc agcgacttcc tcgtagatgt agtcgagtgc
     2641 gtgagccagc cgatcgtcat gagtttccac gaggagatgc cgggcagtcg tgcgagatac
     2701 gccaccgctc ttcccgagcg acgaaactat gacggagcgg aggtcagaac cgatcggtgc
     2761 gagcgtgtcc cacaatacgc gcggcagatg aatgcgatca cggttgccgg attgcgacgc
     2821 agtgacatcc tcgatcagac gagccttgcg ctcgctgatg aaaccgaccc cttcgccgta
     2881 tgcagcagcg tggtcgagat tgcgcacccg cacttggaag attgggccgc cgaagccgct
     2941 cacggtctcg cgcgtcgtcg tgggcaatcc gattgcgagc agcaacgtgc ggatctccgc
     3001 agcaaagctg gccgatgatg tcgacactga gggaacgccc gactgaaccg tgccgtctgc
     3061 ctcgaacagc ccacgcacga acgcggcgta gacattgacg tcgtttgtct cgagcaatgc
     3121 ggacggcacg cgcggcgtcc agcccttgcc gacgtggtcg gcgttcggca acgtcttcgc
     3181 gaagccagcg gcctgccacc agcgcgcgag acgcaccgac tgcagcatca ccgcttggta
     3241 accctcttgc tgggtgacga ctggctccag gccgaagagg ctcttgctga ggatgcgcat
     3301 ccggtcaaca acatcgaggt cggtgttggc gacgcagagc cgaattccct tggcgtgcag
     3361 gcttccgtca cccataaagt aaccgacgag ctcggcgagc tcagcatcga ccgcgtccgg
     3421 gacatgcagg ctgcggtcgc ccgcgtagta cgcctgatcg agcaccggaa gtggcacgac
     3481 gcgaggctcg ccgatcatgg tgccgagctg catcgggacg aggtctccaa cctcgatgtc
     3541 cacgaggcgc ttccaggccc attcgcccgt ctgaggattg accaccttga cgcggtgggc
     3601 gagcgtgccc tgcacgctgt agccgccctc agtcacgatc cgacgagtgg gctcctcacc
     3661 gttcacgaag aacttggttg cgtcacgcga gccttcgtcg gtcgaaacct ggaagtcgac
     3721 gtcctgccag cgatcgccat agacgtcgcc caattccgac aaccgggtga gcccacggtc
     3781 agtcgtgatg atcgtgtcgc cggtgaggca gccagtgggt gcgagtactg aagcttgcga
     3841 gttgcgccaa ccgttctgcg cgccgatctg cagaccgtcc tgccacgcct tgttggccat
     3901 ccggataact tcgcgatcga tgatgccaac gctgcggatc gagtcagatg cggcggcgtg
     3961 cttgcgcatg acacgtgtgt gcgcctcggc gttgcgcgcg tagccctcgt acggaccgac
     4021 gatcccggcc agctcagccg accgcttata tgcggtaccc gtcatcagag aagtgatgcc
     4081 tgctgcaagt gcccgaccgc tgtcggagtc atacgcgtgg cccgtcgcca tcaacagcgc
     4141 acctaggttc gcgtagccga taccgagctg gcggaacttg cgagtagtgt caccgatctt
     4201 ctcggtcggg aagtcggcga agcagatcga gatgtccatc gcagtgatga cgaactcgac
     4261 gaccttggcg aaggtaacgg cgtcgaagct gtcgtcgtcc ttcaagaact tcattaagtt
     4321 gagagatgcg aggttacagc ttgaattgtc caaatgtacg tattctgagc agggattact
     4381 tgcggtgatt cgtccggttt ccgggcaggt gtgccagtcg ttgattgtgt cgtcgtactg
     4441 gatgccgggg tcggcgcatt cccacgcagc ctgagccatc ttccgcatga gttccttggc
     4501 gtcgacggtc tcgatgaccg agccatcggt gcgggccttg agcccgaagt ccttgccctg
     4561 ctcgtaggcg cgcatgaact cttcgttcac tcgcaccgag ttgttggcgt tctggtactg
     4621 cacgctggac atgtcgcgac cgccgaggtc catgtcgtag ccggcgtcgc gcagcacgcg
     4681 gatcttgtct tcctcgcgtg ccttggtctc gatgaactct tcgatgtcag ggtggtcgac
     4741 gtcgaggatc accatcttcg ccgcgcgacg agtcgcacca cctgacttga tcgtgccggc
     4801 gctcgcatcg gcgccacgca tgaacgacac cgggccggaa gcggtgccac cagacgacag
     4861 cagttccttc gacgaacgga tgcgagagag gttgactcca gagcccgaac cgcccttgaa
     4921 gatcatcccc tcttccttgt accagtcgag gatcgaatcc atcttgtcgt cgacggcgag
     4981 gatgaaacag ttgtgcacgc gaaggctgtt cgagaggtac tcaccgctct cggtctggat
     5041 gtcgtaaacc tccatcgcgc caagcgactc gacggagccg atctcaaggc gcttggtgtc
     5101 cagtgccggc cgaccgggca tgccgaaact gttggcaagc ttctctgcct tgatcgggtc
     5161 aatgaacccg atctcggcct cgaagacggc gcgatcgccc gcgttttgga gatgaacgat
     5221 ccacatgccg agccgattcg gtcgcgggtc ctctttgggt gatactcgag cgaaaatccc
     5281 gaacctcgag agcaacgcct gcaacccgcg cacgagctcc tcggagacca tgtcgacggc
     5341 aacgatggtc gaagctgcgc gtgccgacac atagccctcg gcttggaaga tgctccgcag
     5401 gtacgcagcg accactggca acggtgccgt gaacaacgcc ggcggcacaa ccatgtcgac
     5461 gcctcgcgcg cgcagccccc acttgtcgac gaacggcgcg aggtgacggc cacacatacg
     5521 cgtacggcga cagtcgcgag acgtgtcgat cgtgacaaca ctgcgctcat ggctgtgcgc
     5581 gttggggaag acttggtcca tcgcggcggt cacccaggcc aactccgccg gcgtcacaga
     5641 catggcctcg atggtgagcg acttgttggt gccactcgtg tactgaccga cgaacccatc
     5701 ggactgcagc cagccagcga gggccgcctc ggcaatgtcg agagaggtga tctcggcctc
     5761 gccagatgcg tcccgccggt gccactggag cttgtctccc ggcttaagct cgcccgcggc
     5821 aacaaatcgt ccgctggtct cgctactgca gcgccacaca aggtgatcgg ccgtaacgtc
     5881 gagttggtgt ccggccttgg tgtgaagccg caggacttcc ttaacgccgt tggacttcgt
     5941 ggcgacgata cgcgtcagcc cgtgagcgtc aaagaccttc gttccgacgg cgttgtcctc
     6001 gacgagcttg ccaatcggca caagcccggc cggggtattt acgagtgcgt cataaggctg
     6061 gcacgcagac acctgctgcg gagccgacgt cccgacgtta aaccagacag gcgaattgaa
     6121 gctgaatacc tggtgcagca aggcataggt gagctcgtgc tcgaagatcg tggcgtcgtc
     6181 gacggtggcg aagtagccgt tgacctgccc ggccttcacg taggtaagca cgacccggtc
     6241 gatgagctgc ttgagcgacc actcgcgctg atcggtgccg acggccccgc ggaagtactt
     6301 ggtggtgacg atgttggcgg cgttgaccga ccagaactcg gggaactcga ccccccgctg
     6361 ttcgaagttg atcgtgccgt cacgccagtt cgtcatgacg acgtcgcgac gctcccaggt
     6421 gacttcgtcg taggggtgca cgccgggcgt ggtgtaaacc cgctcgatgc tcaaaccccg
     6481 cccagaagtc cgcgtgcccg tcgtaatcgg gtcgctttgg gtatcacgtg tcgtctctgt
     6541 catgcgtatc tcctccagtg tctacaacac cactaccggc tgccggattt cccccggccg
     6601 ggatcgtttt tggttgcgcg ttagggcttt gcgggcagcg cttgcccccg ctgcaggtca
     6661 cgttcagctc gaagcagcgc gatctcggac tcgaacgcct cgagtgaatc gaagccccgg
     6721 tagaccgaag aaaaccgcac gtaggcgacg tcatcgaggt cgcgcaaagg cgcgaggacg
     6781 gcgaggccga cgtcctggct cgagatctcg ctacagccgt tggcccgcag gtcgtcttcc
     6841 acccgctggg cgaggaatgc gagcgcgtct tcgtcgaccg gtcgcccctg gcacgccttg
     6901 cggacgccgg cgacgacctt ggctcgcgag aagggttcgg tcacacccga ccgcttgacg
     6961 acggcgatcg aggcggtctc cagggtggtg aaccggcggc cacactcggg gcaggatcgc
     7021 cgacgacgga tagcggttcc gtcgtcggcg acccggctgt ccacgacccg gctgtcggtg
     7081 tggtggcaaa agggacaaaa cactgtcgcc cctccacttc cacaccgagt cagccggacc
     7141 ga
//
LOCUS       DATCBJ010000428         2046 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_338047, whole genome shotgun sequence.
ACCESSION   DATCBJ010000428 DATCBJ010000000
VERSION     DATCBJ010000428.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2046)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2046)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2046
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_338047"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1058
                     /locus_tag="VII50_08640"
     CDS             <1..1058
                     /locus_tag="VII50_08640"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HEY5050955.1"
                     /translation="AKISAETDADVEVVRRQARVAVEAVFETAERLLDDSDGDVIWTA
                     SEDRRSVVLRVAPLHVAQLLRTALFDQRTVVLTSATLTLGGRFEPVADGLGLGIPSAP
                     STDDDGRPDWIGLDAGSPFDHGRQGILYVARHLPPPGRDGTPAETLTELAELIEAAGG
                     RTLGLFSSMRAAVDAAEAMRVLVDVPILCQGDDSTAELVRQFAAEPTSCLFGTLSLWQ
                     GVDVPGASCQLVVVDRIPFPRPDDPLMSARSKAVADAGGNGFMAVAAAQAALLLAQGT
                     GRLLRSRTDRGVIAVLDSRLATARYAGFLRASLPPFWFTTDPAVVRSSLRALDEAAGG
                     RRKPVVHEQPTLDDATL"
     gene            1095..1532
                     /locus_tag="VII50_08645"
     CDS             1095..1532
                     /locus_tag="VII50_08645"
                     /inference="COORDINATES: protein motif:HMM:NF037170.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY5050956.1"
                     /translation="MAYYFQVTVDSADPHVLADWWAETLGWEVEPSDEAFIRDMIAKG
                     YATDADTKQHKGVLVWREGSAIRDPDEPASPSRKRILFQLVPEPKTVKNRVHLDVRVG
                     AANVENVLEGVQARGGTFLHRASQGPHWWVTVADPEGNEFCIT"
     gene            1668..>2046
                     /locus_tag="VII50_08650"
     CDS             1668..>2046
                     /locus_tag="VII50_08650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5050957.1"
                     /translation="MGGVIPAGFEAYARILHPADSADEHARWSAVAAACGKVAHALMQ
                     FHTLVGVGPTEYDVDSAAWTGSVPDQGDLEPESLAALLDVLARHTGLVQQCWFGVWEG
                     YGWLRGGGPILVATQDGALPPPSA"
BASE COUNT          302 a          642 c          721 g          381 t
ORIGIN      
        1 gcgcgaagat ctcggccgag acggatgccg acgtcgaggt cgtgcgacgc caggcgaggg
       61 tcgcggtgga ggcagtgttc gagaccgccg agcggctgct cgacgactcc gacggcgacg
      121 tgatctggac ggcgtctgag gatcgtcgta gcgttgtgtt gcgcgttgca ccgctgcacg
      181 ttgcgcagtt gttgcgcacg gcgctgttcg atcagcgcac ggtcgtgttg acgtcggcga
      241 cgttgacatt gggcggacgt ttcgagccgg tcgccgacgg gctcgggctc ggcatcccga
      301 gtgctccaag caccgacgac gatggccggc cggattggat cggcctcgat gccggatcgc
      361 cgttcgacca cggccggcag ggaattctct atgtggcaag gcatcttccg cctcctgggc
      421 gcgatggcac gccagcggaa acgctcaccg aactagccga gctcatcgag gccgcgggtg
      481 gtcgcacgct cgggctcttc tcatcgatgc gcgccgcagt cgatgcggcc gaagcgatgc
      541 gggtactggt cgacgtcccc attctatgtc agggtgacga ttcgaccgca gaacttgtgc
      601 gtcagtttgc ggctgagccg acgtcgtgtc ttttcggcac gctgtcgttg tggcagggcg
      661 tcgacgtccc gggggcgtcg tgtcagctgg tggtcgtcga ccgcatcccg ttcccgcggc
      721 ccgatgatcc gttgatgtcg gcgcggtcca aggccgtggc cgacgccggt ggcaacgggt
      781 tcatggcggt cgcggccgcg caggcggcgt tgttgctggc acaaggcacc ggacggttgt
      841 tgcgctcgcg caccgatcgt ggcgtcatcg cggtgctcga ctcgcggttg gcgaccgccc
      901 gctacgcggg attcctgcgg gcgtcgctgc cgccgttctg gttcacgacg gatcccgccg
      961 tcgtccgctc gtcgttgcgc gctctcgacg aagctgcagg cggacgccgg aagcccgtgg
     1021 ttcatgaaca gccgacgctc gacgacgcga ccctctgacg tcttgttgtc ggcgtcgggt
     1081 cgtaggttct gcgcatggcc tactacttcc aggtgaccgt cgactccgct gacccgcatg
     1141 tgctcgccga ttggtgggcc gaaacgctcg gctgggaggt cgagcctagc gacgaggctt
     1201 tcattcgcga catgatcgcc aaggggtacg cgaccgacgc agataccaag cagcacaagg
     1261 gtgttctggt ctggcgtgag ggttcggcga tccgcgatcc cgacgagccg gcgtccccgt
     1321 cacgcaagcg catcttgttc cagctcgtgc ccgagccgaa aacggtcaag aaccgggttc
     1381 acctcgacgt tcgggtggga gcggccaacg tcgagaacgt tctcgagggc gtgcaggcgc
     1441 ggggcggcac cttcttgcat cgcgcgagtc agggcccgca ctggtgggtg accgtcgccg
     1501 acccggaggg caacgagttc tgtattacct agacgggtgg cggtacctcc ggggaagcca
     1561 gtaatcgatg acacactgcc cgggtgccat ccgacggcct cacacaaacc gacgacttga
     1621 ccgccgcagc gtggattgct ccgaggctgg gcgcatttgc gtctggcgtg ggtggcgtta
     1681 ttccggccgg cttcgaggct tacgcgcgca ttctgcatcc ggccgactcc gctgacgaac
     1741 atgcgcgctg gagcgcggtt gccgcggcgt gcggcaaggt cgcgcacgca ttgatgcagt
     1801 ttcacaccct cgttggagtt ggaccgaccg agtacgacgt ggattccgcc gcatggacgg
     1861 gctcggtccc cgaccaagga gatctcgaac ccgagtcgct tgcggcactg ctcgacgtcc
     1921 tcgcgcgaca cacgggtttg gtgcagcaat gctggttcgg ggtctgggag ggatacggct
     1981 ggctcagggg cggcggccca atcttggttg cgacccagga tggcgcacta ccgcccccat
     2041 cggctg
//
LOCUS       DATCBJ010000429         5100 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_266885, whole genome shotgun sequence.
ACCESSION   DATCBJ010000429 DATCBJ010000000
VERSION     DATCBJ010000429.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 5100)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5100)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5100
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_266885"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(21..629)
                     /locus_tag="VII50_08655"
     CDS             complement(21..629)
                     /locus_tag="VII50_08655"
                     /inference="COORDINATES: protein motif:HMM:NF015325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEY5050958.1"
                     /translation="MATLRALVLGCSLKTSPASSSSELLGMQVLTALSDHDVDGEFVR
                     VVDHGIKFGVSTDEGPDDGWPAIRTKLLAADVLILATPIWMGQPSSVCKMVLERLDAE
                     LSEKDQRGRLLTYGKVAAVAVVGNEDGAHHVTAEVLQSLNDVGFTIAAAASTYWVGEA
                     MHKVDYIDAGPKPDTTGAATKTLAANAAHLARLLLADPYPPS"
     gene            2227..2529
                     /locus_tag="VII50_08660"
     CDS             2227..2529
                     /locus_tag="VII50_08660"
                     /inference="COORDINATES: protein motif:HMM:NF037625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5050959.1"
                     /translation="MITTSPALLARERQVFAEHTAELAAVLAEQTDAHPEDLAPWVVA
                     NALMGVHRAILDNVRRRALADQPNPSLARDVRLETERALALLETGLGGYPDDHARR"
     gene            2510..2656
                     /locus_tag="VII50_08665"
     CDS             2510..2656
                     /locus_tag="VII50_08665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5050960.1"
                     /translation="MTTHADSDGALAAGRADAHTIVDSVIDAALAHCGGELADAAALA
                     ILAT"
     gene            complement(2967..3089)
                     /locus_tag="VII50_08670"
     CDS             complement(2967..3089)
                     /locus_tag="VII50_08670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5050961.1"
                     /translation="MPLQTVWRCLRDTYVRVPRATEGESVDLDAELRYLLAHPN"
     gene            complement(3547..4839)
                     /locus_tag="VII50_08675"
     CDS             complement(3547..4839)
                     /locus_tag="VII50_08675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY5050962.1"
                     /translation="MVTSDTFVIVGAGLAGAKAAEALRSQGFDGRLILLGGEHHAPYE
                     RPPLSKDYLMGKSPREKVFAHAHSWYAEHQVDLRSAQFVTGLDRGARGVALADGSVVS
                     YDKLLLTTGASPRELRVSGADASGVQYLRTIDDSDALRATLATASRLVVIGAGWIGLE
                     VAAAARLAGVEVTVVETAELPLLRVLGPEVSQVFADLHQEHGVKFHFNALLAEILTDD
                     GVTTGVQLKDGTTIAADAMLVAVGAVPNVQLAEAAGLRVDNGIVVDAALRTSDPDIFA
                     AGDVANAFHPLFDKHIRVEHWANARQQPKAAAASMLGHDASYDELPYFYTDQYDLGME
                     YVGYVEPGGYDRVVFRGDVAGREFVAFWLSNDRVVAGMNVNIWDVVEPIKTIIRSRAA
                     IPASRLADVGDALSDLAIEATSQSSLGAAPVRANGQQP"
     gene            complement(4868..>5100)
                     /locus_tag="VII50_08680"
     CDS             complement(4868..>5100)
                     /locus_tag="VII50_08680"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020657899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GTP cyclohydrolase I"
                     /protein_id="HEY5050963.1"
                     /translation="FARGLQVQERLTKQLADWLDVNLHPKGVGVVIEAEHQCMTLRGV
                     RAHESRTITSALQGHLRHDAAARLEFFALTLR"
BASE COUNT          924 a         1712 c         1594 g          870 t
ORIGIN      
        1 cccagccccg ccaccccggc tcacgacggc gggtaggggt cggcgagcag cagccgcgcc
       61 aggtgcgcgg cgttggccgc caatgtcttg gttgccgcgc cggtggtgtc tggtttgggt
      121 ccggcgtcga tgtagtcgac cttgtgcatt gcctcaccca cccagtaggt agaggcggcc
      181 gccgcgatgg tgaaaccgac atcgttcaac gattggagga cctccgcggt cacgtggtgc
      241 gcgccgtcct cgtttcccac gaccgccacc gcggcgacct tgccgtaggt caacagccgg
      301 ccacgctggt ccttttccga cagctcagcg tcgagccgct cgaggaccat cttgcacaca
      361 ctggatggct gtcccatcca gatcggtgtc gcgaggatga gaacgtcagc ggccagcagc
      421 ttggtacgga tggccggcca accgtcgtcg ggcccttcgt cggtggatac accgaacttg
      481 atgccgtggt cgacgacccg gacgaattcg ccgtccacgt cgtgatcgga caaggcggtg
      541 aggacttgca tgcccaggag ctccgaactc gacgacgctg gcgatgtctt cagtgaacag
      601 ccgagcacca gcgcgcgcag tgtcgccatc gtttcgttct cccctggtcc tggagcggtg
      661 gtggccttcc ttcccacggc tgtacccccg gggcggcggc gcgaagccga ttcggcgacc
      721 tcctgcagtc ccgaactcct gtggtgccga atatgtcgtc cgggataaat cgctgatacc
      781 aaccgttcat gcgtagtacc acacgcgcga ccacaaccgc acatccaagt cgcgccacaa
      841 caagatcgcg cgccgtccca gcaacccgcg gttgcgcgtc agcccggtcg caacgaccga
      901 ggaccacaaa cgcgcaaggc ggggccgtag cggggccgta cggccggtaa acttggggcc
      961 caatacggac caccagcggt caagatcaaa aacaggtatg cgcaggtcag cgcggatatt
     1021 cacgctcaac cgggaaggtg tcccgctagc cttccgagtt ggcaatgccg gttcgatcct
     1081 tgtcgcccgc tcccccgaaa acccttgcaa aataagggtt ttcgcgcgct caagaggcca
     1141 cgggcttgaa taacggtacg acgtcgtcgt ttgagctgtc cggggtgacc gtgcagcgcg
     1201 tcgagggcgc atctcacgga tcgcggaggc caaggccgcg cgtaaggctc gcgtccactt
     1261 gctcgtcaaa ccgtaggcca tcgaacgact cggcccaccc gaacaagagg ttgttcctcg
     1321 gagccggatc aagatcatcc gtgcccccgg tggttgcacc aatccgtggt aacggggcgc
     1381 aacggtcagc atccgggcgc tcgttttccc ttacaccgca ggcaaacccg cgatttcgac
     1441 ccgattcacg ctcaaccaac accggcggtg cagatggttc aagtcccccc tcggacacgg
     1501 tgtttgtgca ggtcacagac ctacgccgct gcttttggtg cacccatggt tgccacccca
     1561 gacgtccaag cggacagccc gcgttcccta ccaggtgtat caacatgttg gtcccccttg
     1621 gtccccctct ggtcgttgcg cgttggcgtc agctcgatct cgaatcgcaa ggatgacgcg
     1681 ttcatcaaca gatcccgaca tcgctggact cgcgcaacct gctagccggg cagccggggc
     1741 cggtgagcgc gaacaccgca gctccgggca gcggcgtgtg ttgcatcgaa agcaggttga
     1801 cttgcccgtt cgatcccggg cacccgctcc actgaaagcc cttacatagc aagggttttc
     1861 aaccgcccac ggactccgcg tgcttccgaa acgtagatta tgggtacctc gcggggccgc
     1921 gtgtacgacg ccgccacggc agggccccag cccggcaagc tggccctgcc tgttctccgg
     1981 tcacccacgc gctccaccga aaacccgcgc atgatggtga gcgaaacgcc ggccagcgcc
     2041 ggcggggtgc gactcacagt ccccagacgc cttgccgtac caggccgaca ttcaagaagc
     2101 actgttgcct cccttatatg tcaatgaggc gtggtcacag cctgtcgatg atgcgccggg
     2161 aacggaccga agaacggtcc cgtcggcccg ggttctctgg cgtagcgtca ctgcctcacg
     2221 cgtcgcatga tcaccaccag cccggcatta cttgcccgag agcggcaggt tttcgccgag
     2281 cacacggcag aactcgccgc cgtccttgcc gagcagaccg atgctcaccc cgaagacctc
     2341 gcgccctggg tggtcgccaa cgcactgatg ggtgtccacc gcgccattct cgacaacgtc
     2401 cggcgcagag cattggcaga ccaacccaac ccaagcctcg ctcgtgacgt gcgcctcgag
     2461 accgagcgcg ccctcgcact gctcgaaaca ggactcggcg gctatcccga tgaccacgca
     2521 cgcagatagt gacggcgcct tagccgccgg ccgcgccgac gcccacacca tcgtcgactc
     2581 agtcatcgac gccgcgttgg cccactgcgg cggcgaactc gctgacgccg ccgccctagc
     2641 catcctcgcc acatgaccgg caacaattgt ttggttgcaa ccatgtgcct cgaggaggtt
     2701 aaccggctac gcgggcacgt ccgttaggtg aaagtaccag cagtcccaga ttgacgggac
     2761 tgccccagct ttcgttgact tgacctgccc tacggattgg ttacgcgttg agtgctgagg
     2821 ctcacgcggg gagttgcgat cgttgtgccg ctaccacgaa ctggttcgca gcgagaggta
     2881 tccgagctgg gagtgccggc gttggcgatt gtcgaagacc tcgatgccgt ggccaagcac
     2941 ctctaggagg ccgtcgtagt ggaccgttag ttcggatgtg ccagcaggta ccgcagttcg
     3001 gcatcgaggt caaccgactc accttccgtc gcgcgaggga cgcggacgta ggtgtcacgc
     3061 aggcaccgcc acacggtctg taggggcacg acccactccc ctgccgttgg atcggctcgc
     3121 cagcgcagat gcaggagttg agggggtacc cggccaagcg gggtaaccca cagctccagg
     3181 tcggccgagc gtgcccggcc ccgcagtcct cgcgccagcg tcgaccgcaa caaagctgcg
     3241 cggacgtggg tctgtcccgt acggagtacg gcgtcgatga ccgatggagt ccgaaggtcg
     3301 aaggctagat cgatgcgcgc ttcacatcca gacgcacggc ccagcaccgc gacgtgaacg
     3361 cttgatccag acgagctgat catcgactcc tccggcacgg ggaccctgcc gatgccaatc
     3421 aaccaccacc acgatatcgc atcgcgtcgc acccagatcg gttagcgcga ctcggacgag
     3481 ctgcgttgct cgcggaaaag ttgctcgcgg aaacgcagcg tggccacgct cggccggacc
     3541 gacttctcac ggctgttgtc cattcgcccg gaccggcgcc gctccgagcg acgattgact
     3601 cgtcgcctcg atagcgagat cggagagagc gtcgccgacg tcggctaggc ggctcgccgg
     3661 tatcgcggcg cgggatcgga tgattgtctt gatcggttcg acgacgtccc agatgttgac
     3721 gttcatccct gcgacaacgc ggtcgttcga gagccagaaa gcgacgaact cccggccagc
     3781 gacgtcgccg cggaagacga cccggtcgta gccgccgggc tcgacgtagc cgacgtattc
     3841 catcccgagg tcgtactggt cggtatagaa gtacggcagc tcgtcgtacg aggcgtcatg
     3901 tccgagcatg gatgcggctg ccgctttcgg ttgctgtcgc gcgttggccc aatgttcgac
     3961 ccggatgtgc ttgtcgaaaa gcgggtggaa cgcgttggcc acatcacctg cggcgaagat
     4021 gtcggggtcg ctggtccgta aggcggcgtc gacgacaatg ccgttgtcaa cgcgcagccc
     4081 cgcagcctcg gcaagttgca cgttggggac ggcacccacc gccacgagca ttgcgtctgc
     4141 cgcgattgtc gtgccgtcct tcagctggac gccggtggtc accccgtcgt cggtgaggat
     4201 ctcggcaagt aaggcattga agtggaactt gaccccatgt tcctggtgca ggtcggcgaa
     4261 tacctgtgac acctcgggtc cgagtacgcg cagcaggggc agttcagcag tttcgacgac
     4321 ggtcacctcg acgccggcca ggcgggccgc ggcggcgact tcgaggccga tccagccggc
     4381 gccgatcacc accaatcgcg acgcggtggc aagggtcgcg cgtagcgcat cggagtcgtc
     4441 gatcgtgcgc aggtactgca cgccgctagc gtccgccccg gagacccgca gctcacgcgg
     4501 gctcgccccc gtggtcaaca gaagcttgtc gtaactgaca accgacccat cggcgagcgc
     4561 gacgccccgc gcgccacggt caaggccggt aacgaactgc gcagacctca agtcgacctg
     4621 atgctcggcg taccaggagt gggcatgggc gaagaccttt tcgcgcggac tcttgcccat
     4681 caggtagtcc ttcgacaacg gcggccgctc gtacggcgcg tggtgctcgc cgcccagcag
     4741 gatcagccgg ccgtcaaaac cctggctacg caaggcttcc gcagccttgg cgccggcaag
     4801 cccagcgccg acgatcacga atgtgtcgga cgtaaccacg gagcggccct tctgatcgag
     4861 agagcgatta gcggagggtc agtgcgaaga actcgaggcg agcagcggcg tcgtggcgca
     4921 ggtgcccttg cagcgctgag gtaatggtgc gcgattcgtg ggctcgcaca ccacgcaacg
     4981 tcatgcattg atgttccgcc tcgatcacca ccccgacgcc cttggggtgc aggttgacgt
     5041 cgagccagtc ggccagctgc ttggtcagcc gctcctggac ctgcagtccc cgcgcaaacc
//
LOCUS       DATCBJ010000430         3758 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_413531, whole genome shotgun sequence.
ACCESSION   DATCBJ010000430 DATCBJ010000000
VERSION     DATCBJ010000430.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 3758)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3758)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3758
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_413531"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1019)
                     /locus_tag="VII50_08685"
     CDS             complement(<1..1019)
                     /locus_tag="VII50_08685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetoin utilization protein AcuC"
                     /protein_id="HEY5050964.1"
                     /translation="MHPLTVVWSDELVAYDFGPHHPLDPVRVVLTIELARALGVLDAP
                     NVTLVPPDPCNDSMLELVHTKDFLDAVKAEVGNAHFGLGTPDVPVFEGMHEASARVVG
                     ATVAAARAVYEGRALHAVNVAGGLHHAMPAAASGFCVYNDPAVAIAWLLEQGLQRVAY
                     VDIDVHHGDGVEAAFYDDPRVLTISVHQSGRTLFPGTGFPHEAGGPRADGHAVNVALP
                     AGTNDAGWLRAFHAVVPPLLRSFEPEILVSQHGCDSHRLDPLAELDLTVDGQRAAHAA
                     LHDLAHELTGGRWVAVGGGGYALAQVVPRTWTHLLAIAAGAPIDPATPTPQSWRADAR
                     ARTGGP"
     gene            complement(1050..1859)
                     /gene="proC"
                     /locus_tag="VII50_08690"
     CDS             complement(1050..1859)
                     /gene="proC"
                     /locus_tag="VII50_08690"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HEY5050965.1"
                     /translation="MSEQTMIAVIGAGKMGEALVSGLLKAGASPDDLIITERYEERAS
                     ELSQRYGVKSVSNADAAHQADTIVLAVKPQDMDTLLAELAPVVTPDRLIVTIAAGITT
                     SAIERRLAAGVPVVRVMPNTPALVGEAMSAVAPGAHASAEHLDRAEALFRPVGRVVRL
                     PESQLDAVTALSGSGPAYVFYLVEAMTDAGILLGLPRAVAHELIVQTVYGAAVMLRET
                     GEHPVALREAVTSPAGTTISAIRELENHGVRAAFLAALEAARDRSRELAGE"
     gene            complement(1856..2833)
                     /locus_tag="VII50_08695"
     CDS             complement(1856..2833)
                     /locus_tag="VII50_08695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009075674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline dehydrogenase family protein"
                     /protein_id="HEY5050966.1"
                     /translation="MTSISAVATRNSEDDDVLRRLMLSAAASPRVKRGIEVVPLTRHV
                     VDRFVAGDDVGDALAASRKLVAAGLLASIDRLGEATTEPTQAEATASAYVWLLKELAD
                     AGLADFCEVSLKLSSLGQALEKDGERIALEHARTVCAAARDAGTTVTVDMEDHTTTDA
                     TLSAVRELRTDFPSVAAVLQAALLRTEADCADLAGVGSRVRLCKGAYNEPASVAHQDR
                     AAVDLSYVRCLRILMRGKGYPMVATHDPRLIAVSGVMAVQAGRSKGAYEYQMLFGVRP
                     DEQGRLASRGEKIRVYLPYGTQSYPYLMRRLAERPANLAFFLRAVTSRG"
     gene            complement(2866..3603)
                     /locus_tag="VII50_08700"
     CDS             complement(2866..3603)
                     /locus_tag="VII50_08700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009082703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY5050967.1"
                     /translation="MNRAITFATARRVLNQLRHDPRTIALLLVVPALLLSLLRWLLTN
                     ERANFDHIGAPVLAIFPFVVMFIVTSVATLRERTTGTLERLLTMPMNKLDLLLGYSLA
                     FGAVAVVQVGLCAALALGPLGLHIAGAGWLLFVVGVLDALLGMALGLFLSAFARTEFQ
                     AVQFMPAVVFPQLLLCGLLTPRSSMDAALRAISDVLPLTYAIDAMQRVSESSTFNHQI
                     GVDLLVVSATAVAALILGAITLRRRTA"
BASE COUNT          714 a         1260 c         1211 g          573 t
ORIGIN      
        1 gggccaccgg tacgggcgcg cgcatcagcc cgccatgact gcggcgttgg ggtggccgga
       61 tcgatgggcg cacccgcggc gatcgcgagg agatgagtcc aggtgcgggg aaccacctgc
      121 gccagcgcat aacccccacc accgacagcc acccatctgc cgccggtgag ctcgtgtgca
      181 aggtcatgca gggccgcgtg cgcggcccgt tgcccgtcaa cggtcagatc aagctcagcg
      241 agcgggtcga gtcgatggct gtcgcagccg tgctggctga ccagaatctc gggctcgaag
      301 ctacgcaata agggcgggac gacggcgtgg aacgcccgca gccagccggc gtcgttcgtt
      361 ccggccggca aggccacgtt cacggcgtgg ccgtccgcac gcggaccacc cgcctcatgg
      421 ggaaagccag taccgggaaa gagcgtccgt ccgctttgat gcaccgagat cgtgagcacc
      481 cgcggatcgt cgtagaaggc ggcctctaca ccgtcgccgt ggtgaacatc gatgtcgacg
      541 taggcgaccc gctgcagacc ctgctccagc agccaagcga tcgccaccgc gggatcgttg
      601 tacacgcaaa acccagacgc agcagccggc atcgcgtgat gcaaaccacc tgccacgttg
      661 acggcgtgca gcgcacgacc ttcgtagaca gcccgagcgg cagccacagt ggcgccgaca
      721 acacgggctg atgcctcgtg cataccttcg aagaccggaa cgtcgggcgt accgagtccg
      781 aagtgcgcgt taccgacctc agccttcact gcgtcgagaa agtccttggt atgcacgagt
      841 tcgagcatcg agtcgttgca cggatccggc gggaccaggg tcacgttcgg tgcatcgagg
      901 acgccgagtg cgcgcgcaag ctcgatggtc agcacaacac gcaccgggtc gagtgggtga
      961 tgcggaccga agtcatatgc gacgagttcg tcagaccaga cgacggtcaa gggatgcatg
     1021 ggatccacac tagtgagcgg gcgcgcgcac tactcgccgg cgagctcgcg cgaccggtca
     1081 cgcgcagcct cgagagcagc gagaaacgca gcccgcacgc cgtggttctc gagctcgcgg
     1141 attgcggaga tggtagtgcc cgcaggtgag gtgaccgcct ctcgcaacgc caccgggtgc
     1201 tcaccggttt cgcgcagcat cacggcagcg ccgtacacgg tctgcacgat cagctcatgc
     1261 gcgacggcgc ggggcagccc cagcagaatg cctgcatcgg tcattgcttc gaccaggtag
     1321 aagacgtacg ccgggccgct gcccgagagt gcggtaaccg cgtcgagctg cgactccggc
     1381 agtcgcacga cccggcccac cggccgaaag agcgcctccg cccggtcaag gtgttccgcc
     1441 gacgcgtgag cgcctggggc caccgcgctc atggcctcgc ccaccagcgc cggagtgttg
     1501 ggcatgacac gtacgacggg gacgccggcg gccagccggc gttcgatggc ggacgtcgtg
     1561 atgccagccg caatcgtcac gatcaggcgg tccggcgtaa ccaccggcgc aagctccgcc
     1621 aggagcgtgt ccatgtcctg cggcttgacg gcgagcacga tcgtgtcggc ttgatgggca
     1681 gcgtcagcgt tgctcaccga cttcacgccg tagcgttgag agagctcgga cgcccgttct
     1741 tcatagcgtt cggtgatgat gaggtcatcg gggctagcgc ccgccttgag cagaccggag
     1801 accaacgcct cccccatctt gcccgcaccg atcaccgcga tcatggtctg ctcactcatc
     1861 ctctgcttgt taccgctcga aggaagaacg caagattggc gggccgttcg gccagcctgc
     1921 gcatgaggta cggatatgac tgggtgccgt agggcaggta cacgcgaatc ttctcaccac
     1981 gggaagccag ccgaccctgt tcatcgggcc tgacgccgaa caacatctga tattcgtatg
     2041 cgcccttcga gcgccccgcc tggacagcca tcacgccact caccgctatc aagcgcgggt
     2101 cgtgggtggc caccatcgga taacccttgc cccgcatgag aatccgcagg caccgcacgt
     2161 aggacaggtc gacagcggcc cgatcctggt gggcgaccga cgccggttcg ttatacgcgc
     2221 ccttgcacag ccgtacccgg ctaccaacgc ctgcgaggtc ggcgcagtcg gcctccgtcc
     2281 tgagcaaggc cgcctggagc actgcagcga cactgggaaa gtcggtgcgc agttcccgca
     2341 ccgccgacag cgtcgcgtcg gtggtggtgt ggtcttccat gtccaccgtc accgtggtgc
     2401 cagcatcgcg cgccgcggca cacacggttc gagcgtgctc gagggcgatc cgctcaccgt
     2461 ctttctccag tgcctgcccg agcgacgaca gcttgagcga aacctcgcag aagtccgcaa
     2521 gcccggcgtc cgccagttcc ttcagcaacc acacatatgc ggacgcggtc gcctccgcct
     2581 gcgtcggctc ggtcgtcgcc tcgcccagcc ggtcgatact ggcgagcagc ccggccgcga
     2641 ccagcttgcg gctcgcggcc agcgcgtcac cgacgtcatc gccagcaacg aaacggtcaa
     2701 ccacgtgcct ggtgagaggg acgacctcga tccctcgctt gacgcgtggg ctcgcggcgg
     2761 ccgacagcat caggcggcgc agcacgtcgt cgtcctccga gtttctcgtc gctaccgccg
     2821 atatcgatgt catcggggcg cgaatccagg ctacagcggg ctggctcagg cagttcgacg
     2881 gcgcagcgtg atcgcgccca ggatcaaagc tgctactgcc gtcgcactca cgacgagaag
     2941 gtccacaccg atctggtggt tgaacgtgct cgactcgctc acccgctgca tggcgtcgat
     3001 cgcgtaggtc agcggaagca cgtcggagat cgcgcgcaac gcggcgtcca tcgaagaacg
     3061 tggcgtgagg agtccgcaga gcagcagttg agggaatacc accgccggca tgaactggac
     3121 cgcctgaaac tcggtgcggg caaacgccga caggaagagg ccaagtgcca tgcccaacaa
     3181 cgcgtcgagc acaccgacga cgaacaacag ccatcccgca ccagcaatgt gcaagccaag
     3241 ggggcccagc gcaagcgccg cgcagaggcc gacttgcacc accgcgacag caccaaaagc
     3301 gagcgagtac ccgagcagca gatcgagctt gttcatcggc atcgtgagca agcgctcgag
     3361 agttccggtc gtgcgttcgc gcagcgttgc gaccgacgtc acgatgaaca tgacgacgaa
     3421 cggaaagatc gccaacaccg gcgcgccgat gtgatcgaag ttcgcgcgct cgttggttag
     3481 cagccaacgc aacaacgaca acagcaatgc cgggacgacg aggagcaacg cgatcgttcg
     3541 cgggtcgtgt cgcagttggt tgaggacacg ccgggcggtc gcgaaggtga ttgccctgtt
     3601 catgtcgcct cgatcaatcg caggaatacc tcgtccatgt cctccacgcc tgtgtgccgg
     3661 cgcaattcgt cgggtgcacc gcgcgccaga attgcgccgt cgcggagcag cagcaactgc
     3721 tggcaatggg tcgcttcttc catcacgtga ctcgagat
//
LOCUS       DATCBJ010000431         2902 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_113824, whole genome shotgun sequence.
ACCESSION   DATCBJ010000431 DATCBJ010000000
VERSION     DATCBJ010000431.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2902)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2902)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2902
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_113824"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(80..460)
                     /locus_tag="VII50_08705"
     CDS             complement(80..460)
                     /locus_tag="VII50_08705"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009085597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HEY5050968.1"
                     /translation="MATNEKPAVEVPTDQPPSYQLELEDVIVGSGEEAVSGRVVEVHY
                     VGVSWSTGQQFDASWDRRDTFKFKLGKGQVIAGWDQGVAGMKVGGRRRITIPPMLAYG
                     KRGAGGVIGPDETLVFVVDLIAVN"
     gene            514..843
                     /locus_tag="VII50_08710"
     CDS             514..843
                     /locus_tag="VII50_08710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5050969.1"
                     /translation="MDPDRARQLLERERERIERAIGDLDPGPAGDRDGPGEDGSEDLY
                     QKEFDEGRAGNLREQLDALERAEARLAAGTYGLSVESGKPIPEERLEFLPTAERTVEE
                     EQANGGG"
     gene            complement(880..1425)
                     /locus_tag="VII50_08715"
     CDS             complement(880..1425)
                     /locus_tag="VII50_08715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5050970.1"
                     /translation="MRVAIADHFGWAVAVTASADHEVVDRRRIELVEPGVSAAPIHYE
                     SRRLDVAATAALVANTRASVARTASAALDELATALPAPVLSISLRVWPLDFPDEIAVQ
                     RRAPYESRADAIMYRQELAELAHVRSWEVHLYDAKAVVDQAARMLAEPVDEVLQRPRA
                     TIGPPWTKDHRIALAATIVAG"
     gene            1477..2763
                     /gene="otnK"
                     /locus_tag="VII50_08720"
     CDS             1477..2763
                     /gene="otnK"
                     /locus_tag="VII50_08720"
                     /EC_number="2.7.1.217"
                     /inference="COORDINATES: protein motif:HMM:NF043035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxo-tetronate kinase"
                     /protein_id="HEY5050971.1"
                     /translation="MTWPSRKAQRVVGCIADDLTGATDLGATLAARGMRTVILIGSDV
                     PDQLADGAEAIVVALKSRTTPVAHAVEESLAALRLLQRLGFSRFIFKYCSTFDSTAQG
                     NIGPVAESLLDALGQDLTVVCPAFPANGRTVDGGTLFVWGIPLDRTTMATHPLTPMRD
                     SSVMRLLAAQVDGSIALMPLHEVRRGPDAVREQLASMAEQGIRYVVPDAIDDADLVTI
                     GEGAHDLSLVTGSSAVALGFPSDFVPSGGQPLSGQGIKPDGDSLIISGSCSQATQRQC
                     ASFSGLHAARAFNVAAHLGGDEQISSTVEWVCGQRLGEPALVYSTAPPDEVADIQASF
                     DRTEVGRTVEEALAEIAAGVVNRGRVRALVVAGGETAGAVVRRLGVRALAVGATIEPG
                     VPWTFTLGQEPLALALKSGNFGSDHFFSTAFEALRN"
BASE COUNT          480 a          944 c          972 g          506 t
ORIGIN      
        1 acgcacatcg cgcccggcgt gccggtgacg atcaccggct gagcgtcgcc cgcgcggccc
       61 gactgcgccc cggcctcggt cagttgacgg cgatcaggtc gaccacgaac acgagcgtct
      121 cgtcggggcc gatgacgccg ccggcgccgc gttttccata tgccagcatc ggcgggatcg
      181 tgatccgccg ccgtccgccg accttcatgc cggccacgcc ctggtcccag ccggcgatga
      241 cctgtccctt gccgagtttg aacttgaatg tgtcgcggcg gtcccaggac gcgtcgaact
      301 gctggccagt gctccacgag acaccgacgt agtggacctc cacgacgcga ccgctcacgg
      361 cctcctcgcc gctcccgacg atgacgtctt caagttcgag ctggtaggac ggcggctggt
      421 ccgtcgggac ctcgaccgcg ggcttctcgt tggtggccac aacggcaccg taccgatgtg
      481 tgcccgcgca ccgctcgcgg tactgttccc cccgtggatc ccgatcgcgc ccgccaactg
      541 ctcgagcgcg agcgggaacg aatcgagagg gcgatcggcg atctcgaccc cggacccgcc
      601 ggcgaccgcg acgggccggg cgaggacggc tccgaggacc tctaccagaa ggagttcgac
      661 gagggccgcg ccgggaacct gcgcgagcaa ctcgatgcac tcgagcgcgc cgaggctcgg
      721 ctcgccgccg gaacctatgg gctgtcggtc gagagcggga agccgattcc cgaggagcgg
      781 ctggaatttc tcccgaccgc ggagcgcacc gtcgaggaag agcaagccaa cggcggtggg
      841 taaccgctcg cgggcgcgtg caccgcggtc ggcccagaat cagccggcca caatcgtcgc
      901 cgcgagcgcg atccgatggt ccttcgtcca aggtggaccg atcgttgccc gcggtctctg
      961 caggacctcg tcgaccggct cggccagcat cctggccgct tgatctacaa ctgccttcgc
     1021 gtcatagaga tggacctccc agctacgaac gtgtgcgagc tcggcaagct cctggcgata
     1081 catgatggcg tcggcccggg actcgtaggg cgcacgacgt tgcacggcga tctcgtcggg
     1141 gaagtcgagc ggccagactc ggagcgagat cgagaggact ggtgcaggca gcgccgttgc
     1201 gagttcgtcg agtgccgctg aggccgtccg agcaacggac gctcgcgtat tcgcgaccag
     1261 cgcggccgtc gccgcgacat ccagacgccg gctctcgtag tgaatcggtg ctgccgacac
     1321 gccgggttcg accagctcga tccgtcggcg gtccactacc tcgtgatccg ccgacgccgt
     1381 caccgcaacc gcccagccga aatgatccgc aatcgcgacg cgcatgccgc caattgatac
     1441 aggcatcgac gtcgaccagt cgatacggtc gctcaaatga cctggccgtc cagaaaggcg
     1501 cagcgcgtcg ttggctgcat cgctgacgac ctgaccgggg cgaccgatct cggcgcgacg
     1561 ctcgccgctc gcggcatgcg cactgtgatt ctgatcggaa gcgacgtgcc cgatcaactc
     1621 gctgacggcg cggaggcgat tgttgtcgcg ctcaagtcgc ggaccacgcc cgtggcccat
     1681 gcggtagagg aatcgcttgc ggcacttcgc ttgctgcagc ggcttggatt ctcccggttc
     1741 atcttcaagt actgctcgac attcgactcg acagcgcagg ggaacattgg acccgtcgca
     1801 gagagcctgc ttgacgcgct cggccaagac ctgacagtcg tatgtccggc cttccccgcg
     1861 aacggtcgaa cggtggacgg aggaacgctg tttgtctggg gcataccgct agacaggacg
     1921 acgatggcga cgcatccact gacgccgatg cgcgactcaa gcgtgatgcg tctgctcgcc
     1981 gctcaggtgg atgggtcgat cgccttgatg ccgctccacg aggtgcggcg cggacccgac
     2041 gccgtccgcg agcaattagc gtcgatggcc gagcagggaa tccgctacgt cgtccccgat
     2101 gcgatcgacg acgcggattt ggtgactatt ggcgaagggg cccacgatct gtcgctcgtg
     2161 acgggctcgt ctgctgtagc gctgggcttt ccgagtgact tcgtgccaag tggaggtcag
     2221 ccgctctccg gacaaggcat caaaccagac ggcgactccc tgattatctc gggaagttgc
     2281 tcgcaggcca cgcaaaggca gtgcgcgtcg tttagcggac tccatgcggc acgagcgttt
     2341 aacgtggctg cgcacctggg cggggacgag cagatctcct ccacggtgga atgggtatgt
     2401 ggtcagcgtc tcggtgaacc ggctttggtg tactccacgg cgccgccaga cgaagtcgcg
     2461 gacattcagg cgtcgttcga tcgaacggaa gtcggtcgca cggtcgaaga agccttggcc
     2521 gagatcgctg ctggagtggt caaccgtggg cgcgttcgcg cgcttgtggt tgccgggggc
     2581 gagaccgccg gtgcggttgt gcgtcgcctt ggtgttcgcg cactcgcggt cggcgcaacc
     2641 atcgagcccg gggtcccttg gacgtttacg cttggccaag agccgctcgc gctggcgctc
     2701 aagtcgggaa actttggtag cgatcacttc ttctcgaccg cgtttgaggc gctccgtaac
     2761 tgagcacgtc atggaacgcg aactttggac tgagtctggg tccacgacat gatgtgctag
     2821 ttccttcggg gaagcctgga ggtgtcacgt ttattgtgcc tgtcgagcag agatgctccg
     2881 ccgcctccgg gtgtggcgga gg
//
LOCUS       DATCBJ010000432         8338 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_445785, whole genome shotgun sequence.
ACCESSION   DATCBJ010000432 DATCBJ010000000
VERSION     DATCBJ010000432.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 8338)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8338)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8338
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_445785"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..886)
                     /locus_tag="VII50_08725"
     CDS             complement(<1..886)
                     /locus_tag="VII50_08725"
                     /inference="COORDINATES: protein motif:HMM:NF024793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEY5050972.1"
                     /translation="MNPGSGPASTQVVAAPDRAVLLAELTSTVLAGRNLLLTGPIGIG
                     KTWLAKAAIANADAAGRPSKAIFGTTSSHEVPLAALSPFLTGTEAPAENGLLLARARE
                     AIAATLAASGITLLLVDDAHLLDGLSGLVLRQLMDRQGLQVILVTRVAADLPPALRNF
                     ADGDNMLAIEVNPLDEAGTAALLESTLGGQVSARAAQRMYRLSLGSPLVLRELIRVAL
                     ANDSLRQHRGVWQLDGTLPADAQLAELVSLRLSALDDGRREALELIALSEPLDFDTLL
                     RIAGGPAVEWLEQQGLITI"
     gene            1150..2907
                     /locus_tag="VII50_08730"
     CDS             1150..2907
                     /locus_tag="VII50_08730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY5050973.1"
                     /translation="MPSPHYKWVALSNTTLGMLMATINSSIILISLPAIFRGIGINPL
                     SPGNVGYLLWLMMGYMLVTAVLVVSLGRLGDMLGRVRIYNAGFAVFSVASIILALDPM
                     RGGGGALWLVLWRLLQGVGGAMLFANASAIIADAFPVEQRGLAIGINQVAAIAGSFIG
                     LIAGGLLSVVDWRLVFWVSVPVGVFGTVWAYKSLKDNGRRVQARIDWWGNITFAVGLT
                     AVLAAIVYGIQPYGGHTMGWTAPKVVVAFVAGIGLLAAFGWIELHSDSPMFNLRLFRI
                     RAFAFGGFAGLLAAISRGGMQFMLIIWLQGIWLPLHGYKYEATPLWAGIYLLPLTVGF
                     LIAGPVSGYLSDRHGARTLSTLGMVVVAGSFAGLLLVPTNFAYWIFAVLVLLNGIGGG
                     LFSAPNSTAIMNSVPPEQRGGAAGVQATFLNSGMVLSIGIFFSLMIVGLANTLPSAMF
                     KGLTANGVSAAQAHTIANQPPVGSLFASFLGFNPLQTLLGSQSAAHVSNAQWATLTGK
                     HFFPNLIASPFHHGLVIVFAVAIGLSIVGAIFSMLRGRRYIHGDDVEVVHADHEHRGD
                     PALASGAVPGELALQDDRT"
     gene            2904..3356
                     /locus_tag="VII50_08735"
     CDS             2904..3356
                     /locus_tag="VII50_08735"
                     /inference="COORDINATES: protein motif:HMM:NF024854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEY5050974.1"
                     /translation="MTRPTATVSPDAETAARLRLVISRLARAVRQHGAAGLTPSQLSA
                     LATIEELGPMRMSDLAGHELIGAPVATRVVATLQELGLVQRAGGSTDGRVSLIELSDA
                     GRETLAELWSERAAGLTARIERLKPEQVAVLTSALPVLETLVRDRVIV"
     gene            3468..3737
                     /locus_tag="VII50_08740"
     CDS             3468..3737
                     /locus_tag="VII50_08740"
                     /inference="COORDINATES: protein motif:HMM:NF012576.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEY5050975.1"
                     /translation="MAKAKGSDMITNARRLQLVRPSVTLEEVSSAAGVSKSTASRALC
                     NEPNVKAETRARVQAIAAGLGYVPNEAARALAARRQVVMSGTRAG"
     gene            complement(3746..4009)
                     /locus_tag="VII50_08745"
     CDS             complement(3746..4009)
                     /locus_tag="VII50_08745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5050976.1"
                     /translation="MAEGVAVNDWPGVGDAGGGAGRVAAGAPTCALDNGTTDDALNDS
                     DDNPGNPDDARDPTALPACEQPASIPNAARLTPAHRRPRIFLL"
     gene            4020..4916
                     /locus_tag="VII50_08750"
     CDS             4020..4916
                     /locus_tag="VII50_08750"
                     /inference="COORDINATES: protein motif:HMM:NF013073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HEY5050977.1"
                     /translation="MTGVAYVDVSVATLWHSPSSPRAIDAAELRQPAGVVAWLGAMTT
                     AQRLALYGRVDSQLLLGEKVVVDRQQGTWAHVVVPDQPTPLDARGYPGWIPAAQLTSR
                     APAAAANDVVVLAPSAWLYGPDQVTRVAEVSMATRLPALWADTSWVHTQLPDGRAVLL
                     RAADAAVMAHLGAPLPATAADVVRTAKLFLGLQYLWGGTSAFGFDCSGLTHLVFELRG
                     ITIPRDADAQARVGQAVARADLQPGDLVFFASNGLVHHVGIYVGNNMMVSSLQTGSPV
                     EYSSLAAQPFASEYSGARRLVG"
     gene            4929..6191
                     /gene="codA"
                     /locus_tag="VII50_08755"
     CDS             4929..6191
                     /gene="codA"
                     /locus_tag="VII50_08755"
                     /inference="COORDINATES: protein motif:HMM:NF005748.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine deaminase"
                     /protein_id="HEY5050978.1"
                     /translation="MLDLLIRNAQVRGTEGLTDIGVRGGVFAPPEADAPEVLDVAGAL
                     VTPPLVEPHIHLDAVLTVGQPRPNRSGSLFEGIAVWSERVKDLTVDDVKSRVREVLRW
                     QLANGVQHVRSHVDVCDPELTALRALIEIREEARGQVDLQLVAFPQLGIVSFDGGREL
                     MRKAVELGADVVGGIPHYELTREDGVESVKFSFALAEEFGLRVDIHCDETDDDHSRFV
                     EVMAAETIRRCMSGRVTASHTTAMHSYNAAYAYRLVNNIKRAGMHMVTNPLDNAVLQG
                     RFDTGPIRRGHTRVKQLQEAGVNVCIGHDSVMDPWYPLGYGDPVQAAFVFVHLGHYSG
                     DDELRRLLDMVTVNPAAALGIEGYGIVEGTPADFVVFDAPTDTDAIRLGSRRLLVVRN
                     GRVVARTAPAETTVTWDGEEQPVTFLRP"
     gene            complement(6325..6630)
                     /locus_tag="VII50_08760"
     CDS             complement(6325..6630)
                     /locus_tag="VII50_08760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5050979.1"
                     /translation="MFSDMLLRRRARVDGFLFDTKHNPWAGWVAMGVAMVVSIWMFSA
                     QTKYLAPIPKAHPGLGDITFEVGFVLAAVLYAIFYQLQGGRRSETLVIPEYGAGPRK"
     gene            complement(6673..>8338)
                     /locus_tag="VII50_08765"
     CDS             complement(6673..>8338)
                     /locus_tag="VII50_08765"
                     /inference="COORDINATES: protein motif:HMM:NF024905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HEY5050980.1"
                     /translation="EPVEENTEAAEQAVAKLKGTYLATPFRPTGATLSEQAIVQLVEE
                     IDWLLTLTAKHDDLPDDGYARAATPDRELLTLSVAALHDAATALIDADAGSTKKVACK
                     PCDEPALVIDLELLASLRMQAATATAQLVESWSAANIDADADVLECAFHARAVATATA
                     AAASSALVASGNADHDAVRAEQARWYGAADAPQSGIGEITQQALAQTSLRSVWLRNSL
                     RGAAGVALAVLIAKVTGVQHGFWVVLGTLAVLRSNALGTGAIALRAIAGTSLGFIVGG
                     ALLVGLGDSTTALWIVLPFAVLLASYAPLRISFLAGQAAFTVLLAVLFNIVQPTGWRV
                     GLVRVEDVAIGCAVSLVAGFLLWPRGAGPVVSADLADAYRTGTAYLSSAVHSLIGTHA
                     TLGQEAVSESAAAVATVASRRLDVAFRVYLAEQGTKLVHRDELAALVGGASRLLLTAN
                     SLATLPLRTGRATRDDELFLDGELHNLTTALENIADELSSSSTPPPSDRMSTTGSPES
                     VCARWIHHHLSHLQAGVDSLTQASAVVRSGVLNKHPVVVSSASSSR"
BASE COUNT         1350 a         2659 c         2842 g         1487 t
ORIGIN      
        1 caatggtgat gaggccctgc tgctcgagcc attcgacggc cggccctccg gcgatccgga
       61 gcagggtgtc gaagtccagt ggttcgctga gcgcgatcaa ctcgagagct tctcggcgtc
      121 catcatcgag agcactaagc cggagactga cgagctcggc taattgggcg tcggccggca
      181 acgtgccgtc gagttgccag acgccgcggt gctggcgcag cgagtcgttg gcgagggcga
      241 cccggatcag ctcccggagc acgagcgggc tgccgaggct gaggcggtac atgcgctgcg
      301 ccgcgcgcgc cgaaacctgc ccacccagcg ttgattcgag caacgcagca gtgcccgcct
      361 cgtcgagcgg attgacctcg atggcgagca tgttgtcgcc gtccgcgaag ttgcgcagcg
      421 cgggcggcag gtccgccgcg acccgcgtga cgaggatgac ctgaaggcct tggcggtcca
      481 taagctgccg caaaaccaag ccggacaaac cgtcgagcag atgcgcgtcg tcgacgagca
      541 gcagcgttat tccactcgcg gccagggtcg ccgctatcgc ctcgcgggcg cgggcgagga
      601 gcaggccgtt ctccgccggg gcctcggtac cggtgaggaa cggactcagc gcggcgagtg
      661 gcacttcgtg cgatgaggtc gtgccgaaga tggccttgga cggccgcccg gcggcgtccg
      721 cattggcaat cgcagccttc gcaagccacg tcttgccaat cccgatcggg ccggtgagca
      781 gtaggttgcg accggccagc accgtgctcg tcagctcggc cagcaggacc gcgcgatcgg
      841 gcgccgccac cacctgggtg gacgccgggc cagatcctgg attcacgacg taacggtatg
      901 ccgcgacgaa gtcgcaggct acgtagggaa ctactgatgt agccgactac ggatgccgca
      961 cgcgcgcgcg acccgcacgc ccggctccga ttccacacac attcaggttc tttgagccca
     1021 ggaagctgga tgtgtgagga gtcgagccgc atctgtgagg aaggccaggt ggggcgggct
     1081 cgaggtgacg ggcgagccga gtcgtgcata tagttgcttt tagtcaagca actatcacgg
     1141 gagccacagt tgcctagccc gcattacaag tgggtcgcgc tgtcgaacac cacgctcggc
     1201 atgctcatgg cgacgatcaa ctcctcgatc atcttgatct cactgccagc gatcttccgc
     1261 ggcatcggga tcaacccact ctcgccgggc aacgttggct acctgctctg gctgatgatg
     1321 ggctacatgc tcgtcacggc cgtgctggtc gtgagcctgg gccgcctcgg tgacatgttg
     1381 ggtcgggtcc gcatttacaa cgcgggcttc gcggtgttct cggtcgcttc gatcatcttg
     1441 gcgctcgacc cgatgcgcgg tgggggcggc gcgttgtggc tcgtgttatg gcggctcttg
     1501 caaggcgtcg gcggcgcgat gctgttcgcg aacgcgagcg cgatcattgc cgacgcgttc
     1561 ccggtcgaac agcgtggcct cgcgatcggc atcaaccagg tcgccgcgat cgccggctcg
     1621 ttcatcggac tgatcgcggg cggcctgctg tcggtcgtcg attggcgtct ggtgttctgg
     1681 gtctcggttc cggtcggtgt gttcggcacg gtctgggcgt acaagagcct gaaagataat
     1741 ggacgtcgcg tccaagcgcg catcgactgg tggggcaaca tcacgtttgc ggtaggactc
     1801 acagccgtcc tcgcggcgat cgtgtacggc atccagccat acggcggtca taccatgggc
     1861 tggacggcgc cgaaggtggt cgtcgcgttc gtcgcaggca tcgggctact cgccgcgttc
     1921 ggatggatcg aactgcactc tgattcgccg atgttcaatc tgcggttgtt ccggatccgc
     1981 gcgtttgcct tcggcggttt cgcgggactg cttgccgcaa tctcgcgtgg cggcatgcag
     2041 ttcatgctca tcatctggct gcagggaatc tggttgccgt tgcacggtta caagtacgag
     2101 gcgaccccgc tgtgggccgg catctacttg ttgcctctaa cggttggctt cctcatcgcg
     2161 ggcccggtct cgggctactt gtccgaccgg cacggcgcgc gaacgctgtc gacgctcggc
     2221 atggtcgtgg tggcaggcag cttcgccggt ctgctgctcg tgccgaccaa cttcgcgtac
     2281 tggatctttg ccgtgctggt actgcttaac ggcatcggtg gcgggttgtt ctcggcgccg
     2341 aactccaccg ccatcatgaa cagcgtgccg cccgaacaac gcggtggcgc agcaggtgtg
     2401 caggcgacgt tcctcaactc cggcatggtg ttgtcgatcg gcatcttctt ctcgttgatg
     2461 atcgtcggtc tcgcgaacac gttgccttcg gcaatgttca agggactgac cgccaacggg
     2521 gtcagcgctg ctcaagcgca cacgattgcg aaccagccgc cggtcggcag cctgttcgca
     2581 tcgtttctcg gattcaatcc gctgcaaacg ttgctcggtt cgcaatcggc tgcacacgtg
     2641 tcgaacgcgc aatgggccac gttgacaggt aagcacttct tcccgaacct gattgcgtca
     2701 ccgttccatc acggcctcgt tatcgtgttc gcagtggcga tcgggctttc gattgtcggc
     2761 gcgatcttct caatgctgcg cggccgtcgc tacattcacg gcgacgacgt cgaggtcgtg
     2821 cacgcagacc acgagcaccg cggcgatcca gcgcttgctt ccggtgcggt gccaggggag
     2881 ctggcgttgc aggacgaccg cacatgacgc ggcccacagc aacagtcagt ccggacgccg
     2941 aaactgcggc gcgcttgcga ctggtgattt cgcgactcgc ccgtgcggtg cgccaacacg
     3001 gtgccgccgg cctcacgccg tcccagctct cggctttggc gacgatcgaa gagctcgggc
     3061 ccatgcgcat gagcgatctc gccgggcacg aactcatcgg tgcgccggtc gcgacccgcg
     3121 tcgtcgcgac gctgcaggag ctcggactcg tccagcgcgc cggcggctcg accgacgggc
     3181 gggtctctct catcgagctc agcgacgccg gccgcgaaac cctcgccgag ctgtggagcg
     3241 aacgagccgc cggcctcacg gcgcgcatcg agcgactcaa gcctgaacag gtcgccgtcc
     3301 tgacctctgc cttgccggtg ctcgaaacgc tggtacgcga ccgcgtcatc gtctgagccg
     3361 atccgacgcc cgaatcggcg catcggtaac cggggctttg cccagcggca acgttcgcgt
     3421 cacatcggcc tgccattgtc gatctcgagc ccgaggtgga gctcagcatg gcgaaggcaa
     3481 aggggagcga catgatcacg aatgcgagaa ggctgcagct cgtacgtccg tcggtgacgt
     3541 tggaagaggt gtccagcgcc gccggagtat cgaagtcgac cgcgtcgcgt gcgctctgca
     3601 acgagccgaa cgtcaaggcg gagacccgcg cccgggtgca ggcgatagcc gcgggactcg
     3661 gctatgtgcc aaacgaagct gctcgtgcgc tggcggcccg tcgccaggtt gtgatgagcg
     3721 gtacgcgcgc gggctagcaa gagtgtcaca gtagaaagat gcggggacgg cgatgggctg
     3781 gcgtaagcct ggcagcgttg gggatgctgg cgggttgttc gcaagcaggg agcgcggtgg
     3841 ggtcgcgcgc atcgtcgggg ttaccggggt tgtcgtctga atcgttgagc gcgtcgtcgg
     3901 tcgtgccgtt gtcgagtgcg caggtgggag cacctgcagc gacgcggccg gcgccgccac
     3961 cagcgtcacc cactccgggc cagtcgttga cggctacccc ctcggccacg gcactacctt
     4021 tgaccggggt cgcgtacgtc gatgtgtccg tcgcgacgtt gtggcacagt ccgagttcgc
     4081 cgcgcgctat cgacgcggcg gagttgcggc aacccgccgg ggtggtcgcg tggttgggtg
     4141 cgatgacgac ggcccagcgg ctcgcattgt acggacgcgt tgatagccag ctcttactcg
     4201 gcgagaaagt ggtcgtcgac aggcagcagg ggacgtgggc acacgtcgtc gtcccggatc
     4261 aaccgacgcc acttgatgcg cgcggctatc cgggctggat tcccgctgcc cagctgacgt
     4321 cgcgggcacc ggccgcagcc gcgaacgatg tcgtggtgct cgcgccgtcg gcgtggttgt
     4381 acgggcccga tcaagtgacg cgcgttgccg aggtcagcat ggcgacccgt ttgcccgcac
     4441 tctgggccga cacgtcgtgg gtgcacacgc agttgccgga cggtcgggcc gtgctgttac
     4501 gcgcggccga cgccgccgtt atggcgcatc tcggggctcc gttgcccgcg acggctgctg
     4561 acgtcgtccg gacagcgaag ctgttcttgg gtttgcagta tttatggggt ggcacgtcgg
     4621 cgttcgggtt cgattgctcg gggctcacgc atttggtgtt cgagctgcgg gggatcacga
     4681 ttccgcgcga tgccgacgca caggcgcggg tcggacaagc cgtcgcccgc gccgatctgc
     4741 agcccggcga tctggtcttt ttcgcgagca acgggctggt gcaccacgtc ggaatctacg
     4801 tcggcaacaa catgatggtg agctcgttac agaccgggag tccggtggag tattcgtcgc
     4861 ttgctgccca accgttcgcc agcgaataca gcggcgcccg gcggctggtc ggctgatagg
     4921 ttctgcgcgt gctcgacctt ctgatccgaa acgcgcaggt ccgcggcacc gagggcctga
     4981 ccgacatcgg cgtgcgcggc ggcgtcttcg cgccacccga ggctgacgct ccggaggttc
     5041 tcgatgtcgc aggtgcgctg gtcacgccgc cgctcgtcga gccgcacatc cacctcgacg
     5101 cggtgctgac cgtcggccag ccgcggccga acagatcggg ctcgctgttc gaaggcatcg
     5161 cggtgtggag cgagcgcgtc aaggacctta cggtcgacga cgtgaaatcg cgagtgcggg
     5221 aggtgttgcg ctggcagctc gccaacggcg ttcagcatgt gcgcagccac gtcgacgtgt
     5281 gcgatcccga actgaccgca ttgcgcgcgc tgatcgagat tcgtgaagaa gcgcgcgggc
     5341 aggtcgatct gcagttggtg gcgtttccgc agctcggcat cgtgtcgttc gacggcggtc
     5401 gcgaactcat gcgcaaggct gtcgaactcg gtgccgatgt cgtcggcggc atcccgcact
     5461 acgagctcac gcgcgaagat ggtgtcgagt cggtcaagtt ctcctttgcg ctagcggaag
     5521 aattcggcct gcgcgtcgac atccattgcg acgagacgga cgacgaccac tcgcgcttcg
     5581 tcgaagtcat ggctgccgag accattcgtc gttgcatgtc ggggcgggtc accgcgagtc
     5641 acaccacggc gatgcactcg tacaacgcgg cgtatgcgta ccggctcgtc aacaacatca
     5701 agcgcgccgg catgcacatg gtgacgaacc cgctcgacaa cgcggtgctg cagggacgct
     5761 tcgacaccgg accgatccgg cgtggccaca cgcgggtgaa gcagctgcaa gaagccggcg
     5821 tcaacgtgtg catcggccac gactcggtga tggatccctg gtaccccctc ggttacggcg
     5881 atccggtgca ggcagcgttc gtcttcgtgc acctcggtca ttacagcggg gacgacgagt
     5941 tgcggcggct cctcgacatg gtgaccgtca atccggctgc ggcactgggc atcgagggct
     6001 acggcatcgt cgaaggaacc ccggccgact tcgtggtgtt cgacgcgccc accgacaccg
     6061 acgcgatccg gctgggatca cgccggcttc tcgtcgtccg caatggtcgt gttgttgctc
     6121 gcaccgcccc cgccgagaca accgtgacct gggacggcga agagcaaccg gtgaccttcc
     6181 tccgcccgta gccccgctag ggttgccgaa gcgggacggg gaagcgcggc catcgactgg
     6241 gcgcaaacgc acgcgacacg aggccgcggg tgcgtttgtc cccactcgac gcagtgccgc
     6301 aggtcgacgc tcgcccgcgc cgcgctactt cctcgggccc gcgccgtact cggggatgac
     6361 cagcgtctcg gaacggcgtc cgccctgcag ctggtagaag atcgcgtaca gcacggccgc
     6421 gagcacgaag ccgacctcga aggtgatgtc gccgagtccg gggtgcgcct tcgggatcgg
     6481 tgcgaggtac ttcgtctggg cggagaacat ccagatgctg accaccatcg cgacgcccat
     6541 cgccacccag ccggcccacg gattgtgctt ggtgtcgaac aggaatccat cgacgcgggc
     6601 acgacggcgc agcagcatgt cggagaacat gacgcctagc caccgcgcta tggccggtgt
     6661 cgtgtagacc ggtcaacgcg atgatgaagc cgatgacacc acgaccgggt gcttgttcaa
     6721 cacccctgat cggacgacgg cagaggcttg ggtcagcgag tcgacgccag cctgcaaatg
     6781 gcttaggtgg tgatgaatcc atcgcgcgca tacagattcc ggcgaccccg tagtgctcat
     6841 tcgatcggac ggcgggggtg tgctgctcga tgacagttcg tcggcgatgt tctcgagtgc
     6901 ggttgtgagg ttatgcaact cgccgtccag aaaaagctcg tcgtcacggg tagcgcgacc
     6961 tgttcgcagc ggcaacgtcg cgagggaatt cgcagtaagg agcaaccggc tcgcgccgcc
     7021 gacgagcgct gcgagctcgt ctcggtgcac cagcttggtg ccctgctcgg cgaggtaaac
     7081 ccggaacgcg acgtcgagcc gccgcgaggc gacggtcgcg actgccgccg ccgactcact
     7141 taccgcctct tgaccgagcg tcgcgtgtgt cccgatgagc gagtgcaccg ctgacgacag
     7201 gtacgcggta cccgtgcgat atgcgtcggc gagatcggcg gagaccaccg gtccggcgcc
     7261 gcgtggccac agcaggaacc cggcaaccag gctgaccgcg caaccgatcg cgacgtcctc
     7321 gactcgcacc aggccgaccc gccagccggt cggttgcacg atgttgaaca ggacggcgag
     7381 cagcaccgtg aatgcggcct gaccggcgag gaaggagatc cttaaggggg cgtaactcgc
     7441 gagcaggacg gcgaacggca acacgatcca cagcgctgtc gtgctgtcgc cgaggccgac
     7501 gagaagagcc ccgcccacga tgaatccgag cgacgtgccg gcgatcgcgc gtagcgcgat
     7561 ggctccggtg ccgagtgcgt tcgaccgcag cacagcgagc gtcccgagca ccacccaaaa
     7621 gccgtgctgc acgccggtca ccttcgctat tagcactgcg agtgcaacgc cggccgcacc
     7681 gcgcagactg ttgcgcagcc acaccgaccg caggctggtc tgcgcgagcg cctgctgggt
     7741 gatctcgcca attccggatt gcggcgcgtc ggcggctccg taccagcgtg cttgctctgc
     7801 gcggaccgcg tcgtggtcag cattgccaga agcgacaagg gcggacgacg ccgcagcggc
     7861 tgtcgcggtg gcgaccgccc gagcatggaa ggcacactcc agcacatcgg cgtccgcgtc
     7921 gatgtttgca gcggaccagg attcgacgag ctgcgccgtc gctgtcgccg cttgcatgcg
     7981 caggctggcg agcagctcga ggtcgatgac caacgccggc tcgtcacacg gtttgcaggc
     8041 cacctttttg gtgctgcctg catcggcatc gatgagtgca gtggccgcgt cgtgaagcgc
     8101 ggcgactgac agcgtcagca gttcgcgatc cggcgtcgcc gcgcgcgcgt acccgtcatc
     8161 gggcaggtcg tcgtgctttg cggtgagggt gagtagccag tcgatttcct cgaccagttg
     8221 gacaattgcc tgctcggaca gcgtcgcccc cgtcggtctg aatggggtgg ccaggtaggt
     8281 acctttgagt ttcgcgacgg cttgttctgc tgcctctgtg ttttcctcga cgggctcg
//
LOCUS       DATCBJ010000433         6150 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_7732, whole genome shotgun sequence.
ACCESSION   DATCBJ010000433 DATCBJ010000000
VERSION     DATCBJ010000433.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 6150)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6150)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6150
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7732"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1527)
                     /locus_tag="VII50_08770"
     CDS             complement(<1..1527)
                     /locus_tag="VII50_08770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HEY5050981.1"
                     /translation="MKEPSTTAEAGGITVTINGTDLVMAARNATTTLVDAGVPKSLLS
                     KDSGLWGPDAAALAATRLGWLDLPSSSRQYIPELLDLRARLTADGIDHVVLAGMGGSS
                     LAPEVIAATAGVRLTVLDTTDAGQVAAAIADRLEHTVLVVSSKSGGTLETDSHRRVYE
                     QAFRDAGIDPVQRIVVVTDPGSPLEQASRAAGYRVFLADPNVGGRYSALSAFGLVPTA
                     LAGVDVAGLLDDAAALLPALGGDDDNPGLTLGAVLGEAGRAGRDKVVIADFGSRHPGF
                     GDWAEQLVAESTGKQGTGLLPVVVESVESPGFGEGPDRHLMTLGQHLHIDGTTISGSL
                     GAQFMVWEYATAVAGRVLGIDPFDQPNVQESKDNTGKILDEAGNGPLPEGRPIFTDGA
                     VEIHTDDPILLGGAHDLSGALSALLRAVPDRGYVAVMAYLDRHADSAAALLRRSLARH
                     TERPVTFGWAPRFLHSTGQYHKGGPQTGAFLQITGVVDRDLAIPGRPFGFARLQMAQA
                     L"
     gene            complement(1533..2654)
                     /gene="tal"
                     /locus_tag="VII50_08775"
     CDS             complement(1533..2654)
                     /gene="tal"
                     /locus_tag="VII50_08775"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004801 - transaldolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HEY5050982.1"
                     /translation="MASETDPLGQLSAAGVAVWLDDLSRERLSTGNLSDLIRDRHVVG
                     ITTNPTIFQKAISGADAYAAQLHDLATRGVDVGEALRLITTSDVRWGCDVLRPAYDAS
                     NTVDGRVSIEVDPRISGDAAKTIAEARSLWWMVDRPNLFIKIPATVASLPAISQALAE
                     GISVNVTLIFSLERYAKVIDAFFDGLERARAAGLDLAGIGSVASFFVSRVDTEIDKRL
                     DKIGSDEAKALRGKAAIANARLAYELYEENFSGERWESLAASGAKAQRPLWASTGVKD
                     PAYDDTMYVVDLVADHTVNTMPEPTLQAVHDHGVISGDSIRAHYFDARVVLDKLAAVG
                     IDYDDVVLTLEREGVEKFEASWVDLIAAVQKELEQKAGS"
     gene            complement(2676..4811)
                     /gene="tkt"
                     /locus_tag="VII50_08780"
     CDS             complement(2676..4811)
                     /gene="tkt"
                     /locus_tag="VII50_08780"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HEY5050983.1"
                     /translation="MANHQTGGRSGAVEWSDVDAKTVDVIRALAMDAVEAAGSGHPGT
                     AMSLAPAAYLLFQRLLRHDPTEPGWLGRDRFVLSCGHSSLTLYIQLYLSGYGLTLEDI
                     SLLRTWGSLTPGHPEHGHTLGVETTTGPLGQGVGNAVGMAMAARRERGLFDPDAAPGE
                     SPFDHHIYCFASDGDLEEGVSGEASSIAGTQRLGNLTLLWDDNHISIEDDTNIAFTED
                     TAARYAAYGWHVQRVNWLDDDGSYVENVGALYEAFLAAQQVTDRPSFIALRTIIGWPA
                     PNKQNTGKAHGSALGAAEVAATKTVMGMDPDKKFDVPAEVLEHARGVIARGQTLRGEW
                     QQSYDAWAAANPESVALLERMSRRRLPEGWESALPVFEPDQKGIATRAASGKILTAIA
                     PILPELWGGSADLAESNNTTPEGQPSFLPEDRQSKAFPGGPYGRVLHFGIREHAMGSI
                     LNGIALHGGTRPYGGTFLTFSDYMRGAVRLAAIMKLPVTYVWTHDSIGLGEDGPTHQP
                     IEQLAALRAIPGLDVVRPADANETAVAWRTILENTDRPAGLILSRQNLPIVDRTVFAS
                     AEGVARGAYILADASSGKPDAILIATGSEVPIAIAARELLEAQGMPTRVVSMPCQEWF
                     MEQDAAYRQLVLPPDVKVRASIEAAVTMGWRDFVGDHGEMLGLNHYGASAPGAILFEQ
                     FGLTADRLVAAVHSSLSKVGGTKGSTTGN"
     gene            5028..5951
                     /locus_tag="VII50_08785"
     CDS             5028..5951
                     /locus_tag="VII50_08785"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0048034 - heme O biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HEY5050984.1"
                     /translation="MTVLAQTVPGTPTGRRPARQLVSAYVALTKPRIIELLLVTTVPA
                     MILARRGMPSWWLVVATLVGGTFAAGSANTINCFVDRDIDKVMRRTSRRPLARARVRP
                     AEALRFGIVLGVVATLLLGLTVNWASALLADLAIMFYVFVYTLGLKRRTPSNIVIGGA
                     AGCFPVLVGWSAVTGGVGWPAIVLFGVIFAWTPPHFWALAMKFRDDYAAAGVPMLPVV
                     ADATTVTRKIVGYSYVMVAVSFVLVPVGHAGLLYLVAAGALGGWFLVEAHRLSARAKA
                     GEDPKPMRLFHLSVTYLTLLFAAIAIGQFVR"
     gene            complement(5944..>6150)
                     /locus_tag="VII50_08790"
     CDS             complement(5944..>6150)
                     /locus_tag="VII50_08790"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEY5050985.1"
                     /translation="RSLVDFAEGCDARVVAEGVETAADAAVLCGVGVDYGQGWHYGRP
                     GPAADLPAAAAAPVGALRPLLTAT"
BASE COUNT          958 a         2000 c         2082 g         1110 t
ORIGIN      
        1 caacgcctgc gccatttgta accgggcgaa cccgaatggc cggccgggga tggcgaggtc
       61 acgatcaacg acgccggtga tctggaggaa cgccccggtc tgcggaccac ccttgtggta
      121 ctgcccggtg gagtgcagga atcgcggtgc ccagccgaac gtcactggcc gctctgtgtg
      181 ccgcgcaaga gatcgacgca gcagcgcggc ggctgagtcg gcgtgccggt cgaggtaggc
      241 catcactgcg acatagccac gatcgggcac cgcgcgcagc agcgcagaca acgctccgct
      301 gaggtcgtga gcgccgccta gcaggatggg atcgtcggtg tggatttcga ctgcgccgtc
      361 ggtgaagatt ggccttccct cgggcagcgg tccgttgccg gcttcatcga ggatcttgcc
      421 ggtgttgtcc ttggactcct gcacgttcgg ttggtcgaac ggatcgatgc ctagcacgcg
      481 tccggccacc gcagtggcgt actcccacac catgaactgc gcaccgagcg agcctgagat
      541 cgtcgtgccg tcgatatgca gatgctggcc aagcgtcatc agatggcggt cgggaccctc
      601 gccgaagccg ggggactcca ccgactcgac aaccacagga agcaggccgg tgccctgctt
      661 cccggtcgac tcggcgacca gttgctctgc ccagtcgccg aagcccggat ggcgtgaccc
      721 gaagtcggcg atcactacct tgtcgcgacc tgcccggccg gcctcgccga gcacagcacc
      781 gagcgtcagg ccggggttgt cgtcgtcgcc cccgagggcg ggtaacaatg cggcggcatc
      841 atcgagcaac cccgcaacgt cgacccccgc gagcgcggtc ggcaccagcc cgaatgccga
      901 cagcgcgctg tagcgacccc cgacatttgg gtcggcgaga aacacccggt agccggccgc
      961 tcgcgacgcc tgttctagcg gtgagccagg atcggtgacc accacgatgc gctgcacggg
     1021 atcgatgccg gcgtcgcgaa acgcctgctc atagacgcgc cggtggctat cggtctcgag
     1081 cgtgccgccg ctcttgctcg acacaaccag cacggtgtgc tcgaggcggt cagcgatggc
     1141 ggcggccacc tgtcctgcgt cggtggtgtc gagaacagtg agccggacgc cggcggtggc
     1201 cgcgatgact tctggtgcga gcgacgatcc gcccatgccg gcaaggacca cgtgatcgat
     1261 gccgtcggcc gtcaaccgag cgcgcaggtc aagcagttca gggatgtact gccgggaact
     1321 gcttggcagg tcgagccacc cgagccgggt cgcagcgagc gcagccgcgt cggggcccca
     1381 cagccccgaa tccttgctga gcaagctttt cggtacgccc gcgtcgacca aggtggtcgt
     1441 cgcgttgcgg gcggccatga ccaggtcagt gccgttgatc gtcacggtga tcccgccggc
     1501 ctcggcggtg gttgagggct ccttcatgcc ggtcaacttc ctgccttctg ctcgagttct
     1561 ttctggacgg cggcaatgag gtcgacccac gaggcttcga acttctcgac accctcgcgt
     1621 tcgagagtga gcacgacgtc gtcgtagtcg atgccgacag cggcgagctt gtcgagaacc
     1681 acgcgcgcat cgaagtagtg cgcgcgtatc gagtcgcctg agatgacacc gtgatcgtgg
     1741 acggcctgca gggtcggctc cggcatcgtg ttgacggtgt ggtcggcaac cagatcgacg
     1801 acgtacatcg tgtcgtcgta ggccgggtct ttgacacccg tcgacgccca cagtggacgc
     1861 tgcgctttcg caccggaagc ggccagcgat tcccagcgtt cgccgctgaa gttctcctcg
     1921 tacaactcgt aggcgagccg cgcgttggcg atggcggctt tgcctcgaag cgccttggct
     1981 tcatctgagc cgatcttgtc gagacgcttg tcgatctccg tatcgacgcg ggaaacaaag
     2041 aacgaggcga ccgagccgat accggcgagg tcgagccctg ccgcgcgcgc tcgctcgaga
     2101 ccgtcgaaga acgcgtcgat caccttcgcg tagcgctcga gggagaagat cagtgtgacg
     2161 ttgacactga tgccctcagc caatgcctgg ctgatcgcgg gcaagctcgc gaccgtcgcc
     2221 gggatcttga tgaacagatt cgggcggtcg accatccacc acagcgaccg ggcttcggcg
     2281 atggttttcg cggcgtcgcc ggagatgcgc gggtcaacct cgatcgacac tcggccgtcg
     2341 acagtgttgc tggcgtcata cgccggacgc agcacgtcgc aaccccaccg gacgtcggac
     2401 gtcgtgatca gccgcaacgc ctcgccgacg tcgacccccc gtgtcgccag atcatggagc
     2461 tgagccgcat aggcgtcggc accgctgatt gccttctgga agatcgtcgg attggtcgtg
     2521 atgccgacga cgtggcggtc gcggatcagg tcgctgaggt tgccggtgct cagtcgctcg
     2581 cgactgaggt cgtcgagcca cacggcgacg ccggcggcac tgagttggcc gagcggatcg
     2641 gtctcggatg ccatgggttg ctcctttgcc tgctgttaat tgcctgtggt gctgcccttt
     2701 gtcccgccga cctttgacag gctcgagtgc acggcagcca ccagacggtc ggcggtcagg
     2761 ccgaactgct cgaagagaat tgctccaggt gcgcttgcgc cgtagtgatt gaggccgagc
     2821 atctcgccgt ggtcgccgac gaagtcgcgc caacccatgg tgaccgccgc ttcgatcgac
     2881 gcgcgaacct tgacatcggg ggggaggacg agttggcggt acgccgcgtc ctgctccatg
     2941 aaccactcct ggcacggcat cgagaccact ctggtcggca tgccctgtgc ttcgagaagt
     3001 tcccgcgctg ctatggcgat cggcacctcg ctgccggtgg cgatgagaat cgcgtcgggt
     3061 ttgccggagc ttgcgtcggc aagaatgtac gcgcccctgg cgacaccctc ggccgacgcg
     3121 aagacggtgc ggtcgacgat gggcaggttc tgccggctca ggatcaggcc ggccgggcga
     3181 tccgtgttct cgaggatcgt gcgccaggcg accgctgtct cgttcgcatc ggctgggcgg
     3241 acgacgtcga gcccggggat cgcgcgtagc gcagcgagtt gttcgatcgg ctgatgcgtc
     3301 gggccatcct cgccaagacc gatcgagtcg tgggtccaga cgtaggtcac gggcagcttc
     3361 atgatggcgg cgagtcggac ggcgcctcgc atgtagtcgc tgaaggtgag gaaggtgccg
     3421 ccgtaggggc gggtgccacc gtgcagggcg atgccgttca gaatcgagcc catcgcgtgt
     3481 tcgcggatgc cgaagtgcag aacgcgaccg taaggtccac cggggaaagc cttggactga
     3541 cggtcttcgg gcaggaacga cggctggccc tcgggcgtcg tgttgttgct ttcggcgagg
     3601 tcggccgaac ctccccagag ctcgggcaga attggtgcga tagccgtgag gatcttgccg
     3661 gacgcggcgc gcgtcgcgat tcctttctgg tccggctcga agaccggcaa cgcgctctcc
     3721 cagccctcgg gcaagcggcg ccgcgacatg cgctcgagga gtgcaacact ctcggggttg
     3781 gctgcggccc acgcgtcgta gctctgctgc cactcgccac gcagcgtctg gccacgggcg
     3841 ataacacccc gcgcgtgctc cagcacctct gccggtacgt cgaacttctt gtccggatcc
     3901 atgcccatca cagttttggt tgcagcaacc tccgcggcac cgagcgcaga accgtgggcc
     3961 ttgccggtgt tctgcttgtt cggggctggc cagccgatga tcgtgcgcag cgcgataaac
     4021 gacggccgat cggtgacttg ctgggcggcc aggaacgcct cgtacaacgc gcccacgttc
     4081 tcgacgtagg agccgtcgtc atcgagccag ttgacccgct gcacgtgcca gccgtacgcc
     4141 gcgtagcggg cggcggtgtc ctcggtgaag gcgatgttcg tgtcgtcctc gatggagatg
     4201 tggttgtcgt cccagaggag ggtgaggttg ccgagtcgct gcgtgccggc gatgctcgac
     4261 gcctcaccac tcacgccctc ctcgaggtcg ccgtcggacg cgaagcagta gatgtgatgg
     4321 tcgaaggggc tctcacctgg cgcggcgtca gggtcgaaga ggccgcgttc gcgccgcgcc
     4381 gccatcgcca tgccgaccgc gttgccgacg ccttggccaa gcggcccggt cgtcgtctcg
     4441 acaccgaggg tgtgaccgtg ctcagggtgc ccaggggtca ggctgcccca cgtgcgcagc
     4501 aagctgatgt cctcgagggt caagccgtac cccgacaggt acagctggat gtacagcgtc
     4561 aggctcgagt ggccacacga cagcacgaac cgatcacgac cgagccaacc tggctcggtc
     4621 gggtcgtggc gcaacagtcg ctggaacagc aggtaggccg ccggtgcgag gctcatggcc
     4681 gtgccggggt gtccggaccc tgcggcttcg accgcgtcca tcgcgagcgc cctgatgacg
     4741 tcgacggttt tcgcatcgac gtcggaccat tcgacggcgc cggaacggcc gcccgtctgg
     4801 tgattcgcca tgggcgttgt cactgctcct cttgccgggg cctcagtcgc ggggagatgc
     4861 catggaatcg atctgacgaa tgtcacgaca tctccgactg tcaccctacc gatgcagccg
     4921 ccgtccggta gacccatggg cctcgccgcg accgcccagg accagctgcg catattggtg
     4981 ctcggtagga tgcggctcgt gcactcggcg tcggtgggga tggtctcgtg accgtgctgg
     5041 cgcagaccgt tccgggcacc ccgaccggac gacggccggc tcggcaactg gtcagtgcat
     5101 acgtcgcttt gaccaagccg cgcattatcg agttgcttct cgtgaccacg gtgcccgcga
     5161 tgatcctggc tcgtcgcggc atgccctcgt ggtggctggt tgtcgccacc ctggtgggcg
     5221 gtaccttcgc cgcgggcagt gcaaacacga tcaactgctt cgttgatcgc gacatcgaca
     5281 aggttatgcg ccgcacctca cggcgtcccc ttgcacgtgc tcgtgtacga cccgccgaag
     5341 cgctgcgatt cggcatcgtt ctcggggtcg ttgccacctt gctgctcgga ttgaccgtga
     5401 actgggcgtc ggcgctcctt gccgacctcg cgatcatgtt ctacgtcttc gtctacacgc
     5461 tgggcctgaa gcgccggacg ccgtcgaaca ttgtgatcgg cggcgcggcc ggttgctttc
     5521 cggttttggt cggatggtcg gcagtgaccg ggggagtcgg ctggccggcg atcgtcttgt
     5581 tcggcgtgat cttcgcctgg accccgccgc acttctgggc gctggcgatg aagttccgtg
     5641 acgactacgc agcagctggt gtgccgatgc ttccggtcgt tgctgacgcg accactgtca
     5701 ctcgcaaaat cgtcggctac tcctacgtca tggtggccgt gtcgttcgtg ctcgtgcccg
     5761 tcgggcacgc cggactgctc tacctcgtcg cggccggcgc tctcggcggc tggttcctcg
     5821 tcgaggcgca tcggctctcg gcgcgcgcga aggcaggcga ggatcccaag ccgatgcggc
     5881 tcttccacct gtcggtcacc tacttgacgc tgctgttcgc cgcgatcgcg attggtcagt
     5941 tcgtcaggta gcggtaagta gtggccgcag cgcgccgact ggcgccgccg cggctgctgg
     6001 aaggtcagcg gcggggccgg ggcgcccgta atgccagccc tggccgtagt cgacaccaac
     6061 gccgcaaagg acagctgcgt ccgctgcggt ctcgacgccc tcggcgacaa cacgcgcgtc
     6121 gcatccctcg gcgaaatcga cgagtgagcg
//
LOCUS       DATCBJ010000434         2285 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_222071, whole genome shotgun sequence.
ACCESSION   DATCBJ010000434 DATCBJ010000000
VERSION     DATCBJ010000434.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2285)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2285)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2285
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_222071"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..185)
                     /locus_tag="VII50_08795"
     CDS             complement(<1..185)
                     /locus_tag="VII50_08795"
                     /inference="COORDINATES: protein motif:HMM:NF016959.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HEY5050986.1"
                     /translation="MPLTAEDVKNKQFTPTRFKGGYDEKEVDDFLDEVEAELTRLVRE
                     NTDLRARLQVAEQAARQ"
     gene            complement(226..495)
                     /locus_tag="VII50_08800"
     CDS             complement(226..495)
                     /locus_tag="VII50_08800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HEY5050987.1"
                     /translation="MADVIVAFIVVMFIRLVFDYVQLFAREWRPRGVVLIALETCYSM
                     TDPPLKFVHRLLPPLRVGAFSADWTVAWIAVIGTAYILLSVVGSL"
     gene            complement(531..1091)
                     /gene="sepF"
                     /locus_tag="VII50_08805"
     CDS             complement(531..1091)
                     /gene="sepF"
                     /locus_tag="VII50_08805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0090529 - cell septum assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HEY5050988.1"
                     /translation="MAVMRKAMVYLGLVEDDAENYDEYDEYEAPAPVPAGRRAGTQVD
                     RGAPADIEYGRRGAAPDLRDRAPAYAGGRSSGGATAVAVAPMGWADEAAGPIRVTTLH
                     PRTYNEARAIGEHFREGTPVIMNLTEMDDADAKRLVDFAAGLIFGLRGSIERVTAKVF
                     LLSPQNVEVTAEDKQRIAEGGFFNQS"
     gene            complement(1137..1889)
                     /locus_tag="VII50_08810"
     CDS             complement(1137..1889)
                     /locus_tag="VII50_08810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HEY5050989.1"
                     /translation="MTGTTGSTSHARRDELADNLQRVRQRIAAACVAAERAVDEVALI
                     AITKTFPASDVALLAELGVTDVGENRDQEAAAKVAAVASICTDPGVLRWHFVGALQTN
                     KCPSVVRYASLIHSVDRLRLVDALGRAAHQTAARQTAVRCLVQVDLDETSRPGRAGAR
                     RDEVGAIADAIADDVRLELAGVMAVAPLRADPGSAFRQLAEIADEVRQRHPLAQIISA
                     GMSGDLESAVANGATHLRVGSALLGARRHSVR"
     gene            complement(1886..>2285)
                     /locus_tag="VII50_08815"
     CDS             complement(1886..>2285)
                     /locus_tag="VII50_08815"
                     /inference="COORDINATES: protein motif:HMM:NF014619.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="polyphenol oxidase family protein"
                     /protein_id="HEY5050990.1"
                     /translation="VLADPAAGLVGCLHCGRPGLVAGAVDAMVAAMRESGAQAIRAAV
                     GPTVCGSCYEVPADMAAAVVAAVPAAFSRARGGGSALDVRAGVIDQLRHAGVRVVRLV
                     GGCTREDPTTFSYRRDKVTGRMGALIWRAT"
BASE COUNT          396 a          809 c          716 g          364 t
ORIGIN      
        1 tgctgccgtg cagcctgctc tgccacctgg aggcgagccc ggaggtcggt gttctcccgc
       61 acgagccgcg tgagctcggc ttccacctcg tcgaggaagt cgtcgacttc cttctcgtcg
      121 tagccgccct tgaagcgggt aggcgtgaac tgcttgttct tcacgtcctc agcggtcagc
      181 ggcatcgggt cacctcgggg aatcgttggg tacgcgccgg acaaactata gggaacccac
      241 tacagataac agaatgtacg cggttccgat aacggcgatc caggcaactg tccagtctgc
      301 actgaaggcg ccgacgcgca gtgggggcaa tagacggtgc acgaatttca gcggtggatc
      361 ggtcatcgag taacacgttt ccaaagcgat aaggacaacg ccacgggggc gccactctcg
      421 tgcgaacagc tgcacgtagt cgaaaaccag cctgatgaac atgaccacga taaacgcgac
      481 gataacgtcg gccacgaccc gccacacagt gcccacgtcg tgccggccgc ctagctctga
      541 ttgaagaatc cgccttcggc gatccgctgc ttgtcctccg ccgtcacctc aacgttctgc
      601 ggcgacaaca ggaagacctt cgcggtcaca cgttcgatac tgccacgcag accaaagatc
      661 agcccagccg cgaaatcgac gagccgcttc gcgtcagcgt cgtccatctc ggtgagattc
      721 atgatcaccg gggtgccctc gcggaaatgc tctccgatcg cacgtgcctc gttatacgtc
      781 cgcggatgca atgtcgttac tcggatcggg ccggcggcct cgtcagccca gcccatcgga
      841 gcgaccgcca ccgccgttgc tcccccacta gaccggccgc ccgcgtaggc cggagcgcga
      901 tcacgaaggt cgggcgctgc gccgcggcgt ccgtactcga tgtcggccgg ggcgccacgg
      961 tcgacttgcg tgcctgcccg ccgacctgcc ggaaccggcg cgggcgcttc gtactcgtcg
     1021 tattcgtcgt agttctcggc gtcgtcctcg acgaggccga ggtagaccat cgccttgcgc
     1081 atgacggcca tccgtgtctc cctctggtcc cctcagacgt cggtttcgtc cggaccttac
     1141 cggacagaat gccgccgagc gcccagcaac gccgagccga cacgcaggtg agtcgcgccg
     1201 ttcgcgacgg ccgactcgag atcgccgctc attccggccg aaattatctg ggccagcggg
     1261 tggcgctggc ggacctcgtc ggcgatctct gcaagctgtc gaaacgccga accggggtcg
     1321 gcgcgcagcg gcgctacggc catcaccccc gcgagttcga gacggacgtc atcagcgatg
     1381 gcgtcggcaa tcgcgccgac ctcgtcgcgc cgtgcgccgg ctcggcccgg tcggctcgtc
     1441 tcgtcgaggt caacctggac gagacatcgg accgctgtct gccgcgcagc cgtctgatgg
     1501 gcggcacggc cgagcgcatc gacgaggcgc aaccggtcca ccgaatgaat caggctcgcg
     1561 tagcggacga cagaagggca cttattcgtc tgcaacgcac cgacgaagtg ccatcgcaga
     1621 acgcccggat ccgtgcatat ggaggcgacg gcggcgacct tggcggcggc ctcctgatcg
     1681 cggttctcgc ccacatccgt gacgccaagc tcggcaagca gcgccacgtc tgatgccggg
     1741 aatgtcttcg tgatcgcaat gagcgcaacc tcatcgaccg ccctttcagc ggcgacgcac
     1801 gccgccgcga tgcgctgtcg aactcgctgg aggttgtccg ccagctcgtc ccgtctcgca
     1861 tgcgaggtcg acccggtcgt cccggtcacg tcgcccgcca gatcaaggca cccatccgac
     1921 cggtgacctt gtcgcggcgg taggagaagg tggtcggatc ctcccgcgtg catcctccga
     1981 cgaggcgcac gacccgcacg ccagcgtggc gcagctgatc gatcacgccg gcccgtacgt
     2041 cgagggccga gccgccaccc cgggcacgcg agaacgcggc aggtacggcg gccactacag
     2101 ccgctgccat gtccgcagga acctcgtagc aactcccgca cacggtcggt ccgaccgccg
     2161 cacggatcgc ctgcgcgccg ctttcgcgca tcgcggcgac catcgcgtcg acggcgccgg
     2221 ccaccaggcc cggccggccg cagtgcaggc agcccaccaa tccagcggcg ggatcggcca
     2281 ggacg
//
LOCUS       DATCBJ010000435         4913 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_301581, whole genome shotgun sequence.
ACCESSION   DATCBJ010000435 DATCBJ010000000
VERSION     DATCBJ010000435.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 4913)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4913)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4913
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_301581"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1010
                     /locus_tag="VII50_08820"
     CDS             <1..1010
                     /locus_tag="VII50_08820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009075427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEY5050991.1"
                     /translation="SLLQRPFSFQFYEAVENLRRTLDIGITTIRDAHGADLGVQQAVE
                     RGLIPGPRMQISVTAISQTGGHGDGWMASGNCVELMPTHPGRPSGIVDGPDEMRRKVR
                     ELIRAGADVIKVFTSGGVLSPRDDPRHAHFRDDELAVLVEEATAAGRFVMAHAQGADG
                     IKNAVRSGIRSIEHGIYLDDEGISLMLEHGTWLVPTLSAPAAVLDLVAAGGQVPAAVI
                     EKAHLVIDVHRRAVTRAIEAGVKIAMGTDSGVGPHGQNLRELDLMTQCGMSSAQALVA
                     TTSEAARLLGVDGDRGTIEPGKRADLVVLAGGLEDMTGLADRVRQVWKDGQLVAGTER
                     T"
     gene            1092..2513
                     /locus_tag="VII50_08825"
     CDS             1092..2513
                     /locus_tag="VII50_08825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004030 - aldehyde dehydrogenase [NAD(P)+]
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent succinic semialdehyde
                     dehydrogenase"
                     /protein_id="HEY5050992.1"
                     /translation="MAIASTNPATGEVVKAFDSMTHDEIEVCLERAHRGYEVLRRTSF
                     AQRAAWMRAAADILDVEIDDVARTMTIEMGKTLASAKGEVAKCAKGGRFYADHGEEFL
                     ADEVVDPTTVGAKGALVRYHPIGPVLAVMPWNFPLWQAMRFAAPALMAGNVGLLKHAS
                     NVPQSALFLEDLFRRAGFPEGSFQTLLIGSADVERVISDPRVRAVTLTGSEAAGRAVA
                     AVAGRELKKTVLELGGSDPFVVMPSANIAEAAAIATTARCQNNGQSCIAAKRFIVHTD
                     VYDAFAEAFVAKMSSLRVGDPMVAGTDVGPLATEQGRADIEEQVRDAVAKGASVLCGG
                     ARLDQPGWWYQPTVVADLTPDMAMYAEEVFGPVAGLYRVNSREEALELANSTAFGLGS
                     NAWTNDGPEQEFFMAGLDAGAVFINGMTTSYAELPFGGVRNSGYGRELSSHGMKEFCN
                     AKTVWIGSSAGGAGIPTAGDHSE"
     gene            complement(2519..2740)
                     /locus_tag="VII50_08830"
     CDS             complement(2519..2740)
                     /locus_tag="VII50_08830"
                     /inference="COORDINATES: protein motif:HMM:NF021552.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2277 domain-containing protein"
                     /protein_id="HEY5050993.1"
                     /translation="MCRSIKTLRPPFVDEATSEDVAAAALQYVRKIAGMRKPSAANQQ
                     AFDEAVAEIAAATEHLLGHLQVRQPAPAR"
     gene            complement(2787..3803)
                     /locus_tag="VII50_08835"
     CDS             complement(2787..3803)
                     /locus_tag="VII50_08835"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020657924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016717 - oxidoreductase activity, acting
                     on paired donors, with oxidation of a pair of donors
                     resulting in the reduction of molecular oxygen to two
                     molecules of water [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA desaturase"
                     /protein_id="HEY5050994.1"
                     /translation="MTAQSVDVPIRRGSDYAMLSRIVKDAGLLERRKLYYWLRIALNI
                     VLVAAAGFTLYLANHTWWVLLVAVFFAIVSTQLAFTGHEAGHRQMFRSRQANELAGYL
                     HGNLMIGLSYDWWVDKHNRHHRNPNQVELDPDVGTGAFAFSREEAAKRGRIGRFAARW
                     QSFYFFPLLTLEALNLKVASIRAVAKTGARNRGIEASLLFVHVAGYITLLLLTMSPLQ
                     ALAFLGVHQALFGVYLGCSFAPNHKGMKMLTKDEELDFLRRQVLTSRNVTGGVPVGML
                     LGGLNWQIEHHLFPSMPRPNLRRAVPIVRDFCETHGLPYAEASLYRSYRLAMGHLHDV
                     GASA"
     gene            4006..4188
                     /locus_tag="VII50_08840"
     CDS             4006..4188
                     /locus_tag="VII50_08840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5050995.1"
                     /translation="MRIRSLFNLRRDLVIAIAVPVAGKVMSVAAHQLRERRGPNQLAD
                     RLDQGAALIRRVSRLV"
     gene            4474..4677
                     /locus_tag="VII50_08845"
     CDS             4474..4677
                     /locus_tag="VII50_08845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006243848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HEY5050996.1"
                     /translation="MAQGTVKWFNAEKGFGFISPDGGDADVFVHHSQIQTNGYRSLDE
                     NQRVEFEVTQGPKGPQAEQVRPV"
BASE COUNT          848 a         1545 c         1682 g          838 t
ORIGIN      
        1 tgtcattgct ccaacgtccg ttctcgttcc agttctacga agccgtcgaa aacctcagac
       61 gcacgctcga cattggcatc accacgattc gcgacgcgca cggtgccgac ctgggcgtgc
      121 agcaggcagt cgaacgtggc ctgattcccg gtccccgcat gcagatctcg gtgacagcca
      181 tcagtcaaac tggcggtcac ggcgacggct ggatggcgtc gggcaactgt gtcgagctga
      241 tgccaaccca cccgggccgg ccgtctggaa ttgtcgacgg gcctgacgag atgcgacgca
      301 aggttcgcga gctgatcaga gccggcgccg acgtcatcaa ggtattcacc tcgggtggtg
      361 tgttgtcgcc gcgcgacgac ccgcggcacg cgcatttccg cgatgacgaa ctcgcggtgc
      421 tcgtcgagga ggccaccgcg gctgggcgct tcgtcatggc ccacgcgcaa ggcgccgacg
      481 gcatcaagaa cgcggtgcgc agtggcatcc gatcgatcga gcacgggatc tacctcgacg
      541 acgagggcat ctcgctgatg ctcgaacacg gcacctggct ggtgccgaca ctgtcggctc
      601 ctgcggccgt gctcgatctc gtggccgccg gtggtcaggt ccctgccgcg gtcatcgaga
      661 aggcgcatct ggtgatcgac gtccatcgtc gcgctgtgac ccgtgccatt gaggcggggg
      721 tgaagatcgc gatgggaacc gacagcggcg tcgggccgca tgggcagaac ctgcgagagc
      781 tcgacctcat gacgcagtgc ggaatgtcat ccgcacaggc gctggtcgcg acgacgtcgg
      841 aggcggcgcg gctgctcgga gtcgacggcg accgcggcac gatcgagccg ggcaagcgcg
      901 cagatctggt ggttctggcg ggcggtctcg aggacatgac cggcctcgct gatcgcgtcc
      961 ggcaggtctg gaaggacggt caactggtgg ccggcaccga gcgcacctga gtgcagcgtg
     1021 gcgtcgatgg gccggtagcc tcaaaccggt aacttccaac ccggtaactc cgaagggcgg
     1081 aggccacccc catggcgatc gcttccacga acccggcgac cggcgaggtc gtcaaggcgt
     1141 tcgactcgat gacgcacgac gagatcgagg tgtgcctcga gcgagcgcat cgcggctacg
     1201 aggttctccg tcgcacgtcg tttgcgcaac gcgcggcgtg gatgcgagcc gccgcggaca
     1261 tcctggatgt cgaaatcgac gacgtcgccc gcaccatgac aatcgagatg ggcaagacgc
     1321 tcgcgagcgc gaagggcgag gtcgcgaaat gcgcaaaggg tggccgtttc tacgccgacc
     1381 acggcgaaga gttcctcgct gacgaggtgg tcgatccgac cacggtcggc gccaagggcg
     1441 cgctcgtgcg ctaccacccg attggcccag tgctcgcggt catgccatgg aatttccctt
     1501 tgtggcaagc aatgcggttc gcagcgccgg cgttgatggc gggcaatgtc gggctactga
     1561 agcatgcgag caatgtgccg cagtctgcgc tgttcctcga agatctgttc cggcgcgcag
     1621 ggttccccga aggctcgttc cagacgttgc tgatcggctc agccgacgtc gagcgcgtta
     1681 tctccgaccc cagagtcaga gcggtcacgc tgaccggcag cgaagctgcc gggcgcgcgg
     1741 tcgctgcggt tgccggccgc gagttgaaga agacggtgct cgagcttggc ggcagcgacc
     1801 cgttcgtcgt gatgccgtcg gcgaacatcg cggaggcggc ggcgatcgcc acgaccgcac
     1861 gctgccagaa caacggtcag tcatgcatcg cggccaagcg gttcatcgtg cataccgacg
     1921 tctacgacgc gttcgccgag gcgtttgtgg caaagatgtc gtcgttgcgg gtgggcgacc
     1981 cgatggtcgc cggcaccgac gttggcccgc tggcgaccga gcagggtcgg gcagacatcg
     2041 aggaacaggt gcgcgatgcg gtcgcgaagg gcgcgtcggt cctgtgtggc ggcgcccgtc
     2101 tcgaccagcc gggctggtgg taccagccga ccgtggtcgc cgacctgacg ccagatatgg
     2161 cgatgtatgc cgaagaggta ttcggcccgg tcgctggtct ctaccgggtg aactcacgcg
     2221 aagaggcgct cgagctcgcc aacagcaccg cgttcggtct tggatccaat gcctggacca
     2281 acgacgggcc cgagcaggag ttcttcatgg ccggtcttga tgccggcgcc gtattcatca
     2341 acggcatgac gacgtcgtac gccgagctac cgttcggtgg cgtccgtaac tccggctacg
     2401 gccgcgagct gtcgagccac ggcatgaagg aattctgcaa cgcgaagacg gtctggatcg
     2461 gcagcagcgc cggtggcgcc ggcatcccga ccgccggcga ccacagcgag taagcggcct
     2521 agcgcgcggg cgcgggctgc cgtacctgca agtgcccgag cagatgctcg gtcgctgcgg
     2581 cgatctccgc taccgcttca tcgaacgctt gctggtttgc cgccgacggc ttgcgcatgc
     2641 cggcgatctt tcgcacgtat tgcagcgcag cggcagcgac atcctctgag gtcgcctcgt
     2701 ctacgaacgg tgggcgcagg gtcttgatgc ttcgacacat ggcgcccagc ctacgacgcg
     2761 accgccgtcc ggcgtccgat caagtcttag gcgctggctc cgacgtcgtg caggtggccc
     2821 atggccagcc ggtaggagcg gtacagcgat gcctcggcgt agggaaggcc gtgggtctcg
     2881 cagaagtccc gcacgatcgg caccgcgcgg cgcaagttcg gccgcggcat cgaggggaac
     2941 aggtggtgct cgatctgcca gttcaggccg ccgagcaaca tgccgaccgg cacgccgccg
     3001 gtgacattgc gcgaggtcag gacctgcctg cgcaggaaat ccagctcctc gtccttcgtc
     3061 agcatcttca tgcctttgtg gttcggcgcg aatgagcagc cgagatagac accgaacaaa
     3121 gcttgatgga cgccgaggaa agccagcgct tgtagaggcg acatcgtcag cagcaacagc
     3181 gtgatgtatc cggcgacgtg tacgaagagc aagctcgctt cgatgccgcg gttgcgcgcg
     3241 ccggtcttcg ccaccgctcg aatgctggcg accttgaggt tcagcgcttc gagagtgagc
     3301 aacgggaaga agtagaacga ctgccagcgg gccgcgaacc ggccgatgcg gccgcgcttt
     3361 gctgcctctt cgcggctgaa cgcgaaggca ccggtgccga cgtcgggatc gagttcgacc
     3421 tggttgggat tgcggtggtg ccggttgtgc ttgtcgaccc accagtcata gctcaggccg
     3481 atcatcaggt tgccgtgcaa gtagccagcc agctcgttcg cctggcggct gcgaaacatc
     3541 tgtcgatggc cagcctcgtg gccggtgaag gcgagttggg tcgaaacgat ggcaaagaac
     3601 acggcgacca gcagcaccca ccaggtgtgg tttgctaggt agagcgtgaa gcccgcagcg
     3661 gccaccagca cgatattgag cgcgatccgc aaccagtagt acagcttgcg acgctcgagc
     3721 agcccggcgt ccttcacgat ccgcgacagc atggcgtagt cgctgcctcg acggatggga
     3781 acgtcaacgg actgagcagt cacggcgatt ccattccggg cgcttgcgcc gatgtcttcg
     3841 ccacggggtc tggatggcgg gtggtgtcga ggagccactg ctccgcgtcc gaagtgtctc
     3901 acggcggggg gcgcccgcgt ccagagtcca tgggtcgtgg cgtttgagtc gctggaccgt
     3961 ggcgttcgcc gcatagaatg ctcgccccgt gggcaggagg ccagcgtgag gatccgaagc
     4021 ctgttcaacc ttcggcgcga cctcgtcatc gcgatcgccg ttcccgtcgc cgggaaggtg
     4081 atgtcggtcg ccgcccacca gttgcgcgag cgtcgcgggc ccaatcagct cgcggatcgc
     4141 cttgaccagg gcgctgcgct gatccgacgg gtcagcaggc tggtctgagc gcggccggcc
     4201 cagcgcatcc cccaactgtg cgcatcccca actgagtgca ccccaaccga cggcagcaat
     4261 gccgtggtag ggtcgccgta ggttgcagtt gcagtactcc tcgaacgttc atgacgcccg
     4321 cggattctgg cagcgggcgt cttttgtttg agttgagtcg gtagcgcttc gcgtttgcgc
     4381 ggatttgcac ctcgttaacc caagcaaacc cggcgtcgca gcccgagccg ccgacgtggc
     4441 gtctcgtagc acacctaacg aggagtaccc cagatggcgc aaggcaccgt gaagtggttc
     4501 aacgcggaaa agggcttcgg tttcatcagc cccgacggcg gcgacgctga cgtgttcgtt
     4561 caccactcgc agatccagac gaacggctac cgcagcctcg acgagaacca gcgtgtcgag
     4621 tttgaggtca cgcagggccc gaagggcccg caggctgagc aggttcgccc ggtctaagtc
     4681 ttggaacaaa cagcagcggc tgtgctcggt ggaaacgccg ggcacagccg ctttctgttg
     4741 ggagcgttga tgcggggcgc gcgttcttgc acgcgctgag caccaatggt cgcgcgcgag
     4801 ggccggtggc gttggtttag atgtctggga cgccgtgcgg gtggctgaac agcgccgccg
     4861 acgacgattc gccgaactcc tcccgcgcca cgccggcttc gaccatttcg cgt
//
LOCUS       DATCBJ010000436         3294 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_447119, whole genome shotgun sequence.
ACCESSION   DATCBJ010000436 DATCBJ010000000
VERSION     DATCBJ010000436.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 3294)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3294)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3294
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_447119"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..332)
                     /locus_tag="VII50_08850"
     CDS             complement(<1..332)
                     /locus_tag="VII50_08850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5050997.1"
                     /translation="MQPNSVPVPEVFLITDIADRRTRSCRIARRSGCAAVALVPNPYS
                     LVLHEPRVAMDDDARREFGTSRREFAARGSPGRATWFAQVQSISQVAKSIEPTDRPAE
                     QRSAAQHE"
     gene            complement(419..1615)
                     /locus_tag="VII50_08855"
     CDS             complement(419..1615)
                     /locus_tag="VII50_08855"
                     /inference="COORDINATES: protein motif:HMM:NF012245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HEY5050998.1"
                     /translation="MSEAMAANAENSAEQASSDAAAADQMSNSIAIVAHGVDQLATTI
                     RDISEHAAEASTTATEASVEATGARQAVAELAEASLQVREMVQFITGVAGQTHLLALN
                     ARIEAARAGDAGRSFAVVAGEVRQLADATSRATTSANRSVEDIQSGSALAAEAMATIV
                     DTIRRVTSNQLSIAAAVEEQTMTTHDMGLRAREIAAGVHQVAAGVALLAGEARVMAYS
                     GGLAWAAATELSGAGRDLEHALSGLSLPPLAPSEDDTVTNTAATPDGRGRTIVLNSAF
                     GARQNELHYAGRWLHSRGNLDGDTANSYSGFPGDTVTLRFRGSQIRLYAVTGPNHGIA
                     TVSVDGGPEAECDQYSEQRTTHVLLWAAECLPATDHLLTYTVSGAKNTKSRYVWVTLE
                     RLEISE"
     gene            complement(1858..2052)
                     /locus_tag="VII50_08860"
     CDS             complement(1858..2052)
                     /locus_tag="VII50_08860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5050999.1"
                     /translation="MPTVVMRRPAPAAVRRVDLLLAMLAVAGSATATWRIEGVSDRTM
                     TMVRSAGFFAAFGSPCSVRP"
     gene            2343..>3294
                     /locus_tag="VII50_08865"
     CDS             2343..>3294
                     /locus_tag="VII50_08865"
                     /inference="COORDINATES: protein motif:HMM:NF013697.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY5051000.1"
                     /translation="MSLGFARGRKRMLVVAPTVVALTLLAACSSSKKSTAASSTSASP
                     ATSSASAPVSAAGAASSGAASPAVSSAPAVSSAPAASGSGAAPAPQAKTCADYAQYTS
                     AATKGKTVTMFGSILPPESDSLNKSWAQFESCTGIKISYEGSNDFESQLPVRVQGGTA
                     PNLAIIPQPGLLQQMVALGAVKAPPAGVVANEDKYWNAAWKSYGSVNGTFYAAPQSAN
                     MKSLVWYSPSAMSKAGYTIPTTWDQLAALSDKIAATGTKPWCGGIGSGTATGWPATDW
                     LEETVLSSQGGQVYDDWISHKIKFSDPQIQTAMTDVANWMQ"
BASE COUNT          562 a         1067 c         1062 g          603 t
ORIGIN      
        1 tcttcgtgtt gtgcggctga cctttgctcg gccggtcggt cggtcggttc gatgctcttg
       61 gcaacttgcg agatcgactg aacctgggca aaccaggtcg cccgccccgg gctacctctt
      121 gcggcgaact cgcgcctcga cgtcccaaac tcacggcgcg cgtcgtcgtc catcgcgact
      181 ctcggttcgt ggaggaccag cgagtagggg ttcggaacca acgcaacagc tgcgcacccg
      241 ctgcgtctgg ctatccgaca acttcgggtg cgccggtcgg cgatgtcggt gatgagaaac
      301 acctcgggga cgggcacgga gttcggctgc acgacgccct cgatgacgtg cgcgcgcgcg
      361 ttcagcactt gcgcgacaca gcggtggcta cggcactgcc gtcggatcct tcagcccgtt
      421 actcggatat ctcgaggcgt tcgagtgtca cccacacata gcgagacttg gtgtttttcg
      481 cgccactgac cgtgtatgtg agcaaatgat cggtggcggg taagcattcc gcagcccaca
      541 gcaggacgtg cgtcgtacgc tgctcgctgt attgatcgca ctcagcctcg ggaccgccgt
      601 cgactgacac ggtcgcgatg ccgtggttgg gaccggtcac cgcgtatagg cgtatctggc
      661 tgccgcgaaa gcgtagggtg acggtgtcgc ctgggaaacc gctgtaggag tttgcggtgt
      721 cgccgtcgag gtttccgcgc gagtggagcc accggcccgc gtagtgaagc tcgttttggc
      781 gagcgccgaa agcactgttc agaacaatgg tgcgtcctcg accgtccggg gtagccgccg
      841 tgttcgtgac cgtgtcatcc tcagacggtg ccagcggcgg caagctgagt ccacttaatg
      901 cgtgctccaa gtcgcgtccc gcgcccgaga gttccgtcgc agcggcccac gccagaccac
      961 ccgagtaggc catgacgcgg gcttcgcctg cgaggagcgc aacgcctgca gcaacttggt
     1021 gaacgcccgc ggctatctcg cgcgcgcgaa gacccatgtc gtgtgtcgtc attgtctgct
     1081 cttcgacggc ggcagcaatc gagagctgat tcgacgttac ccggcgaatg gtgtcaacga
     1141 tggtggccat cgcttcggcg gccagcgcag agcccgactg gatatcttcc acggagcggt
     1201 tcgcggaggt ggttgcccgt gacgttgcgt ccgccaactg ccgtacctca ccagcaacaa
     1261 cggcgaagct ccggcccgcg tcaccggcgc gcgccgcttc aatccttgca tttagcgcta
     1321 agaggtgggt ttgccccgcc acgcccgtaa tgaactggac catctcgcgt acctgaaggg
     1381 atgcttcggc cagctcggca actgcttgcc gtgcgccagt cgcctcgacg gacgcttcgg
     1441 tggccgttgt cgatgcttcg gctgcgtgct cggagatgtc gcgaattgtg gttgcgagct
     1501 ggtcaacacc gtgggcaacg atggcgatgc tgttggacat ctggtcagcc gccgcggcgt
     1561 cgctagaagc ctgctcggcg gagttctcgg cgttagccgc catcgcctcg ctcaccgcga
     1621 ggagctcctt cgaccgcgcg gcgagtgcga atgcggccgt ttgagaagtt cggaagatag
     1681 gttgtagcgc tgcacctagg ttccttgcgg cgtgaaacac ccccgcgatt tcgggatccg
     1741 cagacgagct ggggggctgg agttcggcga gcgcgtcagt tcgtcccagg agcgactctt
     1801 caagtgcgcg cctgaggatc ctcagctccc ggtcccgcgc gcgccgtcgc gcggacctca
     1861 tggccgcacc gagcatggcg agccgaacgc cgcgaagaat cctgccgacc gcaccatggt
     1921 catggtgcgg tccgagacac cctcgattcg ccacgttgcc gtagcagaac ctgccacagc
     1981 caacattgcc aatagcaggt caacccgccg cacggcggct ggcgccggac ggcgcataac
     2041 gacggttggc atcgttcctc ctgttcaacg cagtcgtggc cggccggtgc gacggtgtgt
     2101 ctgagcgact atcgccagtt tcccgtgtcg cccgacgcat cgcggatgtc ctggcagttt
     2161 cggcattcgg accacagcgg ggccgcctgg agactccgtc cgcaagcgat gccaaaccgc
     2221 aactgtttgc acgggttttc acagagcccg gccttgcttt gggggattcg gagcattcac
     2281 gatgtcaacg tttgcagccg cgcggctcct gccgcaccgg accagtcaca agaggagatc
     2341 tcatgagttt gggttttgcg cgaggcagga aacgaatgct cgtcgtggcg ccgacggtgg
     2401 tcgctctcac gctgctcgcc gcgtgttcga gtagcaagaa gtcgacggct gcctcgtcca
     2461 cgtccgccag ccccgccacc tcatccgcgt ctgctccggt gtcggccgcg ggggcagcca
     2521 gcagcggagc tgcttctccc gcagtcagct ccgcgccggc agtcagctcg gcgccagcag
     2581 cctcaggcag cggagcagcg cctgcgcctc aggccaagac ctgcgccgac tacgcgcagt
     2641 acacaagtgc cgccaccaag ggcaagacgg tgacgatgtt cggctcgatc ctgccgccgg
     2701 agtcagactc gctcaacaag tcatgggcgc agttcgaaag ctgcaccggc atcaagatct
     2761 cctacgaagg ctccaacgac ttcgagtcgc agctgcccgt gcgtgttcag ggtggtaccg
     2821 cacctaacct ggcgatcatc ccgcagcctg gtttgctgca gcagatggtc gcgctcggcg
     2881 ccgtcaaggc tccgccggcc ggtgtcgtcg cgaacgagga caagtactgg aacgcggcct
     2941 ggaagtcgta cggctctgtc aacggcacgt tctacgcagc gcctcagagc gcgaacatga
     3001 agtcgctcgt gtggtactcg ccgtcggcta tgtccaaggc gggctacacg atcccgacga
     3061 cgtgggacca gctcgcagca ctgtctgaca agatcgcggc caccggcacc aagccgtggt
     3121 gcggcggtat cggatcaggt acggcgaccg gctggccggc caccgactgg ctcgaagaga
     3181 ccgtgttgag ctcgcaaggc ggccaggtct acgacgactg gatcagccac aagatcaagt
     3241 tctccgaccc gcagattcag accgcgatga ccgatgtcgc caactggatg caga
//
LOCUS       DATCBJ010000437         2404 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_246060, whole genome shotgun sequence.
ACCESSION   DATCBJ010000437 DATCBJ010000000
VERSION     DATCBJ010000437.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2404)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2404)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2404
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_246060"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1..2314)
                     /gene="rpoC"
                     /locus_tag="VII50_08870"
                     /pseudo
     CDS             complement(1..2314)
                     /gene="rpoC"
                     /locus_tag="VII50_08870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020417557.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
BASE COUNT          403 a          791 c          787 g          423 t
ORIGIN      
        1 cgacccggat gttgcggtag cggggcaaac ccgtaccggc cgggatgagc ttgccgatga
       61 tgacgttctc cttgaggccc aacagcgggt cggacttggc gtggatcgcc gcatccgtca
      121 gcacccgggt ggtctcctgg aacgacgccg ccgacagcca cgactcagtt gcgagcgaag
      181 ccttggtgat gcccatcaag accggtcgag cggacgccgg agcgccaccc tcggcgacga
      241 cccgacggtt gccttcctcg aaccgaggac gctcgaccag ctcgccgggc aggaagtcgg
      301 tgtcacccga ctccaggacg ttcacccgct tcagcatctg ccgcacgatg atctcgatgt
      361 gcttgtcgtg gatcggcaca ccctggctgc ggtagacctc ctggacctcc tggaccaagt
      421 ggacctgcac cgcgcgtggg ccgaggatcc gcagcacctc gtgcgggttc accgtgccct
      481 cgatcaactg ctcgccgacc tcgacgtggt cgccttcggc gacccgcagc cgcgaccgct
      541 tcgacaccgg gtaggcgatc tcctcggctc cgtcatcagg aacgatgacg atcttgcgag
      601 ccttgtcggt gtcctcgatc ttgacccggc cggtgacctc actgatcggg gcaacaccct
      661 tgggggtgcg ggcctcgaac agctcgacga cgcgcggcag gccgtgggtg atgtcttcac
      721 ccgcgacacc gccagtgtgg aaggtacgca tcgtgagctg ggtgccaggc tcgccgatcg
      781 attgggcggc gatgatgccg accgcctcgc cgacgtcgac ggtcttgccg gtcgccaacg
      841 agcggccgta gcaggccgcg caagtgccga ccttcgactc acacgtcaac accgagcgaa
      901 ccttcaactc ctgcacgccg tgctcgacga gcgcgccgat gctgaggtca ccaaggtcac
      961 cgcgtgcttc gccgaccaac ttgccgtcgt gaatgatgtc ctcggcgatg gtgcgagcaa
     1021 agacgctggt ctcgaccgtg gccgggcgaa tcagcacccc gtcacgacca ggcgcggcga
     1081 tgcgcatcat gatgccgcgc tcggtaccgc agtcctcttc gcggatgatg acgtcctgcg
     1141 atacgtccac gaggcgccgg gtcaggtaac ccgagtctgc ggtacgcagc gccgtgtcag
     1201 ccagaccctt acgggcaccg tgggtggaga tgaagtactc cagcacggac aggccctcgc
     1261 ggaagttcgc cttgatcggc cgcgggatga tctcgccctt ggggttggcg acgagaccac
     1321 gcatgccagc gatctgccgg acctgcatca tgttgccgcg ggccccggag ttcaccatca
     1381 tccagaccgg gttgtccttg gggaagttct cttccatctc gcgggcaacg tcggcggtgg
     1441 cctgggtcca gatctcgatg agctcctggc gacgctcgtc gtcggtgatc agcccacgct
     1501 cgaactgctt ttggaccttg tccgccttgg cctcgtactt ctccaggatc tcctgcttgt
     1561 tcggtggggt gacgacgtcg gagatcgaga tcgtgacgcc ggcgcgggtg gcccagtgga
     1621 agccggccgc cttcatcgcg tcgagcgtcg cagcgacgac caccttcggg taccgctcgg
     1681 cgaggtcgtt gacgatcgtc gagaggttgc gcttggagac ctcgaagttg acgaaccggt
     1741 agtcctcggg cagcgcgtcg ttgaacagcg cgcggccaag cgtggtctcg acgatcagcg
     1801 gctgaccagg ctcccagccc tcgggcgccg tccagccgtc cttgccgttg tcgacggcag
     1861 ggagatcctt gagccggatc ttgatcttgg cctgcaggtg caggtcaccg agatcgcgag
     1921 ccatgacggc ttcagcggtc gaggagaaca cgcggccctc acccaacaca ccatcgcgcg
     1981 cagtcgtcag gaagtagatg ccaagcacca tgtcctggct cggcattgtc accgggcggc
     2041 cgtctgcggg cttgaggatg ttgttcgagg acagcatcag gatgcgggcc tcggcttggg
     2101 cctcagccga cagcggcaag tgcacggcca tctggtcgcc gtcgaagtcg gcgttgaacg
     2161 ccgcgcagac gagcgggtgg atctggatgg ccttgccctc aaccagttgc ggctcgaacg
     2221 cctggatgcc aaggcggtgc agggttggtg cacggttcag cagcaccggg tgctcgcgga
     2281 tcacctcctc gaggatgtcc cagacctgcg gctgctcgag ctgaacgcgc ccgagatcat
     2341 catcaagaac gaaaagcgta tgctgcaaga agcggtcgac gcgctgatcg acaacggccg
     2401 ccgc
//
LOCUS       DATCBJ010000438         2328 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_420256, whole genome shotgun sequence.
ACCESSION   DATCBJ010000438 DATCBJ010000000
VERSION     DATCBJ010000438.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2328)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2328)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2328
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_420256"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            101..1381
                     /gene="eno"
                     /locus_tag="VII50_08875"
     CDS             101..1381
                     /gene="eno"
                     /locus_tag="VII50_08875"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018225678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HEY5051001.1"
                     /translation="MAAIEALGAREILDSRGNPTIEVEALLDDGTIARAGVPSGASTG
                     AFEAVELRDGGQRYGGKGVEKAVQAVIDDIAPEVIGFEASEQRLLDAKLIELDGTPNK
                     SKLGANAILGVSLAVARAAAESAELPLFRYLGGPNAHLLPVPMMNIVNGGSHADSNVD
                     IQEFMIAPIGAGTFAEAFRWGAETYHALKAVLKARGLSTGLGDEGGFAPNLDSNREAL
                     DLILEAIAKAGFTPGRDIALALDVAATEFHKDGAYAFEGGSKSASDLISYYGDIVANY
                     PLVSLEDPLDEEDWEGWKAITDALGSKVQIVGDDLFVTNPIRIARGIESATANALLVK
                     LNQIGTLTETLDAVDMAHRAGYRTMVSHRSGETEDTTIADFVVATNAGQIKSGAPARG
                     ERIAKYNQLMRIEEELDDAARYAGALAFPRFVVG"
     gene            1480..2046
                     /locus_tag="VII50_08880"
     CDS             1480..2046
                     /locus_tag="VII50_08880"
                     /inference="COORDINATES: protein motif:HMM:NF016839.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HEY5051002.1"
                     /translation="MADGELSVDGPRHGKPTFTGRAAVLAVVVAALALALAAPARELL
                     SQRSQISSLNSSIATQEQQVAALQKAQKQWANSTYVEQQARERLHYVFPGQVPYVTLS
                     PTPVASASAAAAQALASQPWYGRLWSSVQVAANVPVTPKPTPSPTLAPALVSPPAASA
                     PVITPVVTASPSAAPTAASTGAAGFRGP"
     gene            2043..>2328
                     /locus_tag="VII50_08885"
     CDS             2043..>2328
                     /locus_tag="VII50_08885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF501 domain-containing protein"
                     /protein_id="HEY5051003.1"
                     /translation="MTSPAVSRAGEPTADDLAVVERQLGRTPRAVRSVVHRCECGLPD
                     VIESAPRLPDGSPFPTFYYLTCPRAAGAVGTLEAAGLMRQMSARLQIDDEL"
BASE COUNT          390 a          759 c          782 g          397 t
ORIGIN      
        1 tgcgcgccgc cgcccgccgc taccgcgggt tggtgcaggc cgctgaagga gcgggccggt
       61 aaactgacgg cccgccttcc cgatgctcag gagtaccact gtggccgcca tagaagccct
      121 cggggctcgc gaaatcctcg attctcgagg caacccgacc atcgaagtcg aggcccttct
      181 cgacgacggc acgattgccc gcgcaggcgt tccgagtgga gcgtcgaccg gcgccttcga
      241 ggccgtcgaa ctgcgtgacg gcggccagcg ctacggcggc aagggtgtcg agaaggccgt
      301 gcaggccgtc atcgacgaca tcgctcccga ggtcatcggt ttcgaggcga gtgagcagcg
      361 gctgctcgac gcgaagttga tcgaactcga cggcacgccg aacaagtcca agctcggtgc
      421 caacgcgatt ctcggtgtgt cacttgcggt ggccagggcc gccgccgaat ccgctgagtt
      481 gccgttgttc cgctacctcg gcggccccaa cgcacacctg ctgccggtgc cgatgatgaa
      541 catcgtcaac ggcggatcgc acgccgactc caacgtcgac atccaagaat tcatgatcgc
      601 cccaatcggt gccggcacgt tcgccgaggc atttcgctgg ggcgccgaga cctaccacgc
      661 gttgaaggca gtgctgaaag cgcgaggtct ttcgaccgga ctcggcgacg agggcgggtt
      721 cgcaccgaac ctcgacagca accgtgaagc tctcgacctg atcctcgagg ccatcgcgaa
      781 ggccggcttc actcctggcc gcgacattgc actcgcactc gacgttgccg ccaccgagtt
      841 tcacaaggat ggcgcgtacg cattcgaggg cggcagcaaa tcggcgtccg atctgatttc
      901 gtactacggc gacatcgtcg cgaactaccc gctggtgtcg ctcgaggacc cgctcgacga
      961 agaggactgg gagggctgga aggcgatcac cgacgcgctg ggctcgaagg ttcagatcgt
     1021 cggcgacgac ttgtttgtca ccaacccgat tcgcatcgca cgggggattg agtcggcaac
     1081 cgcgaacgcg ctgctggtga agctgaacca gatcggcacg ttgaccgaga ccctcgacgc
     1141 cgttgacatg gcgcatcgtg cgggctaccg aacgatggtc tcgcaccgat ccggtgagac
     1201 cgaggacacc acgattgccg acttcgtggt cgcgacgaat gccggtcaga tcaagagcgg
     1261 tgcgccggcg cgcggcgaac gcattgcgaa gtacaaccag ctcatgcgca tcgaggaaga
     1321 gctcgacgac gcggcgcgct acgcaggcgc gttggcgttc ccccgattcg tggtcggctg
     1381 acgttgcgcc gccgacgccc ggcagacgac ggcgctgcgc cggatgtcgg tgatgcagct
     1441 cgcgttggcg cgactggcgt tggtgcggcg gccggtgaaa tggcggacgg cgagcttagc
     1501 gttgatgggc cgcgtcacgg caagccgacg tttaccggac gagctgccgt gcttgccgtc
     1561 gtggttgcgg cgttggcgct tgcgctcgcc gctccggcac gtgagttgct cagtcagcgc
     1621 tcacaaatct cttcgctgaa cagcagcatt gccactcagg agcagcaggt cgcggcactg
     1681 cagaaagccc agaagcaatg ggcaaactcg acgtacgtcg agcagcaggc gcgcgaacgg
     1741 ctgcactacg tgttcccggg gcaagtgccc tacgtgacgt tgtcgccgac acccgttgcg
     1801 tccgcgtcgg ccgcggctgc gcaggcgctc gcatcgcagc cgtggtacgg ccgcttgtgg
     1861 tcgtcggtgc aagtagcagc gaatgtgcca gtgacgccaa agccgacgcc gagtccgacg
     1921 ttggccccgg ccctcgtttc gccgcccgcc gcgtcggcac ctgtgattac ccccgtcgtt
     1981 acggcgagtc cgagtgccgc tccgactgct gcatcgacgg gtgcggccgg gttccgcggt
     2041 ccgtgaccag ccctgcggtg tcgcgcgccg gtgaaccgac cgccgacgac ctcgctgtcg
     2101 tcgaacggca actcggtcgg acgccgcgtg ctgtgcgcag tgtcgttcat cgatgtgagt
     2161 gcgggctgcc cgacgttatt gaatcggcgc ctcggctgcc tgatgggtca ccgtttccga
     2221 cgttctacta cttgacgtgt ccgcgcgcgg cgggtgcggt cggcaccctc gaggcggccg
     2281 gtctgatgcg tcagatgtca gctcggctgc agattgacga cgagctcg
//
LOCUS       DATCBJ010000439         2097 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_40700, whole genome shotgun sequence.
ACCESSION   DATCBJ010000439 DATCBJ010000000
VERSION     DATCBJ010000439.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2097)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2097)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2097
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_40700"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(19..543)
                     /locus_tag="VII50_08890"
     CDS             complement(19..543)
                     /locus_tag="VII50_08890"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009085526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEY5051004.1"
                     /translation="MADETFATLHTTKGDIEIRLFADHAPKTVANFVELAEGTKEWKD
                     PRSGSKTTSKLYDGTVFHRVIDGFMIQGGDPLGSGRGGPGYQFADEFHPELAFNRPYL
                     LAMANAGPNTNGSQFFITVGPTPHLNRKHTIFGEVTKGSDVVDAIATAPTASGDRPRM
                     DIVINSVTIERRPA"
     gene            737..1351
                     /locus_tag="VII50_08895"
     CDS             737..1351
                     /locus_tag="VII50_08895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5051005.1"
                     /translation="MPVTTAKMTDIAAARIGAARQLVGTRIVPVAVPAGRKAAKQTRQ
                     LVGERIMPAMGPACSKAAKRTRQLLDQRIAPAFGPFGRKAAKQTRQFVGERIMPAVGT
                     ALVVTAPIRGEALRRAGLAAAALRTGEGVVIKKRRRWPTALMCLGLGGVLGATAAWLA
                     QAGKPVQLTPYPLETDGMDDTELHAMNGSHPLDLTDDSPAHHHA"
     gene            complement(1407..>2097)
                     /locus_tag="VII50_08900"
     CDS             complement(1407..>2097)
                     /locus_tag="VII50_08900"
                     /inference="COORDINATES: protein motif:HMM:NF014862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Clp protease N-terminal domain-containing
                     protein"
                     /protein_id="HEY5051006.1"
                     /translation="LSLHHDYIGTEHLLLGLIREREGLGARLLIESGVDLLSLRMRVL
                     DLVPPAAAPETRRRPFWARSRHDLPARAEASDVLRTTTATDTSLDEARRLAGDGAVGS
                     HHLLLATLSDSTSAAAKALGSLGIDLDQARSALRDVDVAGTTDELPEEAGRRQLVMRV
                     TGSALVMETTDPTLVAAAQAILVSSGGHEIRGDDARSRGFAEIWRAWQASLQATAERA
                     DEASAAGPATD"
BASE COUNT          339 a          728 c          669 g          361 t
ORIGIN      
        1 ggtctgcggt cacctgcgtt aggccggacg gcgttcgatg gtgacggagt tgatcacgat
       61 gtccatacgc ggccggtcgc ccgacgcggt tggcgccgtc gcgatcgcat cgacgacgtc
      121 gctgcccttc gttacctcac cgaagatcgt gtgcttccgg ttcaagtgcg gggtcggccc
      181 aacggtgatg aagaactgcg atccgttggt gttcggtccg gcgttggcca tcgcgagcag
      241 gtacggccgg ttgaaggcga gctcagggtg gaattcgtcg gcgaactggt agcccgggcc
      301 gccgcggcca ctgccgagcg ggtcaccgcc ttggatcatg aacccgtcga tgacccggtg
      361 gaagaccgtg ccgtcgtaca gcttgctcgt ggtcttcgag ccgctcctgg ggtccttcca
      421 ttccttggtg ccctcagcaa gctcgacgaa gttcgccacc gtcttgggag cgtgatcggc
      481 aaagagccga atctcgatgt cgcccttcgt cgtgtgcaaa gtcgcgaaag tctcgtcggc
      541 cactgggtga ttcctcccgg tcggtgcgcg gatacgtcat cttcccacgc gacaggggac
      601 accgtcgcgc cgtaccgtgg acacgaaccc cagacgggct tggttaccgg ccgtctgggg
      661 cgggaatggc aaactgagca ccaactgcac gactcgcgct cccagcttgt tcggtgccgc
      721 tcctacggag gttcgcatgc ccgtcaccac cgcgaagatg accgatattg ccgccgcacg
      781 cattggcgcg gcacgccagc tcgtcggcac ccgaattgtt cccgttgccg tgcctgccgg
      841 tcgcaaggca gctaaacaaa cccgccaact cgtcggcgaa cgcatcatgc cggccatggg
      901 accggcatgt tcgaaggcag ctaagcggac ccgccagctc ctcgaccaga ggatcgcgcc
      961 ggctttcggc ccgttcggtc gcaaggcagc taagcaaacc cgccagttcg tcggcgagcg
     1021 catcatgccg gctgtcggca ctgcccttgt tgtcaccgca cccatccgag gcgaagctct
     1081 tcggcgcgca ggcctggccg ccgccgcact tcgcacaggc gaaggcgtcg tcatcaagaa
     1141 gcgtcggcgc tggccgaccg cgctcatgtg cctcggcctc ggtggcgtgc tcggtgcgac
     1201 agctgcctgg ctcgcgcagg ctggcaagcc ggttcagctc accccgtacc cgctcgagac
     1261 cgacggcatg gacgacaccg agctccacgc aatgaacggc tcacacccgc tcgatctgac
     1321 cgacgattca cccgcgcacc accacgcctg acccgcgtcg ccgtagtttc cgagccagcc
     1381 tggcccggta ccagcctgac catgtgtcaa tctgttgccg ggccggctgc gctcgcctcg
     1441 tccgcccgct cagcggttgc ctgcagactt gcctgccacg cccgccaaat ctcggcaaac
     1501 ccccgactgc gcgcgtcatc gccgcggatt tcgtgaccac ccgacgacac caaaattgcc
     1561 tgcgccgccg cgacaagcgt gggatcggtc gtctccatca ccagggccga gccggtgacg
     1621 cgcatcacca gctgtcggcg tccggcttct tccggaagct cgtcggtggt accggcaaca
     1681 tcgacgtccc gcagtgccga ccgggcctgg tcgaggtcga tgccaagact gcccagggcc
     1741 ttcgcggccg cgctggtcga atcactcagg gtcgctagca gcaaatgatg cgagcccacg
     1801 gcgccgtcgc cggccaaccg ccgtgcttcg tcaaggctcg tatccgtcgc ggtggtcgtc
     1861 cgcaggacat cactagcttc ggcacgcgcg gggaggtcgt gtcgggatcg agcccaaaac
     1921 ggccggcggc gggtctcggg cgcggctgcg ggtggcacga ggtcgagcac cctcatccgc
     1981 agactgagca ggtcgactcc tgactcgata agcagccggg caccgagccc ttcgcgttcg
     2041 cgaatgagcc cgaggagcag gtgctcggtg ccgatgtagt cgtggtgcag gctcaac
//
LOCUS       DATCBJ010000440         3089 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_124417, whole genome shotgun sequence.
ACCESSION   DATCBJ010000440 DATCBJ010000000
VERSION     DATCBJ010000440.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 3089)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3089)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3089
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_124417"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            695..838
                     /locus_tag="VII50_08905"
     CDS             695..838
                     /locus_tag="VII50_08905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5051007.1"
                     /translation="MSATVVLYVRRLDVMRAFYQERFGLKITDAPTPTASSNRTTGRF
                     DSS"
     gene            835..1083
                     /locus_tag="VII50_08910"
     CDS             835..1083
                     /locus_tag="VII50_08910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5051008.1"
                     /translation="MTRHAVTAAQIEVPDPPTRRDLSPIKLGFRVRQIEDLRPVFESL
                     GGRLDAGTAAWEFYGFARLDGVDPEGNVVQLLQPLTRS"
     gene            complement(1312..1743)
                     /locus_tag="VII50_08915"
     CDS             complement(1312..1743)
                     /locus_tag="VII50_08915"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HEY5051009.1"
                     /translation="MLDAYAVVSFLRDEPAADEVASLMRSDDVSLTAVGLGEVVDHLI
                     RLAGVDEDDVFLDLAELGLNDALPIDAQAAGLAGRLRARYYHRTRCAVSMADCLAAAA
                     AHERAVPLATSDPHLLDLCHAESIDTFVLPSTNGSRWSPSK"
     gene            complement(2107..2619)
                     /locus_tag="VII50_08920"
     CDS             complement(2107..2619)
                     /locus_tag="VII50_08920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5051010.1"
                     /translation="MSTPPSVVVGPACRASQLSADLEDFEEAGDSNYGWMIIRDMSGA
                     ACQLTGTIGIVGLDATGTIDTITDHAVVAGGLLLSANAATVPNGAMPLSGERVAFMVP
                     GSSDSITNGARVGYDCPASDEVVPVTFRLDINGTGTITTSNGQPSGSLSHLRICQGQL
                     DAGGPVIGQR"
BASE COUNT          582 a          994 c          982 g          531 t
ORIGIN      
        1 ggcgtccact cagagatacg cccgacggct ggcgcagcaa gtgccgcttc gaatgctccc
       61 atcgccgaac gtagcgattg tcgccgtagg cgcacagcgt cgagatcggg ggcggtaggg
      121 atgtgctggg gacccatcgc agcctcctgc ggcgatctcc agactcccat agccgtcagg
      181 tgaaaaccag gctttgatgg cctctgcgcg agatgctggc ctgagcgagc cgcttcgata
      241 gcaaccggtt gggtgcccgt cgccggatta ctgacacgca aaacattcac ctacgtcaca
      301 ccgggagcgt ttcgcacgcc agcgggcgcg acgggctaca gcaagaccag ccagaggtct
      361 gcaagacaac agcaacggat tcccggctgc gatggcgcgc ggcgtgacgg gtcgtttcga
      421 ggcatcccag cgaagtagta cgcctggtgg ctgcgtcgaa tcccgacgta gcgatgtcgc
      481 gcagtgatcg gtggactcgg cggactagta cccgctcacg gcgctggcgg tgctcttcgt
      541 aggtgtggcc gatattgcaa cccacgttgt cgcgcccgct ccatcagttg cgcaccgtgc
      601 ccgtcgaaca tccgactctg ccgcaaatgg cgcgcagttt gcgcccggtg cggcagcgta
      661 acgagcaaat gctggcgatg cgaggatctc tcccgtgagc gcgacggtcg tcctgtatgt
      721 gaggcgactg gatgtgatgc gcgcgttcta ccaagagcgc ttcggcttga agatcaccga
      781 cgcaccgacg cctactgcgt cctcgaatcg gacgactgga cgctttgact cgtcgtgacc
      841 cgacacgctg tcaccgcggc gcaaatcgaa gttcccgatc caccgaccag gcgagacctg
      901 tcgccgatca agctgggatt tcgagtacgc cagatcgagg acctgcggcc agtcttcgaa
      961 agtctcggtg gccgtttgga cgcgggcacg gctgcctggg agttttatgg attcgcgcgc
     1021 ctcgacggtg tcgatccgga aggcaacgtc gttcagttgc tacagcccct tacacgctcg
     1081 tagcggcgat tccgcgcggc ccaacgtgtc gggctttgat ctggatcgcg gcccatgtac
     1141 tcatgcggca gcgttacgtc tgcggcggct gcactgagac tccaaaccat gagcaacagc
     1201 atcgcaggta gccccaccct caccgcggac gttatccgac agtcggcaac accgaacgcg
     1261 cgtcaatgcg gcatgagcgc gcattggttg gcggcctgtg cggcgtcgtc gctatttcga
     1321 cggactccac cgtgacccgt tcgtactcgg cagcacgaac gtgtcgatgc tttcggcgtg
     1381 acaaaggtca agcaggtgcg ggtcggaggt ggccagcggc acggcgcgtt cgtgggctgc
     1441 tgcggcagcg aggcaatcgg ccatgctcac cgcgcaccgg gtccggtggt aataacgcgc
     1501 ccgcagtcgc ccggcgaggc cggcggcttg tgcgtcgatc gggagagcat cgttcagccc
     1561 gagctcggcc aggtcaagga acacgtcgtc ttcgtcaact ccggccagcc ggatgaggtg
     1621 gtcgacgacc tcaccaagcc caactgcggt caacgacacg tcgtcggatc gcatcaacga
     1681 cgccacctca tcggcagcgg gttcgtcccg caagaaagag accaccgcat aggcatcgag
     1741 cacgatcaac tgcgctcacg gcctcgggta gaccgccgct cctgggtacg ggccgcatca
     1801 gccgagggac cccgatcgac gtacttgcct ttgatcgcgg cgaccggatc cacagatagc
     1861 ggccgcatca ccacccggtc gccgaggtcc acaaccacaa cctgccgggt gttccacctc
     1921 gctcgtgtct ctgcggggat cgacacctgg ccattacggg aaacggtcat cacacgttca
     1981 cgagactcca caaccctcat tgtatacgaa ctagtatccg ttgtatgccg tccgacaaca
     2041 cgaatgaccg tctctgcggc aaagtctccc gcgagagtgc acgcccccgc gccgcgtccg
     2101 cgctagttag cgctggccga tgaccggacc gcccgcatca agctgccctt ggcagatccg
     2161 aaggtgactg agcgatcccg agggttggcc gttggaagtc gtgatggttc cggtgccgtt
     2221 gatgtcgaga cggaacgtga ccggcacaac ttcgtcgctc gcagggcagt cgtagccgac
     2281 gcgggcgccg ttcgtgatgg aatccgacga ccccggcacc atgaaggcga cgcgctcacc
     2341 agacagcggc atggcgccgt tgggcaccgt agcggcgttg gcactgagga gaagcccgcc
     2401 cgccaccaca gcatgatcgg tgatcgtgtc gatggtcccg gtcgcgtcca gcccgacgat
     2461 gccgatggtg ccggttagct ggcacgcggc gccactcatg tctcggatga tcatccagcc
     2521 gtagtttgaa tcgcccgctt cctcgaagtc ttcgaggtcc gccgacagtt ggctcgctct
     2581 gcacgctggg ccgacaacaa ccgatggcgg agtggacacg ggcacgatct gcgagcccgg
     2641 agatgaggcc acgagtgagg gcgaagcggc aagagcattt ggcgacaagg aatccggagc
     2701 gatcaccggg gatgccggcg gctcggcaag tatgggcagc accggggcga cagcagtcga
     2761 ggaggagact gtgccaacgc cgggcgcagt gccaccttgg ctggtcgcct tggaaccgac
     2821 gatggagctt gcacagcctg acaggagtag cagcccgacc ccaaacatca agggtctacg
     2881 catcaaccct ctcaatgaca cgacgacact ctctcaaccc gcgtggatcg gatcggcgat
     2941 gtcgaggtgt cctccgcagg tcagcagact ggcgaagcca tcacctccgg ggtcgttgct
     3001 gatattgggt accgtcaaca tcccatcggc gaaacttagc tgccacgcgg ccggtgtgac
     3061 ttcgtcgccg acgcatgcgc cgcccgcgt
//
LOCUS       DATCBJ010000441         4811 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_479485, whole genome shotgun sequence.
ACCESSION   DATCBJ010000441 DATCBJ010000000
VERSION     DATCBJ010000441.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 4811)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4811)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4811
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_479485"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..522)
                     /locus_tag="VII50_08925"
     CDS             complement(<1..522)
                     /locus_tag="VII50_08925"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HEY5051011.1"
                     /translation="MAATVAMSSSVRLGHRRALDGIRGVAVVLVLARHVWPEGGAGMV
                     GVDLFFVLSGFLITCLLTEEWADNGAVSFTAFYARRALRLLPALYVMLAVFVACSLLF
                     ARGDRLEHALKSAGLAGTYLTGYAAFDNFSNSQALGPVWSLAVEEQFYLLWPLLLWLA
                     LRRGWSLRRCAGLA"
     gene            593..1015
                     /locus_tag="VII50_08930"
     CDS             593..1015
                     /locus_tag="VII50_08930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5051012.1"
                     /translation="MTKRRRIWVWCAAIAAVAVGLVAAGVIVGRSTAPVHDPGFAHGN
                     AAGYAEGVADGRSLQIGDSVPAGSKKAALAAFQAGYRAGETDAFGAYDGGWKLGTPYV
                     VILGKGVAGATYRFVERDQLTSGTAYQLCPDGTSLCHS"
     gene            complement(1035..2507)
                     /locus_tag="VII50_08935"
     CDS             complement(1035..2507)
                     /locus_tag="VII50_08935"
                     /inference="COORDINATES: protein motif:HMM:TIGR00711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY5051013.1"
                     /translation="MTTSMQAPVSAPGAARRGHHPGLALAVIAASQLMVVLDATIVNV
                     ALPTIRGALHFSYTNLTWVLNAYTLTFGGLLLLGGRAGDILGRRRVFIFGIALFTFAS
                     FLGGLATSSWWLLAARALQGVGGAIASPTALSLITTNFEEGEERNRAFGVFAAVSGSG
                     AAIGLLAGGMLTSWASWRWVLFVNVPIGIFIASIARQYINESERHPGRFDVVGAVTST
                     LGMTSLVYGFIRASGTAGWGDPVTILCFIAAVALLAGFLFNEMRSPQPITPLHMFKNR
                     NRSGTYVVMLLLAGALFAMFFFLTVFVQSILHYSPLKTGFIYLPLSAAIVVSAQICAK
                     QLVKVGPKPFLVVGAIMCAVALWWLSLVSVHSTYAANLLGPLIIFGLGMGFMFVPLTM
                     LAVSGVESEEAGSASGLLNVTQQVGGSLGLAILTTAFATAQRHAAAHGATGNAKLAHG
                     ISTALQVSVVFGVAAMLASIFLINAKASDLDPSAVPGMGG"
     gene            2615..3289
                     /locus_tag="VII50_08940"
     CDS             2615..3289
                     /locus_tag="VII50_08940"
                     /inference="COORDINATES: protein motif:HMM:NF028169.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR-like C-terminal domain-containing protein"
                     /protein_id="HEY5051014.1"
                     /translation="MPPARSRSSTTLATASDKNAGVPGERHVASKAVPLFRCRRRGVA
                     LETAIYEAVLQQLSTVGFGAMTIEGVAAAARTGKAAIYRRWPSKEELVADTLDNVLPS
                     MDLPPDTGNVRSDLAEIFTVMLEVMASPAGGAMQALMGELGHDHDFITTVHERVLAPR
                     KALMMEILVRGVARGDVRRDAIKPVVVEAGPALLVHRLLTFGPPIDPAYMEAVLDDVV
                     MPLISP"
     gene            complement(3306..4394)
                     /locus_tag="VII50_08945"
     CDS             complement(3306..4394)
                     /locus_tag="VII50_08945"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HEY5051015.1"
                     /translation="MGMWRSAASGRRGWATVRASRWTAPGASMWWLLGLAVAIAVLCP
                     VAGTYWIPPVAEALPILGGGLLLRRRELLALLAVAMLGIVYDEVVLGWHVVRAGQLVV
                     VIAVAIFAYRLARTRETLGVQGLRGESMLLELRDRLRAQGEMPVLPPGWQHEIVIRSA
                     GAGSFGGDFLVAARTGQGAVLEVVLVDVSGKGIDAGTRALLLSGALGGLLGAVPPERF
                     LPAANDYLLRQGWTEGFATAVHLVVDLSTGSYLVESAGHPPAAHFVKGSGTWRLIDSE
                     GTVLGVLAEASYTSVHGVLGSGDAMLLFTDGVVETAEREIAVGIDKLIGEAERLVTQG
                     FRDGAKQLVDRVAPDGSDDRALVMIWRD"
     gene            4458..4781
                     /locus_tag="VII50_08950"
     CDS             4458..4781
                     /locus_tag="VII50_08950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5051016.1"
                     /translation="MPRRHEPVTGKRNVTGTSAGDPTSTGVGQVSATAAGTGALRGLV
                     GAGPSQVGIVGALRARDVSRPTPDDIKRALAKPEPTARPGARQDTAPQDAAESGTGGG
                     TSVSS"
BASE COUNT          956 a         1621 c         1570 g          664 t
ORIGIN      
        1 cgcgagtccg gcacatcgcc ggagggacca ccccctgcgc agagcgagcc acaggagcag
       61 cggccagagg aggtagaact gctcctcgac agcaaggctc cacactggcc ccagagcctg
      121 gctgttggag aagttgtcga atgctgcata gccggtcaga taagtgccgg cgagaccggc
      181 cgatttcaac gcatgctcga ggcgatcgcc gcgcgcgaag agcagcgagc atgccacgaa
      241 gacggccagc atcacgtaca acgcaggaag cagccggagt gcgcgccggg cgtagaacgc
      301 ggtgaacgag acagcgccgt tgtcggccca ttcctcggtc aacaagcaag tgatgaggaa
      361 cccgctgagc acgaagaaca aatcgacgcc gaccatgccc gcgccaccct cgggccacac
      421 gtggcgggcc agcaccaaga ccactgctac gccccggatg ccatcgagcg cgcgacgatg
      481 cccaagtctc acgctgctcg acatcgcaac ggtcgcggcc atgcccgcga ggctaacggc
      541 agaggggacc cgccgtcccc ccgagcacgc cacagtgcgg caaggttggc gcatgacgaa
      601 gcgtcggcgg atatgggtgt ggtgcgcagc gatagccgcg gtggcggtgg gcttggtcgc
      661 ggcaggggtg atcgtcggtc ggtccacggc accggttcac gacccgggct tcgcgcatgg
      721 gaatgccgcc ggttacgcgg aaggcgtcgc cgacggtagg tcgctgcaga tcggcgacag
      781 cgtgccggcc gggtcgaaga aagccgcact ggcagcattc caagccggat accgagccgg
      841 tgagaccgac gccttcggcg cctatgacgg cggctggaag ctcggcactc cctacgtcgt
      901 catcctcggc aaaggcgtcg ccggcgcgac ctaccgtttc gtcgagcggg accagctcac
      961 gtctgggacg gcgtaccagc tatgtcccga cggcacgagc ctctgtcata gctagagcgc
     1021 tgtcatcgcg ctagctagcc gcccatgccc ggcaccgccg acggatcgag gtcggacgcc
     1081 ttcgcgttga tcaagaagat gctcgcgagc atggccgcga cgccgaagac gaccgacacc
     1141 tgcaacgcag tcgagatgcc gtgcgcgagc ttggcattcc cggtggcgcc atgagcagca
     1201 gcgtgccgct gcgctgtcgc gaacgcggtg gtcaggatcg ccaggccgag cgatcccccg
     1261 acctgctggg tcacgttcaa cagaccagac gccgagcctg cttcctcgct ctcgacgcca
     1321 gagactgcca gcatcgtcaa cggcacgaac atgaagccca tgccgaggcc gaagatgatc
     1381 agtgggccaa gcaggttggc ggcgtacgtg ctgtgcacac tgacgagcga gagccaccac
     1441 agggcaaccg cgcacatgat cgcgccaacc accaagaacg gcttcgggcc gaccttcacg
     1501 agttgcttgg cgcagatctg tgcactgacg acgatcgccg cgctgagcgg caggtaaatg
     1561 aacccggtct tcagcgggct gtaatgcagg atgctctgca cgaaaaccgt aaggaagaag
     1621 aacatcgcga acagggcacc ggccaggagc agcatcacga cgtaagtgcc cgagcggttt
     1681 cggttcttga acatatgcag cggcgtgatg ggctgcgggc tgcgcatctc gttgaagagg
     1741 aagccggcca acagggccac ggccgcaatg aagcacagga tcgtgaccgg gtcgccccag
     1801 ccagcggtgc ccgatgcccg gatgaaccca tagaccaacg acgtcatgcc gagcgttgac
     1861 gtcactgcgc caacgacgtc gaaccgacct gggtgacgct ccgactcgtt gatgtactgg
     1921 cgcgcgatgc tggcgatgaa gatgccgatc gggacgttca caaaaagcac ccagcgccac
     1981 gaagcccagg acgtcaacat gccgccggca agtagaccga tcgcggcacc agaaccggag
     2041 acggcagcaa acacaccgaa cgcccggttg cgctcttcgc cttcttcgaa gttcgtggtg
     2101 attagggaga gcgcggtcgg tgaagcgatc gcgccgccga ccccctgcag cgcacgtgca
     2161 gcgagtagcc accacgacga tgtcgccagg ccaccgagga acgaggcgaa ggtgaacaac
     2221 gcgataccga agatgaagac ccgccgccgg ccgagaatgt cgcccgcgcg gccgccgagg
     2281 agcagcaaac caccaaacgt caacgtgtac gcgttgagca cccacgtcag gttcgtgtag
     2341 gagaagtgca gcgcgcctct gatggtgggc aatgcgacgt tgacgatcgt cgcgtcgagc
     2401 accaccatca gctggctcgc cgcgatcacg gcaagagcga ggcccgggtg atggccgcgt
     2461 cgcgcggcgc caggtgcact cacgggcgcc tgcatcgagg ttgtcatgaa tctcctcgtt
     2521 ttcgtggaag gaacgctaac gttcactaag tacggacgct acattgcatg agatagagaa
     2581 cgcaaccgtt ccttcgctgg gtgaggaggc agccgtgccg cctgcacgtt cgcggtcctc
     2641 gaccacgcta gcaacggcct ccgacaagaa cgccggggtg cctggcgagc ggcacgttgc
     2701 gagcaaggcg gtcccattgt ttaggtgccg ccgccgcgga gtcgcactcg aaaccgccat
     2761 ctacgaagcc gtccttcagc aactcagcac cgtgggtttc ggcgcgatga cgatcgaagg
     2821 tgttgccgcc gccgcgcgca ccgggaaagc cgcgatctat cgccggtggc cgtcaaagga
     2881 agagctggtc gccgacaccc tggacaacgt gctgccgtcc atggatcttc cgcctgatac
     2941 cgggaacgtt cgcagcgacc tcgccgagat tttcaccgtc atgctcgagg tgatggccag
     3001 tcccgcgggg ggcgcgatgc aagcgttgat gggcgaactc gggcacgacc acgacttcat
     3061 caccaccgtg cacgaacggg tgttggcgcc acgcaaggca ctaatgatgg agatcttggt
     3121 gcgcggagtt gcgcgcggcg acgtgcgccg cgacgccatc aagccggtcg tcgtcgaagc
     3181 cggtcccgcg ctgctcgtgc atcgactact gaccttcggg cccccgatcg acccggccta
     3241 catggaggcg gtgctcgacg acgtcgtgat gccgctcatc agcccgtgac cggagctacg
     3301 aagagctagt cgcgccagat catcacgagc gcgcggtcat cgctgccgtc tggtgccacc
     3361 ctgtcgacca gctgcttcgc gccgtcccgg aagccctggg tcacgagccg ctccgcctcg
     3421 ccgatgagct tgtcgatgcc gaccgcgatc tcgcgttctg cggtctcgac gacgccatcg
     3481 gtaaacagca gcatcgcgtc gcccgaacca agcacgccat gcaccgacgt gtagctggcc
     3541 tccgccagca caccgagcac ggtcccctca ctgtcgatga ggcgccacgt gccgctgccc
     3601 ttcacaaaat gggcggcggg tgggtgccca gcggactcga caaggtagct gcctgtgctc
     3661 aggtcaacaa ccaggtggac ggcggttgcg aacccttcag tccagccctg ccggagcaga
     3721 tagtcgttcg ctgcaggcaa gaaccgctca ggcggaaccg cgccgagcag ccccccgagc
     3781 gcacccgaga gcaacagcgc tcgggtgccg gcatcgatgc ccttgcccga gacgtcgacc
     3841 agaacaacct cgagaacggc accctggccg gtccgtgcgg cgaccaggaa gtcaccgccg
     3901 aacgaaccgg caccagcaga gcggatgacg atctcgtgct gccaaccggg aggcagtacc
     3961 ggcatctcgc cctgcgcgcg caaccggtcg cgcaactcca gcaacatcga ttcaccgcgc
     4021 aaaccctgaa caccaagcgt ttcgcgggtg cgagccaacc ggtaggcgaa aatggccacg
     4081 gcgatgacga cgacgagttg gccggcccgc accacatgcc agccgaggac cacctcgtcg
     4141 tagacgatcc cgagcatcgc gaccgcaagc agcgcaagca gctcgcgccg ccgcagcagg
     4201 agtccaccgc cgaggatcgg caacgcctcg gccacgggcg ggatccagta ggtgccggcg
     4261 acagggcaaa gcacggcgat ggcgacggcg aggccgagca gccaccacat cgacgcgccg
     4321 ggcgccgtcc agcggctcgc gcgaacggtg gcccagccgc ggcgacccga tgcagccgag
     4381 cgccacattc ccatggtcgg gcagcgtaac ggccctaggc cggtgtgatc aggctcgacc
     4441 tgtggaacgc tctcgtcatg cccagacgtc acgaaccggt caccggcaag cgcaacgtca
     4501 ccggaaccag tgcgggcgac cccacctcga cgggggtcgg ccaggtatcc gcaacggctg
     4561 ccggaacagg tgcgctgcgc gggctggtcg gcgccggtcc ctcccaggtt ggcatcgtcg
     4621 gcgcgttgcg cgcgcgcgac gtctcgcgac cgacccccga cgacatcaag cgcgcactgg
     4681 ccaagccgga accgactgct cgacctggcg cccgtcagga cacggcccct caggacgcgg
     4741 cggagtccgg cactggcggc gggacgtcgg tttcttcgta gcggctgacc atcgccagcc
     4801 gccgaacgtg c
//
LOCUS       DATCBJ010000442         2089 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_519949, whole genome shotgun sequence.
ACCESSION   DATCBJ010000442 DATCBJ010000000
VERSION     DATCBJ010000442.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2089)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2089)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2089
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_519949"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1097)
                     /locus_tag="VII50_08955"
     CDS             complement(<1..1097)
                     /locus_tag="VII50_08955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5051017.1"
                     /translation="MTIHSDAAFDPAVPRFRWASPGSATVVGVLTLVLLVVGFPLSEF
                     AKQGLVNQDILMLPFAVVGFVVARKQPRNPVGWIMLLLAPIFMLSSDAGMYSLLIYQL
                     GHTTLPLGRLAVALTQLWIALLVLLPLPILLFPDGRLESRRWRWALWTYLAVCALILI
                     SAGVADLRAFTDRHIQVDSTGELVSQGQSKGNNLAASVFPLYAAFALAFVIRQFVVYR
                     RATGERRQQLKWLMAGGTIGIVAIATTLTFGGGMGFSGIIAVPVAIGIGILKYRLYDI
                     DRLISRTLSYAIVTGVLVGIYIGMVTLATRALPLSSPIGVAASTLTVAALFSPLRRRA
                     QRLVDRRFNRARYDADATIAAFAGRLRDEVDL"
     gene            complement(1174..1572)
                     /locus_tag="VII50_08960"
     CDS             complement(1174..1572)
                     /locus_tag="VII50_08960"
                     /inference="COORDINATES: protein motif:HMM:NF016152.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HEY5051018.1"
                     /translation="MASWDDVRRIALSLPDTTEAVSWGNAHWRVKNKGFVWERPLHQS
                     DRAALKALGEEVPEGALLGVRVADLGVKEAIIADDPKLFFTIPHFDGYPAILVRLDPI
                     GVPELEELIVEAWLDRAPKRLAQQYLDELP"
     gene            1696..>2089
                     /locus_tag="VII50_08965"
     CDS             1696..>2089
                     /locus_tag="VII50_08965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5051019.1"
                     /translation="MNVTRKSLIAGVTAAGLLVGGAGVAAAATGSSSPSTSSPASASA
                     LLASVSSGGSTGGVSVAGGVSLSAIQARVDARVAKVTGTLTKVQTRVDASAKLTAAQK
                     TTIDGDISTTLTDLATIKTDVDSATMLAG"
BASE COUNT          415 a          698 c          659 g          317 t
ORIGIN      
        1 aggtccacct cgtcgcgcaa tcgtccggcg aatgcagcga tcgtcgcgtc cgcgtcatac
       61 cgcgcccggt tgaagcggcg gtcgacgaga cgctgtgcgc gccggcgcaa cggcgaaaac
      121 agtgcggcga ccgtcaatgt cgaggctgcg acaccgatgg gtgacgacaa cggcagcgcc
      181 cgagtcgcca gcgtcaccat gccgatgtag atcccgacga ggacaccggt gactatcgcg
      241 taggacagtg tccggctgat caatctgtcg atgtcgtaga gccggtactt caagatcccg
      301 atgccgatgg cgaccggcac cgcgatgatt ccggagaagc ccatgcctcc gccaaaggtg
      361 agcgtcgtag cgatcgcgac gatgccgatc gtgccgccag ccatcagcca tttcagctgc
      421 tggcggcgct ctcccgtcgc tcggcgatac acaacgaact ggcggatcac gaatgccaag
      481 gcgaacgccg catacaaagg gaacacgctc gcagcgaggt tgttgccctt gctttgacct
      541 tgcgagacta attctccggt ggagtccacc tgaatgtgcc ggtcggtaaa cgcccgaaga
      601 tccgccactc ccgcggatat caagatgagc gcgcagaccg cgaggtaggt ccagagcgcc
      661 catcgccagc gccgcgattc gaggcgtccg tcgggaaaca gcaggatcgg gagcggcaac
      721 aagacgagca acgcgatcca cagctgcgtg agcgcaaccg caagccgtcc cagcggcagg
      781 gtggtgtgac cgagctggta aatcaacaac gagtacatgc ctgcatcgct gctgagcatg
      841 aagatcgggg ccagaagcag catgatccag ccgaccgggt tccgcggctg cttccgggcg
      901 accacgaaac ccacgacggc gaacggcagc atcaggatgt cttggttgac caggccctgc
      961 ttcgcgaact ccgacaacgg aaacccgacg accagcaaca cgagggtcag cacgccgacg
     1021 accgttgcgg agcccgggga tgcccagcgg aaccgtggca cggccggatc gaaggcagcg
     1081 tccgagtgga tcgtcacttc acctccgaag gctcctgcga gacctcatga tcaggcgacg
     1141 gtaatcgtcg atcgttccga gaacgctacg aaatcacggc aactcgtcga ggtactgctg
     1201 tgcgagccgc ttcggtgccc ggtccagcca cgcctcgacg atcagctcct ccagctccgg
     1261 gacgccgatc gggtcgagcc ggaccaagat cgccggatag ccgtcgaagt gcgggatcgt
     1321 gaagaagagc ttcggatcgt cggcgatgat cgcctccttg accccgaggt cggccaccct
     1381 gacaccgaga agtgcgccct cagggacctc ttcgcccagg gccttgaggg cagcgcggtc
     1441 gctttgatga agtggccgtt cccacacgaa acccttgttc ttgacccgcc agtgggcgtt
     1501 gccccacgac accgcttcgg tcgtgtcggg caaggacagc gcgatccggc gcacgtcgtc
     1561 ccagctggcc acgaccacga accctaatac ggacgccgcg gggtttacct gcctcgtaca
     1621 tgacgccgcg tggtcgcctg acgtaccggg ccgacattgc aggagataac cacgacaacg
     1681 actcaggaga tttccatgaa tgtcacccgc aagtccctca tcgccggcgt cactgccgct
     1741 gggctgctcg tcgggggcgc tggggtggcg gcggccgcga ccggcagctc gtccccctcc
     1801 acctcgagcc cggctagcgc ctcggcactc cttgcctcgg tctcctccgg tggctcgacc
     1861 ggcggggtct cggtcgccgg cggagtttcg ctatcggcga tccaagcccg cgttgacgcc
     1921 cgcgtcgcga aggtaacggg gacgctcacc aaggtccaga cgagggtcga cgcgagcgcg
     1981 aagctgaccg cggctcagaa gacgacgatc gacggcgaca tcagcaccac gctcaccgat
     2041 ctggcgacaa tcaagacgga cgtcgacagc gccaccatgc tcgccggcc
//
LOCUS       DATCBJ010000443         2136 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_229093, whole genome shotgun sequence.
ACCESSION   DATCBJ010000443 DATCBJ010000000
VERSION     DATCBJ010000443.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2136)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2136)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2136
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_229093"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..596)
                     /locus_tag="VII50_08970"
     CDS             complement(<1..596)
                     /locus_tag="VII50_08970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HEY5051020.1"
                     /translation="MSVTLPAPEQEPLQLVVLTGLSGAGRSTAAKCLEDLGWFVVDNL
                     PPGLLDTMVELGTRSGGAITKIAAVVDVRSRGFTSDLQSALTSLERRGAEPQVLFLEA
                     SDDELVRRFENVRRPHPLQGDGRLVDGIARERELLRDVRGEAGLVLDTTRLNVHELRA
                     KIVSAFADANEPTMRATVVSFGYKYGLPVDADLVVDCR"
     gene            complement(593..>2136)
                     /gene="uvrC"
                     /locus_tag="VII50_08975"
     CDS             complement(593..>2136)
                     /gene="uvrC"
                     /locus_tag="VII50_08975"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HEY5051021.1"
                     /translation="AEQTGRPCLLGHIGKCAAPCVGWVSIEEHRGIVEDFCDFMAGHP
                     DAYLKRLEREMKQAATEQEYERAARLRDDIAALRRAMEKQTVVLADATDADVIAMAED
                     QLEAAVQVFFVRGGRVRGQRGWVVEKVEDVTTAELVEHFITQLYGEVDELVGAAAEVP
                     REVLVPELPADLETVTEWLSVRRGSRVDFRVPQRGDKRALMETVARNAAQSLMLHKTR
                     RAGDLTARGRALQEIQDALQLDEAPLRIECYDISNLQGTDVVASMVVFEDGLARKAEY
                     RRFAITSVGSGALPPDPDPHAEPEPGDRATANDVAAIHEVITRRFRRYLDERAASDLQ
                     MDELPEPDETDPHGSVRTMIDPDTGRPRKFAYTPNLVVVDGGQPQVAAAKRALDELGI
                     DDIALVGLAKRLEEVWRPGDPDPVILPRSSEGLYLLQRVRDEAHRFAISYHRAKRSAS
                     MTRSALEGIPGLGSTRRAALLRHFGSVKRVRAATVDELAEVTGIGRGTAEVVHAALAG
                     KETNT"
BASE COUNT          338 a          758 c          681 g          359 t
ORIGIN      
        1 aaccggcaat cgacgacgag gtcggcatcg accggaagcc cgtacttgta gccgaaagag
       61 accaccgtcg cccgcatcgt gggttcgttg gcgtcggcga acgcagacac gatcttcgcg
      121 cgcagctcgt gaacgttaag gcgcgtcgtg tcgagcacaa ggccggcctc tccgcgcaca
      181 tcgcgcagca attcgcgctc gcgcgcgatg ccgtcgacca ggcgaccgtc cccttgcaag
      241 gggtgcggac gccggacgtt ctcgaagcgc cggacgagtt cgtcgtcaga cgcctcgagg
      301 aagagcacct gcggctcggc ccctcgacgc tcgagggacg tcagcgcgct ctgcagatcg
      361 gaggtgaagc cccgactccg gacgtcgacc acggccgcga tcttggtgat cgccccgccg
      421 cttcgggtgc cgagctcgac catcgtgtcg agcaagccgg gcggcaggtt gtcgacgaca
      481 aaccagccga gatcctcaag gcacttcgcg gcagtgctac gaccggcacc cgagagtccg
      541 gtcagcacga caagctgcag cggttcctgc tccggcgccg gtagcgtcac gctcacgtgt
      601 tcgtctcctt gcctgccagc gccgcgtgca ccacttccgc cgtgcctctc ccgatgccgg
      661 tgacctcagc gagctcatcg acggtcgccg cgcgcacccg cttcaccgat ccgaagtgtc
      721 gcagtaacgc cgcacgtcgg gtggagccga gcccgggtat tccttcaagg gcactgcggg
      781 tcatggacgc cgaccgcttc gctcggtgat agctgatcgc gaaccggtgc gcctcgtcgc
      841 gaactcgctg cagaaggtag aggccctcgc tgctgcgcgg caggatcacc ggatccggat
      901 ccccggggcg ccacacctcc tcgagccgct tcgcgagacc gacgagcgcg atgtcgtcga
      961 tcccgagctc atcgagggcc cgcttggctg cggccacctg cggttgaccg ccatcgacaa
     1021 ccacaagatt cggcgtgtac gcgaacttac gcggccgccc ggtgtcggga tcgatcatcg
     1081 tgcgcacgct gccatgtgga tcggtctcat ccggctccgg cagctcgtcc atctgcaggt
     1141 cgcttgccgc acgctcgtcg agataccgac ggaaccgccg ggtgatgact tcgtgaatcg
     1201 cggcgacgtc gtttgcggta gcccgatcgc cgggctcggg ctcggcatgc ggatcaggat
     1261 cgggtggcaa cgcaccgctg cccaccgacg tgatcgcgaa ccgccggtac tcagctttgc
     1321 gggcgagtcc gtcttcgaac acgaccatcg acgcgacaac gtcggtgcct tgcaggttgg
     1381 agatgtcgta gcactcgatc cgcaacgggg cctcgtcgag ttgcagcgca tcttgaatct
     1441 cctgcagggc acggccgcgg gcggtgagat cgccggctcg ccgggtcttg tgcagcatca
     1501 acgattgcgc cgcgttgcgg gccaccgtct ccatcagcgc acgcttgtcg ccgcgctgcg
     1561 gcacccgaaa gtcgacgcgt gatccgcgcc gcacactcaa ccattcggtg acagtctcga
     1621 ggtcggccgg cagttcaggc accagcactt cgcgcggcac ctctgcggcg gcgccgacca
     1681 gttcgtcgac ctcgccgtag agctgggtga tgaaatgttc gactagctcg gcagtcgtga
     1741 cgtcctcgac cttttcgacg acccaaccgc gctgccctcg cacccggccg ccacgcacga
     1801 agaacacctg cacggccgcc tcgagctgat cttcggccat cgcgatgacg tcggcgtcgg
     1861 tcgcgtcggc gagcaccacg gtctgctttt ccatcgcgcg ccgcagcgcc gcgatgtcgt
     1921 cgcgcaatcg ggcggcacgc tcgtactcct gctcggtcgc ggcctgcttc atctcccgct
     1981 cgagccgctt caggtacgcg tcggggtgtc cggccatgaa gtcgcagaag tcctcgacga
     2041 tcccgcgatg ctcctcgatc gacacccacc cgacgcaagg cgcggcgcat ttgccgatgt
     2101 gcccgagcag gcatggccgg ccggtttgct cggccc
//
LOCUS       DATCBJ010000444         2004 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_336788, whole genome shotgun sequence.
ACCESSION   DATCBJ010000444 DATCBJ010000000
VERSION     DATCBJ010000444.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2004)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2004)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2004
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_336788"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            446..1369
                     /locus_tag="VII50_08980"
     CDS             446..1369
                     /locus_tag="VII50_08980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5051022.1"
                     /translation="MEATYLDVGGPYGDSYAVGSDHVYFIDGTVVKAMARDGTVTDVG
                     YVPQVSTTATDEDVQSDTALAVSPDESTLVFGMPIAIQGDNGATSDRSQLWSDPLGGT
                     TAAATMVYNDANNDDQILLPFAWSSTDVWVSQLSTSGLGGAGPFLDYSHFDAATFDPA
                     THVLKPLTGDCVPSDDYAIDSSSPNVVCHGLPSDASVTVELPSGTRTVTMQPASAAYG
                     SIRVSDDGRCLAYGAYVGVFGNGHYNATVVDLTSGVLVSTVQGYTPMQWLNDDRLVVS
                     PSFAGGATYLLSPSFDSPVKLSSDQPTGALP"
     gene            complement(1345..>2004)
                     /gene="tmk"
                     /locus_tag="VII50_08985"
     CDS             complement(1345..>2004)
                     /gene="tmk"
                     /locus_tag="VII50_08985"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HEY5051023.1"
                     /translation="GKSTQVASLAGALRQQGHDVVTVRPDETPLGEILHSFVLQHQRG
                     PALEAWAEALLFTAERVQLLHETILPALRRGAVVIADRYADSTIAYQGGGRGIPTADL
                     LRLHAAACGDVWPDLTIYLAIPAATAAQRQRAQQLPLDRFEVAPETFHAAVEAAFEQL
                     AGEHPRRFVRIDATRPAILVSRDVEKVIVQRLPAAMPASPSPRSAGGFDPRSYGSAPV
                     G"
BASE COUNT          331 a          698 c          658 g          317 t
ORIGIN      
        1 cggccgccag cagcgcatgg cgcgaacctg gggtgaggcg cgcgagctgc acgcgctcgg
       61 atgggatccc ggtgcgcccg gtgttccggg tgttcgagtc catctcgcgg gaagccgcgt
      121 ggagcgcgtc gtgccggccg ctgacgtggt gctccgagcc gacgtcgagt agcgtgcggt
      181 gactcgcctg cactcgggcc tcacaggctt ggggctcggc gccatcgcgg tcatcgccgt
      241 gctcgcagcc tgcgggccgg cacacagccc cgcgcctccg acgaccgctg ccactgcgtc
      301 gccgacccca accccaacct cccttccaac accgtcgccg accacagcgc cacccgcgcc
      361 gttggtcgtg acctcccgcg aagccggacc ctcgggaacc ccctggacgg cagtgcaact
      421 ctcgctcgtc ggagaggacg gaactgtgga ggccacgtac ctcgacgtgg gagggccata
      481 tggcgactcg tacgcggttg gctccgacca cgtctatttc atcgatggga cggtggtcaa
      541 ggcgatggcg cgcgacggca cagtcaccga cgtgggatac gttccgcagg tcagcaccac
      601 agcgaccgac gaggacgttc agtcggacac ggcgttggcc gtatctccgg acgaatcaac
      661 gctcgtcttc ggtatgccga tcgcgatcca gggcgacaac ggcgcaacca gcgaccgctc
      721 gcaactctgg tccgatccac tcggcggcac gacagccgcc gcgacgatgg tctacaacga
      781 cgcgaacaac gacgatcaga tcctcctccc ctttgcgtgg agcagcaccg acgtctgggt
      841 gtcacagctc tcgacaagcg gcctcggcgg cgcggggccg tttctcgact actcgcactt
      901 cgatgcggcg accttcgatc cggcgactca tgtgctgaag ccgctcaccg gcgactgcgt
      961 tccgtctgac gactacgcca ttgactcatc gtcgcccaac gtggtgtgtc acgggttgcc
     1021 gagcgacgca agcgtcaccg tcgaactccc ctcagggaca aggaccgtca ccatgcaacc
     1081 cgcgagcgcc gcttacggat ccatccgggt atcggacgac ggtcgatgcc tcgcttacgg
     1141 agcgtatgtc ggcgtgttcg gtaacggcca ctacaacgcc accgtcgtcg acctcaccag
     1201 cggtgtgctt gtctcgaccg tgcagggcta tacgccgatg cagtggctca acgacgaccg
     1261 actcgtggtg tcgccctcat tcgccggggg cgcgacatac ctgctttcac caagcttcga
     1321 cagcccggtc aagctgtcgt ccgatcagcc gaccggcgcg cttccgtaag aacgcggatc
     1381 gaacccgccg gccgaccgcg gcgagggcga tgccggcatt gccgccggga gccgctgcac
     1441 gatgaccttc tcgacgtcgc gggagacgag aatcgccggc cgggtcgcat cgatacgtac
     1501 gaaccggcgt ggatgctcgc ccgccagttg ctcgaaggcg gcctcgacgg ccgcatggaa
     1561 cgtctcgggc gcgacttcga agcggtcaag cggaagttgc tgcgcgcgct gcctctgcgc
     1621 ggcggtggcg gctggtatcg caaggtagat ggtcaggtca ggccagacgt cgccgcacgc
     1681 ggcggcgtgc agccggagca ggtccgcggt ggggatgccc cgtccgccgc cctggtaggc
     1741 gatcgtcgaa tcggcgtagc ggtcggcgat caccacggct ccacgacgga gcgccggaag
     1801 gatcgtctcg tgcagcagct gcacgcgctc ggctgtgaag agaagcgcct cagcccaggc
     1861 ttcgagcgcc ggcccccgtt gatgctgcag cacaaagcta tgcagaatct cgccgagtgg
     1921 cgtctcgtcg ggccgaacgg tgaccacgtc atgaccctgc tgacgcaatg cgccggccag
     1981 cgacgcgacc tgcgtgctct tgcc
//
LOCUS       DATCBJ010000445         2539 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_370751, whole genome shotgun sequence.
ACCESSION   DATCBJ010000445 DATCBJ010000000
VERSION     DATCBJ010000445.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2539)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2539)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2539
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_370751"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(14..1993)
                     /locus_tag="VII50_08990"
     CDS             complement(14..1993)
                     /locus_tag="VII50_08990"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HEY5051024.1"
                     /translation="MHDHPRSDSEDVPDPPVEREVTPTGELAGRPARVARSLRFWISP
                     AGQPSWARPVLLLIATISAVAYSVGAARASVEPFYGAAARSVSQSWHDFVFGAFDPAG
                     TITVDKLPGALWLQALSLRLFGFHIWALVLPQIVEGVVTILVLYRAVRLIATPLAGII
                     ASSVLAVSPITVLLNRGNISDSLLIMCLVLAADAATRAIVIKRLRPLLAASVWVGVAF
                     QAKMAQAWLVLPGIWLAYAVAGAPSMRRRVAHVGAAALVTAAVSLSWMSAVSLVPRHD
                     RPFVDGSQHDSVFAQVFEYNGVLRFRNGGTGIGDPAPFLVQLVHQGRSLGLATSNVPP
                     GWHRLLTGVLGRDGAWLIPAALLSLALIGWSRRRTDRRDPVRAAAVLWGCWLAFTFVA
                     FSEGRYLNSYYVAALSPAVAAIVALGTTEVVQARVDVRRSSVVASGVIATSGYALYLV
                     HRGSDVPAWLVPTLAAATAVAVVGLVLVLRPHKAIVGVVTAVAVLLASLLAPAVASAT
                     SVTRVLGPFDSPFEPHSVTLVTQDLSRTLPEHIASGKRLRALDQPLRLSTETSGLAAN
                     DILFSGREVLPIGGFTGAVPSPTLSQLQHDVAVGTVNFFVLATSPVTPDPRLAWIRSH
                     CDLSGLAGREGSVTFGQYVCNVGPVASQAAPVPAG"
     gene            complement(2058..2279)
                     /locus_tag="VII50_08995"
     CDS             complement(2058..2279)
                     /locus_tag="VII50_08995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5051025.1"
                     /translation="MTLLALLSQSTPHTAAHVVASLPKPKAKVTVPIIVIFAVLVFIG
                     ISRKVIKLAVLAAIVCVIFLIYQSGAFSK"
     gene            complement(2292..>2539)
                     /locus_tag="VII50_09000"
     CDS             complement(2292..>2539)
                     /locus_tag="VII50_09000"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY5051026.1"
                     /translation="LLEPLGPDTPVGRFLARRGEGLHHVGYAVDNVRLAMETLALQGV
                     RVLDSQPRHGSLGASIAFAHPKDLSGVLTELVQAAQG"
BASE COUNT          531 a          801 c          825 g          382 t
ORIGIN      
        1 ccgcccccgg cgatcatcca gcagggaccg gagccgcctg gcttgccacc gggccgacgt
       61 tgcagacgta ctgcccgaag gtcacgctgc cctcccgacc cgccagaccg gacaagtcac
      121 aatggctccg tatccaggcc aggcgcggat caggtgttac cggagaagtt gcgagtacga
      181 agaaattcac cgtaccgact gccacgtcgt gttggagttg actgagcgta ggagacggga
      241 ccgcaccggt gaacccaccg atgggcaaga cctcgcgacc ggagaacaag atgtcgttcg
      301 ccgccaaccc gctcgtttcg gtcgagaggc gcagcggctg atctaacgct cgtagccgct
      361 tgcccgacgc tatgtgctcc ggaagagtgc gtgacaagtc ctgggtgacc aaggtcaccg
      421 agtgtggttc gaacggtgag tcaaatgggc cgaggactcg cgtcacggag gttgctgacg
      481 cgacggcggg cgccagcaac gacgccagca acacggcgac cgccgtcacg acgccgacga
      541 ttgccttgtg cggccgcaac acgagcacga gcccaacgac tgcgacggcg gtcgcggccg
      601 cgagtgtagg taccagccag gcgggcacgt ctgacccgcg atgtaccaga tacaaggcat
      661 aaccggacgt cgcgatgacg ccgcttgcca cgacggacga tctccgaacg tcaacgcgtg
      721 cctgcacaac ttctgtcgtg cccagggcga cgattgcggc gacggccggc gatagcgccg
      781 caacgtagta ggagttcaga tatcggccct cgctgaatgc gacgaaggtg aaggcgagcc
      841 agcaacccca taagaccgct gccgcgcgaa cgggatcgcg tcggtccgtt cggcggcgcg
      901 accacccgat cagcgccaag gaaagaagag cggccggtat cagccaggcg ccatcgcgac
      961 caagaacccc agtcaggagg cgatgccacc ccgggggaac gttggacgtc gcgagtccga
     1021 gggatcggcc ttgatgcaca agctgtacga ggaacggtgc agggtcccca attcctgttc
     1081 caccattccg gaaccggaga accccgttgt actcaaagac ctgcgcgaag accgagtcgt
     1141 gctgactacc gtcgacgaac ggccggtcgt gtcgcgggac cagggaaacc gcgctcatcc
     1201 agctcagaga tacagccgcg gtgacgaggg cggccgcacc gacatgcgcg acgcgtcgtc
     1261 gcatgctcgg cgcgccggcg acggcatacg cgagccagat tccgggcaac acgagccacg
     1321 cttgggccat cttcgcttgg aacgcgacgc cgacccatac ggacgccgcg agtagcggcc
     1381 gcagtctctt gatcacgatg gcacgggtcg ccgcgtcagc ggccaatacc aggcacatga
     1441 tgagcagcga atccgagatg ttgcctcgat tcagcagcac cgtaatagga ctgaccgcca
     1501 ggacactcga ggcgatgatt cccgctagag gtgtggcaat gagccgtacc gcgcgataca
     1561 gcacgaggat cgtgacgaca ccctcgacga tctgcggcag caccagcgcc cagatgtgga
     1621 agccgaagag gcgaagcgac agcgcctgca gccataacgc tccaggcagc ttgtcgacgg
     1681 tgatcgttcc tgctggatcg aaggcgccga aaacgaagtc gtgccaactc tgcgacacgc
     1741 tgcgcgcagc tgcgccgtag aagggctcta cggatgcgcg tgctgcaccg acgctgtaag
     1801 caactgccga aatcgttgcg atgaggagta gaacggggcg agcccacgaa ggctggccgg
     1861 ccggcgagat ccagaaccgg agcgaccttg ctacccgtgc gggccgacca gcgagttcac
     1921 cggtcggggt gacctctcgt tcgacgggtg gatcgggcac gtcctcgctg tccgagcgtg
     1981 ggtgatcatg cacgaactag ccaccaatta ggcctgcggc taccagtttt gtgcatgatc
     2041 accgggaaac aagcccgcta cttgctgaag gcgcctgatt ggtagatcag aaagatgacg
     2101 cagacgatcg cggcgagcac ggccagcttg atcaccttgc gcgagatccc gatgaacacc
     2161 agaaccgcga agatgacgat gatcggcacc gttactttgg ccttcggctt gggcaaggac
     2221 gcaactacat gcgcggccgt gtgcggcgta ctctgagaca gcagggcgag aagcgtcatg
     2281 ctcgcattct ctcagccctg cgcggcctgg acgagttccg tcagcactcc ggagaggtct
     2341 ttcgggtgcg cgaaggcgat cgacgcgccg agcgatccgt ggcgtggttg cgagtcgagt
     2401 acccgaacgc cctggagggc aagcgtttcc atcgcgagcc ggacgttgtc cactgcatat
     2461 ccgacgtggt gcaatccttc gccgcgcctg gcgagaaacc tgcccaccgg cgtgtcgggt
     2521 ccgagcggct caagcaatt
//
LOCUS       DATCBJ010000446         2338 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_409215, whole genome shotgun sequence.
ACCESSION   DATCBJ010000446 DATCBJ010000000
VERSION     DATCBJ010000446.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2338)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2338)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2338
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_409215"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..289)
                     /locus_tag="VII50_09005"
     CDS             complement(<1..289)
                     /locus_tag="VII50_09005"
                     /inference="COORDINATES: protein motif:HMM:NF017049.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5051027.1"
                     /translation="MHHGAGGVGPCRRSPACDVVRGFLRVWASQSGWASATAGGSPGP
                     PASGPVSTLPVGGPISVEPRINDRIRVPEVRLVGPNGEQVGIVRIEDALRLA"
     gene            724..1224
                     /locus_tag="VII50_09010"
     CDS             724..1224
                     /locus_tag="VII50_09010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5051028.1"
                     /translation="MAGPAAATFTTNTTDCAGSATIVGTGSDAGKSVSVNANDAKVTM
                     LREGKVNWEGSVPTATHNNQGDVVIHLGLVSVTAKTWGPSKNASNKVSRSGVARIPSA
                     LKLVPPGEYHMTGYNSSDEGTCAGSMELIFTGSILSSPAGIIAVIGTVLGAIGVVFAG
                     IAKALP"
     gene            1221..1442
                     /locus_tag="VII50_09015"
     CDS             1221..1442
                     /locus_tag="VII50_09015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5051029.1"
                     /translation="MKRRGRPVLGAISGLIFGGFLTIDLQIMNVWKLSIASEIVLPLV
                     GLAIGLALGLTGVVPRPGRSDGDAHAARH"
     gene            complement(1443..>2338)
                     /gene="hisG"
                     /locus_tag="VII50_09020"
     CDS             complement(1443..>2338)
                     /gene="hisG"
                     /locus_tag="VII50_09020"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR00070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HEY5051030.1"
                     /translation="IREATTVLSLVLPKGSLEAATLELFEAADLRVSRGSDRDYHASI
                     DDPRIDTVRFLRPQEIPTYVEQGLFDLGITGRDWITETDADVVSVGELEYSKATSNPV
                     RIVLAVVKEHAANRGDELPDGVRVSTEFPQLTKRYFEAIGVKAKIVPSYGATEAKVPD
                     IVDAIVDLTETGTSLRKHGLKIIDTLLTSRTELITNAAAYADAEKRAAMDDVMLLLRG
                     AVRARGNVLIKLNVADTQLAAVLAVLPSMGSPTVTALASGDMHAVESVVPKRGVNTLI
                     PTLKAAGARDILEIAISKIVE"
BASE COUNT          411 a          780 c          755 g          392 t
ORIGIN      
        1 cggccagacg cagcgcgtct tcaatgcgga caatgccgac ctgctcgccg ttgggaccga
       61 cgagccgaac ctcgggaaca cggatccggt cgttgatgcg gggctcgacg ctgatgggac
      121 ctcctactgg aagggtggac acaggcccac tggcgggcgg acccggcgat ccgccggcgg
      181 ttgcgctcgc ccatccagac tggcttgccc agacacgcaa aaagccccgc acgacgtcgc
      241 atgcagggct cctacggcaa ggcccgacgc cgcctgcacc gtggtgcacg cgtcgccaca
      301 aaccgcgtca cctgaccgcg gcggccgacc gaaccttccg acgcgcttgc cggagaggtg
      361 ggaggaagcc tccacttgcg gataccacgc gtgggcgcgg caccggtcgc cccaagagac
      421 taccaggcaa tctgggcccg ctcgccgagc cggcgtccgg cacccggctc gccggcgtcg
      481 ccgaagccat gtcgtcagaa ttgactattc aaccgggacg ccgcgacgtg gtttcgtcac
      541 acaggtccga tcagtcggca acgtcggctc atggaatttg ggggcgggac tccacctatg
      601 cgtttcaccc cggctcgtgc gtcccgggct gcgcgcacgc gtccatcgcg atggcgcggc
      661 agccgtcggt gggcagtgcg actggtcggg gcggtggtcg tgggcggagg cagtctgctt
      721 gccatggcgg gacccgctgc cgcgaccttc acgaccaaca ccaccgactg cgctggctcc
      781 gcgaccatcg tcgggaccgg gtcggacgcc ggtaagtcgg tttcggtgaa cgccaacgat
      841 gccaaggtca ccatgctccg cgagggcaaa gtaaactggg aaggcagcgt cccgacggca
      901 acccacaaca accagggcga cgtggtcatc cacctcggcc tggtcagtgt cacggcgaag
      961 acctggggcc cgtcgaagaa tgcgtcgaac aaggtctccc gatcaggcgt cgcgagaatc
     1021 ccgtctgcgt tgaagctggt gccacccggc gagtaccaca tgaccggata caacagcagc
     1081 gacgaaggca catgcgccgg gtcgatggag ctgatcttca cgggttcgat cctgtcgtcg
     1141 ccggccggca tcatcgcggt gatcggcacc gtgctgggcg cgatcggtgt cgtgttcgcc
     1201 ggtattgcga aggcgctgcc atgaagcggc gagggcggcc ggtactcggc gccatcagcg
     1261 gcctcatctt cggcgggttc ttgacgatcg acctgcagat catgaacgtc tggaagttga
     1321 gcatcgccag cgaaatcgtg ctcccgctgg tgggcctagc gatcggcctg gccctcggcc
     1381 ttaccggtgt ggtcccgcga ccggggcgct ccgatggtga tgcccacgca gcccggcact
     1441 gatcactcga cgatcttcga gatcgcaatc tcgagaatgt cgcgcgcacc ggcggccttc
     1501 agcgtcggga tcaaggtatt gacaccgcgc ttgggcacca cggattcgac cgcgtgcatg
     1561 tcgcccgagg ccaaggccgt cacggtcggc gaacccatcg atggcagcac cgcaagcacc
     1621 gcggccaact gcgtatcggc aacattgagc ttgatgagga cgttgccacg agcccgcacc
     1681 gcaccacgaa gcagcaacat gacgtcgtcc atcgcagctc gcttttccgc gtcggcgtac
     1741 gccgccgcgt tcgtgatcag ctcagtacgc gacgtcagca acgtgtcgat gatcttcagg
     1801 ccgtgcttgc gtaggctggt gccagtctcc gtgagatcga cgatggcgtc gacgatgtcg
     1861 ggcaccttcg cctcggtcgc accgtagctc ggcacgatct tggccttcac accgatggcc
     1921 tcgaaatagc gcttcgtcag ttgcgggaac tcggtcgaca cccggacacc atccggcagc
     1981 tcgtcgcccc ggttcgccgc gtgctccttg accaccgcga gcacgatccg taccggattg
     2041 ctcgttgcct tcgaatactc gagctcgccc acagacacga cgtcggcatc ggtctcggtg
     2101 atccagtcac gcccggtgat accaaggtcg aacaagcctt gctcgacgta ggtcgggatc
     2161 tcctgcgggc gcaggaaccg caccgtgtcg atccgtgggt cgtcgatcga cgcgtgatag
     2221 tcacggtcag acccgcggct gacccgcaag tcagctgctt cgaacagttc aagtgtggcg
     2281 gcttcgagtg agcccttggg cagaactagc gacagcacgg tagttgcctc tcggatcg
//
LOCUS       DATCBJ010000447         6733 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_308181, whole genome shotgun sequence.
ACCESSION   DATCBJ010000447 DATCBJ010000000
VERSION     DATCBJ010000447.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 6733)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6733)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6733
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_308181"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..782
                     /locus_tag="VII50_09025"
     CDS             <1..782
                     /locus_tag="VII50_09025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5051031.1"
                     /translation="AGEQTYGVVDSKGNLTGTESANIPAERAWSWDYLATLYKALGFP
                     DETTAALALIAGDGGGYSSLEPRPSYQNGISGFNARQYFTPTTFTQIAPGLTEPTDFT
                     FTANPSLSSGFQNSGRGEPDVSTNADPQTGYADYDPTLFAAAGGFEQYGGTSFVSPQL
                     NGTTAVIDSYVGHRVGFWNPKIYQWANGGNSPFTPLNDTAVFSGKQYLFQTNLKGVST
                     ALPGVFSNNNEYYTGKPGATWNPASGLGVPNLTALAKDFAK"
     gene            974..2698
                     /locus_tag="VII50_09030"
     CDS             974..2698
                     /locus_tag="VII50_09030"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEY5051032.1"
                     /translation="MAVTAGLLYMAGGIVTLVAFSFPHQPGVHAGFVRAIGVLALASG
                     AAIFWQGHRVPRWGYHLLLLAGDALIVAAVYESGGGVTSVACTTLFVFVTVDVFFFFA
                     WPWALVHLAITIAACVGSLAAVGALGDGQAIITPGVLIAVSLVVGRLVRAADDAEIDP
                     LTGLLNRRGIDRVLAEALGRAASTGTVISLALLDIDHFKLVNDSGGHISGDRLLRSIA
                     NSWRALSREHEVLGRYGGDEFALVLQDSALGDASTPTERLRTAVPGGMTCSAGIAAWE
                     PGDTLPMLLARADAALYDSKRAGRNRTSSRSGNRAAEAMQLRQAIADGELRAFYQPVV
                     DLRTGVVTGVEALVRWLRPGLNGPQLIAPDSFIPLAEESGVIGILGKWVLEAACGQAA
                     LWSSQGLPLTVNVNVSGRELVDGGYADSVLAMLADVGLPPSQLVLEVTESALEGSSEQ
                     AVSALSTLRTFGVRVALDDFGTGYSSLSRLDRLPVDIVKIDRSFVMTLPPDATRAPLV
                     AAIIAMAGAMGLQVVAEGIEAPHQARLLETLGCDEGQGFWLGRPLPAHQVDLSRRLTI
                     DVRDSLTA"
     gene            complement(2699..3220)
                     /locus_tag="VII50_09035"
     CDS             complement(2699..3220)
                     /locus_tag="VII50_09035"
                     /inference="COORDINATES: protein motif:HMM:NF037675.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HEY5051033.1"
                     /translation="MTRDSAATKARILKAATAEFAERGLAGARVDRLAASAGANKQLI
                     YAYFGSKEGLFDATLEANLETLLDGVPFDALDLRGYARRLFDFNVAHPELARLARWHS
                     LERPGMFMQLPVTGASLSRKIAALAAAQKAGRIDDTLPPPQLLVMLLAVIHAEGAERE
                     AVALAVQQLIARK"
     gene            3411..4247
                     /locus_tag="VII50_09040"
     CDS             3411..4247
                     /locus_tag="VII50_09040"
                     /inference="COORDINATES: protein motif:HMM:NF004826.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HEY5051034.1"
                     /translation="MTPSRVAIVTGASSGIGHASALRLQAAGFTVYAGARRVEKMKDL
                     ADAGVHPVDLDVTDDASMVALVDRVIADTGRIDVLVNNAGYGSFGAVEDTPIAEGRRQ
                     FDVNVFGLARMTQLVLPHMRARRSGRIINMSSMGGRISEPLGGWYHATKFAVEGMSGS
                     LRVELRPFGIDVVLIEPGGMNTEWGEIAANGLIAASGSTAYADQAPGVAKMLRSIDEK
                     PRPSASPDRIAKAVTRAATARRPRVRYLLGPGAKPLVTLQTILPDRVFDAMMARIFGT
                     PR"
     gene            4333..5880
                     /locus_tag="VII50_09045"
     CDS             4333..5880
                     /locus_tag="VII50_09045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5051035.1"
                     /translation="MPGISVRSGTAAIAIVTAGVLLAGCASKSTAAGSGNQPPKLLIG
                     TGTSSGNAAAPMMAAAGVPARGAAIAPMPIGGFGGFGGYVLSGTLPTQPTHAPIWTWQ
                     SGKASESDVTKLATALGLTGTPTRHAYGWDLTTSAGDLRVRDGDGEQWSYNRADAVPC
                     PAYQTDIDNADGASVGYGCAVSAPPPVATPGTTTSGTTAIVTPIDPVQGPDETATKAA
                     AASLLSTLGVTGSEQFNESSPASTLSVAPQIDGMPTQGIETSVDVDAKGIRAATGRLV
                     APKAGTDYPLQTAAAAFKSLADRPMPMIAQYCGPIPVDGGPGLPVPAASGPNTKFTLN
                     PGASAVPAPTVTGPNTKFPLNPAAPASPNTVMVVSAPPAPLSAPAVDSTGPVAVPPVE
                     SAYPCPTPVPQKVTGAVIGLQVQYDASGATTSADGSTSGGSNILVPTWFFTVEGSTDP
                     LTVIAVDPSFLGDQTIPPVGAASAGSASGGFTGSGGGSAGSGTVGVPPTVASAGPALP
                     PAPAAKP"
     gene            6023..>6733
                     /locus_tag="VII50_09050"
     CDS             6023..>6733
                     /locus_tag="VII50_09050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5051036.1"
                     /translation="MPRLGAWTSSLRRRGMWGLSRPRWDAVAKRSIRAAIVIPALFAF
                     ADKVLKDPNIATFASIGAFSVLLLADFGGPLRRRFIAYAGLAVVGAVFITVGTACSRT
                     TWLAVVVAAIAAALVLFLGILSGYVAKGSTASLLVLILPLTLPAHASAIGPRLEGWAL
                     ASVVGTLSVFLVWRDPPQAGLGTLAGNALHRLADHLRATLDGEPVEENSEAAEQAVAK
                     LKGAYLATPFRPAGATLSE"
BASE COUNT         1102 a         2400 c         2148 g         1083 t
ORIGIN      
        1 cggccggcga gcagacgtac ggggtcgtcg actcgaaggg gaacctcacc ggcaccgagt
       61 cggcgaacat cccggccgag cgagcatgga gctgggacta cttggccacg ctctacaagg
      121 cgctcggctt ccctgacgag acaaccgcag cactggcgct catcgccggc gacggcggtg
      181 gctacagctc gcttgagccg cggccgagtt accagaacgg catctccggc ttcaacgctc
      241 ggcagtactt cacgccgacg acgttcaccc agatcgcgcc cggtctcacc gagccgacag
      301 acttcacctt caccgcgaac ccgtcgttga gcagcggttt ccagaacagc gggcggggcg
      361 agcctgacgt ctcgaccaac gccgacccgc agaccggcta cgccgactac gacccgacgt
      421 tgttcgccgc ggccggcggt ttcgagcagt acggcggaac cagcttcgtg tcaccgcaac
      481 tcaacgggac gacggcggtg atcgacagct acgtcggcca ccgggtcggt ttctggaacc
      541 cgaagatcta ccagtgggcg aacggcggca actcgccgtt cacaccgctg aacgacaccg
      601 cggtgttctc tggcaagcag tacctcttcc agaccaacct gaagggtgtc agcaccgcgc
      661 tgccgggtgt cttcagcaac aacaacgagt actacacggg caagccaggc gcgacctgga
      721 acccggcatc gggcctcgga gttccgaacc tgaccgcact agcgaaggac ttcgcgaagt
      781 aacccaagga agccgccaca cggttcttgc agaccgctcg cctcgtgccc cgagggtgaa
      841 tgtctgcaag aaccgtgggg gtggccgata gggggacatg agactggcgc aaccggcgga
      901 ggcacgtcct cggctgccca gactcacccg gcccggcctc gcggttgctc cgtccatcgc
      961 cagtccacgc gcgatggccg tcacagccgg cctgctctat atggccggcg gcatcgtgac
     1021 gctggtcgcc ttcagcttcc cgcaccaacc cggcgtccac gcaggcttcg tccgcgccat
     1081 cggcgtgctc gccctcgcct ccggggccgc catcttctgg caaggccacc gcgtaccgcg
     1141 gtggggctac cacctgctgt tgctggcggg cgatgcactc atcgtcgccg cggtctacga
     1201 atccggcggc ggcgtgacct cagtcgcctg cacgacactg ttcgtcttcg tgacggtcga
     1261 cgtcttcttc ttcttcgcat ggccgtgggc tctcgtgcat ctcgccatca cgatcgccgc
     1321 ctgcgtcggg tcgctcgcgg cggtcggcgc actcggcgac ggccaggcga tcatcacccc
     1381 cggcgttctg attgcagtct cgctcgtcgt cggacgcctc gtgcgcgccg ccgacgacgc
     1441 ggagatcgac ccactcaccg gactgctgaa ccgtcgcggc atcgaccgtg tgttagccga
     1501 ggcgctcggc cgggctgctt cgactggcac ggtcatctcg ttggcgctgc tcgacatcga
     1561 ccacttcaaa cttgtgaacg acagcggcgg acacatcagc ggagaccggc tgctgcgctc
     1621 gatcgcgaac tcgtggcgcg cgctttctcg cgaacacgag gttctcggac gctacggcgg
     1681 cgacgaattc gccctggtac tacaggactc cgcgttgggc gacgcatcga cgccgaccga
     1741 gcgactgcgc acggcagtgc ccggcggcat gacctgctcc gccggcatcg ctgcatggga
     1801 acccggcgac acgctgccca tgctgctggc ccgcgccgac gccgccctct acgactccaa
     1861 gcgcgccggc cgcaatcgca cgagctcgcg cagcggcaac cgcgctgccg aggcaatgca
     1921 actacgacaa gccatcgcgg acggcgagtt gcgcgcgttc taccaacccg tggtcgatct
     1981 gcgcaccggc gtcgtgaccg gcgtcgaggc gttggtgcgg tggctgcggc ccggactcaa
     2041 cggcccgcaa ctcatcgcgc ccgactcgtt catccccctc gcagaagaga gcggcgtcat
     2101 cggcatactc ggcaagtggg tgctcgaggc cgcatgcggt caggccgcgc tgtggtcgtc
     2161 acaaggcctg ccactgaccg tcaacgtcaa cgtctcgggc cgcgaactcg tcgacggcgg
     2221 gtacgccgat tctgtgctcg cgatgctcgc ggacgttggg ctaccgccga gccaactcgt
     2281 gctcgaggtc accgagagtg cgctggaagg cagctccgaa caggctgttt ccgcattgag
     2341 cacgttgcgc accttcggcg tccgcgttgc actcgacgac ttcggcaccg gctactcgag
     2401 cctttcgcgg ctcgaccggc tccccgtcga catcgtcaag atcgaccgct cgttcgtgat
     2461 gaccttgcct cccgacgcga cacgcgcacc gctggtcgcg gccatcatcg cgatggccgg
     2521 cgcgatgggt ttgcaggtcg tggccgaggg aatcgaagcg cctcatcagg cccggctcct
     2581 tgaaacgctc ggctgcgacg agggccaggg cttctggctc ggacgtccgc tgccggccca
     2641 ccaggtggat ctctcccgcc gcctgacgat cgacgtgcgg gactcgctca cagcgtagct
     2701 acttgcgggc gatgagttgt tggacggcga gggcaaccgc ctcacgttcc gcaccctctg
     2761 catgaatgac cgcaagcaac atcacgagca gttgcggcgg cggcaacgtg tcgtcgatgc
     2821 ggccggcctt ttgtgctgct gcgagcgcgg caatcttgcg gctcagcgac gcgccggtga
     2881 ccggcaactg catgaacatg ccagggcgtt cgaggctgtg ccatcgcgcg agccgggcaa
     2941 gctcggggtg cgcgacgttg aagtcgaaca gccgccgcgc gtaaccccgc agatccaacg
     3001 catcgaacgg gacgccgtcc agcagcgtct cgagattggc ttcaagcgtc gcgtcgaaca
     3061 ggccttcctt gctgccgaag tacgcgtaga tcagctgctt gttcgcgccc gcactcgccg
     3121 cgagccggtc gacgcgggca cccgccagcc cccgctcggc gaattccgcc gtcgcggcct
     3181 tgaggatgcg agccttcgtg gcggcggagt cgcgcgtcat ggggtggcgg ccgaatcgcg
     3241 cgtcatggcg agcagtgtac gagcgatcca accaaccagt tgacaactcg gcgtccactc
     3301 gctagtttcc aaccaaccag ttagatgttc ctggagttca gctaacttgg aagctcgtcg
     3361 gcgctttctt aagcgtccac gagcttccaa cttcgggcgg gaggagcgcg atgacaccga
     3421 gtcgggttgc gatcgtgact ggcgcgtcgt ccggcatcgg gcacgcatca gccttgcgcc
     3481 tgcaggccgc tggcttcacc gtctacgccg gcgcgcgccg cgtggagaag atgaaggatc
     3541 tcgccgacgc cggcgtccac cccgtcgacc tcgacgtgac ggacgacgcc tcgatggtcg
     3601 ccctcgtcga ccgggtgatc gctgacaccg gacgcatcga cgtcctggtg aacaacgccg
     3661 gctacgggtc tttcggcgcg gtcgaagaca cgccgattgc cgagggacgc agacagttcg
     3721 acgtcaacgt cttcggtctg gcgcgcatga cgcagctcgt gctgccgcac atgcgggccc
     3781 gcaggagcgg tcgcatcatc aacatgtcgt cgatgggcgg ccgcatttcc gagccgctcg
     3841 gcggctggta ccacgcgacg aagttcgcgg tcgaaggcat gagcggctcg ctgcgagtcg
     3901 agctccgccc gttcggcatc gacgtcgtcc tgatcgagcc cggcggcatg aacaccgagt
     3961 ggggcgagat tgccgccaac ggcctcatcg ccgcatccgg cagcacggcg tacgccgatc
     4021 aggcccccgg agtcgccaag atgctgcggt cgatagacga aaagccgcgc ccgtcggcgt
     4081 cccctgatcg catcgcgaaa gccgtcaccc gcgccgcgac cgcacgccgt ccaagggtgc
     4141 gctacttgct cggacctggc gccaagccgc tggtcacgct gcagacgatc ctgccggacc
     4201 gggttttcga cgcgatgatg gctcggatct tcggaacacc tcgctaagcg ccgatcaaga
     4261 cagtggaacc gtggttggct gggtcacgtc gaacccccga cagatcacac gggttcggcc
     4321 tggaggaaca gcatgcccgg catctcagta cgcagcggca cagccgcgat agccatcgtg
     4381 accgcaggcg tgctgctcgc cggttgtgcg agcaagagca cggccgctgg ttctggtaac
     4441 cagcctccca agttgctgat cggcaccggc acctcgtccg gaaatgcagc cgccccgatg
     4501 atggccgccg ccggcgtccc cgctcgcggc gcagccattg caccgatgcc catcggcggg
     4561 ttcgggggat tcggcggcta cgtgctctcg ggcacgctgc cgacccagcc gacccacgca
     4621 ccgatctgga cctggcaatc aggcaaggca agcgagtccg acgtcacgaa gctcgcgacc
     4681 gcgctcgggc tgaccggcac gccgacacgt cacgcctacg gctgggacct cacgacgtcg
     4741 gccggtgacc tgcgggtgcg cgacggggac ggcgagcagt ggtcttacaa ccgcgccgac
     4801 gccgtcccgt gccccgcgta tcaaaccgac atcgacaacg ctgacggcgc atccgtcggt
     4861 tacggctgcg cagtttctgc gccgccgccg gtggcaacgc ccggcacgac gacctccggc
     4921 accacagcca tcgtgacccc aatcgacccc gtgcagggac ccgacgagac cgcgaccaag
     4981 gccgctgctg ccagcctcct ctcgacactc ggcgtcaccg gcagcgagca gttcaacgaa
     5041 tcgtcgccag catcgactct gtcggtcgcg ccgcagatcg acggcatgcc gacgcaaggc
     5101 atcgaaacca gcgtggacgt cgatgcgaag ggaattcggg ccgctaccgg ccgtctcgtg
     5161 gcgccgaaag ccggcaccga ctacccgttg cagacagcag cggccgcgtt caagtcgctc
     5221 gccgaccggc caatgccgat gatcgcgcag tactgcggac ccattccggt cgacggcggc
     5281 ccagggctgc cggtgccggc ggccagcgga ccgaacacaa agttcacgct caaccccggt
     5341 gcttcggccg tgcccgcccc taccgtcacc ggaccgaaca cgaagttccc gctaaacccc
     5401 gcagctccgg cttcgcctaa cacggtgatg gtcgtttcag cgccacccgc tcctctgagc
     5461 gccccggccg tcgatagcac cggcccggtc gcggtgccgc ccgtcgagtc ggcgtaccca
     5521 tgcccaacgc cggtgccaca gaaggtgacc ggtgcggtca tcggtctgca ggttcagtac
     5581 gacgccagcg gcgcgacaac cagcgctgac gggtcgacca gtggcggctc caacatcctg
     5641 gtgccgacct ggttcttcac ggtcgagggc tcgacggatc cgctgaccgt gatcgctgtc
     5701 gacccgtcat tcctcggcga ccagacgatc ccgccggtcg gcgcagcatc ggccggctcg
     5761 gcgtccggtg gcttcaccgg atcgggcggc ggatcagccg gctccggaac ggtcggggtc
     5821 ccgcccaccg tcgcatcagc cggacccgcg ctcccacccg cacccgcagc gaagccgtag
     5881 gtcgcggtac ccctatccga ctcgtgatca tgcacaaagt aggtagtcga tcccgccccc
     5941 gtcggctacc tattttgtgc gtaatgatca ccggaggcag tcagaatttg cagcggccca
     6001 accgatcgtg tggactgcgt ccatgccgcg gcttggagca tggacgtcgt cgctgcggcg
     6061 tcgcggaatg tgggggctgt cacggccgcg atgggacgcc gtagcgaaac gttcgatccg
     6121 cgccgccatc gtcattccag ccctgttcgc gttcgctgac aaggttctca aggacccgaa
     6181 catcgcgacc ttcgcgtcca tcggcgcctt ctccgtgctg ctgctcgccg acttcggcgg
     6241 tccgctgcgc cgtcggttca tcgcctatgc ggggctcgcc gtcgtcggtg cggtcttcat
     6301 cacggtcggc acggcgtgct cacgaaccac ctggctcgcc gtcgtcgtcg cagcgatcgc
     6361 ggccgctctc gtgctgttcc tcggaatcct gagcgggtac gtcgccaagg ggtcgaccgc
     6421 gtcgctgctc gtgttgatcc tgcccctcac actgcccgca cacgcgagtg cgatcgggcc
     6481 acggctggag ggttgggcgc tcgcgtcggt cgtcggcacg ctctccgtct tcctcgtgtg
     6541 gcgcgatccg ccgcaggccg gcctaggcac gctggccggc aacgcgctgc accgactcgc
     6601 cgaccacctg cgcgccaccc tcgacggcga acccgtcgag gaaaactcag aggcagcaga
     6661 acaagcagtc gccaagctca aaggtgccta cctggccacc ccattcaggc cagccggcgc
     6721 gacgctgtcc gag
//
LOCUS       DATCBJ010000448         2134 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_376120, whole genome shotgun sequence.
ACCESSION   DATCBJ010000448 DATCBJ010000000
VERSION     DATCBJ010000448.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2134)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2134)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2134
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_376120"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(15..854)
                     /locus_tag="VII50_09055"
     CDS             complement(15..854)
                     /locus_tag="VII50_09055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HEY5051037.1"
                     /translation="MSGDQLLGLLAGAGFGLGVWLLICGVQRRELRPRTASRFSVDKR
                     RVGITVAAVVAAAVISRWPVAALGAGALAWYWPRLVGLSSGDRQTIARLDALATWTES
                     LRHTVAGAIGLEQAIPATAFACAPAIRPHLLRLAGQMQARVPMAVALRSLADDLNDPS
                     ADLVVAALRLSAQLRGPGLRETLSALATSARAELEMRQKVEAGRKTLRRGAAIIAGVT
                     VVFAGGLTLFNGRYLRPYNSLTGQLMLAVVCAVFGVGFGWLGKLSKQPPQGRIYAPPA
                     GAR"
     gene            complement(851..>2134)
                     /locus_tag="VII50_09060"
     CDS             complement(851..>2134)
                     /locus_tag="VII50_09060"
                     /inference="COORDINATES: protein motif:HMM:NF012652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HEY5051038.1"
                     /translation="SAGRVPSAQMYQVRAAAFANEAVAEHMQTLLASGQQAPTPAEEE
                     ALVDAILARMFGAGRLQQMLEDPALADMTDLDINGCDEVFATFTDGRKQRLPSVAETD
                     EELVELVQTLASYVGRNPRPWDAASPQLNLRLSDASRLAATHPSLSGRPVVSVRRSLH
                     PRVFLADLVRFGSVSPELAAFLRAAVRARFNLIIAGGTRAGKTTLLRALVNEIDRTER
                     LIICEDSLEIGLRAHLDLHENVVEMESRLPNSEGVGAVTLAQLVRHTLRMNPDRVIVG
                     EVRGPEALVMLSAMTQGNDGSMSTIHARYARQVFERLATLAASAGDGMDLVTSRVLIS
                     QAIDFVIYCDQVRTGPRSRQRVVSTVLEVTGFDGVVQANEVFRLAGTQAAYTSIALSR
                     QDALADAGWTPDRYDSAPSESYLHAPVSTPMSWSG"
BASE COUNT          367 a          741 c          708 g          318 t
ORIGIN      
        1 caccacgagg gtggtcatcg cgcgcctgcc ggtggtgcgt agatgcggcc ctgcggtggc
       61 tgcttggaga gcttgccgag ccagccgaat ccgacgccga acaccgcgca caccacggcg
      121 agcatcagct ggcccgtcaa cgagttgtag ggccgcagat acctgccgtt gaataacgtc
      181 agtccgccag cgaaaacaac ggtgacgccg gcgatgatgg ccgcgccacg gcgtagcgtt
      241 ttgcggccgg cttcgacctt ttgccgcatc tccagctccg cacgagccga ggtcgcgaga
      301 gccgacagcg tttctcgaag cccaggtccg cgtaactgtg ctgacaaccg aagtgcggcg
      361 accaccagat cggcggaggg gtcgttcaaa tcgtcggcca ggcttcgaag cgccaccgcc
      421 atcggcaccc gagcttgcat ctggccggcg aggcgaagca gatgcggccg gatcgccggc
      481 gcgcacgcga acgcggtcgc cgggatcgcc tgctccaagc cgatcgcgcc ggcaacggtg
      541 tggcgcaggc tttcggtcca ggtcgccaac gcatcaagcc tcgctatcgt ctgccggtcc
      601 cctgagctca ggccgaccaa ccgcggccaa taccacgcaa gcgcgccagc gccgagcgcc
      661 gcgaccggcc agcggctgat caccgccgcg gccacgacgg ccgcaacagt gatcccgacg
      721 cgacgcttgt ccaccgagaa cctcgacgca gtccgcgggc gcagctcgcg acgttgcacg
      781 ccgcagatca acagccacac accgagcccg aacccagccc cggcgagcag gccgagcagt
      841 tggtcgccgc tcatccagac caactcatcg gcgtgctcac gggcgcgtgc aggtacgact
      901 cggacggcgc tgagtcgtat ctgtccgggg tccagcccgc gtcggcgagc gcgtcctggc
      961 gggacaacgc gatgctggtg tacgccgctt gagtaccggc gaggcgaaac acttcgttcg
     1021 cctgcaccac gccgtcgaag ccggtgacct cgagcaccgt cgacaccacc cgctggcgac
     1081 ttcgcgggcc ggtgcgcacc tggtcgcagt agatgacgaa atcgatcgct tgggagatga
     1141 gcacccgcga ggtgaccaga tccatcccgt cgcccgcgga tgcggccagt gtggcgagcc
     1201 gctcgaagac ctgcctggcg tagcgggcat ggatggtgct catcgacccg tcgttgcctt
     1261 gggtcattgc cgacaacatg acgagtgctt ccggaccgcg aacctcaccg acgatgaccc
     1321 ggtcgggatt catccgcagc gtgtgtcgca ccaactgggc aagcgtgacc gcgccgacgc
     1381 cttcagagtt cggcaacctc gactccattt cgaccacgtt ctcgtgcaag tccaagtgcg
     1441 cgcgcaggcc gatctcaaga gagtcttcgc agatgatcaa ccgctcggta cggtcgatct
     1501 cgttgaccag ggcgcgcagc aacgtcgtct taccggcccg cgtcccgccg gcgatgatga
     1561 ggttgaaccg ggcccgcacc gccgcccgca agaacgcggc aagctcggga ctgacgctgc
     1621 cgaaccggac caggtcggcc aggaacaccc gcgggtgcag cgagcggcgc accgacacca
     1681 ccggccgacc cgacagcgac gggtgggtgg cggcaagccg cgacgcatcg gataggcgca
     1741 ggttgagctg cgggctggcc gcatcccatg gcctggggtt gcggccgacg taggacgcga
     1801 gggtttgcac gagctcgacg agttcctcat cggtctcggc gaccgacggt aggcgttgtt
     1861 tgcggccgtc ggtgaaggtg gcgaacacct cgtcgcagcc gttgatgtcg aggtccgtca
     1921 tgtcggcaag ggcggggtcc tcgagcatct gttgcagtcg ccctgcgccg aacatcctgg
     1981 ccaagatcgc gtcgaccagc gcttcttcct ccgctggtgt cggcgcctgc tggcccgagg
     2041 cgagcaaggt ctgcatgtgc tcggcgacgg cctcgttcgc gaacgccgcc gcacgcacct
     2101 ggtacatctg cgccgagggc acgcgcccgg cgct
//
LOCUS       DATCBJ010000449         4347 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_334386, whole genome shotgun sequence.
ACCESSION   DATCBJ010000449 DATCBJ010000000
VERSION     DATCBJ010000449.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 4347)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4347)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4347
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_334386"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..463
                     /locus_tag="VII50_09065"
     CDS             <1..463
                     /locus_tag="VII50_09065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5051039.1"
                     /translation="GFITNVCSAITVADAQATVQPAIDAIGFDPNNQDPGGIFACTID
                     AADQAVTVTVEPEDVAMANYESDVAAENVPAMPLSGVGDVAVWTQVSNNPPDFYAHKG
                     PVTCEVKTTDTQVLNLTYDPTVVDGGVTAASAAAYAVKLGTLCTDVFAAIG"
     gene            complement(482..949)
                     /locus_tag="VII50_09070"
     CDS             complement(482..949)
                     /locus_tag="VII50_09070"
                     /inference="COORDINATES: protein motif:HMM:NF012479.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HEY5051040.1"
                     /translation="MTTLQGRHTTFGELAAGRVALVVNVASLCGKTPQYAKLEQLHEE
                     LADRGFTILGFPCNQFGEQEPGTAAEIAEFCSDTYGVTFPMSDKIEVNGAHRDPIYDV
                     LAQTPYAGAEAADISWNFEKFVVSAQGEVVGRFLPKTQPDDPAVLACIEAALA"
     gene            complement(1026..2423)
                     /locus_tag="VII50_09075"
     CDS             complement(1026..2423)
                     /locus_tag="VII50_09075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HEY5051041.1"
                     /translation="MVSDDRADQPFDLRDVLARRGDERYDLHRQYLNPQLPRMLHAIG
                     FDKVYARAQGAYLYDRDGAEYLDMLAGFGVFALGRHHPVVRQAIHDVIDADLADLTQF
                     DAPPLAGPLAEALLAKVPHLDRVYFGNSGTEAVEAALKFARYATGRRRIVYCDHAFHG
                     LTAGSLSVNGSNAFRKGFGPMLPDTAIPFGDLQALERELRRGDVAGFLIEPIQGKGVA
                     VSPPGFLPAAQELLRNHKALLIADEVQCGVGRTGDFFAFQHEDVEPDLIATAKALSGG
                     YIPIGATIGRDWIFAKVYSSMDRVLVHDSTFGSNAQAMAAGLATLHVLEQENVIDNAR
                     KIGELLQSRLADLVDRYELLHEVRGRGLMIGIEFGRPTSWKLRGSWTALQTARKGLFA
                     QTIVHALFHQHRILTQVSGDHLEVIKLIPPLIIGEREVDLFVDAFVDVMDEAHRGSGL
                     VWDFGKTLVKQARAK"
     gene            2607..3689
                     /locus_tag="VII50_09080"
     CDS             2607..3689
                     /locus_tag="VII50_09080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019993176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HEY5051042.1"
                     /translation="MDELRATDKGEQAALDGVEPPAVLARARALVVPALRAAVDRLHG
                     EHMRRIAAYHLGWLDADGRPVPGHRVGGKAIRSTLAVLSAEAGGGTAGSGVPAAVAVE
                     LVHNFSLVHDDIMDRDVERRHRPTAWVVFGEGQAILAGNAMLTAAVDVLVRDGAAGQR
                     ALPSLLIAVEGLIDGQSADLDLGTRYVGTDRDVDLDDVLAMEAGKTAALLACSSSIGA
                     LAAGADAGVVDALASYGHELGMAFQLVDDILGITGDAAATGKSSSSDVRAGKRSAPIV
                     AALTSGTSASEQLARMLAGGPLTADDDVTHATKLIDEAGGLEWAAHEADRRLALALGH
                     LENLSAPTGTTSDLAALARYVVERDR"
     gene            3708..>4347
                     /locus_tag="VII50_09085"
     CDS             3708..>4347
                     /locus_tag="VII50_09085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="squalene--hopene cyclase"
                     /protein_id="HEY5051043.1"
                     /translation="MTALTYTDLTDAVGLAVKRAQAHLVGRQDGAGWWKGELRTNVTM
                     DAEDLLLRQFLGILKPDETAEAARWIRSQQRADGTWANFERGPGDVSTTIEAYAALRL
                     AGDGVDAPHMAAARAFILAGGGIEASRVFTRIWLALFGEWSWDDLPSMPPELVLLPKW
                     FPLNVYDWGCWARQTVVAITVVATLRPVRPLPFTVAELRTGVRPAPAKTGPVA"
BASE COUNT          731 a         1412 c         1471 g          733 t
ORIGIN      
        1 cggcttcatc actaacgtct gttcggcgat tacagtcgcc gatgcgcagg ccacggtgca
       61 gccggcgatc gatgcgatcg gcttcgaccc caacaaccaa gacccgggcg gcatcttcgc
      121 gtgcacgatc gacgcagcgg accaggcggt gaccgtgacg gtcgagcctg aggatgtcgc
      181 gatggcgaac tacgagtccg atgtcgcggc ggagaacgtc cccgcgatgc cgttatctgg
      241 cgtcggcgac gtcgctgtgt ggacccaggt ctcgaacaac ccgcccgatt tctatgcgca
      301 caagggcccc gtcacctgtg aagtgaaaac caccgacacc caggtgctca acctaaccta
      361 cgaccccacc gtcgttgatg ggggagtaac cgctgcgagt gcggccgcgt atgcggtcaa
      421 gctcggcaca ttgtgcacgg acgtgttcgc ggcaatcggc tgacgcagcc ggcaggccag
      481 gctatgcgag ggcggcctcg atgcaggcga gcacggccgg gtcatcgggc tgggtcttcg
      541 gcaggaagcg gccgacaacc tcgccttgcg cagagacgac gaacttctcg aagttccagc
      601 tgatgtcggc agcctcggcg cccgcgtaag gcgtttgagc aagtacgtca tagatgggat
      661 cgcggtgggc gccgttgact tcgatcttgt ccgacatcgg gaaggtgacg ccataggtgt
      721 cggagcaaaa ctcggcgatt tcggcggctg tcccgggctc ttgctcgccg aactggttgc
      781 acgggaagcc gaggatcgtg aaacctcgat ctgctagctc ctcgtgcagt tgttcgagct
      841 tggcgtactg cggagtcttc ccacacaggc tggcgacgtt gaccaccaac gcgacccggc
      901 cagcggccag ttcgccgaac gtcgtatgcc tgccttggag tgtggtcact ggtagatcga
      961 gtaagtcact catgcccagg tgcagcgtct gccccgccgg ccgcattccc gcgcagcgac
     1021 actcactact tcgcgcgggc ctgcttgacc agggtcttgc cgaagtccca cacgagtccc
     1081 gatccgcggt gtgcctcgtc catcacgtcg acgaacgcgt cgacgaacag gtcgacctcg
     1141 cgttcgccga tgatgagcgg cgggatcagc ttgatcactt ccagatgatc gccggagacc
     1201 tgggtaagga tccgatgctg gtggaacaag gcgtgcacga tcgtttgcgc aaacaggccc
     1261 ttgcgggcgg tctgcaacgc agtccatgaa ccgcgcagct tccacgacgt cggacggccg
     1321 aactcaatac cgatcatgag gccccggccg cggacctcat gcagaagctc gtagcgatcg
     1381 acgagatcgg cgagccggga ttgcaaaagc tcgccgatct tgcgcgcgtt gtcgatgacg
     1441 ttctcttgct cgagaacgtg cagggtggcc aggcccgcgg ccatagcctg cgcgttcgag
     1501 ccgaacgtcg agtcgtggac caggacgcgg tccatcgacg agtagacctt ggcaaagatc
     1561 cagtcgcgac cgatcgtggc gccgatcggt atgtagccgc ccgacagtgc cttggccgtg
     1621 gcgatcaggt cgggttcgac gtcctcgtgc tggaatgcga agaagtcgcc agtgcggccg
     1681 actccgcact gcacctcgtc ggcgatcagt agcgccttgt gattgcggag cagttcctgc
     1741 gcagcgggca ggaagccggg cggtgagacc gcgacgcctt tgccctggat cggctcgatc
     1801 aggaagccgg cgacgtcgcc acggcggagt tcgcgttcga gggcttgcag gtcgccgaac
     1861 gggatcgccg tgtcgggcag catcggcccg aagcccttgc ggaacgcatt cgatccgttg
     1921 accgacagcg atcccgccgt aaggccgtga aaggcatggt cgcagtagac gatgcggcgg
     1981 cgcccggtcg cgtagcgagc gaacttcaag gcggcctcga cagcctcggt gccgctgttg
     2041 ccgaagtaga cccggtcgag gtgcggcacc ttggcgagca gcgcctcggc gagtgggccg
     2101 gccagcgggg gagcgtcgaa ctgggtgaga tcggcgaggt ccgcgtcgat gacgtcgtgg
     2161 atggcctgcc ggacgactgg gtgatgccgc ccgagggcga acaccccgaa gcccgcgagc
     2221 atgtcgagat actccgcgcc atctctgtca tagaggtagg caccctgcgc cctggcgtag
     2281 accttgtcga agccgatggc gtgcagcatt cgcggcagtt gcggattcag atactgccgg
     2341 tgcaggtcgt agcgctcgtc cccgcgccgc gcgagcacgt cccgaagatc gaatggctgg
     2401 tcggctcgat catccgaaac catcggtgag cccacctcct cgcacaatcg ggcctcggaa
     2461 cgcaccgtaa cccatcgctg ggagcacacg gtggatcgca gcacactgag atcggcggcg
     2521 aaacggcaca atctgatata cagcggttgg acgcaactgt ttactgggca ggtacttacc
     2581 cgttctagac cctacggcgg gcgacgatgg atgagcttcg cgctaccgac aagggcgagc
     2641 aagccgccct tgacggtgtc gagccacctg ccgtcctcgc tcgggctcgg gctctcgtcg
     2701 tccccgctct gcgcgccgcc gtcgaccgcc tccacggcga gcacatgcgc aggatcgctg
     2761 cctatcactt gggctggctg gacgccgacg ggcggccggt cccaggccat cgcgtcggcg
     2821 ggaaggcgat ccggtcgacg ctggcggtgc tctccgccga agcgggcggc gggaccgctg
     2881 gcagcggcgt ccctgcggct gtcgcggtcg agctggtaca caacttctcg ttggtgcacg
     2941 acgacatcat ggatcgcgac gtcgagcgcc gacaccggcc gaccgcatgg gtggtcttcg
     3001 gcgagggaca ggccatcctg gcgggtaacg cgatgctcac cgcagcagtc gacgtcctcg
     3061 tccgcgatgg tgccgcgggg cagcgggccc tgcccagcct gctcatcgcg gtggagggac
     3121 tcatcgacgg gcagtcagcc gatctcgatc tgggcacccg atatgtaggc accgaccgcg
     3181 atgtcgacct cgacgacgtg ctcgcgatgg aggcgggcaa gacggccgcg ctgctcgcgt
     3241 gttcctcctc gatcggggcg ctggccgccg gcgctgacgc aggcgtcgtc gacgccctcg
     3301 cctcctacgg tcacgagctc ggaatggcct tccagctcgt cgacgacatc ctcggaatca
     3361 ccggggacgc tgctgcgact ggtaagtcgt catcgtccga cgtccgggcc ggcaagcgga
     3421 gcgcgcccat cgttgcggca ctgacgtcgg ggacgtcggc atccgagcag ctggcccgga
     3481 tgctggccgg cggtccgctc accgccgacg acgacgtgac ccacgcgacc aagctcatcg
     3541 acgaagccgg cggtcttgag tgggcggccc acgaggccga ccgaaggctc gcgctggcgc
     3601 ttggtcacct cgaaaacttg tccgccccga cgggaacaac ttcggacctg gcggcgctgg
     3661 ctcgctatgt cgtcgaacgt gatcgttagt cgaggagggc agacctcgtg accgcgctga
     3721 cgtacaccga cttgaccgac gcggtcggtt tggcggtgaa acgcgcgcaa gcccaccttg
     3781 tgggccgtca ggatggcgcc ggctggtgga aaggcgagct gcgcaccaac gtcacgatgg
     3841 atgcagaaga cttgctactg cggcagttcc tcggcatctt gaagcccgac gagaccgccg
     3901 aagctgcgcg ctggatccgt tcccagcagc gcgccgacgg cacctgggcc aactttgagc
     3961 gcggacccgg cgatgtctcg acgactatcg aggcttatgc cgcgcttcgc ttggccggcg
     4021 acggcgttga cgcgccgcac atggcggcgg cccgcgcctt catcctcgcc ggcggcggca
     4081 tcgaggcgag ccgggtgttc acccggatct ggctggcgct gttcggcgaa tggtcatggg
     4141 acgatctccc gtcgatgcct cctgagctgg tgctgctccc gaagtggttc cctctcaacg
     4201 tgtacgactg gggctgctgg gctcggcaga ccgtggtggc gatcaccgtg gtggcaaccc
     4261 ttcgaccagt tcgtccgctg ccattcacgg tcgctgagct gcgcaccggc gtgcggcccg
     4321 cgccggccaa gaccggtccg gtcgcga
//
LOCUS       DATCBJ010000450         2729 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_332678, whole genome shotgun sequence.
ACCESSION   DATCBJ010000450 DATCBJ010000000
VERSION     DATCBJ010000450.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2729)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2729)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2729
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_332678"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..594
                     /locus_tag="VII50_09090"
     CDS             <1..594
                     /locus_tag="VII50_09090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018531116.1"
                     /note="23S rRNA m2A2503 methyltransferase; methylates the
                     C2 position of the A2530 nucleotide in 23S rRNA; may be
                     involved in antibiotic resistance; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HEY5051044.1"
                     /translation="EPLANYNAVLGAVRRLTDPAPDGLGISQRGITVSTVGLVPAIDK
                     LTAEGLNVTLAVSLHAPDDELRDTLVPINTRWKVAEVLDAAARYVEATKRRVSIEYAL
                     IRDINDQAWRADMLGQLLRERLGHLVHVNLIPLNPTPGSIWTASRSKDEKAFVRGLQN
                     QGVTVTVRDTRGREIEGACGQLATDTQPTASSSAEGE"
     gene            597..881
                     /locus_tag="VII50_09095"
     CDS             597..881
                     /locus_tag="VII50_09095"
                     /inference="COORDINATES: protein motif:HMM:TIGR03544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HEY5051045.1"
                     /translation="MPPFEPLPTERAGLIARIKNVAFTPTRFRRDYDERHVDDFLDAV
                     IASLAGSSVPFTPPQIRERELRQVGFKGGYDIEQVDDFRALIAEAVQLLH"
     gene            943..1350
                     /locus_tag="VII50_09100"
     CDS             943..1350
                     /locus_tag="VII50_09100"
                     /inference="COORDINATES: protein motif:HMM:NF019013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ester cyclase"
                     /protein_id="HEY5051046.1"
                     /translation="MDVSALMRRTYDLINAGDIDGFVDLLADDFVEHEVTPGLAATKD
                     GVKQFFTMYVAAFPDLRFTTDDILPSGDKGVARVTITGTNTGDFMGMPATGKSVSVQA
                     IDIIRFGDDGLAHEHWGVVDVMAMMQQLGAVPS"
     gene            complement(1433..>2729)
                     /locus_tag="VII50_09105"
     CDS             complement(1433..>2729)
                     /locus_tag="VII50_09105"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEY5051047.1"
                     /translation="RDVDVAIVGAGFTGLWTAYYLLEHDPTLRVVLLEKEIAGFGASG
                     RNGGWCSALFPASMDKIAAQSSRGAALALRRAMRNSVDEVGAVTTGNGIDCDYTKGGT
                     LSLIRSRAQAGRARDKLEHAREWGETDEDLRLMDAAETSARLNATGVLGGLFTPHCAA
                     IHPAKLVRGLAELVERRGATIHEGTAVTAVSPHRVDTRHGVVRADIVVRATEGYTPTI
                     RGHHRALAPIYSLMMATEPLPTSFWDDAGLAHRETFNDLRHLIIYGQRTSDDRLAFGG
                     RGAPYHFGSRVEPRFDREPNVFEALQRIVAELFPALSGAAVTHTWGGPLGVPRDWFAS
                     VGLDVATGLASAGGYVGDGVATSNLAGRTLADLITGRDSELTALPWVNHHSRQWEPEP
                     LRWLGINGSLAIVKSADDKESRTSRPSRRARLVDRLTAG"
BASE COUNT          486 a          885 c          872 g          486 t
ORIGIN      
        1 gagccgcttg ccaactacaa cgcggtactc ggtgcggtcc gccgtctgac tgatcctgcg
       61 cccgacggcc tgggcatctc acagcgcggg atcaccgtgt cgaccgtcgg tctggtgccg
      121 gccatcgaca agctcacagc cgaaggcctc aacgtcacgc tggccgtgtc cttgcacgca
      181 ccggacgacg agttgcgcga cacgttggtc ccgatcaaca cgcgttggaa agtcgctgag
      241 gtgctcgacg cggcggcccg ctacgtcgag gcgacaaagc ggcgggtgtc gatcgagtac
      301 gcattgatcc gcgacatcaa cgatcaggca tggcgggccg acatgctcgg tcagttgctg
      361 cgagaacgac tcggccacct cgtgcacgtc aatctgattc cgctcaatcc cacgccaggc
      421 tcaatatgga cggcgtcgcg cagcaaggac gagaaggctt tcgttcgtgg gctgcagaat
      481 cagggcgtga cggtaacggt tcgcgatacg cgcggacgcg aaatcgaagg tgcgtgcggg
      541 cagctcgcca ccgacaccca gccgaccgct tcgtcgtccg ctgaaggcga gtgaccatgc
      601 cgccgttcga gccattgccc acagagcgcg ccgggttgat cgcgcgaatc aagaacgtgg
      661 cgttcacgcc gacgcggttt cgccgcgact acgacgagcg ccatgtcgac gatttcctcg
      721 acgccgtgat cgcgagtctc gccgggtcct ctgtgccgtt cacaccgccg caaattcgcg
      781 agcgagaact gcgccaggtc gggttcaagg gtgggtatga catcgaacag gttgacgact
      841 ttcgcgctct catcgccgag gccgtccagc tcttgcacta gtcactgacg tgggccagcc
      901 tgtgctgacg acgctcaccc cacccagcca ggaggattct gcatggatgt ctcggctttg
      961 atgcggcgca cctacgatct gatcaacgca ggtgacatcg atggtttcgt cgacttgctg
     1021 gcggacgact tcgtcgagca cgaggtgacc cccgggcttg ctgcgaccaa ggacggagtc
     1081 aagcagttct tcacgatgta cgtagcggca ttccccgact tgcggttcac taccgacgac
     1141 attctgccga gcggtgacaa gggcgtcgcg cgtgtgacaa tcacggggac taacacgggc
     1201 gatttcatgg gcatgccggc caccggcaag agcgtgagcg tgcaggcgat cgacataatc
     1261 cgtttcggtg acgatggcct cgcgcacgag cactggggtg tcgtcgatgt gatggcgatg
     1321 atgcagcaac ttggtgcggt gcccagctag gccgtaacgc ttcctccaac ctgcccacgt
     1381 agctttctgg atgccgatgc aaagtccgcg cgcagtcgtt gtcgtgacgt catcacccag
     1441 cggttagtcg gtcgacaagc cgtgcgcgtc gagatggccg gctggtgcgc gactccttgt
     1501 cgtcggccga cttcacgatg gccaatgaac cgttgatgcc gagccaccgc aacggttcgg
     1561 gctcccactg ccggctgtga tggttgaccc acggcagcgc ggtgagctcg ctatcgcggc
     1621 cggtgatcag atcggccagc gtgcggcctg cgagattcga cgtcgcgacg ccatcaccca
     1681 cgtagccgcc ggccgatgcg agacctgtcg caacgtcgag tccaaccgat gcaaaccaat
     1741 cacgcgggac gccaagtggt ccgccccagg tgtgagtgac ggccgcgccg gacaacgcag
     1801 ggaacaactc ggcgacgatg cgttgtaacg cttcgaacac gttcggctca cggtcgaacc
     1861 ggggctcgac gcgagacccg aaatggtaag gcgcaccgcg tccgccgaac gcgagtcgat
     1921 cgtcggacgt gcgctggccg tagatgatga gatggcgaag gtcgttgaac gtctcgcgat
     1981 gggctagccc cgcgtcgtcc cagaacgatg tcggtagggg ctcggtggcc atcatcaacg
     2041 aatagatcgg ggccaacgcc cgatggtgcc cgcggatcgt cggcgtgtag ccctcggttg
     2101 ctcgaacaac gatgtccgcg cgcacgacgc catgcctggt gtcgacgcga tgcggtgaga
     2161 ccgcggtgac cgccgtccct tcgtggattg tcgcgccgcg tcgctcgact agctccgcga
     2221 ggccacgcac gagtttcgcc ggatggatag ccgcgcagtg cggcgtgaac agtccgccga
     2281 gcacgccggt ggcgttcagc cgtgccgacg tctccgcggc gtccatgagg cgcaggtcct
     2341 cgtctgtctc gccccactcc cgcgcgtgct caagcttgtc gcgcgctctg ccagcctgtg
     2401 cgcggctgcg gatcagcgag agcgttccgc ctttggtgta gtcgcagtcg atcccgttgc
     2461 ctgttgtcac ggcaccgacc tcgtcgacgc tgttccgcat cgcccggcgc agtgccagcg
     2521 cggcacctcg cgacgactgc gccgcgatct tgtccatcga tgctggaaac aatgcgctgc
     2581 accaaccgcc gttgcgaccc gatgccccga agccggctat ttccttttcc aacagcacaa
     2641 ctcgaagcgt cgggtcatgc tcgaggaggt agtacgcagt ccacaggccg gtgaatccag
     2701 cacccacgat cgcgacatcg acgtcacgg
//
LOCUS       DATCBJ010000451         3963 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_41206, whole genome shotgun sequence.
ACCESSION   DATCBJ010000451 DATCBJ010000000
VERSION     DATCBJ010000451.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 3963)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3963)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3963
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_41206"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(159..1517)
                     /locus_tag="VII50_09110"
     CDS             complement(159..1517)
                     /locus_tag="VII50_09110"
                     /inference="COORDINATES: protein motif:HMM:NF025585.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4331 domain-containing protein"
                     /protein_id="HEY5051048.1"
                     /translation="MSSHREAPEISKDPVADSSDLYAFVSPDKPGYATIIANYVPLQA
                     PDGGPNFFEFGDDVLYEIHISNRGDAQADITYGFRFHTVVFNKKTFLYNTGQITSVFD
                     PHFNRRQYYTVTRTTRGGNTVQLGANLEVPPCNIGPRSTPNYEHLAAQCVHGIGNRTF
                     FAGQRADAFHVDLGSVFDLLDLRPFQNLHLIPTPAGTGVNSLTDLNVHSIAMRVPLSD
                     LTVDGKTPTDPASLKSVVGIWTTASRQRTHVYEGPGATAQNLGGWEQVSRLGNPLINE
                     VVIPMGSKDDWNHSQPSNDSDYAASFLHPEVAGLLPVLYPGVFKNLAAYTKPRADLVA
                     ILGTGIPSGIIPGFQNYTGKVVADMLRLNLAIPATSFAKASPAGLAGGDPAGFPNGRR
                     VTDDVVTIELRAIAGLLIPLVDKSFKPDAATSLIDDGSTMTPTQQVFPYLNTPHDGYS
                     AA"
     gene            complement(1599..1874)
                     /locus_tag="VII50_09115"
     CDS             complement(1599..1874)
                     /locus_tag="VII50_09115"
                     /inference="COORDINATES: protein motif:HMM:TIGR01409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocation signal
                     domain-containing protein"
                     /protein_id="HEY5051049.1"
                     /translation="MAEHSRRAFIRGAGVTAAAATVAVMVPGVASAASKTPSGSPADD
                     GSPAPDGSVVAFVKDAKAGSITVMRGEREVQITDKALARKLAKLTQL"
     gene            2010..2660
                     /locus_tag="VII50_09120"
     CDS             2010..2660
                     /locus_tag="VII50_09120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5051050.1"
                     /translation="MTDWTTTGASLATALGTLVLGVATFASVRSANRAARTAERALME
                     GLRPLLVASRLQDPPEKIGFVDQHWVRVDGGHGSVEVTDAVIYLTIALRNVGRGMAVL
                     HGWFATRGMVASSDRPDHAPVDDFRRLTRDLYIPPGDLGFWQGALRDPNEPVFEEVSV
                     TAKAAEPVTIELLYGDHEGGQRAISRFSLIPHDADGNQVWLAVVSRHWHLDRRAPR"
     gene            complement(2664..3875)
                     /locus_tag="VII50_09125"
     CDS             complement(2664..3875)
                     /locus_tag="VII50_09125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF349 domain-containing protein"
                     /protein_id="HEY5051051.1"
                     /translation="MGTSTAWGRIAEDGTVYVRTADEAERVVGSWQAGTPEDGLAHFV
                     RRYDDLATEVGLLETRLVSGAASASSTVVAVTKLRASLPTANAVGDLTTLDVRLCTLL
                     ATAEAKRADERAAKAAATTKAVDAKKALADEAEKLAQSTQWKPAGDRLRAIVDEWKEI
                     KGVDRKVDTELWKRVSAARAEFGRRRGAHFATLDEQRKEAVAYKEKLATEAESLAQSS
                     EWTPTANRYKSLMVQWKAAARASKDVDEALWQRFRAAQDTFFTRRTAALEEREAEFRT
                     NQEAKESLLTEAESIDPSADLPAAQAKLRDIQTRWTATGRAPREVGVEFDRRLDAVAE
                     RLKAASDDRWRETSLSSSPLVIRLRESVEKLEKKIDRAKQAGKASEVSELETQLATQR
                     EWLAQGERPAR"
BASE COUNT          593 a         1254 c         1354 g          762 t
ORIGIN      
        1 atttgtacag cacgagcgca ccgaagtcgt caccgatatc aagcaccgtc ggtccctgcc
       61 cggccacatg gttgtggccg ggcaggtgac cctcgtgcgc atgaccgtgg tcgtgactgt
      121 gactgggacc gtggtcgcga tgggcgtggc cggtcatgct aggccgcgct gtatccgtcg
      181 tgcggcgtgt tgaggtacgg gaacacttgc tgcgtcggcg tcatcgtcga accgtcgtcg
      241 atcaggctgg tcgcggcgtc cggcttgaac gacttgtcga cgagcgggat cagcagaccc
      301 gcgatcgccc gcagctcgat ggttaccaca tcgtcggtca cccgacgacc gttcgggaat
      361 cctgccgggt ccccgccggc gagaccggcc gggctggcct tggcgaaaga cgtggccggg
      421 atcgcgaggt tcaggcgcag catgtccgcg acaaccttgc cggtgtagtt ctggaaaccg
      481 gggatgatcc ccgacgggat accggtaccc aagatcgcga ccaggtcggc gcgcggcttg
      541 gtgtaggcag cgaggttctt gaagacgccg gggtagagaa ccggaagaag gcccgcaacc
      601 tcggggtgta agaacgacgc cgcgtagtcc gagtcgttcg aaggctggct gtggttccag
      661 tcgtccttcg aacccatcgg gatgacgacc tcgttgatca gcgggttgcc gagacgggag
      721 acctgctccc acccgccgag gttctgtgct gttgcaccgg ggccttcgta gacgtgggtg
      781 cgctggcggc tcgccgtcgt ccagatgccg acgaccgact tcaggcttgc cgggtcggtg
      841 ggcgtcttgc cgtcgacggt gaggtcactg agcggaaccc gcatcgcgat gctgtgcacg
      901 ttcaagtcgg tcagcgagtt cacgccggtg ccggccgggg tcgggatcag gtgcaagttc
      961 tggaacggac gcaagtcgag caggtcgaac accgagccta ggtcgacgtg gaacgcgtcg
     1021 gcgcgctgac cggcaaagaa cgtccggttg ccgatgccgt gcacgcactg cgcagcgagg
     1081 tgctcgtagt tcggggtgga gcgcggtccg atgttgcacg gcggtacctc gaggttcgcg
     1141 ccgagctgca cggtgttgcc gccccgcgtc gtgcgcgtca cggtgtagta ctgccgacgg
     1201 ttgaagtgtg ggtcgaacac cgaggtgatc tggccggtgt tgtagaggaa cgtcttcttg
     1261 ttaaacacga ctgtgtggaa gcggaagccg taggtgatgt cggcctgggc gtcgccacgg
     1321 ttcgagatgt gaatctcgta cagaacgtcg tcgccgaact cgaagaagtt cgggccgccg
     1381 tcgggggctt gcagtggaac gtagttggcg atgatcgtcg cgtaaccggg cttgtccggg
     1441 ctgacgaacg cgtagaggtc tgaactgtct gcgaccgggt ccttggagat ctccggcgct
     1501 tcacggtgag aagacatgcg acaccttctg tgctcgcggt ttatgggtga gtttgaagcg
     1561 acgaacgggg tgagctcgcg ggctgcgaac tcgagctctc agagctgggt gagcttggcc
     1621 agcttgcggg ccaacgcctt gtcggtgatc tgcacttcgc gctcaccgcg catcacggtg
     1681 atgctgcccg ccttcgcgtc cttgacgaac gcgacgacag aaccgtcggg cgccggactg
     1741 ccatcatcgg cgggggagcc ggacggcgtc ttcgaggcgg cgcttgccac ccctgggacc
     1801 atcacggcca cggtggccgc tgcggcggtg acgcccgcgc cgcggatgaa ggcccttctg
     1861 ctgtgctctg ccatggtgat gctccttcga aagcgttctg ggcgagtcat gccggcccgc
     1921 ctgtggtgac tcggactgtc ttcgttgcca ccaggcgcgt tggttcactc ggcctattcg
     1981 cgtctccgtt cagcccataa ggtcagcggg tgaccgactg gacgacgacc ggtgcctcgc
     2041 ttgccaccgc gctcggcacc ctcgtgcttg gcgtcgccac gtttgcctct gtgcgatcgg
     2101 caaatcgagc agcgcggacg gcggagcgag ccctcatgga ggggctgcgt ccgctcctcg
     2161 ttgcgtcacg gctgcaggat ccgccggaga agattgggtt cgtcgatcag cactgggtgc
     2221 gcgtcgatgg cgggcacggg tcagttgagg tgaccgacgc cgtcatctac ctgacgattg
     2281 cgttgcgcaa cgtcggccgc gggatggcag tcctgcacgg ttggttcgcg actcgtggca
     2341 tggtcgcatc gagcgatcga cccgaccacg cgccggttga cgatttccgc cggctgacgc
     2401 gcgacctcta catcccgccg ggtgatctgg gcttctggca aggtgcgcta cgcgacccga
     2461 acgagcccgt ctttgaggaa gtgagcgtga cggcgaaagc tgccgagccc gtcaccatcg
     2521 agctgcttta cggagatcac gaaggcggcc aacgcgcgat ttctcggttc tcgctgatcc
     2581 cccacgacgc cgacgggaat caggtgtggc tggccgtcgt gtcaaggcat tggcacctcg
     2641 accgccgggc gcctagataa gccttaccgg gcgggacgct cgccttgcgc aagccactca
     2701 cgctgcgtgg cgagctgcgt ctcgagttcg ctgacctccg atgccttccc ggcctgcttc
     2761 gcccggtcga tcttcttctc gagtttctca acggactcac gcaaccggat caccaacggc
     2821 gacgacgaca aggacgtttc gcgccatcgg tcatcggacg cggccttgag gcgctcagca
     2881 accgcgtcga ggcgacgatc gaactcaacg ccgacttcgc gcggcgcacg gccggtcgcc
     2941 gtccaacggg tttgaatgtc gcggagtttt gcttgcgccg caggcagatc cgcggaggga
     3001 tcgatggact cggcctcggt gagcaacgac tccttggctt cctggttggt gcggaactcc
     3061 gcttcccgtt cctcgagcgc ggcggtgcgc cgagtgaaga acgtgtcctg cgccgcgcgg
     3121 aaccgctgcc acagcgcttc gtcgacgtcc ttcgacgccc tcgccgccgc tttccattgc
     3181 accatcaggg acttgtagcg gttcgctgtc ggagtccact ccgacgactg agccaatgac
     3241 tcggcctcag tggcgagctt ctccttgtac gcgacggctt ccttgcgttg ctcgtcaagc
     3301 gtcgcgaagt gcgcgccccg ccgacggccg aactccgcgc gggccgcgga gacccgcttc
     3361 cacaactcgg tgtcgacctt gcggtcgacg cctttgatct ctttccattc gtcgacgatg
     3421 gcgcgcagcc gatcaccggc cggcttccac tgggtacttt gagcgagctt ctctgcttcg
     3481 tcggcgagtg ccttcttggc gtcgactgct ttggtcgtgg cagctgcctt ggccgcccgc
     3541 tcgtcagctc gctttgcctc ggccgtggcc agcagcgtgc agagtcgaac gtctaacgtc
     3601 gtgagatcgc cgaccgcatt ggcggtgggc agcgatgcgc gcagcttggt gacggcgacc
     3661 accgtcgacg acgcggatgc ggcacccgac acgagccggg tctcgagcag gccgacttcg
     3721 gtcgccaggt cgtcgtagcg tcggacgaaa tgcgccagcc cgtcttcggg ggtgccggcc
     3781 tgccacgatc cgaccacgcg ctcggcctca tcggcggtgc ggacgtagac ggtgccgtct
     3841 tcggcgatcc ggccccacgc ggtgctggtg cccatcgtgc tccttgctcg gtcgggtgcg
     3901 tttgtgcgcc cggtgtgccc atcttgccgt tacgaacgtg acgcggtgtg cccgcccgcg
     3961 ccg
//
LOCUS       DATCBJ010000452         2656 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_446563, whole genome shotgun sequence.
ACCESSION   DATCBJ010000452 DATCBJ010000000
VERSION     DATCBJ010000452.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2656)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2656)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2656
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_446563"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..191
                     /locus_tag="VII50_09130"
     CDS             <1..191
                     /locus_tag="VII50_09130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5051052.1"
                     /translation="VFAHSGGYQSAQAFTDGVVPAVKLGALIVALGGVAALLIPRKPV
                     PAPEPESITLGDLEPSAA"
     gene            233..1276
                     /locus_tag="VII50_09135"
     CDS             233..1276
                     /locus_tag="VII50_09135"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HEY5051053.1"
                     /translation="MLDSTDPAAGHSLLLADHTTIHLGGPAKNLVWADNAADLIDAVS
                     QADADGTPVLVLGGGSNLVIADAGFDGLVVRVADRGVAFEEQVGEQVVMTAAAGEAWD
                     NVVALALAQGLAGLECLSGIPGLAGATPIQNVGAYGTEISQLVTGVPVFDRVDRRHLI
                     LSAADCRFGYRTSALKGDRRYLVLGVRIALTRSDTSQPVRYQQLADALGVELGGRAAA
                     LDVRAAVLGLRAAKGMLIDPADPDSVSVGSFFTNPILRDAEVPAGAPRWPTSDGLVKT
                     SAAWLIEQAGFARGYGHGPVGLSNKHTLALVNRGGATTAELVAFAAHIRDGVRTRFGV
                     TLDVEPALVGVAL"
     gene            complement(1281..2510)
                     /locus_tag="VII50_09140"
     CDS             complement(1281..2510)
                     /locus_tag="VII50_09140"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEY5051054.1"
                     /translation="MTDDTPGPSSTSRVSRRIGAIAESATLAVDAKAKALKAAGHPVI
                     GFGAGEPDFPTPPAIVEAAIEAARDPKNHRYTPAAGLPELREAIAVKTARDSGLAIKA
                     ADVLVTNGGKQAVYQAFATLLDPGDEVLLPAPYWTTYPEAIALAGGVTVQVDTDESSG
                     FRATVSQLEAARTSRTKALLFCSPSNPTGAVYRPEHVAEIGRWAVEHAIWVLTDEIYE
                     HLVYGDATFTSMPVAVPELAGTCVVLNGVAKTYAMTGWRVGWMIGPSDVIKAATNLQS
                     HLTSNVANVSQRAALAAVSGDLSAVAMMRDAFDRRRMTMVSMLNDIPGVVCVEPQGAF
                     YAYPSVKAILGLELRGKRPSTSAELAELVLDEAEVAVVPGEAFGTPGYFRLSYALGDD
                     DLVEGVNRLGKLLSEAR"
BASE COUNT          405 a          833 c          961 g          457 t
ORIGIN      
        1 cggtgttcgc gcactccggt ggctaccaga gcgcgcaagc atttaccgac ggcgtggtgc
       61 cggcggtgaa gttgggtgcg ctgatcgtcg cgttgggcgg cgtcgcggca ctgcttatcc
      121 cgcgcaagcc cgtccctgct ccggagcccg agtcgatcac tttgggcgac ttggagccgt
      181 cagcggcgta agtaccacgt gttagccggg tggccggccg tagggttcgt gggtgctcga
      241 ctccacagat ccagcggccg gccactcgtt gttgttggcc gaccacacga cgatccacct
      301 tggtgggccg gcaaagaatc tcgtgtgggc tgacaacgca gctgatctca tcgatgcggt
      361 tagccaggcc gacgccgacg gcactccggt gctggtgcta ggcggcggca gcaacctggt
      421 gatcgccgac gccggcttcg acgggcttgt ggttcgggtg gcagatcgcg gggtcgcgtt
      481 cgaggagcaa gtcggcgagc aggtcgtcat gaccgccgcg gccggtgagg cgtgggacaa
      541 cgtcgtcgcg ttggcgctag cgcaaggact cgcaggtctc gaatgcctgt cgggaattcc
      601 tggcctcgcg ggcgcgacac cgattcagaa tgtcggcgcg tacgggacgg agatctcgca
      661 gctcgtgacc ggggtccccg tcttcgaccg ggtcgatcgc cgccatctca tcctgtctgc
      721 ggccgactgc cggttcgggt accgcaccag cgcactcaag ggcgatcgcc ggtacctggt
      781 gctcggcgta cgaatcgcgc tgacacggag tgacacatcg caaccggtcc gctatcagca
      841 gctcgcggac gccctcggtg ttgagctcgg cgggcgcgcc gcagcactcg acgtccgcgc
      901 agctgttctt ggcctacggg cggcgaaggg aatgctgatc gatccggccg accctgactc
      961 ggtgagcgtg ggttcgttct tcaccaatcc aatcctgcgg gacgccgagg tgccggccgg
     1021 cgctccccgg tggccgacca gtgacgggct ggtgaagacg tcggcggcct ggctcatcga
     1081 acaggccggt ttcgctcggg gctacgggca cgggccggtc gggttgtcga acaagcacac
     1141 gctcgcgctg gtgaaccgcg ggggcgcaac gactgcggag ctggtggcgt tcgccgcgca
     1201 catccgcgac ggtgtgcgga ctcggttcgg cgtgaccctc gacgttgaac cggcgctggt
     1261 cggcgtcgcg ctgtaggaag ttatcgcgct tcggacagca gtttgcccag ccggttgacg
     1321 ccctcgacga ggtcgtcgtc accaagcgcg taggacagcc ggaagtagcc cggcgtgccg
     1381 aaggcttcac cagggacgac ggcaacctct gcctcgtcga gcacgagttc ggcgagctcg
     1441 gcagacgtcg aaggacgctt gccgcgaagc tcgaggccga ggatcgcctt cactgacgga
     1501 taggcataga acgcgccttg cggttcgacg cacacgacac ccgggatgtc gttcagcatc
     1561 gacaccatcg tcatgcgccg gcgatcgaac gcgtcgcgca tcatcgcgac cgcggagagg
     1621 tcgcccgaga cggccgcgag tgcggcgcgt tgtgaaacgt tggcgacgtt ggaggtcagg
     1681 tgcgactgca ggttggtcgc ggccttgatg acgtcggacg ggccgatcat ccagcccacc
     1741 cgccagcccg tcatcgcgta ggtcttggcg acaccgttca gcacgacaca cgtaccagca
     1801 agttcgggga cggcgacggg catggaggtg aacgtcgcgt cgccgtagac gaggtgctcg
     1861 tagatctcat cggtgagcac ccagatggcg tgctcgaccg cccaacgacc gatctcggca
     1921 acgtgctcgg gtcgatagac cgcgccggtc gggttggacg gcgagcagaa gagcagagcc
     1981 ttggtgcgcg acgtgcgcgc ggcttcaagc tgacttacgg ttgcgcgaaa tccgctggac
     2041 tcatcggtgt cgacctgcac agtgacgccg ccggcgagcg cgatcgcttc gggatacgtg
     2101 gtccaatacg gcgccgggag cagcacctcg tcgccggggt cgagcaacgt cgcgaatgcc
     2161 tggtagaccg cctgcttgcc gccgttggtc acgagcacat cggcggcctt gattgccaag
     2221 cccgaatcgc gagccgtctt caccgcgatc gcctcacgca gttctggcag gccggcggcg
     2281 ggggtgtagc ggtggttctt cgggtcgcgg gccgcctcga ttgcggcctc gacgatcgcg
     2341 ggcggcgtgg gaaagtcggg ctcgccagct ccgaatccga tgaccgggtg accggcggct
     2401 ttgagggctt tggccttcgc gtcgaccgcg agggtggcgg attcggcgat cgcgccgatt
     2461 cggcgggaga ccctgctggt tgatgagggg ccgggggtgt cgtcggtcat ggtccccatg
     2521 gtcccatgag agctagcgcc gacgcgccgg ggtttcttgg cgcgggagcg cccaggcttg
     2581 gtggcgcggg agcggttcga ccgtgcgtgg cccctccacg tacactgtcg gggctggtcc
     2641 ctggcggtaa acagcg
//
LOCUS       DATCBJ010000453         2647 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_364476, whole genome shotgun sequence.
ACCESSION   DATCBJ010000453 DATCBJ010000000
VERSION     DATCBJ010000453.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2647)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2647)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2647
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_364476"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..513)
                     /locus_tag="VII50_09145"
     CDS             complement(<1..513)
                     /locus_tag="VII50_09145"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY5051055.1"
                     /translation="MSNSTSPALAAVDLVKTYPGARGKARIRALDGLSIDVQAGSVFA
                     LLGPNGAGKSTAVKILATLSRPDSGHASIAGIDVIRDPDGVRHIIGLVSQKSSSDPMA
                     TARENLVLAGRIHGMSSSCARARAADLLSRFALDDAAQRLARTFSGGMARKLDVAIGL
                     MHRPQVLFLDE"
     gene            complement(671..1357)
                     /locus_tag="VII50_09150"
     CDS             complement(671..1357)
                     /locus_tag="VII50_09150"
                     /inference="COORDINATES: protein motif:HMM:NF015513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEY5051056.1"
                     /translation="MARRRMSNPLALAVLACLVERPMHPYEISTTLRSRGKEQSIKLN
                     YGSLYSVVESLEKHGLIRARETTREGRRPERTVYEITDAGAIECEDWLAELISVPTRE
                     FTSLEAGLSLLPLLAPMEAARLLDDRAERLRIEQRVIDAAHAEAASIELPEIFTIEST
                     YRRTMIAAELDFVTALARDIRSGAFKGTQWWQRIHDLRAEGIGFEQLAEDPVGLLGEE
                     AARWIKPASN"
     gene            complement(1434..1910)
                     /locus_tag="VII50_09155"
     CDS             complement(1434..1910)
                     /locus_tag="VII50_09155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5051057.1"
                     /translation="MPPARSTSTLARAVISSAPDPAPAVASSPVQSPSPDWVGVVGAL
                     DRSRDGAFADANANELDAIYVPGSAALATDRETLGGLVGAGQRVRGLQFVLESVRVVA
                     QSPAEVTLTVVDTLAGSDVVDSGGVATHVPGRGVRNWTVVLSAQQDGWRIASITPA"
     gene            complement(2128..>2647)
                     /locus_tag="VII50_09160"
     CDS             complement(2128..>2647)
                     /locus_tag="VII50_09160"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEY5051058.1"
                     /translation="ALRSVVSTTDGLVLVLDYAEGGSLAAVLGVRGRLSTGEVVAIGA
                     PLGQALAEIHARGLTHGDVTPGNIVFDRSGKPLLADLGVANLIGEQAGPAGRTPGLTA
                     SPTRRSPGRCGYSRPGPRPHSSRRSRQGSIPTPPPGPMPRPSPVPCLRRAHQSRFGSH
                     TRAKPSAGRLPR"
BASE COUNT          452 a          921 c          891 g          383 t
ORIGIN      
        1 ctcgtcgagg aagagcacct gcggtcgatg catcaggccg atcgcgacat cgagcttgcg
       61 agccatgccg cccgagaagg tccttgccaa ccgctgcgcc gcgtcgtcga gcgcgaaccg
      121 ggacaacagg tccgcggcgc gagcacgtgc gcaggaactc gacatcccgt ggatgcggcc
      181 ggcgaggacc aagttctcgc gcgccgtcgc catcgggtcg ctcgacgact tctgcgagac
      241 caggccgata atgtgccgca ccccatcggg gtcacgaatg acgtcgatcc cggcgatgct
      301 ggcgtgccct gagtccggcc gcgacagcgt ggcgaggatc ttcacagccg tggacttacc
      361 ggcaccgttc gggccgagca atgcgaagac gctgccggcc tgcacgtcga tggatagccc
      421 gtccagcgca cggatgcgtg cctttccgcg tgcgcctgga taggtcttca cgagatcgac
      481 ggcagcgagt gccggcgagg ttgagttgga catcgaactc cttcatcgac gcttgcgccg
      541 agcgcaagcg ccatatggat gggagtcgag accgcagcgc ctatcgtgtg agtgtgcctc
      601 gggacggacc gcctgcggtc tcaactcggg tgtatggccc ctggccggtg ttgacgcacc
      661 ggccaggggt tcagttactt gctggtttaa tccagcgcgc cgcctcctcc cccaggagcc
      721 cgaccggatc ctcggcgagt tgctcgaagc cgatgccctc ggcgcgcaag tcgtgaatac
      781 gctgccacca ctgcgtgccc ttgaacgcgc ccgatcggat gtcgcgcgca agcgcggtca
      841 caaagtccaa ctcggcggcg atcatcgtgc ggcggtacgt gctctcgatc gtgaagatct
      901 ccggcagttc gatgcttgcc gcctccgcgt gcgcggcgtc gatcacgcgc tgctcgatgc
      961 gcagccgctc ggcgcggtcg tccaagagtc gcgcggcctc catcggggcg agcagcggca
     1021 acagcgacaa gcccgcttcc agcgaagtga actcacgggt cggcaccgag atcagctcag
     1081 cgagccagtc ctcacactcg atcgcgcccg catcggtgat ctcgtagacg gtgcgctccg
     1141 gccggcgccc ctctcgggtg gtttcgcgcg cccggatcaa cccgtgcttc tccagcgatt
     1201 cgacgaccga gtacaacgag ccgtagttga gcttgatgct ctgttccttg ccgcggctgc
     1261 gcagtgtggt cgagatctcg tacggatgca tcggtcgctc gacgagacac gcgagcacgg
     1321 caagagcaag cgggttggac atccgacggc gcgccatccg aactcctctg gttcgactac
     1381 tcgaatcaga gtattagcat caggacagcc ggtgtcaagt aatggcgagc cgctcaggcg
     1441 ggtgtgatcg acgcgatccg ccagccgtct tgctgtgcgc tcaagacgac ggtccagttc
     1501 cgcacacccc gcccggggac atgcgtcgcc actcccccgg aatcgacgac gtcagagccg
     1561 gccaaggtat cgaccacggt cagagtgacc tccgctggcg actgcgcgac gacccgcacc
     1621 gactcgagga cgaactgcag accgcgcacc cgctgaccag cgccgacaag gccacccaac
     1681 gtctcgcggt cggtcgccaa ggcggcggag ccaggcacgt agatggcgtc gagttcgttc
     1741 gcattcgcgt cggcaaaggc gccgtcacgg ctgcggtcga gcgcacccac aacgcctacc
     1801 cagtctggcg acggcgactg cacgggcgac gaggcaactg ccggcgcagg gtctggcgct
     1861 gatgagatca ctgcacgtgc gagcgtggac gtcgagcgtg cgggcggcac gtccgcacgc
     1921 tcgccgacgc gcggcgaccc ctacggcaac ggccgcccag cagggccacc acggatgcgg
     1981 ccgtaaccag gcggctcatc agcacacgac ggccggccga ccagcggtgc cggcccagct
     2041 gaccaccgcg acgaaaacgt gaggccggcc gtgcctccgt atcaggggac gattccgcgg
     2101 cgcgcacccg gtccggcacc gcctcagtca tcgcggcagg cgtcccgccg acggcttggc
     2161 tcgcgtgtgc gagccgaacc gcgattggtg agcacgacgc aaacaaggca cgggcgaagg
     2221 ccgcggcatc gggccgggcg gcggggtcgg gatcgagccc tgcctcgatc gccgcgacga
     2281 gtgcggccgg ggccccgggc gcgagtaacc gcagcgaccg ggcgaccgtc gggtcggact
     2341 cgccgtaagg ccgggcgtcc gcccagccgg acccgcctgc tcgccgatga ggttcgcgac
     2401 gccgagatct gcgagcagcg gcttgcccga cctgtcgaac acgatgttgc ccggggtgac
     2461 atcgccgtgc gtcagaccac gcgcgtggat ctcagccagc gcctgaccca gcggtgcgcc
     2521 gatcgcgacg acctcgccgg tggacaatcg gccgcggacg ccgagcaccg ccgccagact
     2581 gccgccctcg gcgtagtcaa gcacgaggac aagaccgtcg gtcgtcgaga cgacagaccg
     2641 cagcgcg
//
LOCUS       DATCBJ010000454         4068 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_91012, whole genome shotgun sequence.
ACCESSION   DATCBJ010000454 DATCBJ010000000
VERSION     DATCBJ010000454.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 4068)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4068)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4068
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_91012"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..261
                     /locus_tag="VII50_09165"
     CDS             <1..261
                     /locus_tag="VII50_09165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5051059.1"
                     /translation="YVKGGFYNNHYDSAAIDTLITQEEGETDATKRTAELQQIQALAA
                     KDVPTIPIWQGNQVAATGLTVTGVDKTFDPSYIFRMWLIGKS"
     gene            409..1437
                     /locus_tag="VII50_09170"
     CDS             409..1437
                     /locus_tag="VII50_09170"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY5051060.1"
                     /translation="MASSSASLRRYALTRLALVIPMVLILLTVVFLLMRVAPGDPIQA
                     ALGGKLSAAQLEEKRRAAGFTKPLIVQYVEYLKQVLTLNLGTSITDNQAITTVLKVNG
                     AATLELSVTASVIALVIGVPIGLRAGRLRDSAFDAGGRLFGIITYAAPVFFVGLLFQL
                     VFSKWLNWLPNSGQLSPIDQAELPAHTHLSVVDAIWDHNMSAFVDAVKHLAMPSFTLG
                     LLLCGVVIRLIRTNLIQTLKGDYVEAARARGVPEHKVVMRHAFRNALVPVVTVMGLQA
                     ALLLSGAVLTEETFNWPGIGNQLVTYLNNRDYIAVQGIITIFALAVVFISLLIDFVNA
                     AIDPRVRY"
     gene            1437..2501
                     /locus_tag="VII50_09175"
     CDS             1437..2501
                     /locus_tag="VII50_09175"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY5051061.1"
                     /translation="MTAASVAEPGGGSLLDTPPPTGERPRGPFGRLWEQVKSAQGFSK
                     WLLWVGAAITLAFVVLAVFAPLLAPYGFDQYMSHGVRFAKQGSPSGSHWFGTNVRSVD
                     VLSVVIYGSRTAIEVVVLAVVFSLVIGVPLGMISGFYGGWLDRILVLIMDALFAFPYL
                     LLAIIIAFLLQNSVGSGVLTAAIAITVIYVPQYFRVVRGQVLSAREEPYVEAARALGA
                     RPRTIMGRYLFFNVIQSVPVIATLNAADAILTLAGLGFLGFGIQPTQAAEWGYELQRG
                     VADMGAGLWWTSVFPGIAIVSLVTGLTLVGEGLNDVLNPVLRKRSGRRVLLPKRDAAA
                     SATTLPSRQVDNHGRGEELS"
     gene            2498..3526
                     /locus_tag="VII50_09180"
     CDS             2498..3526
                     /locus_tag="VII50_09180"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY5051062.1"
                     /translation="MTAAPVLSVRDLRVWYGTDRGPVRAVDGVSFDVRAGETLGLVGE
                     SGCGKSTLGRGILGLLPSGSVVDGSVRFRDRELVGVKQSDMREMRGAELGLIFQEPMT
                     RLDPLMRISEHFAETLRAHEPELSSTEIDARSIDTLRRMGIPPTRFRHYPHEFSGGMR
                     QRIMIALALVLNPAFVVADEPTTALDVLVEAQILSILHDLKRNFDTSLLLITHNLGIV
                     AEACERVAVMYAGRIVEEGDARQVFSDPSHPYTRELLRSTISLSTTALHYIPGSPPDL
                     VEPPSGCHFHPRCPSAMQVCRSLDPVEVLAPSGQRVSCWLHGPDELLPPGGAAPLQRQ
                     ELASADEA"
     gene            3510..>4068
                     /locus_tag="VII50_09185"
     CDS             3510..>4068
                     /locus_tag="VII50_09185"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY5051063.1"
                     /translation="MPTKPDNVDLDLDATPLDDRPVVLDVRDLRVHFALRGGFFDRLL
                     GRSSGSVKAVDGVSFTLRKGEVLGLVGESGSGKTTTARALLGLAPITGGTVRFDAGPD
                     YGGAVELTGLREPQFRAVRQAIQLVFQDPHASLNPAMDLAAAVAHPLKIHHITKDRAE
                     IDRRVRAAFERVGLAPVDQYLHKYPA"
BASE COUNT          654 a         1307 c         1307 g          800 t
ORIGIN      
        1 tacgtcaagg gcggtttcta caacaaccac tacgacagcg ccgcgatcga caccctcatc
       61 acgcaggagg aaggcgagac cgacgccacc aagcggacgg ccgagctcca gcagatccag
      121 gcgctggcag ccaaggacgt gccgaccatc ccgatctggc agggtaacca ggtggcggcc
      181 accggactca cagtgaccgg cgtcgacaag acgttcgacc cctcgtacat cttccggatg
      241 tggctcatcg gcaagagtta atctcgggcg ctccgccgag ccgacctggc cagctagcga
      301 gggccaggtc ggtcggcggg cggaggtgca tcgacatcgc gggtgcgccg ccggtctacg
      361 gtggcgtacc agcagtcgga ctcgagcctg acgagaggca accgttccat ggcgtcatca
      421 agtgcgtcgc tgcgccggta tgcgctgacc cgactcgcgc ttgtcatccc gatggtgttg
      481 atcctgctca ccgttgtctt cttactcatg cgggtcgcac ccggtgatcc aatccaggcc
      541 gcgctcggtg gcaagctatc ggcggctcag ctcgaggaga agcggcgagc ggctggcttt
      601 accaagccgc tcatcgtgca atacgtcgaa tacctcaagc aggtcctcac gctgaatctg
      661 ggcacctcga tcaccgacaa ccaggccatc accacggtgt tgaaggtgaa cggtgcggcc
      721 actttggagc tgtcggttac ggccagcgtg atcgccttgg tcatcggcgt gccgatcggg
      781 ttgcgcgccg gccggctgcg cgacagcgct ttcgatgccg gtggccggct gttcggcatc
      841 atcacctacg ccgcgccggt cttcttcgtc ggcctattgt tccagctcgt cttcagcaag
      901 tggctgaact ggctgcccaa ctcggggcag ctaagcccga tcgaccaagc cgagttgccc
      961 gcgcacaccc acctgtccgt ggtcgatgcg atctgggacc acaacatgag tgcgtttgtc
     1021 gatgccgtga aacacctggc catgccgtcg ttcacgctcg ggctgttgct gtgcggcgtg
     1081 gtcatcaggt tgatccgcac caacctgatc cagaccctga agggcgacta cgtcgaggct
     1141 gcgcgtgcac gtggcgtgcc cgagcataag gtcgtcatgc ggcacgcgtt tcgcaatgcg
     1201 ctcgtgccgg tggtcacggt gatggggctg caagccgcat tgctgctgtc gggtgcggtt
     1261 ctcaccgaag agaccttcaa ctggccgggc atcggcaacc agctcgtcac gtatctcaac
     1321 aaccgtgact acattgccgt gcaaggcatc atcacgatct tcgcgctcgc cgtcgtcttc
     1381 atcagcttgc tcatcgactt cgtgaacgcc gccatcgacc cgagggtgcg gtactgatga
     1441 ctgctgccag tgtcgccgag cccggtggtg gctcgctgct cgatacgcca cccccgaccg
     1501 gcgagcggcc gcgcggaccg ttcggccgcc tgtgggaaca ggtcaagtcg gctcagggat
     1561 tctcgaagtg gttgttgtgg gtgggcgcgg cgatcacctt ggcgtttgtc gtactcgcgg
     1621 tattcgctcc actgctcgcg ccgtacggct tcgatcagta catgtcgcac ggcgtgcgct
     1681 tcgccaagca aggttcgccc agtggctcgc attggttcgg aaccaacgtt cgctcggtcg
     1741 acgtgttgtc ggtggtcatc tacgggtcgc gcaccgcgat cgaggtcgtc gtcctggccg
     1801 tggtgttcag cctggtgatc ggcgtgccgc taggcatgat ctccggtttc tacggtggct
     1861 ggctcgaccg gattctcgtg ctcatcatgg acgcactttt cgctttccct tacttgctac
     1921 tcgcgatcat cattgcgttc ctgttgcaga acagtgtcgg cagcggcgtg ctgacagccg
     1981 ccatcgcgat cacggtcatc tatgtaccgc agtatttccg tgtcgttcgg ggccaagtgc
     2041 tgtccgcccg agaagagcca tacgtcgagg ctgcacgcgc gttgggcgcg cgaccgcgca
     2101 cgatcatggg ccgctatctc ttcttcaacg tcatccagtc ggtgccggtc atcgcgacgt
     2161 tgaatgccgc cgacgcgatc ctcaccctcg ctgggttggg attcctcggc ttcggcatcc
     2221 agccgacgca ggccgccgaa tggggatacg aactgcagcg cggtgtcgcc gacatgggcg
     2281 ccggtctgtg gtggaccagc gtgtttcccg gcatcgccat cgtgtcgctg gtcacgggct
     2341 taacgctcgt cggcgagggc ctgaacgacg tgttgaatcc ggtgctgcgc aagagatcgg
     2401 gacgccgggt gctgctcccc aagcgagacg cggctgcatc ggccaccacg ttgccgagta
     2461 ggcaagtcga caaccacggt cgtggagagg agctgtcgtg acagcggctc ccgtgctctc
     2521 ggtgcgcgac ctccgtgtct ggtacggcac tgaccgagga ccggtgcgcg cggtcgatgg
     2581 tgtgtcgttc gacgtgcgcg caggtgagac gctcggtctc gtcggcgaat ccggctgcgg
     2641 aaagtccacc ctcggtcgcg gcatcctcgg cctgctccct agcggctcgg tcgtcgatgg
     2701 cagtgtgcgc ttccgcgatc gcgagctcgt cggcgtcaag caaagcgaca tgcgggagat
     2761 gcgcggagcc gagcttggac tcatcttcca agaaccgatg acccgcctcg acccgctcat
     2821 gcgcatcagt gagcacttcg ccgagacgtt gcgtgcacac gaacccgaac tcagctcaac
     2881 ggaaattgat gcgcgatcga tcgacacgct gcggcggatg ggtataccgc cgacgcgttt
     2941 ccggcactac ccacacgagt tctctggcgg catgcggcag cgcatcatga tcgcgcttgc
     3001 cctcgtgctc aacccggcgt ttgtcgtcgc ggacgagccg acgaccgcgc tcgacgtcct
     3061 ggtcgaggct cagatcctgt cgatcctgca cgacctgaag cgcaacttcg acacgtcgtt
     3121 gctgctgatc acgcacaacc ttgggattgt ggccgaggcc tgcgagcggg tggccgtcat
     3181 gtacgcggga cggattgtcg aggaaggcga cgcgcgccag gtgttctccg atccgtcgca
     3241 tccctacaca cgcgaactgc tgcgctcgac gatctcgttg tcgacgacgg cgttgcacta
     3301 cattccgggc tcgccgcccg atctggtcga gccgccgtcc ggatgtcatt tccacccgcg
     3361 atgcccgagc gcgatgcagg tctgccgcag tctcgatccg gtcgaggtgc tggcgcctag
     3421 cggtcagcga gtgtcgtgct ggttgcacgg tccggacgag cttctcccac cgggtggcgc
     3481 agccccactg caacgacagg agttggccag tgccgacgaa gcctgacaat gtcgatctcg
     3541 atctcgacgc gacgccactc gacgaccgac ccgtcgtgct cgacgtccgt gacttgcggg
     3601 tgcactttgc tttgcggggt gggttcttcg accggctact cggtcgctca tcgggatcgg
     3661 tgaaagcagt cgacggggtc tcgttcaccc tgcgcaaggg tgaggtgctc ggtttggtcg
     3721 gtgagtcggg cagcggcaag accacaacag cgcgtgcgct gcttggtctg gcaccgatca
     3781 ccggcggtac cgtgcgattc gacgcgggcc ccgattacgg cggcgcggtc gagctcacag
     3841 gtttgcggga gccacagttt cgtgcggtgc ggcaggcgat ccagctggtt tttcaggatc
     3901 cgcacgcgtc gttgaatccg gccatggatc tcgcggccgc cgttgctcat ccgttgaaga
     3961 ttcaccacat caccaaggac cgggcggaga tcgaccgccg ggttcgtgcc gcgttcgaac
     4021 gggtcgggct cgcgccggtc gaccagtacc tgcacaagta ccccgccg
//
LOCUS       DATCBJ010000455         2088 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_267120, whole genome shotgun sequence.
ACCESSION   DATCBJ010000455 DATCBJ010000000
VERSION     DATCBJ010000455.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2088)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2088)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2088
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_267120"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..246)
                     /locus_tag="VII50_09190"
     CDS             complement(<1..246)
                     /locus_tag="VII50_09190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5051064.1"
                     /translation="MRAPVLAALMVCLVAGCASKPLPTDSSAVSPSLTSLTAAAEPNK
                     QRADAEAAKLLALTPVPPGAAEIATAQADWSGPAMGTP"
     gene            complement(297..1433)
                     /locus_tag="VII50_09195"
     CDS             complement(297..1433)
                     /locus_tag="VII50_09195"
                     /inference="COORDINATES: protein motif:HMM:NF025382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4232 domain-containing protein"
                     /protein_id="HEY5051065.1"
                     /translation="MAARAVVELTRSHRVGAGDVGTIDVDDVSVVGLPVDAAVSAALQ
                     APVTAALAAYTKSVASESPCTGPACGSGDFQANFTTSRADSVVVSGTWTISTFYPGGA
                     HPTTQLTAVIVDGATGATITPSQLFAGSNLKSLAAVTATASKAKFDAIGCNPEFGNGV
                     APTADNYVGTAIGRTGLLIGLSQGQVAAEACGTVDLAIPWTAVQSQLSSIGARLAVTP
                     LPVAPAVIGAGAPTASPATAPRCATTSLDLALGVTKQTASTQYQVPIIFTNVSAQPCS
                     LLGFPGTDFGAGGTFPVSLVRTPAKPARVSLVPGGQAHAVLTYLAGPDPTCDAGGPWI
                     PSTLTITPPDDTTSVQIPWPGGSVDDCQTGATHPSSYIGPVVAS"
     gene            1405..1725
                     /locus_tag="VII50_09200"
     CDS             1405..1725
                     /locus_tag="VII50_09200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5051066.1"
                     /translation="MSSTTARAATTGAPLAAALDAAADAALEGAGDAGAVEANAAPLD
                     VRAVDVLAVRGDEGAADDATGVDVDVLACLEPELHAVAKVASATRAATRRYRRDDVGT
                     GLLK"
     gene            complement(1849..>2088)
                     /gene="glyA"
                     /locus_tag="VII50_09205"
     CDS             complement(1849..>2088)
                     /gene="glyA"
                     /locus_tag="VII50_09205"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720372.1"
                     /note="catalyzes the reaction of glycine with
                     5,10-methylenetetrahydrofolate to form L-serine and
                     tetrahydrofolate; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HEY5051067.1"
                     /translation="TVPYDTRKPFDPSGIRIGTPAITSRGMGVSEMRQIGAWFDEVVA
                     AVAKNDEDTVNATTDRVEAQVRELTADFPAPGLRV"
BASE COUNT          349 a          656 c          738 g          345 t
ORIGIN      
        1 cggagttccc attgccgggc cgctccagtc agcctgcgcg gtcgcgatct cggcggctcc
       61 aggcggaacc ggcgtcagcg ctagcagctt ggctgcttcc gcgtcggctc gctgtttgtt
      121 cggctccgca gcggcggtca acgaggtcag tgatggtgag acggctgacg agtcggtcgg
      181 cagcggtttc gacgcacacc ccgcgacaag gcagaccatc agcgcggcca ggactggggc
      241 tctcacaccc gtccgacgca ccccgcgccc ttccggtttc aggaacgcac cgcgactcag
      301 ctcgcgacca ccggaccgat gtagctgctg ggatgggtcg caccggtctg gcagtcgtcg
      361 acactgccgc ctggccacgg gatctgcacc gacgtcgtgt cgtccggcgg cgtgatcgtg
      421 agtgtcgaag gtatccacgg accgcccgca tcacacgttg gatccgggcc ggcgagatac
      481 gtcaggacgg cgtgggcctg accgccaggg acgaggctga ctcgagcggg cttggccggg
      541 gtgcggacca acgacaccgg aaatgtgccg ccagcaccga agtcggtgcc cggaaaaccg
      601 agcagcgagc acggttgcgc cgacacgttc gtgaagatga tcggaacttg gtattgcgtg
      661 ctggcggttt gcttagtgac tccgagcgcg aggtcgagcg aggtcgtcgc acagcgaggc
      721 gcagtcgccg gcgacgcagt gggtgcgccc gcaccgatga cggccggggc gaccggcagc
      781 ggcgtcacgg ccaagcgagc accgatgctg ctgagctgcg attgcacagc ggtccaggga
      841 atggcgagat cgaccgtgcc gcacgcctct gccgcgacct gtccctgcga gaggccaatc
      901 agaaggccgg ttcgcccgat ggcggtgccc acatagttgt cggcagttgg tgcgacgccg
      961 ttgccgaact caggattgca accgatcgcg tcgaacttcg ccttcgaagc ggtggctgtc
     1021 acggccgcca gtgacttgag gttcgaaccg gcaaatagct gggacggcgt gatggtggcg
     1081 ccggtcgcac cgtcgacgat caccgcagtg agttgcgtcg tcggatgcgc cccacctgga
     1141 taaaacgttg agatcgtcca ggtgcccgag acgacaacgg agtcggctcg gctcgtggtg
     1201 aagttggcct ggaaatcgcc gctcccacac gcggggcccg tgcacggcga ttccgacgcc
     1261 acggacttcg tgtaggcggc caatgcggcg gtgacgggag cctgtaacgc ggccgagaca
     1321 gccgcatcga cgggcagtcc gacgactgac acgtcgtcga cgtcgatcgt tccgacgtca
     1381 cccgcgccga cccgatgtga gcgggtgagt tcgacgacgg cgcgcgctgc cacaacgggt
     1441 gcgccgctag ctgcagcact ggatgccgca gcggacgcag cactagaggg tgctggcgac
     1501 gccggggcag ttgaagccaa cgccgcacca ctcgacgtcc gtgctgttga tgtgcttgcg
     1561 gttcgcggtg acgaaggcgc ggccgatgac gcgacaggtg tggacgtcga tgtgctggcg
     1621 tgcttggagc ccgagttgca cgcggtcgcc aaggttgcga gcgcgacgag agccgcaact
     1681 cgcaggtaca ggcgggatga cgtcggcacg ggtctcctca agtgacgttg gtcgaccatg
     1741 agacgtctac gaagcggact cggttgccag ggtgttgaag tccagcggtc gttcttgcga
     1801 ccgctggaca tcaatgcttg gccggcaggc tcggtgaagg gcagcgaact agacccgaag
     1861 gcccggtgct gggaagtcgg cggtcaactc gcgcacctgt gcttcgacgc ggtcggtcgt
     1921 cgcgttcacg gtgtcctcgt cgttctttgc taccgccgcg accacctcgt cgaaccaggc
     1981 cccgatctgg cgcatctcgc tgacgcccat cccgcggctg gtgattgccg gggtcccgat
     2041 gcgaatgcct gacgggtcga atggcttacg cgtgtcataa ggcacggt
//
LOCUS       DATCBJ010000456         2210 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_8998, whole genome shotgun sequence.
ACCESSION   DATCBJ010000456 DATCBJ010000000
VERSION     DATCBJ010000456.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2210)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2210)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2210
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8998"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            290..982
                     /locus_tag="VII50_09210"
     CDS             290..982
                     /locus_tag="VII50_09210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5051068.1"
                     /translation="MTVSDHLDIVAALREVPGVADADVEPDGEGDGLGLLRLGLAPGV
                     DEVEVASAVGRLLRERFGLGVDADRVQLVEDMQADAEDPAIEEDEHHDTDRFRPAIRR
                     MHIISSGLQVAAEVTLSISGSAARSAAETSAVGRAEGTATQSGVQRAVATATLRAVEE
                     LLGGRARFELDLVELTPTGRDRAVLVALTMLSTTGSEKLTGVAGVREDVRQAAIRATL
                     DALNRRVEHMVG"
     gene            1455..2045
                     /locus_tag="VII50_09215"
     CDS             1455..2045
                     /locus_tag="VII50_09215"
                     /inference="COORDINATES: protein motif:HMM:NF016144.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GrpB family protein"
                     /protein_id="HEY5051069.1"
                     /translation="MEVDPTLTERIRNSQEEQDAAWIYGPPTASGTVNVVEYDPNWPQ
                     LFSREQQRIHEILGDVALSVDHVGSTSVPGLAAKPIIDIDLVVPNSADESTYVPQLEA
                     AGYLLIIREPDWHEHRCLKGPDTNINLHVFSPGSPETLRHAVFRDWLRTHPDDRDLYA
                     RTKLDLASQDWSDTRGYTQAKDDLIDAIYLRAFGVA"
BASE COUNT          405 a          759 c          713 g          333 t
ORIGIN      
        1 agctcacgcc caaggccgcg ccgccgaaga aggccgtctc cgctaaatcg agcagtacga
       61 aggccgcgac caaatctgcc gctgcgaaaa cggccccgaa ggcgcccgct gcagccgcta
      121 ggcgggccgc tgcggagcgc aagcccgcgc gggcccgccg ttcggcctaa acgccaacct
      181 caacggcggc gcattcggcg cttaagcatc acggtcccag cgccgacttc aggcgtaaga
      241 ggaaggcgca cgggcgtctt cccacgggaa tttggatttg gagcccgccg tgaccgtcag
      301 cgaccacctc gacattgtcg cggcgttgcg tgaggtccca ggggtcgccg atgccgatgt
      361 cgaacccgat ggtgaaggcg acggactcgg gctgctccgg ctcggattgg ctccgggcgt
      421 cgacgaggtc gaggttgcca gcgcggtagg acggttgctc cgcgagcgat tcggcctggg
      481 cgttgacgcc gatcgtgtcc agctcgtcga agacatgcag gcggacgccg aagaccccgc
      541 catcgaggaa gacgagcacc acgacaccga ccgcttccgg ccggccatca ggcgcatgca
      601 catcatttcg tcgggactcc aggtcgccgc cgaagtcacc ctgtcgatct caggttcagc
      661 cgcacggtca gccgcagaga cctcggccgt cggccgcgcc gaggggaccg cgacccaaag
      721 cggggtccag cgcgcggttg cgaccgcgac tttgcgggcc gtcgaggagc tgctcggtgg
      781 ccgagcccga ttcgagctcg acctcgtcga gctcacaccg accggccgcg atcgcgccgt
      841 cctggttgcc ctcaccatgc tgagcacgac cggcagcgag aaactcacgg gtgtcgcagg
      901 agtccgcgaa gacgtccggc aggcggcgat ccgggccacc ctcgacgcac tgaaccggcg
      961 cgtcgaacac atggtcggct aaccgaccgg caccctgcac ccccaccttt ccagccgtcc
     1021 cggccattgg tttctcaacg gccgcaagaa cgccgcaaga acgcccgttc aggaccaatg
     1081 gtgcccaggc cgcggtaccc gtcgcggcgt ggcgtgacgg tcatgtcgat gatggggtcg
     1141 tcgtaagggc aggacccgcg accgtcgagc tacacgccgc gcgatcagat cgggcagcag
     1201 cctcggcgca tcaggatccg cgctccctcg ggtatcgtgc ggggcatgtc gatggggcgc
     1261 acgccggagc catcgcggtt catcaatcag ggcgaccaac tcgggccaca acctcctgcg
     1321 catccgcatc cgcatccgca tccgcgcaag ccgcgccggc accctctgcg caaactcata
     1381 gggtggctgg gtcgcaagcg gaacgcgtag cgccgtattc tggcgggctg ccttgaacct
     1441 gagaagagca ccgcatggaa gtcgacccga cccttaccga acgaatccgg aacagccaag
     1501 aggaacaaga cgcggcctgg atctacgggc cgccgaccgc cagcggaaca gtcaacgtcg
     1561 tcgagtacga cccgaactgg ccgcagcttt tcagtcgcga acagcaacgc attcacgaaa
     1621 tcctgggcga tgtggcgctg tcggtggacc acgtcgggtc gacgtccgta cccggtctgg
     1681 ccgcgaagcc gatcatcgac atcgacctcg tcgtgccgaa ctcagccgac gagtcgacat
     1741 acgttccgca actcgaagcg gccggctacc tgctgatcat ccgcgaaccc gactggcacg
     1801 aacaccggtg cctgaagggt cctgacacca acatcaacct gcacgtcttc tcgccgggca
     1861 gcccggaaac ccttcgccac gcggtgtttc gcgactggct gcggacgcac cccgacgatc
     1921 gggacctcta cgcccgcacc aagctcgacc tcgctagtca ggactggtcg gatacccgcg
     1981 gctacacgca ggcgaaggac gacctcatcg acgccattta tctgcgcgca ttcggcgtcg
     2041 cctgacggtc atctgcttag gatcaccgtc atggtgcgtc ctgaaggctg tctcgggcca
     2101 gcaggtgtga gttctgtctg ggtaggtatt ggagcgcccg gtagcaggcc ccggttcgtc
     2161 gtggagacgc ggcgggctaa gcggggtgtc aagtgcctgt ttggagggag
//
LOCUS       DATCBJ010000457         2461 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_535624, whole genome shotgun sequence.
ACCESSION   DATCBJ010000457 DATCBJ010000000
VERSION     DATCBJ010000457.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2461)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2461)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2461
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_535624"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..398)
                     /locus_tag="VII50_09220"
     CDS             complement(<1..398)
                     /locus_tag="VII50_09220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HEY5051070.1"
                     /translation="MSSNRDSSGRIVAQRPSQMPIHKYKPWTPVDLPDRTWPSQVITK
                     APRWLSTDLRDGNQALIDPMSPGRKLSMFQLLVRMGYKEIEVGFPSASQTDFDFVRSI
                     IENGEVPADVTISVLTQAREDLIERTVQSLV"
     gene            complement(493..1194)
                     /locus_tag="VII50_09225"
     CDS             complement(493..1194)
                     /locus_tag="VII50_09225"
                     /inference="COORDINATES: protein motif:HMM:TIGR01509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HEY5051071.1"
                     /translation="MSAAPALIFDFDGLLVDTESSALLAWREQFAVHDLVFPLEVWHR
                     FIGSQGSQEAMLAAVRAGGVDFEDAELRAEWRERHNRIVSSEPLRAGVEAFLDEAAAA
                     GAKFGVASSATRDWLDQQLGRLGIRDRFEIICARDNGRVKPAPDIYLAALASLGVEAS
                     DAIAFEDSPNGVAAAKAAGLYCVAVPNPVTADLPLGQADLRVESFTEISYPELVDRLR
                     GAAGYDPPNVEAPAP"
     gene            complement(1191..1622)
                     /locus_tag="VII50_09230"
     CDS             complement(1191..1622)
                     /locus_tag="VII50_09230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5051072.1"
                     /translation="MVQAAGYSGTPLATKLGIKDGGMLALVDAPKAWSVPDLPDGVTV
                     ARGTAESRRRGNGLAAVDVALVFCRAAVEVAAVPSLVGSLAAGSALWVAWPRKAGGHV
                     SDVDENLLREVLLPTGVVDVKVAALDVDWSALKFVWRKENR"
     gene            complement(1624..>2461)
                     /gene="era"
                     /locus_tag="VII50_09235"
     CDS             complement(1624..>2461)
                     /gene="era"
                     /locus_tag="VII50_09235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009080953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HEY5051073.1"
                     /translation="KSTLLNALVGQKVAITSDRPQTTRHAVRGIVHRPDAQLIVVDTP
                     GLHKPRTLLGERLNDVVRTTLAEVDVIVFCVPADEKVGPGDKFIAAELSRIEKTPVIA
                     VVTKTDAAERGEVASQLLALSRLGEWAEIVPVSAVAGEQVELLAELLIARLPEGPPLY
                     LDGELTDEPEMVMIAELIREAALDGVREELPHSLAVVVEEMKPREGKDLIDVFAVMYV
                     ERPSQKAIVIGTGGSRLREVGTRARLQAEALLGTRVYLDLRVKVAKDWQRDPAQLRKL
                     GF"
BASE COUNT          440 a          851 c          781 g          389 t
ORIGIN      
        1 acgaggcttt gcaccgtgcg ttcgatgagg tcttcacggg cctgcgtcag taccgaaatc
       61 gtgacgtcag ccggaacctc gccgttctcg atgatgctgc ggacgaaatc aaaatctgtc
      121 tgggacgccg aggggaaccc aacctcgatt tccttgtacc ccatccgcac cagaagctgg
      181 aacatcgaca gcttgcgccc cgggctcatc gggtcgatca gtgcctggtt gccgtcgcgc
      241 aggtcggtcg acaaccatcg gggtgccttc gtgatgacct gcgacggcca ggtgcggtcg
      301 ggcagatcga ccggcgtcca tggcttgtac ttgtggatcg gcatctggct cgggcgctgc
      361 gcgacgatgc gcccggaaga atcacggttg ctgctcattg ctgctcctcg gatggcgggt
      421 ggcggaatac ggccggcacg cagcaatccc gcgacgagga gaccggcctc ttagagggtc
      481 tcgccgcggc agctaaggag caggagcttc gacattggga ggatcatacc ccgccgcacc
      541 acgcaaacga tcgaccaatt ccgggtagga gatttcggtg aacgactcca cccgcaggtc
      601 ggcctgcccg agcggaagat ccgcggtcac cggattcggc acggcgacgc agtacaggcc
      661 ggccgctttg gcggcggcga ccccgttggg cgagtcctcg aacgcgatcg catcggacgc
      721 ctcgacaccc agcgacgcga gggccgccag gtagatgtcc ggcgctggct tgacgcgacc
      781 gttgtcccgg gcgcagatga tctcgaaacg atcgcggatg ccgagccggc cgagctgttg
      841 gtcgagccaa tcgcgggtcg cgctcgacgc caccccgaac ttggcccccg ccgcagccgc
      901 ttcgtcgaga aaagcctcga cgcccgcccg taacggctcc gagctgacga tccgattgtg
      961 gcgctctcgc cactccgcgc gcagttcagc gtcctcgaaa tcaacccctc cggcacgaac
     1021 ggcggcgagc atcgcctctt ggctgccctg ggagccgatg aaccggtgcc agacctcaag
     1081 cggaaagacc aggtcgtgga cggcgaactg ctcgcgccac gccagcagag cactcgattc
     1141 ggtgtcgacg agaagcccat cgaagtcgaa gatcagcgcc ggagccgcgc tcatcgattc
     1201 tccttgcgcc acacgaactt cagagccgac cagtcgacgt cgagcgcggc aaccttcacg
     1261 tcgacaacgc cggtcggcag caacacctca cgcaagaggt tctcgtcgac atcgctcacg
     1321 tgaccgccag ccttgcgcgg ccatgccacc cacagtgcgc tgcccgcagc gagggatccg
     1381 actagtgacg ggacggcggc cacttcgaca gcagcgcggc agaacaccag cgcgacgtcg
     1441 accgcagcca gaccgttgcc tcggcgacgc gactcggcag tgccgcgcgc gaccgtcacg
     1501 ccatcgggca ggtcaggaac cgaccacgcc tttggggcat cgaccaacgc cagcatcccg
     1561 ccgtccttga tgccgagctt cgttgccagc ggcgtgccgg agtaaccggc ggcctgcacc
     1621 acctcagaaa cccagcttgc gcagctgtgc gggatcgcgc tgccagtcct tcgcgacctt
     1681 cacccgcagg tcgaggtaga cgcgggtgcc cagcagcgcc tcggcctgca gtcgggctcg
     1741 cgtgccgacc tcgcgcaagc gcgagcctcc agtgccgatg acgatcgctt tctggctcgg
     1801 ccgctcgacg tacatgacgg cgaagacgtc gatcagatcc ttgccctcgc gcggtttcat
     1861 ctcctcgacg accaccgcca agctgtgcgg caactcttcg cggacgccgt caagcgcggc
     1921 ttcgcgaatc agctcagcga tcatcaccat ctcgggctcg tcggtgagct cgccgtcgag
     1981 atagagcggc ggcccctcgg gcagccgcgc gatcaacagt tccgcgagca actcgacctg
     2041 ctcaccggcg accgcggaga caggaacgat ctcggcccac tcgcccagac gcgacaatgc
     2101 gaggagctgg gaggcgacct caccgcgctc cgcagcgtcc gtcttggtca ccaccgcgat
     2161 gaccggcgtc ttttcgatgc ggctcaactc cgcggcgatg aacttgtcgc caggcccgac
     2221 cttctcgtcg gccggcacgc agaacacgat cacgtcgacc tcggcaagag tcgtgcgcac
     2281 gacatcgttg agtcgctcgc cgagaagggt gcgcggctta tgcaagccgg gcgtgtcgac
     2341 gacaatgagc tgcgcgtcgg gtcggtgcac gatgccacga acggcatggc gggtcgtctg
     2401 cggccggtcg ctggtgatcg ccaccttctg gcccacgagg gcattgagca gcgtcgattt
     2461 g
//
LOCUS       DATCBJ010000458         3073 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_12298, whole genome shotgun sequence.
ACCESSION   DATCBJ010000458 DATCBJ010000000
VERSION     DATCBJ010000458.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 3073)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3073)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3073
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_12298"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(236..1216)
                     /locus_tag="VII50_09240"
     CDS             complement(236..1216)
                     /locus_tag="VII50_09240"
                     /inference="COORDINATES: protein motif:HMM:NF020133.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5051074.1"
                     /translation="MIITRAPLRITLGGGGTDLPSYYEQFGGLVLSAAISKYIFISVN
                     RTFTPDYFLKYSALERVERVEQVEHPIIREVLLAHDIGPSVELVSMADIPSGTGLGSS
                     GAFTVGLLHAVYAFLRDHVTAGDLADEACAIEIDRLCRAVGRQDQYVAAFGGVKCFDF
                     KPDGQVAVSPLAMSTDTLHDLEEHLLMFFTGYSRSADMVLVEQKVRSQGGDTQMIDNL
                     HYVKELGLRSRAALEQGDTEGFAMLMHEHWEHKKKRSAAISNSSIDRWYQLGRDSGAL
                     GGKLVGAGAGGFLLFYTRDQRALRQTMAAEGLTEVRFTFDHDGSTVLARN"
     gene            complement(1337..2158)
                     /locus_tag="VII50_09245"
     CDS             complement(1337..2158)
                     /locus_tag="VII50_09245"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR02134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004801 - transaldolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HEY5051075.1"
                     /translation="MTSRRPSASMPPWRICPKPRPGFNSRSGHWRGLSGMTDLGLKTV
                     KVFADGANLEAMLELAGQPEIAGFTTNPTLMRQCGVSDYEGFARKLLEHITDRPISFE
                     VFADEHGEMLRQARLIASWASNVYVKIPVTDTRGTSTAAVVRELSDSGVHLNVTALMT
                     VPQVEVITSAVAGSPGAIISVFAGRVADTGRDPIPLMKAALETMAPYPELELLWASPR
                     EILNVWQADDIGVHIITVTHDLLKKLTLFGKDLDAFSLETVRMFHGDAATAGFFL"
     gene            complement(2046..2618)
                     /locus_tag="VII50_09250"
     CDS             complement(2046..2618)
                     /locus_tag="VII50_09250"
                     /inference="COORDINATES: protein motif:HMM:TIGR01656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEY5051076.1"
                     /translation="MIAKRSRPAVFLDRDGVLNEAFVRNGVPTPPRSLEEFRLLPGVV
                     EACADLRRAGFILIVVTNQPDIARGTQTRVEVDRMHQRLRSLVPLDEICVCPHDDSDA
                     CHCRKPQPGMLLDAAGRLDLDLLRSASIGDRWRDVEAARRAGVKAIYVECRYDEPTPF
                     GEYATVANLPEAAAWIQQQKWALEGALRDD"
     gene            complement(2615..>3073)
                     /locus_tag="VII50_09255"
     CDS             complement(2615..>3073)
                     /locus_tag="VII50_09255"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEY5051077.1"
                     /translation="FRFGNVVGPRQTHGVGFDFIRRLLDDPHCLRILGNGQQSKSYIY
                     VDDVLEAVLTAAQLATEAFSVFNVATGDYVTVTEIAELAVDVVGLPAGSTQFEYSGGD
                     RGWKGDVPVVRVNTDRIRALGWKNERTGPQALRASMESMLVDARAGRFDA"
BASE COUNT          567 a          979 c          953 g          574 t
ORIGIN      
        1 cggcgatggg cagcagagtc ttcgggatgg tcttggtctc aggccacatg cgggtaccga
       61 ggccgccggc caggatgacg cattgcatca atgcagccta gcgattgatg ccccgcttat
      121 gcccgacggc atactcggcc agctgacgct gccgtcgcag ctcgaactct tccgcgttcc
      181 aggcatctcc gctgcctgca tggacagcaa gtgtcgatct tgaaatggca agtggctagt
      241 tccgagcgag aacggtgctg ccgtcgtgat cgaaggtgaa acgcacttcg gtgaggccct
      301 cggcggccat cgtttggcgg agtgcgcgct ggtcacgagt gtaaaagagc aggaagccac
      361 ccgccccggc gccgaccagc ttgccgccaa gcgcaccact gtcgcggccg agctggtacc
      421 agcggtcaat gctgctgttg gaaatcgccg ccgaccgctt cttcttgtgc tcccagtgct
      481 catgcatgag catcgcgaag ccctctgtat cgccctgctc cagggccgct ctgctgcgca
      541 ggccgagctc cttcacgtag tgcaggttgt cgatcatctg ggtgtcgccg ccctgggagc
      601 gtaccttctg ctcgacgagg accatgtcgg cgctgcgaga gtagccagtg aagaacatca
      661 gcaggtgttc ctcgaggtca tgcagggtgt ccgtggacat ggccagggga gacacggcga
      721 cctgaccatc gggcttgaag tcgaagcatt tgacgccacc gaaagcagcc acgtactggt
      781 cctggcggcc gaccgctctg cacaggcgat cgatctcgat cgcacaggcc tcatcggcca
      841 gatcacctgc tgtcacgtgg tcgcggagga atgcatacac agcgtgcagc agccccacgg
      901 tgaatgcccc cgacgagccc agtccggtac cggacgggat atcggccatg ctcaccaatt
      961 ccactgacgg gccaatgtca tgggccagca aaacctcgcg gatgatcgga tgctcgacct
     1021 gctcgacgcg ctcgacgcgc tcgagcgccg agtacttcag gaagtagtcc ggcgtgaacg
     1081 tccggttgac cgagatgaag atgtacttcg agatggccgc ggacaacact aaaccgccga
     1141 actgctcata gtacgaaggc aaatcagtgc caccgccgcc aagcgtgatc ctgagcggcg
     1201 cacgggtgat gatcattcgt ccccctctcg aactcgtgct actgccggcg tctgcccggc
     1261 caatcgggaa gctgtcgcga ttgtgcatga atccgtgtcg aagatctccc tgcgcggatc
     1321 acgcgggctg cgatgctcac aggaaaaagc ccgcggtcgc ggcgtcgccg tggaacatgc
     1381 ggaccgtctc cagcgagaag gcgtcaaggt ccttgccgaa caacgtcagt ttttttagca
     1441 ggtcgtgcgt cacggtgatg atgtgcacgc cgatgtcgtc ggcctgccac acattcagga
     1501 tttcccgtgg gctggcccat agcagttcaa gctctggata gggcgccatg gtctcgagcg
     1561 ctgccttcat cagcggtatg ggatcccggc cagtgtccgc cacgcgaccg gcgaacaccg
     1621 agatgatcgc gccggggcta cccgccacgg cagaggtgat gacctcgacc tgcggcactg
     1681 tcatgagcgc ggtcacgttc aggtgcacgc ccgaatcgga caactctcgc acgacggcgg
     1741 ctgtgctcgt tccccgcgtg tcggtgacgg gtatcttgac gtagacgttg ctggcccacg
     1801 aagcgatcag ccgggcctgg cgcagcattt cgccgtgctc gtcggcgaac acctcgaatg
     1861 agatcggccg gtcggtgata tgttcgagca gcttgcgcgc aaagccttca tagtcgctga
     1921 ctccacattg acgcatcagg gtcgggtttg tcgtaaaccc tgcgatctct ggctgaccgg
     1981 ccagttcgag cattgcctcg aggttggccc cgtccgcgaa gaccttcacc gtcttgagtc
     2041 ctagatcagt catccctgag agccccctcc aatgcccact tctgctgttg aatccaggcc
     2101 gcggcttcgg gcagattcgc cacggtggca tactcgccga agggcgtcgg ctcgtcatag
     2161 cgacattcga cgtatatcgc tttgaccccg gcacgccggg cggcttctac gtcccgccat
     2221 cggtcgccga tgctcgcgct gcgcagtaga tcgagatcta gccgccctgc agcgtcaagc
     2281 agcatgcctg gctgaggctt gcgacagtgg caggcgtcag aatcgtcatg cggacacacg
     2341 cagatctcgt ccagcggcac caggctgcgc aaccgttggt gcatcctgtc gacctctacg
     2401 cgtgtttgcg tgccccgggc gatgtctggc tgattggtta cgacgataag aatgaagccg
     2461 gcccgccgca ggtcagcgca cgcctccacg acccctggca gcaggcggaa ctcttccagt
     2521 gatcgaggcg gcgttggcac gccgttccgg acgaatgcct cattgagtac tccgtcccga
     2581 tcgaggaaga cggccgggcg gctgcgtttc gcaatcaagc gtcgaaccgc ccggcgcgtg
     2641 cgtcgaccag catggattcc atcgatgccc gcaaagcctg gggcccggtg cgctcgttct
     2701 tccagcccag cgcacggatg cggtctgtat tcacgcgaac gacaggcaca tcgcccttcc
     2761 agccgcggtc ccctcccgag tactcgaact gggtgcttcc ggctggcagg ccgacgacgt
     2821 ccacggcgag ctcggcgatc tcggtgactg tgacgtaatc gccggtcgca acgttgaaca
     2881 ccgagaatgc ttccgtggcc agctgcgctg cggtcaggac ggcctcgagg acgtcgtcaa
     2941 cgtagatgta ggacttgctc tgctgcccgt taccaagtat gcgcaggcag tgcggatcgt
     3001 cgagaagacg gcggatgaaa tcgaaaccca cgccgtgcgt ctggcgaggg ccaacgacat
     3061 tgccgaaccg gaa
//
LOCUS       DATCBJ010000459         6151 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_82836, whole genome shotgun sequence.
ACCESSION   DATCBJ010000459 DATCBJ010000000
VERSION     DATCBJ010000459.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 6151)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6151)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6151
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_82836"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..253)
                     /locus_tag="VII50_09260"
     CDS             complement(<1..253)
                     /locus_tag="VII50_09260"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEY5051078.1"
                     /translation="MGIREIAQRRSGRLSTAMSAINARHPWNHNDHFHPWIVKRLANQ
                     RARGLDVGCGRGELLAVLAHHVREVHGIDVDHTMREAATA"
     gene            complement(1084..1833)
                     /locus_tag="VII50_09265"
     CDS             complement(1084..1833)
                     /locus_tag="VII50_09265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5051079.1"
                     /translation="MIGQLRSELFKQRTTRTAPILLASMAGVALLVVSLHVFALKSAD
                     LSQAANQPKVFGWGTTIGALFAALAGAIGITAEFRNGTIRPTLLANPDRTRVIAAKAA
                     AAAIAGLAIGLLAAGLVAAIGSAGLAARGITVRLGPGDFAQMIVGGGCGAALWAILGT
                     GIGALLRSQIAAVIGLCVWLLLIENILIGNLPSAAKYSPGASAGALAGLLPNAATATL
                     LAPAIGALLLIGYASVAAATGAIALQRRDID"
     gene            complement(1830..2597)
                     /locus_tag="VII50_09270"
     CDS             complement(1830..2597)
                     /locus_tag="VII50_09270"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY5051080.1"
                     /translation="MNQSTASAHDDRVVHGSEPDPPVVAVRELTKRFGAVVAVDALTF
                     SLDAGTVTGFLGPNGAGKTTTLRLLLGLARPTHGEALVFGHRYRDLSRPVRRVGALLE
                     SGDFDPGRSGRNHLRALALASDISFGRVEEMLELVELNAAASRAVGSYSLGMRQRLGL
                     AAALLGDPELLVLDEPANGLDAAGVHWLRGFLRQFAAAGGTVLVSSHLLAEVAQSVDH
                     AMIIDRGRLVATVDIAELADSGALEELYLELTGTDQS"
     gene            2596..3840
                     /locus_tag="VII50_09275"
     CDS             2596..3840
                     /locus_tag="VII50_09275"
                     /inference="COORDINATES: protein motif:HMM:NF019350.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HEY5051081.1"
                     /translation="MSATIAVRLAGRIVSRGCSLYPRWGMARPPCDATVAYAMHERHL
                     TILDAAVPAVVGAVIVVGETVHGGVSARPASIVLGSAAAVALVARRRWPGWTLVASGL
                     LVAVLFHLDRTAASAAVLAPAVALYSLALRRGRLQQVTAGLVAIAAVIAADVAHPGQP
                     TVEQTTVHVLLVALPLLAAEVIRTHRANMQLLVERFELAEQSREQDAERRAEQERMRI
                     ARELHDVVAHTLTEINVTAAAAAERAGVGDERSALEQIERSSHEAISELRGILGVLRG
                     PSPGTAPHAPPPRIDDIADMITRAHRAGLDVHLDVEGAPPARISDAMSLAAYRIVQES
                     LTNVRRYASGARATVTLRFGTSGVTVTIDNERGIGSGTINGGVGITGMRERVSAVGGQ
                     LSVGPHDAGFRVHAELPYEPTR"
     gene            3837..4502
                     /locus_tag="VII50_09280"
     CDS             3837..4502
                     /locus_tag="VII50_09280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009085178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEY5051082.1"
                     /translation="MIRVLVADDQAAVRDGFAALIDAQQTMNVVGRAANGREAVDLAR
                     RAFPDVILMDIRMPTLDGLEATRIICADPTLERTRVLVLTTFDLDEYVYGALRAGASG
                     FLLKDTPRAELLHAVEVIAAGEALLAPSITRRLIAEFAARRDPQQPSPRLAELTDRER
                     NILVLVACGLSNAEIAGQLVISPLIAKSHVRNILRKLDCHDRAGLVALAYENGLITPG
                     DQP"
     gene            4552..4731
                     /locus_tag="VII50_09285"
     CDS             4552..4731
                     /locus_tag="VII50_09285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5051083.1"
                     /translation="MAITTICRRCKEPIVAADEDDLVAQVQEHARDHGGAHGKHVPSR
                     EHILMHAQRDTPQGD"
     gene            complement(4858..5442)
                     /locus_tag="VII50_09290"
     CDS             complement(4858..5442)
                     /locus_tag="VII50_09290"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY5051084.1"
                     /translation="MIEVENLSKKYGDKLAVDDLSFTVQPGIVTGFLGPNGAGKSTTM
                     RMIAGLDRPTTGQVRVNGLDYRDACKMGANGSAQTTNRRTAQHGSVALTWENTTGDHR
                     TPSVITGRVLLLICGFGVQVPGGAPCLTWPFAVSRPPARATSDAVIGQMRAGSEQRSH
                     ASRQRSEICGCEARRRPSNMARLDGDVSRRRAPV"
     gene            complement(5495..>6151)
                     /locus_tag="VII50_09295"
     CDS             complement(5495..>6151)
                     /locus_tag="VII50_09295"
                     /inference="COORDINATES: protein motif:HMM:NF025687.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4386 domain-containing protein"
                     /protein_id="HEY5051085.1"
                     /translation="TLGMYSLVKGDRNFVLGSGSTNAVLWGCLLEVIVGLAGIGTAVT
                     LYPVVKRQNESAAIGFVASRTLEAAMIFTGVVSLVSLVSLRQTMGGSGAANAASLVAI
                     GASHAATYKATFLLGQTLMPGINALLLGSLMYRSRLVPRIIPTMGLIGAPLLITSTFA
                     TLLGHNSPTSAWSALATLPVAAWEFSLGVRLIVKGFNPSPITADDVPAVAAELQLVSA
                     "
BASE COUNT         1044 a         2057 c         2061 g          989 t
ORIGIN      
        1 gggcggtcgc ggcctcgcgc atcgtgtggt cgacgtcgat gccgtgaacc tctctgacgt
       61 ggtgagcgag tacggcgagc agttcgcccc ggccacaccc gacgtccaga cctcgcgccc
      121 gctgattggc aaggcgcttc acgatccagg gatgaaagtg gtcgttgtgg ttccacggat
      181 gccgggcgtt gatcgcgctc atcgccgtcg acaaccgccc gctgcgacgc tgcgcgatct
      241 cccggattcc cacgggcgca gcctctcatc ccgtcgacag tcctgcaccc aacgactcgt
      301 ttacggcgcc gacgctcgcg gatcgacgct catacctcgg tagaggcatg cgacgtcgcg
      361 cccgcggctt cacacttgga gccgctgcgg ccgcgcctgc tatccgcggc gccgaggcct
      421 gacgatcctc caccatggtc catgcacgaa gatgactgac gcggctgcgt ggagactact
      481 gatcggagca ccagtctcgg ccgcaacggc ctgcaccgcg cgaaccacga cggggtgcgc
      541 gaacaccgac ggcgaccggt cgggtcgaat cagcgaccgc cggatctcgg tagcgaactg
      601 gtcggcctca tcgaacccgg ccgcaaccac gccacgcagc gaggggaggt caaccaacga
      661 gtgccattgc ggaggatcgc agaagtgcat cagtcgcgtc gcgcccgtgc gaccaaaggc
      721 gttgctgtct ggtagtcgcg ttgggtccgg gtggcccaac ccggtaacgg cttcccacca
      781 gctcacccac tcgatggcag tccgggcccg cacgtcgtct tcgagttctc cttaccgatc
      841 cgggatgtcc cccggcgagc ggcggcggaa agcgttcatc cacgccttcg gtcagcccaa
      901 atgcgtccgc acccacaacg acaggtgcgg cagccgatcg acggacgcct caaaccgcca
      961 cgagtccgag ccagcggttc tcatcgtgca ctccgtcgtc tcgacgacgg gcaacgatgg
     1021 tgcccgccgc aagcgcagcc tatgtcgatt aggcggccgt cccttcgcgg tcacgggcgt
     1081 atgtcagtcg atgtcgcgtc gttggagcgc gatggcaccc gtcgcggccg cgaccgaggc
     1141 gtatccgatg agcagcagcg cgccgatggc gggggcgagc agtgtcgcgg ttgccgcgtt
     1201 gggtaggagt ccggcgagcg cgccggcgct ggcgcctggg gagtatttcg cggcggaagg
     1261 caggttgccg atcaggatgt tctcgatcag cagcagccat acgcaaagcc cgatgacggc
     1321 ggcgatttgg ctgcgaagga gcgcaccgat gcctgtgccg aggattgccc acagggcagc
     1381 gccgcagccg ccaccgacga tcatttgtgc gaagtcgccg ggaccgagcc gtacggtgat
     1441 gccccgcgcg gcgagtccgg cgctgccgat ggccgcgacg agtccggcgg caagcagtcc
     1501 aatggcgagt ccggcgatgg cagccgcggc tgctttggcg gcgatcacgc gggtgcggtc
     1561 ggggttggct agcagcgtgg gacggatcgt gccgttgcgg aactccgcgg tgatgccgat
     1621 cgcgccggcg agggctgcga acagtgcacc gatcgtggtg ccccagccga agactttggg
     1681 ctggttcgcc gcttggctta ggtccgcgga cttcagggcg aagacgtgca gcgagacgac
     1741 caacaatgcg acgccggcca tcgacgcgag caggatgggg gcagtgcgcg ttgtgcgttg
     1801 cttgaacagt tcggagcgca gttggccgat catgattggt ccgtgccggt gagttcgagg
     1861 tagagctcct cgagcgcgcc gctgtcagcg agctcggcga tatccacggt ggccaccagc
     1921 cgtccgcggt cgatgatcat cgcgtggtcc acgctttgcg caacctcagc aagcaggtgg
     1981 ctggacacga gcactgtgcc gccggcggcg gcgaactggc gcagaaaccc gcgcagccag
     2041 tgcacacccg ccgcgtcgag gccgttcgcg ggctcgtcga gcaccaatag ctccgggtcg
     2101 ccgagcagcg ccgccgccag tccgagccgc tggcgcatac cgagggagta gctaccgacg
     2161 gcgcggctcg ccgcggcatt cagctcgacg agttcgagca tttcctcgac gcgaccgaag
     2221 ctgatgtcgg aggcgagggc cagtgcgcgc aggtggttgc ggccgcttcg cccggggtcg
     2281 aagtcgcctg attccagcag cgcaccgacg cgccggaccg ggcgggaaag gtcacggtag
     2341 cggtggccga acacgagcgc ctccccgtgg gtggggcgtg cgagcccgag cagcaggcga
     2401 agggtggtgg tcttgcctgc accgttggga cccaggaagc cggtcacggt gcctgcgtcg
     2461 aggctgaacg tgagcgcgtc gacggccacg acggcgccga agcgtttggt cagctcgcga
     2521 accgccacca ccggcgggtc aggttcgctg ccgtgcacca cgcgatcgtc gtgagcggag
     2581 gccgtcgatt gattcatgtc ggcaacgatc gctgttcggc tggccgggcg catcgtctcg
     2641 agggggtgtt cgctttaccc ccgctggggt atggcgcgcc cgccgtgcga cgctaccgtc
     2701 gcctatgcca tgcatgaacg ccacctgacg atcctcgatg ccgcggttcc ggctgtggtc
     2761 ggcgccgtga tcgtggtcgg cgagacggtg cacggcggcg ttagcgcccg cccggcttcg
     2821 atcgtgctgg ggagcgcagc cgcggttgcg ctggtggcgc ggcgacgatg gcccggatgg
     2881 acgctcgtcg cctcgggact gctcgtcgct gtgctgtttc acctcgaccg cacggcggca
     2941 tcagcggcgg tgctcgcacc agcggtggcg ctatattcgc tcgcgctgcg ccgcggccgc
     3001 ctccaacaag tcaccgccgg gctcgtcgcc atcgcggccg tcatcgccgc cgacgtcgcc
     3061 catccgggac agccgacagt ggagcagacc acggtccacg tgctgctcgt cgcgctcccg
     3121 ttgctcgccg cagaagtgat ccgcacgcac cgggcgaaca tgcagttgct cgtcgagcga
     3181 ttcgagctgg ccgaacagag ccgcgagcag gacgcggaac ggcgcgccga gcaggaacgc
     3241 atgcgtatcg ctcgcgagtt gcacgacgtc gtcgcccaca ctctcaccga gatcaacgtc
     3301 accgcggcgg ctgcggccga acgcgccgga gtaggcgacg agcgcagcgc gctcgagcag
     3361 atcgaacgca gcagccatga agcgatcagc gagcttcgcg gcatcctcgg cgtgttacgc
     3421 ggtccgtcgc ccggcacggc tccacatgcg ccgccgccgc gcatcgacga catcgcggac
     3481 atgatcacca gggcacaccg cgcgggcctc gacgtgcacc tcgacgtcga aggcgccccg
     3541 ccagcacgga tctccgacgc aatgtcactt gcggcctacc gcatcgtcca agagtcgctc
     3601 acgaacgtcc gccgctatgc atcgggcgcc cgtgccaccg tcactctccg attcggcaca
     3661 tccggtgtga ctgtgaccat cgacaatgag cgaggcatcg gcagcggcac catcaacggc
     3721 ggtgtcggta tcactgggat gcgtgagcgc gtcagcgccg tcggcggcca actatcagtc
     3781 ggcccgcacg acgccggctt ccgcgtacac gccgaacttc cctacgagcc gacccgatga
     3841 tccgtgtgct ggtcgccgac gatcaggcag ccgtgcgcga cggcttcgcc gcactcatcg
     3901 atgcgcaaca aaccatgaac gtcgtcggtc gtgccgccaa cgggcgggag gcggtcgacc
     3961 tcgcccggcg tgctttcccc gacgtcatct tgatggacat ccggatgccc acgctggacg
     4021 ggcttgaggc cacccggatc atctgcgctg atcccaccct ggaacgcacc cgcgtgctcg
     4081 tcctgaccac cttcgaccta gatgaatacg tctacggagc attgcgcgcc ggcgcctcag
     4141 gcttcctact caaagacacc ccgcgcgccg agctgctcca cgccgtcgag gtcatcgcag
     4201 ccggggaggc cctgctcgcg cccagcatca cccgccgact catcgccgaa ttcgcggcgc
     4261 gtcgcgaccc gcagcagccc tcaccgcggc tcgccgaact caccgaccga gaacgcaaca
     4321 tcctcgtact cgtcgcctgc ggactcagca acgccgaaat cgcaggacaa ctcgtcatca
     4381 gcccactgat tgcgaaaagc cacgtccgca acatcttgcg caagctcgac tgtcacgatc
     4441 gtgccggact cgtcgcgctt gcatacgaga acggcctgat cactccaggc gatcaaccat
     4501 gatcccgcga aagccgcatc gagttatccg ctgcccacac tggagaacac catggccatc
     4561 accacgatct gtcgccgctg caaggaaccg atcgtcgcgg ccgacgagga cgatctcgtc
     4621 gctcaagttc aggaacacgc tcgcgaccac ggtggcgcac acggcaaaca cgtgccgagc
     4681 cgagaacaca tcctgatgca cgcgcagcgg gacacacctc aaggcgacta atagcgaccg
     4741 cgtgtggtgg cgaatgcggt ccatgtgggc ggtcctacga gattcggtgg gcctgctcgc
     4801 agatgaacga gcgcgcgctg gtcgggcatg caggaggtcg cagaagtctg ctggtgctca
     4861 aaccggcgcg cggcgccgtg agacgtcccc atcaagccgc gccatgttgg acggtcggcg
     4921 cctggcttcg cacccgcaga tttctgaccg ctgccgcgat gcgtgcgagc gctgctcgct
     4981 tcctgcacgc atttgcccga ttacggcgtc cgacgtagct cgggcaggtg gcctagaaac
     5041 tgcaaaaggc caggtcagac atggtgcgcc gccagggact tgaaccccga acccgcagat
     5101 taagaggaga acccgacccg tgatgaccga cggggtccga tgatcgccgg tggtgttttc
     5161 ccaggtcagc gccacactgc cgtgttgcgc tgtccgccgg ttggtggtct gtgctgaacc
     5221 gttcgcaccc atcttgcacg catcccggta gtccaagccg ttgactcgta cctggccggt
     5281 ggttggccgg tccaagccgg cgatcatgcg catcgtggtg gacttgcctg caccgttcgg
     5341 gccgaggaaa ccagtcacga ttcccggctg aacggtgaag ctcagatcgt cgacggcgag
     5401 cttgtcaccg tacttcttac tgaggttctc gacttcgatc atgggtgttc ctccgtgtat
     5461 gacgcagtgg gtcaatcgat agcgagcggg cttcttacgc cgagacgagt tgcagttcgg
     5521 cagcgacggc cggcacatcg tctgcggtga tgggcgacgg gttgaagcct ttgacgatca
     5581 gccgtacgcc gagtgagaac tcccatgcgg cgaccggcag tgtcgcgagc gctgaccacg
     5641 cggaggttgg cgagttgtgg ccgagcagcg ttgcgaatgt cgaggtgatg agcagcgggg
     5701 cgccgatgag tcccatcgtc gggatgatgc ggggcaccag cctggaccgg tacatcagcg
     5761 agccgaggag cagcgcattg attccgggca tcagggtctg cccgagcagg aaggtcgcct
     5821 tgtaggtcgc ggcgtgcgat gcgccgatgg cgaccagcga ggcggcgttt gcggctcccg
     5881 aaccacccat cgtctgtcgc agtgacacga gcgacacgag gctgacgacg ccggtgaaga
     5941 tcatggcggc ttcaagggtg cggcttgcga cgaagccgat cgcggcgctc tcgttttgtc
     6001 gtttgaccac cgggtacagg gtgaccgccg tgccgatacc ggcgagaccg acgatcacct
     6061 caagcaggca cccccagagc acggcgttgg tgctgccgga gccgaggacg aagttgcgat
     6121 cgcctttgac gaggctgtac atgccgaggg t
//
LOCUS       DATCBJ010000460         2591 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_349717, whole genome shotgun sequence.
ACCESSION   DATCBJ010000460 DATCBJ010000000
VERSION     DATCBJ010000460.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2591)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2591)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2591
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_349717"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            152..1417
                     /locus_tag="VII50_09300"
     CDS             152..1417
                     /locus_tag="VII50_09300"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014414.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane-fatty-acyl-phospholipid synthase
                     family protein"
                     /protein_id="HEY5051086.1"
                     /translation="MTTYELQPRQATDAPAGPWTQLPAVPRAPVHAAIARRIFIRAAA
                     RLGVTVELPDGRLLVKPAGKQTGRAAAPVMRLHRPAQFFRRLGHDGLIGFGESYMAGD
                     WDADDVASVLTPFARRMAGLVPGVLQRARRWYDARQPASEHNDLYGSRSNISRHYDLS
                     NELFALFLDQTMTYSSAIWEDESPENLATAQVRKIDRLLDLAGVRAGTRLLEIGTGWG
                     ELAVRAACRGAKVTTVTLSREQQAWAIRRANLAGVGDLVDVELRDYREVTGTYDAVVS
                     VEMIEAVGLEYWPAYFAAIDRVLAPGGRVALQSITIDHQRLLATKGSYTWIHKYVFPG
                     GIIPSLHAVRDVVAHHTLLRVSSATAYGHDYARTLHSWLERFHANTAAVHALGFDERF
                     TRMWRFYLAYCEAGFASGYLNVHQLVLER"
     gene            complement(1326..2081)
                     /locus_tag="VII50_09305"
     CDS             complement(1326..2081)
                     /locus_tag="VII50_09305"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEY5051087.1"
                     /translation="MTASVENVDAARARPLQPVLVRQPGATAVRAVALVLHGGREVSV
                     APVTAHQLAVLRMVPIARHLASAGEADGLGVWRLRFRYRGWNSDAHPVADVRWAVQQL
                     REQHGDVPIVLVGHSMGGRAALWAADEPGVVGLVGLAPWVTPNEPYKQVVGRRLLVVH
                     GTRDRTTSAKNSKALVDAVRDASSPDEPSADATFVHLRGGGHAMLRRAGTWNDLTADF
                     VLGVSVPARVDARSGNRSRSRPRSRRGRTSTSG"
     gene            2181..>2591
                     /locus_tag="VII50_09310"
     CDS             2181..>2591
                     /locus_tag="VII50_09310"
                     /inference="COORDINATES: protein motif:HMM:NF015909.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5051088.1"
                     /translation="MENSASTPVSHADLRKRIAPFLVKPRAVVRLGDDSKDFDPGYRD
                     QFVTRADAGVALAEGVRQLGEYQARLAAQSTYAVLVILQAMDAAGKDGTIKHVLSGVN
                     PQGVSVHPFKVPSLIELEHDYLWRYQLQLPQRGSI"
BASE COUNT          414 a          893 c          856 g          428 t
ORIGIN      
        1 cgaaggcgac gacactacga acactggcgc actacccgtt gggggcgttg cgcgtgaccg
       61 tgctgattcg ctaccaaggc atcaagctct tcttgcgcgg cctgccggtc gttcgtcggc
      121 cgaccccgag tgatcccacg aaaggtcagg cgtgactacc tacgagctgc aaccacgaca
      181 ggcgaccgac gcaccagccg gtccgtggac ccaactgccc gccgtcccgc gtgctcctgt
      241 ccatgccgcg atcgcccggc ggatcttcat cagagcagcg gcgcgcctcg gcgtgaccgt
      301 cgagctgccc gacgggcgcc tgctggtcaa acccgctggg aagcagaccg gtcgggccgc
      361 ggcacctgtc atgcggctgc accgtcctgc ccagttcttc cgtcgtcttg gccacgatgg
      421 cctgatcggc tttggggaga gctacatggc aggcgattgg gacgccgacg acgtggcctc
      481 cgtcctcacg ccgttcgcgc gaaggatggc cgggctcgtc cctggcgtgc tgcagcgcgc
      541 gcgtcgctgg tacgacgccc gccaaccggc gtccgagcac aatgacctgt acggatcgag
      601 gtcgaacatc tcccggcact acgacttgtc caacgagctg ttcgcgctct ttctcgacca
      661 gacgatgacc tactcgagcg cgatctggga ggacgaatcc cccgagaacc tggccaccgc
      721 gcaggtgcgc aagatcgatc ggctgctgga tctcgccggc gtgcgggcgg gcactcggtt
      781 gctcgagatc ggtactggct ggggtgagct cgcggtccgt gccgcgtgtc gaggcgccaa
      841 ggtcacgacg gtgacgctct cacgagagca acaggcgtgg gcgatccggc gggctaactt
      901 ggccggcgta ggcgacctcg tcgacgtcga gctccgcgac taccgcgagg tcaccgggac
      961 ctacgacgcc gtcgtgagtg tcgagatgat cgaggcggtc ggtctggagt actggccggc
     1021 ctacttcgcc gcgatcgatc gcgttcttgc acccggcggc cgggtcgcgc tgcagtcgat
     1081 caccatcgat catcaacggc tgctcgcgac gaagggctca tacacctgga tccacaagta
     1141 cgtgttcccc ggtggcatca tcccgtcgct gcacgccgtc cgcgatgtcg ttgcacacca
     1201 cacgttgcta cgggtgagct ccgcgaccgc ctacggccac gactacgcgc gcacgctgca
     1261 ttcgtggctg gagcgattcc acgcgaacac cgcggcggtc catgcgctcg ggttcgatga
     1321 gcggttcacc cggatgtgga ggttctacct cgcctactgc gaggccggct tcgcgagcgg
     1381 ttacctgaac gtgcatcaac tcgtgctgga acgctaactc ccaggacgaa atccgcggtc
     1441 aggtcattcc acgtcccggc acggcgcagc atcgcgtggc caccgccgcg cagatgcacg
     1501 aacgtcgcgt ccgcggaagg ctcgtccggc gatgatgcgt cgcgaacggc gtccacgagt
     1561 gccttggagt tcttcgccga cgtcgtgcga tcacgggtgc catgcacgac cagcaggcgg
     1621 cgtccgacca cttgcttgta cggctcgttc ggcgtcaccc acggtgcgag gccgacgagg
     1681 ccgacgaccc cgggctcgtc ggcggcccac agcgccgcgc ggccacccat tgagtgaccg
     1741 acgagcacga tcggcacgtc gccgtgttgc tcgcgtagct gctgcacagc ccagcggacg
     1801 tcggcgaccg gatgcgcgtc gctgttccag ccgcggtacc ggaagcgcag ccgccatacg
     1861 ccgagtccat ccgcctcgcc agccgacgcg aggtgacgcg caatcggcac catgcgcaag
     1921 accgcgagct gatgcgccgt gacgggtgcg acgctgacct ctcggccccc atgcagcacc
     1981 agcgccaccg cgcgcaccgc cgtggcgcct ggctgccgca caaggacggg ttgaagcggc
     2041 ctcgcgcggg cggcatcgac gttctccacg ctcgcggtca agcgcgtggt cctctcgttg
     2101 gtggcggtat tcgccgcgtc cagtgtgtcg cgggttcgtt ggccgtgacg ttcccggttc
     2161 agacccggca tgatgcgcag atggagaact ccgcgtccac cccggtcagt cacgccgatc
     2221 tacgcaagcg gatcgcgccg tttctggtca aacctcgtgc ggttgtgcgg ctgggcgacg
     2281 attccaagga cttcgacccg ggctaccggg accagttcgt caccagggcg gacgccgggg
     2341 tggcactggc cgagggtgtg cggcagctcg gcgagtacca ggcgcggctg gcggcgcaga
     2401 gcacctacgc ggtgttggta atcctgcagg cgatggatgc cgccggcaag gacggcacca
     2461 tcaaacacgt cctcagtggc gtcaatccgc agggagtgag cgtccacccg ttcaaggtgc
     2521 catccctgat cgagctcgaa cacgactacc tgtggcgcta ccagttgcaa ctgccccagc
     2581 ggggctcgat c
//
LOCUS       DATCBJ010000461         7040 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_110310, whole genome shotgun sequence.
ACCESSION   DATCBJ010000461 DATCBJ010000000
VERSION     DATCBJ010000461.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 7040)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7040)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7040
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_110310"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..553
                     /locus_tag="VII50_09315"
     CDS             <1..553
                     /locus_tag="VII50_09315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HrcA family transcriptional regulator"
                     /protein_id="HEY5051089.1"
                     /translation="VEQRQVDLPGLIDERTVSDLRARLNQRIAGRPIHDVPNDLADLA
                     EAEPPATRGNASAVVAVLLASLVERREERVVVGGASNLTRLALDFPDSVHPVLEALEE
                     QVVLIRLLGEASRPTEVTVRIGDENSHEGLRSTSIVSATYGSGSRALASLGVLGPMRM
                     DYPGTMAAVGAVARYVGRILAES"
     gene            591..1715
                     /gene="dnaJ"
                     /locus_tag="VII50_09320"
     CDS             591..1715
                     /gene="dnaJ"
                     /locus_tag="VII50_09320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HEY5051090.1"
                     /translation="MARDHYAILGVRKDAPPEEIKRAYRRLARQLHPDVNPDAETQER
                     FKEITAAYEVLSDPSKRQLYDMGGDPLSASGNNPFNAGFGGLGDIMDAFFGGGASRGP
                     RTRTRKGNDALIHVEIDLVEAVFGATRELNIETAIVCATCDGAGTASGTHPETCQVCK
                     GRGEVSQVTRSFLGQVMTSRPCPQCGGVGTVIRHPCPECSGDGRVRARRTVEVGIPAG
                     VEHGMRLRLTGEGEVGPGGGPPGDLYVEIEERKHPTFERHGVDLHAAVPVPMTAAALG
                     TSLKVETLDGPVDIDVKPGTQSGTVIPLRGRGVPRLRSNSRGDLHVHLEVTTPTKLDA
                     EQEELMRKLASLRGEESPDGAFKAATPGIFGRIRDAFNGR"
     gene            1722..2480
                     /locus_tag="VII50_09325"
     CDS             1722..2480
                     /locus_tag="VII50_09325"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: protein motif:HMM:NF008693.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HEY5051091.1"
                     /translation="MTAPLFFADVDRLAAAEIGDVVELGGAEGRHAATVRRLVAGERL
                     DLSDGAGLLCVAEVTAVQGQVLHARILERSRVPRPAPRITVVQALAKGDRDERAVETM
                     TEVGVDVIVPWQASRSIVRWDSPRAQKAVTRWRSTAREAAKQARRRWLVDVPLLEATS
                     AVVARLGSAVGAGGVAVVLHEAADAPLRGLVVPPDGEVVLVVGPEGGIADDELAAFEA
                     AGAVIRRLGPTVLRTSTAGTVAAAIVMANSGRWG"
     gene            complement(2490..3671)
                     /locus_tag="VII50_09330"
     CDS             complement(2490..3671)
                     /locus_tag="VII50_09330"
                     /inference="COORDINATES: protein motif:HMM:NF022112.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gmad2 immunoglobulin-like domain-containing
                     protein"
                     /protein_id="HEY5051092.1"
                     /translation="MSDRPGPFDPADDASADDQTARRLREVLAREARAITPSADGLSM
                     IREKIRAQRPTRRNWVRAFQIGGAGLATAAVAIIGVAVVQHTSGSTAHPAATAAAASS
                     ASPSAPAPVVPPATPAASAAVASSAPSSYPVWIYYVDKRKDPQQLLFRESTTWPSSPQ
                     HTFVKDAVSAMLATPAQDPDYTSYWPAGTTLLSANIVGGTTAVVNLSSEASTGPANEA
                     AISAQQLLYTIIAAAPKIDSLQLQIAGVPVSSLWGTPLAATATATASATASATAGELA
                     GAAASGSAAATVAALPVWQVWGHVWITSPAQDATVASSFQITGEATVFEGTVHWQILR
                     DGGLLKQGSVTATEGAPGRGTWSVTVSGLVPGPYTVKAYEVSAKDGSITYEDDKAFTV
                     S"
     gene            complement(3668..4180)
                     /locus_tag="VII50_09335"
     CDS             complement(3668..4180)
                     /locus_tag="VII50_09335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SigE family RNA polymerase sigma factor"
                     /protein_id="HEY5051093.1"
                     /translation="MLGQFETATADEAVVELYRAHYAGLVRLSAMLVGDVGTAEELVQ
                     DAFVAMHGSWGRLRDPEKALAYLRQAIVNRSRSALRHRAVVAKYPQAPPPDVPSAEHF
                     AVAATERDQLMRAIHALPRRQRETLLLRYYSDLSEAQIADTLGISQGAVKSHASRGLA
                     ALRTLLETTP"
     gene            4485..5447
                     /locus_tag="VII50_09340"
     CDS             4485..5447
                     /locus_tag="VII50_09340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HEY5051094.1"
                     /translation="MVSLLGSRDELLRIIERAFDSDIHVRGNEITITGAGPENAVVAQ
                     LFEELIALLGSGTTLTPDAVERSVTMLRSKEPERPVEVLTLDIVSSRGKTIRPKSVNQ
                     KRYVEAIDRHTIVFSIGPAGTGKTYLAVAKAVQALQAKQVNRIILTRPAVEAGERLGF
                     LPGTLYEKIDPYLRPLYDALHDMLEPDSIPRLMQSGTIEVAPLAYMRGRTLNDAFIIL
                     DEAQNTTPEQMKMFLTRLGFGSKMVVTGDVTQVDLPGNTQSGLRVVRDILDGIPDVHF
                     SMTGSGDVVRHKLVSEIVDAYERFDATTDAAPPRPVSARRRGGR"
     gene            5448..5960
                     /gene="ybeY"
                     /locus_tag="VII50_09345"
     CDS             5448..5960
                     /gene="ybeY"
                     /locus_tag="VII50_09345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HEY5051095.1"
                     /translation="MSVEVANESGIAVDELRLVAVARHVLDAMRIHPLAELSILLVDE
                     GEMTVLHERWMDEPGPTDVLSFPMDELRPGRDDRDVDETDDAGMLGDVVLCPAVAAKQ
                     GHVAGHSVQDELELLCTHGILHLLGFDHAEPDDEKAMFGLQRDLLTAWQSARTAQPAQ
                     STPTAESTGA"
     gene            5957..>7040
                     /locus_tag="VII50_09350"
     CDS             5957..>7040
                     /locus_tag="VII50_09350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HEY5051096.1"
                     /translation="MNPGDVLLPVSVVVLIFVAGACAAADAALSRVSRVHVDDYLRDN
                     RRGAVALRRVIAEPPRYLNVVLLLRVAAEMTAAVMVAVWAIGRFGKSWDTVLATAAAM
                     VVVSYVVVGVAPRTLGRQHADGVALFSAPWVVRLAGILGPLPKFLIALGNALTPGQGF
                     RDGPFTSEAELRDMVDLAEASKVIESDERQMIHSVFELGNTIVREVMVPRTDIVFIEQ
                     TKTLRQAMSLALRSGFSRLPVVGENEDDVVGTVYLRDITRRVFEYRESESAERVESLM
                     RPATFVPDSKPADVLLREMQAERIHLVIVVDEYGGTAGLATIEDILEEIVGEITDEHD
                     REGPRIERLPDGGVRVTARLPVDELAE"
BASE COUNT         1120 a         2364 c         2419 g         1137 t
ORIGIN      
        1 ggtcgagcag cggcaggtcg acctgcctgg cttgatcgac gagcggactg tcagcgacct
       61 gcgtgcccgc ctgaaccagc gcatcgccgg tcggccgatc cacgacgtcc cgaatgatct
      121 ggccgatctc gccgaagccg aaccgccggc cacacgtggc aacgcctcgg ccgtcgttgc
      181 cgtactcctc gcgtcacttg tcgagcggag ggaggagcgc gtcgtcgtcg gcggcgcgtc
      241 gaacctcaca cggttggcgc tcgacttccc agactcggtt catccggtgc tcgaagcact
      301 cgaagagcag gtcgtgctca ttcggctgtt gggcgaagca agcaggccca ccgaggtcac
      361 cgtgcggatc ggcgacgaga actcgcacga gggtctgcga tcgacctcga tcgtgagtgc
      421 gacgtacggc tctggcagtc gagctctcgc cagtcttggg gtgctcggtc cgatgcggat
      481 ggactatccg ggcaccatgg cggctgtcgg cgcggtcgca cgttatgtcg gacggattct
      541 cgccgagtcc tgaagtaccg aagtactaac gacgcaacag ggagtgaccg gtggcgcgcg
      601 accattacgc gatcctcggc gtgcgcaagg acgcaccgcc cgaggagatc aagcgcgcgt
      661 accggcggtt ggctagacag ctgcaccctg acgtcaatcc cgatgcggag acgcaggagc
      721 gcttcaagga gatcaccgcg gcctacgagg tgttgtccga cccgagcaag aggcagcttt
      781 acgacatggg cggcgatccg ctgtcggcgt ccggcaataa ccctttcaac gctggcttcg
      841 gcggcctcgg cgacatcatg gacgcgttct tcggcggtgg tgcgagccgc ggaccgcgga
      901 cccgtactcg caagggcaac gacgcgctga tccatgtcga gatcgatctg gtcgaagcgg
      961 tgttcggcgc cacccgcgag ctcaacatcg agacggccat cgtgtgcgcg acctgcgacg
     1021 gcgcgggcac tgcatcgggc acgcatccgg agacctgtca ggtgtgcaag ggtcgcggcg
     1081 aggtcagcca ggtcacccgg tcgttcctcg gccaggtcat gacgtcgcga ccatgcccac
     1141 agtgcggcgg cgttggcacg gtgatccggc acccatgccc ggagtgctcg ggtgacggcc
     1201 gggtgcgcgc gcggcgcact gtcgaggtgg ggatccccgc gggcgtcgaa cacggcatga
     1261 ggttgcggtt gaccggcgaa ggtgaggtcg gtcccggcgg cggtccgccc ggcgatctct
     1321 acgtcgagat cgaagaacgc aagcatccga ccttcgagcg ccacggtgtc gacttgcatg
     1381 cagctgtgcc ggtgccgatg accgctgccg cgctcggcac atccctaaag gtcgagaccc
     1441 tcgacggccc ggtcgacatc gacgtgaaac ccggcaccca gtccggcacc gtgatcccgt
     1501 tgcgcggccg cggggtgccc cggctgcgct cgaacagccg cggcgatctg catgtgcacc
     1561 tcgaggtcac gacgccgacg aaactcgatg cggagcagga agagttgatg cgcaagcttg
     1621 cgagcctgcg cggcgaagag tcaccggatg gcgcgtttaa ggctgcgacg cccgggattt
     1681 tcggccgcat ccgcgacgcg ttcaacggcc gctgagtcgt cttgaccgca ccgctcttct
     1741 tcgccgacgt cgaccggctc gccgcagcgg aaatcggcga cgttgttgaa ctgggcggcg
     1801 ccgaaggccg gcacgccgca acggtccgtc ggctggtggc gggcgagcga ctcgacctct
     1861 cggacggcgc cggactgctg tgcgtggcgg aggtgaccgc cgtccagggt caggttttgc
     1921 acgcccgcat tctcgaacgc tcgcgggtgc cacgacctgc accccggatc accgtcgtgc
     1981 aggcattggc caagggcgac cgcgacgagc gggccgtcga gaccatgacc gaggtaggcg
     2041 tcgacgtcat cgtgccgtgg caggcgtcgc gatcgatcgt gcgctgggac tcgccgcgcg
     2101 cgcagaaggc cgtcacccgc tggcgctcga ccgcccgtga agcggcgaag caggcgcgac
     2161 gacggtggct ggtcgacgtc ccgctgctcg aagcaacatc cgcggtggtg gctcggctcg
     2221 ggtctgcggt tggggccggg ggagttgctg tcgtcctgca cgaggcggcg gatgcgccgc
     2281 tacgcgggct ggtggtcccg cccgacgggg aggtcgtgct ggtcgtcggt ccggagggcg
     2341 gcattgccga cgacgagctg gccgcgttcg aagccgctgg cgccgtcatc cgccgcctcg
     2401 gacctaccgt cttgcgcacc tcgaccgcag gcaccgtcgc ggcggccatc gtgatggcca
     2461 actccggccg ctggggctag tcgcgaccgt cagctgacgg tgaaggcctt gtcgtcttcg
     2521 taggtgatcg atccgtcctt cgccgacacc tcgtacgcct tgaccgtgta ggggccgggg
     2581 acgagcccgg agaccgtcac gctccaggtt ccacggcccg gcgcgccctc ggtcgcggtc
     2641 acggagccct gcttcaagag accgccatcc cgaaggatct gccagtggac ggtgccttcg
     2701 aacactgtcg cctcgccggt gatctggaag gacgacgcga cggttgcgtc ttgggcgggc
     2761 gacgtgatcc atacgtggcc ccatacctgc cacaccggca gcgcggccac cgtcgccgcc
     2821 gcgctcccgc tcgccgccgc gcccgccagc tcgcccgccg tcgcgctcgc tgtcgcgctc
     2881 gctgtcgcgg tcgcggtcgc ggccagcggg gttccccaca gcgagctcac cggcacgccg
     2941 gcgatctgca gttggaggct gtcgatcttc ggtgcggcgg cgataattgt gtacagcagt
     3001 tgctgcgcac taatggccgc ctcattcgcg ggtccggtgc tcgcctcgct cgacagattc
     3061 acgacagccg tcgtgccgcc aacgatgttg gccgacagca acgtggtgcc ggccggccag
     3121 tagctggtgt agtccggatc ctgcgcaggt gtcgcgagca tcgcgctgac cgcgtccttg
     3181 acgaacgtgt gctgcgggga cgacggccag gttgtgcttt cgcggaacag cagctgctga
     3241 gggtctttgc gcttgtcgac gtagtagatc cataccgggt agctgctggg cgccgacgaa
     3301 gccaccgcgg ccgaggcggc tggagtcgcg ggaggcacga cgggtgccgg cgccgaaggt
     3361 gacgccgatg acgcggccgc cgcagtggcc gcagggtgag cggtggagcc ggacgtgtgc
     3421 tgcaccacag ccaccccgat gatcgcgacc gccgccgtcg ccaggccggc gccgccgatc
     3481 tggaacgcgc gtacccagtt acgccgcgtc ggccgctgag cgcggatctt ctcgcggatc
     3541 atcgacaggc catcggcgga cggcgtgatg gctcgcgctt cgcgcgccag cacttcgcgc
     3601 agccggcggg cagtttggtc gtcggcggat gcgtcgtccg cggggtcgaa cggacccggg
     3661 cggtcgctca tggcgtcgtc tccaagaggg tgcgaagggc ggccaggcca cgggatgcgt
     3721 ggctcttgac ggcgccctga ctgatgccga gggtgtcggc tatttgggcc tccgagagat
     3781 cggagtagta ccgcagcagg agggtttccc gctgccggcg aggtagtgcg tggatcgccc
     3841 gcatgagctg gtcacgttcg gttgcggcta ctgcgaaatg ctctgcactc gggacgtcgg
     3901 gtggtggtgc ctgcggatat ttcgccacca ctgcgcggtg ccgcaaagcc gaccgggagc
     3961 ggttgacgat cgcctgccgg aggtaggcca gtgccttctc cgggtcgcgc agccggcccc
     4021 agctgccgtg catggcgacg aaggcgtcct ggacgagttc ctctgcggtt ccgacgtcgc
     4081 cgaccaacat ggcggaaagg cgcactagcc ccgcgtagtg ggctcgatat agctcgacga
     4141 ccgcttcgtc tgcggtggcc gtctcgaatt ggcccagcaa ggttcggctc cccgggcggc
     4201 tcaagagtgc ggcggtcata cggatacgac gcgcatccgc atgggtcggt ttaccggagt
     4261 tgtttaccgc atggcgcccc gatggcgcgc gggtacgatg ggagggtcac gtcaccgagg
     4321 accgaaagca ggcgcgagca ggccccaccg gccgatccat gcgagccgaa ccgagcccgg
     4381 cccatcccgc ggcgcgtccc acgcgcaggc ccgcacgtga cggagccggc tccaccccga
     4441 cgcagacggc gcagacccgc attgtcgtgc caggtgcaca ctcgatggtc agcctgctcg
     4501 gatcccgcga cgagctgctg cgcatcatcg aacgtgcttt cgacagcgac atccacgtgc
     4561 gcggcaacga gatcaccatc accggcgccg gcccagagaa cgccgtggtc gcccagctgt
     4621 tcgaagagct gatcgcgctg ctcggcagcg ggaccacgct gactcctgac gccgtcgaac
     4681 gcagcgtcac catgttgcgc agcaaggagc cagaacgacc ggtcgaggtg ctcaccctcg
     4741 acatcgtgtc ctcgcgcggc aagacgatcc ggcccaagtc ggtcaaccag aagcgctacg
     4801 tcgaagcgat cgaccgccac accatcgtgt tctcgatagg cccggccggt accggcaaga
     4861 catacctggc cgtggccaag gccgtgcagg cgctccaagc caagcaggtc aaccgcatca
     4921 tcttgacccg cccggccgtc gaggcgggcg agcggctcgg ctttctgcca ggcacgctct
     4981 acgagaagat cgacccctac ctgcggccgc tctacgacgc cctgcacgac atgctcgaac
     5041 ccgactcgat cccgcggctg atgcagagcg gcacgatcga agttgcgccg ctggcctaca
     5101 tgcggggccg cacgctcaac gacgccttca tcattctcga cgaggcgcag aacacgactc
     5161 ctgagcaaat gaagatgttc ctgactcggc tcggattcgg ctcgaagatg gtcgtgacgg
     5221 gtgacgtcac ccaggttgac ctgccaggca atacccaaag tggccttcgc gtggtgcgcg
     5281 acatcctcga cgggattccc gacgtgcact tctcgatgac cggcagcggc gacgtggtcc
     5341 ggcacaagtt ggtcagcgag atcgtcgatg cgtatgaacg gttcgacgcg acgaccgatg
     5401 ccgctcctcc gcggccagtg tcggctcgcc gccgcggcgg ccgctgagtg agcgtcgagg
     5461 tcgccaacga atcaggcata gctgtcgacg agcttcgcct tgttgcggtc gcccgacacg
     5521 tcctcgacgc catgcgcatc cacccacttg ccgaactgtc catcctgctc gtcgacgagg
     5581 gcgagatgac cgtgctccac gagcgctgga tggatgagcc aggcccgacc gacgtgctgt
     5641 ccttcccgat ggacgagctc cgccccggcc gcgacgaccg tgacgtcgat gagacggacg
     5701 acgcgggcat gctcggcgac gtcgtgctgt gcccggctgt ggccgcaaag cagggccacg
     5761 tcgccgggca ctcggttcag gacgagctcg aactcttgtg cacgcacggc atcctgcatc
     5821 tgctcgggtt cgaccacgcc gagcccgatg atgagaaagc gatgttcggc ctccaacgcg
     5881 atttgctcac cgcctggcag tcggcacgca cggcccagcc ggcgcagtcg acgccgaccg
     5941 cggaaagtac cggcgcatga atcccggcga cgtcctgctg cccgtcagcg tggtcgtcct
     6001 gatctttgtg gccggtgctt gcgccgctgc agacgccgcg ttgtctcgcg tctcgcgggt
     6061 gcacgtcgac gactatctac gcgacaaccg ccgaggcgct gttgcgttgc gtcgggtcat
     6121 tgcggagccg ccgcgctacc tcaacgtggt gctgttgctg cgggtcgccg ccgagatgac
     6181 tgccgccgtg atggtcgccg tctgggccat cggccggttc ggcaagagct gggacactgt
     6241 gctcgccacc gcggcggcca tggtcgtcgt cagttacgtc gtcgtcggcg tcgcgccccg
     6301 caccctcggc cgccagcacg ccgacggggt ggcgctgttc tccgcaccgt gggtcgtgcg
     6361 cctcgccggg attctcgggc cgctgccgaa gtttctcatc gcgctgggca acgccctcac
     6421 tcctggtcag ggttttcgcg acggcccgtt caccagcgaa gcagagctgc gggacatggt
     6481 cgacctcgcc gaggcgagca aggtcatcga gagcgacgaa cgccaaatga tccactcggt
     6541 gttcgaactc ggcaacacga tcgtgcgcga ggtcatggtc ccgcgaaccg acatcgtgtt
     6601 catcgagcaa accaagacgc tgcggcaggc gatgtcactc gcgttgcgca gcgggttctc
     6661 ccggctgccg gtggtcggcg agaacgaaga tgacgtcgtt ggcacagtct acctgcgtga
     6721 catcacccga cgcgtcttcg agtaccgcga atcggagtcg gccgaacgcg tcgaatcgct
     6781 gatgcggccg gcgacattcg tgccggacag caaaccggcc gacgtcctgc tacgtgagat
     6841 gcaggccgag cgcatccacc tcgtcatcgt tgtcgacgag tacggcggca ctgcgggcct
     6901 cgcgacgatc gaggacatcc tcgaagagat cgtcggtgag atcaccgacg aacacgatcg
     6961 cgagggcccg cgcatcgaac ggctccccga cggcggcgta cgggtgacgg cacggctacc
     7021 ggtcgacgag ctcgcggagc
//
LOCUS       DATCBJ010000462         3609 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_330176, whole genome shotgun sequence.
ACCESSION   DATCBJ010000462 DATCBJ010000000
VERSION     DATCBJ010000462.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 3609)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3609)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3609
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_330176"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..418)
                     /locus_tag="VII50_09355"
     CDS             complement(<1..418)
                     /locus_tag="VII50_09355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5051097.1"
                     /translation="MLLLTHSRSRAFVIHRRPVSPTDPRLPDAFVGIVAFGVTLGVAV
                     AIASAGKGFHWGTQLAFMAVAVGVVCWWCRPLPSLVVAACGWLMLNGLVVHAAGQLGW
                     SGRQDVVRIGVLVATGFAMALARSFEVDRHQLAARQG"
     gene            632..1780
                     /locus_tag="VII50_09360"
     CDS             632..1780
                     /locus_tag="VII50_09360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5051098.1"
                     /translation="MVAAAVGALAALFVAARDVTHGGHLGSFALASNVFVHPGATTDL
                     PVRTGTNGYDGQFYYRLALNPLTHQATAFHITLDNPPYRQQRIGLPATAWAVREVFRA
                     PTSLALVLVNVLALVVVGWAGATWARKYGRSPWWGVSLAASPALVMALARDLTEPLAT
                     ATLVVGLLSWTRGRLWVASLAFTAAGLCRETVLVVLLGMGVWCLVQLLRAGGRAARRT
                     SAAQIVALFVPAAVEVGWQFHVREVWGGKKLPTLSGNGQVGFSLLKPVRSFFSNASEI
                     GLSHQGILDAIWLVERFLLLGLLIVVASGLLRSAAPPEIRLGWVFAALLGLSVSWNQD
                     VEFVRAANEAIIVGQLLLLARKDRVARFAVTGIAGWSLLVAAVYAAAL"
     gene            complement(1801..2940)
                     /locus_tag="VII50_09365"
     CDS             complement(1801..2940)
                     /locus_tag="VII50_09365"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HEY5051099.1"
                     /translation="MTLRRVVPVIVFGCIVLSSCRTHTGSASPSQLPEFPSPTSPAPG
                     GETYAEILGTLPPTSGPPVITVLQGANDAAPAKTAPGQFFASGGPVTVGALRLVVTSP
                     KTGSPTLAATDTRSGREVWAYKDATEPDLLEFAVGGGVVVIGVGHEVGAAMVYAATEH
                     LDVVDLATGTPLWRSTVTGDVQSPALVAMPGLVVFESTGHAVAVHARTGVLAWTDSAQ
                     PGCPEFTPGDGGLTSLAADADVLAATFGCGSGAVTQRLNLGTGAATWTTPLYSGGQSE
                     LLAVPVNTDVVVIPEMGNGSYLEGPIRQWIPAGVPEDDTAGAVYTVLDVATGAVRWTE
                     AGAPIYNAGAYGDNAGSLCFIGAHTYECRSPKDGTLVRVGGPASP"
     gene            complement(3010..>3609)
                     /locus_tag="VII50_09370"
     CDS             complement(3010..>3609)
                     /locus_tag="VII50_09370"
                     /inference="COORDINATES: protein motif:HMM:NF012892.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporting ATPase C-terminal
                     domain-containing protein"
                     /protein_id="HEY5051100.1"
                     /translation="LPMLPSQILLNNMLYDSSQLAIPTDNVDEEQLRRPSHWDIGFIR
                     RFMIFFGPLSSVFDFVTFGVMLWVFHSGPAQFRSGWFVESLATQTLVIFAIRTRRIPF
                     FRSHPSLPLTLSALAVVAVGAALPATPLAHTLGFQPLPGGFFVALAGMVVCYLALIEV
                     GKRYFYRTAAASPGSGRHYTRRRHLRRRAAYFSTSARQA"
BASE COUNT          596 a         1199 c         1217 g          597 t
ORIGIN      
        1 ctccttgacg tgcagcaagt tggtgtcgat cgacttcgaa gcttcgggcg agagccatag
       61 caaatccggt agcgacgaga acgccgatgc ggacgacgtc ttggcgacca gaccagccga
      121 gctgaccggc cgcgtgaacg actaggccgt tcagcatcag ccatccgcac gcagccacga
      181 cgaggctcgg cagcgggcgg caccaccagc agacgacgcc gacggctact gccatgaagg
      241 cgagctgggt accccagtga aagcccttgc ctgcgctggc gattgccacc gcgacgccga
      301 gagtcacgcc gaatgccacg atgccgacga atgcgtcggg cagccgcggg tcggtggggc
      361 tgacgggccg gcggtggatg acgaaggcgc gggaccggct gtgcgtcaaa agcagcacag
      421 cgttctcctt tcgatcggtg ccgagggcga caacggattc agtgaagccc tgcacctggc
      481 gggcgacccg tttcttgatg gaaccctgac gcgggcgcac aaaaccctga cgcattcctg
      541 acgcatctgg gtctcacgac ggttgaagac cggtagcgtc cgagccatca gaggcctcga
      601 ccgtcgactc aacccggctc cggcgcccgg cgtggttgcc gcggccgtcg gtgcgctggc
      661 tgcactgttc gtcgcggcac gcgacgtcac gcacggcggg catctcggat cgttcgcgct
      721 ggcgagcaat gtcttcgtgc accccggcgc cactaccgat ctgccggtgc gcaccggcac
      781 caatggatac gacgggcagt tctactaccg gctcgcactc aatccgctga cccaccaggc
      841 gacggcgttc catatcacct tggacaaccc gccctaccgc cagcagcgaa tcgggttgcc
      901 cgccaccgcg tgggccgtgc gcgaagtgtt ccgcgccccg acgtccctcg cccttgtact
      961 cgtcaacgtc cttgcgctcg tcgtcgtcgg ctgggcagga gcgacgtggg cgcgcaagta
     1021 cggtcgctcg ccgtggtggg gtgtctctct cgcagcgtcg cccgcactgg tgatggcact
     1081 ggcccgcgac ctgacggagc cactggccac cgccacgctg gtggtgggcc tgctctcgtg
     1141 gacgcgcgga cggctgtggg tcgcgtccct cgcgttcact gccgccggcc tttgccgaga
     1201 aacagtgctc gtcgtgctgc tcggcatggg cgtgtggtgt ctcgtgcaac tgctgcgcgc
     1261 tggtggtcgc gcagcccggc gcacgagtgc tgcccagatc gttgcgctgt tcgtgccggc
     1321 cgctgtcgag gtcggttggc agttccatgt gcgcgaggtg tggggcggca agaagctccc
     1381 gacgttgtcg ggcaacgggc aggtcgggtt cagcttgttg aagccggtgc gttcgttctt
     1441 ctccaatgcc agcgagatcg gtttatcgca tcagggaatc ctcgacgcga tctggctcgt
     1501 cgagcgcttc ctgctgctag gcctactgat cgtcgtggct agcggcttgc tgcgatctgc
     1561 tgcgccacct gaaatccgac tcggctgggt gttcgctgca ctgctcggtc tgagtgtctc
     1621 gtggaatcag gacgtcgaat tcgttcgtgc tgccaacgag gccatcatcg tcgggcagtt
     1681 gctgctactc gcacgcaagg atcgcgtcgc ccgcttcgcg gtgacgggaa ttgcgggatg
     1741 gtcgctgctc gtcgccgccg tgtacgcggc ggcgctctaa cgcggtcaac cccggccagg
     1801 ctagggcgac gcgggaccgc cgacgcggac aagcgtgccg tccttgggcg accggcactc
     1861 gtacgtgtgc gccccgatga agcacaggct tcctgcgttg tcgccgtatg cccccgcgtt
     1921 gtagatgggc gcgccggcct cagtccagcg aaccgcaccg gtggccacgt cgagcaccgt
     1981 gtacacagcg cccgcggtgt catcctcggg taccccggcc gggatccatt ggcggatcgg
     2041 gccctccaag tagctgccgt tgcccatttc ggggatgacc accacgtcgg tgttgaccgg
     2101 tacggcaagc agttccgact gtccgcccga gtaaaggggt gtcgtccagg tcgcggcgcc
     2161 cgtccctagg ttgagccgct gcgtcacggc gcccgacccg cagccgaagg tcgcggccag
     2221 cacgtcggcg tccgctgcga ggctcgttaa gccgccgtcc ccaggcgtga attctgggca
     2281 ccccggctgt gcggagtcag tccacgccag gacgccggtg cgcgcgtgca ccgcgaccgc
     2341 gtgacccgtc gactcgaaga cgacaagacc aggcatggcc acaagagcag gcgattggac
     2401 gtcgccggtg accgtcgagc gccacagcgg tgtaccggtg gcgaggtcaa cgacatcgag
     2461 atgttcggtg gcggcgtaga ccatcgcagc gccaacttcg tggccaacgc cgatgaccac
     2521 gaccccgcca cccaccgcga actcgagcag gtcgggttcg gtggcgtcct tatatgccca
     2581 gacctcgcgg ccgctcctgg tgtcagtcgc cgccagcgtg gggctgccgg tcttggggga
     2641 cgtgacgacg aggcgcagcg ccccgactgt gaccggaccg ccgctggcga agaactgacc
     2701 aggtgcggtc ttcgccggcg cggcatcgtt cgcaccttgc aacaccgtga tgaccggtgg
     2761 cccggacgtc ggtggcagcg tcccgaggat ctcggcgtag gtctcgccgc cgggcgcggg
     2821 actcgtcggc gaggggaact ccggcagctg acttggtgac gcgctaccgg tgtgggtgcg
     2881 acagctcgac agcacgatgc agccaaacac aatcaccggc accacacgac ggagcgtcat
     2941 acccaccgcg actctcgagg cgatcacgct gcttcgacgg ctcacgtcgc agcttggttc
     3001 ctcctcgccc tacgcttgtc tcgcgctcgt gctgaagtac gccgcacggc ggcgcaggtg
     3061 ccggcggcgg gtgtagtgcc gtcctgaccc cggcgacgcc gcagccgttc ggtagaagta
     3121 ccgcttgccc acctcgatca gtgcgaggta gcacacgacc atgcctgcca gcgcaacgaa
     3181 gaacccaccc ggcagtggct ggaagcccag ggtatgcgcg agcggcgtcg cgggaagcgc
     3241 cgcaccgacc gccacgactg ccaacgcgga gagcgtcagc ggcaggctcg ggtgactgcg
     3301 gaagaaggga atccgccgcg tgcgaatcgc gaagatgacg agcgtttgcg tcgccagcga
     3361 ctcgacgaac caccctgagc gaaattgcgc gggcccggaa tggaagaccc acaacatgac
     3421 accgaacgtg acgaagtcga aaaccgagct cagcgggccg aagaaaatca tgaagcggcg
     3481 gatgaacccg atgtcccaat gagatggccg ccgcaactgc tcctcgtcga cgttgtccgt
     3541 tggaatcgcc agctggctgc tgtcgtagag catgttgttg agcaggatct gggacggcaa
     3601 catcggaag
//
LOCUS       DATCBJ010000463         2015 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_46828, whole genome shotgun sequence.
ACCESSION   DATCBJ010000463 DATCBJ010000000
VERSION     DATCBJ010000463.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2015)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2015)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2015
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_46828"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..919
                     /locus_tag="VII50_09375"
     CDS             <1..919
                     /locus_tag="VII50_09375"
                     /inference="COORDINATES: protein motif:HMM:TIGR02276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YncE family protein"
                     /protein_id="HEY5051101.1"
                     /translation="TGTVVKTIPVGKGPSYGVSSDGTHVWVTNNSGDTVSEIDASTGT
                     VVKTIPVGTGPLGVSSDGTHVWVVAGGAVSEIDASTGTVVKTIPVGAGPYAVSSHSNH
                     VWVTNYNGDTVSEIHVSTATPPPVLGKSVDVAPVSGVVLLKQPGKKGFVRLRAGERVP
                     VGSMLDTTAGVVSLTAALNARGKTTTGRFYAGQFRLVQKRARTAELTVLTLTGPKPTG
                     CAAGVATAARKRPKKRSLWGSASGGFATVGSDGSATELGTKWLTEDTCAGTLIRVAQG
                     AVLVDDFPHHRTFVLRAPHSFLAHRGKGG"
     gene            970..1359
                     /locus_tag="VII50_09380"
     CDS             970..1359
                     /locus_tag="VII50_09380"
                     /inference="COORDINATES: protein motif:HMM:NF023178.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3307 domain-containing protein"
                     /protein_id="HEY5051102.1"
                     /translation="MIWPAALLALLVSHAVGDVLLQTDWQAVGKVGGMGDPVARCALG
                     RHVAIYTLAFVPALVWIGSQTSAVRAVVVGALISVPHLLVDDGRLVRAWLRYVKRAPA
                     PAPALTIAVDQSLHLVCLFGAALVAAG"
     gene            1359..>2015
                     /locus_tag="VII50_09385"
     CDS             1359..>2015
                     /locus_tag="VII50_09385"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HEY5051103.1"
                     /translation="MQAARLLRNVPVLRGLSDELLERLGGEVAEVHVAAGDWIMREGD
                     AADSLFIVRSGRVDVIDEGPPEILVRVLRRGDVLGELALLRQGTRSASVRARRDTQLL
                     QLGRAAFEALIQEAPSFALGLTRAMGAQLAASRTPMAAAVPPHTIAVVGLDAAAPVAE
                     VAEGLASALANHGSVAKLSTGELATIDQAERDADRVLLRGGIAPGEEWTELCVREADL
                     V"
BASE COUNT          286 a          659 c          747 g          323 t
ORIGIN      
        1 gaccggcacg gtcgtcaaaa cgattccggt cggcaagggc ccctcctatg gggtgtcgtc
       61 ggatggcacc cacgtctggg tcacgaacaa cagcggcgat acggtcagtg agatcgatgc
      121 atcgaccggc acggtcgtca aaacgatccc ggtcggcacc ggccccttgg gggtgtcgtc
      181 ggatggcacc cacgtctggg tcgtcgccgg cggggcggtc agtgagatcg atgcgtcgac
      241 cggcaccgtc gtcaaaacga tcccggtcgg cgccggcccc tatgcggtgt cgtcgcatag
      301 caaccacgtc tgggtcacga actacaacgg cgataccgtc agtgagatcc acgtgtcgac
      361 ggcaactccc ccgccggtgt tgggtaagag tgtggatgtg gcgccggtgt cgggggtggt
      421 gttgctcaag cagcctggta agaagggttt tgttcgtttg cgggctggtg agcgggtccc
      481 ggtcggcagc atgctcgaca cgacggcggg ggtggtgtcg ttgactgctg cgctgaacgc
      541 cagggggaag accacgaccg ggcggttcta cgccggtcag tttcgcctcg tccagaagcg
      601 cgcccggacg gccgagctga ccgtcctgac gttgaccggg ccgaagccga ccggatgcgc
      661 cgcgggcgtc gcgaccgccg cgcgtaagcg tccgaagaag cgcagcctgt ggggcagcgc
      721 ctcgggcggc ttcgccacgg tcggctcgga cggttcggcg accgagctcg ggacgaagtg
      781 gttgaccgag gacacgtgcg cggggacgct gatcagggtc gcccagggag cggtgctcgt
      841 tgatgacttc ccccaccacc gcacgttcgt cctcagagcc ccgcacagct ttctcgcgca
      901 tcgcggcaag ggcggctaag aggccatcat gagaacgcgg ccaaaggacc cggcggatct
      961 gccggcagca tgatctggcc cgcggccctg ctagcgctgc tggtctcaca cgcggtgggc
     1021 gacgtgctgc tgcagactga ctggcaggcg gttggcaagg tgggcgggat gggtgacccc
     1081 gttgcccggt gtgcgctcgg gcgtcacgtc gcgatctaca cgctggcgtt cgtgcccgcg
     1141 ctcgtgtgga tcggcagcca gaccagcgcc gtgcgcgcgg tcgtcgtggg tgcgctgatc
     1201 tcggttcctc atctgctcgt cgatgacggt cgcctggtgc gcgcctggtt gcgctacgtc
     1261 aagcgcgccc ccgcgccggc gcccgcgctc acgatcgccg tggaccagag cctccatctc
     1321 gtctgcctat tcggcgcggc gctggtggcc gcgggataat gcaggccgcc cggcttctgc
     1381 gcaacgttcc ggtccttcgg gggctgtcgg acgagctgct cgagcgcctt ggcggggagg
     1441 tcgcggaggt ccacgtggcc gcaggcgact ggatcatgcg cgaaggcgac gcggccgaca
     1501 gtttgttcat cgtgcgcagc ggccgggtcg acgtgatcga cgaggggccg cccgagatac
     1561 tcgtgcgcgt tctgcgccgc ggcgacgtgc tgggcgagct ggcgctgctg cggcagggta
     1621 cgcgctcggc gtcggtgcgc gcccggcgcg acactcagct gctacagctg ggccgagccg
     1681 cgttcgaagc gctgatccag gaggcgccga gcttcgcgct cggcctcact cgagcgatgg
     1741 gggcgcagct cgcggctagc cgcacgccga tggccgcggc cgtaccaccg cacacgatcg
     1801 ccgttgtcgg cctcgacgcc gcggcgcctg tcgcggaagt agccgaaggg ctcgccagcg
     1861 cgctcgcgaa ccacgggtcg gtggccaaat tgagcaccgg cgagctcgcc acgatcgacc
     1921 aggccgagcg cgacgccgac cgggtgctcc tgcgcggcgg catcgcgccc ggcgaggagt
     1981 ggaccgagtt gtgcgtgcgc gaagccgacc tcgtc
//
LOCUS       DATCBJ010000464         2627 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_207601, whole genome shotgun sequence.
ACCESSION   DATCBJ010000464 DATCBJ010000000
VERSION     DATCBJ010000464.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2627)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2627)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2627
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_207601"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..829
                     /locus_tag="VII50_09390"
     CDS             <1..829
                     /locus_tag="VII50_09390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018555222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HEY5051104.1"
                     /translation="LLDGDAAKLAQLEASVVGHLGFERVLNSVGQVYPRSLDFDVVTA
                     LVQATAGPSSLATTIRLMAGHELVTEGFKAGQVGSSAMPHKMNTRSCERVNGLAVVLR
                     GFAGMTGELAGDQWNEGDVSCSVVRRVALPSAFFAADGLFQTFLTVLDEFGTYPAVVQ
                     RELDRYLPFLATTKVLIAAVRHGVGREVAHEAIKEHAIAVALAMREKGIDHNDLFERL
                     AADDRLQLTDADIASLLSSPLAFTGAATAQVAAFCDQVALVTARYPDAAFYHPADIL"
     gene            897..1781
                     /locus_tag="VII50_09395"
     CDS             897..1781
                     /locus_tag="VII50_09395"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009084818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HEY5051105.1"
                     /translation="MSDYTFIASGKVRDLYEMDDGSLLFVASDRISAYDFVLPTPIPD
                     KGAVLTAMSIWWFEQLADVVPNHLISTDVPAEFAGRAMICERLDMLPVECIARGYLTG
                     SGLSDYKRTGAVSGIPLPVGLVDGSRLPQPLFTPSTKAPIGEHDENISLADVEDTIGA
                     EAADELKRLTLAAYGRAEEIARGRGIILADTKFEFGRRGTRGPHGVQIAGGEIVLADE
                     VLTPDSSRFWPADLYEPGHAQPSYDKQYVRDWLTSPASGWDKSSDMPPPPLPDDVVAK
                     TREKYIEAYERLTGLAWS"
     gene            1800..2306
                     /locus_tag="VII50_09400"
     CDS             1800..2306
                     /locus_tag="VII50_09400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5051106.1"
                     /translation="MAAILAAVKKQAHTAREQIAAHVRADESLLCVFAVTLPRPSAGT
                     AWGDAATVVPLVTIVSKVMWNRTAAAAQHKVGIGVRTRMVWALTDQRILLFSALKRWQ
                     LGEWLDEIPRVEVTSATAPTMGQGWRTVIIELRASRKLVIKVGPTLADDLAAELTPAT
                     PSGTNLDG"
     gene            2299..>2627
                     /locus_tag="VII50_09405"
     CDS             2299..>2627
                     /locus_tag="VII50_09405"
                     /inference="COORDINATES: protein motif:HMM:NF016152.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HEY5051107.1"
                     /translation="MADADDVRRLALSLPQVVEIPSEGFDFRVGNKGFVWSYPEYRSG
                     RRREIRTDIAVLYVGDEAEKQALVLGEPALFFTTPGYDGFPLVMLRLDEVDVERLTEL
                     VTDAWRMR"
BASE COUNT          468 a          883 c          830 g          446 t
ORIGIN      
        1 tttgctggac ggcgatgcgg cgaaactcgc ccaactcgaa gcgagcgtcg tgggccacct
       61 gggtttcgag cgggtactga acagcgtcgg ccaggtgtat ccgcggtcgc tggacttcga
      121 cgtggtcact gcgctcgttc aagcaactgc cggaccctcg agcctcgcga ccaccatccg
      181 cctgatggcc ggtcacgagc tggtgaccga gggcttcaag gccgggcagg tcggctcgag
      241 tgcgatgccg cacaagatga acacccggtc gtgcgagcgg gtgaacggtc tcgccgtcgt
      301 cctgcgcggc ttcgcgggca tgaccggcga gctcgccggc gaccagtgga acgagggcga
      361 cgtttcgtgc tcggtcgttc gacgcgtcgc gctacccagc gctttcttcg cagcagatgg
      421 acttttccag acgttcctga cagttctcga cgagttcgga acctatccag ccgtcgtgca
      481 gcgcgagctc gaccgctacc tgcctttcct ggccacaacc aaggtcctga tcgccgcggt
      541 ccggcacggg gttggtcgcg aagtggcgca cgaggccatc aaggagcacg cgatcgcagt
      601 cgcgttggcg atgcgcgaga aaggcatcga ccacaacgac ctgttcgagc gcctcgccgc
      661 cgatgaccgg ctacagctca ctgacgccga cattgcctcg ctgctgtcgt ctccgctcgc
      721 gttcaccggc gccgcgaccg cgcaggtggc cgcgttctgc gatcaggtcg cgctcgtcac
      781 ggcccgttat cccgacgcgg cgttctacca cccagccgac atcctctgac gagaccgcct
      841 tacaaccccg atacgcgctc tacaccctcg ctacgcggca tggcacactt cggcgaatga
      901 gcgactacac cttcatcgcc tcgggcaaag tacgtgacct ctacgagatg gacgacggca
      961 gcttgctgtt cgtcgccagt gaccgcatct cagcctacga tttcgtactc ccgacaccga
     1021 tccccgacaa gggcgccgtc ttgacggcca tgtcgatctg gtggttcgag cagctcgccg
     1081 acgtggtgcc caaccacctg atctcgaccg acgtgccggc ggagttcgcc gggcgagcaa
     1141 tgatctgcga gcgcctcgac atgctgccgg tcgaatgcat cgcgcgcgga tacctgacag
     1201 gttcgggatt gtcggattac aaacgcaccg gtgccgtctc cgggatcccg cttcccgtgg
     1261 gcctggtcga cgggtcgcga ttgccgcaac cgttgttcac gccgtccacc aaggcgccga
     1321 tcggcgagca tgacgagaac atctcgctcg ccgacgtcga ggacacgatc ggcgccgagg
     1381 ccgctgacga actcaagcgc ctcacgctgg ctgcgtacgg tcgcgccgag gagatcgcgc
     1441 gcggccgcgg catcatcctg gccgacacaa aattcgagtt cggacggcgt ggcacccgcg
     1501 gcccccacgg tgtgcagatt gcgggcggtg aaattgtcct ggctgatgag gtgctcactc
     1561 ccgactcgtc gcgcttctgg ccggccgacc tctacgaacc tggccacgcg cagccgtcgt
     1621 acgacaagca atacgtgcgc gactggctga cctcgccggc atccggctgg gacaagagct
     1681 cggacatgcc gccaccgccg ttgcctgacg acgtcgtggc gaaaacgcga gagaagtaca
     1741 tcgaggcgta cgagcggctc accggcctcg cctggtccta gccgccagaa ctggcggacg
     1801 tggccgcaat acttgcggcc gtgaagaagc aggcccacac agctcgcgag caaatagccg
     1861 cccacgtgcg agcggacgag tcactcctgt gcgtcttcgc cgtgaccctt ccacgcccga
     1921 gcgccggcac tgcgtggggc gacgcggcca ccgtcgtgcc gttggtcacg atcgttagca
     1981 aggtcatgtg gaaccgaaca gccgcggccg cccaacataa ggtgggaatc ggcgtgcgca
     2041 cgcgcatggt gtgggcgctt acagatcaac gaattctgct cttcagcgct ctaaagcgat
     2101 ggcagctcgg tgagtggctc gatgagatac cgagagtgga ggtcaccagc gctaccgcac
     2161 cgacaatggg ccagggctgg cggacggtca tcatcgagct acgagcgagc cggaaactcg
     2221 tgatcaaagt tggcccgacg ctagctgacg acctagctgc cgagctcacg cctgccacgc
     2281 cctccgggac caatctcgat ggctgacgcc gacgacgtac gccgcctcgc gttgagcctg
     2341 ccgcaggttg tcgaaatccc gagcgaaggc ttcgacttcc gggtgggcaa caagggcttc
     2401 gtctggtcct accccgaata ccgttccggg cggcggcgcg aaattcggac cgatatcgcc
     2461 gtactgtacg tgggcgacga ggccgagaaa caggcactcg tgctcggcga accggcgctg
     2521 ttcttcacca cacccggcta tgacggattc ccgctggtga tgctccggct cgacgaagtc
     2581 gacgtcgaac gcctcactga actggtgacc gacgcctggc ggatgcg
//
LOCUS       DATCBJ010000465         2709 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_378612, whole genome shotgun sequence.
ACCESSION   DATCBJ010000465 DATCBJ010000000
VERSION     DATCBJ010000465.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2709)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2709)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2709
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_378612"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            288..1307
                     /locus_tag="VII50_09410"
     CDS             288..1307
                     /locus_tag="VII50_09410"
                     /inference="COORDINATES: protein motif:HMM:NF012742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEY5051108.1"
                     /translation="MATTIHDVAARAGVSTATVSRALRGFPNVSDGARARVQEAARQL
                     DYVVSVAASGLASGRTRTIAVAAPFVGRWFFGEVVDGIEDVLRTGDMDMLLYNVGDTR
                     RRNRFFADLPIRRRVDGLIVLSLPLSSDELAALRPLAVPVVLVGLRADGFSSVRIDDV
                     AAAVTATQHLINLGHVRIALLSDSRYETIPFKNTAERRWGYRTALLNAGLTPEPALEV
                     SGPFGVDGGSRAMADLLGAPSPPTAVFAEFDEMAYGAIRTLRRMRMRIPEDMSVIGVD
                     DHELSELMDLTTVRQPVREQGSQAARLLLQLLDQPQQQAEEIVLPTQLVVRGTTSICR
                     GADNR"
     gene            complement(1564..>2709)
                     /locus_tag="VII50_09415"
     CDS             complement(1564..>2709)
                     /locus_tag="VII50_09415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY5051109.1"
                     /translation="NKSWAQFESCTGIKIAYEGSNSFESQLPVRVQGGTAPNLAIIPQ
                     PGLLAQMVALGAVKAPPAGVVANEDKYFNAAWKSYGSVSGTFYAAPQSANMKSLVWYS
                     PSAMSKAGYTIPTTWDQLAALSDKIAASGTKPWCGGIGSGTATGWPATDWLEETVLSS
                     QGGQVYDDWVSHKIKFSDPQIQTAMTDVANWMQNPKWVNGGYGDVKTIATTTFQDAGK
                     PILTGQCWFLQQASFYEAQWPKGTTVSPTGQVWAFYLPPRSTSVPTPVEGGGEFTAAF
                     SSDPATQAVQTYLSSPQWADSRIQVAPGWVSANSGVDQSLYTDGIDKLSAQFLTNSKA
                     TFRFDASDLMPSAVGSGAEWTQMTAWFGTGASQVSVAKAIDAAWPAS"
BASE COUNT          486 a          833 c          873 g          517 t
ORIGIN      
        1 gagcggtccg tccagtaccg acagcagtcc actaagactg ccaccgagcc gatcggcgta
       61 ggtcgcgagt tgcaccgtgc gcctcaattg tgcagtcctt cctatcgagc ggctgcaacc
      121 gtttgctgcc gaggggtcaa attccagacg gttttaggcc gcccggatac aagctctccg
      181 agcgtgagcg tgattctgag acgatctcgt gtctggcaaa cgcttgcacg agggactgta
      241 caggcttagc gtgacgtgta caccccggag cgggtcgagg aagtgaaatg gcaacgacaa
      301 ttcacgatgt cgccgcgcgg gcgggggtgt cgaccgcgac cgtgtctcga gcgttacgag
      361 ggttcccgaa cgtctcagat ggcgcgcggg cgcgcgttca ggaagccgcg cggcaactcg
      421 attacgtcgt gtcggtagct gcctcggggc tggcgtctgg tcgtacgagg acgattgcgg
      481 tagctgcacc gttcgtcggc cgctggtttt ttggcgaggt agtcgacgga atcgaggacg
      541 tccttcgtac tggcgacatg gacatgctct tgtataacgt cggcgacaca cgtcgacgca
      601 acaggttctt cgctgatctt ccgatacggc gccgtgtcga cggcctgatc gtgttgtcat
      661 tgcccctgtc atcggacgaa ctggcggcgc tgcgcccgtt ggcggtgccg gtcgtgctcg
      721 taggcctgcg tgcagacgga ttttcgagcg tccgcattga cgacgttgca gcagcagtaa
      781 cggctaccca acacctgata aacctgggtc acgtgcggat cgcgctactc agcgatagcc
      841 ggtacgaaac gatcccgttt aagaacaccg cagaaagacg ctggggttat cggacggcgc
      901 tgctgaacgc cgggttgacc ccggagccag ccctcgaagt atcgggtccg ttcggggtcg
      961 acggtgggtc cagggcaatg gccgacctgt tgggcgcgcc gagtccgccg acagcggtgt
     1021 tcgccgagtt cgatgagatg gcctacggcg ctatccgaac gctgagacga atgcggatgc
     1081 gtattcctga ggacatgtcg gtaattggag tcgatgacca tgagttgtct gagctgatgg
     1141 acttgacgac cgttcgacaa cctgtacgcg agcagggcag tcaggccgcc cgtctgctgc
     1201 tgcaactcct cgaccagcca cagcaacaag ccgaggagat tgtgttaccg acgcaactcg
     1261 tcgtgcgggg caccacgagc atctgtcgcg gagcagacaa tcgctgacag atcctgaatg
     1321 cgcgggggat cccaaactgt gaccgcgcaa gggacagttc ggtatcgggt gactgccctc
     1381 agccgcgtcc ttggactgac ttatcgccgt ccctgtagcg aagcgaccgg ttctgcgcca
     1441 ttacgcgccg ggtgcgcacg tgccccggcc ctccgccaaa tggccgacgg gaagcgccgt
     1501 ggcccggagg tctctccttc cgggccacgg cgccgtctta gcgggacatt ctctccggcg
     1561 gtgttacgag gctggccacg cagcgtcgat ggccttcgcc accgacacct gtgatgcgcc
     1621 cgtgccgaac caggcagtca tctgcgtcca ctcagcaccc gatccgacag ccgacggcat
     1681 caggtccgat gcgtcgaagc ggaacgttgc cttcgagttg gtgaggaact gcgcggacaa
     1741 cttgtcgatg ccatcggtgt acaggctctg gtcgactccg ctgttcgcgg aaacccagcc
     1801 tggtgcgacc tggatccggc tgtcggccca ctgcggtgac gacaggtacg tctgcacggc
     1861 ctgggtcgcc gggtcggagg agaacgcagc ggtgaactcg ccgccacctt cgaccggggt
     1921 cggaacgctc gtgctacgcg gcggcaggta gaaggcccac acctgaccgg tcggagagac
     1981 cgtggtgccc ttcggccact gtgcctcgta gaacgacgcc tgctgcaaga accagcactg
     2041 accagtgagg atcggcttgc cggcgtcctg gaacgtcgtc gtcgcgatcg tcttgacgtc
     2101 gccgtatccg ccgttgaccc acttcgggtt ctgcatccag ttcgcaacgt cggtcatcgc
     2161 cgtctgaatc tgcgggtcag agaacttgat cttgtgactc acccagtcgt cgtaaacctg
     2221 accaccttgg gagctgagca cggtctcttc gagccagtca gtggccggcc agccggtcgc
     2281 cgtacccgat ccgataccgc cacaccacgg cttggtgccg ctggccgcga tcttgtcaga
     2341 cagtgcggcg agctgatccc acgtcgtcgg gatcgtgtag ccggccttgg acatagccga
     2401 cggcgagtac cacacgagcg acttcatgtt cgcgctctgg ggtgccgcat agaacgtgcc
     2461 gctgacggag ccatacgact tccacgcagc gttgaaatac ttgtcttcgt tggccactac
     2521 cccggccggc ggagccttga cggcgccaag agcaaccatc tgagcaagca agccgggctg
     2581 cggaatgatg gcgaggttag gtgctgtgcc gccctgaacg cgaacgggca gctgcgactc
     2641 gaaggagttc gagccctcgt aggcgatctt gatgccagtg cagctctcga actgcgccca
     2701 cgacttgtt
//
LOCUS       DATCBJ010000466         3394 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_374446, whole genome shotgun sequence.
ACCESSION   DATCBJ010000466 DATCBJ010000000
VERSION     DATCBJ010000466.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 3394)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3394)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3394
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_374446"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1224
                     /locus_tag="VII50_09420"
     CDS             <1..1224
                     /locus_tag="VII50_09420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY5051110.1"
                     /translation="DAALAVATGGMGLQILGDFAGVIRIAEPRWQALDGVAGAERALL
                     QLARPLANATGQLENEAATRGYVERRVLLAEAVGDAGALAESLVQLGTHYHGIGGPAT
                     ARALYEAAADISRANDVPSTLARSLINIGTVLMSRDLPAAIDALREARDVARRSGVVA
                     MIEFAEGNYASTLWLAGRLSDSRTIAGEAAATAATPSMQSLLACIQANIADATGEPMP
                     VPPHAAGTGGAWDRAGFGYLEMRRLLASGDAVAAAATTESTLVHVLAAGQLDDDFCWL
                     WPPLVQAAVMAGDAPLAMRLLEPVTTASDGIISAAVAAQFRYLLGLVGALRGDDSGQV
                     EADLRAGVAALADFGAVGASARAEEDLARWLIGQGRAQDARPHLEHARGVYEWMGATG
                     WLRALDAQVSASLAP"
     gene            1320..1673
                     /locus_tag="VII50_09425"
     CDS             1320..1673
                     /locus_tag="VII50_09425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5051111.1"
                     /translation="MSALQAKLWHVTVTVGGDAMETALVRAALERLSGEQPFLLSGRY
                     SNDKAEVRYWEEAPECADAAALALRLWGEHRATAGLPLWHVLGIEVVDREVFQRRGGD
                     AAVSLVAPAGGWRPF"
     gene            1698..2969
                     /locus_tag="VII50_09430"
     CDS             1698..2969
                     /locus_tag="VII50_09430"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009056334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HEY5051112.1"
                     /translation="MGTDTGPDTAADVLATAQRARVASTKLATASRAVKDAALLAIAA
                     ALVARVDEVLSANKVDVDAAATSGATDSYIDRLRLDGPRVEAIAADVVKTAGLPDPVG
                     EVLRGGVLANGLELRQIRVPLGVVGIIYEGRPNVTVDAAALCLKSGNAVLLRGSSSAL
                     SSNQALVAILRDAVASVDLPADAIQLVPGAGHEAAKHLMRARGLVDVLIPRGGAGLIR
                     SVVEESTVPVIETGVGNCHVYVDADADLDMAITILLNAKTSRPSTCNTAETFLVHAAV
                     ADAFLPRALAALHAAGVTVHGDAAVAALDPAVVPATDADWDTEYLSLDIAAAVVPSID
                     AAIAHIRNHGTAHSEAIVTRSQAAARTFIAEVDAAAVLVNASTRFVDGGEFGFGAEIG
                     ISTQKLHARGPMGLPELTSTKYVVVGDGHLR"
     gene            2980..>3394
                     /gene="nadD"
                     /locus_tag="VII50_09435"
     CDS             2980..>3394
                     /gene="nadD"
                     /locus_tag="VII50_09435"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HEY5051113.1"
                     /translation="MSAPTPSNGPRVGVMGGTFDPIHHGHLVAASEVASKFGLDEVIF
                     VPAGDPWQKADREVSPAEDRYLMTVIATASNPRFSVSRIEIDRPGPTYTKDTLIQLRD
                     ELTVSRAAVPALFFITGADALAQILSWHNAEMLPDL"
BASE COUNT          513 a         1109 c         1187 g          585 t
ORIGIN      
        1 gatgcggcgc tcgccgtggc gaccggcggc atgggtctcc agatacttgg cgattttgcg
       61 ggagtcatcc gcatcgccga accgcgatgg caggcgctcg acggcgtcgc gggagccgag
      121 cgggcgttgc tccagctggc gaggccgctc gcgaacgcca ccggacagct cgagaacgag
      181 gcggccacca gggggtatgt ggaacggcgg gtgctactcg cggaggcggt aggagacgcc
      241 ggcgcgctcg ccgagtcgtt ggttcaactg ggcacccatt atcacggcat cggcgggccg
      301 gccacggctc gagcactcta cgaggctgcc gccgacatct ctcgggcgaa cgatgtgcca
      361 agcacgctcg cgcgttcgtt gatcaacatc ggcacggttc tcatgagccg tgaccttccg
      421 gcggccatcg atgcgctccg ggaagcgcgc gacgttgccc gccgttcggg cgtcgtcgcg
      481 atgatcgagt ttgcggaagg caactatgcg tcgaccctct ggctcgccgg tcggctgagc
      541 gattcgcgaa ccattgccgg ggaggcggcc gcaaccgcgg caaccccgtc gatgcagagc
      601 cttctcgcgt gtatccaggc caacatcgct gacgcgacag gcgagccgat gccagttccg
      661 ccgcatgcgg ctggtacggg tggcgcgtgg gatcgcgctg ggttcggata cctcgagatg
      721 cgacggctgc tcgccagcgg cgacgcggtc gccgcagctg cgacgacgga gtcgacactc
      781 gtccacgtgc tggccgctgg acaactcgac gacgactttt gctggctatg gccgccgctc
      841 gtacaagcgg ccgtaatggc gggcgacgca ccgctggcga tgcgtctcct cgaaccggtc
      901 acgaccgcat ctgatggcat catctccgcg gcagtcgccg cccagtttcg gtatctgctt
      961 gggttggtcg gggcgttgcg tggcgatgac tccgggcagg ttgaggccga ccttcgggcc
     1021 ggcgtagccg cactcgccga cttcggcgct gtgggtgcaa gcgcccgcgc tgaggaggat
     1081 ttggcacgct ggctgatcgg gcaaggccgc gcccaggacg cgcgtccgca cctcgagcac
     1141 gcccgtgggg tctatgagtg gatgggtgcc accggctggc tgcgcgcgct ggacgcacag
     1201 gtatcggcga gcctcgcgcc gtagggccag aaggaccgac ttcggcaaaa cgcctccgcc
     1261 cgacctcgtg tgctgatcaa ctatgactcg tcgctgatgg gtgggaccga cgactgggga
     1321 tgagcgcgct gcaggcgaag ttgtggcacg tcactgtcac ggtgggcggc gacgccatgg
     1381 agaccgcact cgtcagggcc gcgctcgagc ggctctcggg cgagcagccc ttcttactct
     1441 ccggccggta cagcaatgac aaggccgagg ttcggtattg ggaagaggct cccgagtgtg
     1501 ccgacgccgc cgcgctcgcg ttgcggctgt ggggcgagca ccgggcgacc gctggtttgc
     1561 cgctgtggca cgtgctcggg attgaggtcg tcgaccgcga ggtgttccag cgtcgtggcg
     1621 gcgatgcggc tgtctcactc gtcgcacccg ctggcggctg gcggcccttc tagcccggtt
     1681 cggcatatcc tgacgccgtg ggcactgaca ctggacctga cactgctgcc gacgtgctcg
     1741 cgaccgcgca acgcgcgcgg gtcgcatcga ccaagctcgc caccgcatcg cgtgcggtga
     1801 aggacgccgc gctgctcgcc atcgccgctg cgttggtggc gcgcgtcgac gaggtgcttt
     1861 ccgccaacaa ggtggacgtc gatgcagcgg cgacatcggg tgctaccgac tcgtacattg
     1921 atcggctgcg acttgacggg ccgcgggtcg aggcgatcgc cgccgacgtg gtcaagaccg
     1981 cagggctgcc ggatccggtc ggcgaggttc tgcgcggcgg ggtactcgcg aatggcctcg
     2041 agctgcgtca aatccgcgtc ccgctcggcg tcgtcggcat catctacgag ggccggccga
     2101 acgtcaccgt cgatgccgcg gcgttgtgct tgaagagcgg caatgctgtg ctgctgcgcg
     2161 gatcgtcgtc cgccttgtcg tcgaaccagg cgttggtcgc gatcctgcgc gacgccgtcg
     2221 cgtccgtcga tctgcctgcc gatgcgatcc aactcgttcc gggcgcgggt cacgaagcgg
     2281 ccaagcatct gatgcgtgcc cgagggctcg tcgatgtgct gattccgcgc ggtggcgcag
     2341 gtctgatccg cagtgtcgtt gaggagtcga cggtgccggt gatcgaaacg ggcgtcggca
     2401 actgccacgt ctacgtcgac gccgacgccg atctcgatat ggcgatcacg attttgctca
     2461 acgccaagac atcgagaccc agcacgtgca acaccgccga gaccttcctg gtgcacgccg
     2521 ccgtcgccga cgcgttcttg ccgcgagcgc tcgcggctct acatgcggca ggtgtcacgg
     2581 tgcatggcga tgcagccgtc gctgcgctcg atccggccgt ggtgcctgcg accgatgccg
     2641 actgggacac cgaatacctc tcgctcgaca tcgcagcggc cgtcgtgccg tcgattgacg
     2701 cggccatcgc gcacatcagg aaccacggca ccgcgcacag cgaggcgatc gtgacccgct
     2761 cgcaagcggc tgcccggact ttcattgccg aagtcgatgc ggcggccgtg ctggtcaatg
     2821 catcgacgcg attcgtcgac ggcggcgagt tcggattcgg cgcggagatc ggcatctcga
     2881 cgcagaagct gcatgcgcga ggtccgatgg gactgcccga gctcacgtcg acgaagtacg
     2941 tcgtggtagg cgacggacac ctccgttgag cccacgcggg tgagcgcgcc aacgccctcg
     3001 aatggccctc gggtcggggt gatgggcggc acgttcgatc cgatccacca cggtcacttg
     3061 gtcgcggcga gcgaggttgc cagcaagttc ggcctcgacg aagtgatctt cgtgccggcc
     3121 ggcgatccgt ggcagaaggc cgaccgcgag gtctccccgg ccgaggatcg ctacctcatg
     3181 accgtcatcg cgaccgcgtc caatccgagg ttctccgtca gccggatcga gatcgatcgg
     3241 cccggcccga cgtacaccaa ggacacgctg atccagttgc gcgacgagct gaccgtgtcg
     3301 cgtgccgcgg tcccggcgct ttttttcatt accggtgccg atgcgttggc ccagatcctg
     3361 tcctggcaca atgcggagat gctgcctgat ttag
//
LOCUS       DATCBJ010000467         3475 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_111135, whole genome shotgun sequence.
ACCESSION   DATCBJ010000467 DATCBJ010000000
VERSION     DATCBJ010000467.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 3475)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3475)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3475
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_111135"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..488
                     /locus_tag="VII50_09440"
     CDS             <1..488
                     /locus_tag="VII50_09440"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HEY5051114.1"
                     /translation="EPRKNVPTLLEAFAELRRTDPELGLVLVGPTGWGHVRVHTQLLR
                     GVRLLGFLPADHLHAAYAGARAFCYPSLREGFGLPVLEAMAHAVPVVTSRGTAMAEFV
                     GDGGLLVDPLDANRIAAALEIVLGERHDSFATAALAQSTRYSWDNAAALTVEAYRAAA
                     S"
     gene            485..1633
                     /locus_tag="VII50_09445"
     CDS             485..1633
                     /locus_tag="VII50_09445"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HEY5051115.1"
                     /translation="MTLTVGLNLLWLVPGVVGGSEGYATGLLAPLVERDDVDVVAFGL
                     PGFVKEYPSLAAAAHTVVPPLPEGRHVMRRVLIENAWLPRHLRAQKVDLVHHLGGIVP
                     PGCRVPAVVTLHDLQYLSYPAYFSPAKRRYLGMTQGRSLKRAEVVTAVSDFTRTQAIE
                     AFDLDAAKVVVVPPVVRPMPPVSDDRRAELLGELGVRGAYIVYPATTYPHKNHAVLLE
                     AFAEVVQTHDVSLVLTGAVGAGAWGSAHSTRAEVADLVAQLGINERVRMLGYVTAEQL
                     VALYAGASLLAFPSRFEGFGIPVVEAMAAGCPVVAADATALPSLVGDAGVLVDPDDVS
                     GWAHAISELLADDSARQRLSDAGRARAATLAALDPVQLLVDVYARVGT"
     gene            1630..2856
                     /locus_tag="VII50_09450"
     CDS             1630..2856
                     /locus_tag="VII50_09450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEY5051116.1"
                     /translation="MRILAVLPHFDPDVAPTGVIASRLVEELGALGHHIDVVTSLPWY
                     EHHDVLPAWRGKMLRRERTSWGRVTRVHPFPTGDKRSIPKRAVGFAAFCALDAAVAAV
                     GGRVDVTFAMTPPLPMAATGWFAALARRAPLVLNVQDVFPDVAVELGLLRGRALISAA
                     SALERWSYARCAAVTVLSADMRANLAAKMRRPHRLRVIPNFVDVELVQPGPTENSYRE
                     EYGLSGKTVVMYAGNVGMSQSLDLLVHAAQSTRDRDDIVYVINGGGSTLPDLQAQATD
                     LPNLRFIPMQPLDRVPELLAAADLHVVPLKAGLARSSVPSKTYSIMAAGRPLVASVDG
                     GSEVQRVVEAAGAGIAVPPDDADAFAAAVLKLADDAGAREKMGAAARAYVERTASPRG
                     VAQAYADLFESVVRHK"
     gene            complement(2891..3355)
                     /locus_tag="VII50_09455"
     CDS             complement(2891..3355)
                     /locus_tag="VII50_09455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5051117.1"
                     /translation="MSKDKVPGAAQREAVESLASRLYDDVTVTEVIRPAAVLSERNAR
                     RVLDGLRAEDVRSGGRWHAEPGCWRLFERPWKGKDERSAAAAGLIGSIQVAYGMPTRW
                     EITIFRATITVVGSRTGYTVDSLCNEALRYADLTLSNCPRIDMPAPPRAFRG"
BASE COUNT          517 a         1146 c         1206 g          606 t
ORIGIN      
        1 tcgaaccgcg caagaacgtg ccgacgctgc tcgaggcatt cgccgaactg cgccgtaccg
       61 atcccgagct tggactcgta ctggtcggcc cgacgggttg gggtcacgtg cgggtgcaca
      121 cgcagttgct gcggggggtt cgactgctcg gcttcctgcc cgccgatcac ctgcacgccg
      181 cctacgcggg cgcgcgagcg ttttgctacc cgagcctgcg tgagggcttc gggttgccgg
      241 tgctggaggc gatggcgcac gcggtacccg tcgtcacctc acgcggcacc gcgatggccg
      301 agttcgtcgg tgacgggggc ctcctcgtgg acccgctcga cgccaatagg atcgccgccg
      361 ccctcgagat cgtgctcggt gaacgacacg attcgttcgc gacagcagcg ctcgcgcagt
      421 cgacgcggta ttcgtgggac aacgccgcgg cactcaccgt cgaggcctac cgggcggccg
      481 cgtcatgacg ctgaccgtcg gcctcaatct gctgtggctg gtgccggggg tcgtcggcgg
      541 cagcgaaggc tacgcgaccg gcctgctcgc gccattggtt gagcgcgacg atgtcgacgt
      601 ggtcgcgttc gggttgccag ggttcgtgaa ggagtacccc tcgctggccg cggcggcgca
      661 caccgtcgtc ccgccgttgc ctgaaggacg tcacgtgatg aggcgcgtgc tcatcgagaa
      721 tgcctggctg ccaaggcatt tgcgggccca gaaggtcgat ctggtgcacc atctgggcgg
      781 gatcgtgcca ccgggctgtc gcgtgccggc ggtcgtcaca ctgcacgatc tgcagtacct
      841 gtcatacccg gcatacttca gccccgcgaa gcggcgctat ctcggcatga cgcaaggtcg
      901 ctcgttgaag cgtgccgagg tcgtgactgc agtcagcgac ttcacccgga cacaggcgat
      961 cgaggcgttc gacctcgacg ccgccaaggt cgtcgtcgtg ccaccagtgg ttcggccgat
     1021 gcctccagta tccgacgatc gacgtgcaga gttgttgggt gagctcgggg tgcgcggggc
     1081 ctacatcgtc tatcccgcga caacctatcc gcacaagaat cacgcggtct tgctcgaggc
     1141 attcgccgag gttgtgcaga cccacgacgt gtctttggtt ctgactggag cggtcggcgc
     1201 aggcgcctgg ggctcggcac actcgacgcg agccgaggtg gccgatctgg tggcacagct
     1261 gggcatcaac gagcgggtcc gcatgcttgg ctacgtgacg gcagaacagc ttgtcgccct
     1321 gtacgcgggc gcgtcgctac tcgcgtttcc ctcccgtttc gagggtttcg gcataccggt
     1381 cgtcgaagca atggccgcgg ggtgtccggt ggtcgcggcc gacgcgaccg cgttgccgtc
     1441 gttggtcggt gacgccggcg ttctggtcga tcccgacgac gtgtccggtt gggctcatgc
     1501 gatctccgag ctgctcgccg acgacagtgc gcgacaacgg ctgtccgacg ccggccgagc
     1561 acgagcggcg acgctggcag ccctagaccc ggtgcaactc ctagttgatg tctacgcaag
     1621 ggtcggcacg tgaggatcct cgcggtactg ccgcatttcg atcccgacgt cgcgccgacg
     1681 ggtgttatcg caagtcgcct ggtcgaagag ctcggagcac tcggccacca catcgacgtc
     1741 gtgacatcac tgccgtggta cgaacaccac gatgtgcttc cggcgtggcg cggaaagatg
     1801 ctgcgtcgcg agcgcacctc gtggggtcgc gtgacacgcg tgcatccgtt cccgaccggc
     1861 gacaagcgga gcatccccaa gagggcggtc ggtttcgccg cgttctgcgc actcgacgcc
     1921 gcggtggccg ctgtcggcgg tcgggtcgat gtcacgttcg cgatgacgcc gccgctgccg
     1981 atggccgcga caggatggtt cgccgccttg gcgcgacgcg cgccgctcgt gctcaacgtg
     2041 caagacgttt ttcccgacgt cgcggtcgag ctcggcttgc tccgcggccg cgccctcatc
     2101 tcggcggcga gcgcgctgga gcgttggtcc tacgcgcggt gtgcggcagt caccgtgctg
     2161 tcggccgaca tgcgggcgaa cctggcggcg aaaatgcgtc gcccgcaccg gctgcgcgtc
     2221 atcccgaact tcgtcgatgt cgagctcgtc caaccgggac ccaccgagaa ctcctatcgc
     2281 gaggagtacg gattgtcggg caagaccgtg gtgatgtacg ccggcaatgt cggcatgtcg
     2341 cagtcgctcg accttctcgt gcacgccgcg caatcgactc gtgaccgcga tgacatcgtg
     2401 tacgtcatca acggcggcgg gtcgacgctg cccgatctgc aggcgcaggc caccgacctg
     2461 ccgaacctgc ggttcatacc gatgcagccc ctggatcggg tgcccgagct actcgcagcg
     2521 gcggatcttc acgtcgtccc gttgaaggct gggttggcac ggtcgagcgt gccatcgaag
     2581 acctactcca tcatggccgc gggtcgacct ctggttgcca gcgtcgacgg gggaagcgaa
     2641 gtgcagcgcg tcgtcgaggc tgcgggcgcg ggcatcgcgg tgccccccga cgatgccgac
     2701 gcgttcgcgg ccgcggtgtt gaagctcgcc gacgacgcgg gcgccagaga gaagatgggc
     2761 gcggcggccc gcgcgtatgt cgagcggacg gcgtcaccgc gcggcgtcgc gcaggcctac
     2821 gccgatctct tcgagtcagt cgtgcgccac aagtagaggg tcgcggcgag gaggccgaag
     2881 cctcgctcga tcagccgcga aatgcgcgcg gcggcgcggg catgtcgatc cgcgggcagt
     2941 tgctcagggt cagatcggcg tatcggagcg cctcgttgca cagcgagtca acggtgtagc
     3001 cggtacgcga cccgacgacg gtgatcgttg cccggaagat ggtgatctcc cagcgggtgg
     3061 gcatgccgta ggcaacctga atactgccga tgaggccagc cgccgcggcg gagcgctcgt
     3121 ccttgccctt ccacgggcgt tcgaacagcc gccagcagcc cggctcggcg tgccagcggc
     3181 cgccagagcg gacgtcctcg gcccgtagcc cgtcaagaac ccgacgggcg ttcctctcgg
     3241 agaggaccgc tgccggtcgg atgacctcgg tcacggtgac gtcgtcgtag agccggctgg
     3301 cgagactctc aaccgcctca cgctgggcgg cgcccggcac tttgtccttg ctcacggctc
     3361 ttgtcacccg atttcctgga catccgtgca gtggttagca gtggcggtca gtagccatcg
     3421 tgaatcagcc gtgcaggcgg ctacagtgct tatcggccac ccaaggggcc cgctg
//
LOCUS       DATCBJ010000468         3001 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_307295, whole genome shotgun sequence.
ACCESSION   DATCBJ010000468 DATCBJ010000000
VERSION     DATCBJ010000468.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 3001)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3001)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3001
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_307295"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..236)
                     /locus_tag="VII50_09460"
     CDS             complement(<1..236)
                     /locus_tag="VII50_09460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5051118.1"
                     /translation="MLAFTGPGALLLTGAEALGLTVATPGDETAAVVGAPNGLAADGA
                     FLPAAASQVTPPASTTTRATAITSDRRGRGHRAGS"
     gene            241..1284
                     /locus_tag="VII50_09465"
     CDS             241..1284
                     /locus_tag="VII50_09465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5051119.1"
                     /translation="MPTLSSPAAPPALVGPAPTNTGVRLLVAFSAPTGGVGIFALDGG
                     AISAVRGFPAGGCPEGSVFRIRATEQWAAVWQPTSASQPCGLAANRLYIIDAATSTAH
                     LIGSAEGVVTADRSSLWTVAGIDLRPAQQLLVPEHVQRISLTGAILSRVYALPVGWTV
                     IKGLTPDLLLLARDLANGLDNWEAWQPSTGAVLGQYERVLDANANVVVWVNNTCAPDN
                     CPIHLSPPAGGADRVVSLPAGGYAYDGSLSDDGKYLALSLGTGVDSEGATDQDTGVLV
                     DIATRAVHPIQQTKIPASETGSLSLNWGPHGWLIVSTPGPNRTSQLAAYNPTTGTFVV
                     SPHRTPADEWVAH"
     gene            complement(1430..1822)
                     /locus_tag="VII50_09470"
     CDS             complement(1430..1822)
                     /locus_tag="VII50_09470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5051120.1"
                     /translation="MLRKTIATVSPSAPAMAFDGLVNCRLPALQISITGPVEELAGAS
                     PVGRLDVLAVSVGGTVPVADVVFWLGDATPAVGVAEPYPGAELEELRSSSAPLLLLVH
                     PPITVVARRQATAVNLAGTGLGWGGTTE"
     gene            1722..2228
                     /locus_tag="VII50_09475"
     CDS             1722..2228
                     /locus_tag="VII50_09475"
                     /inference="COORDINATES: protein motif:HMM:NF025382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4232 domain-containing protein"
                     /protein_id="HEY5051121.1"
                     /translation="MIDICKAGSLQFTNPSNAIAGAEGETVAIVFRNMGPQPCSLTGW
                     PTISTPGLHTKVQYSTFTGAGFEVAVSRLVLQPGETGATALDIFGSPGSDYGTQCFAA
                     GWWAVTLPGSHQATSVPWPKYQGACPGGTIYVSPVYSGNQPEIGFGSADPTSIPPLGP
                     FDSPPASP"
     gene            complement(2491..>3001)
                     /locus_tag="VII50_09480"
     CDS             complement(2491..>3001)
                     /locus_tag="VII50_09480"
                     /inference="COORDINATES: protein motif:HMM:NF024928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding domain-containing protein"
                     /protein_id="HEY5051122.1"
                     /translation="YRVLSPTIDGGLLVDDLGGRGRTLLPAAYVAEHVDYGWATTIDA
                     AQGSTADVAVLLVRPGLDREHLYVAMTRGRAENHAYIANNHDNECDQQRTNPPPDAER
                     ILIDALARSGQQHAAHTLLEKAKNATPASRRDEPTAVPTPSAGRWRPAGPRTRDVPGP
                     GTTSSVRTL"
BASE COUNT          504 a          999 c          977 g          521 t
ORIGIN      
        1 gacccggcgc ggtggcctcg gccacggcga tcgctggtga tcgcggtggc cctggtggtt
       61 gtgctggccg gcggcgtgac ctggctcgct gcggcaggaa gaaaagcgcc gtcggcggcc
      121 aagccgttcg gtgcgccgac gactgcggcg gtttcgtcac cgggcgtcgc gacggtgagc
      181 ccgagtgcct cggcgccggt gagcagcagc gcgcctgggc cggtaaacgc gagcacgttc
      241 gtgccgaccc tgtcatcgcc ggcggcgccc cccgcgttgg tggggccggc tccgacaaac
      301 actggtgtcc ggctgctcgt cgcgttctcc gcaccgactg ggggtgtcgg gatctttgcc
      361 ctcgacggtg gtgcgatatc agcggtgcgc ggcttccccg cgggtggctg cccggaaggc
      421 agtgtcttcc ggatacgggc caccgaacaa tgggcggcgg tctggcaacc aaccagcgcc
      481 tcgcagccgt gcggattagc cgccaaccgc ctttacatca tcgacgccgc cacttcgacc
      541 gcgcacttga ttggctccgc ggagggcgtc gtcaccgcgg atcgcagctc gctgtggacc
      601 gtcgccggta tcgatctgcg acccgcccag cagctcttag tgcccgaaca tgtgcaacgg
      661 atcagcctga ccggtgcgat tctcagccgc gtctacgccc tgcctgtggg atggacggtg
      721 atcaaaggcc tcacccctga cctgctgctg ctcgcccgcg acctcgccaa cgggctcgac
      781 aactgggagg cttggcagcc cagcaccgga gctgtactcg gtcaatacga gcgcgtgctc
      841 gacgcgaacg ccaacgtcgt ggtctgggtg aacaacacct gcgcgccgga caactgcccc
      901 attcatctca gccccccagc tggtggcgct gaccgcgtag tgtccctgcc cgccggcggc
      961 tacgcctacg acggatcgct tagcgacgac ggcaaatacc ttgcgctgtc gctaggtacc
     1021 ggagtggaca gcgaaggggc caccgaccaa gacaccggcg tcctggtcga catcgccacc
     1081 cgagccgtcc acccaatcca gcagaccaaa atccctgcaa gtgagacggg cagcctgtca
     1141 ctgaactggg gaccgcacgg atggctgatc gtgagcaccc ccgggcccaa ccggaccagc
     1201 caactcgccg catacaaccc caccactggg accttcgtgg tctcaccaca caggacgccg
     1261 gccgacgaat gggtcgccca ctagcgtcct ttccgcactg ccgcccgctt gcacgaagac
     1321 gatctaccga gttcatcgat cgtcgaccca acgcgatggg acaaactcgc gtacggccgc
     1381 aaatgggggc ggttccgtgg gatgcactat ccggtgcgtt tccgtggaac tattcggttg
     1441 tgccgcccca tccaaggcct gtgccagcaa gattgacagc cgttgcctgc ctccttgcca
     1501 cgaccgtgat tggcgggtgc acgagcagca gaagtggtgc gctcgatgac cggagttctt
     1561 caagctccgc gcctgggtac ggctcggcaa caccgaccgc cggagtcgcg tcgccgagcc
     1621 agaacacgac atcggcgacc gggactgttc ctcccaccga cacagccagt acgtcaagcc
     1681 ttccgaccgg cgacgcacca gcaagctcct cgaccgggcc tgtgattgat atctgcaaag
     1741 ccggaagtct gcagttcacc aacccgtcga acgccatcgc tggcgcggaa ggtgaaactg
     1801 tcgcgatcgt cttccgcaac atggggccgc agccttgctc gctcacaggc tggcccacga
     1861 tcagcactcc ggggctccac accaaggtgc agtattcaac cttcacaggt gcaggattcg
     1921 aagtcgcagt cagccgcctc gtgctgcaac caggtgaaac cggcgccaca gcgctggaca
     1981 tctttggcag ccccggcagc gactacggca cacaatgttt cgccgctggt tggtgggcgg
     2041 tgactcttcc cggcagccac caagcgacat ccgtgccatg gccgaagtac cagggtgcct
     2101 gcccaggcgg caccatctat gtatcgcccg tctacagcgg caaccagccg gaaatcggct
     2161 tcggttcagc cgaccccacg agcattccgc cgctcggacc gttcgacagc ccgcctgcct
     2221 cgccgtgagc gggctatcga actgccacgg tgctgactcg cggggagttc gctgatcggc
     2281 cccagcgtaa ccccttcatc tgcttgccgc aggtcacacc gacacgatca gtctgtataa
     2341 ccaccatttg caaatctgat agccagcgca gttgatcgga atgcactgat tcgaggagtg
     2401 aaaacctgaa cgaggtcacg gaatgcaccc aaccccaacg tccgcacatc ggcaaatgat
     2461 tgcgtttgcg aagcgatttc gtgcgaccgg ctagagcgtt ctgacggagc tggtcgtgcc
     2521 tgggccgggt acgtcgcggg tgcgcggccc cgcggggcgc cagcggcccg ctgacggtgt
     2581 ggggacggcg gtgggttcgt cgcgtctgct ggccggggtc gcgtttttgg ctttttcgag
     2641 cagggtgtgc gcggcgtgtt gttggccgct gcgggcgagc gcgtcgatga gaatccgctc
     2701 cgcgtcgggt ggtggattgg tgcgctgctg gtcgcactcg ttgtcatggt tgttcgcgat
     2761 gtaggcatgg ttttctgcgc gtccgcgggt catggcgacg tagaggtgtt cgcggtcgag
     2821 gcctgggcga actagcagca cggcgacgtc ggcggtggat ccttgagcgg cgtcgatggt
     2881 ggtggcccag ccgtagtcga cgtgttcggc gacgtatgcg gccgggagga gcgtgcggcc
     2941 gcgaccgccc aggtcgtcga cgagcagtcc gccgtcgatg gtgggggaca gcacccggta
     3001 g
//
LOCUS       DATCBJ010000469         4225 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_194911, whole genome shotgun sequence.
ACCESSION   DATCBJ010000469 DATCBJ010000000
VERSION     DATCBJ010000469.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 4225)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4225)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4225
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_194911"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(17..2071)
                     /locus_tag="VII50_09485"
     CDS             complement(17..2071)
                     /locus_tag="VII50_09485"
                     /inference="COORDINATES: protein motif:HMM:NF013884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HEY5051123.1"
                     /translation="MIKITGAGVDVTTAKSIAVTRTAGPAPATARSGGAARRLPGLDG
                     LRAIAVIGVLLYHAGVGFLPGGFLGVDLFFVISGFLITSLLLSEARLAGRLSLRQFYL
                     RRARRLLPALVVMLAVVAVFMAIFASADLGQARGDIAAALGYVSNWWYVLHHRSYFIA
                     AGRPSPFAHLWSLAVEEQFYLIWPAVLVVLVATRARLRWITAVALAGAIGSALWMYIL
                     AVRGNVPFDTDSSRLYFGTDTHASALLLGAAAAAVMAGLAGRLDRGIAVGVRAAWDIV
                     GVAALLAVCWSMHAFDYYRPGLYRGGFLAFAAAGVVVVVTASAPAGRLGWALDVPPMR
                     WIGARSYGLYLWHWPVFVYTRPGLDWSLHGPAALAVRLAIVAVLTEMSYRFVEVPLRR
                     DGIVSLARKLRVPRTWRVMAPVLSAVLGVAGVAGATAFVSSRVAHTQARAGRTAATLD
                     EGQSSPSHGTVAPTRSTSPAPAASSATSTVAPSSTAAIPAPSATTLPVVSWPTHPPSR
                     PIHPGTGPHVAATEPPAIPVGTPPSVSAVGDSVMLDAATALKGVCPGTEVYAVVGWQA
                     KSVFAELTALRTAAHLGREVVIETGTNGIVSSKELDATLTALADRQKVVVINNHMDRP
                     WEPPNNAMFPVVVKAHPNAVLVNWDAAANTHPNWLSADGVHLKPAGRAPYAQLIKVAA
                     AC"
     gene            complement(2269..>4225)
                     /locus_tag="VII50_09490"
     CDS             complement(2269..>4225)
                     /locus_tag="VII50_09490"
                     /inference="COORDINATES: protein motif:HMM:NF024905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HEY5051124.1"
                     /translation="SVAPTPRPRIAVLTSTAAATALGTFVGSATSGYAGLHVVAVAFF
                     TFVGGLLVAVEPAVTAVGLNAIVGLVVYGRFPGSPETALKSAGLVAAGGLFQVLLVVV
                     LRGPPQVGRALAGLSRAYVALATYAADLAIDRSGLSMAAAIDAAVVDREFSFGAGMSG
                     SPGEACTSLVDEARRIRLELLSLASARAAAQGADEPDDALLASFEQLASRLRDFFDHV
                     VDGLAHADVPADLDDALAAATDAIEDIRRVAAAAPHARLIAARATTAGAALAGQLRAV
                     AGLLPAAVEARRTVRTSAVRSATRISRRGAQGAEVIADRMRANLTWQSDAFQHAVRLA
                     AVVSAAAVIAHVVGIGRGYWLALTAVLVLRPEFSVTFTRGVGRAVGTFLGVGLATIVA
                     VTANPHGWVLVPFVGVFVWLAGAVFNASYAAFSVAVTGAVVFLLAGLDTDPVLDARDR
                     LVATVLGAVLALGAYALWPSWGRRPVADAIADLADTTHRYVGIVLRAVVDPAADISAA
                     SAASRAVRLARTNAEAAINRSLGDPASRRIDQTLTLETLAAFRRLAIAAHTLRLSPRL
                     ILDGVPPALNELTAAIDGELEAIAARLRFGRVTVVRQPLRALHREVAAALPGGAEAPG
                     TLVVAETDEIVDTTNSLADIVERMPPA"
BASE COUNT          747 a         1445 c         1478 g          555 t
ORIGIN      
        1 gatcgccgcc ggcatctcag cacgccgcgg ctaccttgat caactgcgcg tagggtgcgc
       61 ggccagccgg cttcaggtgc acgccgtcgg cgctcagcca gtttgggtgc gtgttcgctg
      121 cagcatccca gttgaccaag acggcattgg ggtgcgcctt caccacgacc gggaacatgg
      181 cgttgttggg cggctcccat ggccggtcca tatggttatt gatgacgacc accttctgcc
      241 ggtccgcaag cgcggtgagg gtggcgtcca actccttcga actgacgatg ccgttggtgc
      301 cggtttcgat caccacctcg cggcccaggt gtgcggcagt acgcagcgct gtcagttcgg
      361 cgaacaccga cttcgcctgc cagccgacga cggcatacac ctcggtaccg ggacacacgc
      421 ccttcagtgc tgtcgcggcg tcgagcatga ccgagtcgcc gacggcgctg accgacggtg
      481 gggtcccgac cgggatcgcc ggcggttcgg ttgccgcgac atgtgggccg gtgcccgggt
      541 ggatcggacg tgatggcggg tgcgtgggcc acgacacgac cggcaatgtc gtcgccgacg
      601 gcgccggtat cgcggctgtc gagctcggcg caaccgtcga cgtcgcggac gacgctgccg
      661 gtgcgggcga cgtcgatctc gtcggcgcga ccgttccgtg gctcggcgac gactgcccct
      721 catcgagggt cgcggcggtg cggccagccc gggcttgcgt gtgggctacg cggctcgaca
      781 cgaacgcagt cgcccctgcc acgccggcga cgccgagcac ggcagacagc actggcgcca
      841 tgacccgcca cgtgcgcggc acccgaagct tgcgggccaa cgacacgatg ccgtcgcgcc
      901 ggagcggaac ctcgacgaac cggtaggaca tttcggtcag cacggcgacg atggccagcc
      961 ggacggcgag ggcagctggg ccgtgcaacg accagtcgag accgggccgg gtgtagacga
     1021 aaaccggcca gtgccatagg tagaggccat acgaccgcgc gccgatccat cgcatcggcg
     1081 gcacgtcgag tgcccacccg agccggcccg caggggccga cgccgtgaca acgaccacca
     1141 cgccggcggc ggcgaaggcg aggaagccgc cgcggtacag acccggtcgg tagtagtcga
     1201 acgcgtgcat cgaccagcac acggcgagca gcgcggcgac accgacgatg tcccatgcgg
     1261 cgcggacgcc gaccgcgatc cctcgatcga gtcggcctgc caggccagcc atcactgctg
     1321 ccgccgcggc accgagcagc agcgcgctcg cgtgcgtgtc ggtgccgaag tacaaccgcg
     1381 acgagtcggt atcgaacggc acgttgccgc gcaccgcgag tatgtacatc cacaacgccg
     1441 agccgatcgc accggccagc gcgacggcgg tgatccatcg caggcgggcg cgggtcgcca
     1501 ccaacacgac cagcacggcc ggccagatca ggtagaactg ttcctcgacg gcgagtgacc
     1561 acaaatgggc aaacggcgac ggccggcccg ctgcgatgaa gtacgaccgg tggtgcagga
     1621 cgtaccacca gttcgagaca tagcccaagg cggcggcgat gtcaccacgc gcctgtccga
     1681 ggtcggcgga cgcgaagatc gccatgaaaa cagccactac ggcgagcatg acgacgagtg
     1741 ctggcagcag tcgccgggcg cggcgcaggt agaactgacg caacgagagg cgacctgcca
     1801 gccgcgcttc gctcagcagc agcgacgtga tcaagaagcc ggagatgacg aagaacaggt
     1861 cgacgccgag gaatccgccg gggaggaagc ccacgcccgc gtgatacaga agcacgccga
     1921 tcacggcgat cgctcgtagc ccgtcgaggc caggcagtcg gcgagcagca cctccggaac
     1981 gcgcggtggc gggtgcaggc ccggctgtcc gggtgaccgc aatggatttc gccgtcgtca
     2041 cgtcgacccc tgcccccgtg atcttgatca tcgaaccgtt ctcatggagc gtcgcaagcg
     2101 agccgagtgt gacattgctt cgtctcgccg ccggttgttc ttgaccggcg cgccgcaacc
     2161 gttgccagat cgagtccggg cacgggtttt gcaggtcatg gcgaatcgac gcgcgcgggc
     2221 gctgattggt tgcgtcggcg tccgcgctac ttcatcggga cggcgccgtc aggctggggg
     2281 catgcgctcg acgatgtcgg cgagtgagtt cgtcgtgtcg acgatctcgt cggtctcggc
     2341 gacgacgagg gttcccgggg cttcagcgcc acccggcaag gcggccgcga cctcccggtg
     2401 cagggcgcgc agcggttgcc gcaccacagt gacccgcccg aatcgcagtc gtgccgcgat
     2461 cgcttcgagc tctccgtcga tggcagcggt gagttcgttc aacgcgggcg ggacgccgtc
     2521 caatatcagc ctcggcgaca accgcaaggt gtgcgcggcg atggcaagcc gccggaaagc
     2581 ggccagcgtc tccagggtga gcgtctggtc gatgcgccga gaggccgggt cgccgagcga
     2641 gcggttgatc gcagcctcgg cgttggtgcg cgctagccga accgcgcgac tcgccgccga
     2701 agccgcggag atatccgctg ccgggtcgac gaccgcgcgc aagacgatgc cgacataccg
     2761 gtgggtggta tcggcgagat ctgcaatcgc gtcggcaaca gggcgacgtc cccagctggg
     2821 ccacagcgcg tacgcgccaa gtgcaagcac agcgccgagc accgtggcga ccaggcggtc
     2881 gcgcgcgtca agcaccggat cggtatcgag gccggcaagc aggaagacca cagcgccagt
     2941 gacggccaca ctgaacgcgg catagctcgc gttgaacacg gcgcctgcca gccacacgaa
     3001 cacaccgacg aacgggacaa gcacccatcc gtgcggattc gcggtgacgg cgacgatcgt
     3061 ggcgaggcca acgccgagaa acgtgccaac cgcccgcccg acgccgcgag tgaaggtcac
     3121 ggagaactcg ggccgcagga cgaggaccgc ggtcagggcc agccagtagc cgcgtccgat
     3181 cccgaccacg tgggcgatca cggctgccgc cgagacgaca gctgcaaggc gcaccgcgtg
     3241 ctggaacgca tccgactgcc aggtgaggtt cgcccgcatc cggtcggcga tgacttcagc
     3301 gccctgcgca ccgcgacggg agatgcgggt cgccgatcgg acggcagatg ttcgaacagt
     3361 gcgcctggcc tcgaccgcgg caggcagcag gccggcgact gcccgcagct ggccggcgag
     3421 tgccgctccc gcggtcgtgg cgcgtgctgc aatgagccgg gcgtgcggcg cggcggctgc
     3481 gactcgcctg atgtcttcga tcgcgtcggt ggccgcggcg agtgcgtcgt ccagatcggc
     3541 gggaacgtcc gcatgcgcca acccgtcgac gacgtggtca aagaaatcgc gcaggcgaga
     3601 cgcgagctgc tcgaaacttg ccagcaacgc gtcgtctggc tcatcggcgc cttgcgcggc
     3661 tgcgcgggcg ctcgccaggc tcaagagctc gagtctgatc cgccgcgctt cgtcgaccag
     3721 gctcgtgcac gcctcaccgg gcgagccgct catacctgca ccgaaggaga attcacggtc
     3781 gacgaccgcc gcatcgatgg ccgcggccat tgacaatccc gaccgatcga tggcgaggtc
     3841 ggccgcgtac gtcgcgagag cgacgtatgc gcgcgacaac ccggcgagcg cccggccgac
     3901 ctggggggga ccgcggagaa cgaccaccag cagaacttgg aacaggccac ccgcagcaac
     3961 caggccggca ctcttcaatg cggtttcggg cgaccctgga aaccggccgt acacgacgag
     4021 cccgacgatc gcgttcagtc cgaccgccgt gacggccggc tcgaccgcaa cgagcaggcc
     4081 gccaacgaac gtgaagaacg cgacggccac gacgtgcagg cccgcgtatc cactcgtcgc
     4141 cgacccgacg aacgtcccga gcgccgtcgc cgccgcggtc gaggtcagta cggcaatccg
     4201 cggccgaggc gtcggtgcca ctgac
//
LOCUS       DATCBJ010000470         2239 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_15518, whole genome shotgun sequence.
ACCESSION   DATCBJ010000470 DATCBJ010000000
VERSION     DATCBJ010000470.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2239)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2239)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2239
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_15518"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..766
                     /locus_tag="VII50_09495"
     CDS             <1..766
                     /locus_tag="VII50_09495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HEY5051125.1"
                     /translation="PPTRGAFYRAAIRSAVKRSTRCIVPSKATRDELVRLVDADSTRI
                     DVAYHGVDHDVFRPPTEAEAARISARLGLRGQQYIAFLGAFEPRKNVPSLIRGWVSAV
                     GDSAESPALVIAGGAGWDDEIEAAVAEVPSHLRVIRPGYLRMADLSAFLGGALVVAYP
                     SHGEGFGLPVLEAMACGAAVLTTHRLSLPEVGGDAVAYTEPDSASIGTALSGLLADEP
                     RRRALGGAAHARSLEFTWEASAQAHLQAYTRAVSGD"
     gene            858..2000
                     /locus_tag="VII50_09500"
     CDS             858..2000
                     /locus_tag="VII50_09500"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEY5051126.1"
                     /translation="MRVLHLTSSFPRDARDHVAPFLLDLAHAAQEAGMDVGVLAPHDK
                     GLATVDTVAGVEVHRFRYGLQSMERLSYRGGLLGSSRRFSGVMLPAYLASFSRAAEKA
                     ARDFRPDVIHAHWWLPAGLCALPVARRLDVPLIITVHGSDVHLLRYRALRQLASHVMH
                     RASLVATVSEDLQRQILEFDPRLHTSVLRLPVPAGPPAVPMPADPPIRLLAAGRLSHE
                     KGFDVLIEAMRIAVGSGLDVRLDIVGSGPERRRLALLAAPLGDRIQLIPALPHAELMH
                     RLEGVHALVAPSRREGLGMVALEAFTRGRPVVASRVGGLAESVEDGVDGYLVEPEDPI
                     ALAAALARLPFGPPRGVATLRHSSIEVGRSHLEAYASLVSPMVKSR"
BASE COUNT          324 a          764 c          755 g          396 t
ORIGIN      
        1 cccgcccacg cgcggcgcgt tctaccgtgc ggcgattcgc tcggcagtga agcgctcaac
       61 gaggtgcatc gtgccgtcga aggccacccg cgacgagctc gtccgcctcg tcgacgccga
      121 ctcgacccgt atcgacgtcg cgtaccacgg cgtcgaccac gacgtgttca ggccgccgac
      181 agaggcggaa gccgcgcgta tctccgcgcg acttggccta cgcggccagc agtacatcgc
      241 cttcctcggc gctttcgagc cgcgcaagaa cgtgccgagc ctgatccgag gctgggtcag
      301 tgccgtcggc gactcggccg agtcgcccgc gttggtcatc gccggcggag caggttggga
      361 cgacgagatc gaagctgcgg tcgccgaggt gccttcgcac ctgcgggtga tccggcccgg
      421 ctacctgcgg atggccgacc tctcggcatt ccttggtggc gcgcttgtgg tcgcctatcc
      481 cagccacggc gagggtttcg ggttgccggt gctcgaagcg atggcgtgtg gtgccgccgt
      541 gctgacgacg caccggttgt cgttgcctga ggtcggtggc gatgcggtcg cctacacgga
      601 gcccgactcc gcttcaatcg ggacggcgct gtcggggttg ctcgccgatg agccacgccg
      661 tcgcgcgctc ggtggcgcgg cacacgcgcg atcgctcgag ttcacttggg aagcaagcgc
      721 tcaagcgcat ttgcaggcct acaccagggc tgtgtccggc gactgaccgc tcacgcagcg
      781 gcgcggaacc ttaacccagc gccgacccac acgccagtac tcggacattc cgtgctctga
      841 ccgttacgct acgagacgtg cgcgtgcttc atttgacctc ctcgttcccg cgcgatgcgc
      901 gggaccacgt cgcgccattt cttcttgacc ttgcgcacgc agcacaagaa gcgggcatgg
      961 acgtcggggt cctcgcgccg cacgacaagg gcctggcaac cgtcgacacc gttgccggcg
     1021 tcgaggtgca ccgcttccgc tatggcctgc aatctatgga gaggctgtcg tatcgcggcg
     1081 gcctgctcgg tagctcgcgg cggttcagcg gcgtcatgtt gccggcatac cttgcgtcgt
     1141 tctcgagggc ggccgagaag gcggcccgcg acttccgacc ggacgtcatc cacgcgcact
     1201 ggtggttgcc ggccggattg tgcgcattgc cagtagcccg gcgcctcgac gtcccgctca
     1261 tcatcactgt gcacggctcc gatgttcacc tgctgcgcta ccgcgcgctg cgccaactcg
     1321 cttcgcacgt catgcaccgg gcatcgctgg tggcgaccgt gtccgaggac ctgcagcggc
     1381 agattctcga gttcgatccg cgcctgcaca cgtcggtgct gcggctgccg gtgcctgctg
     1441 ggccgccggc ggtgccgatg ccggccgatc cgccgatccg gctgctcgcg gccgggcggc
     1501 tgtcgcacga gaagggcttc gacgtcctca tcgaggccat gcggatcgcg gtgggcagcg
     1561 ggctcgacgt ccggctcgac atcgtcggct cgggccccga acgacgccgt ctcgcgctgc
     1621 tggccgctcc gctcggcgat cggatccagc tcatccccgc cctgccgcac gccgagctga
     1681 tgcacaggct tgagggagtt cacgcgctgg tggcgccgtc gcgccgcgaa ggcctcggca
     1741 tggtcgccct ggaggcgttc acccgtggcc ggccggtggt cgccagccgg gtcggcggcc
     1801 tggccgagag cgtcgaggac ggcgtcgacg gttacttggt cgagcctgag gacccgatcg
     1861 cgctcgccgc agcgctggca aggctcccgt tcgggccgcc acgcggagtg gcaacgttaa
     1921 ggcactcttc aattgaggtt ggcagaagcc acctcgaggc ttatgcctcc cttgtctcgc
     1981 ccatggtcaa gagccggtaa gttcagggaa ctagatcgga cgaatgtccg gtttggaaac
     2041 caatccacga cacgacatac gtcgtgggtg gggcgtctgg cttccggcag cgtgaaacct
     2101 gtaaggtccc aaatcgagcg tttgctcggg tatcggcggg gctttcagga ttgcgccttc
     2161 gggctgagcc acctcgccac caccacttct ctgatggagg caagagtgac aacagcaacc
     2221 agacggttgc gtcgagcca
//
LOCUS       DATCBJ010000471         7795 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_120788, whole genome shotgun sequence.
ACCESSION   DATCBJ010000471 DATCBJ010000000
VERSION     DATCBJ010000471.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 7795)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7795)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7795
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_120788"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..524)
                     /locus_tag="VII50_09505"
     CDS             complement(<1..524)
                     /locus_tag="VII50_09505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5051127.1"
                     /translation="MGVRVAALVGSPELLVNAMFDPMGATRAEANILRAVVGPRGLTA
                     LAASLEVDSMLLKAVIAKEQYADDLPVPQVAALERWLVTLPPELAINPTRTLRDGTRD
                     SAAHANAITGYAAPHTQALLELFAPSLRFRLDLALRRPLPVDPVFGVPLAPLVPDGEE
                     RAAVFRYRDICARGP"
     gene            complement(722..937)
                     /locus_tag="VII50_09510"
     CDS             complement(722..937)
                     /locus_tag="VII50_09510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5051128.1"
                     /translation="MSNIDEIELELDQRAVQVEMAGAQLVRAAAEAIWTSTAADAFRA
                     RVDHRRRQFGAVADLLRGAAQTVFRYS"
     gene            1100..1603
                     /locus_tag="VII50_09515"
     CDS             1100..1603
                     /locus_tag="VII50_09515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5051129.1"
                     /translation="MPWLPADFVHPLRAEVDAEHHLRPIRATDVDLDMVAVMSSQARL
                     WSIFGPAWQWPPPSMTKEQDRADLARHELEIEAYQSFNYALFDSAQTALLGCVYIDPP
                     EKHGADAEISWWVIDQLLDSETETSLNELVPRWIATHWPFTNPRYIGRDIMWTDWLDL
                     PDADQPS"
     gene            complement(1604..2179)
                     /locus_tag="VII50_09520"
     CDS             complement(1604..2179)
                     /locus_tag="VII50_09520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009072508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HhH-GPD-type base excision DNA repair protein"
                     /protein_id="HEY5051130.1"
                     /translation="MTLHIAQQPDADALLDRDPLALLVGMLLDQQFPMERAFAGPYLL
                     AQRLGVDHLDAYDMAEHDPVTFATLFVGPPAIHRFPGAMAGRVQALARLLVADYEGRA
                     EAVWDDAKSGAELHARLATLPGFGAQKARIFVALLGKQRGVRPRGWREAAGPYGEDDS
                     YRSVADVTSSESLQKVREHKKEMKAAARAGA"
     gene            complement(2242..2850)
                     /locus_tag="VII50_09525"
     CDS             complement(2242..2850)
                     /locus_tag="VII50_09525"
                     /inference="COORDINATES: protein motif:HMM:NF019533.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1684 domain-containing protein"
                     /protein_id="HEY5051131.1"
                     /translation="MTLLDWRRRVHAVYTAVRAATDRPAAHASWSAERDNLFAHHPQS
                     PIAVDRRAAFTGVPVKAYDPAWRFELPMDTDIEPSHIEIATGTDGIVPFDRTGVVRIP
                     DVGQLDVWALRSYGGGLFIPLRDRTANRTTYGGGRYLIDTAKGADLGSDAAAGTIVLD
                     FNFAYHPSCAYDAAWACPLAPPGNVLDVDVPVGEQLPTGGWY"
     gene            complement(2882..4873)
                     /locus_tag="VII50_09530"
     CDS             complement(2882..4873)
                     /locus_tag="VII50_09530"
                     /inference="COORDINATES: protein motif:HMM:NF012961.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 9 protein"
                     /protein_id="HEY5051132.1"
                     /translation="MGRTVFRRPSTWISLPAATVLFAGVALGSLAAAPADAVASQSAP
                     AALVRVDQVGYLPTDAKHAYLMANQRVPHATWDVVDAANHVVAHGHVGTTNRGPWNTN
                     YPDVYDITFSGLGALGTYHLAVHGDATAQSPSFAIESAASLYSQVVLDGVNFYQVQRD
                     GADVVPGPLDRQPAHLGDAHATVYQTPDFVDTTSGDSLVPDSPLIKIKGAPTVNAEGG
                     WYDAGDFLKFTHTAAYGDVVLYASERALGNSAPSTLKTEAAYGENWLDKMWQMSTKTL
                     YLQVGVGTGNGSDTVDPTFNGDHDLWRLPQADAADTDPMDRYATVDRPVFEAAAPGHQ
                     ISPNLVGRVSAAFALAAQVDAVTNPARAQYEYLEATSLYAMANTSHPPSPLVTADPEA
                     YYPETTWHDDMELGGAEIALAAQKLSHNAMPYLVAAAGFAKNYIADDAAAGDTFNLYD
                     TSALAHTDLVHAMAAAGNPTLAVSKAALIKDLKRQVELGATHAAGDTFRAAGDINQFD
                     VDSHTFGWIAMEAWYAELTGDHRFDAFAAEQRDWLFGANAWGTSFMVGEGSTFPDCMQ
                     HQVANLSGNLHGVGPALDVGAVVNGPNDNANFEGGLGGLQDGMAKCPTTGVDKFAAFD
                     TSTASFVDDVRSWQTDEPALDMTGAAIIAAASQLALHKQ"
     gene            5094..5414
                     /locus_tag="VII50_09535"
     CDS             5094..5414
                     /locus_tag="VII50_09535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5051133.1"
                     /translation="MKLGAYNWLVLATSRATQLLAVFLAVVSSLPDAEAALDAGVEGA
                     ALALEAQVAAAVCGGRALTSVAIPRTRYRTKNWLRSRPATGRPSSCPPPIGPRARLQF
                     RWVL"
     gene            complement(5971..6537)
                     /locus_tag="VII50_09540"
     CDS             complement(5971..6537)
                     /locus_tag="VII50_09540"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009079466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase family protein"
                     /protein_id="HEY5051134.1"
                     /translation="MSTLENRPNTALIVIDVQNGIVGEAYDRDAVVANIATLVDKARA
                     ADVDVVWVQHNSDDLPKDSEGWQYVPELVRRESEVLVQKAYADSFEETDLESVLAARG
                     IGQLIISGAQTDECIRSTLHGAIVRGYDATLVSDAHTTEDQSQWGAPPPDKVIAHTNL
                     YWKYHTAPGRSAGTVTTADLDFAAKPST"
     gene            6640..7254
                     /locus_tag="VII50_09545"
     CDS             6640..7254
                     /locus_tag="VII50_09545"
                     /inference="COORDINATES: protein motif:HMM:NF016840.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEY5051135.1"
                     /translation="MSESDPKADLRRYLQVAREAVLWKLDALSEYEIRRPMTPAGTNL
                     LGLVKHLANVEVGYFGETFGRPFDGPLPWVEGPEGEEPDPNADLWATVDESRDQIVGL
                     YQAAWAHADKTIETLDLATIGAVPWWPADHAEVTLHKVLVHVIAETNRHAGHADIVRE
                     LIDGATGLRAGNHNMADGDQAWWQEYRNRLEQAARDASPEQLHD"
     gene            complement(7271..7492)
                     /locus_tag="VII50_09550"
     CDS             complement(7271..7492)
                     /locus_tag="VII50_09550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5051136.1"
                     /translation="MVAGDEAASDDAGADAGLVDGPVTPEERCLEATLSQALSCSAAA
                     THTPVNVAATAFRLTTMTNRRSNASLGCI"
     gene            7491..>7795
                     /locus_tag="VII50_09555"
     CDS             7491..>7795
                     /locus_tag="VII50_09555"
                     /inference="COORDINATES: protein motif:HMM:NF026220.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YCF48-related protein"
                     /protein_id="HEY5051137.1"
                     /translation="MPASQPATSSGPPSACALPTPDGGWGTWLRAAQFVSPTKGWVVG
                     TAGILATTDGAHWSWQYGAATAAFDGVDFIDSEHGWAVGPNTLVRTTDGGTHWQQLT"
BASE COUNT         1306 a         2598 c         2589 g         1302 t
ORIGIN      
        1 ggcccacgcg cgcatatatc tcgatatcga aacactgccg cccgttcttc gccatccgga
       61 accagcggcg cgagcggcac accgaagacg ggatcgacgg gcagcggccg acgtagagcg
      121 agatcgagac gaaaccggag cgacggcgcg aacaactcga gcagcgcctg cgtgtgcgga
      181 gctgcgtagc cggtgatcgc gttggcatgc gccgcgctat cgcgagtgcc gtcgcgcagt
      241 gtgcgagtgg gattgattgc caactccggc ggcaacgtga ccagccatcg ctcgagcgcg
      301 gcgacttggg gcactggaag gtcgtccgcg tactgttcct tggcgatgac cgccttaagg
      361 agcatcgagt cgacctcgag gcttgcagcg agcgcggtca ggccacgcgg gccgaccacg
      421 gcgcgcagaa tgttcgcctc cgctcgagtc gcacccatcg ggtcgaacat cgcgttcacc
      481 aggagctcgg gcgagccgac cagcgccgcc acacggacac ccactcgtgt catcgatgcg
      541 gctccacacc gatgacatgc gagcgagctc ttcgtagtcg acgacttcgg tcgacctact
      601 cactagtgga cgccgccgat ggcttcgccg acctttgcgc acgtcggccg caccgcgggt
      661 gaccgcgtgc tcagcagcga gctccagtgg tcgcagccgc gccttgtcgg cagtgacctc
      721 gtcaggagta tcggaacacc gtctgcgcag caccccgcaa gagatcggcc acggcaccaa
      781 actgccgccg ccggtggtcg actcgagcgc gaaaggcatc ggctgcggtc gacgtccaga
      841 tcgcttcagc cgcagcccgc accagttgcg cgcccgccat ctctacctgc accgcgcgct
      901 ggtcgagttc aagctcgatc tcgtcgatgt tcgacaccac aaccaccgtc cgccgtagca
      961 gtgccgactg cgcgccggtg ggacgaacct acgagcggtc ggtcacctca cggttgacca
     1021 tcgagacttc actggaaagg gccgacggct gtggagagtc aagccgtacg gctcggacgt
     1081 cgacccgtac cctcatcgca tgccatggct gcccgcggac ttcgtgcacc cgctacgcgc
     1141 ggaggtcgac gccgagcacc acctgaggcc gattcgggca accgacgtcg accttgacat
     1201 ggtcgctgtc atgagctcgc aagcacgact gtggtcgatc ttcggcccag cctggcagtg
     1261 gccgccgcct tctatgacca aggagcagga ccgcgctgac ttggcccgac acgagctgga
     1321 gatcgaagcc tatcagtcgt tcaactacgc gctgttcgac tctgcgcaaa ccgcgctgct
     1381 cggctgtgtc tacatcgatc ccccagagaa acacggcgcg gacgccgaga tctcgtggtg
     1441 ggtaatcgat cagcttctcg acagtgagac cgagacgtct ctgaacgagc tcgtgccgcg
     1501 atggatcgcg acgcactggc cgttcacaaa cccacgctac atcggtcgcg acatcatgtg
     1561 gacggactgg cttgatctgc cggacgccga ccaaccgtcg taactacgca cccgcgcgcg
     1621 ccgccgcctt catctctttc ttgtgctcac gaaccttttg caatgactcc gagctggtga
     1681 catcagcgac tgaccggtag ctgtcgtcct cgccgtacgg gcccgcggcc tcgcgccaac
     1741 cacgcggccg caccccgcgc tgtttcccca acagagcaac gaaaatccgc gccttttgcg
     1801 caccgaaccc gggcaacgtc gccaatcggg cgtgcagctc tgcgccggac ttcgcgtcgt
     1861 cccacaccgc ctcggcgcgg ccctcgtagt ccgcgaccaa gagacgagcg agcgcctgca
     1921 cacgaccggc catcgcgccg ggaaaacggt ggattgcggg tggccccaca aacagcgtgg
     1981 cgaacgtcac ggggtcgtgc tcggccatgt cgtacgcgtc gaggtggtcg acccccaacc
     2041 gctgcgcgag caggtatgga ccggcgaacg cccgctccat cgggaactgc tggtcgagca
     2101 gcatcccgac gagcagggca agcggatcac ggtcgagcaa tgcgtcggcg tccggctgtt
     2161 gggcgatatg aagagtcatg gggaagctcc gttcggtcga tactcaagcc ttgcagattc
     2221 tccgcgccct gggaaaggcg gtcagtacca cccgccggta gggagctgct cgccgactgg
     2281 aacatcaacg tcgagcacgt tgccaggcgg cgccagcggg caggcccatg cggcgtcgta
     2341 cgcgcacgat gggtggtacg caaagttgaa gtcgagcacg atcgtgcccg ctgctgcatc
     2401 gcttccgagg tcggcgccct tggcggtgtc gatcaggtag cggccaccgc cgtacgtcgt
     2461 gcggttcgcc gtccgatcgc gtagtggaat gaacagaccg ccgccatagc tgcgcagtgc
     2521 ccatacgtcg agctgtccga cgtccgggat acgaaccacg ccggtccggt cgaacggcac
     2581 gatgccgtcg gtgcccgtgg cgatctcgat gtgcgacggc tcgatgtcgg tgtccatcgg
     2641 gagctcgaag cgccacgccg ggtcgtaggc tttgaccggg acgccggtga atgcggcacg
     2701 acgatcgact gcaatcggcg actgcggatg atgggcgaac aggttgtcgc gctcagctga
     2761 ccaacttgcg tgggccgctg gacggtcggt ggccgcccgg acggcggtat agacggcgtg
     2821 cacgcggcgc cgccagtcga gcagcgtcaa gcccgcgtgg tccataccgg cagtgtgcgg
     2881 cctactgctt gtgtagggcc agttgggacg cggccgcgat gatcgcggca ccggtcatgt
     2941 ccagcgccgg ctcgtcggtc tgccacgacc gcacgtcgtc gacgaagctc gccgtcgagg
     3001 tgtcgaacgc cgcgaacttg tcgacgccgg tcgtcggaca tttcgccatc ccgtcctgca
     3061 aaccgccgag gccgccctcg aagttggcgt tgtcgttggg accgttcacg acggcaccca
     3121 catcgagggc agggccgacg ccgtgcaggt tgccgctgag gtttgccacc tggtgctgca
     3181 tgcagtcagg gaaggtcgag ccctcgccga ccatgaagct ggtaccccag gcgttcgcac
     3241 cgaagagcca gtcgcgctgc tcggccgcga acgcgtcgaa gcggtggtcg ccggtcagtt
     3301 cggcgtacca ggcctccatg gcgatccacc cgaacgtgtg cgagtcgacg tcgaactgat
     3361 tgatgtcgcc ggccgcgcga aaggtgtcac cggcggcatg cgtggcgccg agttccactt
     3421 gacgcttcag gtccttgatc agagcggcct tgctgacggc aagcgtcggg ttgcccgcgg
     3481 cggccatcgc gtgaaccaga tcggtgtgtg cgagcgcgct cgtgtcgtag aggttgaacg
     3541 tgtcgcccgc tgccgcgtcg tccgcgatgt agttcttcgc gaaacccgcc gcagcgacca
     3601 ggtatggcat cgcgttgtgc gacagtttct gagcagccag cgcgatttcg gccccgccga
     3661 gctccatgtc gtcgtgccac gtcgtctccg ggtagtacgc ctcaggatcg gcagtgacga
     3721 gcggggacgg cgggtggctg gtgttcgcca tcgcgtacag cgacgttgcc tcgaggtatt
     3781 cgtactgcgc gcgcgcaggg ttggtcacag cgtcgacctg cgcggccaac gcgaaggctg
     3841 ccgacacccg cccgaccagg ttcggactga tctggtggcc cggtgcagca gcctcgaaga
     3901 ccggacggtc gactgttgcg taacggtcca tcgggtcggt gtcggcggcg tcggcctgcg
     3961 ggagccgcca caagtcgtgg tcgccgttga acgtcgggtc gacggtgtcg ctgccgttcc
     4021 ccgtgccgac gcccacctgc aggtacagcg tcttcgtcga catctgccac atcttgtcga
     4081 gccagttctc gccgtacgcc gcctcggtct tcagggtgga cggcgcagag ttgcccaacg
     4141 cacgctcgct cgcgtacagc accacgtcgc cgtacgcggc agtgtgcgtg aacttcagga
     4201 agtcgccggc gtcataccaa ccgccctcgg cgttgaccgt gggcgcgccc ttgatcttga
     4261 tgagcggcga gtcgggcacc agggagtcac cgctggtcgt gtcgacgaag tcgggggttt
     4321 ggtaaacggt ggcgtgcgcg tcgcccagat gagccggctg ccggtccagt ggcccaggga
     4381 cgacgtcggc gccgtcccgc tgcacctgat agaagttgac gccgtcgagg acgacctgac
     4441 tgtagaggct ggccgcgctc tcgatcgcga aggacggtga ctgtgcagtc gcatcgccgt
     4501 gcacggccag gtgataggtg ccgagcgcgc cgagcccact aaacgtgatg tcgtacacgt
     4561 cgggatagtt cgtgttccac ggcccgcggt tggtggtacc gacatggcca tgggcaacaa
     4621 cgtggttcgc ggcgtcgaca acatcccatg tggcgtgtgg cacccgctgg ttcgccatca
     4681 ggtacgcgtg cttcgcatct gtcggcaggt agccgacctg gtcgacgcgc acgagggccg
     4741 cgggtgccga ctggctggcg acggcgtcgg ctggcgcggc cgcaagtgat ccgagcgcaa
     4801 cgccggcgaa cagcacggtg gccgccggaa ggctgatcca cgtcgaaggg cgccgaaaga
     4861 ctgtgcgacc catgatcgtc acctgactgt taggaagcct tcctaccgac accgcagggt
     4921 aggtcgcggc atcggcgtcg tcaagagacc ggcgcccact cacgaccgca ccctgcgaac
     4981 ctccgaaggg cctcgcacaa agcggacaat cggccctcac ggaattccat cagaagtacg
     5041 tcccatcaac ccgctgacct gcgggtttac cagtgttcca gcgggcttga ttcgtgaaac
     5101 tcggtgccta taactggctc gtgctcgcca cctcccgcgc cactcaatta ctcgccgtgt
     5161 tcctcgcggt ggtctcctcg ttgcccgacg ccgaggcggc cctggatgcc ggcgtcgaag
     5221 gtgctgcgct cgcgctggag gcgcaggttg ccgcggcggt ctgcggcggc cgggctctga
     5281 cgtcggtggc tatcccgcgg acgagatacc gcacgaagaa ctggctccgg tcgcggccgg
     5341 cgaccggcag accctcgtcg tgcccaccgc ccatcggccc gcgtgcgcga ttgcagttcc
     5401 ggtgggttct ctagacggac acctcgtcgt cgcgtgtcgg tgacgaggct ttcagcaccg
     5461 aggaagtaaa ccttgcgcgc ggcatggcga gggccatgga catgaccttc cagatgctgc
     5521 gcacgcttga ctccgagcga cagttgcgcg accgcagcga acgtcaggca gcggaaatgc
     5581 ggcactgctt gtctcgctgc gcgcaaggca aaggctgtct gaggagctat ccgcgatcca
     5641 gcgcgcgatt tcgcggcgcg acccacttgc cgacatcctc gacgcggtca cccggggcgt
     5701 ccgcgatctg ctcggtgaag agatcgcggt gcttcgtcaa ggtgcaccga cgttgtcgcc
     5761 gaagcagtcg tcgtccgagc cccggctagc ggcggaatac cagatcgtat cggcaactgg
     5821 cgggcccggc gaactggcga aatggctggc gaccgtgccg atgcccgacc acctcgcgcc
     5881 tggccccggc tcgtggctgt cgtccgacga cggtgcgcag ctgggcacgc ttccggaccc
     5941 tcgcgccggc gtccaatccc tccggtcgcc ttaggtgctg ggcttcgccg cgaagtccag
     6001 gtcggccgta gtgacggtgc ccgcgctacg ccccggagcc gtgtggtact tccaatacag
     6061 gttcgtgtgg gcgatgacct tgtcgggcgg cggtgccccc cactgcgact ggtcttcggt
     6121 cgtgtgagca tcgctgacga gcgtggcgtc gtagccgcgg acgatcgcac cgtgaagtgt
     6181 cgatcggatg cattcgtcgg tctgcgcgcc cgagataatc agctggccga tgccgcgcgc
     6241 cgcgagtacc gactcgagat cggtctcttc gaacgaatcg gcgtacgcct tctgcaccaa
     6301 cacctccgac tcccgccgaa cgagctcggg cacgtactgc cagccctcgc tgtctttcgg
     6361 cagatcatcg ctgttgtgct ggacccagac gacatcgacg tcggccgccc gtgccttgtc
     6421 gacgagggtg gcgatattgg cgaccacggc gtctcggtcg tacgcctccc ccacgatgcc
     6481 gttctgcacg tcgatcacga tcaacgcggt gttcggtcga ttctcgagcg tggacatggg
     6541 cgtctccctg catcggactg atctggcgtc tggctgacct acggtccgag caggctatgc
     6601 ccgcccaacg acaagcactc gagatggcaa gctggaggca tgagcgaatc cgatcccaag
     6661 gcagacttgc ggcgttacct gcaggtcgcc cgcgaggcag ttctgtggaa actcgacgcg
     6721 ctgtcggagt acgagatccg tcggccgatg acccccgccg gcaccaacct gttgggcctg
     6781 gtcaaacatc tggctaacgt cgaggtcggg tatttcggcg agacgttcgg caggccgttc
     6841 gacgggcctc tcccatgggt ggaggggccc gaaggcgagg agcccgatcc gaacgctgac
     6901 ctatgggcca ccgtcgacga gtcgcgcgac cagatcgtcg ggctgtacca ggccgcgtgg
     6961 gcacacgcgg acaagacgat cgagacgctg gacttggcaa cgatcggcgc agtgccgtgg
     7021 tggcctgccg accacgcgga ggtcaccctg cacaaggtct tggtgcacgt catcgccgag
     7081 acgaaccggc acgccggaca cgccgacatc gtccgggaac tgatcgacgg agccaccggg
     7141 ttgcgcgcgg gcaaccacaa catggcggac ggcgaccaag cgtggtggca ggagtaccgg
     7201 aaccggttag agcaggccgc ccgggacgcg tcgccggaac aactccacga ctgacgcttc
     7261 cgcgaaaggg ctagatacaa ccgagcgacg cgttggagcg tcgattcgtc atggtcgtga
     7321 gccgaaatgc cgtcgctgcg acgttgactg gtgtgtgcgt tgcggcggcg ctgcagctca
     7381 gcgcctgcga cagcgtcgcc tccaggcaac gctcttcggg cgtcaccggg ccatcaacga
     7441 ggccggcgtc ggcgccagcg tcgtccgacg cagcctcgtc cccagcgacc atgccggcga
     7501 gccaacctgc gacgtccagc gggccgccat cggcgtgcgc tctgccgaca ccggacggcg
     7561 gctggggcac ctggctgcgg gccgcacagt tcgtctcacc gacgaaagga tgggtcgtcg
     7621 gcaccgccgg catcctggcc acgactgacg gcgcgcattg gtcgtggcaa tacggcgcgg
     7681 ccacggcggc ctttgacggc gtcgacttca tcgactcgga gcacggctgg gcggttggtc
     7741 cgaacacgct tgtgcgtacg accgacggcg gcacccactg gcaacaactc accga
//
LOCUS       DATCBJ010000472         2379 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_47568, whole genome shotgun sequence.
ACCESSION   DATCBJ010000472 DATCBJ010000000
VERSION     DATCBJ010000472.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2379)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2379)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2379
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_47568"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..672)
                     /locus_tag="VII50_09560"
     CDS             complement(<1..672)
                     /locus_tag="VII50_09560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BadF/BadG/BcrA/BcrD ATPase family protein"
                     /protein_id="HEY5051138.1"
                     /translation="MSVLPVLGVDAGGSATRAVLVDDGEVLARFEEPPLNVLLQADAF
                     ERLVALIRGSGAAAAGLGLAGLRGREHAAEIGRRLAAATGVDVLVVDDTEAALAGAFG
                     DRPGIVVVAGTGSNALGRGGDGSIVRAGGYGFLLGDEGSAYWFAGQALRAALRSHDGS
                     GRKSGALEAAVTAEYGLDFDGIVRLVYSAPADRQLLARLAHTVMALENDPVMADILDA
                     GCDYLV"
     gene            998..1753
                     /locus_tag="VII50_09565"
     CDS             998..1753
                     /locus_tag="VII50_09565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEY5051139.1"
                     /translation="MPARSQTLDESPNGEPRVPKYYLLKRHLLDMTRTMPPGSPVPPE
                     RTLSVDFGTSRTTVRQALQELVVEGRLERIQGKGTFVAKPKVAQELRLTSYTEDMRAQ
                     GLEPTSTLLEIGYVLADEVLAERLAIKRSARVLRIERLRLANGEPMAIETTHLAGQRY
                     PGLRRLLARSTSLYAVLAQEYGIELLEAEETIETVPAPPREAHLLGTDVGLPLLLLSR
                     HSRDSAGQPIEYVRSLYRGDRYKFVAHLQRPTS"
     gene            complement(1757..>2379)
                     /locus_tag="VII50_09570"
     CDS             complement(1757..>2379)
                     /locus_tag="VII50_09570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HEY5051140.1"
                     /translation="EVHIVTVEDPIEVLHFDKLGMINQREVRVDTEDFSTALRAAMRQ
                     DPDVILVGEMRDQETVKAALAASETGHFVMSTLHTTDAQETVNRIIDFFPPHEQKQVR
                     LSLASALRGIVCQRLVPRADGEGRCVTMEICINTGRIAEAIADPDKTSLMNDLIAEGA
                     YYGMQTFDQHLVALIRDGAVTLEAAMSASTNPHDLTVELRRLGLVA"
BASE COUNT          422 a          798 c          771 g          388 t
ORIGIN      
        1 gacgaggtag tcgcagccgg catcgaggat gtctgccatg acggggtcgt tctccaacgc
       61 cataaccgtg tgggcaagcc tggccaacag ctgacgatct gcgggagcgc tgtagaccag
      121 ccgcacgatc ccgtcgaagt cgagcccgta ctcggctgtg actgcggcct cgagcgcccc
      181 actcttgcgc ccgctgccgt cgtgggagcg cagggcggcg cgcagtgcct gaccggcgaa
      241 ccaataggcg cttccttcgt ctcccagcaa gaacccgtaa ccgccggccc gaacaatgct
      301 gccatcgccg ccccggccga gtgcgttcga gccggtgcct gcgacgacga cgatgccagg
      361 ccggtcaccg aaggcgccgg ccagtgcggc ctcggtgtca tcgacaacga ggacgtcgac
      421 gccggtcgcg gccgccagcc gtcgtccgat ctccgcggcg tgctcgcgac cgcgaaggcc
      481 agcgagcccg aggccggccg ccgccgcgcc cgagcccctg atcaaggcca ccagtcgttc
      541 gaaggcatcg gcctgcaaca acacattcag cggcggttct tcgaagcggg cgaggacttc
      601 cccgtcatcg accagaacgg cccgagtcgc agatcctccg gcgtcgacgc cgagcaccgg
      661 tagtactgac attcgttctt ctcccgtcag cccttgaccg cacccgcggt gagcccggcg
      721 ccgaaatgcc cctgcagcac atcctgctcg aaatcacggt ggaccagcac tcccgcgcca
      781 gcagattggt gtagaccatt ccgtaagttg gcatagacca cttgtactgt caaggggttc
      841 acgccacagc ggtgccggtc gtttcgcaac cgtgatgaaa cgacgatccg agcgcgacgc
      901 gagaagcgtt gaagggtacg tggtctagac ctctattgaa ccatcactat gatgggagaa
      961 acccggatcg gtccaccact gcgaggttga ggcgaacgtg ccggcaagat cgcaaaccct
     1021 cgacgaatcg ccgaacggcg agccacgggt gcccaagtac tacctgctca agcggcatct
     1081 gctcgacatg acgcgcacga tgcccccggg tagcccggtg ccgccggagc ggaccctcag
     1141 cgtcgacttc gggacgtcgc ggacgacggt tcgacaggca ctacaggaac tcgtggtgga
     1201 agggcggctg gagcggatcc agggcaaggg caccttcgtt gccaagccga aggtcgcgca
     1261 ggaactgcgc ctgacgagct acaccgagga catgcgtgcg cagggcctcg aaccgacatc
     1321 gacgctcctg gagatcggct acgtactcgc cgacgaggtt ttggccgaac ggctcgcgat
     1381 caagcggagc gcccgggtcc ttcgcatcga gcggcttcga ctcgctaacg gcgagccgat
     1441 ggcgatcgag accacccacc tcgcgggtca gcggtacccc ggccttcggc ggctgctcgc
     1501 ccgctcgacg tcgttgtacg cggtcctggc ccaggagtac ggcatcgagt tgctcgaggc
     1561 cgaggaaacc attgaaacgg tgcctgcccc gccgcgcgag gcgcatctgc tcggcaccga
     1621 cgtcggactg ccgttactcc tgctaagccg ccacagccgc gactcggccg gccaaccgat
     1681 cgagtacgtg cggtcgctgt atcgcggtga ccgctacaag ttcgtcgccc acctgcaacg
     1741 tccaacgagc tgagacctac gccaccaggc ccaggcgccg tagctcaacg gtcaggtcgt
     1801 gcgggttcgt cgacgcgctc atggcggcct cgagagtgac cgcgccgtcg cggatcagcg
     1861 cgaccaggtg ctggtcgaag gtctgcatgc cgtagtaggc gccttcggcg atgaggtcgt
     1921 tcatgagcga agtcttgtcg gggtcagcga tcgcttccgc gattcggccg gtgttgatgc
     1981 agatttccat cgtgacgcag cgcccttcgc cgtccgcccg aggaacgagc cgctggcaca
     2041 cgatgccgcg caatgcactc gcgagggaga gccgaacctg cttttgctcg tgcggcggga
     2101 aaaagtcgat gatgcggttg accgtctcct gcgcgtcggt ggtgtgcagg gtcgacatca
     2161 cgaagtgccc ggtctcggac gccgcgagcg ccgccttgac cgtttcctgg tcgcgcatct
     2221 cgccgacgag gatcacgtcg gggtcctgcc gcatggcggc acgcagcgca gtggagaagt
     2281 cttcggtgtc gacgcgaacc tcgcgctggt tgatcatgcc gagcttgtcg aagtgcagca
     2341 cctcgatcgg gtcttcgacc gtgacgatgt gtacttcgc
//
LOCUS       DATCBJ010000473         8080 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_423850, whole genome shotgun sequence.
ACCESSION   DATCBJ010000473 DATCBJ010000000
VERSION     DATCBJ010000473.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 8080)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8080)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8080
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_423850"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..449
                     /locus_tag="VII50_09575"
     CDS             <1..449
                     /locus_tag="VII50_09575"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008929 - methylglyoxal synthase activity
                     [Evidence IEA];
                     GO_process: GO:0019242 - methylglyoxal biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HEY5051141.1"
                     /translation="GEQRHIALVAHDNKKLDLLNWADFNRLTLSKHVLYATGTTGTML
                     EHELGLHVHRFLSGPVGGDQQIGAKIAEGVIDLLVFFWDPLEPQPHDPDVKALLRIAA
                     VWNIGVACNLASADLMISSPLLDGPYERFRPDYGDVPSSTILAAVS"
     gene            488..1213
                     /locus_tag="VII50_09580"
     CDS             488..1213
                     /locus_tag="VII50_09580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009076479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008276 - protein methyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative protein N(5)-glutamine
                     methyltransferase"
                     /protein_id="HEY5051142.1"
                     /translation="MRAAGCVFAEDEAELLIAAAPAASDLARLVAQRVEGLPLEQVLG
                     WAEFCGLRMVIDPGVFVPRRRSEFLVRQAADVARPGAVVVDMCCGSGAVGAALAAVQR
                     IELHAADIDPVAVQCARRNVDGNVYQGDLYEPLPVTLRGCVDVLVANAPYVPTDEIAL
                     MPPEARLYEPQVALDGGVDGLDIQRRVVVGAPFWLAPAGTLLIETSDRQAARTAEFVA
                     RAGMQPRVVRSDEFDATVVIGTS"
     gene            complement(1206..2258)
                     /locus_tag="VII50_09585"
     CDS             complement(1206..2258)
                     /locus_tag="VII50_09585"
                     /inference="COORDINATES: protein motif:HMM:NF044836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I mannose-6-phosphate isomerase"
                     /protein_id="HEY5051143.1"
                     /translation="MEPVSLPANQPQQFYLGGPQIEAFRGQPSAAGSHPTSIFPADPA
                     RTPEDWIASTLSKFGSLDGATRLPDGELLSDAVTAHPEEFFSPAHIARFGANPALLVK
                     LLDAGERLPVHCHPDRAFAGVHLGSVFGKTEAWIIIGTPGADAHVYLGFNREVEPAEL
                     SNWFINQNSAEILDSMNRVAVQTGDAIFVPAGTPHAIGAGAFIVELQEPTDFSVMLEW
                     REFGMPDRRDEQLGMSAELSLQCFNRHQFTDADLALCHRHAEPDHGGTVALFPAQAEP
                     FFQAHEIIVTDFADLAAQYAVLIVVGGTGKLTTPTSELELTRGSTVLIPYSAGPTTLR
                     GTLHAIRCLPGDPALS"
     gene            2321..3769
                     /locus_tag="VII50_09590"
     CDS             2321..3769
                     /locus_tag="VII50_09590"
                     /EC_number="3.2.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011718972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008422 - beta-glucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0030245 - cellulose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH1 family beta-glucosidase"
                     /protein_id="HEY5051144.1"
                     /translation="MYGPTMCTQAKEHAKQMMRTFPAAFTWGSATAAYQIEGAVAEDG
                     RTASIWDTFSHTPGKVLAGDNGDVAADHYHRYAADVDLMSELGMGSYRFSVSWPRVIP
                     TGSGAVNPKGIDFYSRLVDRLLAKGISPALTLYHWDLPQELDDAGGWTARDTAYRFAD
                     YAAAVAVALGDRVPLWTTLNEPWCSAFLGYAGGVHAPGHTSGAEALAATHHLLLGHGL
                     ALQQLRAAVSPSAKLAITLNPAMARPATPSADDAAACAKIDGLQNRIWLDPLFHARYP
                     SDVVEFTSGLCDWAFVHDGDLATMATPFDVLGVNYYSPVIVGHFGGDGERARADGHGN
                     TIGNTWPGCEDTQFIDLPGRHTSMGWPVDATGLHELLTRLWADYRKPMLITENGAAYE
                     DEVSADGAVHDPERVRYIHEHLVALHDAIADGIDVRGYYLWSLLDNFEWSYGFSKRFG
                     IVRVDYESQLRTPKDSARFYAEVVRANAVPPR"
     gene            3841..5481
                     /locus_tag="VII50_09595"
     CDS             3841..5481
                     /locus_tag="VII50_09595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009077635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HEY5051145.1"
                     /translation="MTETARTTARWWREAVIYEVYVRSFADSDGAGVGDLRGITSRLP
                     QLAELGIDAVWLTPFYRSPMADGGYDVADYEDVDPTLGTLEDFDVLLASAHDLDLKVI
                     VDLVPNHTSNKHAWFQAALAAGPGSPERARYIFRDGRGPDGNEPPADWLANFGGSAWT
                     QVADGQWYLHMFAPEQPDLDWSNPVVGTAFESILRFWLDKGVDGFRIDVAHGMAKNLT
                     PPLPDLGAKSVDRQLRYEVVDHPLWDRDEVHDIFRVWRRILDEYSPPRIGVAEAWVMP
                     GRRARYVRPDELHQAFNFDFLQTPWGCAALVEVIEASLREAGAVGATATWVLSNHDVV
                     RHLSRYALPSSVDLRPWLLSNGTVPAPDLAAGLRRARAAALLMLALPGSSYLYQGEEL
                     GLPEVADIAPQYLQDPTWERSDHHDKGRDGCRVPLPWEPTGPSFGFSSVSGWIPQPPD
                     WAQLSWGAQDGDPASTLELYRSALALRRKFGTDDALTWLTKGPATLSFARSNGLVCIV
                     NFGSEPIAIPAGQLLLASGELADGGLLPTDAAAWVLTN"
     gene            5539..6306
                     /locus_tag="VII50_09600"
     CDS             5539..6306
                     /locus_tag="VII50_09600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009083564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEY5051146.1"
                     /translation="MTTSRPDADGLAVGRILVVDDDPTVSDVVARYLRREGYEVSLVG
                     DGLSALESAAADSPDLVVLDIMLPGLDGLAVCRTLRERSPVPVILLTALGAETDRIMG
                     LEVGADDYVTKPFSPRELVLRVASVLRRARTPAPLRVSERAGLIDGPLRVDLAAHEVE
                     YGGLPLMLTVREFDLLAHLMGHPRKTFRREELMRDVWGWSFGDQSTVTVHVRRLRQKL
                     EAASDGNWSPISTVWGVGYRYDPSPVLAPGVSELAAE"
     gene            6320..7555
                     /locus_tag="VII50_09605"
     CDS             6320..7555
                     /locus_tag="VII50_09605"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEY5051147.1"
                     /translation="MVLHPHDVGVLVAVSAGSSVVVTAIGLGLLRAARRRPLRDAALI
                     VALTPVFTVVVAASAATMWMFLSKRQFGVLIATVVFAGIGGIVTSMVLARTLMRGSDA
                     LRAAANALARGEPYVGPIGTPSAELEALAAELEAAHAKLDEARRHDRAVEDSRRELIA
                     WVSHDLRTPLAGIRAMAEALEDNVVFTDEDRALYHRRIRVEVDRLARMVDDLFELSRI
                     HAGALQLSPRPMALTDIVREAVATAAPVASAKGIQLVASGLEAAGPLVDVDPYALTRV
                     IGNLLGNAIRHTPPDGSVDIAMVHDDDFVYLAIGDECGGIAEDDVDRVFEVAFRGTPA
                     RTPAPDGGAGLGLAIARGIVNAHNGRIGAENVGPGCRFTIALPRARNAPTTVTPTGHT
                     FDPADQPNGRIRGDAQSLT"
     gene            7888..>8080
                     /locus_tag="VII50_09610"
     CDS             7888..>8080
                     /locus_tag="VII50_09610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5051148.1"
                     /translation="MLLAELVTVSGAVAATRSRSAKIALVAGCLRSAHSSQVGDEVEI
                     AAAFLSGELRQRRTGIGWAA"
BASE COUNT         1301 a         2542 c         2754 g         1483 t
ORIGIN      
        1 ctggcgaaca acgccacatt gcgctcgtag cgcatgacaa taagaagttg gatctgctca
       61 actgggcgga cttcaaccgg ctcacgctgt ccaagcacgt gctctacgcg acaggcacca
      121 ccggcacgat gttggagcac gagctcggct tgcacgtgca ccggttcctg tccggtcccg
      181 tcggcggcga tcagcagatc ggcgcgaaga ttgccgaagg cgtcatcgat ttgctggtgt
      241 tcttctggga tcccctcgag ccacagccgc acgatcctga cgtgaaagcg ctgctgcgta
      301 ttgcagcggt ctggaacatc ggcgttgcct gcaatctcgc aagcgcagac ctgatgattt
      361 cctcgccact gctcgacggt ccgtacgagc ggttccggcc cgactacggt gacgtgccgt
      421 cgtccacgat tctggctgcg gtcagctgaa cccgtcgtct gcggatcggt ccgcggttgt
      481 cgccagcttg cgcgcggctg gttgcgtctt tgccgaggac gaagcggaac tgttgatcgc
      541 cgccgcaccg gcggcgtccg accttgcacg gctcgtcgcg cagcgtgttg aagggctgcc
      601 acttgagcag gtgctcgggt gggcggagtt ctgcggcctt cgcatggtga tcgacccagg
      661 cgtgttcgtg ccgcgtcgcc ggagtgagtt cctggtccgg caggccgcgg acgtcgcgcg
      721 accgggcgcc gtggttgtcg acatgtgctg tggctcgggt gcggtaggcg ccgcactcgc
      781 cgccgtgcag cgcatcgaac tgcacgccgc cgatatcgat ccggtcgcgg tgcaatgcgc
      841 tcggcgcaac gtggacggca atgtttatca gggcgacctc tacgaaccac tcccagtgac
      901 cttgcgcggt tgcgtcgatg tactcgttgc aaacgcgccc tacgtaccta ccgacgagat
      961 tgctctcatg ccgccggagg ctcgcctcta tgaaccgcaa gtggctctgg acggcggggt
     1021 ggacggcctc gatatccagc gacgggtagt tgtgggcgcg ccgttttggc tcgcacccgc
     1081 aggcacgctc ttgatcgaga cgagcgaccg acaagcagcg cgcaccgccg agttcgttgc
     1141 gcgagccggg atgcaaccgc gcgtggtcag gtccgacgaa ttcgatgcga ccgtcgtcat
     1201 cgggactagc tgagcgcagg gtctcccggc aagcatcgga tcgcgtgcaa tgtgccgcgc
     1261 agagtggtcg ggccggcgga ataggggatc agcaccgtcg agccgcgagt gagttcgagc
     1321 tccgacgtcg gggtcgtcag tttgcccgtt ccgccgacta cgatcaacac cgcgtattgg
     1381 gccgccaagt cggcgaagtc cgtcacgatg atctcgtggg cttggaagaa cggctcggcc
     1441 tgtgcgggaa acaacgcgac ggtgccgccg tggtcgggtt ctgcgtgccg gtggcagagt
     1501 gcgagatcgg cgtcggtgaa ttgatgccgg ttgaaacact gcagcgacaa ctccgcgctc
     1561 atgccgagtt gctcgtcacg tcgatccggc atgccgaact ctcgccattc gagcatgacg
     1621 gaaaaatcgg tgggttcctg aagttcgacg atgaaggcgc cggcaccgat ggcgtgcggc
     1681 gtcccggccg gtacgaagat cgcatcaccg gtctgcaccg cgacgcggtt catcgagtcg
     1741 aggatctccg cgctgttctg gttgatgaac cagttgctca actcggcggg ctcgacttcg
     1801 cggttgaagc cgagatagac gtgtgcgtcg gcacccggcg tcccgatgat gatccaggcc
     1861 tcggtcttcc cgaacacgga gccaaggtgg acgccggcaa acgcacgatc gggatggcag
     1921 tgcaccggca agcgctcgcc ggcgtcgagc agcttcacca gtagcgcggg gttggcgccg
     1981 aagcgggcga tgtgtgcggg gctgaagaac tcctccgggt gcgcagtgac cgcgtcgctg
     2041 agcaactcgc cgtccggcaa tcgggtggcg ccgtccaagc tgccgaactt cgacagggtg
     2101 gacgcgatcc agtcctccgg tgtgcgggcc gggtctgcag ggaagatcga ggtagggtgc
     2161 gagcccgcgg ccgatggctg gccgcggaag gcctcgatct gcgggccacc gaggtagaac
     2221 tgctgcggct ggttcgcggg caggcttact ggctccatca gggcgcgctc ctggctaggc
     2281 tcaactcagg tcgccgccca ccgtagcggg agcgggtgcg gtgtacggcc cgaccatgtg
     2341 cacgcaggcg aaggagcatg ccaaacagat gatgcgaacc tttccagccg ccttcacgtg
     2401 gggctctgca accgccgcat atcagattga gggagccgtc gccgaagacg gccgcaccgc
     2461 gtcgatctgg gacaccttca gccatacgcc gggcaaggtt ctcgctggcg acaacggtga
     2521 cgtcgccgct gaccattacc accggtacgc ggccgacgtt gacctgatgt cagagctggg
     2581 aatgggctcg taccgcttct cggtgtcctg gcctcgcgtg atcccgacgg ggtctggcgc
     2641 ggtcaacccg aaggggatcg atttctactc gcgccttgtc gaccggctgc tcgccaaagg
     2701 aatctcgccg gcgctgaccc tgtaccactg ggatctcccg caggaattgg acgacgccgg
     2761 cggttggacc gcgcgcgaca ccgcctaccg tttcgccgac tacgcggccg cggttgccgt
     2821 agccctcgga gatcgagtgc cgttgtggac aacgctgaac gagccgtggt gctcggcatt
     2881 cctcggctac gccggtggcg tgcacgcgcc cggccacacg agcggcgcgg aggcgttagc
     2941 ggcaactcac cacctgctgc tcggacacgg tctcgcgctc cagcaactgc gtgccgcggt
     3001 gtcgccgtcc gccaagttgg cgatcacgtt gaacccggcg atggcgaggc cagcgacgcc
     3061 gtcggccgac gacgccgcag cgtgcgcgaa gatcgacggc ctgcagaacc ggatctggct
     3121 cgacccgctg tttcatgcgc gctacccctc cgacgtcgtc gagttcacgt ctgggctttg
     3181 tgactgggca ttcgtgcacg acggtgacct cgcgacgatg gcgaccccgt tcgacgtcct
     3241 cggggtgaat tactacagcc cggtgatcgt cgggcacttt ggcggggacg gcgagcgggc
     3301 tcgggccgat ggccacggca acacgatagg caacacctgg cccggctgtg aggatacgca
     3361 gttcatcgat ctgccaggaa ggcacacgtc gatgggctgg ccggtcgacg ccaccggttt
     3421 gcatgagctg ctcaccaggc tctgggccga ttaccgcaag ccgatgctga tcaccgaaaa
     3481 cggtgcggca tacgaagacg aggtgtcggc tgacggcgcg gtgcacgacc ccgaacgggt
     3541 tcgttacatc catgagcatc ttgttgcatt gcatgacgcc atcgcggacg gcatcgacgt
     3601 tcggggctac tacctatggt cgctgctgga caatttcgag tggtcgtacg gcttttccaa
     3661 gcggttcggg atcgtccgcg tcgactacga aagccagctg cggacgccga aggacagtgc
     3721 gcgcttttac gcggaggtcg tccgggctaa cgccgtgccg ccgcggtaag cccacgggca
     3781 ctgcgtaggc tgcagtcgcc ggctaaatcc tgcgccgcgg cacaacactt tggagttttc
     3841 atgaccgaaa cggcacgcac cacggcgcgc tggtggcgtg aagccgtgat ctatgaggtc
     3901 tacgtgcgta gcttcgccga ctccgacggc gctggcgtcg gggacctgcg cggcatcact
     3961 tcgcggttgc cacagctcgc cgaactcggt atcgacgccg tctggctgac gccgttctac
     4021 cggtcgccca tggccgacgg cggctacgac gtcgccgact acgaggatgt cgatccaact
     4081 cttggcaccc tcgaggactt cgacgtctta ctcgctagtg cgcatgacct ggacctcaag
     4141 gtcatcgtcg acctggttcc gaaccacacc tcgaataagc acgcgtggtt tcaggcggcc
     4201 ctcgcggcag gaccaggatc gccggagcgg gcgcgctaca tcttccgtga cggccgcggt
     4261 cccgacggta acgagccgcc tgccgactgg ctggcgaact tcggtggctc ggcgtggacc
     4321 caagtggccg acggccagtg gtacctgcac atgttcgcgc ccgagcagcc cgatctcgac
     4381 tggtcgaacc ctgtcgtcgg cacggccttc gagtcgattc ttcggttctg gctcgacaag
     4441 ggagtcgatg gcttccgcat cgatgtcgct catgggatgg ccaagaattt gacgccgccg
     4501 ttgccggacc tcggtgcgaa gtcggtcgac aggcagttgc gttacgaggt ggtcgaccat
     4561 ccgctctggg accgcgacga ggtgcacgac atcttccggg tctggcggcg gattctcgat
     4621 gaatacagcc cgccgcggat cggggttgcc gaagcgtggg tgatgcccgg tcggcgggcg
     4681 cgctacgtcc ggccggacga attgcatcag gccttcaact tcgatttcct ccagacaccg
     4741 tggggctgtg cggcacttgt cgaggtcatc gaagcgtcgc tacgtgaagc cggcgcggtc
     4801 ggggccaccg cgacgtgggt gctctcgaat catgacgtgg tgcggcacct gagtcgctac
     4861 gcgttgccgt cctcagtcga ccttcggccg tggctgttga gcaacggcac ggtgcccgcg
     4921 cccgacctcg ctgcgggcct gcgccgggca cgcgcagcgg ccctcttgat gcttgcgctg
     4981 cccgggtcct cctacctcta tcagggtgag gaactcggac tgcccgaagt cgccgacatc
     5041 gcgccgcagt acctgcagga cccaacgtgg gagcgctcgg atcatcacga caagggccgg
     5101 gacggttgcc gggtgccgtt gccatgggaa ccgaccggtc cgtcgttcgg cttcagctct
     5161 gtcagcgggt ggattccgca gccaccagat tgggcgcagc tgtcctgggg tgcgcaggac
     5221 ggcgacccgg catcgacgct cgagctttat cgcagcgcgc tggcgttgcg acgaaagttc
     5281 ggcaccgacg acgcgctgac ctggctcacc aaaggtccgg caaccctgtc gttcgctcgc
     5341 tcgaacggtc ttgtctgcat cgtcaacttc gggtccgagc ctattgcgat ccccgctgga
     5401 cagctgttgc tggcaagcgg cgaactcgcc gacggcggcc tgctccccac cgatgccgct
     5461 gcctgggttc ttacgaacta gagaacgcgg gcgccgcagg acagggggtc caccaggtgc
     5521 ggatctaggc tgaatctcat gacaacttca aggccggacg ccgacgggct ggcggttggc
     5581 cggatcctgg tcgtcgatga cgacccgacg gtcagtgacg tggtcgcgcg atacctccgg
     5641 cgcgagggct atgaggtgag cctggttggc gacgggctgt ctgcgcttga gtctgcggcc
     5701 gcggactcac ccgatctcgt cgtgctcgac atcatgttgc ccggcctcga cggccttgcc
     5761 gtttgccgca cgctgcgtga gcgcagccca gttccggtga tcctgctgac agcgctaggc
     5821 gccgagaccg accgcatcat gggtctcgag gtcggcgcgg acgattacgt gacgaaaccg
     5881 ttcagcccgc gcgaactcgt gctgcgggtg gcatcggtgc tgcggcgagc tcggacgccg
     5941 gcccccttgc gggtctcgga gcgtgccggc ctcatcgacg gacccttgcg tgtcgacctc
     6001 gctgcgcacg aggttgaata cggtggtttg ccgctgatgc ttacggttcg cgaattcgac
     6061 ctgctcgcac atctgatggg acatccgcgc aagacatttc gtcgcgagga actgatgcgc
     6121 gacgtctggg gctggtcgtt cggtgatcag tcgacagtga ccgttcacgt gcgccggctg
     6181 aggcagaagc tcgaggccgc ttcggacggg aactggtcgc cgatctcgac ggtgtggggc
     6241 gtcggatatc gctacgaccc gagcccggtg cttgcacccg gagtgagcga gctggcagcc
     6301 gaatgattgc gacgcagtga tggtgttgca tccgcacgac gtcggtgtat tggtcgcggt
     6361 atccgcgggc agctcggtgg ttgttaccgc gataggactc ggcctgttga gggcagcgcg
     6421 tcgacgtccc ttgcgcgatg ccgcgctgat cgtcgccctc acgccggtgt tcaccgtggt
     6481 tgtcgctgcg agcgccgcga cgatgtggat gttcttgtcg aagagacagt tcggcgtcct
     6541 cattgcgacg gtggtgttcg ccggaatcgg cggcatcgtc acctcgatgg tgctggctcg
     6601 caccttgatg cggggcagcg atgcgctgcg cgcagcggcg aacgcgctgg cgcgtggtga
     6661 gccttatgtg ggcccgatcg ggacgccgag tgccgagctc gaggcgctcg cggccgagct
     6721 cgaagccgcg cacgcgaaac tcgacgaagc ccgtcgtcac gatcgcgcgg tcgaggacag
     6781 ccgccgcgaa ctgatcgcct gggtgagcca cgacctgcgc acgccgcttg ccgggattcg
     6841 ggcgatggcc gaggccctcg aagacaacgt cgtgttcacc gacgaggatc gcgcgctgta
     6901 tcaccgcagg atcagggttg aggtcgaccg gcttgccagg atggtcgacg acctgttcga
     6961 gttgagcagg atccatgccg gcgcactgca gttatcgccg cgcccgatgg cattgaccga
     7021 cattgttcga gaggccgtcg ccacggccgc ccctgtggct agcgcgaaag gaatccaact
     7081 tgtcgcgtca ggtctggagg cggctggccc gcttgtcgac gtcgatccct atgcgctgac
     7141 cagggtcatc ggcaatcttt tgggtaacgc gatccggcat accccacccg acggttcggt
     7201 cgatatcgcc atggttcacg acgacgactt cgtgtatctg gcgatcggtg acgaatgcgg
     7261 tggtatcgcg gaagacgatg tcgaccgggt gttcgaggtc gcgttccgcg ggacgccagc
     7321 ccggacgccg gcgccggacg gtggcgccgg cctcggcctc gcgatcgcgc gcggcatcgt
     7381 gaacgcacac aacggacgaa taggtgcaga gaatgtcgga ccgggatgcc gattcaccat
     7441 cgccttgccc cgcgccagga atgccccgac gacggtgact ccaacagggc atacgttcga
     7501 ccccgcagac caaccgaacg gacgtatccg gggggatgcc cagtcgctaa cttaaacgtg
     7561 tcacatagag ggggtggccc gtggggggct ttacgtagtg ggcgcgtgtt gagtggattc
     7621 aggggcaacg gccgatgtgg cgtgagcacg acgcaaagct tgcgtccgtg tggcgtcgtg
     7681 aagcgtcgga cactgcttcc cctgttgcca ctgacaccgt gatcgtgcta cccgaacctg
     7741 tggagcctgt cgcgcagagc gcctaggcaa taccggctca tgtcgtcggt gaccgcgctg
     7801 gggggcagcg gtcaccgacg acagccattt gtcggagcct gtagatggct gtcggcgact
     7861 gtcagtgact gtcgcaagaa tgaccccgtg ttgcttgccg agttggtcac cgtgtccggc
     7921 gccgtcgccg cgacccggtc gcgatcggcc aagatcgccc tcgtcgctgg ttgtcttcgg
     7981 tcagcgcaca gtagtcaggt cggcgatgag gtcgagatcg cggccgcatt tctttccggg
     8041 gagttgcgcc agcggcgtac cggtatcggc tgggcggcgc
//
LOCUS       DATCBJ010000474         4853 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_158014, whole genome shotgun sequence.
ACCESSION   DATCBJ010000474 DATCBJ010000000
VERSION     DATCBJ010000474.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 4853)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4853)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4853
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_158014"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..549
                     /gene="ruvX"
                     /locus_tag="VII50_09615"
     CDS             <1..549
                     /gene="ruvX"
                     /locus_tag="VII50_09615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HEY5051149.1"
                     /translation="CGAGNDSPACLERVSEPAAVMTGVDIRPGVRLGIDVGSVRVGVA
                     ASDPGAVLAAPVETVRRGDDRATTRDDLRRIAALVAERGAVEVVVGLPLSMNGTRGTA
                     ARAALVYAQAIASAVAPVPVRVVDERLSTVTAHGQLGAVGVRSRARREVVDQAAAVII
                     LQSALDAERSSGRAPGELVTAP"
     gene            558..1700
                     /gene="mltG"
                     /locus_tag="VII50_09620"
     CDS             558..1700
                     /gene="mltG"
                     /locus_tag="VII50_09620"
                     /inference="COORDINATES: protein motif:HMM:TIGR00247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HEY5051150.1"
                     /translation="MDPFDLGLRHGEPKPALYRRLLQVPFAIIASGLVVIAIVVGAVN
                     VTGKVFHPQASNDYTGDGYGSVQVSVHPGQALTAIGETLVQAGVVKSVTAFSNATSDN
                     AAARSIQPGTYQLRLQMSAASAVALMVSPDSLVGGRVTIPEGLRISRVEQLVTQGDAT
                     IKVADIQAALARPTDFGLPAYANGQAEGFLYPATYNVDNTTTATNLLVQMVGRFNQVA
                     ATINLAQGAAALRLTPYQVVIIASLIEAEVKRPQDFPQVAEVIINRMHKGMPLELDST
                     VNYALDTTKPFLSPTDLKTASPFNTYLHTGLPPTPIDSPGQDALLAALNPAQGNYLYF
                     VTTDPTTGDTTFTASLTEANALRVLAQKNAAAIAAGSAPASAPATP"
     gene            1725..2588
                     /locus_tag="VII50_09625"
     CDS             1725..2588
                     /locus_tag="VII50_09625"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HEY5051151.1"
                     /translation="MSSPTAVPALRRAAVLGSPIAHSLSPVLHAAAYQILGLRWSYLA
                     IECDEAQLPGVLLELDESFVGLSLTMPLKRAVLPLLDDVSELAAAVGAANTVVFDGVG
                     PFLRRRGENTDVPGMVAAIAARRPDGVRSAVVLGAGGTAAAALAALRELGVDRGVIVV
                     RDQARTGEIRRCADRLGVTPTLVDWPGRAAFGEADVIISTVPAGATDALATQALVGPG
                     QLLFDVLYEPWPTAFAAASQAAGATVIGGLELLVQQAARQVELWTGRPAPVEAMRAAG
                     EAAIASRSVGS"
     gene            2676..3914
                     /locus_tag="VII50_09630"
     CDS             2676..3914
                     /locus_tag="VII50_09630"
                     /inference="COORDINATES: protein motif:HMM:NF015232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="roadblock/LC7 domain-containing protein"
                     /protein_id="HEY5051152.1"
                     /translation="MSSVQQPLEYVSRASRLDRILRELLHQTPGVEAAAVVSFDGLPM
                     ASALPTGMDEDRVAAMSAALLSLAERAAQGLGRGDLSQVYIEGENGTVFLVSAQDEAV
                     LIAVTAADAKVGMMLYEVKLAASHVGEVLRGPVPLPAPLPSHVTRGYLDAHDSGSNSS
                     GSNSSGSNGASGGYDGYAAQAEAYETTAPDASLADLPPSDSAYGEDEDPSPLYSELSH
                     AESYSEPSYAETLYTETSNAETSNAEPTLDEDAAENTAAATEELADAARVDIEFAADE
                     SLGTQGETDDSAGPETAIDDVSSDAAVTDADDEEDDESDEVYPDRVYTEIVYTEASLT
                     QPASTEPADAQSDDDSDEGEVAHAETAPTTPSYSEPSYYAEPSYYAEPAAPAAPPAEQ
                     PGWSSYAPSGSVLPGGQAWS"
     gene            4032..>4853
                     /locus_tag="VII50_09635"
     CDS             4032..>4853
                     /locus_tag="VII50_09635"
                     /inference="COORDINATES: protein motif:HMM:NF025690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4388 domain-containing protein"
                     /protein_id="HEY5051153.1"
                     /translation="MKLEGSLDAFSLPDIFQLLSFTKKSGGLHLRRATTRGSVYFRDG
                     AVTGASSDDGRQALARRLVGSAGVGDNELTAAVERAATEGIGVSRALLDAGAVDPDFL
                     RSLVAEQVVDAVFDLLRWSEGDFSFTVDEPGPDDVGISLGVEPVVNDARSRLEAWDHA
                     CRVVPSPETVLALPVGVREDPVVTRGEWALLALVDGRRTVAELVALAGRGDYAVVSQL
                     AALIERGLLKVRTQDAGEGALALARRQEILGRLENERVAAEVVDVDAILVPPATIF"
BASE COUNT          786 a         1597 c         1645 g          825 t
ORIGIN      
        1 tgcggtgctg gcaacgattc accagcgtgt cttgagcgcg tgagcgagcc tgcggccgtt
       61 atgaccggcg tcgatattcg gccaggagtt cggctgggaa tcgatgtggg cagcgttcgc
      121 gtcggagtgg cggcgagcga tcccggcgcg gtgctcgctg ccccggtcga gaccgtgcgg
      181 cgtggtgacg accgggccac cactcgcgac gacctgcgcc gtatcgcggc gcttgtcgcc
      241 gagcgcggcg cggtcgaggt cgtggtcggc ttgccattgt cgatgaacgg cacccgcggc
      301 acggcagcgc gcgctgcgct tgtgtacgcg caggcgatcg cttcggcggt cgcgccggtg
      361 ccggtccggg tggtcgacga gcggttgtcg acggtcaccg ctcacgggca gctgggtgcg
      421 gtcggggtcc gctcgcgagc gcgccgcgag gtcgttgacc aggcggcagc tgtcatcatc
      481 ttgcagagtg cccttgacgc cgagagatcg tcgggccggg cacccgggga gctggtgacg
      541 gcaccgtgag ttcgccgatg gacccgtttg acctcggcct gcgccatggc gagccgaagc
      601 cggcgctgta tcgccggctc cttcaggtgc cgttcgcgat cattgcctcg ggcctggtgg
      661 tcatcgcgat cgtggttggt gccgtcaacg tgacaggcaa ggtcttccac ccgcaagcat
      721 cgaacgatta caccggtgac ggctacggct cggtccaggt ctccgtgcac ccgggccagg
      781 cgctgacggc aatcggtgaa acgctcgtcc aggctggtgt cgtgaagagc gtgaccgcat
      841 tcagcaacgc gacgagcgac aacgcggcgg cgcgatccat ccagcccggc acctatcaac
      901 tacgcctgca gatgtccgcc gcgagtgccg ttgcgttgat ggtgtcgccc gactctttgg
      961 ttggtggccg ggtgacgatt cccgaaggcc tgcgcatcag ccgcgtcgag cagctggtca
     1021 cccaaggcga tgccacgatc aaggtcgccg acatccaggc cgccttggct cggccgacgg
     1081 acttcgggct gcccgcttac gcgaacggcc aagcagaggg ctttctctat ccggcgacgt
     1141 acaacgtcga caacacgacg acggcgacta atttgctcgt ccagatggtg ggacgcttca
     1201 accaggtcgc cgcgacgatc aacctcgctc agggcgccgc ggcattgcgt cttacgccct
     1261 accaggtcgt gatcatcgcg agcctgatcg aggcggaggt gaagaggccg caggacttcc
     1321 cgcaggtcgc cgaggtcatc atcaaccgga tgcacaaggg gatgcctctg gagctcgact
     1381 cgaccgtgaa ctacgcgctg gataccacga agccgttcct gagcccgacg gacctgaaga
     1441 cggcctcgcc cttcaacacc tacctgcaca ccggcctgcc gccgacgccg atcgactccc
     1501 cagggcagga cgcgctgctc gctgcgctga atccggccca gggcaactac ctgtacttcg
     1561 taacgacgga tccgacgacc ggtgacacga ccttcactgc gtctttgacg gaggcgaacg
     1621 cgttgcgggt gctcgcgcag aagaatgcgg ccgccatcgc ggcaggttcg gcacccgcct
     1681 cggctccggc cacgccgtga ggcagcgacc ttgagccgtc aaccttgagt tcgcccaccg
     1741 ccgtccctgc cctgcgccgc gcggccgtgc tcggctcgcc gattgcgcat tcgctgtcgc
     1801 ctgttctgca cgccgccgcg taccagatcc ttggtctgcg atggagctac ttggcgatcg
     1861 agtgtgacga ggcgcagctg ccgggtgtcc ttctcgagct cgacgagagc ttcgtcgggc
     1921 tgtcgctcac catgccgctg aaacgggcgg tcctgccgtt gctggacgac gtcagcgagc
     1981 tggccgccgc ggtcggtgcg gcgaataccg tggtgttcga cggggttggg cccttccttc
     2041 ggcggcgcgg tgagaacacc gatgttcctg gcatggtggc cgcgattgcg gcccggcgcc
     2101 cggacggcgt ccgcagcgcc gtcgtgctcg gcgcgggcgg cactgccgcc gcggcgctgg
     2161 ccgcgctgcg cgaacttggc gtcgaccgag gcgtcattgt cgtgagagac caggctcgaa
     2221 ccggcgagat ccggcgttgt gcggaccgtc tcggagtgac gccgacgctg gtcgactggc
     2281 caggccgggc agcattcggc gaggcggacg tgatcatttc gacggtgccc gcgggtgcca
     2341 ccgatgcgct cgcgacgcag gcacttgtgg gccccgggca gttgctcttt gacgtcctgt
     2401 acgagccttg gccgactgca ttcgccgcag cctcgcaggc cgccggcgcc acggtgatcg
     2461 gcgggcttga gctccttgtt cagcaggctg cccgccaggt cgaactctgg accggtcggc
     2521 ccgccccggt cgaagcgatg cgggcggccg gcgaggcggc gatcgcatcg cggtcggtgg
     2581 gctcgtgagg ttcaagccca ccccccgggc cgccgatggg ttaccggaag acccgtacaa
     2641 tcccgggtca gacgggtcct gagagaaggt gctccatgtc ctctgtgcag cagccgcttg
     2701 agtacgtgag tcgggctagt cggctggatc gaatcctgcg cgagttgttg caccagaccc
     2761 ctggtgtcga agctgccgcg gtggtctcct tcgacggact acccatggct tcggcgttgc
     2821 ccaccgggat ggacgaggat cgcgtcgccg ccatgagcgc agccctgctc tcactggccg
     2881 agcgggccgc ccaaggcctt ggtcgcggtg acctgtcgca ggtctacatc gaaggcgaga
     2941 acggcacggt gttcctcgtc tctgcgcagg acgaagccgt actcatcgcg gtcaccgcgg
     3001 cggatgccaa agtcggcatg atgctctacg aagtgaagct ggccgcaagc catgtcggcg
     3061 aggtgttgcg tgggcctgtg ccgctgcccg caccactgcc gtcgcacgtc acgcgcggtt
     3121 acctcgacgc acacgacagt ggatcgaata gcagtggatc gaatagcagt ggatcgaacg
     3181 gcgcgtccgg tggctatgac ggatacgcag cgcaagctga ggcgtacgag accaccgcgc
     3241 ccgacgccag ccttgccgac ctccccccca gcgattctgc gtatggcgag gacgaggatc
     3301 cctcaccgct gtacagcgag ctcagccacg ccgagtcgta ttccgagccg tcgtacgcgg
     3361 agaccttgta caccgagact tccaacgccg agacttccaa cgccgagcca acacttgacg
     3421 aggacgccgc ggagaacacc gcggctgcca ctgaggagtt agcggacgcc gcacgagtcg
     3481 acatcgagtt cgcggcagac gaatcccttg gtacgcaggg cgaaaccgat gacagcgcag
     3541 gaccggaaac ggccatcgac gacgtaagtt ctgacgctgc ggttaccgat gccgacgatg
     3601 aagaggacga cgagtccgac gaggtctatc ccgaccgcgt ctacaccgag atcgtctaca
     3661 ccgaagcatc gttgacgcag cctgcgtcga ccgaaccggc cgacgcacag agcgacgacg
     3721 acagtgacga gggcgaggta gcgcacgcag aaacggcccc aactacgccg tcgtacagcg
     3781 aaccgtcgta ctacgccgaa ccgtcgtact acgccgaacc cgctgcgcca gccgcgcccc
     3841 cagcagaaca gcctggctgg tcgtcctacg cgccgagcgg cagcgtgctg cccggcggac
     3901 aggcatggtc gtagcggtcg tcctggcata gctcgtcgcc ggcgccgtgt cctacttcca
     3961 cctgcaaaag gtcgcgcagc tgcaactttc gtcgtcccac tggccacact cgtcaccgga
     4021 aaggcagacc cgtgaaactt gaaggctcac tcgatgcctt cagccttccg gacatcttcc
     4081 agctcttgag ctttacgaag aagtcgggtg ggttgcatct tcgacgtgca accacgcgtg
     4141 gctcggtgta cttccgcgac ggcgcggtga ccggggcgtc ctccgacgat ggtcggcagg
     4201 cactggcgcg ccgattggtc ggcagcgcag gcgtcggcga caacgaactg acggccgcgg
     4261 tcgagcgcgc ggctactgag ggaattggcg tgagccgggc gctgctcgac gcgggcgcag
     4321 tcgatcccga tttcttgcgg agcctcgtcg ccgagcaggt tgtcgatgcc gtgttcgatc
     4381 tactgcggtg gagcgaaggc gatttctcct tcacggtcga cgaacctggc cccgacgatg
     4441 tcggaatttc actcggcgtc gagcccgtcg tgaacgacgc acgcagccgt ctcgaagcat
     4501 gggaccacgc gtgtcgggtg gtcccgtcac ccgagaccgt cctcgcactg ccggtcgggg
     4561 tccgtgaaga cccggtggtg acgcgcggtg agtgggcgtt gctggcgttg gttgatggac
     4621 gccgaaccgt ggccgaactg gtggcacttg ccggtcgcgg cgattacgcc gtggtctctc
     4681 agctcgccgc tctcatcgaa cgcgggctgc tcaaggtgcg aacccaggac gctggcgagg
     4741 gagcccttgc gctggctcgc cgccaagaaa tcctgggtcg actggagaat gagcgagtcg
     4801 ccgccgaagt cgtcgatgtc gacgcgattt tggtgccgcc cgcaacgatc ttt
//
LOCUS       DATCBJ010000475         3742 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_109843, whole genome shotgun sequence.
ACCESSION   DATCBJ010000475 DATCBJ010000000
VERSION     DATCBJ010000475.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 3742)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3742)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3742
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_109843"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..376
                     /locus_tag="VII50_09640"
     CDS             <1..376
                     /locus_tag="VII50_09640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5051154.1"
                     /translation="AQDSIDAPIDGFMMSKKVKNQAGATALLEYIGTAQAEALYGKTD
                     SSDVAAATNADTSNYNPIQTASAALIKNSKNIAQFLDRDTNPDFADNTVAAMIQSFFN
                     NPGQVSSQLKALEGQAKTVFTS"
     gene            376..1329
                     /locus_tag="VII50_09645"
     CDS             376..1329
                     /locus_tag="VII50_09645"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEY5051155.1"
                     /translation="MVAATQAIGSSPAVRKRRRRGVRLLSAGDRVVLSLMVFIPLAIV
                     IFFVWFPAIATVFLSFTTWSGIGGLKAIRADGIQNYHQIATNYPPFIPALEHNVLWLV
                     VFILFATPLGILIAVLLDQKIRGSRIYQSLLFLPVMLSLALIGIIWELMYSNNYGLIN
                     SLIGRNGQTNFIDWLGNPKLNIWAVLVEASWRQVGYVMVLYLAGLKAVDPTLREAATV
                     DGASPWQTFWRVVFPVLRPINVVIIVITVIESLRAFDLVYITNGGTNGLELLSVLVTR
                     NILGQQSRIGFGSSLGVVLLVIALVPIITFLVQQLRQEKRR"
     gene            1326..2240
                     /locus_tag="VII50_09650"
     CDS             1326..2240
                     /locus_tag="VII50_09650"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEY5051156.1"
                     /translation="MTVTASAGQLIPAATVAHRRQSERKHVGSHVFLIIMTVLWTTPL
                     LWAMYTSLRPYSETAKYGYLSLPHHLTFSNFTTAWTQADIVHYFWNSVIITLPAVVFT
                     LGLASCVAYVLARFSFWFNIPLLIIFVSANLLPPQVIITPLYRIYLTIHVPHLISSSG
                     LLYNSYAGLIVVNTAFQLGFCVFVLSNYMKTIPNEITEAALVDGASVWTRYWKLTLPL
                     CRPALAALATLLTTWIYNDFFWAITLISSGNKRPITSALANLQGQFFINQNLLAAGAL
                     ITAVPTLLVYVLLQKQFIAGLSLGSSKG"
     gene            2245..>3742
                     /locus_tag="VII50_09655"
     CDS             2245..>3742
                     /locus_tag="VII50_09655"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: protein motif:HMM:NF014156.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HEY5051157.1"
                     /translation="MSDPTANPTWVTLRLDGVMVVFDVGGPHLPRIVHWGSDLGDLSP
                     ADDARLPAAAFFLIDDSTHNADMPLTVSPTRAQGWSGWPGLSGHRDGTASQPLFVQSG
                     LETDESADGHALRYRGTDDAAALQLDGEFVLTPEGVLRHRQTVTSTAASDRSSYVVDD
                     LLTLLPVSEHVAEVFDHAGRWANEAQGQRQTLHQGTWLREQRRGRTGPDSPLLLMVGT
                     EGFDYRDGQVWGIHLGWSGDQRYLAQRLNTGAVMLGAGEILAAGEVILGAGESITTPW
                     AYGVYSGAGIDGASRRLHTMLRRRPSHPHRPRPIVLNTWEAVYFDHSFDRLAALADVA
                     AQIGVERFVIDDGWFGSRRDDRSGLGDWYVSAQVWPDGLGSIADYVRSRGMEFGLWFE
                     PEMVNLDSDVARAHPDWVIGTPGRMPPPFRGQQVLDVSQPDAFAYLLERLSSLVGEYG
                     ISYIKWDHNRDIADPVHRGGPRAGRPAIHDQTLATYRLMAQLRDLHPGL"
BASE COUNT          639 a         1172 c         1139 g          792 t
ORIGIN      
        1 cgcccaggac tccatcgacg ccccgattga tggattcatg atgagcaaga aggtgaagaa
       61 ccaggccggc gccaccgcgc tcttggagta catcggtacc gctcaggccg aggcgctcta
      121 cggtaagacc gactcgagtg acgtggcggc tgcgacgaac gccgacacga gcaactacaa
      181 cccgatccaa actgcgtcgg ccgcgctgat caagaactcg aagaacatcg cccagttcct
      241 tgaccgtgac acgaaccctg acttcgcgga caacacggtt gcggccatga tccagagctt
      301 cttcaacaac ccagggcagg tttcatcgca gctcaaggcc cttgaagggc aggcaaagac
      361 ggtgtttacc agctgatggt cgcagctact caagcgatcg gctcctcgcc ggcggtgcgc
      421 aagcgccgcc ggcggggagt tcgcttgttg tcggccggcg acagggtggt tctgtcgctg
      481 atggtcttca ttcccttggc catcgtcatc ttctttgttt ggttcccggc aatcgcgacg
      541 gtgttcctgt cgttcaccac gtggagcggc atcggcggcc tcaaggcgat ccgtgccgac
      601 ggcattcaga actatcacca gatcgcgacg aactacccgc cgttcatccc ggcgctggag
      661 cacaacgtgc tttggctcgt cgtgttcatc ctcttcgcca ctccgcttgg gattttgatc
      721 gccgtccttc tcgaccagaa gattcgcggc agtcgcatct atcagagcct gctcttcttg
      781 ccggtcatgc tgtcgcttgc gctgatcggg atcatctggg agctcatgta ctcgaacaac
      841 tacggtttga tcaacagctt gatcggccgt aacgggcaga ccaacttcat cgactggctc
      901 ggaaatccga agctgaacat ctgggctgtt ttggtcgaag catcatggcg ccaggttggg
      961 tacgtcatgg tgctgtattt ggccggtctg aaggcggttg acccgacgct gcgtgaggcg
     1021 gcgacggtcg acggtgcgag tccgtggcag acgttttggc gtgtcgtgtt tcctgtgttg
     1081 cgcccgatta acgtcgtcat catcgtcatt accgtgatcg agtcgttgcg cgcgttcgac
     1141 ctcgtgtaca tcacgaacgg tggcacgaac ggcctcgagc tgttgtctgt gctggtcacc
     1201 cgcaacatcc tggggcaaca aagccggatc ggcttcggct cgtcattggg tgtcgtgttg
     1261 ctcgtcatcg cgctggtacc gatcatcacc ttcctcgtcc agcagctgcg tcaggagaag
     1321 cgccgatgac ggtcaccgcg tccgcgggtc agttgatacc tgcagctacc gttgctcatc
     1381 gccgccagtc cgagcgcaag catgtcggat ctcacgtttt cctgatcatc atgaccgtgc
     1441 tatggacaac accgctgctg tgggcgatgt acacatcgct gcggccgtac agcgagacgg
     1501 ccaaatacgg ctacctgtca ctgccgcacc atctgacctt ctcgaacttc acaaccgctt
     1561 ggacgcaagc cgacatcgtc cattacttct ggaactcggt gatcatcacg ttgccagcgg
     1621 tggtattcac tcttggcctt gcgtcttgcg tcgcctacgt gctggcccgc ttcagcttct
     1681 ggttcaatat cccgctgttg atcattttcg tgtcggctaa cctgcttcct ccgcaggtga
     1741 tcatcacgcc gctctaccgc atctacctga caatccacgt gccgcatttg atcagcagca
     1801 gcgggctgct gtacaactcc tacgccgggt tgattgtcgt gaacaccgcg ttccagctgg
     1861 gattttgcgt tttcgtgctg agcaactaca tgaagacgat tcccaacgaa atcaccgaag
     1921 cggctctggt cgacggcgca tctgtatgga cacgctactg gaagctgacg ctgccgctgt
     1981 gccgtcctgc cctggccgcg ctggcgactt tgctcacgac ctggatctac aacgacttct
     2041 tctgggcaat cacattgatc tcatcgggga acaagcgacc gatcacgtcg gcactggcca
     2101 acctgcaggg ccagttcttc atcaatcaga acctgctcgc cgccggtgcg ctcatcacgg
     2161 cggtgccgac acttctggtc tacgtgctcc tgcaaaagca gttcatcgcg ggcctgtcgc
     2221 tcggttcgtc taagggctga gctgttgagc gatccgactg ccaacccgac ctgggtgacg
     2281 cttcgcctcg atggcgtgat ggttgtgttc gatgtcggtg gtccgcactt gccgcgcatc
     2341 gtgcattggg gtagcgacct cggtgatctg agcccggccg atgacgcaag gctgccggca
     2401 gccgcttttt ttctgattga cgacagcacc cacaacgccg acatgccgct cacggtctcg
     2461 ccgactcggg cacaaggttg gtcgggctgg cccggccttt ccggtcatcg cgacggaacg
     2521 gcgtcgcagc cgttgttcgt gcagtcgggg ctggaaaccg acgagtcagc cgacgggcac
     2581 gcgttgcgtt atcgaggtac cgacgacgcc gccgccctgc agctggacgg cgagttcgtg
     2641 ctcaccccgg aaggtgtgct gcggcaccgg cagacagtga ccagcaccgc ggcgtccgat
     2701 cgttcgtctt atgtcgttga tgatctgctc acgctgctgc ctgtctcgga gcacgtcgcc
     2761 gaggtgttcg accatgcggg gagatgggcg aacgaagcgc agggtcagcg gcaaaccctg
     2821 caccagggaa cctggttacg cgagcagcgg cgcggccgca ccggacccga ttcgccgttg
     2881 ttgctgatgg ttggcaccga gggtttcgac taccgcgatg gtcaggtgtg ggggattcac
     2941 ctcggctggt caggcgacca gcgctacctc gcgcaacggc tgaacaccgg tgcggtgatg
     3001 ctcggcgcgg gggagatcct cgcggccggt gaggtgatcc tcggtgctgg cgaatccatc
     3061 actacgccgt gggcctacgg cgtgtactcg ggtgcgggta tcgatggcgc ctcgcgacgg
     3121 ctgcacacga tgctgcggcg gcgaccgagc catccgcacc gtccgcgacc gatcgtgctc
     3181 aacacctggg aagccgtgta cttcgaccat tcgttcgacc ggctggcggc actcgccgac
     3241 gtcgcagcgc agatcggcgt cgagcggttc gtcattgatg acggctggtt cgggtcgcgt
     3301 cgtgacgacc gctccggcct cggcgactgg tatgtgtcgg cgcaggtatg gccggacggt
     3361 cttggctcga tagctgacta cgtgcgatcg cgaggcatgg agttcgggct ctggttcgaa
     3421 cccgagatgg tcaacctcga ctccgacgtt gctcgcgcgc acccggactg ggtcatcggg
     3481 acgcccggtc ggatgccgcc accatttcgc ggccagcagg tgctcgatgt atcccaaccg
     3541 gacgcgttcg cctatctgct ggagcggctg tcgtccctgg ttggcgagta cggcatcagc
     3601 tacatcaagt gggatcacaa tcgcgatatc gccgacccgg tgcaccgcgg cggaccgcgc
     3661 gccggtcggc ccgcgatcca cgatcaaacg ctggccactt accggctcat ggcgcaactg
     3721 cgcgatttgc accccggcct gg
//
LOCUS       DATCBJ010000476         3269 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_10251, whole genome shotgun sequence.
ACCESSION   DATCBJ010000476 DATCBJ010000000
VERSION     DATCBJ010000476.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 3269)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3269)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3269
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_10251"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..490
                     /locus_tag="VII50_09660"
     CDS             <1..490
                     /locus_tag="VII50_09660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="molybdopterin-binding protein"
                     /protein_id="HEY5051158.1"
                     /translation="VTTGGVSAGAYDTVKAVLSRLGTVEFLKVAMQPGMPQGFGTVGP
                     DSTPIFTLPGNPVSAYVSFEVFVRPAIRRMLGVLPLYRPLVRARLAEPITKTAGKRAY
                     VRARLDVNDGAYVVTPVGGMGSHLVADLALANAFIVAPDHVSEIPTGATVSVMMLERR
                     QT"
     gene            487..960
                     /gene="moaC"
                     /locus_tag="VII50_09665"
     CDS             487..960
                     /gene="moaC"
                     /locus_tag="VII50_09665"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003791807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HEY5051159.1"
                     /translation="MTDFSHLDARGAARMVDVSGKDITARTATASGRVLVSGEVVALL
                     RGGGVPKGDALAVARLAGIQGAKRTPDLVPLAHPIALHGVTVDLEVSDDSVRIVATAR
                     TADRTGVEMEALTAVAVAGLALIDMVKAVDPGAVITDIRVDEKTGGSHGIWSRPT"
     gene            1128..1643
                     /locus_tag="VII50_09670"
     CDS             1128..1643
                     /locus_tag="VII50_09670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MogA/MoaB family molybdenum cofactor
                     biosynthesis protein"
                     /protein_id="HEY5051160.1"
                     /translation="MAPPTPRALVITASDRASGGMYADKGGPVIIDALQAIGFEADGP
                     IVVPDGAPVEVALRSGLEAAYDVIITTGGTGLGPRDHTPQMTAQVLDYEVPGIAEAIR
                     AAARVDVPTAVLGRGVAGVAGRTLIVNLPGSTGAVRDGMAVLAPILQHAVEQLAGSNH
                     DRGGAEHHGGG"
     gene            1650..2279
                     /locus_tag="VII50_09675"
     CDS             1650..2279
                     /locus_tag="VII50_09675"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HEY5051161.1"
                     /translation="MSVGWPVTLEHGPVKLRPLRLRDAGEWRMTRLLNREWLEPWEAT
                     TPGVTPSDAGRGYGDAVRALRRDARLGLCYPFALLVGEQFAGQVTVGAIARGSANSAY
                     LGYWIARQFAGRGIMPTALAMVIDQCFGDAGLHRVEANIRPENAASRRVVEKLGFRLE
                     GTRERYIHIAGAWCDHLSYALTVEDVPGGLLPRWLAAGGGARLSSADTT"
     gene            2351..>3269
                     /locus_tag="VII50_09680"
     CDS             2351..>3269
                     /locus_tag="VII50_09680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5051162.1"
                     /translation="MSSLIVLGIVGIWILVLVPMWLNRHDADSASRSMDTFSTAMRVL
                     SRRGPSRGRFARSARADRIADGQYLVMPRDNRGVTVDNQTDTSTGQRLPRLRVPLHLP
                     SRRRSYATSGRAQVIARRRRIAVGFAVLVIGLALSAEFAHTSWWFELGACLALAGYLI
                     HLRTEAIRTVEMRRRRLARVARAAAPYPTARTAGGEHYYAARVRHDADIEPAATDAVS
                     AAGAVSASGTEDGSRWEPIAVPLPTYVSKPVVSRPAVAAPRADVESGPTIDLTQPGKW
                     IDSQVDVRYLLLDDDVAEVDELDVILARRR"
BASE COUNT          482 a         1094 c         1143 g          550 t
ORIGIN      
        1 cgtgaccact ggcggtgtga gtgcgggtgc ctacgacaca gtgaaagcgg tgctgtcgcg
       61 cttgggcacg gtcgagttct tgaaggtcgc gatgcagccg ggcatgccgc agggcttcgg
      121 cacggtcggc cccgattcga ccccgatttt cacgctgcca ggcaatccgg tgagcgcgta
      181 tgtgtcgttc gaagtctttg ttcgcccggc aattcgtcgc atgctcggcg tcctgcctct
      241 gtatcgccca ctcgttcgcg cgcggttggc ggagccgatc accaagaccg ctgggaagcg
      301 cgcgtacgtg cgcgcgcgtc tcgacgtcaa cgacggcgcg tacgtcgtga cgccggtcgg
      361 tggcatgggc tcgcacctgg tcgccgacct cgcgctggcc aacgcgttca tcgtcgcgcc
      421 cgaccacgtc tccgagatcc cgaccggcgc gaccgtgtcg gtgatgatgc tcgagcggcg
      481 ccagacgtga cggacttttc gcacctggac gctcgcggcg cggcacgcat ggtcgacgtc
      541 tcaggcaaag acatcacggc gcggacggcg acggcatcgg gtcgtgttct cgtgtctggg
      601 gaagtcgtcg cgttgctccg cggcggtggg gtgcccaaag gagacgcgct cgcggtagcg
      661 cggctggcgg ggattcaggg ggcgaagcgc acgcccgatc tggtgccgct ggcgcacccg
      721 atcgcgctgc acggggtgac cgttgacctc gaggtttcag acgattcggt tcggatcgtg
      781 gccaccgccc gtaccgccga ccgaaccggc gtcgagatgg aagcgctgac cgccgtggcc
      841 gtcgcaggcc tcgcgctgat cgacatggtg aaggcggtcg atcccggcgc ggtgatcacc
      901 gacattcggg ttgacgagaa gaccggcggt tctcacggca tctggtcccg cccaacctga
      961 ccagcccggt ctccgcttcg cctcccaggg tgtcttttgc agcggccgga ggagctttcc
     1021 cccggctgcc aaggacccga cggggaacgg ccgattgccc cacgcgcgca cccttgccgc
     1081 gatgcactag tttttcgtca tgctgaccgt cgcaaagcgc tgagccgatg gcgccgccca
     1141 ccccaagggc gctggtcatc accgcgtccg accgcgcgtc tggcggaatg tacgccgaca
     1201 agggcggtcc ggtgatcatt gacgcgctgc aggcgatcgg gttcgaggcg gacggtccga
     1261 tcgtggtacc cgacggcgct ccggtcgagg tcgcgttacg gtcgggactc gaggcggcct
     1321 acgacgtcat catcaccact ggaggcacgg gtcttgggcc gcgcgaccac actccgcaga
     1381 tgaccgccca agtgctcgac tacgaggtgc cggggatcgc ggaagcgatc cgggcggcgg
     1441 cacgggtcga cgtcccgact gctgttcttg gtcgtggcgt tgccggtgtc gccggacgca
     1501 cgctcatagt gaatctgcct ggatcgacgg gcgcggtgcg ggacgggatg gcggttttgg
     1561 cgccgatcct ccagcatgct gtcgagcaac tcgcggggag caaccatgat cgcggtggcg
     1621 ccgagcacca tggcggagga taggcgtttg tgagcgtggg ctggccggtc actcttgagc
     1681 acggcccggt gaagctgcgg ccgttgcgcc tccgcgacgc cggcgagtgg cgcatgacgc
     1741 ggctgctgaa tcgcgagtgg ctcgaaccct gggaagcgac gacgccgggg gtcacgccat
     1801 ccgatgctgg gcgcgggtac ggcgacgccg ttcgagcact ccgacgcgac gcccggctcg
     1861 gcctctgcta cccgttcgcc ctgctcgtcg gggagcagtt cgccggccag gtcacggttg
     1921 gcgcgatcgc gcgcggttcg gcgaactccg cgtacctcgg ctactggatc gctcgccaat
     1981 tcgccggccg cggcatcatg ccgacggccc tcgcgatggt catcgatcag tgcttcgggg
     2041 acgccggctt gcatcgcgtc gaggcgaaca tcaggcccga gaacgcggcg agccgccggg
     2101 tggtagagaa gttgggcttc cggctcgagg gcacccgcga gcggtacatc cacatcgcgg
     2161 gcgcgtggtg tgaccacctg tcgtacgcgc tcacggtcga ggacgtgccc ggcggactgc
     2221 tcccgcggtg gctcgcggcg ggcggcggcg cccgattgag cagcgccgac acgacctgaa
     2281 gtcgacatat tccccgacac accaccgatg tgctcggcac atccgtcact cgtgtctagc
     2341 gtgacgaagt gtgagcagcc tgatcgtcct tggaatcgtg gggatttgga tcctggtgct
     2401 cgtcccgatg tggctcaatc ggcacgacgc cgatagcgcg agccgctcga tggacacctt
     2461 ttcgacggca atgcgggtgc tctcgcgccg cggaccgtct cgcggccggt tcgcccgttc
     2521 cgcccgcgcc gatcgcatcg ccgacggtca atacctcgtg atgccacgcg acaatagggg
     2581 agtcaccgtg gacaaccaga ccgacacatc caccggccag cgactgccga gacttcgggt
     2641 gccgctgcac ctgccgtcac gccgacggtc gtacgcgacc agcggccggg cccaggtgat
     2701 cgcccgccga cgccgcatcg cggtgggctt cgcagtcctc gtgatcggac tcgcgctttc
     2761 cgccgagttc gcgcacacct cgtggtggtt cgaactcggc gcctgcctgg cgctcgctgg
     2821 ctacctcatc cacctgcgca ccgaagccat caggacggtc gagatgcgcc gccgccggct
     2881 cgctcgcgta gcacgagcag ctgctcccta cccaacagcg cggaccgcgg ggggcgagca
     2941 ctactacgcg gctcgcgtcc ggcacgacgc tgacattgag ccggccgcga ctgacgcggt
     3001 cagcgccgcg ggagcggtca gtgcctcggg aacagaagac ggatcgcgat gggaacccat
     3061 cgccgtcccg ctgccgactt atgtgtcgaa gccggttgtg tcgcggccgg ccgttgcggc
     3121 gccgagggcg gacgttgagt ctggtcccac gattgacctc acccagccag gcaagtggat
     3181 cgattcgcag gtcgacgtcc ggtacctgct gcttgatgac gacgttgctg aggtcgacga
     3241 gctcgacgtg attctggccc ggcggcggg
//
LOCUS       DATCBJ010000477         2354 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_91998, whole genome shotgun sequence.
ACCESSION   DATCBJ010000477 DATCBJ010000000
VERSION     DATCBJ010000477.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2354)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2354)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2354
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_91998"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            193..>2354
                     /gene="lon"
                     /locus_tag="VII50_09685"
     CDS             193..>2354
                     /gene="lon"
                     /locus_tag="VII50_09685"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: protein motif:HMM:TIGR00763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HEY5051163.1"
                     /translation="MPNNSSNSAAHEVVELPVLWLDDEVILPGMVAPIALDAGTQAAV
                     DAARNAADSRLLIVPRNEGAYASHGVLAVIEHVGRLPQGGQAVVVRGVQRARIGAGVS
                     GAGAALWVQATLVEDSESTPRTAELAADYRALVISILQLRGAYQVIDTVQRVNDAATL
                     SDLAGYASYLDTEQKLWLLETADVDVRLEKLSEWARTQLAELEVSGRIRDEVREGMEK
                     TQREFLLRQQLAAIRKELGEDEPDGAEDYRARVEKAELPEKVQEAALREVGKLERGSD
                     QSPEAGWIRTWLDTVLELPWNTRTQDSTDITAARGILDADHEGLDDVKDRIVEHLAIR
                     TRRAERGLELVGGRGSGAVLALVGPPGVGKTSLGESVARALDRKFVRVSLGGVRDEAE
                     IRGHRRTYVGALPGRIVRAIREAGSMNPVVLLDEVDKIGSDYRGDPASALLEVLDPAQ
                     NHTFRDHYLELELDLSDVLFLATANVLDTIPDALLDRMEVVRLDGYTEDEKVSIARNH
                     LLPRQLSRAALDATEVDIDDDALRLIAGEHTREAGVRQLERAIARVLRKAATRLASAP
                     ENVPVHVTAQTLTEFLGRPRFTPESAERTAVPGVATGLAVTGAGGDVLFIEATAMVGE
                     TALTLTGQLGDVMKESAHIALSYLRSHGSQLGIDTSAFEQAIHLHVPAGAVPKDGPSA
                     GVTMVTALASLVTGRPVRDDVGMTGEVTLNGRVLPIGGV"
BASE COUNT          391 a          752 c          822 g          389 t
ORIGIN      
        1 gcgcttggcg ccccggccca gttgccgggg ccgggcgctg gagcgggcac gacgaccccg
       61 aaatcagccg atccgggggt tgggaacctc gacatgacgg gcgatcttgc caccacgctg
      121 tgggcgaacg cctggccggg aagcatcgcg gtcccccgtg agttgagcgt aggaaactca
      181 aggaggcggc agatgccgaa caattcttca aattcagcgg cccacgaggt cgtcgaactc
      241 cccgtgctct ggttggacga cgaggtcatc ctgcccggca tggtggcccc gatcgccctc
      301 gacgcaggga cgcaggcagc cgtcgacgcc gcgaggaatg cagccgattc gcggctgctc
      361 atcgtccccc gcaatgaggg ggcgtacgcg agccacggcg ttctcgccgt catcgagcat
      421 gttggccggc tgccgcaggg tggccaggct gtcgtcgtcc gtggggtgca gcgcgcaagg
      481 atcggcgcgg gcgtctctgg cgccggcgcc gcactgtggg tgcaggccac gctggtcgag
      541 gattcagagt cgacgccgcg aaccgctgag ctcgccgccg actatcgcgc gctcgtgatc
      601 tcgatcctgc agttgcgcgg cgcctaccaa gtgatcgaca cggtccagcg ggtcaacgat
      661 gccgcgacgc tgtcagatct cgccggctac gcgtcctatc tcgacaccga gcagaagctg
      721 tggctgctcg agacggctga cgtcgacgtc cggctggaaa agctctctga gtgggcacgg
      781 acgcagcttg ccgaactcga ggtcagcggc cggatccgcg acgaggttcg cgaaggcatg
      841 gagaagacgc agcgtgagtt cttgctgcgc cagcagcttg ccgcgatccg taaggaactg
      901 ggcgaggacg agcccgacgg cgcggaggac taccgggcac gggtcgagaa ggccgagttg
      961 ccggagaagg ttcaagaggc cgcgctgcgt gaggtcggta agctcgaacg tggcagcgac
     1021 cagagccccg aagcaggctg gatccgcacc tggctcgaca ccgtgctgga gctgccgtgg
     1081 aacacccgca cgcaggacag cactgacatc actgctgccc gtggaatcct cgacgccgac
     1141 cacgaaggct tggacgacgt gaaggaccgg atcgtcgaac acctcgcgat caggacccgg
     1201 cgtgccgagc gtggtctcga gctcgtcgga ggtcgtggaa gcggtgccgt gttggccctc
     1261 gtcgggcccc ccggcgttgg caagacgtcg ctcggcgaat ccgtcgctcg tgcgcttgac
     1321 cgcaagttcg tgcgtgtctc acttggtggc gtgcgcgacg aagcggagat tcgcgggcac
     1381 cgtcgtacgt acgtgggcgc gctgcccggt cgcatcgtgc gcgcgattcg cgaggccggt
     1441 tcgatgaacc cggtcgtgct gctcgacgag gtcgacaaga tcgggtcgga ctatcgcggt
     1501 gatcccgcga gtgcgctgct cgaagtgctt gaccccgcgc agaatcacac gttccgcgat
     1561 cactacctcg agctcgagct cgacctatcg gatgtgctct tcctcgcgac cgcgaacgtg
     1621 ttggacacca tccccgatgc gttgctcgac cgcatggagg ttgtgcggct cgacggttac
     1681 accgaggacg agaaggtctc gatcgcgcgc aaccacctgc tgccacggca attgtcgcgt
     1741 gcggctcttg acgcgactga ggtcgacatc gacgacgatg cattgcggct gattgccggt
     1801 gagcacactc gcgaagctgg cgtgcgccag ctcgagcggg cgatcgcgcg agtgctgcgc
     1861 aaggcggcga cccgactagc gtcggctccg gagaacgtgc cggtgcacgt cactgcacag
     1921 acactcacgg agtttcttgg gcgaccgagg ttcacgcccg agagtgccga acgtaccgca
     1981 gtccccggtg ttgcgaccgg tcttgcagtg accggtgccg gtggcgacgt gctctttatc
     2041 gaggccacgg cgatggtggg cgagaccgcg cttactttga ccggtcaact cggtgacgtg
     2101 atgaaggagt ccgcacacat cgcgctgtcg tacctgcgct cgcatggttc gcagctcggc
     2161 atcgacacgt cggcgttcga gcaggcaatt cacctgcacg tgcccgcggg tgccgtgcct
     2221 aaggacggcc catcggccgg cgtcacgatg gtcacggccc tggcgtcgct cgtcaccggt
     2281 cgtcccgtgc gcgacgacgt cggcatgaca ggcgaggtca cgctcaacgg ccgggtgttg
     2341 ccgatcggcg gcgt
//
LOCUS       DATCBJ010000478         3001 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_47965, whole genome shotgun sequence.
ACCESSION   DATCBJ010000478 DATCBJ010000000
VERSION     DATCBJ010000478.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 3001)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3001)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3001
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_47965"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            1..135
                     /locus_tag="VII50_09690"
                     /pseudo
     CDS             1..135
                     /locus_tag="VII50_09690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000387528.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0042255 - ribosome assembly [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="uL22 family ribosomal protein"
     gene            633..1250
                     /gene="rpsC"
                     /locus_tag="VII50_09695"
                     /pseudo
     CDS             633..1250
                     /gene="rpsC"
                     /locus_tag="VII50_09695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010986347.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
     gene            1640..2062
                     /gene="rplP"
                     /locus_tag="VII50_09700"
     CDS             1640..2062
                     /gene="rplP"
                     /locus_tag="VII50_09700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HEY5051164.1"
                     /translation="MLVPRRVKHRKQHHPGRTGAATGGTRIAFGEFGIQALEPAYVTN
                     RQIESARIAMTRHIKRGGKVWINIFPDRPITKKPAETRMGSGKGSPEWWIANVKPGRV
                     MFELAGVPEPIAREAMRRAMHKLPMKCRFVRREIGEAS"
     gene            2059..2283
                     /gene="rpmC"
                     /locus_tag="VII50_09705"
     CDS             2059..2283
                     /gene="rpmC"
                     /locus_tag="VII50_09705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HEY5051165.1"
                     /translation="MTTANDLRTLENDELVNKLHEAKDELFKLRFQYASGQLESHGRL
                     NSVRKEIARIYTVMREIELGITIKNDEDVA"
     gene            2280..2582
                     /gene="rpsQ"
                     /locus_tag="VII50_09710"
     CDS             2280..2582
                     /gene="rpsQ"
                     /locus_tag="VII50_09710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011727674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HEY5051166.1"
                     /translation="MSDKAVETTSKAGDADEKRGFRKTREGLVISDKMEKTVVVAVED
                     RVKHPLYGKVLRRTSKLHAHDEASGAAIGDRVLLMETRPLSATKRWRVVEILEKAK"
     gene            2614..2982
                     /gene="rplN"
                     /locus_tag="VII50_09715"
     CDS             2614..2982
                     /gene="rplN"
                     /locus_tag="VII50_09715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007073026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HEY5051167.1"
                     /translation="MIQQESRLRVADNTGAKEILCIRVLGGSGRRYARIGDVIVATVK
                     DALPGAGVKKGDVVKAVVVRTVKERRRPDGSYIKFDENAAVLIKDGGDPRGTRIFGPV
                     GRELREKKFMRIISLAPEVL"
BASE COUNT          586 a          920 c         1053 g          442 t
ORIGIN      
        1 cgcgttgacg tcgaagacct cgtggtgtcg gcggcctacg tcgacgaagg tccgacgatg
       61 aagcggttcc ggccgcgtgc gcagggtcgc gcctatcgga tccgcaagcg gacgagccac
      121 atcacgatcg aggtcgagtc gtggtcggcc gagatcgaga cgctcgccac caagcgttcg
      181 gttcccaagc gggtcaagac gccgatcgtg cgggttgccc cgacgcccag ggtgcgggcg
      241 gttcaggagc cagccccggt cgtggctgcg aagccggcga agaagacagc ggcgaagaag
      301 acggctgccg ccgtcgaggc agcggacgtg gagacgccgg acatccagga cgtcgagccg
      361 accgacgctg tcgaggccgt cgaggtcaag ccggcgaaga aggtcgcagc caagaagacc
      421 gcggcggccc acgaccacga tcacgaggct gaggctaagc cggccaagaa ggtcgccgcc
      481 aagaagacgg cgccggcagc ggttgcacag ccagcggtga ctgtaacgcc ggcgaagaag
      541 accgccgcca agaaagctgc gcccgcgaag gcgagcgacg catcggatgc cgacacctcg
      601 gaatccgacg atggaaagga agggacccgc tagtgggcca gaaggtcaac ccgcacgggt
      661 tccggctcgg catcaccacc gaattcaaga gccggtggta cgccgacaag ctgtaccggg
      721 attacgtgaa agaagacgtc gccattcgcc gcatgatggc gaagggcatg gaacgcgcag
      781 gcatctcgcg ggtcgagatc gagcgcaccc gtgatcgggt tcgcgttgac atccacaccg
      841 cacgtccagg cattgtgatc ggacgccgtg gtgcggaggc cgaccggatc cgtggcgacc
      901 tcgagaagct gacgagcaag caggtccagc tgaacatcct cgaggtgaag aacccggaga
      961 tcgacgcgca gctcgtcgcc cagggtgttg ccgagcagct cacccaacgt gtgtcgttcc
     1021 gtcgtgcgat gcgcaaagcc atgcagagcg cgatgaagtc gggcgccaag ggaatccggg
     1081 ttcaggtgtc cggtcgcctc ggcggcgcgg agatgagccg gaccgagttc taccgcgaag
     1141 gtcgcgttcc gctgcacacc ttgcggtccg acatcgacta cggcctctac gaggcgcgca
     1201 ccacgttcgg ccgcatcggg gtcaaggtct ggatctacaa gggtgacgtc gtcgtcgggc
     1261 gccccgagta cgagcccggc gctgtccccg gccgcatccc gccccgcgct cccgtgggcc
     1321 gtccggtccg tgcgccgcgc gcagctcgtc cggttcgcga ggcgatcccg gctgctgtcg
     1381 agaccgtcga ggtgcttgat gtcgagcccg cggacgtcga gccagcgatc gtcgagtcgg
     1441 acgtggtcga ggtagtcgcg gcggagccgt cagcggtcga ggtagtcgcg gctgagccgg
     1501 tagctgtcga agcgcctgcc gccgacgcac cggtggccgc tgtaccggtg gccgctgtac
     1561 cggtggccgc tgcagccgag gccgacgcac ccgtcgtccc ggctgtcgaa gtgcctgaac
     1621 cggaagagga gagctagtca tgctggttcc tcgcagggtc aagcaccgca agcagcatca
     1681 cccgggccgc accggcgcgg cgaccggtgg cacgcgtatc gccttcggcg aattcggcat
     1741 ccaggctttg gagccggcgt acgtcaccaa ccgtcagatc gagtctgcgc gtatcgcgat
     1801 gacccgccat atcaagcgcg gtggcaaggt ctggatcaac atctttcccg accggcccat
     1861 caccaaaaag cccgccgaga cccgtatggg ttccggtaaa gggtcaccgg agtggtggat
     1921 cgcgaacgtg aaacccggcc gggtcatgtt cgaactggct ggggttcccg agccgatcgc
     1981 ccgggaggcg atgcggcgcg ctatgcacaa gttgccgatg aagtgccggt ttgtccggcg
     2041 cgagatcggg gaggcgtcat gaccaccgca aatgacctgc gcacgctcga gaacgacgag
     2101 ctggttaaca agctgcacga ggcgaaggac gagctgttca agctgcggtt ccagtacgcg
     2161 tcgggccagt tggaaagcca cggtcggttg aactcggtac gcaaggaaat cgctcgcatc
     2221 tacacggtca tgcgtgagat cgagctcggc atcaccatca agaacgacga ggatgtggca
     2281 tgagcgacaa ggccgtcgaa acgacttcga aggcgggcga cgccgacgag aagcgcggct
     2341 tccgcaagac ccgcgaggga ctcgtgatca gcgacaagat ggagaagacc gtcgtggtcg
     2401 cggtcgagga ccgggtcaag caccctctct acggcaaggt cttgcgccgc accagcaagc
     2461 tgcatgctca cgacgaggcc agtggcgctg cgatcggcga ccgggtgctg ctgatggaga
     2521 cgcgcccgct gtctgccacg aagcgctggc gcgtcgtcga gatcctcgaa aaagccaagt
     2581 aaaccccaag caatttaagg accgggagtc acagtgattc agcaggagtc gcgactgcgt
     2641 gtcgccgaca acacgggtgc caaggagatc ttgtgcatcc gtgtgctcgg gggctcgggc
     2701 cggcgctacg cgcgaatcgg cgacgtcatc gtggccaccg tcaaggacgc gttgccgggt
     2761 gcgggtgtga agaagggcga cgtcgtgaaa gcggtcgtcg tgcgtaccgt gaaagagcgg
     2821 cggcgtcccg acggttcgta catcaagttt gacgagaatg ccgcggtcct gatcaaggac
     2881 ggcggcgacc cgcgtggcac gcgcatcttc ggcccggtcg gccgcgagtt gcgcgagaag
     2941 aagttcatgc ggatcatctc gctggctccg gaggtgctgt aagtcatggc aggtctgaag
     3001 g
//
LOCUS       DATCBJ010000479         2438 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_26931, whole genome shotgun sequence.
ACCESSION   DATCBJ010000479 DATCBJ010000000
VERSION     DATCBJ010000479.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2438)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2438)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2438
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_26931"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1166
                     /locus_tag="VII50_09720"
     CDS             <1..1166
                     /locus_tag="VII50_09720"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HEY5051168.1"
                     /translation="DRTAMRRRAEAAFARLGVALDSNRLARGLSVADQQIVEIAKAIS
                     FNARVIVMDEPTAALSSVEVDRLFAVVRALRADNTAVLFISHRLEEVFAICQRVTIMR
                     DGRFVRTAPVEELTIDDIIRSMVGRDLDTLFPKTETAPGAEILDVSRLTREGVFTDVS
                     FTVRRGEIVGLSGLVGAGRSEVARAIFGIDRYDAGTVTVGGKLLPNGSPIAAMDAGIA
                     FVPEDRRLQGLVMDMAIDHNVALASLGRLRRRGLISRARERDLAADWAVRLRLKYARL
                     KNPVSTLSGGNQQKVVLSKWLARNPTLLIVDEPTRGIDVGTKAEVHRLLDALVAKGVA
                     VLMISSELPEILGMADRILVLREGRLVAEMPRDAATEASIMRAATGQNVGSAA"
     gene            1163..2191
                     /locus_tag="VII50_09725"
     CDS             1163..2191
                     /locus_tag="VII50_09725"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY5051169.1"
                     /translation="MTSTTAPTLNRISRSGSLLRARELGIAIVIVIVFGVTTVHNHAF
                     ASEHSVQQLLAGAALVALLGVGETLVIVTRNVDLSVGSVLGLSAYLVGDLFVHYPHMP
                     VIFGFVVGTAIGAGIGAINGLITTVTRVPSLVVTLAALYIVRGVDAIIVNGKQTDPTS
                     IPRSFQAVGYETIFGVPWLAVVVAVIIALVGYAMRSFRSSRDLYAIGSNPDAAALAGL
                     PVGRRVFTAFVVSGALAGLGGALFLAEFATINATGGTGYELLVISAVVVGGVAIFGGS
                     GTVLGAALGALLLNTINQALVASRISSFWNQAIAGALLLGAIAFDRWLGLRVSRRLQS
                     AKGQRHVV"
     gene            2181..>2438
                     /locus_tag="VII50_09730"
     CDS             2181..>2438
                     /locus_tag="VII50_09730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5051170.1"
                     /translation="MSSNSTLSQAPVAEPPAKNSASWRGLVRWESGLVLVLLASILFG
                     ASESPHFLTATDVFFIGLNMGEIAIMALPLTLIVMTGEIDLS"
BASE COUNT          366 a          802 c          788 g          482 t
ORIGIN      
        1 tcgaccggac cgcgatgcgt cgccgagccg aggctgcgtt tgcccgcctc ggcgttgcgc
       61 tcgattccaa ccggctcgcg cgtggcctct cggtcgccga tcagcagatc gtcgagatcg
      121 ccaaggccat ctccttcaat gctcgggtca tcgtgatgga cgagcccacc gcggccctca
      181 gctccgtcga ggttgaccgg ctcttcgccg tagtccgcgc gttgcgggcc gacaacaccg
      241 ccgtcctctt catctcgcat cggctcgaag aggtctttgc gatctgtcag cgggtcacga
      301 tcatgcgcga cggcaggttc gtgcggaccg caccggtcga ggaactcacc atcgacgaca
      361 tcatccggtc gatggtcggt cgcgacctcg acacactgtt cccgaagacg gaaacagcgc
      421 ccggagcaga gatcctcgac gtctcgcggc tgactcggga aggcgtcttc accgacgtgt
      481 cgttcacggt tcgtcgtggc gagatcgtcg ggctgtccgg gcttgtcggg gcgggtcgca
      541 gcgaggtcgc acgtgcgatc ttcggcatcg accgctatga cgccggcacc gtgactgtgg
      601 gcggcaagtt gctccccaac ggctcgccga tcgcggcgat ggacgccggc atcgcctttg
      661 tccccgagga tcgccgcctg caaggcctcg tcatggacat ggccattgac cacaacgtcg
      721 ccctggcatc acttggtcga ctccgccggc gcggtctgat ctcgcgggcg cgagagcgcg
      781 accttgcggc ggattgggcg gtgagactgc ggctgaagta cgcgcgcctg aagaacccgg
      841 tctcgacgct gtcgggtggc aaccagcaaa aggtcgttct cagcaagtgg ttggcgcgca
      901 atccgacgct gctcatcgtc gacgaaccga ctcgcggcat cgacgtcgga accaaggccg
      961 aggtgcaccg gctgctcgat gcccttgtcg caaaaggtgt cgcggttttg atgatctcgt
     1021 ccgagctacc cgagatcctc ggaatggccg accggattct ggtcctgcga gagggtcggc
     1081 tggtcgccga gatgccacgc gacgcggcga ctgaggcgtc gatcatgcga gccgcaactg
     1141 gccagaacgt cgggtcggca gcatgacctc gacgacggcg ccgacactga accggatcag
     1201 ccggagcggc tcgttgctgc gagcacgtga actcggcatt gcgatcgtca tcgtcatcgt
     1261 gttcggggtc acgacggtgc acaatcacgc gttcgccagc gaacacagcg ttcagcagct
     1321 cctcgccggc gcagcgttgg ttgctctgct cggggtcggc gagacgctcg tgatcgtcac
     1381 ccgcaacgtc gacctctccg tcgggtcggt gctcgggctc tccgcgtacc tcgtggggga
     1441 tctgttcgtc cactacccgc acatgccggt gattttcggg ttcgtcgtgg gcaccgcgat
     1501 cggcgccggt atcggcgcga tcaatggcct gattacaacg gtgacccggg tgccaagtct
     1561 cgtggtcacc ctcgccgcgc tctacatcgt tcgcggcgtc gacgcgatca tcgtcaacgg
     1621 caagcagacc gatccgacga gcatcccgcg ctcgtttcag gcggtcggct acgagaccat
     1681 tttcggcgtc ccgtggctcg cggttgtggt tgcggtgatc atcgctctcg ttggctacgc
     1741 catgagatcg ttccggtcca gtcgcgactt gtacgcgatc ggctccaacc cggatgcggc
     1801 ggccctcgca ggtcttccag tcggacgacg cgtcttcacc gcgttcgttg tcagcggggc
     1861 gctcgccgga cttggtgggg cgttattcct tgcagagttc gccaccatca acgcgaccgg
     1921 cggcaccggc tatgagctgc tcgtgatttc cgctgtcgtg gtcggtggcg tcgcgatctt
     1981 cggcggtagc ggcaccgtgc ttggcgccgc gcttggtgcg ctgttgctca acacgatcaa
     2041 ccaggcgctc gtcgcgtcga gaatctcgtc gttttggaac caggcgattg ccggcgccct
     2101 gctgctcggc gccatcgcct tcgaccgttg gctcggcctg cgggtaagcc gtcgcctgca
     2161 atcggcgaag ggccaacgtc atgtcgtcta actccacgtt gagccaggcg ccggtcgccg
     2221 aaccacccgc gaagaactcc gcttcgtggc gaggactcgt tcggtgggag agcggtctcg
     2281 tgctggtgct gctcgccagc atcctcttcg gggcctccga gtctccgcac ttcctgacgg
     2341 ccaccgacgt gttcttcatc gggctgaaca tgggcgagat cgccattatg gcgctaccgc
     2401 tcacgttgat cgtcatgacc ggcgagatcg acctttct
//
LOCUS       DATCBJ010000480         6172 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_373997, whole genome shotgun sequence.
ACCESSION   DATCBJ010000480 DATCBJ010000000
VERSION     DATCBJ010000480.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 6172)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6172)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6172
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_373997"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2071
                     /locus_tag="VII50_09735"
     CDS             <1..2071
                     /locus_tag="VII50_09735"
                     /inference="COORDINATES: protein motif:HMM:NF012906.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycoside hydrolase family 2 protein"
                     /protein_id="HEY5051171.1"
                     /translation="ALAITFAAGLTAAEHASAALGPRPHVNAHPFNALRKMACNFGWD
                     WGPDLVTAGIWRPLLLEIWHDARIDTVRPLVELDDTTGLLHAHIDIERSADYDCALQL
                     DVRVGGSWASRTLAPDETSAVVDVAVHDVNVWWPHGYGEQSLYPVTVTLSGDHGALAT
                     WECRVGFRTIRLDTGVDEHGSAFAFSVNGVPVFTRGVNWIPDDIFPSRITHERYAERL
                     TQARDANVNFIRVWGGGIYESEDFYDECDELGLLVWQDFLFVCAAYAEEEPLRSEVVA
                     EAREAVTRLSAHPSLALWNGNNENIWGHEDWGWKEHLGDRTWGWGYYTDVLPSIVAEL
                     DPTRPYSPGTPYSFTDGLPPNDPTHGTMHIWDVWNELDYTAYRRYVPRFVAEFGFQGP
                     PTWATLTDAIHDDPLSSESPGILSHQKADDGNGKLARGLETHFPQPDSFDDWHWATSL
                     NQARGVALGIEHFRSWSPVCMGTVVWQLNDIWPAISWAAIDGAGRRKPLWYALRRSFS
                     DRLLTMQPRDGGLTLIAVNDSGQAWREDVTLTRRTTEGVVLDTTVVSLDVAPRCTFAV
                     PISAELAAAGAKAGELLLAEARAHRAWWHGCEDIDAGLPAPDLTATAERLPGGYRLTV
                     TAHALVRDLTVLADRLSPDACVDDMLVTLLPGESVVFTITTSAQLSLEQLTDPLVLRS
                     ANQLVTG"
     gene            complement(2053..3888)
                     /locus_tag="VII50_09740"
     CDS             complement(2053..3888)
                     /locus_tag="VII50_09740"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY5051172.1"
                     /translation="MNLSASGARSRSAATASHLVDRRYDPTHPARTALRLYSGQRRRL
                     GLAAAAFAGKQSPVWIAPLLTANIIDAVVSRKPVSVIWENAAVMAGLIVVNAPLTWAY
                     VRWLSLALRTVEVESRTALCERLQQLSIGYHKKMSAGVLQTKVIRDVETIIDTSRQTF
                     DSGMAATTTLIGALVLTTWRVPEFLPVFLIAVPAAATLIHGMRRRMSDHNKAFRRQVD
                     QLSARVSEMTNLIQVTRAHALERDELNRIDTTLHDVREKGVRLDVLNGRFNALAWILF
                     QLLSVGCLVVAAWGARTGALSVTPGDIVLLSTYFATLTGSVTMLLNVVPLVSKGIESV
                     RSMAEVLTEPDLEVNEGKQIMERVEGRVTFDHVSLTYPDAAEQALVDICLDVQAGETV
                     ALVGPSGSGKSSMLNLVIGFLTPTQGRLLLDGGDRRDLDLRGYRRFLGVVPQETLLFD
                     GSVRDNVTYGQEDLSDADVRAALEAANAWDFVETMGGIDTVIGQRGSRLSGGQRQRLA
                     IARALIRNPRVLILDEATSALDPASETLVQDALARLMHGRTSFVVAHRLSTVQRADRI
                     VVLRAGRIVEIGTHDELIAAGGAYVELQGLGRKQGRSGSGQPVTS"
     gene            3909..4886
                     /locus_tag="VII50_09745"
     CDS             3909..4886
                     /locus_tag="VII50_09745"
                     /inference="COORDINATES: protein motif:HMM:NF012377.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
                     /protein_id="HEY5051173.1"
                     /translation="MPRSGFNFQWLYSKRAAPRGPDQKALDAIAAWGFDFVRLPTDYR
                     LWSVDDDSHRPDEAVLSLIDEVLVACRQRGIHLSLNMHRAPGYVITGWETEPYNLWAD
                     LPAQDAFVASWERFAQRYVEVPGEALSFDLVNEPPALGLRGFTRDAHEAVIRRTVAAI
                     RAISRDRPLVIDGLDGGNLAMPELADLGLTQSVRGYQPMRVSHYEAPWWPGHVGMPEP
                     AYPCEYDGRWWDRAGLREFYQPWRDLEAQGVPVHVGEFGCYERTPDDVAQAWFTDLFG
                     VFNEFGWGFGLWEFEGPFGIVGHSRPGAVFEAKDGFLVDRRLLDLMMNR"
     gene            complement(4893..5444)
                     /locus_tag="VII50_09750"
     CDS             complement(4893..5444)
                     /locus_tag="VII50_09750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5051174.1"
                     /translation="MSEPTDPAYPPLPPAPGYAGYGAMPPAEYGQPVLVGPAPSSVMN
                     AVRLMFVAAALGVVSLIVALSTKSTLRSAIVKKNPDFDSQKINTAVNVAIVAGVVFGI
                     VFITLFVLLALQVRTGKNWARVVTWVITGLGIVSALASLGQTVAPASRVVGLVSGILD
                     VAIVVLLLQKPSNAFFKPARSGF"
     gene            complement(5453..>6172)
                     /locus_tag="VII50_09755"
     CDS             complement(5453..>6172)
                     /locus_tag="VII50_09755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5051175.1"
                     /translation="WSLLLLVILTPGLSALLTWLNDHDWAKADPSQRANITDDPTSFI
                     LGTGFAFGQLTICVLGVLVMAGEYSTGSIRSSLLAVPKRTPMLAAKCAVFAFLIFVFG
                     ELVVFPSFLIGAKLLHHHAPVSLSDPGVLRAVIGGGLYLAVLGLFALAIGALVRHTAG
                     AITGVIAFVLVLAPLAQLLPGSLGKHVHAYLPSEAGHLIGQAHQSKTDLLSPWQGFGV
                     FCLWTAVLLALAGWLLNRRDA"
BASE COUNT         1130 a         1947 c         2024 g         1071 t
ORIGIN      
        1 cgcgctcgcg attacgtttg cggccggctt gactgctgcg gagcacgcga gcgcggcgct
       61 cgggccgcgt ccgcacgtca acgctcaccc gttcaacgcg ctacgaaaga tggcctgcaa
      121 cttcggttgg gactggggcc ccgaccttgt gactgcagga atctggcgcc ccttactcct
      181 agagatctgg cacgacgcac gcatcgacac ggttcgccca ttagtcgaac tcgacgacac
      241 aaccggattg ttgcatgccc acatcgacat cgaacgcagc gccgactacg actgcgccct
      301 gcaactcgac gtccgggtgg gtgggtcgtg ggcttcgcgg acgcttgctc ccgacgagac
      361 ctcagccgtc gtcgacgttg cggtccatga tgtcaacgtt tggtggccgc atggttatgg
      421 agaacaatcg ctttacccgg tcaccgtgac gctgtccggt gaccatggag cgctggctac
      481 atgggaatgc cgggtgggtt tccggacgat ccgactcgac acgggcgtcg acgagcacgg
      541 tagcgcgttc gcgttctccg tcaacggcgt gccggtgttt acacgtggcg tgaactggat
      601 accggatgac atcttcccgt cgcggatcac ccacgagcga tatgcggagc gccttactca
      661 ggctcgcgat gcgaacgtca acttcatccg ggtctggggc ggggggatct acgagagcga
      721 ggatttctac gacgagtgcg acgagctcgg attgctggtg tggcaagact tcctgttcgt
      781 gtgcgcggcg tacgccgagg aggagccgtt gcgcagcgag gtcgtcgccg aggcacgcga
      841 ggcggtgacc aggctcagtg cgcatcccag cctggctctg tggaatggca acaacgagaa
      901 catctggggc cacgaggact gggggtggaa agagcacctc ggcgaccgga cgtggggttg
      961 gggctactac accgacgtcc tgccctcgat tgtcgccgag cttgacccga cgcgtccgta
     1021 cagccctggc acgccgtact cgttcaccga tggcctgcca cccaacgatc cgacccacgg
     1081 cacgatgcac atttgggatg tgtggaacga gctcgactac accgcctacc gacgctacgt
     1141 tcctcgcttc gttgcagagt tcggattcca gggccccccg acctgggcaa ccctcacgga
     1201 tgcgatccac gatgacccgc tcagctcgga gtcgccgggg atcttgtcgc accagaaggc
     1261 tgacgacggc aacggcaagc tcgcccgcgg tcttgagaca cacttcccgc agccagatag
     1321 cttcgacgac tggcactggg caacctcgct gaatcaggcg cgaggggttg ctctggggat
     1381 cgagcacttt cggtcgtggt cgcctgtttg catggggaca gttgtctggc agctcaacga
     1441 catctggccg gcgatctcgt gggctgcaat cgacggcgcc ggacgtcgca agccgctgtg
     1501 gtacgccttg cgccgcagct tcagtgatcg gttgcttacc atgcagccgc gcgacggcgg
     1561 cctcaccctg atcgccgtca acgattcggg gcaggcatgg cgcgaggacg tcacactgac
     1621 ccgccgtacg accgagggcg tcgtgctcga caccaccgtc gtgtccttgg acgtcgcacc
     1681 gcgatgcacc ttcgcggtgc caatatcggc cgaactagct gcagctggag cgaaggctgg
     1741 agagttgctg ctggccgaag cacgtgcaca ccgcgcgtgg tggcacgggt gcgaggacat
     1801 cgatgcaggc ttgcccgcgc ccgacctgac cgcgacggcc gagcggctgc cgggggggta
     1861 ccggctgacg gtcaccgccc acgcgttggt tcgcgatctc accgtcttgg cagaccggtt
     1921 gtccccagat gcatgcgtcg acgacatgct ggtgaccctg ttgccgggtg agtcggtggt
     1981 gttcacgatc acgacatcgg cgcagctgtc cctcgagcag ctaacagatc cgctggtctt
     2041 gcgttcggcg aatcagctcg taacgggttg accactaccg gagcggccct gcttgcgccc
     2101 gagaccctgc agttcgacgt aggcaccgcc ggccgcgatg agttcgtcat gcgtgccgat
     2161 ctcgacaatg cgtcccgccc gcagcaccac gattcggtcg gcgcgctgca ccgtcgacag
     2221 ccgatgagcc accacgaacg aggtacgacc gtgcatcagc cgagcgagtg catcttgaac
     2281 caacgtctca gacgccgggt cgagcgcgga ggtcgcctca tcgagtatca gcacgcgcgg
     2341 gttgcggatc aatgcacgtg cgatcgcgag ccgctgccgc tggccgccag acaagcgact
     2401 cccgcgctgg ccgatcactg tgtcgatgcc gcccatggtc tcgacgaaat cccatgcgtt
     2461 cgctgcctcc aacgccgcac gcacgtcagc gtcagacagg tcctcctgcc cgtaggtgac
     2521 attgtcgcga accgacccgt cgaagagcag cgtctcctgc gggacgacgc caagaaagcg
     2581 gcgatagcca cgcaggtcga gatctcgtcg gtcgccgccg tcgaggagca gccgcccctg
     2641 cgtcggcgtg agaaagccga tgacgaggtt cagcatgctc gacttgcccg atccagacgg
     2701 cccgaccagg gctacggtct cgccggcctg gacgtcgagg cagatgtcga ccaacgcctg
     2761 ctctgcggcg tccggataag taagggatac gtgatcgaac gtgacccggc cctcgacgcg
     2821 ttccataatc tgcttgccct cgttgacctc gaggtcgggc tcggtgagca cttctgccat
     2881 ggaccgcacc gattcgatcc ccttcgacac cagtggcacg acgttgagaa gcatcgtgac
     2941 ggacccagtg agggtggcga agtaggtgga cagcaacacg atgtcgcccg gcgtaacgga
     3001 cagcgcgccg gttcgcgcgc cccacgctgc gaccacgagg cagccgacgc tgaggagctg
     3061 gaacaagatc caggccaacg cgttgaagcg accgttcaga acatcgaggc ggacgccctt
     3121 ttcccggacg tcgtgcaatg tcgtgtcgat ccggttgagt tcgtcgcgct cgagggcatg
     3181 ggcgcgcgtc acctgaatca gattcgtcat ctcgctgact cgcgccgaga gttggtcaac
     3241 ctgccggcgg aatgccttgt tgtggtcgct catgcggcgg cgcatgccgt gaatcagggt
     3301 cgcggccgct gggaccgcga tcaggaagac gggcaagaac tccggcactc gccatgtggt
     3361 cagcaccagc gcgccgatca gtgtagtggt cgcagccatg cccgagtcga aggtctgacg
     3421 cgaggtgtcg atgatggttt cgacatcgcg aatgaccttc gtctggagca cgccggcgct
     3481 catcttcttg tggtatccga tcgacaactg ctgaaggcgc tcgcacagcg cggttctgga
     3541 ttcgacctca acggtgcgaa gcgcgaggct cagccatcgc acgtaagccc aggtcagtgg
     3601 agcgttgacg acgatcaggc cggccatcac cgccgcgttc tcccagatca ccgacaccgg
     3661 cttgcgcgag acgaccgcgt cgatgatgtt cgcggtgagc agcggcgcga tccagaccgg
     3721 cgactgcttg ccggcgaacg ccgcggcggc gagcccgagc cgtcggcgtt gccccgaata
     3781 caaccgcagc gcggttcggg cgggatgagt cgggtcgtac cggcgatcga ccagatgact
     3841 tgctgtcgcg gcactcctac ttctcgcccc tgaagcgctt aagttcacaa ccaggaaagg
     3901 tactcctcat gcctcgatcc ggcttcaatt ttcagtggct gtacagcaag agggccgcac
     3961 cgcgtggccc ggaccagaag gccctcgatg cgattgccgc gtggggattc gatttcgtcc
     4021 gactgccgac cgactaccgg ctatggagcg tggacgacga ttcgcaccgg cccgatgaag
     4081 ccgtgctctc gctcatcgac gaggtcctgg tcgcgtgccg tcagcgtggg atccacctgt
     4141 cgctcaacat gcatcgggca ccgggctacg tgatcacggg atgggagacc gagccgtaca
     4201 acctgtgggc cgatctgccc gctcaagatg cgttcgtcgc aagctgggag cgctttgccc
     4261 agcgctacgt cgaagtgccc ggcgaggcac tcagcttcga tctcgtgaac gagccgccgg
     4321 cacttggcct gcgcggtttt actcgggacg cgcacgaagc ggtaatccgt cgaacggtgg
     4381 cggcgatccg cgcaatctcg cgtgatcgtc cgctggtgat cgacgggctc gacggcggca
     4441 acctggccat gcccgaactc gctgatctcg gactcaccca aagcgtgcgt ggctaccagc
     4501 cgatgcgggt gtcgcactac gaggcaccgt ggtggccagg tcatgtcggc atgcccgagc
     4561 cggcgtaccc ctgtgagtac gacgggcgtt ggtgggatcg cgccggcctg cgggagttct
     4621 accagccgtg gcgtgacctc gaggcgcaag gcgtaccggt acacgttggg gagttcgggt
     4681 gctacgagcg gacgccggac gacgtagcgc aggcgtggtt caccgacctg ttcggcgtgt
     4741 tcaacgagtt cggctgggga ttcgggctgt gggaattcga gggaccgttt ggcatcgtcg
     4801 ggcactcgcg tccgggagct gtcttcgaag cgaaagacgg cttcctggtc gaccgccggt
     4861 tgctcgactt gatgatgaac cgttagaggc tctcagaatc cgctacgtgc gggcttgaag
     4921 aaggcgttgc tcggcttttg aagtagcagc acgacgattg ccacgtcgag gattccgctg
     4981 acgagtccga ccacccggct ggcgggcgcg acggtctggc cgagggacgc gagcgcgctg
     5041 acgatgccca acccggtgat gacccaggtg acgacccgcg cccagttctt gcccgtgcga
     5101 acctgtagcg cgagcaacac gaacagcgtg atgaagacga tgccgaacac cacgccggcc
     5161 acgatcgcca cgttcacggc ggtgttgatc ttctgcgagt cgaaatcggg attcttcttg
     5221 acgatagcgc tgcgcaacgt gctcttggtc gacagggcca cgatgagcga caccacgccg
     5281 agcgcagcgg cgacaaacat cagccgtacc gcgttcatca ctgaggaagg cgcgggcccg
     5341 accagaaccg gctgtccgta ctcggccggc ggcatcgctc cgtatcccgc gtagccagga
     5401 gctggtggca gcggcggata agcgggatca gtcggctcgg acatgtgcgc ccctaggcat
     5461 cgcgacggtt caacagccag cccgcgagcg cgagcaggac tgctgtccac aagcagaaga
     5521 caccaaaccc ctgccacggg gacagcaggt cagtcttgct ttgatgcgcc tgtccgatca
     5581 agtgcccggc ctctgacggg aggtacgcgt gcacgtgctt gccgaggctg cctggtagca
     5641 actgggcgag gggagcgaga acgagcacga acgcgatgac tcctgtaata gcgcccgcag
     5701 tgtggcgcac cagtgcgccg atcgcgagtg cgaatagccc gagcaccgcg aggtaaaggc
     5761 cgccaccgat taccgcccgc agcactcctg ggtcacttag tgagacgggc gcgtggtgat
     5821 gcagcagctt cgcgccaata agaaacgatg ggaacaccac cagttcgccg aacacgaaga
     5881 tgaggaaggc gaagacggca cacttggctg caagcatcgg cgttcgcttt ggcaccgcga
     5941 gcaggcttga tcgaatggat ccggtcgagt actcaccggc catgaccagc acgccgagca
     6001 cgcagattgt cagttggccg aacgcgaaac cagtgccgag gatgaaactg gttgggtcat
     6061 cggtgatgtt cgcgcgctgc gagggatcgg ccttcgccca gtcgtgatcg ttgagccagg
     6121 tgagcagtgc gctaagaccc ggcgtgagaa tgacgagcaa aagcagcgac ca
//
LOCUS       DATCBJ010000481         5568 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_303907, whole genome shotgun sequence.
ACCESSION   DATCBJ010000481 DATCBJ010000000
VERSION     DATCBJ010000481.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 5568)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5568)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5568
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_303907"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..328)
                     /locus_tag="VII50_09760"
     CDS             complement(<1..328)
                     /locus_tag="VII50_09760"
                     /inference="COORDINATES: protein motif:HMM:NF021214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase assembly protein"
                     /protein_id="HEY5051176.1"
                     /translation="MHGRGLRRHVRAIAKAGRPVRPWIGQALIFWAGLVVLLLAVVSP
                     IDYWSDTYLSAHVVQHILLAFVAPPLIVLGAPWVPLLRGLPRPVARAYGRLLRYTRAR
                     RPVVDRP"
     gene            444..1106
                     /locus_tag="VII50_09765"
     CDS             444..1106
                     /locus_tag="VII50_09765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5051177.1"
                     /translation="MTDPTTPTQTPTQQPSTSTQPWLAWILRHRKLSIAGAVVIVLAL
                     VAVVDWPHRATAGQLRSDFAQYATQVRGDVQSCSVEVEETLSAYNQITAGVVRDRQTA
                     EAIASQTALDCTPMGNSRIDDLGTLQPPSSLANFALGASTQQLYVWCFPTAVDVAQDI
                     EHLLSSPGDPATLADVKTRLAQINSAGASAQTVFNKAAGSLNTPPVDFGLDQVRASVL
                     VG"
     gene            complement(1238..1459)
                     /locus_tag="VII50_09770"
     CDS             complement(1238..1459)
                     /locus_tag="VII50_09770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5051178.1"
                     /translation="MPLFAIYCIVTAYWLETLLARSLRVRWVLSPEGPNRDDRIQIAF
                     AGSALGAKAVVVFVSVVAIVLFLLFSVLN"
     gene            complement(1885..3852)
                     /locus_tag="VII50_09775"
     CDS             complement(1885..3852)
                     /locus_tag="VII50_09775"
                     /inference="COORDINATES: protein motif:HMM:NF012343.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HEY5051179.1"
                     /translation="MAMDTTAPPAVDTAGNPGGPRASRAVRPWWLGPNLLLAFVGAAA
                     GYAIGHMVGNWIAHTYQQVQGEGQNNHALVLGYLFATFGWLAGIGALNYPFQKLFGYA
                     PASEAEGAHERKPTIRSYFSYTLDHKVVGVQYLVAMLVYFFVAGLFAMAIRSELLSPS
                     RHILQPGTYLEVVGEHGTMMMMMMTSVILGPFGNYLVPLMIGSRRMAYPRLEALSFWI
                     TPAAFIVLLSGIIVGGFPTGWTGYAPLSIEAGQGQDAYLVAFGLMAFSMIVGGFNIIC
                     TVINYRAPGMTWSRVPIFVWSMLVTAMLMVLAAPVLVAATYMSLMDRTVQTALFDASH
                     GGSSYLWENLFWFFGHPEVYILALPGFGIALELLTVFARKPLFGYRTAVAGMVGVGTL
                     SFFVWQHHLFDSGMNPDMRPVVMLTTELISIPTGIIYLVAVGTLWRARLRLTVPMLFV
                     LGMLFNFLIGGISGVFLSDVPTDAVAHGSFFVMAHFHYTIMGGLIFAFFGGVYYWLPK
                     MTGRQLNQKLGRIHFWALFVFFNSTFLPLFAIGELGMPRRVFEYEKALQGLNDWVSIS
                     AYLIGLSMLVFVYNFVKTMFVDPRPTEPVGNPWGSRGLEWMTSTPPVAYNFAHVPTVV
                     SSPYEYGTGAGPMAYIGAAPPGIPTQPEEVK"
     gene            complement(3866..4840)
                     /locus_tag="VII50_09780"
     CDS             complement(3866..4840)
                     /locus_tag="VII50_09780"
                     /inference="COORDINATES: protein motif:HMM:NF012344.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit II"
                     /protein_id="HEY5051180.1"
                     /translation="MTQPASSEPTVRPERTDRQHILRIAVIWIVLSVIGMLLVYFVWG
                     PHMPPGKDSDAARVQQFDNRVLGTIAVPVFVFVWVFVGYVLTNWRVKSSTPVDEVGDG
                     PPMKGHRGFQAAWLGITSVTVISLFVFGTVELDTNAGAGTGSGQVPIFSPTGYSSNVS
                     QTKLLVVQVIGQQWRWTFRYPQYGGLETAQMVVPAGQEVQYSVTSLDVIHSFWAVNLG
                     VKADANPGVNNVAFATADKIGSVNIRCDELCGLWHGAMTTTGKVVSPADFKTWVTNAS
                     ATYQQLIPFLPPYADIYLPQSDGGYYDPGQDPLPSGPPPGPASPSAIP"
     gene            complement(4840..5514)
                     /locus_tag="VII50_09785"
     CDS             complement(4840..5514)
                     /locus_tag="VII50_09785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5051181.1"
                     /translation="MTRPMRATAVGVVFAVFVAGVTMLFLYFLHYPPTVAAAATTGSS
                     GQEEIHLQTVPALGYGPHPTYVSYLADINGKWVHTTMFELKANTLVHFTIDQYDTGST
                     LRNSFMDQVQGTVDGTEVLNGGSPVRIINDNTDNGIGHTFAVPTLGISVPMPGIPSAD
                     TNVCSVAPCASSFDHNVIEFTIKTPATGLYPWQCFVPCGLGWPYGNGGGMSTLGYMGG
                     FLKVVA"
BASE COUNT         1169 a         1764 c         1767 g          868 t
ORIGIN      
        1 cggggcgatc cacgacaggt cgtcgagcac gcgtgtaccg caacagtcgg ccgtacgctc
       61 gcgcgaccgg ccgcggcaac ccgcgtaaca gcggcaccca cggtgcaccg agcacgatca
      121 gcggcggcgc aacgaaggcc agcaggatgt gctgaacgac gtgcgcgctc aggtaggtat
      181 ccgaccagta gtcgatgggc gagacgaccg ccaacagcaa gacgacgagg ccggcccaga
      241 agatgagtgc ctgaccgatc cacggacgga ccggtcggcc ggccttggcg atcgctcgaa
      301 cgtgccgacg caacccgcgg ccgtgcacgg atacaacgac cgcgatcgcc acaatgaacg
      361 ggtcgaagga ccagtgctga agtacggcga tgaagacatc ctctctagat gatcccgcga
      421 gacgtggttg tagtgttcct gcgatgacag accctacgac tccgacgcaa acgccgacgc
      481 agcagccgtc gacgtcgacg cagccgtggt tggcctggat tctgcgccac cgcaagctca
      541 gcatcgcggg cgccgtcgtc atcgtgctcg ctctcgtcgc ggtcgtcgac tggccgcacc
      601 gggcaaccgc gggccagctg cggtccgact tcgcgcagta cgccacgcag gtcaggggcg
      661 acgtgcagtc gtgcagcgtc gaagtcgagg agaccctcag cgcctataac cagatcaccg
      721 ccggcgtcgt acgcgatcga cagacggccg aggcgatcgc gagccagacc gcgctcgact
      781 gcacgccgat gggaaacagt cgaatcgacg acctcggaac ccttcaaccg ccctcgtcgc
      841 tggcaaactt cgccctcgga gcctcgactc agcaactgta cgtctggtgc tttccgaccg
      901 ctgtcgacgt tgcccaggac atcgagcacc tgctgtcgtc gcccggtgat cccgcgacct
      961 tggccgacgt gaagacccgc ctggcccaga tcaactcggc cggcgcgagc gcgcaaacgg
     1021 tattcaacaa ggccgccggg agcctcaaca cgccgccggt cgacttcggt ctcgaccagg
     1081 tacgggcgag cgttctggtc ggctgacagc tgatcggtag gcagggcgac gggagctagt
     1141 tcccgcggcg ctggaagaag ccccaaagca ccgcggcaag gaacaacgag accgggaaga
     1201 tgtatgtcat gaactggctt gtgtgcatcg ctgcctctca gttcagcacg gagaagagta
     1261 agaagagcac gatagcgacg accgagacga agacgacgac tgccttcgcg ccaagtgctg
     1321 agccggcgaa cgcaatctgg atgcgatcgt cacggttcgg cccttccggt gacagcaccc
     1381 accgtacgcg caacgaccgt gccagcaacg tctcgagcca gtaggccgtg acaatgcagt
     1441 agatcgcgaa tagtggcatt gtcgcaacgt acaccgacgc gtagcctgac gagcccggcg
     1501 cccaaccgag gctcggcata ctcgcgagct gcatgatccc tgccgcgagg aacagagccg
     1561 tggcgacacc gcacgcgacc cgcctgccac gaccatgtgt aaaaccagaa gagtgcggcg
     1621 ccgacgagca ccatcaccag cacggggacg ctgatgaagt cagacggtcg ctgccccggt
     1681 gcgcgccaga gcgcgtcgtt gtcgagggag tgcaggtaga agtaggcaaa gacggcagat
     1741 ccgaacagca tcgctgacag cgccacgaac agacgcgagc cggtccagta agcgccacga
     1801 gtcgacgcac gctcaagact gagatctcgc gcgcgagcct cagcctcacc tggcgccatc
     1861 gcaccgccgg cagtcgtcgt catgtcactt cacctcttcc ggctgagtcg ggatgccggg
     1921 cggcgccgcg ccgatatagg ccatcggccc ggcgccggtg ccgtattcgt acgggctcga
     1981 cacgacggtc ggcacgtgcg cgaagttgta cgcgaccggc ggagtcgagg tcatccactc
     2041 gaggccgcgc gaaccccacg ggttgccgac cggctcggtc ggacgcgggt cgacgaacat
     2101 cgtcttcacg aagttgtaaa cgaagaccag catcgacagg ccgatcaggt aggccgagat
     2161 cgagacccaa tcgttgaggc cctgcaacgc cttctcgtac tcgaagacgc gtcgcggcat
     2221 gcccagctcg ccgatagcaa acagcggtag gaacgtcgag ttgaagaaga cgaaaagtgc
     2281 ccagaagtga atccggccga gcttttgatt gagttggcga ccggtcatct tcggcagcca
     2341 gtagtacacg ccgccgaaga acgcaaagat gagcccgccc atgatcgtgt agtggaagtg
     2401 cgccatcacg aagaaactgc cgtgcgcgac agcatcggtc gggacgtcgg acaagaagac
     2461 gccggagata ccaccgatca ggaagttgaa gagcatccca agcacgaaca gcatcgggac
     2521 ggtaagtcgt aatcgtgcac gccacaacgt gccgaccgcg acgaggtaga tgatacccgt
     2581 cgggatcgag atcaactcgg tcgtcaacat cacgaccggc cgcatgtccg ggttcatgcc
     2641 gctgtcgaac aggtgatgct gccagacgaa gaatgacagc gtcccgacgc cgaccatgcc
     2701 ggcgaccgcg gttcgatacc cgaacagcgg cttgcgcgcg aaaaccgtga gcaattccaa
     2761 tgcaatgccg aagccgggaa gcgcgaggat gtagacctcg gggtgcccga agaaccagaa
     2821 cagattctcc cacaggtacg agctgccacc gtggctcgcg tcgaacaggg ccgtctgtac
     2881 ggtgcgatcc atcagggaca tatatgtcgc agcgaccaac acgggcgccg cgagcaccat
     2941 gagcattgcc gttacgagca tcgaccagac aaagatcggc acgcggctcc aagtcatgcc
     3001 cggcgcccga tagttgatga cagtgcaaat gatgttgaac ccgccgacga tcatcgagaa
     3061 cgccatcagg ccgaaggcaa cgagataggc gtcttggcct tggcctgctt cgatcgacag
     3121 cggcgcgtat ccggtccagc cagtcgggaa accgccgacg atgatgccag acagcaagac
     3181 gataaacgcg gccggcgtga tccagaacga cagcgcctcg aggcgcgggt acgccatgcg
     3241 gcgcgagccg atcatcaatg gcaccaggta gttgccgaac gggccgagga tgaccgacgt
     3301 catcatcatc atcatcatcg tgccgtgttc gccaacaacc tcgagatacg tgcccggctg
     3361 aagaatgtgg cgactcggcg acagcagctc agaccgaatc gccatggcga acagcccggc
     3421 gacgaagaag tacacgagca tcgcgaccag gtactgcacg ccgacgactt tgtggtcgag
     3481 ggtgtagctg aagtacgacc ggatggtggg ctttcgctcg tgagcccctt ccgcttcgct
     3541 tgccggggcg tagccgaaca gcttctggaa ggggtagttc agcgcgccga tgccggccag
     3601 ccagccaaag gtggcgaaca ggtaaccaag cacaagtgcg tggttgttct gaccctcgcc
     3661 ctgcacctgc tgataggtat gcgcgatcca gttgccaacc atgtgcccga tcgcgtagcc
     3721 ggcagccgca ccgacgaacg cgagtaacag gttcgggccg agccaccatg gccgcaccgc
     3781 gcgcgatgcg cgtggtccgc cggggttacc tgccgtgtcg accgccgggg gcgccgtcgt
     3841 atccattgcc atgtgtcgcg gcctcctacg gaatcgcgct gggcgaggcg ggaccaggcg
     3901 gcgggccgct ggggagcgga tcttggcctg ggtcgtaata gccgccgtcc gactgcggga
     3961 gatagatatc cgcatacggc ggcaggaagg gaatcaactg ctggtaggtc gcgctggcgt
     4021 ttgtgaccca cgtcttgaag tcggcgggac tcacgacctt gcccgtcgtc gtcatcgcgc
     4081 cgtgccagag tccgcacaac tcgtcgcacc ggatattgac cgagccgatc ttgtcggcgg
     4141 tggcaaacgc cacgttgttc acacccgggt tcgcgtccgc cttcacacca aggttcacag
     4201 cccagaacga gtgaatgacg tcgagggagg tcacgctgta ctgaacctcc tggccggcgg
     4261 ggacgaccat ctgcgcggtt tcgaggccac cgtattgcgg atagcggaac gtccatcgcc
     4321 attgctgccc gatgacttgg acgacgagga gtttggtctg gctcacgttg gagctgtagc
     4381 ccgtcggcga aaagatcgga acctggccgg agcccgttcc agcacctgcg ttggtatcga
     4441 gttcgacagt cccgaacacg aacagcgaaa tcacggtcac tgaggtgata ccgagccagg
     4501 ccgcttggaa tccgcggtgt cccttcatcg gcgggccatc gccgacctca tcgaccggcg
     4561 tgctcgactt cactcgccag ttcgtgagca cgtagcccac gaacacccag acaaagacga
     4621 agactgggac ggcgatagtg cctagtaccc gattatcgaa ctgctgcacg cgcgccgcgt
     4681 cggagtcttt gcccggcggc atgtgcgggc cccacacgaa gtagaccagc agcatgccga
     4741 tgaccgagag gacgatccag atcacagcga tgcggaggat gtgctgccgg tcggtgcgct
     4801 ctgggcgcac ggtcggctcc gagctcgctg gctgcgtcat caggccacga ccttcaggaa
     4861 gccgcccata tagccaagcg tggacatccc gcccccgttt ccgtacggcc agccgaggcc
     4921 acacgggacg aagcactgcc atgggtagag cccggtcgcc ggtgtcttga tggtgaactc
     4981 gatgacgttg tggtcgaacg atgacgcgca cggcgccaca ctgcacacgt tggtgtcggc
     5041 ggacgggata cccggcatcg gcaccgagat accgagcgtc gggacggcga aggtgtggcc
     5101 gatcccgttg tcggtgttgt cgttgatgat ccgcaccggc gatccgccat tgagaacctc
     5161 ggtgccgtcg accgtgccct gcacctgatc cataaacgag ttccgcagcg tgctgccggt
     5221 gtcgtattga tcaatcgtga agtgcacgag tgtgtttgcc ttcagttcga acatcgtcgt
     5281 gtgcacccat ttgccattga tgtccgccag gtacgagacg tacgtcggat gcggcccata
     5341 accgagcgca gggacggtct gcagatggat ctcctcctgc ccgctgcttc cggtcgtcgc
     5401 agcggcggcg acggtcggcg ggtagtggag gaagtacaag aacaacatgg tcacccccgc
     5461 cacgaagacg gcgaacacga ccccaacggc ggtcgcgcgc ataggtctgg tcatctcgac
     5521 cctcctgtcg gcgaaagcgc gcacatcaca tgagtcatag ccatgacc
//
LOCUS       DATCBJ010000482         2116 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_382972, whole genome shotgun sequence.
ACCESSION   DATCBJ010000482 DATCBJ010000000
VERSION     DATCBJ010000482.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2116)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2116)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2116
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_382972"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(40..207)
                     /locus_tag="VII50_09790"
     CDS             complement(40..207)
                     /locus_tag="VII50_09790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5051182.1"
                     /translation="MFLPHQFIELRLEYAAASLFAILFLARYFRTRTLTPFAVYCLFA
                     GGISIIRFAAF"
     gene            complement(230..>2116)
                     /gene="atzF"
                     /locus_tag="VII50_09795"
     CDS             complement(230..>2116)
                     /gene="atzF"
                     /locus_tag="VII50_09795"
                     /EC_number="3.5.1.54"
                     /inference="COORDINATES: protein motif:HMM:NF006043.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004039 - allophanate hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0019627 - urea metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="allophanate hydrolase"
                     /protein_id="HEY5051183.1"
                     /translation="ADSLHNLDLNQTRDAQHSLESPPNGDRLGSLQLAVLSAAYLSGA
                     LDPRELADVVHARASARLADNAWIHLRSAAELRDDATEILRRWPDRNDRPALFGVPIA
                     IKDNVDVAGLPTTAGCADFSYVPATSASLVRRLIDSGALIIGKTNLDQFATGLTGTRS
                     PYGVCTSPFDNSRVTGGSSSGSALAVATGVVSVAIGTDTAGSGRVPAAFTNTVGLKPS
                     RGLVSSRGIVPACRSLDCPSVFALSVADTWTVLQVIAGDDAEDAWTRPFPPLPAGVIT
                     PPARQRIGVPLRGQLSVDADLAVAFASATATLANAGHTILDVDIEPFLLAGELMYDGP
                     WVVERLTDLEQFFTKHPHSIHPVTRDVLSAAQEKTSTDVFRGIHELAELAQRTRSVWA
                     EIDVLMLPTTVTWPTISAALADPHRVNKTLGRCTTFCNLLDLAAVAVPSALSDNGSPA
                     SVTLYGPSGTDSLLASVGAEFHAALHLPTGATSYYLADVPPSEVVAAPAAVTTLLAVA
                     GAHRRGHPLHDQLLALGATYSETTWTAAAYRMYALEGAGPRRPGLVRVDSGGEAIEVE
                     LFVVPVDMLGTLLTQIQAPLGLGRLTLADGRSVSGFLCEAYVATTTHDITKFGSWPAY
                     LETT"
BASE COUNT          354 a          612 c          790 g          360 t
ORIGIN      
        1 cgtttggcgc gcgatcgaag ttgtggggcc cggtctctgt cagaacgcgg cgaatcggat
       61 gatgctgatg cctcccgcga acaagcagta cacggcgaac ggagtgagag tgcgggtgcg
      121 gaagtagcgt gccaagaaca ggatcgcgaa cagggacgcg gcggcgtatt caagacggag
      181 ttcgatgaac tgatgaggta gaaacaccaa gtaccgcgat ctccgcccat tacgtcgttt
      241 cgaggtatgc gggccagctg ccgaacttgg tgatgtcgtg cgtcgtcgtc gcgacatagg
      301 cctcgcagag gaagccggac actgatcgtc cgtcggcgag cgtgagtctg ccaagcccca
      361 ggggtgcctg tatctgtgtg aggagtgtgc ccagcatgtc gacggggacg acgaagagtt
      421 ccacttcgat agcttcgccc ccactgtcga cgcgtacgag gccgggtcga cgcgggcctg
      481 caccttcgag tgcgtacatg cggtacgccg ccgccgtcca ggttgtctcg ctatacgtcg
      541 cgccgagtgc gagaagttgg tcgtggagcg ggtgcccgcg gcggtgcgcg ccagcgaccg
      601 cgagcagtgt cgtaacagcc gccggcgccg cgacgacctc agacggtggc acgtccgcca
      661 agtagtagct ggttgcgccg gttggcaggt ggagtgccgc atggaactcc gcgccgaccg
      721 aggcgagtag tgagtcggtg ccgctgggcc cgtagagcgt gacgctcgcg gggctcccgt
      781 tgtccgacaa tgcactgggg acggcgactg ctgccaggtc gagcaggttg caaaacgtcg
      841 tgcagcgacc gagtgtcttg ttcacacggt gggggtcggc aagtgcggcg gagatcgtgg
      901 gccaggtgac ggtggtcggc aacatcaaga cgtcgatctc agcccacacc gaacgcgtcc
      961 gctgcgctag ctcagccagc tcgtggatac cacggaacac atccgtcgat gtcttttcct
     1021 gcgccgccga tagcacgtca cgggtgacgg gatggatgct gtgcggatgc ttggtgaaga
     1081 actgttcgag gtcggtcagt cgttcgacga cccacggccc gtcgtacatc agttcaccgg
     1141 ccagcaggaa cggttcgatg tcgacgtcga ggatggtgtg cccggcgttg gcgagtgtcg
     1201 cggtcgcgga ggcaaacgcg acggcgaggt cggcgtcgac gctgagttgg ccgcgcaggg
     1261 ggacgccgat gcgctgccgg gcaggtggcg tgatgactcc cgcgggtagc ggcgggaacg
     1321 gccgtgtcca ggcatcctcg gcgtcgtcgc ccgcgatcac ctgcaggacg gtccacgtgt
     1381 cggcaacgga gagcgcgaac acgctagggc agtcaagaga ccggcacgcc ggcacgatgc
     1441 cccgtgaact gaccagcccg cggcttggtt tgagtccgac cgtgtttgtg aacgctgccg
     1501 gcacgcggcc tgagcctgct gtgtcggtgc cgatggcgac cgatacgact ccggttgcga
     1561 cggcgagcgc cgagccggag ctcgagccgc cggttacgcg gctgttgtcg aacggggagg
     1621 tgcacacccc gtaggggctt cgtgtgccgg tcaggcccgt ggcgaactgg tcgaggtttg
     1681 tcttgccgat gatgagcgcg ccgctatcga tgagtcgccg caccaggctg gcggaggtag
     1741 cgggtacgta actgaagtcg gcgcagcctg ccgtggtggg caatccggcg acatcgacgt
     1801 tgtctttgat ggcgatcggg acgccgaaga gcgctggccg atcgttgcgg tcgggccagc
     1861 gacgcaggat ctccgtagcg tcgtcgcgca gctctgcggc agagcgcagg tggatccacg
     1921 cgttgtcggc gaggcgcgcg gacgcgcggg catgcacgac gtcggcaagc tcgcgcgggt
     1981 cgagcgcacc tgacaagtac gcagccgaga ggacggcgag ctgcaagctt ccgagccggt
     2041 cgccgttggg cggggattcg aggctgtgct gcgcgtcgcg ggtttggttg aggtcgaggt
     2101 tgtggagcga gtcagc
//
LOCUS       DATCBJ010000483         4865 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_268877, whole genome shotgun sequence.
ACCESSION   DATCBJ010000483 DATCBJ010000000
VERSION     DATCBJ010000483.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 4865)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4865)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4865
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_268877"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(26..1243)
                     /gene="hemW"
                     /locus_tag="VII50_09800"
     CDS             complement(26..1243)
                     /gene="hemW"
                     /locus_tag="VII50_09800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HEY5051184.1"
                     /translation="MPSTLPDGDPQPSDGRLPPDVLATVGSRPFSVYIHVPYCATRCG
                     YCDFNTYTASELGGGASQTSYARDAIAELRLARDVLGDADLPVQTVFFGGGTPTLLPV
                     ADLAAMLTAVRDLFGLARDAEVTTEANPESVDARSLEALRVAGFTRVSLGMQSVRPHV
                     LAVLDRVHTPGASAVRVAEARAAGFEHVSLDLIYGTPGESDDDWRASLSAAIAAEPDH
                     ISVYSLIVEDGTRLAGRVARGELPRPDDDVLADRYLIADEVLAAAGFGWYEVSNWARP
                     GGQCRHNLAYWRSADWWGAGPGAHSHVGGVRWWNVKHPTAYAARVSAGVSPGYARECL
                     DTSTRRVERVLLQLRLAEGLDLVELDDAGRTVARAEVASGLLDPVAFEGGRGVLTREG
                     RLLADGVVRRLLG"
     gene            1334..1762
                     /locus_tag="VII50_09805"
     CDS             1334..1762
                     /locus_tag="VII50_09805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5051185.1"
                     /translation="MFWLALWLTATVAICVLALLPVNRVALHVKFAEAVGRGWRRFRP
                     APAQPVGQPIEDIAASLRRLQRWLDSYAESRPIPGKATKVIAATSAYDRVLVEACRAL
                     QVPEGLDGTDGLDREAERLRMQSALIDGGLVLRGRREAER"
     gene            complement(1769..2407)
                     /locus_tag="VII50_09810"
     CDS             complement(1769..2407)
                     /locus_tag="VII50_09810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HEY5051186.1"
                     /translation="MTRATVVAVNVGRAMPMPGKSGVSSIDKRAVEGRVALRELGLDG
                     DSQSDTANHGGPEQAVYAYAMEDLDHWAGLLGRELRPGQFGENLSTRGLDVTGALIGE
                     TWRIGSTLLQVSRPRFPCVVFQNWLAEPQWVKRFTDDGRPGAYLRVLEEGELGAGDDV
                     VVEDRPDHDVTIGLVFRARTTDRSLIPRMLAATQLPKADYQFALRHAAEGSS"
     gene            complement(2412..4265)
                     /gene="lepA"
                     /locus_tag="VII50_09815"
     CDS             complement(2412..4265)
                     /gene="lepA"
                     /locus_tag="VII50_09815"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HEY5051187.1"
                     /translation="MVGASPTREKRVPLQRTDPATIRNFCIIAHIDHGKSTLADRMLQ
                     LTGVVDERSMRAQYLDRMDIERERGITIKSQAVRLPWKVAGANYVLNMIDTPGHVDFT
                     YEVSRSLAACEGAVLLVDAAQGIEAQTLANLYLAIQSDLRIIPVLNKIDLPAAQPDRY
                     AEELAHVIGCEPGDVLRVSAKTGSGVRELLDHVVHTIPPPVGDFEAPPRALIFDSVYD
                     VYRGVVTYVRVVDGYLSSRERIKMLSTGTTHELLEVGVISPEPIPTEGLGVGEVGYLI
                     TGVKDVRQSRVGDTVTVVSRAATEPLAEYTLPKPMVYSGLYPIDGSDYPLLRDALDRL
                     QLNDAALAYEPETSAALGFGFRCGFLGLLHLEIVRERLEREFNLDLISTAPNVVYRVI
                     REDGKETTVTNPSEYPEGKIAEVYEPVVRATVLSPSDYVGTIMELCQNRRGNLLGMDY
                     LSADRVELRYTMPMAEIAFDFFDQLKSRTRGYASLDYEPTGEQAGDLVKVDILLQGEA
                     VDAFSAIVHKDKAYAYGVEMTKKLRELIPRQQFEVPIQAAIGSRIIARESIRAIRKDV
                     LAKCYGGDISRKRKLLEKQKEGKKRMKMVGRVEVPQEAFIAALSTAPAAKD"
     gene            complement(4317..>4865)
                     /locus_tag="VII50_09820"
     CDS             complement(4317..>4865)
                     /locus_tag="VII50_09820"
                     /inference="COORDINATES: protein motif:HMM:NF016840.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEY5051188.1"
                     /translation="GSALTDIDEQGRPEPPLAGDETATILGFLDYQRATLAWKCRGVD
                     ADGLTATVGGSSMTLGGMLKHLSYVEDEWFSRWLPGHDRLPPWNTVDWKADPDWEWNS
                     AADDTPDQLFEFWHGAVERSRARVAAALAGGGLDTLARRTWPDGRAPSLRWIVVHMIE
                     EYARHNGHADLIRESIDGLVGE"
BASE COUNT          787 a         1699 c         1584 g          795 t
ORIGIN      
        1 tccgtgcagg ccgctaagcc cagcgttacc cgagcaagcg tcgtacgacg ccgtccgcga
       61 gtaaccgacc ctcgcgagtg agcacgccgc gacccccctc gaacgccacc ggatcgagca
      121 agccggacgc aacctcggcg cgcgccacgg tgcgaccggc atcgtcaagc tcgacaaggt
      181 cgaggccctc cgccagtcgc aactgcaaca gcacccgctc gacccgccga gttgaagtgt
      241 cgagacattc gcgcgcatag cctgggctga cgccggccga tacccgcgcc gcgtaggcgg
      301 tggggtgttt cacgttccac cagcgcacgc ccccgacgtg actgtgggcg ccgggcccgg
      361 cgccccacca gtccgcgctg cgccaatagg ccaggttgtg tcgacactgc ccccccggcc
      421 tcgcccagtt cgacacctcg taccagccga agcccgcagc ggccaacacc tcgtcggcga
      481 tcaggtagcg gtctgccagc acatcgtcgt ccggccgtgg tagctcaccg cgcgccaccc
      541 ggccggcaag tcgcgtgccg tcctcaacga tcaacgagta cacgctgatg tggtccggtt
      601 cggccgctat agctgcggac agcgacgccc gccagtcgtc gtccgattca ccgggggtgc
      661 cgtagatcag atcgagactg acgtgctcga agcccgcggc tctcgcttcg gcgactcgca
      721 cagccgacgc acctggcgtg tgcactcggt cgagtacggc gagcacgtgc ggacgtaccg
      781 actgcatgcc gaggctgacc cgcgtgaagc ccgctacgcg cagcgcctcc agcgagcggg
      841 cgtccaccga ttcggggttg gcctccgtcg tcacctccgc gtcacgagca agaccgaaga
      901 ggtcgcggac ggcggtgagc atcgcggcga gatcggcgac cggcagcagg gtcggagtgc
      961 caccgccgaa gaacacggtc tgcaccggca gatcggcgtc gccaagaacg tcgcgggcaa
     1021 gtctcagttc ggcgatcgcg tctcgtgcgt aggacgtctg gctcgcgccg ccgccgagct
     1081 cggaggccgt atacgtgttg aagtcgcagt aaccgcagcg ggtcgcgcaa tacggcacgt
     1141 ggatgtagac gctgaacggc cgcgatccga cagtagccag gacgtcgggg ggcagcctgc
     1201 cgtcggacgg ctgcgggtcg ccgtccggca atgtcgatgg cacggtcaca ttctgcgcac
     1261 agcaccgaac cgactagtgc ggttggcgcg tagagcgagc agaatcggga ctgcccgagg
     1321 aggcggcagg ctggtgttct ggctcgcgct atggcttacg gcgacggtcg caatctgcgt
     1381 gctcgcgttg ctgccggtca accgcgtcgc cctgcacgtg aagttcgccg aggccgtggg
     1441 ccgcggttgg cggcgattcc ggccggcacc cgcccaacct gtcgggcagc caatcgagga
     1501 catcgccgcg agcctgcgcc ggctccagcg ctggcttgat agctacgccg agtctcgacc
     1561 gatccctggc aaagccacca aggtgatcgc ggcgacaagc gcttatgacc gcgtgctggt
     1621 cgaggcgtgt cgggcgctcc aggtacctga gggcctcgac ggcaccgacg gactcgatcg
     1681 cgaagcggag cggttgcgca tgcagtcggc actcatcgac ggcggcctcg ttttgcgcgg
     1741 gcgtcgcgaa gcggaacgct gaccgtcgct aggacgagcc ctctgcggca tgccggaggg
     1801 cgaactggta gtcggccttg gggagttggg tcgcggcgag catccgcggg atcaggctgc
     1861 ggtccgtcgt ccgcgcacga aagaccaggc cgatggtgac atcgtgatcc ggcctgtctt
     1921 cgacgacgac gtcatcgccg gcgcctagct cgccctcctc gagcacccgc aagtaggcgc
     1981 ctggccggcc gtcgtcggtg aaccgcttga cccactgcgg ctctgcgagc cagttctgga
     2041 agacgacgca cggaaacctc gggcgtgaca cctgaagcag cgtgctgccg atgcgccacg
     2101 tctcaccgat cagcgccccg gtgacatcga gcccgcgggt cgagaggttc tcgccgaact
     2161 ggccaggccg cagctcgcga cccagcagac ccgcccagtg gtcgaggtct tccatggcgt
     2221 aggcgtagac agcctgctcg ggtccgccgt ggttcgcggt gtccgactgc gagtcgccgt
     2281 ccagcccgag ctcacgcagc gcaacccgtc cctcgacggc gcgcttgtcg atcgagctca
     2341 cccccgactt gcccggcatc ggcatcgcgc gcccgacatt gacggctacc accgtcgccc
     2401 gcgtcatgcc gtcagtcctt cgcagccggg gccgtcgaca acgcggcgat gaatgcctcc
     2461 tgcggaacct cgacgcggcc gaccattttc atccgcttct tgccttcctt ctgcttttcc
     2521 agcagcttgc gcttgcggct gatgtcaccg ccgtagcact ttgccagcac gtcctttcgg
     2581 atcgcgcgaa tgctctccct ggcaatgatg cgcgacccga tcgccgcctg gatcggcacc
     2641 tcgaactgct ggcgtgggat gagctcgcgc aacttcttcg tcatctcgac gccgtacgcg
     2701 taggccttgt ccttgtgcac gatcgcgctg aacgcgtcga ccgcttcgcc ctgcaggagg
     2761 atgtcgacct tgaccaggtc gccggcctgc tcgccggtcg gctcgtagtc gaggctcgcg
     2821 taaccccggg tccgtgactt cagctgatcg aagaagtcga acgcgatctc ggccatcggc
     2881 atcgtgtagc gcaactcgac gcgatcggcc gacaggtagt ccatgccgag caggttgccg
     2941 cgcctgttct ggcacagttc catgatcgtg ccgacgtagt cgctcggcga gagcacggtg
     3001 gcccgcacca ccggctcgta gacctccgcg atcttcccct cggggtactc gctcgggttc
     3061 gtgacggtcg tctccttgcc gtcctcgcga atgacccggt agacgacgtt gggcgcggtc
     3121 gagatcaggt ctaggttgaa ttcacgctcc agccgctcac gcacaatctc caagtgcagc
     3181 aggcccagga agccgcagcg gaacccgaac ccgagcgcgg cgctggtctc cggctcgtag
     3241 gcaagggctg catcgttgag ctgcaagcgg tcgagcgcgt cacgcaacag cggatagtcg
     3301 ctgccgtcga tcggatagag cccggagtag accatcggct tgggcagcgt gtactcagca
     3361 agcggctcgg tcgccgcccg actgaccacc gttacggtgt cgcccactcg tgactgccga
     3421 acgtccttca ccccggtgat gaggtagccg acctcgccga cgccgagtcc ctcggtcggt
     3481 atcggctccg gggagataac cccgacctcg agcagctcgt gggtggtccc ggtcgagagc
     3541 atcttgatgc gctcccggct cgacaggtag ccatcgacga cccgcacata ggtgacaacg
     3601 ccgcggtaca cgtcgtacac cgagtcgaag atgagcgcgc gaggcggcgc ctcgaagtcg
     3661 ccaaccggag gcgggatcgt gtgcacgacg tggtcgagca gttcgcgaac cccggagccg
     3721 gtctttgcgc tgacccgcag cacgtcaccg ggctcgcagc cgatgacatg cgcgagctcc
     3781 tcggcgtacc ggtcgggctg cgccgccgga aggtcgatct tgttcagcac cgggatgatg
     3841 cgcaggtcgc tctggatcgc caggtagaga ttggccagcg tctgcgcctc gatcccctgc
     3901 gccgcgtcga ccagcagcac cgccccctcg cacgccgcga gcgaccggga cacctcatag
     3961 gtgaagtcga cgtggcccgg ggtgtcgatc atgttcagca catagttggc gcccgcaacc
     4021 ttccagggca gccggaccgc ctggctcttg atcgtgatgc cgcgctcgcg ctcgatgtcc
     4081 atccggtcga gatattgggc gcgcatcgag cgctcgtcga ccacgcccgt gagttgcagc
     4141 atgcggtcgg ccagcgtcga cttgccgtga tcgatgtggg cgatgatgca gaagttgcgg
     4201 atcgtcgccg gatcggtgcg ttgtagcggc acgcgcttct ctcgagtcgg ggatgccccc
     4261 accatgatcc catgccggac cgggaagccc cgcgatcaag ctccgagctg ccgcgcctac
     4321 tcgcccacga gcccgtcgat cgactccctg atcaggtcgg cgtgcccgtt gtgccgcgcg
     4381 tactcctcga tcatgtgcac aacgatccaa cgcaggctcg gtgcccggcc gtcaggccag
     4441 gtgcgccgcg cgagggtgtc gaggcccccg ccggccaacg ccgcggctac ccgcgcgcgc
     4501 gagcgttcca ccgcgccatg ccagaactcg aagagctgat ccggggtgtc gtcggcggcc
     4561 gagttccact cccaatcggg atcggctttc cagtcgacgg tgttccaggg tggcagcctg
     4621 tcgtgcccag gcagccaccg cgagaaccat tcgtcctcga cgtaggacag gtgcttgagc
     4681 atgccgccca aagtcatcga cgaccccccg acggtcgctg tgagaccgtc cgcatccacc
     4741 cccctgcact tccaggcgag agttgcgcgc tggtagtcga ggaaaccaag aatcgtggct
     4801 gtctcgtcac ccgccaacgg cggctcagga cggccctgct cgtcgatgtc agtcaaggca
     4861 ctccc
//
LOCUS       DATCBJ010000484         2705 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_57269, whole genome shotgun sequence.
ACCESSION   DATCBJ010000484 DATCBJ010000000
VERSION     DATCBJ010000484.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2705)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2705)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2705
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_57269"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..423
                     /locus_tag="VII50_09825"
     CDS             <1..423
                     /locus_tag="VII50_09825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
                     /protein_id="HEY5051189.1"
                     /translation="PRVGFLPPTDPRVLGTIAAIQTGLADDGLIKRYQTSDADDGLPG
                     GEGLFLACSFWLVDALHLAGQQQQATELFERLLKLRNDVGLLSEEWDPTTGRLLGNTP
                     QAFSHFPLVTSALQLHAGRPHRSNQPIRHPGTPNPTPA"
     gene            472..2217
                     /locus_tag="VII50_09830"
     CDS             472..2217
                     /locus_tag="VII50_09830"
                     /inference="COORDINATES: protein motif:HMM:NF013109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015103 - inorganic anion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HEY5051190.1"
                     /translation="MTPATGLAAQVLRVRSVLFSSLRGYHASWAVGDLVAALTLLTIA
                     VPEQLATSRLAGMPPITGLYAFVAGSVVFALLGSNPQMSVGGDSTIAPLFAVGIAGLA
                     ATGSPHYIALVGILAVMVGVLVALIGVLRLGWIAEFLSAPITSGFLAGVAVIIVVHQL
                     PDLLGLPGGGGSTLHRIGYAVTHMNLLNGWTLGIGIGVFAVVAIAERVDRRLPGALIG
                     LIASIILVGALQLHTHGVAVLGALAHGAPHLGLHGLSWSVLRSVAPLAGVVALVVVSQ
                     TAATTRAFADQGDYQVDVNRDFLGVGAGSIVAGLVGAFPVNASPPRTAAVAAASGRTQ
                     AAGLVAAGAVVVLIPAAALLKNLPLATLAAVLIFIATRIFHVRDLITIAGYDRVEFAL
                     ALITLLTVGLVGVEQGIAVAVALAILDRTRLSARPQLHVLGRIPSTTSWAPLNGTDHA
                     TQLPGVLVVLFATPIWYANAIHFRTALSAAVDSASHPLKGLVLDTIGMSDIDYTGSRA
                     LRQILDQLDRDGVTFAMARTGDRVRANLARSGLLQRIGDDHLYPSVDEAVTAVHRNNP
                     DADTSPARPTPGQAS"
BASE COUNT          438 a          988 c          847 g          432 t
ORIGIN      
        1 ccccgcgtcg gcttcctacc cccaaccgat cccagagtcc ttggcaccat cgccgcaatc
       61 caaactgggc tcgccgatga cggactgatc aagcgctacc aaaccagcga cgccgacgac
      121 ggcctccccg gcggtgaagg gcttttcctg gcctgctcat tctggctcgt cgacgccctg
      181 cacctcgccg gacaacagca acaagccacc gagctattcg aacgtctcct caaactgcgc
      241 aacgacgtcg gactgctcag cgaggaatgg gacccaacaa ccggccggct gctcggcaac
      301 accccccaag cattcagcca cttcccgttg gtgaccagcg ccctgcagct acatgcgggc
      361 cggccacacc gaagcaacca acccatccgg caccccggca cacctaaccc gacacccgcg
      421 tgacgccttt ggggaatggc gggtgacggt cgaaggcttg atgatgggcc ggtgactcca
      481 gcaaccgggc tcgccgcgca ggtgttgcgg gtgcggtcgg tgctgttctc ttcgctgcgc
      541 ggctaccacg cgtcgtgggc ggtcggcgac ctggtggccg cgctgacctt gctcacgatc
      601 gccgtgcccg agcagctggc cacctcccgg ctcgccggta tgccgccgat caccggcttg
      661 tatgcgttcg tggccggcag tgtggtgttc gcgttgctgg ggtccaatcc gcagatgtcg
      721 gtcggaggcg attccaccat cgccccgttg ttcgcggtcg gcatcgcagg cttggcggcg
      781 accggctcgc cccactacat cgcgctggtc ggaatccttg cggtgatggt gggagtcctc
      841 gtcgcgctga tcggcgtgct gcgactgggg tggatcgcgg agttcctgtc cgcgccgatc
      901 accagcggct ttctggccgg ggtcgcggtg atcatcgtcg tacaccagct acctgacctg
      961 ctcggcctgc caggtggcgg cggcagcacg ctgcaccgca tcggatacgc agtcacccac
     1021 atgaacctgc tcaacgggtg gacgttgggc atcgggatcg gtgtgttcgc cgtcgtggcg
     1081 atcgccgaac gcgtcgatcg cagactcccc ggggcgctga tcggactgat cgcctcgatc
     1141 atcttggtcg gcgcgctgca actgcacacg cacggcgtgg ctgtgctcgg cgcactggcc
     1201 cacggcgcgc cgcacctcgg cctgcacggc ctgtcatggt cagtgctgcg cagtgtcgcg
     1261 ccactcgccg gcgtcgtcgc cctggttgtc gtcagccaga ccgcggccac gacccgcgcc
     1321 ttcgccgacc agggcgacta ccaagtcgat gtcaaccgcg actttctcgg cgtcggggcc
     1381 ggcagcatcg tcgccggcct cgtcggagca ttcccggtca acgcgagccc accacggacc
     1441 gccgccgtgg ccgcggcgtc cgggcgaact caagcagccg gtctggtcgc ggccggcgcc
     1501 gtcgtggtgc tgatcccggc cgcagccctg ctgaagaacc tgcccctggc gaccctggca
     1561 gcagtcctga tcttcatcgc cacccggatc ttccacgtcc gtgatctcat caccatcgcc
     1621 ggctacgacc gggtcgagtt cgcattggcc ctcatcactt tgttgacggt cggcctcgtg
     1681 ggcgtcgagc agggcatcgc cgtcgcggta gcgctcgcca tcctcgaccg cacccggctc
     1741 agcgcccgcc cgcagctaca cgttttgggc cgcatcccct cgaccaccag ctgggcgccg
     1801 ctgaacggca ccgaccacgc gacacaactg ccaggggtgc tcgtcgtgct gttcgcgacc
     1861 cccatctggt acgccaacgc gatccatttc agaaccgcac tgagcgccgc cgtcgactcc
     1921 gccagtcacc cgctcaaggg gctcgtgctc gacacgatcg ggatgagcga catcgactac
     1981 accggttcac gcgcactgcg gcagatactc gaccagctcg accgcgacgg ggtcaccttc
     2041 gcgatggcca gaaccggcga ccgggtccgc gccaacctgg cacgcagcgg cctgctccag
     2101 cgcatcggcg acgaccacct ttacccgtcc gtcgacgagg cagtcaccgc cgtgcaccgc
     2161 aacaaccccg acgccgacac gagcccggcc cggccaacac cgggccaggc gtcctagcgg
     2221 gttcgtcgag cggatcacgg cgaggatccc gacgtcgggt cacgacccgt cggagtttgg
     2281 ggatgcctgg gtagtggcga gacacctgaa ctcgcagaca cggaaagcca cgcttgaaga
     2341 acccccgacg gggaagggac acgttgtaac cgtacgcgag gcatcgcgca ccgcttcgtg
     2401 ccgaccagcg tgcgggacac cagggtcgcg accccgcacc gcgcatcgag aagaggatcg
     2461 gcgtcgtcac gatcctcgct gggatccgca ccgggatgcg tgagccgctg acctcgttcg
     2521 tcgtcaccgg ggcgggcata aggacgcctg tgaccagtca cggcagcaac tcgcttccac
     2581 ggtgagtgac tgaccagacg gaccacgatc gcaccgcgct cgactcgacc gaggagcacc
     2641 gcaccatgac agcccgaacc ctggaccccg aactgctgca tcggatggat gcgtactggc
     2701 gggcg
//
LOCUS       DATCBJ010000485         7083 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_416772, whole genome shotgun sequence.
ACCESSION   DATCBJ010000485 DATCBJ010000000
VERSION     DATCBJ010000485.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 7083)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7083)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7083
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_416772"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..195)
                     /locus_tag="VII50_09835"
     CDS             complement(<1..195)
                     /locus_tag="VII50_09835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5051191.1"
                     /translation="MSTQPLPVAKQKAIGLKLDNHRVMWLGGEADDTADNNRTFIYDT
                     RTRTSREAAPIPAAKPMNHFA"
     gene            1046..1495
                     /locus_tag="VII50_09840"
     CDS             1046..1495
                     /locus_tag="VII50_09840"
                     /inference="COORDINATES: protein motif:HMM:TIGR02612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mobile mystery protein A"
                     /protein_id="HEY5051192.1"
                     /translation="MRSAQARDARRALDRRFSQLPPATAFTPPAHGWIKAVRNALGMS
                     AADLAARLQVTPASVSDIERSENDRRIRLDTLERAAAAMGCDLVYALIPHGALTDIVQ
                     HQAQTKVELQVRAVSRAMDLEAQSTQVDDEVIREEMQRLIDSGRLWK"
     gene            1492..2103
                     /locus_tag="VII50_09845"
     CDS             1492..2103
                     /locus_tag="VII50_09845"
                     /inference="COORDINATES: protein motif:HMM:TIGR02613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mobile mystery protein B"
                     /protein_id="HEY5051193.1"
                     /translation="MTPFDGQPDGATPLTDDDVRGLKLSWISTQAELNQAEAENILRG
                     RLWASRIRRRPFWYLRDDRLQELHRRILGDVWDWAGKLRERETNIGIDPHHIRIQLRD
                     LCDDTLAQTDERTNLAYPVDELAVRFHHRLLLIHPFPNGNGRHSRLATDLLVADLGAE
                     PLSWGGAQLTTDTDLRANYLSALRHADSNHDFAPLIAFARRTN"
     gene            2134..2622
                     /locus_tag="VII50_09850"
     CDS             2134..2622
                     /locus_tag="VII50_09850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5051194.1"
                     /translation="MPIKRMKKTTGHSLHVLHTHEVSASSAEDGLSEAPTKESQGGIA
                     TARALASIWSATVVDTDGVRLLDDGTIADATRPQTEGQPVFAAPPPFARWGTGFQLHW
                     PRARTFLGPCSFGHDRLGGQVSFADARFHVGFGYITNFLEGAADGRSAAGIEALRRTM
                     EP"
     gene            complement(3024..4307)
                     /locus_tag="VII50_09855"
     CDS             complement(3024..4307)
                     /locus_tag="VII50_09855"
                     /inference="COORDINATES: protein motif:HMM:NF019350.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HEY5051195.1"
                     /translation="MASVAVLRALPRRPVLVDVFLAACVSLPGLVALPWSSPGISASV
                     ARTIGAIAMLAVAGAVVFARRRWPFGAAAAGAVTLVVAVEAGSFDPNGSSVDGALVTI
                     GLLLWAVVMSFTLGTCRPIRVPLVGVVLVVVAQQWHGVNPFPAVLAIGSYAVGWIVRS
                     RQDLAAALDVRAVELAAEQQRYATEAVRFERARIARELHDVVAHCMSIVVVQASAGQR
                     LPHTDPTHTAATDQILDNIAELTRQAESDLDGLTRLLSREAQPAPALSRGLIDQLVAR
                     AISAGTPVQVNVVGDLATIPSLAASALGRVLQEGLTNAIKHAPGAYVAVRVRTDDWLT
                     SIEIENGAARATSEQLGVPSGGNGLRGLRDRVTTLAGTLSGSPTASGGWRLTAVIPAT
                     DAVAGPPPADRSLDRCSPIELLAVGRREAGGLRLG"
     gene            complement(4300..5415)
                     /locus_tag="VII50_09860"
     CDS             complement(4300..5415)
                     /locus_tag="VII50_09860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5051196.1"
                     /translation="MNNAFGLLRRRVVGVSVGAALFTVLLSAWAVALVSSQHTGRIGA
                     PVAVLIMIVPVGFARRSPFIVATVLAAGTLLNNLFFGHLVRCGPALPAAFFVAYVIAA
                     ATIGRERWGGLTLVLAGVALQCVWDPKLGASQITLMAPATLLFFGAGMSVRSRSVLVT
                     QLRERNAQLIEQRERTAELAVAADQARIRGELVDGLRLQIHEIAQTASGARAGTIPTA
                     ESLTAIEQTGRAALDRMRHIVGTIRSASTQPEPDLDALRDLLRSATTCEVHLSIEGDQ
                     RALPASIELSSYRIVERLISSLDDDPAARAQVRVTFEADALEILVTGPLSSDFDSASG
                     FGGVLDRAALHGGTVRLAEPNGRLDAHVRLPLVTSHG"
     gene            complement(5479..6006)
                     /locus_tag="VII50_09865"
     CDS             complement(5479..6006)
                     /locus_tag="VII50_09865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5051197.1"
                     /translation="MTPLIGLVIAVIAGLLAPSVRGLMLSVLAAMLAATAVQSWDLGA
                     GFGSNPPSTIASVSYWIVQVIIISIICAVAYGIYRLRARRGIRGGRSMVRPQFSGQRG
                     AKVVAWSVSATTAVMVAGCLLVGHFKTHRGQGAGHIPWTGVLGIGIGIVAVVVIAGVL
                     ALGSGRRAAADVQAQ"
     gene            6187..6897
                     /locus_tag="VII50_09870"
     CDS             6187..6897
                     /locus_tag="VII50_09870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009085703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEY5051198.1"
                     /translation="MEQASPAVSRQAPAPIRVVIADDQRLVRTGFRVILEREPDIEVV
                     GEADDGVAAIQQTLDVQPDVVLMDIRMPHLDGFQATRRVLAETGSKVVILTTFDSDEY
                     VYEALRSGASGFLLKDAPADQLIAAVRCAATGDALIDPAVTRRLVTRFAHALRPPTGT
                     PERFAALTARELDILRLIARGLNNAEIAAHLVIEESTVKTHVGRILAKLGLRDRVQAV
                     VLAYETGLISPDGSAESG"
BASE COUNT         1343 a         2383 c         2223 g         1134 t
ORIGIN      
        1 ggcaaagtgg ttcatcggtt tggccgccgg aatcggcgct gcttcccgtg acgttcgagt
       61 gcgcgtgtca tagatgaacg tccggttgtt gtcggccgtg tcgtccgcct cgccgcccag
      121 ccacatcacc cggtgattgt ccaacttcaa accgatagcc ttttgcttgg cgacgggcaa
      181 cggctgggtc gacacagacc aaccaggcac tgcggccgcg ccgcccgtac tggcgccgtc
      241 cgccgcccgc gccgccccag cggttgccga gagacacaat gtgcccgtta gcgcccctgc
      301 gaggagcatc cgaaacgctc gcatcaagac ctccccagaa ccacacccgc gacacccggc
      361 ccagagtccc tctctcgagc tgagggattg gcgattgccg tgcagcctta actgctccgg
      421 acgcgaccac cgcagtggcg atcgccgtaa atccaaggtg ccgctaagga gcagaggcgc
      481 acatccggag aatcctcaga tcaggcgtaa gtagtaccta gatctgcgac gcgaaagaaa
      541 cccgtcaacc gggtccgtag cggggccgta cggccggtaa agtgcggtcc gatacggatc
      601 acagcggtca agatcaaaaa cagatatgcg caggtcagtg cgcgttttca cgctcaatcg
      661 ggaacgtgtc ccgctaggct tccaagctgg ccatgccggt tcgatcccgg tcacccgctt
      721 taccgaaaaa cccttacaca gcaagggttt tcacgcgccc acggccgtcc cgtcatcctg
      781 aaacgtagat catgactccc tagcgaggcc gtaagtacca tgccgctagg gcaggcccca
      841 gtccggcaag ctggccatgc ccgttcgatc cggtcacccg ctcatcccgc tccactgaaa
      901 tccacaaggt cggcagaccg ccttcgccta atattaggct acagccataa ttgtggctgc
      961 tagattcatg gctatgacct cttctgtcat gctattctca tgggtgtagc cattgatttt
     1021 gacagcaacc ctggaggccc taaccatgcg ctcagcccaa gcacgcgacg cccggcgggc
     1081 tctggatcgg cggttcagcc agttgccacc agcgacagcg ttcacgcctc ctgcgcacgg
     1141 ctggatcaaa gccgttcgaa acgcgctggg catgtctgcc gcggacctcg cggctcgttt
     1201 gcaagtcacg ccggccagcg tgagcgacat cgaacggtcg gagaatgacc ggcggatccg
     1261 gctggacacc cttgagcggg ccgccgcggc gatggggtgc gatctggtct acgcactgat
     1321 cccccacggc gccttgaccg acatcgtcca gcaccaggca cagacgaagg tcgaactgca
     1381 ggtgcgcgcg gtgtcgcgcg cgatggacct ggaagcacaa agcacgcagg tcgacgacga
     1441 ggtcatccgc gaggagatgc aacggctcat cgactccggg cggctatgga agtgaccccg
     1501 ttcgacggcc agccggacgg ggccacgcca ctaaccgacg acgacgttcg cgggctcaaa
     1561 ctgtcctgga tctctaccca agccgagctg aaccaggccg aggcagagaa catcctgcgc
     1621 ggacgcctct gggcgtcacg aatcaggcga cggccgttct ggtacctgcg cgacgaccgt
     1681 ctccaggagc tacacaggcg gatccttggt gacgtgtggg actgggctgg gaagctgcgc
     1741 gagcgcgaga ccaacatcgg catcgaccca caccacatcc ggatccagct acgcgatctc
     1801 tgcgacgaca ctctcgcgca aaccgacgaa cgcactaacc tcgcctaccc ggtcgacgaa
     1861 ctagcggtgc gcttccatca ccgactcctg ctcatccacc ccttccccaa cggcaacggc
     1921 agacactcac ggctggccac cgaccttctc gtcgccgacc tcggcgctga acccttgagc
     1981 tggggaggcg cgcaactcac caccgacacc gacctgcggg cgaactacct gtcggcactg
     2041 cgccacgcag acagcaacca cgacttcgcc ccgctgatcg ccttcgcacg ccgcaccaac
     2101 tgacggcaac acaacgccgg cacgacctgc aacatgccga tcaagaggat gaagaagacg
     2161 accggccaca gcctccatgt tcttcatact catgaggtgt ccgcgagcag cgcagaagac
     2221 ggactatccg aggcaccgac gaaagagtcc cagggtggca tcgcgaccgc acgcgcgctg
     2281 gcatcgatct ggtccgccac agttgtggac accgacggcg tccggctact cgatgatgga
     2341 acgatcgccg acgccacccg cccacagacc gaaggtcaac cggtgttcgc ggccccgcct
     2401 ccgtttgcgc gatggggtac cggcttccaa ctccactggc cgcgcgcacg aacgttcctc
     2461 ggcccgtgtt catttgggca cgaccgtctc ggcggacagg tcagctttgc tgatgctcgg
     2521 ttccacgtcg ggttcggcta catcacgaac ttcctggagg gtgccgccga cggaaggtca
     2581 gccgccggca tcgaagcatt acgccgcacc atggagcctt agcgcggacg attcaggcgt
     2641 gcatcagatt gaaaagcggc gacccagacc gaagtttcaa cgacttgttc gagcgaccca
     2701 cggcacgggc ttgaccctcg aaatcgccgc gccgtcgccg gactgctgct cggggtcctc
     2761 gctacgtcgt tgatttagat ctatcgcagc cagaacaccg gcaggcccgg agccgcgcag
     2821 acttcggcta cgccgccgtt tggactgtcg tgatcggtgc acgtgccgcc ttttcctacg
     2881 ggtctagcca ctggttccat acggagctgg gcacctggat gagcgctcac gacgtcagcg
     2941 tcaatgccgt gactaatgcc ctcatcctga tggccgtcgc gaggggctgc gccggcacgg
     3001 tcggcatggc gggttccgac gcgctacccc aggcggagac cgcccgcctc gcgtcggccg
     3061 accgcgagaa gctctatggg actgcatcga tctaaagatc ggtctgcagg gggtggcccc
     3121 gcgacggcgt ccgtggcggg gatgacggca gtcaagcgcc agcctccgga cgccgtcggc
     3181 gaaccgctca gggtgcctgc caacgtggtc acgcgatcgc ggagaccacg aagcccgttg
     3241 ccgccgctcg ggacgcccag ttgctccgat gtcgctcggg cggctccgtt ctcgatctcg
     3301 atcgatgtca gccagtcatc ggttcgcacg cggacagcaa cgtaggctcc cggagcgtgc
     3361 ttgatggcgt tggtcaatcc ctcctgcagg acacgaccca gcgccgatgc agcgagcgac
     3421 gggatcgtgg cgagatcacc cacgacgttc acttgtaccg gcgtccccgc ggagattgca
     3481 cgagcgacga gctgatcgat caagccgcga ctcaaggctg gtgctggttg cgcttcgcgc
     3541 gacaacaggc gggtgaggcc atcgaggtcc gactcggctt gccgcgtcaa ctcggcgatg
     3601 ttgtcgagta tctgatcggt cgcggcagtg tgggtcggat cggtgtgggg tagccgctgc
     3661 ccagccgatg cttgcacgac cacgatgctc atgcagtgcg cgacgacatc gtgtagttca
     3721 cgtgcgatgc gggcacgttc gaagcgaacc gcctcggtcg cgtaccgctg ttgttcggcg
     3781 gcaagttcaa cagcccggac gtccagtgcc gcggcgagat cttgtcgcga ccgtacgatc
     3841 caccccacgg catacgagcc gatcgccaac actgcaggga acgggttgac accgtgccac
     3901 tgctgggcaa cgaccaccag caccacgccg accaggggca cgcgtatcgg ccggcaggtg
     3961 ccgagcgtga acgacatcac cacggcccac agcagtaagc cgatggtcac gagtgcgccg
     4021 tcgacactcg aaccgttcgg atcgaagctg cccgcctcga ccgcaacgac gagggtaacc
     4081 gcgccagcag cggccgcacc gaacggccac cggcggcgcg cgaatacgac cgccccggca
     4141 acggcgagca tggcgattgc tccgatggtt ctcgcgacgc tggcggatat cccggggctc
     4201 gaccacggca gggccacgag tccaggtagg gaaacacacg ccgccagaaa aacatcgaca
     4261 aggactggac gccgcgggag tgcgcgcagc acggcgacgc tagccatggc tggtcaccaa
     4321 gggcagccgc acgtgcgcat cgagtcgtcc gtttggttcg gccaagcgaa cggtcccacc
     4381 gtgcaaggcg gcccgatcca agacgccgcc gaagccactc gccgaatcga agtcgcttga
     4441 aagcggcccg gtgacgagga tctccagagc gtcggcttcg aacgtgacgc gaacctgcgc
     4501 cctcgctgcc gggtcgtcgt cgagggagga gatgagtcgt tcgacgatcc gatagctcga
     4561 cagctcgatg ctggcaggca gtgcgcgctg atcgccctcg atgctcaggt gcacctcaca
     4621 cgtcgtcgcc gagcgcagga ggtcccgaag ggcatcgagg tcgggctctg gttgggtcga
     4681 tgccgaccgg attgtcccga cgatgtggcg catgcggtcg agggctgccc ggccggtttg
     4741 ttcaatagca gtaaggcttt cggccgtggg tatggtcccg gcacgcgcac cggatgccgt
     4801 ctgcgcgatt tcgtggatct gcagtcgcag gccatcgacg agttcgccgc gaatacgggc
     4861 ctgatcggcg gcgaccgcaa gttccgccgt ccgctcacgt tgttcgatca gctgggcatt
     4921 gcgttcgcgc agctgcgtca ccaataccga gcgggatcgc acgctcatgc cggcgccgaa
     4981 gaacagcaac gtcgccggtg ccatcaatgt gatctgcgac gcaccgagct tcggatccca
     5041 tacgcactga agtgccaccc cggccaggac aagtgtgaga ccgccccaac gctcccggcc
     5101 gatcgtcgcg gcggcgatga cgtacgccac gaagaacgcg gccggcagcg ctggcccaca
     5161 tcgcaccagg tgcccgaaga acagattgtt caggagcgtg cctgctgcca gcaccgtcgc
     5221 cacgatgaac ggcgagcgcc gtgcgaaccc gacagggacg atcattatga gcactgcgac
     5281 gggggcaccg atgcggccgg tgtgctgcga gctgacgaga gccacggccc atgcgctcag
     5341 caacaccgtg aacagcgcag cgccaacgct gacgccgacc actctccggc gcagcaagcc
     5401 gaacgcgttg ttcatcgcca tctcctgccg gtgagccgaa ggcttgataa gcattgtcgg
     5461 tgccgtgggg ccgctgggct actgcgcctg cacgtcggcg gctgcgcgac gaccggatcc
     5521 cagtgcgagc accccagcga tcacaacgac cgccacgatg ccgatcccta tgccaagcac
     5581 accggtccac gggatgtggc cggcgccttg gccgcggtgt gtcttgaagt gcccgacgag
     5641 caggcagcct gcgaccatca cggcggtcgt ggccgacacg ctccacgcaa cgaccttcgc
     5701 gccacgctga ccggagaact gcggccggac catcgacctc ccgccgcgaa tgccgcgccg
     5761 tgcgcgtagg cggtagatgc cgtacgcgac cgcacagatg atcgagatga tgatcacctg
     5821 cacgatccag tagctcacgc tcgcgatggt cgagggcgga ttgctgccaa accctgcacc
     5881 aaggtcccag ctttgcaccg ctgtcgccgc gagcatcgct gcgagcacgg agagcatcag
     5941 gccgcggacg ctgggggcga gcagccctgc gatgactgcg atgacaaggc cgatcagcgg
     6001 agtcattgct cctcctgaag cgtgaatgac gttggtcagg ccagcgtgat cgacgcctcg
     6061 atcgggcaca tccgactgcg ggggtatctt cgcgatgcgc atccacctca ggtcggacag
     6121 ccgcggacgc gcggcaagca agtcgctacc cttgcctgat ccgtctcagg aaggaccgac
     6181 accgtgatgg agcaggcgtc gcccgcggtc tcccgacagg cgcccgcacc gatccgcgtg
     6241 gttatagctg acgaccagcg gctggtccgg acggggtttc gcgtcatcct ggagcgggag
     6301 cccgacatcg aggtcgtcgg cgaagcagac gacggtgtgg cggcgatcca gcagactctc
     6361 gacgtgcagc ccgatgtcgt cctgatggac atccggatgc cacacctcga cggcttccag
     6421 gcaacgcgtc gagtgcttgc cgagacgggc agcaaggtcg tgatcctgac gacattcgac
     6481 tccgacgaat acgtctacga agcgttgcgc agcggggcga gtgggttcct gctcaaggac
     6541 gcgccagctg accagttgat cgctgcggtc aggtgcgccg ctacgggcga tgccctaatc
     6601 gatccggccg tgacgcgacg tctggtcacc agattcgctc acgcgctgcg gccgccgacg
     6661 ggcacccccg aacggttcgc cgcgctgacc gcgcgcgaac tggatatctt gcggctcatc
     6721 gcacgcggcc tgaacaacgc cgagatagcg gcccacctcg tcatcgagga gagcaccgtc
     6781 aagacccacg tcggccggat tcttgcgaag ctcggccttc gcgatcgtgt gcaggccgtc
     6841 gtgctcgcct acgagacggg tctgatcagc cccgacggta gcgccgaatc gggatagggc
     6901 tatttcttca gccagctctc aacacggctg gagggccgtt taccgtgagg gctccgaccg
     6961 acccgaagtc gctcgtcacg tgtccgggta ctgatcgaaa cgatcccatg ggcgacgttt
     7021 ctgcgggaaa ccgccagtga aacgtactgg caggggcgtt atcgcctatc aaagcggcgg
     7081 cgc
//
LOCUS       DATCBJ010000486         2112 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_87294, whole genome shotgun sequence.
ACCESSION   DATCBJ010000486 DATCBJ010000000
VERSION     DATCBJ010000486.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2112)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2112)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2112
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_87294"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(34..315)
                     /locus_tag="VII50_09875"
                     /pseudo
     CDS             complement(34..315)
                     /locus_tag="VII50_09875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611352.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase core domain-containing protein"
     gene            359..1761
                     /locus_tag="VII50_09880"
                     /pseudo
     CDS             359..1761
                     /locus_tag="VII50_09880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012330561.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            1769..2048
                     /locus_tag="VII50_09885"
                     /pseudo
     CDS             1769..2048
                     /locus_tag="VII50_09885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611352.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="integrase core domain-containing protein"
BASE COUNT          365 a          661 c          755 g          331 t
ORIGIN      
        1 cccgttttcg tggacggttg tcggcttggg tgattacgcc ttcatgagca tcttctcctc
       61 gtaagcgatc ggcgatagat agccgagcgc ggaatgccgc cgtgccgggt tgtaccagcc
      121 ctcgatgaag cggaagaccg ccatgcgggc ctcgacctgt gagccgaacc ggcggcgtgc
      181 cagcaattcg cattcaagcg tcgcgaagaa gctttcgcac atggcgttgt cgtacgcgtc
      241 gccgaccgac cctatcgacg gccgcacccc cgcttcccgg cagcggccgc cgaacgcgag
      301 cgaggtgtat tgagtggatt cgagcggtcg tcgcaacacc ttaagggagg gaattgcgat
      361 gacggctcgg aagcggcgct cggatcgtgc ggggcgaggc atgttgaggt cgccggggcg
      421 gccaggggtg gcgcgacgtg aggaccggcg aaggttctgg acagcgatcg cggcagggct
      481 ggccagtgag gatgcggcgg ttggcgcagg ggtgtcgcca gcggtagggg tacgctggct
      541 ccgggaggcg ggtggcatgc caccatcgac gcttgcacca tcgtcgaaac cgttgtccgg
      601 acggtatctg tcgtttgcgg agcgagagga gcttgcgatc ctgcatgcac agggcgcggg
      661 agtgcgggag gtcgcccgac agatagggcg gtcggcatcg acgatctcgc gggagctgcg
      721 gcgcaacgct gcgacgcgca gcggcggcct ggactatcgg gcgaccaccg cgcagtggca
      781 tgccgaacgg accgcccgac gcccgaaacc tgcgaaactg gcggacaacg cggcgctgcg
      841 gacgtatgtg caggatcgat tggccggcgt ggtcgtcact cctggcggga ctgcggttcc
      901 cgggccatcg gtgtcctgga aggggcgccg acacggacct cggcaggatc gtcggtgggc
      961 ctcggcgcgg agccggagca gattgcccat cgcctacggc tcgatttccc ggacgatgag
     1021 acgatgcgca tcagccacga agccatctac cagtcgcttt atgtgcaagg tcggggggct
     1081 ctgcgccgcg agctgaccgc ctgtttgcgc accggacggg cgttgcgcgt gccgcgggca
     1141 cgcacccgcg gacggggcaa atctttcatc gtccccgaga tcatgatcag cgagcgaccg
     1201 gcggaggtgg gtgaccgcgc agtgccgggc cactgggaag gggatctcat cctggggctg
     1261 gagagttccg cgatcggcac cctggtcgag cgcacgacgc ggttcacgat gctgctccac
     1321 ttgcctcgga tggcggacca cggtctggaa gctcgcgtga agaacgggcc ggcgctcgcc
     1381 gggcacggtg ccgaggcggt gcgcgacgcc atcgcgcgca cgatcaccac cctgccggag
     1441 cagctccgtc gatcgctgac ctgggatcag ggagcggagc tggcccagca cgcacggctg
     1501 cggatcgaca cgggcctgcc ggtctacttc tgcgatcccc acagcccgtg gcagcgcggc
     1561 acgaacgaga acaccaacgg gctgctgcgg cagtatttcc cgaagggcac cgacctgagc
     1621 gcgcgcagtg cggacgacct cgcggcggtg gctgcaaccc tcaacgccag gcctcgcaag
     1681 actccggact ggagaacgcc cgccgaggcg cttgatcaag tcctgcgatc ggcgcagacg
     1741 accgttgcga cgaccggttg aactcaagca atacacctcg ctggcgttcg gcggccgttg
     1801 ccgggaagcg ggcgtgcgcc agtccatggg gtcggttggc gacgcctatg acaacgccat
     1861 gtgcgagagc ttctttgcga cgctcgaatg cgaactgctg gcacgccgcc gggttcggct
     1921 cacaggtcga ggcccgcatg gcggtcttcc gcttcatcga gggctggtac aaccaggcac
     1981 ggcggcattc cgcgctcagc tatctatcgc cgatcgctta cgaggagcaa atgctcatga
     2041 aggcgtaatc acccaagccg acaactgtcc acggaaacgg ggcagcttca cagcagcacc
     2101 ggcataagga gt
//
LOCUS       DATCBJ010000487         3153 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_48462, whole genome shotgun sequence.
ACCESSION   DATCBJ010000487 DATCBJ010000000
VERSION     DATCBJ010000487.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 3153)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3153)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3153
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_48462"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..782
                     /locus_tag="VII50_09890"
     CDS             <1..782
                     /locus_tag="VII50_09890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011718856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="class E sortase"
                     /protein_id="HEY5051199.1"
                     /translation="TSSDFVFASASRANQAIRVLGELLITLGVVVFLFIGYELWFTGL
                     YTEHAQSNFKRQLATEWASPAPAVTPGPTSTGSPGTTASASPGATSVPAASVPAIAPI
                     ALPGDAIGIIRIPRLGAKYGYAIVEGVSTDDLKKGPGHYPGTANPGGIGNFVVSGHRT
                     TYLAPFNGIGELAIGDAIVIETRDTWYTYLVTKTQTVLPTDTGVVLPVPGQPRATPTQ
                     ALITLTTCTPKYSASHRLIVTGLLQSALPKSAGTPPALGGS"
     gene            785..1027
                     /locus_tag="VII50_09895"
     CDS             785..1027
                     /locus_tag="VII50_09895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5051200.1"
                     /translation="MYEWLWRKLPGGFAGKLSGCAVLLVGVLALLFFVIFPWVEPRLP
                     WNDVNVNSPSIGQTVPSSSPAPAGSPSVSPSTLPAG"
     gene            1087..1698
                     /locus_tag="VII50_09900"
     CDS             1087..1698
                     /locus_tag="VII50_09900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011718858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HEY5051201.1"
                     /translation="MRILVVDNYDSFVFNLVQYLGQLGATCVVLRNDQVAPADAAGFD
                     GVLLSPGPGIPEDAGVCVPLIQEMAGRVPIFGVCLGHQAIGVAYGAVVNRAPELLHGK
                     TSEVHHAGAGVLVGLPDPFTATRYHSLAVVESTLPDSIEVTGRTDSGVVMAMRHRDLD
                     VEGVQFHPESVLTQGGHRMLANWLVRCGDADAPARAELISSPL"
     gene            complement(1722..>3153)
                     /locus_tag="VII50_09905"
     CDS             complement(1722..>3153)
                     /locus_tag="VII50_09905"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PASTA domain-containing protein"
                     /protein_id="HEY5051202.1"
                     /translation="DLLKTERHIMPTRALEITAEILKALDYSHRNGIIHRDIKPANVM
                     LTRSGEVKVMDFGIARSVAQASAAVTQTAAVIGTAQYLSPEQARGEQVDPRSDVYSTG
                     CLLYELLTGTPPFTGDSAVSIAYQHVREDPIPPSRVNPDVSPAIDAIVLKAMAKNPAN
                     RYQSAAEMREDIERALSGVPVHATPLLHDQTVSIAPVVGEEIGRRQPRGRRPLVYIML
                     GLIACAVFIALGLLLRQVLTTKHPAVTVQVPNVTGFSQADAISTLKTAQLNPVLQLAG
                     SPTVGKGLVISENPPTGTPLPLKTNVTIYVSSGPAAVAVPDLTNQSLADAKAELLRDK
                     LTLGTITPQDSALAANTVLSQSPKAGATIGSGTAVNVVVASGNSTVPDVRNLPLDEAE
                     QALTTNGFNFQQVDQASSSFTPGTVINQVPGHDSLAKTGTVVKLFVAVAPPPPSSSRP
                     ASPPPTSATPTTSATATPTASTTPSP"
BASE COUNT          473 a          995 c         1087 g          598 t
ORIGIN      
        1 cgacgtcatc ggatttcgtg ttcgcgagtg cgtcccgcgc gaaccaggcg atccgggtat
       61 tgggtgagct gctcatcacc ctgggcgtcg tcgtgtttct gttcatcggc tacgagttgt
      121 ggttcaccgg gctgtacacc gaacacgccc agtcgaactt caagcggcag ctggccactg
      181 aatgggcgtc gccggccccg gcggtaacgc ccgggcctac gtcgactggc tcgcccggca
      241 cgaccgcatc ggccagccct ggcgcgacgt cggtgcccgc ggcatcggtg cccgcgattg
      301 caccgattgc cctgccaggg gacgcgattg gcatcatcag gatcccgcgg ctcggtgcca
      361 agtacggcta cgcgatcgtc gagggggtgt cgaccgatga cctcaagaaa ggccctgggc
      421 actacccggg cactgccaac ccaggaggta tcggcaactt cgtggtgtcc ggtcaccgca
      481 ccacctacct cgcgccgttc aacggcatag gcgagctggc gattggcgac gcgatcgtca
      541 tcgagacccg tgacacttgg tacacctacc tggtcacgaa gacgcagacg gtgctgccga
      601 ccgacaccgg ggtggtgcta cctgtgccgg gccagcctcg cgcgacgccg acccaagcgc
      661 tgatcacgtt gaccacctgc acaccgaaat actcggcgtc gcaccgtctc attgtcaccg
      721 gcctgctcca gtcggcacta cccaagtcgg ccggaacgcc gccggcgctc ggggggagtt
      781 agccatgtac gagtggttat ggcgcaagct gccgggcggc ttcgccggca agctcagcgg
      841 ctgtgccgtg ttgctcgtcg gcgtcctcgc cttgctgttc ttcgtgatct tcccgtgggt
      901 cgagccacga ctgccgtgga acgacgtcaa cgtgaactcg ccgtcgatcg gacagaccgt
      961 tccctcgtcg tctccggcac cggccgggtc gccgtcggtg tcgccgtcca cgctgcctgc
     1021 tggctaggcg gctcagccag agatagtccg gggacgtggg acgccggcat ctgggaggat
     1081 cgccgggtgc gcattctggt cgtcgacaac tacgacagct tcgtcttcaa cctcgtccag
     1141 tatctgggcc agcttggtgc gacctgcgtg gtgctgcgca acgaccaggt cgcacctgcc
     1201 gatgctgccg ggttcgacgg cgtgttgctg agcccggggc ccgggattcc cgaggacgca
     1261 ggggtctgcg taccactcat ccaggagatg gccgggcggg tgccgatttt cggtgtgtgc
     1321 ttggggcatc aggccatcgg ggttgcgtac ggcgcggtcg tcaatcgcgc cccggagttg
     1381 ctgcacggga aaaccagcga ggtgcatcac gctggcgccg gtgtgctggt cgggctgccc
     1441 gacccgttca ccgcgacccg ctatcactca ctcgccgtcg tcgagtccac gctgccggac
     1501 agcatcgagg tgacgggtcg aaccgactcg ggcgtcgtga tggccatgcg gcatcgcgac
     1561 cttgacgttg agggcgtcca gtttcaccct gagtcggtcc tgacgcaggg cggtcaccgg
     1621 atgctggcca actggttggt gcgctgcggt gacgccgacg ctccggcccg cgccgaactg
     1681 atcagttcgc cactgtaagc agctgaccaa cgagctaacc gctacggcga cggtgttgtg
     1741 cttgcagtcg gtgtcgccgt cgcgctcgta gtcggcgtcg cactggtcgg tggtggtgag
     1801 gcaggccgtg aggacgacgg cggtggcggt gcgacggcca cgaacagctt gacgacggtg
     1861 ccggttttcg ccagcgagtc gtgcccgggt acttggttga tgaccgtgcc tggagtaaac
     1921 gagctactcg cctggtcgac ctgttggaag ttgaagccgt tcgtggtcag cgcctgttcg
     1981 gcttcgtcga gcggcaggtt gcggacatcg ggcactgtgc tgttgccgct ggcgactacc
     2041 acgttcaccg cggtgcccga cccgatggtc gcgcccgcct tggggctttg gctcagcacc
     2101 gtatttgcgg ccaatgcgga atcctgcggc gtgatagtcc cgagggtcag cttgtcgcgc
     2161 agcagttccg ccttcgcatc ggccagtgac tgattggtga ggtcggggac cgcgaccgca
     2221 gcagggccgc tcgagacgta gatggtgacg tttgtcttca gcggaagcgg cgtgccggtc
     2281 ggcgggtttt cgctgatgac gaggcccttg cctaccgtcg ggctaccggc cagctgtagg
     2341 accggattca gctgcgcggt cttcagcgtc gagatcgcat cagcttggga gaacccggtc
     2401 acattcggaa cctgcaccgt caccgcgggg tgcttggtcg tgaggacttg acgaagcagc
     2461 agaccgagag cgatgaaaac ggcgcaggcg atgagcccga gcatgatgta cacgagcggc
     2521 cgacggccgc gcggctgccg ccgaccgatc tcctcgccga cgacgggcgc gatgctgacg
     2581 gtctggtcgt gtagcagcgg agtggcgtgg accggcacgc cgcttagcgc gcgctcgatg
     2641 tcttcgcgca tctccgcggc actctggtag cggttcgccg gattcttggc cattgccttg
     2701 agcacgatcg cgtcgattgc cgggctcacg tcgggattga cccgcgacgg cggaatcggg
     2761 tcttcgcgca catgctggta ggcgatggac accgccgagt caccggtgaa cggcggcgtc
     2821 ccggtcagca gttcgtaaag caggcagccg gtggagtaca cgtcgctgcg tgggtcaact
     2881 tgctcgccgc gcgcttgttc gggcgacagg tactgcgcgg tgccgatgac ggcggccgtc
     2941 tgcgtcactg cggcgctcgc ttgcgccacg gatctcgcaa taccgaagtc catgaccttg
     3001 acctcgcccg accgcgtcag catgacgttg gcaggcttga tgtcgcgatg gatgatgccg
     3061 ttgcggtggc tgtagtcgag tgccttgagg atctccgcgg tgatctcgag tgcgcgagtc
     3121 ggcatgatgt gccgctcggt cttcaggagg tcg
//
LOCUS       DATCBJ010000488         5838 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_93268, whole genome shotgun sequence.
ACCESSION   DATCBJ010000488 DATCBJ010000000
VERSION     DATCBJ010000488.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 5838)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5838)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5838
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_93268"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            180..2264
                     /gene="glgX"
                     /locus_tag="VII50_09910"
     CDS             180..2264
                     /gene="glgX"
                     /locus_tag="VII50_09910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004133 - glycogen debranching enzyme
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HEY5051203.1"
                     /translation="MDIWPGHWQPLGATVDDGGTNFALFSANATGVDLCLFDDLGTET
                     RVPLRESTFHVWHGYVPGVGVGTRYGYRVDGELNPSDGSHFNESKLLADPYARAFDGA
                     FELGDAVFENDPHDSAAFVPKCVVVRDEFDWGKDRSPAVAWPDTVVYEVHVRGFTMQH
                     PDVPPELRGTYAGLAHPAATDYLKQLGVTTIELLPVHHFVSEPALLRRGLSNYWGYNT
                     LGFFAPHGSYSSSGTRGEQVREFKEMVKALHAAGLEIILDVVYNHSAEGDELGPTLSL
                     RGIDNQSYYRLEPGGLYVNYTGCGNTLDLRSPHMLQLVTDSLRYRVTEMHVDGFRFDL
                     APTLARSSDAFDAQSSFLEAINQDPVLSQVKLIAEPWDVGPGGYQVGKFPAPWTEWND
                     RFRDTIRDFWLCGATDARELGYRLSGSSDLYQDSGRRPDASLNFVTCHDGFTLHDLVT
                     YNEKVNDANGESNRDGSSDNHNWNCGFEGETDEPVVTQIRNRVTRAMLGTLVLSAGVP
                     MLGHGDEIGRTQRGNNNAYCQDNALAWVDWSDRDDSLLAFTQAAIALRRGHAVFRQSS
                     FFTGTGVRDVASSAVSAVGVKDLAWFAADGVEMTPPDWPDLRARTIGMYLSGNDIRDV
                     GPHGEPIVDDSFLIVIHAGLEPSSFTLPGAPWATNYEVVLDNTGPTSSATLAGGSILD
                     VPAVAFIVLRAT"
     gene            complement(2277..4919)
                     /gene="glgP"
                     /locus_tag="VII50_09915"
     CDS             complement(2277..4919)
                     /gene="glgP"
                     /locus_tag="VII50_09915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucan family phosphorylase"
                     /protein_id="HEY5051204.1"
                     /translation="MDLGGTITGFGMVVPVRAIRRFTVRTVLPSRLAALGDLVMNLRW
                     SWHAETLDLFESIDPQTWHAVDGDPGRLLGLVPHSRFTELSTDEQFLGRLDEAAENLR
                     QYLNQDRWFQTLGDEAPKAIAYFSPEYGITAVLPQYSGGLGILAGDHLKTASDLGVPV
                     IGVGLLYRRGYFNQSLSRDGWQQERYPPVDPHGLPLTLLTEADGSPVTIPVRLPGRDL
                     QAQVWLCQVGRIPLLLLDSDVEGNAPAERDVTDRLYGGGTDHRFRQELLLGVGGIRAV
                     RAFCRLTGHAKPEVYHMNEGHAGFLALERIRELVTASSLSFDEALEVVRASTLFTTHT
                     PVPAGIDRFPRELVEHHFGPPDNALGLPIDRVLALGSESFPGGDQSVFNMALMGLRVA
                     QRANGVSLLHGKVSREMFAGLWPKFDPADVPITSVTNGVHAPTWVSREVQQMVERELG
                     VDLQADGAAWAGADHLDDRQLWEVRRALRTRLVDETRVRLRASWIQRGAMASELGWIG
                     NVLDPDVLTIGFARRVPSYKRLTLMLQDRERFTELLLDKDRPVQFVIAGKAHPADDGG
                     KRLIQEVVRFADAANVRHRVVFLPDYDMALARVLCAGADVWLNNPLRPLEACGTSGMK
                     AALNGVLNLSIRDGWWDEWSDGSNGWDIPSADELTEERRDELEAAAFYDIVSTQMVPR
                     FYDHDHNGLPSRWLEMVRHTLSSLGPKVVATRMLRDYVNNLYTPAAQSSRAMEDTKFG
                     GAKHLAAWKKRVASAWPGVRVEHIEARGVSDALEYGTVVSLRAVVGLGDLAADDVDVQ
                     VASGRVDENDQITGPAYSSLRLSDKRDDGSWVYEGEMTLDRTGPFGYSVRVLPADALL
                     ASPGELGLIAIPPADEGMVTGDLR"
     gene            complement(5044..5289)
                     /locus_tag="VII50_09920"
     CDS             complement(5044..5289)
                     /locus_tag="VII50_09920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5051205.1"
                     /translation="MTFSIGIRPLVGVPACAVAVSVVFVAFAGAARAERAVRVAGVAA
                     FERAGRVAGVFGGVARLLFVLITDERIGRRVAPREAG"
     gene            5288..>5838
                     /locus_tag="VII50_09925"
     CDS             5288..>5838
                     /locus_tag="VII50_09925"
                     /inference="COORDINATES: protein motif:HMM:NF023324.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltotransferase domain-containing protein"
                     /protein_id="HEY5051206.1"
                     /translation="MPVIDCGRYPAKSVVGEQLTVAATAFREGHDALGVNVVLRDSTG
                     RTGPWTPMRLVAPGTDRYEAVITADTPGPWTFQIEAWGDPLESWRHDARIKIPESQDV
                     ELMLEEGARLHERAAANLSDGDGGRLMGVAETLRSPGDPVERLSAALARGVLALLARH
                     PLRTLLTVSPSYPLWVDRERALFG"
BASE COUNT         1079 a         2005 c         1807 g          947 t
ORIGIN      
        1 cgtggtcgga cccgcccatg tacacggctt gctgcagcgc gtcggactca cgaccggtga
       61 cgcgtggatt atcgcctcgg cgatctggat gatgcgcgac gcgacattgg cgcctgacaa
      121 cgacacgaca tctcgacccg ctgcgtcgga gatcgagcct gacccggcag gataagccgg
      181 tggacatctg gcccggtcat tggcagccgc ttggtgccac cgtcgatgac ggtggcacca
      241 acttcgcgct cttctccgcg aacgccaccg gggttgactt gtgcctgttc gacgacttgg
      301 gcaccgagac ccgtgtcccg ctgcgcgagt caacgtttca cgtctggcac ggctacgtcc
      361 caggcgtcgg ggtcggcacc cgctacggat accgcgtcga cggcgaactc aacccgagcg
      421 acggctcgca cttcaacgaa agcaagttgc tcgccgaccc gtatgcccgc gcgttcgacg
      481 gcgctttcga gctgggcgat gcagtcttcg agaacgaccc gcacgactcg gccgcattcg
      541 tgccgaagtg cgtcgtcgtg cgcgacgagt tcgactgggg taaggatcgg tcacccgccg
      601 tcgcctggcc cgacacggtc gtctacgagg tgcacgtccg cggcttcacc atgcaacacc
      661 ccgacgtacc acccgaattg cgcggcacgt atgccgggct cgcgcaccca gccgcgaccg
      721 actacctaaa gcagctcggc gtcaccacta tcgagttgct gcccgtgcac cactttgtga
      781 gcgagccggc gctgcttcgc cgcggcttgt cgaattactg gggctacaac acgctcggct
      841 tcttcgcacc acacggcagc tattcgtcgt ccggcactcg gggtgaacag gttcgagagt
      901 tcaaggaaat ggtgaaagcg ctgcacgccg ccgggcttga aatcattctc gacgtcgtct
      961 acaaccacag cgccgagggc gacgagttag gcccgacatt gtcattgcgt ggaatcgaca
     1021 accagtcgta ttaccggctc gagcctggtg gactgtacgt gaactacacc ggctgcggta
     1081 acacgctcga cctgcgtagc ccgcacatgt tgcaactcgt cacggactcg ctgcgctacc
     1141 gggtgacaga gatgcacgtc gacgggttcc gtttcgacct cgcgcccacg ctggcccgct
     1201 caagcgatgc attcgacgcg cagtcgtcgt tcctggaggc gatcaaccag gaccccgtgc
     1261 tctcgcaggt caagctgatc gccgagccgt gggatgtcgg gccaggtggt taccaggtcg
     1321 gcaaattccc cgcaccgtgg accgagtgga acgaccgctt tcgcgacact atccgtgact
     1381 tctggctctg cggcgcaacc gacgcacgcg aactcggcta ccgattgtcg gggtcctcgg
     1441 acctctatca ggacagcggg aggcgccccg atgcatcact caacttcgtc acctgtcacg
     1501 acggcttcac gttgcacgac ctggtgacct acaacgagaa ggtcaacgat gcgaacggcg
     1561 agagtaaccg agacggctcc agtgacaacc acaactggaa ctgtgggttc gagggcgaga
     1621 ccgacgaacc ggtcgtcaca caaatccgca accgcgtcac gcgggcgatg ctcggaacgc
     1681 tggtgctgtc ggccggtgtg ccaatgcttg ggcacggcga cgagattggc cgtacccagc
     1741 gcggcaacaa caacgcctac tgccaggaca acgcactcgc gtgggtcgac tggagtgacc
     1801 gcgacgactc gcttctcgct ttcacccagg ccgcgatcgc tctgcgcaga ggacatgcag
     1861 tgtttcgcca aagctcgttc ttcacgggca cgggggtgcg cgacgtcgca agcagcgctg
     1921 ttagtgccgt aggggtcaaa gatctcgcgt ggttcgccgc agacggtgtg gagatgacac
     1981 ctccggactg gcccgacctg cgcgcgcgca cgatcgggat gtacctgtcc ggcaacgaca
     2041 tccgcgacgt cggcccgcac ggcgagccca tcgtcgacga ctcgtttctc atcgtcatac
     2101 acgccggtct cgaaccatcg tcgttcacac tcccgggtgc accttgggcg acgaactacg
     2161 aggtggtgct cgacaacacc ggcccgacga gcagcgccac ccttgccggg ggcagcatcc
     2221 tcgacgtccc cgccgttgcc ttcatagtgc ttcgcgccac ctgacccgcg ggaaagctag
     2281 cggaggtcgc cggtgaccat tccttcgtcg gcgggcggga tggcgatcag tccgagttct
     2341 cctggcgagg ccaacaaggc gtcagcagga aggactcgca cgctgtaccc gaacggtccg
     2401 gtgcggtcga gcgtcatctc gccctcgtag acccaactgc cgtcgtcgcg cttgtccgaa
     2461 aggcgcagcg atgagtacgc cgggccggtg atctggtcgt tctcgtcgac gcgacctgac
     2521 gcgacctgca cgtcgacgtc gtccgctgcg aggtctccca acccaaccac cgcgcgcagc
     2581 gataccaccg tgccgtactc gagcgcgtcg gagacgccac gagcttcgat gtgctcgacc
     2641 cgcacgcccg gccaggctga cgcgacccgc ttcttccagg ccgcgaggtg tttcgcgccg
     2701 ccgaacttcg tgtcctccat agcccgcgac gactgcgcag ccggggtgta caggttgttc
     2761 acatagtcgc gcagcatgcg ggtcgcgacc accttcgggc caagcgacga gagcgtatga
     2821 cgaaccatct ccagccaccg gctcggcagc ccgttgtggt cgtggtcata gaagcgcggc
     2881 accatctggg tcgacacgat gtcgtagaac gcggccgcct caagttcgtc gcggcgctcc
     2941 tcggtcagct cgtccgccga cgggatatcc cacccgttgg agccgtcgga ccactcgtcc
     3001 caccagccat cgcgaatcga caggttcagg acgccgttca atgcggcctt catgcccgac
     3061 gtcccgcatg cctccagcgg gcgcagcggg ttgttcaacc agacgtcggc gccggcgcac
     3121 aacacccggg ccaacgccat gtcgtagtcg ggcaagaaca cgacccgatg ccgcacgttc
     3181 gcggcatcgg caaacctcac aacctcctgg atcagccgct tcccgccgtc gtcggccggg
     3241 tgcgccttgc ccgcgatgac gaactgcacc gggcggtcct tgtccagcag cagctcagta
     3301 aatcgctcac ggtcttgcag catcaacgtc agccgcttgt acgacggcac gcgacgggcg
     3361 aacccgatcg tcaagacatc ggggtcgagg acgttgccga tccagccgag ctcggaggcc
     3421 atcgcaccgc gctggatcca cgacgcgcgt agccgcacgc gcgtctcgtc gaccaaccgg
     3481 gtgcgcagcg ctcggcgcac ttcccacaac tggcggtcgt cgaggtgatc ggcacccgcc
     3541 cacgcggcgc cgtcggcctg caggtcgacc cccagctcac gctcaaccat ctgctgcacc
     3601 tcgcgtgata cccacgtggg cgcgtgcacg ccgttggtca ccgaagtgat cgggacatcg
     3661 gccgggtcga acttcggcca cagcccggcg aacatctccc ggctgacctt gccatgtaac
     3721 aacgaaacgc cgttggcgcg ctgtgcaacg cgaaggccca tcaacgccat gttgaagacg
     3781 ctctggtcac caccggggaa gctctccgag ccaagcgcga gcacgcggtc gatcggcagc
     3841 ccgagcgcat tgtcgggagg gccgaagtgg tgctcgacca actcgcgcgg gaacctgtcg
     3901 atgccggccg gcaccggcgt gtgcgtagtg aacagcgtcg acgcgcgaac gacctcgagc
     3961 gcttcgtcaa acgacaacga cgaggcggtc accagctcgc gaattcgctc cagcgcaagg
     4021 aagccggcgt gcccctcgtt catgtggtaa acctcgggct tcgcgtgccc ggtcagccga
     4081 caaaatgcgc gtaccgccct gatcccaccg acgccgagca gcagttcctg gcggaagcgg
     4141 tggtcggtgc cgccgccgta gaggcggtcg gttacgtcac gctccgccgg cgcattgccc
     4201 tcgacgtcgg aatccagcag cagcaacggg atgcggccga cctggcacaa ccagacctga
     4261 gcctgcaagt ctcgaccggg caaccggacc gggatcgtca ccggcgagcc gtcggcctcg
     4321 gtcagcaggg tcagcggcaa gccgtgcggg tcgacgggtg ggtagcgctc ttgttgccag
     4381 ccgtcgcgcg acaacgactg attgaagtag ccgcggcgat agagcaaccc gacaccgatc
     4441 accgggacgc cgaggtcgct cgccgtcttc aggtggtcgc ccgccaaaat cccgagcccg
     4501 cctgagtatt gcggcagaac cgccgtgatc ccgtactcag gagagaagta ggcgatcgcc
     4561 ttcggagcct cgtcgcccag tgtctggaac cagcggtcct gattcagata ctgacgcagg
     4621 ttctcggccg cttcgtcgag ccgaccgagg aactgctcgt ccgtcgacaa ctcggtgaaa
     4681 cgcgaatgcg gaacaagccc gagcaaccgc cccggatccc catcaaccgc gtgccaggtc
     4741 tgcgggtcga tggactcgaa aaggtcgagc gtctccgcat gccatgacca acgaaggttc
     4801 atgacgagat cgccaagagc cgccaagcgc gacggcaaga cagtgcgcac agtgaatcgt
     4861 ctgatcgctc tcacgggcac gaccataccg aagccggtga tcgttccacc aaggtccatc
     4921 ggcactgctg ccacatcgac atagtgggag cgcttgcaat ttcattggcg cattcccaga
     4981 acacaccggc cagtcgaagg catcgtgcag gaaatgcttc ggccacagcg gccgcgagga
     5041 agatcacccg gcctctcgcg gcgcgacccg ccgcccgata cgttcgtcgg taatgagcac
     5101 aaacaagagc cgggccaccc ccccgaaaac gccggccacc cgtccggccc gctcgaaagc
     5161 ggctacgccc gcaacacgga cggcccgctc tgctctcgcc gcgcctgcaa acgcaacaaa
     5221 aacaactgaa accgcaacag cacaggccgg gacgccgacg agcgggcgaa ttccgatcga
     5281 gaacgtcatg cccgtcatcg actgcggccg ttatccggcc aagtcggttg tgggtgagca
     5341 actaacggtc gccgcaaccg cttttcgcga aggccatgat gccctaggcg tgaatgtcgt
     5401 gctccgggat tcgaccggtc gcaccggccc ctggaccccg atgcgactcg tcgcacccgg
     5461 taccgaccgc tacgaagcag tcatcacggc ggacacgccg ggaccgtgga ccttccagat
     5521 cgaggcgtgg ggtgatccgc tcgaatcgtg gcggcacgac gcgcgcatca agattcccga
     5581 gagtcaagac gtcgagttga tgctcgagga aggtgcgcgg ttgcacgagc gggccgccgc
     5641 gaacctctcg gacggcgatg gtggccgact catgggcgtt gccgagacgt tgcgctcacc
     5701 aggcgatccc gtcgagcgat tgtcagcggc actggctcgc ggcgtcctcg ctctgcttgc
     5761 acgccacccg ctgcgcacgc tgctgaccgt gtcgccgtcc tacccattgt gggtcgatcg
     5821 tgagcgcgcg ctcttcgg
//
LOCUS       DATCBJ010000489         5616 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_127528, whole genome shotgun sequence.
ACCESSION   DATCBJ010000489 DATCBJ010000000
VERSION     DATCBJ010000489.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 5616)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5616)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5616
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_127528"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..417
                     /locus_tag="VII50_09930"
     CDS             <1..417
                     /locus_tag="VII50_09930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5051207.1"
                     /translation="RTPEHIINRIRGYGFSQKVAVEFDDRPLERDEKPEDAVIKKNVV
                     DYSTDELRKLVDSRPPQLELPNDRELLTEFQGQEIQYTRDEGSAAGITRRYGGVGGSF
                     HTLDAAKMMIAGRNLDAITQTLKGAPQTPVLDRFGI"
     gene            809..1333
                     /locus_tag="VII50_09935"
     CDS             809..1333
                     /locus_tag="VII50_09935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5051208.1"
                     /translation="MQSVVESAGRSQLRSVPELGSVVEVQILDMTGEPLPEPVAPAPA
                     SNHDTTTPVLVPPMPPPVGAPGGLYIAPMGMGLGTRIQIEAELDGMAADIRLFWAYEP
                     DLVLRKCSAYTARLTELYVALNRAESADRQYTRVRTQQVAIWLEELDRQFKSASRLIE
                     VNRQDVSMLRGHEG"
     gene            1336..3276
                     /locus_tag="VII50_09940"
     CDS             1336..3276
                     /locus_tag="VII50_09940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5051209.1"
                     /translation="MTQQLTQREQGSLVELTPEDLARGHLPDGEPLEGWINASGMPSS
                     SVRGSVERLGRELSMWIEGQRNAALNRSSMFDRYAYTAPDNPYKQMEIARNAVANDDI
                     VGGACDVTEGLIFQGLKWESTSADDSDIFNQLSEDLNLDEIARVMHRELFTYSTAIIG
                     EWWGPRSYKVKGVTRNVNLVPVSVDNMVTGEMEVVVSDMTQNSQVKLAGSPTTKSSPR
                     ARRKTYDIFCPVAYTFLDPLKVVPVGSLMFGMEQLAWYCSKEEMVTWDRIAEGDVIDA
                     IMTEFFVGKYVPSILEKAQISALGIDPNRLMLLNPERVWRICRTRPDYRRFADVRLKS
                     VFRQLDLKQQLMEADRVQLIGAANYILLIKKGDEKFPAIQPEIDNLKDSMKVLARLPV
                     IVSDHRLNIEIITPKQDMTLNHDKYDVLDRRILSRALGSLQVAASGQRAENSLTMSHT
                     VGRLLENQRSSIKRHLERRITHAVVVHTRNANKFSTEPDLQFVPPHIQLDNDAQITQS
                     ILELRQMNELSRDSTLEYFGFDQAVEAQRRESEEQDGYDAIFKSQVPFGSPNAGMAAG
                     AGTAPGAVKPAPSGSDAAGLANQTTGAVGKGATPAKRAATAGPNGAGRTPASSGRSGG
                     RPIGGGTTTKSTARVAPKPAGS"
     gene            3278..4135
                     /locus_tag="VII50_09945"
     CDS             3278..4135
                     /locus_tag="VII50_09945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5051210.1"
                     /translation="MTSLTEIRDGVLRDMPRKSGLDARETTQTGKTDEQPTVARELAL
                     VPGLVIEHPSGKTTLTVPAQRIMAAAEAEKWLLRIQGRFVGSEAPNRNGAMWSADDLM
                     YGLPTVASGPLNWLHSERKVVGCIASAGYVDRQTAAGVVTAPHLQAQSVMWRWLYPEE
                     CRIAERAADNNALWYSMECVSENVECSPTAGGCGAKFPYATYMRERAKTCEHMMSGSA
                     PRRFENPIFQGGAIIVPPIRPGWADADAEVVRTAAAAADGLDAADFSLETSDLTAMLQ
                     QVASWQKAG"
     gene            4145..4537
                     /locus_tag="VII50_09950"
     CDS             4145..4537
                     /locus_tag="VII50_09950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5051211.1"
                     /translation="MSRKTDLAWAAGLFEGEGCVSFASCSVRLVLQMTDRDVVERFAA
                     VVGDGKITVQIPKNERYLPLYRWTKAGAVFARGLLEEWRPYLGERRLARFEEVLAIAD
                     RRHVEQRARRRVRSGLVEIRRAVRADGR"
     gene            4627..5202
                     /locus_tag="VII50_09955"
     CDS             4627..5202
                     /locus_tag="VII50_09955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5051212.1"
                     /translation="MSQTATEPRTFSETELAALVADRVTRQTAEATKERDEAVARATA
                     AENKLDAAVLAQKAAEDKAKAAEEALETQKLEAEKAAESATKKDARIKQIREVAKHIT
                     DETFFTDEARVKRVIAMDDDAFTGYLDDLKRAAGNATKDGGSTPATPPRETAMAGDVA
                     NVTTPTGKATASAAFLGRRFGAAEAASTTTT"
     gene            5219..>5616
                     /locus_tag="VII50_09960"
     CDS             5219..>5616
                     /locus_tag="VII50_09960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5051213.1"
                     /translation="MADDYGLNFGFRRSGESMSVREGRLSVPAGGPVWPAAGSFHQGD
                     FVTIDPASPGFLKLAPANTPAQTGVSGLLVQEDPNLFDSIYNRIGPGILHDSYDLGRI
                     INNRLAIIWAGAGVKFWVKNTLDVTRPDGS"
BASE COUNT         1081 a         1754 c         1861 g          920 t
ORIGIN      
        1 cggacgcctg agcacatcat caaccgcatc cgcggctacg gcttctccca gaaggtcgcg
       61 gtcgagttcg acgaccggcc gctggagcgc gacgagaagc ccgaggacgc ggtgatcaag
      121 aagaacgtcg tcgactactc caccgacgag ctacgcaagc tggtggactc ccggccgccg
      181 cagcttgagc tgcccaacga ccgtgagctg ctcaccgagt tccagggcca ggagatccag
      241 tacacccgcg acgagggctc cgcagcgggc atcacgcggc gctacggcgg cgttggcggc
      301 agcttccaca ccctggacgc cgcgaagatg atgatcgccg gccgcaacct cgacgcgatc
      361 acccagacgc tcaagggcgc gccgcaaacg ccggtgcttg accggttcgg gatctaggtc
      421 taggttccct ctcgtactac ctgtcgcaac gagatcgagg gtggctgcac cgggtaaaaa
      481 ccggggttgc tatgcccgcc tgatttcgga gaaggtatcg gggagtgtcg aagcgataga
      541 tgcccctgcg aaggcccctg gacgcgctgg tagtcaagat ttaggcaggg agccgggaaa
      601 acctaccgac cagcattgaa ggcccggaga cgcctgcgag ggtggctccg ggccttcgtc
      661 gtgcgcgcgc tccgctcagt ccatagtcca tggtctatgg accattgact acccggttta
      721 ctttgtttac ccggcgtgtg agcgcctaag tggcagctcc ggtgggttta ctggcaccta
      781 agtcgagcta actgccgata ggagtgccat gcaaagcgtt gttgagagcg ccggacgcag
      841 ccagctccgc tccgtgccgg aactggggag cgtcgtcgag gtccagatcc tggacatgac
      901 cggcgagccg cttcctgagc cggtggctcc ggcgccagcc tcaaatcacg acacgacaac
      961 gcccgttctc gtgccgccga tgcctccacc ggtcggtgct ccaggaggtc tctacatcgc
     1021 cccgatgggt atggggctcg ggactcgcat tcagatcgaa gctgagctgg acggcatggc
     1081 tgcggacatc cggctgttct gggcctacga gcctgacctg gtgctgcgca agtgttcggc
     1141 gtacaccgcg cggttgaccg agctgtacgt ggctctcaac cgcgctgagt cggccgaccg
     1201 gcagtacacc cgcgtgcgca cccagcaggt cgccatctgg ctcgaagagt tagaccggca
     1261 gtttaagagc gcgtctcggc tgatcgaagt gaatcggcag gacgtgtcaa tgctgagagg
     1321 acacgaaggc tgatggtgac gcagcagctc acgcagcgcg agcagggctc actcgttgag
     1381 ctgacgccgg aggatcttgc acgtgggcac ctgccggatg gcgagccgct ggaaggctgg
     1441 atcaacgcca gtggcatgcc atcgagttcc gtgcgtggct ccgtcgagcg ccttggtcgt
     1501 gagctgtcga tgtggatcga ggggcagcgc aacgccgcac tgaaccgcag ctcgatgttt
     1561 gaccgctatg cctacaccgc gccggataac ccttacaagc aaatggaaat cgcccgcaac
     1621 gcagtggcca acgacgacat cgtcggcggc gcctgcgacg tcaccgaggg cctgatcttc
     1681 cagggactga agtgggaatc cacgagcgcc gacgactccg acatcttcaa ccagctcagt
     1741 gaagatctga acctggatga gatcgcgcgg gtgatgcacc gcgagctgtt cacctactcc
     1801 actgcgatca tcggtgagtg gtggggaccg cgctcgtaca aggtcaaggg cgtcacccgc
     1861 aacgtcaacc tggtgcccgt ttcggtcgac aacatggtga ccggcgagat ggaagtcgtc
     1921 gtctccgaca tgacgcagaa ctcccaggtg aagctcgccg gtagcccgac gaccaagtcg
     1981 tctccgcgtg cgcggcgcaa gacctacgac atcttttgcc ctgtggcata tacgtttctg
     2041 gacccactga aggtggtgcc ggtcggatcg ctgatgttcg gcatggagca gctcgcgtgg
     2101 tactgctcca aggaggagat ggtcacgtgg gaccgcatcg ccgagggcga tgtcatcgac
     2161 gccatcatga ccgagttctt cgtcggcaag tacgtgccgt cgatcctgga gaaggcgcag
     2221 atctccgcac tgggcatcga cccgaaccgg ctgatgctgc tgaatcccga gcgggtgtgg
     2281 cggatctgtc gtacacgacc ggactaccgg cgcttcgccg atgtgcgact gaagagcgtg
     2341 ttccggcagc tcgaccttaa gcagcagctc atggaagctg accgcgtcca gctcatcggc
     2401 gcggctaact acatcctgct gatcaagaag ggtgacgaga agtttcctgc aatacaacca
     2461 gaaatcgaca acctcaagga ctcgatgaag gtgctcgcgc ggctaccggt catcgtgtcc
     2521 gaccaccggt tgaacatcga gatcatcacg cccaagcagg acatgacgct caaccacgac
     2581 aagtacgacg tgcttgatcg gcgtattctt tcccgcgcgt tgggcagctt gcaggtggcg
     2641 gcgtctggtc agcgcgcgga gaactcactg accatgtccc acacggtcgg tcgactgctg
     2701 gaaaaccagc gcagctcgat caagcgacac ctggagcggc gcatcacgca cgccgtggtg
     2761 gtgcacacca ggaacgccaa caagttctcc accgagcccg acctgcagtt cgtgccgccg
     2821 cacattcagc tcgacaacga cgcgcagatc actcagtcga ttctcgagct gcgtcagatg
     2881 aacgaactgt ctcgcgactc gacgctggag tacttcggct ttgaccaggc agtggaggcg
     2941 cagcgtcgcg agagcgagga gcaggacggc tacgacgcca tcttcaagtc ccaggtgccg
     3001 tttggttcgc cgaacgccgg catggccgcg ggcgccggca cagcgcctgg tgcggtcaag
     3061 cccgctccga gcgggtcgga cgcggctggg ctggcgaatc agacgactgg ggcggtcggt
     3121 aagggtgcca cgccggccaa gcgggcggcc acggccggcc cgaacggtgc cggccgcacg
     3181 ccggcttctt ccggtcgcag cggtgggcgg ccgataggtg gagggacgac cacgaagtcg
     3241 accgcacgag ttgcgccgaa gcctgctggg agctgacatg acgagcctga ccgagatccg
     3301 cgacggtgtt ttgcgcgata tgccgcgtaa gtccggcttg gatgcacgtg aaacaacgca
     3361 gacgggcaag acggacgagc agcccaccgt ggcgcgcgag ctggcgctgg tgccagggct
     3421 ggtcatcgag cacccatccg gcaagaccac gctcaccgtg cccgcacagc gcatcatggc
     3481 tgccgctgag gccgagaagt ggctgctgcg catccagggc cgcttcgtgg gctccgaggc
     3541 gcccaaccgc aacggggcga tgtggagcgc ggacgacctg atgtacgggc tgccgaccgt
     3601 ggccagcggc ccgctgaact ggctgcactc cgagcgcaag gtcgtcggtt gcatcgccag
     3661 cgccggctat gtggatcgcc agacggcggc cggcgtggtc accgctccgc atctccaggc
     3721 gcagtcggtg atgtggcgct ggctgtaccc cgaggagtgc cgaatcgccg agcgcgcggc
     3781 cgacaacaac gcgctgtggt actcgatgga gtgcgtgtcc gagaacgtcg aatgctcccc
     3841 caccgcaggc ggttgcggcg cgaagttccc ctacgcgacc tacatgcgcg agcgcgccaa
     3901 gacgtgcgag cacatgatga gcgggtccgc cccgcgccgg ttcgagaatc cgatcttcca
     3961 gggtggcgcg atcatcgtgc cgccgatccg gcctggttgg gcggacgccg acgccgaggt
     4021 cgtgcgtacg gcggcagcgg cagcggacgg gctcgacgcg gctgacttca gcctggagac
     4081 cagcgatttg accgccatgc tccagcaggt tgcgtcctgg cagaaggctg gctgagcccg
     4141 agacatgagt cgtaagaccg acctcgcatg ggctgctggt ctgttcgagg gtgaaggctg
     4201 cgtttcgttt gctagctgtt ccgttcggtt ggtccttcag atgaccgatc gagacgtggt
     4261 tgagcggttt gctgccgtcg tgggtgacgg caagatcacc gtgcagatcc cgaagaatga
     4321 gcgttacctg ccgttgtacc gctggaccaa ggcgggtgca gtcttcgctc gcgggctact
     4381 tgaggagtgg cgcccgtacc tgggtgaacg tcgactggcg aggtttgagg aggttctggc
     4441 catcgctgat cgacggcatg ttgagcagcg agcgcgacga agggtccgtt cgggattggt
     4501 cgaaatccgc cgagctgtgc gcgcggacgg gcgttagcgt ccgttagcgt cacattgtgt
     4561 cgatggtccc cacgaggccg gtcgaggcaa gtctcgacct catcactcgt agggaggtaa
     4621 gagaccatgt cgcaaacggc caccgaaccg cgcaccttca gtgagaccga gctggctgcc
     4681 ctcgttgcag atcgtgtaac gcggcagacc gctgaggcca ccaaggagcg cgacgaggca
     4741 gtcgcccgag ccacggcagc agagaacaag ctggacgccg cggtgctggc acagaaggca
     4801 gccgaagaca aggccaaggc cgccgaagag gctctcgaga cccagaagct cgaagccgag
     4861 aaggcagccg agagcgcgac caagaaggac gcccggatca agcagatccg cgaagtcgcc
     4921 aagcacatca ccgacgagac gttcttcacg gacgaggctc gggtgaagcg ggtgatcgcc
     4981 atggacgacg acgcattcac cggctatctc gacgacctga agcgggccgc gggcaacgcc
     5041 accaaggacg gcggctccac gccagctaca ccgccacgtg agacggcgat ggctggcgac
     5101 gtcgcgaacg tcaccacccc gaccggtaag gcaacggctt cggctgcatt cctgggacgc
     5161 cggttcggcg ccgcagaagc tgcttcaacg acgaccacct gagaggaggt caacaccaat
     5221 ggcagacgac tacgggctca acttcgggtt ccgccgaagc ggcgagtcca tgtccgttcg
     5281 tgaaggccgg ctctcggttc cggccggtgg acctgtatgg cccgctgcgg gcagcttcca
     5341 ccagggcgac ttcgtcacca tcgacccggc cagtcccggc ttcttgaagc tggcaccggc
     5401 aaacactccg gcacagacgg gtgttagcgg gctgctggtc caggaggacc cgaacctgtt
     5461 cgacagcatc tacaaccgga tcggcccagg catcctgcac gacagctacg acctcggccg
     5521 catcatcaac aaccgcctgg cgatcatctg ggcgggtgct ggtgtgaagt tctgggtgaa
     5581 gaacaccctg gacgtcacgc ggccggacgg cagcga
//
LOCUS       DATCBJ010000490         6157 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_121971, whole genome shotgun sequence.
ACCESSION   DATCBJ010000490 DATCBJ010000000
VERSION     DATCBJ010000490.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 6157)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6157)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6157
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_121971"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2979)
                     /locus_tag="VII50_09965"
     CDS             complement(<1..2979)
                     /locus_tag="VII50_09965"
                     /inference="COORDINATES: protein motif:HMM:NF016047.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding repeat-containing protein"
                     /protein_id="HEY5051214.1"
                     /translation="MRSEGRLLPGLRRQLTRISTAAAVLLAAPVALMGASAPAAQATA
                     PNDVYYVTAPAMASVGDGIYVSLQVGMSPIGGPPDTFYTGTVHISTTDSAAFTPADTW
                     VPARDQGKVDIGPIVFKTAGSQTITVTDDSYPALNGSAVVDVVQPGPATGFEIATAAN
                     DAFTNDVATNAISDIAIVPVDGDGNVVPTYRGTVHLSSTDPTAQLPVDYTFTASDDQP
                     FVGEARFGSAGTQTITATDTTNSAITGTTDVAVIDPGPAAYMTASMSVQPAVGGTSVG
                     SNGTGRMLVTVWDADGTQDTNYRGTLHFLSIGSGVTVPADYTFTAQDAGAAAFAIGFT
                     GSTQLGVQVTDTVTGMTTGASAWVAPSTGNGAATQLILAPGGRASVGVPTYQYVEADS
                     TYGGLDPTYQDVLNVTTTDPASVHPATYPAEDGSAIVPITFKTAGRQVVTLTDPATGW
                     TVSTAYTVVAPGPTTGFAVSLDDERLLTGDDIDPNLASVVPGVSSDLYIEPVDANGDP
                     TPSYTGAVHVSSSDPAATLPPDATVSDNPGDGGTDIGSVVAATTGTQRVTVTDVSNPS
                     IHGSTPMTVLQAGPAALLAASVADSGENYGTVSLGSAAAVTVVATDAEGFPVPSYQGT
                     VTVSTSDSHAQLPPSVVISGQHGGGTIAQPLVFGTDGVQSLTVTDGTLHGATSANVLT
                     AGPATHLRLDGPSQVLSGSGFSISVEALDAHNDLAPTYTGTVHFTSTAAAATMPANTA
                     FASVARGHLNVAGAKLTSLGFQTITATDTAHASMSGSLVTDVVTAPTGGGGGTGGGGT
                     GGGGTGGGGTGGGGAGGGGAGGGGILAGGPVADRLAGSDRYATAVATSQAEFPDRGAS
                     AVVLARGDDYPDALVGAPLAAAKNAPLLLSSGASLPPVTKAELLRVLATGGTVYVLGG
                     VSAVPASVESELTSLGYHVVRLSGTDRFATAVAVANELGNPSTVLLASGINYPDALAA
                     GPAASHSGGVVLLTNGNAMPT"
     gene            complement(3213..>6157)
                     /locus_tag="VII50_09970"
     CDS             complement(3213..>6157)
                     /locus_tag="VII50_09970"
                     /inference="COORDINATES: protein motif:HMM:NF015649.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="BTAD domain-containing putative transcriptional
                     regulator"
                     /protein_id="HEY5051215.1"
                     /translation="ALGGRRARVALVVLALSQQPVPADRLASMIWADELPATWQVALR
                     GVVRGLRTVLTPIGGGDQRVVRTAPSGYQLAPGIEVDVRLAEQAVRRAAELLADGRAA
                     AALELVESVADQTGDQLLPGEDAEWLAPHRSAADATAARALELAVDAAGRVGDHHTAI
                     AAARRAVTKSPLDERAHRSLIRALDASGDRAGAVQAYEQCRAELAEQLGIDPAAETVE
                     VYLAALRDQSASSAARLPVLTSTFIARDREVAELDAALSQPGLVTVAGRGGVGKSRLA
                     VRVAAARRDFVGGRLWVSLAPVAEDALVASTVALEVGVALGTDDATAVLAEHLAPLGR
                     VLLVLDGCEAVVDGVASLASALLANCPLLTLVVTSRAPLSLEDERVITVEPLDPPTGD
                     DPAALLANAQVRILIDRVHDSGGDLLVDERLAPHVALLLSQCGGLPLALELVAAQLSA
                     MPVGDLLDHLSDVLVDGEDRLRRVVRGSYALLDDDEAAVYRRLAVLDGPVGLPLIRQV
                     VSGGPIAPVRVVRILRELTARGLLAVDRTGARWRYHQDDDLHRFARELLVEHGEEDAA
                     FDRLADAVRNALPDEARVSPAGYRDAITDMLGSIRSLFGAAMQGRADRDRCLELAFRL
                     HRYWAATNVAEGRYWLSRLLASGAESDWTPFATYAMGYLDYWSGDTANAISELETVVR
                     LLEGVESPYTAHSLIYLAGLLDDLDRGAEALEYVRRAIGAAQPFSTDLQVTAAMGMGS
                     VLSERGTPEAARYAADAIALCRDSGSHEQLAAAMPTAAMVCYQVGALDESRAYVAEAR
                     PMHADSRRIARVVLLSTSAGLALADGDLDAAVDFGEAADHEATELGVEREVPLVRSIL
                     ARALLARGDVAAAADRTLAALDAACSITFEFPLAICLETAALVLRAADGLGNDLATLL
                     ATAAVIRERGDRPAPAPLAAAVVALRAELREELLDVATLSAADGGGLAVALLSAVPPR
                     HSDLRAPV"
BASE COUNT         1030 a         2043 c         2152 g          932 t
ORIGIN      
        1 cgtcggcatc gcgttgccat tcgtcagtaa gacgacgcct ccactgtgac tggccgccgg
       61 gccggcagcc agtgcgtcgg ggtagttgat accagacgcc agcaggacgg tgctgggatt
      121 acccagctcg tttgcgaccg caacagcggt cgcgaaccga tcggtgcccg acaaccgcac
      181 gacgtggtac ccaagcgagg tcagctcgga ttcgaccgat gcaggtacag cggacacgcc
      241 gccgagcaca tagacggtac cgcccgttgc caatactcgt agcagctcgg ccttggtcac
      301 aggcggcagg ctggcaccgg acgacaacaa caaaggtgca ttcttggctg ccgcgagcgg
      361 ggcaccgacc agcgcatcgg ggtagtcgtc gccccgagcg aggacgacgg ccgacgcgcc
      421 acggtccggg aactcagctt ggctcgttgc cactgcggtg gcgtagcggt ccgacccggc
      481 gagccgatcc gcgaccgggc cgccggcgag gatcccgccg ccaccagcac cgccgccacc
      541 agcaccgccg ccaccagtac cgccgccacc ggtacccccg ccaccggtac ccccgccacc
      601 ggtaccgccg ccgccacctg ttggagcagt gacgacgtcg gtgacgagtg agccgctcat
      661 gcttgcgtgg gccgtgtcgg tcgcggtgat tgtctggaac ccaaggctgg ttagcttggc
      721 gcccgcaaca ttgaggtgcc cacgggcaac ggaagcgaag gcggtgttcg ctggcatcgt
      781 ggccgctgca gctgtcgagg tgaagtggac agttccggtg taggtgggag cgagatcgtt
      841 gtgggcatcg agggcttcca cggagatgga gaaccccgac cccgagagga cttgcgacgg
      901 accgtcaaga cgaagatgtg ttgccgggcc ggccgtgagc acgttcgcgc tggttgcacc
      961 gtgcaatgtt ccgtcggtaa ccgttaagga ctgcacgcca tctgttccga acacgagcgg
     1021 ctgtgcgatc gtcccgccac cgtgctgtcc ggagatgacg actgatgggg gaagttgagc
     1081 atgggagtcg gaggtgctga cggtgacagt tccttggtag gacggcaccg ggaagccctc
     1141 ggcgtcggtc gccacaacgg tcacagccgc tgcactgcca agcgacacgg tgccgtagtt
     1201 ctcgccgcta tcggcaacgg aggcggctag gagggccgcc ggcccggcct gcaagaccgt
     1261 catcggtgtg ctgccatgaa tcgacggatt tgacacgtct gtcacggtca cccgctgggt
     1321 accggtggtt gctgccacca ccgatccgat gtcggttccg ccatcgccgg ggttgtcact
     1381 gacggtcgcg tccggcggca gcgtggcggc cggatcagag gagctgacat gaaccgctcc
     1441 cgtgtaggac ggcgtcggat cgccgttggc gtcgacgggc tcgatgtaca gatcgctgga
     1501 gacccccggc accacgctgg ccaggttcgg atcgatgtca tcgcccgtga gcagcctctc
     1561 gtcgtccagg ctgacggcga atccggtggt cggaccgggg gccacgaccg tgtacgccgt
     1621 gctcacggtc catccggtcg cggggtcggt cagcgtcacg acctgccgac cggccgtctt
     1681 gaacgtgatc gggacgatcg cagatccgtc ctcggccgga taagtggcgg ggtgcaccga
     1741 cgcaggatct gtggtcgtca cgttgagcac gtcctggtac gtcggatcga gcccgccgta
     1801 cgtcgagtca gcctcgacgt actggtacgt cggtacgccg accgaggcgc ggccgccagg
     1861 ggcaaggatc aactgagtcg cggcgccgtt accagtgctc ggcgcgaccc atgcggaggc
     1921 tccggtagtc atgccggtga cagtgtcagt cacctggacg ccgagttggg tactgcccgt
     1981 gaagccgatc gcaaaggctg ccgcgccggc gtcctgcgcg gtgaaggtgt agtcggccgg
     2041 caccgtcacg ccggagccga tgctcaagaa atgcaacgtg ccgcggtagt tcgtgtcctg
     2101 cgtgccgtcg gcatcccaca ccgtcacgag catgcgcccg gtgccattag agccgacgga
     2161 cgttccccca acagcaggct gaacagacat tgatgccgtc atgtatgccg caggaccagg
     2221 atcgatgaca gccacgtctg ttgtacccgt gatcgcggaa ttggtggtgt cggtcgcagt
     2281 gatggtttgc gttcctgcgc tgccgaaccg agcctcgcca acgaacggct ggtcgtcgct
     2341 ggccgtgaat gtgtagtcga ccggtagttg ggcggtcggg tcagttgagc tcaggtgcac
     2401 ggttccgcga tacgtgggca cgacatttcc gtccccgtct acgggcacga tcgcgatgtc
     2461 gctgatcgcg ttcgtggcga cgtcattggt gaatgcgtcg ttggcggcgg tcgcgatctc
     2521 gaaccctgtt gccggccctg gctgcacgac gtcaacgacg gccgaaccgt tcagggccgg
     2581 gtaggaatcg tcggtcacgg taatcgtctg cgacccggcg gtcttgaaca cgatggggcc
     2641 gatgtcgacc ttgccctggt cgcgtgcggg cacccatgtg tcggccggcg tgaacgcggc
     2701 cgaatcagtg gtcgagatgt gcacagtgcc ggtgtaaaag gtgtccgggg gcccacctat
     2761 ggggctcatc cccacctgta gggagacgta gatgccatcg ccgaccgaag ccatggcagg
     2821 ggcggtgacg tagtacacgt cgttcggggc ggtcgcctga gcagcgggcg cggacgcacc
     2881 cataagggcc accggcgcgg ctaacaacac cgctgccgca gtgctgatcc gcgtcagttg
     2941 gcggcgcaga ccgggaagca gacgcccttc ggagcgcacg aagcttgcct ggttggccga
     3001 cgacacatga tcccccgtgt tgctgatgct ctttcgtaag cgcaagagcg cgcccggttc
     3061 agttggcccg gcgtgcccca cctgcactct cactgtaggt gggtgcgctg ccacccatcg
     3121 gcactttgtc ggaatcgacc cagccgcagc tccccgccgg cctgaatcgc cattacctgg
     3181 gtgttacgca tgcgcgatgg agtgcaagtc cgttacaccg gcgctcggag gtcactgtgt
     3241 cgaggcggga cggcggaaag cagcgcgaca gcgagtccgc cgccgtccgc cgcgctcaaa
     3301 gtcgcgacgt cgagcaactc ctcccgcaac tccgcgcgca gcgcgacgac ggccgcggcg
     3361 agcggcgcgg gagccggccg gtcccctcgc tcccggatga cagcggccgt cgcgaggaga
     3421 gtggcgaggt cgttgcccaa cccgtcggcg gcgcgcaaga cgagagcggc ggtctcgagg
     3481 caaatcgcga gcgggaactc gaaggtgatg gagcaggcgg cgtcgagcgc ggcgagcgtc
     3541 cggtcagccg cggcggcgac gtcgccccgg gcaagcagcg cccgcgcaag aatcgaccgg
     3601 accagcggca cttcccgttc gacgcctagc tcggtggcct catggtcggc ggcctcgccg
     3661 aagtcgaccg ccgcgtcaag gtcaccgtcg gccaaggcaa gtccggcgga cgtcgacagc
     3721 agcacgactc gcgcaatgcg gcggctgtcg gcgtgcatgg gacgagcctc cgcgacataa
     3781 gcgcgcgatt cgtccagcgc gcccacctgg taacacacca tcgccgcggt cggcatggct
     3841 gcagctaact gctcgtgcga accgctatcc cgacacaacg caatcgcgtc ggcggcgtac
     3901 cgcgcggcct cgggagtgcc gcgttcggac agcacgctgc ccatgcccat cgccgccgtc
     3961 acctgaaggt cggtgctgaa cggctgcgcg gcgccaatcg cgcgacgcac gtactcgagg
     4021 gcttcggcgc cgcggtcgag gtcgtcgagc agcccggcca gatagatcag cgaatgcgcg
     4081 gtgtacgggg actcgactcc ctcgagcagc cgcaccactg tttccagttc gctgatcgcg
     4141 ttcgcggtgt cgcccgacca gtagtcgagg tagcccatcg cgtaggtcgc aaacggcgtc
     4201 cagtcgcttt ctgcgccgct ggcgagcaat cgggaaagcc aataacggcc ctccgccacg
     4261 ttcgtcgcgg cccaatagcg atgcaggcgg aaggcgagtt cgaggcatcg atcgcggtcg
     4321 gcgcggccct gcatcgcggc gccgaacagc gagcggatgg aaccgagcat gtcggtgatt
     4381 gcgtcgcgat agccagcggg tgacacccgc gcctcgtcgg gcaatgcgtt gcgcaccgcg
     4441 tcggcgaggc ggtcgaacgc ggcgtcctcc tcgccatgtt cgacgagtag ttcgcgggca
     4501 aagcgatgca gatcgtcgtc ctggtgatag cgccatcgcg caccggtgcg gtcgacggcg
     4561 agcaggccgc gcgcggtgag ctcacgcaag atgcggacga cccgaaccgg cgcgatcggt
     4621 ccgcccgaga ccacctgccg gatcagcggc aagcccaccg gaccgtcgag aacggcgagg
     4681 cgccggtaga cggccgcttc gtcgtcgtcc agcaaggcat aactaccgcg gacgacgcgg
     4741 cgcagccgat cttctccgtc gacgagcacg tcggagagat ggtcgagcaa gtcgcctacc
     4801 ggcatcgcgg aaagttgcgc ggcaaccagt tcaagcgcca acggcaggcc accgcattgg
     4861 ctgagtagca aagccacgtg cggtgcgagc ctctcgtcga ccagcaagtc gcctccgctg
     4921 tcgtgcactc ggtcgatcag gatccgcacc tgcgcgttcg ccagcagtgc tgcggggtcg
     4981 tcgcccgttg gtgggtcgag cggttcgacg gtgatgacgc gttcatcttc gagcgagagc
     5041 ggcgcccggc tggtcacgac gagtgtgagt agcgggcaat tagccagcaa cgcgctagcc
     5101 aatgacgcga cgccatcgac gacggcctcg cagccgtcga gcacaagcag aacccggccg
     5161 agcggcgcca aatgttcagc caagacggcg gttgcgtcat cggtgccgag cgcgacgccg
     5221 acctcgagcg caacggtcga cgccaccagc gcatcctcgg caaccggtgc gagtgacacc
     5281 cacagccgac caccgacgaa gtcacgccgg gcggccgcga cccgcaccgc aagtcgcgac
     5341 ttgccgacgc cacctcggcc ggcgaccgtc acgaggccag gctgcgagag agcggcgtcg
     5401 agttccgcga cctcgcgatc acgggcaatg aatgtggatg tcaggacggg cagccgcgcg
     5461 gccgacgacg cgctctggtc gcgcagcgcg gcgagataaa cctcgacggt ttccgcggca
     5521 gggtcaatgc cgagctgctc agcgagctcg gcgcgacact gctcatacgc ctgcacggca
     5581 cccgcgcgat cgcctgacgc gtcgagcgcg cggatcagcg aacgatgcgc gcgctcgtcg
     5641 agcggtgact tggtgacggc tcggcgggcg gccgcgattg cggtgtggtg gtcgccaacc
     5701 cggccggctg cgtcgacggc cagttcgagc gcgcgggccg ccgtcgcgtc ggcggcgctg
     5761 cgatgtggcg ccagccactc ggcgtcctcg cccggaagca gttgatcgcc ggtctggtcg
     5821 gcgacggact cgaccagctc gagcgcagcg gcggcccgtc cgtcggcgag cagttcggcc
     5881 gcccgccgga cggcctgttc ggcgagccgg acgtcgacct cgatcccagg cgccagctga
     5941 tagccggacg gcgcggtgcg gacgacgcgc tgatcgccgc cgccgatcgg ggtaagaacc
     6001 gtgcgcaggc cgcgcacgac gccgcgcaac gcgacctgcc aggtagcggg gagctcgtcc
     6061 gcccagatca tcgatgcgag ccggtccgct gggacgggct gctgcgagag cgcgaggacg
     6121 acgagtgcga cgcgggcgcg gcggccgccc agtgcct
//
LOCUS       DATCBJ010000491         2879 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_453938, whole genome shotgun sequence.
ACCESSION   DATCBJ010000491 DATCBJ010000000
VERSION     DATCBJ010000491.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2879)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2879)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2879
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_453938"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..689)
                     /locus_tag="VII50_09975"
     CDS             complement(<1..689)
                     /locus_tag="VII50_09975"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HEY5051216.1"
                     /translation="MTTVFLDAGHGGLDPGALGRTTQGRVVQEKAVALTVVLDTEQML
                     HALGFTVVVSRTTDTTVARLTAADVSGTLLTVAGVHADLEARARCADEAHAAVMVSTH
                     FNSASRPGNAGALSVYDSARSFAAGNRRLAELLQATIVAGLDAHGWAIPDAGVVSDTT
                     VGSALSQAAVAYGHLVILGPAMAGYVTSPTTMPGAVVEPLFLTDPFEADIATSPQGQV
                     SIAAAIATAITR"
     gene            complement(1859..>2879)
                     /locus_tag="VII50_09980"
     CDS             complement(1859..>2879)
                     /locus_tag="VII50_09980"
                     /inference="COORDINATES: protein motif:HMM:NF013073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HEY5051217.1"
                     /translation="MDALDNQAEVVTEQFDGAQARLAQAQNAAAAAANAAAAAQAALT
                     AQRVEVSAFAAAAYMSGGVSETVLATTLGSGDPSQTLQKLATLQHLSEQQSQVLLDAN
                     IASLRYDQALATATQDAATAQTLAAGIAAQKKTIDALIAQSQLVLNQLSASERTKLLA
                     AQRAQAAAQQKQAAVALAAWNAARAARPVTRALDRPAVVTRQSVASPVVVSVPQTAAA
                     GGGSSIAERALAAAMSKLGARYVYGAAGARTFDCSGLVQWAYGQAGIRTAHYTGTFWV
                     TYRHIPTNQLRPGDLVFFFRDHHHVGIYIGGGMMINAPQTGDVVRIAPVFRGGGYVGA
                     VRVVG"
BASE COUNT          451 a          969 c          968 g          491 t
ORIGIN      
        1 cgtgtaatgg ctgtcgcgat ggcagcggcg atactgactt gcccttgagg actggtcgct
       61 atgtctgctt cgaacggatc ggtcaagaac aacggctcaa caacggcgcc cggcatcgtc
      121 gtcggcgacg tgacgtagcc agccatcgcc ggccccagga tgaccaagtg gccgtacgcg
      181 acagccgcct gtgacagcgc actccccacc gtcgtatcgg agacaacccc cgcgtctggg
      241 attgcccacc catgcgcatc gaggcccgcg acgattgtcg cttgaagcag ctccgccaac
      301 cggcggttgc ctgccgcaaa ggaccgtgcg ctgtcgtaca cgctcaacgc gccagcgttg
      361 cctggccttg aggcactgtt gaaatgcgtc gacaccatca ccgccgcgtg cgcttcgtcg
      421 gcgcaacgcg cgcgtgcttc gagatcggca tggactcctg cgacggtgag caatgtgccg
      481 ctgacatcgg cggcggtcag ccgtgcgacc gtcgtgtcgg ttgtccgcga caccaccacg
      541 gtgaatccta gggcgtgcag catttgttcc gtgtcgagca caaccgtgag cgctaccgcc
      601 ttctcctgca ccacccgccc ctgggtcgta cgacccaaag cgcccggatc aaggccaccg
      661 tgccccgcat cgagaaacac ggtggtcacc cgcctcgcac caaccgacgg gtaggacatg
      721 caagcccctg gcgcaaagtt cgcggcgtcc accgaaacag gcgctgacgc accagccagc
      781 gtcggcacag gaggaggcgg cgatgatatg accgggccta cagttccggg ggacggcggt
      841 gtcgacgtgc tcgcgagcgg cgcagtaccg accccatgcg aaatgccggc cgcatgcgta
      901 gtacacgccg caacaacgaa tgcgggcacc gccgccaccc agcggtagcg cggcctcctg
      961 cgaaacaggc ggccggcacg gtgaacctgg tcgcgtgtcg ggacgacgac ctgtctcgcg
     1021 gcgccgcgat gccagctggc gcggccgttc ctcgcgtggc tcactggtgt ctccatcatc
     1081 cacagccggg acgcatcgga ccttcgtcgg gttgcaccac tgaagatcca acgtgcttcc
     1141 gcgcgagata gttgcgctgc gtcgaggcgt cagcttcgcg atgtcggccg tggggtggcc
     1201 cgtccacgaa acctcgcggc agcgacggtc agcccgcacc gtcggccagc tcgcctctcc
     1261 cccgacacgg gtaggaaccg cctccgtcgc ggaaacgtct gatgtcgggc cgagatcacg
     1321 cgtacttcgg atcgaagcgc atggtggtca gtcgccgctt gagcctgggc gtcgtagtgt
     1381 tcggcacgac tgtcgtggtt atgtccggtc gcgttccgca tttccccgtc tggcagccgg
     1441 gtcatgcccg tccggatccc tgctcgggcc gggccaaggg accgccatcg atgacgtcga
     1501 ttccgccaag attggcggca cggtgttcgt agccgagcca ggcctgaaac cctcggtgct
     1561 gcagcactca taactagggc cagtagtggc gcacgttcga tgctgcgtgc caggggcgcc
     1621 aaccgatcgg ggtgcaccac cgttggtgga cgggacagcc gcgtgctccc agtcggaacc
     1681 caggttcgtt cggtgacagg attcccgccc gggtgtcgac tcgcggcagt tgtcggcacc
     1741 agggtgcggc tgtacctggc ccaatcgctg gagtcgaacg gcacgagccc gcgtgcgtgc
     1801 cgggtaggcg cgccgaccgc gagcccgcgc tcaggcccgc gcggcggggc gtcgtcttct
     1861 agccgacgac gcgtacggcg ccgacgtagc cgccgccgcg gaacaccggg gcgatccgaa
     1921 cgacgtcacc ggtttgtggt gcgttgatca tcattccgcc gccgatgtag attccgacgt
     1981 ggtggtgatc acggaagaag aacacaaggt caccgggtcg tagctggttc gttggtatgt
     2041 gccggtaggt gacccagaag gtgccggtgt agtgggcggt tctgatacca gcctggccgt
     2101 aggcccattg cacaagacca gagcaatcga aggtccgcgc cccggccgcc ccgtagacgt
     2161 agcgggcacc gagcttgctc atcgcggcgg cgagggcgcg ctccgcgatg ctcgacccgc
     2221 cgcccgcggc cgcggtttgc ggtaccgaca cgaccaccgg cgatgcaacc gattgccgag
     2281 tcaccacggc cggccggtca agtgctcgtg tcaccgggcg cgcggcacgt gccgcgttcc
     2341 acgctgccag tgcaacggcg gcctgcttct gctgggctgc ggcttgggct cgttgtgctg
     2401 cgagcagttt cgtccgctcg ctggcgctga gctggttgag caccagctgg ctctgggcga
     2461 ttagggcgtc gatggtcttc ttctgcgcag cgatgccggc ggcgagggtt tgcgcggtcg
     2521 cggcgtcctg ggtcgcggtt gccaacgctt ggtcgtaacg caggctcgcg atgttggcgt
     2581 cgagcagcac ttgggattgc tgttcgctga ggtgctgcaa ggtcgcgagt ttctgcaagg
     2641 tctgcgatgg gtcgccacta cccaacgtgg tggcaagcac cgtctcgctg acaccgccgg
     2701 acatgtacgc ggcggccgcg aacgcactca cttccactcg ctgcgcggtg agcgcggcct
     2761 gcgcggctgc cgcggcgttc gcggcggccg cagccgcgtt ctgcgcttga gcaagccgag
     2821 cctgcgcgcc gtcgaactgc tcggtgacaa cctccgcctg gttgtccaac gcgtccatc
//
LOCUS       DATCBJ010000492         2361 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_100660, whole genome shotgun sequence.
ACCESSION   DATCBJ010000492 DATCBJ010000000
VERSION     DATCBJ010000492.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2361)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2361)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2361
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_100660"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..299
                     /locus_tag="VII50_09985"
     CDS             <1..299
                     /locus_tag="VII50_09985"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY5051218.1"
                     /translation="SATARRYSGHNQNWLDLLRSGPPMALPAATLATMSSWTVPAMLI
                     HGRDDRVAHFEASLRMLAMVPNSRMVLLNRCGHWAQLEHAAEFNRLVDGFVANN"
     gene            313..648
                     /locus_tag="VII50_09990"
     CDS             313..648
                     /locus_tag="VII50_09990"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: protein motif:HMM:NF007422.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-phenylpropionate/cinnamic acid
                     dioxygenase ferredoxin subunit"
                     /protein_id="HEY5051219.1"
                     /translation="MSITSTRVAWTRVCAVADLPSGEATTLDIDPPIAVFSVDGSFYA
                     IDDTCTHETYSLAEGYIEGARVECALHFAQFDLRTGEALCLPARIGVRTYPVKTENGH
                     IFVDVGRRG"
     gene            721..1509
                     /locus_tag="VII50_09995"
     CDS             721..1509
                     /locus_tag="VII50_09995"
                     /inference="COORDINATES: protein motif:HMM:NF013706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HEY5051220.1"
                     /translation="MTAIDEAADRLRAAAQAGRPCAPVRDVLPAGAIGDAYAVQQVNT
                     QRALDGGGRLVGWKIGLTAPAVQRQLGVGQPDFGVLLADGAFGDDEPIALQRLLQPAV
                     EAEVAFVLDGDLREAPVTAVDVLRATAFVLPVIEVADSRIAGWDITIVDTIADNASSG
                     VFVLGPNPTRLSDVDLRAVRMTMTCEGEKVSEGCGADCLGHPVNAVVWLANTLAALGR
                     PLEAGHVVLSGALGPMVTVGGPGQYEAVLGGLGSVRATFTRDQT"
     gene            1506..>2361
                     /locus_tag="VII50_10000"
     CDS             1506..>2361
                     /locus_tag="VII50_10000"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009078908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008774 - acetaldehyde dehydrogenase
                     (acetylating) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetaldehyde dehydrogenase (acetylating)"
                     /protein_id="HEY5051221.1"
                     /translation="MTGSTRVAVIGPGNIGTDLMIKVLRTSSVLEMGAMVGVDPASDG
                     LARAARMGVPTTADGVDGLLAMPELADIGIIFDATSARAHIANAAKLAPLGKQLVDLT
                     PAAIGPFVVPVVNIEEHLDAPNVNMVTCGGQATIPIVAAVSSVAPVAYGEIVASLASR
                     SAGPGTRANIDEFTETTAHAIEQVGGAARGKAIIVLNPAEPPLLMRDTVFCLVEDGDT
                     KEIADAIDAMVERVQGYVRGYRLKQTVQFERYTDRDPLVAPDLGWNGPGLKVSVFLEV
                     EGAAHYLPA"
BASE COUNT          390 a          796 c          795 g          380 t
ORIGIN      
        1 ggtccgcgac cgctcgccgg tactccgggc acaaccagaa ctggctcgat ctcctccgct
       61 ccggtccgcc gatggcactt cccgcagcga ccctagcgac gatgtcgagc tggaccgtgc
      121 cggccatgct gatccacggg cgggacgacc gggtcgccca tttcgaggca agcctgcgca
      181 tgttggccat ggtgccgaac tcacgcatgg tgcttctcaa ccggtgcggc cactgggcac
      241 aactcgagca cgccgccgaa ttcaaccgtc tcgtcgacgg gttcgttgcc aacaactaga
      301 aggaaggcct caatgtctat tacgagcacg cgcgtcgctt ggactcgagt gtgcgcagtc
      361 gcggatcttc cttcaggaga agccacgacg ctggacatag atccgcccat cgcggtgttc
      421 agtgtcgacg gctcgttcta cgccatcgac gacacgtgca ctcacgagac gtattccttg
      481 gccgagggct acatcgaggg cgcacgggtg gagtgcgcgt tgcatttcgc gcagtttgat
      541 cttagaacgg gagaagcgct ctgcttgccg gcaagaatcg gcgtgcggac ctatcccgtg
      601 aagacggaga acggccacat cttcgtggac gtcggccgcc ggggctgagc ttgcaggacg
      661 caggaggact cattgcggtc ctcccgcgaa cgtcatccgc tgcacgacga aagggacgcc
      721 atgacggcaa tcgacgaggc tgctgaccgg ctccgtgccg ccgcgcaagc cggccggccg
      781 tgcgcacccg tgcgtgacgt gctgccggcc ggcgcgatcg gcgacgcata cgccgtccag
      841 caggtcaata cccaacgggc gctcgacggc ggggggcgcc tagtcggctg gaagatcggt
      901 ctcaccgcgc ccgctgtcca gcggcaactc ggtgtcggcc agcccgactt cggcgtgctg
      961 ctggccgacg gggcgttcgg tgacgatgaa ccgatcgcgc ttcagcgcct cctgcagccc
     1021 gcggtcgagg ccgaggtcgc cttcgtcctc gatggcgacc tgcgcgaagc acccgtcacg
     1081 gccgtcgacg tgctccgggc gaccgcgttc gtgctgccgg tgatcgaagt cgccgattcc
     1141 cggatcgccg gctgggacat cacgatcgtc gacaccattg ccgacaacgc gtcgtcaggg
     1201 gtgttcgtgc tcggcccgaa cccgacgcgg ctgtccgacg tcgacctgcg tgcggtgcgg
     1261 atgacgatga cctgcgaagg cgagaaggtg tcggagggct gcggcgcgga ctgtctgggg
     1321 catccggtga acgcggtcgt gtggctcgcg aacaccctcg cggccctagg ccgtccgctc
     1381 gaggccggcc acgtcgtgct gtccggggcg ctcgggccga tggtgaccgt cggcggaccg
     1441 ggacagtacg aggcggtgct cggcggactc ggctccgtcc gcgccacgtt caccagggac
     1501 cagacatgac cggctcgacc agggtcgcgg tcatcggacc cggcaacatc gggaccgacc
     1561 tgatgatcaa ggtcctccgc acctcgtcgg tcctcgagat gggtgcgatg gtgggtgtcg
     1621 acccggcctc agacgggctc gcccgggcag cccggatggg tgttcccacc accgcggacg
     1681 gggtagacgg cctgctggcc atgccggaac tcgccgacat cggcatcatc tttgacgcca
     1741 cgtcggcgcg tgcccacatc gccaacgccg cgaagctcgc cccgctcggc aagcagctcg
     1801 tcgatctgac cccggctgcc atcggaccgt ttgtcgtgcc ggtggtgaac atcgaggagc
     1861 acctcgacgc gccgaacgtg aacatggtca cctgtggcgg gcaggcgacg atcccgatag
     1921 tcgccgcagt gtcatcggtg gccccggtcg cctacgggga aatcgtcgcg tctctcgcgt
     1981 cacgctcggc aggtccgggc acccgggcca acatcgacga attcaccgag accaccgccc
     2041 acgccatcga gcaggtaggc ggggccgcaa gaggcaaggc gatcatcgtg ctcaacccgg
     2101 ccgagccgcc cttgctcatg cgtgacacgg tgttctgcct cgtcgaagac ggggacacga
     2161 aagagatcgc cgacgcgatc gatgcgatgg tcgagcgggt gcagggctac gtgcgcggct
     2221 accggctcaa gcagaccgtg caattcgagc gctacaccga ccgcgacccg ctggtggctc
     2281 ccgatctcgg atggaacggg ccaggactga aggtgagcgt cttcctcgaa gttgaagggg
     2341 ctgcccacta cctgcccgcc t
//
LOCUS       DATCBJ010000493         7572 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_490978, whole genome shotgun sequence.
ACCESSION   DATCBJ010000493 DATCBJ010000000
VERSION     DATCBJ010000493.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 7572)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7572)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7572
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_490978"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..948
                     /locus_tag="VII50_10005"
     CDS             <1..948
                     /locus_tag="VII50_10005"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY5051222.1"
                     /translation="KSAPTLDLFGSGGLFGETIIIAVPLILGALAGVVCERSGVINVA
                     IEGQMLVGAFAGAMFGTISKNAAVGTIAGMLAGALIAALLAVFAIKYVVNQVVLGVVL
                     NLLALGLTGFLYDAVMAPNSNSYNNVGTLSVINIPVLGNIPIVGPLLFDQTIIVYLTY
                     VLIFAVDIVLFRTRWGLRTRAVGEHPKAADTVGINVRRTRYINVLVGGMLAGLAGASL
                     TIGDSSGFVKNMTGGRGFIALAAVIFGRWSPRGAVGAALLFGFATSLQTVLSFIGTPV
                     QIPSDFLGMLPYLATIFAVAGLVGRVRAPAADGEPYEGR"
     gene            945..1370
                     /locus_tag="VII50_10010"
     CDS             945..1370
                     /locus_tag="VII50_10010"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008600100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009972 - cytidine deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HEY5051223.1"
                     /translation="MTSDRGDVPINWPALRTAAQAVMTLAYAPYSEFAVGAAALVDDG
                     RIVVGCNVENASYGLTLCAECGVVSALSASGGGRLVALACVDGDGAPLMPCGRCRQLL
                     WEHGGPHMLIDHPFGPLPLAELLPYAFGPDDLGTEPRGT"
     gene            1373..2656
                     /locus_tag="VII50_10015"
     CDS             1373..2656
                     /locus_tag="VII50_10015"
                     /EC_number="2.4.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009072567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006206 - pyrimidine nucleobase metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HEY5051224.1"
                     /translation="MDVVDLIRSKRDGSVLSDDAIDWVIAAYTRGEVAEEQMSALLMA
                     IFFVGLTAEELNRWTAAMIASGERLSFPGLGRPTTDKHSTGGVGDKITLPLTPIVAAC
                     GAAVPQLSGRGLGHTGGTLDKLESIRGWRASISNDEMRAQLRDVGAVVCAAGAGLAPA
                     DRKLYALRDVTATVESIPLIASSIMSKKIAEGTSALVLDVKVGVGAFMKDLGRARELA
                     TTMVGLGLAHGVNTVALLTAMDTPLGLTAGNALEVAESLEVLAGGGPADVVELTVALA
                     REMLASCGLTGGKDPADAISDGSAMDSWNAMIRAQGGDPAAPLPVAPHQHDVVASGSG
                     RVVRMDALAVGVAAWRLGAGRARKEDAVSFGAGVQMHAKPGDIVTMGSPLFTLYADDE
                     ARFERALDALTGAVDIASADATWAKPKLVLERIGV"
     gene            2656..3750
                     /locus_tag="VII50_10020"
     CDS             2656..3750
                     /locus_tag="VII50_10020"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009072571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004000 - adenosine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009168 - purine ribonucleoside
                     monophosphate biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HEY5051225.1"
                     /translation="MPATREQIARAPKALLHDHLDGGLRPATVIDLARAVGYSKLPSY
                     DAGELGAWFVRAASSGSLELYLETFAHTFGVMQTVDSLVRVAAECAEDLAADNVVYAE
                     VRFAPELHVDEGLSLDAVVEAVLEGFRLGSEGRGIRVVVLLTALRHAARSFEIAELAI
                     RYRDAGVVGFDIAGAEAGYPPTRHLDAFQYMQRENGHFTIHAGEGFGLPSIWEALQWC
                     GADRLGHGVRIVDDITMVDGQTVLGRLAAYVRDNRVPLEMCPSSNVQTGAARSIAEHP
                     IGLLRKLYFRVTVNTDNRLMSGTSMGIEFEKLVDAFGYDLDDLQWFTINALKSAFIPF
                     DQRLALINDVVKPGYAALRNPAALRTTVED"
     gene            3752..4678
                     /locus_tag="VII50_10025"
     CDS             3752..4678
                     /locus_tag="VII50_10025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020657969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine-5'-phosphate glycosidase"
                     /protein_id="HEY5051226.1"
                     /translation="MSPLVKISEQVRTAVDEGRPVVALESTIIAHGLPRPENLQAAHD
                     FEAILTDAGVVPATIAVIDGVPTVGLDAGELERIANEDIPKVSSRDLAVAVGRKSSGA
                     TTVAATSTLAALAGIRVFATGGLGGVHRAAQESFDESADLVTLSRTRITVICAGVKSI
                     LDIAATLERLETLNVPVVGYGTNQFPAFYLSDSGFPLEWRVDSPEEVAAIMAAGDQLG
                     SRGALIVANPVPLDRQLDPDVHDRLLIQALHAADEAGLRGKAISPFLLDYFQRESGGR
                     SLVVNLDLARNNVALAGGIAIAWSALPAAATA"
     gene            4684..5565
                     /locus_tag="VII50_10030"
     CDS             4684..5565
                     /locus_tag="VII50_10030"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HEY5051227.1"
                     /translation="MLIVGDVIDDIVVTPLGNVTRDSDTTSRIRATPGGSAANQAAWL
                     AASGVSVTFVARVGAAAVERHRSELEAFGIEAVLIADPDAPTGTIVVMLDADGNRTMY
                     TDRGANLRLTTADVPLSLLDDVDALHVNGYSLFAETPRAAVLSLIEEARRREIVVTAD
                     PSSSAYLEELGALTFLSCTRGATVCLPNLDEGRVLSGKREPRDVLAFLGAHYPVVALK
                     LGSGGVLVGEGGQVELIAPALSSVVDPTGAGDAFCAGFLSSWLDGKTAVDAATAGSLL
                     AARAVATYGGRPQADGS"
     gene            5581..6222
                     /locus_tag="VII50_10035"
     CDS             5581..6222
                     /locus_tag="VII50_10035"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEY5051228.1"
                     /translation="MPEDEHGETRLGPSAQAGQWDARYSERDALWSGRPNGRLVAEIG
                     GLTPGRALDVGCGEGADAIWLARGGWIVTAIDISEVALRRARAAADLAGATVEWICGD
                     ALKMAFPVGSFDLLSMQYPALPKAAGEVAVRTLLDAVRPGGLLLAVYHDLDDEHREHM
                     KSRGVDPADYFEAVELGQLLGDFTVELHAIEPRIDPPPDNPHTADVVLRARRR"
     gene            complement(6228..7109)
                     /locus_tag="VII50_10040"
     CDS             complement(6228..7109)
                     /locus_tag="VII50_10040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009072620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEY5051229.1"
                     /translation="MRAKKPDDRIGVRKTYKLYIGGAFPRSESGRSYPITNHHGDFAA
                     HAALASRKDARDAVVAARAAFGGWSRATAYNRGQVLYRVAEVMEGRRDQFVAEVHDAE
                     GGKPAVAAAAVDAAIDRVVWYAGWTDKVAQVVGAANPVAGPFFDFSVPEATGVVALVA
                     PSSSSLLGLVSVLLPVIATGNTTVVVASEPRPLPAITLTEVLATSDLPGGVVNMLTGR
                     VAEVAPWLASHADVNGLDLTGVPIALWRDLEVAAADNLKRVRRPDDAEPDWSRDPGTD
                     RLTSFLETKTVWHPLGV"
     gene            complement(7114..>7572)
                     /locus_tag="VII50_10045"
     CDS             complement(7114..>7572)
                     /locus_tag="VII50_10045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009072622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEY5051230.1"
                     /translation="TDVGAINSSAQLARIRELSDIGEAEGAERWSPACELPERGYWFP
                     PTLFTGVTQAHRIAREEIFGPVLSVLTFRTPDEAIEKANNTPYGLSAGVWTDKGSRIL
                     WMADRLRAGVVWANTFNKFDPASPFGGYKESGFGREGGRHGLEAYLAKGR"
BASE COUNT         1148 a         2456 c         2596 g         1372 t
ORIGIN      
        1 aagagtgcgc cgacgctcga cctcttcggt tctggtggtc tgttcggcga aaccatcatc
       61 atcgcggtgc cgctcatcct gggcgcgctc gccggtgtcg tgtgcgagcg atcaggtgtc
      121 atcaacgtcg cgatcgaagg ccagatgctg gtcggcgcct tcgcgggcgc gatgttcggc
      181 accatctcga agaacgcggc ggtgggaacg atcgccggca tgctcgcggg cgcgctgatc
      241 gctgcgttgc ttgcggtgtt cgccatcaag tacgtcgtga accaggtggt gctcggcgtc
      301 gtcctgaact tgctcgccct tgggctgacc ggcttcctct acgacgctgt gatggcaccg
      361 aactcgaact cctacaacaa cgtaggcacg ctgtcggtga tcaacatccc ggtgctcggc
      421 aacatcccga tcgtcgggcc gttgctgttc gaccagacca tcatcgtgta cctgacgtac
      481 gtcctcatct tcgccgtcga catcgtgctc ttccgtaccc gatggggatt gcggacccgc
      541 gcggttggtg agcaccccaa ggctgcggac acggtcggca tcaacgtgcg acggacccgc
      601 tacatcaacg ttctggtcgg cggcatgctc gccggtctcg ccggcgcttc gttgacgatc
      661 ggcgacagct cgggctttgt gaagaacatg accggcggtc gcggtttcat cgcgctcgcg
      721 gccgtcatct tcggccggtg gagcccccgt ggtgcggtcg gagctgccct gttgttcggg
      781 ttcgccacct cgctacagac ggtgctttcc ttcatcggca cgcctgtcca gatcccgtcg
      841 gatttcctcg gcatgctgcc ctacctcgcc acgatctttg cggtggcggg tctggtcggc
      901 cgcgtccgtg cgcccgccgc cgacggcgaa ccgtacgagg gccgatgacc tcagatcgtg
      961 gggacgtccc gatcaactgg ccggcgttgc gcaccgcggc acaggccgtg atgacgctgg
     1021 catacgcgcc gtactcggaa ttcgcggttg gtgccgccgc gctcgtcgat gacggtcgga
     1081 tcgtcgtcgg ttgcaacgtc gagaacgcgt cgtacggcct cacgctgtgc gccgagtgcg
     1141 gagtcgtttc tgcactatcc gcgtcaggtg gcgggaggct cgttgcgttg gcctgcgtcg
     1201 atggggacgg tgcgccgctg atgccgtgcg gccgatgccg gcagttgttg tgggagcacg
     1261 gtggtcccca catgctgatc gaccacccgt tcggtccgct gccgcttgcc gagctgcttc
     1321 cctacgcatt cgggcctgac gacctcggca ccgagccgag ggggacctga cggtggatgt
     1381 cgtcgacctg atccgttcga agcgcgacgg gagcgttctc agcgacgacg cgatcgactg
     1441 ggtgatcgct gcgtatacca ggggcgaggt cgccgaggaa cagatgtcgg cgctgctgat
     1501 ggcgatcttc ttcgttggcc ttacagcgga agaactcaat cgctggacgg cggcgatgat
     1561 cgcgtcgggt gagcggctga gttttcctgg gctcggcaga ccgacgacag acaagcactc
     1621 gacgggtggt gtgggtgaca agatcaccct cccactcacg ccgatcgtcg cggcgtgcgg
     1681 cgcagcggta ccgcagctca gtggccgcgg gctcggccac accggtggca ccctcgacaa
     1741 gctcgagtcg atacgcggct ggcgggcgtc gatctccaac gacgagatgc gtgctcagtt
     1801 gcgcgatgtt ggcgccgtgg tctgcgcggc cggcgctggg ctcgcgccgg cggaccgcaa
     1861 gctctacgcc ttgcgcgacg tcacggccac cgtcgaatcc attccgttga tcgccagctc
     1921 catcatgagc aagaagatcg ccgagggcac ctctgcgttg gtgctcgatg tgaaggtcgg
     1981 agtcggtgcc ttcatgaagg atctcggccg cgcccgcgaa ctggcgacga cgatggttgg
     2041 cctcggcttg gcacatggcg tcaacacggt cgcactgctg acggcgatgg acactccgct
     2101 tggccttacg gccggcaacg cccttgaggt ggcggagtcg ctcgaggtac ttgccggcgg
     2161 cggaccggcc gatgtcgtcg aacttaccgt cgcgcttgcg cgcgagatgc tcgcctcctg
     2221 cggtttgacc gggggcaagg acccggcgga cgctatttcc gatgggtcgg cgatggactc
     2281 gtggaacgcg atgatccggg cacagggcgg cgatccggct gcgccactgc cggttgcgcc
     2341 ccaccagcac gacgtcgtgg catcggggtc gggccgggtc gtccgcatgg acgcgcttgc
     2401 cgtcggcgtc gccgcgtggc ggttgggcgc cggccgcgcg cgcaaggagg acgcagtgtc
     2461 gttcggcgca ggcgtgcaga tgcacgcgaa gcccggcgac atcgtcacga tgggcagccc
     2521 gctgttcacc ttgtacgccg acgacgaggc gcggttcgaa cgtgcacttg acgcactcac
     2581 gggtgccgtc gacatcgcct ccgctgacgc cacgtgggcg aagccaaagc tcgttctcga
     2641 gaggattggt gtgtgatgcc ggcgacccgc gagcaaatcg cgcgcgcacc caaggcgttg
     2701 ctgcatgacc acctcgacgg cggcttgcgc ccggccaccg tgatcgacct cgcgcgtgcc
     2761 gtcggctact cgaagctgcc tagctacgat gccggtgagc tgggcgcatg gttcgtgcgc
     2821 gcggcgtcgt caggctcgct cgagctctat ctcgagacgt ttgcccacac cttcggcgtc
     2881 atgcagactg tcgactcact ggtccgggtc gcagccgagt gcgccgaaga tctcgccgca
     2941 gacaacgtgg tctacgccga ggtgcggttt gcaccagaac tgcacgtcga cgaggggctc
     3001 agcctggatg cggtcgtcga ggcggtgctc gagggattcc ggctcggcag cgaaggtcgc
     3061 ggcatcaggg tggtagtgct gctgactgcc ttgcggcacg cagcgcgttc gttcgagatc
     3121 gccgagcttg ccatccgtta ccgcgatgcg ggcgtcgtcg gattcgacat cgcaggcgcc
     3181 gaggcgggct acccgccgac tcgtcacctc gatgcgttcc agtacatgca gcgcgagaac
     3241 ggtcacttta cgatccatgc cggcgagggt ttcggcttgc cgtcgatctg ggaagcactg
     3301 cagtggtgcg gcgcggatcg actcggtcat ggcgtgcgca tcgtcgatga catcacgatg
     3361 gtcgatggcc aaacggttct tggacgactg gccgcttacg tgcgcgacaa ccgagtgccg
     3421 ctggagatgt gcccgtcatc gaacgtgcag actggcgcgg cgcgttcgat cgccgagcac
     3481 ccgatcggcc tgctgcgcaa gctctatttc cgggtcaccg tcaataccga caaccggctg
     3541 atgagcggga cgtcgatggg tatcgagttt gagaagctgg tcgacgcgtt cgggtacgac
     3601 ctcgacgacc tacagtggtt caccatcaac gcattgaaga gtgcattcat cccgttcgac
     3661 caacggcttg cgctcatcaa cgacgtcgtc aagcccgggt acgccgcgct ccggaaccct
     3721 gccgcgctac gaactaccgt ggaggattga cgtgtcacca ctggtgaaga tctccgagca
     3781 ggtccgcacg gccgtcgacg aaggtcgacc ggtggtcgct ctcgagtcga cgatcatcgc
     3841 gcacgggttg ccacgtccgg agaacctgca agccgcgcac gacttcgagg caatcctgac
     3901 cgacgccggt gtagtcccag cgaccattgc ggtcatcgac ggcgtcccga ctgttggact
     3961 cgacgccggc gaactcgaac gcatcgcgaa cgaggacatc ccgaaggtca gcagccgcga
     4021 cctcgcagtc gccgtcggac gcaagtcgag cggcgcaacc actgtcgctg cgacgtcgac
     4081 attggccgca ctcgcgggca ttcgcgtctt tgcaacgggt gggctcggcg gcgtgcaccg
     4141 cgctgcacag gagtcgttcg atgagtcggc cgacttggtc accctgtcgc gcacccgcat
     4201 caccgtcatc tgcgcgggtg tgaaatcaat cctcgacatc gcggcgactc tcgaacgcct
     4261 tgagacgctg aacgtgcccg ttgtcggtta cggcacgaac cagtttccgg ccttctatct
     4321 cagcgattct ggcttcccgc ttgagtggcg ggtcgattcc ccggaggagg tcgcggccat
     4381 catggcggcc ggtgaccagc tcggttctcg cggtgcgctc atcgtcgcga acccggtgcc
     4441 gctcgatcga cagctggatc ccgacgtcca tgaccggttg ctcattcagg cgctgcacgc
     4501 agccgacgaa gctggcttgc gcggcaaggc aatcagcccg ttcctgctcg actacttcca
     4561 gcgcgaaagc ggcggccggt ccctcgtcgt caatctcgat ctcgcgcgga acaacgtcgc
     4621 gctggccggc ggcatcgcga tcgcgtggtc ggcgttgccc gcggcggcca ccgcttgacg
     4681 aggatgctga tcgtcggcga tgtcattgac gacatcgtcg tcacaccgct tggcaacgtc
     4741 acccgcgaca gcgacacgac ctcgcggatt cgcgcgacgc cgggcggttc ggccgcgaac
     4801 caggctgcgt ggctcgccgc gtctggcgtg tccgtgacgt tcgttgcccg agtcggtgcg
     4861 gcggcagtcg agcgacatcg cagcgagctc gaggcgttcg gcatcgaggc cgtgttgatt
     4921 gcggaccctg acgcgccgac cggcacgatc gtggtgatgc tggacgccga cgggaaccga
     4981 accatgtaca ccgaccgtgg cgcgaacctg cggctcacca cggctgacgt gccgctgtcg
     5041 ttgctcgatg acgtcgacgc gctgcacgtc aacgggtatt cactgttcgc cgagacgcct
     5101 cgcgcggctg ttctttcttt gatcgaggaa gcacgtcgcc gcgagatcgt cgtgacggcc
     5161 gacccatcgt cgtccgccta tcttgaagag cttggtgcgt tgacgtttct gtcttgcacg
     5221 cgcggcgcga ccgtgtgcct gccgaacctc gacgaaggcc gggtgctcag cgggaagcga
     5281 gagccgcgcg atgtgctcgc gtttctcggc gcgcactatc cggtggtggc gttgaagctc
     5341 ggatcgggtg gtgttcttgt tggcgagggc gggcaggtcg agctcatcgc gcctgcgttg
     5401 tcatcggtgg tcgatccaac aggtgcgggc gatgccttct gcgcggggtt tcttagctcg
     5461 tggctcgacg ggaagacggc ggtcgatgcg gccacggctg gctcgctgct tgcggcacgt
     5521 gcagtggcga cgtacggcgg ccgaccacag gctgacggca gctgatctag gctgcagagc
     5581 atgcctgagg acgagcacgg tgagacgcgg ctcggcccgt cggcgcaggc cggccagtgg
     5641 gacgcgaggt acagcgagcg cgatgcgttg tggagcgggc ggccgaatgg tcggctcgtc
     5701 gccgagatcg gcggcctcac gccgggccgc gcgctcgacg tcggttgtgg cgagggcgcc
     5761 gacgcgatct ggctcgctcg cggcggctgg atcgtgaccg cgatcgatat ctccgaggtg
     5821 gcgctgcgcc gggcacgggc ggcagcggat ctggccggcg ctacggtcga gtggatctgc
     5881 ggcgacgcgc taaagatggc gttcccggtc ggctcgttcg accttctgtc gatgcagtat
     5941 ccggcgctgc caaaggcggc tggcgaggtc gccgtgcgga cgttgctcga cgcggtacgg
     6001 cccggcgggt tgctgctcgc ggtctaccac gaccttgacg acgagcaccg cgagcatatg
     6061 aagtcccgcg gcgtcgaccc tgcggactac ttcgaggccg tcgaactcgg tcagctgctc
     6121 ggtgacttca cggtcgagct acatgcgatc gagccgcgca tcgacccgcc gccggacaac
     6181 ccgcatactg ccgacgtcgt tctgcgtgcc cggcgccgct gatcgctcta gacgccgagc
     6241 gggtgccaga cggtcttggt ctcgaggaaa ctggtcagcc gatcggtgcc ggggtcgcgt
     6301 gaccagtccg gctccgcgtc gtccggcctc ctcactcgct tgagattgtc ggctgctgcc
     6361 acctcgaggt cgcgccaaag tgcgatcggg accccggtga gatcgaggcc gttgacgtcg
     6421 gcgtgcgaag cgagccaggg tgccacctcg gccacccggc cggtcaacat gttgacgacg
     6481 ccgccgggca ggtcggacgt cgcgagcacc tcggtcaacg tgatcgccgg tagcggtcgt
     6541 ggctcactcg ccaccactac ggtggtgttc ccagtggcga tgaccggcag caacaccgac
     6601 accaggccga gcaggctgga cgacgacggc gcgacgagcg cgacgacgcc ggtggcctcc
     6661 ggtaccgaga aatcgaagaa cggtccggct accggattcg ctgcaccgac gacttgcgcg
     6721 accttgtcgg tccagccggc gtaccagacc acgcggtcga tcgcggcgtc gaccgcagct
     6781 gcggcgacgg cgggcttgcc gccttccgca tcgtggactt cggcgacgaa ctggtcgcgg
     6841 cgcccttcca tgacttcggc tactcgatac agcacctgcc cgcggttgta ggcggtcgcg
     6901 cgcgaccacc ctccgaaggc ggcccgtgcc gcgacgacgg catcacgcgc gtccttgcgc
     6961 gacgcgagtg cggcgtgcgc ggcgaagtcg ccgtggtgat tggtgatggg atagctgcgc
     7021 ccagactcgc tgcgcgggaa ggcgccgcca atgtagagct tgtacgtctt gcggacgccg
     7081 atgcggtcgt cgggcttctt tgcgcgcatc gcgttacctc cccttggcga gataggcttc
     7141 gagaccgtgc cggccgccct cgcgaccgaa gcccgactcc ttgtaaccgc cgaacggcga
     7201 ggccgggtcg aacttgttga atgtgttcgc ccacacgacg cccgcacgca ggcgatcggc
     7261 catccagagg atgcggctgc ccttgtcggt ccagactccg gccgacaacc cgtacggcgt
     7321 gttgttcgcc ttctcgatcg cctcgtccgg cgtgcggaac gtcaggacgg agagcaccgg
     7381 tccgaagatc tcctcgcggg cgatgcgatg cgcttgcgtg acgccggtga acaaggtcgg
     7441 tgggaaccag tagccgcgtt cgggcagctc gcaggccggc gaccaccgct cggcaccttc
     7501 tgcctcgccg atgtcggaga gctcgcgaat gcgagcaagc tgagcggagg agttgatcgc
     7561 gccgacatcg gt
//
LOCUS       DATCBJ010000494         5116 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_306436, whole genome shotgun sequence.
ACCESSION   DATCBJ010000494 DATCBJ010000000
VERSION     DATCBJ010000494.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 5116)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5116)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5116
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_306436"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..285)
                     /locus_tag="VII50_10050"
     CDS             complement(<1..285)
                     /locus_tag="VII50_10050"
                     /inference="COORDINATES: protein motif:HMM:NF013231.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEY5051231.1"
                     /translation="MAAARVLVGVVAQSVADVEDQVTLPQLRVLVMVATRGPLNLAAV
                     ADGLGVHPSNATRTVDRLVAARLVDRREATVDRRNASITLTRSGQQLVDSV"
     gene            510..788
                     /locus_tag="VII50_10055"
     CDS             510..788
                     /locus_tag="VII50_10055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5051232.1"
                     /translation="MSLEMSNSGGRHSRRPADEMGGVQPTTPGQDVMGGKQPTPPGQD
                     VMGGKQPTPSGQDEMGGVQPTPPGQDVMGAKQPTPSGQDEMGGVQPTR"
     gene            1161..2342
                     /locus_tag="VII50_10060"
     CDS             1161..2342
                     /locus_tag="VII50_10060"
                     /inference="COORDINATES: protein motif:HMM:NF005495.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY5051233.1"
                     /translation="MPSDAPGNGYSPVAATRAVTRLAAATSGSEPSAGQEVCEGTAMS
                     VVVPGKSSWRLWMQRSGRQVVVVTGASGGVGRAVARAFGARGAAVGLIARGVKGLAGA
                     ARDVEGAGGEALAMPTDVADFHALQAAGEQVEHEMGPIDVWVNVAFTSVFAPFTKITP
                     DEYKRVTEVSYLGFVYGTKVALEYMVPRDRGTIVQVGSALAYRGIPLQTAYCGAKHAI
                     QGFNEALRCELLHDKSNVHVTMVQLPAVNTPQFSWVLNKLPHRAQPVPPIYEPSVIAD
                     AICYAADHPRRREYWVGGSTVGTLLANAVAPGVLDRYLGKTGYKSQQTPQPEPTDAPV
                     NLWEPADGLDGHDFGARGVFTDRSHSRSPQVWLSQHHRLLGVAGVLLGAGAATVAAAR
                     R"
     gene            2352..4160
                     /locus_tag="VII50_10065"
     CDS             2352..4160
                     /locus_tag="VII50_10065"
                     /inference="COORDINATES: protein motif:HMM:NF012926.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
                     /protein_id="HEY5051234.1"
                     /translation="MALRIEDYALIGDTRTCALVGVDGSIDWLCVPRFDSGAVFAALL
                     GTDENGRWQVAPDGAHRTTQRRYRPDTLVLETEFATGSGLVRVVDCMVMNPGDEGPQL
                     VRLVEGVEGTVAMRSDVVFRPDYGQVLPWFRRSGRRLTIYAGPDAVALDGDVAHTVVD
                     DTASASFTIGAGQRLGIRLAWMGTSTRLPQLTDVAALVERTAAWWCRWAAKCSYAGID
                     RDAVMRSLITLKALSYTPSGGIVAAPTTSLPERLGGVRNWDYRYCWLRDATYTLLALM
                     DGGYTTEAAAWRDWLLRAVAGHPKQMQIMYGLDGERRLTETEIGWLPGYAGSAPVRVG
                     NAAHSQRQLDVYGEVMDALHHARANGLPPDDDAWTLQVRLMNYLQTIWAKPDNGIWEV
                     RGKRQHFVHSKVMAWVAFDRAIKGVEAFGFDGPVDQWRQVRRTIFDEVCERGYDPKRK
                     TFTQYYGSDELDAATLLIASVGFLPADDERIVGTVAAIEQNLLSDGFVQRYTTASSSV
                     DGLPPGEGAFLMCTFWLADNYTLAGRHDEARALFDRLLALRTATGLLSEEYDTTNHRL
                     VGNFPQAFSHVALITTALSLAATAGPAQRRGAGNPT"
     gene            4157..4567
                     /locus_tag="VII50_10070"
     CDS             4157..4567
                     /locus_tag="VII50_10070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5051235.1"
                     /translation="MNSPGRTELLRAAWGTVLLSQPGRVLALCDTAGVPGPAVFAARL
                     LGARQLTQAIITALAPTPRVIRLGAAVDTLHACTGIGLAVLVPRWRRPALADAAVAAG
                     FAVGGQLRVRPPRHRHAPGWLVPVAATRKNTASS"
     gene            complement(4702..>5116)
                     /locus_tag="VII50_10075"
     CDS             complement(4702..>5116)
                     /locus_tag="VII50_10075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEY5051236.1"
                     /translation="TRLSSDDFARLLAFRTSLRRFEHWSASQARAAGLTPMQHQLLLA
                     IKGHTDPRGPTIGEIAEYLLLRPHSAVELVDRGEAAGLVVRKRSDNDARVVRVQLTAN
                     ASRRLSALTSLHVAELRRFVPLLRDLTNAVEETPD"
BASE COUNT          849 a         1590 c         1790 g          887 t
ORIGIN      
        1 gacactgtcg acgagttgct gacccgaccg cgtcagggtg atggacgcgt tccgacggtc
       61 gacggtggcc tctcgacggt caacaagtcg ggccgcaacg agccggtcca cggtgcgcgt
      121 ggcattcgat ggatgcacgc ccagaccgtc cgcaaccgcg gccaagttca acgggccgcg
      181 ggttgcaacc atgaccagga cccttagttg aggcagcgtc acctggtcct cgacatcggc
      241 gaccgattgc gcgacgacac cgacaagcac gcgagctgca gccatcaccg catccacttg
      301 gcggatcgaa tccgcggagg cgactgatct ttggttgcgc gatgccgaca ctcgatcatt
      361 gtgaagcata tgtgctcatg cgcaatcatt gcggcccatc agtttgcgag ccggcgaggt
      421 ttgcggatct ttgacgaggg catcacttgg tttgtaatgg ttgcgttcgc gcaaccaacg
      481 gtaaggcagg cacttagagc ggagagatca tgagtctcga gatgagcaat tccggaggca
      541 gacacagccg ccgtccggcc gacgaaatgg gcggggtcca gccgacgacg cccggtcaag
      601 acgtgatggg cggcaagcag ccgacgccgc ccggtcaaga cgtgatggga ggcaagcagc
      661 cgacgccgtc tggtcaagac gagatgggcg gggtccagcc gacgccgcct ggtcaagacg
      721 tgatgggtgc caagcagccg acgccgtccg gtcaagacga gatgggtggc gtccagccga
      781 cgcgatagct cactagtcga tcagcctgtc agctccgacg accggccggg cctgtgcggc
      841 ctcgcatgcg aatacggcta cgacctcgac tactttcggg ccatgctcgc cgccggtggc
      901 cgtcgatgtc ctgcaagccg atgtcgccgg tggcggtacc accgatggct tcgggtcgcc
      961 gcgctcgcga tcgccgaaca ggtgctgtac acgaatattg tggcccggcg atccatctgg
     1021 acgacgcgac ggtgccgccg aaccttcgtc acctcgagta tttccacgat cacgtgcgcc
     1081 accctaaaga ccgcggacgc cgcgccgtac cgcgtcgcgt ggacccaacg cagtagaccc
     1141 cgtcgaacga catcgcctcg atgccgtcgg atgcgcccgg caacgggtac agcccggtcg
     1201 ccgcgaccag ggcagtgacc cgactcgctg cggccacgtc gggtagtgag ccgtcagcgg
     1261 ggcaggaggt atgcgagggc acggctatgt cggtcgttgt gccaggtaaa tcgtcgtgga
     1321 ggttatggat gcagcggtcg ggccggcagg tggttgtggt cacgggtgcg agcggtggtg
     1381 tgggtcgggc ggtggcgcgc gcgttcgggg ctcggggtgc ggcagtgggg ttgatcgcgc
     1441 gtggcgtgaa gggcttggcg ggtgcggcgc gcgatgtgga aggcgccggc ggggaagcgt
     1501 tggcgatgcc gaccgatgtc gcggatttcc atgcgctgca ggctgcgggt gagcaggtcg
     1561 agcacgagat gggtccgatc gacgtgtggg taaatgtggc tttcacgtcg gtgttcgccc
     1621 cgttcaccaa gatcacaccg gacgagtaca agcgggtgac ggaggtgtcc tatctgggtt
     1681 tcgtctacgg cacgaaggtg gcgttggagt acatggtgcc gcgtgaccgc gggacgatcg
     1741 tgcaggtcgg gtccgcgttg gcgtaccggg ggatcccttt acagacggcg tattgcggtg
     1801 cgaagcacgc gattcagggt ttcaacgagg cgttgcgatg cgagttgttg catgacaaga
     1861 gcaacgtgca tgtgacgatg gtgcagctgc ctgcggtgaa cacgccgcag ttttcgtggg
     1921 tgttgaataa attgccgcac cgcgcgcagc cggtgcctcc gatctatgag ccgtcggtga
     1981 tcgccgatgc gatctgttac gccgcggacc atccacggcg acgcgaatat tgggtcggcg
     2041 ggtctaccgt cggcacgctg ttggcgaacg cggtggcgcc gggggtgttg gaccgttacc
     2101 tcggtaagac cggctataag agtcagcaga caccccaacc ggagccgacc gacgcgccgg
     2161 tgaacctgtg ggagccggcc gacgggttgg acggtcatga ttttggtgcc cgcggagtgt
     2221 tcactgaccg gtcgcattcg cgcagcccac aggtgtggct gtctcagcac cacaggctgc
     2281 tcggcgttgc cggggtgctg ctcggcgcgg gagcggctac ggtggccgcg gcgcggcggt
     2341 gaggtaaagc gatggcattg cgaatcgagg actacgcgct gatcggggac acccgcacgt
     2401 gtgcactggt cggtgtcgac gggtcgatcg actggttgtg cgtcccccga ttcgacagcg
     2461 gcgcggtgtt cgccgcgctg ctcggaaccg atgaaaacgg ccgctggcag gttgcgcccg
     2521 acggcgcgca ccggaccacc cagcgccgct accggcctga cacgttggtg ttggaaacgg
     2581 agtttgctac cggctccggt ctggtgcggg tcgtggactg catggtgatg aaccccggtg
     2641 atgagggtcc gcagctcgtt cggctcgtcg aaggcgtgga aggcaccgtc gcgatgcgca
     2701 gcgatgtggt gtttcgtccc gactacggac aggtgctccc gtggtttcga cgaagcggac
     2761 ggcggttgac gatctacgcg gggccggacg cggtcgcgtt ggacggcgac gtcgcgcata
     2821 cagtcgtgga cgacaccgcg agcgcgtcgt tcacgatcgg agctggccag cgactcggga
     2881 tccggttggc ctggatgggc acgtccaccc ggctgccgca gctaactgac gtggccgcgt
     2941 tggtcgaacg cactgccgca tggtggtgcc ggtgggccgc gaagtgctcc tacgccggga
     3001 ttgatcggga cgcggtgatg cgttcgctga tcacgttgaa agcgttgtcg tacacgccga
     3061 gtggcggcat cgtggccgcg ccgaccactt cactgccgga acgactcggt ggggtccgga
     3121 actgggacta ccgctactgc tggctgcgtg atgccaccta cacgttgctc gcgctgatgg
     3181 acggcggcta caccaccgaa gccgcagcct ggcgtgattg gctgctgcgt gcggtcgccg
     3241 gtcaccccaa gcagatgcaa atcatgtacg ggctggacgg agaacggcgg ctgacggaaa
     3301 ccgaaatcgg ctggctgccc ggctacgccg gttccgcgcc ggtgcgggtc ggcaacgccg
     3361 cgcacagcca acgccaactc gatgtgtacg gcgaggtgat ggacgcgctg catcacgccc
     3421 gcgccaatgg cctaccgcca gacgacgacg cgtggacgct gcaggtgcgg ctgatgaact
     3481 acctgcagac gatctgggcg aaacccgaca acgggatctg ggaggtccgc ggcaaacgcc
     3541 agcacttcgt ccattccaaa gtgatggctt gggtcgcatt cgaccgcgcg atcaaaggcg
     3601 tggaagcctt cgggttcgac ggacctgtcg accagtggcg gcaggtgcgg cgcaccatct
     3661 tcgatgaggt ctgtgaacgt ggctacgacc ccaaacgcaa gacgttcacc cagtactacg
     3721 gctccgatga actcgacgcc gcgacgctgc tgatcgcctc agtcggtttt ttgcctgcgg
     3781 acgacgaacg catcgtcggc acggtggccg cgatcgagca gaacctgctt agcgacgggt
     3841 tcgtgcagcg gtacacgacg gcaagctcgt ctgtcgatgg tctgccgccg ggcgaaggcg
     3901 ccttcttgat gtgcacgttt tggctcgccg acaactacac cctcgccggg cgccacgatg
     3961 aggcgcgcgc tcttttcgat cggctcctag ccctccgcac cgcaaccggc ctgctctctg
     4021 aggaatacga caccaccaac caccgcctcg tcggaaactt tccccaagcg ttcagccatg
     4081 tcgcgcttat caccaccgca ctgagtctgg ccgcgaccgc cggacctgcc caacggcgcg
     4141 gggccggtaa cccgacgtga actcgcccgg gaggaccgag ttgctgcggg ccgcctgggg
     4201 cacagtcttg ctgagccaac caggacgcgt ccttgcgctg tgcgacacag cgggggtacc
     4261 tgggcctgca gtgttcgccg ctcgcctgct cggcgcccgt cagctcacgc aggcgatcat
     4321 cacggcactc gcaccgacac cgcgcgtcat cagactcggc gctgcagtcg acacccttca
     4381 tgcgtgcact ggtatcggct tagcggtgct ggtgccgcgc tggcgacgcc ctgcgcttgc
     4441 cgacgcggcg gttgcagcgg gcttcgcggt cgggggacag ttgcgggtcc ggccgccgcg
     4501 gcaccgtcac gccccggggt ggttggtccc tgtcgccgcg acgaggaaga acacggcttc
     4561 ctcatagccg tcggctcggc agatatctgc cgccccgaca cgtccaggtc gttcgccgcg
     4621 gccgggctgg gcaccgacac ggcacgctta gtaccaccca cgggcagcat gcacgatcgg
     4681 ctcggacggt tgtcgccgat atcagtcagg cgtttcttcc acggcgttcg tcagatcacg
     4741 caggagcgga acgaatcggc gtagctcggc cacgtgcagg gacgtcagcg cggacaggcg
     4801 acggctggcg ttcgcggtca gttggacgcg gaccaccctc gcgtcgttgt cggatcgctt
     4861 gcggaccacg agcccggcgg cttcgccgcg gtcaaccagc tcgacggcgc tgtgcgggcg
     4921 cagcagcagg tactcggcga tctccccgat cgttggcccc cgcggatctg tgtggccttt
     4981 gatcgccagc agcagctgat gttgcatggg cgtgagtcct gccgcgcgcg cttggctggc
     5041 gctccagtgt tcgaaccggc gaaggctggt gcgaaacgcc aacaaccgcg cgaagtcgtc
     5101 gctgctcagc cgggtc
//
LOCUS       DATCBJ010000495         2283 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_48989, whole genome shotgun sequence.
ACCESSION   DATCBJ010000495 DATCBJ010000000
VERSION     DATCBJ010000495.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2283)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2283)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2283
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_48989"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..516
                     /locus_tag="VII50_10080"
     CDS             <1..516
                     /locus_tag="VII50_10080"
                     /inference="COORDINATES: protein motif:HMM:NF013224.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HEY5051237.1"
                     /translation="WRGCAALRSAHGASRQQVALMALDDIDQQILAVLRDHARASFAE
                     VGVQVGLSAPAVKRRVDRLRANGVITGFAAIVDPSALGWTTEAYVELYCQGRTSPTAI
                     AEAVSRHPEVVDACTVSGEADALIHVVAADMRHFEDVLERIGAESFVVRTKSVLVLSR
                     LLRREAATDPN"
     gene            634..1500
                     /locus_tag="VII50_10085"
     CDS             634..1500
                     /locus_tag="VII50_10085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidinotransferase"
                     /protein_id="HEY5051238.1"
                     /translation="MTATVSDSARSTAPGTLMATHERVATHRRYLMCEPRHFDVQYAI
                     NPWMDPSVPVDTELAVQQWRGLVEAYRRLGHDVAQIEPVEGLPDMVFAANGATVANNR
                     VYGAKFLHAERAAEGPAYLSWFRAAGFDAHDARFINEGEGDLLVAGGRLLAGTGFRTV
                     PEAHAEAGEALGLPVVTLELVDPHYYHLDTALCVLGPDEIAYFPGAFSPASQARLREL
                     YPNAILATASDAAVLGLNAVSDGYHVVIPAEAVGLAATLLARGYHPVPVQLSELRKAG
                     GGPKCCTLELRP"
     gene            1497..>2283
                     /locus_tag="VII50_10090"
     CDS             1497..>2283
                     /locus_tag="VII50_10090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEY5051239.1"
                     /translation="MTPASDVLVSDITVNGGLTSTAPQYIERDDAHAAHTYHPLPVVL
                     REGSGVWVTDVDGRRYLDCLAGYSALNFGHGHPVIVAAARDQLERLTLTSRAFHHDLL
                     GSFTAELAQLCGMEAVLPMNSGAEGVESALKLARKWGYEVKGVQPDQANIVVARNNFH
                     GRTISVVSFSSDPDAKNNFGPFTPGFREVAFGDADALASAIDVNTVAVLIEPIQGEAG
                     VVIPPVGYLRAVRDICTARGVLFMADEIQSGLGRTGRTFACEHE"
BASE COUNT          372 a          738 c          763 g          410 t
ORIGIN      
        1 tggcgtggct gcgccgcgct acggtccgcg catggcgctt cgcgacaaca ggtcgctctg
       61 atggccctcg acgacatcga tcagcagatc ctcgcggtgt tgcgcgacca cgcgcgggcc
      121 agcttcgccg aggtcggggt gcaggttggt ctgagtgcac cggcagtgaa gcgccgcgtc
      181 gacagattgc gggccaacgg ggtcatcacc ggattcgcgg cgatcgtcga tccgtcagca
      241 ctcggctgga cgacggaggc ttacgtcgag ttgtactgcc agggccggac gtcgccaacc
      301 gcaattgccg aggcagtgtc gcgccacccg gaggtggtgg acgcctgcac cgtctccggc
      361 gaggccgacg ccctcatcca cgtggtggcc gccgacatga ggcacttcga ggacgtgctg
      421 gagcggatcg gcgcggagag tttcgtggtc cgcacgaaga gcgtgctggt gctgtcgcgg
      481 ttgctccgtc gtgaggcggc gaccgatccg aactagaact tccctccggc gcgcaacaaa
      541 tcgccgtctg ggagcgttct cgtggtggaa tctgccgctt agacgcaagg gaacaggctt
      601 gttcgaaagc ctggggtcac ccatggttag aggatgaccg caaccgtgag tgactccgcc
      661 agatcgacag cgccgggcac cctgatggcc acacatgagc gggtggcgac acatcgccgc
      721 tatctcatgt gcgagccgcg gcacttcgat gtgcagtacg cgatcaaccc gtggatggat
      781 ccctcggtgc cggtcgacac cgaactcgcc gtccagcagt ggcgcggtct cgtcgaggcc
      841 tatcgccgtc ttggtcacga tgtcgcgcag atcgagcctg tcgagggatt acccgacatg
      901 gtgtttgcgg ccaatggcgc gacggtcgcc aacaatcgtg tgtacggcgc caagttcctg
      961 cacgcggagc gcgctgccga gggtccggcc tacctcagct ggtttcgcgc ggccggcttc
     1021 gacgcccacg atgcgaggtt catcaacgag ggcgagggcg acttgctcgt agcgggtggc
     1081 cggttgctcg ccggcaccgg ctttcggacc gttccggagg cgcacgccga agcaggcgag
     1141 gcccttggtc ttccggtggt cactctcgag ctcgtcgatc cgcactacta ccacctcgac
     1201 accgcgttgt gcgtgctcgg acccgacgaa attgcttact tcccaggcgc tttcagcccg
     1261 gcaagccagg caaggctgcg cgagctctat cccaacgcga tccttgctac tgcatcggac
     1321 gccgcggtgc tcgggctcaa cgcggtcagc gacggttacc acgtcgtgat ccctgcggag
     1381 gccgtcggtc tggcggccac gttgctcgcg cgcggttacc atccggtgcc tgtgcagctc
     1441 agtgaattgc gcaaagccgg cggtgggccg aagtgctgca cgctggagct gcggccatga
     1501 cgccggctag cgatgtgctg gtcagtgaca tcaccgtgaa cggcggcctg acgtcgaccg
     1561 ccccgcagta catcgaaaga gacgacgcgc atgccgcgca cacgtaccac ccgctgccgg
     1621 tggtcctgcg cgagggaagt ggcgtctggg tgaccgatgt cgacggccgt cgttacctcg
     1681 actgcctcgc gggctactcc gcgctgaact tcgggcacgg ccacccggtc atcgtggctg
     1741 cggctcgtga ccagctcgag cggttgacgt tgaccagccg cgccttccac cacgacctgc
     1801 tcgggtcgtt caccgccgag ctcgcgcaac tgtgcggcat ggaggcagtg ctgccgatga
     1861 acagcggcgc cgagggggtc gagagtgcac tcaagctagc ccgcaaatgg ggctacgagg
     1921 tcaagggcgt ccagccagac caggccaaca tcgtcgttgc gcggaacaat ttccacggtc
     1981 gcaccatcag cgtcgtgagc ttttccagtg accccgatgc gaagaacaac ttcggcccgt
     2041 tcacgcccgg ctttcgtgag gtcgccttcg gggacgccga cgcgcttgcg tctgcgatcg
     2101 acgtcaacac cgtcgctgtg ctcatcgaac caatccaagg tgaagcaggc gtcgtcattc
     2161 caccggtggg gtatctccgt gcggttcgcg acatctgtac ggctcgcggt gtgctgttca
     2221 tggccgatga gatccagtcg ggtcttggtc gcaccggtcg cacgttcgcg tgcgagcacg
     2281 aag
//
LOCUS       DATCBJ010000496         8497 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_49150, whole genome shotgun sequence.
ACCESSION   DATCBJ010000496 DATCBJ010000000
VERSION     DATCBJ010000496.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 8497)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8497)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8497
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_49150"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..876
                     /locus_tag="VII50_10095"
     CDS             <1..876
                     /locus_tag="VII50_10095"
                     /inference="COORDINATES: protein motif:HMM:NF016800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HEY5051240.1"
                     /translation="VRSTLWTLQDQRSLRFAGPFTYPSAVGLFFVMSLIAGTQILTGT
                     TDSRLRRIVNCVRAITVLGIVATDSRGAVIGLGVALCFRDVRRELGTAVVAAVIGAPL
                     LLLGQRTHTSHLLVIAAVVVAAALALIPDGVVHRVVRIVALPALCVVGWLLVTQHHAV
                     SGFDASWTERGHILRGAVTVFAKHPLLGAGPDPSFGAHTLTGQAGIAFFTHNEPVEIL
                     ISVGVVGAVVLVVCAVIVARALAAGRGSLAIPVLASVGAAGLVDFVWHFPALGLAAGI
                     VAGASGLRRASSVTP"
     gene            873..1505
                     /locus_tag="VII50_10100"
     CDS             873..1505
                     /locus_tag="VII50_10100"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020665018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEY5051241.1"
                     /translation="MSTPSIDTEQPLGELVLLRHGATEWSAGGKHTGRTDVPLSSLGE
                     RQAARVARIIAARSFALVLASPLRRAQETAALAGLSHIETDADLVEWDYGGYEGLTTS
                     QIRAILPGWSLWRDGVTAHGDGQPGENAADVGGRADRVIARVLPVLGHGDAALVSHGH
                     FLRVLAARWLSLPPTGGALLALDTASVSVLGFEHGAHVIRHWNMLAPAQT"
     gene            complement(1549..1776)
                     /locus_tag="VII50_10105"
     CDS             complement(1549..1776)
                     /locus_tag="VII50_10105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5051242.1"
                     /translation="MSSTLDPNVPLPPVDEDSDLVGENNKLAIPDDDPNSAEAPGVTL
                     PIAGAGSYPLPGVRFADELEGAVGEDEDKKK"
     gene            1906..2598
                     /locus_tag="VII50_10110"
     CDS             1906..2598
                     /locus_tag="VII50_10110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011718958.1"
                     /note="This protein belongs to the uracil DNA glycosylase
                     superfamily, members of which act in excision repair of
                     DNA. However, it belongs more specifically to UdgX branch,
                     whose founding member was found to bind uracil in DNA
                     (where it does not belong), without cleaving it, appears
                     to promote DNA repair by a pathway involving RecA, rather
                     than base excision; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UdgX family uracil-DNA binding protein"
                     /protein_id="HEY5051243.1"
                     /translation="MADEEYPGARAYVPAGADLDQVRAAVQMCRGCPLHAGTTQAVFG
                     AGPATARLVFVGEQPGDQEDRRGLPFVGPAGILLNRALSDAGIDREHAYVTNAVKHFK
                     FTQEGAGTRRIHQTPEQREVVACRPWLAAELRLIDPDVIVALGATAGKSLMGNDFRVT
                     RSRGTAIPWPPPGMAAADRRSILLVPTVHPSAVLRATDRDDAYAGLVADLEVAARALA
                     GVPAGRSGSDHT"
     gene            complement(2595..3266)
                     /gene="pdxH"
                     /locus_tag="VII50_10115"
     CDS             complement(2595..3266)
                     /gene="pdxH"
                     /locus_tag="VII50_10115"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: protein motif:HMM:NF004231.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HEY5051244.1"
                     /translation="MDSEQVAALRARYADIGLDEADLAVDPMTQFAGWLAEVVELGVP
                     EPNAMVLSTADADGRPSSRHVLLKGVGPDGFVFFTNYGSRKGQELAANPLASLCFPWF
                     CIGRQVVVTGRVTKVTRPESDSYFASRPRTSQLGAWASAHQSEVVANRAVLDDGYAQV
                     AAKYGEGDPIPTPPFWGGFRLAPATVEFWQGRNARLHDRLRFRRPDGLDGGVSGPWIV
                     ERLSP"
     gene            3372..4475
                     /locus_tag="VII50_10120"
     CDS             3372..4475
                     /locus_tag="VII50_10120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011718955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase 2"
                     /protein_id="HEY5051245.1"
                     /translation="MSDFVPGLEGVIAFETEIAEPDRDGGSLRYRGVDIEQLVGQTSF
                     GDVWGLLVDNSFTPGLPPFNTLVLPVRSGDVRVDVQAGVATLAPQLGFKPVLDLDDAQ
                     VRDELAQTSVTALSYVAQSARGDQTPVPHTEVEKGTTAVEQFLLQWRGELDPLQVKAL
                     DAYWVSAAEHGLNASTFTARVVASTGADVAAALSAAIGAMSGPLHGGAPTRVLHMVED
                     VEKSGDAEGYVKGLLDRHERLMGFGHRVYRAEDPRARVLRRVAQELGSPRFEAAAALE
                     HAALTELRTRRPDRPIETNVEFWAALILDLAAVPAHMFAAMFTCARVAGWSAHVLEEK
                     RTGRLIRPSARYIGPGTRPASSVPGWEVAHRHV"
     gene            4573..5706
                     /gene="serC"
                     /locus_tag="VII50_10125"
     CDS             4573..5706
                     /gene="serC"
                     /locus_tag="VII50_10125"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020641643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine transaminase"
                     /protein_id="HEY5051246.1"
                     /translation="MTDATESIEIPADLKPHDGRFGAGPSKVRPESVSALAAVSATYL
                     GTSHRRPGVKKVVGQVRAGLSDLFELPDGYEVVLGNGGATAFWDIATFGLIKERSQHL
                     SFGEFSSKFASAAKAAPFLGDPTVIKSEPGTHPLPQAEAGIDAYALTHNETSTGVMMP
                     INRIEGADADSLVLVDATSAAGGLPVDITQSDVYYFSPQKCFASDGGLWLAVFSPAAL
                     SRVEEIKASGRWVPTSYDLPTAIENSRLDQTYNTPALVTLWLLADQLEWMNSQGGLKF
                     TTSRTADSAQRLYTWAEKTDYTTPYVADPGMRSNVIGTINFDDSIDAELVAKTLRANG
                     IVDTEPYRKLGRNQLRIAMFPAIDPADVEALTTCIDYVVSKLS"
     gene            5781..6962
                     /locus_tag="VII50_10130"
     CDS             5781..6962
                     /locus_tag="VII50_10130"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY5051247.1"
                     /translation="MTYLQLAIYGYFLYAFAPSVTLLRDDEHTSRAISGLHGTGLAVG
                     AMLTAVIAPRLISRFGRARMIWVALGILSLGVFVFVSCDVVAVTITGALIAGFGGTLV
                     INIAVPILTAHHRGAAGGAAVTQANGLGSACGILAPLLVGGAVSIGIGWRAGVLVTVV
                     FAAVVAVVFGRRIRPDEIPAAPTQPHPHGARLSREYWRACFVLVMTNAIEFSMTIWSS
                     DVLNHHDGLSKGTAATGVTAIVTGMTIGRLSSGRVTLRYAADALLLFAFGVNIVGFGV
                     FWISTNPFLAFVGLFIAGLGISLQFPLAITRAVGFSDGRPDLATAYAALGGGFAVGAA
                     PFGLGALADHVGSHTAMTIVPLFVLLAAAGVATTRRPPDAASIAVAAHTAPVVIGDAT
                     A"
     gene            7350..8318
                     /locus_tag="VII50_10135"
     CDS             7350..8318
                     /locus_tag="VII50_10135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011718952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEY5051248.1"
                     /translation="MDYTHLGRTGLSVSRLCLGTMNFGPVTDVADSHDIMDRAHELGI
                     NFFDTANVYGWDDKGLTENIIGNWFSRGGGRREKTVLATKLYGDMAEWPNDGKLSALN
                     IRRACDASLKRLQTDYIDLYQMHHIDRAAPWDEVWEGYETLRAQGKIIYAGSSNFAGW
                     HIAKAQEAARRRNYLGLVAEQSLYNLAARTVELEVLPAALDYGIGVIAWSPLQGGLLG
                     GILRKQDNNRSKSGRADDTLAANRPAVEAYEAFCDELGEEPANVALAWLLHQPAVTAP
                     IIGPRTLAQLDGAVRAIEIKLDDKALARLDEIWPGPGGEAPEAYSW"
BASE COUNT         1340 a         2934 c         2793 g         1430 t
ORIGIN      
        1 gtccgctcga cgctctggac gttgcaggat cagcgttcat tgcggttcgc cgggcctttc
       61 acctacccaa gcgcagtcgg tctgttcttt gtcatgtcac tcattgctgg cacccaaatc
      121 cttaccggta cgacggattc gcgacttcgc cggatcgtca actgcgtgcg cgcgatcacc
      181 gttctcggca tcgtcgcaac cgacagccga ggcgccgtga tcgggctggg ggtcgcgttg
      241 tgctttcgcg acgtccgccg agaactcggt actgccgtgg ttgcggcggt catcggcgcg
      301 ccactactgc tgttaggtca gcgcacgcac acgagccatc tgcttgtgat cgcggcggtg
      361 gttgtcgctg ccgctcttgc cttgataccg gacggcgtgg tgcaccgcgt cgtgcgcatc
      421 gtcgctcttc cggcgttatg cgtggtcggt tggctgctcg tcacgcagca ccacgccgtc
      481 tcgggattcg atgcgagttg gaccgaacgc ggtcacatcc tgcgcggcgc ggtgaccgtc
      541 ttcgccaagc atccgctgct cggcgcagga cccgacccga gctttggcgc gcacaccttg
      601 accggtcaag cgggcattgc gtttttcacc cacaacgaac cggtggagat cctgatttcg
      661 gtcggcgtag tcggcgcggt cgtgctcgtc gtgtgtgccg tgatcgtcgc ccgcgcactt
      721 gccgcaggcc gcggatcgct ggcgattccg gtgcttgcca gcgtcggcgc agccggactt
      781 gttgacttcg tgtggcactt ccccgcactc ggactggccg ccggaatcgt ggccggcgcg
      841 agcggattgc gacgcgccag tagcgtgacg ccgtgagcac gccctcaatc gacacagagc
      901 agcctctcgg tgagctcgtg ttgctccggc acggcgccac cgaatggagc gctggtggca
      961 aacacaccgg tcgcaccgac gtcccgctca gctcgctcgg cgagcggcag gctgcgcggg
     1021 tggcccgcat catcgccgcc cgtagcttcg ccctcgttct cgccagcccg ttgcgtcgcg
     1081 cgcaggaaac cgcagcgctc gccgggttgt cgcacatcga gaccgacgcc gaccttgtcg
     1141 agtgggacta cggcgggtac gaaggtctga cgacgtcaca gatcagagcg atcctgcccg
     1201 gctggtccct atggcgggac ggcgtgaccg cccatggtga cggtcagccc ggcgagaatg
     1261 cggcggacgt cggggggcgg gccgatcggg tcattgcgcg ggtgttgccg gtgctcggtc
     1321 acggcgatgc ggcgctggtc tcgcacgggc acttcttgcg cgtactcgcg gcccgctggc
     1381 tgtcgttacc gccaacgggc ggggcgctgc tcgcgctcga cacggcaagt gtgtcggtgc
     1441 tcggcttcga gcacggcgcg cacgtgatcc ggcactggaa catgttggcg ccggcacaaa
     1501 cgtgacggtt gctgccccct gggggcagca aactcccacg atttgggctt acttcttctt
     1561 gtcctcgtcc tcgccgacgg caccttcgag ttcgtccgcg aagcgcacgc ccggcagcgg
     1621 gtacgaaccg gcgccggcga tcggcaacgt cacacctggc gcctccgcac tgttcgggtc
     1681 gtcgtcgggg atggcgagtt tgttgttctc gccgaccaga tcggagtcct cgtcaactgg
     1741 cggcaagggc acgtttgggt caagggttga gctcatggtc gcactcctcg aactcctcaa
     1801 gcggccgcct cgcgcggcgg gtcgatgcag gtcttaccct gcctgcagca gcgaggaacc
     1861 ctcctgggta agggaacatc ggcgactgac gcggcaggag ggctagtggc cgacgaggag
     1921 tatccggggg cgcgggcgta cgtcccggcg ggcgccgacc tcgaccaggt gcgcgcggcc
     1981 gtacagatgt gtcgcggctg cccactgcac gccggcacga cgcaggccgt cttcggagcg
     2041 ggaccggcga cggcacggct ggttttcgtc ggcgagcaac ccggtgacca agaagaccga
     2101 cgcggcctgc cgttcgtggg tcccgcaggg atcctgctga atcgcgcgtt gagtgacgcc
     2161 ggcatcgacc gtgaacacgc gtacgtgacc aacgcagtga agcatttcaa gttcacccag
     2221 gaaggcgccg gtacgcgccg catccatcag accccggagc agcgcgaggt cgtcgcgtgc
     2281 cgcccgtggc tggccgccga actgcgactg atcgaccccg atgtcatcgt cgcgctcggc
     2341 gcgacggccg gaaaatcctt gatgggtaac gattttcggg taaccaggtc gcggggaact
     2401 gccatcccgt ggccgccgcc cggcatggcc gccgccgatc gccggagcat cctgctcgtg
     2461 ccgacggtcc acccgtcggc ggtgcttcgg gccaccgacc gcgacgatgc ctacgccggt
     2521 ctggtggccg atctcgaagt ggccgcacgc gcacttgccg gcgtacccgc gggccggtcg
     2581 ggttcagacc acacctaagg cgacaggcgc tcgacgatcc acggcccgga caccccgccg
     2641 tccaggccgt ccgggcgccg gaagcgcagc cgatcatgca ggcgcgcatt ccggccctgc
     2701 cagaactcca cggtcgccgg cgcgagccga aaaccacccc agaatggcgg ggttgggatt
     2761 gggtcgccct cgccgtactt ggccgcgacc tgcgcgtacc catcgtcgag caccgcacgg
     2821 ttcgcgacga cctccgattg atgcgcgctt gcccacgcgc ccagctgcga agtgcggggt
     2881 cgcgacgcga agtaggagtc cgattcgggc ctcgtgacct tggtcacacg accggtcacg
     2941 acgacctggc gtccgataca aaaccaggga aagcacaggc tggccaacgg attcgccgcc
     3001 agctcctgac cttttcgaga gccatagttg gtgaagaaga cgaacccgtc aggcccgaca
     3061 cctttgagca gcacatgacg actgctcggt cgcccatcgg cgtccgccgt ggacagcacc
     3121 atcgcattgg gctccggaac gccgagctcg accacctcgg cgagccaccc cgcgaactgg
     3181 gtcatcggat cgaccgcaag atcggcctcg tccagcccga tatctgcgta gcgcgctcgc
     3241 agcgcagcca cctgctcgga gtccattttc aagcacctcg gcattctccg caggacctgc
     3301 tcgttcaacc cgcacagtag cggggacaat gtgaagaccg ccgcgcatga tcagagcgag
     3361 gagtgccgct gatgtctgac ttcgtcccag gcctcgaagg ggtcatcgcc ttcgagaccg
     3421 agatcgccga gcccgacagg gacggcggat cgctgcgcta ccgcggcgtc gacatcgagc
     3481 aactggtcgg ccagacgtcc ttcggcgacg tgtggggact gctggtcgac aactcgttca
     3541 cgcccgggct gccgccgttc aacacactgg tgttgccggt gcgctccggc gacgtgcggg
     3601 tcgacgtcca ggctggggtg gcgaccctcg ccccgcagct cggcttcaag ccggtcctcg
     3661 acctcgacga cgcccaggtt cgcgacgagc tggcccagac ctcggtgacc gcgctctcct
     3721 atgtcgcgca gtccgcgcgc ggcgaccaga cgcccgtgcc gcacaccgag gtcgagaagg
     3781 gcaccaccgc ggtcgagcag ttcctgctgc agtggcgcgg cgagctcgac ccgctccagg
     3841 tgaaggcact ggacgcctac tgggtgtcgg ccgccgagca cgggctcaac gcgtccacct
     3901 tcaccgcacg agtggtggcg tcgaccggcg ccgatgtcgc ggccgcactc tcggcagcga
     3961 tcggggcgat gtctggcccg cttcacggcg gtgctccgac gcgggtgctg cacatggtcg
     4021 aggacgtcga gaaaagcggc gacgctgagg gctacgtgaa gggcctgctg gatcgccacg
     4081 agcggctgat gggtttcggc caccgggtct accgcgccga ggacccacgt gcccgggtgt
     4141 tgcggcgggt ggcccaagaa ctcggatcgc cgcggttcga ggcggcggcc gcgctggagc
     4201 acgcggcgct caccgaactg cgcacccgac gtccggaccg cccgatcgag accaacgtcg
     4261 agttctgggc ggcactgatc ctcgacctgg ccgcggtccc cgcgcacatg ttcgcggcga
     4321 tgttcacctg cgcacgtgtc gcgggctgga gcgcgcacgt gctcgaggag aagcgcaccg
     4381 gacgcctgat caggccgtcg gctcgctaca tcggcccggg cacccgcccc gcgtcgtcgg
     4441 tcccaggctg ggaagtagcc caccgccacg tgtgaggccc gccgcccggg cgggagcgca
     4501 cccgtggacc tgcgacaatg agaaccggca gcgtcgccga tcccacccgt gcagctttta
     4561 gggagccctc ccgtgaccga cgcgaccgag agcatcgaga ttccggccga cctcaaaccc
     4621 cacgatggcc ggttcggcgc cggcccctcc aaagtccggc ccgaatcggt gtccgcgctt
     4681 gccgcggtca gcgcgaccta cctgggcacc tcgcaccggc gtccgggcgt caagaaggtc
     4741 gtcggccagg tccgggccgg gctcagcgac ctgttcgaac tgcccgacgg ctacgaggtg
     4801 gtcctcggca acggcggcgc gaccgcgttt tgggacatcg cgaccttcgg cctcatcaag
     4861 gagcgctcac agcacctaag cttcggtgag ttctccagca agttcgcgag cgcggccaag
     4921 gcggcgccgt tcctcggcga cccgacggtc atcaaatccg agcccggcac gcacccactc
     4981 ccgcaggccg aagcgggcat cgacgcctac gcgctcaccc acaacgagac gtcgaccggc
     5041 gtcatgatgc cgatcaatcg gatcgaaggc gcggacgccg attcgctggt gttggtcgac
     5101 gccacttctg ctgctggtgg tctgccggtc gacatcacgc agtccgacgt gtactacttc
     5161 tcaccgcaga agtgcttcgc ctccgacggc ggcctgtggc tcgcggtctt cagcccggca
     5221 gctctgtccc gcgtcgagga gatcaaggcg tccgggcgct gggtcccgac gtcctacgat
     5281 ctcccgaccg cgatcgagaa cagccggctc gaccagacct acaacactcc cgcactggtc
     5341 acgttgtggt tgctcgccga ccagctcgaa tggatgaact cgcagggcgg tctgaagttc
     5401 acgacgtccc gtaccgctga ctccgcgcag cggctctaca cctgggcgga gaaaaccgac
     5461 tacacgacgc cgtatgtggc cgatcccggc atgcgttcca acgtcattgg cacgatcaac
     5521 ttcgacgact cgatcgacgc cgaactggtc gcgaaaacgc tgcgggctaa cgggatcgtt
     5581 gacaccgagc cctatcgcaa gctcggccgc aaccagctgc gaatcgcgat gttccccgct
     5641 atcgatcccg cagatgtcga agcactgacg acctgcatcg actacgtcgt gtcgaagctc
     5701 tcctgacgaa ggacaccccc actaacccgg acgccgacac tgcggcgtcc gggcgcctcg
     5761 tacgtgatcg cctgacctgg atgacctatc tgcaactggc gatctacgga tacttcctgt
     5821 acgccttcgc gccatcggtg acgctgctgc gcgacgacga gcacaccagc cgcgcgatct
     5881 ccggtttgca cggaaccggc ctcgccgtcg gtgcgatgtt gacggccgtg atcgccccaa
     5941 ggctgatctc gcgcttcggc cgcgcgcgga tgatctgggt ggcgctcggg atcctgtcgc
     6001 tcggcgtctt cgtgttcgtt tcgtgcgacg tcgtcgccgt cacgattacg ggagcgttaa
     6061 tcgccggctt cggcggaacg ctggtcatca acatcgcggt cccgatcttg accgcccatc
     6121 accgcggcgc cgcggggggt gcggcggtaa cccaggcaaa cgggctcggg tcggcctgcg
     6181 gcattctcgc accgttactc gtcggcggtg ccgtatcaat cgggatcggc tggcgtgccg
     6241 gcgtcctcgt gacggtggtg ttcgcggccg tcgttgccgt ggtcttcggc cgccgcatcc
     6301 ggcccgacga gattcctgcg gcacctacgc agccgcaccc gcacggcgcc cggctatcgc
     6361 gcgaatactg gcgcgcgtgt ttcgtgctcg tcatgacgaa cgccatcgag ttctcgatga
     6421 cgatctggag ttccgacgtc ctcaatcacc acgacgggct ctccaaaggc acggccgcaa
     6481 ctggagtgac cgcaatcgtg accggcatga cgattggccg gctgtcgtcg gggcgcgtca
     6541 ccctgcgcta cgccgccgac gcgctgttgc tgttcgcctt cggcgtcaac attgttgggt
     6601 tcggcgtctt ctggatatcg acgaacccgt tcctggcatt cgtcgggctg ttcatcgccg
     6661 ggctcggcat ttcgctgcag ttcccacttg cgatcacgag ggcggtggga ttctccgacg
     6721 gccgccccga tctggcaacg gcctacgcgg cgctgggcgg cgggttcgcc gtcggtgccg
     6781 cgcccttcgg actcggcgcg ctcgccgacc acgtcggatc acacaccgcg atgaccatcg
     6841 tcccgctctt cgtgttgctg gccgctgcgg gcgtagcgac cacgcggcgt ccgcctgacg
     6901 cggccagcat cgccgtcgcc gcacacacgg cgcccgttgt tatcggcgac gccacagcct
     6961 gaaaccggcc gggccccagg gccaccaagc gaagcggtgc ttcagctatt tggcgaccga
     7021 tcattcggcc gatgcggtca tcgcaggtgt cgacacgacg gtgggagcgg gaaatcgctg
     7081 gtgtcgggtg ccccgagtca gccacccgct gtcgagcagg acgcgaagca caccagtgcg
     7141 actggagttt cgctgtgcgt gttccacggc agcttcgacg cgccgggatt cgtggcgacg
     7201 cgcacctacg tcatggtcgg cctcgacggg acgtcgctct acccgtctgg ttcggaacga
     7261 tcagacctcg gtgcctgctc agccgggttc ctagcgcctg gccgggcacc gccaaccggc
     7321 gtccggctcc ggcgtgaaat gattgagaga tggactacac acatcttggc cgcaccggcc
     7381 tctccgtcag ccgcctctgc ttgggcacca tgaacttcgg tccagtgacc gacgtcgccg
     7441 acagccacga catcatggac cgcgcccacg agctcgggat caacttcttc gacactgcga
     7501 acgtgtacgg ctgggacgac aagggactca ccgagaacat catcggcaac tggttctcgc
     7561 gcggcggcgg ccgtcgggag aagactgttc tcgccaccaa gctgtacggc gatatggcgg
     7621 agtggccaaa cgacgggaag ctgtcggcgc tgaacatccg ccgcgcgtgc gatgcgtcac
     7681 tcaagcgcct gcagaccgac tacatcgacc tctaccagat gcaccacatc gatcgcgcgg
     7741 cgccctggga cgaggtctgg gagggctacg aaacccttcg tgcgcaagga aaaatcatct
     7801 acgccggctc atcgaacttc gccggctggc acatcgccaa agcgcaagag gctgcccgtc
     7861 ggcgcaacta cctcggcttg gtcgcggagc agtcgctgta caacctcgct gcgcgcacgg
     7921 tcgaactcga agtcctccct gcagcgctcg actacggcat cggcgtcatc gcgtggagtc
     7981 cgctgcaagg cggtctgctc ggcggcatct tgcgcaagca ggacaacaac cgcagcaaga
     8041 gtggccgcgc agacgacaca ctcgcggcga accggccagc cgttgaagcc tacgaggcct
     8101 tctgcgacga gctcggcgaa gagcccgcga acgtcgcgct cgcgtggctg ttgcatcagc
     8161 ctgcggtgac ggccccgatc atcgggccgc ggacgttggc gcagctggac ggcgcggtgc
     8221 gggcgatcga gatcaagctc gacgacaagg cgctcgcccg gctcgacgag atctggcccg
     8281 gcccgggcgg cgaggcccca gaggcctact cctggtaagc agtacccatg agcgagttgg
     8341 cggcggcaca ggctgtccac ctgccctacg gacgggtgcg cgaccagcac ggccgcgaga
     8401 tcgcgggcgt tcggctgggt gctgacctcg ttgatctagc tgctttcttg gacggcggcg
     8461 tgctcgccgg tctgttcact gcggctcggc tcgagcc
//
LOCUS       DATCBJ010000497         2274 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_375742, whole genome shotgun sequence.
ACCESSION   DATCBJ010000497 DATCBJ010000000
VERSION     DATCBJ010000497.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2274)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2274)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2274
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_375742"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(80..670)
                     /gene="cysC"
                     /locus_tag="VII50_10140"
     CDS             complement(80..670)
                     /gene="cysC"
                     /locus_tag="VII50_10140"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: protein motif:HMM:NF003013.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004020 - adenylylsulfate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HEY5051249.1"
                     /translation="MSTDSSVDTDVLAGSSTDRRRGATVWLTGLPSAGKSTIARALAS
                     TLRADGYHVELLDGDELRTHLTADLGFSREDRDTNVRRVGFVAGLLARNGVIALVPVI
                     APYAAARAAVRADHDIDGTAFVEVHVATPLELCAERDVKGLYAKQRAGEIRGLTGVDD
                     PYEIPQDPELRLDTQGLSVEESAALVRAALAARGIA"
     gene            complement(667..1395)
                     /locus_tag="VII50_10145"
     CDS             complement(667..1395)
                     /locus_tag="VII50_10145"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010078847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004604 - phosphoadenylyl-sulfate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0019379 - sulfate assimilation,
                     phosphoadenylyl sulfate reduction by
                     phosphoadenylyl-sulfate reductase (thioredoxin) [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenylyl-sulfate reductase"
                     /protein_id="HEY5051250.1"
                     /translation="MSGTTAGPPVAELDRLAALERLATDAARDLVDASAHEILQWAAQ
                     TFGDRFCVLSSMGDAVVASLAAKAKPGVDVVFLDTGYHFAETIGMRDAVAAVYDVNVV
                     SALPLLTVAEQDDAYGKDLFARDPDQCCAMRKVAPMEAALLNYDSWATGVRRDESSTR
                     ATTPAVQWDARRRKVKVNPIARWSRDDVDTYIADNNILVSPLIDDGYLSIGCWPCTSK
                     VAPGADPRSGRWVGHAKTECGINT"
     gene            complement(1392..1595)
                     /locus_tag="VII50_10150"
     CDS             complement(1392..1595)
                     /locus_tag="VII50_10150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5051251.1"
                     /translation="MTSGDASDGVVAGVGDSDVGAGERATPFYCPYCGDEDLRPHGDT
                     GGSWHCRGCARVFTLKFVGLEVR"
     gene            complement(1592..>2274)
                     /locus_tag="VII50_10155"
     CDS             complement(1592..>2274)
                     /locus_tag="VII50_10155"
                     /EC_number="1.8.7.1"
                     /inference="COORDINATES: protein motif:HMM:NF013259.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0050311 - sulfite reductase (ferredoxin)
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="nitrite/sulfite reductase"
                     /protein_id="HEY5051252.1"
                     /translation="DGRRDHVGVHPQKDGQFYVGFAPRVGRLSGTKLGQIADLAQAHG
                     SGRVHATVEQKMIVLDVPADQVESLAAGLEALDLQVNPSTFRRQTMACTGIEFCKLAI
                     VETKNRAMTLIDELERRLPEFDSPMTINVNGCPNSCARFQVADIGLKGSLVTNAAGEQ
                     VGGYQVHLGGSLGVDANFGRKLRGLKVTEDELEDYVERLANRYLEQRGDGERFTQWVH
                     RADEADLQ"
BASE COUNT          369 a          791 c          723 g          391 t
ORIGIN      
        1 acgtcgaggt gcggcaaccg cagcacaccg ttggtctcgg cagtcggctg cggcttgaac
       61 accgtgccgt caaccgtggt catgcgatac cccgcgcagc taacgcagca cgcaccagcg
      121 ccgccgactc ttcgacgctc aacccttgcg tgtcgagccg aagctcagga tcctgcggga
      181 tctcatacgg atcgtcgacc ccggtgagtc cacgtatttc gccggcgcgc tgctttgcgt
      241 acagaccttt gacgtcgcgc tccgcgcaca actcgagcgg cgtcgccaca tgcacctcga
      301 caaaggcggt gccatcgatg tcgtgatcgg cccgcaccgc cgctcgtgca gccgcgtagg
      361 gcgcgatcac cgggaccagg gcgatgacgc cgttgcgcgc gagcaacccg gcgacgaatc
      421 caacccggcg cacgttggtg tcgcggtcct cgcggctgaa gcccaggtcg gcggtgaggt
      481 gcgtccgtag ctcgtcgccg tcgagcagct cgacgtggta cccgtcggcg cgaagcgtcg
      541 aggcgagagc gcgggcgatg gtcgacttgc ccgcgctcgg caaaccggta agccacaccg
      601 tcgcgccgcg acggcggtcg gtcgacgaac ctgccagcac atcggtgtcg acactgctgt
      661 cggtgctcac gtgttgatcc cgcactcggt tttcgcgtgc ccgacccagc ggcccgacct
      721 cgggtctgca cccggggcaa ccttgctggt gcacggccag cacccgatcg acaggtaacc
      781 gtcgtcgatc agcgggctca ccagaatgtt gttgtcggcg atgtaggtgt cgacgtcgtc
      841 gcggctccag cgcgcgatcg ggttgacctt gaccttgcgg cgccgcgcat cccattgcac
      901 ggccggcgtc gttgcacgcg tcgaagactc gtcacgccgg acgccggtag cccaagagtc
      961 gtaattcagc agggccgcct ccatcggggc aaccttgcgc atcgcgcagc actgatcggg
     1021 gtcacgggcg aagagatcct tgccgtacgc gtcgtcttgt tcggcgaccg tcagcagcgg
     1081 caatgccgac accacgttga cgtcgtagac agccgcgacc gcgtcgcgca tcccgatcgt
     1141 ttcggcgaag tggtagccgg tgtcgaggaa gacgacgtcg acgccgggct tggccttggc
     1201 ggcaagcgac gcgaccactg cgtcgcccat cgatgacaac acgcagaacc gatctccgaa
     1261 cgtctgcgcc gcccactgca ggatctcgtg cgccgatgcg tcgacgagat cgcgcgccgc
     1321 gtcagtagct agtcgttcga gggcggctag gcggtcgagc tcggcgaccg gcggaccggc
     1381 agtcgtcccg ctcatcgcac ctccagcccg acgaatttca atgtgaagac gcgagcacat
     1441 ccgcggcagt gccatgagcc ccctgtgtcc ccgtgcgggc gcaggtcttc gtcgccgcag
     1501 tacgggcagt aaaacggcgt cgcgcgctca ccggcaccga cgtcggagtc gccaacgccg
     1561 gccacgacac catcggacgc gtccccgctt gtcattgcag gtccgcttcg tctgcacgat
     1621 gcacccactg cgtgaagcgc tcaccgtcgc cgcgttgttc caggtagcgg ttcgcgagtc
     1681 gctcgacgta gtcctcgagc tcgtcctcgg tgactttgag tccacgtagc ttgcggccga
     1741 agttcgcgtc gacaccgagg ctgccgccga ggtgcacctg atacccgcca acctgctcac
     1801 cagcagcatt ggtgaccagc gatcccttca aaccgatgtc ggcgacctgg aatcgcgcgc
     1861 acgagttcgg gcagccgttc acgttgatcg tcatcggact gtcgaactcc ggcagccgcc
     1921 gctcgagctc gtcgatgagc gtcatcgcgc ggttcttcgt ctcgacgatc gcgagcttgc
     1981 agaactcgat ccccgtgcac gccatggtct gacgccggaa cgtgctcgga ttcacctgga
     2041 ggtcgagtgc ctccagcccc gcggccaacg actcgacctg atccgctggc acgtcgagca
     2101 cgatcatctt ctgctcgact gtcgcgtgga cacgaccgct gccgtgagcc tgcgcgaggt
     2161 cggcaatctg gcccagtttg gtgccgctca gtcggccgac tcgcggcgcg aaaccgacgt
     2221 agaactggcc atccttctgc ggatgcacgc cgacgtggtc acgtcgtccg tcgg
//
LOCUS       DATCBJ010000498         2531 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_185973, whole genome shotgun sequence.
ACCESSION   DATCBJ010000498 DATCBJ010000000
VERSION     DATCBJ010000498.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2531)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2531)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2531
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_185973"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..510)
                     /locus_tag="VII50_10160"
     CDS             complement(<1..510)
                     /locus_tag="VII50_10160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5051253.1"
                     /translation="MAIGLVFGTTVLSSLFFHSRVKVVDQTVHAELATVTQLVTADAL
                     PAPLPSAPGGTAFAQVLDAADTVLASTTSASTVLPLLPLPQFSHRLGHAFTTTGSSLG
                     TTPMRVEAQTASLHGVPVTVLVAVPFADVTSALDALRNVVVIVVPLVLLAAAAATWLA
                     VGSALRPVDD"
     gene            complement(576..1334)
                     /locus_tag="VII50_10165"
     CDS             complement(576..1334)
                     /locus_tag="VII50_10165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020664352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEY5051254.1"
                     /translation="MELLSAATRRPAPLSSSALSIVTASPRYGIVTAMRLLIIEDEHR
                     MAAAMQRGLVHAGFTVDVAGDGITGLEMATAVGYDVIVLDVMLPGLSGYEVTRQLRAR
                     EIWTPILLASAKDGDYDQADGLDLGADDYLTKPFSFVVLLARIRALLRRGASGPRPSV
                     LVAAGLTLDPAARTVSKGDEPIDLTAREFAMLEYLMRQHPLPVTKAELLEHVWSDATT
                     EANAVEVYAGYLRRKVGRDVVQTIRGVGYRLRAT"
     gene            1333..>2531
                     /locus_tag="VII50_10170"
     CDS             1333..>2531
                     /locus_tag="VII50_10170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5051255.1"
                     /translation="MTRLASRRLRWLLPAGIAAAIAVASVATTAAANASAEPKLAAKT
                     AAQLLVAVENASPAGLSGTIVETAKLGLPDISGLVGSTGGSGLSLETLVTGSNTMNVW
                     YAGSDRQRFALLGQLSEDDVIHNGTDLWSYDSSTRTVTHKTLTQNASDKVGPSIASAA
                     PSLDPQTAAEKALAAIDPTTVVAVDRTARVAGRAAYQLVLSPKDTRSLIGSVQIAIDA
                     ATSMPLRVQIFAAGATSPAFQVGFTSVSYNVPNASVFEFVPPAGASVEQDTAPFNDSL
                     SDSAFGNNSFVIHTRVRGSAAGPQQTFVVGGSAVSTNVTPNRKVTELGKGWTEVIKIA
                     NAMPALPKDVPLPSDGPAPSSGPDGGPLITVQRHSALNDIATPVPGGELITTALVSIF
                     IANDGTA"
BASE COUNT          460 a          838 c          810 g          423 t
ORIGIN      
        1 gtcatcgacc gggcgtagcg ccgacccgac ggcgagccac gttgcggcgg cagcagcaag
       61 gagcacgagc ggcaccacaa tcacgaccac attgcgcaat gcatcgagcg ccgacgtgac
      121 atcagcgaac ggtacagcga caagcacagt caccgggacg ccgtgcagcg acgctgtctg
      181 tgcctccacg cgcatcggtg tcgtaccgag agagcttccc gtcgtcgtga atgcgtgacc
      241 gagtcgatgc gagaactgcg ggagcggcaa caacggcaag actgtgcttg ctgacgtcgt
      301 cgaggcgagc accgtgtcag cggcatcgag aacctgcgcg aacgcagtgc cgccgggcgc
      361 cgacggcagc ggagccggaa gggcatcggc cgtcaccagc tgcgtgacag ttgcgagctc
      421 ggcgtgcacg gtctggtcga ccactttcac tctgctgtga aagaacaacg agctcagcac
      481 cgtggtgccg aaaaccagtc cgatcgccaa gactaaagtc gccgttgcgg tcacgcgcag
      541 tcggagcgtc cggcgagccc accacttcaa cggattcacg tcgcgcgcag ccggtagccg
      601 acacctcgaa tggtctgcac gacgtcgcgg ccgaccttgc ggcgtaagta gcccgcgtac
      661 acctcgaccg catttgcctc cgtggtcgcg tcgctccata catgttcgag cagctccgcc
      721 ttggtgacgg ggagcggatg ttgtcgcatc aggtattcga gcatcgcgaa ctctcgcgcg
      781 gtgaggtcga tcggttcgtc gcccttgctc accgttcgtg cggcgggatc gagagtgagg
      841 ccagcagcta ctaacacgga cggtcgcggg cccgaagcgc ctcgacgtag caacgcgcga
      901 atgcgagcga gcaatacaac gaaggagaac ggtttggtga ggtagtcgtc ggcaccgagg
      961 tcgagcccat ccgcttggtc gtagtcgccg tccttcgccg acgcgagcag gatcggcgtc
     1021 catatttctc gtgctctgag ttgtcgcgtg acttcgtacc cggacagtcc cggcagcatc
     1081 acgtccagca cgatcacgtc gtaaccgacc gccgtcgcca tctcgagccc ggttatcccg
     1141 tcgcccgcga catcgacggt gaagcccgca tgcaccagcc cgcgttgcat cgcggcggcc
     1201 atgcggtgct catcctcgat gatcagcaat cgcatagcgg tcacgatgcc atagcggggc
     1261 gaagctgtga cgatactgag cgccgaggac gacaaaggtg cgggacggcg ggtggcggcc
     1321 gatagtaatt ccatgacacg cctcgcttcg cgccgcctcc gctggcttct gccggcaggt
     1381 atcgccgccg ccattgccgt cgcctccgtc gccactactg ctgcggccaa cgcgtcggcg
     1441 gaacccaagc tggccgccaa gacagctgcc caactgctcg tcgctgtcga gaacgcgtcg
     1501 cccgctggac tttcgggcac gatcgtcgag acggcgaaac tgggtttgcc ggacatctcc
     1561 ggattggttg gttccactgg cggctcgggc ttgtcccttg agaccctggt gacgggttcc
     1621 aacacgatga acgtgtggta cgccgggtcg gaccgtcagc ggttcgcgtt gctcgggcag
     1681 ctttccgagg acgacgtcat tcacaacggt accgacctgt ggagctacga ctcgagtaca
     1741 cgaacggtca ctcacaagac cttgacgcag aatgcgtccg acaaggtcgg accgtcgatc
     1801 gctagcgctg cgccgtcgct cgatccgcag acggccgccg aaaaggcgct tgccgcgatc
     1861 gacccgacga ccgtcgtggc tgtcgatcgc acggcgcgag ttgctggtcg cgccgcgtac
     1921 caactggtgc tttcgcctaa ggacacccgg tcgctcatcg gcagcgtgca gatcgcgatc
     1981 gacgctgcga cgtccatgcc gttgcgggta cagatcttcg cggccggcgc gaccagcccg
     2041 gcgttccagg tcggcttcac cagtgtcagc tacaacgtgc ccaacgcgtc ggtcttcgag
     2101 ttcgtgccgc ccgctggtgc gagcgtcgag caggacaccg cgccgttcaa cgacagcctg
     2161 agcgacagcg ctttcggcaa caattctttc gtcatccaca cccgcgttcg tggcagcgcc
     2221 gctggtccgc agcagacctt cgtcgtcggg ggcagcgctg tttccaccaa cgtgactccg
     2281 aaccggaagg tgaccgaact cggcaagggc tggaccgagg tgatcaagat cgccaacgcg
     2341 atgccggcgc tgccgaagga cgtcccgctg ccatcggacg ggccggcacc ctcgagcggg
     2401 ccggacggcg ggccgctgat caccgtgcag cggcattcgg cactgaacga catcgcgaca
     2461 ccggtccctg gcggcgagct gatcacgacc gcactcgtgt cgatcttcat tgcgaacgac
     2521 ggcacggcgt a
//
LOCUS       DATCBJ010000499         2395 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_159608, whole genome shotgun sequence.
ACCESSION   DATCBJ010000499 DATCBJ010000000
VERSION     DATCBJ010000499.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2395)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2395)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2395
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_159608"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..428
                     /locus_tag="VII50_10175"
     CDS             <1..428
                     /locus_tag="VII50_10175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="peptidoglycan-binding protein"
                     /protein_id="HEY5051256.1"
                     /translation="QAAVKVGDPVTITLPDTSTTPGVVSTVGTVATSAAGNNPGQAST
                     PTIEVDVTPTDPAATGALDQAPVLVAITTASVTGALVVPVAALLALTGGGYAVEVIAA
                     DGTHQLVGVTLGLFDDADGLVQISGSAVHAGSRVVVPGS"
     gene            425..1123
                     /locus_tag="VII50_10180"
     CDS             425..1123
                     /locus_tag="VII50_10180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009212035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY5051257.1"
                     /translation="MTRVLELEDVSKVYPGHPPVTALDGVSFSVTAGELVGVVGPSGS
                     GKTTLLHLIGTLDRPTSGTVRITGMDVALLSDRELSRLRGARIGFVFQQFFLAEHATI
                     LDNVADGLLYAGVALRERRAEAIEALEQVGLGDRITARPTQLSGGQRQRVAIARALIG
                     RPAILLADEPTGNLDTATGQAILGLLEDLHAAGTTIVVITHDRDIAARMPRQIQMRDG
                     RIVADTALSAASSL"
     gene            1120..2328
                     /locus_tag="VII50_10185"
     CDS             1120..2328
                     /locus_tag="VII50_10185"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY5051258.1"
                     /translation="MTTAALSHATGRLRFTDLARLSTIGLRTRKLRATLSALGIAIGV
                     AAVVAVLGLSASSQAGLLAEIDKLGTNLLTVTNGQTVFGQNAELPVAAPGMISRIGPV
                     TSVQATGSVTASVYRSPLIPAIQTNALSVLASTLDLPATVGTTLAAGSYLNAATATEP
                     VAVLGSSAASRLGIDRIYPGERIWAGNQWFYVVGILNPAVLAPELDAAMLVGFPAAKT
                     YLGFDGHPSTIYLRARTDQVAAVQAVLAATADPEAPNQVNVSQPSAALVARAETKSAL
                     NGLFLGLGAVALLVGAIGVANIMVISVLERRSEIGLRRALGATKGHIRTQFLSEAIVL
                     AAIGGAAGVGAGALATAIYASTKSWAIVVPPIAWGGSLGAALLIGAVAGLLPALRAAR
                     MPPTRALWSM"
BASE COUNT          378 a          837 c          787 g          393 t
ORIGIN      
        1 agcaggccgc cgtcaaggtc ggcgacccgg tgacgatcac gttgcccgac acgagtacga
       61 ctccgggtgt ggtgtcgacg gtggggacgg tcgcgacctc cgccgcgggc aacaaccctg
      121 gacaagcgtc gacgccgacg atcgaagtcg acgtcacgcc gaccgacccg gccgcgaccg
      181 gcgcgctgga ccaggctccg gtgctggttg cgatcaccac cgcgagcgtg accggtgcgc
      241 tggtagttcc ggtggcggca ctgctcgcac tcactggtgg cggctacgcc gtcgaagtca
      301 tcgccgccga tggcacgcac caactcgtcg gcgtcacgct cggcctgttc gatgatgctg
      361 atgggctggt gcagataagc ggttctgcag tgcacgccgg gtcgcgagtg gtggtgcccg
      421 ggtcgtgaca cgtgtccttg aactggaaga cgtcagcaag gtctatcccg gccacccacc
      481 ggtgaccgca ctggacggtg tcagcttcag cgtgaccgcc ggcgagcttg tcggcgtcgt
      541 cggcccatcc gggtctggga agacgacgtt gttgcatctg atcggcacgc tggatcgccc
      601 tactagtggc acggtgcgca tcaccggcat ggacgtcgcg ttgctgtccg accgggaact
      661 gtcgaggttg cgcggggcca ggatcggatt cgtgttccag cagttcttcc tcgccgaaca
      721 cgcaaccatc ttggacaacg tcgccgacgg gttgctgtac gcgggcgttg cgctgcgtga
      781 gcgcagggca gaggcgatcg aggcactcga acaggtcggg ctcggcgacc ggatcaccgc
      841 ccggccgacc caactgtcgg gtgggcaacg gcagcgggta gcgatcgcgc gcgccctgat
      901 cgggcggccc gcgatcctgc ttgcggacga acccaccggc aacctggaca ccgcgaccgg
      961 acaggcgatc ctcggactac tcgaagacct gcacgcggcc ggcaccacga tcgtggtcat
     1021 cacccacgac cgcgacattg cggcacggat gccacgacag atccagatgc gcgacgggcg
     1081 catcgtcgcc gacaccgcac tgtcggcagc gagttcgctg tgacaaccgc tgcactctcg
     1141 cacgcgaccg ggcggcttcg gttcaccgac ctcgcgcggt tgtcgaccat cgggctgcgc
     1201 acccgcaaac tgcgagccac cttgtctgca cttggtatcg cgatcggcgt cgcggccgtg
     1261 gtcgccgtcc tcggtttgtc ggcgtcctca caagccgggt tgcttgccga gatcgacaaa
     1321 ctcggcacca acctgctcac cgtgaccaac gggcagacgg tgttcgggca aaacgccgaa
     1381 ctccccgtgg ccgcaccggg catgatctcc cgcatcgggc cggtcacctc ggtgcaagcg
     1441 accggatcgg tcaccgcgtc ggtctaccgc agcccgctca tcccggcgat tcagaccaac
     1501 gcgctgagcg tgctggcgag caccctggac ctgcccgcca ccgtcggcac caccctggcg
     1561 gcgggtagct acctcaacgc cgcgaccgcg accgaacccg tcgcggtact cggatcgtct
     1621 gctgccagcc gcctgggcat cgatcggatc taccccggcg aacgcatctg ggccggcaac
     1681 caatggttct acgtcgtcgg catcttgaac ccggcagtgc ttgcccccga actcgatgcc
     1741 gccatgctgg tgggattccc ggctgcgaaa acctacctgg gattcgacgg gcacccctcg
     1801 acgatctatc tgcgcgcgcg caccgaccaa gtcgccgccg tccaggccgt gctcgccgcc
     1861 acggccgatc cggaagcgcc gaatcaagtc aacgtcagcc aaccttcagc tgccctcgtc
     1921 gctcgagccg aaacgaagag tgctttaaac gggctgttct tggggctcgg cgctgtcgcg
     1981 ttgctggtcg gcgcgatcgg cgtcgcgaac atcatggtga tctcggtatt ggagcgtcgc
     2041 tccgagatcg gccttcgccg cgcgctcggc gccaccaaag gccacatccg cacgcagttc
     2101 ctctctgagg cgatcgtgct ggctgcgatc ggcggcgcgg cgggggtcgg tgccggcgcg
     2161 cttgcgaccg cgatctatgc gagcaccaaa agctgggcaa tcgtcgtccc gcctatcgcc
     2221 tggggaggca gcctcggcgc cgcgctgctc atcggcgccg tcgccggcct gctccccgca
     2281 ctgcgcgccg cccggatgcc cccgacccga gccctgtgga gcatgtaact gggagggccc
     2341 gcttgtcgtc ggtcggtaac cttgagggtg tgaccgactc cgaggctgct aaggc
//
LOCUS       DATCBJ010000500         2497 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_491373, whole genome shotgun sequence.
ACCESSION   DATCBJ010000500 DATCBJ010000000
VERSION     DATCBJ010000500.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2497)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2497)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2497
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_491373"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..195)
                     /locus_tag="VII50_10190"
     CDS             complement(<1..195)
                     /locus_tag="VII50_10190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5051259.1"
                     /translation="MLPDVNPNGPDADQPAPRRPHAILRYTTLRLALFVLALIVLWLV
                     RIRDGVLLVAIALVLSGLASF"
     gene            complement(197..1345)
                     /gene="mqnE"
                     /locus_tag="VII50_10195"
     CDS             complement(197..1345)
                     /gene="mqnE"
                     /locus_tag="VII50_10195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminofutalosine synthase MqnE"
                     /protein_id="HEY5051260.1"
                     /translation="MDTGRKRELEDKVLAGQRLTYDDGVALYASDDLAWLGELAHYVR
                     TQKNGDRTYFNVNRHLNLTNVCAASCAYCSFQRKPGETDAYTMRIEQAVALAKQMEPE
                     GITELHIVNGLHPTLPWRYYPRSLKALKEALPNVALKAFTATEIHWFEEISGLSADEI
                     LDELIDAGLESLTGGGAEIFDWEVRQKIVDHKTHWEDWSRIHRLAHSKGLKTPCTMLY
                     GHIEEPRHRVDHVLRLRELQDETGGFVVFIPLRFQHDASGDPRNRLMTQPMATGAEAL
                     KTFAVSRLLFDNVPHVKAFWVMHGLTTSQLALSHGADDLDGSVVEYKITHDADHFGTP
                     NTMHRDDLLELIRDAGFTPVERDTRYNVIRVFDGPDTGRRDVPQPMPV"
     gene            1683..1904
                     /locus_tag="VII50_10200"
     CDS             1683..1904
                     /locus_tag="VII50_10200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BldC family transcriptional regulator"
                     /protein_id="HEY5051261.1"
                     /translation="MENRDRLLTPGEVASLFRVDPKTVTRWAASGRIGSIRTPGGHRR
                     FRESEVRALLRGDADLDSIGSLALEASEG"
     gene            complement(1966..>2497)
                     /locus_tag="VII50_10205"
     CDS             complement(1966..>2497)
                     /locus_tag="VII50_10205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0006725 - cellular aromatic compound
                     metabolic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HEY5051262.1"
                     /translation="AARLTLLDETGVAWHDDVDSLHAWLTSRLDADLPPTAADGVDRV
                     GFWSPGDLAAGPSSGSYITRGMVVVPASTASVAGIAIGLSKDLLQRAADVTLKEQRPL
                     IVVPREMPLTGATLRHLVTLDTAGAVVLPASPAFYSKPKSMQQLVDFVAGKILDVLGI
                     DHDLFARWDGRLGPES"
BASE COUNT          452 a          785 c          816 g          444 t
ORIGIN      
        1 gaagctcgcg agacccgaga gcacgagtgc gatggcaacc agcagcacgc cgtccctgat
       61 ccgcaccagc cataacacga tgagcgcgag gacgaagagt gccagccgca acgtcgtgta
      121 ccgcaagatc gcatgcggac ggcgcggcgc cggctgatcg gcgtccgggc cgttgggatt
      181 gacgtccggt agcacgtcag accggcatgg gctgcgggac gtcgcgtcgg cccgtgtctg
      241 gtccgtcgaa aacgcgaatc acgttgtagc gcgtgtcgcg ctcgacgggc gtaaagccgg
      301 cgtcgcgaat gagctcgagc aggtcatcgc ggtgcatggt gttcggtgtt ccgaagtggt
      361 cggcatcgtg ggtgatcttg tactcgacca cggagccgtc gagatcgtcg gcaccatgcg
      421 acagcgcgag ctgcgaggtc gtcagcccgt gcatcaccca gaacgccttg acgtgtggca
      481 cgttgtcgaa cagcaagcgc gagactgcga aagtcttcag ggcctcggcg ccggtcgcca
      541 tcggttgggt catcagccgg ttgcgagggt cgccgctcgc atcgtgctgg aaccgcagcg
      601 gaatgaacac cacgaagcca ccggtctcgt cctgcagctc gcgcaggcgc agcacgtggt
      661 cgacccgatg ccgcggctcc tcgatgtggc cgtagagcat cgtgcagggc gtcttcaggc
      721 ccttcgagtg agccaaccgg tggatccgtg accagtcttc ccagtgggtc ttatggtcga
      781 cgatcttctg ccgcacttcc cagtcgaaga tctcagcccc gccgccggtc agcgactcga
      841 gcccggcgtc gatcagttcg tcgaggatct cgtcggcgga cagaccggag atctcctcga
      901 accagtggat ctcggtcgcg gtgaacgcct tcagcgcgac gtttggcaac gcttccttga
      961 gcgctttcaa cgagcgcggg taataccgcc agggcaaagt cgggtgcaag ccgttcacga
     1021 tgtgcagctc ggtgatgccc tcgggctcca tctgcttggc gagcgccaca gcttgttcga
     1081 tgcgcatcgt gtacgcgtcg gtctcgccgg gcttgcgctg gaaactgcag taagcgcacg
     1141 acgcggcgca cacgttggtg aggttcaggt gccggttcac gttgaagtag gtgcggtcgc
     1201 cgttcttctg cgtgcgcacg tagtgcgcga gctcgccgag ccaggccagg tcgtcgctgg
     1261 cgtacaaggc gacgccgtcg tcgtaggtca atcgctgtcc ggcaagaacc ttgtcttcga
     1321 gctcgcgctt gcgtccggtg tccacgcttc aagcatgcac gtccgcgttt gatcgcgctc
     1381 gtgcggggcg cgtgcggggc gggcatgagg caagcaacgg acgatgcgcg cgtcatgacg
     1441 ctcccatatt ccttcgtcac tgacagcaat catgcggcga ctgttagccc ctatagcggc
     1501 cgttgtgccc aaattccgct actaggagtg accgcctcgt aacgaccacc ccgacttttt
     1561 tgtccgcaac acaactaacg tgcgccaaac gcagacgaga cgacacatcc gcgtgtacac
     1621 atctgggagg ggtcccgcga aagcaattcg cagcaccgcg tcccgcagaa aggcacatca
     1681 cagtggagaa ccgtgaccgg ctcctgacac ccggagaggt tgccagcttg tttcgggtcg
     1741 acccgaagac cgtgacacgc tgggcagctt ccggccggat cggcagcatc cgcacgcccg
     1801 gtggccatcg gcgcttccgg gaatccgaag ttcgggctct gttgcgtggc gacgccgatc
     1861 tcgactcgat cggatcgctt gcgttggaag cctcggaggg ctgagcttcg ggcacgagtc
     1921 ctgaggttcg ggggcgggtg gcgcgaacgc gcgtcgcccc taggtctagc tctcggggcc
     1981 taagcgcccg tcccaacgtg cgaacagatc atggtcgata ccgagaacgt cgaggatctt
     2041 gcctgcgacg aagtcgacca actgctgcat gctcttgggc ttgctataga aagccggact
     2101 tgcagggagc accacagcgc ccgccgtatc gagggtgacc agatggcgca gcgtagcgcc
     2161 cgtaagaggc atttcccgcg ggacgacgat cagcggtcgc tgttccttca gtgtcacatc
     2221 tgcggcccgc tgcaagagat ccttcgacaa cccgatcgca atgcccgcga ctgacgcggt
     2281 actggcaggc acgacgacca tgccgcgggt gatgtatgag ccactcgatg ggccggctgc
     2341 gagatcaccg ggtgaccaga agcccacgcg atcgacgccg tccgcagctg taggaggcag
     2401 atcggcgtcc agccgtgacg tcaaccacgc atgaaggctg tcgacgtcgt cgtgccacgc
     2461 gacaccagtc tcatcgagca aagtgagccg cgcggct
//
LOCUS       DATCBJ010000501         3502 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_343909, whole genome shotgun sequence.
ACCESSION   DATCBJ010000501 DATCBJ010000000
VERSION     DATCBJ010000501.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 3502)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3502)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3502
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_343909"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            127..699
                     /locus_tag="VII50_10210"
     CDS             127..699
                     /locus_tag="VII50_10210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5051263.1"
                     /translation="MTHLGDLVCVLIDDELAGDAYGHAVVHLDECAQCRRDVAEQRWV
                     KAQLVQAQPAAVPDPVLLARLLQIPESDDARPDPQSSRQLRLSGPLSGSVRSRRLLGR
                     TAPSRPLRTGQPAGRRAHPRTVAAAGFAASVVVGLSGAISGSAAVSSGAGASATTPSV
                     FTAAALGAPISAVVPRPGARVAVSVVYRRP"
     gene            complement(796..1950)
                     /locus_tag="VII50_10215"
     CDS             complement(796..1950)
                     /locus_tag="VII50_10215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
                     /protein_id="HEY5051264.1"
                     /translation="MADQLADRARPTDESVRAALATVQDPDIRRPITELGMVESILIR
                     DDGRVAVGVLLTVPGCPLKEKITADVTAAVRAVPGVTGVEVTLGFMTDEQRKALQLQL
                     RGPGKPEKEIPFNDPNSLTRVLAIASGKGGVGKSSITVNVAAALAARGLSVGLLDADV
                     YGHSVPRMLGVQGRPTQVAGMIMPPTAHGVRVISIGMFVEGNTPVAWRGTMLHRALNQ
                     FLTDVYWGDLDVLLVDLPPGTGDIAISLAQFVPGSELIVVTTPQQAAAEVAERAGTIA
                     LQTRQRVAGVVENMAWLPCPHCGERVDVFGSGGGQTVVDALSHAFGTQIPLLGQIPLD
                     PGLVTAGDTGVPFVLSHPQAPASVELFRLADALAIRERSLVGRPLGLQPV"
     gene            1984..2451
                     /locus_tag="VII50_10220"
     CDS             1984..2451
                     /locus_tag="VII50_10220"
                     /inference="COORDINATES: protein motif:HMM:NF024212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEY5051265.1"
                     /translation="MVDAAQPAGDRRRAPSAAEIAVWRSFLRAHAQLIRTLEAELAQA
                     ADLPIASYDVLVQLAEAGDHRLRMTDLADRVLLSRSGLTRVIDRLERDGLVSREPVPD
                     DARGMYAVLTRAGVDRLRSASHVHLRGIGAHFVQRFNRRELDRLGQLLGRLVD"
     gene            complement(2424..>3502)
                     /locus_tag="VII50_10225"
     CDS             complement(2424..>3502)
                     /locus_tag="VII50_10225"
                     /inference="COORDINATES: protein motif:HMM:NF024183.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HEY5051266.1"
                     /translation="RRPCADCVAPYSPSARVLTLLGLTESDLVGATPMRGRGCGECGG
                     TGYRGRTGIFEVLPVTASVRSVLSHTPTEAAVGAAARAAGMTTLRGSGLARARRGETT
                     FEEVLRVSQVDMTEGHRCTACDRAVDADMIACPWCATIVDRGHCSSCARPLESGWKIC
                     PWCRTSAVVPEAATDTSVRIPRLLVVGDNGAVHSLIRSAVDDAMDVDVAATADDALTA
                     VWDGEYDGVIVQDTLPDIGGVELLRLLRSGTRTAALPLMLVTEPSDSGSPADDALHSG
                     VDEVLSLDAAPAQTLTQLRLLTERSPHAGAWRAAPSTAGKATETKPVEPTDEIAVPKP
                     NRRVRSVPVLAERRDRQSTSRPKS"
BASE COUNT          535 a         1205 c         1194 g          568 t
ORIGIN      
        1 acatcgtggc cgcacccagt tgcgcgccgc gctcgagcac cgcgccccgg accacagtcg
       61 gcggccgcgg gtcagcacgc cgaggccggc tcggacccca cgcgggatgc cggggctagc
      121 cacgtcgtga cccatctcgg cgacctggtc tgtgtgctca tcgacgatga gctcgctggc
      181 gacgcgtacg gtcacgcggt tgtgcacctg gacgagtgcg cccaatgtcg gcgtgacgtc
      241 gcggagcaac ggtgggtgaa ggcgcagttg gtgcaagcgc aacccgcggc cgtgcccgat
      301 cccgtgttgc tcgcgcggct gctccagata cctgagtccg acgatgcgcg cccggatcca
      361 caatcgtcgc gccagttgcg tttgtcgggc ccgttgtcag gctcggtacg gtcacgacgg
      421 ctgctcggcc gcactgcgcc gtcccgaccg cttcgcaccg gccaacctgc tggtcgccgc
      481 gcgcatccgc ggacggttgc cgccgccggc ttcgccgcga gcgtggtggt cgggttaagc
      541 ggcgcgatct caggcagcgc cgccgtgtca tcgggtgcgg gcgcgtccgc aaccacgccg
      601 tcggtgttca cggccgccgc gttaggcgca cccatctccg cagtagtccc gaggccgggc
      661 gcccgagtag cagtctcagt ggtctaccgc cgcccctaag gcgcgtcacc ctccgcgccg
      721 ccgcactctc cgcggtcccg cggcactcgg cctgaacctg aatgtgtgag gtatgggcgc
      781 gaacgggcgc gcgagttaga cgggctggag tcctagcggg cggccgacca gactgcgttc
      841 gcggatcgcg agcgcgtcgg cgagcctgaa cagctcaacc gacgccgggg cttgcggatg
      901 gctcaggacg aacggcacgc cggtgtctcc ggcagtgacc agccccgggt cgagcgggat
      961 ctggccgagc aacgggatct gggtgccaaa ggcgtgtgac aaggcatcga cgacggtctg
     1021 cccgccgccg gatccgaaga cgtcgacccg ctcgccgcag tgcgggcatg gcaaccaggc
     1081 catgttctcg accacacccg cgacgcgttg ccgagtctgc aaggcgatgg tgccggcgcg
     1141 ctcggcgacc tcagcggcag cctgctgcgg cgtcgtcacg acgatgagct cggaccccgg
     1201 cacgaactgc gccagcgaga tggcgatgtc gccggtgccg ggcggcaggt cgacgagcag
     1261 gacgtcgagg tcgccccaat agacatcggt caggaactga ttgagtgcgc gatgcagcat
     1321 cgtcccgcgc caggcgacgg gagtgttgcc ctcgacgaac atgccgatcg agatgacccg
     1381 gacgccgtgt gctgtcggcg gcatgatcat gccggccact tgagtcggcc ggccttgcac
     1441 tccgagcatc cgcggcaccg aatggccgta cacgtccgcg tcgaggaggc caacggaaag
     1501 cccgcgcgcg gcgagcgccg ccgcaacgtt cacggtgatc gacgacttgc cgacgccacc
     1561 cttgcctgag gcgatcgcca gaacgcgcgt cagcgagttc gggtcgttga agggaatctc
     1621 tttctccggc ttacccggcc cccgcagttg cagttggagt gctttgcgct gctcgtccgt
     1681 catgaatccg agggtgacct cgacgccggt gacaccgggc actgcacgca ccgccgcggt
     1741 cacgtcagcg gtgattttct ccttgagcgg gcagcccggc accgtaagca gcacgccgac
     1801 cgccacccgg ccgtcgtcac gaatgaggat cgactcgacc atgccgagtt cggtgatcgg
     1861 ccggcgaatg tcgggatcct ggacggtcgc aagcgcggcc cggacggatt cgtcggtcgg
     1921 tcgagcccgg tctgccagtt ggtctgccac tcaccaatgc tactcggacc tacgatgcct
     1981 tccgtggtcg acgcagcgca gcccgcaggg gaccgtcgtc gtgccccttc ggcggcggag
     2041 atcgcggttt ggcgatcgtt cttgcgcgcg cacgcccagt tgattcgcac actcgaagcc
     2101 gaactggcgc aggccgctga cctgccgatc gcctcgtacg acgtgcttgt gcagctggcc
     2161 gaggcaggtg atcatcggct gcgcatgact gacctcgccg atcgggtcct cctgtcgcgc
     2221 agcggcctca cccgagtgat cgacaggctc gagcgcgacg gactcgtcag ccgcgagccg
     2281 gtccctgacg acgcgcgtgg catgtacgcc gtcctgaccc gggccggcgt cgaccggttg
     2341 cggtcggcgt cgcacgtgca cctacgaggg ataggcgccc acttcgtgca gcgcttcaat
     2401 cgccgcgaac tcgatcggct cggtcagctc ttggggcggc tggtcgactg acgatctcgg
     2461 cgctcggcga gcaccgggac agaacgcact cgacgattcg gctttggaac cgcgatctcg
     2521 tcggttggct ctaccggttt ggtttcagtg gctttcccag cggtggacgg cgccgcgcgc
     2581 caggccccag cgtgcgggct gcgctcggtc agcaatcgca gctgcgtcaa tgtctgcgcc
     2641 ggcgctgcat ccagcgacaa cacctcatcg actccactgt gaagcgcatc gtctgcgggt
     2701 gatccgctgt cactgggttc cgtgacgagc atcaacggca gcgcggcggt tctggtcccg
     2761 ctgcgcaaca gccggaggag ttcgacgccg ccgatgtcgg gcaaggtgtc ttgcacgatc
     2821 acgccgtcgt attcgccgtc ccacactgca gtgagggcgt cgtcggcggt agccgcgaca
     2881 tcgacgtcca ttgcatcgtc gaccgcagac ctgatcaggc tgtgcactgc gccgttgtcg
     2941 ccgacaacga gcaaccgcgg tatccggacg ctggtgtctg tggcggcttc cgggacaact
     3001 gcggatgtac ggcaccacgg gcagatcttc caccccgact cgaggggccg cgcacacgaa
     3061 ctgcagtggc cgcggtcgac gatcgtcgcg caccaagggc acgcaatcat atcggcgtcg
     3121 acggcacgat cacacgcggt gcaccggtgt ccctcggtca tgtcgacctg ggaaacgcga
     3181 agcacttctt cgaacgtcgt ctctccgcgt cgcgcgcgag ccagacctga tccgcgcagt
     3241 gtcgtcatcc cagctgcgcg tgcggcggcg ccgacggctg cttcggtggg cgtgtgcgag
     3301 agcacggatc gcacgctggc tgttacgggc aggacttcga agattccagt tctgccgcga
     3361 tacccggttc cgccgcattc gccgcagccg cggccgcgca tcggtgtcgc gccgacaagg
     3421 tcggattcgg tgagcccgag caaagtgagc acccgggcac tcgggctgta cggcgccacg
     3481 cagtcagcgc acggacgccg ga
//
LOCUS       DATCBJ010000502         3265 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_491456, whole genome shotgun sequence.
ACCESSION   DATCBJ010000502 DATCBJ010000000
VERSION     DATCBJ010000502.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 3265)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3265)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3265
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_491456"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            426..800
                     /gene="rpsL"
                     /locus_tag="VII50_10230"
     CDS             426..800
                     /gene="rpsL"
                     /locus_tag="VII50_10230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007078642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HEY5051267.1"
                     /translation="MPTIQQLVRKGRQDKVDKTKTPALKGSPQRRGVCTRVYTTTPKK
                     PNSALRKVARVRLTSGIEVTAYIPGVGHNLQEHSIVLVRGGRVKDLPGVRYKIIRGSL
                     DTQGVRNRKQARSRYGAKKEKS"
     gene            801..1271
                     /gene="rpsG"
                     /locus_tag="VII50_10235"
     CDS             801..1271
                     /gene="rpsG"
                     /locus_tag="VII50_10235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006245075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HEY5051268.1"
                     /translation="MPRKGPAPKRPIIIDPVYASPLVTSLVNKVLTNGKRSLAERIVY
                     GALEGCRTKTGTDPVITLKRALDNVRPTLEVKSRRVGGATYQVPMEVKGTRGGTLALR
                     WIVGYSKARREKTMTERLMNELLDASNGLGASVKRREDTHKMAESNKAFAHYRW"
     gene            1343..>3265
                     /gene="fusA"
                     /locus_tag="VII50_10240"
     CDS             1343..>3265
                     /gene="fusA"
                     /locus_tag="VII50_10240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HEY5051269.1"
                     /translation="MADLAKVRNIGIMAHIDAGKTTTTERILFYTGINYKIGEVHEGA
                     ATMDWMEQEQERGITITSAATTCMWNDHTINIIDTPGHVDFTVEVERSLRVLDGAVAV
                     FDAVAGVEPQSETVWRQADKYHVPRICFVNKMDRVGAEFHRTVDMMITRLNAIPLVIQ
                     LPWGVEGDFKGVIDLVKMKGIEWTGEGKGDTYNVVDIPADHAEAARDWHDKLIETLSE
                     QDDELMQQYLDGVEPTVEQTQAGIRRATIASKLNPVLCGSAFKNKGVQPMLDAITDYL
                     PSPLDIGDTIGHKLGDEEVEVRRRPSDTEPFSALAFKIMSDKYLGKLTYLRIYSGTLT
                     TGAALLNSTKDRKERIGRLLRMHANKQEDIPSAGAGSIVAVAGLKNTTTGDTLCDPDK
                     PVILERMIFPTPVISVAIEPKTKVDQEKLGVAIQRLAEEDPTFQVHTDEETGQTIIAG
                     MGELHLDILVDRMKREFNVEANVGKPQVAYRETIRRKVEKVDYTHKKQTGGSGQFARV
                     VIDIEPGGGEGDSGYEFVNKVSGGRIPREYIPAVDAGCQEALQFGILAGYPLVDIKVT
                     LQDGAFHDVDSSELAFKIAGSMAFKEAARKADPTLLEPIMAVEVRTPEDYMGDVIGDI
                     NSRRGIIQSMDELSGTR"
BASE COUNT          682 a         1064 c         1037 g          482 t
ORIGIN      
        1 cgagcgcgac attctggcgt cgggtggagc caagcaaggc ctcggcatgg tcacgggagc
       61 ccggatcacg gcctgggcga acctcgtccc tgtgaccctg ttcacgatcg tcgtgatcat
      121 tggagtggtg gcggcccggc acagcggggg ctacacgggg cactagctcg cggcggtgtg
      181 ggtgggaccc ggttttgacc ttcgccgatg cgatgggtaa tctgactcct cgtgcccgga
      241 accacgggcc gctcgttgtg ctgtccgtgc gtgccgtccg agtgtgtgac gtcgggtatg
      301 ggtacgacag cacaatcacc ctcgatccga gttttcggcg gatcggagcg tgggcgggcg
      361 acacacccga ccgcgtgggt cggcgatagc ggtacgaaac ggtataaaga gactgaaagg
      421 cgcgcatgcc cacgatccaa cagctggtcc gcaagggccg gcaggacaag gtcgacaaga
      481 ccaagacccc ggcgctcaag ggttcccccc agcgccgcgg cgtctgcacg cgcgtctaca
      541 cgaccactcc caagaagccg aactcggcgc tgcgcaaggt ggcccgcgtc cggctgacca
      601 gcggcatcga ggtcacggcc tacattcccg gcgtcggcca caatctgcag gagcactcga
      661 tcgtgctcgt gcgtggtggc cgggtgaagg acctgcccgg cgtccggtac aagatcatcc
      721 gcggctcgct cgacacgcag ggcgtccgca accgcaagca ggcgcgtagt cgctacggcg
      781 cgaagaagga gaagagctag atgccccgca agggtccagc ccccaagcgc cccatcatca
      841 tcgacccggt ctacgcgtcg cccctggtca cctcactggt gaacaaggtg ctgaccaacg
      901 gcaagcggtc actcgccgag cgcatcgtct acggcgccct cgagggctgc cgcaccaaga
      961 ccggcaccga cccggtgatc acgctgaagc gcgctctcga caacgtgcgc cccacgctcg
     1021 aggtcaagag ccgtcgcgtc ggtggcgcta cctaccaggt cccgatggaa gtcaagggca
     1081 cccgcggtgg gacccttgcc cttcgctgga tcgtcggcta ctccaaggcc cgccgcgaga
     1141 agacgatgac cgagcgcctc atgaacgagc tcctcgacgc aagcaacggc ctgggcgcga
     1201 gcgtcaagcg gcgcgaggac acccacaaga tggccgaatc gaacaaggcg ttcgcgcact
     1261 accgctggta atcgaagaac cgaacagcgc aagacccgag gaacagagaa ggaagtagct
     1321 cacagaagtg gcaactgacg tcatggcaga tctagccaag gtccggaaca tcgggatcat
     1381 ggctcacatc gacgcgggca aaaccacgac gaccgagcgg atccttttct acacgggcat
     1441 caactacaag atcggcgaag tccacgaggg cgcagccacg atggactgga tggagcagga
     1501 gcaggagcgc ggcatcacca tcacgtccgc ggcgacgacc tgcatgtgga acgaccacac
     1561 catcaacatc atcgacacgc ccggtcacgt cgacttcacc gtcgaggtag agcgttcgct
     1621 tcgcgtgctg gacggcgcgg tggcggtgtt cgacgcagta gctggcgtcg agcctcagtc
     1681 cgaaacagtc tggcggcagg ccgacaagta ccacgtaccc cgcatctgct tcgtcaacaa
     1741 gatggaccgt gttggcgccg agttccatcg caccgtcgac atgatgatca cccggctgaa
     1801 cgcaatcccg ctggtcatcc agctgccgtg gggtgtcgaa ggcgacttca agggcgtcat
     1861 cgacctggtg aagatgaagg gcatcgagtg gaccggcgag ggcaagggcg acacctacaa
     1921 cgtcgtcgat atccccgccg accatgctga ggccgctcgc gattggcacg acaagctcat
     1981 cgagacgctg tccgagcagg acgacgagtt gatgcagcag tacctcgacg gtgtcgagcc
     2041 gacggtcgag cagacccagg cgggcatccg ccgggccacg atcgccagca agttgaaccc
     2101 ggtgctgtgc ggcagcgcgt tcaagaacaa gggcgtgcag cccatgctcg acgcaatcac
     2161 cgactacctg ccgagcccgc tcgacatcgg cgacaccatc ggtcacaagc tgggcgacga
     2221 agaggtcgag gtgcgacgca ggccgagcga caccgagccc ttctcggcgc ttgcgttcaa
     2281 gatcatgagc gacaagtacc tcggcaagct cacgtacctg cgcatctact caggcacgct
     2341 gacgaccggt gcagccctgc tgaactccac caaggaccgc aaagagcgca tcggccgtct
     2401 ccttcgaatg cacgcgaaca agcaggaaga catcccgtcg gcgggcgccg ggtcgatcgt
     2461 cgcggttgcg ggtctgaaga acaccacgac cggcgacacg ttgtgcgacc cagacaagcc
     2521 ggtgattctt gagcgcatga tcttcccgac accggtcatc tcggtggcca tcgagccgaa
     2581 gaccaaggtc gaccaggaga agctcggggt cgccattcag cgtcttgctg aagaggatcc
     2641 gaccttccag gtgcacaccg acgaagagac cggccagacg atcatcgccg ggatgggcga
     2701 gctacacctc gacatccttg tcgaccgcat gaagcgcgaa ttcaacgtcg aagccaacgt
     2761 tggcaagccg caggtcgcct accgcgagac catccgccgc aaggtcgaga aggtcgacta
     2821 cacgcacaag aagcagaccg gcggttccgg ccagttcgcg cgcgtcgtca tcgatatcga
     2881 gcccggtggc ggcgagggcg actcgggcta cgagttcgtg aacaaggtca gcggtggtcg
     2941 catcccgcgc gagtacattc ccgcggtcga tgccggctgc caagaagcgc tgcagttcgg
     3001 catcctggcc ggctacccgc tcgtcgacat caaggtcacc ttgcaggacg gcgcgttcca
     3061 cgacgtcgac tcctcggaac tggccttcaa gatcgccggc tcgatggcgt tcaaggaggc
     3121 ggcccgcaag gccgacccca cgctgctcga gcccatcatg gccgtcgagg tacgcacgcc
     3181 cgaggactac atgggcgacg tcatcggcga catcaacagc cgtcgcggca tcatccagtc
     3241 gatggatgag ctgtccggca cccga
//
LOCUS       DATCBJ010000503         2559 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_431581, whole genome shotgun sequence.
ACCESSION   DATCBJ010000503 DATCBJ010000000
VERSION     DATCBJ010000503.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2559)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2559)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2559
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_431581"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..329
                     /locus_tag="VII50_10245"
     CDS             <1..329
                     /locus_tag="VII50_10245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009078063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TetR family transcriptional regulator C-terminal
                     domain-containing protein"
                     /protein_id="HEY5051270.1"
                     /translation="LVQLMRDLRNRDAGWLGVQLELSRRLRTDPDFRGRWAARSDTIG
                     RAARERLQRGVERGTLRTDVPVDTLAAFLELVLDGLVLHRAMGVPDDDLDAVLDLVES
                     AVRRPA"
     gene            complement(343..2277)
                     /locus_tag="VII50_10250"
     CDS             complement(343..2277)
                     /locus_tag="VII50_10250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY5051271.1"
                     /translation="MGQGLSTWHAMRSFTQDSSVTKRKLAPGTVKRIIKFAAPYKRLV
                     ALLLVLLALDAALGAVTPLLIREILNKGIGNKGVGSHPHLVIMLALLIGLIAVVDAGL
                     GLVERYQTSRIGEGLIFDMRARIFSHIQQMPIAFFTRTQTGALISRLNNDVLGAQQAF
                     TSTLSSVVTNVLTVTFTLSVMFVLSWQITLAALALLPLFVLPARWFGRKLQTITRESY
                     NLNAAMNNTMTERFNVAGALLVKLFGRPREEDSAFRAKAGRVRDIGVTQSMYGGVFFA
                     SLMLVASLGVALVYGWGGWLAAKRELDVGTVVALGAYLTRLYGPLTALSNVNVSIMTA
                     LVSFERVFEVLDLPPMIDDAPDAVDLPAGANTVEFDHVEFGYPAASDVSLASLESVAV
                     LDTSSNPQVLHDVSFRADAGQLVALVGPSGAGKTTISALVSRLYDVTGGAVLIGGVDV
                     RQVTQSSLRDTVGVVTQDSHMFHDTIRANLVYAKPDATEDELVEACKAAQIWDLVASL
                     NDGLDTLVGERGYRLSGGEKQRLAIARLLLKAPAIVVLDEATAHLDSESEAAVQRALK
                     VALSGRTSLVIAHRLSTIRDADQILVIDDGRVVERGHHDDLFASGGLYAELYRTQFER
                     QATGELGDLDLADVAPEPLG"
BASE COUNT          460 a          852 c          829 g          418 t
ORIGIN      
        1 gcttggtcca gctcatgcgt gacctgcgca accgcgacgc gggctggctc ggcgtccagc
       61 tggagttgtc gcgccgcctg cgtaccgacc ccgactttcg cggccgctgg gcggcgcggt
      121 cggacaccat cggccgtgct gcgcgcgagc gtcttcagcg cggggtcgag cgtgggactc
      181 ttcgcaccga cgtgccggtc gacactctcg cggcattctt ggagctcgta ctggacggac
      241 tcgtcctgca tcgagcgatg ggagtgcccg acgacgacct cgacgcggtg cttgatctgg
      301 tcgagagtgc cgtgcggcga cccgcatagc gcaggcggcg actcagccca acggttcggg
      361 agccacgtcg gcaagatcga gatcgccgag ctcgccagtg gcctgccgtt cgaattgggt
      421 gcgatagagc tccgcgtaga gaccgcccga ggcaaacaag tcgtcgtggt ggccgcgctc
      481 aacgacgcga ccgtcgtcga tcaccaagat ctggtcggcg tcgcggatcg tggacaaccg
      541 gtgggcaatc acgagcgacg tccggcccga aagggcgacc ttcagagccc gctgcacagc
      601 tgcctcggac tcggagtcga ggtgcgcggt cgcctcgtca agcaccacga tcgccggcgc
      661 cttgagcaac agccgggcga tcgcgagccg ctgcttttcg cccccggaca accggtagcc
      721 gcgctcgccg accaaagtgt cgaggccgtc gttcaacgac gccacgagat cccagatctg
      781 tgccgccttg cacgcctcga cgagctcgtc ctcggtggcg tcgggctttg cgtagaccaa
      841 gttggcccgg atcgtgtcgt ggaacatgtg actgtcctgg gtcaccacac cgaccgtgtc
      901 gcgcagcgac gactgggtca cttgccgtac gtcaacgccg ccgatcagca cggctccccc
      961 ggtgacgtcg tacagccgtg acaccagcgc ggagatcgtc gtcttgcctg cgcccgacgg
     1021 gcctaccagc gccacgagct ggccagcgtc ggccctgaac gaaacgtcgt gcagcacctg
     1081 cggattcgac gacgtgtcga gcacggccac cgattcgagc gaggccaatg acacgtcgga
     1141 cgccgccggg tagccgaact cgacatggtc gaactcgacg gtgttggcac cagccggcag
     1201 gtcgaccgcg tcgggcgcgt catcgatcat cggcggtagg tcgagaacct cgaacacccg
     1261 ctcgaacgac acgagcgccg tcatgatgga gacgttcacg ttcgacaaag ccgtgagtgg
     1321 gccatacagc ctggtcaggt acgcgccgag cgcaacgact gtcccgacgt cgagttcgcg
     1381 cttcgcagcg agccaaccgc cccatccgta gacgagcgcg acgccgagcg atgcaaccaa
     1441 catcaatgag gcgaagaaga cgccgccgta catcgactgg gtgacgccga tgtctcgaac
     1501 ccggccggcc ttcgcgcgaa atgcggagtc ttcctcacgc gggcggccga agagcttgac
     1561 cagcagggct ccggccacgt tgaagcgttc ggtcatggtg ttgttcatgg cggcgttgag
     1621 gttgtagctc tcgcgggtaa tcgtctgcag cttgcggccg aaccagcgag ccggcagcac
     1681 aaacagcggc aacaacgcaa gcgcggcgag cgtgatctgc cacgagagta cgaacatcac
     1741 cgagagcgtg aaagtcacgg tgagaacgtt ggtcacgacg ctggacaacg tgctcgtgaa
     1801 cgcctgttgc gcaccgagca cgtcgttgtt caaacggctg attagggcgc cggtctgtgt
     1861 gcgggtgaag aacgcgatcg gcatctgttg aatgtggctg aaaatccttg cccgcatgtc
     1921 gaagatgagg ccctcgccga tgcgcgacgt ctggtaccgc tccaccaacc ccaggccggc
     1981 atcgacgacc gcgatcaacc cgataagaag cgcgagcatg atgacgagat gggggtgcga
     2041 gccgacgccc ttgtttccga tgcccttgtt caggatctcg cgaatcagca acggggtcac
     2101 cgcgccgagc gctgcatcga gcgcgagcag caccagcaac agcgcgacca gccgcttgta
     2161 cggagcggcg aacttgatga tccgcttgac cgtgcctggt gccagtttcc gcttggtcac
     2221 cgaggagtcc tgggtgaagc tgcgcatcgc atgccaggtg ctcaggcctt ggcccatgct
     2281 cactgcaaca tcacccgctc atcgtctccc gccgtcgtgc ccccgcagtt agtgccagtg
     2341 tcccaccgct cccaacgcgc cgccgccgct acgacttccc gacggcgtct cggagttagg
     2401 attcggtcgc caggcgtaat cccgcgacga ggtcgacggc ttggcggcgc cgatcggcag
     2461 ccgactcgcg gagtaccgcc ttggcaacga tcgcttcctc ccgtggagtc gcaaggacgg
     2521 ccgcgaccat ctccgcaacc gccgcgtcga gcttctcgg
//
LOCUS       DATCBJ010000504         4082 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_380667, whole genome shotgun sequence.
ACCESSION   DATCBJ010000504 DATCBJ010000000
VERSION     DATCBJ010000504.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 4082)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4082)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4082
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_380667"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1079
                     /locus_tag="VII50_10255"
     CDS             <1..1079
                     /locus_tag="VII50_10255"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY5051272.1"
                     /translation="LPVAGICADRYDVRRLSISALAAQAVCLALLATVMQRGLVALTV
                     LYACQGALSAFWPPVRQQWLYGLVAPELRQQANSAIGSINGVMTLVGATFGGLLSAWH
                     PAAAVFAAAALQLVTVAQLAFVPRSQTASAARVGAARRPRVLGDLVDAFRAARALPLA
                     RSIVWIGIAWGLIGGGYNVLLAGHITRDLHGGAITLGLVYAADGLSVIVATTFAAGLS
                     QRRQLAAYASAYVIQGVAWAMTFAANSLALALMALVVMRLASGYVIALDTTILLQTVP
                     PRLRGRIVSLHMTTYSAVARISLAAVGAALTALSISTIGIATGVASAAFGVVWWWKSG
                     RPARTVYLAPDAAIDPDDESLETR"
     gene            complement(1101..1871)
                     /locus_tag="VII50_10260"
     CDS             complement(1101..1871)
                     /locus_tag="VII50_10260"
                     /inference="COORDINATES: protein motif:HMM:NF016533.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA];
                     GO_process: GO:0019748 - secondary metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase family
                     protein"
                     /protein_id="HEY5051273.1"
                     /translation="MTDTSDANVFDLSKAKSLAIESAAHWDLALEAPFAMSTVSYVAP
                     AGPLVVKVPWGGDDESLHEADALRLWGGNGAVRLLDQLGSVILEERAVPGDDLAAVPD
                     AEATRIAVDVARQIWVAGRTPFRPVVPEVRRWLHEADVAGGDLVPLARDLLKTLGDPV
                     GWLVHGDFHHHNLVRHGERYVAIDPKPYLCEREFDVPTFLWNPRDNVLDDREQIEGRI
                     AAFVGAGLSEWRIRAWTVVRGAYLRPGLASRIRALLDP"
     gene            1939..3150
                     /gene="serA"
                     /locus_tag="VII50_10265"
     CDS             1939..3150
                     /gene="serA"
                     /locus_tag="VII50_10265"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: protein motif:HMM:NF008759.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HEY5051274.1"
                     /translation="MSNATIRALLLEQIHPDADGLLREAGYTVESGGRALGEDELIGA
                     LPGFQLLGIRSKTQVTARVLEAATDLQAIGAFCIGTNQIDLAAAARRGVAVFNAPFSN
                     TRSVVELALAEIIALTRHLTDKNTLMHAGVWDKSAEGAHEVRGRTLGIVGYGNIGTQL
                     SVLAENLGMNVMYYDVIDRLALSNARRCESLYELLAQADVVSLHVDGRHDNANLFGAT
                     EFDAMKAGSLFLNLSRGFVVDYASLRLHIESGHIAGAAIDVFPDEPSGRGDEFVSEVR
                     GLPNVILTPHIAGSTEEAQQDIARFVATKFRDFIAEGSTAMSVNMPRITLRQNPGTHR
                     MILLHRNVPGVLARVNGIFAAHDANVEAQLLATTGEIGYVVTDIGSAFTSEMVAQLGA
                     LPETVRFRLLP"
     gene            3194..>4082
                     /locus_tag="VII50_10270"
     CDS             3194..>4082
                     /locus_tag="VII50_10270"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxide hydrolase"
                     /protein_id="HEY5051275.1"
                     /translation="MPDAPPRPEPFTLHTDPAALDDLRARLRATRWPDAPQDAGWSLG
                     TDLAYLRELVTYWADEFDWPAQEAALARLPRFRVKLGELGIHFVHARAVAPTAPALPL
                     VLSHGWPDSFWRYSKVIPLLTDPGAHGADPADAFDVVVPDVPGYGYSDAPVGPPIDSI
                     AVAGLWAELMTALGYETFCAGGGDIGSHVSRYLALDYPDRVVAVHRTDAGVPLFTGDP
                     ADLTPEEREWFASIAAWGAAEGAYGAMHRTKPQTAAVGLNDSPAGLASWIVEKLQGWG
                     GGDIERSFTKDEILTNVTIY"
BASE COUNT          660 a         1373 c         1307 g          742 t
ORIGIN      
        1 tgttgccggt agcgggtatc tgcgccgacc gctacgacgt gcgtcggctg tccatctccg
       61 cgctggctgc gcaggcggtc tgcttggcgc tgctcgccac ggtgatgcag cgcggcctcg
      121 tcgcgctgac ggtcctgtac gcatgccagg gtgcactgag cgctttctgg ccgcccgtcc
      181 gacagcagtg gctgtacggc ctggtcgcgc ctgaactgcg ccagcaagcg aactcggcta
      241 tcgggtcaat taacggcgtg atgactctgg tgggggcgac cttcggcggc ctgctgtctg
      301 cctggcatcc ggctgccgcc gtcttcgcag cagcggcgct ccaactcgtc actgtggccc
      361 agctcgcgtt cgtaccgcga tcccagacag ctagcgcggc aagggttggc gccgccaggc
      421 ggccgcgtgt gcttggcgac ctggtggatg cgttccgcgc ggcgagggcc cttccactcg
      481 cgcgttccat tgtctggatc gggatcgcct gggggctgat cggtggtgga tacaacgtcc
      541 tattggctgg ccacataacc agggacctac atggcggcgc catcacactt ggcttggtct
      601 acgcagccga cggcttgagt gtcattgtgg ccaccacgtt cgcagccggc ctgtcacaaa
      661 ggcggcagct ggccgcctac gcgagcgcgt acgtcatcca gggagtggcc tgggcgatga
      721 ccttcgcggc caactcgctc gcgctcgccc taatggcact agtagttatg cggctggcca
      781 gcgggtacgt catcgcgttg gatacaacga tcctgcttca gacggtaccg ccgcgcctgc
      841 gtggccgaat cgtgagcctg cacatgacga cgtatagcgc tgtggcgcgc atttcgctcg
      901 ccgccgtcgg cgcagcgctg accgctctca gcatcagcac aatcgggatt gccacgggtg
      961 tcgcctcagc agcgttcggt gtcgtgtggt ggtggaagtc cgggcgcccg gcaagaactg
     1021 tctacctggc acctgacgct gctatcgacc ccgacgatga atcgttggag acacgatgac
     1081 ccgcggtgcc gtcgaccgcg tcatgggtca aggagtgccc tgatgcgcga cgcaagtccg
     1141 gggcgtaggt acgcgccgcg tacgacagtc cacgcgcgga tgcgccactc gctcaggccc
     1201 gcgccgacaa acgctgctat ccgaccctct atctgttccc tgtcgtccaa cacgttgtcc
     1261 ctggggttcc acaggaacgt tggtacgtcg aattcgcgtt cacacaggta cggcttcggg
     1321 tcgatggcga cgtatcgctc gccgtgccgg acgaggttgt gatggtggaa gtctccgtgc
     1381 acgagccagc cgacgggatc accaagggtc tttaacaggt cacgcgctag cgggacgagg
     1441 tcacctccag ctacgtcagc ttcgtgcagc catctccgga cctccgggac gaccggtcgg
     1501 aatggggttc ggcccgccac ccaaatctgc cgagcgacgt cgaccgcgat gcgggtcgcc
     1561 tctgcgtccg gcacggcggc aaggtcgtcg cctgggacgg cacgttcttc gaggatcacg
     1621 gaacctaact gatccagcag ccgaaccgca ccgttcccgc cccacagccg cagagcgtcg
     1681 gcttcgtgca gcgattcatc gtcgcctccc catggcactt tcaccacgag aggccctgcc
     1741 ggtgcaacgt acgacacggt ggacatggcg aagggtgcct ccagtgcgag atcccagtgc
     1801 gccgccgact cgatcgccag cgatttcgcc ttcgacaggt cgaagacatt cgcatcggag
     1861 gtgtccgtca cgacccgatg gtagggcttg tcagggcttg ctccgatcgg ctgatggcct
     1921 acgcgagata ctaagcacgt gtccaacgcc acgatccgcg ccctgttgct tgaacagatc
     1981 catcccgatg ccgacgggct cttgcgcgag gccggctaca cggtcgagtc ggggggccgc
     2041 gcattgggag aggacgagct catcggcgcg ctacccggct tccaactgct tggcattcgc
     2101 tcgaagacgc aggtgaccgc ccgcgttctc gaggccgcaa ccgatcttca ggcgatcggc
     2161 gcgttctgca ttggcaccaa ccagatcgat ctggcggcgg ctgctcgacg tggcgttgcg
     2221 gtgttcaacg ccccgttctc caatacccga agcgttgtcg aactggcgct ggccgagatc
     2281 atcgcgctga ctcggcatct gacggacaag aacacgttga tgcacgcagg ggtttgggac
     2341 aagtccgccg agggcgcgca cgaggttcgc ggccgcacgc tcggcatcgt cggatacggc
     2401 aacatcggca cgcagttgtc agtgctcgcc gagaacctcg gtatgaacgt catgtactac
     2461 gacgtcatcg accggctcgc gctgtcgaac gcgcggcgct gcgagtcgtt gtacgagctg
     2521 ctggcacaag cagacgtcgt ctcgttgcac gttgatggcc ggcacgacaa cgcgaatctc
     2581 ttcggcgcta ccgagttcga tgccatgaag gccggctcgc tgtttctgaa cctctcgcgt
     2641 ggcttcgtcg tcgactatgc atcgctgcgg ttgcacatcg agtcgggcca catcgcaggc
     2701 gccgccatcg acgtgtttcc cgacgagccc agtgggcgag gcgacgagtt cgtgtccgag
     2761 gtcagagggc tacccaacgt catcctgact ccgcacatcg ccggttcgac cgaggaagct
     2821 cagcaggaca tcgcaagatt cgtcgcgacc aagtttcgcg acttcatcgc cgagggctcg
     2881 acagccatga gcgtcaacat gccacgcatc acgctgcggc agaatcccgg cacccatcgc
     2941 atgatcctgc tacaccgcaa cgttcccggc gtgctcgcca gggtcaacgg gatcttcgcg
     3001 gcgcacgacg cgaacgtcga ggctcagctg ctggctacaa ccggtgagat cggctacgtc
     3061 gtgaccgaca tcgggtcggc cttcacatcc gagatggtcg cgcagctagg cgctcttccc
     3121 gagacggtcc ggttccgctt gctgccctga tatccgcgcg accgcgcccg tcgccatcgg
     3181 taatgtgcga cccatgccag acgcgccgcc ccgccccgaa ccgttcacct tgcacaccga
     3241 tcccgcggca cttgacgacc tccgcgcgcg tcttcgcgcg acgcgttggc cggacgcacc
     3301 gcaggacgcc gggtggtcgc tcggcaccga ccttgcgtat ctccgcgaac tcgtcacgta
     3361 ttgggcggac gagttcgatt ggcccgcgca ggaggcggcg ctcgcgcggc ttccgcgctt
     3421 tcgcgtcaag ctcggcgagc tcggcatcca cttcgtgcac gcgcgcgccg tcgccccaac
     3481 ggcgcccgcg ctgcctctgg ttctcagcca cggctggccg gactccttct ggcgctactc
     3541 gaaggtcata ccgctactta ccgatccggg tgcgcatggc gccgatcccg ccgacgcgtt
     3601 cgacgtggtc gtgcccgacg tgccgggcta tgggtactcc gacgcgcccg ttgggccgcc
     3661 catcgattcg atcgcggtcg ctggattgtg ggccgaactt atgaccgcgc tcggctacga
     3721 gaccttctgc gcgggcggcg gcgacattgg cagccacgtc agtcgctacc tcgcacttga
     3781 ctaccccgat cgagtcgtgg ccgtccaccg caccgacgcg ggcgtgccgc tcttcaccgg
     3841 cgacccagcc gacctcacgc ccgaggagcg cgagtggttc gccagcatcg ccgcatgggg
     3901 agcagcagaa ggtgcgtatg gcgcgatgca tcgcacgaag ccacagactg cggcggtcgg
     3961 gctcaacgat tcaccggccg ggctggcgtc ctggatcgtc gagaagcttc aggggtgggg
     4021 cggcggcgac atcgagcgga gctttacgaa ggacgagatc ctcacgaacg tcacgatcta
     4081 ct
//
LOCUS       DATCBJ010000505         3493 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_144815, whole genome shotgun sequence.
ACCESSION   DATCBJ010000505 DATCBJ010000000
VERSION     DATCBJ010000505.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 3493)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3493)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3493
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_144815"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..364
                     /locus_tag="VII50_10275"
     CDS             <1..364
                     /locus_tag="VII50_10275"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HEY5051276.1"
                     /translation="LGAAAVEQEVAAGTVIIREGDEADALWILVSGTLAIQALGPDGS
                     TVELPDVDAPSYVGELGLLHGRPRSATVTVKQDATLLRIDGKEFLNALEAAPPSVSLK
                     TLAGVRLARTPARDDVQD"
     gene            366..1244
                     /locus_tag="VII50_10280"
     CDS             366..1244
                     /locus_tag="VII50_10280"
                     /inference="COORDINATES: protein motif:HMM:NF024118.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEY5051277.1"
                     /translation="MRAFLCGVRGSTPAPGPDFIRYGGHTSSVALAHDGELPSLVIDA
                     GTGLRRVTNLLDGAPFQGSIVLSHLHWDHVHGLPFFQGGARHGHRVNVFLPAIDGDAV
                     GTLSRGLSPPHFPIGPTQLGTGWTFDPLEEGPRQLEGFDVDVREIPHKGGRTFGFRVT
                     DGAGSLAYLGDHFPLGEGAGPDGLGAYHPAALALARDVDVLLHDAQFLAAQFPGVTYL
                     GHSAVEYAIGLATRAGAKALMLFHHAPDRTDDELDAIVQLFADAPVRVIAATEGTVAA
                     LGIPGSPVVTALSTAK"
     gene            complement(1248..2342)
                     /locus_tag="VII50_10285"
     CDS             complement(1248..2342)
                     /locus_tag="VII50_10285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEY5051278.1"
                     /translation="MWATTSALRVRATVLPDADVGEFFVVGGRITFDRPADCDTFADH
                     GFVLPGLVDAHCHVGLAAEGAVPLEEARAHAVTDRDAGALLLRDAGVPHTYEELDTEP
                     DLPRIVHAGRHLARNRRYIRGYGIELEPEQLPDAAAAQARRSGGHGTPWVKLVGDWID
                     RDLGDLAPCWPADVLAEAVRRAHDAGARVAVHVFGEEALPDLIAAGVDSIEHGTGLDD
                     KALGDVVARGIAVVPTLVNVANYDKFADQATKYPLYAARMRRLRRTADGRIAAAHEAG
                     VPIYVGTDAGGQLPHGLVVDEMLALRAAGLSNADVLAAGSWKARRWLGLPGLAEGAPA
                     DFVVYDDDPRADLTVLRSPRHIVVRGTVVR"
     gene            complement(2360..>3493)
                     /locus_tag="VII50_10290"
     CDS             complement(2360..>3493)
                     /locus_tag="VII50_10290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009074441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HEY5051279.1"
                     /translation="LQVLGKQAGVPVFAPEPGSGVGDPVAVSKAAIEHAKRLGHDIVL
                     VDTAGRLGVDAEMMQQAIDIRDAVDPNEILFVVDAMLGQDAVNTAQAFLDGVGFDGVV
                     LTKLDGDARGGAALSVASLTGRQIMFASTGEKLEDFDTFHPERMASRILGMGDILTLI
                     EQTQSAYDAGEAEETARKLTEGELTLEDFLEQMMMLRKMGPIGNLLGMLPGMGQMKEQ
                     IAQVDDKDVDRIAAIIRSMTPTEREDPKIINGSRRVRIAKGSGVTVAEVNSLVDRFFE
                     ARKMMKQMAGMGGLPGMGARRATKNVKKGKKGKGGRPGGRPGGAPPAALPPGLTRSLP
                     GGDGGLGLPAQLPPGMKMPDLSKFRFPPTGGPGNQGPAGPRPR"
BASE COUNT          555 a         1216 c         1138 g          584 t
ORIGIN      
        1 actcggggcg gccgcggtgg agcaggaggt cgcggcgggc actgtcatca tccgagaagg
       61 cgacgaggcc gacgcgctgt ggatcctcgt cagcggcaca ctcgcgatcc aggcgctcgg
      121 tcccgacggc agcacggtcg aattgcccga cgtcgacgcg ccgtcctacg tgggcgaact
      181 cgggctgctg cacggcaggc cgcgcagtgc cactgtcacc gtcaagcagg acgcgacctt
      241 gctgcgcatc gacggcaagg aattcctcaa cgcactcgag gcggcacctc cgagcgtctc
      301 cctgaagacg ctcgcgggcg tccgtctcgc acggacccct gcgcgcgacg acgtgcagga
      361 ctgacgtgcg agccttcttg tgcggagtgc gcgggtcgac tccggcgccg ggccccgact
      421 tcatccggta cggcggccac acgtcgtcgg tggctctggc gcacgatggc gagttgccga
      481 gcttggtaat cgacgccggc accgggttgc gacgtgtcac gaacctgctc gacggtgcgc
      541 ccttccaggg cagcatcgtg ttgagccatc tgcactggga tcacgtgcat ggcttgccgt
      601 tcttccaggg cggcgcaaga cacggacatc gggtcaacgt ctttctgccg gcgatcgacg
      661 gcgacgcggt tggcactctc tcgcgcggtt tgtcgcctcc gcacttcccg atcggaccga
      721 cccaactagg gaccggatgg acgttcgatc cactcgagga aggtccgcgt caactcgagg
      781 gcttcgatgt cgacgtccgt gagatcccgc acaagggcgg ccggacattc ggcttccggg
      841 tcaccgacgg tgccgggtcg cttgcctacc tcggcgacca tttcccgttg ggggaaggcg
      901 ccgggccgga cggtctcggt gcctatcacc ccgcggcgct cgcactcgcg cgcgacgtcg
      961 atgtgctcct gcacgacgcg caatttctgg ccgcacaatt tcccggcgtg acctacctcg
     1021 ggcattccgc ggtcgagtac gccatcggac ttgccaccag agcgggcgcc aaagcgctga
     1081 tgctcttcca ccacgcaccc gaccgcaccg acgacgagct cgacgcgatc gtgcagttgt
     1141 tcgcggacgc accggtgcgc gtcatcgcgg cgactgaggg caccgtcgca gctctgggca
     1201 tacctggcag ccccgtcgtc acagcactca gcactgcgaa gtagccgtca acgcacgaca
     1261 gtgccgcgga cgacgatgtg ccgggggctg cgcaacacgg tcaggtctgc tcgggggtcg
     1321 tcgtcgtaga cgacgaagtc ggccggggca ccttcggcca gccctggcag tccgagccag
     1381 cgccgtgctt tccacgaccc ggcagcgaga acgtcggcgt tggacaaacc ggccgcccgc
     1441 aatgccaaca tctcgtcgac gacgagcccg tgcggtagct gcccgccagc gtcggtgccg
     1501 acatagatcg gcacgcccgc ttcgtgcgcc gccgcaattc gcccgtcggc ggtacgtcgc
     1561 agcctgcgca ttctcgctgc gtacaacgga tacttcgtcg cttgatctgc gaacttgtca
     1621 tagttggcca cgttcactaa cgtcggcacg acggcgatgc cacgcgcgac gacatcacca
     1681 agagccttgt cgtcgaggcc ggtgccatgt tcgatcgagt cgacgccggc ggcaatcaga
     1741 tcgggcagtg cttcttcgcc gaacacgtgc acggccaccc gcgcacctgc gtcgtgcgcg
     1801 cgccgcaccg cctcggcgag gacatcggcc ggccaacacg gtgccaggtc gcctagatcg
     1861 cgatcgatcc aatcaccgac gagcttcacc caaggtgtgc cgtggccgcc tgacctgcga
     1921 gcctgcgctg ccgcggcgtc cggcaactgc tctggttcga gttcgatgcc atacccgcgg
     1981 atgtagcggc ggtttcgtgc gagatggcgt ccggcgtgca cgatgcgcgg cagatcaggt
     2041 tcggtatcga gctcctcgta ggtgtgcggc acaccggcat cgcgcagcag caacgcaccg
     2101 gcatcgcgat cggtgaccgc gtgtgcacgc gcctcctcga gcggcaccgc gccctcggcc
     2161 gccaatccga catggcagtg cgcgtcgacc aggccgggca acacgaatcc gtggtccgcg
     2221 aaggtgtcgc agtcggccgg ccgatcgaat gtgatgcgcc cacccacgac gaagaactcg
     2281 ccgacatccg cgtcgggcag cacggtggcc cggacgcgca gcgccgatgt cgtggcccac
     2341 atcggcgatg tcgtcgcgct cagcgcggcc tcggaccggc cggaccctgg ttgcccggac
     2401 cgcccgtcgg cgggaaccgg aacttcgaaa ggtctggcat cttcatgccc ggcggcagct
     2461 gggcaggcag gcccaagcca ccgtcaccgc caggcagcga ccgggtgagt ccgggcggca
     2521 aggccgcggg cggcgcaccg ccgggacggc cacccggcct gccacccttg cccttcttgc
     2581 ccttcttcac gttcttggtc gcgcgccgcg cacccatgcc cggcaggcca cccatcccgg
     2641 ccatctgctt catcatcttg cgagcttcga agaagcggtc gacgaggctg ttcacctcgg
     2701 cgacggtgac accggagccc ttggcgatgc gcacccggcg cgagccattg atgatcttcg
     2761 gatcttcacg ttcagtcggt gtcatcgagc ggatgatggc cgcgatgcgg tcgacgtcct
     2821 tgtcgtcgac ctgcgcaatc tgctctttca tctgccccat cccgggcagc attccaagca
     2881 ggttgccgat cgggcccatc ttgcgcagca tcatcatctg ctcgagaaag tcttcgagtg
     2941 tgagctcgcc ttcggtgagc ttgcgtgcgg tctcctcggc ctcgccggcg tcgtacgcgc
     3001 tttgggtctg ctcgatcagc gtgaggatgt cgcccatgcc gagaatgcgt gaggccatcc
     3061 gctccggatg gaatgtgtcg aagtcctcga gcttctcacc ggtactggcg aacatgatct
     3121 gccggccggt cagcgatgcc accgacaagg cggcaccacc gcgcgcgtcg ccgtcaagct
     3181 tggtcagcac gacaccgtcg aagccgacac cgtccaggaa ggcctgggca gtgttgacgg
     3241 cgtcctggcc gagcatggcg tcgacgacga acaagatctc gtttgggtcg acggcatcac
     3301 ggatgtcgat cgcctgctgc atcatctcgg catcgacgcc gagacggccg gccgtgtcga
     3361 ccagcacgat gtcgtgaccc agccgcttcg catgctcgat ggcggccttc gacacagcaa
     3421 cgggatcgcc gacgccgctt ccgggttcgg gtgcgaagac ggggacgcca gcctgcttgc
     3481 cgagcacctg gag
//
LOCUS       DATCBJ010000506         2047 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_12069, whole genome shotgun sequence.
ACCESSION   DATCBJ010000506 DATCBJ010000000
VERSION     DATCBJ010000506.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2047)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2047)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2047
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_12069"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            169..1434
                     /locus_tag="VII50_10295"
     CDS             169..1434
                     /locus_tag="VII50_10295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003512473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HEY5051280.1"
                     /translation="MALDQSALLELLEVLKAADAGDVVRRAVESVFQALIDAEATAAI
                     GAGPHQRTDARVAQRNGYRDRLATTAAGDVELRIPKLRAGSFFPSLLERRRRIDQALF
                     AVVMEAYVTGTSTRKVDDLVVAMGADSGISKSEVSRICADLDTEVATFRDRSLAECEF
                     PYVFLDATFCKARIGGDRRGRGSRVAAQAVVIATGVSADGRREVLGFDVGDSEAGAFW
                     TAFLRSLKTRGLAGVQLVISDAHTGLKAAVGATLLGASWQRCRVHFGRNLLAAVPKGH
                     GDMVAAAVRTIFAQPDAGSVRAQLDTIAGMLGRQFPKVETMLRDAADDITAFADFPAA
                     HWKKIWSTNPLERVNKEIKRRTDVVGVFPNPASLLRLAGAVLAEIHDEWQVNDRRYLS
                     EASMALLTQQEDEPTPTDHPVALPAQIAS"
     gene            complement(1630..>2047)
                     /locus_tag="VII50_10300"
     CDS             complement(1630..>2047)
                     /locus_tag="VII50_10300"
                     /inference="COORDINATES: protein motif:HMM:NF013561.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HEY5051281.1"
                     /translation="TSPPPGDGVRQIFHGIWAKALGGRPSIDPDAKPGQHARVRQVRV
                     SAEVDARIEILAADRHVKASDIIREALAAYLASAVSPGEKGGSRTRRPRAAVPAKDAR
                     GRAPAKAVPAVAAKRVSVSAKTARTRSGVAAKRLAK"
BASE COUNT          339 a          684 c          668 g          356 t
ORIGIN      
        1 gtccggttgg cacaccgtga cgacacaacc gccggtcctt ccgcttgcgg tagagggtca
       61 cgtcccccga agtggtgtaa gtagcggccg gtctcctgaa cgttaggggg ccatcgtgtg
      121 agtctttgcg agatccgacc aaggaaactc gcgaaggaga tgcacacgat ggccctggac
      181 cagtctgcac tgttggagtt gcttgaggtg ttgaaggccg ccgatgccgg tgatgtggtg
      241 cgccgtgcgg tggagtcggt gttccaggcg ttgatcgatg cggaggcgac cgcggcgatc
      301 ggcgccggac cgcatcagcg caccgacgcg cgggtcgcgc aacgcaacgg gtaccgcgat
      361 cggctggcca ccaccgcggc aggcgatgtg gagttacgga ttcccaagct gcgtgccggg
      421 tcgttcttcc cgtcgctgct ggaacggcgc cggcgcatcg atcaggcgct gttcgcggtg
      481 gtgatggagg cctacgtcac cggcacctcg acgcgcaagg tcgatgacct ggtggtcgcg
      541 atgggcgccg attcggggat ctccaaaagc gaggtgagcc ggatctgcgc ggacctggac
      601 accgaggtcg cgacgtttcg ggaccggtcg ctggccgagt gtgagttccc ttacgtgttt
      661 ttggatgcca cgttctgcaa agcccgcatc ggtggggacc ggcgcgggcg cggcagccgc
      721 gtcgccgccc aggcggtggt gatcgccacc ggggtatctg ctgacgggcg ccgggaagtc
      781 ctggggtttg acgtcgggga cagtgaagct ggcgcgtttt ggaccgcgtt cctgcggtcg
      841 ttgaaaaccc gcggtctggc cggggtgcag ctggtcatct ccgacgcgca caccggcctg
      901 aaagccgctg tcggggcgac cctgctcggc gcgagctggc aacgctgccg cgtgcacttc
      961 ggacggaact tgctcgccgc ggtgcctaaa ggccacggcg acatggtcgc cgccgcggtg
     1021 cgcactatct tcgctcaacc cgatgcggga agtgtccgcg cgcaactgga caccatcgcc
     1081 ggcatgctcg gccggcagtt ccccaaagtc gagacgatgc tgcgcgacgc cgccgacgac
     1141 atcaccgcgt tcgccgattt ccctgctgcg cattggaaga agatctggtc gaccaaccca
     1201 ctcgagcgcg tcaacaaaga aatcaaacgc cgcaccgacg tcgtcggggt gttccccaac
     1261 cccgcatccc tgctccgcct cgccggcgcc gtgctcgccg agatccacga cgaatggcaa
     1321 gtcaacgacc gccgctacct ctccgaagca tccatggcat tgctgaccca acaagaagac
     1381 gaaccgaccc cgaccgatca cccagtagcg ctgccagcgc agatcgcgtc atagttcaac
     1441 cggcacccgc caccgcgaag acacacacgc gacgacttac accactcggc gggacgtgac
     1501 cttttcatcg tgttgtcgcc gtcgggtgaa tagcgttggc tggttttgac tgtcgtgttg
     1561 cccaacggtg atcaacttct cccccaccga tctcacccga cctggtcgca gctacgcggt
     1621 cttgacggct cacttggcga gcctcttggc tgcgacaccg ctccgcgttc gggccgtctt
     1681 tgcgctcacc gatacgcgct tggctgcaac tgccgggaca gccttcgccg gcgctcggcc
     1741 acgggcatct ttggcaggaa ccgcggcccg ggggcggcga gttcgtgacc cgcccttctc
     1801 ccctggactc accgcactgg caaggtaggc cgccaacgcc tcccggatga tgtcactcgc
     1861 cttgacatgg cgatctgcgg ccaggatctc aatgcgggca tcaacctcgg cagagacccg
     1921 gacctgccgg acccgcgcgt gctgacccgg cttcgcgtca gggtcgatcg aaggccgacc
     1981 acccaacgcc ttcgcccaga tgccgtggaa gatctgccgt acaccgtcgc caggtggcgg
     2041 cgacgtg
//
LOCUS       DATCBJ010000507         2861 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_153270, whole genome shotgun sequence.
ACCESSION   DATCBJ010000507 DATCBJ010000000
VERSION     DATCBJ010000507.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2861)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2861)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2861
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_153270"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1445
                     /gene="ftsH"
                     /locus_tag="VII50_10305"
     CDS             <1..1445
                     /gene="ftsH"
                     /locus_tag="VII50_10305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006680774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HEY5051282.1"
                     /translation="GTGKTLLARAVAGEAGVPFYAISGSDFVEMFVGVGASRVRDLFE
                     QGKKHAPCIIFIDEIDAVGRHRGAGLGGGHDEREQTLNQLLVEMDGFDVKGGVILIAA
                     TNRPDILDPALLRPGRFDRQIVVDRPDMEGRKGILRVHAKGKPFEGDVDLDVIARRTP
                     GFTGADLANVINEAALLTARHDRKQIDMLTLEEAIDRVMAGPERKSRIMGEKEKKIIA
                     YHEGGHALVGHALPNADPVHKVTILPRGRALGYTLSLPTEDKFLVTRAEMMDQLAMLL
                     GGRTAEELVFHEPTTGAANDIEKATSIARSMITQYGMSEKLGARKFGQENGEVFLGRD
                     MGHQRDYSEEIASTIDDEVRRLIESSHDEAWEILVEYRDVLDDLVLELMEKETLQKDE
                     VLRIFARVQKRPHRGSYTGYGKRQPSEKAPVLTPREVAVLASGGADSGHTNGLSSNGG
                     SGSVGSGTSIHSDSDPVIDGSTFDGPPHSE"
     gene            1442..2011
                     /gene="folE"
                     /locus_tag="VII50_10310"
     CDS             1442..2011
                     /gene="folE"
                     /locus_tag="VII50_10310"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020664250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0042559 - pteridine-containing compound
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HEY5051283.1"
                     /translation="MTVDLEKIEAAVREILVAIGEDPTRDGLTQTPARVARAMAEQFA
                     GLGQRASDVLTTVFDADHDEMIIVKDIEIYSTCEHHLVPFHGVAHVGYIPNVNGEITG
                     LSKIARLVDLFAKRPQVQERLTSQIADAMQEILEPRGVIVVVECEHLCMSMRGVRKPG
                     ARTVTSAVRGAFRESDRTRAEALRLIAGR"
     gene            2207..>2861
                     /locus_tag="VII50_10315"
     CDS             2207..>2861
                     /locus_tag="VII50_10315"
                     /inference="COORDINATES: protein motif:HMM:NF028305.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5051284.1"
                     /translation="MAGVLTFLLGVRDIAMVVRPHLWERVKVINHVLPGNIDPAVVHG
                     AVGAALVVAGALLILLSHALRRRKRRAWRAVVAILLVTATLHLLHGPFTWRETVDWLP
                     LDAVFVIALVLYRKEFFAVGDPRTRWLALWAFLGLTAGSFVLGALLIEVRPDEVIGSA
                     GFLAVAQHVLYGLVGVSGPIHFHSDRSTDLIAGTLASMGAFTALTTVYLLFRPAEPRG
                     "
BASE COUNT          485 a          946 c          959 g          471 t
ORIGIN      
        1 ccggtaccgg caagaccctg ctcgcccgcg ccgtcgccgg cgaggccggg gtgccgttct
       61 acgcgatctc cgggtcggac ttcgtcgaga tgttcgtcgg cgtcggcgcg agccgcgtgc
      121 gcgacctctt cgagcagggc aagaagcacg cgccgtgcat catcttcatc gacgagatcg
      181 acgccgtcgg ccggcaccgc ggcgcgggcc tcggcggtgg ccacgacgag cgtgagcaga
      241 cactgaacca gctcctggtc gagatggacg gcttcgacgt gaagggcggc gtgattttga
      301 tcgccgcgac caaccgtccc gacatcctcg acccggcgtt gctgcgccca ggccgcttcg
      361 accggcagat cgtcgttgac cgccccgaca tggagggccg caagggcatc ctgcgggtgc
      421 acgccaaggg caagccgttc gagggtgacg tcgacctcga cgtcatcgcg cggcgcacac
      481 ccgggttcac cggcgccgac cttgcgaacg tcatcaacga ggcggcgctg ctgaccgccc
      541 gtcacgaccg caagcaaatc gacatgctca ccctcgagga ggccatcgac cgcgtcatgg
      601 ccggccccga gcgcaagagc cgcatcatgg gtgagaagga aaagaagatc atcgcctacc
      661 acgagggcgg ccacgctctc gtcggtcacg cgttgccgaa cgccgacccg gtgcacaagg
      721 tgacgatcct gccccgcggc cgcgcgctcg ggtacacact ctcgctgccg accgaagaca
      781 agttcctggt cacccgcgcg gagatgatgg accagctcgc gatgctgctt ggcggccgca
      841 ccgcggagga gctcgtcttt cacgagccga cgaccggtgc cgcgaacgac atcgagaagg
      901 cgacgtccat cgcgcgcagc atgatcaccc aatacggcat gagcgagaaa ctgggcgcgc
      961 gcaagttcgg tcaggagaac ggagaggtgt tcctcggtcg cgatatggga caccagcgcg
     1021 actactccga ggagatcgcc tccacgatcg acgacgaggt gcgccggctc atcgagagct
     1081 cgcacgacga ggcgtgggaa atcctcgtcg agtatcgcga cgtgctcgac gatttggtgc
     1141 tcgagctgat ggagaaggag acgctgcaaa aggacgaggt gcttcggatc ttcgcgcgcg
     1201 tccaaaagcg gccacaccgc ggttcctaca ccggctacgg caaacgccag ccatcggaga
     1261 aagcgcccgt cctgactccc cgcgaagtcg ccgtcctggc ctccggcggc gccgacagtg
     1321 ggcacaccaa cggcctgtct tccaacggtg gatcgggcag cgttggatcg gggacttcga
     1381 tccattccga ctccgacccg gtgatcgacg gctccacctt tgacggtccg ccgcactcgg
     1441 aatgacggtc gacctagaga agatcgaggc cgccgtccgc gaaatcctgg tcgccattgg
     1501 cgaggatccc acccgagacg ggctgaccca gacccccgcg cgggttgcca gggcgatggc
     1561 agaacagttc gccggactgg ggcagcgcgc gtcggacgtg ctgacgacgg tgttcgatgc
     1621 cgaccacgac gaaatgatta tcgtgaagga catcgagatc tacagcacct gcgagcatca
     1681 cctcgttccg ttccacgggg tcgcccacgt cggttacatc ccgaacgtca acggcgagat
     1741 caccgggctg tcaaaaatcg cccggctggt tgacctgttt gcgaagcgcc cccaggtcca
     1801 ggagcgtttg acctcgcaga tcgccgatgc gatgcaggag atcctcgagc cgcgaggggt
     1861 catcgtcgtg gtggagtgcg agcacctgtg catgtcgatg cgaggggttc gcaaacccgg
     1921 tgcgcggacg gttacgtcag ccgtccgtgg ggcgttccgc gagtcggatc ggacgagggc
     1981 ggaagccctg cgcttaattg cgggccgctg acccggcaag ctgccgatag acctatggga
     2041 gccctgcccg cctactctta agtttgtgac tggagatccg aaccccgtgc gtgtgaagac
     2101 cgctgagaag acggccgttc ccggcgggcg acccgttgca gccccgcacg ctccggtcct
     2161 accggtcggg aaggagcggc ggtcagatcg gcgttgggtc cctctcgtgg ccggcgtctt
     2221 gacgttcctc ctcggcgtgc gtgacatcgc catggtggtc cggccgcacc tgtgggagcg
     2281 cgtcaaggtc atcaaccacg tgctgcccgg caacatcgac ccagcggtcg tgcacggcgc
     2341 cgtcggcgcg gcgctggtgg ttgcgggcgc gttgctcatc ttgctctcgc acgcgcttcg
     2401 ccgccgcaaa cgacgggcgt ggcgagcggt ggtggcgatc ctcctcgtaa ccgcgacgct
     2461 gcacctcctg cacggcccgt tcacctggcg ggagaccgtc gattggttgc cactcgatgc
     2521 agtgttcgtc atcgcgctgg tgctataccg caaggagttc ttcgcggtcg gagatccgcg
     2581 cacccggtgg ctggcgttgt gggcattcct cggcctgacc gccggcagct tcgtgctcgg
     2641 cgcgctgctg atcgaggtac ggccggacga agtgatcgga tcggccgggt tcctcgccgt
     2701 cgcgcagcac gtcctgtacg ggttggttgg ggtctctggg cctatccact ttcacagcga
     2761 cagatcgacc gacctcattg cggggacgct cgccagcatg ggcgcgttca ccgcgttgac
     2821 cacggtctac ctgctgtttc ggccggccga gccgcggggg a
//
LOCUS       DATCBJ010000508         3481 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_527964, whole genome shotgun sequence.
ACCESSION   DATCBJ010000508 DATCBJ010000000
VERSION     DATCBJ010000508.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 3481)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3481)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3481
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_527964"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..205
                     /locus_tag="VII50_10320"
     CDS             <1..205
                     /locus_tag="VII50_10320"
                     /EC_number="2.1.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050797 - thymidylate synthase (FAD)
                     activity [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent thymidylate synthase"
                     /protein_id="HEY5051285.1"
                     /translation="TFSSMYVTCNARSLMALLSLRTKHEVSTFPSFPQREIEMVAEKM
                     EAEWAALMPLTHAAFEAHGRVSP"
     gene            247..1155
                     /gene="dapA"
                     /locus_tag="VII50_10325"
     CDS             247..1155
                     /gene="dapA"
                     /locus_tag="VII50_10325"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HEY5051286.1"
                     /translation="MATPDSATTAPPPFGHVLTAMVTPFTADGGLDLDGAQQLASYLV
                     DHGNDGLVINGTTGESPTTTDEEKDRLLRAVLEAVGDRAKIVAGVGTSDTRHTIELAH
                     GAEKAGAHGLLVVTPYYSKPPQAGLLAHFGQIADATGLPNMLYDIPGRTATPIETETL
                     VRLAEHDKIVAVKDAKGDLEGTSWVLRRTDLAYYSGDDKMTLPLLSIGAVGVVGVPTH
                     LFGAETGAMIAAYLRGDVAGALAAHRQLLPVFCGFFRTQGVILTKAALRLAGLPGGPV
                     RPPLVDATDEQIEQLRRDIADARTPA"
     gene            1152..2837
                     /locus_tag="VII50_10330"
     CDS             1152..2837
                     /locus_tag="VII50_10330"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HEY5051287.1"
                     /translation="MSYPHTGMAAPGPLADNGLRIVALGGLGEVGRNMTVFEYGGRLL
                     IVDCGVLFPDPEQPGIDLILPDFDYIRSRLDKIEAIVLTHAHEDHIGAIPYLLRERPD
                     IPLVGSNLTLAFIEAKLVEHRIKPYVLAVKEGQRERLGAFDCEFLAVNHSIPDALAVA
                     IRTPAGLVLHTGDFKMDQLPLDGRLTDLGGFARLGAEGIDLLMSDSTNAEVPGFVTSE
                     REITPVLRRVFEGAKRRVIVACFASHVHRVQQVLDVAVENRRKVAFVGRSMVRNMGVA
                     RDLGYLRVPADLIVDAREIDAYPPEQVVLISTGSQGEPLSALSRMAGGDHPVRIEQGD
                     TVVLASSLIPGNETSVFRVVNGLTRWGATVVHKSNALVHVSGHAAAGELLYVLNLTRP
                     SNFMPIHGEWRHLQAHAAIARLSGVDTDRIVIADDGHVVDLVDGRASVTGAIRCGYVY
                     VDGLSVGEVTETSLKDRRILGEEGFVTVVVVLDSATGKLAGGPEIHARGSGIDDAALD
                     GVRSKVQEALEKSAADGMSDPHQLEQVVRRVVGRWVNDAYRRRPMILPVVLEV"
     gene            3122..>3481
                     /locus_tag="VII50_10335"
     CDS             3122..>3481
                     /locus_tag="VII50_10335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HEY5051288.1"
                     /translation="MIFAHLIGATSRAIGAKARDLDPAHRRDGAGLAALGAALVIAGG
                     VWWRLPGPIGTVIVAVVRGAVGSGAVVVPVLLAALAWRTLRHPDRASASGRIVIGWGA
                     VTLGVLGLLHVAHGSPDP"
BASE COUNT          520 a         1222 c         1156 g          583 t
ORIGIN      
        1 gacgttctcc tcgatgtacg tcacctgcaa tgcgcgctca ctgatggcgt tgctgtcgtt
       61 gcgcaccaag cacgaggtga gcaccttccc gagcttcccg cagcgcgaga tcgagatggt
      121 cgccgagaag atggaagctg agtgggctgc cctgatgccg ctcacccacg ccgcgtttga
      181 agcccacggg cgggttagcc cgtaacggga ttcgtcgcgg ggcgccgtac cggtaccgtt
      241 gcaaccatgg ccaccccgga ctcggctacc accgcaccac cacctttcgg acatgtgctc
      301 acggcgatgg tgaccccatt caccgccgac ggcgggctcg acctcgacgg cgcccagcag
      361 ttggccagct atctcgttga ccacggcaat gacggcctcg tcatcaacgg cacgaccggt
      421 gaatcaccga cgaccaccga tgaggagaag gaccggctgt tgcgggcggt gctcgaagcc
      481 gtcggcgacc gggccaagat cgtcgcgggc gtggggacaa gtgacacccg gcacacgatc
      541 gagctcgccc acggcgcgga gaaggcgggc gcgcacgggc tcctggtcgt cacgccgtac
      601 tactccaagc caccccaggc cggcctgctg gctcacttcg gccaaatcgc cgatgccacg
      661 gggctgccga acatgctcta cgacatcccc gggcgcactg ccacgccgat cgagaccgag
      721 acactcgtcc ggctcgcgga gcacgacaag atcgttgcgg tgaaggacgc caagggcgac
      781 ctcgagggca cctcgtgggt gctgcgtcgc accgaccttg cttattactc cggcgacgac
      841 aagatgacgc ttccgctgct gtcgatcggg gcggtcggcg tggtcggcgt cccgactcat
      901 ctcttcggtg ctgagacggg cgcgatgatc gccgcgtacc tgcggggcga cgttgcaggc
      961 gcgctcgccg cccatcgcca gctgctcccg gtgttctgcg gatttttccg cacgcagggc
     1021 gtcatcctca cgaaggcagc cttgcggctg gccggactcc ccggtggtcc ggtgcgcccg
     1081 ccgctggtcg acgcgacgga cgagcagatc gaacagttgc gccgcgacat cgcggacgct
     1141 cggacgcccg cgtgagctat ccgcacaccg gcatggccgc gcccggtcca cttgccgata
     1201 acggtcttcg gattgtcgcc ctcggtggcc tcggcgaggt cggtcgcaac atgaccgtct
     1261 tcgaatacgg cggccggttg ctcatcgtcg actgcggcgt gctcttcccc gatcccgagc
     1321 agcccggcat cgacttgatt ctgccggact tcgactacat cagatcgcgg ctcgacaaga
     1381 tcgaggcgat cgtgctcacc cacgcgcacg aggatcacat cggcgcgatc ccgtacctgc
     1441 tgcgcgaacg gcccgacatt ccgctggtgg gttcgaacct gacgctcgcc ttcatcgaag
     1501 ccaagctggt cgagcaccgc atcaagccct atgtgctcgc cgtcaaagaa ggccaacgcg
     1561 agcgactggg cgctttcgac tgcgagttct tggccgtcaa ccattcgatc cccgacgcgc
     1621 ttgcggtggc gatccgcaca ccggccgggc tcgtgctgca caccggcgac ttcaagatgg
     1681 accagctgcc acttgacggt cggctcaccg acctcggcgg cttcgcgcgg ctgggtgccg
     1741 agggcatcga cttgctgatg tccgactcga ccaacgccga ggtacccgga ttcgtcacct
     1801 ctgaacgcga gatcaccccg gtactgcgcc gagtcttcga aggcgctaag cggcgcgtca
     1861 tcgtcgcgtg ttttgcgagc cacgtgcacc gcgttcagca ggttctcgac gtcgctgtcg
     1921 agaaccgtcg caaggtcgcc ttcgtgggcc gctcgatggt tcgcaacatg ggcgtcgccc
     1981 gtgacctcgg atatctgcga gtgcctgctg acctcatcgt cgatgcccgc gagatcgatg
     2041 cctacccgcc cgagcaggtt gtcctcatct cgaccggctc gcagggcgag ccgctgtcgg
     2101 ctctctcccg gatggccggc ggcgatcacc cggtccggat cgagcagggc gacacggtcg
     2161 tactggcgag ttcactgatc cccggcaacg agacgtccgt ctttcgcgtc gtgaacggct
     2221 tgaccagatg gggcgccacc gtcgtgcaca agagcaacgc gctggtccac gtgtcagggc
     2281 acgccgctgc cggcgaactg ctctacgtgt tgaacctgac gcggcccagc aacttcatgc
     2341 cgatccacgg cgagtggcgc catcttcagg cgcacgccgc gatcgcccgg ctctccgggg
     2401 tcgacaccga tcgcatcgtg atcgccgacg acggccatgt cgtcgacctg gtcgacggtc
     2461 gggcttcggt cacgggcgcc atcaggtgcg gctacgtcta cgtcgacggc ctgtcggtgg
     2521 gcgaggtgac ggagacctcg ctgaaggacc gccgaatcct gggcgaagaa ggcttcgtga
     2581 cggtcgtcgt cgtccttgac tcggcgaccg gcaagctcgc cggtggccca gagatccacg
     2641 cccgcggctc ggggatcgac gacgcggcac tggacggcgt tcggtcgaag gtccaggagg
     2701 cgctggagaa gtcggccgcc gacggcatga gcgacccgca ccagctcgaa caggtcgtgc
     2761 ggcgagtcgt tggccgctgg gtcaacgatg cctaccgtcg gcggccgatg atcctgccgg
     2821 tggtgctcga ggtctgagcg aggcgtctgc gccccggtcg cgacacggcg gcctgaaggc
     2881 atgtgactac tgaggctggt ggggtgttcc cggtagcctg acgaccatgg cgacccgtgc
     2941 ccccaatcgg cccccctcga gtccgggtgc gcgcccgcgc accggcggac gcccgccggc
     3001 ccggcgtcca gcgcctccgc gccgtaggcc tcgcgcccgc cggccccgta gccgcgggcg
     3061 tctcgccgca ctcggcggcg gcctgttccg cgcactaggg cggctgctgc gcggcgcgtg
     3121 gatgatcttc gcccacctga ttggtgcgac ctcccgagcg atcggtgcca aggcccgcga
     3181 cctcgatcct gcgcaccgcc gcgacggtgc cgggcttgcc gccctgggcg cagcgctcgt
     3241 catcgcaggc ggggtctggt ggcggcttcc tggccctatc ggcaccgtga tcgtcgcggt
     3301 cgtgcgcgga gccgttggct ccggcgcggt ggtcgtgccg gtactcctcg cagcgctggc
     3361 atggcgaact ctgcgtcacc ccgaccgtgc gtcggcaagc ggccgcatcg tgatcggctg
     3421 gggtgctgtc acgctcggcg tcctcggcct gctgcatgtc gcccacggca gccctgaccc
     3481 g
//
LOCUS       DATCBJ010000509         3653 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_529944, whole genome shotgun sequence.
ACCESSION   DATCBJ010000509 DATCBJ010000000
VERSION     DATCBJ010000509.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 3653)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3653)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3653
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_529944"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..489
                     /locus_tag="VII50_10340"
     CDS             <1..489
                     /locus_tag="VII50_10340"
                     /inference="COORDINATES: protein motif:HMM:NF025064.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5051289.1"
                     /translation="GDLLTRSGAYIVEAAELIELGASELVVLVEEAGSLADRVRSAPS
                     MAGVPAHVIEIAAPVQFLSAMAAHDPHRSLAEDAAAMGAAASSTRCAVVGPAADGALC
                     DDAVSAISRLLAGGGDLVTVVGGEGIGARIAEQLATIRPDIDVNHLSVDTLPSVVWLG
                     VE"
     gene            489..2744
                     /locus_tag="VII50_10345"
     CDS             489..2744
                     /locus_tag="VII50_10345"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF016724.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HEY5051290.1"
                     /translation="MVDLDAPLRPSVGVIAAILGEKLELHTVGDLVRHYPRRWDKRGE
                     LTDFRDLVPGEYATIFAIISKVTSKRVTTKTNKSLNKTDVTVTDGSRKLVLTFFNQKI
                     WQQNRFPEGAGAYFSGKVEVFNGKLQLVNPEVDIVEDDDVVGRADFAGEILAVYPAVK
                     GADTVKIRRCMRLVLDTLDLPEDPVPQEIRDKLELIGYREALEGIHRPKTFDERDGAR
                     GRLAWDEAFVLQIVLAQRRVAAAGLPATARRPVNDGLLAAFDARLPFELTNGQRSIGD
                     VIAAEMAADHPMHRLLQGEVGSGKTVIALRAMLAVIDAGGQAALLAPTEVLAQQHLRS
                     ITDMLGDLALAGQLGGAEKATTVTLLTGSLSQAARRKAMLDAASGAAGIVIGTHALLE
                     EKVQFADLGLVVVDEQHKFGVEQRDALRAKARDDRRPHVLVMTATPIPRTVAMTVYGD
                     LEVSTLDELPKGRAPISTAVAPLAGKPEWAERVWQRVRDEVAAGHQAYVVCPRIGGDE
                     PDDSDAPDEDPLDADDSDSDALNFEAAGESDLARGRSAPMRPPIGVLELAPQLADGYL
                     RDLRVEILHGRMPSEAKDATMRAFADGAVDVLVATTVIEVGVDVGNATIMVIMDAERF
                     GVSQLHQLRGRVGRGSAPSRCLLVTELPAGHPGRARLDAVAATSDGFELSELDLTLRR
                     EGTILGGQQHGRSDLKLLSLVRDRDLIGTAREQATRIVEADPELLDHRPLAAAVKRLV
                     ATEDAEFLEKA"
     gene            2774..3217
                     /locus_tag="VII50_10350"
     CDS             2774..3217
                     /locus_tag="VII50_10350"
                     /inference="COORDINATES: protein motif:HMM:NF013412.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HEY5051291.1"
                     /translation="MVEPVAWSDVVARFGAARNAWFGTVDASGAPHSVPIWTASVDDV
                     LYVFGERRSVKFRNIAANPRVVVNLESGDEVVIVRGSAYDVGSPAQVPNVVAAFAAKY
                     TEPDDVQWLPEVDPSVDLVARIAPSVALLWSSDDYFDTLRRWQSG"
     gene            3226..>3653
                     /locus_tag="VII50_10355"
     CDS             3226..>3653
                     /locus_tag="VII50_10355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009074198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmD family RNA methyltransferase"
                     /protein_id="HEY5051292.1"
                     /translation="MAGQAGGRRLAVPPSGTRPTSDRAREALFSSLESSLHGLAGIRL
                     LDLYAGSGAVGLEAWSRGASRVVLVESAAAAVKVLRVNVTNLRSSREPGPVGKIEVVA
                     AKAERTAADLPTSGFDVVFADPPYDLPAVQVAGVLATLAQR"
BASE COUNT          586 a         1186 c         1273 g          608 t
ORIGIN      
        1 ggcgacctgc tgacgcggtc cggcgcgtac atcgtcgagg ccgccgagct gatcgagctg
       61 ggcgcgtcgg agctggttgt cctcgtcgaa gaggccggga gcctggccga tcgtgttcgc
      121 agcgcgccga gcatggctgg ggtgccggcc cacgtgatcg aaatcgcggc gccggtgcag
      181 ttcctttcag cgatggccgc gcatgacccg caccgatcgc tcgccgagga cgcggccgcg
      241 atgggggctg ctgccagcag cactcgctgc gcagtcgtcg gtccagcggc cgacggcgcg
      301 ctgtgtgatg acgcggtctc tgcgatatcc cggttgctcg ccggcggcgg tgacctggtc
      361 actgtggtcg gtggggaggg gatcggcgcg cgcatcgcag agcagctggc aaccatccgg
      421 ccggacatcg acgtgaacca cctgagcgtc gacaccctgc cgtcggtggt ctggctgggg
      481 gtggagtgat ggtcgacctc gacgcacctt tgcgaccgag cgtcggcgtg atcgccgcga
      541 tcctgggcga gaagctcgag ctgcacaccg tcggtgacct ggtgcggcac tatcccaggc
      601 gttgggacaa gcgtggggag ctgaccgact tccgtgatct ggttccaggc gaatacgcga
      661 cgatcttcgc gatcatttcg aaggtgacca gcaagcgtgt cacgacgaag accaacaaga
      721 gtctcaacaa gaccgacgtg acagtaactg acggaagccg gaagctcgtg ctcacgtttt
      781 tcaatcagaa gatttggcag cagaaccgtt tcccggaagg ggcaggcgcg tacttctcgg
      841 gcaaggtcga ggtgttcaac ggcaagttgc aactcgtcaa tcccgaagtc gacatcgtcg
      901 aagacgacga tgttgtcggt cgcgccgact tcgcaggcga gatcctggcg gtctacccag
      961 ccgtcaaagg cgcagacacg gtgaagattc gtcgctgcat gcggctcgtg ctcgatacgc
     1021 tcgacctgcc ggaagatcca gtgccgcaag agattcgcga caagctcgag ctcatcggat
     1081 accgagaggc actcgaagga atccaccgac cgaagacctt cgatgagcga gatggcgctc
     1141 gcggccgcct cgcctgggac gaagcgttcg tcttacaaat cgtcttggca caacgccggg
     1201 tggccgccgc cgggttgccg gccaccgctc ggcgtccggt caatgacggc ttgcttgcgg
     1261 ctttcgatgc gcgactgccg ttcgagctta caaacggtca gcgcagcatc ggcgacgtga
     1321 ttgccgccga gatggcggcc gaccatccga tgcaccggct gttgcagggc gaggttggct
     1381 cgggaaagac ggtcattgca ctacgcgcca tgctggcggt tatcgatgcc ggagggcagg
     1441 cggcgctgct cgcaccgacc gaagtgctgg cgcagcagca tcttcgctcg atcaccgaca
     1501 tgctcggcga tcttgcactg gccggccaac tcggcggcgc agagaaggca accaccgtca
     1561 cgttgctgac cggatcgctc tcgcaagcgg ctcgacgaaa ggcaatgctc gatgctgcgt
     1621 ccggtgcagc cggcatcgtc atcggcacgc acgcactgct cgaggagaag gtgcagtttg
     1681 ccgatctcgg cctggtcgtc gtcgacgagc agcacaagtt cggcgtcgag cagcgcgatg
     1741 cgctgcgtgc aaaggcgcgc gatgaccggc ggccgcatgt tctcgtgatg acggccaccc
     1801 cgatcccacg gaccgtcgcc atgaccgtgt acggcgacct cgaggtttct accctcgacg
     1861 agttgccgaa gggccgcgcg ccgatctcga cagcggtggc gccgcttgct gggaagcctg
     1921 agtgggccga gcgggtgtgg cagcgagtgc gcgacgaggt ggccgccggc caccaggcct
     1981 acgtcgtgtg cccccggatc ggcggcgacg aacccgatga ctccgacgcg cccgatgagg
     2041 atccgctgga cgccgacgac tccgactccg acgccctcaa cttcgaggca gccggagagt
     2101 cggatctggc tcgtggccgc agtgcgccga tgcgcccgcc gatcggcgtc ctcgaactgg
     2161 ctccgcagct tgccgacggt tacttgcgcg acctgcgggt cgagatcctg cacgggcgaa
     2221 tgcccagtga ggccaaggac gcgacgatgc gcgcgtttgc cgacggcgcc gtcgatgtgc
     2281 tcgttgcgac gaccgtcatt gaggtcgggg tcgacgtcgg gaacgcgacg atcatggtga
     2341 tcatggacgc cgagcggttc ggcgtctcgc aacttcacca gctgcgtggc cgggtcggtc
     2401 gtgggtccgc gccgagccga tgcctgctcg tcaccgaact ccctgcaggg cacccgggtc
     2461 gcgcccgact cgacgccgtc gccgcgacgt ccgacggttt cgagctgtcc gaactcgatc
     2521 tcaccctgcg acgtgaggga accatcctcg gcggacagca gcacggcagg agtgacctga
     2581 agctgctgtc gttggtgcgc gatcgcgacc tgatcggcac ggcccgcgaa caggcaactc
     2641 gcatcgtcga ggccgatccc gaactgctgg accaccggcc gctggccgcc gcggtcaagc
     2701 ggctggttgc caccgaggac gcggagttcc tcgaaaaagc ctgaagtctc gcaccgctgc
     2761 aatgatgacg cccatggtcg agcctgtggc gtggagcgac gtcgtcgccc gctttggcgc
     2821 cgctcgcaac gcctggttcg gcaccgtcga cgcatccggt gccccgcatt ccgtgccgat
     2881 ctggacggcg tcggtcgacg acgtgctgta cgtcttcggc gagcgccggt cggtgaagtt
     2941 ccgcaacatc gccgccaatc cccgggtggt cgtgaacctc gagagcggag acgaggtcgt
     3001 gatcgttcgc ggcagcgcgt acgacgtggg ctcgccggcg caggtcccga acgtcgtcgc
     3061 cgcattcgcc gcgaagtaca ccgagccgga cgacgtgcag tggctgcctg aggtcgaccc
     3121 cagcgtggat ctggtggccc gcatcgcgcc atccgtcgcc ctgctctgga gcagcgacga
     3181 ctacttcgac accttgcgcc gttggcagtc ggggtgactc ggatcgtggc cgggcaagcg
     3241 ggcgggcggc gcctcgcggt accgccgtcc ggaacccggc cgacgtccga ccgtgcccgc
     3301 gaggcgctgt tctccagcct cgagtcatcg ctgcacggac tcgcgggcat ccggctgctc
     3361 gatctgtatg ccggctccgg tgcggtcggg cttgaggcgt ggtcgcgcgg ggcatcgcgc
     3421 gtcgtcctgg tcgaatcggc cgcggcggcg gtgaaggtgc tgcgggtgaa cgtcactaac
     3481 ctgcgctcgt cccgtgagcc ggggccggtg ggcaagatcg aggtggtggc ggccaaagcg
     3541 gagcgaacgg cggccgatct cccgacgtcc ggcttcgacg tcgttttcgc cgatccgccc
     3601 tacgatctgc ccgcggttca ggtcgccgga gtgctggcga cgctggcgca gcg
//
LOCUS       DATCBJ010000510         4088 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_347757, whole genome shotgun sequence.
ACCESSION   DATCBJ010000510 DATCBJ010000000
VERSION     DATCBJ010000510.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 4088)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4088)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4088
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_347757"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..195
                     /locus_tag="VII50_10360"
     CDS             <1..195
                     /locus_tag="VII50_10360"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HEY5051293.1"
                     /translation="GRRTVLLSRQFRYPVYVNDHPDGMLIETAAGLLDEDDPQTAIRR
                     EASEELGVTVGQLTHVSTCT"
     gene            192..419
                     /locus_tag="VII50_10365"
     CDS             192..419
                     /locus_tag="VII50_10365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5051294.1"
                     /translation="MSPGSVTERIHFFAAPYTDASRTSEGGGLADDGEDIDVLELPFD
                     EALAMVGAGEIADAKTIMLLQWAALSGPFRS"
     gene            complement(470..1093)
                     /locus_tag="VII50_10370"
     CDS             complement(470..1093)
                     /locus_tag="VII50_10370"
                     /EC_number="1.1.1.298"
                     /inference="COORDINATES: protein motif:HMM:NF003768.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonic semialdehyde reductase"
                     /protein_id="HEY5051295.1"
                     /translation="MTQTIETPTTALARLDDSGRAVLFIGARTANTFADTAVTDSELA
                     EIWNLAKWPPTAANVQPLRVLYVRTKAGRDRLAAHMSDNNKAKTAAAPSVAVLAFDTQ
                     YHEHIPTVLPFRPELKDVFAANDKMRNDTGRFSAALQAGYFLLAVRAQGLAAGPMGGF
                     DAEALDADFFPDGRWRSILVVNIGHPGENPWFDRLPRLDEADVVRWA"
     gene            1169..1507
                     /locus_tag="VII50_10375"
     CDS             1169..1507
                     /locus_tag="VII50_10375"
                     /inference="COORDINATES: protein motif:HMM:NF013777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEY5051296.1"
                     /translation="MPREQALDHEPRACDAALTRAFGFLGKRWNGVIIGTLALRPAGF
                     SELKRAVAGISDSVLSERLAELGGAGLVQRSVSEGPPIAVEYQLTNAGQALLPALQEL
                     TAWAKDNLPA"
     gene            1578..2096
                     /locus_tag="VII50_10380"
     CDS             1578..2096
                     /locus_tag="VII50_10380"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SigE family RNA polymerase sigma factor"
                     /protein_id="HEY5051297.1"
                     /translation="MTFEQFIATRLPGLLRHAVVLTGDRELAQDVVQEVLARAQVKWR
                     HIAKADSPDAYVRRMVVNEYLSWRRSWAVRNVRAVGERLDAMGDARATERDHAQTVVD
                     ADELWTRLATLPRKQRAVLVLRYYEQLDDIAIADVLDCAPATVRSNASKALKNLRLTS
                     EQQTRVHEKELT"
     gene            2093..3136
                     /locus_tag="VII50_10385"
     CDS             2093..3136
                     /locus_tag="VII50_10385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5051298.1"
                     /translation="MNQDADQLREAFVAHEYLAPDPAEVIAVVHERARRYRRRRRAAQ
                     GAGGAVLGAGLVASAVAAPGWLPNTRHGESSISLAAPTPQATPTALTSPSPLTAAQQE
                     EALNAYFDAGYDYNDALALAQLWHMSGDPTSIKAEAGRRLLAGQKLPFTPTPQAVASG
                     AAMADLNAFFAAGYTYEDAVGLAQLWKTSDPYQAKIDAGGKLVAGETLPIKPGSQPTP
                     PAASVTPSESVSATDLDAYFAAGYGYDDALQLAQLWHMTGDPATVKAEAGRRLLAGQK
                     LPITPMTPSPTPPGSAPVNGTDAAVAAYFSAGYTYNDAVTLGRMWNISDTYQVKADAG
                     QKLLAGQTLPIRP"
     gene            3133..>4088
                     /locus_tag="VII50_10390"
     CDS             3133..>4088
                     /locus_tag="VII50_10390"
                     /inference="COORDINATES: protein motif:HMM:NF023645.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidoligase family protein"
                     /protein_id="HEY5051299.1"
                     /translation="MTEVLHRRAGFEIELMAPVGSSRRVLAQELADRRGGRVRPVWHH
                     DSEPSLVPGLGRFLHLTQGFEVTDAAGAVVCTLVDDVTLIADLDQRTRPAPDWYRVLT
                     DDARLLRLLAECIDPAASIQESLDPVAALWGVHVDRHGDVFQVNDSTGATIALAAPQG
                     GGRERGCEVITPPLTAEHEAAINDLLRVARDLGFVVPTEAAVHVHHDGARFRSAAAIA
                     NVVRLFGLWRPALHSLLGTNPACRRLAPLPASLVHAAEGTPSYEELREAATAGGLTKF
                     FDVNLTALFAENPVRDTLEVRILPGAIDASDVVHGAAVVEALL"
BASE COUNT          699 a         1386 c         1356 g          647 t
ORIGIN      
        1 ggccgccgca cggtgctctt gtcgcggcag ttccgctatc cggtctacgt caacgaccac
       61 cccgacggca tgctgatcga gacggcggcc ggactcctcg acgaggatga tccgcagact
      121 gcgatcaggc gcgaggccag cgaggagctc ggcgttacgg tcggtcaact tacccacgtt
      181 tcgacgtgta catgagtccg ggatcggtta ccgaacggat ccactttttc gccgcgccct
      241 acaccgatgc gagccgcacg agcgaaggcg gcgggctcgc ggacgacggt gaagacatcg
      301 acgtgctcga actgcctttc gacgaggcgc tggcaatggt cggcgccggc gagatcgctg
      361 atgcgaagac catcatgctg ctgcagtggg cagcgctgag cggacctttc cgcagctagg
      421 ggcaggccgg tcagacgcag acccagctca gacgcaggtc agacctagct caggcccagc
      481 ggacgacgtc ggcctcgtcg aggcggggca gccggtcgaa ccaggggttc tcgcccgggt
      541 ggccaatgtt gacgaccagg atcgagcgcc agcgaccgtc tggaaagaag tcggcatcca
      601 gcgcttcggc gtcaaatcca cccatggggc cggccgcaag gccctgcgcg cgaacggcta
      661 acaggaagta gccagcctgc agcgcggcac tgaaacggcc cgtatcgtta cgcatcttgt
      721 cgttggccgc gaagacgtcc ttcagctcgg gccgaaacgg cagcactgtc ggaatgtgct
      781 cgtggtactg cgtgtcgaac gccagcacgg cgacggacgg agcagccgcg gtcttggcct
      841 tgttgttgtc cgacatgtgg gccgcgagtc ggtcgcgccc agccttcgtg cgaacgtaca
      901 ggacgcgcag cggctggaca ttggccgcag tcggcggcca cttcgcgagg ttccagatct
      961 ctgcgagctc gctgtcggtc acggccgtgt cggcgaaagt gttcgccgtc cgagcgccga
     1021 tgaagagaac cgcgcgaccg ctgtcatcaa ggcgggccaa cgcagtggtt ggggtttcta
     1081 tggtctgggt cattgtgctt ccgttctcag tgttactagg aaaaaagaag taactccatc
     1141 gaaggtagca caccggtacg atgtctgcat gccgagagag caagccctag atcacgagcc
     1201 gcgggcgtgc gacgcggcgc tgacgcgcgc gttcggcttc ctcggcaagc ggtggaacgg
     1261 ggtgatcatc ggcacgctgg cgctccggcc ggccggattc tcagagttga agcgggcggt
     1321 ggccggaatt agcgactccg tgctgtcgga gcggttagcc gagctgggcg gcgccggtct
     1381 ggtgcaacgc tcggtcagcg aaggaccgcc gatcgccgtc gagtaccagc tcacgaacgc
     1441 cggacaggcg ctcttgcccg cgcttcagga actcaccgcc tgggcgaagg acaacctgcc
     1501 cgcctgatca gcagactcgg gtgagaaagt cggcgtcgcc gctgcacgtt ccgggcatct
     1561 agcgcgtctg tagtggcgtg accttcgagc agttcattgc cacgcgactg cccggcctgt
     1621 tgcgtcacgc cgtcgtcctg accggcgatc gtgagctcgc ccaagacgtc gtccaggaag
     1681 tgctggccag ggcgcaggtc aagtggcggc acattgcgaa ggccgactcg cccgacgcgt
     1741 acgtgcgccg gatggtcgtg aacgaatacc tgtcgtggcg tcgcagctgg gcggtgcgca
     1801 acgtccgcgc cgtcggtgag cgcctcgacg cgatgggcga tgcccgcgcg acggaacgcg
     1861 accatgcgca gaccgtcgtc gacgccgacg aactctggac ccggctggcc acgctcccgc
     1921 gcaaacagcg tgcggtgctc gtgctgcgct attacgaaca actcgacgac atcgcgatcg
     1981 cggacgtact cgactgcgcg ccggcgaccg tgcggagcaa tgcatcgaaa gcattgaaga
     2041 acctgcggct cacctcggag cagcagacca gggtgcatga gaaggagctg acatgaacca
     2101 ggacgccgac cagttgcgcg aggcattcgt cgcgcacgag tacctcgctc ctgatcccgc
     2161 ggaggtcatc gcggtcgtgc acgagcgggc ccgcaggtat cgccggcgtc gccgcgccgc
     2221 gcagggggcc ggcggcgccg tactcggcgc cgggctggtt gccagtgccg tcgccgcgcc
     2281 ggggtggctg ccaaacaccc ggcacggcga atcctccatt tcgttggccg cccccacgcc
     2341 gcaggcgacc cccaccgcat tgacgagccc gtcgccactc actgctgcgc agcaagaaga
     2401 ggcgttgaac gcctacttcg acgccggcta tgactacaac gatgccctcg cactcgcgca
     2461 gctgtggcac atgtccggcg acccgacctc catcaaggct gaggccggtc gacgcctgct
     2521 ggccggtcag aagttgccat tcacgccgac cccacaagcc gtcgcgagtg gcgccgcgat
     2581 ggcggacctc aacgccttct tcgcggcggg ttacacctac gaggacgccg tcggacttgc
     2641 gcagctgtgg aagacgtccg acccctacca agcgaagatc gatgccggtg gcaagctcgt
     2701 cgccggcgaa acactgccta tcaagccagg ctcgcaaccg acgccgcccg cggcgagcgt
     2761 cactccgagc gagagcgtga gcgccaccga cctcgacgcc tacttcgcgg ccggttacgg
     2821 ctacgacgac gcgctgcagc tcgcgcagct gtggcacatg accggtgacc cggcgacggt
     2881 caaggctgag gccggacgcc gactgctcgc cggacagaaa ctgccgatca ccccgatgac
     2941 gccgtcgccg accccgcccg gttctgcgcc cgtaaacggc accgacgctg ccgtggccgc
     3001 ttacttctcg gcgggctaca cctacaacga cgcagtgacg ctcggcagga tgtggaacat
     3061 ctcggatacc taccaggtga aggccgacgc cggccagaag ctgcttgccg ggcagacgtt
     3121 gccgatcagg ccgtgaccga ggtgctccac cgccgcgcgg gcttcgagat cgagctgatg
     3181 gcacccgtcg gctcaagccg gcgggtgctc gcgcaggaac tggccgatcg ccgcggcggc
     3241 cgggtccgtc cggtatggca ccacgacagc gagccctcgc tcgtccccgg cctcggacga
     3301 ttcctgcatc tgacgcaagg tttcgaggtc accgacgctg ccggcgccgt tgtctgcacg
     3361 ctcgtcgacg acgtcacgct gattgccgat ctcgaccaac ggacacgacc agcaccggac
     3421 tggtaccgcg tgctgaccga cgacgcgcgg ctgctccggt tgcttgccga gtgcatcgat
     3481 ccggccgcct ctatccaaga gtcgttggac cccgttgctg cgctgtgggg cgttcacgtc
     3541 gaccggcacg gcgacgtgtt ccaagtcaac gactcgaccg gagcgaccat cgcgcttgcc
     3601 gcaccgcaag gcggagggcg tgaacgcggc tgcgaggtga tcacaccgcc attgacagcc
     3661 gagcatgagg cggcgattaa cgacctcctg cgggtagcgc gcgacctcgg cttcgtcgtg
     3721 cctaccgagg ccgcggtgca tgtgcaccac gacggcgccc ggttccgctc cgctgccgcc
     3781 atcgccaacg tcgtccggct atttgggttg tggcgaccgg cactgcactc cttgctcggc
     3841 accaacccgg cgtgccggcg gctggcgccg ctacctgcgt cgctcgttca cgcggccgag
     3901 ggcaccccgt cgtacgagga acttcgtgag gccgccaccg ctggcggcct cacgaagttc
     3961 tttgacgtca acctcactgc gctgttcgcc gagaacccag tacgcgacac cctcgaggta
     4021 cgaattctgc ccggtgccat cgatgcgtcg gacgtcgtgc acggtgccgc cgtcgtcgaa
     4081 gcgctgct
//
LOCUS       DATCBJ010000511         2404 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_12270, whole genome shotgun sequence.
ACCESSION   DATCBJ010000511 DATCBJ010000000
VERSION     DATCBJ010000511.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2404)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2404)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2404
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_12270"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            274..855
                     /locus_tag="VII50_10395"
     CDS             274..855
                     /locus_tag="VII50_10395"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEY5051300.1"
                     /translation="MLELSTSELLAQAAAGNSSAWDEIVSRYSGLVWAVARGFALSMA
                     DAADVSQTTWLRLVEHLGKLREPEHLGGWLATTARHECLRTLRKSGREVIAFDADIDI
                     ESGEPTPEAVVLHDEQDRLVWLSLGEIPQRCQVLLRALSSTPPPSYDEVSAALGMPIG
                     SIGPTRARCLDHLKKRLKAHQVDTGRRMREGGT"
     gene            855..1307
                     /locus_tag="VII50_10400"
     CDS             855..1307
                     /locus_tag="VII50_10400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5051301.1"
                     /translation="MAEPSTDPMFATLRSVIGRADPVPAAVVDAARAAYTWRTIDGEL
                     AELTADSAMSTAGVRSTSAPRLLTFEGAGVEVEVEVAQTGSTRHLSGQLVPVGPAQIT
                     VRWSGGTQESTADDLGRFAVDGVPAGSVSLAIVRAGADQPIVTSWISI"
     gene            1327..>2404
                     /locus_tag="VII50_10405"
     CDS             1327..>2404
                     /locus_tag="VII50_10405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5051302.1"
                     /translation="MTNALELTQLQQRAEAVYTQVPVAPARAEGIARRLIDEAGSDPT
                     AQVMAMRALALTVRLSAGPNESIKILREAVRLGERHRLGRCLAEARMTYAALLADTGR
                     IPTALAECDRAAEVLKGKDAGPLLAQRALILARAGRSEEAMSNFARALPLLRAANDVQ
                     FQCLLFINRSNLLAYLGRLAAAERDLQRGIALARAHGLHDRVVVLSANLGFLKVQGGD
                     IPAALRLFAEVLEGGSKAPGLVATHDRAEALLMAGMPGEARESLVRRVDDVERAGFAV
                     DLAEWHLLLAQAALMEGEAEVARTSAQRALTEFRAQGRARWALLAQHLVIRARWASGE
                     RTPALIRAARDAHAKLWTAGWQVAA"
BASE COUNT          395 a          813 c          829 g          367 t
ORIGIN      
        1 tcacgggcta cgcgacatgg agcggcacct ctttcgccgc gccgagggtt gcggcagcgg
       61 tcgcagcacg cgccgcagcg cgcggggtgg ccgtggccgt cgccgcggcc gagctgctcg
      121 acccgagcga tcatccctcc acgcccgatc ttggcgtgct ggtcgagccg tgacccgaag
      181 tgactccgga gccgtcacca tgagcgctat cgtcgtgaac ctgtgcaacc cgcgacccgc
      241 gggccgaacc gaccccatcg ggagggccct gccgtgctcg agctcagcac cagcgagctc
      301 ttggcacagg ccgcagcggg caacagctca gcctgggacg agatcgtcag ccgctactcc
      361 ggtctggtct gggctgtcgc acgcgggttc gcactctcca tggctgatgc cgccgacgtc
      421 agccagacga cctggttgcg gctcgtcgaa catctcggca agctgcgcga gcccgagcac
      481 ctcggcggct ggctggccac gacggcacgc cacgagtgcc tgcgcacact gcgcaagagc
      541 ggccgcgagg ttatcgcctt cgatgccgac atcgacatcg agtcgggcga gccgaccccc
      601 gaagcagtgg ttctccacga cgagcaggat cggctcgtct ggttgtcgtt gggcgagatc
      661 ccgcagcgct gccaggtgct gctgcgcgcc ctgtccagca cgccgccgcc gagctacgac
      721 gaggtgtctg cagctctggg gatgcccatc ggcagcattg gccccacccg ggcacggtgt
      781 ctcgaccatc tcaagaagcg cctcaaggcc caccaagtcg atacgggccg ccgcatgcgg
      841 gaaggaggca cgtgatggcc gagcccagca ccgacccgat gttcgccacg ctccgtagcg
      901 tgatcggccg tgccgaccct gtgcccgcgg cggtagtcga cgccgcacgg gccgcataca
      961 cctggcggac catcgacggc gagctcgctg aactcaccgc ggactccgcg atgtccacgg
     1021 cgggcgtgcg gtcaacgtct gcacccaggc tgctgacctt tgaaggcgcc ggcgtcgagg
     1081 tcgaggtcga ggtggcgcaa acggggtcga cacggcacct gtccgggcag ctggtgcccg
     1141 ttgggcccgc gcaaatcacg gtgcgctggt cgggcgggac gcaagaatcg accgctgacg
     1201 acctcggtcg attcgcggtc gacggcgtac ctgccggcag cgtgagcctc gccatcgtca
     1261 gagcgggcgc cgaccagccg atcgtgacga gctggatctc gatctgacac gctgcgcaac
     1321 atgtcggtga ccaacgcact ggagctgacg cagctgcagc agcgggctga agcggtctac
     1381 acccaagtgc cggttgcacc agcgcgcgcc gaaggaatcg cgcgacggtt gatcgacgag
     1441 gcggggtcag atccgaccgc gcaggtaatg gcgatgcgcg cgttggctct gacggtgcgt
     1501 ctgtctgccg ggccgaacga atcaatcaag atcttgcgcg aggccgttcg tctcggtgag
     1561 cgacatcgcc tcggcaggtg cttagccgaa gcacgaatga cgtacgccgc gctgctcgcc
     1621 gacaccggtc ggataccaac tgcactcgcc gagtgcgatc gggctgcgga agtcttgaaa
     1681 ggcaaggatg cggggccgtt gctggcccag cgcgcgctga tactcgctcg ggccggtcgg
     1741 tccgaggagg cgatgtcgaa cttcgcccgc gcactcccgc tgttgcgagc ggcgaacgac
     1801 gtccagttcc aatgcctgct cttcatcaac cgcagcaatc tgctcgctta cctcggacgg
     1861 ctggcagcgg ccgagcgcga tctgcagcgc ggcatcgcgc tagcgcgcgc acatggtctg
     1921 cacgaccggg tcgtcgtcct gtcggcgaac ctcggctttc tcaaagtcca aggcggcgac
     1981 attcctgcag ccctccgcct gttcgccgaa gtgctcgagg gaggaagcaa ggcgccaggg
     2041 ctggtcgcaa cgcatgatcg agccgaagcg ctgctcatgg ccggcatgcc cggcgaggca
     2101 cgagagtcac tcgttcgacg cgtcgacgac gtcgaacgcg ccgggttcgc ggtcgacctc
     2161 gcagagtggc acctcttgct cgcgcaagct gcgctgatgg aaggcgaagc ggaggttgcg
     2221 cgcacatcgg cgcaacgagc gctcaccgag tttcgagcac agggccgggc ccgctgggca
     2281 ctccttgcgc agcatttggt gatccgcgct cggtgggcaa gcggtgaacg gacgccggcg
     2341 ctcatccggg cagcacgcga tgcgcacgcg aaattgtgga cggcggggtg gcaggtcgcg
     2401 gcgt
//
LOCUS       DATCBJ010000512         2376 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_330635, whole genome shotgun sequence.
ACCESSION   DATCBJ010000512 DATCBJ010000000
VERSION     DATCBJ010000512.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2376)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2376)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2376
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_330635"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..208
                     /locus_tag="VII50_10410"
     CDS             <1..208
                     /locus_tag="VII50_10410"
                     /inference="COORDINATES: protein motif:HMM:NF023917.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3710 domain-containing protein"
                     /protein_id="HEY5051303.1"
                     /translation="FRSLETVLTSIVVVRGTDPMAPRDPLQLRLPREATAAREAAEAA
                     EGDGAKPSPVFELPERGREITETS"
     gene            225..665
                     /locus_tag="VII50_10415"
     CDS             225..665
                     /locus_tag="VII50_10415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold nucleic acid binding domain-containing
                     protein"
                     /protein_id="HEY5051304.1"
                     /translation="MKESEVVAAHDSAGTAAAADETAGSPGVLRRALHRLTTPEHVLE
                     AESRQEQSLESGGTPVHRCRDRAQVCVVGMLRTVTIQPRGGSPSLQAELWDGSGSVDV
                     VWIGRRRIAGIEPGRVIRAEGRMTQRDGRRVMFNPRYELRPSGS"
     gene            complement(675..1337)
                     /locus_tag="VII50_10420"
     CDS             complement(675..1337)
                     /locus_tag="VII50_10420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020671877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HEY5051305.1"
                     /translation="MRVAIAGAGAVGRSIAAELVENGHEVLVIDKDSRAIDVDAIPGA
                     EWVLADACEIANLDELGLDRCQVAVAATGDDKVNLVVSLLAKTEYGVPRVVARVNDPV
                     NEWLFDESWGVDVAVSTPRLLSALVEEAVSVGDLVRLMTFRQGQANLVEFTLPPTGRY
                     VGQRVGSVPWPADSALVVILRDGRVIVPSSDDPLEGGDELLFVASPDVEDELRGLLLH
                     GE"
     gene            complement(1337..2002)
                     /locus_tag="VII50_10425"
     CDS             complement(1337..2002)
                     /locus_tag="VII50_10425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HEY5051306.1"
                     /translation="MHVVIMGCGRVGSTLADSLEELGHSVAIIDIDAHAFRRLPPHFR
                     GRKIHGVGLHRTVLEESGIRQAGAFAAVSSGDNSNIIAARVARETFGVENVVARIYDI
                     RRAEIYERLGIPTVATVKWTADQMLRRVLQEGAQALWRDPTGTVQLAEVHVASGWIGR
                     RLSEIETAAGVRVAFVTRFGEGVLPTPDTVFQEGDLFNIIVRDKFADKAIAVLGAAPE
                     ENG"
     gene            2090..>2376
                     /locus_tag="VII50_10430"
     CDS             2090..>2376
                     /locus_tag="VII50_10430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HEY5051307.1"
                     /translation="MSIASGVFKRVLVGRPQRSDRLGESLLPKRLALPVFASDALSSV
                     AYATEQILLVLSAGGLALLHYTWYAAAGVAFLMLVVVASYRQNVHAYPSGGG"
BASE COUNT          372 a          804 c          805 g          395 t
ORIGIN      
        1 cttccgatct ctcgaaacgg ttctgacgag catcgtggtg gtccgcggta ctgatccgat
       61 ggcgccgcgt gacccgctgc agttgcggct gccgcgcgaa gcaaccgcgg cgcgtgaggc
      121 ggcggaggcg gcagagggcg acggcgcaaa gccctcacca gttttcgagt tgcccgagcg
      181 cggccgggag atcaccgaga cgagctgatg ggtatccttt atccatgaag gagtccgagg
      241 tcgtcgcggc gcacgactca gccggtaccg ccgcggccgc cgacgagacc gccgggtcgc
      301 ctggcgtgct gcgcagggcg ctgcaccggc tgacgacccc ggagcatgtc ctcgaagccg
      361 agagccgcca ggagcagtcg ctcgaaagcg gcggcacccc cgtccaccgc tgccgcgacc
      421 gggcacaggt ctgtgtcgtc ggcatgctgc gtacggtgac gatccagccg cgcggcggat
      481 cgccgtccct gcaggccgaa ctgtgggacg gctctggctc ggttgacgtc gtgtggatcg
      541 gcaggcgtcg catcgcgggt atcgaacccg gccgggtgat ccgggctgaa ggccggatga
      601 cccaacgcga cggtcgtcgg gtgatgttca accccaggta cgagctgcgt ccgtcaggct
      661 cgtaacgcgc agacttactc gccgtgcagc agtagcccgc gcaactcgtc ttcgacgtcc
      721 ggcgaggcca cgaacaacaa ctcgtcgccg ccttcgagcg gatcgtcgct gctcggcacg
      781 atcactcgac cgtcgcgcag gatgaccacc agggcgctgt ctgcaggcca cggcaccgaa
      841 cccacccgct gcccgacgta gcggccggtg gggggcaggg tgaactcgac cagattggcc
      901 tggccttgcc ggaaggtcat cagccgaacc aggtcgccga cgctgactgc ttcttcgaca
      961 agtgcggaaa gcagccgcgg cgtcgacacc gcgacgtcga cgccccagga ctcgtcgaac
     1021 aaccactcgt tgaccgggtc attcacccgg gccacgaccc tggggacgcc gtactccgtc
     1081 ttcgcgagca gcgagacgac gaggttcacc ttgtcgtcgc cggttgccgc gacggcaact
     1141 tggcagcggt cgagcccaag ctcgtcgagg ttggcaatct cacaggcgtc cgcgagcacc
     1201 cactcggcac ccgggatggc gtcgacgtcg attgcccggc tgtccttgtc gataacgagg
     1261 acttcgtgac cgttctcgac caattcggcg gcaatcgatc ggccgacggc accggctccg
     1321 gcaatggcga ctcgcatcag ccgttctcct ccggcgccgc tccgagcacc gcgatcgcct
     1381 tgtcggcgaa cttgtcgcgc acgatgatgt tgaacagatc gccctcctga aacacggtgt
     1441 cgggggtcgg caggacgcct tcgccgaagc gggtcacgaa ggcgacgcgg acgccggcgg
     1501 cagtctcgat ctcggagagt cgccgcccga tccagccgct ggcgacatgc acctcggcca
     1561 actggaccgt gcccgtcggg tcgcgccaca gcgcctgggc gccctcttga aggacccggc
     1621 gcagcatctg gtcggcggtc cacttcacgg tcgcgaccgt ggggatgccg aggcgttcgt
     1681 agatctctgc tcggcggatg tcgtagatcc gggcgaccac gttctcgacg ccgaaggtct
     1741 cgcgggcgac ccgcgccgcg atgatgttgg agttgtcacc gctgcttact gcagcgaagg
     1801 cccccgcctg acggatgccg gactcctcga gcaccgtgcg gtgcaggccc acgccgtgga
     1861 tcttgcggcc acggaaatgg ggcggcagcc gccgaaacgc gtgtgcgtcg atgtcgatga
     1921 tcgcgacgga gtgcccgagt tcctcgaggc tgtccgccaa ggtcgacccg acccggccgc
     1981 atcccatgat cacgacatgc acggttggct tgtctccttc agtgtgcctg gggaacgcta
     2041 cacggtgcgg ccgccagggc ctgggacggc tatcgcctag catcttgacg tgtccatcgc
     2101 cagcggtgtg ttcaaacggg tgctggtcgg tcggccacaa cgcagtgacc ggctggggga
     2161 gtcgctactc cctaagcggc tggcgcttcc ggtgtttgcg agcgacgcct tgtcgtcggt
     2221 cgcttacgcc accgagcaga tcctcctcgt gttgtcggcc ggcggcttgg ctctcctgca
     2281 ctacacctgg tacgcagccg cgggcgtcgc gttcctcatg ctggtggtcg tcgcgtcgta
     2341 tcggcagaac gtgcatgcgt atccgagcgg cggcgg
//
LOCUS       DATCBJ010000513         2921 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_418483, whole genome shotgun sequence.
ACCESSION   DATCBJ010000513 DATCBJ010000000
VERSION     DATCBJ010000513.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2921)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2921)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2921
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_418483"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            29..2287
                     /locus_tag="VII50_10435"
     CDS             29..2287
                     /locus_tag="VII50_10435"
                     /inference="COORDINATES: protein motif:HMM:NF037660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HEY5051308.1"
                     /translation="MPEPEVLRILARAEGNPFFIEELVAAATVGGALPADLADLLLVR
                     LEQIGGEGRLVVRAASVAGRRVSHELLARGAELDNTALDTAVRAAVEANILVPLGDDG
                     YGFRHALLAEAVYQDLLPGERVRLHAAYAAALASHQVQGSAAELSRHARASHDLITAT
                     RASIEAGDEAMTVGGPEDALHHYELALELLGDPHVAAGVAADPDAPNRVALVVRASMA
                     AAAAGHPYRAIALSEDELAALPADAPKLSRVRLIHAIVSTALILDTNLDLLGMTTEAV
                     QLLANEPPSSIRAHILNVHARANADRSRDDEAIRWATEALAMAQSLQLAEVATDATLL
                     LARLDERAGNPDAAKAAMTKAIAEAAAAGEQMAELRGLYNLASLDYGQGRLSQALALF
                     QQAATKARDKGRPWAQYGLEATVIGAIVAYVAGDWDLAEQMIDSSGQNPPEQARVMLD
                     AVAIEIAAGRGDLSWLEALPRLRNWWLRDGLIGITSGGAAIELYGYLGDIDAATAAYD
                     EVVASVARVWQYMEFGARVRLAALLLGQLANAASTATAAEREQLVRRGDQLARTALKV
                     AASVRHNGPEGRAWVSRVAAEKARLHWLGGGADSTPEAGLIEASQESVDAFAAFGHVY
                     ETARSRVRLAAVLRAAGKVAEAKVEFEAARKVAERLGAEPLLRELRGLPGYDVGAVRH
                     VVKRESQALTPREQEVLTLVATGRSNRDIAGQLFISAKTVSVHISNMLAKLEATSRTE
                     AVAVARRRGLLG"
     gene            complement(2350..>2921)
                     /locus_tag="VII50_10440"
     CDS             complement(2350..>2921)
                     /locus_tag="VII50_10440"
                     /inference="COORDINATES: protein motif:HMM:NF044584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="malate synthase A"
                     /protein_id="HEY5051309.1"
                     /translation="RRDQAVNEVALAKVRDDKTREAGDGFDGSWVAHPDLVPLCSEVF
                     TAVLGQRPDQRERLRDDVRANAAQILNAHATPGAMTEEGLRNNISVAVQYLASWLRGG
                     GAVAINNMMEDAATAEICRTQVWQWLHNDVMLSNGRPVTRRVVSWMLNEELRTLGPDY
                     AAGRDLFEEVTLTDKYVDFLTLPAYERMP"
BASE COUNT          453 a          994 c          998 g          476 t
ORIGIN      
        1 gcttatccgg gcactgcaca gccgacctat gcctgagccc gaggtcctgc gcatccttgc
       61 ccgcgccgag ggcaacccat tcttcatcga ggagctagtc gcggcggcaa ccgtcggcgg
      121 tgcgctaccg gcggatctcg ccgacctgct tcttgtgcgc cttgagcaga tcggcggcga
      181 gggccggctc gtcgtccgcg ctgcgtccgt tgcgggccgc cgcgtctcgc acgaactgct
      241 cgcgcgcggc gccgagctcg acaacactgc gctcgacacc gcggtgcgcg ccgcggtcga
      301 ggccaacatc ctggtcccgc tcggcgatga cggttacggc ttccgccacg cgttgttagc
      361 cgaggcggtc taccaggatc tgctgcccgg cgagcgagtc cggttgcatg cggcgtatgc
      421 cgctgccctc gcgagccatc aagttcaagg gtcggcagcc gagctgtccc gccacgctcg
      481 cgcctcgcat gacctcatca ccgccacccg cgcgagcatc gaggcgggcg acgaggcgat
      541 gacagtcggc ggccccgaag atgcgctgca ccactacgag ctcgcgctcg agcttctcgg
      601 tgacccgcac gtcgcggccg gggttgccgc tgatccggat gcgcccaaca gggtggcgct
      661 tgtcgtgcgg gccagcatgg ccgccgccgc tgctggccat ccgtaccgtg cgatagcgct
      721 gtccgaggac gagctggctg ccctgcctgc cgacgcaccg aagctctccc gtgttcggtt
      781 gatccacgcg atcgtgtcaa ccgcgctgat ccttgacacg aacctcgacc tgctcggcat
      841 gacgaccgag gccgtgcaac tcctggccaa cgagccaccg agctcgatac gtgcgcacat
      901 cctcaacgtc cacgctcgcg ccaacgccga ccggtcgcgg gatgacgaag cgatccggtg
      961 ggctaccgag gcgctggcaa tggcccagtc gctgcaactc gccgaggtcg cgaccgatgc
     1021 gacgttgctg ctggcgcgtc tcgacgaacg cgcgggaaat ccggacgcgg caaaagctgc
     1081 gatgaccaag gccatcgccg aggctgccgc tgcgggcgag cagatggccg agcttcgcgg
     1141 cctgtacaac ctggcgagcc tcgactacgg ccagggccgg ctcagccagg cactggcgct
     1201 tttccaacag gctgcgacca aggcccgaga caagggccgg ccgtgggcgc agtacggcct
     1261 cgaggccacc gtgatcggcg cgatagtcgc ctacgttgcg ggcgattggg accttgccga
     1321 acagatgatc gactcctccg ggcagaaccc acccgagcag gcacgagtca tgctcgacgc
     1381 tgtcgcaatt gagatcgccg ccggtcgcgg tgacctcagc tggctcgaag ccttgccgcg
     1441 gcttcggaat tggtggctgc gcgacgggtt gattgggatc acgagcggcg gagccgcgat
     1501 cgagttgtac ggctacctcg gcgacatcga cgctgcgact gccgcgtacg acgaagtcgt
     1561 cgccagcgtc gcccgagtct ggcagtacat ggaattcggg gcccgcgtcc ggctggcggc
     1621 cttgctgctc ggccaactcg caaacgcagc gtccacggca acggccgcgg agcgtgagca
     1681 gttggtgcgc cgcggcgatc agctcgcgag aacggcgctg aaggtcgcgg catcggtccg
     1741 tcacaacggt cctgaaggcc gggcatgggt cagccgcgtc gccgccgaga aggcccgtct
     1801 gcactggctg ggcggcggtg ccgactccac acccgaggcc ggactaatcg aagcctcgca
     1861 ggagtcggtc gacgcgttcg ctgcattcgg tcacgtgtac gagacggcac gatcgagggt
     1921 tcggttagcc gcggtgctgc gtgcggcagg caaggtcgcg gaggcgaagg tcgagttcga
     1981 ggcggcacgg aaggtcgccg aacgcctcgg tgccgagccg ctattgcgcg agctgcgcgg
     2041 gctgcccggc tacgacgttg gtgcggtgcg ccatgtcgtc aagcgagagt cacaggccct
     2101 cacgccgcgc gagcaggaag tgctcacctt ggtcgcgacc ggccgcagca accgcgatat
     2161 cgcggggcaa ctattcatca gcgcaaagac cgtcagcgtc cacatctcca acatgctcgc
     2221 caagctcgag gcaacgagtc gcaccgaagc ggtggcggtc gcgcgccgcc gaggcctcct
     2281 cggctaggca tcgattccga tgccgccgcc ctggttgcga accgtcgctg caccaccgag
     2341 gcggtagttt cacggcatcc gttcgtaggc gggcagtgtc aggaagtcga cgtatttgtc
     2401 ggtgagcgtg acttcttcga acaggtcgcg cccggccgcg tagtcgggcc cgagggtgcg
     2461 cagttcctcg ttgagcatcc aggacacgac tctgcgggtg accggtctgc cgttgctgag
     2521 catgacgtcg ttgtgtagcc attgccagac ctgggtgcgg cagatttcgg cggtggcggc
     2581 gtcttccatc atgttgttga tcgcgactgc gccgccgcct cgcagccagg aggcgaggta
     2641 ctggacggcg acgctgatgt tgttgcgaag cccttcctcg gtcatcgccc caggcgttgc
     2701 gtgggcgttc aggatctgcg ccgcgttagc ccgcacatcg tcgcgcagcc gttcccgctg
     2761 gtcgggtcgc tgccccaaca cggccgtgaa cacctccgag cacagcggga ccaggtcggg
     2821 gtgggcgacc catgagccgt cgaacccgtc gcccgcctcg cgggtcttgt cgtcgcgcac
     2881 cttggcaagc gcgacctcgt tgaccgcctg gtcgcgcctg g
//
LOCUS       DATCBJ010000514         2496 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_322048, whole genome shotgun sequence.
ACCESSION   DATCBJ010000514 DATCBJ010000000
VERSION     DATCBJ010000514.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2496)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2496)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2496
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_322048"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..702
                     /locus_tag="VII50_10445"
     CDS             <1..702
                     /locus_tag="VII50_10445"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: protein motif:HMM:NF014156.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HEY5051310.1"
                     /translation="ELHPGLEIESCSSGGARIDFGVLEHTDRVWASDCIDPYERVRIV
                     SGLSTLLPFELIGAHVASGRSHTTGRTHELSFRLAVALFGHAGLEWDLTTTSPEVREQ
                     LATWVAFAKSVRQLLRTGEVVRVGRPADHETVLYGVVGPRRDEALFSLVRLQTGPRYG
                     TAPVVFAGLDPDLRYRVQRVSMPGEGPPVHGPAMMHDGVRDIVVPGSLLMGAGVAAPN
                     LQPEQAAIYHLRADS"
     gene            724..1488
                     /locus_tag="VII50_10450"
     CDS             724..1488
                     /locus_tag="VII50_10450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5051311.1"
                     /translation="MSGGALFLAVFLACTVEAVEATTIVLAAGTARDWRSALYGLFAA
                     LVTLAVLIGALGPALSAVPLHALRLVVGGLLLVFGLQWLRKAILRASGFKALHDEQQI
                     YAAEIAAAQAVPRERRGAVTDWYAFTLAFKGVVLEGLEVVFIALTFGANDHNLPLAAL
                     AAGVAVIVVALLGIAVRAPLARVPENSMKFVVGVMLTAFGTFWGAEGAGAHWPGSDAA
                     LPVVVGAVAVFAVVLVAALRRGRTSVQAVASLMVNP"
     gene            1485..1700
                     /locus_tag="VII50_10455"
     CDS             1485..1700
                     /locus_tag="VII50_10455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5051312.1"
                     /translation="MITRLRQFCAFWYDFVIGDDWHVAVGVVLALGVTYGMHRASLSA
                     WWVVPAAVAVLLPYSLWRASRSKPPKS"
     gene            1872..>2496
                     /locus_tag="VII50_10460"
     CDS             1872..>2496
                     /locus_tag="VII50_10460"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011718977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004024 - alcohol dehydrogenase activity,
                     zinc-dependent [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HEY5051313.1"
                     /translation="MEAWVVDHPAPIDAGPLRRIERDSAEPGAGQVRVRVSCCGVCRT
                     DLHLAEGDLSPRRIAVTPGHEIVGYIDALGAGADRFAIGDRVGVAWLASTDGSCRFCR
                     RGDENLCKASTFTGWDVDGGYADSCVADERFVYVLPSEISDEQAAPLLCAGIIGYRSL
                     LAADVPPGGRLGIYGFGGSAHLTAQIALHLGMRVHVLTRGSHNQELAR"
BASE COUNT          328 a          780 c          884 g          504 t
ORIGIN      
        1 gaactgcacc cgggccttga gatcgagtcg tgttcatccg gtggcgcgcg catcgacttc
       61 ggcgtgctcg agcacaccga cagggtttgg gccagcgatt gcatcgatcc gtacgagcga
      121 gttcggatcg tgtccgggct ctcgacgctg ttgccgttcg agctgatcgg tgcgcacgtg
      181 gcgtccggcc gcagccacac gacgggtcgc acgcacgagc tgtcctttcg acttgccgtg
      241 gcgctgttcg gccatgccgg gctcgagtgg gatctgacga ccacctcccc ggaggtacgc
      301 gaacagctcg ctacctgggt cgcgttcgcg aagtcggtac ggcagttgtt gcgcaccggc
      361 gaggtcgtgc gcgtcggtcg gcccgccgac cacgagacgg tgctgtacgg agtcgtcggt
      421 cctcggcgtg acgaggccct gttcagcctc gttcgactgc aaacgggccc gcgctacggc
      481 accgcgccgg tcgtgttcgc cggccttgat cccgatcttc gctaccgcgt gcaacgggtg
      541 tcgatgccgg gcgagggccc tccggtgcac gggccggcca tgatgcacga cggcgtgcgc
      601 gacatcgtcg tcccaggatc actgctgatg ggagcgggcg tggcggcgcc caacctccag
      661 ccagagcagg ccgcgatcta ccacctgcgg gcggacagct aacgttttcc tcgtgacgag
      721 cgcatgagcg gcggggcact gttcctcgcg gtcttcctcg cctgcacggt cgaggcggtc
      781 gaggccacga ccatcgtctt ggccgcgggt accgcgcgcg actggcggtc ggcgctctac
      841 ggcctctttg ccgcgctggt cacgctcgcc gtgctcatcg gcgcgctcgg accggcactg
      901 tccgccgtcc cgctgcatgc tctgcggctt gtggtcggtg gactcctgct cgtgttcggc
      961 ctgcaatggc tgcgcaaggc gatcctgcgg gccagcggtt tcaaggcgtt gcacgacgag
     1021 cagcagatct acgccgccga gatcgcagcc gcccaggctg taccccgtga acgtcgtggc
     1081 gctgtcaccg actggtacgc cttcaccttg gcgttcaagg gcgtcgttct cgagggcctc
     1141 gaggtggtgt tcatcgcgct gacgttcggt gccaacgacc acaatttgcc gttggctgca
     1201 ctcgcggccg gggtcgcggt gatcgtcgtg gcgcttttgg gaatcgctgt tcgtgctccg
     1261 ctggcacgag tgccggagaa ctcgatgaag ttcgtggtcg gcgtcatgct cacggcgttc
     1321 ggcacgttct ggggcgcgga aggcgcgggc gcgcactggc cgggatccga tgctgcattg
     1381 ccggtcgtgg tcggcgccgt cgcggtgttc gctgtcgtgc tggtcgcggc gttgcgtcga
     1441 ggtcggacgt cggtgcaagc ggttgcgagt ttgatggtga acccgtgatc actcggctgc
     1501 gacaattctg tgcgttctgg tacgacttcg tcattggtga cgattggcac gttgcggtcg
     1561 gcgtcgtcct ggcgctcggg gtgacgtacg ggatgcaccg tgcgtccctg tcggcgtggt
     1621 gggtcgttcc tgcggccgtg gcggttttgc tgccctacag cttgtggcga gccagcaggt
     1681 cgaagccgcc aaaatcttga ggtgaatgcg cgagccggat gtggcgcgcg gcgcaaaagg
     1741 agtgcattct tttggcgcag cgcccggcct gttgtgagtt tcccccccgg aggcagaccg
     1801 ggcgccgcat cgtctgacgg ttccctgctg gcgctgggtt aacccatggc atcggctggg
     1861 atgatcgagc cgtggaagct tgggttgtcg accaccccgc gccgattgac gccggacccc
     1921 tgcgccggat cgagcgcgat tctgctgagc ctggtgcggg acaggtccga gttcgggtga
     1981 gttgctgcgg cgtgtgccgc accgatctgc atctggccga aggcgatctg agcccacggc
     2041 gaattgcggt cactccaggt cacgagatcg tcggctacat cgatgcactc ggtgccgggg
     2101 cggatcggtt cgccatcggc gaccgcgtcg gcgtcgcgtg gctcgcgagt accgacggca
     2161 gttgccggtt ttgtcggcgt ggcgatgaga acctttgcaa ggcttcaaca tttactggct
     2221 gggatgtcga cggcggctac gccgattcgt gcgttgcgga tgagcgtttc gtgtatgtct
     2281 tgccatcgga gatcagtgac gagcaggcgg ctccgctgct gtgtgcgggc atcatcggct
     2341 accgctcgct cctcgcggct gatgtgccgc ccggcggacg cctgggcatc tacggctttg
     2401 gcggcagcgc gcacctgaca gcgcagatcg ctttgcatct cggcatgcgc gtacacgtac
     2461 tgacccgcgg atcgcacaat caggagctgg cccgga
//
LOCUS       DATCBJ010000515         3125 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_125630, whole genome shotgun sequence.
ACCESSION   DATCBJ010000515 DATCBJ010000000
VERSION     DATCBJ010000515.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 3125)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3125)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3125
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_125630"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1714
                     /locus_tag="VII50_10465"
     CDS             <1..1714
                     /locus_tag="VII50_10465"
                     /inference="COORDINATES: protein motif:HMM:NF013225.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HEY5051314.1"
                     /translation="DDYTLKVLRSRARGTIYPYELVPLLTGPDGTFVEHDLDDGGRLV
                     PVERPPGRNGAGIVAGVVSTPTARYPEGMTRVALFGDPTKALGTVAEPECALVVAALD
                     LAEQRGIPVEWFALSSGARISMDSGTENMDWVARALRRLITFTQGGGEVNVIVAGINV
                     GAQPYWNAEATMLMHTKGILVMTPDSAMVLTGKHSLDYSGGVSAEDNFGIGGYDRVMG
                     PNGQAQYWAPNLTAACDLLFAHYEHTYIAPGERWGRAAHTNDPRERDVCSFPHTHPSS
                     DFRTVGDIFSPVMNPARKKPFDIRTVMHAVVDQDHPVLERWAGMHDAETVVVYDAHLG
                     GHPVTALGIESRPIQRRGSYRSDGPDHWTAGTLFPRSSKKTARAINACSGNRPLVVLA
                     NLSGFDGSPESLANMQLEYGAEIGRAMVNFVGPVVFCVISRYHGGAFVVFSGVLNDNI
                     EVIAIEGSFASVIGGAPAAAVVFSSEVNRRTAADPRVRDLETALTTADDVERRSLRDE
                     LAAARTAVRSAKLGEVAAEFEHVHNIERALQVGSVHAIIAARRLRPHLIDAVERGMER
                     SVGR"
     gene            1765..>3125
                     /locus_tag="VII50_10470"
     CDS             1765..>3125
                     /locus_tag="VII50_10470"
                     /inference="COORDINATES: protein motif:HMM:NF020134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5051315.1"
                     /translation="MQAFVLNRHGQMVFPSNLMPELDFSALESLEQLDRVIRRDFETK
                     APTGTDIMNHLENGTYESRYALLRDIALNLFWVNRFAMTMYDKRPTRWGDVPRTRSDV
                     FLPALTPWEDGDRKVAAVRAAYPTLPPRWDVAVEDRIFDVLFDVYGHRKHHGTTLPAI
                     KPTVAECLANPSTLTFRLPKYDPDYPVYEFSAIVDCYEDVPELQALHRWAMVLHNQYP
                     WDRAQVELTEVGQLKDDDYVVLFYPRDKEVRGFLRRLQRVATAPATPAAAEAEAKRPV
                     RPYAAIDIRRQFTVMPRIEALAVVHGDQICSNEDLIRNAAYNWSPMGADEIREKTGIE
                     QRRYTSLTMEELALRAAQAALAKAQRGPEEIGAVLVCTCTSSRLIPSVATYISGQLGI
                     YQTHASCDIVAACAGLPYGLAEATRLLQEVERPVLLVCVEKFSDKIGNVRPSRMIFGD
                     GAGA"
BASE COUNT          542 a         1015 c         1029 g          539 t
ORIGIN      
        1 cgacgactac acgttgaaag tgctgcgctc gcgcgcccgc ggcacgatct acccgtatga
       61 gttggtcccg cttctcacgg gccccgacgg cacgttcgtc gagcacgatc tggacgacgg
      121 cgggcggctc gtgcctgtcg agcgaccgcc gggtcgcaac ggtgcgggga tcgtggccgg
      181 cgtggtgagc acgccgacgg cgcgttaccc ggagggcatg accagggtgg ctctcttcgg
      241 cgatccgacc aaggcactcg gtacggtcgc cgagcctgag tgtgcacttg tcgtcgccgc
      301 cctcgacctc gcggagcagc gcggtatccc tgtcgagtgg ttcgcgctct cgtcgggggc
      361 caggatctcg atggacagcg gtaccgagaa catggactgg gtggcgcgcg cgctgcggcg
      421 gctgatcaca ttcacgcagg gcggcggcga ggtcaacgtc atcgtggccg gcatcaatgt
      481 gggtgcgcag ccgtactgga atgccgaggc caccatgctg atgcacacca aaggcatcct
      541 cgtcatgacg ccagacagcg caatggtgct caccgggaag cactcactcg actactcagg
      601 cggcgtgtcc gcggaggaca acttcgggat cggcggctac gaccgggtca tggggccgaa
      661 cggccaagcc cagtactggg cgcccaatct caccgccgcc tgcgatctgc tcttcgctca
      721 ttacgagcac acgtacatcg cgcccggcga acgctggggg agggcggccc acacaaacga
      781 ccctcgcgag cgtgacgtgt gcagcttccc gcacacgcac ccgtcgagcg atttcaggac
      841 cgtcggcgac atcttctcgc ctgtgatgaa tcccgcgcgc aagaaaccgt tcgacatccg
      901 cacggtcatg catgccgtcg tcgatcagga tcacccggtg ctggagcggt gggcaggcat
      961 gcatgacgcc gagaccgtcg tcgtgtacga cgcacatctc ggtggtcacc ctgtcacggc
     1021 gctgggcatc gagtctcggc cgattcagcg acgcgggagc tatcggagcg acggtcctga
     1081 ccactggacg gccggcacgt tgttcccgcg ttcgtcaaag aagaccgcgc gcgctatcaa
     1141 cgcctgcagc ggtaaccggc cgctcgtggt gctggccaat ctgtccggct tcgacggctc
     1201 gccggagtcc cttgccaaca tgcagctcga gtacggggcc gaaatcggtc gggcgatggt
     1261 gaattttgtt ggcccggtgg tcttttgcgt catctcgcgc taccacggcg gagcgttcgt
     1321 cgtcttctcc ggagttctca acgacaacat cgaggtgatc gcaatcgagg gctcgttcgc
     1381 ctcagtgatc gggggcgcgc cggccgctgc ggtggtgttt tccagcgagg tcaataggcg
     1441 cacggctgcc gacccgcgcg tgcgcgatct cgagactgcg ctgacgacgg cggacgacgt
     1501 ggagcggcgc agtctgcgtg atgaactcgc ggccgcgagg accgcggttc gctcggcgaa
     1561 gctcggcgaa gtcgcggccg agttcgagca cgtgcacaac atcgaacggg cgctgcaggt
     1621 gggttccgtg cacgccatca tcgcggcccg tcggcttcga ccgcacctca tcgacgcggt
     1681 cgagagaggg atggaacgct ccgtgggccg gtagcgctgc tcgctcacgc acgcccggcc
     1741 cacagaactt ctggaggaat ctgcatgcaa gcctttgttc tcaaccgcca cgggcaaatg
     1801 gtgtttccgt ccaacctcat gccagagctc gacttctcgg cgttggagag cctcgagcaa
     1861 ctcgaccgcg tgatccgccg cgacttcgag acaaaggcgc ccacgggcac cgacatcatg
     1921 aaccacctcg agaacgggac ctacgaaagt cgctacgcgc tactgcgcga tatcgccctc
     1981 aacctgttct gggtgaaccg tttcgcgatg accatgtacg acaagcggcc cacgcggtgg
     2041 ggcgacgtgc cccggacgcg atcggacgta ttcctaccgg cgctcacgcc atgggaggac
     2101 ggcgacagga aggtcgcggc cgtccgagcc gcgtacccga cgcttccccc gcggtgggat
     2161 gtcgccgtcg aggaccgcat tttcgatgtc ctcttcgacg tctacggaca tcgcaagcac
     2221 cacgggacca cgctgcccgc catcaagccg accgttgccg agtgcctggc caatcccagt
     2281 accctgacgt tccgcttgcc gaagtacgat cctgactacc ccgtctacga gttctccgcc
     2341 atcgtcgact gctacgagga cgtgcccgag ctgcaggctt tgcatcgctg ggcgatggtt
     2401 ttgcacaacc agtacccatg ggatcgcgcg caggtcgaac tcactgaggt cggtcagctc
     2461 aaggacgacg actacgtggt gctgttctat ccgcgtgaca aggaggtcag gggctttctg
     2521 cgtcgtctgc aacgcgtcgc gaccgcaccg gcgacgccgg cggcggcgga ggcggaggcg
     2581 aagcgacccg tgcggcctta cgctgcgatc gacatccgcc ggcagttcac ggtcatgccg
     2641 agaatagagg cgttggccgt cgtgcacggc gatcagatct gcagcaacga ggatctgatt
     2701 cgcaacgctg cctacaactg gtcgccgatg ggtgccgacg agatccgtga aaagacgggt
     2761 atcgagcagc gtcgttacac ctcgttgacc atggaggaac tggcgctgcg ggccgcacag
     2821 gccgcgctgg ccaaagccca gcgcgggccc gaggagatcg gtgccgtgct ggtgtgcacg
     2881 tgcacgagct cgcgactgat cccttccgtc gcgacctaca tctctgggca actcgggatc
     2941 taccagacgc acgcgtcgtg cgacatcgtc gctgcctgcg ccggcttgcc gtacggcctc
     3001 gccgaggcga cgaggctgct gcaggaggtt gagcggccgg tcttgctcgt gtgcgtagag
     3061 aaattctccg acaagatcgg caatgtgcgg ccatcacgca tgattttcgg ggacggcgcg
     3121 ggagc
//
LOCUS       DATCBJ010000516         5081 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_292864, whole genome shotgun sequence.
ACCESSION   DATCBJ010000516 DATCBJ010000000
VERSION     DATCBJ010000516.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 5081)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5081)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5081
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_292864"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..989)
                     /locus_tag="VII50_10475"
     CDS             complement(<1..989)
                     /locus_tag="VII50_10475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HEY5051316.1"
                     /translation="MKVALDIAVVLALVVIEAVFVATEIALISLRDSQVRGLATKGRR
                     GAAVARLTADPNRFLAGVQIGVTLTSLLSSAYGAVTLSAAAGRELEDLGMTHRSAIIL
                     GFLTVTLSISFVTLVLGELVPKRLALQRAEKAAQLAAPTLDRFASLMRPVIWLLSLST
                     NVVVRLLGGDPDARRESISEEELRGLVATHESLGRDERRLIDEVFTAGDRRLREVLVP
                     RTEVEFLDATTTVAQALKLVASGPHSRYPVVRGSHDDVVGFVHIRDLVTTRAEVDPRT
                     VRVADLVRPVKMLPDGKNVLAALSEMRRDRQHLAIVVDEYGGTAGIVTLEDLV"
     gene            complement(1056..1796)
                     /locus_tag="VII50_10480"
     CDS             complement(1056..1796)
                     /locus_tag="VII50_10480"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEY5051317.1"
                     /translation="MADPDIDREAAAWDELGRRDPHWAILSEPGRQGSWDDDEFFATG
                     RAEIDATLKELGDLLTVRTAALDFGCGLGRLSQALAVHFDSVTGVDVAASMVEGARAR
                     NAFPDRVSYVINVAATLPFDDATFDFGYSILVLQHIPPKVAAGYISELVRVLRPGGVV
                     VFQELSHRAPTVHNAVRRVVPKAVLPLLRKARHGWAAPMTMDGVPRGQVTALVEAAGG
                     EVVEVRADASGEPVWKGYRYTVRRLHAR"
     gene            complement(1789..3003)
                     /locus_tag="VII50_10485"
     CDS             complement(1789..3003)
                     /locus_tag="VII50_10485"
                     /EC_number="1.1.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017986747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006102 - isocitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HEY5051318.1"
                     /translation="MAKIKVVNPVVELDGDEMTRIIWHFIKDQLILPYLDIDLEYYDL
                     GIEHRDATDDQVTIDSANAIKKHGVGVKCATITPDEARVAEFGLKKMWRSPNGTIRNI
                     LGGVIFREPIIISNIPRLVPSWTKPIVIGRHAHADQYAATDFVVPGPGTVTISFTPDG
                     EGEPMQFQVAKFAGGGVAMGMYNFDDSIRDFARASLRYGLNRNYPVYLSTKNTILKAY
                     DGRFKDLFAEVFETEFKAEFEAAGLTYEHRLIDDMVAAALKWEGGYVWACKNYDGDVQ
                     SDTVAQGYGSLGLMTSVLMTPDGRTVEAEAAHGTVTRHFRQHQQGRATSTNPIASIFA
                     WTRGLQHRGKLDNTPNVSAFAIALEEVCIETVESGKMTKDLALLVGPDTEWHTTEDFL
                     AALDENLRIKVG"
     gene            complement(3054..4040)
                     /locus_tag="VII50_10490"
     CDS             complement(3054..4040)
                     /locus_tag="VII50_10490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HEY5051319.1"
                     /translation="MTSGARKDRNGKVSVVGAGFYGSTTALRLAEYDIFESVVLTDIV
                     EGKPEGLALDMNQSRPIEGFETAVIGQTTTADGDGYGAISGSDVVIITAGLPRKPGMS
                     RMDLIEVNAKIVRGVAENVAKNAPNAVVIVVSNPLDEMTALTQLATGFPRNRVLGQAG
                     MLDTARFSNFVAAQLDVPVGAVRTLTLGSHGDTMVPVPSACTVTIDGQQRPLTELLSA
                     DVIEDLVTRTRNGGAEIVALLKTGSAYFAPSAAAARMAKAVAQDSGAVMPVCAWVDGE
                     YGISGVYLGVEAELGAGGVRKVVETPLTEFELAALHAAAEAVRAKQADVADL"
     gene            complement(4114..>5081)
                     /locus_tag="VII50_10495"
     CDS             complement(4114..>5081)
                     /locus_tag="VII50_10495"
                     /inference="COORDINATES: protein motif:HMM:NF016047.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cell wall-binding repeat-containing protein"
                     /protein_id="HEY5051320.1"
                     /translation="GGGGGGGGGGGGGGGGGGGGGVAAPIATAPVRIAGADRFGTADA
                     ALAAEFPTAGSAGAVVLATAANYPDALIGTALAAAKNAPLLFVSGSLSAGTTADIVRV
                     LPAGGTVYLLGGTAAIPDSVATALKALGFAVTRYAGTDRYGTALAVAAGLGSPGTVFL
                     ATGNNFPDALSAGPAAAHLHGVVLLTDGSTMTPAVKTYLSAHPGTVYAVGAPAAAADP
                     AASTLTGTDRYATAVAVDTSIFAGGPTTIGVASGLQFPDALSGGVYQAHFGGAMVLSD
                     PAVLPTSTSAYLTSVKTTVTNTAIFGGTTALSTSVQTAINTALGF"
BASE COUNT          865 a         1709 c         1651 g          856 t
ORIGIN      
        1 tcaacgagat cctcgagcgt cacgatgccc gccgtcccgc cgtattcgtc gacaacgatc
       61 gcgaggtgct gacggtcgcg cctcatctcc gacaacgctg cgagcacgtt cttgccgtcg
      121 ggcaacatct tgaccggccg gacgaggtct gcgacgcgca cggtgcgtgg gtcgacttcg
      181 gcacgcgtcg tgacaaggtc gcggatgtgc acgaagccga cgacgtcgtc gtgcgatccg
      241 cggacgacgg gatagcgcga gtgcggaccg gacgcgacca gcttcagcgc ctgcgcaacc
      301 gtcgtcgtcg cgtcgaggaa ctcgacctct gtgcgcggca cgagaacctc acgcagccgg
      361 cgatcccccg ccgtgaacac ctcatcgatg agccgtcgct cgtcgcgacc cagcgactcg
      421 tgcgtcgcga cgagtccgcg cagctcttct tcgctgatgg attcgcgcct cgcgtcggga
      481 tcgccgccca gcagcctgac gacgacgttg gtcgacagcg acagcagcca gatgaccggc
      541 cgcatcaggc ttgcgaatcg gtcgagagtt ggtgctgcga gctgcgcggc cttctctgcg
      601 cgctgcagtg ccaaccgttt cggcacaagt tcgccgagaa ccagcgtcac gaacgagatg
      661 gacaaggtga cggtgaggaa cccgaggatg atggccgacc gatgcgtcat cccgaggtct
      721 tcgagctcgc ggccggcagc agcggacagc gtcaccgcgc cgtaggccga cgagagcagt
      781 gacgtcagcg tcacgccgat ctgcacgcct gccaggaacc ggtttgggtc cgccgtcaat
      841 ctcgcgaccg ccgccccgcg gcgtcccttc gttgcgagcc cacggacctg actgtcgcgc
      901 aatgagatga gcgcgatctc ggtggccacg aacacagcct caataacgac gagcgcgagc
      961 accaccgcga tgtcgagcgc gaccttcatt gcacgactcc gcacggcgcc tgactgcagg
     1021 gcgcctgact atggccaccg tccataacga tcaggctagc gggcgtgcag ccgccggacc
     1081 gtgtaacggt agcccttcca caccggttcg cccgaggcgt cggcccggac ctcgaccacc
     1141 tcgccacccg ctgcctctac tagcgcggtc acttggccgc ggggaacgcc gtccattgtc
     1201 atcggcgccg cccatccgtg gcgtgctttg cgcaggagcg gcaagacggc cttcgggacg
     1261 acgcgccgca ccgcattgtg aacggtcggc gcccgatgcg agagctcctg aaagacgacc
     1321 acgccgcccg gtcgcaagac ccgcaccaac tcgctgatgt agccagccgc gaccttgggc
     1381 gggatgtgct gcaataccag gatggagtag ccgaagtcga atgtcgcatc gtcgaagggc
     1441 agcgtcgcgg ccacgttgat gacgtaggaa acccgatcag gaaaggcgtt gcgagcccgc
     1501 gcgccttcga ccatggacgc cgcaacgtcg acgccggtca ctgaatcgaa atggacggcg
     1561 agtgcctggc tgagtcgacc aaggccacag ccgaagtcga gtgccgctgt acgtacggtg
     1621 agcagatcgc ccagctcttt caaggtggcg tcgatttctg cgcggccggt cgcgaagaac
     1681 tcgtcgtcgt cccagctgcc ctgccggccg ggctcggaaa ggatcgccca atgcggatcg
     1741 cggcggccca actcatccca ggccgcagct tcgcgatcga tatcggggtc agccaacttt
     1801 gatgcgcagg ttctcgtcga gagcagccag aaagtcttcc gtggtatgcc attcagtgtc
     1861 tgggccaacc agcagagcga ggtccttcgt catcttcccg ctctcgaccg tttcgatgca
     1921 gacctcctcg agtgcaatgg cgaaggcgct cacgttgggg gtgttgtcga gcttgcctcg
     1981 gtgctgaagg ccacgcgtcc acgcgaagat cgaggcgatg ggattcgtcg atgtcgcgcg
     2041 gccctgctgg tgctggcgga agtgccgcgt caccgtgccg tgcgcggctt cggcctcgac
     2101 ggtgcgaccg tccggggtca tcaagaccga cgtcatcagg ccgagcgatc cgtatccctg
     2161 cgcgaccgtg tctgactgca cgtcgccgtc gtagttcttg caggcccaga cgtagccgcc
     2221 ctcccacttc aacgcggccg cgaccatgtc atcgatgagg cgatgttcat aggtgagccc
     2281 agccgcctcg aactcagctt tgaactcagt ctcgaacacc tcggcgaaca ggtccttgaa
     2341 gcgaccgtcg taggccttca ggatcgtgtt cttcgtcgac aggtacaccg ggtagttgcg
     2401 gttgagtccg tagcgcagcg acgcgcgcgc gaagtcgcgg atcgagtcgt cgaagttgta
     2461 catgcccatg gcgacgccgc cgcccgcgaa cttggcaacc tggaactgca tcggctcacc
     2521 ctcgccgtcc ggcgtgaagg agatggtcac cgtgcccgga ccaggaacaa cgaagtccgt
     2581 ggctgcgtac tgatccgcgt gggcgtggcg gccgatgacg atcggcttgg tccagctggg
     2641 aacgagccgc ggaatgttgc tgatgatgat cggctcgcgg aagatgaccc cgccgaggat
     2701 gttacggatc gtgccgttcg gcgaacgcca catcttcttt aggccgaact ccgccacgcg
     2761 cgcctcatcg ggtgtgatgg tcgcgcactt cacaccgacg ccgtgcttct tgatggcgtt
     2821 cgccgaatcg atggtgactt gatcgtcggt cgcgtcgcga tgctcgatcc cgaggtcgta
     2881 gtactccagg tcgatgtcga gatacggcag gatcagttgg tccttgatga agtgccagat
     2941 gatgcgcgtc atctcatcgc cgtccaactc gacgacagga ttgacaacct tgatcttcgc
     3001 catctgatct ccactacctc gaagcgagca ctaacccgag caccgacgcg aatctagaga
     3061 tccgcgacgt ctgcctgctt ggcgcgaacc gcttcggcgg cggcgtgcaa cgccgcgagc
     3121 tcgaattccg taagcggcgt ttcgacgacc ttccgcacgc cgcccgcacc aagctccgcc
     3181 tccacgccga gatagacacc gctgatgccg tactcgccgt caacccacgc acacaccggc
     3241 atgaccgcac cggagtcctg tgcgactgcc ttcgccatgc gtgcggcggc cgccgacggt
     3301 gcgaagtacg cagaacctgt tttcagcaac gcgacgatct ctgcaccgcc gttgcgagta
     3361 cgcgtcacca ggtcttcgat gacgtcggcc gacagcagtt ccgtcagcgg acgctgctgg
     3421 ccatcgatcg tcaccgtgca tgcagacgga acaggcacca tcgtgtcgcc gtgcgaacca
     3481 agcgtcaggg tacgcaccgc accgacggga acgtcgagtt gagctgcgac gaagttactg
     3541 aatcgtgcgg tgtcgagcat ccccgcctga ccgagcaccc gattcctcgg gaagccggta
     3601 gcgagctgcg tcagcgccgt catctcatcg agcgggttcg acaccacgat gacgacagcg
     3661 ttaggtgcgt tcttcgccac gttctcggca acgccgcgca cgatcttcgc gttgacctcg
     3721 atgaggtcca tccggctcat gcccggcttg cgcggcaggc ccgcggtgat gatgacgacg
     3781 tcggagccgc tgatcgcgcc gtatccgtcg ccgtccgccg tggtcgtctg accgatcacc
     3841 gctgtctcga agccctcgat gggccgcgac tgattcatgt cgagggcgag gccctcgggc
     3901 ttgccctcga cgatgtcggt gagaacgacc gactcgaaga tgtcgtattc ggctaatcga
     3961 agtgcggtgg tcgagccgta gaagcccgcc ccgacgacgc tgaccttgcc attgcggtcc
     4021 ttgcgtgcac cactcgtcac gcccgtcatg ctatggggcg acccgtcacc gggagttgcc
     4081 gggggcatat cgcccccggc aacccatccg ccgctagaag ccgagtgctg tgttgatggc
     4141 tgtttgcacg ctggttgaca gtgcggtggt gccgccgaag attgcggtgt tggtgacggt
     4201 ggttttcacg ctggtcagat aggcgcttgt cgaggtgggg agtaccgcgg ggtcggacag
     4261 caccatcgcg ccaccgaagt gggcctggta gaccccgccg ctgagcgcgt cggggaactg
     4321 caggccgctg gccaccccga tggtggtcgg gccgccggcg aagatcgagg tgtcgaccgc
     4381 gacggctgtc gcgtagcggt cagtgccggt cagcgtgctc gccgccgggt cggctgctgc
     4441 ggctggggcg ccgacggcgt agacggtgcc cgggtgggcg cttaggtagg tcttcaccgc
     4501 gggcgtcatc gtcgacccgt ccgtcagtag caccacaccg tgcaggtgtg cggcagcggg
     4561 tccggcgctt aacgcgtcgg ggaagttgtt cccggtcgcc aagaacaccg tgccggggct
     4621 acccaaccca gcggcaacgg ccagcgcggt gccgtagcgg tcggtgcccg cgtaacgggt
     4681 caccgcgaag ccgagcgctt tcagcgcggt ggcgacgctg tctgggatgg cggcggtgcc
     4741 gccaagcagg tacacggtgc caccggccgg caagacccgc acgatgtcgg ccgtcgtacc
     4801 agcgctcagc gacccggaca cgaacagcaa cggggcgttc ttggccgcgg caagcgccgt
     4861 gccgatcagc gcgtccgggt agttagctgc ggtcgccagc accaccgcgc cggcgctacc
     4921 agcagtcggg aactccgcag ccagcgccgc atcggccgtg ccgaaccggt ccgcgccggc
     4981 gatccgcaca ggtgccgtcg cgatcggggc tgcaacgccg ccaccgccgc caccgccgcc
     5041 accgccaccg ccgccaccgc cgccgccgcc gccaccgccg c
//
LOCUS       DATCBJ010000517         2097 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_474417, whole genome shotgun sequence.
ACCESSION   DATCBJ010000517 DATCBJ010000000
VERSION     DATCBJ010000517.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2097)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2097)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2097
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_474417"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..975
                     /locus_tag="VII50_10500"
     CDS             <1..975
                     /locus_tag="VII50_10500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HEY5051321.1"
                     /translation="ETAAEELVLSAGHGGGALTHRTVERIVQRLGFTLHHAADLPHST
                     RSVTDLENRRIYLPPQSIPGGHGLRSLALQAVAHRALEHTNPTTYADFLKQRMEITYF
                     AACCLMPKAAAVATLQRAKSEKDLSVEDFRDVFGVTHESASHRFTNLATSQLGIPVHF
                     LRVSRDGTLLKGYENDGVNFPADVTGAIEGQTICRHWTSRQVFDHGEDAGGFSQYTDT
                     PAGTFWCSAHTGKGTDGEFSVTLGVPFASSKWFRGRETTSRTMSSCPDPACCRRPAAA
                     LSRKWSDASWPSAKLHTHILGALPSGTFPGVDDAEVYAFLESHAPGSS"
     gene            complement(1064..1819)
                     /locus_tag="VII50_10505"
     CDS             complement(1064..1819)
                     /locus_tag="VII50_10505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005883581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY5051322.1"
                     /translation="MNMFDLSGRLAIVTGARRGIGYAMAEALAAAGANIIGVSAALEP
                     EGSAIGTAVLGHGRSFEARTADFADRDAVLALGTELAKRAPDILVNNAGTIERASAID
                     HPLASWDRVLEIDLTSQFILTQALARPMIARGYGKVIFTASLLSIQGGINVPGYTAAK
                     SGLAGLTKALANEWAGLGVNVNAIAPGYVATDNTQALRGDPDRSRSILERIPAGRWGQ
                     PSDLAGATVFLASAASDYVNGITLPVDGGWLGR"
     gene            complement(1816..>2097)
                     /locus_tag="VII50_10510"
     CDS             complement(1816..>2097)
                     /locus_tag="VII50_10510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5051323.1"
                     /translation="IVVVGIHPEPRGVDLQRIFWRELTIVGARVYEKSDFETAVELVS
                     SGVIPTRALITRVVPLAETQGGLDNLEAGTAMKVLVDVRAGQPDEDASR"
BASE COUNT          373 a          698 c          673 g          353 t
ORIGIN      
        1 gaaacggcgg ccgaagaact cgtgctgtcg gcagggcacg gcggcggggc actcacgcac
       61 cgaacagtcg agcgcatcgt gcagcggctg ggattcaccc tgcatcacgc tgccgacctc
      121 ccgcactcaa cccgcagcgt caccgacctg gaaaaccgac ggatctacct tccgccacag
      181 tcaatcccag gcggccatgg cctacggtca ctggcgttgc aggcagttgc tcatcgcgcg
      241 ctcgagcaca cgaatccgac gacctacgcg gactttctca agcagcgcat ggaaatcacg
      301 tacttcgcgg cctgctgcct gatgccgaag gcggcggccg tagcgacgct gcaacgggca
      361 aaaagcgaga aagacctgtc ggtcgaggac tttcgcgacg tgttcggcgt cacccatgaa
      421 agcgctagtc accggttcac caacctcgca acgtcacaac tcgggattcc cgtccacttc
      481 ctacgggtca gccgcgacgg caccttgctg aagggttacg agaacgacgg cgtcaacttc
      541 cctgccgacg tgacgggcgc catcgaaggc cagaccatct gccggcactg gacgtcgagg
      601 caagttttcg accacggcga ggacgccggc gggttcagcc aatacaccga cacccccgcg
      661 ggcacgttct ggtgcagcgc gcacaccggc aaaggaaccg acggcgagtt ctccgtcacc
      721 ctcggcgtgc ccttcgcgtc gtcgaaatgg ttccgcggcc gcgagaccac ctcgcgcacc
      781 atgtccagct gcccggaccc tgcgtgctgt cgccgtccgg ccgccgcact gtcgcgaaaa
      841 tggtccgacg cgtcatggcc cagcgcgaag ctgcacaccc acatcctcgg cgcgcttccg
      901 tcgggcacat tcccgggcgt ggacgacgcg gaggtgtacg cgttcctcga gtcgcacgcg
      961 ccaggctcaa gctaaccggt gacgcccacg aagtggatgc tgccagcaac tgtcatggga
     1021 gaggcgcggg aacgttgcga gccggcgacc gttgctcgtt ctgtcaacgc ccgagccaac
     1081 cgccgtctac cggaagggtt atcccgttga cgtagtcgga cgcggccgag gcgaggaaga
     1141 ccgtcgcacc ggccaggtca gacggttggc cccagcgccc ggctggaatg cgctcgagta
     1201 tggagcgact acggtctggg tcgccgcgga gcgcttgggt gttgtctgta gcgacgtacc
     1261 ccggcgcgat cgcgttgacg tttaccccga ggccggccca ctcgttggct agcgctttgg
     1321 tgagtccggc caggcccgac ttggccgccg tatatcccgg aacgttgatc ccgccctgaa
     1381 tgctcaacaa gctcgcggta aagatcacct tgccgtatcc gcgcgcgatc atggggcggg
     1441 cgagcgcctg ggtcaggatg aattggctcg tcaagtcgat ctcgaggacc cggtcccagc
     1501 ttgcaagcgg atggtcgatc gccgaagcgc gttcgatcgt gcccgcgttg ttgacgagga
     1561 tgtcgggtgc tctcttcgcc agttcggtgc caagagcgag cacggcgtcg cgatccgcaa
     1621 agtccgctgt gcgcgcttcg aacgaccgac cgtgacccaa cacggcggtg ccgatcgcgc
     1681 tgccttctgg ttcgagagcg gcgctgacac cgatgatgtt tgccccggcg gccgccagtg
     1741 cctctgccat ggcgtagccg attcctcgtc gggcgcctgt gacgatggcg agccggccgc
     1801 tcagatcgaa catgttcatc gggatgcgtc ttcgtcaggc tgcccggcgc gaacgtcgac
     1861 gagcactttc atcgccgtac cggcctcgag gttgtccaac ccgccttgcg tttcggcgag
     1921 aggtacgacg cgggtgatga gcgcgcgggt ggggatgacg ccagagctga cgagttcgac
     1981 ggccgtttcg aagtcagact tctcgtacac gcgggcgccc acgatggtca gctcccgcca
     2041 gaagatcctt tggagatcaa cgccgcgagg ctcgggatga atgccgacga cgacgat
//
LOCUS       DATCBJ010000518         4162 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_344879, whole genome shotgun sequence.
ACCESSION   DATCBJ010000518 DATCBJ010000000
VERSION     DATCBJ010000518.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 4162)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4162)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4162
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_344879"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(32..3040)
                     /locus_tag="VII50_10515"
     CDS             complement(32..3040)
                     /locus_tag="VII50_10515"
                     /EC_number="2.7.7.42"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008882 - [glutamate-ammonia-ligase]
                     adenylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional [glutamine synthetase]
                     adenylyltransferase/[glutamine
                     synthetase]-adenylyl-L-tyrosine phosphorylase"
                     /protein_id="HEY5051324.1"
                     /translation="MTNGDQDRHASRQARLARLGFTDATRAGVLAARAELGFGLLTEI
                     AASADPDLALESVVHLVEATQTSFALDDQHNAVALHNALQTDVDFRHRLFAVLGSSAA
                     LGDHLATHPVDWTELADPGVDSSRPSWLGLRSTLTAAVAGKSGPIAEAALRSTYRRLL
                     LRLAARDLSGTATVDDVAAELADLAAATLDAALVVAQAELPSDAAPCRLAVIGMGKCG
                     GRELNYISDVDVVFVGEASAIEGGDVDEVAALRTATQLASGIIRICASIWEVDAALRP
                     EGKVGPLVRTLASHVAYYQRWAKTWEFQALLKARPVAGDLKLGQAYIEAVRPMVWSAA
                     GREHFVEDVQSMRRRVEASLPPGEAEREIKLGPGGLRDVEFAVQLLQLVHGRADDLLR
                     SVTTLNALADLAAGGYVGRQDAALLAEAYRFLRILEHRLQLHRLRRTHMLPLADADVR
                     RLGRSMGFRRDPVGELLAEHARHAREVRRLHEKLFYRPLLAAVARLPGDEARLSLDAA
                     AARLEALGYADPENALRHLEALTSGVSRRAAIQRTLLPVMLGWFADAPDPDGGLLAFR
                     QVSDSLGDTHWYLRLLRDEGAAAQHLALVLASSRYAADLFMRAPESVAMMADTGDGPH
                     QLRPRAAADLRAELLSAVRRYDDAGEAASTARGLRRRELLRVACADLLGLLDTIEVGQ
                     ALSDVTSATLDAVLDAVLRQVQDDRAQRGTPGPLPMKIAIIGMGRLGGGEQGYGSDAD
                     VLFVHEPVDGADEAHAAAVAHLVANELRRLLAVPAPEPPVDVDADLRPEGRQGPLTRS
                     LGSYAAYYARWSKVWESQALLRAAPVAGDADLGARFIEAITPIRWPPEGLAPADVREV
                     RRIKARVEKERLPRGADPTLNTKLGRGGLTDVEWTVQLLQLRFAGEVEGLRTTSTLLA
                     LDAAVDGGLIDDLDAETLASAWRLATRVRNALVLARGKPADSIPTDFRDVVRIARMVG
                     YSPSQSGDFLDVYRRVTRRSRAVVDKVFYA"
     gene            complement(3071..>4162)
                     /locus_tag="VII50_10520"
     CDS             complement(3071..>4162)
                     /locus_tag="VII50_10520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018686332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase"
                     /protein_id="HEY5051325.1"
                     /translation="MFCDILMPDGSPSWADSRYVLKRALARASELGFTFYTHPEIEFF
                     LFERAPKPGEVPIPADSSGYFDHTPHGVGHDFRRQAITMLESMGISVEFSHHEGAPGQ
                     QEIDLRYADALTTADNIMTFRQVMKQVALEQDVYASFMPKPFTEFPGSGMHTHLSLFE
                     GDRNAFFEAGAQYQLSKTARAFIAGILRHASEITAVTNQFVNSYKRLWGATSLSAGAG
                     GEAPSYVCWGHNNRSALVRVPMYKPLKGNSTRVEIRSPDPACNPYLAFALLLGAGLKG
                     IEEGYDLPEGAEDDVWALTEAERRAMGIKPLPASLAEAIAVMETSELVANVLGEHVFD
                     FFLRNKREEWADYQRQVTAYERDRYLPIL"
BASE COUNT          687 a         1429 c         1400 g          646 t
ORIGIN      
        1 gacccacgac ttcgttcccc tacgacatct gtcacgcgta gaacaccttg tcgacgacgg
       61 cccgggatcg ccgggtcaca cgacggtaca catccaagaa gtcaccggat tgggacggag
      121 agtagccaac catacgagcg atccggacga catccctgaa gtccgtcggt atcgaatcgg
      181 ccggcttccc gcgggcgagc accagagcgt tgcgcactcg cgtcgcgagc cgccacgcag
      241 acgccagcgt ttcggcgtcc aggtcatcga tgagaccgcc gtcgacggcg gcgtccagtg
      301 ccagcagcgt cgaggtcgtc cggagtcctt cgacctcccc ggcaaagcgc agctgaagca
      361 gttggacggt ccattcaacg tcggtgagtc cgccccggcc gagcttggtg ttcaacgtcg
      421 ggtcggctcc ccgcggcagc cgctccttct cgacccgcgc cttgatccgc cgcacctccc
      481 gcacgtcagc cggcgccagg ccctccggtg gccagcggat cggggtgatc gcttcgatga
      541 accgcgcgcc caggtcggca tcgccggcga ccggggctgc gcgcagcagc gcctggctct
      601 cccagacctt cgaccagcgg gcgtagtacg cggcgtagga gccaaggctg cgcgtcagtg
      661 ggccctgccg gccttcggga cgcaggtcgg catcaacgtc gacgggtggt tcaggggccg
      721 ggacggcgag gagccggcgc aactcgttcg cgaccagatg cgcgacggca gcggcgtgcg
      781 cctcgtcggc tccgtcgacg ggttcgtgca caaacaatac gtcggcgtcg ctgccgtaac
      841 cctgctcgcc gcccccgagg cggcccatcc cgatgatggc gatcttcatc ggcagcgggc
      901 cgggcgtgcc tcgctgcgcg cgatcgtcct gcacctggcg aagcaccgca tcgaggaccg
      961 cgtcgagcgt ggcggaggtg acgtcggaca gcgcctgccc gacctcgatc gtgtcgagca
     1021 ggccgagcag gtctgcgcac gccactcgca gcagttcgcg gcgccgtagg cctcgtgccg
     1081 tcgacgcggc ctcgccagca tcgtcgtagc gacgcacggc cgacagcagt tcagctcgca
     1141 gatcggctgc cgcccgaggc cgcaactgat gtgggccgtc gccggtgtcg gccatcatgg
     1201 ccaccgattc aggggcccgc atgaacaggt cggccgcgta gcgactgctg gccagcacca
     1261 gcgccagatg ctgcgccgcc gcgccctcgt cccgcagcaa tcgtaggtac cagtgcgtgt
     1321 cgccaagcga gtcagagacc tgacggaagg ccagcaaccc gccgtccggg tcgggagcgt
     1381 cggcgaacca gccgagcatg acgggcaaca acgtgcgctg gatggcggcc cgccggctga
     1441 cgccactcgt taacgcttcg aggtgacgca gcgcgttctc gggatcggcg tatcccagtg
     1501 cttcgagccg cgccgccgcg gcgtcgagcg acaggcgtgc ttcgtcgccg ggaagccgtg
     1561 ccacggccgc gagaagtgga cggtagaaga gtttttcgtg tagccgccgc acttcgcggg
     1621 cgtgccgggc gtgctcggcg agtagttcgc caaccggatc ccgccgaaat cccatgctgc
     1681 gaccaagcct gcgcacatcg gcatccgcga gcggcaacat gtgggtgcgc cgaaggcgat
     1741 gcagctgaag gcgatgttcg aggattcgca ggaaccgata cgcctcggcc agcagtgcgg
     1801 cgtcctgtcg accgacgtat cccccagcgg caaggtcggc cagcgcgttc aaggtggtca
     1861 cgctgcgcag caggtcgtct gcgcggccgt gcacgagctg caacaactgc accgcaaact
     1921 cgacgtcgcg cagaccgccc ggtccgagct tgatctcgcg ttcagcctcc cccggcggca
     1981 ggctcgcctc gacgcgccgc cgcatcgact gaacgtcctc gacgaaatgc tcgcgacccg
     2041 cggcgctcca caccatcggt cgcacggcct cgatgtaggc ctgacccaac ttgaggtcac
     2101 ccgcaaccgg ccgtgcttta agtagggctt ggaactccca ggttttcgcc cagcgctggt
     2161 agtacgcgac gtggcttgcc agcgtgcgga ccagcgggcc caccttgccc tcggggcgca
     2221 gtgccgcatc gacctcccag atcgatgcgc agatccggat gatcccggat gcgagttggg
     2281 tcgcggtgcg cagggcggcc acttcatcga cgtccccgcc ttcgatcgcc gaggcttcgc
     2341 cgacgaacac cacgtcgacg tcgctgatgt agttgagctc gcgacccccg cacttgccca
     2401 tgccgatcac cgcgagccgg cacggtgccg catcggatgg cagctcggct tgcgccacga
     2461 cgagcgccgc gtcgagggtg gcggcagcga ggtcagcgag ctcggccgca acgtcgtcga
     2521 cggtggcggt cccgctgagg tctcgcgcgg cgagacgcag cagcaggcgg cggtacgtcg
     2581 accgcagggc ggcctcggcg atcggtccgc tcttcccggc gaccgccgcg gtcaacgtcg
     2641 accgaaggcc gagccagctc ggccggctgc tgtcgacgcc ggggtccgcg agctcggtcc
     2701 agtcgaccgg atgcgtcgcc aggtggtcgc cgagcgccgc gctcgacccg agcacggcga
     2761 acaaccggtg ccggaagtcg acgtcggtct gcagggcgtt gtgcagggcg actgcgttgt
     2821 gctggtcgtc aagcgcaaag gacgtctggg tggcttcgac cagatgcacg acggactcca
     2881 acgcgagatc gggatccgcg ctagcggcaa tctcggtcaa gagaccgaac ccgagctcgg
     2941 ctcgggccgc aaggaccccc gcacgcgtcg catcggtgaa gcccagtcga gcaagcctgg
     3001 cctggcgact ggcgtgccga tcctggtcgc cgttggtcac gccttgacgc tatctgtcgc
     3061 gcccgatgag ctagaggatc ggcaggtacc ggtcacgctc gtaagcggtg acctggcgct
     3121 ggtagtccgc ccactcctcg cgcttgttac gcaagaagaa gtcgaacacg tgctcgccga
     3181 gcacgttcgc gaccaactcg gaggtctcca tcacggcgat ggcctcggcg agcgacgccg
     3241 gcagcggctt gatgcccatg gcgcggcgct ccgcctccgt cagcgcccac acgtcgtcct
     3301 cggcgccttc gggaaggtcg tatccctctt cgatgccttt caggccagca ccaaggagta
     3361 gcgcaaaggc gagataggga ttgcacgccg ggtcgggcga ccggatctcg acccgcgtcg
     3421 agttgccttt cagcggcttg tacatcggca cccgcaccag cgccgaccgg ttgttgtgac
     3481 cccagcacac gtacgacgga gcctcgccgc cggcgccagc cgaaagtgac gtcgcgcccc
     3541 acaaccgctt gtagctgttg acgaactggt tggtgaccgc ggtgatttct gacgcgtgcc
     3601 gcaagatgcc cgcgatgaac gcgcgcgccg tcttcgaaag ctgatattgc gcaccggcct
     3661 cgaagaatgc gttgcggtcg ccctcgaaca gcgacaagtg ggtgtgcatg cccgagccgg
     3721 gaaactcggt gaacggcttc ggcatgaacg aggcgtagac gtcctgctcg agcgcaacct
     3781 gcttcatgac ctggcgaaac gtcatgatgt tgtcggccgt ggtcagcgcg tcggcgtaac
     3841 gcaggtcgat ttcctgctgt cccggagcac cctcgtggtg gctgaactcg accgagatgc
     3901 ccatcgattc gagcatcgtg atcgcctgcc ggcgaaagtc gtgcccgaca ccgtgcggcg
     3961 tgtggtcgaa gtacccgctt gagtcggccg ggatcggaac ctcaccaggc ttcggcgccc
     4021 gctcgaacaa gaagaactcg atctcggggt gggtgtagaa cgtgaatccg agctcggagg
     4081 cgcgggcgag cgcgcgcttt agcacgtagc gggagtccgc ccaactcggc gatccgtcgg
     4141 gcatcaagat gtcgcagaac at
//
LOCUS       DATCBJ010000519         3919 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_86495, whole genome shotgun sequence.
ACCESSION   DATCBJ010000519 DATCBJ010000000
VERSION     DATCBJ010000519.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 3919)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3919)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3919
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_86495"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1160
                     /locus_tag="VII50_10525"
     CDS             <1..1160
                     /locus_tag="VII50_10525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5051326.1"
                     /translation="PPVHAWAALRVFEVSGGTDYDFLERAFHKLLLNFTWWVNQEDHD
                     GNNLFQGGFLGLDNIGPFDRSRLSPDAGELEQSDGTAWMAMFCLDLLDMALVLAMHDP
                     TYEDVATKFFEHFAHIATAAQQQGLWDEADGFFYDQLRLPDGQRLPIRVRSLVGLIAV
                     CAVSHVSNSSLAALPGFASRLKWFTTNRPLLTRCVAETVDDDTLFSMVDPGQLRRLLS
                     RMFDEAEFFSPHGIRSISAYHRDHPFMFTVDGQDFRVDYEPGESSTGLFGGNSNWRGP
                     VWLPLNALLVEALERFDKHLGADFLVEVPTGSGRRVRLGDAADELSRRLVGLLLPADD
                     GKLPASGNRSWPDGLLWFHEYFHGDTGEGLGASHQTGWTSLLAHLVLTRRS"
     gene            1244..1804
                     /locus_tag="VII50_10530"
     CDS             1244..1804
                     /locus_tag="VII50_10530"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SigE family RNA polymerase sigma factor"
                     /protein_id="HEY5051327.1"
                     /translation="MLRSPASELDDEFRSLVLGSQARILRFAELLTGDRHVAEDLTQH
                     SFAKLYASWRRLHTGNAEQYVRRCVINANTDRLRRGTWRESSRADIPERPDHFDLVAS
                     LAEHDFVMRALAELTQIERAVIALRYYNELSEAEIAGELNMPAGTVKSTAARALRKLR
                     DVVNQARATRTDNDHAASGNDQGAAR"
     gene            1801..2556
                     /locus_tag="VII50_10535"
     CDS             1801..2556
                     /locus_tag="VII50_10535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5051328.1"
                     /translation="MSAFPGGSSALSDECPINLHYVDPRPNFVDVDVVVRDGRRIRRR
                     RTTTVSLASATMVAAVVAVSLGAAHVFAHRDTVQVAGIYKSSFLKSYPPVDSAVVVLG
                     TYADASQSNQSAIIWMTQSGGLCYGSADMSARAADSSFFGSCSSPAPAELTTPGPTEL
                     VPGWPVFQPTPSKVGEFVAIGLLRGTAATVEVTANGQTAEAPVHPLATTNGEAAGAYA
                     VWLRAEPDGSIHSADITNIWALDGSGHVVAQIP"
     gene            2920..>3919
                     /locus_tag="VII50_10540"
     CDS             2920..>3919
                     /locus_tag="VII50_10540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DnaB-like helicase C-terminal domain-containing
                     protein"
                     /protein_id="HEY5051329.1"
                     /translation="MSVIELPGSSSREVDRTPPQDINAEQSVLGGMMLSKDAIADVVE
                     ALHGGDFYRPAHQTVFEVVLDLYGRGEPADAVTVAAELSKRGEIARVGGAPYLHTLIS
                     SVPTAANAGYYAKIVAERAILRRLVEAGTRIVQLGYSGVGNEVDDIVDRAQAAIYDVT
                     ERRQTEDYSILESLLQPTMDEIEASGHRGGGLTGVPTGFADLDSLTNGLHPGQLVVIA
                     ARPAVGKSTMALDIARAASIRHNQTSVLFSLEMSKHEITMRLLSAEARIPLQTMRTGQ
                     MRDDDWVKLTRRLGEMASAPLYIDDSPNMTMMEIRSKARRLKARHPNLGLIIVDYLQ"
BASE COUNT          696 a         1263 c         1261 g          699 t
ORIGIN      
        1 acccgccggt acacgcatgg gccgcgctgc gggtgttcga ggtgtctggc ggaacggact
       61 acgacttcct cgagcgggcg ttccacaagc tgttgctcaa cttcacctgg tgggtgaacc
      121 aggaggatca cgacggaaac aacctcttcc agggtgggtt cctcggtctc gacaacatcg
      181 gtccgttcga ccgttcgaga ctttcgccgg acgccgggga gctggagcaa agcgacggca
      241 ccgcgtggat ggcgatgttc tgcctcgact tgctcgacat ggccctcgtg ctcgcgatgc
      301 acgaccccac ctacgaagac gtcgcgacga agttcttcga acacttcgca cacattgcca
      361 cagcagcgca acagcagggc ctgtgggacg aagccgacgg gttcttctac gaccagttgc
      421 ggctgccgga cgggcagcgg ctacctatta gggttcgatc gctcgtcgga ctgatcgcgg
      481 tgtgcgcggt cagccacgtc tccaactcgt cgctggccgc gttgccgggg ttcgcgagtc
      541 gcttgaagtg gttcacgacg aaccggccgc tgttgacacg ctgcgtggcc gagacagtgg
      601 acgacgacac actgttctcg atggttgatc cgggtcagtt gcgcaggctg ttgagccgga
      661 tgttcgacga ggccgagttc ttttcaccgc acggcatcag gtcgatctcg gcgtatcacc
      721 gtgatcaccc gttcatgttc acggtggacg gtcaggactt tcgggtcgac tacgagccgg
      781 gcgaatcgtc gacaggcttg ttcggtggca actccaactg gcgcggtccc gtgtggctgc
      841 cgctgaacgc gctgctggtg gaggcgctcg aacgcttcga caagcatctc ggggcggact
      901 tcctggtcga agtcccgacc ggatcgggcc gccgggtcag gctcggcgat gccgcggacg
      961 aactctcccg ccggttggtg ggactgctgc tgccagcgga cgacgggaag ctgccggcgt
     1021 ccggtaatcg ctcgtggccc gatgggctgc tgtggtttca cgagtacttc cacggcgaca
     1081 ctggcgaagg gctcggtgcc tcacaccaga ccgggtggac ctcgctgctc gcgcacctgg
     1141 tgctgacccg ccgttcgtga gccggtaggc cggcccgctg cgagaaccgg acccgccccg
     1201 actgcgttct agttgtcagg gccatgcgtg ggaggaggga tcggtgctga ggtcgcccgc
     1261 tagcgaactt gacgacgagt ttcgaagctt ggtgctgggc agccaagcgc ggatcctgcg
     1321 gttcgcggaa ctccttaccg gcgatcggca cgtcgccgag gacctcacgc agcacagctt
     1381 cgccaagctc tacgcgtcgt ggcgccgact ccacaccgga aatgctgaac agtacgtccg
     1441 ccgctgcgtc atcaacgcca acaccgaccg gttgcgtcgt ggcacgtggc gcgagagctc
     1501 gcgggccgac atccccgagc ggcccgacca cttcgacctt gtcgcgtcac ttgccgagca
     1561 tgatttcgtg atgcgggcgc tcgccgagct aacgcagatc gagcgagcgg tgatcgcgct
     1621 tcgctactac aacgaactca gcgaggccga gatcgccggc gagctcaata tgcccgccgg
     1681 cacggtcaag agcaccgcag ctcgagcatt acgcaagctg cgcgacgtgg tgaaccaggc
     1741 gcgcgcaact aggaccgaca atgaccatgc tgcctccggc aatgaccaag gagccgctcg
     1801 atgagtgctt tcccgggtgg ctcatccgcc ctgagcgacg agtgcccaat caacctgcat
     1861 tacgttgatc caaggcccaa cttcgtcgat gtcgacgtgg ttgtgcggga cggtcgccgg
     1921 atccgccgcc gccggacgac gacggtgtcg ctcgcatcgg ccacaatggt cgcagccgtt
     1981 gtcgcagtgt cactgggtgc agcgcacgtc ttcgctcatc gcgacaccgt tcaagtcgcg
     2041 gggatctaca agtcgagctt cttgaagtca tacccgcctg tcgattccgc tgttgtcgtt
     2101 ctcggcacct atgccgacgc gtcccagtca aaccagtcgg cgatcatatg gatgacccaa
     2161 agcggtgggc tctgctatgg cagcgccgac atgtctgcac gcgcggcaga cagctctttc
     2221 ttcggttcct gcagctcccc ggcgccagct gagctgacga cgccggggcc aacggagttg
     2281 gttcccggat ggcccgtctt ccagccgacg ccgagcaagg tgggtgagtt cgtcgcgatc
     2341 ggtctcctgc gcggcactgc cgccacggtg gaggtaaccg cgaacggcca aactgcggaa
     2401 gcgccggtgc atccgctcgc gacgactaac ggtgaagcgg ctggtgccta cgccgtctgg
     2461 ctccgcgccg agcccgacgg ctcgattcac tcggcggata tcacgaacat ctgggcgctc
     2521 gacggcagcg ggcacgttgt tgcacagatc ccgtaggaac gaccgcgtcg actccgggac
     2581 gctcagtctg gtcgtaggtg ggtgtccacg agctgagacg gttccgtgaa atcgctcaga
     2641 tttctcctgt ccacagtcgg tggacggcgt ttggcgaggc ggcagcggcc agtcgcgagt
     2701 aggcacttcg gtcctgcaca ccgtcgtcca caccgtgtcc ccaaggcagg agcagttgtc
     2761 cccagctagc gcacagatgc gtccgcaacc aggccttccg gcgcaagact ggactcccta
     2821 aggtcctgaa gcgcaggtgc acgccgagcg ccagcgacga attgtcagtg gccacccgta
     2881 cgcatgtcat tcatgagtta ccaggcgcga ggtgcagcag tgagtgtgat cgaactccca
     2941 ggttcgagtt cacgggaagt agaccgcaca ccgccacaag acatcaacgc cgagcagtcc
     3001 gtgctcggcg gcatgatgct gagcaaggac gcgatcgctg atgtcgtcga agcgctgcac
     3061 ggcggtgact tctaccgtcc cgcacaccag accgtcttcg aggtcgtgct cgacctttac
     3121 ggccgtggcg agccagccga cgcagtaact gtggcggccg agctgagcaa gcgcggcgag
     3181 atcgcgagag tcggcggcgc gccgtacctg cacaccctca tctcgtcggt gccgaccgct
     3241 gccaacgccg gttactacgc gaagattgtc gccgagcgcg cgatcctgcg gcggctcgtc
     3301 gaggccggca cccgcatcgt gcagttgggg tattccggcg tgggcaatga ggtcgacgac
     3361 atcgtcgacc gcgcgcaggc agcgatctat gacgtcaccg agcgccgcca aaccgaggac
     3421 tacagcatcc tcgagtcact cttgcagccg acgatggacg agatcgaggc gagcggtcat
     3481 cgtggcggcg gcctcaccgg cgtcccgacc ggctttgccg atctcgactc attgacgaac
     3541 ggcttgcacc ccggccagct cgtcgttatc gcggcgaggc cggctgtcgg aaagtctacc
     3601 atggcactag acattgcacg ggcggcgtcg attcggcaca accagacgtc tgtgctgttc
     3661 tcgctggaga tgagcaagca cgagatcacc atgcgcttgt tgtctgcgga agcgcgcatt
     3721 ccgttgcaga cgatgcgcac cggtcagatg cgcgacgacg actgggtgaa actcacccgc
     3781 cgactcggcg agatggccag cgcaccgtta tacatcgatg actcgccgaa catgaccatg
     3841 atggagatcc gctcgaaggc gcggcgcctc aaggcgcgcc acccgaacct cgggctgatc
     3901 atcgtcgact acctccagc
//
LOCUS       DATCBJ010000520         2513 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_444658, whole genome shotgun sequence.
ACCESSION   DATCBJ010000520 DATCBJ010000000
VERSION     DATCBJ010000520.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2513)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2513)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2513
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_444658"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..777
                     /locus_tag="VII50_10545"
     CDS             <1..777
                     /locus_tag="VII50_10545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY5051330.1"
                     /translation="ASGAGSASAAVPVIRLWQLTKTYGEGEAAVHALRGVSLSVYQGD
                     YVAIMGASGSGKSTLMSIIGCLDVQTGGRFLLDGVDVELLDENELSEIRNRKIGFVFQ
                     SFNLLSGSTALQNVELPLTYAGVRSGERRRRATAALDAVGLARRYGHRPAMMSGGERQ
                     RVAVARAIAVDPSLILADEPTGNLDSVATDDVLAIFARLNTEGRTIVMITHENEVAQH
                     AKRVVRVKDGLIIDDMRQAPIEGPPPLHTLTTYAEAGIRG"
     gene            804..2009
                     /locus_tag="VII50_10550"
     CDS             804..2009
                     /locus_tag="VII50_10550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009071990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY5051331.1"
                     /translation="MAGILHNPMRSLLTMLGMLIGVGSVIMLVAVGTGSSQKVAKQIE
                     GLGTNLLQISKQGGFGGFGGRATTGTQSKPANLTAVDVAALEDNTKNPDVVAVAPVTT
                     ASSVTAVYQGASYSPSSFVGTTPSYEQIKDYQIQEGTFFTAADETQHNRVAVIGETVA
                     TNLFGTADPIGNNVQFNGASYQIIGLLKPKGTNGIQDQDDVVMAPLTAVQDTLTGYST
                     SYSSIDVEAASPAKQANAQAEVTATLETTHGIAAGGTDDFRILNQASLLQTSSSSNKV
                     FTTLLGIVAGISLLVGGIGVMNIMLVTVTERTREIGIRKAIGAKRSDILGQFLVEAVL
                     LSAIGGLVGVAAGIGGSHFRIIGIQPVVADYSVVLAFAVSVVTGLFFGLYPASRAARL
                     LPIEALRYE"
     gene            2036..>2513
                     /locus_tag="VII50_10555"
     CDS             2036..>2513
                     /locus_tag="VII50_10555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5051332.1"
                     /translation="MTQHSTDPANVVGYDSPNGVASSFAPDFDEPPPPTLPRVRRRKL
                     PVATLVLALLAVGAAGFFVGVKVEKSKVPASTSNASRLAAAAAAFGTGATAARASGSP
                     SAGATGTTGAAGTGAAGTGAAGTGAAGGAANIIGTVTVIDGTTLYVTDSSGNVVKVT"
BASE COUNT          456 a          785 c          835 g          437 t
ORIGIN      
        1 gcttctggcg ccggctccgc ctcggctgct gttccggtga tccggttgtg gcagctcacg
       61 aagacctacg gcgaaggcga ggccgcggtg cacgcgctgc gtggcgtgag tctgtcggtc
      121 taccaaggcg actatgtggc gatcatgggt gcgtccggct cgggcaagag cacgctgatg
      181 agcatcatcg gctgcctcga cgtccagacg ggtgggcgat tcctcctcga cggtgtcgat
      241 gtggaactgc tggacgagaa cgagttgtcg gagatccgca accgaaaaat cggattcgtc
      301 tttcagagct tcaacctgtt gtcgggttcg accgcgttgc aaaacgtcga gttgcctttg
      361 acgtatgcgg gtgtgcggtc tggtgaacgg cgccgccggg caaccgcggc actcgacgcg
      421 gttggactcg ccagacggta tgggcaccgg ccggcaatga tgtcgggcgg tgagcgccag
      481 cgggtcgcgg tcgcgcgcgc catcgcggtc gacccgtcgc tgatcctcgc tgacgagccc
      541 accggcaacc tcgactcggt cgcgaccgac gacgtgctcg ccatctttgc tcggctgaac
      601 accgagggtc gcacgatcgt gatgatcacc cacgagaatg aggtggccca gcacgccaag
      661 cgggtcgtgc gggtgaagga cggtctgatc atcgacgaca tgcggcaagc gcccatcgag
      721 gggccgccgc cgttgcacac cctgacgacg tacgcggaag ccggtatccg cggatgatta
      781 gggccgacag ctttcggatc gccatggcgg gtatcctcca caacccgatg cgatcgttgc
      841 tcacgatgct tggcatgctg atcggtgtcg gctcggtcat catgctggtc gccgtcggca
      901 ccgggtcgtc gcagaaagtg gcgaagcaga tcgaaggact tggcacgaac ctgctccaga
      961 tcagcaagca gggcggcttt ggcggcttcg gcggccgggc aaccaccggt acgcaatcaa
     1021 aaccggcgaa cctgacggcc gtggatgtcg cggccttgga agacaacaca aagaaccctg
     1081 acgtcgtcgc cgttgcgccc gtcacgacgg caagctcggt gaccgcggtc tatcagggtg
     1141 cgtcgtactc accgtcctcc tttgtcggca cgacaccgag ttacgagcag atcaaggact
     1201 atcaaattca ggaaggcacg ttcttcaccg ccgcggacga gacccagcac aaccgcgtcg
     1261 cggttatcgg cgagactgtg gcaacgaacc tgttcggcac cgccgacccg atcggcaaca
     1321 acgtgcaatt caacggcgcg agctaccaga tcatcggatt gctgaagccg aagggtacga
     1381 acggaattca ggaccaggac gacgtcgtga tggcgccgct cacggcggta caagacaccc
     1441 tgaccggcta cagcacgtcg tactccagca tcgatgttga ggcggcgtct ccggcgaagc
     1501 aggctaacgc gcaagccgag gtgaccgcga ctctggagac cacgcacggc atcgccgcag
     1561 ggggaactga tgacttccgc attctcaacc aggcaagcct gctgcagacc tcgtcgtcgt
     1621 cgaacaaggt gttcacgacg ctactcggca tcgttgccgg gatcagtttg ctggtcggcg
     1681 gcatcggcgt catgaacatc atgctcgtga cagtgaccga gcggacgcgt gagatcggca
     1741 ttcgcaaggc gatcggcgcg aagcgttccg acatcttggg gcagtttctg gttgaggcag
     1801 tgctgttgtc ggccatcggt ggactcgtcg gcgttgccgc aggtatcggg ggcagccatt
     1861 tcaggatcat cggtatccag cccgtggtgg ctgactattc cgtcgtgctc gccttcgccg
     1921 tgtccgtcgt gaccggcctc tttttcgggc tctacccagc cagtcgggca gcgcgactgc
     1981 tgcccatcga agcactgcga tacgagtgag ccatgaaccc cgaccggaga atctgatgac
     2041 ccagcacagc accgacccgg ccaatgtcgt cggatacgac tcgcccaacg gcgtcgcctc
     2101 gagcttcgcg ccggacttcg acgagccacc gccgccaaca ctgccgaggg tacggcgccg
     2161 caagctgccc gtcgcgactt tggtgctcgc gttgctcgca gtgggtgcgg cgggcttctt
     2221 cgtcggcgtg aaggtcgaga agagcaaggt gcccgcttcg acatcgaacg cctcgcgact
     2281 cgcggccgca gcggctgcgt tcgggaccgg tgcgacggcc gcgcgcgcga gcgggtcgcc
     2341 gtccgctggt gcgaccggga ccaccggtgc ggcaggtacc ggagctgcag gtaccggagc
     2401 tgccggtacc ggggcggccg gcggcgcggc gaacatcatc ggcacggtga cggtgatcga
     2461 cggaacgacg ctctatgtca ctgactcgtc gggcaacgtc gtgaaggtca cca
//
LOCUS       DATCBJ010000521         2353 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_460777, whole genome shotgun sequence.
ACCESSION   DATCBJ010000521 DATCBJ010000000
VERSION     DATCBJ010000521.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2353)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2353)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2353
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_460777"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..491)
                     /locus_tag="VII50_10560"
     CDS             complement(<1..491)
                     /locus_tag="VII50_10560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009078138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HEY5051333.1"
                     /translation="MTAVELAEITADAGEPARSGEQDQPRPWSLSPSRAGDFMTCPLL
                     YRLRVIDRLPEPSSPVTARGTLVHGVLERLFDSPAPERTLDRAASLLEPEWQRLLEER
                     PELIDLFASVEAPAEASTDEACSSDAAAGTDDAVSAWLASARVLLETYFALEDPTRIE
                     PAQR"
     gene            597..1775
                     /locus_tag="VII50_10565"
     CDS             597..1775
                     /locus_tag="VII50_10565"
                     /inference="COORDINATES: protein motif:HMM:NF014244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HEY5051334.1"
                     /translation="MVAPEQQPAKKQNGRPTAPSRGVTYGGLLLGRPFGVPIYISASW
                     LLFVAFITVQFAPVVASAVPGIGAARFVVAAAFGVFLGVSVLAHEVAHCVVARGLGIP
                     IERISLSFLAGHSALAREPETPARSAAVAVAGPATNLVIGVCSFLAYSRLPEGSIALV
                     LAAGLAWSNLLVGVYNLLPGLPLDGGQLLRAALWRITRSPRTGTIGAAWAGRVIAACT
                     AVVGLYLVSTRRTDAQFSALWALLIAAMLWVSSTQVLRQQALRDRLPGISARALSRSS
                     ISVLADLPLAEAVRRAQEAHAQGIVIVDRDGRPTGVVREAAVTATPAPRRPWISTGSV
                     ARNVSTAELINASWSGEELLARLQLAAASEYVVVDPAGAIYGVLAAVDVARALSGSPA
                     "
     gene            1772..>2353
                     /locus_tag="VII50_10570"
     CDS             1772..>2353
                     /locus_tag="VII50_10570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007380868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine-N1)-methyltransferase"
                     /protein_id="HEY5051335.1"
                     /translation="MTRDAAQPPAALIKRGPFEVGDQVQITDPKGRKHTVLLVAGKQF
                     HTHQGALEHDELIGQPEGVVVTSTGGTAYLAFRPLLSDFVLSMPRGATVVYPKDAAMI
                     IGYADVFPGARVIEAGAGSGALSCSLLRAVGETGEVHSYERREDFAAVAAKNVLRFFG
                     SEHQAWRLTVGDLVESLREHPLEHPVDRVVLDML"
BASE COUNT          323 a          794 c          807 g          429 t
ORIGIN      
        1 cgctgtgcgg gttcgatccg ggtcggatct tcgagcgcga aatatgtctc gagcaagacg
       61 cgtgcactag cgagccacgc gctcacggcg tcatcggtac ccgccgcggc atcgctcgag
      121 caggcttcgt cggtggatgc ctcggccgga gcttcgacac tcgcgaacag gtcgatcaac
      181 tctgggcgtt cttcgagcag ccgttgccac tccggctcga gcaaactggc tgcgcggtca
      241 agtgtgcgct cgggtgcggg gctgtcgaac agccgctcga ggacaccgtg caccaacgtg
      301 cctctggcgg tcaccgggct cgatggctcg ggcaagcggt cgatgacccg aagccggtag
      361 agcaacgggc aggtcatgaa atcgccggct cgcgagggcg agagcgacca cggacgcggc
      421 tgatcttgct cgcccgatcg agcaggttca ccggcgtcgg cggtgatttc ggcgagctcc
      481 actgcggtca taccggcagc gtaggcgagc ctggtgacag ttcagctccg ccccgctcac
      541 ctgtgtttca acccgagcgc gcgtgtgggt aactgaccgg cccgtagcat cggtaggtgg
      601 tcgcccccga gcaacagcct gcgaaaaagc agaacggccg gccgaccgcg ccgtcgcgtg
      661 gcgtgaccta cggcggtctc ctgctcggcc gtcccttcgg cgtcccgatc tacatctccg
      721 cgagttggct gttgttcgtg gctttcatca cggtgcagtt cgccccggta gtggccagcg
      781 cggttccggg gattggcgcc gcccggttcg tcgtggcggc ggccttcggg gtgttcctgg
      841 gtgtgtcagt gctcgctcac gaggtggcgc actgcgtggt tgcccgcggg ctcggcattc
      901 cgatcgagcg tatttcgctg agcttcctgg ccggccactc ggccctcgcg cgtgagccgg
      961 aaaccccggc caggtcggca gccgtcgccg tcgctgggcc cgcgaccaac ctggtcatcg
     1021 gggtgtgctc gttcctcgca tatagccggc tccccgaagg gagcatcgcc ctggtgcttg
     1081 cggctgggct ggcgtggtcc aacctcctcg tcggggtcta caacctgttg ccaggtctcc
     1141 cgctggacgg gggccagtta ctgcgggccg ccttgtggcg catcacccgc agcccgcgca
     1201 ccggaaccat cggtgctgcc tgggcgggcc gggtgatcgc ggcctgcacg gctgtcgtcg
     1261 ggctctacct tgtcagtacc cgacggaccg atgctcagtt cagtgccctg tgggcgttgc
     1321 tcatcgccgc gatgctgtgg gtgagctcga cgcaggtgtt gcggcagcaa gcgctgcgcg
     1381 atcggctgcc cggtatctcc gctcgtgcgc tgagccgcag ctcgatctcc gtcctggctg
     1441 acctgccgct cgccgaggcg gtccgtcgcg cgcaggaggc gcatgcccag gggatcgtca
     1501 tcgtcgaccg cgacgggcgc cctaccggag tcgtgcggga ggccgctgtc actgcgacgc
     1561 cggcaccgcg ccgtccatgg attagcaccg gatcggtggc ccgcaacgtt tcgacggcgg
     1621 aactgatcaa cgcgtcgtgg tcgggggagg aacttctcgc ccggctccag ctcgcggccg
     1681 cgtccgagta cgtggtcgtc gacccggccg gtgccatcta tggcgtgctc gccgctgtcg
     1741 acgtcgcgag agcactgtcc ggcagccctg cgtgacgcgc gacgcggcgc agccgcccgc
     1801 agccctgatc aagcgcggtc ctttcgaggt cggcgaccag gtccagatca ccgatccaaa
     1861 gggccgcaag cacaccgtgc tgcttgtcgc cggcaagcag ttccacaccc accaaggtgc
     1921 gctcgaacat gacgagctga tcggccagcc cgaaggcgtc gtcgtcacgt cgactggggg
     1981 cacggcgtac ctggccttcc ggccgttgct ttccgacttc gtgctgtcga tgccacgcgg
     2041 ggcgaccgtc gtctacccca aggacgccgc gatgatcatc ggctacgccg atgtcttccc
     2101 tggggctcga gtcatcgagg ccggggccgg ttctggagcg ctcagttgct ctttgctgcg
     2161 agcggtcggc gagacggggg aggtccattc ctacgagcga cgcgaggatt tcgccgcagt
     2221 ggccgcaaag aacgttctgc ggttcttcgg ttccgaacat caggcgtggc gactgaccgt
     2281 cggcgatctc gtcgagtcgt tgcgcgagca ccctctcgag caccccgtcg accgggtcgt
     2341 tctcgacatg ctc
//
LOCUS       DATCBJ010000522         4388 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_11390, whole genome shotgun sequence.
ACCESSION   DATCBJ010000522 DATCBJ010000000
VERSION     DATCBJ010000522.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 4388)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4388)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4388
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_11390"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1177)
                     /locus_tag="VII50_10575"
     CDS             complement(<1..1177)
                     /locus_tag="VII50_10575"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: protein motif:HMM:NF005301.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent 6-phosphofructokinase"
                     /protein_id="HEY5051336.1"
                     /translation="MVSTADQLAITADDLMVTRLGETTVTSPMTRLLRDRASSVHYVH
                     ESDRVLLDDTVEMAVARNLPAAQLPSFEPGGPRARLYFEPGTTRAGIVTCGGLCPGLN
                     NVIRGLVLELSQNYGVIDIVGFRNGYRGLVSADPPMALTPQVVHDIHNRGGTILGTSR
                     GDQDAHEMVATLIRLDISVLFVVGGDGTLRGAQKLVDAAAALGYELSVVGVPKTIDND
                     IPYLDHSFGFQTAFARAAESIRAAHIEASSTTHGVGLVKLMGRHSGFIACYAALANHD
                     VDFVLVPEVPFELEGDDGFLNHLRRRVHHQGHAVVVVAEGAGQHLLPLDGSRDASGNT
                     RLADIGQLMRDRIVASFADAGSELSLRYVDPGYAIRSVPANAYDAVFCIRLAQAAVHA
                     "
     gene            1320..1991
                     /locus_tag="VII50_10580"
     CDS             1320..1991
                     /locus_tag="VII50_10580"
                     /inference="COORDINATES: protein motif:HMM:NF016101.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HEY5051337.1"
                     /translation="MDSIGWIARTSALTAVLALTGSVGVGIGAGSASAASVPRPAHVV
                     VVLMENHAYGQIIGSTSAPYLNSLAYAGASFTNAHGVTHPSQPNYLALFSGSTQGVTS
                     DSCPHTFPAGNLASELVAAKLGFKAYSESLPAAGSMVCTSGSYARKHAPWTNFTDVPA
                     SRQSRQLSHELHQPADRVVCRPESPTRHARRNNRAGGHLAESAPRFLCHMGEDTQQLA
                     RCDLG"
     gene            complement(2184..>4388)
                     /locus_tag="VII50_10585"
     CDS             complement(2184..>4388)
                     /locus_tag="VII50_10585"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HEY5051338.1"
                     /translation="WLAAVYQSRVPWRVATDLARRTSGNPFFLEELVSSAPGVELEAL
                     CALPLPISVSEAVLRHLDGLPDTQRRIVDSAAVLGRRIPFDLLAIVSGAGEDDLIATM
                     RDLVERGLVTEVEPDVFSFRHALTREAVASRLLGRQRRRLHEKAHAALLEMGSDDWGA
                     LAYHAAGAHLWDDVVDAARRGAAVHLRTGSTYEALRLAELGLQEVDADAVLLELATRA
                     AWACSLLANAANYATQWAALAEAGDNDGELSRALRALARLKWEVGDTDDHAALVTRLL
                     SVADRLPDGPDLAAVFSYVAEVRMLAGQSAEAVEWADRALALMADDDETRAAAMVNKG
                     SAVFDIPGRFDEGAALLVAAIDAAVAAGDVFSALRGLNNLLQGVLVRWPADRVWSHVS
                     RMTELVSSFGRHDWLSGLAGITAAYHSNVLGDPLAARAVLDDALIDLTADAGGKRGWL
                     FAERAWLAGELGDDDAMAAASAMFALAPLTVGGTSTAQDHWVETLSMEAAARVGDVAT
                     VARRLADLGRRAIAAPTGVCNLSSWTWGVELLAAVRAGLAPAEVGRVLELLFISPNRT
                     IRDVDVGWVLHVQAALAAASGEHGAAIELYRSALAEPRRRPGAIVDADVRIGLARSLL
                     VLGNIEEAQTAAAEAEVFLARWTGWRRDELVSLQHRLASGGARVASGQLTARELEVAA
                     LVAAGLSNSEIGRKLFISTKTASVHVSNILAKLSMTSRTQIAAWVVSEGVPH"
BASE COUNT          816 a         1501 c         1406 g          665 t
ORIGIN      
        1 ccgcgtgcac cgcggcttgc gcgagccgaa tgcagaagac ggcgtcgtac gcattcgccg
       61 gcacgctgcg aatagcgtag ccggggtcga cgtaccgcaa gctgagctcg gaacctgcgt
      121 cagcgaagga cgcgacaatg cgatcgcgca tgagctggcc gatgtcggcg agccgggtgt
      181 tgcccgaggc atcgcgagaa ccgtccagcg gcagcaggtg ctggccggcg ccctccgcaa
      241 ccacgacgac cgcgtgcccc tgatggtgaa cccgccgccg caggtggttc aagaagccgt
      301 catcaccctc gagctcgaac ggcacttcag gcacaagcac gaagtcgacg tcgtggttgg
      361 ccagcgcggc gtagcacgcg atgaagcccg agtgtcgacc catcagcttc accaggccga
      421 cgccgtgtgt cgtggagctc gcctcaatgt gtgcggcacg aatcgactcg gctgcccgcg
      481 cgaaagcggt ctggaacccg aaactgtgat cgaggtacgg gatgtcgttg tcgatcgtct
      541 tgggcacacc caccacagac agctcgtacc cgagcgcagc agccgcatca acaagcttct
      601 gcgcgccgcg caaagtgccg tcgccgccga ccacgaagag caccgagatg tcgagccgta
      661 tcaaggtggc gaccatctcg tgcgcatcct ggtcaccgcg agaggtgccc aggatcgtgc
      721 caccgcgatt gtgaatgtca tggacgacct ggggcgtgag agccatcggc gggtcggcac
      781 tgacaagccc acggtagccg ttgcgaaagc ccacgatgtc gatgacgccg tagttctgtg
      841 agagctcgag gaccaggccg cgaatgacgt tgttgaggcc cgggcacagg ccgccgcagg
      901 tcacgatgcc ggcgcgcgtg gtgcccggtt cgaagtagag ccgtgcacgc gggccgccag
      961 gctcgaacga cggcagctgc gctgcaggca aattgcgggc gaccgccatc tcgacggtgt
     1021 cgtcgagcaa cacccggtcg gactcgtgca cgtagtgcac agaactcgcc ctgtcgcgca
     1081 gcagccgcgt cataggcgag gtcaccgtcg tctcgcccag ccgcgtgacc atcaggtcat
     1141 cggcggtgat cgccagctga tcagcggtgc tcaccattcc gcgagcatgc cgcgcggacg
     1201 tttcgagcgt gtgtcggccc gagacgccgc cggaaccccc ggcgaacgca gaacggtact
     1261 cagacgaata ttaccgtaca aactatcttt tctcgactaa atcatctcat aataggtgag
     1321 tggactcaat agggtggatc gcgcggacgt cggcgctgac ggcggtactc gcgctgacag
     1381 gttcggtcgg ggtggggatt ggggcaggct cggccagtgc ggcgtccgtg ccgcgaccgg
     1441 cccatgtcgt cgtcgtgctg atggagaacc acgcctacgg ccagatcatc ggcagtacga
     1501 gcgctcctta cctgaactca ctcgcctacg ccggggcaag cttcaccaac gcccacggcg
     1561 tgacccatcc gagccagccg aactacctcg cgctcttctc cggcagcacc caaggcgtta
     1621 ccagcgacag ttgcccgcac acgttcccgg caggcaatct ggccagcgag ctcgtcgccg
     1681 ccaagctcgg cttcaaggcg tactcggaga gcttgcctgc cgccggctcg atggtgtgca
     1741 cgagtggctc gtatgcgcgc aagcacgcgc cgtggacgaa cttcaccgat gtgccagcca
     1801 gccgacagtc tcgccagctt tcccacgagt tacaccagcc tgccgaccgt gtcgtttgtc
     1861 gtcccgaatc tccaacacga catgcacgac ggaacaatcg cgcaggggga cacttggctg
     1921 aaagcgcacc tcggttcctg tgtcacatgg gcgaggacac acaacagctt gctcgttgtg
     1981 acctgggatg aagacgacaa cactgcagcc aacctagtcc cgacgatatt catcgggccg
     2041 atgatcaagg ccggtaagta tggtgaatcg atcaaccact acagaacatt gcgcacgatc
     2101 gaggacgcgt tcgggttgaa gcccctcgga acgtcagcgt cagtcgcacc catcactgac
     2161 atttggtgaa ggcgcaacca ggcttagtgc ggcacgccct ccgataccac ccacgcggcg
     2221 atctgcgtgc gtgaggtcat cgacaacttg gccaagatgt tcgatacgtg caccgacgcc
     2281 gtcttcgtcg agatgaagag cttgcggccg atctctgagt tgctaaggcc cgcggcgaca
     2341 agtgccgcca cctcgagttc acgcgcggtc aactgacccg aggccacccg agctcctccg
     2401 gacgcgagcc ggtgttgcaa cgatacgagt tcatcacgac gccacccggt ccaccgagca
     2461 aggaatacct cggcttcggc tgcggcagtc tgcgcctctt cgatgttgcc gagcacgagc
     2521 aggctgcggg ccaggccgat ccggacgtcg gcatcgacaa tcgcgcccgg acgccggcgc
     2581 ggctcggcaa gtgcggaccg gtatagctcg atagcagctc cgtgctcgcc ggatgcggcc
     2641 gccaacgcag cttgcacgtg cagcacccat ccgacatcca catcgcgaat cgtgcggttc
     2701 gggctgatga aaagaagctc gagcacgcgc ccgacctcgg ccggagcaag cccggcccgc
     2761 accgcagcga gcagctcgac gccccaggtc caggacgaca gattgcagac acccgttggc
     2821 gcggcaatcg cccgtcggcc caggtcggcg agtcggcgtg cgaccgtcgc gacatcaccg
     2881 actcgagctg cggcctccat gctcagcgtc tcgacccagt ggtcttgcgc ggtgctcgta
     2941 ccgccaacag ttaacggagc cagcgcaaac atcgcgctcg ccgccgccat ggcatcgtcg
     3001 tcgccgagtt cgcccgcaag ccaggcgcgc tcggcgaaca gccacccgcg cttgccgccc
     3061 gcgtcggccg tcaagtcgat cagggcatcg tcgagcacag cgcgtgctgc cagcggatcg
     3121 ccgagcacgt tcgagtgata agccgccgtg attcccgcaa ggccggacag ccagtcgtgc
     3181 cggccgaaac tggaaaccaa ctcggtcatc cgcgaaacat gcgaccacac ccgatccgca
     3241 ggccaccgca cgagcacgcc ctgcaacagg ttgttcaacc cccgcagcgc gctgaagacg
     3301 tcgccggcag cgactgcggc gtcgatggcg gcaacaagca atgccgcacc ttcatcgaac
     3361 cggccgggga tgtcgaaaac cgccgacccc ttgttcacca tcgctgccgc gcgggtctcg
     3421 tcgtcgtccg ccatcagcgc aagcgcccgg tcggcccact cgacggcttc ggcggattgg
     3481 cccgcgagca tccgcacctc ggcgacgtag ctgaacacgg ccgcgaggtc cggaccgtcg
     3541 ggcagccggt cggcgacact cagcaatcga gtaacgagag cagcgtggtc gtcggtgtcg
     3601 ccgacttccc atttgagccg cgcgagtgcg cgcaacgccc ggctgagctc accgtcgttg
     3661 tcaccggctt cggcgagtgc cgcccactgg gtcgcgtaat tcgcggcatt ggcgaggagg
     3721 ctgcaagccc acgcggcgcg cgtcgcgagc tcgagcagga cggcgtctgc gtcgacctcc
     3781 tggagaccga gctcggcaag tcgcagcgcc tcgtaagtcg acccggtgcg caggtgcacc
     3841 gcggcgccgc ggcgggcggc gtcgacaacg tcgtcccaca agtgcgcacc cgccgcgtga
     3901 tacgcgagag ctccccagtc gtcgctaccc atctccagca acgccgcgtg cgccttctcg
     3961 tggagccgac ggcgttgccg cccgagcagg cggctcgcga ctgcttcacg ggtgagcgcg
     4021 tggcggaagc tgaagacgtc cggctcgacc tcggtgacca ggccgcgttc aacgaggtca
     4081 cgcatcgtcg cgatcaggtc atcctcgccc gcgccggaca cgatcgcgag caggtcgaac
     4141 gggatccggc ggccgagcac ggcggccgag tcgacgatcc gccgctgggt gtcgggaagt
     4201 ccgtcgaggt gacgcagcac ggcctcgctc acgctgatcg gcagcggcag cgcacacagc
     4261 gcctcgagtt cgacaccggg ggctgagctg accagttcct cgaggaagaa cgggtttccc
     4321 gaggtgcgtc gcgccagatc ggtggccact cgccacggaa cccggctctg gtagacggcg
     4381 gcgagcca
//
LOCUS       DATCBJ010000523         3553 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_177929, whole genome shotgun sequence.
ACCESSION   DATCBJ010000523 DATCBJ010000000
VERSION     DATCBJ010000523.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 3553)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3553)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3553
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_177929"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1223)
                     /gene="miaB"
                     /locus_tag="VII50_10590"
     CDS             complement(<1..1223)
                     /gene="miaB"
                     /locus_tag="VII50_10590"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HEY5051339.1"
                     /translation="MSTSQQQPPRTYQVRTYGCQMNVHDSERLAGLLEVSGYIKADAD
                     ELADVVVFNTCAVRENADNKLYGNLGQLYSSKLLKPSMQIAVGGCLAQKDRGEIVRRA
                     PWVDVVFGTHNVGSLPVLLERARVADEAQVEILESLEVFPSTLPSRRESVYAAWVAIS
                     VGCNNTCTFCIVPSLRGTEKDRRPGDILLEIETLVSDGVIEVTLLGQNVNSYGVEFGD
                     PLAFSKLLRACGLIDGLERVRFTSPHPHDFTDDVIDAMATTHNVMPQLHMPLQSGSDD
                     VLRRMRRSYRREKYLGIIDRVRAAMPDAAITTDIIIGFPGETDEDFEHTLDVVKRARF
                     ANCYTFQYSKRPGTPAADMDAQVPPSVVRQRYERLVAVADEVAWDEAKTQVGRVVDVL
                     VAEGEGRKDDQTHRM"
     gene            1291..3267
                     /locus_tag="VII50_10595"
     CDS             1291..3267
                     /locus_tag="VII50_10595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HEY5051340.1"
                     /translation="MSETPQVLPYGSWPSPITAADVAKGAIRLGFPALVETANPGDAP
                     DVWWLEGRPAEGGRQVIVSGERGDLLPAPWNARTRVHEYGGLSFLPLADGLLFTEWSD
                     QRLYYLASADASEPRPLTPVPAHPAALRYADPVLSPDGREVWCVREQHADPSAPSAGI
                     TRAIVGVPLDGSAAVDETVVRIIVSGSQFLAFPTPSPDGGKLAWIAWDHPNMPWDTTK
                     LRVGELTNGIVQSWRTVLGGQHESVLEPAWANTDRLYAISDRSGWWNLYRVDLTGAAE
                     PLAPRDEEFAGPLWQLGFRHYGALDDGKLIVLHGDADQKLGILDPATSVIDDFELPFT
                     HWQPAISHRGRYLAGVAGSAVAPNAVVRVDLQTGEHVVLRWAADDVPADDYLPRPYPA
                     TFEGPGGRDVHAHVSAPRNPHAQSPAGELPPFIVFVHGGPTSSSPPLLALEHAYFTSR
                     GIGVIDVDYGGSSGYGREYRERLRGQWGIVDVEDCVAAAQALAKAGEADAARLAIRGG
                     SAGGWTTLAALTQTHAFGAGTSYFGVAELLRFVNDTHDFESRYLDGLVGPLPEAHDLY
                     VERAPLSHVDEVSCPVLLLQGLDDHIVPPSQAEMFRDALVAKGIRHAYLAFEGEQHGF
                     RKAENVIASLEAELSFYGQVFGFTPPGVPVLELS"
     gene            complement(3274..>3553)
                     /locus_tag="VII50_10600"
     CDS             complement(3274..>3553)
                     /locus_tag="VII50_10600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5051341.1"
                     /translation="PVLLHIASETIADLDPELGASLLGDRAHSVIFDNSKVKSLVPDY
                     VAAIPFATGARQIVDWYDADPARQVIDAGLNATFDKLVAASRFGKLPA"
BASE COUNT          579 a         1215 c         1137 g          622 t
ORIGIN      
        1 ctcatgcgat gcgtctggtc gtccttgcgt ccctcacctt cggcgacgag aacgtcgacg
       61 acgcgcccga cctgggtctt cgcttcgtcc cacgcgacct cgtcggcgac ggcgaccagc
      121 cgctcgtacc gctgacgcac aaccgacggt ggaacctgag cgtccatgtc tgccgccggc
      181 gtgccaggac gcttggagta ctggaacgtg tagcagttcg cgaatcgcgc ccgcttcacg
      241 acgtccaacg tgtgttcgaa gtcttcatca gtctcaccgg gaaagccgat gatgatgtcg
      301 gtcgtgattg ccgcgtcggg catcgccgca cgcacgcgat cgatgatgcc gaggtacttc
      361 tcgcggcggt acgagcggcg catccgccgc aagacatcat cagagcctga ctgcaagggc
      421 atgtgcaact gcggcatgac gttgtgcgta gtcgccatcg cgtcgatcac gtcgtccgtg
      481 aaatcgtgcg gatgcggcga ggtgaaccgc acgcgttcga gaccgtcgat caaaccgcac
      541 gcacgcagca gcttcgaaaa tgccagcgga tcgccgaact cgacgccgta cgagttcacg
      601 ttctgaccca gcaacgtgac ctcgatgaca ccgtcgctga ctagcgtctc gatctccagg
      661 agaatgtcgc ccggccgccg gtccttctcc gttcctcgaa ggctcggcac gatgcagaac
      721 gtgcaggtgt tattgcagcc gacactgatg gctacccacg ctgcgtagac cgactcgcgt
      781 cgcgacggca gcgtcgacgg gaaaacctcg agcgactcga ggatctcgac ctgcgcctcg
      841 tcggccaccc gagcccgttc gagcagcacc ggcaacgacc cgacattgtg ggtgccgaac
      901 acgacgtcga cccacggcgc cctgcgaacg atctccccgc ggtccttctg cgccaggcaa
      961 cctccgacgg cgatctgcat gctcggcttc agcagcttgg acgagtaaag ctgcccgagg
     1021 ttgccgtaga gcttgttgtc ggcgttctcc cgcaccgcac acgtgttgaa cacgacgaca
     1081 tccgccagct cgtcggcgtc cgccttgatg tacccggaca cctcgagcag acccgccagt
     1141 cgctctgaat cgtgcacatt catctggcag ccgtaggtgc gcacctgata cgtgcgcggc
     1201 ggctgctgtt gcgacgtgga cacctgatca gggtacgcag ggtctcgccc gttctggcgg
     1261 cccatcgtgc ggcactgcca gaatgagccg atgagcgaaa caccgcaggt cctgccctac
     1321 ggctcctggc cctcaccgat cacggccgcg gacgtcgcca agggcgccat ccggctcgga
     1381 ttccccgccc tcgtcgagac cgcgaatccg ggcgacgcgc ccgatgtgtg gtggctcgag
     1441 ggccgaccgg ccgagggtgg ccgccaggtc atcgtgtcgg gcgagcgcgg tgacctgctg
     1501 cccgctccgt ggaacgcgcg cacccgagtg cacgagtacg gcggcttgag tttcctgccg
     1561 ctggccgacg gcctgctgtt caccgagtgg tcggatcaac gcctctacta cctcgcgagt
     1621 gcagacgcct ccgagccgcg acccctgaca ccagtcccgg cgcatccggc cgcgttgcgc
     1681 tacgccgacc cagtgctgtc gcccgatggc cgcgaggtgt ggtgcgtgcg cgagcagcac
     1741 gcggacccat cggcgccctc tgcaggaatc acgcgcgcga tcgtcggcgt cccgctcgac
     1801 ggctctgcgg ccgttgacga gaccgtggtg cgcatcatcg tgtcgggaag ccaattcctg
     1861 gctttcccca cgccttcacc ggacggcggg aagctcgcct ggatcgcatg ggaccacccg
     1921 aacatgccat gggacacgac caagctgcgc gtcggcgagt tgaccaacgg gatcgtgcag
     1981 tcgtggcgca cggtcttggg cggtcaacac gaatccgtcc tcgaaccggc ctgggcgaac
     2041 acggatcgcc tctatgcgat cagcgaccgg tcgggctggt ggaacctcta ccgggtcgac
     2101 ctcaccggcg cggcggagcc gttggcgccg cgcgacgaag agttcgccgg cccactgtgg
     2161 cagctcgggt tccggcacta tggcgcactc gacgacggca agctgatcgt cctgcatggc
     2221 gatgccgacc aaaagctcgg catcctcgat cccgcgacca gcgtgatcga tgatttcgag
     2281 ttgcccttca cccattggca accggcgatc tcgcatcgag gtcgctacct ggccggcgtc
     2341 gccggcagcg ccgtcgcgcc gaacgccgtc gtccgcgtcg acctgcagac cggcgagcac
     2401 gtcgtgctgc ggtgggcggc tgacgacgtg ccagctgacg attacctgcc acgtccgtac
     2461 ccggcgacct tcgagggccc gggcggccgc gatgtgcatg cgcacgtctc cgcgccccgc
     2521 aatccgcacg cacagtcccc tgcaggcgaa ctgccaccgt tcatcgtgtt cgtgcacggt
     2581 ggcccgacgt cgtcatcgcc gccactgctc gctctcgaac atgcgtactt caccagtcga
     2641 ggcatcggcg tgatcgacgt cgactacggc ggctcgtccg ggtacggccg ggagtaccgc
     2701 gagcgactgc gcggccagtg gggcatcgtc gacgtcgagg attgcgtggc cgcggcccag
     2761 gcgctcgcga aggcgggcga ggcggacgcc gcgcggctgg ccattcgcgg cggcagcgcc
     2821 ggcggttgga ccactctggc cgcgctcacc caaacacacg cgttcggagc cggcacctcg
     2881 tatttcggcg ttgcggagtt gctgcgtttc gtcaacgaca ctcacgactt cgagtctcgc
     2941 tatctcgacg gtcttgtcgg gccgctgccg gaggcgcatg acctctacgt cgaacgtgcg
     3001 ccgttgtcac acgtcgacga ggtctcgtgt ccggtgctgt tgctccaggg tctcgatgac
     3061 catatcgtgc cgccttcgca agcggagatg ttccgcgatg ccctggtagc aaagggaatt
     3121 cgccatgcct atctcgcgtt cgaaggcgag cagcacggct ttcgcaaggc cgagaacgtg
     3181 atcgcgagcc tcgaggccga gctgtcgttc tacggccagg tgttcgggtt caccccgcca
     3241 ggtgtgcccg ttctcgaact gtcgtagcgc cggctatgcg ggcagctttc cgaacctcga
     3301 cgctgccacg agcttgtcga atgtcgcgtt cagaccggca tcgatgacct gtctggccgg
     3361 gtcggcgtcg taccaatcga cgatctggcg agcgcccgtg gcgaacggaa tcgcggcgac
     3421 gtagtcgggc actagcgact tcaccttgct attgtcgaag atcaccgaat gcgctcgatc
     3481 gccgagcagc gatgcgccaa gctctggatc gaggtccgcg atcgtttccg aggcgatgtg
     3541 cagcaggacg ggt
//
LOCUS       DATCBJ010000524         3390 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_294444, whole genome shotgun sequence.
ACCESSION   DATCBJ010000524 DATCBJ010000000
VERSION     DATCBJ010000524.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 3390)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3390)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3390
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_294444"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..906)
                     /locus_tag="VII50_10605"
     CDS             complement(<1..906)
                     /locus_tag="VII50_10605"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008964 - phosphoenolpyruvate carboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0015977 - carbon fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HEY5051342.1"
                     /translation="MSVPQSANESGSLQPEPVARERARLEMPEPLRRDVRLLGELLGQ
                     VLREYGGDDLLADVEELRHAVIAARTGAGPPESVVALVRGWSLDRAEEVARAFTVYFH
                     LANLAEEHHRVRVLRSRDAGEGRAGDSLATSVAEVRRLHSEERVASIVDGLLLHPVLT
                     AHPTEARRRAVVTAISRAGSQLDRIDDPRAGAGEQTDARRRLLEEIDILWRTAHLRAT
                     AVDPLDEVRTAMAVFDETLFRVVPTLYRGLDHVLLGDRSGTVTPAAHPFFRLGSWIGG
                     DRDGNPNVTAQITRDALGIQSGHVLL"
     gene            965..2545
                     /locus_tag="VII50_10610"
     CDS             965..2545
                     /locus_tag="VII50_10610"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020662569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HEY5051343.1"
                     /translation="MSVDGVDPHTSAGKLADLLGRDAEAVHAGTAAAVERQHAKGKGT
                     ARERIDALLDPDSFVELDRLARHRSTAFDQAASRPYGDGVIAGYGTIDGRQVCVFSQD
                     FTVFGGSLGEVFGEKIVKIMDLAMKVGCPVIGINDSGGARIQEGVVSLGLYAEIFYRN
                     TIASGVIPQVSLVLGPCAGGAVYSPAITDFIIMVDKTSHMFITGPDVIKTVTGEDVEF
                     EELGGAHTHNARSGVAHYLATDEADAIDFAKALLSYFPSNNVDDAPVFPAETSFEVSA
                     TDLALDTLVPDAANQPYDIHTVIKAVLDDAEFLETQALFAPNIVCGLGRVDGRSVGVV
                     ANQPMHYAGCLDIDASEKAARFVRTCDAFNIPILTFVDVPGFLPGTGQEWNGIIRRGA
                     KLLFAYAEATVPKVTVITRKAYGGAYDVMGSKHLRADVNLAWPTAQIAVMGAQGAVNI
                     LYRKELAASASPERDRADYVAQYEDHLANPYIAAERGYVDAVIAPSQTRLEVTRALRI
                     LRTKRDQLPPKKHGNIPL"
     gene            complement(2824..>3390)
                     /locus_tag="VII50_10615"
     CDS             complement(2824..>3390)
                     /locus_tag="VII50_10615"
                     /inference="COORDINATES: protein motif:HMM:NF017280.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylxylan esterase"
                     /protein_id="HEY5051344.1"
                     /translation="AGSPAEPGFMTRGIGSAESYYYRRVFVDAVRAVDAARSHPLVDP
                     ARVAVAGGSQGGGITIAAAALCDGLVAAMPDVPFLCDFRRAVRVTDGDPYGELVRYLH
                     THRDRIEQVFGVLSYFDGASLAARASASGLFSVALRDPICPPSTVFAAYNAYAGPKRI
                     EVYDFNEHEGGGPFHQRVQFDFAREVFA"
BASE COUNT          609 a         1124 c         1132 g          525 t
ORIGIN      
        1 cagcagcacg tgacctgact ggatccccaa cgcgtcgcgg gtgatctgtg cggtcacatt
       61 cgggtttccg tcgcggtcgc cgccgatcca gctgccgagg cgaaagaacg gatgggcggc
      121 gggggtcacg gtgcccgagc ggtcgccgag cagcacgtgg tcgagtccgc gataaagagt
      181 ggggacgacg cggaacagcg tctcgtcgaa gacggccatc gcggtgcgca cctcgtcgag
      241 cgggtcgacg gcggtggcgc gcaaatgcgc ggtgcgccag aggatgtcga tctcctcgag
      301 cagccttctg cgtgcgtcgg tttgctcccc cgcaccggca cgtgggtcgt ctatccgatc
      361 gagctgcgaa ccagcccgcg agatcgcggt cacaacggca cgacggcgag cttctgtcgg
      421 gtgcgcggtc aacaccgggt ggagcaaaag gccatcgacg atgctggcca cccgttcttc
      481 actgtgcagg cggcggacct cggccaccga cgttgccagc gagtcacctg cgcggccctc
      541 ccccgcgtcg cgcgaacgta agacgcggac tcggtggtgt tcctcggcga ggttggccag
      601 gtggaagtag accgtgaacg cgcgagcaac ctcttcggcc cggtcgagcg accagccgcg
      661 cacgagggcc accacgcttt cgggaggccc cgcccccgtc cgcgcggcga tgaccgcgtg
      721 cctcagctcc tcgacgtccg ccagcagatc gtcgccgccg tattcgcgca agacctggcc
      781 gagcagctcg ccgagcaatc ggacgtcgcg gcgcaagggc tcaggcatct caagccgggc
      841 gcgctcccgg gcgacgggct ccggctgcag cgagcccgac tcgttcgcag actgcggcac
      901 cgacacgggc acagcgtact ggcggcgtac tgtcgcccga gacggaccag ctgaggagga
      961 ctgcatgagc gtcgacgggg tcgacccgca caccagcgcc ggcaagctcg cggacctgct
     1021 aggccgggac gccgaggccg tgcatgcagg aacggcggcc gctgtcgagc gccagcatgc
     1081 gaagggcaaa ggaaccgcgc gggaacgcat cgatgcgctt ctcgaccccg actcgttcgt
     1141 cgaactcgat cgcctcgcgc ggcatcggtc gacggcgttc gaccaggcgg ccagccgtcc
     1201 gtacggcgac ggggtcatcg ccggctacgg caccatcgac ggccgtcagg tctgcgtgtt
     1261 ctcccaggac ttcaccgtgt tcggcggcag cctcggcgag gtgttcggcg agaagatcgt
     1321 gaagatcatg gatctcgcga tgaaggtcgg ttgtccagtc attggcatca acgactcggg
     1381 cggcgcacgc attcaagaag gggtcgtgtc gctcggtctg tacgccgaga tcttctatcg
     1441 gaacacgatc gcgtccggag tgatcccgca ggtttcgttg gtgctcgggc catgtgcggg
     1501 cggcgccgtg tactcaccgg ccatcaccga cttcatcatc atggtcgaca agacgtcgca
     1561 catgttcatc actggtcctg acgtcatcaa gaccgtcacc ggcgaggacg tcgagttcga
     1621 agaactcggt ggcgcgcaca cgcacaacgc gcgatcgggt gtcgcgcact acctggcgac
     1681 ggacgaggcc gacgcgatcg acttcgcgaa agccctgctg tcgtacttcc cgtcgaacaa
     1741 tgtcgacgac gcgccggtgt tcccggccga gacgtcgttc gaggtgagcg cgaccgacct
     1801 cgcgctcgac acgctcgtcc ccgacgccgc caaccagccg tacgacatcc acacggtgat
     1861 caaggcagtc cttgacgacg ccgagttcct cgagacgcag gcgctgtttg ccccaaacat
     1921 cgtctgcggg ctcggtcggg tcgacggcag atcggtcggg gtggtcgcca accagccgat
     1981 gcactacgcc gggtgtctcg acatcgatgc atcggagaag gccgcgcgct tcgtgcgcac
     2041 ctgcgatgcg ttcaacatcc cgatcctcac gttcgtcgac gtccccggct tcttgcccgg
     2101 cacgggacag gagtggaacg gcattatccg gcgcggcgcg aaactgttgt tcgcgtacgc
     2161 cgaggcgacg gttcccaagg tgacagttat cacccgcaag gcgtacggcg gtgcctacga
     2221 cgtcatggga agcaaacatc tgcgtgccga cgtgaacctc gcgtggccga ccgcgcagat
     2281 cgcggtcatg ggagcgcaag gtgccgtgaa catcttgtac cgcaaggaat tggcggcgtc
     2341 ggcttcacct gagcgcgatc gggctgacta cgtcgcacag tacgaggacc atctcgcgaa
     2401 cccgtacata gcggctgaac gcggctacgt cgacgccgtg atcgcgccgt cgcagaccag
     2461 gctcgaggtc acccgcgcgc tgcggatcct gcgcaccaag cgcgaccagt tgccgccgaa
     2521 aaagcacggg aacattccgc tgtgaacgag cccctccagg ttacgaaggg cagcccggac
     2581 gacgaggagc tggccgccgt tgtcatggcc ctcgaggtgg tcgccgcgcg cccgctggtt
     2641 tctgctccga ctccagtctc gaagtggaga acatcactgg gcgcagtttc gatgaacgca
     2701 atcgggttcc gcgccgagtg gggacaaacg cgcccctaac ctcgggcggc gtgtcgcgtg
     2761 catatgtccc cactcggtgc aggaacagag catgggtgct cggccagcta cgcgctcggg
     2821 aggttaggca aatacttcgc gagcgaagtc gaattggacg cgttggtgga atggcccgcc
     2881 accctcgtgc tcgttgaagt cgtagacctc gatccgcttc gggccagcgt aggcgttgta
     2941 ggccgcgaac accgtcgagg gcgggcagat cggatcgcgt aacgcgacag agaacagtcc
     3001 ggacgccgaa gcccgagccg ccagagacgc gccgtcgaaa tatgacagga caccgaagac
     3061 ctgctcgatg cgatcgcggt gcgtgtgcag gtatcgcacc aactcgccgt acgggtcacc
     3121 gtcggtcacc cgcactgcac ggcggaagtc gcacaggaac ggcacgtcgg gcatcgccgc
     3181 gaccagacca tcgcacaagg ctgcggccgc gatcgtgata ccgccgccct gactaccgcc
     3241 agctaccgca acccgagcgg gatccaccaa gggatgggag cgggcagcgt cgacggcccg
     3301 cactgcgtcg acgaacaccc ggcggtagta gtagctctcc gccgacccga taccgcgtgt
     3361 catgaacccg ggttcggctg gcgaaccggc
//
LOCUS       DATCBJ010000525         5407 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_284266, whole genome shotgun sequence.
ACCESSION   DATCBJ010000525 DATCBJ010000000
VERSION     DATCBJ010000525.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 5407)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5407)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5407
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_284266"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..651
                     /locus_tag="VII50_10620"
     CDS             <1..651
                     /locus_tag="VII50_10620"
                     /inference="COORDINATES: protein motif:HMM:NF014667.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HEY5051345.1"
                     /translation="NQPLPSSVTSIQFTDDTGKQLTLAAFTGKDVVFTPFMTLCQETC
                     PLTTGNLQRLDTSVINAHQEDNVQMVMITIDPSRDTVARLHAYRAVAQITQPNWTLLT
                     ATPADLTQMWKSLGVDVEKTAEDNPPGIDWMTGQPLTYDLSHTDEVFMLNKSGNERFL
                     IDGMPNTQGVAPAPILDKFLSARGRDNLAHPDASTWTVADATTSLAWVLGKHLPKP"
     gene            complement(810..2315)
                     /locus_tag="VII50_10625"
     CDS             complement(810..2315)
                     /locus_tag="VII50_10625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HEY5051346.1"
                     /translation="MGATQLDLTLLLGAVVLLAAILAVRFATRIGLPGLLVYLAIGLA
                     LQSIVHFDDARRAQSLGLAALVLILAEGGLTTRWDDVRPAVVPAVLLSTFGVAISMSV
                     TALIAHLVLGGSWRLAFLLGAIVSSTDAAAVFSMLRRLKLPTRLAGVLEVESGLNDAP
                     AVIAVVLLSSHHPQTNPGIVLALVCYELVAGAAIGFGVARLGVLALRRLALPASGLYP
                     LSILALAVAGFATAALAHASGFLAVYVAGLMLGNARLPHRPATRSFAEGIAWLAQISL
                     FVMLGVLVIPSALPREILPAIAVGSALLLLARPVSVALSLLPLGIRRVRVEAFGRAER
                     LFLSWAGLRGAVPIVLATVPVTANVPRSRELFGLVFVLVVVFTLVQGPTLPLVARLLG
                     LVVAAEPSHIDVESAPLEDLDAEVLQLRIPPGSLLHGVEIFELRLPRGAAVTLVVRDG
                     SSFVPTDETELRRGDALLIVTTASARVEAERRLRAVSRRGKLAAWYGDRGE"
     gene            complement(2322..2909)
                     /locus_tag="VII50_10630"
     CDS             complement(2322..2909)
                     /locus_tag="VII50_10630"
                     /inference="COORDINATES: protein motif:HMM:NF013934.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HEY5051347.1"
                     /translation="MNCPDFALAKTSLRRQLLVRRGQHTDAHRAQTATALARAFAAEF
                     EPRVRGEPTGTAAPLRVVAVYLSFATEPDTGPLIEWLADQRVRALAPTLLPDADLGWM
                     DVPDGPPLGLDAITDAEVILVPALAVDLSGHRLGRGGGSYDRALTRVRPRQTVLAVVH
                     DDEVLDAVPFEPHDRIVDGVLTPSGVRYFGTAADG"
     gene            3088..5193
                     /locus_tag="VII50_10635"
     CDS             3088..5193
                     /locus_tag="VII50_10635"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HEY5051348.1"
                     /translation="MALRTRLSLAFVLIVLVPILVGAIAVLIAVPQVLKSQIATRLRT
                     AGVSVTDVLSARCMQAQQAAQLLGLEVATLGPQAAVTHLVSDRAVDYAVVSGSDGAIV
                     ASAGQLAGAPAQVAPSVLNSCGDAKNANIAISSRAGLAIAESPQLSQVSVAYSVGSTT
                     AASLRSALSGSTAVTLTTGGALVSSTLTGTQAAKQTAAAKAQVADVAQSPRVFDNAGR
                     ILAFVPPSANQPYGVIVSEPVPDTTGLTLVLAAIVLVTILAGLVMGRLLARLISGPVV
                     ELSEAAARAASGDLDIAIPVRSKDEVGQLATAFNHMTSELQTYIRQLERSRDELRQNL
                     DRLGATLTHTHDLDGILAVVLDTAIGSAQATGGAIMFLDADGNLTVQVRRGEVADSVD
                     VGTIIPLGKGISGQVAVTGEPVCGHAGEGPGLRRVPSEPDAASVIAVPLKQSGRIVGV
                     LNLYDKEGAREFTPHDLDTILAFSTQAGVAIDNVLLHQEAQRLSLTDPLTGLWNYRYL
                     TIGLGHEIERATRFSRPLAALMLDLDRFKSINDKYGHQVGDAVLIELAGRMRAEVREV
                     DTVARYGGEEFVIVLPETDAIGAARLATRLGTVIRSTPFCLDLGSNGHAVDPHKGLQV
                     TASIGAAVFPEHGMTPSRLLRSADDALYAAKDAGRDGWRFGLATEDDGTNEQRRVLVV
                     PDTDIALPKSADRPRFPDD"
     gene            5258..>5407
                     /locus_tag="VII50_10640"
     CDS             5258..>5407
                     /locus_tag="VII50_10640"
                     /inference="COORDINATES: protein motif:HMM:NF012695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HEY5051349.1"
                     /translation="MADQKVTKAVIPAAGLGTRFLPATKATPKEMLPVVDKPAIQYVV
                     EEAVAA"
BASE COUNT          997 a         1851 c         1767 g          792 t
ORIGIN      
        1 aatcagccgc tgccgtcgtc ggtgacgtcg atccagttca ccgacgacac cgggaagcag
       61 ctgacactgg ccgcgttcac gggaaaggac gtggtgttca cgcctttcat gacgctgtgc
      121 caggagacgt gtccgctcac gacgggtaac ttgcagcggc tcgacacgtc ggtcatcaac
      181 gctcaccaag aagacaacgt gcagatggtg atgatcacca tcgacccttc gcgcgacaca
      241 gtcgcccggc tgcacgccta ccgggcggtc gcgcagatca ctcaaccgaa ttggacgctc
      301 ttgacggcca cacctgccga cctcacgcag atgtggaagt cgctaggtgt cgacgtcgag
      361 aagacggcag aggacaaccc tcccggcatc gactggatga ccggtcagcc gctcacctac
      421 gacctcagcc acaccgacga ggtgttcatg ctcaacaagt cggggaacga gcggttcctg
      481 atcgatggaa tgccgaacac gcagggcgtt gcgccagcac cgattctcga caagttcctc
      541 agtgcccggg gtcgcgacaa cctcgcgcat cccgacgcct cgacctggac tgtggctgac
      601 gcgacaacct ccctcgcctg ggtgctcggc aagcacctcc cgaagccgta atgcggcgct
      661 tagcagactg cggaagacct cttctctggc agctttgctc ggccgctaca tgactgcaga
      721 gcaaagcgcg cgaacgagtg ggctgcgcgt cgcgagactc ccgggaccgc gctggccgcg
      781 aaagccctcg aagctgccgc caacgcctgt cactcgcccc ggtctccgta ccaggcggcg
      841 agtttgccgc ggcggctgac tgctcgcagg cggcgttcgg cttcgactcg tgcggacgcg
      901 gtcgtcacga tcagcagcgc gtcgcctcgg cgcagctctg tctcgtcggt tggcacaaag
      961 ctcgatccat cgcggacgac gagggtgacg gccgccccgc gcggcagccg caactcgaag
     1021 atttctactc cgtgcagcag cgacccgggg gggatgcgca gttgcagcac ctcggcgtcg
     1081 agatcctcca gtggcgcgga ttcaacgtcg atgtgcgacg gctcggccgc gaccaccaac
     1141 cccaggaggc gggcgaccaa tggcagcgtc ggaccctgca cgagggtgaa cacgacgacg
     1201 agcacgaaca ccagcccgaa caactcacgg ctacgaggaa catttgcggt cacggggacg
     1261 gtggccagca caatcggtac ggcaccccgc agtcccgccc acgaaagaaa cagccgctca
     1321 gcgcgaccaa acgcctcgac gcgaacccgc cgaattccca gcggcaacag cgaaagagcg
     1381 accgaaacag gtcgcgcgag caacagcaaa gctgaaccga cggcgatcgc aggcaggatc
     1441 tcgcggggga gcgcggacgg aatcaccagc actccgagca tcacgaacag cgagatctgc
     1501 gccagccacg cgattccttc agcgaagctg cgggtcgccg gccgatgcgg cagccgcgcg
     1561 ttgccgagca tcagacccgc cacgtacacc gcaaggaatc cagacgcgtg tgcgagtgcc
     1621 gccgtcgcga acccggcgac cgcgagcgcc agtatcgaca acgggtacag gccggacgcc
     1681 ggcaacgcca gccgccgaag cgccaaaact cccagccggg ccacgccgaa accgatcgcc
     1741 gcgccggcga ccagctcgta gcacaccaac gcaaggacga tgcccgggtt cgtttgcggg
     1801 tggtgcgagc tgagcaacac gacggcaatg actgcgggcg cgtcgttcag cccggactca
     1861 acctcgagca ctccagcgag tcgggtaggc agtttgagcc tgcgcagcat ggaaaaaacg
     1921 gccgctgcat cggtcgacga cacgatcgcg ccgagcagga acgcgaggcg ccaactgccg
     1981 cccagcacga gatgcgcgat gagcgcggtc acgctcatgc tgatcgcgac cccgaaggtg
     2041 gacagcagca ctgcagggac gacggccggc cgcacgtcgt cccatctcgt tgtgagtcca
     2101 ccctcggcga ggatcagcac gagcgccgcg agcccaagag attgcgcgcg tctcgcgtcg
     2161 tcgaaatgca cgatgctttg cagcgcgaga ccgatcgcca ggtagacgag caggccgggc
     2221 agtccgatgc gggtcgcgaa ccggaccgcg aggatcgcgg cgagcagtac gacagcgcca
     2281 agaagcagcg tcaggtcgag ctgggtcgcg cccacgaaaa gtcagccgtc cgcggcggta
     2341 ccgaagtacc gcaccccgct cggagtcagc acgccgtcga cgatgcggtc gtgcggttcg
     2401 aacggcaccg catcgagaac ctcgtcatcg tggacgacgg cgagcacggt ctgtcgcggc
     2461 cgcacccgag tcaacgccct gtcgtaggag ccgccgccgc ggccgagccg gtgtccgctc
     2521 agatcgaccg cgagtgcagg aaccaggatc acctcggcgt cggtgatcgc gtcgagtccc
     2581 aacgggggac cgtccggcac gtccatccag ccgagatccg catcgggcag caaggtcggc
     2641 gcaagtgccc gcacccgctg gtcggcgagc cactcgatca gcggaccggt atccggctcg
     2701 gtcgcgaacg agaggtagac cgcgaccact cgcagaggcg ccgcggtgcc agtgggctcg
     2761 ccacgcaccc ttggctcgaa ttccgccgca aaggcccgcg cgagggcggt agcggtttgc
     2821 gcccggtggg cgtcggtgtg ctgccctcgc cggaccagca gctgtcggcg cagggacgtt
     2881 ttggcgagag cgaagtcggg acaattcatc accaatctcc cttcgtgtcc cttttaaccc
     2941 tctatgtggc ggcagagtaa cgacttatcc cccggctgga ggcttcgcag aggcgggcgc
     3001 ccatgcgagc atcgcatggg gagttacgcc gcttcgttat agatgtggtc acctgaccgt
     3061 ggcgacgaac accggaggtg ccggcagatg gcgttgcgca cccggctctc ccttgccttc
     3121 gtgctgatcg tcctggtccc gatcctggtc ggcgccatcg ccgtcctgat cgccgtcccg
     3181 caagtcttga aaagccagat cgccacccgg ctgcgcaccg cgggcgtcag cgtcaccgac
     3241 gtgctctcgg ccaggtgcat gcaggcgcag caggcggcgc agctgctcgg actcgaggtg
     3301 gccaccctcg gcccgcaggc cgccgtcacc cacctggtca gcgaccgcgc cgtcgactac
     3361 gcggtcgtat cagggtcgga cggcgcgatt gtcgcgagcg ctggtcaact tgccggcgcg
     3421 cccgcgcagg tagcgccttc cgttctcaac agctgcgggg acgccaagaa cgcgaacatc
     3481 gcgatctcca gccgcgcggg cctggcgatc gctgagagcc cgcaactgag ccaagtctcg
     3541 gtcgcgtatt cggtagggtc gacgacggcg gccagtcttc ggtcggcgct gtcgggttcg
     3601 accgcggtga cgctcacgac cggcggtgcg ctggtctcaa gcaccctgac gggcacgcag
     3661 gcagcgaagc agacggccgc ggcgaaagct caagtggccg acgtcgcgca gagcccaaga
     3721 gtctttgaca atgcgggccg catcctcgcg ttcgtgccgc cgtccgcgaa ccagccctac
     3781 ggcgtcatcg tcagcgagcc ggtgcccgac acaacgggcc tcacgctggt gctggccgcg
     3841 atcgtgctcg tgacgatcct ggccggactc gtcatgggcc ggctgctcgc gcgactcatc
     3901 tccggcccgg tcgtcgaact gtccgaagct gcggcacggg ccgcgagcgg tgacctcgac
     3961 attgcgatcc cggtgcgctc gaaggacgaa gtcggccaac tggcgaccgc cttcaaccac
     4021 atgacgagtg agttgcagac ctacatccgt caactcgagc gcagccgtga tgagttgcgg
     4081 caaaacctcg accggctcgg cgcgacgctc acgcacacgc atgatctgga cggcattttg
     4141 gcggtcgtcc tcgacaccgc gatcggcagc gctcaggcga ccggcggcgc gatcatgttc
     4201 ctcgacgccg acggcaatct caccgtgcag gtccgccgcg gcgaagtcgc cgacagcgtc
     4261 gacgtcggca caattattcc gctcggcaag ggcatctcgg gccaagtcgc cgtcacaggt
     4321 gagccggtat gcggacatgc cggtgaagga ccagggcttc gacgcgtgcc cagcgagccg
     4381 gacgcggcgt cagtgatcgc cgtcccgctg aagcagtcgg gccggatcgt gggcgtgttg
     4441 aacctttacg acaaggaggg cgcgcgcgag ttcacgccgc acgacctcga cacgattctc
     4501 gcgtttagca cgcaggcggg cgtcgcgatc gacaacgtgc tgttgcatca ggaggcgcag
     4561 cgtctgagcc tcaccgatcc gctgaccggc ctgtggaatt accgctacct cacgatcggc
     4621 ctgggccacg aaatcgagcg cgcgacccga ttcagtcggc cgctcgcggc actgatgctc
     4681 gacctcgacc ggttcaagtc gatcaacgac aagtacggac atcaggtcgg cgacgcggtg
     4741 ctgatcgagt tggccggccg gatgcgcgcc gaggtccgcg aggtcgacac cgtcgcgcgc
     4801 tacggcggtg aggagttcgt gatcgtgctg ccggagacgg acgccattgg tgcggcgcgc
     4861 cttgcgactc ggctcggaac cgtgatcagg tcgaccccgt tctgcctcga cctcggctcg
     4921 aacgggcacg cggtcgatcc gcacaaggga ctgcaggtga ccgcgtcgat cggcgctgcc
     4981 gttttccccg agcacggcat gacgccgtcg cggttgctac gcagcgccga cgatgctttg
     5041 tacgccgcga aggacgcggg ccgcgacggc tggcgctttg gacttgccac cgaggacgac
     5101 ggcacgaacg agcaacgccg agttctcgtc gtcccggaca ccgatattgc gctacctaag
     5161 agcgcagatc gcccccgttt ccccgacgac tgacccggtt cgcccgatgg ctgacccggt
     5221 tcgcccgatg actagcgcaa aacgttaggg ttcggtcatg gcggatcaga aggtcaccaa
     5281 ggccgtcatc ccggccgccg gtctcggcac ccgattcctg cctgccacca aggcgacgcc
     5341 gaaagagatg ctcccggtcg tcgacaagcc ggcgatccag tacgtcgtcg aggaggccgt
     5401 cgccgcc
//
LOCUS       DATCBJ010000526         3255 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_12750, whole genome shotgun sequence.
ACCESSION   DATCBJ010000526 DATCBJ010000000
VERSION     DATCBJ010000526.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 3255)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3255)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3255
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_12750"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..107
                     /locus_tag="VII50_10645"
     CDS             <1..107
                     /locus_tag="VII50_10645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016473395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HEY5051350.1"
                     /translation="LGRAMLKKLKVYSGPNHPHAAQQPVPFEITQVAQ"
     gene            301..705
                     /gene="rpsI"
                     /locus_tag="VII50_10650"
     CDS             301..705
                     /gene="rpsI"
                     /locus_tag="VII50_10650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009376128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HEY5051351.1"
                     /translation="MRRRSASGSGNATGRRKEAVARVRLVPGTGQIKVNGRTIESYFP
                     NKVHQQLVKSPLAALDGVEQFDIVARITGGGISGQAGALRLGIARALILIDPENRPTL
                     KKAGYLTRDARVIERKKYGLKKARKAPQYSKR"
     gene            710..2095
                     /gene="glmM"
                     /locus_tag="VII50_10655"
     CDS             710..2095
                     /gene="glmM"
                     /locus_tag="VII50_10655"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HEY5051352.1"
                     /translation="MPDNSVRVMDGELTRFRLFGTDGVRGVANQELTAELALELAVAA
                     AHVLGEAGAFEGHRPLAVVGRDPRASGEFLEAAVVAGLASAGVDVLRLGVLPTPAVAY
                     LTADLKADLGVMLSASHNPMPDNGIKFFARGGHKLADELEDAIEARLLEEWQRPIGAG
                     VGRVHDYPEATDRYVDYVVSTVPNRLDGLKVVVDCANGAASFAAPEALRRAGATVIAV
                     GCEPDGLNINEGCGSTHLDLLSAAVVANGADVGVAHDGDADRCLAVAADGSVVDGDQI
                     LAILSIAMNETGRLAQGTVVATVMSNLGFRLGMEAAGIKVVETAVGDRYVLEAMRADG
                     YTVGGEQSGHVILLDYATTGDGILTALQLLARMTQTKQSLIELAAVMHRLPQVLINVT
                     DVDRTRAETDVELLAAIERASSDLGASGRILLRPSGTEPVVRVMVEAATRAQADAVAG
                     ELARVVRERLG"
     gene            2099..>3255
                     /gene="glmS"
                     /locus_tag="VII50_10660"
     CDS             2099..>3255
                     /gene="glmS"
                     /locus_tag="VII50_10660"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004559947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HEY5051353.1"
                     /translation="MCGIVAYVGPQPARDVVLAGLRRLEYRGYDSAGIAVLADDLVHV
                     RRKAGPLSNLELVLETDPLPEGGTGLGHTRWATHGRPTDENAHPQQDCTGGLALIHNG
                     VIENHADLRARLIKDGHTFRSQTDTEVAAHLLEEAFAACGDLAKSLRQVCRCLDGSFS
                     FVAVHRDAPGLVVGARRNSPLVVGVGDGEMFLASDVAAFIAHTRDAVELGQDQVVAVR
                     ADGFEITTFDGAVVDGRHYHVDWDLSAAEKGGYDYFMLKEIAEQPEAVAATLLGRLTH
                     DGALMLDEMRLDEAMLRDVDKIVVVACGTAYHAGLIAKYAVEHWANLPCEVELASEFR
                     YRDPILDGRTLVIAISQSGETLDTLMAVQHAREMGARVLAICNTVGSTIAR"
BASE COUNT          549 a         1063 c         1108 g          535 t
ORIGIN      
        1 ccctcgggcg cgccatgctg aagaagctca aggtctactc aggcccgaac cacccgcacg
       61 cggcgcagca gccggtgccg ttcgagatca cccaggtcgc ccagtaatcg tggcgacact
      121 gacgcagcta ccggccggta ccgcgcccct tggcgcctcg gtcgcgacca tgagaagtta
      181 aggagctccg tggcagacac cctgctgacc accgacgacg acgctgagat catcggcgcc
      241 gctgacgacg gactcgagtc ggccgagacg ccgctcacct attcgagcga gaccacgacg
      301 gtgcgtcgcc gctcagcgag cggctccggc aacgccactg ggcgtcgcaa ggaggcggtc
      361 gcccgcgtgc ggctggttcc cggcaccggt cagatcaagg tcaacggccg caccatcgag
      421 agctacttcc cgaacaaggt ccaccagcaa ctggtgaaga gcccgctagc ggcgctcgat
      481 ggcgtcgagc agttcgacat cgtggcccgc atcaccggcg gtggcatcag cggccaggcg
      541 ggtgcgctgc gtctcgggat tgcccgcgcg ctcatcctga tcgaccccga gaaccgcccg
      601 accctcaaga aggccggcta cctcacccgt gacgcccgtg tcatcgagcg taagaagtat
      661 ggtctgaaga aggcccgcaa ggctccgcag tactccaagc ggtaaccgcg tgcccgacaa
      721 ctcggtgcgc gtcatggacg gcgagctgac tcgcttccgg ctgttcggca cggacggcgt
      781 tcgcggtgtc gcgaaccagg agctgacggc cgaacttgcc cttgagctcg ctgtcgcagc
      841 ggcccacgtg ctcggcgagg ccggtgcgtt cgaaggccat cggccgctcg cggtcgtcgg
      901 ccgcgacccg cgtgcatcgg gggagttcct cgaagcggcc gtcgtcgcgg gcttggctag
      961 cgctggcgtc gacgtcttgc gactcggtgt tctcccgaca cccgcggtcg cgtacttgac
     1021 cgctgatctg aaggccgacc tcggcgtgat gctctcggcc agccacaacc cgatgcctga
     1081 caacggaatc aagtttttcg cacgcggcgg ccacaagctc gccgacgaac tcgaggacgc
     1141 gatcgaggca cggctgctcg aggaatggca gcgcccgatc ggcgccggcg tcggtcgcgt
     1201 gcacgactat cccgaggcga ccgaccgcta cgtcgactac gtcgtgtcga cggtgccgaa
     1261 tcggctcgac ggtctcaaag tggtggtcga ttgcgcgaac ggtgccgctt cgttcgccgc
     1321 gccggaggcg ctgcgccgcg cgggcgcaac ggtcattgca gtcggctgcg agcccgacgg
     1381 cctcaacatc aacgaaggct gcggctcgac ccacctcgat ctgctgtcgg ccgcggtggt
     1441 ggcgaacggc gccgatgtcg gcgtcgcaca cgatggcgat gcagaccgat gccttgcggt
     1501 cgcagcagat gggtccgtcg tcgacggcga ccagattctg gccattctct cgatcgcgat
     1561 gaacgagaca gggcggcttg cgcagggcac agtcgtcgcg accgtgatgt cgaatctcgg
     1621 atttcggctc ggcatggagg ccgcgggcat caaggtcgta gagacggcgg tcggcgaccg
     1681 ttacgtgctc gaagcgatgc gcgccgatgg gtacacggtc ggtggcgagc agagcgggca
     1741 cgtgatcctg ctcgactacg cgaccaccgg cgacggcatc ctcacggcgc tgcaattgct
     1801 cgcgcgcatg acgcagacca aacagtcgct catcgaactc gccgctgtca tgcaccggtt
     1861 gcctcaggtc ctgatcaacg tcaccgatgt cgacagaacg cgagcggaga ccgacgtcga
     1921 gttgctcgcc gccatcgagc gggcgagtag cgatctcggt gcatcgggcc ggatcctgct
     1981 tcgccctagc ggaaccgagc cggtggtccg cgtgatggtc gaggcggcca ctcgagccca
     2041 ggccgacgcc gtcgccggag agctagcccg ggttgtgcgc gagcggttgg gttagaccgt
     2101 gtgcggcatc gtggcctatg tcggacctca gccggctcgt gacgtcgtcc tcgccggtct
     2161 gcggcgattg gagtaccgcg gctacgactc agccggcatc gcggtgctcg cagacgattt
     2221 ggtgcacgtg cgtcgcaagg ccggcccgtt gtcgaacctc gaactcgtgc ttgagacgga
     2281 tccgttgccc gaggggggca ccgggcttgg gcacacgcgt tgggccacac acggccggcc
     2341 gaccgacgag aatgcgcacc cgcagcagga ttgcaccggc gggctcgctc tgattcataa
     2401 cggcgttatc gagaaccacg ccgatctacg ggcgcggctc atcaaagacg gtcacacgtt
     2461 tcgatcgcaa accgacaccg aggtcgcggc gcacctgctt gaagaagcgt tcgctgcgtg
     2521 cggagacctt gccaagtcgt tgcggcaggt gtgtcgatgc ctcgatggct cgtttagctt
     2581 tgtcgcagtg catcgcgacg cgccggggct ggtcgtcggg gcgcggcgca attcaccgct
     2641 cgtggtcggc gtcggcgacg gggagatgtt tcttgccagc gacgtagccg cgttcatcgc
     2701 acacacgcgc gacgcggtcg aactcggaca ggaccaggtg gtagcagtgc gcgccgacgg
     2761 gttcgagatc accacgttcg atggtgcggt cgtcgacggc cgtcactacc acgtcgattg
     2821 ggatctgtcg gccgccgaga agggcggcta cgactacttc atgttgaaag agatcgccga
     2881 acagccagag gcggtggccg ccacgttgct cggccgactg acccacgatg gcgcgttgat
     2941 gctcgacgag atgcgtctcg atgaggccat gctccgcgac gtcgacaaga tcgtggtggt
     3001 ggcttgcggc acggcctacc atgccggcct catcgccaag tacgccgtcg agcactgggc
     3061 gaacctgccg tgcgaggtcg agttggcgag tgaattccgc taccgcgacc cgatcctcga
     3121 cggtcgcacg ctggtcatcg cgatatcgca gtcgggcgaa acactcgaca cgttgatggc
     3181 ggttcagcat gcgcgcgaga tgggcgcgcg ggtgctggcg atctgcaaca cggttgggtc
     3241 gacgatcgcc cgtga
//
LOCUS       DATCBJ010000527         2011 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_386907, whole genome shotgun sequence.
ACCESSION   DATCBJ010000527 DATCBJ010000000
VERSION     DATCBJ010000527.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2011)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2011)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2011
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_386907"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..754
                     /locus_tag="VII50_10665"
     CDS             <1..754
                     /locus_tag="VII50_10665"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEY5051354.1"
                     /translation="VLRTAVDSGINHIDTAQYYGPGVVNELIREALYPYPSDLAIVSK
                     VAARRDDTGAVLPYDEPDQLRAGIEDNLTTLGAERLAAVNLRLMDGSTPGARFDAQLG
                     ALVAARDEGLIDGIGLSNISLDHLLRAVAQTEIVCVQNLFNLVDQRSFGVLDECRSRK
                     IAFVPFFPLGWPRGAQNGVLSSPIVAELATRLGATPSQLALAWLLDLAPNILLIPGTR
                     TREHLAENIHAAGVQLDDAARAELAERFPTID"
     gene            complement(804..>2011)
                     /locus_tag="VII50_10670"
     CDS             complement(804..>2011)
                     /locus_tag="VII50_10670"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEY5051355.1"
                     /translation="NNAAGTQISQSSEPLNQADCGPAIPTLTITAVNALHGKPFTVPS
                     RNGSERWRVVVSVTPSGTGSIAVATPLTGVDSTLNQLVLFDVIIGAGVVIVLAGLSYI
                     AVRHSLRPLVDVEHTAAAIAAGDLTRRVPQSDPRTEVGRLAGALNGMLSQIESAFRAR
                     EASEQEARTSEHRMRRFITDASHELRTPLTSIRGFAELYRMGAVPEESDVDRVMTRIE
                     SESTRMGLLVDDLLLLARLDQQRPLEREIVDLETIASDAVHDAQAVAPGRDVELQLST
                     HEPVLVVGDDARLRQVVGNLVTNALTHTPESAAVTVGLSIDDSAAERFAVLEVADSGP
                     GLSPDDARRIFERFYRVEESRTRANGGSGLGLSIVAALTAAHGGTAEVESKLGIGSRF
                     RIRLPLAEM"
BASE COUNT          341 a          647 c          670 g          353 t
ORIGIN      
        1 cgtactgcgc acggcggttg acagcggtat caaccacatc gatacggcgc agtactacgg
       61 gcctggtgtt gtcaacgagc tgatccgaga agcgctgtat ccgtatccaa gtgacctggc
      121 gatcgtgagc aaggtggccg cccgccgcga tgacaccggt gctgtcctgc cgtatgacga
      181 gcccgatcag cttcgcgccg gcatcgagga caacctgacg acgctcggcg ccgaacgatt
      241 ggcagcggtg aaccttcggc tgatggacgg gtcgacgcct ggtgcgcgat tcgacgccca
      301 gcttggtgcg cttgtggctg cccgcgacga aggcctgatc gacggcatcg gcctgagcaa
      361 catctcgctc gaccacctgt tgagggcggt cgcccagacc gagatcgtct gcgtgcagaa
      421 cctgttcaac ctcgtcgatc aacgctcatt cggtgtactc gacgagtgca ggtcgcgaaa
      481 aatcgcgttc gtgcccttct tcccgctcgg ttggccccgc ggcgcgcaga acggagtctt
      541 gagcagccct atcgtcgcgg agcttgcaac acggctggga gcgacgccat cacagcttgc
      601 actcgcgtgg ctgcttgacc ttgcgccgaa catcctgctc attcccggga cacgtacgcg
      661 agagcatctc gcggagaaca tccatgcggc aggcgtgcag ctcgacgacg ccgcacgcgc
      721 ggaactagcg gagcgattcc cgaccattga ttgaccgcgt gtgacgcgcc ggtattcgcg
      781 acgcacgcgg cggtgcgacg gtgtcacatt tcggcgagtg gcagccggat gcggaagcgc
      841 gagccgatgc cgagtttcga ctcgacctcg gcagtaccgc cgtgagccgc ggtcaatgcg
      901 gcgacgatcg agagtccgag gccgctgcca ccattggcac gggtgcggga ttcctcgacg
      961 cggtagaagc gctcgaagat gcggcgcgcg tcgtccgggc tgagccctgg tccggagtcg
     1021 gcgacctcga gcaccgcgaa ccgctcggcc gcgctgtcgt cgatgcttaa gccgaccgtg
     1081 accgccgcgg attccggcgt gtgcgtcagc gcgttggtga cgagattgcc gaccacttga
     1141 cgcagccgcg cgtcatcgcc gacgacgagc accggttcgt gcgtcgaaag ctgcagttcg
     1201 acatcgcggc ccggcgccac ggcttgggca tcatgcacgg cgtccgaggc aatcgtctcg
     1261 aggtcgacga tctcgcgctc aagcggccgt tgctggtcga ggcgagcgag cagcagcagg
     1321 tcgtcgacga gcaagcccat gcgagtcgac tcgctctcaa tccgcgtcat gactcggtcg
     1381 acgtccgatt cctcggggac ggcgcccatg cggtacagct ccgcgaatcc gcggatggat
     1441 gtcaacggcg tccgcaactc gtggctcgcg tcggtgatga agcgacgcat gcgatgctcc
     1501 gacgtccgcg cttcttgttc cgatgcctca cgcgcgcgaa atgccgattc gatttggctc
     1561 agcatgccgt tcaatgcgcc cgcgaggcgg ccgacttctg tgcgtggatc gctttgcgga
     1621 acgcgtcgag tcagatcccc agctgcgatg gcggcggccg tgtgttcgac gtcgaccagt
     1681 ggccgcaagc tgtgacggac ggcgatgtag ctcagcccgg ccaggacgat caccacgccg
     1741 gcgccgataa tgacgtcgaa gagcaccagc tgattcaagg tgctgtcgac gccagtgagc
     1801 ggtgtcgcga cggcaatgct gccggtgccg ctcggcgtga ccgacaccac gacccgccaa
     1861 cgctcactgc cgttgcgtga cggaaccgtg aacggcttgc cgtgcagcgc attcactgcg
     1921 gtgatcgtga gagtcgggat ggccggcccg cagtcggcct ggttcaaggg ctcgctactc
     1981 tgcgaaatct gggtgccggc ggcgttgttc a
//
LOCUS       DATCBJ010000528         2015 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_69057, whole genome shotgun sequence.
ACCESSION   DATCBJ010000528 DATCBJ010000000
VERSION     DATCBJ010000528.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2015)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2015)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2015
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_69057"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1843
                     /locus_tag="VII50_10675"
     CDS             <1..1843
                     /locus_tag="VII50_10675"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY5051356.1"
                     /translation="RMSRDMSSILPVAGGRAAWRSLRSGLAERRGLIAAAIALQMAAS
                     AAGLVGPQLLERIINSSGTLTDATIDSTALLFTVALVVQTIFTAGARACGALTGEYVL
                     AQLRERFIATVLALPIGIVERAGTGDLMTRASTDVDDMSQAVRRGLPELLVAGVTGVL
                     AIVALVVTAPILGLVVVPVIPPLVFATRWYLRRARPAYQAQAAAEAVVNADLQETVNA
                     ARTIESLRLGPRRITQTDDDIRDWIAWERRTLRMRTIFFGTCEAAYIVPLVLCLLFGG
                     LLVIHGQLTIGAVAAAALYTQQLVSPVDTLLAWQDEVQLASASLSRVVGVSEVPPAEM
                     TDRIPADQQLVADQVRFAYNDGADVLHGIDLSPDVGSRLAVVGPSGAGKSTLALLLAG
                     VHAPRTGAVEVGGVEPHLLPPDRLRREVALVTQEHHLFACSLRDNLRLARPGAGDEQL
                     LAVLETVDATQAVRALPDGLDTVIGSGGTSISPGFAQQIAIARLLLADPHTLVLDEAT
                     SLLDSRAARHLERSVGVLLEGRTVIAISHRLHAAHDAEIVVVVEDGRISEYGTHADLL
                     KAGGAYARLWDTWRQDAADDDQVNGGPSPENVDTTGRTDTLPSEQKT"
BASE COUNT          300 a          667 c          692 g          356 t
ORIGIN      
        1 acgaatgagc cgcgacatgt cgtccatcct cccggtcgcc ggcggtcgcg cggcgtggcg
       61 tagcctcagg tcgggccttg cggagcgtcg cggattgatc gccgcggcca tcgcgttgca
      121 gatggcggcc agcgccgccg ggctggtcgg accgcagctg ctcgagcgaa tcatcaacag
      181 ctcggggaca ctcaccgacg ccaccatcga cagcaccgcg ctgctgttca ccgtcgccct
      241 ggtggtgcag acgatcttca ccgcgggtgc ccgtgcgtgc ggtgcgctca ccggcgagta
      301 cgtcctcgcg cagctgcgtg agcggttcat cgcgaccgtg ctcgcgctac cgatcggcat
      361 cgttgagcgc gctggtactg gtgacctgat gacccgtgct tccacggacg tcgatgacat
      421 gtcgcaggcg gtgcggcgcg gccttcccga gctgctcgtg gccggcgtca ccggcgtgct
      481 cgcgatcgtg gcgcttgtgg tcaccgcgcc gatcctcggc ctcgtcgtgg tgccggtcat
      541 cccgccactc gtgttcgcca ctcgctggta cctgcgccgc gcgcggccgg cgtatcaggc
      601 gcaggccgct gcggaagccg tggtgaatgc tgacctccag gagaccgtca acgctgcacg
      661 caccatcgag tcgctgcggc tcggtccacg tcgcatcacg cagacggacg acgacattcg
      721 cgattggatc gcgtgggaac ggcgcacgct gcgcatgcgc accatcttct tcggcacgtg
      781 cgaggcggcc tacatcgtgc cgctggtgct gtgcctgctg ttcggtggtt tgctggtgat
      841 ccatggacag ctcacgatcg gagccgtcgc agcggcggcg ctgtacacgc agcagctggt
      901 gtctcccgtc gacacgttgc tcgcgtggca ggacgaggtg cagcttgcgt ctgcgtcact
      961 ttcacgcgtt gttggcgtgt cggaggttcc acctgcggag atgaccgaca ggatcccggc
     1021 ggaccagcag ctcgtcgccg atcaggtgcg tttcgcatac aacgacggtg ctgatgtcct
     1081 gcatggcatc gatctgtcgc cggacgtcgg ctcgcgtctc gcggtggtcg gtccgagtgg
     1141 agcggggaag agcacgctgg ccttgctgct cgccggtgtc catgcaccac gcaccggtgc
     1201 cgtggaggtt ggtggcgtgg aaccgcacct cctgccgccg gaccgcctcc gccgcgaggt
     1261 cgcactggtc acccaggagc atcacctgtt cgcctgctcg ttgcgtgaca acctccgcct
     1321 cgcacgcccc ggcgccggcg atgagcagtt gctggccgtg ttggagactg tcgatgcgac
     1381 gcaggccgtt cgggcgcttc ctgatgggct cgacacggtg atcggatccg ggggaacatc
     1441 gatcagcccg ggattcgcac agcagattgc gattgcgcgg ctgctgctcg ccgacccgca
     1501 cacgctcgtg ctcgatgagg cgacctcgct gctcgactcg cgtgccgcgc gccacctcga
     1561 gcgatcggtt ggtgtgctcc tcgaaggccg caccgtcatc gccatctcgc accggctgca
     1621 cgcggcgcac gacgccgaaa tcgttgtggt ggttgaagac ggtcgtatca gcgagtacgg
     1681 aacacacgcg gacctgctca aagcaggcgg cgcgtatgcc cggttgtggg atacgtggcg
     1741 gcaggatgcg gccgatgacg atcaggtgaa cggcggtccg agtccggaga acgtcgacac
     1801 gacgggaagg accgacaccc tgccgtcgga acagaagact tgaccgtcct ggaaaccggc
     1861 aacccgcaga ttggcaagca gtggcgtcga cgcccctggg gtcaggacgc ggacacttgc
     1921 cgcggtcaca cacggactga tgacatcgtc gccggcaatg ctgatgtatg ccgtgggggt
     1981 ggaatccgac gcgcacccgc ctgacgcggc gcatg
//
LOCUS       DATCBJ010000529         2645 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_331314, whole genome shotgun sequence.
ACCESSION   DATCBJ010000529 DATCBJ010000000
VERSION     DATCBJ010000529.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2645)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2645)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2645
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_331314"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..347
                     /locus_tag="VII50_10680"
     CDS             <1..347
                     /locus_tag="VII50_10680"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEY5051357.1"
                     /translation="LDQARADGQARDEGRRVRKDGSRFWANAVITAVYEKGGRLEGFA
                     KGTRDETERQRVEEQARLREIFAECDRIANELLATVVRQLVSRWKVRRRRRTIRTYAS
                     VFTTPSILSTVR"
     gene            complement(452..1045)
                     /locus_tag="VII50_10685"
     CDS             complement(452..1045)
                     /locus_tag="VII50_10685"
                     /inference="COORDINATES: protein motif:HMM:NF013935.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HEY5051358.1"
                     /translation="MVAAVRSPAGRAGRMWLRHRIAVAERGASELERKVQLVSTEVRR
                     RKAQVDQSRAQWRAACEEAERWMLRAGFVGGIDSVRQAQTTPVEVAITWTTTMGVRHL
                     DSATIDPSAAPDVGMSAAVLQARQAFATALRASADVATAQSALATLEVALLTTRRRAR
                     VLRRHWLPRLQSQLHNLEITLEQAEQEDQARLRRVSA"
     gene            complement(1054..1938)
                     /locus_tag="VII50_10690"
     CDS             complement(1054..1938)
                     /locus_tag="VII50_10690"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016208 - AMP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEY5051359.1"
                     /translation="MSAEVDHSPIVVGVDGSVGSRAAVGWAIDEATRRGAPLELVHSW
                     RPMYRATHDAAGDSEAEMVARDHGAWLLAAAAGSVSSSAPQLAVTTRLVKGRPSAGLL
                     DAAANAQLLVVGARGVGGYAGLSLGSVSLHVVAHAPSSVVVVRSAREVGPVIVGIDGS
                     AESKVVLRAALDEAILRHSPLVVMSALFVHSRAEGVLDRERALAAAQANASYSLEQLL
                     GAVHPTEYDAIEISQSLPIGYPAEVLANASTASRLLIVGSHGGGGFSGMKLGSIAHAV
                     VHNAHCTVLVVRDRPSTH"
     gene            complement(2102..>2645)
                     /locus_tag="VII50_10695"
     CDS             complement(2102..>2645)
                     /locus_tag="VII50_10695"
                     /inference="COORDINATES: protein motif:HMM:NF012236.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5051360.1"
                     /translation="TPRVALTIAEDLAFEHGRNVLVVMADMTSYAEALREVSAARGEI
                     PGRRAYPGYLYSDLASLSRLMRKGAGPGRTRDDHLDLAAQLLASLSRSQQVRELAELV
                     GVEALSDVDLAFVEFAAKFERTLLAQRPDEKRSLAQTLDLAWSVALALPDRELTMLPP
                     AVLAAHRDSAMLTAAPNAPQ"
BASE COUNT          476 a          872 c          859 g          438 t
ORIGIN      
        1 aacttgacca ggcccgagcg gacgggcaag cgcgtgacga gggcaggcgg gtgcgcaagg
       61 atggcagtcg attttgggcc aacgctgtca tcactgccgt ctacgagaag gggggtcgtc
      121 tcgaaggctt cgctaagggc acgcgcgacg agaccgagcg gcagcgcgtc gaagaacaag
      181 ctcgcctacg tgagattttc gccgagtgcg accgcatcgc gaacgaactt ctggcaactg
      241 tcgtgcgcca gctggtctcg cgatggaaag tgcggcgcag gcgacgaacg atccgaacgt
      301 atgccagcgt attcacgacg ccgtcgatat tgtcgaccgt gcgttgaaag ccagtcgcgt
      361 cgctgtgctt cggtatgact gccggaggta gttgacgacg aggtcggcca gtactcgctg
      421 ccccaatgcg gtcggccgcc tcgccgccct gctatgcact caccctccgc agccgagctt
      481 gatcctcctg ctccgcttgc tcgagagtga tttccaagtt gtgtagctgt gactgcagtc
      541 gcggcaacca gtggcgccgc agcactcgcg cgcggcgacg ggtcgtgagg agggccacct
      601 cgagagtggc gagcgcagat tgcgctgtcg cgacgtcggc cgatgcgcgt agcgccgttg
      661 cgaatgcttg cctggcctgc aggactgccg cgctcatccc gacgtccggg gcggctgacg
      721 ggtcgatcgt tgcgctgtcg aggtgacgca ctcccatcgt cgtcgtccac gtaatcgcga
      781 cctcgaccgg cgtcgtctgc gcctgtcgca ctgagtcgat gccgcccacg aatccggcgc
      841 gcagcatcca gcgctcggct tcctcgcagg cggctcgcca ttgtgcccgg ctctggtcga
      901 cctgtgcttt ccggcgacgc acctcggtgc ttaccagctg aaccttgcgt tccagttcgc
      961 tcgcaccgcg ttcggcaacc gcgattcgat gacgcaacca catgcggccg gcacgaccag
     1021 cgggactccg aaccgcggcg accatcgccg acctcagtgt gtgcttggtc ggtcgcgtac
     1081 gaccaacacg gtgcagtgcg cgttgtgcac gaccgcgtgc gcaatcgacc ccagcttcat
     1141 ccccgagaac ccaccaccac cgtgagatcc gacaatcagg agtctcgatg ccgtggaggc
     1201 gttcgcgagc acctcagccg ggtagccgat gggtagcgat tgcgagatct cgatcgcgtc
     1261 gtattcagtg gggtgtactg cgcccagcag ctgctctaac gagtagctcg cgtttgcttg
     1321 ggcagccgcg agcgcgcgct cgcggtcgag aacaccttcg gctcgcgagt ggacgaagag
     1381 cgcgctcatg acgaccaagg ggctatggcg caggatggcc tcatccaacg cggcgcgcaa
     1441 gaccactttc gactcggccg atccgtcgat tcccacgatc accggaccga cctcgcgtgc
     1501 acttcgcacc acgacaactg acgacggcgc atgagcgacg acgtgcaaag aaaccgagcc
     1561 tagcgaaagt ccggcgtagc cgccgacgcc gcgtgcgccg acgaccaaga gttgggcgtt
     1621 agcggccgca tcgagcaggc ccgcgctcgg ccgccccttt actaggcggg tagtaacggc
     1681 gagttgggga gccgatgacg agaccgagcc agccgcggcg gcaagcagcc aagccccgtg
     1741 atcccgggcg accatttccg cctcggagtc acccgcggca tcgtgggtgg cgcggtacat
     1801 cggacgccag gaatgcacca gctcgagtgg agctccacga cgggtagcct cgtcgattgc
     1861 ccaaccgacc gcagcgcggc tgcctacgga tccgtcaact ccaacgacga tgggcgagtg
     1921 gtccacttcg gcgctcatca ctacctccgt gtatcgacgg ttctcgcgcg gataacgagt
     1981 cctatgtgca gacaacgccc acccgggaag acagggatat ggcccaaggt cccgtctgat
     2041 acggccgggg gtcgccattg tcggcgcgcc cattgcgacg aacgtgtaga caacgcatcc
     2101 gctattgagg cgcgttcggc gcggcggtca gcattgcgga atcacgatgc gcagccagca
     2161 ccgccggcgg cagcatcgtc agctcacggt cgggaagcgc cagcgcaacc gaccatgcca
     2221 agtcgagcgt ttgggcgagg cttcgcttct catcaggacg ttgcgcgagc aaggtgcgct
     2281 cgaacttggc ggcaaattcg acgaaagcga gatcgacgtc actcaaagcc tcgaccccga
     2341 caagctccgc tagctcgcgc acctgttgag accgcgaaag cgacgccagc agttgtgctg
     2401 cgaggtcgag gtgatcgtcc cgggtgcgcc cggggccggc gcccttgcgc atcagtcgcg
     2461 acaacgacgc gaggtcgctg tacaggtatc ccggatatgc gcggcgcccg gggatctctc
     2521 cccgcgcagc cgagacctcg cgaagtgctt cggcgtagct cgtcatgtct gccatcacga
     2581 cgagcacatt ccggccgtgc tcgaaggcga gatcctccgc aatggtgaga gcgacccgcg
     2641 gggtc
//
LOCUS       DATCBJ010000530         2095 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_426394, whole genome shotgun sequence.
ACCESSION   DATCBJ010000530 DATCBJ010000000
VERSION     DATCBJ010000530.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2095)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2095)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2095
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_426394"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(386..616)
                     /locus_tag="VII50_10700"
     CDS             complement(386..616)
                     /locus_tag="VII50_10700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5051361.1"
                     /translation="MSVLVWVTVAVTVLLGLVTVLPGVVTVLPGVVTVLPGLVVAVPG
                     VVVVAPGTVTVVTAPATVLVLNLVCVLELPSR"
     gene            complement(710..874)
                     /locus_tag="VII50_10705"
     CDS             complement(710..874)
                     /locus_tag="VII50_10705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5051362.1"
                     /translation="MPITVTSVYVPASTTVCPLAAAREFTLIVLASLELLLMTNVVAL
                     HPAHDHFSSD"
     gene            1414..1791
                     /locus_tag="VII50_10710"
     CDS             1414..1791
                     /locus_tag="VII50_10710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5051363.1"
                     /translation="MAGSKKPDHDAVKPSEQRGGTTPDETEAHEKGMWAATASEGIVP
                     AELGGSDAPREMLGEDPELGSAVLGVTTGSDKPATEDGIDPAGGDDADATSDGGPDLP
                     PGVEPDLKDAAAVASQPDPPTGR"
BASE COUNT          424 a          688 c          695 g          288 t
ORIGIN      
        1 tcggccgccg cagcttgtag aaccgaaact caagggatca tgccggggcc gcgctgcccg
       61 gcgtgatcga cttgaccgcc gctctgcggt cggcggcgct tcccgtggct tgaaccgctg
      121 tccggcttca acaaggagga ccatggtcgc ttgctatgcc gctggccttt accgtattgg
      181 cctacggagg agagccaatg atgagtacgc ctgagccgcc tgagccgccc gagcggatgg
      241 gcacgcctga gccgcccggg gttagtacgc ctgagccgcg tgagccgatg cgtccgcgcg
      301 atcgtcaagc cgcgatctgg tggcagcgag aaatggcaat tgtcgcagta gggatttttg
      361 ccctgctcgt tggtggtgct gttggctacc tgctgggcaa ttccaatacg cacaccaagt
      421 ttaggaccag aaccgtggct ggcgctgtca cgacggtcac tgtccccgga gcgacgacga
      481 caacgcccgg cacggcaacg acaagaccgg gcaagaccgt gaccactccc ggcaggacgg
      541 tgacgacacc cggcaagacg gtaactaggc ccaacaagac ggtgacggcg accgtgaccc
      601 agactaggac gctcaccacc tcgtcgacct cgaccgctac tccgccggcg gggccgcaaa
      661 gcttcaacgg ggcggacacg cagaatctgg gcactatccg cgtggcgagt cagtcgctgc
      721 tgaagtggtc gtgcgcaggg tgtagcgcca cgacgttcgt catcagcaat aactctaacg
      781 atgccagcac gatcagcgtg aattcacgtg cggcggcgag cggacagacc gtggtcgacg
      841 cgggaacgta taccgatgta acagtgatgg gcaccggtcc ctggtcgttt acgattacgc
      901 ccggaggata atgcccggtt ccggggccga tgtcgggtcg cgaaggttcg cgccaggcgg
      961 catgctctaa gccagacccg ccgggcgacg ccgtcgctag gcgaccacgc cgaccacgag
     1021 cccgcttgaa atggcaacta aacgccacac agatcccttt caggcgctcc gcgcggcggg
     1081 cctgcgcaag aaggtggcgc acgccgtcac agacgcgact cgcaagacca ggcgcggcaa
     1141 gcaatccaaa cttctcgtca agacaatcga gaacttgacc acagcagtcg cagaaatcga
     1201 gcgacgaacg gctggcaaca cgcgcaccga cgcggcgaga aggcagcccc gactcgcaag
     1261 cgcaaaacag cggcgcgcag cacagtcgca cacacggctg ccaaaaccgg cacagacacg
     1321 gaagcctgac gagacggcgg gcgaccagtc aaccgctcgc gcaagcaggt tagttgacga
     1381 ttattacatc ggagtagttg ctaggaggaa ttcatggccg gcagcaaaaa gcctgatcac
     1441 gacgcggtga agccgagcga gcagcgaggc ggaacaacgc cggacgagac cgaggcccac
     1501 gagaagggca tgtgggcggc cacggcgagc gaaggaatcg ttcccgctga gctgggaggc
     1561 tccgacgcac cacgcgaaat gctcggggag gacccggagc tcggcagcgc ggtgcttggg
     1621 gtcacgaccg gctcggacaa gccggcgacc gaggacggca tcgaccctgc cgggggcgac
     1681 gacgccgacg ccacgtccga cggcggaccc gacctgccac cgggagttga gcccgacctg
     1741 aaggacgccg cggccgtggc cagtcagcca gacccaccga cgggccgtta ggccggtccg
     1801 gggcgaccgc atcgccgccg ccgtagcgcg aaaagtttac gagggcgcgc ggctgcacgc
     1861 gcacgcttag ctcgtcgtct ctatgaagga gcatcggcac ctcagctgac cgccgtcgag
     1921 tctgtcctcc caagcacccg cgccgcggat gccgctctgg cctcgcggcc acggcgtccc
     1981 cgcgcaggtc cagtgggacg cattcaggcg cttccgcggc agcgctgccc ggaggtccaa
     2041 ggcgcaccct ggagggactg tggtttgcgg cgacgatcgc cgcctctgct cctgc
//
LOCUS       DATCBJ010000531         2641 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_44159, whole genome shotgun sequence.
ACCESSION   DATCBJ010000531 DATCBJ010000000
VERSION     DATCBJ010000531.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2641)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2641)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2641
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_44159"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1259
                     /locus_tag="VII50_10715"
     CDS             <1..1259
                     /locus_tag="VII50_10715"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020664180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HEY5051364.1"
                     /translation="DHWYHDLVEAESDTCEPEHMESEDLLFTLYTSGTTAKPKGIKHT
                     TAGYHLYTMFTHDLVFDIQPDTIYWCAADVGWVTGHSYIVYGPLGNGVTSVMYEGTPD
                     YPAKDRWWSIIEKYGVTVLYCAPTAIRTFMKWGAEFPQQHDLSSLRLLGTVGEPINPE
                     AWMWYREHIGGDRTPVVDTWWQTETGGIMIAPLPGVTVLKPGSATKPLPGIGAAVVDE
                     SGTPVALGGGGYVTLTKPWPGMLRGIWGDDERYKETYWNRFGTQYFAGDGARIDEEGY
                     FWFMGRVDDVMNVAGHRISTAEVESALVDHPAVAEAAVVAKSDPETGQAISAFVTLRA
                     GYEESTELIAELRDHVAKLIGPIAKPKWIAFTPDLPKTRSGKIMRRLLRDIAENRKLG
                     DTTTLADSSIVADLQRRATEEEAKEQ"
     gene            1343..1972
                     /locus_tag="VII50_10720"
     CDS             1343..1972
                     /locus_tag="VII50_10720"
                     /inference="COORDINATES: protein motif:HMM:NF013358.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate uptake transporter"
                     /protein_id="HEY5051365.1"
                     /translation="MATAVTTGPPYVAESVAPVANPAPLGLVGFGLTTLLLSVINAGF
                     ISAAPGTPTAGSLSVVLATAIAFGGVGQILAGMWEFKVGNTFGATAFTSYGLFWISFV
                     LIETTFGPDLPKDPTPILGMYALGWGIFTAYMFVASLGGASRGVSLVFLLLALTYITL
                     AIAFWGKSLPSAGWNQISGILGLLTAIAAIYCSFAFVTNANFKRTVLPV"
     gene            2195..>2641
                     /locus_tag="VII50_10725"
     CDS             2195..>2641
                     /locus_tag="VII50_10725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5051366.1"
                     /translation="MNTDRRPPTFLIGAARSGTSLLYKGLCLHPEAAYFNNYVRRLPR
                     HPAISVTNRIARMTPEHRRRVWFSGGSNAYVYSGARKLRDRLYPMPVEGEPVYAACGI
                     PDTVSSDTVSMNAARELRRAVHSTMRWGGGTHFINKRIANVRRIPLL"
BASE COUNT          469 a          870 c          843 g          459 t
ORIGIN      
        1 gcgaccactg gtatcacgat ctcgtcgagg ccgagtcgga tacgtgcgag ccggagcaca
       61 tggagtcgga ggacctcctc ttcacgctct acacgtccgg cacgacagcc aagccgaagg
      121 ggatcaagca caccaccgcg gggtaccacc tctacacgat gttcacccac gacctggtgt
      181 tcgacatcca gccggacacg atctattggt gcgcagccga tgtcggctgg gtgaccgggc
      241 actcatacat cgtttatgga ccgctgggca acggcgtgac ctcggtgatg tacgagggga
      301 cgcctgacta cccggccaag gaccgctggt ggtcgatcat cgagaaatac ggggttacgg
      361 tcctctactg cgctccgacg gccatccgta ccttcatgaa gtggggagcc gagttcccgc
      421 agcagcacga cctctcgagc ctgcgcctgc tcggcaccgt gggcgagccg atcaaccccg
      481 aggcgtggat gtggtaccgc gagcacatcg gcggcgatcg cacgccggtg gtcgacacct
      541 ggtggcagac cgagaccggc gggatcatga tcgcgccact ccccggcgtg accgtcctca
      601 agcccgggtc cgcgaccaaa ccgctcccgg gcatcggtgc tgcagtggtg gacgaaagcg
      661 gcacgccagt cgcgctcggt ggcggcggct acgtgacgct gaccaagccg tggccgggaa
      721 tgctgcgggg catctggggc gacgacgagc ggtacaagga gacgtactgg aaccggttcg
      781 gcactcagta cttcgccggt gacggcgctc gcatcgacga agagggctac ttctggttca
      841 tgggccgcgt ggacgacgtc atgaacgtcg ccggccaccg catctcgacc gccgaggtgg
      901 agtccgcgct cgtcgaccac ccggccgtcg ccgaggccgc ggtcgtggcc aagagcgacc
      961 ccgagaccgg acaggcgatc tcggccttcg tcaccctgcg tgcgggctac gaagagtcaa
     1021 ccgagctcat tgccgagctg cgcgatcacg tcgccaagct catcggtccc atcgcgaagc
     1081 ccaagtggat cgcgtttacg ccagatctgc cgaaaactcg cagcggaaag atcatgcgcc
     1141 gcctgctccg cgacatcgcc gagaaccgca aactcggcga caccacgacg ctcgcagatt
     1201 cgagcatcgt cgcggatctc cagcgccgcg ccactgagga ggaagccaaa gaacagtaga
     1261 tccgtcgcag tgggggacag ttcgattacg aacacgcgcc ggagtgcgat ctactccgga
     1321 ctcattggag ggctaccaac ctatggcaac cgcagttacg accggcccgc cctatgtggc
     1381 ggagtcggtg gcaccggtgg caaacccagc gccgctggga ttggtggggt ttggactgac
     1441 aaccctgctg ctctcggtca tcaacgccgg cttcatctcg gctgctccgg ggactccgac
     1501 cgctggttcg ctgtctgttg tcctggcaac ggcaatcgcc ttcggtggag ttgggcagat
     1561 tttggccggc atgtgggagt tcaaagtcgg taacacgttc ggggcaaccg cgttcacgag
     1621 ttacgggttg ttctggatca gcttcgtgct gatcgagacg acgttcggac cggatctccc
     1681 caaggacccg acccctatcc tcggcatgta cgcgcttgga tggggcatct tcacggccta
     1741 tatgttcgtc gcctcgctgg gcggggcgtc gcgcggagtg tcgctcgtgt tcctcctcct
     1801 cgcactcacg tacatcacgc tggcgattgc cttctggggc aagagcctgc cgagcgcggg
     1861 ttggaaccag atctccggca tcctcggcct tctcactgcg atcgcggcga tctattgctc
     1921 gttcgcgttt gtgaccaacg ccaacttcaa gcgaaccgta ctgcccgtct gagggctagc
     1981 ggtaacggtg gcgcgcggcc cttcggggcc gcgccgctgc ttctcgggtg gggagcacca
     2041 gcgttcggcc cgctcttcag gatcacgcgg cggtccgcaa cgcaggccag accgccggcc
     2101 ggtcgtccgc aggcgacggg tcaacgtaac ggaacgccaa cgaacgcgtt tgcgtcaagg
     2161 tgaccaggcg gccctatgcg agaatcgctc atcggtgaac acggatcgac gaccgcccac
     2221 attcctgatc ggcgccgccc gttccggcac aagtcttctc tataaaggac tgtgcctgca
     2281 tccggaggcg gcgtatttca acaactatgt acgtcgcctg ccgcgccacc cggcgatcag
     2341 cgtcaccaac cgcatcgcgc ggatgacgcc cgagcatcgt cgccgcgtct ggttctcggg
     2401 cggctccaat gcctacgtct attcgggcgc gcgcaagctg cgcgatcggc tctacccgat
     2461 gccggtcgag ggcgaaccgg tgtacgcggc ctgcgggatc cccgacaccg tgtcgagcga
     2521 cacggtgagc atgaacgctg ctcgcgagct gcgccgcgcg gtccacagca cgatgcggtg
     2581 gggcggtggg acgcatttca tcaacaagcg catcgccaac gtccgccgca tcccattgct
     2641 t
//
LOCUS       DATCBJ010000532         2648 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_13533, whole genome shotgun sequence.
ACCESSION   DATCBJ010000532 DATCBJ010000000
VERSION     DATCBJ010000532.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2648)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2648)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2648
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_13533"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            249..322
                     /locus_tag="VII50_10730"
     tRNA            249..322
                     /locus_tag="VII50_10730"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:283..285,aa:Ile,seq:cat)
     gene            830..1162
                     /locus_tag="VII50_10735"
     CDS             830..1162
                     /locus_tag="VII50_10735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5051367.1"
                     /translation="MVDVGQRATNGDHASVKVDVLPRQSDRLAATQSAHPDEVPHRVQ
                     VVGRGEVEELPVLRRRPHSHPVTFACAFPFGDPLGSPHDCARRIGVAELDVLAGVAGD
                     HLSAHRLT"
     gene            complement(1902..>2648)
                     /locus_tag="VII50_10740"
     CDS             complement(1902..>2648)
                     /locus_tag="VII50_10740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5051368.1"
                     /translation="SDGAEVMMLARTRDDVDQLNALAKIAAQAAGDSRGPQLVVGDKS
                     FQAGDVIRTKRNSRAITLGETHVRNGDRYTVLATTEAGGLLVDDLTGKGRTMLPPTYV
                     AEHVEHGWATTIDAAQGATTDIAILLVRPGIDREHLYVGLTRGRDENHVYVASPVDED
                     HEHSPAGDTDAQALLQTALGRSGSKDAAHTVIDRANAANRPALPVPSAPAIHTVESEE
                     AAARSRRLATQEVIDQHRSAERRTGRGIGI"
BASE COUNT          371 a          856 c          907 g          514 t
ORIGIN      
        1 tcccggtcgc gcatgcgcga ccgtccgcac cgcgccgcga acgttgacga gctgcggcgg
       61 ccgcgttcgg cgcccacttc atcgggcttg ccgcttattg ccgacgatgg cgttcgtcat
      121 gaggtctgca ccgctgcgac acacgttgtt tacaacgagg tgcacgcaga cgtaaccggc
      181 ggtcgtttgg atcagagctt tggtgcccca gggttgaatg ctcgataggc tcctgacgtc
      241 gcaatacagg ggcggtagct cagccggtca gagcagcgga ctcataatcc gtcggtcctg
      301 ggttcgagcc ccagccgccc cacccttcct gacctgcacc gatgtgtgag accccaccgg
      361 ttgccctgtc cgatttgtcg ctacgtgggc acgatgtggg cacggaactg ttagaggcct
      421 tgattttgcg cggcacgatc gccgctgtgc gctccgcggc gtcgtgcgcg acctcggccg
      481 tcaccgacga gtagatgtcg gatgtgatct tgatcgacga gtgcccgagt tgctcgctga
      541 cggtcttcat cgccactcca gctgacaggc cgagagtcgc ggcgacgtgt cgcagatcgt
      601 gcagtcgcac gggtggcaag ccggatcgtg cgatgaggcg ctcgaaccgt tgactaacac
      661 cgtcagggtt caattgttcg ccggttacag acgcaaacac gaggttcgtc tgacgtagcg
      721 tgcggtgcac ctgtgcggcc cgatgcgcac gcagcacctc gacggttccg gagtcgaggc
      781 tgaccaacct gtcgccgtat ttggacttcg gcgcgcccca caccgggcga tggttgacgt
      841 tggtcaacgt gcgacgaatg gcgatcacgc ctctgtcaag gtcgacgtcc tcccacgaca
      901 atccgaccgc ctcgccgcga cgcagtccgc gcatcccgat gaggtgccac atcgcgtaca
      961 ggtcgtcggc cgcggcgaag tcgaggaact gcccgtactg cgccgccgtc cacacagcca
     1021 cccggtgacg tttgcctgtg cgtttccatt cggcgacccg ctcgggagtc cacacgactg
     1081 cgcgcggcga atcggtgttg ccgaactcga cgtgttggcc ggggttgcgg gcgatcatct
     1141 ctcggcgcat cgcctcacct aacgcgacga gcgcggtcgc gtgcacgcgt cggatcgtcg
     1201 cctccgacgg cttgcgtcgg caaccggcgg ggtccttgcg tgcggcggtg atgcgcagca
     1261 gtgtttgcga tgggcgcgca ggcaacgtcg gaccgatttg ccgcatcgcc cgatagagct
     1321 cctcgaagtc gaacgcgcgc aagtcgatca gccgcacgtg gcccaggccc ggctcgaggt
     1381 agatgtcgat gtggcgtgtg tagttcgacg cagtcgaggg cctcagggcg acgcggcccg
     1441 cgagccactg ccgaaggtag gcgccgaagg tgatcgtgcg gtcgccgagc ggcgccccct
     1501 tatttgtgcg gtcgaccgac tccgctagcg ccgcttcggc ctcggccttc gttgggtagg
     1561 ggccgatcct cagctgcgtg cgcttctggt ccgcggctgg aggtgcgtcg aagcgcgccc
     1621 accacgagcc gtgtccgcga ccgctgagct tcgggcatcg cacgcccagc tgctttcctg
     1681 actcgtcgcg acacccgcac cgcttgaacg tcgagccctt catcggtcgc tccatgtctg
     1741 cagtagccgg acaccgctag ttgcttgtcg caccttttca cgtgggtgcg tcatctggtt
     1801 tctccttcga acggtccggg cggtgcagtc agctttcgcg ccagtgtcga ggtggaggta
     1861 gacccgggca tccgggacgt gcgcatgtcg gcggcccatc ctcatatgcc gatgccgcgg
     1921 ccggttcggc gttcggcgct ccggtgctgg tcgatgactt cttgggtggc gagtcggcga
     1981 ctgcgggccg cggcctcctc cgactcgacc gtgtggatcg ctggcgccga tggcactggc
     2041 agtgcgggtc ggttggcggc gttggcgcgg tcgatcacgg tgtgggcggc gtccttgctc
     2101 ccgctgcgcc cgagggcggt ctggagcagc gcctgtgcgt cggtgtcgcc tgcgggtgag
     2161 tgctcgtgat cctcgtcgac tggcgacgcc acatacacgt ggttttcgtc tcgtccgcgg
     2221 gtgaggccga cgtagaggtg ttcgcggtcg atgccggggc gcacgagcag gatcgcgatg
     2281 tcagtggtgg cgccttgggc ggcgtcgatg gtggtggccc agccgtgttc gacatgctcg
     2341 gccacatatg tcggcggcaa catcgtgcgg ccctttccgg tgaggtcatc gacgagtaga
     2401 ccgcccgcct cggtggtggc gagcactgtg tagcggtcgc cattgcgcac atgcgtctcg
     2461 cccaaggtga tcgcgcggct gttgcgcttc gtgcggatca cgtccccggc ctgaaaactc
     2521 ttgtcgccga cgacgagctg cggaccgcgc gaatcaccgg cggcctgggc cgcaatcttg
     2581 gcgagcgcgt tgagttggtc gacgtcgtcg cgggtgcggg cgagcatcat cacctcggcc
     2641 ccatccga
//
LOCUS       DATCBJ010000533         2108 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_247561, whole genome shotgun sequence.
ACCESSION   DATCBJ010000533 DATCBJ010000000
VERSION     DATCBJ010000533.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2108)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2108)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2108
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_247561"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(164..>2108)
                     /locus_tag="VII50_10745"
     CDS             complement(164..>2108)
                     /locus_tag="VII50_10745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEY5051369.1"
                     /translation="DPLAPPYDIAQALDELGDAVLDGASTSDALRDLLRQGAPGLRGL
                     EDMLRRVRQQRRALRSKGRLDGTLEQARALLDKAVGQERAALFPDPSDDARLRETELD
                     TLPNDPARAIRALADYDWRSDEARATYEELKDLLRQEVLDSQFRGMKQAMHSTQSGSP
                     ESAAAMQAVKDMMADLNEMLAADARGEHTQDDFDQFMAKHGQFFPDQPANLEELVDSL
                     ARRAAAMERMLASMTAEQRDELAALMAQSMEDMGLASEMARLNDALRSRRPDLDWTGR
                     TRMNGNQPLGMSDATTALEELADLEELESTLGQEYPGASLEDVDEEAIRRALGRQAVD
                     DLRQLREVERELERQGYLRRDGGRLELTAKAVRRLGATALRRVFASLSGSTRGDHDVP
                     DAGSAGELTGSSRAWQFGDEQPLDVVRTVSNAILRSGPALSEETGGGVKLQVDDFEVV
                     ETERRTSAAVCLLVDLSWSMTLRGTWGIAKSTALALHSLVSTRFPQDALQVIGFSNYA
                     RVLQPTELAGLDAEMVQGTNLQHALLIAGRFLDRHPEHEPVVLVVTDGEPTAHLLPSG
                     DYTFDWPPSPETLAVTLAEADKMTRRGATLNVFMLADDKGLVDFVDKLAKRNGGRVFA
                     PSDDKLGDYVVSDYLRARRGRHG"
BASE COUNT          322 a          740 c          693 g          353 t
ORIGIN      
        1 cgaacagtgg gatgacaggc gcgacgatgc cgaacccgac cgccacgctg aacgccaccg
       61 ccgacaggac ggcaacctcg ttcggcaggt cgccgaacac tttcacgcgc agccggtgca
      121 ttggcgtcac gctactccgg cgctacccgt gccgaccgcg cagttatccg tgccgcccgc
      181 ggcgggcccg taggtagtcg ctgacgacgt agtcgccgag cttgtcgtcg gacggcgcga
      241 acacccgccc gccgttgcgc ttggccaact tgtcgacgaa atcgacgagg cccttgtcgt
      301 cggcgagcat gaagacgttg agcgtcgcac cgcgccgagt catcttgtcg gcctccgcga
      361 gggtgaccgc gagagtctcc ggcgagggcg gccagtcgaa ggtgtagtcg ccgctcggga
      421 gcaggtgcgc agttggttcg ccgtcggtga cgacgagcac gaccggctcg tgctcagggt
      481 ggcgatcgag gaatcgtccg gcgatcagta gtgcgtgctg caggttcgtg ccctgcacca
      541 tctcggcgtc caggccggcc agttcggtgg gctgcaaaac ccttgcgtag ttggaaaatc
      601 cgatgacctg caaggcgtct tgcgggaatc gcgtgctgac cagcgagtgc agtgcgagcg
      661 ccgtcgactt ggcgattccc caggtgccgc gcaacgtcat cgaccacgac aggtccacga
      721 gcagacagac cgcggccgag gtgcggcgct cggtctcgac cacctcgaag tcgtcgacct
      781 gcagcttcac tccgccgccc gtttcctcgg acaacgcagg acccgagcga aggatcgcgt
      841 tcgacaccgt gcgcacaacg tccagcggct gttcgtcgcc gaactgccac gcccgcgacg
      901 agccggtcag ctcgccggcc gagccggcgt cgggcacatc gtgatcgcca cgcgtactgc
      961 cggacagcga tgcgaagacc cgtcgcaacg ccgtcgcacc cagccggcgt accgctttcg
     1021 ccgtcagctc cagccgcccg ccgtcccgcc gtaagtagcc ctgccgctcc agctcgcgct
     1081 ccacctcgcg caactggcgc aggtcgtcga cagcctggcg gccgagcgcg cgccggatcg
     1141 cctcctcgtc gacgtcctcg aggctggcgc ccgggtactc ctgcccgagt gtcgactcga
     1201 gttcctcgag atcggcgagc tcttccagcg ccgtcgtcgc gtccgacatg ccgagcggct
     1261 ggttgccgtt catgcgggtg cgtccggtcc aatccagatc gggacgccga ctgcgcagcg
     1321 cgtcgttcaa ccgcgccatc tccgaggcga ggcccatgtc ttccatcgat tgcgccatta
     1381 acgcggcaag ttcgtcgcgc tgctcggcgg tcatcgacgc cagcatccgc tccatcgcgg
     1441 ccgcgcgacg ggcgagcgag tcgacgagct cctcgagatt cgctggctgg tcagggaaaa
     1501 actgcccatg cttggccatg aactgatcga aatcgtcctg cgtgtgttcg ccgcgcgcat
     1561 cggcggccag catctcgttg aggtcggcca tcatgtcctt gacggcctgc atcgcagccg
     1621 ccgattcggg cgacccggac tgcgtcgagt gcatcgcttg cttcatgccg cggaactggc
     1681 tgtcgaggac ctcctggcga aggaggtcct tcagttcctc atacgttgca cgcgcctcat
     1741 cggatcgcca gtcgtaatcg gcaagtgcgc ggatcgcgcg cgccgggtcg ttcggcaacg
     1801 tgtcgagttc agtttcgcgc agccgcgcgt cgtccgacgg atcgggaaac agcgccgcac
     1861 gctcctgccc gaccgctttg tcgagcaacg ctcgcgcctg ctcgagggtg ccgtcgaggc
     1921 gacccttcga tctcagtgcg cggcgctgtt ggcgcacccg gcgcagcatg tcttcgagcc
     1981 cccgaagccc cggcgcacct tggcgcagca ggtcgcgcaa tgcatcggac gtgctggcgc
     2041 cgtcgagcac cgcgtcgccg agttcgtcga gagcctgcgc gatgtcgtac ggcggggcga
     2101 gcgggtcg
//
LOCUS       DATCBJ010000534         2995 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_448122, whole genome shotgun sequence.
ACCESSION   DATCBJ010000534 DATCBJ010000000
VERSION     DATCBJ010000534.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2995)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2995)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2995
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_448122"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..318
                     /locus_tag="VII50_10750"
     CDS             <1..318
                     /locus_tag="VII50_10750"
                     /inference="COORDINATES: protein motif:HMM:NF014591.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HEY5051370.1"
                     /translation="DHEALGVSRSAVKFCDFTDDALDMYVASGEPLDRAGAYAIQGGG
                     SDFVEAVAGRIDTVIGLDVGLALRLLGEIGYPDPLPRTTDIMLTASRQRRPLAPLRTA
                     TRV"
     gene            339..1268
                     /locus_tag="VII50_10755"
     CDS             339..1268
                     /locus_tag="VII50_10755"
                     /inference="COORDINATES: protein motif:HMM:NF013508.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5051371.1"
                     /translation="MRALASHLTGILAVSVLAPLAFVITSCSTEAAQLTVTLTSDFGR
                     SYTPGTTDAEFTIVVHNLGPGNASGVVIHALMPAGFQFADTNYINSDSAARTTPEDAQ
                     VGGSSPQWGVWSLSSPTSPNGLASYASVTINFGVNIVAQPNTYAVAAAVIDDSLTDTV
                     QSRPIQVAVNEAPRLGVTASVSPGTVHPSGQVTYKVTVTNKGTGIAPTVDVLVTLPPV
                     LQYQSTIMPFGGNSSVEALISPVKGAYLVFYGGFELPPQTTLGPGTLIIEFIAQCVPN
                     PGKGVFPIQVSVTDASRDHVGVANAAPLNVFSS"
     gene            1383..2000
                     /gene="rplU"
                     /locus_tag="VII50_10760"
     CDS             1383..2000
                     /gene="rplU"
                     /locus_tag="VII50_10760"
                     /inference="COORDINATES: protein motif:HMM:NF013025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HEY5051372.1"
                     /translation="MYAILQHGGHQYRVAAGDRILVDRIPVEVGSTVTLESVLLIGDG
                     AETDVAKGSPVEASVTATVVAHRRGRKIRVFSYKPKKRHRRTLGYRSQLTELLIDEVG
                     RGKAAAKPKAAAPKRAAAPKAAAKAEPVEAEAEAIAADAEAIAVEAEVVEAEIAVAEQ
                     AVEDAVEAEVAAADKAETEKQAAKKPAAPKRARTPKPTKEPADGA"
     gene            1990..2307
                     /gene="rpmA"
                     /locus_tag="VII50_10765"
     CDS             1990..2307
                     /gene="rpmA"
                     /locus_tag="VII50_10765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007369914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HEY5051373.1"
                     /translation="MAHKKGGGSSRNGRDSKAKRLGVKRHTGEVVLAGSILVRQRGTK
                     ILAGEHVGVGRDHTLFALRTGTVAYDHTGHDRTRANVRAMPIFSVGEMIEEPDEEPVT
                     ATG"
     gene            2336..>2995
                     /gene="obgE"
                     /locus_tag="VII50_10770"
     CDS             2336..>2995
                     /gene="obgE"
                     /locus_tag="VII50_10770"
                     /inference="COORDINATES: protein motif:HMM:TIGR02729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HEY5051374.1"
                     /translation="MDEVDIDVAAGSGGRGAVSFRREKYVPDGGPDGGDGGRGGDVLV
                     IADSTDTTLSGFRERRSFHAEDGRAGGGSKRSGHHGADLTLHVPVGTIVRDGDVVLAD
                     LDRPGAVALAARGGRGGRGNTKFATATRQAPRAGELGDAGEQRHLHLELRLIADIGLV
                     GLPNGGKSTLLAALTGAQPKIAAYPFTTLHPNLGVAELEGGHTLVLADVPGLIEGASH
                     GA"
BASE COUNT          513 a         1018 c         1014 g          450 t
ORIGIN      
        1 gatcacgagg cacttggcgt cagccgttca gcagtcaagt tctgtgattt caccgacgat
       61 gcgctcgaca tgtatgttgc ctccggcgag ccgctcgatc gtgcaggcgc gtacgccatc
      121 cagggcggcg gctcggattt cgttgaggcg gttgccggac ggattgacac ggtcatcggg
      181 ctcgacgtcg ggctcgcgct ccgtctgctc ggcgagatcg gctaccccga tccgctgccg
      241 cggacgacgg acatcatgct gaccgcttcg cggcagcggc gtccgcttgc gccgcttcgc
      301 accgcaacca gggtctgacc gtttcgcgga gcaggcggtt gcgggcactg gcgtcgcacc
      361 tcacgggcat ccttgcggtc tcggtgctgg caccacttgc attcgtgatc acgtcgtgca
      421 gcaccgaggc ggcgcagctc acggtgacac tgacgtcgga cttcggcagg tcgtacacgc
      481 cgggaaccac cgacgccgaa ttcacgatcg tcgtgcacaa cctgggtccg gggaacgcat
      541 cgggtgtggt catccatgcg ctgatgcctg cggggtttca attcgctgac accaactaca
      601 tcaacagcga cagcgcggcc cgcacgacac ctgaggatgc gcaggttggc gggtccagtc
      661 cgcagtgggg tgtgtggtcg ctctcctcgc cgacgtcgcc aaacggtctg gcgagctatg
      721 cgtcggtgac catcaacttc ggggtcaaca tcgtcgcgca gccgaacacg tacgcggtcg
      781 ccgcggcggt gatcgacgac agcctcacgg atacggtgca gagccgtccg attcaagtcg
      841 cggtcaacga ggcgccgcgc ctcggcgtca cagcgtcggt cagccccgga acggtgcatc
      901 cgagcggtca ggtcacctac aaggtcacgg tcaccaacaa agggaccggt atcgccccca
      961 ccgtcgacgt gctggtcacg ctgccgccgg tgctccagta ccagtccacg atcatgccct
     1021 tcggaggcaa ctcgtcagtc gaggcgctga tatcaccggt caaaggcgcc tatctcgtgt
     1081 tctacggcgg cttcgagtta cccccgcaga ccacgctcgg cccaggaacg ctgatcatcg
     1141 agttcatcgc gcaatgcgtc cccaacccgg ggaagggcgt tttcccgatt caggtttcgg
     1201 tcaccgacgc ctcgcgcgac cacgtcggcg tcgccaacgc ggcgccgctc aacgtcttca
     1261 gttcctgagt gcatcccgtc cccgagggcc tgtcaccgcg ctcacgtggt aacctcgcgt
     1321 atcgcgaccg gtaacagccg gcgttcctca gtggagtgaa cccgctccgg cgagagaggc
     1381 atatgtacgc aatccttcaa cacggcggtc atcaataccg tgtcgccgcg ggcgaccgga
     1441 tccttgtcga ccgcattccc gtcgaggtcg gctcgaccgt gaccctggag tccgtcctgc
     1501 tgatcggtga cggtgctgag accgatgtgg ccaagggatc gccggtcgag gcctcggtca
     1561 ccgcgacggt tgtcgcgcat cgacggggcc gcaagatccg cgtcttctcg tacaagccga
     1621 agaagcgcca ccggcgcacg ctcgggtacc gcagccagct gaccgagctg ctcatcgacg
     1681 aggtcggccg cggcaaggcc gccgccaagc cgaaggcagc cgcgccaaag cgtgcggctg
     1741 caccgaaggc cgccgcaaag gcggaaccgg tcgaggcgga ggctgaggcc atcgcggcgg
     1801 acgccgaggc gatcgcagtg gaagccgagg tggtcgaagc ggagatcgcc gtcgccgagc
     1861 aagcggtcga ggatgctgtg gaggccgagg tcgccgcggc cgacaaggcg gaaaccgaga
     1921 agcaggcggc gaagaagccc gcggctccga agcgagccag gacgcccaaa cccacgaagg
     1981 aaccagccga tggcgcataa gaaaggtggc gggagctccc gcaacggccg cgattcgaag
     2041 gcgaagcgcc tcggcgtgaa gcgccacacc ggtgaggtcg tgctcgcggg cagcatcctg
     2101 gtccgccagc gcggcaccaa gatcctcgcg ggagagcacg tcggcgtcgg ccgcgatcac
     2161 acgctcttcg cgttgcgcac gggcacggtt gcctacgatc acaccggcca cgacaggacg
     2221 cgcgccaacg ttcgcgcaat gccgatcttc tcggtcggcg agatgatcga agagcccgac
     2281 gaggagccgg tcacagcgac cggctgagcg tacggccact cgtggctccc ccttcatcga
     2341 cgaggtcgac atcgatgtgg cggcgggatc aggcgggcgg ggagctgtca gctttcgccg
     2401 cgagaagtac gttcccgacg gcggtccgga cggaggcgat ggagggcgtg gcggtgacgt
     2461 gctcgtcatc gccgattcga ccgacaccac gctctcaggc tttcgtgaac ggcgttcctt
     2521 ccacgcggaa gacggccgcg ccggcggcgg cagcaagcgc tccggccatc acggcgccga
     2581 tctgacgctg cacgtcccgg tggggacaat cgtccgcgac ggcgacgtcg tgctggccga
     2641 cctcgaccgc ccgggcgctg tagcgctcgc agcgcgcgga gggcgcggcg gacgcgggaa
     2701 caccaagttc gccaccgcca cgcggcaggc tccgcgcgcc ggggagctcg gtgacgcggg
     2761 cgaacagcga cacctgcacc tcgagctgcg gctcatcgcc gacatcggcc tggtggggct
     2821 gccgaacggg ggcaagtcga cgctgctggc ggcgctcacc ggcgcgcagc cgaagatcgc
     2881 cgcgtatccg ttcaccacgc tgcatcccaa ccttggcgtt gccgagctcg agggcggaca
     2941 cacgctcgtc ctggccgacg tcccgggcct catcgagggc gccagccacg gcgcc
//
LOCUS       DATCBJ010000535         2963 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_234699, whole genome shotgun sequence.
ACCESSION   DATCBJ010000535 DATCBJ010000000
VERSION     DATCBJ010000535.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2963)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2963)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2963
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_234699"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..226)
                     /locus_tag="VII50_10775"
     CDS             complement(<1..226)
                     /locus_tag="VII50_10775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5051375.1"
                     /translation="MRGLWRTARAKLVITALAVGAAVVCVIALSGSSALPTRVAKARP
                     GSAIAQGTATTYRPLRINPPETAVERTVNQT"
     gene            complement(226..537)
                     /locus_tag="VII50_10780"
     CDS             complement(226..537)
                     /locus_tag="VII50_10780"
                     /inference="COORDINATES: protein motif:HMM:NF039351.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6112 family protein"
                     /protein_id="HEY5051376.1"
                     /translation="MITQFASARLMASGVTVTPNSTAIPGINTLSTLVGSLMAFGLVL
                     SLAGFVGCAIVMAIGHHSGNPQWSTRGKMGLLVCLVAALLIGGSDLLINTFYNLGTTL
                     S"
     gene            complement(598..1263)
                     /locus_tag="VII50_10785"
     CDS             complement(598..1263)
                     /locus_tag="VII50_10785"
                     /inference="COORDINATES: protein motif:HMM:NF013073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HEY5051377.1"
                     /translation="MKKVLFLAAPLAWFVAALLGLVILFSTVGGTAQATACTLSALST
                     TKTTSSKASVTTTTTTLSSNANCGAAARSTLPTSPVVNNGLPTNYVIPADATAAETAA
                     ITYALAQLGKLYLWGGTGPAAFDCSGLTMMAWASAGVTLLHYTGDQINEGTAVSSYAE
                     IAPGDLVLVPGSDGTIANPGHVGIYLGDGMVESAVDVQYGVIVQPYSYFISGGLSGIR
                     HVG"
     gene            complement(1260..1982)
                     /locus_tag="VII50_10790"
     CDS             complement(1260..1982)
                     /locus_tag="VII50_10790"
                     /inference="COORDINATES: protein motif:HMM:NF016119.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class F sortase"
                     /protein_id="HEY5051378.1"
                     /translation="MSILRQTAVATAAAGLVLAAVAVVSHPLASAPLVKHPFALKSAA
                     TLPVQPAGARVVADHLVIPSLHVEAFVRTTSIVDHELVIPGDVHDVGEWGRKVNAGTT
                     ILAGHVNWVGQGLGALGGLANIAPGATVYLSNRAGVVSVWSVVSLRAVSKMDLPETLF
                     ASGGTRRLVLVTCGGAFDATTHQYADNVLATAVPAQTRAMEIATRTNVAGQQAVSVAQ
                     DFPPPWPAPRSRNPSLEAAERP"
     gene            complement(1987..>2963)
                     /locus_tag="VII50_10795"
     CDS             complement(1987..>2963)
                     /locus_tag="VII50_10795"
                     /inference="COORDINATES: protein motif:HMM:NF037639.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
                     /protein_id="HEY5051379.1"
                     /translation="YSVTGPSKTGTGCYAWADSLTLDPSGASATSSPLEASEQTLVAV
                     PVTFIKTSTTDPTNTPVAGAVFSLASASGNILATGLVSGSGPVAIDVPGLYFMQGATY
                     ELIETRAPSGYYIPVSNVASFTIPASATTFNVITTDPPIPLMRPASVVSQIRARYLTP
                     GGHVADSIDVTVDDGEPGTITGTLYGPVAPPASMKCSDLTLAQWEVAPPQVFTAVLDA
                     NGTGTVMGPVPTQAGCYGWAEKVTLTASGATASTPPTSANEQAFASVKGSGNGVGGTG
                     SGHSTTTIDTGGPVLAGLNKTLLASGLALLALAMLLVDLDRKRRNARG"
BASE COUNT          499 a          957 c          977 g          530 t
ORIGIN      
        1 atgtttgatt gaccgtccgc tcgactgccg tctcgggcgg gttgatccga agcgggcgat
       61 acgtggtcgc cgtaccttgt gcgatggcgg acccgggtcg tgcttttgcc actctcgtcg
      121 gtaacgccga cgaacccgag agggcgatca cgcacaccac cgccgccccg accgcgaggg
      181 ccgtgatcac cagtttcgcg cgcgcagttc tccagaggcc tctcatcagc tgagggtcgt
      241 gccgaggttg tagaacgtgt tgatcaacaa gtccgagccg ccgatgagca gggccgcgac
      301 gaggcacacg agcagtccca tctttcctct cgttgaccac tgcggattac cactgtggtg
      361 gccaatcgcc ataacgatcg cgcaccccac gaacccggcc agcgagagca cgagaccgaa
      421 ggccatgagt gaacccacga gcgtcgacaa agtgttgatg ccgggtatcg ccgtgctgtt
      481 cggcgtcacc gtcacgccgc tggccatgag ccgggctgat gcgaattgtg taatcactgt
      541 tgctcctctg tttggatgat tttcttctgc gcgaatttcg cgcgcgtccg gatccgctta
      601 acccacatgg cgaattcccg acagcccgcc gctgatgaaa tacgagtacg gctggacgat
      661 cacgccgtac tgcacgtcga ccgccgactc gaccatcccg tcgccgaggt agatcccgac
      721 gtggccgggg ttcgcgatag ttccgtcgct gccgggcacg aggacgaggt cgccgggtgc
      781 tatctccgcg tacgagctca cggcggtacc ttcgttgatc tgatcgccgg tgtagtgcaa
      841 gagcgtcact ccggcactcg cccaggccat catggtgaga cccgagcagt caaaggccgc
      901 gggaccggta ccgccccaga ggtacagctt gccgagctgg gccagggcgt aggtgatcgc
      961 cgccgtctcg gcggccgtcg catcggcggg aatcacgtaa ttcgttggca gtccgttgtt
     1021 gacgaccggt gaggtcggca aggtggagcg cgccgccgcg ccacagttcg cgttcgagga
     1081 aagcgtcgtt gtagtcgtgg tgactgatgc cttggaagaa gttgtcttgg tcgtggagag
     1141 cgcggacaat gtgcaggcag tggcctgggc cgtcccgccc accgtgctga acagaatgac
     1201 gaggcccagg agagctgcca cgaaccacgc gaggggcgcc gcgagaaaga gcaccttctt
     1261 cacggtcgct ccgccgcttc gagtgaaggg ttccgagagc gtggcgcagg ccaaggggga
     1321 ggaaagtcct gcgccacgct cacggcttgt tgccccgcca cattggtgcg ggttgcgatc
     1381 tccattgcgc gcgtttgcgc gggcacggcg gtagcgagaa cgttgtcggc gtactggtgg
     1441 gtcgtcgcgt cgaaagcgcc gccgcaggtg accagcacca gtcgacgggt tccgccgctc
     1501 gcgaacagtg tctcgggcag gtccatcttc gagaccgccc gcagacttac gacgctccac
     1561 acgctcacta cacccgcccg gttggacaag tacaccgtgg ccccgggggc aatattggcg
     1621 aggcccccca gtgcaccgag gccttggccc acccagttga cgtgtcccgc gaggatggtg
     1681 gtgcccgcgt taaccttgcg cccccactcg cccacgtcgt gcacgtcgcc cgggataacg
     1741 agctcgtgat ccacgatcga cgtggtgcgc acgaacgctt cgacgtgcag cgacgggatc
     1801 accaggtggt cggcgactac cctcgcgccg gcgggctgta ccggcagcgt ggccgcgctc
     1861 tttagtgcga agggatgctt caccagtggg gcactcgcga gggggtggct caccaccgcc
     1921 accgccgcca gcaccaggcc cgccgccgca gtggcgactg ccgtttgccg aaggatgctc
     1981 atggctctag cccctggcgt ttcgacgttt gcgatccagg tcgaccaaca acatggccag
     2041 cgccaggagt gccagaccgc tcgccagcaa tgtcttgttc aatccggcca acaccggacc
     2101 cccggtgtcg attgtggtcg tggagtgacc cgagcccgtt ccacccacgc cattgccgga
     2161 gcccttgaca ctggcgaacg cttgctcgtt cgccgacgtg ggcggcgtgc tcgcggttgc
     2221 ccccgaggcc gtgagcgtca ctttttctgc ccagccgtag catccggcct gcgtcggcac
     2281 tggccccatc actgtgcccg tgccattggc gtcgagcacg gcggtgaaga cctgcggggg
     2341 cgcgacttcc cactgagcga gcgtgaggtc actgcacttc atcgaagccg gcggcgccac
     2401 cggaccgtac agcgtgccgg tgatcgttcc gggctctccg tcatcgacgg tgacgtcgat
     2461 gctgtcggcc acgtggccgc cgggagtcaa gtaccgcgcg cgaatctgcg agacaaccga
     2521 ggccggtctc atcagcggga taggtggatc agtcgtgatg acgttgaacg tcgtcgcgct
     2581 cgcagggatc gtgaacgagg ccacgttgct gaccggaatg tagtagccgc ttggggcccg
     2641 ggtctcaatc agttcgtagg tcgcgccctg catgaagtac agaccgggga catcgatcgc
     2701 tacgggcccg gatccactca cgagacccgt cgccagaatg ttgccgctgg cgctcgccaa
     2761 tgagaacacg gcgccggcaa cgggcgtgtt ggtcgggtcc gtggttgacg tctttatgaa
     2821 ggtcaccggg accgccacca acgtctgttc gcttgcctcg aggggcgacg acgtggcact
     2881 cgcacccgac gggtcgaggg tcaggctgtc cgcccaggcg tagcagccgg taccggtttt
     2941 actcgggccg gtgacgctgt agg
//
LOCUS       DATCBJ010000536         3525 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_392035, whole genome shotgun sequence.
ACCESSION   DATCBJ010000536 DATCBJ010000000
VERSION     DATCBJ010000536.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 3525)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3525)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3525
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_392035"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..525)
                     /locus_tag="VII50_10800"
     CDS             complement(<1..525)
                     /locus_tag="VII50_10800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="S26 family signal peptidase"
                     /protein_id="HEY5051380.1"
                     /translation="MATEVAVEVPALAGPQPRPASNVTLFGGFVGRTWLWFLGGCLVV
                     TLVPLLFGWRPYVVESSSMAPRIKVGDVILAAPEHNPQKLLGRVTVFHDPDPGRIGSI
                     KSHRVVAINSDGTLTTKGDANQSVDSVHVKVSAVVGLGRLLVRYVGLPLIWVQKGQWL
                     KFALLLASIWFAALL"
     gene            complement(632..1327)
                     /locus_tag="VII50_10805"
     CDS             complement(632..1327)
                     /locus_tag="VII50_10805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5051381.1"
                     /translation="MTKDIKSSPISTEQLAKSTTIWEPSMSKQSDRRRRVVRATAPAA
                     GLLAAGLLVWQGSYAAFSATTVDTGNAWASGSLVLQNNGGTGTYAAATSASFGGNNLK
                     PGSGNTTCLTVKSTGTAPGNLAMYESSITDSSPSLGAQIQLTITAAPVATDVLANCTG
                     FPASGTTAIATNQPLSTFAATYATGVPVAVAAGTVLEAYKVVWTFATTGTNPGDSALM
                     GKTVTSGFTWELQ"
     gene            complement(1497..2126)
                     /locus_tag="VII50_10810"
     CDS             complement(1497..2126)
                     /locus_tag="VII50_10810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5051382.1"
                     /translation="MSHPVSVERKRHLIRIIAPAVGLLAAGLLVWQGSYAAFSATTVD
                     TGNAWAAGTLVLTNNGGTAVYSAATTATFGGSNLKPGSTGTTCLTVKSTGTAAGTLAM
                     YRSALADSAPSLGAQIQLTVTEGVPATDVQANCVGFPAAGVTTVATNQPLSTFPSSFA
                     TATGTTGVAAGTVLEAYKVVWTFVTTGTNPGDTALMGKTVTAGFTWEIQ"
     gene            complement(2280..3239)
                     /locus_tag="VII50_10815"
     CDS             complement(2280..3239)
                     /locus_tag="VII50_10815"
                     /inference="COORDINATES: protein motif:HMM:NF013961.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase-like domain-containing protein"
                     /protein_id="HEY5051383.1"
                     /translation="MSSASPAAGTATLRAATGSDPSDPKWTAVEPGLPARVRDLAAQL
                     TASAPTRYDAVSAVDDYLQTHEVYDLTSPIPAPGQDAVDDFLFVSHSGFCEQFASAAV
                     IMLRSTGIPARLVTGYSRGDLRNEPGERVMRGTDAHAWIQVWYPGVGWVNSDPTAAAL
                     LPAAAAPTSVASPDRHPLPLAAALRIMPGRRTGWLVAIVGFIVLVACAGLLIGALKRR
                     RPGALRPSVTQEPRPGDGPVLQAYMRLDAALGGQAREPEQTLREVARHLDTAGCSRAE
                     LTVALDCLERECYAIVPPAPTEVAVAVDVFDRLHASLNGVLVP"
     gene            3254..>3525
                     /locus_tag="VII50_10820"
     CDS             3254..>3525
                     /locus_tag="VII50_10820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5051384.1"
                     /translation="MLSPRGLDVVMAPGTPTAYSGPGEMLAGADGTAPLTAAGATSPW
                     TYQVSSASPTADAVTLRAATGSDPGDPKWTTVEPGLPGRVRDLAAQL"
BASE COUNT          602 a         1152 c         1211 g          560 t
ORIGIN      
        1 caacagcgca gcgaaccaga tagacgcgag caacagcgca aacttcagcc actggccctt
       61 ttgcacccag atcagcggta gcccgacgta cctgaccagg agccggccga gaccgacaac
      121 cgccgacacc ttcacgtgca cactgtcgac cgactggttg gcgtcgccct tggtcgtgag
      181 cgttccgtca gagttgatcg caacgacgcg gtgcgacttg atgctcccga tccggcccgg
      241 gtcagggtcg tgaaacaccg tgactcgacc gagcagcttc tgcggattgt gttccggcgc
      301 agcgaggatc acatcgccga ccttgatccg cggcgccatc gacgaggact cgacgacgta
      361 gggccgccag ccgaacagca agggcaccaa cgtgacgacc aggcagccgc ccaggaacca
      421 cagccacgtg cggccgacga acccaccgaa cagcgtgacg ttgctggccg gacgcggctg
      481 cggtcctgct agcgcaggta cctccaccgc tacctcggta gccaccacac gtcccttcag
      541 atgttcggca cgaacagccg tgagctcccg ggccccttgc ggggccccgg gagccggcgg
      601 tttagttcga cgggttagtg atcagtcgct cctactggag ttcccacgtg aagccggagg
      661 tgacggtctt gcccatcagg gcggagtcgc cggggttggt gccggtggtg gcgaaggtcc
      721 acacgacctt gtaggcctcg aggacggtgc ctgcggcgac tgcgacgggg acgccggtcg
      781 cgtacgtggc cgcgaacgtc gacagcggct ggttcgtcgc gatcgccgtc gtaccggagg
      841 ccgggaagcc cgtgcagttc gccagcacgt cagtcgcgac cggagccgcg gtgatcgtga
      901 gctggatctg cgcaccgagc gaggggctcg agtcggtgat cgagctctcg tacatggcca
      961 ggttgccggg agcagtgccg gtgctcttga cagtgaggca ggtggtgttg cccgaaccgg
     1021 gcttcaagtt gttgccacca aacgacgcac tcgtcgcagc ggcgtaggtg ccggtgccgc
     1081 cgttgttctg cagcaccagc gagccggacg cccacgcgtt gccggtgtcg acggtggtgg
     1141 cggagaaggc ggcgtagctg ccctgccaca cgaggagacc ggcggccaac aggccggcgg
     1201 ccggggcggt tgcacggacg acgcggcgcc ggcggtcaga ctgcttggac attgatggct
     1261 cccagatagt ggtggacttg gcgagttgtt cggtggaaat gggcgacgac ttgatgtctt
     1321 tggtcacgat ctggccggtc ggttgatggc agcggtcacg tggcccaacg gcccgcgcgg
     1381 tcttgggcta atcgggttac gcacaccgga ctagagtcgg cgcaataccg gacgaaatcg
     1441 ggcaagaaaa gagcccctca cgtgtcctga ggggctcttt ccggacaact agcgttctac
     1501 tggatttccc acgtgaagcc cgccgtgacc gtcttgccca tcaaagccgt gtcgccggga
     1561 ttcgtgcccg tggtcacgaa tgtccagacg accttgtagg cctccaggac ggtgcccgcg
     1621 gcgacccccg tggtgccggt cgctgtcgca aagctgctcg ggaacgtcga caacggctgg
     1681 ttggtcgcca ccgtcgtaac gcctgcggcc gggaagccga cgcagttcgc ctgcacatcg
     1741 gtcgccggca caccttcggt gacggtcagc tggatctgcg cgcccaacga cggcgcggag
     1801 tctgcgagcg cgctgcgata catggccagt gtgccggcgg cggtaccggt gctcttgacg
     1861 gtcagacacg tcgtcccggt cgaaccaggc ttcaggttcg agccaccgaa cgtcgcagtc
     1921 gtcgccgcgg agtagaccgc ggtgccgccg ttgttggtca acaccaacgt gcctgcggcc
     1981 cacgcgttac ccgtgtcgac agtagtggcg gagaaggcgg cgtagctgcc ctgccacacg
     2041 aggagaccgg cggccaacaa gccgactgcc ggggcgatga tccggatcag atggcgcttg
     2101 cgctcgacag atacagggtg cgacacggga gggctccaaa gtccgggggg acgtcggagt
     2161 tcgtgttcgg cgcgactttg tgattccttg actgccgcgg ggcatttctg tctaaccgga
     2221 tgaggacaga ctcggtcgcg cggtagctca aaccggacgc tgcactcacc atgcccaatt
     2281 cagggaacca gaacgccgtt caatgaagcg tgcaggcggt cgaacacgtc gacggccacg
     2341 gcgacctcgg ttggtgcggg cggcacgatc gcgtagcact ctcgctcgag acaatccaac
     2401 gccacagtca attccgcgcg tgagcaacct gcggtgtcga gatgtcgcgc cacctcgcgc
     2461 agggtctgtt caggctcgcg tgcctgccca cccaacgccg cgtcgagacg catgtacgcc
     2521 tgcaggactg ggccgtcacc gggtcgcggc tcttgggtga cggacggcct cagcgcgccc
     2581 ggccgacgcc gcttgagggc accgatgagc agtcccgcgc aggcaacaag cacgatgaaa
     2641 ccgacgattg caaccagcca acccgtccgg cggccgggca tgatccgcag cgcggcagcc
     2701 agtggcagcg ggtgccgatc cggtgaagca actgacgtcg gcgccgcagc ggcgggcagc
     2761 agggccgccg ccgtcggatc gctattgacc caaccaacgc cggggtacca cacctggatc
     2821 cacgcgtgcg cgtctgtccc gcgcatgaca cgctctccgg gctcgtttcg gaggtcaccc
     2881 cggctgtagc ccgtgaccag ccgcgccggg atgcctgtgc tgcgcagcat gatgacagcg
     2941 gcggacgcga actgctcgca gaacccgcta tgggaaacaa agaggaaatc gtcaacggcg
     3001 tcctgcccgg gcgcaggtat cggcgacgtc aggtcgtaga cctcgtgcgt ctggagatag
     3061 tcgtccactg cgctcaccgc gtcgtaacgc gtgggcgcgg acgctgtcag ttgggcggcg
     3121 aggtcgcgaa cccgcgcggg cagacccggc tcgaccgccg tccacttcgg gtcgctcgga
     3181 tcgctgcccg tcgcagcgcg caaagtcgca gtgccggccg ccggactcgc cgacgacacc
     3241 tgatacgtcc agggtgctgt cgcctcgcgg gctcgacgtc gtcatggcgc cgggtactcc
     3301 gactgcgtat agcgggcctg gcgagatgct cgctggagct gatggcactg cgcccttgac
     3361 cgcggcgggc gcgacatcac cttggacgta tcaggtgtcc tcggcgagtc cgaccgcgga
     3421 tgccgtgacg ctgcgcgctg cgacgggcag cgatccgggc gacccgaagt ggacgaccgt
     3481 ggagccgggt ctgcccgggc gtgttcgcga cctcgccgca caact
//
LOCUS       DATCBJ010000537         2208 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_456179, whole genome shotgun sequence.
ACCESSION   DATCBJ010000537 DATCBJ010000000
VERSION     DATCBJ010000537.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2208)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2208)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2208
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_456179"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..764
                     /locus_tag="VII50_10825"
     CDS             <1..764
                     /locus_tag="VII50_10825"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY5051385.1"
                     /translation="VHSFFSVLRRDVTVTGRELPIFLAQVILQPLGLLFIFGKVLGSI
                     GLTTPGYAHLLFPGIVALTVTLTGIQSTALPLVLEFSFSREIEDRLLAPLPTWAVALE
                     KMLFASMRALLAALVMFPVGIWVLGSIPWHAGAIWLVAVVMILGALAGSAIGLTLGTL
                     VPPNRIQIMFALILTPMLFTGCSQYPWPSLSHLRWFQVVTAFNPMTYASEGMRAALTP
                     SVHHMYPWVCVLGLALSVAVFTTIGIRGFLRRAID"
     gene            complement(909..1241)
                     /locus_tag="VII50_10830"
     CDS             complement(909..1241)
                     /locus_tag="VII50_10830"
                     /inference="COORDINATES: protein motif:HMM:NF016854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TfoX/Sxy family protein"
                     /protein_id="HEY5051386.1"
                     /translation="MAYDEVLANRLRECLQDEAGVNEKAMFGGLAFLVNGNMAVSASG
                     QGGLLLRVDPTQTDALAAKPHARRFEMRGREMDGWLRVDADGVKTSRDLKKWVAIGVS
                     YARSLPPK"
     gene            1359..2111
                     /locus_tag="VII50_10835"
     CDS             1359..2111
                     /locus_tag="VII50_10835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009079246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEY5051387.1"
                     /translation="MTPWQALLLVVAGVGGGLSGSIAGLASLVSYPALLAVGLAPVTA
                     NVTNTVALVFSSVGSVAGSRPELRGQRSRVRQLGVVAILGGVAGGTLLLLTPRDSFER
                     VVPWLIGLGSLTILWPRRPVPHPDVPHPPASRLLVVGIFLIGVYGGYFGAAAGVLMVA
                     LLLIATPETLARCNALKNVLLGLANAVAAVIFAIFGPVHWEAVIPLAAGLLVGGRIGP
                     IVVRHSSARALRVLIALAGIGLAVHLGLDAYR"
BASE COUNT          284 a          728 c          722 g          474 t
ORIGIN      
        1 cggtgcactc gttcttcagc gtgttgcgac gcgacgtcac tgtgaccggt cgcgagctgc
       61 cgatcttcct tgcgcaggtg atcctgcagc cgctgggatt gctcttcatc ttcggcaagg
      121 ttctgggctc gatcgggctg accactccgg gatatgcgca cctgttgttt cctggcatcg
      181 tcgcgttgac agtgacgttg acgggaattc agagcaccgc tctgccactg gttctggagt
      241 tctcgttcag ccgcgagatc gaggaccgat tgctcgcgcc gttgccgacg tgggcggtcg
      301 cgctcgagaa gatgctcttc gcgtcgatgc gcgcgctgct tgctgcgttg gtgatgttcc
      361 cggtcggtat ctgggtgctc ggctcgattc cttggcacgc cggcgcgatc tggctcgtcg
      421 ctgtggtgat gattctcggc gcacttgcgg ggtctgcgat cgggctcacg ctcggcacgt
      481 tggtgccgcc gaatcggatc cagatcatgt tcgcgctgat tctgacgccg atgctgttca
      541 ccggctgtag ccaatatccg tggccatcgc tttcgcatct ccgctggttc caagttgtca
      601 ccgccttcaa cccgatgacg tacgcgagcg aggggatgcg agctgcgctg acgccgtccg
      661 tgcatcacat gtacccgtgg gtttgtgtac ttggtctcgc gttgtcggtc gcagtgttca
      721 ccacgatcgg tatccgcgga ttcttgcgcc gcgccatcga ctgatgctca accgcgggcc
      781 ttcggcggac acgatgtcgg ccggcagcgt ggccggtatg agcgccagta gctgctgcac
      841 tgtcggcggc ggcccctggc ggcgatgggg agtgtggggt cgacgcccga acacgtgacc
      901 aacgagtctt acttcggtgg cagtgatcgg gcgtagctga cgccgatggc gacccacttc
      961 ttcaggtccc gcgaggtctt tacgccgtcg gcgtcgacac gtagccagcc atccatctcg
     1021 cgcccgcgca tttcgaagcg ccgcgcgtgt ggtttggctg ccagggcatc tgtctgcgtc
     1081 ggatccacgc gcagtagcag gccaccttga ccgctcgcgc tgaccgccat gttgccgttc
     1141 accaagaagg cgagtccgcc gaacatggcc ttctcgttga ccccggcttc gtcttgcagg
     1201 cactctcgaa gccggttcgc cagcacctcg tcgtacgcca tgagcttgag tatctacgat
     1261 cgagccatga ccgcgaccgg ccgggccccc gaacgcagag ccgacgacga ggtccacgcc
     1321 gacatcgtcc gcgagcgcat tgacgggttg cggggcgagt gacgccatgg caggcgttgc
     1381 tgcttgtcgt cgctggcgtc ggtggaggct tgtccggcag catcgcgggt ctcgcgtcgc
     1441 tggttagcta ccccgcgctg ctcgccgtcg gtctcgcgcc ggtcacggcg aacgtcacaa
     1501 acaccgttgc gttggtcttc agcagtgtcg gatcggtggc aggttcgcgg ccggaactgc
     1561 gcggtcagcg ctcccgggtt cgtcagctcg gcgtggtcgc catccttggt ggcgtagccg
     1621 gaggcacact cctgctgctc acgccgcgcg actcgtttga gcgcgtcgtg ccgtggctca
     1681 tcgggctcgg ctcgctcacg atcctgtggc cccggcgtcc ggtgccccat cccgatgtcc
     1741 cgcatcctcc cgcctcacgg ctgctcgtcg tcggcatctt cctgatcggc gtctacggcg
     1801 gctacttcgg ggctgctgcc ggggtgctga tggtcgcact gctgttgatc gcgacgccgg
     1861 aaacactcgc gcgctgcaat gccttgaaga acgtgcttct cggcctggcc aacgccgttg
     1921 cagccgtcat tttcgccatc ttcgggccgg tgcactggga agcggtgatt ccgctcgctg
     1981 ccggcctgct ggtcggcggg cgcatcggac ctatcgtcgt ccgacactcg tccgcccgcg
     2041 cgcttcgcgt cctgattgcg ctcgcgggca tcgggctggc ggtccacctc ggactcgacg
     2101 cctaccgttg aaccggctag gagggtcgcg gtggcggcac ccattgcggc atcgtctgca
     2161 acaccgagcc gttgcgttcg atccgcaggc tggcaggccc ctcaccgg
//
LOCUS       DATCBJ010000538         2066 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_224430, whole genome shotgun sequence.
ACCESSION   DATCBJ010000538 DATCBJ010000000
VERSION     DATCBJ010000538.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2066)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2066)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2066
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_224430"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..779)
                     /locus_tag="VII50_10840"
     CDS             complement(<1..779)
                     /locus_tag="VII50_10840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019310943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA helicase domain-containing protein"
                     /protein_id="HEY5051388.1"
                     /translation="MADVETELVREQQYVDRLYSRLDELRDRTERELIAVRATAPSGT
                     HQNRSERDSFAGLYEQRLSQLRAVEDRLVFGRFDTVEEGRRYVGRIGLSDEDRHQLLI
                     DWRAPAARLFYQATAASPGDVVRRRHLTTRNRAVTAIEDEVLDLDGLEASDLSTLSGE
                     GALLAALNAKRTGRMGDIVATIQAEQDDVIRAELPGVLVVQGGPGTGKTAVALHRAAY
                     LLYTHRERLSRSGVLLVGPNQTFLRYIEKVLPSLGETGVVMV"
     gene            939..1886
                     /locus_tag="VII50_10845"
     CDS             939..1886
                     /locus_tag="VII50_10845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020662254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY5051389.1"
                     /translation="MIEARDLSKRYGDKLAVDHLSFTVQPGTVTGFLGPNGAGKSTTM
                     RLLLGLDRPDTGSATIDGRHYRDLAEPLRVVGALLEARAVHSGRSAYNNLLVLAQTQG
                     LPHKRVDEVIELVGLTSVAKKRVRGFSLGMGQRLGIAAALLGNPDVLILDEPVNGLDP
                     EAILWIRNLLKRLASEGRTVFVSSHLMNEMAVTADHLIVIGRGKLMADCTTEDFIERS
                     SEKSVLVKSPDVTRLAELITADGGLIAKTDGDEVTVRNLAAPRIGHLAASAGIELHEL
                     APQAASLEEAFMELTRGSAEYEGSIDGATAPLLPEGGAA"
     gene            1883..>2066
                     /locus_tag="VII50_10850"
     CDS             1883..>2066
                     /locus_tag="VII50_10850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5051390.1"
                     /translation="MTTTTVSSPAIHTASTGVRTVRLLRSEWTKLVTVRSTLWSLLLL
                     VILTPGLTALFAWLNEH"
BASE COUNT          370 a          691 c          640 g          365 t
ORIGIN      
        1 accatcacga cgccggtctc gcccagcgac ggcagcactt tctcgatgta tcgcaggaac
       61 gtctggttgg gtccgaccag cagcacgccg cttcgggaca gccgctcgcg gtgcgtgtag
      121 aggaggtacg cggcgcgatg cagtgcgacc gccgtcttcc cggtacccgg accgccctga
      181 accaccagca ccccaggcag ttctgccctg atgacgtcgt cctgctcggc ctgaattgtc
      241 gcgacgatgt cgcccatgcg gccggttcgc ttcgcgttga gcgcggcaag cagcgcaccc
      301 tcacccgaca gcgtcgaaag gtcactcgcc tcgagcccgt cgagatcgag cacctcgtcc
      361 tcgatcgccg tcactgcacg attccgcgtc gtcaggtggc ggcgccggac cacgtcgccc
      421 ggagaggcag cagttgcctg gtagaagagc ctggccgccg gcgcccgcca gtcgatgagc
      481 aactggtgcc tgtcctcatc tgacaatccg atgcgcccca catatcggcg tccctcttca
      541 accgtgtcga accgcccgaa gaccagccga tcctccaccg cacgcagctg agacaggcgc
      601 tgctcatata agccggcgaa ggaatcgcgc tcggagcggt tctgatgggt acccgaggga
      661 gcagtagccc ggacggcgat cagctcgcgt tctgtgcgat cgcgcagctc atcgagcctg
      721 gagtacaggc ggtcgacata ctgctgctca cgcacgagtt cggtctctac gtcggccacc
      781 gaaccccttc cgatcgtgca tcaccgccga tcgtgcggcg tctaccctac ctgtcgggcg
      841 ccagccatgc gtacgtgaca tttgtctgag gcgatccggg tatgggcccg atacgttggg
      901 caatggattc ctcaccgcag ctaaggaggc aggcaggcgt gatcgaggcg cgcgatctca
      961 gcaagcggta cggcgacaag ctcgcggtcg atcacctctc atttacggtg cagccaggca
     1021 ccgttaccgg gttcctcggc ccgaacggcg ccggcaaatc gacgacgatg cggctccttc
     1081 tcggactcga ccgccccgat accggcagcg caaccatcga cggtcggcac tatcgcgacc
     1141 tcgccgaacc gctacgggtc gtgggcgcgc tacttgaggc gcgagccgtt cacagtggtc
     1201 gcagcgccta taacaacctg ctcgtattgg cgcagaccca aggcttgcct cacaagcgcg
     1261 tggacgaagt catcgagctc gtcggtctca ccagtgtcgc caagaagcga gtccgcgggt
     1321 tttcgctcgg catgggccag cggctgggta tcgccgcggc acttctcggc aaccccgatg
     1381 tcctcatact cgatgagccg gtcaacgggc tcgacccaga agcgatcttg tggatccgca
     1441 acttgctcaa gcgactcgcg tccgagggcc gaacggtctt cgtctcgagt cacctgatga
     1501 acgagatggc cgtcacggcc gaccatctga tcgtgatcgg ccgcggcaaa ctgatggccg
     1561 actgcacaac cgaggatttc atcgaacgaa gctccgagaa gtcggtgcta gtgaagtctc
     1621 cggacgtgac tcggctggct gagctcatca ccgccgacgg tgggctcatc gcgaagacgg
     1681 acggcgacga ggtgaccgtt cgcaatcttg ccgcaccgcg gatcgggcat cttgctgcaa
     1741 gcgcggggat cgagcttcat gaactggctc cgcaggcggc atcgctagag gaggcgttca
     1801 tggaactgac gcggggcagc gccgaatatg agggctcgat cgacggcgct accgctccgt
     1861 tgttgcccga aggcggtgca gcatgacgac gacgaccgtt tcctcgccgg ccatccacac
     1921 ggcgtccacc ggagtccgca cggttcgctt gctgcgctcg gagtggacca aacttgtcac
     1981 cgtgcgttcg acgttgtggt cgctgctgct gctcgtgatc ctcacaccag ggctcaccgc
     2041 actgttcgct tggctgaacg agcaca
//
LOCUS       DATCBJ010000539         2614 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_414683, whole genome shotgun sequence.
ACCESSION   DATCBJ010000539 DATCBJ010000000
VERSION     DATCBJ010000539.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2614)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2614)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2614
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_414683"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..345
                     /locus_tag="VII50_10855"
     CDS             <1..345
                     /locus_tag="VII50_10855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbQ family protein"
                     /protein_id="HEY5051391.1"
                     /translation="DTVAAFCRGLGDGLLNVFVPHATAGIALIEIGSGSEHDLGATID
                     RLWPLDVRYAHRHGSSGHGRDHVLPAFVAPSIMLPVVDGAPALGTWQSVVLVDSNVDN
                     PKRTVRLSFVPA"
     gene            554..>2614
                     /gene="dnaE"
                     /locus_tag="VII50_10860"
     CDS             554..>2614
                     /gene="dnaE"
                     /locus_tag="VII50_10860"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HEY5051392.1"
                     /translation="MAASDDSFVHLHVHTEYSMLDGAARLTDLFKETVAMGMPALAMT
                     DHGNVFGAYDFYKQAKAAGVTPIIGMEGYLAPGSRFERKRAALSGASIDGNAGEMYTH
                     MTLLAENTAGMHNLFKLSSLASLEGYYYKPRMDRELLQTYANGVIATTGCPSGEVNRL
                     LQQGQYELAKQAASDYRDIFGAENFYCELMDHGIDIERRHRDDLMRLARELHLPFVAT
                     NDLHYTYAKDWEAHEVLLCVQTGKTMADPNRLRMGEGQLYLKTPQEMRELWREIPEAC
                     DNTLAIAERIHVEFNEGANLMPNFPVPAGESEESWLVKEVELGLHKRFPLGVPDAHRR
                     QAEYELGVIGQMGFPGYFLVVADLVRYAQSSGIRVGPGRGSAVGSMVSYALGITQLDP
                     IHHKLLFERFLNPERISMPDIDMDFDERRRGDMIRYATEKYGEERVAQIITYGTIKAK
                     QAVKDSARVLGFPFATSDQITKVMPPPVMGKDVPLAGIFDPDHPRYGEAGEFRALYES
                     NPDVKKVVDTARDLEGLKRQWGVHAAGVILSREPLLDVIPIHRRDQDGAIITQFDMGA
                     CEALGLLKMDFLGLRNLTILDDCLAGIKNNRGDEVVLETLDLDADQNTYDLLGRGDTL
                     GIFQLEGGGMRSLLRSMRPTSFADISAVGSLYRPGPMGANAHNDYADRKNGRKPVVAI
                     HPELA"
BASE COUNT          489 a          838 c          828 g          459 t
ORIGIN      
        1 gacacggtgg ccgccttttg ccgtggcctc ggagacgggt tgctaaatgt gttcgtcccg
       61 cacgccaccg cggggatcgc actgatcgag atcgggtccg gcagcgagca cgatctcggc
      121 gccacgatcg accggctctg gcccctcgac gtccggtatg cccatcggca tggctcgtcg
      181 ggacacggcc gtgatcacgt attgcccgcg ttcgtcgcgc cgtcgatcat gctgcccgtg
      241 gttgatggtg cgccggcgct cggcacctgg cagagcgtcg tcctggtcga cagcaatgtc
      301 gacaatccga aacgcacggt gcggctgagc ttcgttcccg cgtgatttcg gggacgggtc
      361 tgttggccct cgtgtccttg tcaaaactgt cagacccttg gcgttagagt caggcggtcg
      421 ggctccggac agggctcggg caaatgttcc agcagtggca acggcaaggc cgccttcccc
      481 cggtcgacgt cccgagcacc accgcaccac ggcgtccaaa gactgggcgc ggacgacggg
      541 aggtcgcgca gctgtggcag cgagcgacga ctccttcgtc cacttgcacg tgcacactga
      601 gtactcgatg ctcgacggcg ccgcccggct gaccgacctc ttcaaagaga ccgtcgcgat
      661 gggcatgccg gcgctcgcga tgaccgacca cggcaacgtc ttcggcgcct atgacttcta
      721 caagcaggcc aaggcagcag gcgtcacgcc gatcatcggc atggagggct acctcgcgcc
      781 tggcagtcgc ttcgaacgca agcgagcggc gttgagcggc gcatcgatcg acggcaatgc
      841 cggcgagatg tatacgcata tgacgctgct cgccgagaac accgcgggca tgcacaacct
      901 gttcaagttg agcagcctgg ccagtctcga gggctactac tacaagccgc gcatggatcg
      961 cgaactgctg cagacctacg cgaacggcgt catcgcgacc accgggtgcc ctagcggtga
     1021 ggtgaaccgg ctactccagc agggccagta cgagctggcg aagcaggccg cttcggacta
     1081 tcgcgacatc ttcggagcgg agaactttta ctgcgagctc atggatcatg gcatcgacat
     1141 cgaacgccgt catcgcgacg acctgatgag actggcccgc gagttgcacc tgccgttcgt
     1201 tgccaccaac gacttgcact acacgtacgc aaaagactgg gaagcgcacg aagttctgtt
     1261 gtgcgtgcag accggcaaga cgatggccga cccgaacaga ctccgcatgg gcgaaggcca
     1321 gttgtatctg aagaccccgc aggaaatgcg cgagctgtgg cgcgagattc ccgaggcgtg
     1381 tgacaacacg ctcgcaattg ccgagcggat tcacgtcgag ttcaacgagg gcgccaacct
     1441 catgcccaac ttccccgttc cggcggggga aagtgaagag tcctggctgg tgaaggaagt
     1501 cgagctgggg ctgcacaagc gcttcccact aggcgtgccc gatgcgcatc ggcggcaagc
     1561 cgaatacgaa ctcggcgtga tcggccagat gggttttccc ggttacttcc tcgtcgtcgc
     1621 cgatctcgtg cgctacgcgc agagcagcgg tattcgggtc gggcctgggc gggggagtgc
     1681 ggtcggatcg atggtctcgt acgcgctggg tatcacgcag cttgatccga tccaccacaa
     1741 gttgttgttc gagcggttcc tcaaccccga gcgcatctcg atgcccgaca tcgacatgga
     1801 cttcgacgaa cgccgtcgtg gcgacatgat tcgctacgcg acggagaagt acggcgaaga
     1861 gcgcgtcgcg cagatcatca cctacggcac gatcaaggcc aagcaggctg tgaaggactc
     1921 cgcgcgggtg ctcgggttcc cattcgccac gtccgaccag atcaccaagg tcatgccgcc
     1981 gccggtgatg ggcaaggacg ttccgctggc tggcatcttc gatcccgacc acccgcgcta
     2041 cggcgaagcg ggcgagttcc gggcgctgta cgaatcgaac ccggatgtga aaaaggtcgt
     2101 cgacaccgcg cgcgacctcg aaggtctcaa acgtcaatgg ggtgtgcatg cggccggggt
     2161 catcttgtcc cgcgagccgc tgctcgacgt cattccgatc caccggcggg atcaggacgg
     2221 cgcgatcatc acccagttcg atatgggtgc gtgcgaggca ctcgggctgc tgaagatgga
     2281 cttcctcggc ctgcgcaacc tcaccatcct cgatgactgt ctcgccggca tcaagaacaa
     2341 ccgcggtgac gaggtcgtgc tcgagacgct cgatctcgat gctgaccaga acacctacga
     2401 cctgctcggc cgcggcgaca cgctcgggat cttccagctc gagggtggcg gcatgcgctc
     2461 gctcttgcga tcgatgcgtc cgacgtcctt tgccgacatc tctgctgttg gctcgctcta
     2521 ccgccccggg cctatgggcg ccaacgcgca caatgactac gcggatcgca agaacggtcg
     2581 caagcccgtt gtcgcgatcc acccagagct cgcc
//
LOCUS       DATCBJ010000540         3855 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_417001, whole genome shotgun sequence.
ACCESSION   DATCBJ010000540 DATCBJ010000000
VERSION     DATCBJ010000540.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 3855)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3855)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3855
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_417001"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..890)
                     /locus_tag="VII50_10865"
     CDS             complement(<1..890)
                     /locus_tag="VII50_10865"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEY5051393.1"
                     /translation="MSAEAVEVKRVPSTGAAAAKDIRTAFSSKWASIFIIAFTVVWTV
                     PTVGLLVTSLRPGKYVANSGWWTVIEHPHFTLSNYNTVLFGKGSTTAIFPYVMNSIAI
                     TIPATLITLAIATMAAYGLAWVKFKGSDTVFFIIFALQVVPLQMSLIPLLRFYNFGAH
                     IGKVPIFPALHLGGKYYPVWVSHTMFGLPLAVFLLHNFISQLPEDMMEAARVDGANHF
                     RIFRQIVLPLSTPAIAAFAIFQFLWVWNDLLVALTFAGGRPNVLPLTGRLEQLVGQYG
                     ANWQLLTAGAFIALIIPLIV"
     gene            complement(887..1900)
                     /locus_tag="VII50_10870"
     CDS             complement(887..1900)
                     /locus_tag="VII50_10870"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEY5051394.1"
                     /translation="MSASHALVTASTLSDSVGKLGQVVSAVVVFFAALLLVFFLAGRA
                     TGRFQRPLTIAIFVGPAIVLLIIGLVVPSFRTFYLSLHGQALAHAPYVGFRNYRFAFT
                     DGATQSVLLNTLLWIIVAPLAATGLGLTIAIVVDRMKHQSMVKSLIFMPMAISFVGAS
                     IIWKYVYDFKESSQPQDGLLSQVVILLGWKHPPNWLLTHPLNNFLLMVILVWIEVGFA
                     MVVLSAAIKAVPDEIIEAARVDGAHGFGLFSKIQIPMIRATLVVVLTTIMIATLKIFD
                     IVQTTTGGNFGTNVLANDMYQNAFVTDNIGLGAALALILFVGVLPLVGYNVLQLKRER
                     ANR"
     gene            complement(2032..3507)
                     /locus_tag="VII50_10875"
     CDS             complement(2032..3507)
                     /locus_tag="VII50_10875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY5051395.1"
                     /translation="MSFSLPTGRRRRLLVVVAPTVAAALLLAACSSSKKAATAPSSSA
                     APAATTSAAPPASASAAPVSAATGSGAAPAPQATTCADYAQYTSAAAKGKTVTMFGSI
                     LPPESDSLNKSWAQFESCTGIKISYEGSNDFESQLPVRVQGGTAPDLAIIPQPGLLQQ
                     MVALGAVKAPPAGVVANEDKYWNAAWKSYGSVNGTFYAAPQSANMKSLVWYSPSAMSK
                     AGYTIPTTWDQLAALSDKIAATGTKPWCGGIGSGTATGWPATDWLEETVLSTDGGQVY
                     DDWVSHKVTFEDPKIEAAMQLVANWMQNPKWVNGGYGDVKTIATTTFQDAGKPILTGQ
                     CWFLQQASFYEAQWPTGTTVSPTGQVWAFYLPPRSTSVPTPVEGGGEFTAAFSSDAAT
                     QAVQTYLSSPQWADSRIKVAPGWVSANSGVDQSLYSDGIDKLSAQYLTNAAATFRFDA
                     SDLMPSAVGSGAEWTQMTAWFGTGASIASVAKAIDAAWPAK"
BASE COUNT          785 a         1196 c         1195 g          679 t
ORIGIN      
        1 aacacgatga gcgggatgat gagcgcgatg aacgctcccg cggtcagcaa ctgccaattg
       61 gcgccgtatt gcccgaccag ctgttccaac cgaccagtca gcggcagtac gttgggtctg
      121 ccacctgcga acgtgagcgc aaccagcagg tcgttccaca cccacaagaa ctggaaaatc
      181 gcgaacgctg cgatcgccgg cgtcgacagc ggcaacacga tctggcggaa gatgcggaag
      241 tggttcgcac cgtcgacgcg ggcagcttcc atcatgtctt cgggcagctg gctgatgaag
      301 ttgtgcagca aaaaaaccgc gagcggcaac ccgaacatgg tgtgcgagac ccagaccggg
      361 tagtacttcc cgccaagatg cagggccggg aagatcggca cctttccaat atgcgcgcca
      421 aaattgtaga agcgcagcag cgggatcaac gacatctgca gcggcacgac ctgcaaggcg
      481 aaaatgatga agaagacggt gtcgctaccc ttgaacttca cccaggcgag accatacgcg
      541 gccatggtcg cgatcgcgag cgtgatgagg gtcgccggaa tcgtgatggc gatcgagttc
      601 atcacgtagg ggaagatcgc ggttgtcgag cccttcccga acaacaccgt gttgtagttc
      661 gacagcgtga agtgcgggtg ttcgatgacg gtccaccagc ccgaattggc cacatacttg
      721 cccggccgca gcgatgtcac cagcagacca accgtaggca ctgtccacac cacggtaaac
      781 gcaatgatga agatcgacgc ccacttgcta ctgaacgccg tccgaatgtc tttcgcagca
      841 gcagcaccgg tgctcggaac gcgctttacc tcgacagctt cggcgctcat cggttggccc
      901 gctctctctt cagctgcagc acgttatatc cgaccaaagg cagcacgccc acgaacagga
      961 tcagggccaa cgcggcacca aggccgatgt tgtcggtgac gaaggcgttt tgatacatgt
     1021 cgttagccaa gacattggtg ccgaagttgc ctccggtcgt cgtctggacg atgtcgaaaa
     1081 tcttcagcgt ggcaatcatg atcgtcgtca agaccacgac caacgtcgct ctgatcatcg
     1141 ggatctgaat cttgctgaac aacccgaagc cgtgcgcgcc gtcgacccgg gcggcctcga
     1201 tgatctcgtc aggaaccgcc ttgatagcgg ccgacaggac gaccatcgcg aagccgacct
     1261 cgatccagac aaggatgacc atcagcagga agttattcag tggatgggta agcagccagt
     1321 tgggcggatg tttccagcca agcaggatca cgacttggct cagcagcccg tcctggggtt
     1381 gcgacgactc cttgaagtca tagacgtact tccagatgat gctggccccg acgaacgaga
     1441 tcgccatcgg catgaagatc agcgacttaa ccatcgactg atgcttcatg cgatcaacca
     1501 cgatcgcgat cgtcaatccg agtccggtcg cggcaagtgg cgcaacaatg atccacagca
     1561 aggtgttgag caacacgctt tgcgttgccc cgtccgtaaa cgcaaatcga tagttcctga
     1621 agccgacgta tggagcgtgg gccaacgctt ggccgtgcag gctcaggtag aaagttcgaa
     1681 acgacgggac taccaggcca atgatcagga ggacgatggc cggcccgacg aagattgcaa
     1741 tcgtcagcgg ccgctggaac cgaccggtcg cgcgccctgc cagaaagaac acaagtagga
     1801 gcgccgcgaa gaatacgacg acggccgaga cgacctggcc gagtttgcct acagagtccg
     1861 aaagggtcga tgccgtgacc aacgcatgtg atgccgacac gctcactccc atcgatatgc
     1921 cgggcagtgg tgacacacgt gatgcaggta ctcggagaaa gccgtggccc ggaggttact
     1981 ccttccgggc cacggcgtcc ccgtctcagc gggacattct ctctagcggc gttacttagc
     2041 tggccacgcg gcgtcgatgg ccttcgccac gctggcgatc gacgcgccgg tgccgaacca
     2101 tgccgtcatc tgcgtccact cagcgcccga accaaccgcg gacggcatca agtcagatgc
     2161 gtcgaagcgg aaggtcgcgg ccgcgttcgt gaggtactgc gcggacaact tgtcgatgcc
     2221 gtcgctgtac aggctctggt cgactccgct gttcgcggaa acccagcctg gtgcaacctt
     2281 gatccggctg tcggcccact gcggtgacga caggtacgtc tgcacagcct gggtcgccgc
     2341 gtcggaggag aacgcagcgg tgaactcgcc gccaccttcg accggggtcg ggacgctcgt
     2401 gctacgcggc ggcaggtaga aggcccacac ctgaccggtc ggggagaccg tggtgcctgt
     2461 gggccactgt gcctcgtaga acgatgcctg ctgcaagaac cagcactgac cagtcaggat
     2521 cggcttcccg gcgtcctgga acgtcgtggt cgcgatcgtc ttcacgtcgc cgtatccgcc
     2581 gttgacccac ttcgggttct gcatccagtt ggcgaccaac tgcattgctg cttcgatctt
     2641 cgggtcctcg aaggtgacct tgtggctcac ccagtcgtcg tagacctggc cgccgtctgt
     2701 gctgagcacg gtctcctcga gccagtcggt ggccggccag ccggtcgccg taccggatcc
     2761 gatgccgccg caccacggct tggtgccggt ggccgcgatc ttgtcagaca gcgctgcgag
     2821 ttggtcccac gtcgtcggga tcgtgtagcc cgccttggac atcgccgacg gcgagtacca
     2881 gaccagcgac ttcatgttcg cgctctgggg tgctgcgtag aacgtgccgt tgacggagcc
     2941 atacgacttc caggccgcgt tccagtactt gtcctcgttg gcgacaacac cagccggcgg
     3001 agccttgacg gcaccgagcg cgaccatctg ctgcaacaac ccgggctgcg ggatgatcgc
     3061 caggtcaggt gcagtgccac cctgaacacg caccggcagc tgcgactcaa agtcgttgga
     3121 gccttcgtag gagatcttga tgccggtgca gctctcgaac tgcgcccatg acttgttgag
     3181 cgagtcggat tccggcggca ggatcgagcc gaacatcgtc accgtcttac ccttggcggc
     3241 ggcacttgtg tactgcgcgt agtcggcgca ggtcgtggcc tgaggcgcag gcgcggcacc
     3301 gctgccggtg gctgcggaga ccggggctgc ggaggccgag gcgggcggag ccgcggacgt
     3361 ggtggctgcg ggcgcggcgc tgctggatgg cgcggtggca gccttcttgc tgctcgaaca
     3421 ggcagcgagc agcagtgctg ccgcgaccgt cggagcgaca acgaccagaa ggcgacggcg
     3481 cctgccggtt ggcaagctga aactcatggt ttctcctctt gcgacagggg ccccacggga
     3541 gaaacccgcg tggctgcaaa cggtgacatc gtggccctcc tggccgacga gatcaaggcg
     3601 tgacgggcac aaaaagcgat gtatacggtt acggtttggc atcgttccca cgatcaagcg
     3661 ccaacgccct ctagtcgagt cattcgcgca gcccttaccg aaccgttgcc aacctatcca
     3721 gcaacgatcg ccacatctac gccgaatcat ctcgaaatgg caacatttcg caacgtcggc
     3781 cgatccgacc gctaggcgac tgttcggtgc agcgtcgtcg agccccgcac gcacagctgc
     3841 gtcggcagcc gctgc
//
LOCUS       DATCBJ010000541         3834 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_198763, whole genome shotgun sequence.
ACCESSION   DATCBJ010000541 DATCBJ010000000
VERSION     DATCBJ010000541.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 3834)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3834)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3834
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_198763"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..312)
                     /locus_tag="VII50_10880"
     CDS             complement(<1..312)
                     /locus_tag="VII50_10880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5051396.1"
                     /translation="MTAPGVAAAASIKATTCNAIDASKAMNDLPASDDCATGVAANGA
                     RSSAAVSPKTTPVSPTTTISSTARSFDRTNGDLLAITKVSGAATGEIAFGGNFTAVVT
                     PD"
     gene            complement(565..1338)
                     /locus_tag="VII50_10885"
     CDS             complement(565..1338)
                     /locus_tag="VII50_10885"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY5051397.1"
                     /translation="MAYGTDPSQVADLHLPTQINADGAPWPVVVVIHGGYWGSGYGRS
                     LGTGLAADLAQQGVAAWNIEYRRVGNGGGWPSTLIDVARAIDGLADIADPAADGRLDL
                     DRVVTVGHSAGGQLAMWGAGRHKLQVDAPGAQPAVRVRGVVAQAGVLDLVRGADAGLG
                     GGAVVRFLGGTPAEVPERYALASPYELLPIGVPSTLVHGMKDKVVPIDQSDRYAAAAI
                     ATGDTVDEHRLKGVDHFAPIDPRMRAWAVCRTAALSYLT"
     gene            complement(1384..1743)
                     /locus_tag="VII50_10890"
     CDS             complement(1384..1743)
                     /locus_tag="VII50_10890"
                     /inference="COORDINATES: protein motif:HMM:NF021193.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PGPGW domain-containing protein"
                     /protein_id="HEY5051398.1"
                     /translation="MAVNTVPPQVPGQRRSVTDRGQTFGDRWLPEVMMIARLNNTPAW
                     LRRPVVTLVGVVLIAVGIVLLVLPGPGLLAMAVGLAVLATEYPAAQRLLDRINGSVPV
                     AAARARVAKFRTRSQRS"
     gene            1849..2769
                     /locus_tag="VII50_10895"
     CDS             1849..2769
                     /locus_tag="VII50_10895"
                     /inference="COORDINATES: protein motif:HMM:NF015587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HEY5051399.1"
                     /translation="MKGFRSTHPPAGGQAAPNWRAVAARVRVQIVDRNLALLAAGTAF
                     WAILSIFPAIVALVTIYGIVASPAQVTKEVTRLGGSLSPSTRQVITNWLAGLTSTSHQ
                     QLGFGLVVGLLAVLWAVSTGVRTLIKAITAAYEQEETRGYLKLRGLALLASVGVIVVA
                     VVALAAIGIAPAIRHLVHNTLWRLAFDVGEWVVLALILTAAVAALYWLAPPKTSVNWR
                     WASAGAVFSTVFVVLASIGFSLYVRFFAHYNKTYGTLGGIVILMLWLYYCVFIVLLGA
                     LIDVEVERELTGNVSAATEVPATEASSSRP"
     gene            complement(2750..3439)
                     /locus_tag="VII50_10900"
     CDS             complement(2750..3439)
                     /locus_tag="VII50_10900"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012387.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0016018 - cyclosporin A binding [Evidence
                     IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEY5051400.1"
                     /translation="MHHRSLHSRLTPALIVATTMICAGACASAHSRAAATPTTVGGTI
                     SCAYSPTGQPAARSVGKPPTRASQLGTSTATLTTNQGAIVIKLDPAQAPCTVNSFVYL
                     AEKKYFDATPCHRLTTAGIFVLQCGDPTGTGSGGPGYEFADENLNGAVYPAGTVAMAN
                     AGPGTNGSQFFLVYADTTLAPNYTPFATITGGLAVIQKIAAAGSDNSNGQGDGKPNLP
                     VEITNVTVATS"
     gene            complement(3468..>3834)
                     /locus_tag="VII50_10905"
     CDS             complement(3468..>3834)
                     /locus_tag="VII50_10905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5051401.1"
                     /translation="RVWVGSIVYTVIFALVGFALAGLFRNIPAAIVTVILLPLLVENI
                     LRGLLGLHVFRSIRGLAKALPFSSGAQIFRYQPVGTNGNTGAGFEALPSPVDGLLVFL
                     AFLAIVLGLSWVLFEKRDA"
BASE COUNT          671 a         1253 c         1248 g          662 t
ORIGIN      
        1 gtcaggggtt acgaccgcgg tgaagttccc gccgaacgca atttcgccgg ttgccgctcc
       61 gctgactttc gtaatggcca ggaggtcgcc gttggtgcgg tcaaagctcc gcgcagtact
      121 gctgatcgtc gtggtcggcg agaccggcgt ggtcttcggg ctaacggccg cggacgagcg
      181 ggcgccgttc gccgcgaccc cggtcgcgca gtcgtcggag gccggaagat cgttcatcgc
      241 cttcgaggcg tcgatcgcgt tgcaagtcgt cgctttgatg gaggctgccg cggccacccc
      301 aggcgccgtc atgagcaatc caccgatact gagaccggca atagcagaaa atgccttacg
      361 catggtgtcc ccccccgggg cgaaacttcc cgtgacgcgg tcccgaatga ggactgcgta
      421 tccgggtgca tacttcccga aaagttcgtt gcaggcaatg accgacgcgt ggggagagcg
      481 acgcagggag gcgtaccccg gtgctacgtg acataatgta acttatcggc acatgcttca
      541 gacgggacgc cgagggcagc cgagctacgt caggtaactc aacgcggcgg tgcggcacac
      601 tgcccaggcg cgcatccgcg gatcgatcgg cgcgaaatgg tcgacgccct taagcctgtg
      661 ctcgtcgacc gtgtcacctg tagcgatcgc cgccgcggcg taacgatcac tctggtcgat
      721 cggcaccacc ttgtccttca tcccgtggac caaggtcgac gggacgccga tcggtagcag
      781 ctcgtacggc gacgccagcg cgtaccgctc tggaacctcg gcgggcgtgc cgcccaagaa
      841 cctgacgact gccccgccgc cgagcccggc atccgcgcca cgcacgaggt cgagcacgcc
      901 cgcctgggcc accacaccgc gaacacggac cgctggctgc gcgcccggcg cgtcgacttg
      961 gagtttgtgt cggccggcac cccacatcgc gagctgcccg ccagccgaat gtccgaccgt
     1021 gacgacgcgg tcgaggtcca gccgcccgtc cgcagccgga tcggcaatgt cagcgagccc
     1081 gtcaatggcc cgcgcaacgt caatcaacgt cgatggccaa ccgccgccgt tgcccaccct
     1141 gcggtactcg atgttccagg ccgcaacgcc ctgttgagcg agatcggccg ctaggccagt
     1201 accgagcgac cggccataac cggatcccca gtacccaccg tgaatcacca cgacaacagg
     1261 ccacggcgcc ccgtcagcgt tgatctgcgt cggcaggtgc agatccgcga cctgactggg
     1321 gtcggtccca tacgccaatg tgagcggcct aggccgccgc gctccaagtc gcacgatggc
     1381 gactcagctg cgctgactac gcgtgcggaa cttcgccacc cgggcacggg ccgctgcaac
     1441 gggcaccgaa ccgttgatgc gatcgagtaa ccgctgcgct gccggatact cagtggccag
     1501 caccgcgaga ccgacggcca tcgcgagcag gcccggcccc ggaagcacga gaagcacgat
     1561 cccgaccgcg atcaggacca ccccgaccag ggtgaccaca ggacggcgca gccatgcggg
     1621 cgtgttgttc aaacgagcaa tcatcattac ctccggcaac catcggtcgc cgaaggtctg
     1681 accgcgatcc gttacagacc gccgctggcc gggtacctgt ggaggcaccg tgttgacggc
     1741 cacagacgcg agggctactc cccttcggtg ccctcatggt acggcacgcg ccatgatgga
     1801 cggcccatga tgtccggcct gcgtgatgga cggcgaggag cgaatcgcgt gaagggtttc
     1861 cgtagcaccc acccgccggc gggcggtcaa gctgccccga actggcgtgc cgttgcggct
     1921 cgggttcggg ttcagatcgt cgaccgcaac ctcgcgctgc tcgcggccgg aactgcgttt
     1981 tgggccatcc tgtcgatctt cccggcaatc gtcgctctgg tcacgatcta cggcatcgtg
     2041 gctagccctg cgcaagtgac gaaggaagtc acccggctcg gcggctcgtt gtcaccgtcc
     2101 acgcgtcagg tcatcacgaa ttggctcgcc ggactgacca gcacgagtca tcagcagctc
     2161 gggttcgggt tagtcgtcgg acttctcgcc gtcttgtggg ctgtgtcgac cggtgtccgg
     2221 accttgatca aggcgatcac cgccgcgtac gagcaagaag agacccgcgg ctacctcaag
     2281 ctgcgcggtc tcgcactgct ggccagtgtt ggagtcatcg tggtcgccgt cgtcgcgctg
     2341 gccgcgatcg gaatcgcgcc cgcgatacga cacctcgtac acaacacgct gtggcgcctc
     2401 gcgtttgacg tcggcgagtg ggtcgtgctc gcactgatcc tcacggcggc cgtggctgcg
     2461 ctgtattggt tggcgccgcc gaagacgtcc gtcaactggc gctgggcctc ggccggcgct
     2521 gtgttctcca cggtgttcgt ggtcttggcg agcatcgggt tctcgctcta cgtgcgcttc
     2581 ttcgcgcact acaacaagac ctacggcact ctcggcggca tcgtcatctt gatgttgtgg
     2641 ctctactact gcgttttcat cgtgttactc ggggccttga tcgacgtcga ggtcgagcgc
     2701 gaactgactg gcaacgtgag tgcagcgact gaggttccag cgacagaggc tagctcgtcg
     2761 cgaccgtgac gttggtgatc tcgacgggca ggttcggctt gccgtcaccc tggccattgc
     2821 tgttgtcgga accggcggcc gcgatcttct ggatcacggc caggccgccg gtgatcgtgg
     2881 cgaacggtgt gtagttcgga gcgagggtgg tgtcggcgta gaccagaaag aactggctgc
     2941 cgttcgtgcc cgggccggca ttggccatcg cgaccgtgcc ggcgggatag acggccccgt
     3001 tgaggttttc gtcggcgaac tcatagccgg gaccaccgct gcccgtgccg gtcggatctc
     3061 cgcattgcag cacgaagatg ccggctgtgg tgaggcgatg gcacggtgtc gcgtcgaagt
     3121 acttcttttc cgcgagatag acgaacgagt tcaccgtgca cggcgcctgc gcggggtcca
     3181 gcttgataac gatcgcacct tggtttgtcg tcagcgtcgc ggtagacgta ccaagttgtg
     3241 atgcgcgcgt cggtggcttg ccgacggatc gcgcggccgg ctggccggtc ggcgaatacg
     3301 cgcagctgat cgtgccccca acggttgtcg gcgtcgcggc agcgcgactg tgcgcgctgg
     3361 cgcacgcgcc tgcgcaaatc atcgtcgtcg cgactatgag cgcaggcgtg agccgcgagt
     3421 gcaggcttcg gtggtgcacc aacaactcct ttagacaggc acgcgagcta ggcgtcgcgc
     3481 ttctcgaaca agacccacga cagaccaagc acgatcgcca ggaaggcgag gaaaaccagc
     3541 aggccgtcga ccgggctcgg cagtgcctca aaaccggcac cggtgttgcc gttggttccg
     3601 accggctgat agcgaaatat ctgcgcaccc gatgagaagg gcagtgcctt tgctaacccg
     3661 cgaatggaac ggaacacgtg caacccgagt agtccgcgaa ggatgttctc taccagcagc
     3721 ggtaacaaga tcaccgtgac gatcgctgcg gggatgttgc ggaacagccc ggcgagcgcg
     3781 aacccaacca acgcgaagat aacggtgtag acgatcgagc cgacccacac tcgg
//
LOCUS       DATCBJ010000542         2424 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_124688, whole genome shotgun sequence.
ACCESSION   DATCBJ010000542 DATCBJ010000000
VERSION     DATCBJ010000542.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2424)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2424)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2424
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_124688"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..427
                     /locus_tag="VII50_10910"
     CDS             <1..427
                     /locus_tag="VII50_10910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HEY5051402.1"
                     /translation="RVIRVKQPQRVITQSPVRNFTRIFASHPDHLAAGEAALCAVYPD
                     ARNPFAHRELLEVEGLEPWSVSEVWMSAALASGQEMRVVDVTDTADRKLAALRCHRSQ
                     YSDWNALEERVRGWLEATAKANGLEPGRLAEQFVVVPTV"
     gene            508..2361
                     /locus_tag="VII50_10915"
     CDS             508..2361
                     /locus_tag="VII50_10915"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HEY5051403.1"
                     /translation="MRLADLQLSDGDAYWIEGRPAEHGRCVIVREHDGVITDLIDAPY
                     SARTWVHEYGGAAMYAHQGTVYFSNAADGRIHTVVPGAAPEPLTPVDAGLRYADFEID
                     TARERIICVVEEHRGDSVVNDLRAVPLRGGDAVTLASGNDFYSTPRVSPDGNSLLWLT
                     WNQPNMPWDGCELWVSRLDSAGMPSGARLVAGGPKESIFQPSWSPDSVVHFTSDRTEW
                     WNLYRWNGTKVDALAPMQAECGAPQWVFGLSTYAFCGDGRIVLWACRDGGWECFVLEA
                     AGQPRRVEVPETVFALYVGADGDDLLYLAGGPETPDGVVRANVASGAHRVVRSESPDM
                     TVEASMLSRPRHVTYPGHGGETAHAWYYAPRNGSVEREAHRKPPLLLHAHGGPTSSTN
                     IALNVAVQYWTSRGFAYLDVDYGGSTGYGRTYRDRLNEQWGVVDVGDCIAGAEYLVQQ
                     GEVDPDRLFIDGGSAGGYVVLCAMTFHDVFSAGASLFGIADLEALFATPGHKFEARYD
                     SPLPKGRGMYDRSPVHFIDRVRGAVLLLQGLDDPVVPAVQAEMMFAALKRAGVPCAYI
                     GYPGEQHGFREAANIARTLEAQLYFFATVTGMSMSEAIDPVAILNLQEPGE"
BASE COUNT          396 a          776 c          840 g          412 t
ORIGIN      
        1 cagggtgatc cgcgtcaagc agccccagcg ggtgatcacg cagtcaccgg tgcgcaactt
       61 cacacgcatc ttcgccagcc atcccgacca tctcgccgcc ggggaggccg ccctgtgcgc
      121 ggtgtatccc gatgcccgca acccgtttgc gcaccgcgag ctgctcgagg tcgaaggcct
      181 ggaaccgtgg tctgtcagcg aggtctggat gagcgccgcg ctcgcgtcgg gccaggagat
      241 gagggtggtc gacgtcaccg acaccgcgga tcgcaagctc gccgcactgc gctgtcaccg
      301 cagccagtac agcgactgga acgcgctcga ggagcgggtg cgggggtggc tcgaggccac
      361 cgcgaaggcg aacggccttg agccgggacg cctcgcggag cagttcgtcg tcgtcccgac
      421 ggtatgaccc tgcgcatcag agagaacgtc acgccgtcgg ccgtctggcc ctccccgatc
      481 acagcgcagc gcgccgcggc ggggcgaatg cgacttgccg atcttcagct cagcgacggg
      541 gatgcctact ggatcgaggg tcgtcctgcc gagcatggcc gctgcgtcat cgtccgcgag
      601 cacgatgggg tgatcaccga tctgatcgac gctccgtact cggcgcgaac gtgggtgcac
      661 gagtacggag gcgcggcgat gtatgcgcac cagggaactg tctatttctc caatgccgcc
      721 gacggccgga tccacacagt cgttccgggg gcagcgccgg aaccgctcac gccggtcgac
      781 gccggcctgc gctatgcgga tttcgagatc gacacggcgc gtgagcggat catctgcgtg
      841 gtcgaggagc atcgcggcga ttcggtcgtc aacgacctcc gcgccgtccc gcttcgaggc
      901 ggcgacgccg tcacgctggc ttcgggcaac gacttctatt ccaccccgcg ggtgagtccg
      961 gatggcaaca gcctgctgtg gctgacctgg aaccagccga acatgccgtg ggacggatgc
     1021 gagctctggg tttcgcgtct ggacagcgcg ggaatgccat cgggcgcgcg gttggtcgcc
     1081 ggcgggccga aggagtccat cttccagccg tcgtggtctc cggactccgt cgtgcacttc
     1141 acgtcagacc gcaccgagtg gtggaacctg tatcgctgga acggcaccaa ggtggatgcg
     1201 ctcgcgccca tgcaggcgga gtgcggggcg ccgcaatggg tgttcggcct ttccacgtat
     1261 gcgttctgcg gcgacgggcg aatcgtgctc tgggcctgtc gggatggcgg atgggaatgc
     1321 ttcgtgctcg aggcggcggg tcagccccga cgcgtcgagg ttcccgagac ggtgttcgcg
     1381 ctgtacgtcg gcgccgatgg cgatgacctt ctctacctgg cgggcggacc ggagacgccg
     1441 gatggtgtcg ttcgcgcgaa cgttgcgagc ggcgcgcacc gggtggtgcg gtcggagagc
     1501 cctgacatga ccgtcgaagc gtcgatgctc tcgcggccgc gtcacgtcac gtaccccgga
     1561 catggtggag agacggcgca cgcctggtac tacgcaccac gcaatggctc cgtggaacgg
     1621 gaagcacacc gcaagccacc gctgctgctc cacgcgcacg gcgggccgac gtcgtccacg
     1681 aacatcgcgc tcaatgtcgc ggtgcagtac tggaccagcc gcggcttcgc ctacctcgac
     1741 gtcgactacg gcggcagcac cggctacggc cgcacgtacc gcgatcgtct caatgagcag
     1801 tggggcgttg tggacgtcgg cgattgcatc gcgggtgccg aatacctggt gcagcaaggc
     1861 gaggtcgatc ccgaccgtct gttcatcgac ggcggcagcg ccggcggata tgtggtgctg
     1921 tgcgcgatga cgttccacga tgtcttctct gcaggtgcaa gcctcttcgg catcgcggac
     1981 ctggaagccc ttttcgcaac gcccggacac aaattcgagg cgcgctacga ctcgccattg
     2041 ccgaaaggca ggggaatgta cgaccgctcg ccggtgcact tcatcgatcg cgtgcgcgga
     2101 gccgtgctgc tgctgcaggg acttgacgac ccggtggttc cggccgtgca ggcggagatg
     2161 atgttcgctg cgctcaaacg tgccggagtg ccgtgcgcgt acatcggata cccgggcgag
     2221 cagcacggct tccgtgaggc cgccaatatc gcgcgaacgc tcgaagctca gctgtatttc
     2281 ttcgccaccg tgaccggcat gtccatgagc gaagcgatcg atccggtcgc gatcctcaat
     2341 ctccaggagc cgggcgaata acggtcaggt ggctttggtc caagatcgga ggtgtcgggc
     2401 gtgcgaaaga tcgtcgtata cgag
//
LOCUS       DATCBJ010000543         3704 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_198833, whole genome shotgun sequence.
ACCESSION   DATCBJ010000543 DATCBJ010000000
VERSION     DATCBJ010000543.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 3704)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3704)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3704
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_198833"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..376)
                     /locus_tag="VII50_10920"
     CDS             complement(<1..376)
                     /locus_tag="VII50_10920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5051404.1"
                     /translation="MAPGHPRGTAAQWRSLLPPDRPPALFRWAKYLLLTGVVVFAAST
                     VLVPTDSSARPFLDGWLYDSMTVLGAIVIGGRAWCVATGRLAWSLLAVGIAANAVGDV
                     VYTLFVGSNGPFPSIADPFYLAF"
     gene            complement(412..1575)
                     /locus_tag="VII50_10925"
     CDS             complement(412..1575)
                     /locus_tag="VII50_10925"
                     /inference="COORDINATES: protein motif:HMM:NF014135.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HEY5051405.1"
                     /translation="MPRPKRPPAWLIAAAAGAICVASRALPWHEAVATTKRVGPILLF
                     LVAITVVAELADAAQVFDVAATRAAKLGRGRTWGLFALIVVLGSLTTIVLSLDTTAVL
                     LTPVVLALASQLRISVIPFAMAAVWLANTASLLLPVSNLTNLLAVNRLHLSAVSFAAK
                     MWLPALASIAVTAVVIGVAYRRDLRGQYPVPGTFDIADRISFRISATVCVVIAPFFVI
                     GLPVWLPASVAAVVLVALFLFRQPHRVRTSLLPWQLVVLVQGLFLVVATLGAHGLDRL
                     LGDAAGHSDGAIGVLRTAGVGAGASNLANNLPSYLALERVTDGRQHQLLGLLLGTNVG
                     PLVLLWGSLATLLWRERCKARGVEISAWQFARLGLTGVPILLIATWGALLVTK"
     gene            1624..3060
                     /locus_tag="VII50_10930"
     CDS             1624..3060
                     /locus_tag="VII50_10930"
                     /inference="COORDINATES: protein motif:HMM:NF012975.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNB domain-containing ribonuclease"
                     /protein_id="HEY5051406.1"
                     /translation="MPHRRVRLSVDGAALREGFAAIRTELHVPAAFPPEVLAAAAQAA
                     ARWTPATSTAPDQTDVPYLTIDPPGSMDLDQAMHIVRGGTGYRVRYAIADAAAFVSPG
                     DPVDVEAHVRGETLYSPDMRTPLHPPAIGEAAASLLPDQVRPALVWTIDLDADGRQTA
                     VDVKRALVRSTSRLNYSQAQQMIDAKSGAGAADGTLALLAEVGRLRQALEVERGAVSL
                     RLPEQEVDADRSGFRLHYRAPLPVEDWNAQISLLTGMAAAGIMLGANVGILRTLPPPD
                     VGAIGLLHRASHALGVPWPKHQTYQAFVRALDPASDVGAALIELSTRLLRGAGYTAFD
                     SPPAQPNHSAVAAPYAHATAPLRRLIDRYVGELCVAVCAHLDVPPWIRQALPALADVM
                     ADSGHKAHALERACVDLVETVLMASHVGETFEGGVVETMKHGGIVQLRTPPVRARCEA
                     EGLLLGARIDVRLTTADPATRTLLFVPA"
     gene            3087..3508
                     /gene="rnpB"
                     /locus_tag="VII50_10935"
     ncRNA           3087..3508
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="VII50_10935"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
BASE COUNT          646 a         1213 c         1286 g          559 t
ORIGIN      
        1 aaaaggccag gtagaacggg tcggcgatcg acgggaacgg cccgttgctg ccgacgaaga
       61 gcgtgtagac gacgtcgccc acagcgttgg ccgcgatccc gacagcaagc agactccacg
      121 cgaggcgccc ggtggcgacg caccatgccc gccccccgat cacgatcgct ccgagcaccg
      181 tcattgagtc gtacagccag ccatcgagga aaggcctcgc gctcgagtcg gtcggcacga
      241 gcacggtcga cgcggcgaac accaccacgc cggtcagcag caggtatttg gcccagcgaa
      301 acaacgccgg cggacgatca gggggcagca gggatcgcca ctgtgccgcc gtgccgcgcg
      361 ggtgtccagg cgccatgcct ccgtatcgac cggcgcccgt ccgatctgac cctatttcgt
      421 gacgagcaac gcgccccagg tggcgatcag caagattggc actccggtga gaccgagccg
      481 ggcgaactgc cacgcgctga tctcgacgcc gcgcgccttg catctttccc gccagagcag
      541 cgttgcgagc gatccccaga gcaggacaag cgggccgacg ttcgtgccga gaagcagtcc
      601 gagcagctga tgttggcgtc cgtcggtgac ccgctcgagc gcgaggtagc tcggcaggtt
      661 gttcgcaagg ttgctcgcac ctgcaccgac accggctgtc cgcaagacac cgatcgcgcc
      721 gtcgctgtgg ccggccgcgt cgccgagcag ccggtcgagg ccgtgggcgc ccaatgtcgc
      781 gacgacgagg aacagtcctt gaaccaggac gaccaactgc catggcagca gcgaggtccg
      841 tacgcgatgt ggctgacgaa agagaaagag cgcgaccagg acgacggcgg ccacgctcgc
      901 cggcagccac accggtagcc cgatgacgaa gaacggcgcg atgacaacac agactgtcgc
      961 gctgatgcga aagctgatcc ggtcggcgat gtcgaacgtg ccgggcaccg ggtactggcc
     1021 ccgcagatct cggcgatacg cgacgccgat gaccacggcg gttacggcga tagacgccag
     1081 ggccggcagc cacatctttg cggcgaagga caccgccgac agatgcagcc ggttgacggc
     1141 gagcaggttg gtcaggttcg acaccggcag cagcaaactc gcggtgttcg ccaaccagac
     1201 ggcggccatc gcgaacggga tcaccgatat ccgcagctgc gatgcgagcg cgagtacaac
     1261 gggtgtgagc aggacggcgg ttgtgtcgag gctgagcacg atcgtggtga ggctgccgag
     1321 gacgacgatg agagcaaaga gcccccacgt tcgaccgcgg ccgagcttgg cggcgcgggt
     1381 cgccgcgacg tcgaacacct gcgccgcatc cgccagttcg gcgacgacgg tgatggcgac
     1441 aaggaacagc agaatcggcc cgactcgttt cgtggtcgcg accgcttcat gccaaggcag
     1501 tgcacgactc gccacgcaga tcgcgccggc cgcagcggcg atcagccacg ctggcggcct
     1561 tttcggtctg ggcacactca gagcctgccc cacccatgat cgtttctcac atacggtggc
     1621 gacatgccgc accgcagggt tcgcctcagt gtcgacggag ctgcgttgcg cgagggattc
     1681 gcggccatcc gtaccgaact ccacgtgccg gcggcatttc cgcccgaggt gctcgccgcg
     1741 gcggcacaag ccgcggcccg ttggaccccg gcgacgtcaa cggcgccgga ccagaccgac
     1801 gtcccatatc tcacgatcga cccgccgggc tcgatggacc tcgaccaggc gatgcatatc
     1861 gtccggggcg gcactggcta tcgggtgcgc tacgcgatcg ctgatgccgc agcgttcgtg
     1921 tcgccggggg atccagtcga cgtcgaggcg catgtgcgcg gcgagaccct ctacagcccc
     1981 gacatgcgga cgccgctaca tccgcccgcg atcggtgagg ccgcggcgag cctgctgccc
     2041 gaccaggtcc ggccggcgct cgtgtggacc atcgacctcg atgccgatgg gcggcagacg
     2101 gcggtcgatg tcaagcgggc gctcgtgcgg tcgacgtcgc ggctcaacta cagccaggcc
     2161 cagcagatga tcgacgcgaa gtccggtgca ggtgcggcgg acggcacgtt ggcgttgctc
     2221 gccgaggtag ggcggcttcg gcaagccctt gaagtcgagc gcggcgccgt cagcctgcgc
     2281 ctccccgaac aagaggtgga cgccgaccgg tcggggttcc gattgcacta ccgcgctccg
     2341 ttgccggtcg aggactggaa cgcgcagatc tcactgctca ccggaatggc agccgccggc
     2401 atcatgctcg gcgcgaacgt cgggatcctt cgcacgttgc cgccgcccga cgtgggcgcg
     2461 atcggattac tgcaccgagc gtctcacgcg ctgggcgtcc cgtggccgaa acaccagacg
     2521 taccaggcat ttgtgcgtgc gctcgatccg gcgagcgacg tcggcgcggc actgattgag
     2581 ctgtcgacgc ggttgctgcg cggggccggc tacacggcgt tcgacagtcc gccagcgcag
     2641 ccgaatcaca gtgcggtcgc ggctccctac gcgcacgcaa cggcgccgct gcgaaggctg
     2701 atcgatcggt acgtgggcga actgtgcgtg gcagtctgcg cgcacctcga cgtcccgcct
     2761 tggattcgtc aagctctacc tgcgcttgct gatgtcatgg ccgactccgg gcacaaggcg
     2821 catgctctcg aacgtgcgtg tgtcgacctc gtcgagacag tgctgatggc gtcgcatgtg
     2881 ggggagacgt ttgagggcgg cgtcgtcgag acgatgaagc acggtggcat cgtgcaattg
     2941 cggacgccgc cggtgcgcgc tcgatgcgag gcggaaggcc tgctgctggg cgcgcggatc
     3001 gacgtccggc taacaacggc tgacccggcg acgcgcacgc tgctgttcgt gccggcctga
     3061 ccgtctacag ttgccggtac ggcggacgag tcggccgggc gaccgcgttg catgccttcg
     3121 ggtatgtgcc gaggaaggtc cgggctccac agggcaaggt ggtgggtaac gcccacccgg
     3181 ggtgacccgc gggacagtgc cacagaaaac agaccgctac cgagttctgg ccttggccgg
     3241 agttcggcag taagggtgaa acggtggtgt aagagaccac cagcgtgcgg ggtgactcgc
     3301 gcggcttggt aaaccccacc tggagcaagg tcaagaggcg ttgttgggct tcggctcggt
     3361 ggcgctgcgc aggcgtttga gggcggcccg cccgagcctg cgggtagacc gctggaggct
     3421 gtcggcaacg gcagtcgtag atggatggtc gcccgcctgg accgcaaggt tcaggtgaca
     3481 gaacccggcc tacgcgccga ctcgtccgcc ccaccccgtt tgacctgcac gtttgcactt
     3541 gcggatagct tggcgtcaca cagatgtcac gtcggaggcg cgagcgttcg cgtcgactct
     3601 gtgcttggtc tggccctcgg attcggacaa taggcttccc ctaggaattc gtcatgtctc
     3661 ggcacttgcg tctgccgagc gccggacaga acgaggcgac gcga
//
LOCUS       DATCBJ010000544         2982 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_456453, whole genome shotgun sequence.
ACCESSION   DATCBJ010000544 DATCBJ010000000
VERSION     DATCBJ010000544.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2982)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2982)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2982
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_456453"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(80..1099)
                     /locus_tag="VII50_10940"
     CDS             complement(80..1099)
                     /locus_tag="VII50_10940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013228421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03842 family LLM class F420-dependent
                     oxidoreductase"
                     /protein_id="HEY5051407.1"
                     /translation="MEFGVVLQCNPPAWRTVELAKQAELAGFGYVWTFDSHLLWQEPY
                     VIYSQILSATHRIIVGPMVTNPATRDWTVTASTFATLNEMFGNRTVCGIGRGDSAVRV
                     INGKPVSLAELRDAIAVIRRLANGDAAEYKDSTLRLTWNPHSRLDIYVAAYGPKALAL
                     TGEVGDGFILQLADLAVAEWSIKAVRDAAAAAGRDPASVKICVAAPAYVGTDIAHQRE
                     QCRWFGGMVGNHVADIVARYGADGGPVPQALTDYIAGRQGYDYNEHGRVGNTHADFVP
                     DEIVDRFCVLGTVDEHIEKLRALKALGVDQFAVYLQHDAKEATLQAYADKVIPALADT
                     VAAKS"
     gene            complement(1129..2544)
                     /gene="hydA"
                     /locus_tag="VII50_10945"
     CDS             complement(1129..2544)
                     /gene="hydA"
                     /locus_tag="VII50_10945"
                     /EC_number="3.5.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR02033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004157 - dihydropyrimidinase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropyrimidinase"
                     /protein_id="HEY5051408.1"
                     /translation="MTILVKNGTVVSPTGAIAADVLIDGERIVALLEPGTTRLGVDLE
                     ATADVVIDARDKYVVPGGVDSHTHMQMPFGGTQASDTFETGTRAAAWGGTTTIIDFAI
                     QRTGERVLDGLAAWHEKAGGNCAIDYGFHQIIGGVDDESLKAMDELVSEGISSIKLFM
                     AYPGVFYSDDGQIVRAMQTAAANGSMVMMHAENGIAIDVLVAQAIARGDTAPRYHGLT
                     RPWETEEEATHRAIMLARMTGAPLYIVHMSAKQAVATLAAARDDGWNVFGETCPQYLY
                     LSLEEHLSAPDFEGAKYVCSTPLRSRAEGHQHELWKALRTNDLSVVSTDHCPFCMKEQ
                     KELGIGNFAKIPNGIGGVEHRMDLIYQGVVDGHITLARWVELCSTTPARMFGLYPRKG
                     VIAPGADADVVVYDPNATTRISVDTHHMNMDYSAWEGFEVAGRVDTVISRGTVVVRDR
                     EFVGTKGHGRYLKRGQSQYLL"
     gene            complement(2564..>2982)
                     /locus_tag="VII50_10950"
     CDS             complement(2564..>2982)
                     /locus_tag="VII50_10950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016332446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HEY5051409.1"
                     /translation="FDTAIGKIGVYICYDRHFPEGWRALGLGGAQIVFNPSATSRGLS
                     SYLWKLEQPAAAVANEYFIGAINRVGVEELGDDDFYGTTYFVDPEGKFVGDVGDAYSP
                     DLVVRDLDLDLLTEVRNRWAFYRDRRPEAYGDLTRP"
BASE COUNT          509 a          979 c          945 g          549 t
ORIGIN      
        1 gcactcatcg gttcgcgcgt gcacacccgg cccaatccgg acgccgcgct caccgacctg
       61 acctgaccgc gccgccacct caggacttgg cggccaccgt gtcggccagc gccggaatca
      121 ccttgtctgc ataagcctgc agggtggcct ctttggcgtc gtgttgcagg tatacggcga
      181 actggtcgac gcccaacgcc ttcagcgccc gcaacttctc gatgtgctca tcgacagttc
      241 ccagcacaca gaatcgatcg acaatctcgt ccggcacgaa gtcggcatgg gtgtttccga
      301 cccggccgtg ctcgttgtag tcgtagcctt gccgccccgc gatgtagtcg gtcaacgcct
      361 gcggcaccgg cccgccgtcc gcgccgtatc gagcaacgat gtcggcgacg tgattgccga
      421 ccatgccacc gaaccatcgg cattgctcgc gctggtgggc gatgtcggtg ccaacgtaag
      481 cgggtgctgc cacgcagatc ttcacggatg ccggatcgcg accggccgca gccgccgcgt
      541 cgcgtactgc cttgatgctc cattccgcaa ccgcgaggtc ggcgagctgc aggatgaatc
      601 cgtcgccaac ttcgccggtc agtgcgagcg ccttcggccc ataggccgct acgtagatgt
      661 ctaggcggct gtgcggattc caggtcagtc gcaacgtgct gtccttgtat tcagcggcgt
      721 caccattggc gagcctgcga atcacggcga tcgcgtcgcg cagctcagca agtgaaaccg
      781 gcttgccgtt gatcacccgc acggcagagt cgccgcggcc gatcccgcac actgtgcgat
      841 tgccgaacat ctcgttgaga gtcgcgaatg tcgatgcggt gacggtccag tcgcgagtgg
      901 ccggattggt gaccatcggg cccacgatga tgcgatgtgt cgccgacagg atctggctgt
      961 agatcacata cggctcctgc cacagcaggt gcgagtcgaa ggtccacacg taaccgaagc
     1021 cggcaagctc ggcctgcttc gccaactcga ccgtgcgcca tgccggtggg ttgcattgca
     1081 gcacgacgcc gaactccatc ggtgcccctt ctccgttgcg gatgctggtt agagcaggta
     1141 ctgcgattga ccgcgcttga ggtaacggcc gtgacccttc gtgccgacaa actcgcgatc
     1201 gcggacgacg acggtgcctc tcgagatgac cgtgtcgacc cgacccgcga cctcaaagcc
     1261 ctcccacgcc gagtagtcca tgttcatgtg atgggtgtcg accgagatgc gggtcgtcgc
     1321 gttcgggtcg tacaccacga catcggcatc ggcgcccggc gcgatgacgc ccttgcgggg
     1381 gtacaggccg aacatccgcg ccggggtcgt cgagcacagc tcgacccaac gagcgagggt
     1441 gatgtgacca tcgaccacgc cctggtagat gaggtccatc cggtgctcga cgccgccgat
     1501 gccgttggga atcttcgcga agttgccgat gccaagttcc ttctgttctt tcatgcagaa
     1561 cgggcagtgg tcggtgctga cgaccgacag atcgttggtg cgcagcgcct tccacaactc
     1621 gtgctggtga ccttcggcgc gcgaccgcag cggtgtcgag cacacgtact tcgcgccctc
     1681 gaagtctggc gccgacaagt gttcttcgag cgagagatag aggtactgag gacacgtctc
     1741 gccaaagacg ttccagccgt cgtcgcgagc tgcggcgagc gtcgccaccg cttgcttggc
     1801 cgacatgtgc acgatgtaga gcggcgcgcc ggtcatgcgc gccagcatga tcgcccggtg
     1861 ggtggcctcc tcctcggtct cccatggcct cgtgaggccg tggtagcgcg gcgcggtgtc
     1921 gcctcgtgcg atggcctgcg caacgaggac gtcgatggcg atgccgttct ctgcatgcat
     1981 catgaccatc gagccgttcg ctgcggccgt ctgcatcgcg cgcacgatct gcccgtcgtc
     2041 ggaatagaac acgccgggat aagccatgaa cagcttgatg ctgctgatgc cttcactgac
     2101 cagctcgtcc atagccttca acgactcgtc gtcgacgccg ccaatgatct ggtggaagcc
     2161 gtagtcgatc gcgcagttac cgccagcctt ctcgtgccac gcggcgaggc cgtcaagcac
     2221 ccgctcgccg gtgcgctgaa tcgcgaaatc gatgatcgtc gtcgtgccgc cccacgcggc
     2281 agcacgagtg ccggtctcga atgtgtccga tgcctgcgtg ccgccgaacg gcatctgcat
     2341 gtgcgtgtgg gagtcgacac cgcccggcac gacgtacttg tcgcgggcgt cgatgacgac
     2401 atcggccgtc gcttcgaggt cgacgccaag tctcgtcgtc cccggttcga gaagtgcgac
     2461 gatccgctcg ccgtcgatta ggacgtcagc cgcaatcgcg ccggtgggac tcacgaccgt
     2521 gccgttcttc accaagatcg tcatggggtg ctccggttcg cgtctagggc cgggtgaggt
     2581 cgccgtacgc ctcgggacgc cgatcgcggt agaacgccca gcgattgcgc acctcggtca
     2641 gcaggtccag gtcgagatca cgcacgacga ggtcaggtga gtaggcgtcg ccgacgtcgc
     2701 cgacgaactt gccctcgggg tcgacaaagt aggtcgtgcc gtagaaatcg tcgtcgccga
     2761 gctcctcgac accgactcgg ttgatggccc cgatgaagta ttcgttcgcg acggcggcgg
     2821 ccggctgctc gagcttccac aagtacgacg aaaggccgcg cgaggtggcc gacggattga
     2881 acacgatctg cgcaccgccc agacctagcg cccgccagcc ttcgggaaag tgccggtcgt
     2941 agcagatgta gacgccgatc ttgccaatcg cggtgtcgaa ga
//
LOCUS       DATCBJ010000545         6156 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_43318, whole genome shotgun sequence.
ACCESSION   DATCBJ010000545 DATCBJ010000000
VERSION     DATCBJ010000545.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 6156)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6156)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6156
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_43318"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1601
                     /locus_tag="VII50_10955"
     CDS             <1..1601
                     /locus_tag="VII50_10955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018036474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HEY5051410.1"
                     /translation="ERTRDHTGAMAGQTESGLPIEPVYDSTALADFDPATALGEPGAF
                     PFTRGVYPTMYTGRLWTMRQYAGFGSAAESNRRYRQLLAAGTTGLSVAFDLPTQMGYD
                     SDDPLAAGEVGKVGVAIDSIDDMRTLFDEIPLDQVSTSMTINAPAALLLLMYQMVAEE
                     NGIAASALRGTVQNDILKEYVARGTYIFPPRASLRFATDLFAYCKTELPAWNTISVSG
                     YHMAEAGATPVQEVAFTLAHAIEYVRAAQAAGLGVDEFAPRLTFFFVARTTFLEEVAK
                     FRAARRIWAHVMRDEFGARDPSSMMLRFHTQTAGVQLTAQQPELNMVRVALQALGAVL
                     GGTQSLHTNAYDEAIALPTEKAARLALRTQQVIAYETDVAKTADPFAGSYVIESLTDD
                     IETAVRELMRRVEDLGGAVAGIERGFQRAEIENAAYEVAQGIDSGDRIVVGLNAFTSE
                     ADEDYQPLRMDPASEAQQIARLAAIRARRDGDVVARTLAAVAVAASGDQNVLPPIKEA
                     LRAQATLGEVCATLRGIWGRYEPS"
     gene            1710..1958
                     /locus_tag="VII50_10960"
     CDS             1710..1958
                     /locus_tag="VII50_10960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5051411.1"
                     /translation="MTPIIVAVAVAVVIVVGVFLVMMTRSRRDDEGERFRRVADLTSR
                     WSRQVQAPSGESGTPSRAAARPEMAQPGAGKTESVDPS"
     gene            complement(2193..2438)
                     /locus_tag="VII50_10965"
     CDS             complement(2193..2438)
                     /locus_tag="VII50_10965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5051412.1"
                     /translation="MSRTMHALIAPPAAGAELTGAAGLDTAGAELAAAEVDAAEVAGA
                     AALVVAGAGALLAAGLALFLLEQPARMIATVPVAAII"
     gene            2425..3387
                     /locus_tag="VII50_10970"
     CDS             2425..3387
                     /locus_tag="VII50_10970"
                     /inference="COORDINATES: protein motif:HMM:NF014647.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HEY5051413.1"
                     /translation="MVLDTGGVDDHSFNQSSWQGLQDANKANPKIAISYVSSNTQNDY
                     TPNLNAEVAKGCSTIIAVGGLMADAVKTAAAANPKQQFAEIDNPSTGPNVYGMQFNTA
                     QGGFLGGYLAASVSKTGVVATFGGLNIPPVTVYMDGFWEGVQYFNKVKGKSVKVLGWN
                     ETNQKGGTFDPSTSNPFGDTAGGQAISKAFIAQGADVIFPVAGGTGEGAAIAAKASGG
                     KVSVIWVDTDGCVSDAADCSVFLSSVVKGLASAVTTYANDFAAGKSLGGGSYIGTLAN
                     NGTGLAPFHDFASKVPASVQSELTQITADIESGKITITSPSQPK"
     gene            3544..5187
                     /locus_tag="VII50_10975"
     CDS             3544..5187
                     /locus_tag="VII50_10975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016289947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY5051414.1"
                     /translation="MEGIRVAGRTGVAVKLELRGITKRFGSLVANDHIDLVVEPGEIH
                     CLLGENGAGKSTLMNVLYGLYAPDEGEILVDGVVTRFNGPGDAMAAGIGMVHQHFMLI
                     PVFSVAENIMLGHEDTRGPGAFGWLDRRAARRKVADVSKRFGLAVPPDALVADLPVGV
                     QQRAEILKALARDAKVLVLDEPTAVLTPSEIDDLITVMRRLRDAGTSIVFISHKLKEV
                     MAVGDTITVIRRGRVVGQPAVSSSEQELASLMVGRPVLLTVEKATAQPGAVVLDVEGL
                     RVVDAAGVVQVDDVSFSVREGEIYAIAGVQGNGQTEVTECLVGLENPTAGRIRVLGND
                     VTTSSVRERLDLGIGYVPEDRSHDGLVGSFSVEENLVLDVHESAAFAGRFGLDMAAIG
                     ANGLARVDEFDVRCEDPRAAASTLSGGNQQKVVLARELSRPLKLLVVAQPTRGLDVGS
                     MEFVHKRIVRERDERTAVVLISTELDEVLGLADRIAVMYQGRIIGELPGGSSAAAVGL
                     LMAGSVAGAEDAPETIGAAIFEPIEVVEPGEGSEGGAAP"
     gene            5277..>6156
                     /locus_tag="VII50_10980"
     CDS             5277..>6156
                     /locus_tag="VII50_10980"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY5051415.1"
                     /translation="MIALSFVTAIVVGGILIAVTSQPTRNAARHFSASPGKVFSDAWH
                     AVSAAYVALFEGAIFDPHTFGHGSIWHDFNPLSETLYNATPLILVGLSVGLAFRTGLF
                     NIGGQGQLILGAMAAGYVGFTWHLPPVIHLVVAILAGVIAGAAWGGIAGFLKARSGAH
                     EVITTIMMNYVALDLLDYALKTSAFRRPGSNQAISPIVHGTARLGKLLPSPLRVNLGL
                     LIAIVATVGIAWLLTRSRLGFTLRAVGASPTAARTAGMNVGRTYVTAMLIAGGLAGLA
                     GCSQILGGGTNYALTNA"
BASE COUNT         1049 a         1933 c         2087 g         1087 t
ORIGIN      
        1 ccgaacgcac ccgggaccat actggggcga tggcgggtca gacggaatcc gggctgccga
       61 tcgagcctgt gtacgacagc acggcgttgg ccgatttcga cccggctacc gcgttggggg
      121 agccaggcgc ttttcctttt acgcgtggcg tctatccgac catgtacacc ggccggctgt
      181 ggaccatgcg gcagtacgcg ggcttcggat cggcggccga atcgaaccgc cgctaccgcc
      241 aactactagc agcggggacg acgggactgt ctgtcgcgtt cgacctgccc actcagatgg
      301 gctacgactc cgatgacccc ctcgccgcag gcgaggtcgg caaggtcggc gtggcgatcg
      361 actcgatcga cgacatgcgc acgctcttcg acgaaatccc gcttgaccag gtatcgacgt
      421 ccatgacgat caacgcgccg gctgccttgc tgttgctgat gtatcagatg gtcgccgagg
      481 aaaacggcat cgcggcctcg gctctgcgcg gcaccgtgca aaacgacatc ctcaaggagt
      541 acgtggcccg cggcacctac attttccccc cacgtgcgtc actgcggttt gcgaccgatc
      601 tctttgccta ctgcaagacc gagttgccgg cctggaacac gatctcggtc tccgggtacc
      661 acatggccga ggcgggcgcg acgcccgtcc aagaagtcgc gttcaccctg gcgcacgcga
      721 tcgagtacgt cagggctgct caggcagcag ggctcggggt cgacgaattc gctcctcggc
      781 ttacgttttt ctttgtcgca cgcacgacgt ttctcgaaga agtcgccaag ttccgagccg
      841 cgcgtcgaat ctgggcgcac gtcatgcgcg acgagttcgg tgcgcgcgat ccgagttcga
      901 tgatgctgcg cttccataca cagaccgccg gcgtccagct cacggcccag cagccggaac
      961 tcaacatggt gcgcgtcgct ttgcaagcgt tgggcgcagt gcttgggggc acgcagtcgc
     1021 tgcataccaa tgcgtacgac gaagcgatcg cattgccgac ggagaaggcg gcccggttgg
     1081 cgttgcgaac ccagcaggtg atcgcatacg aaactgatgt cgcgaagacc gccgatccgt
     1141 ttgccggttc ttacgtcatc gagtcgctca cagacgacat cgagacggcc gtgcgcgaac
     1201 tcatgcgccg tgtcgaagac ctcggtggcg cggtcgccgg catcgaacgc ggattccagc
     1261 gcgccgagat cgagaacgcc gcgtacgagg tcgcgcaggg catcgacagc ggtgaccgga
     1321 tcgtggtcgg cctcaatgcc tttacatcgg aggcggacga ggattaccag ccgttacgta
     1381 tggatccggc gtcggaagca cagcagatcg cccggctcgc ggcgatccgc gctcgccgtg
     1441 acggcgacgt cgtcgcgcgc acgctggctg cggtcgcggt ggccgcgagt ggcgaccaga
     1501 acgtgttgcc gccgatcaag gaggcactga gagcgcaagc caccctcggc gaggtatgtg
     1561 ccacgttgcg aggtatctgg ggtcgctatg agcccagttg aggatcttgt gttctcccca
     1621 cggccccttg taatccgtgg ccgagttgga cgtggcggcg tcgtgcggtg agataggggt
     1681 acgtccccgc cggacgaaag gagcctgatg tgacaccgat catcgtggcg gtcgctgtgg
     1741 cggtggtcat cgtcgtcggc gtgttcttgg tgatgatgac ccgctctcgt cgcgacgacg
     1801 agggagaacg tttccgtagg gtcgccgatc tgacctcgcg gtggagccgg caggtccagg
     1861 cgccatccgg ggaatcgggt acgccgtcca gggcggcagc caggccagaa atggcgcaac
     1921 ctggagcggg caagacggaa tcagttgacc ccagctgact cctgagactg ggcgcgcagt
     1981 tccttcgatg gctcggcccg agtgaacgtg agatcacggc gacgtaacgt tcttggcgcg
     2041 cttcgatagc aaacaggctg ccacgcgctc agcgcggcgg gtgatcgtgg cgctgagctt
     2101 ggaccggtcg cgcgcgcgcc gcctgaccca gctattgtcc acccaacatt ttacttgcga
     2161 tgcgatagcg gaggcgttgt gaagaagacc tatcagatta ttgcggccac aggaaccgta
     2221 gcgatcatcc ttgcgggctg ttcgagcaag aagagcgcca gccctgctgc gagcagcgca
     2281 ccggctcccg ccaccacaag tgccgcagcc ccggccacct cggcagcatc gacctcggcg
     2341 gcggccagct cggcaccggc agtgtccagc ccggcggccc cggtcagctc ggcgccagct
     2401 gcgggcggtg cgatcaacgc gtgcatggtt cttgacaccg gcggtgtcga tgaccactcg
     2461 ttcaaccagt cgtcgtggca gggactgcag gatgccaaca aggcgaaccc gaagattgcc
     2521 atctcctacg tctcgtcgaa cacccagaac gactacacgc ccaacctgaa cgccgaggtt
     2581 gctaagggtt gctcgacgat catcgcggtc ggcggcctga tggcggacgc cgtaaagacg
     2641 gccgccgcgg cgaacccgaa gcagcagttt gctgagatcg acaacccttc gaccggcccg
     2701 aacgtgtacg gcatgcagtt caacaccgcg caaggcggct tcctcggtgg atacctcgcg
     2761 gcatccgtct cgaagaccgg cgtggtcgcg accttcggtg gcttgaacat cccgccggtg
     2821 accgtctaca tggacggctt ctgggagggc gtgcagtact tcaacaaagt caagggtaag
     2881 agcgtgaagg tgctcggctg gaacgagacg aaccagaagg gcggcacgtt cgacccgagc
     2941 acgtcgaacc cgttcggtga caccgcgggt ggccaggcga tcagcaaggc gttcatcgca
     3001 caaggcgccg acgtcatctt cccggttgcc ggtggtacgg gtgagggagc agcgattgcc
     3061 gcgaaggcgt cgggtggcaa ggtcagcgtg atctgggtcg acaccgatgg ctgcgtcagc
     3121 gacgctgctg actgctcggt gttcttgagc agtgtggtca agggcctggc cagcgcggtc
     3181 accacgtacg cgaacgactt cgcggcaggc aagtcgctcg gcggcggaag ctacatcggc
     3241 accttggcga acaacgggac cggcttggcg ccgttccacg acttcgcgtc gaaggttccg
     3301 gcgagcgtgc agtccgagct cacccagatc acggccgaca tcgaaagcgg caagatcacg
     3361 atcacgtcgc cttcgcagcc gaagtaaggg cagtagagct ggtaagcacg cagtagacgt
     3421 aggcgcaggt gggcgggtcc cgtgacgggc ccgcccacct cttgcccgtt ccatgcgcta
     3481 cgactgccca ttccatgggg gtaggccttt ggtgttgact gcggtggtgc tgactgccgc
     3541 tgcttggagg gcatacgtgt cgccggccga actggggtcg cggtgaagct cgaactgcgc
     3601 ggcatcacga agcggttcgg ctccctcgtc gcgaacgatc acatcgacct ggttgtcgaa
     3661 ccgggcgaga tccactgctt gctgggcgaa aacggcgcag gcaagagcac cctgatgaac
     3721 gtcttgtacg ggctgtacgc ccccgacgag ggtgagattc tcgtcgacgg cgtcgtcacc
     3781 aggttcaacg gcccgggcga tgcgatggcc gctggcatcg gcatggtcca ccaacacttc
     3841 atgctgatcc cggtcttctc ggtcgccgag aacatcatgc tcggccacga agacacgcgc
     3901 ggtcccggtg ccttcggctg gctcgaccgg cgcgccgcga ggcgcaaggt cgccgatgtc
     3961 tcgaagcggt tcggtctcgc cgtcccgcct gatgctctgg tcgccgatct gccggtcggc
     4021 gtgcagcaac gcgccgagat cctcaaggcg ctcgcccgcg acgccaaggt gctggtgctg
     4081 gacgaaccaa ccgccgtgct cacgccgtcc gagatcgatg acctcatcac ggtcatgcgc
     4141 cggttgcgtg acgcgggtac ctcgatcgtt ttcatcagcc acaagctgaa ggaggtcatg
     4201 gcggtcggtg acacgatcac cgtgatcagg cgcggtcggg tcgtcggtca gccggcggtg
     4261 tcgagcagcg agcaggagct cgcgtcgctg atggtcgggc ggcccgttct gttgaccgtg
     4321 gagaaggcga ccgcgcaacc cggtgcagtt gtgctggacg tcgagggctt gcgggtcgtc
     4381 gacgccgcgg gggtggtgca ggtcgatgac gtgtcgttct ccgtgcgcga gggcgagatc
     4441 tacgccatcg ccggcgtgca ggggaacggg cagaccgagg tcaccgagtg cctcgtcggc
     4501 ctcgaaaatc cgacggccgg ccgcatccgt gtgcttggca acgacgtcac cacgtcgtcc
     4561 gtgcgcgagc ggctcgacct cgggatcggc tacgtgccgg aggatcgctc acacgacgga
     4621 ttggtaggca gcttctccgt cgaggagaac ctcgtgctcg acgtgcacga gagtgctgcc
     4681 tttgccgggc ggttcggtct cgacatggcg gcgatcggtg ccaacggtct cgccagggtt
     4741 gacgagttcg acgttcgctg cgaagacccc cgggcggcgg cgagcacgct gtcgggcggg
     4801 aaccagcaaa aggtcgtact cgcccgtgaa ctgtcgcgtc cactgaaact gctggtggtt
     4861 gcccagccga cgcgcggtct tgatgtcggg tcgatggagt tcgtgcacaa gcgcatcgtc
     4921 cgggagcgcg atgagcggac cgcggtggtg ctcatctcga ccgagctcga tgaggttctg
     4981 gggttggccg accggatcgc cgtcatgtac caaggccgca tcatcggcga gctgccaggc
     5041 ggtagctcgg cggctgcggt cgggctcttg atggcgggct ctgtcgcggg cgccgaagac
     5101 gctccggaga cgataggtgc ggccatcttc gaacccatcg aggtagttga accgggcgaa
     5161 ggttcagagg ggggtgcagc cccctgagcg tcgacggcac agtcaaggac gagatcgccg
     5221 ttccgcccgg tgggccggcg cgacgcaagt cccggagcga ggcgcatccg ctggtcgtga
     5281 tcgccttgtc gttcgtcacc gcgatcgtgg tcggcggcat cctcattgcg gtcaccagtc
     5341 agccgacccg caatgccgcg aggcacttct ctgcgtcgcc cggcaaggtg ttttccgatg
     5401 cgtggcacgc ggtgtcggcg gcgtacgttg cgctgttcga gggcgcgatc ttcgaccccc
     5461 acacgttcgg acacggcagc atctggcacg acttcaatcc gctgtcggaa accttgtaca
     5521 acgcgacgcc gctcattctc gtcgggctat ccgtcggcct cgcgtttcgc accgggctgt
     5581 tcaacatcgg cggccagggt cagctcattc tcggcgcgat ggctgccgga tacgtgggct
     5641 tcacctggca cttacccccg gtgatccacc tggtcgtggc gatccttgcg ggcgttatcg
     5701 cgggtgcagc atggggcgga atcgccgggt ttctcaaagc gcgcagcggc gcgcacgaag
     5761 tcatcaccac aatcatgatg aactacgtcg cgctcgatct gctcgactac gccttgaaga
     5821 ccagtgcgtt tcggcggccc ggcagcaacc aagcgatctc gcccatcgtg cacggaacgg
     5881 cccgtctagg aaagctgctg ccgtccccgt tgcgggtcaa cctgggcttg ctgatcgcga
     5941 tagtggcgac ggtgggcatc gcatggttgt tgacgcgtag tcggctcggt ttcaccttgc
     6001 gagcggtggg cgcgagcccg accgccgcgc gtacggcggg catgaacgtc ggtcgcacct
     6061 acgtcaccgc catgctgatt gccggcgggt tggcgggcct ggcgggctgc agccagattc
     6121 tcggcggcgg tacgaactac gcgctgacga acgccg
//
LOCUS       DATCBJ010000546         3184 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_493761, whole genome shotgun sequence.
ACCESSION   DATCBJ010000546 DATCBJ010000000
VERSION     DATCBJ010000546.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 3184)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3184)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3184
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_493761"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1714
                     /locus_tag="VII50_10985"
     CDS             <1..1714
                     /locus_tag="VII50_10985"
                     /inference="COORDINATES: protein motif:HMM:NF016047.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cell wall-binding repeat-containing protein"
                     /protein_id="HEY5051416.1"
                     /translation="VQLTGYAVALAVDTTTHHVYALEQFPQTEPPGGLAPKAVPASIA
                     VIDPSTLSVITTYPNLGNGPAFPSTGNTLVDDPVGHRLFNGSVNVMLNGREVDGATQI
                     DTVTGAVTALSVSTAYRPNSLVYDTATQGLYFTVEGFPQTVAVVSVSGAPRFLTSIEV
                     GPLPDGIALDPSTQMVYVANNGGPSVSVINAQTNKVVATVPAGAILSGIGVDTSSHTV
                     FAANGADGDVRVIDGATNTVTGTIQVGTPQYDVAVDPTTHSVWTTSSLGASLTALKPI
                     VARFAGANRFATAAAISAYEYGDNQADAVVLARADNFPDALVGAPLAAAKNAPLLFTS
                     GTTLPAVTEAEIPRVLARGKTVYVLGGPSAIPNQIVAQLTALGYGPIRLAGGDRFETA
                     VAVADALGDPTTIFLASGSDFADALTAGPPATQAHGAILLTSPGGVAITTNTYLQAHP
                     GTVFAIGGPAAAADPKATPIFAGDRFATGIAVAQQFFPTATHAGIASGGAFPDAMAAG
                     AYMAATGGPLLLTQPTGLTASVASYLGSTDTAVTSADIFGGSTAVSPAVQTAASAALA
                     TQGG"
     gene            complement(1711..2589)
                     /locus_tag="VII50_10990"
     CDS             complement(1711..2589)
                     /locus_tag="VII50_10990"
                     /inference="COORDINATES: protein motif:HMM:NF024091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY5051417.1"
                     /translation="MSSAEMTMSLPGPAVAPHVRYHGVFLRQFVSETSIIFRRRRNIA
                     ILAVLGAVPVFIGVAVKISAPHGGQGPAFIGQITSNGLFLAFAALTLCLPVFLPVAVA
                     VVAGDAVAGEANTGTLRYLLTVPVSRTRLLVVKTAGVIAFIIAAILLVTVIGVVLGVS
                     LFGTHGMTLLSGDTVSVSNGLLRSLGVAAYVFVDLLGLAGIALFFSTLTEVPVGAMAA
                     TVVLAIIFTVLDAVPQLGGIRGILLTHHWLDFASLLRSQVNTTLLWHGMLVPLSYFAI
                     FTAAAWSRISTADVTS"
     gene            complement(2576..>3184)
                     /locus_tag="VII50_10995"
     CDS             complement(2576..>3184)
                     /locus_tag="VII50_10995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY5051418.1"
                     /translation="TDPSTSASRIGSALERVGLSAAAKKTYRRYSLGMKQRLGLAAAL
                     LRPRDLLILDEPTNGLDPQGTREVRALIRELAADGTTVLVSSHLLAEIEQVATHVGIM
                     SAGKLLRQGQLDQVLGVAAALLRIETPDVERALGVLAQLGVAVSGQSADHLLVAMGDR
                     TPDVINAALVRADVAVRGLHVERPLLEDLFVKLTGEGFDVVR"
BASE COUNT          636 a         1063 c          984 g          501 t
ORIGIN      
        1 ggtgcaactg actggttacg ccgtcgcgtt ggccgtcgac acgacgaccc accatgtgta
       61 cgccctcgag cagtttccgc aaacagaacc cccagggggc ctggccccga aggccgtacc
      121 ggcaagcatt gccgttatcg acccgagcac gctcagcgtc atcactacgt accccaacct
      181 cgggaacgga cccgccttcc cgtccaccgg caatacgctg gtcgacgacc cggtcggtca
      241 ccgtttgttc aatggcagcg tcaacgtcat gctcaacggg cgcgaggtag atggtgcgac
      301 gcagatcgac acggtgacag gcgcggtcac cgcactgtcg gtctccactg cttataggcc
      361 taactcgttg gtatacgaca cagctaccca gggcctgtat ttcaccgtgg agggtttccc
      421 gcagacggtc gcggtcgtgt ccgtcagtgg tgcgccccgc tttctcacgt cgatcgaggt
      481 tggtccactg cccgacggca tcgccctcga tccttcgaca cagatggtgt acgtcgcgaa
      541 caatggtggc ccgtccgttt cggtcatcaa cgcccaaacc aacaaggtcg tcgcgacagt
      601 ccctgccggc gcgattctca gcggcatcgg ggtcgacaca tcgagccaca cggtgttcgc
      661 ggcaaacgga gccgacggcg acgttagggt cattgacggc gcaacgaaca cggtgacggg
      721 cacgatccag gtcggaacgc cgcaatacga tgtggcggtc gatcccacaa cccactcggt
      781 atggacaacg tcgtccctcg gcgcgtcgct gaccgcgctc aaacccatcg tcgcgcgctt
      841 cgccggtgcg aatcgctttg caacagcggc cgcgatctcg gcgtatgagt acggcgacaa
      901 tcaggcggac gccgtggtgc tggcgcgagc cgacaacttc cccgacgccc tggtcggcgc
      961 tccgttggcc gctgccaaga atgcgccgct gctgttcacc tcaggaacga cgttgccagc
     1021 cgttaccgag gccgagattc cgcgcgtgtt ggctcgcggc aaaaccgtgt atgtgctcgg
     1081 cggcccgagc gcaattccca accaaatcgt cgcgcaacta accgcgcttg ggtatggccc
     1141 gatcagactc gcaggcggag atcggttcga gactgcagtc gcggttgccg acgcacttgg
     1201 tgaccccacg acgatctttc tggcatccgg cagtgatttc gccgatgcgc tcaccgctgg
     1261 accacccgcg acgcaggccc acggcgcgat tctgctgacc agcccgggcg gcgtcgcgat
     1321 aaccactaac acctacctcc aagcgcatcc gggaaccgtc tttgcgatcg gtggacctgc
     1381 tgccgcggcg gatcccaaag cgacaccgat ctttgctggg gatcggttcg ccaccggaat
     1441 cgctgtggcg cagcagttct ttcccacggc aacacatgcc ggcatcgcga gtggtggcgc
     1501 attccccgac gcaatggcgg ccggtgcata catggcggcc actggtggcc cgctgctgct
     1561 gacccaaccg acgggcctca cggcctccgt ggccagttac ctcggatcga ccgacaccgc
     1621 cgtcacgtcc gctgacatct tcggcggtag cactgcagtg tcaccagcgg tacagaccgc
     1681 agccagcgca gccctcgcaa cccaaggagg ctaagacgtg acgtcggcgg tcgagattcg
     1741 cgaccacgcc gcggcggtga agattgcgaa gtagctcagc gggaccaaca tgccgtgcca
     1801 cagcagagtc gtgttgacct gcgaccgcag caagctggcg aagtcgagcc agtgatgcgt
     1861 aaggaggatc ccgcgaatgc cgcccaactg cgggacggcg tcgagcaccg tgaagataat
     1921 cgcgagcaca accgtggccg ccatcgcgcc gaccggaacc tccgtcagcg tcgagaagaa
     1981 caacgcgatt cccgcgagtc cgagcaggtc gacgaatacg tacgccgcga ccccaagcga
     2041 gcgcagcaac ccgttactga ccgacacggt gtcgcccgac agaagtgtca ttccgtgtgt
     2101 gccaaacagg gataccccca gcaccacgcc gatcacggtc accaaaagaa tggcagcgat
     2161 gatgaaggcg atcacgcccg cggtcttcac caccagcagc cgagtgcgtg agacagggac
     2221 cgtgagtaag tagcgcagag ttccggtatt cgcctcgccg gccaccgcat caccggcgac
     2281 gacagcaacg gctacgggca ggaacacggg caggcacaag gtgagcgcag caaatgcgag
     2341 gaacaatccg ttgctcgtga tctggccgat aaacgctggt ccctggccgc catgcggagc
     2401 gctgatcttg accgcgactc cgatgaagac cggaaccgcg ccgagcacgg caaggatcgc
     2461 gatattgcgg cggcgccgga agataatcga cgtctcgctg acgaactggc gaaggaagac
     2521 tccatgatag cgaacgtgag gagcaaccgc cggcccaggc agcgacatgg tcatctcagc
     2581 ggacgacatc gaaaccctcc ccggtcagtt tcacgaacag gtcctcaaga agcggccgct
     2641 cgacgtgtaa cccgcgcacc gcgacgtccg ctcggactag cgccgcattg atgacatcgg
     2701 gagtccgatc acccatcgcc acgagcaaat gatcggccga ttggcccgaa acggccacgc
     2761 ccagctgcgc gagcacaccg agggcgcgtt cgacatcggg cgtctcgatg cgcagcaacg
     2821 cggctgcaac gccgagcacc tgatcgagtt ggccctggcg gagcagcttg ccggcgctca
     2881 tgatgccgac gtgagtggcg acctgctcta tctcggcgag caggtgactc gagacgagca
     2941 cagtggttcc gtcggcggcg agttcgcgaa tcagcgcacg cacctcacga gtgccctgcg
     3001 ggtcgaggcc gttcgtcggc tcgtcgagaa tcagcaagtc gcgcggccgc agcaacgcgg
     3061 ccgcgagccc gaggcgctgc ttcataccga gcgagtaccg gcgatacgtc tttttcgcag
     3121 ctgccgagag tcccactcgc tccagggcag aaccgattcg ggaagcggac gtcgaggggt
     3181 cggt
//
LOCUS       DATCBJ010000547         3791 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_346409, whole genome shotgun sequence.
ACCESSION   DATCBJ010000547 DATCBJ010000000
VERSION     DATCBJ010000547.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 3791)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3791)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3791
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_346409"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..636
                     /locus_tag="VII50_11000"
     CDS             <1..636
                     /locus_tag="VII50_11000"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: protein motif:HMM:NF025793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0052717 - tRNA-specific adenosine-34
                     deaminase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HEY5051419.1"
                     /translation="LPVGALAMHEWDSAQVGVRRSLELAHQALRAGGLPVGAVILDAS
                     ANLSAEGRNHCCYDPPAGDDPFQGTPLAHAEMNALARIATSTDLSGCTLWSTHCPCTM
                     CAAACAFTGVGEVRFLAPDPSDTDSADPQNVDDRWAVLGNVLFLEGITRYSGPGAPMI
                     SRARVREPETAALLSSIGPILFDSPELEPGLVGGWPLIEAAARARADRRRG"
     gene            746..2386
                     /locus_tag="VII50_11005"
     CDS             746..2386
                     /locus_tag="VII50_11005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5051420.1"
                     /translation="MALFAVLASACTASHSPKAHRSATAASSPFPAGVSSPQAFSPPP
                     SSPPSSALPSSPAPSSAPVPPSPAASSPPAAVATGVRGTVVVGGATPARISISVSQAL
                     FSSAATVVVASPTDAAGVASGAQLAEQLDVPLLLLDHGGAAPAGGTPTPALFAAELTR
                     LGTSTVVAIGTSAADVSAAAPSVSVVGSTTQTPPAASSDGDHLTVLVPKGVNAASDAV
                     AMMASASAQAAGATVVAVQGADPRRDPTAIAALAAHPPRNVLAVGSQFGTPATVAARV
                     AVASTGTQLPGGGQIFFPGRRLVALYGHPGSGALGVLGAQDLPSSIARAKQLAATYAP
                     MSTVPVVPTFEIIATVAEAAAGSDGNYSGESSVASLLPWVQQATAAGLYVVLDLQPGS
                     ANVLSQAKLYTSLLAMPDVGLAIDPEWALGPGQHPLGQIGSLTSAQINAVGAWLEQLT
                     ASKHLPQKLLVLHQFRLSMIGDEAALKTNYNDLAVLIHMDGEGTTPSKIATWNAVVHT
                     APKGLPFGWKNFYKEDHPTMTPEQTMAQKPTPVMISYQ"
     gene            2420..>3791
                     /locus_tag="VII50_11010"
     CDS             2420..>3791
                     /locus_tag="VII50_11010"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEY5051421.1"
                     /translation="MTIGEKPVRDIEPMGDVAASLTRASGIDPVRMAIALEVFEELDE
                     LPVDHPQAIAVRRATARVYKTVKQRNRKERRAAVVAADEAVTAATATGAPGRIDDETQ
                     GLALTATTTAPTVGTLGKARACYICKERYRDVDAFYHQLCPSCAALNRSRRDARTDLT
                     GRRALLTGGRAKIGMYIALRLLRDGAHTTITTRFPGDAVRRFTDMHDSSQWLHRLSIV
                     GIDLRDPSQVVELADAVAAQGPLDILINNAAQTVRRTPGAYAQLIAAESAPLPDGPRP
                     ELIELGYSVGAAPAAIGGHPVTHALTPHALTALALTSGSASLERVAEQTAIDAGGLVP
                     DLDAVNSWTRRVHEVDPVELLEVQLCNVIAPFVLVSRLRASMAAATARRKYVVNVSAM
                     EGQFGRIYKGAGHPHTNMAKAAVNMLTRTSARDLFAEGILMTSVDTGWITDERPHPTK
                     ARLADEA"
BASE COUNT          615 a         1291 c         1272 g          613 t
ORIGIN      
        1 ttaccggttg gtgctctagc catgcatgag tgggacagtg cccaggtcgg tgtgcggcga
       61 agcctcgagc tcgcacatca agcgttgcgc gccggtggcc tacctgttgg cgcagtcatc
      121 ctcgatgcat ccgcgaacct cagcgcggag gggcgcaacc attgctgcta cgacccaccg
      181 gctggcgacg atccgtttca gggcacgcca ctagcgcacg ctgagatgaa tgcgttggcc
      241 aggattgcga cgagcaccga cttgtcgggg tgcacgttgt ggagcactca ctgcccttgc
      301 acgatgtgcg ccgccgcgtg tgcgttcacc ggcgttggtg aggtccgctt cctcgcccct
      361 gacccctccg acaccgacag cgccgacccg cagaacgtcg atgaccggtg ggcagtgctc
      421 gggaacgtct tgttccttga gggcatcacc cgctactccg ggccgggggc accgatgatc
      481 agccgggccc gagtgcgtga gccggagacg gccgcgctgc tcagcagcat cggaccgatc
      541 ttgttcgatt cgcccgagct tgagcccggg ttggtgggcg gctggccgct catcgaagcc
      601 gcggcgcgcg cccgcgccga ccgccgccgc gggtaggcgc cgcccctgcg ggaacccgcg
      661 cgccgccgac ggttgtcgca tactggggcg gatgtcttgc tcacgcgcta ccaccaggac
      721 cgacatacgg gcccggttgg cggcggtggc gctgtttgcg gtccttgcta gcgcctgcac
      781 cgcctcccac tcgccgaagg cccatcgctc ggcgaccgcg gcgtcgagcc cgttcccggc
      841 gggtgtgagc agcccgcagg cattcagccc accaccgtcc agcccaccgt cgtcggcgct
      901 gccgtcgagt cccgcgccga gctcggcacc ggtgcccccc tcgcccgccg cgtcgagccc
      961 accggccgcc gtcgccaccg gagtccgtgg aacggtcgtc gtgggtggtg cgacgccggc
     1021 caggatctcg atctcggtca gccaggcgct gttcagctcg gcggcgacgg tcgtggtcgc
     1081 gtcgccgacg gatgcggcgg gtgtcgcgag cggcgcccaa ctagccgagc aactcgacgt
     1141 cccgctcctt ctccttgatc acggcggcgc ggcacccgcg ggcggcaccc caacacccgc
     1201 attgttcgcg gcggagctga cgcgactggg gacgagcacg gtcgtcgcga ttggtaccag
     1261 cgccgccgac gtgagcgcag cggcgccgtc tgtcagcgtg gtggggagta cgacgcagac
     1321 tccgcccgca gcgtcgagtg atggcgacca cctcactgtt ctcgtgccga agggtgtcaa
     1381 cgcggcttcc gacgcggtcg cgatgatggc gtcggcgagt gcgcaggctg caggcgcgac
     1441 cgtcgtggcc gtgcaaggcg ccgatccgcg tcgtgatccg actgcgatcg cggccctcgc
     1501 ggcgcatccg ccgcgcaacg tcctcgcggt cggctcgcaa ttcggcaccc ctgcgactgt
     1561 ggccgcgcga gttgcggtcg cgagtacggg cacgcaactg ccaggcggcg gtcagatctt
     1621 ctttcctgga cgccgattgg ttgcgctgta tggacatccg ggcagcgggg cactcggcgt
     1681 cctgggcgcg caagatttgc cctcgagcat cgcccgtgcc aagcagcttg ctgcgaccta
     1741 cgcaccgatg agcactgtgc cagtcgtccc gaccttcgaa atcattgcca ccgtcgcgga
     1801 agccgctgcg ggtagcgacg gcaactattc gggggagtcg agcgtcgcat cgcttcttcc
     1861 gtgggtgcag caggcgactg ccgcggggtt gtatgtcgtt ctcgatctgc aaccgggcag
     1921 cgccaacgta ttgtcgcaag caaagctgta cacgtcgttg cttgccatgc ccgacgtagg
     1981 gctggcgatc gacccggagt gggcactagg cccgggccag cacccgcttg ggcagatcgg
     2041 cagcctcacg agcgcgcaga tcaacgcggt cggtgcgtgg ctcgagcagc tgaccgcgtc
     2101 gaagcatctg ccgcagaaat tgctggtact acaccagttc agactctcga tgattggtga
     2161 cgaggccgca ctgaagacca actacaacga cctcgcggtg ctgattcata tggacggaga
     2221 gggcaccacg ccgagcaaga tcgcgacctg gaacgctgtc gtgcacacgg cgccgaaagg
     2281 cctgccgttc ggatggaaga acttctacaa ggaagaccac ccgaccatga cacccgagca
     2341 gacgatggcg caaaagccta cgcctgtcat gatttcctat cagtagctgc acgctctccc
     2401 gacagaacgg cgacctcgaa tgacgatcgg cgagaagccg gtgcgcgaca tcgagcctat
     2461 gggcgacgtc gccgcttccc tcacgcgcgc ctcgggcatc gacccggtgc gcatggcgat
     2521 cgcgctggag gttttcgagg aactcgacga gctccctgtc gaccatccgc aggcgatcgc
     2581 cgtgcgccgc gccactgcac gtgtctataa gacggtgaaa cagcgcaacc gcaaggagcg
     2641 gcgggcagcc gtggtcgcag cggacgaagc cgtgaccgcc gcaacggcga ccggcgcgcc
     2701 aggccggatt gatgacgaga cgcaaggctt ggcgttaacg gcgacaacga ccgcccccac
     2761 ggttggcacc ctgggcaagg cgcgggcgtg ctacatctgc aaagagcgct accgcgacgt
     2821 cgacgcgttc taccaccagt tgtgtccgtc gtgtgcagcc ctgaatcgaa gccgtcgtga
     2881 cgcgcgtacg gatctgaccg gccgtcgggc attgctgacc ggaggtcgcg ccaagatcgg
     2941 catgtacatc gcgttgcgat tgctccgcga tggtgcgcac accacgatca ccactcggtt
     3001 tcccggcgat gcggtgcgcc gcttcacgga catgcacgac agttcgcagt ggttgcatcg
     3061 cctgagcatc gtcggcatcg acctgcgcga tccttctcag gtggtagaac tcgcggacgc
     3121 agttgcggca cagggaccgc tcgacattct catcaacaac gccgcgcaga ccgtgcgccg
     3181 tacgccgggc gcctacgcac agctcattgc cgcggagagc gcgccgctgc cggacggtcc
     3241 gcgtccggag ctgatcgagc tcgggtactc tgtgggcgcc gcgccggccg caatcggcgg
     3301 gcatcccgtg acccacgcgc tgacgccaca tgcgctgacc gccctggcct tgaccagcgg
     3361 gtcggcatcg ctcgagcgcg tcgccgagca gacggctatc gacgccggcg gcctggtgcc
     3421 cgatctcgat gcggtcaaca gctggactcg gcgggtacat gaggtcgatc ccgttgagtt
     3481 gctcgaagtg cagctgtgca atgtgatcgc gccgttcgtg ctggtcagcc ggctgcgcgc
     3541 atcgatggcg gccgcaacag cgcgacgcaa gtacgtcgtg aacgtgtccg cgatggaggg
     3601 ccagttcggc cgcatctata agggcgccgg ccatccgcac acgaacatgg cgaaggccgc
     3661 cgtcaacatg ctgacgcgga ccagcgcgcg cgacttgttc gccgagggca tcctcatgac
     3721 gagcgtggac accggttgga tcaccgacga gcggccgcat cccaccaagg cgcggctcgc
     3781 cgatgaggct t
//
LOCUS       DATCBJ010000548         2138 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_497786, whole genome shotgun sequence.
ACCESSION   DATCBJ010000548 DATCBJ010000000
VERSION     DATCBJ010000548.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2138)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2138)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2138
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_497786"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            9..509
                     /locus_tag="VII50_11015"
     CDS             9..509
                     /locus_tag="VII50_11015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017956046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HEY5051422.1"
                     /translation="MQLESMYQEIILDHYRTPHHKGLREPYDVEVHHVNPTCGDEITL
                     RVKLDGDTVTDVSYDSAGCSISQASASVLTDLVIGRDIETGLVAHREFVALMQSRGAA
                     NPDEEILEDAVAFTGVSKYPARVKCALLSWMAWKDATAQAREKTLEPAAGISPAPASI
                     PEGSTS"
     gene            506..817
                     /locus_tag="VII50_11020"
     CDS             506..817
                     /locus_tag="VII50_11020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HEY5051423.1"
                     /translation="MTTTVDDVKEALYDVVDPELGINVVDLGLIYDLALDDQGVATID
                     MTLTSAACPLTDVIEDQTRSALDGLVTDFKINWVWMPPWGPDKITDDGREQLRAIGFN
                     V"
     gene            866..>2138
                     /locus_tag="VII50_11025"
     CDS             866..>2138
                     /locus_tag="VII50_11025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018226048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEY5051424.1"
                     /translation="MITATDVEINVGSRVLLDAVTFRVAPGDRIGLVGRNGAGKTTLT
                     KVLAGELLPAAGSVTNTGEIGYLPQDPRTGDLDVLARDRILSARGLDIVVRDMRAAEN
                     SMGSADDGVRDAAVRKYSRIEDHFHVLGGYAAESEAASIAASLGLPDRVLGQPLGTLS
                     GGQRRRVELARILFSGAETLLLDEPTNHLDADSIAWLREFLRSHKGGLVLISHDVTLL
                     ETTVNRVLHLDATRAVLDVYNVGWKVYLEQREADERRRKRERANAERKATTLQAQADR
                     MRYKATKATAAQNMEKRAHKLLAGLEEVRKNDRVAKLRFPEPAPCGKVPLSATGLSKS
                     YGSLEVFTDVDLAIDRGSRVVILGLNGAGKTTLLRTLAGVETADTGEVLPGHGLKLGY
                     YAQEHETLDPDATVLETMRSSSPDLADVELRR"
BASE COUNT          408 a          674 c          688 g          368 t
ORIGIN      
        1 ttcggctgat gcaactcgaa tcgatgtatc aagaaatcat cctcgaccac taccgcacgc
       61 ctcaccacaa gggtctgcgg gagccttacg acgtcgaggt gcaccacgtc aacccgacat
      121 gcggcgacga aattacgttg cgagtcaagc tcgacggcga caccgtgacc gacgtgtcct
      181 acgacagtgc cgggtgctcg atcagccaag cgagcgcgtc cgttctcacc gatcttgtca
      241 tcggccgtga catcgagacc ggcctggtcg cgcatcgcga gttcgtcgcg ttgatgcaaa
      301 gcaggggggc cgcgaacccc gatgaggaga tcctcgaaga cgcggtcgcg tttaccggcg
      361 tctccaagta ccccgcgcgg gtgaaatgcg cgttgctctc gtggatggcc tggaaggacg
      421 cgaccgcaca agcgcgcgaa aagacgcttg aaccggcggc cgggatttcc ccggcgcccg
      481 cctcgattcc ggaaggcagc acctcatgac cacgaccgtt gatgacgtca aggaagccct
      541 atacgacgtg gtcgaccccg agttgggcat caacgtggtt gacctggggc tcatttacga
      601 cctggccctc gatgaccagg gcgtcgcgac catcgacatg acactgacct cggcggcttg
      661 cccgctcacc gacgtcatcg aggaccagac ccgcagtgcc ctcgacggtc tggtgacgga
      721 cttcaagatc aattgggtct ggatgccacc gtggggcccg gacaagatca ccgacgacgg
      781 ccgggagcag ttgcgcgcca tcggattcaa cgtctgagcg ttgtttcgtg gccagccgct
      841 gcacgctatc gaagggtatg ttttcgtgat caccgctacc gacgtcgaga tcaacgtcgg
      901 ctcccgggtg ttgctcgacg ccgtgacctt ccgggttgcg ccgggcgacc gcatcggtct
      961 ggtcgggcgc aacggcgcag gcaagaccac gctgaccaag gtgctcgccg gcgagttgct
     1021 tccggcggcc ggctccgtga cgaacaccgg cgagatcggc tacctcccac aggacccgcg
     1081 caccggagac ctggacgtac tcgcgcggga tcgcatcttg tcggcgcgcg gtctcgacat
     1141 cgtggtccgc gatatgcgag cggccgagaa cagcatgggc agcgctgacg atggcgtgcg
     1201 cgatgctgcg gtccgtaagt acagccgtat cgaggaccat ttccacgtcc tgggcggata
     1261 cgctgcagag tccgaagccg cgtcaatcgc ggccagcctc gggctgcctg accgtgtcct
     1321 cgggcagccg ctcggcacgt tgtctggtgg acaacggcgt cgggtcgaac ttgcccgcat
     1381 cttgttctcc ggcgcagaga cgttgctcct tgacgaaccg acgaaccacc tcgacgccga
     1441 ttccatcgcg tggctcaggg aattcctgcg gtcacacaag ggcgggctgg ttctcatcag
     1501 ccacgacgtc acgttgctgg agaccacggt gaaccgggtt ttgcatctcg acgcgacgcg
     1561 cgccgtgctc gatgtctaca acgtcggctg gaaggtctac ctcgagcagc gagaggccga
     1621 cgagcggcgc cgcaaacgcg agcgggcgaa cgcggaacgc aaagcaacga cattgcaggc
     1681 gcaggcggat cgcatgcgct ataaggcgac aaaggcaact gctgcacaaa acatggagaa
     1741 gcgagcccac aaattgctgg ctggcttgga agaagtccgc aagaacgatc gggttgcgaa
     1801 gctgcggttc cccgagccgg cgccgtgcgg caaggtgcca ttgtcagcca ccggtctgtc
     1861 gaagtcttac gggtctctcg aagtcttcac cgatgtcgac cttgcgatcg acaggggcag
     1921 ccgggtcgtg atcctcggcc tgaacggtgc gggtaagacg acgctgctgc ggacactggc
     1981 cggtgtcgag actgcggaca ccggtgaagt gcttcctggt cacggcttga agctcggcta
     2041 ctacgcccag gaacacgaga ccctcgaccc cgatgcgaca gtgctcgaga cgatgcgctc
     2101 ctcgtctccg gatttggcgg acgtcgaatt gcggcggg
//
LOCUS       DATCBJ010000549         2347 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_85343, whole genome shotgun sequence.
ACCESSION   DATCBJ010000549 DATCBJ010000000
VERSION     DATCBJ010000549.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2347)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2347)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2347
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_85343"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1796)
                     /locus_tag="VII50_11030"
     CDS             complement(<1..1796)
                     /locus_tag="VII50_11030"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY5051425.1"
                     /translation="MSASNSALLPVADIAEVKAYAATLIRKQGRAAGGVLVLYALAGA
                     AGLAGPRLLGDIVGAVQKGTTRAHVDRIAMILAGFVVAQAIFVRLARYASSVIAEKVF
                     AELREEFLRKVISLPLSTVERAGTGDLLTRMTSDIDAMSRSVRFAVPETMIAIATGVL
                     TLIAAFITAPLVALPCLIVFPLLFIATRWYLKRAPQGYLRERRVYSHMFAGITETLEG
                     GRTIDALGLRDKRIRRGDDDVRRAYVAERYTLQLRTLWYPNTETAYVVPVVAALLVGG
                     LLANSGHASLAQVTAVTLYFQQLVDPVDRLLGWLDELQVGASSLARLVGVGRVPADRV
                     ATGAKPEGERLRADGVHFAYRAGRDVLHGIDLDVRPGERIAMVGPSGAGKSTLGRLLA
                     GIHGPGAGSVTVGGVPLVDLEIDQLRREVALVTQEHHVFVGSVADNLRLAKPSADDDQ
                     LRSVLSSIDALDWVTALPEGLDTMVGSGGLILTPAQAQQIALARLVLADPHTLVLDEA
                     TSLLDPRAARRLERSLAAVVEGRTVIAIAHRLHTAHDADRVAVMVGGRIVELGSHDEL
                     IAKGGSYADLWASWHGEGPGSAGLHTSGPHISG"
     gene            complement(1793..>2347)
                     /locus_tag="VII50_11035"
     CDS             complement(1793..>2347)
                     /locus_tag="VII50_11035"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY5051426.1"
                     /translation="ERVLFGNVPLTAMALDDVRATILISDKDPRLFAGSLRDQLDPHR
                     RASAAEVDDVIDAAVAHDAVESVPGGLDGVLDERALGLSGGQRQRIALARALLVDAPV
                     LVLDEPTSAVDAYTEARIADTLHAHRAGKTTIVMSTSPLMLDRADRAVLLEGNRVVAD
                     GRHRELVSHDARYRSVVTRDEESA"
BASE COUNT          399 a          784 c          821 g          343 t
ORIGIN      
        1 ccggaaatgt gcggaccgga agtgtggagg ccagcgctgc cgggcccttc gccgtgccag
       61 gacgcccaca gatcggcata gctgccgccc ttcgcgatca gctcatcgtg gctgccaagc
      121 tcgacgatgc ggccaccgac catgaccgcc acccggtcgg cgtcgtgcgc cgtgtgcaat
      181 cggtgcgcta tcgcgatcac cgtgcggccc tcgacgaccg cggccagaga tcgctcgaga
      241 cgccgggccg cccgggggtc gagcagcgat gtcgcttcgt cgagaacaag ggtgtgcgga
      301 tcggcgagca cgagtcgagc aagcgcgatc tgctgtgcct gcgcgggcgt caaaatgagg
      361 ccgcccgacc cgaccatggt gtcgaggccc tccggcaatg ccgtgaccca gtcgagtgca
      421 tcgatcgagg agagcacaga ccgcaactgg tcgtcgtccg ccgaaggctt ggcaagccgc
      481 aggttgtcgg cgaccgagcc cacgaagacg tggtgttcct gcgtcaccaa cgcgacctcg
      541 cggcgcaact ggtcgatctc aagatcgacg aggggcacac cgcccacggt cacgctgccg
      601 gcgcccgggc cgtggatgcc ggcgagcagg cgacccagtg tgctcttgcc cgcgccgctc
      661 gggccgacca tggcgatgcg ctcgccgggt cgcacgtcga ggtcgatgcc gtgcagcaca
      721 tcgcgacccg cccggtaggc gaagtggacg ccgtccgcgc gaagtcgttc accctcgggc
      781 ttcgcgccgg tcgcgacccg gtcggccggc acccgaccga cgccgacgag gcgcgcaagt
      841 gaggatgcgc cgacctgcag ttcgtcgagc cagccgagca ggcggtcgac cgggtcgacg
      901 agctgctgga agtagagcgt gacggcggtg acctgcgcga ggctcgcgtg gcccgaattc
      961 gcgagcagcc caccgacgag gagcgccgcg acgacgggca cgacgtaggc cgtctcggtg
     1021 tttgggtacc acagcgttcg cagctgcaat gtgtagcgct ccgcgacgta agcgcgccgg
     1081 acgtcgtcgt cgccgcggcg aatccgcttg tcgcgcaacc cgagcgcgtc gatcgtgcgg
     1141 ccgccttcga gagtctcggt aatgccggcg aacatgtgcg agtagacgcg gcgctcacgc
     1201 aggtagccct gcggcgcgcg cttgaggtac caccgcgtcg cgatgaagag caacggaaac
     1261 acgatcaggc atggcaaggc gaccagtggc gcagtaatga atgcagcgat caaagtgagc
     1321 accccggtcg caatcgcgat catcgtttcc ggcaccgcga accgcacgct gcgcgacatc
     1381 gcgtcgatgt cgctggtcat gcgggttagc aggtcgcccg ttccggcgcg ctcgacggtc
     1441 gacagcggca gcgagatgac cttgcgcagg aattcctctc gcagctcggc gaaaaccttc
     1501 tccgcaatga cggacgacgc gtagcgcgcg agccgtacga aaatcgcctg cgctacaacg
     1561 aatccggcca ggatcatggc gatccggtcg acgtgcgccc gcgtcgtccc tttctgtacg
     1621 gcgccgacga tgtcgccgag aaggcgtggc ccggccaagc cggcggcgcc agcgagcgcg
     1681 tacaggacca gcacgccacc cgccgcgcgg ccctgcttgc gaatgagcgt ggccgcgtac
     1741 gccttcacct cggcgatatc ggcgacggga agtagcgcgg agttcgacgc gctcatgcgg
     1801 attcctcgtc gcgggtgacg acggagcgat agcgcgcatc gtgcgacacg agctcgcggt
     1861 gccgcccatc ggcgacgaca cggttgcctt cgagcagcac agcgcggtcg gcgcggtcga
     1921 gcatcaacgg gctggtcgac atgacgatcg tggtcttgcc cgcgcgatgc gcgtgcaggg
     1981 tgtccgcaat ccgcgcttcg gtataggcgt cgacggcact cgtcggttcg tcgagtacga
     2041 ggacgggcgc atcgacaagg agcgctcgag cgagtgcaat tcgctgtcgc tgcccgcccg
     2101 agaggccaag tgcgcgttcg tcgagtacgc catcgaggcc gccgggcacg gactcgacgg
     2161 cgtcatgcgc cactgcggca tcgatgacgt cgtcgacctc ggcggcacta gctcggcggt
     2221 gcgggtcgag ctggtctcgc agtgaacccg cgaaaagacg ggggtccttg tcactgatca
     2281 ggatggtcgc ccggacgtcg tcgagcgcca tcgcggtaag tggcacgttg ccgaagagca
     2341 cccgctc
//
LOCUS       DATCBJ010000550         3953 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_529922, whole genome shotgun sequence.
ACCESSION   DATCBJ010000550 DATCBJ010000000
VERSION     DATCBJ010000550.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 3953)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3953)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3953
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_529922"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..234)
                     /locus_tag="VII50_11040"
     CDS             complement(<1..234)
                     /locus_tag="VII50_11040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5051427.1"
                     /translation="MPIPRYGSASLADLVPSVLASLGTPTFRGVLSLPPAKAACILLI
                     DGLGAELIRAHAAEAPLLHAALTGDAGRDLTSGF"
     gene            complement(262..861)
                     /locus_tag="VII50_11045"
     CDS             complement(262..861)
                     /locus_tag="VII50_11045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5998 family protein"
                     /protein_id="HEY5051428.1"
                     /translation="MPDNTTADSAQSELWADVDRSGYYPELMADSLRGTLGTEQALAH
                     VVHQETTLDDAMGIRRHVTVLATTPTRLIVCHTDEHPASELTPYPHATTTTEVVPLSR
                     IASVALTTMVANPAEYLPGASYREATLVVGWGAISHVDLEPATCGDETCDADHGYSGT
                     LTADDLSLRVSQDGDGGPVVTQAIRFATVLSEAAARARA"
     gene            complement(898..1668)
                     /locus_tag="VII50_11050"
     CDS             complement(898..1668)
                     /locus_tag="VII50_11050"
                     /inference="COORDINATES: protein motif:HMM:NF012692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEY5051429.1"
                     /translation="MPNLFGLDIGGSGIKGAPVDLARGVLVGERVRLVTPAGAKVPEI
                     AKTAAEIVASFGYTGLAGAAFPAVIIDGHARNASNIDAGWIGTDVAGTLSDATGCDVR
                     VLNDADAAGLAEMRYGAGRDVGGTVALLTLGTGIGSALFLDGELVPNTELGHLEIDGQ
                     DAELKTAESARDREDLSWEHWARRVSKYLRHFEALLWPDLFILGGGVSKKADKFLPLL
                     EGVRTKVAVAQLQNDAGIIGAALWASQARGGAAQARGG"
     gene            complement(1668..2795)
                     /locus_tag="VII50_11055"
     CDS             complement(1668..2795)
                     /locus_tag="VII50_11055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HEY5051430.1"
                     /translation="MAGAQDSNAQDTRGTRRPRKTAAKPPGGRVAFPGISSRAYEHPA
                     DRAALVTLRGVPGFDVLLKGMFGFFSERRLRMMYLASAVRVDERQFPAIYAIYQDAAR
                     ILDITKVPELYINQEPRVNAWALGLDNPFIVVSTGTVDLLDDDELRVVLGHELGHVLS
                     GHALYSSVLFALMRISGVGNAIPLGGLGLRAVVTALKEWYRKAELSCDRAGLLVSQDP
                     GAVTRVHMKMAGGARVQDMNLQAFLAQAEEYESTGDAREGVIRILNILNSTHPFAVLR
                     ALEIKRWVESGDYERILAGNYPNRADDPRASFVDEVKAAAASYKQSFDRSPDPLMKFL
                     RDVGGGTVNLGGWVADQVRKMAGGSNHDDDGDNHDDHDAQA"
     gene            complement(2806..>3953)
                     /locus_tag="VII50_11060"
     CDS             complement(2806..>3953)
                     /locus_tag="VII50_11060"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEY5051431.1"
                     /translation="GTDPYKCRGATRYVDAVAAPAEHPLVETARRLAEELLEPAAAQV
                     DASTVPRSHLQAMADAGLLGMVAPAEVGGGGASAPVFREVTEVLAGADAATWFVQAQH
                     QGVVRMLAGNAGPAAVRYLPRLATGELIAGFAFSHLRRFPERPVTARRVAAGWRLDGV
                     APWYTGWGLNDVAFISGVTDDGDVVFGAVPAVEGGGLRMKTRLRTAALDAACTVVLAL
                     DGVTVPDDDVALQQPHAQWSAADAGAADANPAIFGVAASALRLLGDAGAAREELATTQ
                     AAVVLGQRIADVRTRCYALADAVPLGEAIDERLALRAVALELLIAATSALVAANSGPA
                     MALTSPAQRKAREALFLLVQAQTASARAATLNRWSASAAATAPQTRA"
BASE COUNT          665 a         1405 c         1285 g          598 t
ORIGIN      
        1 aaacccagac gtcaagtcac gtccggcgtc gccagtgagc gccgcgtgca gcaacggtgc
       61 ttcagctgcg tgtgcccgga tcagttcggc gccgagaccg tcgatgagca ggatgcatgc
      121 cgccttcgcc ggcggtagcg aaagcacacc gcgaaacgtg ggagtgccga gcgacgccag
      181 cactgatggc accagatcgg cgagggatgc ggatccgtac cgtgggatcg gcaccgagcc
      241 tggatggacc gcggtcaccg gtcaggcccg cgcccgcgca gccgcctcgg acaaaacggt
      301 cgcgaacctg atcgcctgcg tgaccaccgg gccgccatcg ccgtcctggc tcacccgcag
      361 cgagagatcg tcagcggtga gcgtgcccga atagccgtgg tcggcgtcgc acgtctcgtc
      421 accgcacgtc gccggctcca ggtcgacgtg actgatcgca ccccaaccca cgacgagagt
      481 ggcttcccga tacgacgcgc caggcaggta ttcggcggga ttggccacca tcgtcgtcag
      541 cgcgaccgaa gcgatgcgcg acaacggcac gacctcggtt gtggtcgtcg cgtgcgggta
      601 aggcgtgagc tcgctcgccg gatgctcgtc ggtgtggcac acgatcaggc gggtgggcgt
      661 tgtcgccagc accgtgacgt gccgccggat gcccatcgca tcgtcaagcg tcgtctcctg
      721 gtggacgacg tgagcgagcg cctgctcggt accgagcgtg ccgcgcagcg aatcggccat
      781 gagctcgggg tagtagccgc tgcggtcgac gtccgcccac agctctgact gggccgagtc
      841 ggccgtggtg ttgtcgggca tggctccatc ctccactatc gcgggccgcg atggcggcta
      901 gccgcctcgt gcctgcgccg ccccgcctct tgcctgcgac gcccacaggg ccgcgccgat
      961 gatgccagcg tcgttttgca gttgggcgac cgcaaccttc gtgcgaactc cctcgagcag
     1021 cggcaagaac ttgtccgcct tcttgctcac gccgccaccg aggatgaaga gatccggcca
     1081 caagagggcc tcgaaatggc ggagatattt cgacacccgg cgggcccagt gctcccagct
     1141 gaggtcttcg cggtcacgag ccgactcggc ggtcttcagc tcggcatcct ggccatcgat
     1201 ctccagatgc ccgagttcgg tgttgggcac cagttcgcca tcgaggaaaa gcgcgctacc
     1261 gatacctgtg ccgagcgtca ggagcgccac ggtgcccccg acatcgcggc cggcgccata
     1321 ccgcatctcc gcaagaccag ccgcgtcggc gtcattgagc acccgcacgt cgcaaccagt
     1381 cgcgtccgag agcgtgcctg ccacgtcggt accgatccag ccagcgtcga tgttgctcgc
     1441 attgcgcgca tgcccgtcga tgatcacggc gggaaacgcc gctccggcga ggcccgtgta
     1501 gccgaagctc gcgacgatct cagccgccgt cttggcgatc tcgggcacct tcgcaccggc
     1561 gggcgtcacc agccgcaccc gctcaccgac caacacgccg cgggccagat cgaccggcgc
     1621 acccttgatc cccgagccac cgatatccag tccgaacagg ttaggcatca ggcctgcgca
     1681 tcgtggtcgt cgtgattgtc gccgtcgtcg tcgtgattgc tcccgccggc catcttgcgg
     1741 acctggtcag cgacccaacc accgagattc accgtgccgc cgccgacgtc acgcaggaac
     1801 ttcatcagcg gatccggcga tcgatcgaac gactgcttgt acgacgcggc cgcagccttc
     1861 acttcgtcga caaatgacgc gcgcggatcg tcggcgcggt tggggtagtt gcctgcaagg
     1921 atgcgttcgt aatctcccga ctcgacccac cgcttgatct ccagtgcgcg cagtaccgcg
     1981 aacgggtgcg tcgagttgag gatgttcagg atccggatga cgccttctcg cgcatcgccg
     2041 gtcgactcgt attcttcagc ctgcgcaagg aacgcctgca ggttcatgtc ctgaacacgc
     2101 gcaccgccgg ccatcttcat gtggacgcgc gtgacagcgc ccgggtcttg actcaccagc
     2161 agacccgcgc gatcacacga gagttccgcc ttgcgatacc actctttcag cgcagtaacg
     2221 accgcacgaa ggccgaggcc gccgagcggt atcgcgttgc ccactccgct gatgcgcatc
     2281 agcgcgaaca acacggacga gtagagcgca tggccggaca gcacatggcc gagttcgtga
     2341 cccaacacga cacgcaactc gtcgtcgtca agcagatcga cggtgcccgt actcacgacg
     2401 atgaaagggt tgtcgaggcc caacgcccag gcgttgaccc gcggctcctg gttgatgtac
     2461 agctcgggca ccttggtgat gtcgaggatg cgcgcggcgt cctgatagat cgcgtagatg
     2521 gcgggaaact gccgctcgtc gacccgcacg gccgaggcga gatacatcat gcgcaagcgc
     2581 cgttcgctga agaagccgaa catgcctttg agtaggacgt cgaaccccgg caccccgcgc
     2641 agcgtgacca atgccgcgcg atccgccggg tgctcgtagg cgcgtgaact gatgccaggg
     2701 aaggcgacgc ggccacccgg tggcttggca gcagtcttgc ggggacggcg cgtgcccctc
     2761 gtatcctgcg cgttcgaatc ctgcgcacca gccatgtccg ccacgctacg ccctggtctg
     2821 cggcgcggtg gccgccgcac tagccgacca ccggttcagc gtcgccgcgc gagccgacgc
     2881 ggtctgcgcc tggaccagga ggaacagcgc ctcgcgtgcc ttgcgctggg caggtgaggt
     2941 gagcgccatc gctggcccgg agtttgcggc gaccagcgcc gaagtcgcag caatcagcag
     3001 ctcgagagcc accgcgcgca acgcgaggcg ctcgtcgatt gcctcgccga gcggtaccgc
     3061 atcggccagt gcgtaacacc gcgtacggac gtcggcgatc cgttggccca acacgactgc
     3121 ggcctgcgtg gtcgcgagct cctcacgcgc agcgccagcg tcgcccagca agcgcagcgc
     3181 gctcgcggcg accccgaaga tcgccgggtt ggcgtccgcc gcacccgcgt ccgccgccga
     3241 ccactgggcg tgcggctgct gcagcgcgac atcgtcgtcg ggcacggtga cgccgtcgag
     3301 tgcgagcacg accgtgcagg ccgcgtcgag cgcagcggtc cgcaagcgcg tcttcatcct
     3361 cagcccgccg ccctcgaccg cggggacggc accaaagacc acgtcgccgt cgtcggtcac
     3421 gcccgaaatg aatgcgacat cgttcagccc ccaccccgtg taccagggcg ccaccccatc
     3481 gagacgccaa ccggctgcta cccgacgcgc ggtcaccggc cgctcaggga atcgacgcag
     3541 atgggagaac gcgaatccgg cgatcaactc gcccgtcgcc aaccgcggca ggtaccgcac
     3601 cgcagctggc ccggcgttgc cggccaacat gcggacgaca ccctggtgct gagcctgcac
     3661 gaaccaggtc gcggcgtcgg cgccagcgag cacctcggtg acctcgcgaa agaccggggc
     3721 cgaggcgcca cctccgccga cctcggccgg cgcgaccatc ccgagcaaac cggcgtccgc
     3781 cattgcttgc aagtgggagc gcggcacagt tgacgcgtcg acctgcgcgg ctgcgggttc
     3841 gagcagctcc tccgcaagtc ggcgggcggt ctcgaccagc ggatgctcgg cgggcgcggc
     3901 caccgcgtca acgtagcggg tcgctccccg acacttgtac gggtcggtcc ccg
//
LOCUS       DATCBJ010000551         3042 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_14282, whole genome shotgun sequence.
ACCESSION   DATCBJ010000551 DATCBJ010000000
VERSION     DATCBJ010000551.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 3042)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3042)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3042
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_14282"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..196
                     /locus_tag="VII50_11065"
     CDS             <1..196
                     /locus_tag="VII50_11065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5051432.1"
                     /translation="PGEATRDVFPGSLVMSGGKVVPSDAPGWGIGFDEEQAAHHEPIS
                     GAKRTFLEFRRRANGTIQHP"
     gene            complement(317..628)
                     /locus_tag="VII50_11070"
     CDS             complement(317..628)
                     /locus_tag="VII50_11070"
                     /inference="COORDINATES: protein motif:HMM:NF015925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HEY5051433.1"
                     /translation="MWAQLIKMRLKPGSDADIPALFQQFAAVEQTGSGLIRTTLMHAQ
                     NDPSQFYVMVVFESEEQARARERDPARQEALKSAQAMMVEIFDGPPEFVDLTVVADMV
                     P"
     gene            complement(689..1117)
                     /locus_tag="VII50_11075"
     CDS             complement(689..1117)
                     /locus_tag="VII50_11075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009079523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY5051434.1"
                     /translation="MYKIANTQLWVHDQDEALAFYTTKLGMEVRSDVTMAEMGNFRWL
                     TVGPVDQPDFAIVLMAVPGAPVMDEQTSDEVRQLMAKGFAGTVFLTTDDCRASYENLR
                     ARGVEFTEAPEERPYGIDAGFRDPSGNSFRLTQVADFVNA"
     gene            complement(1080..1616)
                     /locus_tag="VII50_11080"
     CDS             complement(1080..1616)
                     /locus_tag="VII50_11080"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEY5051435.1"
                     /translation="MSANARAYSTTQIPIARHLLRAKDLADARYAEPLDVATLATAAG
                     LSPAHFSRAFRKAFGESPHAYLLTRRLERAAALLRNTDNSVADICVAVGLSSIGSFTT
                     SFRRMFGATPTAYRASFPPAAAFARVPACVVRAYSRPLLRTFREDGKVPGSVASGTPT
                     PDPGGRHVQDRQYPAVGP"
     gene            complement(1616..2827)
                     /locus_tag="VII50_11085"
     CDS             complement(1616..2827)
                     /locus_tag="VII50_11085"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013253.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy acid oxidase"
                     /protein_id="HEY5051436.1"
                     /translation="MADRQVPKWRELKPLLRPKPFDPNPTRRRLAAALTIADLRLIAR
                     RRTPRSVFDYTDGAAGDEISLRRARALFRDVEFQPSILRDVSGADMTTHILGKPSALP
                     FALAPTGFTRLMHHEGESAVVRVAARRDIPYALSTMGTTSIENVAAAAPDARKWFQLY
                     VWRDRAAAEDLMSRAKAAAFEALILTVDVPVAGPRLRDVRNGFSIPPALTVKTVLDAA
                     THPAWWLNLLTTPPLTFASLTESGGTIAELIDALFDPSMTISDLEWLRSFWDGPLIIK
                     GIQTVDDARRVVDAGADAVVLSNHGGRQLDRAPVPLRLIPDVLDAVGDRTEVWADTGI
                     MAGADIVAAIALGARATLIGRAYLYGLMAGGERGVDRAVEILSREVRRTMQLLGVRTI
                     DELSPAHVKLP"
BASE COUNT          527 a          970 c         1028 g          517 t
ORIGIN      
        1 gccaggtgaa gcgactcgag acgtatttcc gggcagcctc gtcatgagcg gcggcaaggt
       61 cgtgccgagc gatgcgcccg ggtggggtat cggattcgac gaggagcagg cggcccatca
      121 cgagccgatc tcgggtgcga agcgcacgtt ccttgagttt cgtcgacgtg caaacggaac
      181 catccagcat ccgtagtcgc acgttggtgg ggcgacagac cgaccgtcca acgccggcgc
      241 cgagccgcgg tcgtcgtaat cgaagcggac gccgtggccc tcgacgacgg gcacgcgact
      301 ccccaggctc gcgtgcctac ggcaccatgt ccgcgacgac cgtgaggtcg acgaactcag
      361 gcggaccgtc gaatatctcc accatcatcg cctgcgcgga cttcaatgcc tcctggcggg
      421 ccgggtcgcg ttcgcgcgcg cgagcctgtt cctcactctc gaatacaacc atgacgtaga
      481 actggcttgg atcgttctgc gcatgcatga gcgtggtccg tatcaagccg gaacccgttt
      541 gctcgacggc ggcgaactgc tggaacagcg ccggtatgtc cgcgtcgctg cccggcttca
      601 gacgcatctt gatgagctgt gcccacatgc cagaacccct tgcgctggag tagcgattgg
      661 ggctgagcct agcgccgttt ctggcgggct aggcgttgac gaagtcggct acctgggtga
      721 gtcggaagct gttgcctgag gggtctcgaa atcctgcgtc gattccatac ggacgctcct
      781 cgggcgcctc ggtgaactcc acgccgcgtg ctcgaaggtt ctcgtatgac gcccggcagt
      841 catctgtggt gaggaagacc gtgccggcga atcccttggc catgagctgg cgaacctcgt
      901 cggatgtctg ctcgtccatc acaggcgcgc cggggacagc catcagaacg atcgcgaagt
      961 ctggctggtc caccggaccc acggtcagcc agcggaagtt gcccatctcc gccatggtga
     1021 cgtcagaacg cacctccatg ccgagcttgg tcgtgtagaa cgcgagcgcc tcgtcctggt
     1081 catggaccca cagctgggta ttggcgatct tgtacatgtc gtcctccggg atcaggtgtc
     1141 ggtgtacccg acgctactga ccccggaacc ttcccgtctt ctcgaaacgt gcgcaacagt
     1201 ggccggctgt acgcgcgcac cacgcacgca ggcacgcggg cgaaggcggc ggccggcggg
     1261 aaagacgcgc gataggcggt aggggtggca ccgaacatcc gccggaagct cgtcgtgaac
     1321 gaaccgatgc tcgacaagcc gaccgcgacg cagatgtccg cgaccgagtt gtcggtattg
     1381 cgcaagagcg cagctgctcg ctcgaggcga cgcgtcaaca gatacgcgtg aggtgactcg
     1441 ccgaacgcct tacggaacgc gcgactgaaa tgcgccgggg acagtcctgc ggcggttgcg
     1501 agcgtcgcca cgtcgagcgg ctcggcgtac cgcgcgtcgg ccagatcctt ggcgcgcagc
     1561 aggtgtcggg cgatcggtat ctgagtcgtt gagtacgccc gcgcattagc cgacatcagg
     1621 gcagctttac gtgtgcaggg ctcagttcgt cgatcgtccg tacgccgagg agttgcatgg
     1681 tccggcgcac ctcgcgcgac aggatctcca ccgcacgatc gacgccgcgt tcgccacccg
     1741 ccatgaggcc gtagagatat gcgcggccga tgagggttgc tcgggcaccg agggcgatcg
     1801 cggccacaat gtccgcgccg gccatgatgc cggtatcggc ccagacctcg gtgcgatccc
     1861 cgacggcatc gagaacgtcg gggatcaggc gcagtggcac cggcgccctg tcgagttgac
     1921 ggccaccgtg attggacagc acgacggcgt ccgctcctgc gtcgacgacc cggcgcgcgt
     1981 cgtcgacggt ttggatgccc ttgatgatga gtggcccatc ccagaacgag cgcaaccatt
     2041 cgaggtcgct gatcgtcatc gacggatcga acagggcgtc gatgagttcg gcgatggtgc
     2101 cgccggactc cgtgagagaa gcgaacgtga gcggaggggt ggtgagcaga ttgagccacc
     2161 acgccgggtg cgtcgccgca tcgagaaccg ttttgacggt gagtgcgggc gggatcgaga
     2221 agccgttgcg tacatcgcgc aaccgaggac cggcgacggg gacgtcgacg gtgagaatca
     2281 gcgcctcgaa cgcggccgcc ttcgcgcggc tcatcaggtc ttccgcggcg gcccgatctc
     2341 gccacacgta gagttgaaac cacttccggg cgtcgggcgc ggcggcggcg acgttctcaa
     2401 tagacgtggt tcccatcgtc gacaacgcgt agggaatgtc acgacgcgcc gcgacccgca
     2461 cgaccgcgct ctcaccttcg tgatgcatga gccgggtaaa gcccgtcggg gccagcgcga
     2521 acggcagcgc tgagggcttg cccaggatgt gggtggtcat gtcggcgccc gacacgtcgc
     2581 gcaggatcga cggctggaac tccacgtccc tgaagagggc acgtgcgcgc cgcaagctga
     2641 tctcgtcgcc ggcggcgccg tcggtgtagt cgaacaccga ccggggagta cgccggcggg
     2701 cgatcaaccg aaggtccgcg atcgtcagcg ccgcggccag ccgtcgccgg gtcggattgg
     2761 ggtcgaacgg cttcgggcgc aatagtggct tgagctcgcg ccacttcggt acttgtcggt
     2821 cagccacgat ctcgactcca acgggtctgg cggaaacctg cgtccggcgt aatcaggtcg
     2881 cctcgccgtc ggcggcccgt cgcagttcgt tgatgtcgat cttgctcatg ccgagcatcg
     2941 ccttcatcgc gcgttccgca cgtgccggat cgggatcccc gaagaactct tccatgccac
     3001 ttgggacaac ctgccaggag aggccgaact tgtccttgag cc
//
LOCUS       DATCBJ010000552         2870 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_455466, whole genome shotgun sequence.
ACCESSION   DATCBJ010000552 DATCBJ010000000
VERSION     DATCBJ010000552.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2870)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2870)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2870
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_455466"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(83..1951)
                     /locus_tag="VII50_11090"
     CDS             complement(83..1951)
                     /locus_tag="VII50_11090"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HEY5051437.1"
                     /translation="MISPPSGDALLAMLLGLLNESDDLDRTLQASLDLMTTSLGGRIG
                     EIWLRSGDARQVELHYSSSDGSPAVVAFDSVGRSLGLGLGPSLVSRVVKTGRGSTLPP
                     ITAGGMEERNVEALAADIHSALTFPIRTSRGVIGVLVVFREPTERPIEGALAAIPVAC
                     HHIGHFLERVRAESAFHATALELSALASTDVLTGLKNRREFDRSLRTIPRQPFAVLSL
                     DVDGLKATNDAHGHAAGDTLLRSVGHTLGLLVRGWDVMARVGGDEFAALLPEVGVFGA
                     NLVAERMRTAMHALVLPSGPVRITVGWSTAPAGADPVSVWQKADESLYKAKHAGGDRV
                     TGGAFEGGEAVDIAERSYSDVVTRIFEGGALSTMFQPIVNLFDGSVTGYEALARPEGF
                     AAMDSVEAVFEAARTSGRIRDLDWVCRRRAVEDAQLLPLGSTLFLNISAAALLDPLHD
                     VDQLLLLLRSVERAPQTVVLEITEHERIRDYELLGRVLSSYRTEGIRFALDDVGEGHS
                     TLELLAASSSEYLKLGRSLTMTSGRTGSRAAIDATLAFAHVTGSTVIAEGVENEFVAD
                     QMKAGGIRLAQGFGLGRPTLAAEVEDVGAALSDRAALSGLRPRSTSLSAMGRHLPR"
     gene            2363..2500
                     /locus_tag="VII50_11095"
     CDS             2363..2500
                     /locus_tag="VII50_11095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5051438.1"
                     /translation="MPTEQAPTLTKRTHRWRSPANYYAVLFWVVIIVVVIVFAWHPWH
                     G"
BASE COUNT          544 a          931 c          909 g          486 t
ORIGIN      
        1 acgggcagtt gcaaagcccc atgcgctgat tgacccggtc tctaacctca caccatcagc
       61 ggcgacgcag tcattgcacc gcctaccgcg gaagatggcg gcccatcgcc gaaagcgagg
      121 tcgagcgcgg acgcagccca ctgagcgcgg cacgatcgga gagcgccgcg ccgacatcct
      181 cgacctcagc ggccagcgtg ggtctgccca gtccgaagcc ctgcgccaat cggatgcccc
      241 ctgccttcat ctgatcggcc acgaactcgt tctcgacgcc ctcggcaatg acggtagacc
      301 cagtaacgtg tgcaaaggcg agcgtcgcgt cgatggcagc ccgtgagccg gtccggcccg
      361 acgtcatcgt gagactgcgc cccagcttga gatattcgct cgaagaggcg gcgaggagct
      421 cgagtgtgga gtgaccttcg cccacgtcgt caagcgcaaa ccggatgcct tcggtgcgat
      481 acgaggacag gactcgcccc agcagctcgt agtcgcgaat gcgctcgtgc tcggtgatct
      541 cgagcacgac cgtctggggc gcgcgctcga ccgagcgcaa caggaggagg agctggtcga
      601 cgtcatggag gggatcgagc agagctgccg cgctgatgtt caggaacagc gtacttccca
      661 agggaagcag ctgcgcatcc tcaacggctc tgcgccggca tacccagtcg aggtcgcgaa
      721 tccgaccact cgttcgtgca gcctcgaaca cagcctccac cgagtccatg gccgcgaatc
      781 cttcaggccg cgccagggct tcgtagccgg tcaccgaacc atcgaacaga ttgacgattg
      841 gctggaacat ggtcgagagc gctccgcctt cgaagatacg cgtgaccaca tcggaatagg
      901 accgctcggc aatgtcaacg gcttcgccac cttcgaaggc gccgccggtc acccggtcgc
      961 cgcccgcatg tttggccttg tacagcgatt cgtcagcctt ctgccacacc gagacggggt
     1021 cggcgcccgc gggcgccgta ctccagccca cggtgatacg gaccggaccg ctgggcagca
     1081 cgagcgcatg catcgccgtc cgcatgcgct ccgccaccag gtttgcaccg aagacgccga
     1141 cctcaggcag caacgccgcg aactcgtcgc cgccgacgcg tgccatcacg tcccatccgc
     1201 gtacgaggag tcccagggtg tgtccgacac tgcgcagcaa tgtgtcgccg gcggcatgac
     1261 catgggcgtc gttggttgct ttgagcccgt cgacatccag agagaggacg gcgaaaggct
     1321 ggcgcgggat ggtgcgtaag gaccgatcga attcgcgcct gtttttcaga ccggtaagca
     1381 catccgtaga cgccaacgcg gagagttcga gggccgtcgc gtggaacgca ctttcggcgc
     1441 ggacgcgctc gagaaagtgc ccgatgtgat gacaggccac agggatggct gcgagcgccc
     1501 cctcgatggg gcgctccgtg ggttcccgaa agacgaccag gactccgatc acaccgcggc
     1561 tggtccggat cgggaaggtc agcgcgctgt ggatgtccgc ggccagcgcc tcgacattgc
     1621 gctcttccat tcccccggcc gtgatcggag gcaacgtcga tccgcgtccg gtcttcacaa
     1681 cgcggctcac gagcgacggc cctagaccga gtccgagcga cctgcccaca gaatcgaatg
     1741 caacgaccgc cggggatcca tccgaggacg agtagtgcag ctcgacctgc cgagcatcac
     1801 cgctgcgcaa ccagatctca ccgattcggc cacccagcga cgttgtcatc agatcgaggc
     1861 tcgcctggag tgtcctatcc aggtcatccg attcgttgag caggcccagc agcattgcga
     1921 gcagcgcatc accactcggc ggggagatca cggcgagctg ttcgacagct cctccagcgg
     1981 ccgcacagac gcggctcctc gatccgcgac gtgccgtcgg aatgagtgtc gtcgggacgg
     2041 tgggtcggtc cgttcgcgat gtggacacag ctgagttgcc tcgggagcct gagtacttcc
     2101 tgccgccacg atcttcgcgc tctgccgtga ggtctgcgcg ctaccgcaca tagtggatag
     2161 tccgcgacaa gcgtgacccg atcgagtcac gagcccggta gccgcatgcg ggcaatgagg
     2221 caccgatcga tgtgaccaag cacatccgcc ggtgtgcgat gacgtctagt aacacggcca
     2281 ggaagagcca gcgtctcgcc acaagcaccg acggcattgt cacgagcgtg cgccagcgat
     2341 ttggtgaacg taaggtgctt tcatgccgac agagcaggcg ccgacgctta ccaaacgcac
     2401 tcatcgatgg cggtccccgg cgaactacta tgcagtgctc ttttgggtcg tgatcatcgt
     2461 cgtcgtcatc gttttcgcgt ggcacccatg gcacggctag gtcgtcgtga cgcccacgtc
     2521 ggatggattc gcggcggccg ggccgtaatt agaattcaca agtcatgtcg cgcttggaga
     2581 cgctcaccac cgcaggcctc ctggagttgt accgagacac acttcgtgag ttgcgggacc
     2641 gcggggtcat aaggacggcg aatgccccgg cgggtgacta tgccgagtac atagtgcgcc
     2701 gcgctttccc cggcagcgag ctggctccga actcagagaa gagctgggat gttaaggcag
     2761 cggatggcac gcgactacag gtcaagtgtc gagtgatctt cgacctcagg aatcgcgggc
     2821 agcgtcagtt gtctccgttt cgctcgtttg ggttcgatgc tgcggccatc
//
LOCUS       DATCBJ010000553         2096 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_432924, whole genome shotgun sequence.
ACCESSION   DATCBJ010000553 DATCBJ010000000
VERSION     DATCBJ010000553.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2096)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2096)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2096
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_432924"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1198
                     /locus_tag="VII50_11100"
     CDS             <1..1198
                     /locus_tag="VII50_11100"
                     /inference="COORDINATES: protein motif:HMM:TIGR03026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HEY5051439.1"
                     /translation="TPPFDRDVTIIGGCGHVGLPLGIALASRGLLVTLVDISRSAVDI
                     VNAGSLPFREPGAEQLVKDLTIAGSLRATTDPAAIRAAENVIVVTETPLDEHLNPDPA
                     VFLAAMGGARPYLRDGQLVILRSTVYPGMTKEVELMIEAADLDVEVAFCPERIAEGYA
                     MHELFSLPQIVGARTHRVRARAGKLFANLTGSIVEVEPEEAELAKLFSNAWRYIKFAA
                     ANQFYYIANDYGQDFERIRQALAADYPRAADLPRAGFAAGPCLFKDTAQLAAFDRSSF
                     AIGNAAMLVNEALPLYVVDRLEQRFDLRSMTVGVLGMAFKGESDDIRASLSYRLKAIL
                     EPRARAVLCTDPYVTVDPTLVPLDRVLADCDLLVVATPHEAYRRLHPIQPVADVWNVL
                     GLGVVI"
     gene            1198..2019
                     /locus_tag="VII50_11105"
     CDS             1198..2019
                     /locus_tag="VII50_11105"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEY5051440.1"
                     /translation="MPRRVAAGPRVSIVIPALNEGDDIVPCLDRILEAVALPCEILVV
                     VDDSADSTCAAVEGVFATERTVHVLVNDRGRGPGQAIRYGIDHARAPVVVVTMADGSD
                     DPRLIDDLTRLVERGVVVAAASRYSPGGQQVGGPALKGFLSRFAGRSLYFAARVGTRD
                     ATNSFKAYSTAFVREVGIDSRHGFELGLELTAKARRLRRPIAEIPTIWLDRAAGVSNF
                     KVATSIPDYLFWYRFAFGRSFHLETMNRKLTRSRLRLTEVARAEPHNGRVRRSGD"
BASE COUNT          348 a          667 c          664 g          417 t
ORIGIN      
        1 cacgccgccg ttcgaccgcg atgtcacaat catcggtggc tgcggccatg ttggactgcc
       61 gctcggtatt gcgttggcgt cgcgcggcct gctggtgacc ctggttgaca tctcgcgatc
      121 cgccgtcgac atcgtgaacg cgggcagtct gccgtttcgc gaaccagggg ccgagcagtt
      181 ggtcaaagac ctgacgatcg ccggttctct tcgagccacc accgacccag ccgcgatccg
      241 ggctgccgag aacgtcatcg tggtaaccga gacgcccctc gacgagcatc tgaaccccga
      301 tccggcagtg ttcctggcgg cgatgggcgg cgcgcggccg tacttgcgcg acggtcaact
      361 cgtcattctt cgcagcaccg tctacccggg catgaccaaa gaggtcgagc tgatgatcga
      421 ggcggcagat ctcgatgtcg aagtcgcgtt ctgccccgag cgcatcgcgg agggctacgc
      481 gatgcacgaa ctcttctcgc tgccgcagat cgtgggggca cgcacgcacc gtgtgcgcgc
      541 gagggccggc aagctgttcg ccaacctcac gggctcgatt gtcgaggtcg agcctgagga
      601 agccgagctt gccaagttgt tcagcaatgc gtggcggtac atcaagttcg cggcggccaa
      661 ccagttctac tacatcgcca acgattacgg tcaggacttc gagcgcatcc gacaggcgct
      721 ggcggccgac tatccgcgtg ctgctgacct gcctcgcgcc ggcttcgcgg ccggaccctg
      781 cctgttcaag gacacggcac aactcgccgc gttcgacagg agctctttcg cgatcgggaa
      841 tgccgccatg cttgtcaacg aagccctgcc gctctacgtc gtcgaccggc ttgagcagcg
      901 cttcgatttg cggtcgatga ccgtgggcgt tctgggaatg gcgttcaagg gcgagtccga
      961 cgatatcagg gcgagccttt cgtacaggct taaggcgatt cttgagccca gggcacgtgc
     1021 ggtgttgtgt accgatccat acgtcacggt cgacccgacc ctggtgccgc tcgaccgggt
     1081 ccttgccgat tgcgacctcc tcgtcgtcgc gacaccgcac gaagcgtacc gccgccttca
     1141 tcccatccaa cctgtcgccg atgtatggaa cgtcctcggc ctcggcgtgg ttatctgatg
     1201 cctcgccgcg ttgctgctgg gcccagagtc agcatcgtca ttccggcgct gaatgaaggt
     1261 gacgacatcg tcccgtgcct cgaccgcata cttgaagcag tggcgttgcc ctgcgagatc
     1321 cttgtcgttg tcgacgactc cgccgactcc acctgtgcgg ccgtcgaggg tgtcttcgcg
     1381 actgagcgca cagtccacgt tctagtcaac gaccgcggcc gtggcccggg acaagcgatc
     1441 cgttacggga tcgaccacgc gcgcgcgccg gtcgttgtgg tcaccatggc tgatggcagc
     1501 gatgatccgc ggttgatcga cgatctgacg cggctggtcg agcgcggcgt cgtcgttgcc
     1561 gcggcctcgc gttactcccc aggtggtcag caggtcggtg ggccagctct taaaggtttt
     1621 ctctcccggt tcgcggggcg ttcattgtat ttcgcggcgc gagtcggcac tcgtgacgca
     1681 accaactcgt tcaaggccta ctcaacggcc ttcgtacgcg aggtcggtat cgacagccgc
     1741 cacggattcg agctcggcct cgagctcacc gcgaaggcac ggagattgcg ccgaccaatt
     1801 gccgaaatac cgacgatctg gcttgaccgg gcagctggtg tttccaactt caaggttgca
     1861 acgtcgatcc cggactattt gttctggtac cggttcgctt tcggtcggtc gttccacctc
     1921 gaaacgatga acaggaagct cacaaggtcg cggctacgtc tgaccgaggt cgccagagcc
     1981 gaaccccaca acggacgggt gcggcgcagt ggcgattgag caggtaatgg tgtcgggatc
     2041 cgcgggattc atcggcgggt atgtcgtcga ggagctgttg agccgcggct atgccg
//
LOCUS       DATCBJ010000554         3539 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_517308, whole genome shotgun sequence.
ACCESSION   DATCBJ010000554 DATCBJ010000000
VERSION     DATCBJ010000554.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 3539)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3539)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3539
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_517308"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..919)
                     /locus_tag="VII50_11110"
     CDS             complement(<1..919)
                     /locus_tag="VII50_11110"
                     /inference="COORDINATES: protein motif:HMM:NF012742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEY5051441.1"
                     /translation="MPRQITAVGAISLLNEEMRVPQVRQHQADQQPPPAVPGSKQRRL
                     GNLALPAGLRAAGSRPAMADVARIAGVSHQTVSRVVNEVDRVRPATRARVRAAIEQLG
                     YLPNVAARALATGRTRTVGVVTVASRLFGPASTLHGIEQAARAEGYFVTMVSLSSIDP
                     GSVREAIGRLVAQGVDGVIVDVPLVSALGAIGQLSVDIPIVAVEGDPLGGVPVVSVDQ
                     VDGARQATEHLLSAGHATVWHVAGPNDWLETHGRIAGWRAALVSAAAHVPPLLHGDWS
                     AKSGYEAGRILASVDKVTAVFVANDQMALG"
     gene            complement(873..2492)
                     /locus_tag="VII50_11115"
     CDS             complement(873..2492)
                     /locus_tag="VII50_11115"
                     /inference="COORDINATES: protein motif:HMM:NF018647.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046556 - alpha-L-arabinofuranosidase
                     activity [Evidence IEA];
                     GO_process: GO:0046373 - L-arabinose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-arabinofuranosidase C-terminal
                     domain-containing protein"
                     /protein_id="HEY5051442.1"
                     /translation="MTNEPAATVTLDSAFVVGAIDRRLFGSFVEHMGRCLYSGIFEPD
                     HASADEWGFRRDVAELIGELGTSLVRYPGGNFVSGYDWRDGIGPVADRPTRLDLAWRS
                     IEPNLVGVDEFLPWARRLGLTPMMAVNLGTAGVADAAALVEYCNVAGGTYWSDARRKN
                     GTVDPYGVGLWCLGNEMDGEWQIGHKNADDYGKLANEAGKAMKLVDPTIELVACGSSN
                     HEMETFGRWESTVLEHTFEVADYISMHAYYQGAADRRDFLASGASMDRFIRDVLATAD
                     AVAARKKSRKRINISFDEWNVWSSEPDPVKPERAIQHAPRIAEDTYQPLDAVVVGDLF
                     STLLNHADRVTVACLSLLVNVSAPIITEPSGFAWRQSTFYPLAVAQRLATGNSLRSVV
                     SAPDHASPRYGDVPALSVAATLDEQQGRLSLFLVNRSATQLSVEVNTGDLEPIRVRRA
                     GQVTAASKWHPGNSDSLVEEISLERIAVEPRRLALAMRPESWVALDIEIAKRPALTVR
                     PTSRPGSASPVRPEAGRATSDHGGGRDQPAQ"
     gene            complement(2489..>3539)
                     /locus_tag="VII50_11120"
     CDS             complement(2489..>3539)
                     /locus_tag="VII50_11120"
                     /inference="COORDINATES: protein motif:HMM:NF018647.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046556 - alpha-L-arabinofuranosidase
                     activity [Evidence IEA];
                     GO_process: GO:0046373 - L-arabinose metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-L-arabinofuranosidase C-terminal
                     domain-containing protein"
                     /protein_id="HEY5051443.1"
                     /translation="NGSSNPYGIKLWCLGNEMDGPWQTGHKTAEAYGMLAAQSGRAMK
                     LVDPTIELVACGSSARDMPTFGSWETTVLGHTYDVVDYISLHAYYEEFDGDRRSFLGS
                     GAAMDGFIKDVVTTADAVGARLGSKKKIKLSFDEWNVWYQSRFPGAHDFPLEIASPRI
                     EDVYGTIDAVVVGDLLISLINNVDRVTVACQAQLVNVIAPIMTKPGGPSWRQTSFYPF
                     AETSRRARGTSLVAAIETASMATARYGEVPTLGVAASHDPDTSSVSIFLVNRSESAID
                     VDLRHEAFGALKVDDVYTIAADASGPLYGDMAAAAARPRLTSAWSTDPAGRTSLRLEP
                     ESWTTFSALTEGASR"
BASE COUNT          627 a         1123 c         1133 g          656 t
ORIGIN      
        1 cgccgagcgc catctgatcg ttggccacga atacggccgt gaccttgtct acgctcgcga
       61 gaatgcggcc agcttcgtag cctgattttg cgctccagtc gccgtgcaac aacggcggca
      121 cgtgagcagc cgcggatacc aaggcggccc tccagccggc tattctgccg tgcgtctcga
      181 gccagtcgtt cggaccagcg acgtgccaca cggtagcgtg gccagcggac agtagatgct
      241 cggtggcctg ccgcgcaccg tctacctggt cgacggaaac gactgggact cctcccagcg
      301 ggtcgccttc gacggccacg atgggaatgt ctacggacaa ctgaccgatc gcgccgagcg
      361 cggacacgag cggcacgtcg acaatgacgc cgtccacccc ctgagcgaca agtcggccga
      421 tggcctcgcg aactgatcca gggtcgatcg aactcaggct caccatcgtg acgaagtacc
      481 cttcagcacg cgcggcttgt tcgatgccat gcagcgtcga cgccggtccg aaaaggcggc
      541 tagcgaccgt gacgacgccc accgttcggg tgcgacccgt tgccaatgcg cgagcggcaa
      601 cgttgggcag atagccgagt tgctcgatcg cggcacgcac tcgcgcccgc gttgcgggcc
      661 gcacgcgatc gacctcgttc accactcttg agaccgtctg gtgggacacg ccggcgatcc
      721 tcgcgacatc ggccattgca ggccgcgatc cggcggcacg aaggccagct ggcagcgcca
      781 aattcccgag ccgacgctgc ttgctgccgg gcaccgccgg tggcggttgc tggtcggctt
      841 ggtgttgccg aacttggggc acccgcatct cctcattgag caggctgatc gcgcccaccg
      901 ccgtgatctg acgtggcacg gcctgcctcc ggacgtaccg gcgacgcgga gcctgggcgg
      961 cttgtcggac gtactgtcag cgcgggccgc ttcgcgattt cgatatcgag agcaacccac
     1021 gactcgggtc gcattgccaa ggcaagccgc cgcggctcga cggcgatccg ttcgagcgat
     1081 atctcctcga caagtgagtc actattgccg ggatgccact tgctggccgc ggtgacctgg
     1141 ccggctcgtc gaacacgtat cggttccaga tcgccggtat tgacctcgac ggatagctgc
     1201 gttgcggagc ggttgacgag aaatagcgat agccgaccct gttgttcgtc gagggtggcg
     1261 gccaccgaca gcgcagggac gtcgccgtac cgggggctcg catgatcagg tgccgatacg
     1321 accgaccgaa ggctgttacc cgtggccagc cgctgtgcga ccgccagcgg gtaaaacgtg
     1381 ctttgccgcc acgcaaaccc gcttggctcg gtgataatcg gcgcactaac gttcaccagc
     1441 agcgaaagac acgcgacggt gacgcggtcg gcatgattga gcaacgtgct gaaaaggtcg
     1501 ccgacaacta cggcgtcgag tggctggtag gtgtcctcag ctatccgcgg cgcgtgctgt
     1561 atggcccgtt ctggcttaac cgggtcaggt tccgaggacc agacgttcca ttcgtcgaac
     1621 gagatgttga tgcgcttccg gctcttctta cgcgcggcga ccgcgtcggc ggtagccagc
     1681 acgtcgcgga tgaagcgatc catcgaggcg cccgaggcca ggaagtctcg ccggtcggca
     1741 gctccctgat agtacgcgtg catggatatg taatcggcga cctcgaaagt gtgttcgaga
     1801 acggtggatt cccagcgccc aaatgtttcc atttcgtggt tggagctgcc gcaggccacc
     1861 agctcgatag tcggatcgac gagcttcatc gccttgccag cctcgttggc gagcttgccg
     1921 tagtcgtcgg cattcttatg accgatctgc cactcgccgt ccatttcatt gccgaggcac
     1981 cacagtccga ctccgtaggg atcgacggtg ccgttcttgc gtcgtgcgtc tgaccagtag
     2041 gtgccgccgg ccacgttgca gtattcgacc agcgcggcag cgtcggcgac ccctgccgtg
     2101 ccgagattga cggccatcat cggcgtcaga ccaagccgtc gcgcccatgg taggaactca
     2161 tcgacgccga ccaggttggg ctcgatcgat cgccacgcga gatccagccg agtgggtcga
     2221 tctgcaaccg ggccgatgcc gtcgcgccag tcgtagccag atacgaagtt gccgccggga
     2281 taacgcacga gcgacgttcc gagttcgcca atcaactcgg cgacgtcgcg tcgaaatccc
     2341 cactcgtctg ccgatgcatg gtccggttca aagatgccgg aatacaggca tcgccccatg
     2401 tgctcgacga aagatccgaa aagccgtcgg tcgatagcgc cgacgacgaa agcggagtcc
     2461 aacgtgacgg tggccgccgg ctcattcgtc atcttgatgc gccctctgtc aacgcgctga
     2521 atgtggtcca cgattccggc tccagccgca gggacgtccg gccggcgggg tcggtcgacc
     2581 acgcgctcgt gagccgtggt cgcgcggccg ccgcggccat gtcgccgtac agcggacctg
     2641 aggcgtccgc ggcgatggtg tagacgtcgt cgaccttcag cgcgccgaat gcctcgtgcc
     2701 gaagatcgac gtcgatcgcc gactccgatc tgtttaccag aaagatcgat acggacgagg
     2761 tgtccgggtc gtggctagcc gcgacaccca gtgtgggcac ctctccgtag cgcgcggttg
     2821 ccatcgaggc agtttcgatg gctgcgacca agctggtgcc ccgggcgcga cggcttgttt
     2881 cggcgaatgg gtagaagctc gtctgccgcc acgacggacc accgggtttc gtcatgatgg
     2941 gagctatgac gttgacgagc tgagcttggc aggcgacggt tacccggtcg acattgttaa
     3001 tcaggctgat caacaggtcg cccaccacaa ccgcgtcaat tgtgccgtac acgtcttcga
     3061 ttcggggcga ggcgatctcc agcgggaagt catgcgcgcc agggaatcgc gattggtacc
     3121 agacgttcca ttcgtcgaag gacagcttga tctttttctt gctgccgagc cgagcgccga
     3181 cggcatcggc cgtggtcacg acatctttga tgaagccgtc catggctgca ccggatccga
     3241 ggaagcttcg ccgatcaccg tcgaactcct cgtaataggc atgcagcgag atgtagtcga
     3301 cgacgtcgta cgtgtggccc aagacggttg tctcccagct accgaatgtt ggcatgtcgc
     3361 gcgccgagct accgcacgcg accagttcga tggtggggtc gaccagtttc atggctcggc
     3421 cagattgcgc cgcaagcatg ccgtaggcct cggcggtctt gtggccggtt tgccacgggc
     3481 cgtccatctc gttgccgagg caccagagtt tgatgccata ggggttagat gagccgttt
//
LOCUS       DATCBJ010000555         2797 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_530188, whole genome shotgun sequence.
ACCESSION   DATCBJ010000555 DATCBJ010000000
VERSION     DATCBJ010000555.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2797)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2797)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2797
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_530188"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(228..779)
                     /locus_tag="VII50_11125"
     CDS             complement(228..779)
                     /locus_tag="VII50_11125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009079374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEY5051444.1"
                     /translation="MPAKSKPSTSVVPSQRGAVKQPRWLSGGEQAMWRSFIESTQMLM
                     DRLDQELQRDSGMPHTYYEVLVRLSEVPDRRLRMSALADRSMSSRSRLSHAVARLEEN
                     GWVRRELCATDRRGQLAVLTDAGFAALAAAAPGHVEGVRTHLLDPLTPEQVEQLAQIC
                     AVIAERLRETGSSCFGDDENTDV"
     gene            884..1447
                     /locus_tag="VII50_11130"
     CDS             884..1447
                     /locus_tag="VII50_11130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HEY5051445.1"
                     /translation="MTATTTIETVPGYIAGTWAIDPVHSDVGFVVRHMVVAKVRGRFT
                     KFEGQIVTGENPADSSVTATIELSSIDTGNQQRDDHIRSADFFEVEKYPTMTYRSTGV
                     RQDGADLVLDGQLTLKGVTKDVPLRLELNGFGPDAYGGTRAGYTATADINRRDFGVNF
                     NAALETGGAVVADKIAIHLEVEAVLQP"
     gene            1580..>2797
                     /locus_tag="VII50_11135"
     CDS             1580..>2797
                     /locus_tag="VII50_11135"
                     /inference="COORDINATES: protein motif:HMM:NF013188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HEY5051446.1"
                     /translation="MTLLGLAGIIAISLIGPLLAVPRHYGVPIVVGELAVGVVLGTTG
                     LRVVHPNDPILSFLAQAGFGLVMLVAGSHVPIGDRALRKALRRGLILAVGTGIVAVPV
                     AAALSSMAHTHHTALYAVLLASSSAAVIMPIVQAEGLAGNRVLTLMAHAAIADTACII
                     ALPLAADPSKAGRAAVGAVIVLAVAGLAYLVLRELADRNLITELQNVSVRHSFGIEMR
                     VSLAALFALAGLAVNVHVSVMLAGFAFGLVLSAIGTPRRLASQLFAVTEGFLGPIFFI
                     WLGASIDLRALAGHPRLVVLALGLAVATTAIHALARLLGQPLPLAVLACAQLGVPVAA
                     VALGAHSDLLQPGEGGAILGAAILTLATAAVAGAYARRATGTATAATATAPAAVRTTA
                     GDDVGERTTPAAAP"
BASE COUNT          433 a          950 c          909 g          505 t
ORIGIN      
        1 gccgaggcga cagccgatgt cgctggtgcc gccggcgacg aaacagccgg cggtgtcgag
       61 gtcgctgaga tggtggctgg cgcgctgctg ccggccgaag ttgagtgcgt cttgctggaa
      121 cagccagcgg ccgcgatgag cgcgacgagc gtgatgccgc ccgctcgcgt cgtccatgaa
      181 gtagtcatgc gctcaaggac gcagaacggg ggccgttcgg gtgcatgtca aacatcggtg
      241 ttttcgtcgt caccaaaaca actggatccg gtttcgcgca gccgctcggc aatcaccgcg
      301 cagatctgcg ccaactgctc cacctgctcc ggtgtcaacg ggtccagcaa gtgggtccgc
      361 acgccttcga cgtggccggg agcggcagcc gcgagggctg caaatccggc gtcggtcagg
      421 accgcgagct ggccgcggcg gtcggtggcg cagagctcgc ggcggaccca accgttttct
      481 tcgagccgcg cgacggcgtg ggaaaggcga ctgcgcgacg acatcgaccg gtcggcgagg
      541 gcgctcatcc gcagtcggcg gtcggggacc tcggagagcc ggaccaggac ctcgtagtag
      601 gtgtggggca tcccgctgtc tcgctggagc tcctgatcca gccgatccat caacatctgg
      661 gtcgactcga tgaacgagcg ccacatcgct tgctctccgc cgctcagcca gcgcggctgc
      721 ttcacggctc cccgctgcga cggcacaacg gacgttgacg gcttcgactt ggcaggcatg
      781 ggggaatctt acataaaatt cgggaagata gttgaatctt caaccgttga atgctacgtt
      841 cgctctcaga ggttgagagc tcaataatcg aaaggtcgag acgatgactg ccaccaccac
      901 aatcgaaact gttcctggct acatagcggg cacctgggcc atcgacccgg tccactccga
      961 tgtcggcttc gttgtccggc acatggtcgt ggcgaaggtc cgtgggcgct tcaccaagtt
     1021 cgaaggccag atcgtgaccg gcgagaatcc ggccgactcc tccgtcactg cgacgatcga
     1081 gctctcctcg atcgacaccg gcaatcagca gcgtgatgac cacatccgca gcgctgactt
     1141 cttcgaggtc gagaagtacc cgaccatgac ctaccgctca accggcgtcc ggcaagacgg
     1201 cgccgacctg gtcctcgacg ggcagctcac gctcaagggc gtgaccaagg acgtgccact
     1261 gcgcctggag ttgaacggct tcggccccga cgcctacggc ggcacgcgtg ccggctacac
     1321 cgcgaccgcg gacatcaacc gccgcgattt cggcgtcaac ttcaacgccg cgctcgagac
     1381 cggtggcgct gtcgtcgctg acaagatcgc gatccacctc gaggttgagg ctgtactcca
     1441 gccgtaacac gcattcgacg tgatggccgg ccctacatca gggccggcca tcaccacgtt
     1501 gtgccactat cccgacgtaa tgaccggcac ggcatcaggg ccggccatca ccacgtcgcg
     1561 ggccagtatg ttgtctgccg tgactctgct cggccttgcc ggcatcatcg ccatcagtct
     1621 catcggtcca cttctggctg tgccgcggca ctacggcgtc ccgatcgtgg tgggcgagct
     1681 ggcggtcggt gtggtgctcg gcacgaccgg gctgcgggtg gtccatccaa acgatcccat
     1741 cctgagcttc ctcgcgcagg ccggtttcgg cttggtgatg ctcgtcgccg gttcccatgt
     1801 gccgatcggc gaccgcgccc tgcggaaggc cttacgtcgc ggcttgattc tcgccgtcgg
     1861 gaccgggatc gtcgcggtgc cggtggccgc cgcactctcc tcgatggcgc acactcacca
     1921 caccgcgctg tacgccgtgc tgctcgcgtc gagttcggcc gcggtcatca tgccgatcgt
     1981 gcaggctgag ggactggccg ggaatcgggt cctgaccctg atggcgcacg cggcaattgc
     2041 tgataccgcg tgcatcatcg cgttgccgct cgcagctgac ccgtcaaagg ccgggcgcgc
     2101 cgccgtcggg gctgtcatcg tgctcgcggt cgcgggcctc gcctatctgg ttttgcgtga
     2161 gcttgccgat cgcaacctca tcaccgagct gcagaacgtc agcgttcggc acagctttgg
     2221 catcgagatg cgggtcagcc tggcggcttt gtttgcgctg gccggactgg ccgtgaacgt
     2281 gcacgtgtcg gtaatgctcg ccggattcgc gttcggcttg gtgctttcgg caatcggcac
     2341 tccgcggcgg ctcgcgtcac agctgttcgc tgtcacggag ggcttcctcg gcccgatctt
     2401 cttcatctgg ctgggcgcgt cgatcgacct tcgagccctt gccggccacc cacggttggt
     2461 ggttctcgcc ctcggtctcg cggtcgcgac caccgcgatc cacgcactcg cccggctcct
     2521 cggtcagccg ttgccgctcg ccgtgctggc atgcgcgcaa ctcggcgttc ccgtcgccgc
     2581 ggtagcgctc ggcgcgcaca gcgacttgct gcagccaggc gaaggcggcg cgattctggg
     2641 agccgccata ttaaccctcg cgactgctgc ggtcgctggc gcttacgccc ggcgtgcgac
     2701 aggcaccgcg accgccgcga ccgctaccgc gcctgcagcc gtgcgcacga ctgcagggga
     2761 cgacgtcggc gagcggacca ccccggcggc cgccccg
//
LOCUS       DATCBJ010000556         2766 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_67793, whole genome shotgun sequence.
ACCESSION   DATCBJ010000556 DATCBJ010000000
VERSION     DATCBJ010000556.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2766)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2766)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2766
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_67793"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            440..880
                     /locus_tag="VII50_11140"
     CDS             440..880
                     /locus_tag="VII50_11140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5051447.1"
                     /translation="MNRLPSHTLNRSRRAARGLAAVAAVTLAGAVSAQAQVVFPAPGT
                     MQFSDLPSHVRAGATFTLHEVMPLAIFAGRMKFQRQLPSGWSTMASAPPRPKVFWLHW
                     KVPASWGGSQIAVRFVLESHGLTLAVSPTYAMSVTAPAKHRRHR"
     gene            877..1386
                     /locus_tag="VII50_11145"
     CDS             877..1386
                     /locus_tag="VII50_11145"
                     /inference="COORDINATES: protein motif:HMM:NF012573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5051448.1"
                     /translation="MIALRTLARRWRLVAVPVAAAVIGCSWTMASASGDVSHVGWPHR
                     DTVWFANNSGQVGVGTNGNDMLLGGAGSDTIYGGAGDDVIWGDRLPSPNGADQTDYLW
                     GGPGNDWIYTSHGTNHVDAGSGDDHVFAYFGNGTIDCGPGYDVLTLSKSDQHSYTVRN
                     CEVVTIGFP"
     gene            1739..2380
                     /locus_tag="VII50_11150"
     CDS             1739..2380
                     /locus_tag="VII50_11150"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025217.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEY5051449.1"
                     /translation="MHPQCHFGLDTLSWARRGACVTGLDFSAPAIEAARDLSQRSGLE
                     GEFVQANIYDAIEALGGRTFDVVYTGLGAINWLPDINGWAQTMIALTVPGGRFYLAEF
                     HPFTEVFGDEQLNVERSYFDRGPQIDETPGSYADLAVPTCHSRTVGWHHPIGDVVSAL
                     ADAGLRIDLLHEHDYTLFPRWPFLEHDEGQPYRLPAEVPALPLMYSLLGTRPQ"
     gene            complement(2391..>2766)
                     /locus_tag="VII50_11155"
     CDS             complement(2391..>2766)
                     /locus_tag="VII50_11155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5051450.1"
                     /translation="VVVPSMLFNFGGSICFLSMSVLLTSEMDSEHSGLGAGLFNAGRQ
                     VGGSIGLAALTVIAAAHTRSLLSAHHGAIAAADARGYGVALLTGAGLLGVGILVVAAH
                     GNRRRVRTAVGVDAESQPVGLA"
BASE COUNT          464 a          959 c          913 g          430 t
ORIGIN      
        1 ggcttggggt cgctcggcgg acaatcccgt cggcggctgg tacggattga agaagggtct
       61 tcgcggccgg tttggcaatt acgtcccgcc gatcctcgag gcgctcggtc tggccgaggt
      121 cgagcacaac ccgcgcaaca accggatgcg cgccatctga cgcacgcttc aggggcggcc
      181 gtcggcgggt gagcgagcga gcggctgctc gcacgcggct gcgcgagcgc gcacagcgga
      241 gggcaccgtc gatcgcagcg ccacgcgccg cgacagcccg gggctgcgac gccgatcagc
      301 gtttgcgtcg gccggacata cgtcctctct cggctacgga gatcgcgcaa accggccaat
      361 ttgccgagtg aatgaggagc gtgccctcgg tatctcgttc gcacctgcgg acgcctgcgc
      421 acggcgcagg accaacctca tgaatcggct tccatcgcac actctcaatc gctctcgccg
      481 cgcggcgcgc gggctggccg cggtcgcagc ggtgaccctc gccggagcgg tgtcagctca
      541 ggcccaggtc gtgtttccgg cccccgggac gatgcagttc agcgacctac cgtcacatgt
      601 gcgtgcgggt gcgaccttca ccctgcacga ggtgatgccg ctggcgatct tcgccggccg
      661 gatgaaattc cagcgccagt tgccgtccgg ctggtcaacg atggcctccg cgcctccacg
      721 cccgaaggtg ttctggctgc attggaaggt gccggcaagc tggggcgggt cgcagatcgc
      781 ggtgcggttt gtgctcgaga gccacggcct gacgctggcc gtcagcccga cctacgcgat
      841 gagcgtgacg gccccagcga aacaccggcg acaccgatga tcgccctgcg gacactcgcc
      901 cgccgctggc ggctggtcgc ggtcccggtc gcagccgccg tgatcggctg ctcgtggacc
      961 atggcgagcg ccagcggcga cgtcagccac gtcggctggc cgcaccgcga cacggtctgg
     1021 ttcgcgaaca acagcggcca ggtcggagtc ggcacgaacg gaaacgacat gctgttgggc
     1081 ggcgccggat cggacacgat ctacggcgga gcaggtgacg acgtcatctg gggtgatcgt
     1141 cttccgagcc cgaacggggc cgaccaaacg gactacctgt ggggcggccc gggtaacgac
     1201 tggatctaca cgagccacgg aacgaaccac gtggatgccg gcagcggcga cgaccacgtg
     1261 ttcgcctact tcggcaacgg cacgatcgac tgcggtcctg gctacgacgt cctgaccctc
     1321 tcgaaatccg accagcactc ctacacggtc cgcaactgcg aagtggtcac gatcggcttc
     1381 ccgtgacccg cgacgccgtc ggcatcgcgc ccctcggctt cgggtaaccc ccggggcctg
     1441 gacaggagat cgtcacgcgc acgtaacgcg ccatggcgag gtgtcgaggt cggcgcgcgc
     1501 gaggcctcct ttggtcgacc tcgcgatagc gacggaatca acgatccagg gatgcgacgc
     1561 gcgctagttt gtggtcaccg gtggccgagc gcttgcctct ctccgccctg ctctcccagg
     1621 cgctcgtcgc gttcacgatc gagctggaca acgcctggga gtccgcgatg cagcatcgga
     1681 cgacgcgcta cggcggctcg cgctcgacga gatcggcgat gtccgcggct gcacgctcgt
     1741 gcacccgcaa tgccacttcg gtctcgacac gctctcgtgg gcgcgccgcg gagcctgtgt
     1801 gacgggtctc gacttctccg cccccgccat cgaggccgcg cgagacctct cccaacgcag
     1861 cggccttgaa ggcgagttcg tacaggcgaa catatacgac gccatcgagg cgctcggcgg
     1921 tcgcacattc gacgtcgtct acaccggcct cggggcaatc aactggctac ccgatatcaa
     1981 tggctgggcg cagacgatga ttgcgctgac cgtgcctggt gggcggttct acctggcgga
     2041 gtttcatccc ttcaccgagg tctttggcga tgagcagctc aacgtcgagc gctcatactt
     2101 cgaccgaggc ccacagatcg acgagacgcc cgggagctac gctgaccttg cggtaccgac
     2161 atgccacagc cggaccgtcg ggtggcacca tccaatcggc gacgtcgtct cggcgctcgc
     2221 ggacgcagga ctgcggatcg acctgctgca cgagcacgac tacaccctgt ttccccgctg
     2281 gccctttctc gaacacgacg aagggcagcc gtaccgactg cccgcggaag tgccagcgct
     2341 cccgttgatg tactcgcttc tgggcacccg accccaatag acgccgggca tcatgccaac
     2401 ccgaccggtt ggctttcggc gtcgactccg acggctgtcc gcacgcgccg gcggtttccg
     2461 tgcgccgcga cgacaagtat cccgacgccc agcagaccgg cacccgtgag gagggccaca
     2521 ccgtacccgc gggcgtcggc cgcggcgata gcgccgtgat gagcgctgag cagcgaccgc
     2581 gtgtgagccg cggcgatgac cgtcaacgca gcgaggccaa tcgagccgcc gacctggcgg
     2641 ccggcgttga acaggcccgc gcccagaccg gaatgctcgc tgtccatctc ggaggtcaag
     2701 agcacgctca tcgagagaaa acagatcgat ccgccgaagt tgaacagcat cgacgggacc
     2761 acgacc
//
LOCUS       DATCBJ010000557         7038 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_11569, whole genome shotgun sequence.
ACCESSION   DATCBJ010000557 DATCBJ010000000
VERSION     DATCBJ010000557.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 7038)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7038)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7038
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_11569"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..213)
                     /locus_tag="VII50_11160"
     CDS             complement(<1..213)
                     /locus_tag="VII50_11160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5051451.1"
                     /translation="MTVLADEAGGYGRLERVSTSPSDEEFAIRARSFGAVARDYDRAR
                     PSYTAELVDDVVARIPGRDVVEVGAGT"
     gene            357..1541
                     /locus_tag="VII50_11165"
     CDS             357..1541
                     /locus_tag="VII50_11165"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY5051452.1"
                     /translation="MGTSSRRWLILGLGWAAQAAMCCYVFGLPMLVPAIRHTYGISLA
                     RSGVVVASPGFGVLFTLFAWGYAADRWGERLVITVGLAGASVFLTAACLVHGLIALCA
                     LLALAGAAGASVNAASGRVVLGWFDAHERGFAMGVRQTAQPLGVGLAALTLPALARHG
                     LSTAFVFPAALCAVVAVLVLALVTDPPRAAKASAAARSPYRTAQLWRVHGSSMLLVVP
                     QFAVSAFVLEYLVNQRNWGVLAAGRLVFAMQVAGAAGRVGAGAWSDRVGSRLRPMRQI
                     AVASALTMVAIAIGDARHAAFVVVAFAVGAVVTVTDNGLGFTAAAEIAGTAWAGRALG
                     AQNTAQNIAAILTPPLLGVLIESTSYSTGFLVASIFPVVAVALTPVPRRADVTFAALR
                     GQ"
     gene            1570..1962
                     /locus_tag="VII50_11170"
     CDS             1570..1962
                     /locus_tag="VII50_11170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009082411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEY5051453.1"
                     /translation="MTSTPVNLTAALASFDEVYSPRIVARVNDYDVRIAHARGEHVWH
                     VHEDTDEFFLVLDGQLEVAVRDAAGAETTVLLRQGDTFVVPRGTEHKPSSSGGSVLMF
                     EPGGTLTTGDRHEGEIPDHVDSTTGHDL"
     gene            2081..3964
                     /gene="typA"
                     /locus_tag="VII50_11175"
     CDS             2081..3964
                     /gene="typA"
                     /locus_tag="VII50_11175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009083070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HEY5051454.1"
                     /translation="MPMSTRDDLRNVAIIAHVDHGKTTLVDAMLWQSGAFSEHQAEND
                     SVRERVMDSMDLEREKGITILAKNTAVLHSGVTINIIDTPGHADFGGEVERGLEMVDA
                     VLLLVDASEGPLPQTRFVLRKALQKKLPVVLVINKVDRPDARIAEVVDETYELFIDLM
                     DDDIDHASILDFPIVYACARAGRASLTRPDDGTMPDSPDLEPLFATILKTVPPPSFDP
                     DVPLRAHVTNLDASQYLGRLALLRVHSGTMKRGQQVAWCKSDGTIEKVRLTELLGTSA
                     LERVPIETAGPGDIVAIAGIPEITIGETIADPDDPRPLPIITVDEPSLSMTVGINTSP
                     LAGESGKKLTARLVKNRLDAELVGNVSIRVLNTERPDTWEVQGRGELQLAILVEIMRR
                     EGFELTVGKPQVVTKRVNGKVHEPMEQLTIDAPSDYQGVLIQLLALRKGRLEQMVDHG
                     TGWIRMEYIVPARGLIGFRTEFLTETRGTGLLHHVHERYEPWHGDLRTRPTGSLVADR
                     RGPTTGFALSNLQERGTMFVGPQTEVYEGMIVGENARQDDMDVNPTKEKKLTNMRQSS
                     SDVLVPLIPHKVLSLEQALEFCREDECVEVTPANVRIRKVELSMSEREKYRGRRAKAA
                     RED"
     gene            complement(4079..4414)
                     /locus_tag="VII50_11180"
     CDS             complement(4079..4414)
                     /locus_tag="VII50_11180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5051455.1"
                     /translation="MVTWLTRSLLFIVGGAVLAFAVTTRTITYHSTKHGATLDVRAVG
                     VILLLVGVFDLLLNFGVSMYLRDTGQPLDPYVANPATAALGTPTTVTRQLYAGVRPAH
                     DTQPIPPQV"
     gene            complement(4463..6535)
                     /locus_tag="VII50_11185"
     CDS             complement(4463..6535)
                     /locus_tag="VII50_11185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009075705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HEY5051456.1"
                     /translation="MTDDSFLRLQARTARFTLGRPRGFSVSPDGARVLFLRSRGGTDR
                     ATCLWSYEVAAGTEVLLADSVELLAAGLDSLPDAERARRERAREQAAGIVAYSTDAAL
                     ARAAFALSGRLFVVDVSSGIARELAAGGLVFDPHLDPTGRTVAYASGGALRVVDVDGD
                     GSDRELITEPGVTWGLAEFVAAEEMDRQRGFWWAPDGQSILAARVDESDVQEWHIGDP
                     SNPARTPHAIRYPAAGTPNAVVWLSLLGLDGRRTELSWDADALPYVVNVRWRRGSPPL
                     LQLASRDQRTMRTVDVDVETGATILVREDTDPDWLELVPGVPDRLPDGRLVCTVDDGD
                     TRRLVIGDHVVSPVGLQVRAVLARTPDVLFSASTEPTEQHLWVYFSDGTCVPLTSEPG
                     VHTGTGASSVLVVSSATLSSDGAQTVVRDNGVDVGRIASFAEPAPFSPRVSISKQGRR
                     ELRTALVLPRDHVDGSGLLPVLMDPYGGPHAQRVLASRGAYLTPQWLADQGFAVVIVD
                     GRGSPGRGLAWERAVAGDLATVVLEDQVEALQSVAAAHRDLDLSRVAIRGWSFGGYLS
                     ALAVLRRPDVFHAAVAGAPVTDWSLYDTYYTERYLGVDTSAASYAVSSLLADAPTLAR
                     PLMIVHGLADDNVVAAHSLRLSAALLAAGRPHSVLPLSGVTHMASQEEVAANLIKLEV
                     QFIRSSIG"
     gene            6581..>7038
                     /gene="mshB"
                     /locus_tag="VII50_11190"
     CDS             6581..>7038
                     /gene="mshB"
                     /locus_tag="VII50_11190"
                     /EC_number="3.5.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010125 - mycothiol biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-1-D-myo-inositol-2-amino-2-deoxy-alpha-
                     D-glucopyranoside deacetylase"
                     /protein_id="HEY5051457.1"
                     /translation="MTDRSLLLVHAHPDDETIGTGATMAKYVADGANVTLVTCTLGEE
                     GEILVPELVGIAADKADQLGGWRIHELEQACKALGVRDHRFLGRAGGYRDSGMMDTPA
                     NQNPRCFWQADLEVATAQLVEVIREVRPQVVVTYDDFGGYGHPDHIQVHRV"
BASE COUNT         1225 a         2295 c         2352 g         1166 t
ORIGIN      
        1 ggtacccgcg cccacctcga cgacatcgcg tcctggtatc cgtgcgacga cgtcgtcaac
       61 caactcggct gtgtacgaag gacgggcccg gtcgtagtcg cgggccactg cgccgaagct
      121 gcgtgcgcgg atcgcaaact cttcgtcgga cggcgaggtg ctcacgcgct cgagtctgcc
      181 gtatccgcca gcctcgtcgg caagcacggt cacaccgctc gtgctccggg aatccgagga
      241 tcggaagcac ggattccata gggcgtccga actcacctct tgggggcgat tgagcactat
      301 cccagatagt gagatccctg tctcggtgaa cgagatgacc gcgttacgat ccccgcgtgg
      361 gaacgtcgag ccgccgctgg ttgatcctcg gcctgggctg ggcggcccag gcggcgatgt
      421 gctgctacgt cttcggactc cccatgctcg tcccggcgat ccggcacacc tacggcattt
      481 cgttggctcg gtccggcgtc gtcgtcgcgt cgcccgggtt cggcgtcctg ttcacgctat
      541 ttgcttgggg ctacgcggct gaccggtggg gtgaacggct cgtcatcacg gtcgggctcg
      601 cgggtgcgtc ggtctttctc accgcggcct gcctcgttca tggattgatt gcgttgtgtg
      661 cattgctggc actcgccggt gcagcagggg cgtcggtgaa cgccgcgagt gggcgtgtcg
      721 tgctcgggtg gttcgatgcg cacgagcgcg gctttgcgat gggcgtacga caaacagcac
      781 agccgctcgg agtcgggctc gccgcactga ccttgcccgc actcgcccgg catggattgt
      841 cgactgcctt tgtctttcca gcagcgctct gtgccgtggt ggcggtcctg gtactcgcgc
      901 tcgtcaccga tccaccgcgt gcggcgaagg cttcggcagc cgcccggtcg ccctaccgca
      961 cggcacaatt gtggcgagtg cacgggtcga gcatgttgct tgtcgttccg cagtttgcag
     1021 tctcggcgtt cgtcctggag tacctcgtga accagcggaa ttggggtgtg ctcgcggcag
     1081 gtcgcctagt gttcgcgatg caggtggccg gcgccgccgg tcgagtcggt gcgggtgcgt
     1141 ggtcggaccg ggtcggcagc cgcttgcgcc cgatgcggca gatcgccgtc gccagcgcgt
     1201 tgacgatggt ggctatcgcg attggagatg cgcgccacgc cgccttcgtc gtcgtcgcgt
     1261 ttgcggtcgg cgcagtcgtc acggtgaccg acaacggtct cggattcacc gcagccgcgg
     1321 agattgccgg aacggcctgg gcggggcgcg cgctcggcgc gcagaacacc gcgcagaaca
     1381 ttgccgcgat cctcacaccc ccgctgctcg gcgtcctgat tgaaagcacg tcttacagca
     1441 cgggcttctt ggtagcgtcg atctttccgg tggttgcggt cgcactcacg cccgttccgc
     1501 gtcgcgcaga cgtgacgttt gctgccctca gggggcagta ggcgcctggt tgtggtacca
     1561 ctggcgacca tgacctccac tcccgtgaac ctgaccgctg cattggcttc gttcgacgag
     1621 gtgtacagcc cgcggattgt cgcgcgggtg aacgactacg acgtgcggat cgcccatgcg
     1681 aggggcgagc acgtgtggca tgtgcacgag gacaccgacg agttcttcct tgtcctcgac
     1741 gggcagttgg aggtcgcggt tcgtgacgcc gccggggccg agaccaccgt gctgttgcgg
     1801 cagggcgaca cgttcgtcgt accgagaggc accgagcaca agccatcctc gtccggcggt
     1861 tcagtcctca tgttcgagcc aggcgggaca ttgaccaccg gtgaccggca cgagggtgag
     1921 attcccgacc acgtcgacag cacgacgggc cacgatctgt agcccacgcg cgtgcccact
     1981 tcagggtcat gacggccggt aagcgtacga ccacgtacga tggggtaatc ccaacgagac
     2041 atgcccttgg gagccccacc ttggtctgga gcactttctc atgcccatga gtacccgcga
     2101 cgatctgcgg aacgtcgcga tcatcgccca cgtcgaccac ggcaagacca cgctggtcga
     2161 cgccatgctc tggcaatccg gtgcattctc cgagcatcag gccgagaacg attcggtccg
     2221 cgagcgggtc atggactcca tggacctcga gcgcgagaag ggcatcacga tcctcgcgaa
     2281 gaacaccgcg gtgctgcaca gcggtgtcac gatcaacatc atcgacacac ctgggcacgc
     2341 cgattttggc ggtgaggtcg agcgcggcct tgagatggtc gacgcggtgc tgctgctcgt
     2401 cgacgcctcc gaaggcccgc tgccgcagac gcggttcgtg ctgcgcaagg cgttgcagaa
     2461 gaagctgcca gtggtacttg tcatcaacaa ggtcgaccgg cccgacgcgc ggatcgccga
     2521 agtcgtcgac gagacctatg agttgttcat cgatctgatg gacgacgaca tcgaccacgc
     2581 gagcattctc gacttcccga tcgtctatgc gtgtgcgcgc gccggtaggg catcactaac
     2641 caggccggac gacggcacga tgcccgacag ccctgacctg gagccattgt tcgcgacgat
     2701 tctcaagacc gtgccgccgc cgtcgttcga ccctgatgtg ccgttgcgcg ctcacgtcac
     2761 gaacctcgac gcgtcgcagt acctcggccg gctcgcgctg ctgcgggtgc actccggaac
     2821 gatgaagcgc gggcagcagg ttgcctggtg caagtccgac ggcacgatcg agaaggtgcg
     2881 actcaccgag ctgctcggca ccagcgccct tgagcgggtg ccgatcgaga ccgctggccc
     2941 cggcgacatc gtggctattg cgggcatccc cgagatcacc atcggtgaga ccatcgccga
     3001 ccctgacgat ccgcgcccgc tgccgatcat cacggtcgac gagccgagcc tgtcgatgac
     3061 ggtgggcatc aacacctcgc cgctcgctgg cgagtcgggc aagaagctca cggcacggct
     3121 ggtcaagaac cggctcgatg ccgagctcgt cggcaacgtc tcgatccgcg ttctgaacac
     3181 cgaacgccca gacacgtggg aagtgcaggg tcgtggtgag ctgcaactcg cgatcctcgt
     3241 cgagatcatg cgacgtgaag gattcgagct cacggtgggc aagcctcagg tcgtcaccaa
     3301 gcgcgtcaac ggcaaggtgc acgagccgat ggagcaactg accatcgatg cgccgagcga
     3361 ctatcagggc gtgctcatcc agttgctcgc cctgcgcaag ggacgccttg agcagatggt
     3421 cgaccacggc actggctgga tccggatgga atacatcgtg ccggcgcgcg ggctgatcgg
     3481 attccgcacc gagtttctga cagagacgcg cgggacaggc ctgctgcacc acgtgcatga
     3541 acggtatgag ccatggcatg gtgatttgcg cacgcggccg accggctcgc tggttgctga
     3601 ccgccgcggc ccgacgaccg gcttcgcgct ctcgaacttg caagagcgcg gcaccatgtt
     3661 cgtcggcccg cagaccgagg tctacgaggg catgatcgtc ggtgagaacg cgcgccaaga
     3721 cgacatggac gtgaacccca ccaaggagaa gaagctcacc aacatgcgtc agtcgtcgag
     3781 cgacgtcttg gtgccgttga tcccgcacaa ggtgctgtca cttgagcagg cgctggagtt
     3841 ctgtcgcgaa gacgagtgcg tcgaagttac gccggcgaat gtgcgcatcc gcaaggttga
     3901 gctgtcgatg tccgagcgag agaaatatcg cggacgccgg gcaaaggccg cgcgcgagga
     3961 ctagttggcc tgcctgcgcg ccgagtggcg gcaaatgcac ctcactcagc ggcgtgtcgc
     4021 gtgtgtgaag cgcatatgtg cccactcggc ggcgcgggat caggtgcggg atcaagggtc
     4081 agacttgcgg cgggatgggc tgcgtgtcgt gggccggccg tacccccgcg tacagctgcc
     4141 gggtgactgt cgtaggggtg cccaacgccg ccgtcgcggg gttggcgacg tagggatcca
     4201 gcggctggcc ggtgtcgcga agatacatcg acacgccgaa gttgagcagc agatcgaaga
     4261 cgccgacgag cagcagaatg acgccgacgg cgcgaacgtc cagcgtcgcg ccgtgtttcg
     4321 tgctgtggta ggtgatcgtc cgggtggtga ccgcaaacgc cagcacggcg cccccgacga
     4381 tgaagaggag actgcgcgta agccaagtaa ccatgtgacg tgtctaccca tcagaacgcc
     4441 gatattcacc gcctgacggg gattatccga tcgaactgcg gatgaactgc acctcgagct
     4501 tgatcagatt cgcggcaacc tcctcttgtg acgccatgtg ggtcacgccc gacagcggta
     4561 gcacgctgtg cggtcgcccg gcagcgagca acgcggcgga cagtcgcagg ctgtgggccg
     4621 caaccacgtt gtcatcggcc agaccgtgca cgatcatcag tggccgagca agcgttggag
     4681 catcggccag cagtgaactg accgcatacg acgccgccga cgtgtcaacc ccgaggtacc
     4741 gctcggtgta gtacgtgtcg tacaagctcc agtcggtaac gggcgcaccg gcgacggccg
     4801 catggaagac atcgggacga cggagcacgg ccaatgccga aaggtagcct ccgaacgacc
     4861 aaccgcggat cgcgaccctt gataggtcca agtcgcgatg cgccgccgcc accgactgca
     4921 acgcctcgac ctgatcctcg agtaccaccg ttgcgagatc gccggccact gcccgctccc
     4981 aagccaagcc ccgaccggga ctgccgcggc cgtcgacgat caccaccgca aatccctggt
     5041 ccgcaagcca ttgcggggtc agatatgccc cccgtgacgc gagcactcgt tgcgcgtgcg
     5101 gaccgccgta agggtccatc agcaccggca gcaggccgga tccgtcgacg tggtcgcgcg
     5161 gcaacacgag ggcggtgcgc agctcgcgac gtccctgctt cgagatcgag acgcgaggcg
     5221 agaacggcgc cggctccgcg aacgatgcga tgcgtccgac gtcgacaccg ttatcgcgga
     5281 cgacggtttg cgcaccgtcg ctggaaagcg tcgcgctgct cacgacgagc acagaactcg
     5341 cgccggtgcc ggtgtgcacg ccgggttcgg acgtgagcgg tacgcacgta ccgtcggaga
     5401 agtagaccca caggtgctgc tccgtcggct cggtcgacgc gctgaatagg acgtctggag
     5461 ttcgcgcgag aacggcccgc acctgcagcc cgaccggcga aaccacatgg tcgccgatga
     5521 cgagtcgccg agtgtcgccg tcgtcgaccg tgcacacgag ccggccgtcg ggcagccgat
     5581 cgggcacgcc gggcaccagc tcgagccaat caggatcggt gtcttcgcga accaggatcg
     5641 ttgcacccgt ctcgacgtcg acgtcgacgg tgcgcatcgt gcgctggtcg cgcgacgcga
     5701 gctggagcaa cggcgggctg ccgcgacgcc accgcacatt caccacgtac gggagggcgt
     5761 cggcgtccca ggaaagctcc gtgcggcgac catcgagccc aagtaacgac aaccacacca
     5821 ctgcattcgg ggtaccggcg gccgggtagc ggatcgcatg cggtgtccga gcgggatttg
     5881 atggatcgcc tatgtgccat tcctggacat ccgattcatc gacgcgcgcc gcgagtatcg
     5941 actgaccgtc cggtgcccac caaaaccctc gctgccggtc catctcctct gccgcgacga
     6001 actcggcgag cccccatgtc acgcccggct cggtgatgag ttcacgatcc gacccgtcac
     6061 cgtcaacgtc gaccacacgt aacgcgccgc cggacgcgta ggccaccgtg cgtccggtcg
     6121 gatcgaggtg cggatcgaag accaggccgc ccgcggcgag ttcacgggca atcccgctcg
     6181 agacgtcgac aacgaagagg cgcccagaca gcgcgaacgc ggctcgggcg agggcggcgt
     6241 cggtggagta agcaacgatg ccggccgctt gctcgcgagc cctctcgcgg cgggcgcgtt
     6301 cggcgtccgg gagcgagtcg agccccgcag ccagcagttc gaccgaatcg gccaacagga
     6361 cctcggtgcc ggccgctacc tcgtagctcc acaggcaggt tgctctatcg gtgccgccgc
     6421 gcgagcgcag gaacagcacc cgtgcgccgt cgggggagac gctgaacccg cgcggccggc
     6481 cgagggtgaa ccgcgcggtg cgcgcctgca gtcgcaggaa ggagtcgtcc gtcaccggcc
     6541 cgatcctgcc atgtcggcct cgccctttag ggttgcctgc atgaccgatc gcagcctgtt
     6601 gctcgtgcac gcccatccgg acgacgagac gatcggcacc ggcgccacta tggccaagta
     6661 cgtggccgat ggcgcgaacg tcaccctcgt cacgtgcacg ctgggtgaag agggcgaaat
     6721 cttggtacct gaactcgtgg gtatcgcggc cgacaaggcc gaccagctcg gcggctggcg
     6781 aatccacgaa ctcgagcagg cgtgcaaggc actgggcgtc cgagatcatc gatttctcgg
     6841 tcgggcaggc ggttaccgcg actcagggat gatggacacg ccggcgaacc agaatccacg
     6901 ttgcttctgg caggccgacc tggaggtcgc gacggcgcaa ctcgtcgagg tgatccgcga
     6961 ggtccgcccg caggtggtcg tgacgtacga cgacttcggt ggctacgggc accccgacca
     7021 catccaggtg caccgggt
//
LOCUS       DATCBJ010000558         2137 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_88436, whole genome shotgun sequence.
ACCESSION   DATCBJ010000558 DATCBJ010000000
VERSION     DATCBJ010000558.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2137)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2137)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2137
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_88436"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1190
                     /locus_tag="VII50_11195"
     CDS             <1..1190
                     /locus_tag="VII50_11195"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY5051458.1"
                     /translation="VFDPKERAKAIGIWAGAVGMGIAIGPIVGGALLGHFWWGSVFLI
                     NVPIVIIGAILIRVLVPESRNPNPGRHDPLGVVLEVVGLVLVIYAIIKAGDLGSLRPV
                     QVWAPFVVGAVALAVFVWYERRSDHPTLDMALFRDPRFSAAVGSVGLVFFALMGTTFF
                     MVFYLQIARGYSPLQAGVRFLPLAFAQIIASPRSPKLVRRYGAKAVCTSGLLLMTATF
                     VGYVTLGQHTSIWVLEGLFFLQGLAMGNIMPPATESIMSSVPREKAGAGSAINNVARQ
                     VGAALGIAVLGSVLASSYRKGIDPTLTKLPITAAVRGQMEKSVEATQAVVEKVGASLQ
                     SVIPASNTAFIHAMHVTSLFSAIFGLAGTFVAFKFLPGRRPADVGAPATDAPVQESVM
                     VAE"
     gene            1187..1804
                     /locus_tag="VII50_11200"
     CDS             1187..1804
                     /locus_tag="VII50_11200"
                     /inference="COORDINATES: protein motif:HMM:NF028169.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEY5051459.1"
                     /translation="MTTIGRPRSVDADRAIIDAVLEEVIAVGIDGMSIEQIAARAGVA
                     KATVYRRWPNKEALLLDAVTGVQVEVPTLAGISVRDDLVTLVDSMRRRMADSAQPGES
                     LASRLYPCMIAEGGRHPEIAAKYKQRVVEQRREAVRVVIRRGIADGDLRADLDVETML
                     LLLVAPMLVQLYMWSAAVELPPESSATYVDGVLDGLGLHQDRRVG"
     gene            complement(1772..>2137)
                     /locus_tag="VII50_11205"
     CDS             complement(1772..>2137)
                     /locus_tag="VII50_11205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD+ synthase"
                     /protein_id="HEY5051460.1"
                     /translation="QVTTGDEPPIPQNSIDKPPSAELRPDQLDSDSLPDYDVLDRVLE
                     GYIEQDLGRDALVAAGHDPVVVDRVIRLVDGSEYKRRQYPPGPKISGRNFGRDRRLPI
                     TNRWREPSALPDAPVLVQP"
BASE COUNT          327 a          662 c          726 g          422 t
ORIGIN      
        1 acgtcttcga cccgaaggag cgcgcgaagg ccatcggtat ttgggccggc gccgtcggca
       61 tgggcatcgc gatcggcccg atcgtcggag gcgcgctgct gggtcacttc tggtggggca
      121 gcgttttcct gatcaacgtg cccatcgtca tcatcggggc gatcctcatc cgtgttctgg
      181 tgccggaatc ccgcaacccc aaccccggtc ggcacgatcc gctcggtgtc gttctcgaag
      241 tcgtcggcct cgtactggtc atctacgcga tcatcaaggc gggcgacctc ggcagcctgc
      301 gtcctgtgca agtctgggca ccgttcgtcg tgggtgcggt cgccctcgct gtgttcgtct
      361 ggtacgagcg gcgcagcgac catccgacac tcgacatggc gctgttcagg gacccgcggt
      421 tctcggccgc agtcggttcg gtcggcctgg tgttctttgc gctgatgggc accacgttct
      481 tcatggtgtt ctatctgcag atcgcacgcg gatactcacc gctgcaggct ggcgtccgct
      541 tcctgccgct cgccttcgcg cagatcatcg catcgccacg cagccccaag ctggtgcgca
      601 ggtacggcgc gaaggcggtg tgcacgagcg gtttgctttt gatgacggcg acatttgtcg
      661 gctacgtcac cctgggccag cacacgtcga tctgggtact cgagggtctc tttttccttc
      721 agggcttggc aatgggcaac atcatgcctc cggcgactga atcgatcatg tcgtccgtgc
      781 ctcgcgaaaa ggccggtgcg gggtccgcga tcaacaacgt cgcaaggcag gtcggtgcgg
      841 cgctcggtat cgccgtcctc ggttcggtgc ttgcatcgtc ctaccgcaag ggcatcgatc
      901 cgacgctgac gaagcttccg atcaccgccg cagtgcgcgg tcagatggag aagtcggtcg
      961 aagctacaca ggccgtcgtt gagaaggtcg gcgcgtcgtt gcagtcggtg attccggcgt
     1021 cgaacaccgc ctttatccac gccatgcacg tgacgtcgct gttctccgcc attttcggtc
     1081 tggcgggcac gttcgtggca ttcaagttcc tgccaggacg ccgaccagct gacgtcggtg
     1141 caccggctac cgacgcgccg gtgcaagaaa gcgtcatggt cgccgaatga ccacgatcgg
     1201 ccggccgcgg agcgtagacg ccgaccgcgc gatcatcgat gcggtcctcg aggaggtcat
     1261 cgctgtcggc atcgacggca tgtcgatcga acagattgcg gcgcgcgcgg gcgtcgcgaa
     1321 ggcgacggtg tatcggcgtt ggccgaacaa ggaagctctg ctgctcgacg ctgtcaccgg
     1381 ggtgcaggtc gaggtgccta cactcgccgg catctcggtg cgtgacgacc tcgtcacgct
     1441 ggtcgacagt atgcgtcgtc ggatggctga ttctgcccaa ccgggcgaat cgcttgcgtc
     1501 gcggctgtac ccgtgcatga ttgccgaggg cggccggcac ccagagatcg cagcgaagta
     1561 caaacagcgc gtagtcgagc aacgccgaga agcggtgcgc gtggtgatcc ggcgcgggat
     1621 cgcggacggc gacttgcggg ccgacctcga tgtcgagacg atgctgctcc tgctcgtcgc
     1681 cccgatgctt gtgcagctgt acatgtggtc ggcggcggtc gagttgccgc cggaatcttc
     1741 ggcgacatac gtcgacggtg tgctcgacgg gctagggctg caccaagacc ggcgcgtcgg
     1801 gtaacgcgga cggttcgcgc cagcggttcg tgatcggcag gcggcggtcg cgaccgaagt
     1861 tgcgtccgct gatcttcggt ccgggcgggt actggcggcg cttgtactcc gagccgtcga
     1921 cgagccggat cacccgatcg actacgaccg gatcgtggcc ggcagcaaca agtgcgtcgc
     1981 gaccaaggtc ctgctcgata tagccctcga ggacgcggtc gagtacgtcg tagtcgggca
     2041 gcgaatcgct gtcgagttgg tcgggacgca gttcggccga tggtggcttg tcgatcgagt
     2101 tctgcgggat cggtggctcg tcgccagtgg tcacctg
//
LOCUS       DATCBJ010000559         5347 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_162757, whole genome shotgun sequence.
ACCESSION   DATCBJ010000559 DATCBJ010000000
VERSION     DATCBJ010000559.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 5347)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5347)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5347
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_162757"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..639)
                     /locus_tag="VII50_11210"
     CDS             complement(<1..639)
                     /locus_tag="VII50_11210"
                     /inference="COORDINATES: protein motif:HMM:NF016101.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HEY5051461.1"
                     /translation="MPSSAVLTGHRVIVFIQENKTTEFYFPTMKAWGADVADNGSLLD
                     APPNFDQPHDRDAWVHYAMGDYPALVAQIDNDTVIPFYSWLAKQFVFADHHFGLGSNS
                     TSGHMLAVGGQTPTMKNPPFVGAHPVWNIPSIFSVAQSAGVSWAAFPDATGYPTKFYT
                     SLNIAPGTDNIYRPAEFIPMAKAGTLPQICYVWSPAGYDEHPPATSNPGYVTN"
     gene            1202..1438
                     /locus_tag="VII50_11215"
     CDS             1202..1438
                     /locus_tag="VII50_11215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5051462.1"
                     /translation="MSKVMVSMADELLAEIDAEAKRRATSRSALLAVAARRELDRRDP
                     VQVAEAIARSEQRFREAGSFDAAELVRGDRDSQR"
     gene            1435..1872
                     /locus_tag="VII50_11220"
     CDS             1435..1872
                     /locus_tag="VII50_11220"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HEY5051463.1"
                     /translation="MTRLLVDTSVLIKWFHSQGETEVPQARALRTAHTNGQLDAHVLD
                     LAYYELGNVLIRALGWTADDVADQLDDLATILGPPLVVTAPRLRQAAELAHAHALSFY
                     DAVWAAAAQQLAIPLVSADRLLLAAGLAQTPTDIATRLTLPTI"
     gene            2161..3177
                     /locus_tag="VII50_11225"
     CDS             2161..3177
                     /locus_tag="VII50_11225"
                     /inference="COORDINATES: protein motif:HMM:NF012913.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HEY5051464.1"
                     /translation="MLTRVAVFALGGTIASSADPAGAAIVGLNGVDLLNTVPGVIAVA
                     DVEVFTVAMVPSGDLRLRHLFELRDQVAAAILAGTQGIVVTQGTDTLEETAYAFELLT
                     SFDEPVVFTAAMRSSDALSADGPANLMAAVRVAASPDARACGVLVVLNDEIHGATDVR
                     KMHTSSTAAFASPLVGPVGYVTEGRVRVHRKPAGRVTIDVPRDAEDARVALVTVGFDD
                     DDGLIGAVTGLGYDGLVLAAFGGGHVPSWTVPIIERVAKRIPTILASRTAGGEVLRHS
                     YGYAGSETDLLARGLISAVGLSAAKARILLRLLLVAGVDRDQVARCFEEAAEPAASDR
                     RYPA"
     gene            complement(3205..3528)
                     /locus_tag="VII50_11230"
     CDS             complement(3205..3528)
                     /locus_tag="VII50_11230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5051465.1"
                     /translation="MAETQSSAQPNPSAPFNAWQRATAAFREAEHRGAAHADIVRLAE
                     QVIRARNALVLDRLEAGWQPRDDVLHHLICDEDLLHEKDDSGYHDIAASTTQARPAVV
                     PAPPS"
     gene            3695..3928
                     /locus_tag="VII50_11235"
     CDS             3695..3928
                     /locus_tag="VII50_11235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5051466.1"
                     /translation="MSESARSGRPDGVFEPGAIEVLERWQNFGATWRVVSRIEGSVTL
                     SLCRCDGGEEVQRLTSSDVDLLGWLAGRTSSEQ"
     gene            4011..5249
                     /locus_tag="VII50_11240"
     CDS             4011..5249
                     /locus_tag="VII50_11240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY5051467.1"
                     /translation="MASSDTFVIVGAGLAGAKAAEALRSQGFDGRLILLGAEHHTPYE
                     RPPLSKDYLTGKAPREKVFVHAHRWYADHDVELRSGQFVTDLDRATRGVGLADGSVVS
                     YDKLLLTTGASPRELGVPGADARGVHYLRTLDDSDVLRGTLATASRLVVIGAGWIGLE
                     VTAAARLVGVEVTVVETAALPLLRVLGPEVAQVFANLHREHGVEFRFDASLAEIVTDN
                     GQATGVRLNDGTTIEADAVLVAVGATPNVQLGEAAGLRVDNGIVVDAALRTSDPNIFA
                     AGDVANAFHPMYNRHIRVEHWANARQQPKAAATSMLGNDASYDELPYFYTDQYDLGME
                     YVGFVEPDGYDQVVFRGDVARREFIAFWLSNNRVVAGMNVNVWDVVEPIKALIRSRVA
                     IPAGRLADRGAALMDLVQPS"
BASE COUNT          890 a         1642 c         1812 g         1003 t
ORIGIN      
        1 gttggtgacg taaccggggt tgctggtggc cggtgggtgt tcgtcgtagc cggcggggct
       61 ccacacgtag cagatctggg gcagcgttcc ggctttcgcc atcgggatga actcggccgg
      121 gcggtagata ttgtcggtgc caggggcgat gttcaaactg gtgtagaact tcgtcgggta
      181 gccggtggcg tcgggaaacg ccgcccacga cacgccggct gactgggcga cgctgaagat
      241 cgacgggatg ttccagaccg ggtgtgctcc gacgaacgga gggtttttca tcgtcggggt
      301 ctgcccgcca acagccaaca tgtgcccgga ggtggagttc gacccgagcc cgaagtggtg
      361 atcggcgaac acgaactgct tagccaacca gctgtagaac gggatgacgg tgtcgttgtc
      421 gatctgggcc accagcgccg ggtagtcacc catcgcgtaa tgcacccacg cgtcacgatc
      481 gtgcggctgg tcgaagttcg gtggcgcgtc cagcagcgag ccgttatcgg cgacatcagc
      541 gccccacgcc ttcatcgtcg ggaagtagaa ctcggtggtc ttgttctcct ggatgaacac
      601 gatcactcgg tgaccggtca acacggccga actcggcatc gcggcgctcc gctctactca
      661 gcccccgcgg tgcgccttcg cgcgccgtct cctcaagaca accacgtccc atgccacggt
      721 tagatcgtcg ctgcgatcac gctcgatgct ggcccactaa cgaacgctcc gccgatcacg
      781 gcgctttgcc cctcgtgcgc ggcccagccg atgccgtcga tggcttgaac cacgtcatga
      841 gggccgttgc ccaggcgtgg caggcggccg agtgggcgaa gatggttggg ccggcgggat
      901 cgaacccgtt ggccggtaac ggcggagcat cgaaacaacg gccacgatgt cggccggcgc
      961 ccacctgtga tcacaatctc aagtctgccg gtacgcaaca ccgatccagt gattccggct
     1021 acaccggcga cgaactagtc cccgcgtaga tcgctcgatc ttcgtccacg atgcctgaat
     1081 tggacggacc caactccgcg aagaagtgcc ccggaactac cggatctcga cagatcgtgt
     1141 tgggagggca tgctttcgcg gtcgcctcgc tccgggcagt accggtggct atactattgg
     1201 tatgtctaag gtgatggtgt cgatggcgga tgagctgctg gccgagatcg atgcggaagc
     1261 caagcggcgg gcgacgagtc gtagcgcgtt gcttgcggtg gcggcgcgcc gggaacttga
     1321 tcgccgtgat ccggtgcagg tcgcggaggc gatcgctcgg tcggagcaac gcttccgaga
     1381 ggcgggcagt ttcgacgcgg ctgagctggt tcgtggtgat cgcgactccc agcggtgacc
     1441 cgactgcttg ttgacacgtc ggtgctgatc aagtggttcc acagtcaggg cgaaactgag
     1501 gtgccgcaag cccgtgcgct gcgcaccgcc cacacgaatg ggcagctgga tgctcatgtg
     1561 ctggacttgg cgtactacga gttgggcaac gtgttgatcc gcgccttggg ctggactgca
     1621 gacgacgtcg cggatcagct tgatgacctg gccaccatcc tcgggccacc gctggtggtg
     1681 accgctccac ggttgcggca agcggcggaa ctggcccacg cccacgcgct gtccttttac
     1741 gacgcggtct gggcggccgc tgcgcaacaa ctcgcgatcc cactcgtcag cgcggaccgt
     1801 ttgctgctgg cggcggggct ggctcaaacg ccgaccgaca tcgctacgcg actgacgctg
     1861 cccaccatct gacaccggcg tctgcgccgc ggtgtcgtat cacgacgacg tcgcgttggt
     1921 gtgctgaagt tgatcactgc cggaaacaga tgggcaacac gacagtcaaa accagccagc
     1981 gctagtcaac gatgaacacg tgaattacat tgtgaggtaa gggttttcaa cggtagccaa
     2041 cgacgttcag ccgctgcgag atggcactct ccaggcggta gcgcgggttc gaatcccgtc
     2101 gcggctacac acaaaaccgc aggtcagggc gcctgcgcct tgcggatagg cggccgtctt
     2161 gtgctgacca gggttgccgt cttcgcgctt gggggaacga tcgcgagctc cgccgaccct
     2221 gctggcgccg cgatcgtggg cctcaacgga gtcgacttgc ttaacaccgt gcccggagtc
     2281 atcgcggtcg ccgatgtcga ggtcttcacc gtggctatgg tgccctcggg cgatctgcgc
     2341 cttcgccatt tgttcgaact ccgcgatcag gttgctgcag cgatcctcgc tgggacgcag
     2401 ggcattgtcg ttacgcaggg cacggacacg ctcgaggaaa ccgcttatgc gtttgagcta
     2461 ttgacgtcgt tcgacgagcc ggtggtcttc accgcagcga tgcgcagttc ggatgcgctc
     2521 agcgcagacg gtccggcgaa cctcatggca gcggtacgcg tggctgctag tcctgacgcc
     2581 cgcgcctgtg gtgtgctcgt tgtcctgaac gacgagattc acggtgctac tgacgtgcgc
     2641 aagatgcaca ccagctcgac tgcggcattt gcgtcgccgc tcgtcggacc ggttggttat
     2701 gtcaccgagg gccgggtgcg cgtgcacagg aagcctgcgg ggcgggtcac gatcgacgtg
     2761 ccacgggacg ccgaggatgc ccgggtcgca ctagtgacag tcggtttcga cgacgacgac
     2821 gggcttatcg gtgcagtaac cggtctcggg tatgacgggc tcgtccttgc cgcgttcggt
     2881 ggcgggcacg tgccgagctg gacggtccca atcatcgagc gtgtggcaaa acggatcccg
     2941 acgattcttg cttcgcgcac ggcagggggt gaggtgttgc gccacagcta tgggtacgcc
     3001 ggctcagaaa ccgatttgct cgcgcgtggc ttgatatcgg cggtcgggct cagtgccgcg
     3061 aaagcgcgga tcttgcttcg gctgttgctt gtcgcgggcg tagatcgcga tcaagttgcg
     3121 cgttgctttg aggaggcggc ggaaccggcc gcctccgatc ggaggtaccc ggcgtgaacg
     3181 cgagcccggc tgcgttcgac gcggctacga cggaggggcg ggtactactg ccggacgcgc
     3241 ctgcgtggtg ctcgcggcga tgtcgtggta gccgctgtcg tccttttcgt gaagcaggtc
     3301 ctcgtcgcaa atcaggtggt gcagtacgtc gtcgcgcggc tgccagccgg cctcgagccg
     3361 gtccagcacc aaggcgttcc gggctcggat cacctgctcg gctagccgga cgatgtcggc
     3421 atgtgccgcg ccgcggtgtt ctgcctcccg aaacgccgcg gtggcacgct gccaggcgtt
     3481 gaaaggtgcc gacggattcg gttgtgcact gctctgggtt tctgccacgt cggttcgctg
     3541 cctttgctcg gtactcgggg aattgaggcg cgaaagggtt tcttgtctga agcccaccag
     3601 aatgctacga cgacggattc cgtagggcaa tccgccgttc ggcactgtgc acgaggccac
     3661 agtcgctagc cctcgagtgc gggatgatga acgggtgagc gaatccgcga ggtcggggcg
     3721 acctgacggg gtctttgagc ctggagccat cgaggtcctt gagcgctggc agaacttcgg
     3781 cgcgacgtgg cgggtggtgt cgcgaatcga ggggtccgtc accctgtcac tgtgccgctg
     3841 cgacggcggg gaagaagttc aacggttgac gtcgagcgat gtcgacctgc ttgggtggct
     3901 cgctggcaga acgagcagcg agcagtagtc gatgtctcga catcaatctg catcccatgt
     3961 ttgcgctgtg acccgccact aatcgcttcg tagctcagaa gggccgctcc gtggcttcgt
     4021 ccgacacatt cgtgatcgtc ggtgccgggc tcgccggcgc gaaggcggcg gaggcgttgc
     4081 gcagccaggg ctttgatggt cggctgatcc tcctcggcgc cgagcaccac acgccatacg
     4141 agcggccgcc cttgtccaag gattacttga ccggcaaggc cccgcgcgaa aaggtcttcg
     4201 tccacgccca ccgctggtac gccgaccacg acgtcgagtt gaggtccggg cagttcgtga
     4261 ctgatctgga ccgtgcaacc cgcggtgtcg ggctagccga tgggtcggtt gtcagttacg
     4321 acaagctctt gctgactaca ggggcgagcc cgcgcgagct tggggtgccc ggggcagacg
     4381 cccgtggtgt gcactacctc cggaccctcg acgactccga tgtgttgcgc gggaccctcg
     4441 ccaccgcctc gcggttggtc gtgatcggcg ccggctggat cggattggag gtgaccgcgg
     4501 cggcccgcct agttggagtt gaggtcactg tcgtcgaaac ggctgcactg ccactgctgc
     4561 gcgtgctcgg gcccgaggtc gcccaggttt tcgccaactt gcatcgcgag cacggggtcg
     4621 agttccgctt cgacgcatcc ctcgccgaga tcgtcaccga caacgggcag gccaccggcg
     4681 tccggctgaa cgacggtacg acgatcgagg cagacgctgt tctggttgcg gtgggcgcga
     4741 ctccgaacgt tcaacttggg gaggctgcgg ggcttcgcgt tgacaacggc attgtcgtcg
     4801 acgccgcgct acggaccagc gaccccaaca tcttcgccgc gggcgatgtc gcgaacgcgt
     4861 tccacccgat gtacaaccga cacatccggg tcgagcattg ggccaacgcg cggcagcaac
     4921 cgaaagcggc cgcaacgtcc atgctcggaa atgacgcctc gtacgacgag ctaccgtact
     4981 tttacaccga ccagtacgac ctcggaatgg aatacgtcgg cttcgtcgag ccggacggct
     5041 acgaccaggt cgtcttccgc ggcgacgtcg cccgccgaga gttcatcgcg ttctggctct
     5101 cgaacaaccg cgttgtcgcc gggatgaatg tcaacgtgtg ggacgtggtt gagccgatca
     5161 aggcactcat ccgatcccgc gtcgcgatac cggcgggcag gctggccgac cgcggcgctg
     5221 ccctcatgga ccttgtgcag ccgagctaac ggctaggcgg catgccacgt tgcgggtcga
     5281 cggctttgca gggcggccgc aacggtgacc aacgcgagca ggaatagcgc caatgccaat
     5341 tcgcacg
//
LOCUS       DATCBJ010000560         4421 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_93296, whole genome shotgun sequence.
ACCESSION   DATCBJ010000560 DATCBJ010000000
VERSION     DATCBJ010000560.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 4421)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4421)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4421
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_93296"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(113..592)
                     /locus_tag="VII50_11245"
     CDS             complement(113..592)
                     /locus_tag="VII50_11245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5051468.1"
                     /translation="MAVTSGFRGALPGGGSRVTWALDLAQRGVSVDAPRSVRWLTFGG
                     VALGAAAVAASAVIHLHLWMQGYKHIHLIGPLFLAQAVAGIGLALAMVAYPRFVSAVA
                     GALYMASTIGGLLMSATVGIFGFHDGLRVPWATSSLVIEAAGLVVLVAAGAALFARR"
     gene            complement(598..822)
                     /locus_tag="VII50_11250"
     CDS             complement(598..822)
                     /locus_tag="VII50_11250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5051469.1"
                     /translation="MRQSVGVRWAAVLVLVVALAGCKSGTSSPASSTPSSGTATSAPA
                     SASCVIPQGNGGDHDADNNGGPDDGDGCDK"
     gene            complement(998..2131)
                     /locus_tag="VII50_11255"
     CDS             complement(998..2131)
                     /locus_tag="VII50_11255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5051470.1"
                     /translation="MNALRRGLVGLAGAGLVVGVAVPAASASGTFLGSLTSISTVAST
                     VPAHGAGAGDLNPYGVAVVPRTVNRLHAGSVLVSNFNAASNFQGTGTTIMQITAGGHA
                     DVFARVSGGHLPGSCPGGVGLTTALSVLSSGWVIVGSLPTTDGSAATAKAGCLLVLDS
                     HGIVRRTISGGPINGPWDMTALDHGNTADLFVTNVLNGAVAGEGAVVNRGTVVRIQLR
                     LHNTDVPVVTGETVIASGFGERTDSAALIVGPTGLALDRRHDVLYVADTASNRIAAVP
                     DAQTRTTSAFSGSDVTANGKLNNPLGLALAPNGNLLSVNADDGLIVETTQAGAQVASF
                     TLDTSGVPPATPGSGALFGLAIEPSGTGVYFVDDATNTLDLLH"
     gene            complement(2295..3062)
                     /locus_tag="VII50_11260"
     CDS             complement(2295..3062)
                     /locus_tag="VII50_11260"
                     /inference="COORDINATES: protein motif:HMM:NF017771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF899 domain-containing protein"
                     /protein_id="HEY5051471.1"
                     /translation="MTGHPRGTRAEWLAARLELLEAEKALTKRSDEVARQRRELPWVR
                     IDKEYQLETDEGTSSLTDLFRGRSQLLIYHFMFGPDYTAGCPSCSAIADGFDRSAVHL
                     ANHDVTFSAVSRAPLAKLQAYKKRMGWTFPWASSFDSDFNHDFQASNTKEEWESGAVE
                     YNFQTADLRPPAGDDSSNLDDFASSIVGTDWETYRREGPGMSAFALEDGVVYHTYSAY
                     ARGIDGLWGMYQWLDRAPLGRNEDGMWWRRHDEYDTQ"
     gene            complement(3116..3802)
                     /locus_tag="VII50_11265"
     CDS             complement(3116..3802)
                     /locus_tag="VII50_11265"
                     /inference="COORDINATES: protein motif:HMM:NF021465.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2182 domain-containing protein"
                     /protein_id="HEY5051472.1"
                     /translation="MTTASTAALSDARKTLQLRRAALTATLGLAAAAWVIAVRQMNGM
                     NMGVATTLGSFGFFVAVWVSMMAAMMLPAAAPAVLRRADASRRLRAVLGFVGSYLVVW
                     TLVGIAVYALYRPHGSVAAGAVVIAAGAYEWTPLKRRCRRRCRERIRSGFAFGLYCVG
                     SSIGLMLILVAVGVMSIAWMSVVAGVVLAQKLLPAKAAADVALALGIVGLGILIVIAP
                     SVVPGLTPAM"
     gene            complement(3833..4168)
                     /locus_tag="VII50_11270"
     CDS             complement(3833..4168)
                     /locus_tag="VII50_11270"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEY5051473.1"
                     /translation="MKAAVLERADELFAALADPNRRLLLEHLGAQGAATATMLAAKLP
                     VTRQAVGQHLAVLESVDLVSSARVGRERRYTVHVEPLTAAATWMNHVAAQWDARLAAI
                     KSLAEALAD"
     gene            complement(4165..>4421)
                     /locus_tag="VII50_11275"
     CDS             complement(4165..>4421)
                     /locus_tag="VII50_11275"
                     /inference="COORDINATES: protein motif:HMM:NF019928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HEY5051474.1"
                     /translation="PPHYLAYRWASAYPDTLADDTNSTLVEFRLTADEETTRLTVCES
                     GFATLTIPPGREGFASYDSHSQGWTEIVRNLADYVERGGP"
BASE COUNT          821 a         1480 c         1413 g          707 t
ORIGIN      
        1 cccatgggat cgaagcgcac accggtccgc gacgcgcatc cggcgcacgc caaacacgca
       61 ccgatcgatg cgacaacggc cgcgagcgtt gagccgagca tggtgggcgc tctcaacggc
      121 gcgcgaacaa cgccgctcca gccgccacaa gaacgaccag gccagcggcc tcgatcacta
      181 acgagctcgt agcccacgga acgcgtaagc cgtcgtggaa gccgaatatc ccgaccgtag
      241 cgctcatgag caggccacca atcgtcgacg ccatgtacag cgctccggct actgcgctga
      301 cgaacctggg gtaggccacc atcgccaatg cgaggccaat cccggcgacc gcctgggcga
      361 ggaacaatgg cccgatcaaa tgaatgtgct tgtagccctg catccacagg tgaagatgaa
      421 tcacggccga cgcagcaacc gccgctgcgc caagggcaac accaccaaag gtcaaccagc
      481 gcaccgatcg cggggcgtcg accgagaccc cgcgttgagc gagatccaac gcccacgtca
      541 cccggctccc gccgccgggg agtgcgccac gaaagccgga ggtcaccgcc atgggactca
      601 cttgtcgcac ccatcgccgt cgtctgggcc gccgttgttg tccgcgtcat gatccccacc
      661 gttgccctgc ggaatgacgc agctggctga cgccggcgcg ctggtggctg taccggaact
      721 cggtgtcgag gacgcaggcg aggaggtgcc gctcttacaa cccgccaacg cgaccaccaa
      781 caccagcacc gcagcccaac gcacacccac cgattgccgc atccgtttct ccttcgaatc
      841 agcgcaaacc tgcctcaaca ctcgtaatgc ggtgcgaaag ccacgctggg tttgccgacc
      901 gtccgaagcc gtccaacccg acagcgacgc aggaggaacg ccgtccgcgc tgaccaccga
      961 tttagacgcc gaacaacctg cgcggcgact cagttactca gtgcaagagg tcgagggtgt
     1021 tggtggcgtc gtcgacgaag tagacgcccg tgccgcttgg ttcgatggca aggccaaaca
     1081 gggcccctga ccctggggtc gctggcggaa cgccactggt gtccagggtg aaggaagcga
     1141 cttgggcacc ggcctgggtg gtctccacga tgagcccgtc gtcggcgttg accgacagca
     1201 ggttcccgtt aggggctagt gcgaggccca aggggttgtt caacttgccg ttggctgtca
     1261 cgtcggatcc gctgaacgcg ctcgtggtgc gcgtctgggc gtcgggaaca gccgcgatgc
     1321 ggttgctcgc ggtgtctgcg acgtagagca cgtcgtggcg gcggtccaac gccaggccgg
     1381 ttgggccgac gatgagcgcc gccgagtctg tgcgctcgcc aaatccggac gcgatgacgg
     1441 tctcgccggt cactaccggg acgtccgtgt tgtggaggcg cagctggatg cgcaccactg
     1501 tgccgcgatt gacgacggcc ccctcacccg caacggcgcc gttcagtacg ttggtcacaa
     1561 agaggtcggc ggtgttgccg tggtcgagcg ccgtcatgtc ccatggacca ttgattggtc
     1621 caccggagat ggtgcggcgc acgatgccgt gcgaatcgag caccaacagg cagcctgcct
     1681 tagctgtagc ggcgctcccg tcggttgtcg gcaaactccc tacgatcacc catcccgaac
     1741 tcagcacaga cagcgcggtg gtcaagccga caccgcctgg acagctcccc ggaagatggc
     1801 cgccgctgac ccgggcgaaa acgtcagcgt gtccgcccgc agtgatttgc atgatcgtgg
     1861 tgccagtgcc ctggaagttc gacgccgcgt tgaagttgct taccaacacc gagccagcgt
     1921 gcaaacgatt gacggtccgc ggcacgaccg cgacaccata cgggttcaga tcacctgcgc
     1981 ccgccccatg cgcgggcacc gtcgaagcca ccgtcgaaat cgacgtgagc gagccgagga
     2041 aagttccact ggccgacgct gcaggcaccg cgaccccaac aacgagccca gcaccggcga
     2101 gccctacaag tccacgacga agcgcgttca tgactcctcc aagtgcggac acctgacgcc
     2161 cgaacgggcc tcaacgcgag aacggcgcaa acggtcgccc ggttcaacgc gacggtccgg
     2221 cccgtgatgg ccggtgctct cagcgctcgc gccggccgac cgttcctacc ggtctcggcg
     2281 cgccacgaca ggtgtcactg cgtgtcgtac tcgtcatgtc gacgccacca catgccgtcc
     2341 tcgttgcggc ccaggggcgc gcgatcgagc cactgataca tgccccacag gccgtcgatc
     2401 ccgcgcgcgt acgccgaata ggtgtgatag acgaccccat cctcgagcgc gaaggcgctc
     2461 atgccggggc cttctcgccg gtatgtctcc cagtccgttc cgacgatgct cgatgcgaag
     2521 tcgtcgaggt tggagctgtc gtcaccggcc ggcggccgga ggtctgccgt ttggaagttg
     2581 tactcgacgg cgcccgactc ccactcttcc ttggtgtttg aagcctggaa gtcgtggttg
     2641 aagtcgctgt cgaacgaaga cgcccatggg aaggtccatc ccatccgctt cttgtatgcc
     2701 tggagcttgg cgagcggagc ccgtgacacg gcgctgaacg tgacgtcgtg attcgcgagg
     2761 tggacggcag agcgatcgaa cccgtcagcg atcgctgagc atgacggaca ccctgccgtg
     2821 tagtccggcc cgaacatgaa gtgatagatg aggagctggg agcgacctcg aaagagatca
     2881 gtgagggacg acgtgccctc gtccgtctcg agttggtact ccttgtcgat ccgaacccag
     2941 gggagctccc ggcgttgccg cgccacttcg tcgctacgct tcgtcagcgc cttctcggct
     3001 tcaagcagct ccagccgggc ggcaagccac tctgcgcgtg ttcccctcgg atgaccggtc
     3061 atgatttctc cttgctgtcg ctacaacgga ttggcgtcgt gcgcgccaca cgaggtcaca
     3121 tcgctggcgt gagtccagga accactgagg gcgcgatgac aatcaggatt ccgaggccga
     3181 cgatcccgag cgccaacgcg acatcggcgg cggccttcgc gggtaacagt ttctgagcga
     3241 ggacgacgcc ggcgaccacc gacatccagg cgatgctcat gacgcccacc gccaccagaa
     3301 tcagcatcaa tccgatgctt gaacccacgc agtagagccc gaacgcgaag ccagagcgga
     3361 tgcgctcgcg gcagcgccgg cgacaacgcc gcttcagtgg tgtccattcg tacgcaccag
     3421 ccgcgatcac aacggcaccg gcggcgaccg acccatgcgg ccggtacagg gcatacacgg
     3481 cgatgcccac aagcgtccac acgacaaggt acgaccccac gaatcccagt acggcgcgca
     3541 ggcgtcggct cgcatcagcg cgtctcaaca ctgccggagc agcggccggc agcatcatcg
     3601 ccgccatcat cgacacccag accgcaacga agaaaccgaa ggaaccgagc gtcgtcgcga
     3661 cgcccatgtt cataccgttc atctgccgta ccgcgatgac ccacgccgca gcggcgagtc
     3721 ccagggtggc ggtcagcgcg gcgcgtcgaa gttgtagcgt ctttcgcgcg tcgctcaagg
     3781 ctgccgtact tgccgttgtc atgggttact cctcatgtgt ggatgccggg cgctagtccg
     3841 cgagcgcctc ggccagcgac ttgatcgcgg cgagtcgggc atcccattgg gcggcgacgt
     3901 ggttcatcca cgttgccgcg gcggtgagcg gttcgacgtg cacggtgtag cggcgttcgc
     3961 gtccgacccg agcactcgag acgaggtcga cgctctcgag cacagcgagg tgttgcccga
     4021 ctgcctgccg ggtcacggga agcttggcag ccaacatcgt cgcggtggcg gcaccttggg
     4081 cgccgaggtg ttcgagaagt aggcgccggt tcgggtcggc gagcgcggcg aacaactcat
     4141 cagcgcgttc gagaacggcg gccttcatgg tccgcctcgt tcgacgtagt cggcgaggtt
     4201 gcgcacgatt tcggtccagc cctggctgtg actgtcgtaa gaagcgaagc cctcgcggcc
     4261 gggcgggatc gtcagggtgg cgaatccgct ctcgcacacg gtcaagcgcg tcgtctcttc
     4321 gtcggcggtg agacgaaact cgaccaaggt cgagttcgta tcgtcggcca gtgtgtccgg
     4381 ataggcgctg gcccaccggt aggcgaggta gtgcggcggg t
//
LOCUS       DATCBJ010000561         3907 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_158296, whole genome shotgun sequence.
ACCESSION   DATCBJ010000561 DATCBJ010000000
VERSION     DATCBJ010000561.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 3907)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3907)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3907
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_158296"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..291
                     /locus_tag="VII50_11280"
     CDS             <1..291
                     /locus_tag="VII50_11280"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY5051475.1"
                     /translation="YLEDLYVRPELRGHGYGGELLVELARVADERGYGRLEWAVLDWN
                     ESAHRFYASLGAVPMQEWTIWRVTGDALPKLAQRSTSVSTTGGTPPDAQGRT"
     gene            399..833
                     /locus_tag="VII50_11285"
     CDS             399..833
                     /locus_tag="VII50_11285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma regulatory factor"
                     /protein_id="HEY5051476.1"
                     /translation="MPVVGAEADAWPARDRLGGDFVCVRVPAAAAYLSVLRTTTASLA
                     ARLDFTLDEIEDLRIAVDEASCLLLAGAVEDAELTSVFTMSADSIQISVSTLTNKGHG
                     PSRDSFAWTVLSALAGEVDAWTEADGRQTISLIKHRSRDLLT"
     gene            830..1708
                     /locus_tag="VII50_11290"
     CDS             830..1708
                     /locus_tag="VII50_11290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor SigF"
                     /protein_id="HEY5051477.1"
                     /translation="MSTSPNVHADDGFKPAPPDVTPRSAPGDAIPVVTARSEQAASDR
                     ARARALFAQMVSLPEGSPERHAIRDQLVETHLPLVEYLARRFRNRGELHDDLVQVATI
                     GLIKSVDRFDLERGVEFSTYATPTIVGEIKRHFRDKGWAIRVPRRLQELKLALAKATG
                     ELSQRNGRSPTVGELAAFLGMSEDDVLEGLESANAYSTVSLDTPESGDSDALAVADTL
                     GVIDESLEGVEYRESLKPLLDQLPPREKRILLLRFFGNMTQSQIAIDLGISQMHVSRL
                     LARTLAQLREGLLVEE"
     gene            complement(1712..2125)
                     /locus_tag="VII50_11295"
     CDS             complement(1712..2125)
                     /locus_tag="VII50_11295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5051478.1"
                     /translation="MSKSDASESEPLGIPLLLAVIATAVETVGLAASAIGLSIYKLSG
                     HRPFDSGDLWAVVAMAVVGAIGLGLVVRGLVRARRWARSPALLTQLIVFPIGVTTIVH
                     GGDLVGIPLAVCGLVGVVGLFAPSTTQRLNRTYDG"
     gene            2198..3130
                     /locus_tag="VII50_11300"
     CDS             2198..3130
                     /locus_tag="VII50_11300"
                     /inference="COORDINATES: protein motif:HMM:NF012981.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HEY5051479.1"
                     /translation="MRALLVANLNATAMTPRTREVIVRALGSELKIDVAETDSRGHAA
                     DLAAEARADQFDLVVAVGGDGTVNEVVTGLLRDGVGADTPRLAVVPGGNANVFARALG
                     LPSEPIEATGAVIERLRAGRERRIGLGRLTTAGLDRWFTFCAGFGLDAEIVREVERLR
                     RDGAKATPALFMRTGLRQFFRTDRRHPALVLETPGLDPVEKLFMTIVTNTTPWTYFGD
                     RPLEPTPHASFESGLDAFSLRRLGTARTGNNLRRLLVGGPTPKGRSIVSLHDLPALTL
                     RTTHPIPIEVDGEYLGEHSDPVLFSVPEALAVIA"
     gene            3564..3815
                     /locus_tag="VII50_11305"
     CDS             3564..3815
                     /locus_tag="VII50_11305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WhiB family transcriptional regulator"
                     /protein_id="HEY5051480.1"
                     /translation="MDWRHRAACRDEDPELFFPIGNTGPALLQIDQAKAVCRRCPVAE
                     PCIAWALETGQDSGVWGGLSEDERRALKRRGARLRPQTV"
BASE COUNT          724 a         1342 c         1237 g          604 t
ORIGIN      
        1 tacctcgagg acctctatgt gcggccggag ttgcgcggcc acggctacgg cggcgagctc
       61 ctcgtcgagc tcgcccgggt cgccgacgag cggggctacg gccgcctcga gtgggcggtc
      121 ctggactgga acgaatccgc gcatcgcttc tacgcgtcgc tcggcgccgt cccgatgcag
      181 gagtggacca tctggcgggt caccggcgat gcgttgccga agctcgccca gcgatccacc
      241 tctgtcagca ccacgggcgg gacaccgccg gacgcccagg ggcgcaccta gtcacgcaca
      301 gttcggcgcc tgcgcggtac cgtgaagccc ggccgggagc agcaaagctg gcaaacctgc
      361 atacagatgg agcgcgacgc gtgtcgacga cagagggcgt gccagtagtc ggcgcggaag
      421 cagacgcttg gccggcccga gaccgcctcg gtggcgactt tgtgtgcgtt cgggtgccag
      481 ccgccgcggc ctacctctcg gtcctgcgca cgacgaccgc aagcctcgcg gcgcggctcg
      541 acttcaccct cgacgagatc gaggacttgc gcatcgcggt cgacgaggcg tcctgcctgc
      601 tgctggctgg cgcggttgag gacgcggagc tgaccagcgt gttcacgatg tccgccgact
      661 caatccagat ttccgtgtcg accctgacga acaagggcca cggccctagc cgggactcgt
      721 tcgcgtggac cgtgttgtca gcactggccg gcgaggtcga cgcatggacc gaggccgacg
      781 ggcggcagac gatctcgctg atcaaacacc ggtcgaggga cctgctgacg tgagcaccag
      841 ccccaacgtg cacgcggacg acgggttcaa gcctgcaccg ccggatgtga ccccgcgctc
      901 agcacctggc gacgcgatcc cggtcgtcac cgcgcgatcg gagcaggccg cgagcgatcg
      961 cgcgcgcgcc cgggcgcttt ttgcgcagat ggtgagcctg ccggaaggct cccccgagcg
     1021 gcacgccatt cgtgaccaac tcgtcgagac acacttaccc ctcgtcgagt atctggcacg
     1081 ccggttccgc aaccgcggcg agctgcacga cgacttggtg caggtggcaa cgatcggact
     1141 gatcaagtcc gttgaccggt tcgacctgga gcgtggcgtc gaattctcga cgtacgcaac
     1201 gccgaccatc gtgggtgaga tcaagcggca tttccgtgac aaaggttggg cgatccgcgt
     1261 cccgcggcgg ctgcaggagt tgaagctcgc actcgcaaaa gcgaccggtg aactctccca
     1321 acgcaacggt cggtcaccga cagtcggcga gctcgccgcg ttcctgggca tgagtgaaga
     1381 cgacgtactg gaaggcctcg agtcggcgaa cgcgtactcc acggtctccc tcgacacacc
     1441 cgagagcggc gactccgatg cgctggccgt ggccgacaca ctcggggtca tcgacgagtc
     1501 gctcgagggc gttgagtacc gggagtcgct gaagccactc ctcgaccagt taccgccccg
     1561 cgagaagcgc atcctgctgc tgcgtttctt cggcaacatg acgcaatcgc agatcgccat
     1621 cgacctcggc atctcacaga tgcacgtgtc gcgattgctg gcccgcaccc tcgcccagct
     1681 gcgcgaagga ctgctcgtcg aggagtagag ctcagccgtc gtacgtgcgg ttcagacgtt
     1741 gtgtcgtgga cggcgcgaag aggccgacca ccccgaccaa tccgcatacc gcgagcggaa
     1801 tgccgacaag gtcacccccg tgcacgatcg tggtcacgcc gatcgggaac acgatgagtt
     1861 gcgtcaatag ggctggcgat ctcgcccagc gccgggcacg aaccaatcca cgaaccacaa
     1921 ggccaagacc gatcgcaccg accaccgcca tcgcaacgac tgcccacaga tcgcccgaat
     1981 cgaagggccg atgccctgac aacttgtaga tcgacaagcc gatggcggac gccgcaagtc
     2041 cgacggtctc gacagccgtg gcgatgacag caagaagcag cgggatgccg agtggctcgg
     2101 actctgacgc gtcggacttt gacacgaggc gaagatagcc ggtgcgccga atgccgggtg
     2161 ttgccgagag cacagaccag cggcgtaccc ttgggaaatg cgagcgctgc tggttgccaa
     2221 tctgaatgca accgccatga cgccgcgcac gcgtgaagtc atcgtccggg cccttggcag
     2281 cgagctgaaa atcgacgtcg cagagaccga cagtcgtggg cacgccgctg atctggccgc
     2341 cgaggcacgc gccgatcagt tcgaccttgt cgtcgcagta ggtggcgacg gcaccgtcaa
     2401 cgaggtcgtc accggactgc tgcgagacgg agtcggggca gacaccccgc gccttgcggt
     2461 ggtgcctggc ggaaacgcga acgtcttcgc ccgcgcgctc ggcctgccct ccgaaccgat
     2521 cgaagcgacc ggcgccgtga tcgagcggct tcgggccggt cgcgagcggc ggattggctt
     2581 gggccggctc acgaccgccg gactggatcg ctggttcacg ttctgtgcgg gatttgggct
     2641 cgacgccgag atcgtccgcg aggtcgagcg gcttcgacgt gacggcgcga aggccacccc
     2701 cgccctgttc atgcgcaccg gcctacgtca gttcttccgg acggaccggc gccacccggc
     2761 gctcgtgctg gagacgcccg gcctggatcc agtcgagaag ctgttcatga cgatcgtgac
     2821 gaacaccacg ccctggacct atttcggtga ccggccactc gaacccacac cacacgcatc
     2881 gttcgagtcc ggcctcgatg ccttttcgtt gcgtcggctc ggcaccgcac ggaccggcaa
     2941 caacctgcgg cggttgctcg tcggaggccc gaccccgaag gggcgcagca tcgtctccct
     3001 gcacgacctg cccgccctca cactgcgcac gacgcacccg attccgatcg aggttgacgg
     3061 cgaatatctg ggcgagcact ccgatccggt gctcttctcc gtccccgagg cgctcgccgt
     3121 catcgcctga cgccgcaacg cccagcgctc ggcatctcgc agcgcagccc tagctgtgtc
     3181 ctaagccacg tgtacaaatc gccacctgga cgaaatagac gaggcgcgac ccttgcgaag
     3241 ggctcgggcg cctgatttag tcgagggcgg aacggcgttg accgacaagc tcgtgaacgt
     3301 gttcacaagc aagcgtttca aaaccaccca cacctgggta atggcgtggg tactgctaca
     3361 aggcagacat gtttagcgct ccaccgaatg acgcagacct agcccaaccg aacatagcca
     3421 cacacgggga tctcccaacg acccgaacca ccgaatgatc cgaaccaccg aatgatccga
     3481 accaccgagc cccctgcacc accgtgcccc cacgagtaga tgagcgcggg actgtcccgc
     3541 tgactgctga ggagatggac cacatggact ggcgccaccg cgctgcttgc cgtgacgaag
     3601 accccgaact cttcttccca atcggcaaca ccggcccagc ccttctccag atcgatcagg
     3661 cgaaggcagt ctgccggcgt tgcccggtag ccgaaccatg tattgcgtgg gcgctcgaga
     3721 ctggccagga ttccggcgtc tggggcggac tgtctgaaga tgaacgacgt gcgctcaagc
     3781 gccgtggcgc tcgactgcgc ccccagaccg tctaatctcc gcacgccagt aatccctgcc
     3841 gcacaagtcc gcctgtcgca ctactccgcc aacggcactt ccgccaacgg cacggggatt
     3901 cgccacg
//
LOCUS       DATCBJ010000562         2542 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_129308, whole genome shotgun sequence.
ACCESSION   DATCBJ010000562 DATCBJ010000000
VERSION     DATCBJ010000562.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2542)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2542)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2542
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_129308"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..198)
                     /locus_tag="VII50_11310"
     CDS             complement(<1..198)
                     /locus_tag="VII50_11310"
                     /EC_number="3.6.1.7"
                     /inference="COORDINATES: protein motif:HMM:NF012911.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HEY5051481.1"
                     /translation="MPTPPRLGRRIAVHGTVQGVGFRPFVYRLAVELGLEGTVGNADG
                     HVVIDAAGTQDALGEFVRRVAQ"
     gene            477..902
                     /locus_tag="VII50_11315"
     CDS             477..902
                     /locus_tag="VII50_11315"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEY5051482.1"
                     /translation="MTTPEIARPLIVVGVDGSPQSQKAIAWGVEQAKKTGGTLELLIA
                     WARPVSYGLPLVVAGLDPEAQAREIVEKAATDVDLPANRVITNVVHGSAPSTLVERSS
                     QADLLVVGSRGHGGFAELLLGSVSAQAVHHAACPVVVIR"
     gene            978..>2542
                     /locus_tag="VII50_11320"
     CDS             978..>2542
                     /locus_tag="VII50_11320"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024994.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY5051483.1"
                     /translation="MQIPDVDVDALLVDAGIARIRTIRPTDAAALGCLYDRASGDSLH
                     MRFFSVSELNSRRDVERMTRPPADDHGSLVAQVRGEVVGVASFERLDDDPSVAEFAML
                     VDDAQHGRGIGMLLLEHLSALATLYGVTRFVAEVLPGNASMLHLFRDAGLPMAKQSGT
                     NVVHVELPLQFDGCFRRAVGIREARADAKSMARILAPASVVVVGAGIDPAGVGHQVLA
                     NIVRGGYQGDLYVVNRSGHRVAGIRAHRSLAELARPIDVVVIAVPAGEVLGVVNEAAA
                     CGAAGVVVVAAGFAELGLAGQLRQDELVRACRAAGMRLIGPNCSGIANAGPAVALNAT
                     LSPTMPAGGGIGLMAQSGAVGTAALAYAARVGVGISSFVSAGNKADVSGNDLLCAWEA
                     DSATRVCALYLESLGNPRKFARIAARVGRSKPVVVVKSGRSEPAGHGGGSRLAAAATA
                     DVAADTLFEQAGVTRVESLAELFDVATVFDLAPLPTGGRVAIVANGAGPGLLAADACA
                     AAGLDVVALGNVTR"
BASE COUNT          401 a          814 c          851 g          476 t
ORIGIN      
        1 ttgggcgacg cggcgcacga actcgcctag cgcgtcctgc gttcccgcag cgtcgatcac
       61 cacgtggccg tcagcgttgc cgacggttcc ttcgaggccc agttcgacag ccagccggta
      121 aacaaagggg cgaaacccga caccctgcac cgtgccatgg accgcgatcc gccgaccgag
      181 ccgcggcggt gtaggcaccg cacccggcct cgccgaaggc accgtcatca ccgacccgtc
      241 gtccataaag caacgatcga tggccggacc gtgcggtaac gagagtcatc cgtccgcagc
      301 cgccggactt tggtccctaa cggctcacac ttctcgctga gcacaggtgg tcccccctgg
      361 tcggccctcg gcccagcctg cgacgaggga cctagcggcc gagcaataag gaccaatggc
      421 cgtaaccccg acccgtctac gggcaagatc ctaggttcac gatacgagga ggactaatga
      481 caacacccga aatcgcccgg ccgctcatcg tcgtcggcgt cgatggatcg ccgcaaagtc
      541 agaaggctat cgcctgggga gtcgagcagg ccaagaagac cggtggcacc ttggagctct
      601 tgatcgcctg ggcgcgaccg gtgtcctatg ggctaccgct cgttgtcgcc gggcttgacc
      661 ccgaggcaca ggcacgcgag atcgtcgaga aggcggcaac agatgtggac ctgccggcca
      721 accgtgtgat caccaacgtc gtccatggat ccgcgccatc gaccctcgtc gaacgcagct
      781 ctcaggccga tctgctcgtt gtgggctcgc gcggacacgg tggattcgcc gaactattgc
      841 tcggttcggt cagcgcgcaa gcggttcacc acgcggcgtg ccccgtcgtc gtcattcgat
      901 agctggtccg ggctcgtcgc tttggcccgc ctcggtgcgc ggtcagcgct cgacacgcct
      961 gggaggagat cgtgaccatg cagatacctg acgtggacgt ggatgcactt ctcgtcgacg
     1021 ccggcatcgc gcggattcgc acgattcgcc ctactgacgc ggcagccctt ggttgcctgt
     1081 acgaccgcgc ttccggggac tcgcttcaca tgcgcttctt ctcagtttcg gaattgaact
     1141 cccgccgcga cgtcgagcgc atgacgcgtc ctcctgctga cgaccacggc tcgttggtgg
     1201 cgcaggtccg tggcgaggtg gtgggagttg catcgttcga gcgactcgac gacgatccca
     1261 gcgtcgccga atttgccatg ctcgtcgacg acgctcagca cggtcgtggt atcggcatgc
     1321 ttctgcttga gcacctcagc gcattggcaa ccctgtatgg cgtcacccgg tttgtagctg
     1381 aggtgcttcc tggaaacgcc tcgatgctcc atctgttccg cgatgcgggc ttgccgatgg
     1441 cgaaacagag cggaaccaat gtcgtacacg tcgagctgcc tttgcagttt gacgggtgct
     1501 tccgccgcgc cgtcggtatt cgcgaggctc gagcggatgc caaatcgatg gcgaggatcc
     1561 tcgcgcccgc ctcggtcgtg gtcgtcggcg caggtattga ccccgcaggt gtgggccacc
     1621 aagtgctcgc caatatcgtt cgcggcggct atcagggaga cctgtatgtc gtcaacaggt
     1681 cggggcaccg ggtggcgggc atccgcgcgc atcgatccct ggccgaattg gctcggccta
     1741 tcgatgttgt cgttatcgca gttccggcgg gcgaggtcct cggcgtcgtg aacgaggccg
     1801 ccgcgtgcgg cgccgctgga gttgtcgtcg ttgcggcggg tttcgccgag cttggtctcg
     1861 cggggcagtt gcgtcaggac gaacttgtgc gggcgtgccg ggctgctggc atgcgcttga
     1921 tcggtccgaa ctgctcgggg atcgccaacg caggcccggc ggttgccctg aatgcgacct
     1981 tgtcgccgac gatgcccgcg ggcggcggga tcggactgat ggcgcaatca ggcgccgttg
     2041 gcactgccgc gttggcgtac gcggctcgcg tcggtgtcgg aatctcgagc ttcgtctctg
     2101 cgggcaacaa ggcggatgtt tcgggcaatg acctgctgtg cgcgtgggag gcggattcgg
     2161 ccacccgagt atgtgcgttg tacctcgagt cgctcggcaa cccgcgcaag ttcgcccgca
     2221 tcgcggcgag agtagggcgc agcaagccgg tggttgtcgt caagtctgga cggagcgagc
     2281 ctgcaggcca cggtggtgga tcacgactcg cagcggcagc gaccgccgac gttgcggccg
     2341 acacgctgtt tgaacaagca ggcgtcacgc gagtggagtc acttgctgaa ttgttcgatg
     2401 tcgcaacggt gttcgatctg gcgccgcttc ccaccggagg ccgggtggca atcgttgcca
     2461 atggcgccgg cccgggtctc ctggctgccg acgcctgcgc cgcggctggt ctcgatgtcg
     2521 tggcattggg caacgtgacg cg
//
LOCUS       DATCBJ010000563         3090 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_353943, whole genome shotgun sequence.
ACCESSION   DATCBJ010000563 DATCBJ010000000
VERSION     DATCBJ010000563.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 3090)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3090)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3090
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_353943"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>3090
                     /gene="mfd"
                     /locus_tag="VII50_11325"
     CDS             <1..>3090
                     /gene="mfd"
                     /locus_tag="VII50_11325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HEY5051484.1"
                     /translation="VVTPVRALVQPQVAGLGDLEPVALQVGDEAVPDEVVARLIAAGY
                     TRVDLVERRGEIAVRGGILDVFPPTEDHPLRVEFFGDEVEEIRVFKAADQRSLGDDFP
                     ALRGLWAPPCRELLLTPDVLERAASLAAKHPQLADILGKVAHGIAVEGMESLASVLVD
                     KMDLLLDEMPPDTVVLLCDPERVRARAAEVVRTGEEFLQAAWAGAASGGKAPIDLSSA
                     ALRPLGDVRAHALSLGLGWWSMSPLAATADDTSAEASQVDADVLVIDAIAAQAYRGDT
                     QAVVADVKGWLADGWRVLFTTAGHGPAQRIAEVLRGEGVAARLEFELLGVPEPGAVVV
                     SCGALDAGFEAPAAKLVLLTENDVFGQRTSTRDLAKLPSRRKRSIDPMQLQPGDLVVH
                     EQHGVGRFIEMVQRTVAGATREYLVIEYAPSKRGQPGDRLYAPTDALDQVTRYVGGEN
                     PTLDRMGGSDWAKRKGRARKAVKEIAAELIRLYSARTAAPGYAFGPDTPWQRELEEAF
                     PYVETPDQLRTIDEVKADMELPIPMDRLICGDVGYGKTEIAVRAVFKAVQDGKQAAVL
                     CPTTLLVQQHFATFSERFANFPVVVRPVSRFNSLKEEQAIMAGVADGSVDVVIGTHRL
                     IAGSTHFKNLGLVVIDEEQRFGVEHKERLKQLRTNVDVLAMSATPIPRTLEMAITGIR
                     EMSVIQTPPEERHPVLTFVGGYDDRQVGAAIRRELLREGQVFYVHNRVESIERAASRL
                     RELVPEARIAVAHGQMNEDTLEQVMIGFWERDYDVLVCTTIVESGLDIPNANTLILER
                     ADLLGLSQLHQLRGRVGRGSERAYAYFLYPVERPLTETAHDRLATIAQNSDLGAGIAI
                     AMKDLEIRGAGNLLGGEQSGHIAAVGFDLYVRLVGEAVADFKGESSQELAEVRIDLPV
                     DAHIPHEYIDGERLRLAAYRAMAEATTAADVEAVREELADRYGPLPEPVSNLLEVAKL
                     RVELRPYGITEVVLQGNQIRFSPVTLRESQVLRLQRLFPKSIVKEPIATIMVPRPTD"
BASE COUNT          513 a          975 c         1056 g          546 t
ORIGIN      
        1 tcgtcgtcac gccggtgcga gcgctcgtcc agccgcaggt cgccggcctt ggtgacctcg
       61 agccggtcgc gctgcaggtc ggcgacgaag ctgtgcccga tgaggtagtc gcccggctga
      121 tcgcggccgg ctacacgcgg gtcgacctgg tcgagcggcg cggcgagatc gccgtgcgcg
      181 gcggcatcct cgatgtgttc ccgccgaccg aggaccaccc gctgcgagtt gagttcttcg
      241 gcgacgaggt cgaggagatc cgcgtcttca aggccgccga ccagcgcagc ctcggggacg
      301 acttcccggc tttgcgtggc ctctgggcgc cgccgtgtcg cgagttgctg ttgacgccag
      361 acgtgctcga gcgtgcagcc tcattggcgg cgaagcaccc gcaacttgcc gacattctcg
      421 gcaaggtcgc gcacggcatc gcggtcgagg gcatggagtc actcgcctcg gtcctggtcg
      481 acaagatgga cctgctgctc gacgagatgc cgcccgacac cgtggtgctg ctctgcgatc
      541 ccgaacgcgt gcgggcgcgc gccgccgagg tcgtgcgcac cggcgaagag ttcctgcagg
      601 cggcgtgggc cggtgccgcg tccggaggca aggccccgat cgacttgagc tcggcagctt
      661 tgcggccgct cggcgacgtc cgcgctcatg ctctgtcgct gggccttggg tggtggtcga
      721 tgtcgccact ggccgcgacg gctgacgata cgtccgccga agccagccag gtggacgccg
      781 atgtgctggt catcgatgcc atcgcggccc aggcatatcg cggtgacacg caggcggtgg
      841 tcgctgacgt caaggggtgg ctcgccgatg gctggcgggt gttgttcaca acggccggcc
      901 acggacccgc acaacgcatc gccgaggtgt tgcgcggtga gggcgtcgcg gcccggctcg
      961 agttcgaatt gctcggcgtg cccgaacctg gcgcggtcgt cgtatcctgc ggcgcgctcg
     1021 acgctggatt cgaagcacct gccgcgaaac tcgtgctgct taccgagaac gacgtcttcg
     1081 gccagcgcac gtcgacccgc gatctggcga agctgccaag ccggcgcaag cggtcgatcg
     1141 accccatgca gttgcagcca ggcgacctcg tcgtgcacga gcagcacggt gtcggccgct
     1201 ttatcgaaat ggtgcagcgc acggttgccg gcgcgacccg cgagtacctg gttatcgagt
     1261 acgcgccaag taaacgtgga caacctggtg accggctcta cgcgccgacc gatgcacttg
     1321 atcaggtcac gcgttacgtc ggtggcgaga atccgacgct cgaccgcatg ggcggctccg
     1381 actgggcgaa acgcaagggt cgggcgcgca aggctgtcaa ggagatcgcg gccgagttga
     1441 tccgtctcta cagtgcgcgc acggccgcgc ccggttatgc attcggtccc gataccccct
     1501 ggcagcgcga actcgaagag gcattcccct acgtcgaaac gccggaccag ttgcgcacga
     1561 tcgacgaagt caaggccgat atggaattgc cgattccgat ggatcggctg atctgcggcg
     1621 acgtcggcta cggcaagacc gagatcgctg ttcgcgccgt attcaaggcg gtgcaggacg
     1681 gcaagcaggc ggccgtgtta tgcccgacga cattgctcgt gcagcagcat ttcgcgacgt
     1741 tctccgaacg gttcgccaac ttcccggttg tcgtgcggcc ggtgagccgc ttcaactcgt
     1801 tgaaggagga gcaggccatc atggccggcg ttgctgatgg cagcgtcgac gtggtgatcg
     1861 gcactcatcg attgatcgcc gggagcacgc atttcaagaa tctcggcctc gtcgtgattg
     1921 acgaggagca gcggttcggc gtcgagcaca aggagcggct caagcagttg cgcacgaatg
     1981 tcgatgttct tgccatgtcg gcgaccccga ttccgcgcac gctcgaaatg gccatcaccg
     2041 gtattcgcga gatgtcggtg atccagacgc cgcccgaaga acggcaccca gtgctgacgt
     2101 tcgtcggcgg gtacgacgat cgtcaggtag gtgccgccat tcgccgcgag ttattgcgcg
     2161 agggccaggt tttctacgtc cacaacaggg tcgagtcgat cgagcgggcc gcttcccgtt
     2221 tgcgcgagct ggtgcccgag gcacgcatcg ccgttgcgca cggccagatg aacgaggaca
     2281 ccctcgagca ggtcatgatc ggattctggg agcgcgatta cgacgtgttg gtgtgcacca
     2341 caattgtcga gtcgggactc gatatcccga acgcgaatac gttgatcctc gaacgcgccg
     2401 acctgctcgg tctctcgcag ttgcatcagc tgcgtggccg ggtcggccgc ggcagtgaac
     2461 gtgcgtatgc gtacttcctt tacccggtcg aacgaccgct gactgagacc gcgcacgatc
     2521 ggctcgcgac gatcgcgcag aactccgacc tcggcgccgg tatcgcgatc gcgatgaaag
     2581 acctcgagat tcgtggcgcc ggcaacttgc ttggcggcga gcagtccgga cacatcgcgg
     2641 cagtcggctt cgacctctac gtccggctcg tcggtgaggc ggtcgcggat ttcaagggcg
     2701 agagctcgca ggagctcgcc gaggtcagga tcgaccttcc cgtcgacgcg cacatcccgc
     2761 acgagtacat cgacggcgag cggttgcggc tggccgcgta ccgggcgatg gccgaagcaa
     2821 caacagcggc ggacgtcgag gcggtgcgcg aggagttggc cgaccgttac ggcccgttgc
     2881 cggagcccgt gagcaacttg ctggaggtcg cgaaactgcg cgtcgagttg cggccgtacg
     2941 ggattaccga ggtcgttctc caggggaacc agattcggtt ttcgcccgtc accctccggg
     3001 aatcccaggt cttgcggctg cagcggcttt tcccgaaaag cattgtcaag gagccgattg
     3061 cgacgatcat ggttccacgg ccgactgatg
//
LOCUS       DATCBJ010000564         5773 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_199704, whole genome shotgun sequence.
ACCESSION   DATCBJ010000564 DATCBJ010000000
VERSION     DATCBJ010000564.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 5773)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5773)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5773
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_199704"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..571
                     /locus_tag="VII50_11330"
     CDS             <1..571
                     /locus_tag="VII50_11330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5051485.1"
                     /translation="PEHLVAEPIRNREAPATSSGLAFCGRTAPRRAPERRKEAEMPAK
                     NAKPESDGFSAEERAAMKERAAELRAEGRQGAKKADGLQEVLDKIAEMAPKDRVLAER
                     VHMTVTANAPELSPKTWYGMPAYANADGKIVVFFQDSGKFKYRYSTLGFQDAANLDDG
                     DLWAVSYALQKWSPVVEKKVAELVKAATS"
     gene            1208..1621
                     /locus_tag="VII50_11335"
     CDS             1208..1621
                     /locus_tag="VII50_11335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5051486.1"
                     /translation="MSESVEVLLPSPTIRSRRRGWIVAVVIVVVLGLGGGGFAAYRWL
                     TGGHLFVGPGASESLSLQAGHTIYVGLEPLPADPNPNATIRVDLRRVHPLVRTNTSDA
                     TVTAVEVAGAQVTYRVGIRGGNQDIGAVDLTVNTP"
     gene            1711..2394
                     /locus_tag="VII50_11340"
     CDS             1711..2394
                     /locus_tag="VII50_11340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5051487.1"
                     /translation="MAISLPAALVAGTSHTSRAGEGSAPAPSVSPTLSATVSAQSTTA
                     GSSARPTSPSSTDILGASVWNSHVLVITGQSLGGVTRGMSSAAAAHAAGVAAFTEVGD
                     GVLQPSGPLGSSQPRLYLSNFAGQPAASVGGDFSCVEASGSASGTSSVVTTRGPRLGD
                     PGTRVREIYGAAANFIPMPTTGGIDPNPGYVVHQGQFDLVFKLDASESHVIAIAGGLA
                     PMTPSECNG"
     gene            complement(2421..3344)
                     /locus_tag="VII50_11345"
     CDS             complement(2421..3344)
                     /locus_tag="VII50_11345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA lyase"
                     /protein_id="HEY5051488.1"
                     /translation="MSDLPTRISLREVGPRDGLQNEAPVPTAAKVELVDALSRTGVRR
                     IETVSFVLPRAIPQMADADDVWASIEHVPSITYSALVPNLRGAVRALDAGCRVIEIVV
                     SASDTHNRANVNRSTAESLDDIARVISLAHEQDAVCHVTVATAWGCPYEGEVPVSRVV
                     SVASRAVADGADGLSFGDTTGMATPTRVTELVGSMRMAVPDVPLNLHFHNTRGTALAN
                     VLAALQLGVADFDASVGGLGGCPYAPGASGNLATEELVYMVEDMGVATGIDLEVLIEV
                     AAQAERIVGRELPSQVLRAGPRTRRSTYPPS"
     gene            complement(3341..4150)
                     /locus_tag="VII50_11350"
     CDS             complement(3341..4150)
                     /locus_tag="VII50_11350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEY5051489.1"
                     /translation="MSDVPSPDAGQSGVLMWRPSEHVVELILDRPGALNAVSTELAAH
                     LGGVLRALAADAAVRAVVLSSSQERAFCVGADLKERAGFSNEQLLAQRPFIRAMFGSL
                     LELPMPVIAAVGGFALGGGFELALSCDLIVADESAVFGLTEVTVGLVPGGGGTQLLAR
                     RIGAGRAADLVLTGRKVGAQEAFRLGIVDRLTVDVHVRQLALDIAATIEGNSPLAVRH
                     AKHAMRTGFGTPLHEGLEIEDAAWRAAAMSADRREGISAFTEKRAPRWSGQ"
     gene            complement(4173..4754)
                     /locus_tag="VII50_11355"
     CDS             complement(4173..4754)
                     /locus_tag="VII50_11355"
                     /inference="COORDINATES: protein motif:HMM:NF015648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HEY5051490.1"
                     /translation="MGMNLSDGEEVVLDLHPHWGRLFVPVLVLLVACLLAGFGVALIP
                     KGGGQQIERWILIGVAAVVVIWLTILPYLRWLTTKYVLTTDRLVIRNGILARHGRDIP
                     LNRINDVSFSETVLERMLRSGTLVVESAGDNGQISLTDVPKVEHVQREVYRMVDQNLG
                     RSGPMSVADGGRAGRGGPAGTHPTAGDDASEQG"
     gene            4824..5375
                     /locus_tag="VII50_11360"
     CDS             4824..5375
                     /locus_tag="VII50_11360"
                     /inference="COORDINATES: protein motif:HMM:NF024812.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsinothricin resistance N-acetyltransferase
                     ArsN1 family B"
                     /protein_id="HEY5051491.1"
                     /translation="MADDSTVRAATTADAAAIAGIYRPYVLETVISFEETPPTAADFE
                     ARMLATPRLPWLVAVRADTAVGYCYASRHHTRASYRWSADVSVYVAQSERGRGAGRLL
                     YSELLPLVRALGYVSVFAGITLPNDASVGFHEAFGLTPVGVYRNVGFKHNAWRDVGWW
                     QLTFTQPPDEPAEPKPWLPPDVT"
     gene            complement(5368..>5773)
                     /locus_tag="VII50_11365"
     CDS             complement(5368..>5773)
                     /locus_tag="VII50_11365"
                     /inference="COORDINATES: protein motif:HMM:NF014310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5051492.1"
                     /translation="RKLAGILAEVVDDAVVVGVGLNVTLRDAELPVPTAISLALAGAS
                     CTDRDTLLRAILRGIGDEYLAWRRVDGDGERSVLPRYRELCVTLGRDVSVDLGGESLQ
                     GRALDINVDGSLVVETSTGRRSVSAGDVVHVR"
BASE COUNT         1029 a         2040 c         1821 g          883 t
ORIGIN      
        1 ccctgagcac ctggtcgccg aaccgatccg gaatcgagaa gcgccggcga catcgtcggg
       61 cctagccttc tgcgggagga cggcaccacg ccgtgccccc gagcgccgga aggaagccga
      121 aatgcccgcg aagaatgcca agcccgaatc cgacggattc agcgccgagg aacgtgccgc
      181 gatgaaggaa cgtgccgccg aactgcgggc tgagggcagg cagggcgcca aaaaggccga
      241 tggtttgcag gaagtcctcg acaagatcgc ggagatggca cccaaggatc gtgtgctcgc
      301 tgagcgagtg catatgacgg tgaccgcgaa cgcccctgag ctatcgccaa agacctggta
      361 cgggatgccc gcgtacgcga acgccgacgg caaaatcgtg gtcttcttcc aagactcggg
      421 caagttcaag tacaggtact cgaccctcgg cttccaggac gcagcgaacc tcgacgacgg
      481 cgacctgtgg gcggtgtcgt atgcgctcca gaagtggagc ccggtggtgg agaagaaggt
      541 cgccgaactg gtgaaggcag cgacctcctg actattgccg agcccggccg tcagggcccg
      601 gttgccatgg acatccgata gggcgacccg gcgttgggcc gagtgaaagt gatgaagcgt
      661 gcgttgaatc cgtcgtgcca gttcccaagt ccagtttcgt cgcggaaggc gaggtcgagg
      721 gtgaccatcg gcgtgaagtc atcgggcgag atcccgaggg ctggtagcgg gcgacggttg
      781 cccgcacgag gggagcagac tccgactggg ttgttgccga cgttgtcccg gcgtcgggct
      841 ggcattgctc ggtgggcgtc aggtaggcgg ccgccgcggc gcacgggccg tcagagccac
      901 cgggtgagga aggaacggac gtcgtcgagc gccgccgcca caacgcgttc gttcgcaccc
      961 gacgtcgctg gtccagccga accgttttcg ggcgtgcatg ctgcggttgc ggcccgcctt
     1021 accatggcac acgcgctcag tcgaaagagt gaccccgggc gcgcgttgtt catcgtgcct
     1081 cctgacgtgg ccatctgccg acaacgtccc gccatgaccg ccaccaccta tacgcctcag
     1141 ttccgtggac ggtttgccca acaccacggc ctaggccacg tcgaataggt cgacaaacgg
     1201 gggtgcggtg tcggaatcgg tagaggtgct gctcccgtcg ccgacgatac gaagtcgccg
     1261 acgaggctgg atcgtcgccg tagtcatcgt cgtcgtgcta gggctgggcg ggggaggttt
     1321 cgctgcatac agatggctca ccgggggaca cctgttcgtc ggacccggcg cctcggagtc
     1381 cctcagcctg caggccggcc acaccatcta cgtcgggcta gagccgctgc ctgctgaccc
     1441 gaaccccaac gcaaccatcc gggttgacct tcgacgcgtc catccgctcg tgaggaccaa
     1501 cacctccgac gcgaccgtga ccgcagtaga agtcgccggc gcgcaggtca cctaccgcgt
     1561 cggtatccgc ggcggtaacc aagacatcgg cgccgtcgac ctcaccgtaa acactccctg
     1621 accaggcgaa caggcacccg ctcaccatcg cggcatcacg ggctggatca ggacgccatg
     1681 ctggcgcacg tgagactctg gagagttgcc gtggcgatct cgcttcccgc ggcgctggtg
     1741 gccggtacgt cgcacacctc ccgggctggc gagggcagtg cgccggcgcc gagcgtgagc
     1801 ccgacccttt cagccaccgt ctcagcccag tcgacaaccg ctgggtcgtc cgcacggccg
     1861 acgtcgcctt cgtcgacaga cattctgggc gcgtccgttt ggaacagtca cgtccttgtc
     1921 atcactggcc agtcactcgg cggcgtgacc agaggaatgt cgagcgctgc ggcggcccac
     1981 gcggccggcg ttgctgcctt caccgaagtc ggggacggcg tgctccaacc ctcgggaccg
     2041 ttgggtagct cgcagccccg cctctatttg agcaacttcg ccggtcagcc cgccgcgtcc
     2101 gtgggcggcg acttctcgtg cgtcgaagca tcggggtcag cgtccgggac atcgtcggtg
     2161 gtcaccaccc gcggaccgcg cctgggcgat ccgggaaccc gcgtgcgcga aatctacggc
     2221 gcggccgcca acttcatccc gatgcccacc accggcggca tcgaccccaa tcccggttac
     2281 gtcgttcacc aaggacagtt cgacctcgtc ttcaagctcg acgcgtccga atcgcacgtc
     2341 atcgcgatcg ctggaggcct cgcgccgatg acgcccagcg aatgcaacgg gtgagtgccg
     2401 caccggcgta ggagacgaag ctagctgggc gggtacgtcg agcgtcgcgt gcgcgggcct
     2461 gctcgcaaca cctgcgatgg caactcacgg cccacgatgc gttctgcttg cgcggccacc
     2521 tcgatgagga cctcgagatc gatgcctgtc gcaacgccca tgtcctcgac catgtacacc
     2581 aactcctcgg tcgccagatt gccagaagcc cctggtgcgt aaggacatcc gcccagacca
     2641 ccgacgctcg catcgaagtc ggcgacgccg agctgcaacg ccgctagaac gttcgccagc
     2701 gcagtcccgc gcgtgttgtg gaagtgcaaa ttcaacggga cgtcgggtac cgccatccgc
     2761 atcgagccga cgagctccgt gacccgcgtc ggcgtcgcca tgccggtggt gtccccgaac
     2821 gaaagcccgt cggcaccgtc cgccactgcc cgcgaagcca ctgacacgac gcggctcacc
     2881 gggacctccc cctcgtaggg gcagccccac gccgtcgcta ccgtcacgtg acagaccgca
     2941 tcctgctcat gcgcaaggga aattacccgc gcgatgtcat cgagcgactc ggccgtcgag
     3001 cgattcacgt tggctctgtt gtgggtatct gatgccgaca caacgatctc gatcacacga
     3061 catccggcgt ccaacgctcg caccgcccca cgcagattcg gcaccaacgc cgaataggta
     3121 atcgacggca cgtgctcgat cgacgcccat acgtcatcag cgtcggccat ctgcggaatc
     3181 gcccgtggca acacgaaact caccgtctcg atgcggcgca caccagtccg cgatagagcg
     3241 tcgaccaact ccaccttcgc ggcagtcggc accggcgctt cgttctgcag accgtcgcgg
     3301 gggccgacct cgcgaaggct gatgcgcgta gggagatcgc tcattgcccc gaccaccgag
     3361 gcgcccgctt ctcggtaaac gcgctgatcc cctcacgccg gtccgcggac atcgcagcgg
     3421 cccgccacgc ggcgtcctcg atttcgagac cctcgtgcaa cggggtaccg aacccagtac
     3481 gcattgcgtg cttcgcatgg cggacggcga ggggtgagtt cccctcgatc gtggccgcga
     3541 tatccaaggc caactgcctg acgtgcacat ccacggtgag ccggtcgacg atgccgagcc
     3601 ggaacgcctc ctgcgcgccg accttgcgac cggtaagcac cagatcggcg gcgcggccgg
     3661 caccgatacg acgggccagc aattgggtgc cgccaccgcc cggcaccaag ccgacggtca
     3721 cttcggtcag gccgaacacc gcgctctcgt ccgccacgat caggtcacac gacaacgcga
     3781 gctcgaagcc gccacccaac gcaaagcccc ccaccgcggc aatcaccggc atcggcaact
     3841 cgagcagcga accgaacatc gccctgatga acgggcgttg cgcgaggagt tgctcgttgc
     3901 tgaagcccgc gcgctccttc agatccgcgc caacgcagaa ggctcgctcc tgagacgacg
     3961 acagcacgac tgcgcgaacg gccgcgtccg cagccagagc gcgcagcacg ccaccgaggt
     4021 gtgcggcaag ctcggtcgac accgcgttca acgcgcccgg tcggtcgagg atgagctcga
     4081 cgacgtgttc gctgggtcgc cacatcaaga cgccagactg tccggcatcg ggcgacggaa
     4141 catcgctcat gcgatcgagg gtacgccgca gttcagcctt gctccgacgc gtcgtcgccg
     4201 gcagtcgggt gagtacccgc gggcccgcca cgtccggccc gaccgccgtc cgccacgctc
     4261 atcggaccgg accgaccgag gttctggtcg accatgcggt acacctcccg ctgcacgtgc
     4321 tcgaccttcg gcacatccgt caacgagatc tgcccgttgt cacctgccga ctcgacgacc
     4381 agggtgccgc ttcgcaacat gcgctcgagc accgtctcgg agaacgacac gtcgttgatc
     4441 cggttcagcg ggatgtcgcg accgtggcgc gcgaggatgc cgttgcgaat caccagccgg
     4501 tcggtggtca gcacgtactt cgtcgtcaac cagcgcaggt acggcaggat cgtcagccag
     4561 atcaccacca cggcggccac gccgatcagg atccatcgct cgatctgctg gccaccgccc
     4621 ttcgggatca gcgcgacgcc gaacccggcc agcaggcagg cgaccaagag caccagaacc
     4681 gggacgaaaa gccggcccca atgcgggtgc aggtcgagca caacttcttc gccgtccgac
     4741 agattcatgc ccatgccccg catcgttcca tgtcggcggg aattgcgctc gccatctcac
     4801 gcgagggcct gcaaaatctc cccatggctg acgattcgac ggtccgggcg gcgacgaccg
     4861 ccgacgccgc tgctatcgcg ggcatctacc ggccctacgt gctcgagacc gtcatctcct
     4921 tcgaggaaac cccgcctact gctgccgact tcgaggcacg catgctcgcg acgccgcgcc
     4981 tgccgtggct cgtcgccgtc cgggccgaca ccgccgtggg ctactgctac gcctcacgcc
     5041 accacacgcg ggcctcctac cgctggtcgg ccgacgtgtc cgtctacgtc gcgcagagtg
     5101 agcgcggccg cggggccggc cggctgctct acagcgagtt gctgccgctc gtgcgagcgc
     5161 tcggctacgt cagcgtgttc gccggcatca ccctcccgaa cgacgcgagc gtgggctttc
     5221 acgaggcgtt cggactcact ccggttggcg tctatcgaaa cgtcggcttc aagcacaacg
     5281 catggcggga cgtcgggtgg tggcagctca cgttcaccca accgccggac gaaccggccg
     5341 agccaaaacc gtggttgccg ccggacgtca cctgacgtgc accacgtcgc cggcgctgac
     5401 tgatcggcgt ccggtcgacg tctcgacgac cagcgagccg tcgacgttga tatccagcgc
     5461 gcgaccctgc aacgactcgc cgccgaggtc aacgctgacg tcgcgaccca gcgtcacgca
     5521 cagctcgcgg tagcggggca gcacactgcg ctcgccatcg ccatcgaccc gccgccacgc
     5581 aaggtactcg tcgccgatgc cgcgcaggat tgcccgcagc agtgtgtcgc gatccgtaca
     5641 actcgcgccg gccagcgcga gagaaatcgc tgtaggcact ggcaattcgg cgtcgcgtag
     5701 cgtgacattg agtcccacgc cgacgaccac cgcgtcgtcg acgacctcgg cgagaatccc
     5761 ggcgagcttg cgg
//
LOCUS       DATCBJ010000565         3145 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_457342, whole genome shotgun sequence.
ACCESSION   DATCBJ010000565 DATCBJ010000000
VERSION     DATCBJ010000565.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 3145)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3145)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3145
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_457342"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..516)
                     /locus_tag="VII50_11370"
     CDS             complement(<1..516)
                     /locus_tag="VII50_11370"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5051493.1"
                     /translation="MAWEYYLVVMLVYLGTDLLAVWGLNLEFGVAGIANLAYIVVVAA
                     GAYAYAVVTLGPSSNLGGFQTYIGGFTLPFPLPLLVGAAAGAVVGTLIGLTGLKRLRQ
                     DYQGVAMLVVSLTVVTVVSADTGLFNGTAGLALIPNPFAGLDPQTARWAYVGLVAVCC
                     LIGFVVLRRFTT"
     gene            complement(519..1409)
                     /locus_tag="VII50_11375"
     CDS             complement(519..1409)
                     /locus_tag="VII50_11375"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEY5051494.1"
                     /translation="MQLIIESIGFGIATGAVIALGAVGFSVQFGISNILNITYGALMT
                     LSAYIGLVLINHGINAWVALAISAVAVGAVSVIYHRILLAPLVRRGTRAIAMIIVTVS
                     AGTILQYVIVAIAGPSSQSYGEQSGETFHFLDLTLSFGQVTIIVLTAVLMLALHLLLT
                     RTTLGKAMRATSSNRTLARSCGIATSRISDIAWFISGCLCGAAGVALAITTVSFDFSL
                     GALFLIPMIAAAVLGGIGQPYGAMLGGLIIGVSSEVAAAVSNPNYKDVVAFVILIIVL
                     LVRPRGLFGAAWSSRRLMEG"
     gene            complement(1504..2805)
                     /locus_tag="VII50_11380"
     CDS             complement(1504..2805)
                     /locus_tag="VII50_11380"
                     /inference="COORDINATES: protein motif:HMM:NF013275.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY5051495.1"
                     /translation="MGSQRIWRVSVLVGATALVAACGSSSPSSSSSVPTQSGDITAGV
                     IAPFTGPAAQFGKLLSAPCFAATDLINKAGGILGKTISCSAIDDTGDPADAVPNVTKA
                     IATTTNLDMALGLESNTAATTVPLVNDAKIPIITTNGLVAFTKNTYPYYWRLTPSDDA
                     NGAAFAVWAVQKGFKNVAVVFQNGIGSEGNLPGLTSAMPKLGGSLGLQLTIPADAASY
                     SSEVARVINAHPDALIWSADPQTTATFMSEYKQLNNGVIPPMVTATDSLTPDFFDALK
                     KVVGVGYVTQDVYLVGSSFSQTTPAFATYKAALQADSKTSAIADVLSAVGPPASEYDG
                     INILALAMVMAKSTVGSYYNQFVTKVISAKPGAVVVSNFADGVAALKAGKQIQYVGVL
                     GAVVFNQYHNSAGQFAANVFNPDGSASRVGSISGTQVLGLL"
BASE COUNT          610 a          990 c         1033 g          512 t
ORIGIN      
        1 ggtggtgaaa cggcgcagca cgacaaagcc gatcaggcag cacacggcca ccaaaccgac
       61 atatgcccag cgagcggtct gcgggtcgag accggcaaag ggattgggaa tcagcgcgag
      121 gccggcggtg ccgttgaaca agccggtgtc cgcactgacg accgttacca cagtgagtga
      181 cacgaccagc atcgccactc cctggtagtc ctggcggagc cgtttgagcc cggtgagccc
      241 gatcaacgtc ccgaccaccg cacccgctgc tgcgccgacc agcagcggca gtggaaaggg
      301 cagcgtgaat ccgccgatgt aggtctggaa tcctccgagg ttcgacgacg gtccgagcgt
      361 caccaccgcg tacgcgtatg cgccggccgc gaccacgacg atgtacgcga ggttggcgat
      421 tcccgccacg ccgaattcga ggttgagccc ccagaccgcg agcaggtccg tgccgaggta
      481 gacgagcatg acgacgaggt agtactccca ggccacccct atccctccat caggcggcga
      541 ctcgaccagg cagccccgaa gaggccgcgc ggccggacca ggagcacgat gatgaggatc
      601 acgaacgcga cgacatcctt gtagttcggg ttgctcactg cagcggccac ctcactggag
      661 acgccgatga tgagtccacc cagcatggcg ccgtatggct gaccgatgcc gccgagcacc
      721 gcggcggcga tcatcgggat caggaacaac gcgccgagtg agaagtcgaa gctcaccgtg
      781 gtgatcgcca gcgcgacacc ggccgcaccg cagaggcacc ctgagatgaa ccaggcaatg
      841 tcgctgatgc gactggtcgc gatcccgcag ctgcgcgcca gggtgcggtt cgacgaggtc
      901 gcccgcatgg ccttgccgag cgtcgttcgg gtgaggagca ggtgcagtgc cagcatcaac
      961 accgcggtca gcacgatgat cgtgacctgg ccgaacgaca gcgtgagatc gaggaagtgg
     1021 aaggtctcgc cgctctgctc gccatagctc tgtgaggacg ggcctgcgat agccacgatc
     1081 acatactgga ggatcgtccc cgcggagacc gtgacgatga tcatggcgat cgcccgggtg
     1141 ccgcgacgca ccagcggagc caggaggatg cggtgatata tcacggagac agctccgaca
     1201 gcgactgcgc tgatcgcaag cgctacccac gcgttgatgc cgtgattgat cagcacgagg
     1261 ccgatgtacg cggagagtgt catgagcgcg ccgtaggtga tgttgaggat gttgctgatc
     1321 ccaaactgca ccgagaagcc gaccgcgccg agcgcgatca ccgcaccggt cgcgataccg
     1381 aaaccgatgg actcaatgat cagctgcaac ggtcacctcc actcgacgtg cgaaacggag
     1441 ctgccccgcc cgtcgcgggt gcgactccgt ttcgattgta ggaatgaggc actgcctcag
     1501 ccgtcacagc agaccgagca cctgcgtgcc ggagatgctg ccgacccggc tggcgctacc
     1561 gtcagggttg aagacgttcg cagcgaactg tccggcggag ttgtggtact ggttgaacac
     1621 caccgcaccg aggacgccaa cgtactgaat ctgcttgccg gccttcagcg ccgccacgcc
     1681 gtcagcgaaa ttgctcacca cgaccgcccc cggcttcgcg gagatgacct tggtcacgaa
     1741 ctggttgtag tacgaaccca cggtcgactt ggccatgacc atggcgaggg caaggatgtt
     1801 gatgccgtcg tactcggacg ccggcggtcc cacggcgctc aggacgtccg caatcgcgct
     1861 cgtcttggaa tccgcctgga gcgcggcctt gtaggtcgcg aatgcagggg tggtctggct
     1921 gaacgacgac ccgaccaggt agacatcctg ggtcacatag ccgacaccga caaccttctt
     1981 gagcgcgtcg aagaagtccg gcgtcagcga gtcggtcgcg gtcaccatcg ggggaatcac
     2041 accgttgttg agctgcttgt actccgacat gaacgttgcc gtggtctgcg gatcagccga
     2101 ccagatcagg gcatcggggt gggcgttgat gacccgggcc acctcgctcg agtatgaggc
     2161 ggcgtccgcg ggaatggtca gctgcagacc aagactgccg ccgagcttcg gcatcgccga
     2221 tgtcaggccc ggaaggttgc cctcggaccc gatcccgttc tggaagacga ccgcaacgtt
     2281 tttgaagccc ttctgcacgg cccacaccgc aaacgccgcg ccgttggcgt cgtccgatgg
     2341 ggtgagccgc cagtagtacg gatacgtgtt cttggtgaag gccacgagcc cgttggtcgt
     2401 gatgatcggg atcttggcat cgttgacgag cggcaccgtg gtcgccgccg tgttgctctc
     2461 gaggccgaga gccatgtcca ggttggttgt ggtcgcgatc gccttggtca cattcgggac
     2521 ggcgtcagcg gggtcacccg tgtcgtcgat cgccgagcaa ctgatcgtct tgccgaggat
     2581 gcccccggct ttgttgatca agtccgtggc ggcgaagcac ggggccgaca gcagcttccc
     2641 gaactgggct gcgggtccag tgaagggcgc gatgacgccc gcggtgatgt cgcctgattg
     2701 cgtcggtacg gacgacgagg aggacggact actgctgccg cacgcagcga cgagtgcggt
     2761 cgctcccacc agaacggaca ctcgccagat tctctgcgag cccatgggaa aacctccttg
     2821 tgaaggtcac gccttccggc ggtgtcacgt ccacgccgtc caccctaccg tggtgagcag
     2881 taccataatg gacgcggcag acaagtgcaa ggcccgccgg cctttgcgcg tcgcgatgcg
     2941 gcaacccgac acggaacggg cgaaggagac gcgatgatcg acgacggttt cgagatcaaa
     3001 tttctccccg gaattcctcc ggaggaggcg gggtgtcctc cgctctcgcc gcgcacggag
     3061 cgcgtcgacg gaatgatcat cgagtacgac acggccgttc gcatgcgcga cggtatcgag
     3121 atctatgccg acgtgttccg gcccg
//
LOCUS       DATCBJ010000566         3453 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_68242, whole genome shotgun sequence.
ACCESSION   DATCBJ010000566 DATCBJ010000000
VERSION     DATCBJ010000566.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 3453)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3453)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3453
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_68242"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..744)
                     /gene="ftsX"
                     /locus_tag="VII50_11385"
     CDS             complement(<1..744)
                     /gene="ftsX"
                     /locus_tag="VII50_11385"
                     /inference="COORDINATES: protein motif:HMM:NF038346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HEY5051496.1"
                     /translation="MRFRFVFSELAIGLRKNVLMMFSVVVITAFSMMALGVALLVQRQ
                     VGHMKHYWYNQLQVSVYLCSAHSTNPNCPTAATQTQIADVQSQLEALQPTVKSIDFVD
                     QARAYELFKETFKDAPDLVKNTSPDALPESFVVKLQDPKKFDVVSSALSGKPGVDSVQ
                     DEHAFLNKLFAILNQLRVVAIVISATVIIAAVVLIGVAVQVSAASRKRETGIMRLVGA
                     SNLYIQLPFLLEGIVGGLLGAVVGFGALSA"
     gene            complement(775..1464)
                     /gene="ftsE"
                     /locus_tag="VII50_11390"
     CDS             complement(775..1464)
                     /gene="ftsE"
                     /locus_tag="VII50_11390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006349025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HEY5051497.1"
                     /translation="MIRFDDVTKVYPKQSRPALDNVTLEVEKGEFVFLVGASGSGKST
                     FLRLVLKEEKPSHGRVHVAGKDLGRLSSWKVPGLRRQIGTVFQDFRLLPNKTVTQNVA
                     FALEVIGRPQTQIRKIIPEVLDLVGLADKANRLPGELSGGEQQRVAIARAFANRPIIL
                     LADEPTGNLDPTTSVGIMKLLDRINRTGTTVVMATHDSQIVDSMRRRVIELEGGKMIR
                     DQSRGVYGYSN"
     gene            1650..1832
                     /locus_tag="VII50_11395"
     CDS             1650..1832
                     /locus_tag="VII50_11395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5051498.1"
                     /translation="MSCEHLICAACAGPVDEGRCPSCRAARAHLHPHGGTAFPPAAIV
                     AIVAMVALILLLLSGH"
     gene            complement(1887..3035)
                     /locus_tag="VII50_11400"
     CDS             complement(1887..3035)
                     /locus_tag="VII50_11400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5051499.1"
                     /translation="MSVGVGGVASLALVTAASAHASLLSGTAQCQSNGTYTVTWTISN
                     DWILADSITDVGHTGGGTLTGLPTTIPASAAKTPPFESVTVLQTGVSGSATSASLTVH
                     GTWTDKVTETNSGSVALPGTCSRGTAATAPTFTNATCSTATGSYTIPSSTVADYFVAI
                     GNGAPVAAPAGTVSEAVGTLIKITAKAKSGGPALTGTTAWSATIAGAGSCGGGGSSSP
                     PPPPPPPPGSNTVTPAVPSITQGVCLSGKESAAFYTIPGITGVTYLNGTTGVAAGNHT
                     VAYGSSTTITAVALTGFAFPSGATTTWTLAANKAATCAAAPTSVLGVTFTQPPPKKPP
                     VAVLPFTGQPILQTTLVAFGLLLAGGLLIGSSRRHRLSQLTWIDRAGK"
BASE COUNT          634 a         1104 c         1127 g          588 t
ORIGIN      
        1 cgccgatagg gcaccgaatc cgaccaccgc gccgagcaat ccgccgacga tcccctcaag
       61 caggaacggc aactggatgt acaggttcga tgcaccgaca agccgcatga tgccggtctc
      121 gcgcttacga cttgcagcag agacctgtac cgccacaccg atcaagacga cggccgcaat
      181 gatgaccgtc gccgaaatga cgatggccac aacacgcagt tgattgagga tcgcgaatag
      241 cttgttgagg aacgcatgct cgtcttgcac cgaatcgacg cccggtttcc ctgacaacgc
      301 actgctcacg acatcgaact tcttcgggtc ttgcaacttc acgacgaacg attccggcaa
      361 cgcatcgggc gacgtgttct tcaccaggtc aggggcgtct ttgaacgtct ccttgaagag
      421 ctcgtaagcc cttgcctgat cgacaaaatc aatgctcttc actgtcggtt gcagcgcttc
      481 gagctggctc tgaacgtcgg cgatctgggt ctgcgtcgcc gcggtcgggc agttcggatt
      541 cgtgctgtgc gccgagcaca aatagaccga gacctgcagc tggttgtacc agtagtgctt
      601 catgtggccg acctgccgct gcacgagcag ggcgacgccg agcgccatca tcgagaacgc
      661 ggtgatgaca acgacggaga acatcatgag gacgttcttg cgcaggccaa tagccagctc
      721 tgagaacacg aatcgaaacc gcatgaggtc cttaccggcc agtagcgaac gggtctagtt
      781 ggagtagccg tacaccccgc gggactgatc gcggatcatc ttgccgccct cgagctcgat
      841 cactcgccgc cgcatcgagt caacgatctg cgagtcgtgc gtggccatca cgacggtcgt
      901 accggtccgg ttgatccggt cgaggagctt catgatgccg acgctcgtcg tgggatcgag
      961 gttcccggtg ggctcgtcgg caagcaggat gatgggtcgg ttggcgaagg cacgcgcgat
     1021 cgcgacccgc tgctgctccc cgcccgacag ctcccccggc agccggttgg ccttgtcggc
     1081 caggccgacg aggtcgagca cctcggggat gatcttgcga atctgggtct gcggccggcc
     1141 gatcacttcg agtgcgaagg cgacgttctg cgtcaccgtc ttgttcggca gcagccggaa
     1201 gtcctggaag acggtaccga tctggcgccg caagcccggg accttccacg acgacagccg
     1261 gccgaggtcc ttacccgcca cgtgaacccg accgtggctc ggcttctcct ccttgagaac
     1321 cagccgaagg aaggtcgatt tgcccgatcc ggaggccccg acgaggaaga caaattcgcc
     1381 cttttcgacc tcaagggtca cgttgtcgag ggctggccgc gactgtttgg ggtagacctt
     1441 ggtcacgtcg tcgaacctga tcacgacatc atcacgcctg ccagtctagg cacgaaggag
     1501 ccggacgccg gggagcggcc gcgcaaggtc ggcccggatt ctgccgaatg agggagaacg
     1561 aaatgcatcc gatggctcgc gcagcggcga aaccggaggg acactagtgg caagacctcc
     1621 gggtcgaacc gagaagggag gcgagcgcga tgtcatgtga gcacctcatc tgcgccgcct
     1681 gcgcaggacc cgtcgacgaa ggccgctgcc ccagctgccg cgccgcccgc gcccacctgc
     1741 acccccacgg cggtacggcg ttcccgccgg ccgccatcgt ggcgatcgtg gcgatggtcg
     1801 cgctgatttt gctgctgctc tccggccact aacacgacaa tgaagaaagg cccggcgatt
     1861 tctcgccggg ccctcctttt gagtccctac ttgccggccc ggtcgatcca ggtcagctgg
     1921 ctgagccggt gccgacgact cgaaccgatc aacaagccgc cagcgagcaa caacccgaag
     1981 gcgaccaggg tcgtctggag gatcggctgc ccggtgaacg ggaggaccgc caccggtggc
     2041 ttcttcggcg gcggctgggt gaaggtcaca cccaggacgg aggtcggtgc cgcggcgcag
     2101 gtggctgcct tgttagccgc cagcgtccac gtcgtggtcg ccccgctcgg gaacgcgaaa
     2161 cccgtgaggg ccaccgccgt gatcgtggtg ctcgacccgt acgccaccgt gtggttgccg
     2221 gcggcgactc cggtcgtgcc gttgaggtag gtcacgccgg tgatacccgg gatcgtgtag
     2281 aacgctgcgg actccttgcc gctcaggcac acgccctgcg tgatgctcgg cacagcaggc
     2341 gtgacggtgt tcgacccggg cggcggcggc ggaggcggcg gcgggctcga actgccgcct
     2401 ccaccacaac taccagcgcc cgcgatggtc gccgaccacg ccgtggtgcc ggtgagcgcc
     2461 ggaccgccgg acttggcctt tgcggtgatc ttgatcaggg tgccgaccgc ttcactcacg
     2521 gtgcctgcgg gtgcggccac aggtgcgccg ttaccgatcg cgacgaagta gtcggcgacg
     2581 gtcgacgacg gaatcgtgta gctgcccgta gccgtcgaac aggttgcgtt cgtgaaggtc
     2641 ggtgcggtcg cggccgttcc gcgcgagcag gtgccgggca acgcgaccga gccactgttg
     2701 gtctcggtga ccttgtcggt ccaggtgccg tgcacagtca acgacgcgct tgttgcgctg
     2761 ccggacacgc ccgtctgaag gaccgtgacg gactcgaacg gcggcgtctt ggcggccgac
     2821 gcgggaatcg tggtcggcag gcccgtgagg gtgccgccac cggtgtggcc gacgtcggtg
     2881 atcgagtcag cgaggatcca gtcgttggag atcgtccagg taaccgtgta ggtgccattg
     2941 gactggcact gggcggtacc ggagagaagg ctcgcgtgcg cgctagcggc ggtgacgaga
     3001 gccagcgagg ctaccccacc gacgccgacg ctcaaggcga gtgccgcaat cgaacgcgga
     3061 cgtgggagcc gcggaattcg cggatgtaac cgcataacag acctgccctc tagtcgaatg
     3121 acaaggggtc ggtctcacca ctcgctcccc gcaaccatcc gtaaaagatc tacggcaggt
     3181 gaccggtgtt accagtcgcg gatcaaagcg tccccacgtt gttgcttccc caatatggcc
     3241 gtacacgcaa cggtttcgca atccaactgg ccgatcatgg ggtggaattc gtcgaagtta
     3301 cgccagccgg actagccttg ctagcccgtc cggagcagcg aattcgcggc aagaaagcgc
     3361 cctggcggat caacagatcg cccgcccgtc ggcgtccgac tgactcgctt gaggcttgaa
     3421 tcggtcgaaa cgcgcccaga atgagctaac ctg
//
LOCUS       DATCBJ010000567         3679 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_60090, whole genome shotgun sequence.
ACCESSION   DATCBJ010000567 DATCBJ010000000
VERSION     DATCBJ010000567.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 3679)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3679)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3679
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_60090"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..996)
                     /locus_tag="VII50_11405"
     CDS             complement(<1..996)
                     /locus_tag="VII50_11405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HEY5051500.1"
                     /translation="MTGQLLDALSPDTFSPERRSPGAAGSRADAVALGARLMANVEHV
                     VHGRRPAVELVVAGLLSGGHVLVQDVPGSGKTTLARSVARSIGGTFRRIQGTADLLPS
                     DITGSSMWDPARHEFIFVPGPIFTNVLLVDELNRATPRAQSALLEAMDESTVTVDGIR
                     HSLPEPFFVVATQNPTDQYGTFPLPEGQLDRFAVALSLGPNDAATERRVMREQLLRAT
                     VDELQPVLEADELRWIQLEVRSTHIADPVLDFALAVLDATRSHDSLRVGASSRAGLSL
                     MRCAQAHALLSGRDYVVPDDVKILAPSVLGHRLVLADGIAGERGTHHGMGVGVGAE"
     gene            complement(1309..1632)
                     /locus_tag="VII50_11410"
     CDS             complement(1309..1632)
                     /locus_tag="VII50_11410"
                     /inference="COORDINATES: protein motif:HMM:TIGR00377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HEY5051501.1"
                     /translation="MPAFSVCGDLDVSTAANVRAELTDLLSVAGTDDLIVDLEHMGSL
                     DTVGLGLLVGIHRQAQRGGRRLVLTRVPPRMMRVLAVTRLRRVLAIDEGGAELVGAVD
                     ARQGA"
     gene            complement(1816..2688)
                     /locus_tag="VII50_11415"
     CDS             complement(1816..2688)
                     /locus_tag="VII50_11415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase family protein"
                     /protein_id="HEY5051502.1"
                     /translation="MTATTGRLAVIGAGLMGSGIAQVAAQAGYDVVLHDTTSEALERA
                     MTAIAASQLRFVAKKSLTEADAEAARARIATTTDLGDIVGAQIVVEAVFEQLEVKRQI
                     FSELDRLADPDAILATNTTAIPITKIAAATRRPERVVGTHFFSPVPMMALCELVRGDE
                     TSDDTLARARAFAEALGKTCVVVERDIAGFVTSRLLIAFVNEAIALVEGGVATPADID
                     TACRLGFGHVMGPLATVDLTGLDVILHAARAIYAETGDEKFAPPALLERMVEAGALGR
                     KTGHGFYDYEGRPA"
     gene            complement(2685..>3679)
                     /gene="murA"
                     /locus_tag="VII50_11420"
     CDS             complement(2685..>3679)
                     /gene="murA"
                     /locus_tag="VII50_11420"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020657302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HEY5051503.1"
                     /translation="VLGPLLARCGEAHVALPGGDNIGSRGLDLHVAGLQKLGAELESE
                     HGFLIARAPNGLVGAPVWLDFPSVGATENLMMAGVLARGTTIIDNAAREPEIVDLARM
                     LQGMGAKVDGAGSSTIEVQGVTALSPTSHATVPDRIVAGTFAMAAVMTRGDVEIHNAR
                     PEHLEIVLDRLVGCGAAASELDDGFHVEMNGRASAVDVVTLPYPGFPTDFLPMIIALN
                     SVAEGAAMVTENVFEGRFRFIQELVRLGADIRTDGHHAVIRGRERLSGAPVEATDIRA
                     GAGLVLAGLVADGVTTVSELHHIDRGYADFVQQLQALGADVVREPDPSTMAFDQ"
BASE COUNT          644 a         1256 c         1187 g          592 t
ORIGIN      
        1 ctccgcgccc accccgacac ccatcccgtg gtgcgtgcca cgctccccgg cgatgccgtc
       61 ggccaggacg agtcgatgcc ccagcacgct gggtgcaagg atcttgacgt cgtcgggcac
      121 cacgtagtcg cggccggaca gcaatgcgtg tgcctgcgcg cagcgcatga gagaaagccc
      181 ggcgcgcgaa ctcgcgccga cccgaaggct gtcgtgcgac cgggtggcgt cgagcaccgc
      241 caacgcgaaa tcgagcaccg ggtcagcaat gtgcgtactg cgtacctcaa gctgaatcca
      301 gcgcaactca tccgcctcca gcactggctg aagttcatcg accgtggccc gcagcaactg
      361 ctcccgcatt acccggcgtt cggttgcggc gtcgttcggt ccaaggctca acgcaacagc
      421 aaaacggtcg agttgtcctt ctggcaaagg aaaagttccg tattggtcgg tcgggttctg
      481 ggtcgccacg acgaagaatg gctcgggcaa cgaatgccgg atcccgtcga cggtgacggt
      541 cgactcatcc atcgcttcga gcaaggccga ctgggcgcgg ggggtcgcgc gattcagctc
      601 gtcgacgagc agcacgttgg tgaagatcgg acccggcacg aagatgaact cgtgacgcgc
      661 cggatcccac attgacgatc cggtgatgtc actcggcagc aggtccgccg tgccttgaat
      721 acgccggaaa gtgccaccga tagaacgcgc gacgctgcgg gcgagcgtgg tcttcccact
      781 gcccggaaca tcctgcacga gcacatgacc gccggagagc aagccggcga caacgagttc
      841 gacagccgga cgccgtccgt gcacgacgtg ctcgacgttg gccatcaacc gcgcgccgag
      901 cgccaccgcg tcagctcggg atccggccgc gccgggcgac ctgcgctcag gcgagaacgt
      961 gtcgggcgac agcgcatcaa gcagctggcc ggtcatggcg tcctccggtc ggtcgggtga
     1021 gtcccaaccc catcggccgc gcctagtcag ttatgaggca gttatggagc cgatcggcag
     1081 cggccactcg actcgccaaa tctgccgagc gctcactggc cagctgtgga taacgactct
     1141 tctccgaacg ctttgctctg cagctatacc actgcagaac agctctgcag ctataccact
     1201 gcagagcaaa gcgcccgaaa gagtgttccc ccaggagggg agcgccgagg aatcggtcaa
     1261 atcgacgcgg gcaggcggtg gctgcgctcg ggctagtcgg gctgcgggct aggcgccttg
     1321 acgcgcgtca accgcgccca ccagctccgc gccaccttcg tcgatcgcga gcacccggcg
     1381 gagccgggtg acggccagca cccgcatcat gcggggcggg acccgggtca gcaccaatcg
     1441 gcgtccgcct cgctgggctt ggcggtgaat tccgacaagg aggccgagac cgaccgtatc
     1501 gagggagccc atgtgctcga gatcgacgat cagatcgtcg gtccccgcga cgctcagaag
     1561 gtcggtcagc tcggctcgca cattcgcggc cgtggagacg tcaaggtccc cgcacacgct
     1621 gaaagctggc atcggatccc cctcgtttgg tcccgtcacc gtctatgacg cggtgacgag
     1681 ccccctggtt gcgtcagaag cgtcacaaat ccgccacgat tcgcccagcc gacacgccgt
     1741 tagaggccta actgtgatgt gacggatgtg acgggtgagc catgctggac gcccgagccc
     1801 ggcaacgcgg tcgccctacg caggccggcc ctcgtagtcg tagaagccgt gaccggtctt
     1861 gcggccgagc gcgccggcct cgaccatccg ttccagcagg gcgggagggg cgaacttctc
     1921 gtcgccggtc tccgcgtaga tggcgcgcgc cgcgtgcagg atcacgtcga gtccggtcag
     1981 gtcaaccgtc gcgagcgggc ccatcacgtg cccgaagccg agtcggcacg cagtatcgat
     2041 gtctgccggc gtcgccaccc cgccttcgac gagcgcgatc gcctcgttga cgaacgcgat
     2101 cagcagccgc gaggtcacga atccggcaat gtcgcgttca acgaccacac acgtcttccc
     2161 cagcgcttcg gcaaatgcac gcgcgcgggc gagcgtgtcg tcggaggtct cgtcgccgcg
     2221 gacgagttcg cacagcgcca tcatcgggac cggcgagaag aagtgcgtgc cgacaacgcg
     2281 ctcgggtcgc cgcgttgccg ccgcgatctt cgtgatcgga attgcggtgg tgttcgtcgc
     2341 gaggatcgca tccgggtccg cgagtcggtc gagctcgctg aagatctgcc gcttcacctc
     2401 aagttgctcg aacactgcct cgacgacgat ctgcgcaccg acgatgtcgc cgagatccgt
     2461 cgtggtggcg atcctggcgc gagcagcttc cgcgtcggcc tcagtgaggc ttttcttcgc
     2521 gacgaaccgg agctgcgacg ccgcgatcgc cgtcatcgca cgttcgagag cttcggatgt
     2581 ggtgtcgtgc agcacgacgt catagcccgc ctgcgcggcc acctgcgcga ttcccgatcc
     2641 catcaatccc gcgccaatga ccgcgagccg ccctgtcgtc gccgtcactg gtcgaacgcc
     2701 atggtggatg gatcaggctc gcgaaccacg tccgcgccca acgcctgaag ctgctgcacg
     2761 aagtcggcgt agccgcgatc gatgtggtgc agctcactca ccgtcgtgac gccatcggcg
     2821 acgagaccgg ccagcacgag tccagcgccg gccctgatgt cggttgcctc gaccggagcg
     2881 ccagaaagcc tttcacggcc gcgaattacc gcgtggtgac cgtcggtgcg aatgtccgcg
     2941 ccgagtcgta ccaactcctg gatgaaacgg aagcggccct cgaacacatt ctccgtaacc
     3001 attgcggctc cctccgcgac ggagttcagc gcgatgatca tcggcaggaa gtcggtcggg
     3061 aacccgggat acggcagggt cacgacgtcc accgctgatg cgcgaccgtt catctcgacg
     3121 tggaagccgt cgtcgagttc gctcgcggcc gcgccacatc cgacaagccg gtcgagcacg
     3181 atctcaagat gctcgggtcg cgcgttgtgg atctcgacat cgccccgcgt catcactgcg
     3241 gccatcgcaa aggtgccggc cacgatgcga tcgggtaccg tcgcgtggct cgtcggcgag
     3301 agcgccgtca cgccctgaac ctcgattgtc gaactgccgg cgccatcgac cttcgcgccc
     3361 atgccctgca gcatccgggc taggtcaacg atctcgggtt cgcgcgccgc gttgtcgatg
     3421 atggtcgtgc cgcgcgcgag cactccggcc atcatcaggt tctcggtggc accgacactg
     3481 gggaagtcga gccacaccgg cgcgccgaca agtccgttcg gtgcgcgagc aatcaggaag
     3541 ccgtgctcgc tctcgagttc ggctccaagc ttttgcagcc cagccacgtg caggtcgagg
     3601 ccgcgcgagc caatgttgtc gccgccgggc agtgcgacgt gggcctcgcc gcacctggcc
     3661 agcagcggcc cgagtaccg
//
LOCUS       DATCBJ010000568         2339 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_422401, whole genome shotgun sequence.
ACCESSION   DATCBJ010000568 DATCBJ010000000
VERSION     DATCBJ010000568.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2339)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2339)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2339
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_422401"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..757
                     /locus_tag="VII50_11425"
     CDS             <1..757
                     /locus_tag="VII50_11425"
                     /inference="COORDINATES: protein motif:HMM:NF024867.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEY5051504.1"
                     /translation="ASAMDAIAEDAFLRAVELPRAGEGDVRAGYPWTLPAIAALRERL
                     VLDPAVTYLIGENGSGKSTLLEAIAVAAGMNAEGGSSNFRFSTRDSHSELGQSIRLVR
                     GARRPRTDFFLRAESLFTAATYIEALPDDPLRPYGGVSLHEQSHGESFLAVMINRFGS
                     DGLYLLDEPEAALSTQNCLTCLRRIHELVLARSQFIIATHSPIILAYPDAAIYSCQDG
                     GLKRIAYEDAEPVRLTADFLAARERFLHELFSG"
     gene            940..1449
                     /locus_tag="VII50_11430"
     CDS             940..1449
                     /locus_tag="VII50_11430"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY5051505.1"
                     /translation="MTDIVVRAGAEGDAAVILGLWDEAIAWLVARGQPEQWGTEPASA
                     RAKTRETVQQWVRGSGVRVAEDGGRPVGVSVIVPSPPDHVPAIARSETYLLFLVSSRA
                     CAGQGIGSALVRRAAADARNAGSDVLRVDCWAGAPALIAWYTRQGFVPSDTYTVHDGW
                     RGQVFEMDL"
     gene            1537..2058
                     /locus_tag="VII50_11435"
     CDS             1537..2058
                     /locus_tag="VII50_11435"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY5051506.1"
                     /translation="MSADTSVMFLREAQPADAGEVAAVHVRSWQVGYQALLPAAYLDT
                     LQPHDRARHCTFGDPDPLQPATIVAVEGDTICGFATTGPSRQDGDIGELLALYVDPDR
                     WGLGIGRSLLTDARARMAQRGFQHAILWVLVGNVRAERFYRIDGWTPNGAHRHDTVWS
                     LMVDEIGYGRSLT"
     gene            complement(2077..>2339)
                     /locus_tag="VII50_11440"
     CDS             complement(2077..>2339)
                     /locus_tag="VII50_11440"
                     /inference="COORDINATES: protein motif:HMM:NF014608.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HEY5051507.1"
                     /translation="FAMALSNMLAEAGTAPESVNVDATVALRLVDGAPTITKIDLAVV
                     GRVPGIDEAAFKEHAANAKAGCPVGRALAGVPEITLAASLDA"
BASE COUNT          362 a          802 c          772 g          403 t
ORIGIN      
        1 tgcctctgcg atggacgcga tcgcagagga cgcgtttcta cgcgcggtgg agctgccacg
       61 ggctggggag ggcgacgtgc gcgctggtta tccgtggact ctgcccgcga tcgccgcact
      121 gcgcgagcga ttggtgctcg atccggcggt cacttaccta atcggcgaga acggaagcgg
      181 gaagtccaca ctacttgagg cgatcgcagt cgctgccggg atgaacgccg agggcggctc
      241 ctcgaacttc cgattctcaa cgcgcgactc gcactccgaa ctcggccagt cgatccgact
      301 cgtgcgcggc gcgcgccgtc cgcgcaccga cttcttcctg cgcgccgaga gcctatttac
      361 ggcggccacc tacatcgagg ccctgcccga cgatccgctt cggccctacg ggggcgtttc
      421 actgcacgag caatcgcacg gcgagtcgtt cctggcggtg atgatcaatc gcttcggttc
      481 cgatggtctg tacctgctcg acgaaccaga ggcggcgttg tcgacgcaga actgcctcac
      541 ctgcctgcgc cggattcacg aactcgttct cgcacgctca cagttcatca tcgctacgca
      601 ctcaccgatc atcctggcct atcccgacgc cgcgatctac agctgtcagg acggcggcct
      661 caagcgcatc gcctatgaag acgccgagcc ggtccggctc accgccgact tcctcgcggc
      721 acgcgagcgc tttctgcacg aactcttctc cggctagaag ctccgcgatc cacgtcgatt
      781 ctctggcgcg gggcgccgcg gtcatctcgg cgccgcccgt cgtggatcac ctggcgcggc
      841 ctgttcccgc gcatggacga cgcgcgagct gcctggcccg gattccgcct ggttgtcgcc
      901 cgtcgggccg agcgacagca agtcgctacg gtcttggcga tgactgacat cgtggtccgt
      961 gcgggagccg agggggacgc tgcggtgatt ctgggcctct gggacgaggc gatcgcgtgg
     1021 ctcgttgcgc gtgggcagcc tgagcagtgg ggcaccgagc cggcatccgc tcgggccaaa
     1081 acccgcgaga cggttcagca gtgggtccgc ggctctggag tgagggttgc ggaggacggt
     1141 ggccggccgg tcggcgtcag cgtgatcgtg ccgtcgccgc ccgaccacgt gcccgcgatc
     1201 gcgcgaagcg agacgtatct cctcttcctc gtcagctcta gagcctgcgc ggggcagggc
     1261 atcggctcgg ctctggtccg acgcgctgca gcagacgcac gcaacgcagg ctcggacgtc
     1321 ctgcgggtcg attgctgggc gggagccccg gccctgatcg cgtggtacac gcgtcaaggc
     1381 ttcgtcccct cagacaccta caccgtgcac gacggctggc ggggccaagt cttcgagatg
     1441 gacctctgac caggggtcga tgttcgtaca cgctcgctgc tcctcgtcgc gaaggggagc
     1501 acggagtgga gtgcgttgag tggtccgaca gcaacggtga gcgcggatac ttcggtgatg
     1561 ttcctgcgtg aggcgcagcc ggccgatgcc ggcgaagtcg cggcggtgca tgttcgctcc
     1621 tggcaggttg gctaccaggc cctgctgccg gcggcctacc tcgacaccct ccaaccgcat
     1681 gaccgagccc ggcattgcac cttcggggac cccgaccccc ttcagccagc gacgatcgtg
     1741 gccgtcgagg gcgacacgat ctgcgggttc gcgaccactg gcccatcgcg acaagacggc
     1801 gatatcggcg agctcttagc gctctacgta gatcccgatc gctgggggct gggcatcggc
     1861 cgttcactcc tgaccgatgc ccgagcccgc atggcgcagc gcggcttcca gcacgcgatc
     1921 ctgtgggtgc tggtcggaaa cgtgcgagcc gaacgcttct accgaatcga cggctggacg
     1981 cccaatggcg ctcaccgcca cgacacggtc tggagcctca tggtcgacga gatcggctac
     2041 ggaaggtccc ttacgtagcc ccagttacct ctggggctac gcgtccagag acgctgccaa
     2101 cgttatctcg gggaccccag ccagagcgcg gcccaccgga cagccggcct tggcgttcgc
     2161 agcatgctcc ttgaaggccg cctcgtcgat gcccgggacg cgaccaacca cggcaaggtc
     2221 gattttcgtg atcgtcggag cgccgtcgac cagtcgcagc gccaccgtcg cgtcgacgtt
     2281 caccgactcg ggcgccgtgc cggcctcggc cagcatgttc gagagcgcca tcgcaaagc
//
LOCUS       DATCBJ010000569         2334 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_199939, whole genome shotgun sequence.
ACCESSION   DATCBJ010000569 DATCBJ010000000
VERSION     DATCBJ010000569.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2334)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2334)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2334
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_199939"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1246)
                     /locus_tag="VII50_11445"
     CDS             complement(<1..1246)
                     /locus_tag="VII50_11445"
                     /inference="COORDINATES: protein motif:HMM:NF005453.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaminase"
                     /protein_id="HEY5051508.1"
                     /translation="MDRSRLTSLIEGERAMYIATHPVAAAMNRSATHLVRRVPMTWMA
                     KWSGGFPLYLDGASGNRITDVDGHDYVDFALGDTGAMAGHSPRPTVEAVSERMRRGIT
                     AMLPTADAEWVGAELTRRFGVPRWSFTLTATDANRWAIRLARLATGRPKILIFAYCYH
                     GSVDEAFAVVGPNGDVVSRVGNVGAPVALGLTTRVAEFNDLASVESALAHGDVAAILT
                     EPALTNIGIVLPEPGFMAGLRELATRYGALLTIDETHTFSAGPGGATKLWDLQPDIFV
                     IGKSIGGGIPCGAYGLSDQVWQLVEAHPEADLVDVGGVGGTLAGNALSVAAMRATLEH
                     VLTDDAFGAMVNLATQFTRGVQETIDRTGVPWSVSQLGARAEYRFVSPAPRDGTSSAA
                     AGDDELDEYFHLSLLNRGVLITP"
     gene            complement(1266..2198)
                     /locus_tag="VII50_11450"
     CDS             complement(1266..2198)
                     /locus_tag="VII50_11450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5051509.1"
                     /translation="MAIEGAFSSAQTGLTVAQAPDGAIFVAGKVASPQVIWVVDGVQP
                     AGIAEHVQGPVTALAADAANLYVGVGQSVIAYSRTTGNVVRTWHTALPGTLTQLVVAG
                     TSVWGLSTQSNDNSTGAPDGLVELDPSSSSPARSVTGFTGAFSIAAGPAGVYYVTKQS
                     SQLVEQTNAGATITAPTNQQVNLQLSGPSAIQAIAVADGHVIVQHDAGQGLDAQLNTY
                     DATTLVGPTAQINFSAAEPIAVTASGLFVVGNPDTGACTADQTACVRRFALPAGDVGD
                     PLALPDNTIASTVTGPYPTVVLAVGSGLHVLRIS"
BASE COUNT          364 a          772 c          821 g          377 t
ORIGIN      
        1 aaggcgtgat gaggacgccc cggttcaaca agctgaggtg gaagtactcg tcgagctcgt
       61 cgtcgccggc agctgcggaa ctggtgccat cgcgtggggc gggtgagacg aatcgatact
      121 ccgcccgcgc gccgagctga ctgaccgacc acgggacgcc ggtgcgatca attgtttcct
      181 gcacaccgcg ggtgaactgt gtcgcgaggt tgaccatcgc gccgaacgcg tcgtcggtca
      241 gaacgtgctc gagggtcgct cgcatcgctg cgaccgacaa cgcattgccg gccagcgtgc
      301 cgccgacacc gccgacgtcg accaggtcgg cctctgggtg agcctcgacc agctgccaca
      361 cctgatcgct taggccgtag gcaccgcacg ggatgccgcc gccgatgctc ttgccgatga
      421 cgaagatgtc gggctgcagg tcccagagct tggttgcgcc tcccgggccg gccgagaagg
      481 tgtgcgtctc gtcgatcgtc agcagcgcgc cgtatctggt ggcgagctca cgcaggcccg
      541 ccatgaatcc cggctccgga agcacgatcc cgatgttcgt gagcgcgggc tcggtcagga
      601 tcgccgccac atcgccatgt gcgagcgccg actcgacgct ggcaaggtcg ttgaactcag
      661 cgaccctggt ggtgaggccg agggctactg gcgcgccgac gttgccgacg cgcgagacca
      721 cgtcgccgtt cgggccgacg acggcgaagg cttcgtcaac cgagccgtgg tagcagtacg
      781 cgaagatcaa gatcttcggt cggccggtcg ccagccgcgc gagcctgatt gcccaccggt
      841 tcgcgtcggt cgcggtcagt gtgaacgacc aacgcgggac gccgaatctg cgggtcagct
      901 cggcgcccac ccactcggcg tccgcggtgg gaagcatggc ggtgatcccg cgccgcatcc
      961 gctcgctgac cgcctcgacg gtcggccgcg ggctatgccc ggccatcgcg ccggtgtcac
     1021 cgagtgcgaa gtcgacgtag tcgtgcccgt cgacatcggt gatccggttg ccgctcgcac
     1081 cgtcgaggta gagcgggaag ccgccgctcc actttgccat ccaggtcatc ggcacccggc
     1141 gcacgaggtg ggtcgccgac cgattcatcg cagcagcgac ggggtgggta gcgatgtaca
     1201 tcgcccgctc gccctcgatg agggacgtca gtctgctccg atccactgtc ggcatggctc
     1261 ctacgtcagc tgattcgcag cacgtgcagg ccggaaccga cggccagcac gacggtcggg
     1321 tagggcccgg tcacggtgct ggcgatggtg ttgtcgggca acgcgagcgg gtctccgaca
     1381 tcccccgcag gcaacgcgaa tctgcgaacg cacgcagtct ggtccgcagt acaggcccct
     1441 gtgtcggggt tgccgaccac gaacagaccg ctggcagtca ccgcaattgg ttcggctgcg
     1501 gagaagttga tctgcgccgt cggtccgacg agcgtggtcg cgtcataggt gttgagctgc
     1561 gcgtcgaggc cctgcccggc atcgtgctgc acgatgacgt ggccgtcggc gacggcgatc
     1621 gcctggatcg ccgaaggtcc ggacagctgg agattgacct gctggttggt cggcgcggtg
     1681 atcgtggcgc cggcgttggt ttgttccacc aactggctgc tctgcttcgt cacgtagtac
     1741 acgcccgccg ggccggcggc gatcgagaac gcgccggtga aaccggtcac cgaacgcgcc
     1801 ggtgagctcg aggacgggtc gagctcgacg agaccgtccg gtgccccggt cgaattgtcg
     1861 ttggactgcg tcgacaggcc ccacaccgag gtgcccgcca cgacgagctg ggtcagcgtg
     1921 ccgggcaacg cggtgtgcca ggtgcgcacc acattgccgg tcgttctgct ataggcgatg
     1981 acggactgcc cgaccccgac gtacagattc gcggcatccg cggccagcgc ggtgaccgga
     2041 ccttgcacgt gttcggcgat ccctgctggc tgtactccgt cgacgaccca gatgacctgc
     2101 ggcgaggcga ccttgccggc gacgaagatc gcgccgtccg gcgcctgtgc gacggtaagg
     2161 cccgtctggg cggaggagaa cgcgccctcg atggccacgt cggcgaagat cttcagcggt
     2221 ccggtggggc cgggagtcgg caagggattc aagctggacg ccgcgggcag cggcgtcgct
     2281 tgggtcgagc ccgatcccgc tgacgccgat acgcccgacg acgcggctgt cgcc
//
LOCUS       DATCBJ010000570         4268 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_17688, whole genome shotgun sequence.
ACCESSION   DATCBJ010000570 DATCBJ010000000
VERSION     DATCBJ010000570.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 4268)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4268)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4268
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_17688"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..936)
                     /gene="guaA"
                     /locus_tag="VII50_11455"
     CDS             complement(<1..936)
                     /gene="guaA"
                     /locus_tag="VII50_11455"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HEY5051510.1"
                     /translation="MSEATGPTVLVVDFGAQYAQLIARRVREADVYSEIVPHTMSVEE
                     IVAQKPAAVILSGGPSSVYAEGAPSIDASLFDTGIPVFGICYGFQAMAQALGGVVERT
                     GLAEFGGTKVTVVPSSEGLLFRDLPTQQSVWMSHGDSVTAAPDGFTVTAASDGAPVAA
                     FEDLSRRLAGVQFHPEVRHTEHGQTVLRRFLREVAGIEPSWTTAGIVADQVAGIRSQV
                     GSGRVICGLSGGVDSAVAAALVQRAVGDQLTCVFVDHGLLRKGEAEQVERDFVASTGV
                     RLHVVEASSVFLSALAGISDPETKRKIIGREFIRAF"
     gene            1190..2335
                     /locus_tag="VII50_11460"
     CDS             1190..2335
                     /locus_tag="VII50_11460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5051511.1"
                     /translation="MIVLALSVPLGVVAHQTSVGLVQIALTAPFALVGFIVARRQPAN
                     CIGWIMLVIALLSNVGADAGEYSVLAFDHGHPGLPLARLAVALTQLWVALLVLLPIPI
                     VAFPDGRLRSVGWRRVFRTYLAVCAFFVVAVAVKDSAAFTDRHIAIDSSGELKTFTES
                     SQGAGAALGAALFLLYAIFGLSFVVGQLFAYRHATGERRQQLKWLISGGVVGIVGFSL
                     LLILSTSNVAALRLLAAVGYLGVIAVPLSIGVGILKYRLYDIDRLISRTLSYAVVTGL
                     LIGIYIGVVTLTTRALPLSSPIGVAASTLTVAALFSPLRRRAQRLIDHRFNRARYDAD
                     ATVAAFSSRLRDEVDLNRVQGDLLDVVHRSVQPSHAAIWIPRERAAS"
     gene            2386..3510
                     /locus_tag="VII50_11465"
     CDS             2386..3510
                     /locus_tag="VII50_11465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5051512.1"
                     /translation="MTAITFALLIGGFPLQKLAHQSLNAGNLLMLPFALVGAIVAIRQ
                     PRNPIGWIMLALAMLFLTSTDAGLYAVRSFRLDHKELPLARLAVACTQLWVGLLVLLP
                     LPILLYPDGRLPSRRWRSPFFGYIASCVVLVVGIGTKDVAAFTDKHIQVDSSGELVTL
                     GGNSKGTGAAIGGLLFLLYAGLALSFVCAQLLAYRRSTGDRREQLKWLMSGSTIGIIG
                     LAIALSPVMKAVGNVLSFGILAVPLSMGVGILKYRLYDIDRLISRTLSYAVVTGALVG
                     VYIGMVTLSTRALPLSSPIAVAASTLTVAALFNPVRRRAQRVIDRRFNRARYDADATI
                     AAFTSRLRDEIDLNQVQRDLVDVVNRSVQPSQVAVWINAD"
     gene            complement(3497..>4268)
                     /locus_tag="VII50_11470"
     CDS             complement(3497..>4268)
                     /locus_tag="VII50_11470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5051513.1"
                     /translation="HGAYGVGSALGPLVVTAAILITSWRPAYFLLIALELLLAYGWWK
                     TSSHWTPEMADDAVATEAAVPDDGPTRHVGLLVGLGVAAFFFYTGLEVTAGQWAASFF
                     RGPLGLSAGAAGPAVFAYWGSLTAARFGIAIPRRTPNPALLVRVGCIGGLGASALIWW
                     APSTAVVIVGFALLGAVLAPVFPALVTLTPVRIGHARAQHAIGWQIAGANVGAAGISA
                     FTGIILQHEGLRTLGPCLVVIAAALIGCNAALELFSRR"
BASE COUNT          746 a         1458 c         1288 g          776 t
ORIGIN      
        1 gaacgcgcga atgaactcgc gaccaataat cttgcgcttc gtttcgggat cactgatccc
       61 agcaagtgcg ctgagaaaca ccgaagacgc ctcgacgacg tgcagacgaa cacccgtcga
      121 cgcgacgaaa tcgcgttcaa cctgctcagc ttcgcccttg cgcaacaggc catggtctac
      181 gaagacgcac gtgagctgat cccccaccgc acgttgcacc aacgctgccg caacggccga
      241 gtcaacccca ccagacagcc cacaaatcac gcggccagaa ccgacctgcg aacggatgcc
      301 cgcaacctga tccgcgacga tgcccgccgt cgtccaactt ggttcgatgc ctgcgacctc
      361 tcgcaggaaa cggcgcagca ccgtctgccc gtgctcggtg tggcgcacct ccgggtggaa
      421 ctggacgccg gctaggcggc gcgacagatc ctcgaaggcc gctaccggcg cgccgtcaga
      481 cgcggccgtc accgtgaagc catccggcgc agcggtgacc gagtcgccgt gcgacatcca
      541 caccgactgc tgcgttggaa gatcccgaaa aagcagtccc tcagaagaag gcacgacggt
      601 gactttcgtg ccaccgaact cggccaaccc ggtgcgctcg accacgccgc caagggcctg
      661 tgccatggcc tgaaagccgt agcagatgcc gaagaccggg atcccggtgt cgaacagcga
      721 ggcgtcgatc gacggcgcac cctctgcgta gacggacgac ggcccgcccg acaagatcac
      781 cgcggccggc ttctgggcga cgatctcctc gacggacatg gtgtgcggca cgatctcgct
      841 gtacacatcg gcctcgcgca ctcgtcgcgc gatcagttgc gcgtactgcg ccccgaagtc
      901 gaccaccagc accgtggggc ctgtggcctc gctcacgcaa accgcctctg gcctcgggga
      961 tcgtgcgttg attctagagg gtcggtttcc gtagcgttgt cggaacgatt gccggttacg
     1021 gtcgtcctcg acccgagcga ttaccggagg gaggcaccgg atgaagtcga gcgcccgagc
     1081 aaccgatgag cccggtcggt cccgctgggc atcgccgggg gcctcctggg catcggcgct
     1141 gaggcgctgg gcaacgccga ggagcgcctc gctgctatcc gcgctgacag tgatcgtgct
     1201 cgcccttagc gtgccgctcg gggtcgtcgc gcatcagacc agtgtcggtc tcgttcagat
     1261 cgcgttgacg gcgccgtttg cgctggtggg cttcatcgtc gcgcgaaggc agcccgccaa
     1321 ctgcatcggc tggatcatgc tggtgatcgc cctgctctcc aacgtgggcg ccgacgccgg
     1381 cgaatactcg gtgctcgcgt tcgaccatgg tcaccctggg ttgccgttgg ctcgactcgc
     1441 ggttgcactc actcaactgt gggtcgctct cctcgtgctg ctgccaatac cgatcgtcgc
     1501 gtttcctgat ggcagactgc gatcggtcgg ctggcgtcgg gttttccgga catacctcgc
     1561 cgtctgtgcc ttttttgtcg tggccgttgc cgtgaaggac tccgccgcct tcacggatcg
     1621 acacatcgcg atcgattcgt ccggtgagct caagaccttc accgaatcga gccaaggcgc
     1681 tggcgcagcg ctcggtgccg ccctgttctt gttgtacgca atctttgggc tgtcgttcgt
     1741 ggtcgggcag ttgttcgcgt accgacacgc aacgggcgag cgccgccagc agctgaagtg
     1801 gctgatcagc ggcggtgtcg tcggtatcgt gggcttctcg ttgctgctca tcttgagcac
     1861 aagcaatgtg gccgcactca ggttgctcgc cgcagtcgga tacctcggtg tgatcgcggt
     1921 tccgctcagc atcggtgtcg gcatcttgaa gtaccggctc tacgacattg atcgactgat
     1981 cagcagaacg ctctcctacg ctgtagtgac gggactgctc attggcatct acatcggcgt
     2041 cgtcacgctg accactcgag cgctacccct gtcgtcaccg atcggcgtcg ccgcatcaac
     2101 gctgacggtt gccgcgctgt tcagcccact tcggcgacga gcccagcggc tgatcgacca
     2161 ccgcttcaat cgcgctcgct acgacgccga cgccactgtc gcggcttttt cctcgcgcct
     2221 acgtgacgag gtcgacttga accgcgttca gggcgacctc ctcgatgtcg tccaccgcag
     2281 cgtgcaaccg agccatgccg ctatctggat tccacgcgag cgggcggcat cgtgaacgcg
     2341 gcaacaggca ggcatccgtc gctcacgaag acgacggcgc tagcgatgac tgccatcacc
     2401 ttcgcgctac tcattggcgg ctttccgctg cagaagctag cccatcagag cctcaatgcg
     2461 ggcaacctgc tgatgcttcc gtttgccctc gtgggcgcca tcgtcgcgat ccgccaaccg
     2521 cgcaatccca tcggttggat catgctcgcc ctcgcgatgc tgtttctcac cagcaccgac
     2581 gccggcttgt acgccgtccg gtccttccgg ctcgaccaca aagagttgcc actcgcacgg
     2641 ctcgccgtcg cctgcaccca gctgtgggtc gggctgctcg tgcttttgcc gctgcccatc
     2701 ttgttgtacc ccgacggccg gttgccatcg aggcgctggc gatcgccgtt cttcgggtac
     2761 atcgcatcgt gtgtcgtgct ggtggtcggt atcggaacca aagacgtagc cgcgttcacc
     2821 gacaagcaca tccaggtcga ttcgtcgggc gaactcgtga ctctcggcgg caactcgaag
     2881 ggtaccggcg cggcgatcgg aggcctgttg ttcctcctct acgccgggct cgcattgtcg
     2941 ttcgtctgcg cacaacttct ggcatatcga cgctcgacag gtgatcgccg tgaacaactg
     3001 aagtggctga tgagcggcag cacgatcggc attatcggac tcgcgattgc actgtctccg
     3061 gtcatgaagg cggtcggcaa cgtcctctca tttggcatcc tcgccgtgcc gctgagcatg
     3121 ggcgtcggca tcctgaaata ccggctctac gacatcgacc ggttgatcag ccggaccctg
     3181 tcctacgccg tcgtcacggg agcactcgtg ggcgtctata tcggcatggt gaccctgtcg
     3241 actcgtgcac ttccactgtc gtccccgatc gccgtcgccg cttcgacgct cacggtggct
     3301 gcgctcttca acccggtgcg tcgccgcgcg cagcgggtga tcgatcgccg cttcaaccgc
     3361 gctcgctacg acgctgatgc gaccatcgcg gcgtttacgt cacgcctgcg cgacgagatc
     3421 gatctcaacc aggttcagcg ggacctagta gatgttgtca accgcagcgt gcaacccagc
     3481 caggtcgcgg tctggatcaa cgccgactga acaactccaa cgcggcattg cagccgatca
     3541 acgcggccgc aatgacgacg aggcacgggc ccagcgttcg caagccttcg tgttgcaaga
     3601 tgatgccggt gaatgccgat attcctgcgg ccccaacgtt ggcgccggcg atctgccagc
     3661 cgatcgcgtg ctgggctctc gcgtggccga tccgcactgg cgtcaacgtc accagcgcgg
     3721 gaaagaccgg cgcgagcacc gctcccagaa gtgcgaatcc cacgatgacc accgccgtcg
     3781 acggagccca ccagatcagc gccgaagcgc cgagcccccc gatgcaccca acccgcacga
     3841 gtagcgccgg attcggcgtc cggcgtggaa tggcgatgcc gaatcgggca gccgtcaacg
     3901 atccccagta cgcaaagacg gcaggtccag cagcgcccgc cgacaaaccc agcggcccgc
     3961 gaaagaagct cgccgcccac tgcccggcgg tgacctcgag gccggtgtag aagaaaaagg
     4021 ctgcgacacc aagaccaacc agtaggccga cgtgccttgt cgggccgtca tcgggcaccg
     4081 ccgcttcagt ggcaacagcg tcgtccgcca tctccggcgt ccaatgagaa cttgttttcc
     4141 accagccgta cgcgagcagc aactccaacg caattaacag gaagtaggcc ggtcgccacg
     4201 acgtgatcag gatcgcggcc gtgacgacaa gtgggcccag agccgatccc acgccgtagg
     4261 cgccgtgc
//
LOCUS       DATCBJ010000571         2280 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_125925, whole genome shotgun sequence.
ACCESSION   DATCBJ010000571 DATCBJ010000000
VERSION     DATCBJ010000571.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2280)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2280)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2280
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_125925"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..199)
                     /locus_tag="VII50_11475"
     CDS             complement(<1..199)
                     /locus_tag="VII50_11475"
                     /inference="COORDINATES: protein motif:HMM:NF026092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching N-terminal
                     domain-containing protein"
                     /protein_id="HEY5051514.1"
                     /translation="MTGSVEIRVGPPSLTVHADEQFLVCALDGTIAAGEQQGYFCVDT
                     RLVSDYELTLSKMPPTLLNSAV"
     gene            complement(531..1790)
                     /locus_tag="VII50_11480"
     CDS             complement(531..1790)
                     /locus_tag="VII50_11480"
                     /inference="COORDINATES: protein motif:HMM:NF014696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase family protein"
                     /protein_id="HEY5051515.1"
                     /translation="MTARSHAVDVRRRAGWLPDQDDLESWLAGHQARVEARGEQVVLH
                     PVLVEFQELMDTDPVVRMYVNQMIAQVPSTKSYRKRHLESVEEMVRLINEVLTMAPEF
                     GAAMVATPLAAILDWTMGTPAGFAAYRDPRVNAMLKKILTAWCEFLSSGDSLYVLNDS
                     PSGWKCAEAQRTVGMEQYEHDPHDEHWGFASWNDFFTRRFKDGERPVASPDDNKVIVS
                     ACESTPYGISTDVTRQGRFWIKSQPYSLQDMLANDDSVAQFVGGTVYQAFLSATNYHR
                     WHSPVAGTIVRAFVQEGTYYSEADSEGKDATEPTNSQSYLAHVAARAIILIEADDPVI
                     GLVAFVPVGMVEVSSCMIDSKMKPGYHVGKGEELGYFQFGGSTHCLVFRPGAIAEFTL
                     EAIPQPHDPKAPLVLVRSKLATANPTA"
     gene            complement(1821..>2280)
                     /locus_tag="VII50_11485"
     CDS             complement(1821..>2280)
                     /locus_tag="VII50_11485"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEY5051516.1"
                     /translation="PLGRHGPTKSGHVRAVPLIDQAMEPLDRLSRRELFTAPDDLVFA
                     GAVGGHLDGSALRKRFYSALDRAGLGAMRAKPEPITFHDLRHTFGTLAVQAFPLSDVK
                     AYMGHADIQTTMIYVHHVPKHDAAARLSAIVGAATGIDNVAMLASPRAED"
BASE COUNT          395 a          766 c          723 g          396 t
ORIGIN      
        1 cgaccgcgct attcaacagc gtcggcggca tcttcgacag agtcagctcg tagtctgaga
       61 ccagacgtgt gtcgacgcag aagtacccct gctgctcgcc agccgcgatc gtcccgtcca
      121 gcgcgcaaac caggaactgc tcgtcggcgt ggacggtcaa gctgggcgga ccgacccgga
      181 tctccaccga accggtcatc gcacccgctc cgccttgcca aaccaatcga cgaacggctt
      241 cccaagcgac ggcaaccggt cgaacccagc gtactgtccg actaactcaa ccccgtcgaa
      301 cgaccgcggc tccgagaacc ggtgcgctgg ctcgctcttc gaagatccct cgatcaacgc
      361 cagcatccat tcgccaagcc caagcgctcg gtgctgctgg cgatgtccac gtgttgacgg
      421 cgctgcagca ccttgtcggc gtgacgcgta cgccgaagct tccttcctac ccgttgaaac
      481 gtgcagcttc gactccttcc tcaccgcgtg agacagagat tcgccaggcc tcaggccgtg
      541 gggttggcgg tggcgagctt cgagcggacg agcaccagcg gcgccttggg gtcgtgcggc
      601 tgcgggatcg cctctagggt gaattcggcg atcgcgcccg ggcgaaacac gaggcaatga
      661 gtcgagccgc cgaactggaa gtagccgagt tcctcgccct tcccgacgtg gtaaccgggc
      721 ttcatcttcg agtcgatcat gcaggacgac acctccacca tgccgaccgg gacgaaggcc
      781 accaggccga tgacggggtc gtcggcctcg atgaggatga tcgcccgtgc cgcgacgtgc
      841 gcgaggtagg actgcgagtt cgtgggctcg gtcgcgtcct tgccctccga gtcggcctct
      901 gagtagtagg tgccctcctg gacgaacgcc cggacgatgg tgccggccac cgggctatgc
      961 cagcggtggt agttggtcgc actcagaaag gcctgataga ccgtgccgcc gacgaactga
     1021 gcaaccgagt cgtcattggc cagcatgtcc tgcagcgaat acggctggct cttgatccag
     1081 aaccgacctt gccgcgtcac gtccgtgctg atgccgtagg gcgtcgactc gcaggcgctg
     1141 acgatcacct tgttgtcatc gggcgacgcc accggacgct cgccgtcctt gaagcgtcga
     1201 gtgaagaagt cgttccatga ggcgaagccc caatgctcgt cgtgcgggtc gtgctcgtac
     1261 tgctccatgc cgaccgtgcg ctgggcctcg gcgcatttcc agcccgacgg cgaatcgttg
     1321 agcacataaa gcgagtcgcc gctgctgagg aactcgcacc aggcggtcag gatcttcttg
     1381 agcatcgcgt tgacccgggg gtcgcgatag gccgcaaacc cggctggtgt tcccatcgtc
     1441 cagtcgagga tcgctgcgag cggcgtagct accatcgcgg cgccgaactc gggcgccatc
     1501 gtcagcacct cgttgatcag gcgcaccatc tcctcgacgc tctccaggtg tcgcttgcgg
     1561 tacgacttgg tgctcggcac ctgggcgatc atctgattca cgtacatgcg cacgacagga
     1621 tcggtatcca tcaactcctg aaactcgacc agcacaggat ggagcacgac ctgctcgccc
     1681 ctagcctcca cccgcgcctg gtggccggcg agccaggact cgaggtcgtc ctggtccggc
     1741 agccagccgg cccggcgccg cacgtcaacc gcatgggagc gagccgtcac ggaagggacg
     1801 atacccgcgg tcgccccatc tcagtcttcg gcccgcgggc tggccagcat cgccacgttg
     1861 tcgatccccg tcgctgctcc cacgatcgcc gacagcctgg ccgcggcgtc atgcttcggt
     1921 acgtggtgca cgtagatcat cgtggtctgg atgtcggcgt gccccatgta ggccttgacg
     1981 tccgacagtg ggaaagcctg gaccgcaagc gtcccgaaag tgtggcgcag gtcatggaac
     2041 gtgatcggct cgggctttgc acgcatcgct cccaaaccgg cccggtcgag tgccgagtag
     2101 aagcgtttgc gcagcgccga gccgtcgagg tgaccgccga ccgcccccgc gaacacgagg
     2161 tcatcagggg cggtgaacag ctcgcgtcgg ctaaggcgat cgagcggttc catcgcttgg
     2221 tcgatcagcg gtaccgcacg cacgtgcccg gactttgtag gtccgtgccg gccgaggggg
//
LOCUS       DATCBJ010000572         2617 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_310556, whole genome shotgun sequence.
ACCESSION   DATCBJ010000572 DATCBJ010000000
VERSION     DATCBJ010000572.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2617)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2617)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2617
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_310556"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..740
                     /locus_tag="VII50_11490"
     CDS             <1..740
                     /locus_tag="VII50_11490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009084198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="magnesium chelatase domain-containing protein"
                     /protein_id="HEY5051517.1"
                     /translation="VLVGHVTKDGAVAGPRTLEHLVDVVLSFEGDRHSRLRLIRTVKN
                     RYGPADEVGCFDLTEEGIVGLADPSGLFLSRRGKPVSGTCVTVALEGRRPLVAEVQAL
                     VASSSLAMPRRTTSGLDAARVSMVLAVTERRAKILLSGSDIFTATVGGVKMSEPAGDL
                     AVALAVASAHKDIPLRPGLVAIGEVGLAGEVRRVEGVGRRLTEAARLGFTLALVPPDS
                     GPLPPGVRSIEVADLGQAIEYAIATTG"
     gene            774..1871
                     /gene="disA"
                     /locus_tag="VII50_11495"
     CDS             774..1871
                     /gene="disA"
                     /locus_tag="VII50_11495"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA integrity scanning diadenylate cyclase DisA"
                     /protein_id="HEY5051518.1"
                     /translation="MPTNDRFDRTVDEKLRATLATIAPGTDLRDALERILRGNTGGLI
                     VLGYDKTVESLCTGGFNLDIETSAPLMREICKMDGAIVLDNTGTRILKANVHLQPDPS
                     IPTSESGTRHRSAERTAKQTGYPVISVSQSMRIIALYVGGRRYVLDDSGAILSRANQA
                     LATLERYKLRLDEVSGTLSALEIEDLVTVRDAAAVAQRLEMVRRIASEIEGYVVELGT
                     DGRLLSLQLDELLAGVEPERELVVRDYLPSGSKKSRTPDDVLRDFDMLSASELLDLST
                     IARVMGYAAGADALDAPVSPRGYRLLAKVPRLPNLVVDRLVEHFGGLQKLLAAGIDDL
                     QAVEGVGENRARSVREGLSRLAESSILERYV"
     gene            complement(1891..2307)
                     /locus_tag="VII50_11500"
     CDS             complement(1891..2307)
                     /locus_tag="VII50_11500"
                     /inference="COORDINATES: protein motif:HMM:NF013731.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HEY5051519.1"
                     /translation="MIGYITFRLGDRNLACRLDEVREVVRLDGLDVLPGMTPPISGLL
                     ELRGNPLPVADLRVGSYPGARGDVLVMASGVDAMGAIVDQVIAVHDEDELVSSGERPS
                     GLPDYVIDVLRHNGISTGRGGPVLLVHLQALLSVAV"
     gene            complement(2304..>2617)
                     /locus_tag="VII50_11505"
     CDS             complement(2304..>2617)
                     /locus_tag="VII50_11505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5051520.1"
                     /translation="VNLGRDADAIRELRQAVYLQPDSASALLLLAGALDRQGEPAAAG
                     RAYRSAAATVGDLPPEEVSAFFGGRSAAELVALCLRLATQAERTAKATKSVRFRTRET
                     P"
BASE COUNT          464 a          876 c          853 g          424 t
ORIGIN      
        1 ccgtgctcgt cggtcacgtg acgaaggacg gtgccgttgc cgggccgcgc acgctggagc
       61 acctcgtcga cgtggtgctg agtttcgagg gggaccggca ctcccggctg cggctgatcc
      121 ggaccgtgaa gaaccggtac ggcccggccg acgaggtcgg ctgcttcgac ctgaccgagg
      181 aaggcattgt cgggctcgcc gatccgagcg gcctgttcct ctcgcgacgt ggcaagccgg
      241 tgtcgggaac ctgcgtaact gtggcactcg agggtcgccg tccacttgtg gcagaagtgc
      301 aagcgttggt tgcgagctcg agcttggcga tgccgcgacg cacgacgtcg ggtctcgacg
      361 ccgcgcgggt atccatggtg ctggcggtaa cagagcgacg ggcaaagatc ctgctgtcgg
      421 ggtcagacat cttcaccgcc accgtcggcg gcgtgaagat gtctgaaccc gccggagacc
      481 tggctgtcgc gctcgccgtc gcgagcgcgc acaaagacat accgttgcgt ccaggtctcg
      541 tcgccatcgg tgaggtcggc ctggcgggtg aggtacgccg cgtcgaagga gtcggtcgcc
      601 ggcttaccga agccgcacga ctcggcttca ctctcgcact ggtgccaccg gactcaggac
      661 cgctaccacc cggcgtccgt tcgatcgaag ttgccgacct cggacaagcg atcgagtacg
      721 cgatcgcgac caccggctag aatcgcggta acggcgtaca acaggggagc ccggtgccaa
      781 ccaacgatcg gttcgaccgg accgtcgacg agaaattgcg agccactctc gcgacgatcg
      841 cgcctggcac cgacctgcgc gatgcacttg aacgcatcct gcgcggcaac accggcggtc
      901 tgatcgtgct cggttacgac aagaccgtcg agtcgctgtg caccggcggt ttcaatctcg
      961 acatcgagac ttcggcgccg ctgatgcgcg agatctgcaa gatggacggt gccatcgtgc
     1021 tcgataacac cggcacccgg atcctcaaag cgaacgtgca cttgcagccg gacccgtcga
     1081 tcccgacgag cgagtcgggc acccggcacc gcagtgccga gcgcaccgca aagcaaacag
     1141 gttatccggt gatctcggtg agccagtcga tgcgcatcat cgcgctctac gttggcggca
     1201 ggcgctatgt cctcgacgat tccggtgcga tcctctcgcg ggcgaaccag gcactggcga
     1261 ctctcgagcg ctacaagctg cgcctcgacg aggtgagcgg aaccctgtct gcgctggaaa
     1321 tcgaggacct tgtcacggtg cgcgacgccg ccgcggtggc gcagcgcctc gagatggtgc
     1381 gccggatcgc gagcgagatc gagggctacg tcgtcgagct cggtaccgat ggccggctgc
     1441 tcagcctgca actcgacgag cttcttgccg gcgtcgaacc ggagcgcgaa ctcgtcgtcc
     1501 gtgactacct gccttcagga agcaagaagt cgcgcacgcc cgacgacgtg ctgcgcgact
     1561 tcgacatgct gtcggcatct gagctactcg acctcagcac gatcgctcgg gtgatgggct
     1621 acgccgccgg agcggacgca ctcgacgcac ccgtgagccc acgcggctat cggctgctcg
     1681 ccaaagtccc gcggctgcct aacctcgtcg tcgatcgcct tgtcgaacat ttcggtggct
     1741 tgcagaagct gctcgccgcg ggcatcgacg atctacaggc ggtcgaaggc gtcggcgaga
     1801 acagggcgcg cagcgtgcgt gagggtctgt cgcgactcgc cgaatcgtcg atcctcgagc
     1861 gctacgtctg agctgctgcg tgagcggggt tcacaccgca acggacagca gcgcctgcag
     1921 gtgtacgagc agcaccggcc cgccgcgtcc agtgctaata ccgttatggc ggagtacgtc
     1981 gatgacgtag tccggcagcc ctgacggtcg ctcgcctgac gagaccaact cgtcctcgtc
     2041 gtgaactgcg atgacctgat cgacgatcgc gcccatcgcg tcgacgcccg aggccatcac
     2101 caacacgtcg cccctcgcac ccggatagct gcctactcgc aagtctgcga ccgggagcgg
     2161 attgccacgc aactccagca gtccgctgat cggcggcgtc atgccgggca ggacatcgag
     2221 cccgtcgagc cggacgacct cgcgtacctc gtcgagccgg cacgcgaggt tccggtcgcc
     2281 gagacgaaac gtgatgtaac cgatcatggc gtctcccgcg tcctgaacct gaccgacttg
     2341 gtggcctttg cggtccgctc ggcctgcgtc gcgagccgca gacacagggc aaccagctct
     2401 gcggcactgc ggccaccaaa gaacgcggac acctcctccg gaggcaagtc accaaccgtt
     2461 gcagcagccg atcggtaggc ccggcctgcg gcggctggtt cgccctgccg atcaagcgcg
     2521 ccggcaagca gcagcagggc cgatgccgaa tcgggctgaa gatagacggc ctgacgcaac
     2581 tccctgatcg cgtcagcgtc gcgacccaga tttacca
//
LOCUS       DATCBJ010000573         2272 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_53331, whole genome shotgun sequence.
ACCESSION   DATCBJ010000573 DATCBJ010000000
VERSION     DATCBJ010000573.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2272)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2272)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2272
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_53331"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..288)
                     /locus_tag="VII50_11510"
     CDS             complement(<1..288)
                     /locus_tag="VII50_11510"
                     /inference="COORDINATES: protein motif:HMM:NF019928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HEY5051521.1"
                     /translation="MTPSRHGSAVIEYPNDLEIVLTRDFDAAIGLVFDVFTKPEHVRK
                     TFAPFGEEVTACSIDLRVGGKYHIVMVTADGIECSFRGTYLEVEPPTRTVET"
     gene            complement(278..625)
                     /locus_tag="VII50_11515"
     CDS             complement(278..625)
                     /locus_tag="VII50_11515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009073927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEY5051522.1"
                     /translation="MARSPTTSDVFNAIAEAQRRDVLDVLNTGEKAVGAIVKDLAMSQ
                     PQVSKHLRVLRAVGLVTCRAEGRRRLYRLERARLRPLHEWLANYEQAWNDRLDRVDDL
                     LKELQQKESVNDT"
     gene            complement(776..852)
                     /locus_tag="VII50_11520"
     tRNA            complement(776..852)
                     /locus_tag="VII50_11520"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(816..818),aa:Glu,seq:ctc)
     gene            complement(889..>2272)
                     /locus_tag="VII50_11525"
     CDS             complement(889..>2272)
                     /locus_tag="VII50_11525"
                     /inference="COORDINATES: protein motif:HMM:NF017555.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DALR anticodon-binding domain-containing
                     protein"
                     /protein_id="HEY5051523.1"
                     /translation="IDDTGVQVADVVVGIRELGIEQAPGEPFDQYCSRVYIAVSARYE
                     TDPDLLEQRRAVLHEVEEGGNETAVFVKDVARRIVNAHLATMRRFDISYDLLTWESDI
                     IGLGFWRQAFELLRESGAVVLVETGKLAGCWVMPADGADDEGDDDTKVLVKSDGIATY
                     TAKDIAYQLWKFGLLGRDFQYVPWEDDPTLMTTSSAPQAGVPSAHFGHAQRVINVIDT
                     RQAYPQRVVQRALRRLHHEREADSSIHLSYEVVALTPAAAAELGVVIEEGRDSVALSG
                     RRGIEVRADDLLDRAITRLSANARDAAGATLVAAGAVRYYLLKFSLNQIIAFDFDEAL
                     RVTGDTGVYLQYAHARAAGILRKVSDDGGLAAIPAQLHPSERELLHSIEAYSRALTET
                     ALALSPSLLTTYAFSLASAFSDFYEHTPAIVREEDAAIRRFRRALVAATRATLADALH
                     TLGMASPDQV"
BASE COUNT          402 a          736 c          746 g          388 t
ORIGIN      
        1 tgtctcgacg gttcgggtcg gtggctcgac ctccaggtag gtaccacgaa acgaacactc
       61 gattccgtcg gcggtgacca tgacgatgtg gtacttcccg ccgacacgga ggtcgatcga
      121 gcacgccgtc acctcctcac cgaagggggc gaaggtcttc ctcacgtgct ccggttttgt
      181 gaagacgtcg aagacgagtc caatcgcagc gtcgaagtct cgcgtgagga cgatctcgag
      241 atcgttcggg tattcgatga ccgccgaacc gtggcgacta ggtgtcattg acgctctcct
      301 tttgttgtag ctccttgagg aggtcatcca ctcgatccaa tcggtcgttc cacgcctgct
      361 cgtaattggc caaccactcg tgcaacggcc tgaggcgggc gcgctccagg cggtacagcc
      421 gccgacgccc ctccgcccgg catgtgacga gtcccaccgc acggagcacc cgaaggtgtt
      481 ttgaaacctg aggctgcgac atcgcgaggt ctttcacgat cgctccgacg gccttctcgc
      541 cggtgttcag gacgtccagg acgtcgcgcc gctgtgcctc ggcaatcgca ttgaacacgt
      601 cggacgtcgt gggagatcgg gccacggcca cattatatgc cgatagcgac atgtatcagg
      661 gtcgacgctt catccacgac gaagtccacg cccctggaac tccacgagac ggatcgaaca
      721 ggcgaagaaa cctcttgcca acgcgcgcgg cgatcgagcc acggctgcgc ctcggtggta
      781 ccccgtatcg gattcgaacc gatggtctcc gccttgagag ggcggtgtcc tgggccacta
      841 gacgaacggg gcccgaaggc ccggatcata ccagcgcccg gagacggctc agacctggtc
      901 gggtgaggcc atccccaggg tgtggagcgc gtcggcgagc gtggcccggg tcgccgcgac
      961 gagcgcacgc cggaaccggc gtatcgccgc atcctcttcg cggacgatcg ccggcgtgtg
     1021 ctcgtagaag tcgctgaacg ccgacgcgag actgaatgcg taggtggtga ggagcgacgg
     1081 cgacagtgcg agcgctgtct ctgtgagcgc ccgcgagtac gcctcgatgc tgtgcagaag
     1141 ctcgcgctct gacgggtgca gctgggcagg aatcgccgcc aacccgccgt cgtcgctgac
     1201 cttgcgaagg atgccggcgg cacgcgcgtg cgcgtattgc aggtagacgc cggtgtcacc
     1261 ggtgacacgc agcgcttcat cgaagtcgaa cgcgatgatc tggtttagcg agaacttgag
     1321 caggtagtag cgcaccgcgc cggccgcgac cagcgtggcg ccggcggcgt cgcgggcgtt
     1381 cgcgctcaga cgcgtgatgg cacggtcgag caggtcgtcg gcacgaacct cgatgccgcg
     1441 gcgcccgctc aacgccacgc tgtcgcgacc ctcctcgatc acgacaccga gctcggcagc
     1501 ggccgctgga gtcagcgcaa ccacctcata gctcagatga atcgagctgt ccgcctcgcg
     1561 ttcgtggtgc aaccggcgca gcgcgcgctg caccacgcgc tgcggatacg cctgacgtgt
     1621 gtcgatgaca ttgatcacgc gctgcgcgtg gccgaagtgg gcagagggga cgcccgcctg
     1681 cggggccgac gaggtggtca tgagggtcgg gtcgtcctcc cacgggacgt actgaaagtc
     1741 acggccgagc aagccgaact tccagagctg gtaagcgata tccttggccg tgtaggtggc
     1801 gataccgtcg gatttgacca gaaccttggt gtcgtcgtcg ccctcgtcat ccgcaccgtc
     1861 ggccggcatc acccagcatc ccgcgagctt gcccgtctcg acaaggacga ccgcacctga
     1921 ctcgcgaagc agttcgaatg cctggcgcca gaaaccgagc ccgatgatgt cggactccca
     1981 ggtgagcaga tcgtaggaga tgtcgaatcg acgcatggtg gcgagatgcg cgttgacgat
     2041 ccggcgcgcg acgtccttga cgaacacagc ggtctcgttg ccgccttcct ccacctcgtg
     2101 cagcacggcg cgacgctgct ccagcaggtc ggggtcggtc tcatagcgcg ccgacacagc
     2161 gatgtacacg cgcgagcagt actggtcgaa gggctcgccg ggtgcctgct cgatgccgag
     2221 ctcgcgtatc cccaccacga cgtcggccac ctggacgccg gtgtcgtcga tg
//
LOCUS       DATCBJ010000574         2776 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_448759, whole genome shotgun sequence.
ACCESSION   DATCBJ010000574 DATCBJ010000000
VERSION     DATCBJ010000574.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2776)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2776)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2776
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_448759"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..890
                     /locus_tag="VII50_11530"
     CDS             <1..890
                     /locus_tag="VII50_11530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HEY5051524.1"
                     /translation="ARMRIDLHVHSNASDGTDSPGDLVRKAWALALDGAPLDGVALTD
                     HDTITGYRDAADAVQGLVGGQGRPFIVVPGAEISCILGDVSLHLLGYLFDPANAQLAA
                     ELGLLRTDRIRRAKAMVDKLVELGAPVTWEQVSALAGDGAVGRPHIAQALVDAGVVPD
                     IPAAFVPEWIANDGRAYVEKYSLAPARAIALVQAAGGVTVFAHPAASARGAVVDEAAI
                     ETFASAGLDGLEVDHPDHDGPTRARLRAVASDLGLLVTGSSDYHGSVKSVEMGANTTD
                     LEVFETLVQRSPGGQVFSR"
     gene            887..1501
                     /locus_tag="VII50_11535"
     CDS             887..1501
                     /locus_tag="VII50_11535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009072963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarC family protein"
                     /protein_id="HEY5051525.1"
                     /translation="MSAWFDVRFFGEVFVTLLVIMDPPGSVPVFLSLTGSYARKARNR
                     SADLAVLTALTVIVVFALFGQQVLQYLHVTLAALQVAGGLLLLLVALELLMGWGNEHA
                     RKDDVNVSMVPLGTPLLAGPGAIAATIVFAKSVHHEQEVLALAAGIVAVHIVLWLTLR
                     FSTVILRVLGRSGVTLLTRIAGLLLSSIAVQLAADGIAKFVAAQ"
     gene            1470..2324
                     /locus_tag="VII50_11540"
     CDS             1470..2324
                     /locus_tag="VII50_11540"
                     /inference="COORDINATES: protein motif:HMM:NF037959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
                     /protein_id="HEY5051526.1"
                     /translation="MASRSSLPRSKPPSGGRRANAESFPVDTATATLVRDDSRRNAWT
                     LLLDGVETSHVDLDDPMSLEFEYVRWLGDVIECVAPAGRRVDTVHLGGGGLTLARYVM
                     ATRPQSTQIVFEIDGALVDLVRLRLPWQRSRRLRIRVGDARAGLAAVQDASCDLVVRD
                     AFRDAVVPASLRTVECATRVKDCLRKGGIYVMNVADRAPFATTAVELATVSEAFSDVA
                     VISEPGVLRGRRHGNLIIVAANEPLPVTAMARRVADGGVQGRIRDRGQAMEMARGHRP
                     LRDADLSM"
     gene            complement(2314..>2776)
                     /locus_tag="VII50_11545"
     CDS             complement(2314..>2776)
                     /locus_tag="VII50_11545"
                     /inference="COORDINATES: protein motif:HMM:NF012245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HEY5051527.1"
                     /translation="AQQTNLLALNATIEAARAGEAGKGFAVVADEVKDLAQETARATD
                     DISRRIESVRNDSRDAIAAIEAIRVVISQISDYQVTIASAVEEQSATTSEMGRGFSEV
                     ATGSAEIARSIAGVASASSTTLTSVASARVTTDELRKVADELKSLVSRFTY"
BASE COUNT          419 a          875 c          955 g          527 t
ORIGIN      
        1 gagcccgcat gcggatcgac ctccacgtgc acagcaacgc ctccgacggc acggattccc
       61 cgggagatct cgtccgcaag gcctgggcgc tcgccctcga tggcgcccct cttgacggtg
      121 tcgcgctgac cgaccacgac acgatcactg gctatcgtga cgctgcagat gcggtgcagg
      181 ggctggtcgg cgggcagggg cgtccgttca tcgtcgtccc gggagccgag atctcctgca
      241 tcctcggcga cgtcagcctg catctgcttg ggtatctgtt cgatcccgcg aatgcgcagc
      301 tggcggccga actcggcctt ctgcgcaccg atcgaatcag gcgggcaaag gcgatggtcg
      361 acaagctggt cgagctaggc gcgccagtca cgtgggagca ggtgagtgcg cttgcagggg
      421 atggcgcggt gggccgtcct catatagcgc aggcactggt cgacgccgga gtggtgcccg
      481 acataccggc ggcattcgtg cccgagtgga ttgccaatga cgggcgggcc tatgtcgaga
      541 agtactcgtt ggctccggca agagcgattg cgttggtgca ggcagccgga ggagtgaccg
      601 tattcgcgca tcctgcggcg tccgctcgtg gtgctgtcgt cgacgaggct gcgatcgaga
      661 cgttcgcgtc ggcgggtctc gacgggctgg aggtcgacca ccccgaccat gacggaccga
      721 cccgagcgcg gttgcgcgcg gttgccagcg acctcgggtt gctggtcacc ggatcgagtg
      781 actaccacgg ttcggtgaag tcggtcgaga tgggcgcgaa cacgaccgac ctggaggtct
      841 tcgagaccct cgtacaacgc tcgcccggcg gccaggtgtt tagccgatga gcgcgtggtt
      901 cgacgtccgg ttctttggag aagtgttcgt cacgctgctc gtcatcatgg atccgcctgg
      961 ttcggtgccg gtgttcttgt cgttgacagg ctcctacgcc cgcaaggcgc gcaatcgcag
     1021 cgccgacctg gctgtgctca ccgcccttac cgtcatcgtg gtgttcgcgc tgttcggtca
     1081 gcaggtgctg cagtacctgc acgtcacact cgccgcgttg caagtcgccg gcggcctgct
     1141 acttctgctc gtggccctcg agctcttaat gggctgggga aacgagcacg cgcgcaagga
     1201 cgacgtgaat gtatcgatgg tgccgctcgg cacgccgctg ctggccgggc ctggtgcgat
     1261 cgcggcaacg atcgtgttcg ccaagtcggt gcaccacgag caagaagtac tcgccctcgc
     1321 agccggcatc gtcgcggtcc acatcgtgct gtggttgacg ttacggttct ctacagtgat
     1381 cttgcgggta ctcggtcgca gcggagtgac gcttctcacg cgcatcgccg ggctgttgct
     1441 gtcctcgatc gcggtgcagt tggccgccga tggcatcgcg aagttcgttg ccgcgcagta
     1501 agccgcccag cggtggacgc cgtgcgaacg ccgagagctt cccggtcgat acggcgaccg
     1561 cgaccttggt ccgcgacgat tcacgacgca atgcgtggac gttgttgctg gacggcgtcg
     1621 agacctcgca cgtcgatctc gacgatccga tgtcactgga gttcgagtac gtgcgctggc
     1681 tgggtgacgt cattgagtgc gtagcgcccg ccggacgtcg cgtcgacacc gtgcacctcg
     1741 gcggtggtgg gttaacgctc gcacggtacg tgatggcgac gcggccgcaa tcgacgcaga
     1801 tcgtgttcga gatcgacggc gcactcgtcg atctcgtccg gctacgactg ccgtggcagc
     1861 gcagccggcg gctgcgcatc agggttggtg acgcgcgcgc cggactcgct gcggtgcagg
     1921 acgcgtcgtg cgatctcgtc gtccgggatg cattccggga cgccgtcgtg ccggcgagct
     1981 tgcgtacggt cgagtgcgcg acacgcgtga aggattgcct ccgtaagggg ggtatctacg
     2041 tcatgaacgt tgccgatcga gcaccgttcg cgacgactgc agtcgaactg gcgacggtgt
     2101 cggaggcctt cagtgacgtg gcggtgatct cagaaccggg cgtactgcgc ggccgtcggc
     2161 atggcaacct catcatcgtg gcggcaaacg agccgctgcc cgtcaccgcg atggcgcgcc
     2221 gcgtcgccga cggcggggtt cagggcagga tccgagaccg cggccaggcg atggagatgg
     2281 cgcgtggaca ccgtccactg cgtgatgccg acctcagtat gtgaagcgcg agacgagcga
     2341 cttcagttcg tcggcgacct tgcgcagttc gtctgtcgtg acccgcgctg aggcaacgct
     2401 ggtcaatgtc gtcgacgagg cgctcgcgac gccggcgatc gacctggcaa tctctgcgct
     2461 gccggtcgcc acctcggaaa atccgcgacc catctcactc gtcgtcgccg actgctcctc
     2521 gactgccgag gcgatcgtca cctggtagtc gctgatctga ctgatgacga cccggatcgc
     2581 ttcgatggct gcaatcgcgt cgcgcgaatc gttgcgaacc gattcgatgc ggcgggagat
     2641 gtcgtcggtg gccctcgcgg tttcctgcgc gaggtccttg acctcgtccg cgacgaccgc
     2701 gaatcccttg ccggcttcgc ccgcacgtgc ggcttcgatc gtcgcgttca gggccagcaa
     2761 gttcgtctgc tgcgca
//
LOCUS       DATCBJ010000575         2172 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_30747, whole genome shotgun sequence.
ACCESSION   DATCBJ010000575 DATCBJ010000000
VERSION     DATCBJ010000575.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2172)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2172)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2172
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_30747"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..375
                     /locus_tag="VII50_11550"
     CDS             <1..375
                     /locus_tag="VII50_11550"
                     /inference="COORDINATES: protein motif:HMM:NF013715.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HEY5051528.1"
                     /translation="GMLAIPVLAGAGSAAMAGLIGHGTGFSNSLREAPVFYGLCAVGT
                     IGGMALSLLGVNPIKLLILVAVINGVTAGPFLIVTMLVSSDRKIMGEHVNGRLARWLG
                     WTTTVLMLIAAVALFATGGVSV"
     gene            complement(1049..1303)
                     /locus_tag="VII50_11555"
     CDS             complement(1049..1303)
                     /locus_tag="VII50_11555"
                     /inference="COORDINATES: protein motif:HMM:NF017088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042314 - bacteriochlorophyll binding
                     [Evidence IEA];
                     GO_process: GO:0019684 - photosynthesis, light reaction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRC-barrel domain-containing protein"
                     /protein_id="HEY5051529.1"
                     /translation="MIRASDLLGCVVHTESGKKVGRVHDLRAKTTESECLLIGLMVGR
                     GGMLARLAGGGAGAGPTRGDDVIPWHAITHLKEGQITIRD"
     gene            complement(1300..1671)
                     /locus_tag="VII50_11560"
     CDS             complement(1300..1671)
                     /locus_tag="VII50_11560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5051530.1"
                     /translation="MSDPPDLMDLAYRILDDQLVDVDGRRCGRADDIEFEGELGDPPH
                     LYAIISGSGSWHRRFPRRLRGIGARVFGSGVSGADVIRVPWNQIDEIGAVLKLKAKAR
                     DLGLAQGDYRLGERIAHLPKS"
     gene            complement(1671..>2172)
                     /locus_tag="VII50_11565"
     CDS             complement(1671..>2172)
                     /locus_tag="VII50_11565"
                     /inference="COORDINATES: protein motif:HMM:NF013715.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HEY5051531.1"
                     /translation="VFAPLHVSPGYLGTTALGAEQAYGAIGLLLALLGMLFAVGGAAV
                     ECAFSGAYSLAQFAGWEWGKYRRKRDAPRFTLTWLIFFALAFVIVETGVDPVQLTEYA
                     VIFSAVALPLTYLPVLLIARDPTYMGEHVNGIVGNILGWTFFVILLGVSLAAIPLMVI
                     THGGAS"
BASE COUNT          374 a          693 c          743 g          362 t
ORIGIN      
        1 ggcatgctcg ccatcccggt gctcgccgga gcaggctccg cggccatggc cggactgatc
       61 ggccacggaa cgggcttctc caactcgttg cgcgaagccc cggtgttcta cggactgtgc
      121 gcggtcggga cgatcggcgg catggcgctc agcctgctcg gcgtgaaccc gatcaagctg
      181 ctgatcctcg tcgcggtcat caacggcgtg accgccgggc cgtttctgat cgtcacgatg
      241 ctcgtgtcct cagaccgcaa gatcatgggc gagcacgtca acggccgact cgcccgctgg
      301 ctcggctgga ccacgacggt cctcatgctg atcgccgcgg ttgcgctatt tgcgaccgga
      361 ggcgtgtccg tgtagaaagc gaaagcgtcg aaaggacggg gccgtcgtgg cgtccgctcg
      421 tgctatggcg tcagccgtca cgcgacggca atcgacaggc cgaccgtgag ggtcgcgacg
      481 acgagcaaga tcaaatacgt gacgaggtag ccgcgctagt acccgatcag gccggcggcg
      541 acgatagtga tcgcgaccga cgacaagacg caggccagcg aggcgccgaa gatgaagagg
      601 cagcgctgga gaacggagcc gattagcgtc gccacggcat gaccatggcc tttcgccggt
      661 tgcgctttcc gtgaggcagg tactgaccgg cacccaatcc ggttgcgcag cgacggcggt
      721 gccgatcgcc gcggcgagaa tcttagacgg agtcgcatgt gtggcgagca ggccctccac
      781 cacacccagc gtcgaagtca ttccgtcaaa tgaccccaag atcgccttcc ttacggtcgg
      841 gctcctgatc gtgttcggct cagcgcgccg cgtaaggttc ccgcggcgcg atcgtcacgt
      901 catcactgta gggcgaagcg tcatcgccgc acaagcgccg gcgttggcta tgtattgcgg
      961 tgcgttgcgg gcggtcgctg cgccgtcggc acgatcgggg catggcatca gcctgcgcgg
     1021 cgtgaaccga tcaagctcgg caccgggact agtcgcggat ggtgatctgt ccctctttca
     1081 ggtgggtgat cgcgtgccag ggaatgacgt cgtcgccgcg cgtgggcccc gcgcctgcgc
     1141 cgccgccagc caagcgcgcg agcatcccgc cgcgtccgac catgagccct atgagtaggc
     1201 actcactttc ggtcgtcttc gcgcgcaggt cgtgaacgcg gccgaccttc ttcccggatt
     1261 ctgtgtggac gacgcagccg aggaggtcgc tcgctcggat catgacttcg gcaggtgggc
     1321 gatgcgctcg cccagccggt agtcgccttg ggcgaggccg aggtcgcgcg ccttggcttt
     1381 gagtttcagg acggctccga tctcgtcgat ctggttccac ggcacgcgga tcacgtcagc
     1441 gccgctgacg ccgctgccga atacccgtgc gccgatgccg cgcaggcgac gggggaatcg
     1501 ccgatgccag ctgcccgagc cgctgatgat cgcgtagagg tggggtgggt caccgagctc
     1561 gccctcgaat tcgatgtcgt ccgcgcgtcc gcaccgccgc ccgtcgacgt caacgagctg
     1621 gtcgtcgagg atgcggtagg cgaggtccat gagatcgggg ggatcgctca tcagcttgct
     1681 cctccgtggg tgatgaccat cagcgggatt gcggccagcg acacgccgag gaggatcacg
     1741 aagaacgtcc agccaaggat gttgccgacg atgccgttga cgtgctcgcc catgtaggtc
     1801 gggtcgcggg cgatcagcag aaccggcagg taggtcagcg gcagcgcaac cgccgagaag
     1861 atcaccgcgt actcggtcag ctgcacggga tcaacaccgg tttcgacgat gacgaacgcg
     1921 agggcgaaaa agatcagcca ggtaagcgtg aagcgcggcg cgtcgcgttt gcggcggtat
     1981 ttgccccatt cccagccagc gaactgcgcg agcgagtatg cgccggagaa cgcacactcc
     2041 acggccgcgc cgccgaccgc gaagagcatt cccaatagtg ccagcagtag tcctatggct
     2101 ccgtatgcct gctcggctcc cagcgcggtg gtgccgaggt agcctgggct gacgtgaaga
     2161 ggcgcgaaga cc
//
LOCUS       DATCBJ010000576         4424 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_97987, whole genome shotgun sequence.
ACCESSION   DATCBJ010000576 DATCBJ010000000
VERSION     DATCBJ010000576.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 4424)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4424)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4424
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_97987"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..465
                     /locus_tag="VII50_11570"
     CDS             <1..465
                     /locus_tag="VII50_11570"
                     /inference="COORDINATES: protein motif:HMM:NF014453.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HEY5051532.1"
                     /translation="RVGLHGRQFRVWKLRSMNCGSDLRVTQLMAEHGGYVPFYKMRED
                     PRITPVGRFLRRSSIDELPQLINVLKGEMSLIGPRPHVPAEVDQYAPEHHRRLLVRPG
                     ITGLWQVSGRSDVPRHEAVLLDLQYVDTWSLRRDLRILLKTAKVVATARGAY"
     gene            complement(462..890)
                     /locus_tag="VII50_11575"
     CDS             complement(462..890)
                     /locus_tag="VII50_11575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5051533.1"
                     /translation="MRRSPDPRELASVLHRLEGLQIVAQPAAAGWLPHAPDAERLPPT
                     SGDQVPTAGRRWRATLRGNVSARPHVSAFDPGRRGVVVLVLVVVSVVSVGRGNGQHDP
                     SASVLKALSTIAGRVSRTDQDGDIAISVTCGGLKVVTRRT"
     gene            complement(989..1852)
                     /locus_tag="VII50_11580"
     CDS             complement(989..1852)
                     /locus_tag="VII50_11580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009081050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HEY5051534.1"
                     /translation="MSRARKGTPAVAVVTDSTAYLPSGSAEFLDVQVVPLQVIVGGVV
                     RAEGEEIGPSEVAAVLHRGHSVSTSRPSPSLFAAAYHRAFAAGATSIVSVQLSAAMSG
                     TVDAARLAAADVGGDIRVIDSRSVGMGMGFAVLAAADAAVAGADVAAVVDAVTQTLGR
                     TQAIFYVDTLEYLRRGGRIGAARALVGSALAVKPLLCLRDGRIEPLERVRTASRAIAR
                     LEELAVSMAADEPVSIAVHHLDASPRAAQVVEALEARLPKAGDIITTEVGAVVGAHTG
                     PGMLAIVVCTR"
     gene            complement(1862..4324)
                     /gene="leuS"
                     /locus_tag="VII50_11585"
     CDS             complement(1862..4324)
                     /gene="leuS"
                     /locus_tag="VII50_11585"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HEY5051535.1"
                     /translation="MSETFDFAAIQDRWLPVWDELGPFRTGRPAADGSPRERRYMLDM
                     FPYPSGDLHMGHAEAYAFGDVVARYWQQRGYDVLHPIGWDSFGLPAENAAIARNQHPA
                     DWTYKNIETQAASFRRYAVSFDWSRRLHTSDPEYYRWTQWLFLRFFERGLAYRKASAV
                     NWCPHDQTVLANEQVIAGHCERCGTLVTKKTLTQWYFKITEYADRLLDDMRELEGSWP
                     ERVLTMQRNWIGRSVGAHVDFAIEGRAEPVTVFTTRPDTLYGATFFVVAADSALAAEL
                     CAPQQRAAFDAYVEQVRRLSDIDRMSTERVKTGVFLGRYAINPVNDERIPVWAADYVL
                     ADYGTGAIMAVPAHDQRDLDFALTFGLAVRTVVATDEANPSETGVATPGDGVHVNSGS
                     LDGLDKSASIDRIIVELEAKGAGKGAINYRLRDWLLSRQRFWGAPIPIVHCDVDGEVP
                     VPDAELPVRLPDLRGADLQPKGVSPLAAATDWVNTTCPKCGGPAKRDTDTMDTFVDSS
                     WYFLRYCSPHRDDVVFDPADVKMWMPVDQYVGGVEHAILHLLYSRFFVKVLYDMGLVE
                     FDEPFKALLNQGQVINQGKAMSKSLGNGVDLGEQLAAFGVDAVRMTMLFAGPPEEDID
                     WADMNVPALGKFLARAWRLAGEVSSEIGIDVTAGDLALRRVTHHTVHDVTHLLDTFRF
                     NVAIARIMELVNAIRKTVDVGPGPGDPAVREAVEAVAVMLSLFAPYTAEDAWARLGHQ
                     PTVFHAGWPVVDPELLVEESVVCVVQINGKVRDRLDVAPDIDEATLRELALAAPAVVK
                     ALDGRAVRTVIVRAPKLVNVVV"
BASE COUNT          768 a         1521 c         1438 g          697 t
ORIGIN      
        1 cgcgtcggtc tgcacggtcg gcaattccgg gtatggaaac tccggtcgat gaactgcggc
       61 tcggatctgc gggttaccca actcatggcc gagcacgggg ggtacgttcc gttctacaag
      121 atgcgcgagg acccgcggat cacgccggtt ggccggtttc tgcggcggtc gtctatcgac
      181 gagttgcctc agctcattaa cgtgctcaag ggtgagatga gcttgatcgg cccgcggccg
      241 cacgtgccgg ctgaggtcga ccaatacgcg ccagaacatc atcgccggct cctcgtgagg
      301 cccggtatca ctggcctgtg gcaggtcagc ggccgatccg atgtgcctcg ccacgaagcc
      361 gttctgctcg acctgcagta cgtcgacacc tggtcgcttc gtcgcgatct gcgcatcttg
      421 ctgaagaccg caaaggtcgt cgcgacggcg cggggtgcgt actaggtacg ccgcgtcacg
      481 accttcagac caccgcaggt gacggagatc gcgatgtcac catcctggtc ggtgcgactg
      541 acccgaccgg cgatcgttga cagcgccttc agcacactgg ccgacgggtc atgctggccg
      601 ttgccgcggc cgacagaaac gacagagacg acgacgagga cgaggacgac cacccctcga
      661 cgtcccggat cgaatgcgct gacgtgcggc cgcgccgaaa cattcccgcg caacgtggcc
      721 cgccagcgcc gtccggccgt cggcacctga tcccccgacg taggcggcaa ccgctccgcg
      781 tcgggcgcgt gcggcagcca tcccgcggca gcgggttgcg ccacgatctg caagccctca
      841 agccggtgca gcacgctggc cagctcgcgc ggatccggac tccggcgcat catcggcacg
      901 actcgagcta agcggtcggc cgccctacgg ataagaccga accacggctg tggaccgtgc
      961 cgacgactac aggcgactgt ggaaagcctc agcgcgtgca gacgacgatc gcgagcatgc
     1021 ctgggccggt gtgcgccccg acgactgcgc cgacctcggt cgtgatgatg tcgccggctt
     1081 tcggcaggcg ggcctccagc gcctcgacga cctgcgcggc acgcggcgac gcatcgaggt
     1141 gatgcaccgc gatgctcacc ggctcgtccg cggccatcga caccgcgagt tcctcgagtc
     1201 gggcaatcgc ccgcgaggcc gttctgaccc gctccagcgg ttcgatccgt ccgtcacgca
     1261 gacacagcaa tggcttcacc gcaagcgccg atccgaccaa ggcgcgggcc gcaccgatcc
     1321 ggccgccgcg gcggaggtat tcgagcgtgt cgacgtagaa gatcgcctgc gtgcgcccga
     1381 gggtctgggt caccgcatcg accacggccg cgacgtcggc gcccgcgacg gcggcgtcag
     1441 ccgcggccag caccgcgaac cccatgccca tgccgaccga ccgcgaatcg atgaccctga
     1501 tgtccccacc gacgtcagcc gcagccagcc gcgcggcgtc gacagtgccc gacatcgcgg
     1561 ccgacagctg cacggacacg atcgatgtcg cgcctgccgc gaatgcccgg tggtaggcgg
     1621 cggcgaacaa gctcggcgat gggcgggacg tgctcaccga gtgcccgcga tgcaggaccg
     1681 cggcaacctc gctcggtccg atctcctcac cctcggctcg cacgacgcca ccgacgatca
     1741 cctggagcgg cacgacctgg acgtcgagaa actccgcaga tcccgaaggt aggtaagccg
     1801 tcgagtctgt gacgaccgcc accgctggcg tgccctttcg cgcgcgtgac ataggcggac
     1861 cctagaccac gacgttgacg agcttcgggg cccggacgat gacggtgcga acggcgcgcc
     1921 cgtcgagggc tttcacgacc gcgggcgctg cgagcgccaa ctcgcgcagc gtggcctcgt
     1981 cgatgtctgg cgcgacatca agccggtccc gaaccttgcc gttgatctgg acgacgcaca
     2041 ccaccgactc ctcgaccagc aactccggat cgaccaccgg ccagccggcg tgaaacacgg
     2101 tgggctgatg cccgagccga gcccacgcgt cctcagcggt gtagggcgcg aacagcgaca
     2161 gcatcacggc caccgcttcg accgcctcac gcacagccgg atcccccggt ccagggccca
     2221 cgtcgaccgt cttgcggatc gcgttgacta actccatgat gcgcgcgatc gcgacgttga
     2281 accggaacgt gtcgagcagg tgcgtgacgt cgtgcacggt gtgatgggtg actcgtcgca
     2341 aggcgaggtc accggccgtc acgtcgatac cgatttcgct gctgacctcg cctgccaacc
     2401 gccatgccct ggccaaaaac ttcccgagcg ccggcacgtt catgtcggcc cagtcgatgt
     2461 cttcctcggg cggaccagcg aacagcatcg tcatccggac cgcgtcgacg ccgaacgccg
     2521 ccagttgttc acccaggtcg acgccattgc ccagtgactt gctcatggcc ttgccctggt
     2581 tgatgacctg accctggttc agcagcgcct tgaacggctc gtcgaactcg acgagcccca
     2641 tgtcgtacag cactttgacg aagaacctgc tgtacagcag gtgcaggatc gcgtgctcga
     2701 ccccgccgac gtactggtcg accggcatcc acatcttgac gtcggccgga tcgaacacga
     2761 cgtcgtcgcg atgtggcgag caataccgca ggaagtacca cgacgagtcg acgaacgtgt
     2821 ccatcgtgtc ggtgtcgcgc ttcgccgggc cgccgcactt cgggcaggtg gtgttcaccc
     2881 agtcggtcgc ggccgccagt ggtgacacgc ctttgggctg caggtcggca ccgcgcagat
     2941 cgggcagtcg caccggcaat tcggcgtccg ggaccggaac ctcgccgtcg acatcgcaat
     3001 gcacgatcgg gattggcgca ccccagaatc gctgccgtga cagcaaccag tcgcgcaacc
     3061 ggtagttgat cgcgcccttg ccggcaccct tggcttcgag ctcgacgatg atgcgatcga
     3121 tgctcgcact cttgtcgaga ccatcgagcg aacctgaatt cacatggacg ccgtcacctg
     3181 gcgtcgcaac gccggtctcc gatgggttgg cctcatcggt cgcaaccacg gtgcgtaccg
     3241 ccagcccgaa cgtgagcgcg aagtcgaggt cacgctggtc atgcgccggc accgccatga
     3301 tggcgccggt gccatagtcg gcaaggacgt agtccgccgc ccacaccggg atgcgttcgt
     3361 cgttgaccgg gttgatcgcg taccggccga ggaaaacacc cgtcttcacc cgctcggtcg
     3421 acatccggtc aatgtccgag agccgccgca cttgctcgac gtaggcatcg aacgcggctc
     3481 gctgttgcgg cgcgcacagc tccgcagcca gcgccgagtc ggcggcgacg acgaagaagg
     3541 tcgcgccgta caaggtgtcg ggccgggtgg tgaataccgt gaccggttcg gcgcggcctt
     3601 cgatcgcgaa gtcgacgtgt gcaccaaccg atcgaccgat ccagttgcgc tgcatggtca
     3661 gcacgcgttc gggccacgag ccctcgagtt cgcgcatgtc atcgagcagc cggtcggcat
     3721 attcggtgat cttgaagtac cactgggtca gcgtcttctt ggtgaccagc gtcccgcagc
     3781 gttcacagtg gccggcgatg acctgctcgt tggccagcac ggtctggtcg tgcgggcacc
     3841 agttgaccgc cgaggccttg cgatacgcga gcccgcgctc gaagaagcgc aggaacaacc
     3901 actgggtcca gcggtagtac tccgggtcgc tggtgtgcag ccggcgcgac cagtcgaacg
     3961 aaacggcata acgccgaaac gacgccgcct gggtctcgat gttcttgtag gtccagtcgg
     4021 ccgggtgctg gttgcgggcg atcgcggcat tctcggccgg cagaccgaat gagtcccagc
     4081 cgatcggatg caggacgtcg tagccccgct gctgccagta gcgcgcgacg acatcaccga
     4141 aggcgtacgc ctcggcgtga cccatgtgca ggtcgcccga cggataggga aacatgtcga
     4201 gcatgtagcg gcgctcgcgt ggcgagccgt cagcggccgg gcggccggtg cgaaacgggc
     4261 cgagctcgtc ccagaccggc agccatcgat cctggatggc ggcgaagtcg aacgtctcgc
     4321 tcatcggttc ctcacctggg tgttgcggca gtcgatccag acacaaaaag acccctcgcg
     4381 cacgaggggt tgccgcgccg acgtcgggtc agcgcggcgt aatg
//
LOCUS       DATCBJ010000577         2290 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_207227, whole genome shotgun sequence.
ACCESSION   DATCBJ010000577 DATCBJ010000000
VERSION     DATCBJ010000577.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2290)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2290)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2290
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_207227"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..632)
                     /gene="rarD"
                     /locus_tag="VII50_11590"
     CDS             complement(<1..632)
                     /gene="rarD"
                     /locus_tag="VII50_11590"
                     /inference="COORDINATES: protein motif:HMM:TIGR00688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter RarD"
                     /protein_id="HEY5051536.1"
                     /translation="MRDTRRGFGYGLAAYTIWGLFPLYWPLLDPTPAIQILAHRMLWS
                     LVFIGTLLAARRRWAFVGELRHDLKRVGLLVLAGVLISVNWGVYIWAVNAGHVVESSL
                     GYFITPLVNIGLGVLVLKERLQRTQWVAVIIGVVAVLVIAVGYGQVPWIGLTLAASFG
                     GYGLVKKVARTPALESLAVETSVMALPAVGYLLFVNAQGYGSFGHVSAGM"
     gene            complement(676..1236)
                     /locus_tag="VII50_11595"
     CDS             complement(676..1236)
                     /locus_tag="VII50_11595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5051537.1"
                     /translation="MMFLIDGGRSTTNQATLDWCNFTYTSEALRMARVQVEYTGNDSL
                     PAGNEFVHYQKGGTNRAWTEIEQAVSTCPPSSTTDGYVNDLVQRAPSDNELVARQLIL
                     SYHVTDPTGQLNLPWQAIVYQFDGDYFSGIYVYGLSRSLALAEAERLAAKSATHLAQA
                     SRGKPGTGGGPIQSVTSSSPPTGVQD"
     gene            1223..1504
                     /locus_tag="VII50_11600"
     CDS             1223..1504
                     /locus_tag="VII50_11600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5051538.1"
                     /translation="MRNIIELAPTSAGASRYWSSGPVALPGAVPAAGAVGCPVDGEAD
                     GEVGDDEGRDDCGYGEELGAEDGAGCGAPLTDGELDAGAGNAAGDRVDW"
     gene            complement(1738..>2290)
                     /locus_tag="VII50_11605"
     CDS             complement(1738..>2290)
                     /locus_tag="VII50_11605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="2-oxoacid:ferredoxin oxidoreductase subunit
                     beta"
                     /protein_id="HEY5051539.1"
                     /translation="SLALGAEATFVARTIDSDRKHLTAVLRAATEHRGTSLVEIYQNC
                     NIFNDDAFEVLKAPETRDDFLIRLEHGKPITFGTDAARGLRRSASGSLEVCDGNDPDV
                     LVHDAHDDDPSLAFALSRVTRPNLSMTPIGVFRDVQRPVYDDLMTAQLDQAVAKNGAG
                     DLVALLAGGDTWTITDTAPPAAN"
BASE COUNT          408 a          693 c          791 g          398 t
ORIGIN      
        1 tccatgccag ccgatacgtg cccgaacgat ccgtagcctt gcgcattgac gaagaggaga
       61 tacccgacag cgggcagcgc catcacggag gtctccacgg cgaggctctc gagcgcgggc
      121 gtgcgggcga ccttcttgac caggccgtag ccaccgaagc tcgctgcgag cgtgagcccg
      181 atccatggca cctgcccgta cccaacggcg atgaccagca ctgctacgac accgatgatg
      241 accgcgaccc attgtgttcg ttgcaggcgt tctttgagaa cgagcacgcc cagaccgatg
      301 ttgaccagtg gcgtgatgaa atagccgagc gacgactcga cgacgtggcc cgcattgacc
      361 gcccagatgt agacacccca gttgacgctg atgaggacgc cggcgagcac gagcaggccg
      421 acccgcttga ggtcatgccg gagctcgcca acgaacgccc atcgccgccg agctgccagc
      481 agcgtgccga tgaataccag tgaccacagc atgcggtggg cgaggatttg gatcgccggc
      541 gtcggatcaa gcagtggcca gtacaacggg aacaggcccc agatcgtgta cgcggcgagt
      601 ccgtatccaa atcctcggcg cgtatctcgc ataggagtaa ccgtagctgc aacgtttggc
      661 tgcggcctac cgagattagt cctggacccc agtaggggga gatgaagacg tcactgactg
      721 gatgggcccg ccaccggtgc cgggtttgcc gcggctggcc tgcgcgaggt gcgtagccga
      781 cttggcggcc agccgctcgg cctcagccaa cgccagcgat ctgctcagac cgtaaacgta
      841 gatgccagag aagtagtcgc cgtcgaactg gtacacgatc gcctgccacg ggagattcag
      901 ctggccagtc gggtcggtga cgtgatagct gagaatgagc tgccgcgcga cgagctcatt
      961 gtcggacggc gcgcgctgga cgaggtcgtt gacatagcca tcagtcgtcg atgagggcgg
     1021 gcacgttgag acagcttgtt caatctcggt ccatgctcgg ttcgtaccgc ccttttggta
     1081 gtgcacgaac tcgttgcctg cggggagcga gtcgttgccg gtgtactcga cctgcacccg
     1141 cgccatccgc aatgcctcgc tggtgtaggt gaagttgcac caatcgagag tcgcctggtt
     1201 ggtggtgctg cgccctccat cgatgaggaa catcatcgaa ctggcgccga cctccgcggg
     1261 agcgagcagg tactggtcga gtggacctgt tgcgctgccg ggtgcagtgc cggcggcggg
     1321 tgccgttggt tgcccggtcg acggcgaagc ggacggtgag gtcggtgatg acgagggtcg
     1381 cgatgactgc gggtacggcg aggagttggg cgccgaggac ggtgcgggtt gcggcgctcc
     1441 gctgacggat ggtgaactgg acgcaggagc gggcaacgcg gcgggcgatc gcgtcgactg
     1501 gtgattggcg tacaccacaa cgccgactcc cgctgccgcg agcacggcaa cgaccactcc
     1561 ggcgatcaaa cgcagcgtgc gcgatctttt gcgctccggt gggtctgggt ctcctagaga
     1621 tgcgccgaag gatgccgcag cagaagctcc cggaaccgcc cacggattaa cgggctgggc
     1681 accgtgttga ccctcgcggg accagtcgtc ggtcatggac tgtcgcgttg ccccgcgtta
     1741 gttcgcggca ggcggcgcgg tgtccgtgat ggtccacgtg tcgccgccgg ccagcagtgc
     1801 cacgagatcg ccggcgccgt ttttcgccac tgcctggtcg agttgcgcgg tcatgaggtc
     1861 gtcgtagacg ggccgttgca cgtcgcggaa cacgccgatc ggcgtcatcg acaaattcgg
     1921 tctggtgaca cgtgagaggg cgaatgccag cgacgggtca tcgtcgtgcg cgtcgtgcac
     1981 gaggacgtcg ggatcgttgc cgtcacagac ttcgagagaa ccggacgccg atctgcgcag
     2041 accgcgcgcc gcgtcggtac cgaaggtgat cggcttgccg tgctcgagcc ggatcaggaa
     2101 gtcatcccga gtctcgggcg ctttgagaac ttcgaacgcg tcgtcgttga agatgttgca
     2161 gttctggtag atctcgacga gcgacgtgcc gcggtgctcg gtcgccgcgc gcagcaccgc
     2221 agtcaggtgc ttgcggtcgg agtcgatcgt gcgcgcgacg aatgtcgcct cggctcctaa
     2281 cgccaacgag
//
LOCUS       DATCBJ010000578         2906 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_87225, whole genome shotgun sequence.
ACCESSION   DATCBJ010000578 DATCBJ010000000
VERSION     DATCBJ010000578.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2906)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2906)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2906
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_87225"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1099
                     /locus_tag="VII50_11610"
     CDS             <1..1099
                     /locus_tag="VII50_11610"
                     /inference="COORDINATES: protein motif:HMM:NF024739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY5051540.1"
                     /translation="APIACAGALAIAAMSTLGAASGAVNWSNATSAQAGGGFAALVAA
                     AKKEGTLNLIADPLNWANYGEIISSFSKKYGIKVNDVNKLGTSQQEITALQTLGSQSR
                     APDAVDVGQSYALNNASLFAPYKVQTWNMIPGANKAANGTWANDYGGYVSIGCNTSIV
                     KVCPTSFADLTNPAYKNDVALDDPPTTANSAFSGVWAAALANGGSFDNITPGITFFQK
                     LHQIGNFVETPGLAGAITAGQTPIVIDWDYNNAATAVANKGKFKWTTAVPTDASFAAF
                     YAQALSAKAPHPAAARLWLEYLYSATGQNLWLKGLSRPVELPAMIKNGTVNKTYLKAL
                     PAVAKPAKYPSLAQVTAAEAVVAQQWASSQS"
     gene            1261..1989
                     /locus_tag="VII50_11615"
     CDS             1261..1989
                     /locus_tag="VII50_11615"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEY5051541.1"
                     /translation="MTWFNFSQTFHQPYVGAFEESLKLALLTSLIGGVFGLWLACAVV
                     TAGPRSPLRRIVGSGAGVLAYFAGVPLAFAFIAALGRYGEATKLLQDIGINLNAGSFT
                     ISSLTGIFLCYVYFQIPLMVLLIIPALEGLRPEWHEAATGLGASTFGYIRHVAAPVLA
                     PALIGAVLLLFGNAFAAYATAYALVGSSVNLVPGWIQQLISGNVLVDENGIGLALGVE
                     MIVVIAIVMVGYAAVQRRATRWLQ"
     gene            2118..2798
                     /locus_tag="VII50_11620"
     CDS             2118..2798
                     /locus_tag="VII50_11620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5051542.1"
                     /translation="MGNYTQLFHDSALGSSLVTSLEIAALTTVIMLALMLPTVVWVRL
                     RLPRLAVVVESISVLPIVIPPIVMAAGLATLQSHASAQISSLVFTSPVTALTPFYVIL
                     AMPFAFRSLDVGVRAIDLRTLVDAARNLGAGWTTLLLRVILPNVRTAILTAASLTVAL
                     VLAEVVIAETLLYDTYPVELVNTGQNLVGVAVALSVLSLAVTWLLLYMVSFIGNRGAS
                     RARIGRLL"
BASE COUNT          465 a          993 c          910 g          538 t
ORIGIN      
        1 cgccccgatc gcctgcgcag gtgcgctggc gatcgcagcc atgtcgacct tgggcgccgc
       61 gtccggcgcc gtcaattgga gcaacgcaac ctcggcgcag gccggcggag ggttcgccgc
      121 cctcgtcgcg gccgccaaga aggagggaac gctcaacctc attgcggacc cgttgaactg
      181 ggccaactac ggcgaaatca tctcgtcgtt ctcgaagaaa tatggcatca aggtcaacga
      241 cgtcaacaag ctggggacta gccagcagga gatcaccgcg ctccagaccc tcggctcgca
      301 aagccgcgct ccggacgccg tcgacgtcgg acagtcctac gcgctcaaca acgcgtcgct
      361 gttcgctccg tacaaggtgc agacgtggaa catgatcccc ggcgcaaaca aggccgccaa
      421 cggcacctgg gccaacgact acggcggata cgtatcgatc ggctgcaaca cgtcgatcgt
      481 caaggtgtgc ccgacatcgt tcgcggatct gaccaatccg gcctacaaga acgacgtcgc
      541 gctcgatgat cctccgacga ccgcgaactc cgcgttctca ggggtatggg cggcagctct
      601 ggccaacggt ggctccttcg acaacatcac gccggggatc acgttcttcc agaagctgca
      661 ccagatcggc aacttcgtcg agaccccggg acttgcgggc gcgatcacag ccggacagac
      721 gccgatcgtg atcgactggg actacaacaa cgccgcgacc gcggttgcga acaagggcaa
      781 gttcaagtgg accacggcgg tcccaaccga tgcttcgttc gccgcgttct acgcgcaggc
      841 gctgagtgcc aaggcgcctc acccggcggc ggcccggctg tggctggagt acttgtactc
      901 cgcgaccggc cagaacctct ggctcaaggg cctctcgcgc cccgtcgagc tgccggcgat
      961 gatcaagaac ggcacggtca acaagaccta cctcaaggcc ctgccggcgg tcgcgaagcc
     1021 agctaagtac ccgtcactgg cgcaggtgac cgcggccgag gccgtcgtcg cgcagcagtg
     1081 ggcgtcgtcg cagtcctaga cgaagcggtc cctcagggtc ccgcggcgcg cacgcgtcgc
     1141 gggacccttc ggggtggcgc gtgggtgggg atcgcgccct tcgccgtgta cgtcgcgctg
     1201 tttctcgggg gtccgctcta tgaggtgatc tacggtgccg tgacgacaac acacggctcc
     1261 ttgacctggt tcaacttcag ccagacgttc caccagccct acgtcggggc tttcgaagag
     1321 agcctcaagc tggcgctgct gacatcgctg atcggcggcg tcttcggcct ctggctcgcc
     1381 tgcgccgtcg tgaccgcggg gccccgcagc ccgctgcgcc ggatcgtcgg atccggagcc
     1441 ggcgtgctcg cgtacttcgc gggggtcccg ctcgcgttcg ccttcatcgc ggctctcgga
     1501 cgctacggcg aggccaccaa actgctgcag gatatcggca tcaacctcaa tgccggcagc
     1561 ttcaccatca gttcgctgac aggcatcttc ctgtgctacg tctacttcca gatcccgctg
     1621 atggtgctgc ttatcatccc ggcccttgag gggttgcgac ccgaatggca cgaggcggca
     1681 accgggcttg gcgcatccac gttcggctac atccgccatg tggccgcgcc ggtgctcgcg
     1741 ccggcattga tcggcgccgt gctgctgctg ttcggcaacg ccttcgccgc ctacgcgacg
     1801 gcctatgcgc tggtcgggtc aagcgtcaac ctcgtgccag gctggataca acagctgatc
     1861 tcgggcaacg tgttggtcga cgagaacggc atcggcctgg ctctgggcgt cgagatgatc
     1921 gtcgtgatcg cgatcgtcat ggttggctac gctgcggtcc aacgccgcgc cacgcggtgg
     1981 ctgcagtgag acggcgccgc cggcggcggc cacgcctgtg gcgggtgacg attctcgtgc
     2041 tcgccgcggt tttctttctc ggtccgcagg tctccgcgtt gagatacagc tttgttcaga
     2101 gcaatggaag gaacggtgtg ggcaactaca cccagctgtt ccatgactcc gcgctgggca
     2161 gctcgctcgt gacttcgctc gagatcgcgg cactcacgac ggtgatcatg cttgcgctga
     2221 tgctgccgac cgtcgtctgg gtgcgcctgc gcctgccccg tctggcggta gtggttgaga
     2281 gcatcagcgt attgccgatc gtaatcccgc cgatcgtgat ggccgctgga ctggcgacgc
     2341 tccagtcgca cgcgtcggcc cagatctcca gcctcgtctt cacttcgccg gtgaccgcgc
     2401 taaccccgtt ctatgtcatc ctcgcgatgc cattcgcctt tcgctcgctc gatgtcgggg
     2461 tgcgagcgat cgacctacgc acgctcgtcg acgcagcgcg caacctcggc gctggctgga
     2521 cgacactgct gctgcgcgta attctcccca acgtgcgcac cgcaatcctc accgctgcct
     2581 cgctgaccgt cgcactcgtg ctcgccgagg tcgtcatcgc agaaacgctg ctgtatgaca
     2641 cctacccggt ggagctcgtc aacaccggtc agaacctggt cggcgtcgcg gtcgcgcttt
     2701 cggtgctctc gctcgccgtc acctggctgc tcctgtacat ggtttccttc attggcaacc
     2761 gcggtgcgtc aagagcgagg atcggcaggc tactgtgagc gccgtcgcgg tacatctgga
     2821 tcgcctgacg cggcgcttcg gcgcgctcac cgccctcgac gggctggatc tcgatgtcgc
     2881 ccctggcgaa ctgctggccc tgctcg
//
LOCUS       DATCBJ010000579         2590 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_435468, whole genome shotgun sequence.
ACCESSION   DATCBJ010000579 DATCBJ010000000
VERSION     DATCBJ010000579.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2590)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2590)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2590
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_435468"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..738
                     /locus_tag="VII50_11625"
     CDS             <1..738
                     /locus_tag="VII50_11625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5051543.1"
                     /translation="SATTGFAVADSAPNGANGELMTTSDAGTTWHWLPSPSGLQTVCI
                     DSSGRGWLGANGDIYGTTDRGAHWTRAFEQPTDGNGINPYMVDLQCAGQGAAWAEMLG
                     GGAMSKQAHLGLHTGGLTWSAIFAEQFFPHPGVNVDADAPSSYFGAFSAVSPTTAVFV
                     DNCVACGYGQPWIETTSDGGAHLSKRDVVTNLTDATAASFVTVTDGWVIGIDQTADQG
                     GNAGSTFLRIEHTADGGRTWATQYSTQ"
     gene            845..2536
                     /gene="paaN"
                     /locus_tag="VII50_11630"
     CDS             845..2536
                     /gene="paaN"
                     /locus_tag="VII50_11630"
                     /inference="COORDINATES: protein motif:HMM:TIGR02288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetic acid degradation protein PaaN"
                     /protein_id="HEY5051544.1"
                     /translation="MPDADALIATHAPLLAQAREAAHARTYFAAFAESPSTRIWGEAA
                     PADGRAAFDALLHQTFPILTPGAVGSIAPEEPPYGLALDVSYPHLHADGVNELMSVAK
                     KAMPAWRDAGPELRTALGIEVLTRINARSFEFAHAVMHTTGQAFVMAFQAGGAHARER
                     GLEAIAYAYDAMTQVPASVSWEKPRSKGEPQRMAKTYTIVPRGVALVIGCSTFPTWNS
                     YPGFFASLVTGNPVIVKPHPHAVLPLALTVGVARDVLTEYGFDPNLITLAVDGAGETV
                     ASTLAMHPDVRVIDYTGSTSYGEWLETNALQAKVFTEKAGVNCVVLDSTDDLNGLAAN
                     LAYSLSLYSGQMCTTPQTIFIPRGGINTDRGHTSVVDVVTALSSALDDLLGEPARAVN
                     MLGAIVDDAVVRRLDGASTAGEVLIKSRAIEHPEFPAARVRTPLVVRAASDVHAYSHE
                     CFGPIAFIVETADTAASLDGWSRIVTASGSLTASVYSTDAGVLTAAHNVALDTGVSMS
                     SNLTADIYVNQTVAFSDYHGTGSNRAANATLTDLAFVADRFRIVETRHPAVNPAQ"
BASE COUNT          468 a          900 c          816 g          406 t
ORIGIN      
        1 agcgcgacca ccggtttcgc cgtcgccgac agcgcaccga acggcgccaa cggcgagctc
       61 atgacgacat cggacgccgg cacgacctgg cactggttgc catcgccgtc cggcttgcaa
      121 accgtttgta ttgacagttc gggccgcggc tggctcggcg cgaacggcga catctacggc
      181 accaccgacc gcggcgcgca ctggacacgt gccttcgaac aaccgaccga cgggaacggg
      241 atcaacccgt acatggtcga cctgcagtgc gccggccagg gtgctgcgtg ggccgagatg
      301 ctcggaggcg gcgctatgag caaacaagca cacctcgggc tacacaccgg cggcctgacg
      361 tggtcggcga ttttcgcgga gcagttcttc ccgcacccgg gagtcaacgt tgatgccgac
      421 gcgccaagct cctacttcgg tgctttcagc gccgtcagcc caaccaccgc ggtgttcgtc
      481 gacaactgcg ttgcctgcgg gtacggccag ccgtggatcg agacgacgtc cgacggcggc
      541 gcgcacctga gcaagcgcga cgtggtcacc aacctgactg atgccaccgc cgcaagcttc
      601 gtcaccgtca ccgacggctg ggtgatcggc atcgaccaga ccgcggatca gggcggcaac
      661 gctgggtcca cgttcctgcg cattgagcac accgcggacg gcgggcgcac ctgggcgacg
      721 cagtactcaa cgcagtagcg gtgggctcgg agcttccgtc tcgaccgaac gttcggttta
      781 ctagccttac cggaggccgt tccgcgacgc gggcgaccag gacacgcggg aggcgctcgt
      841 cacgatgccc gatgctgacg cactgatcgc gacccatgcc ccgctgctgg cacaggcccg
      901 cgaggctgcg cacgcccgca cgtacttcgc cgcctttgcc gagtcgccct cgacccgcat
      961 ctggggcgag gccgcgccgg cggacggcag ggccgccttc gacgccctcc tccaccagac
     1021 cttccccatc ctcacgcccg gagcggtcgg atcgatcgca cccgaggaac cgccctatgg
     1081 cttggcgctc gacgtcagct acccgcacct gcacgccgac ggcgtcaacg agctcatgag
     1141 cgtggccaag aaggcgatgc cggcctggcg cgatgcaggc ccggagctcc ggaccgcgct
     1201 cggcatcgaa gtcttaaccc ggatcaacgc gcggagcttc gagttcgcgc acgccgtgat
     1261 gcacaccacc gggcaggcat ttgtgatggc cttccaggcc ggcggcgcgc acgcacgcga
     1321 acgcgggctc gaggcgatcg cctacgcgta cgacgccatg acgcaagtgc cggcgtcggt
     1381 gagttgggag aagccccgta gcaaaggcga accacagcgg atggccaaga cgtacacgat
     1441 cgtgccgaga ggggttgcgc tcgtcatcgg ctgttcgaca tttccgacct ggaacagcta
     1501 tcccggcttc ttcgcgagcc tggtgaccgg caaccccgtc atcgtcaagc cgcatccaca
     1561 tgccgtactt ccgctggcac tgacagtcgg cgttgcccgc gatgtgctga cggagtacgg
     1621 cttcgacccg aacctgatca cactcgccgt cgatggcgct ggcgaaaccg tcgcgagcac
     1681 cctcgcgatg catccggacg tgcgcgtcat cgactacacc ggatccacgt catacggcga
     1741 gtggctcgag acgaatgcgt tacaggcgaa ggttttcacc gagaaggccg gcgtgaactg
     1801 cgtcgtcctc gactccaccg acgacctgaa cggtctcgcc gcgaacctcg cctattcact
     1861 gtcgctgtac agcggtcaga tgtgcacgac accacagaca atcttcatcc cacgcggcgg
     1921 catcaacacg gatcgcgggc acaccagcgt cgtcgacgtc gtcactgcac tcagtagtgc
     1981 gctcgacgac ttgctgggtg agcccgctcg tgcggtcaac atgctcggcg cgatcgttga
     2041 cgacgccgtt gtgcgccgtc tcgacggggc gtcgaccgcg ggcgaggtgc tgatcaagtc
     2101 gcgggcgatc gagcacccgg agttcccagc cgcacgggtc cggacgccac tagtcgtgag
     2161 agccgcatcc gacgtacatg cctacagcca tgaatgtttc ggccccatcg ccttcatcgt
     2221 ggagacagca gacaccgcag ccagcctcga tggctggagc cggatcgtga cggccagcgg
     2281 atcgttgacg gcatcggtct actcaacgga cgccggggtg ctgacggcgg cgcacaacgt
     2341 ggcgctcgat accggcgtgt cgatgtcgag caacctcacg gctgacatct acgtgaatca
     2401 gacggtcgcc ttctccgact accacggcac cggctcgaat cgagctgcga acgcaacact
     2461 gactgatctg gcgttcgtcg ccgatcgatt ccggatcgtc gaaacccgac accccgcagt
     2521 gaatccagcc cagtgaagcc ggccgtagga acggtggctg ctgagcggcg tccggcccca
     2581 atacctcggc
//
LOCUS       DATCBJ010000580         2367 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_3488, whole genome shotgun sequence.
ACCESSION   DATCBJ010000580 DATCBJ010000000
VERSION     DATCBJ010000580.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2367)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2367)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2367
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3488"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..180)
                     /locus_tag="VII50_11635"
     CDS             complement(<1..180)
                     /locus_tag="VII50_11635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5051545.1"
                     /translation="MAARSLLRCGAIGGAIAVAVVGSAVLAAGPALAIGERGDPSGKL
                     SAIETAAIFVGIPVGV"
     gene            complement(294..>2367)
                     /gene="malQ"
                     /locus_tag="VII50_11640"
     CDS             complement(294..>2367)
                     /gene="malQ"
                     /locus_tag="VII50_11640"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HEY5051546.1"
                     /translation="VSVLAALGVDASTPEAAREALERARHGRWRDMLPSYLVVRGGAT
                     ARVAVHVPHGEAVTVDLEVEGGGRIPLTQLMIWVDPHDVDGELIGEATFEIPDVVPLG
                     YHYLHAQSGQRSAKAQLIVVPDQLVVPAGRHWGFMLQLYAVRSHASWGMGDLRDLADL
                     VTWSGRDLGADFVLVNPLHAAGPVGPMEPSPYYPSSRRFVNPIYLRVDDVPEVAALPP
                     EEVEAVARVLRDRNELDEDLDRDAVWSAKRQILERAFAALDDDGRRAERDEFLVAEGD
                     ELRDFATWCALVDRHGAATRDWPEELRDPHSTKVAAFRDAEAKLIEFHAWLQFLCSQQ
                     LTEVQRAARGAGMSMGVMNDLAVGVSPEGADAWAFHGALAHGVALGAPADMYNQQGQC
                     WNLPPWRPDALAAAGFAPFRDLVRASFRHGGGLRIDHIIGLFRQWWVPEGASPADGTY
                     VRLDHEAMVGIVLLEATRAGAVLVGEDLGNVEWWVRDYLRARGVYGTSILWFEHDDVG
                     RPRPPDQWRELCLASVTTHDLPPTAGYLTGEHVELRHELGLLERPVEEERRSDEAGRV
                     AWLGALVGAGLLDVGVVAAVTKVAEPGTVSPRRHNFRTRVDPYVDRIVEALYAYLALT
                     PARLIGIYLPDVVGDRRPVNQPGTIDEYPNWRVPMADEKERPVLLEELMTSPVASRLA
                     RLVTGSDA"
BASE COUNT          415 a          861 c          737 g          354 t
ORIGIN      
        1 gacgcccacc ggaatgccaa cgaagatcgc ggccgtctcg atcgcgctga gcttgccact
       61 cggatcaccc cgttcgccga ttgcgagcgc cggcccggcc gcgagcaccg cagacccgac
      121 gacggcaacg gcaatggcgc cgccgatcgc cccgcatcga agtaacgacc tagcagccac
      181 tagcagtcga ggcgtcctca cagcggccac cgtaacggat acgggtggca caatcctcat
      241 gcgggttcac cccgtgcctc atgcgggttc accctgtggc gccacgctgg ggttcacgcg
      301 tcggagccgg ttacgagccg cgccagccga ctggcaaccg gcgaggtcat cagttcctcg
      361 agcagtaccg gccgttcctt ttcatctgcc atcggcaccc gccaattcgg atactcgtca
      421 atagttccag gctggttcac cgggcgccgg tcgccgacga cgtccggcag gtagatgccg
      481 atcaaccggg ccggcgtcaa agccaggtag gcatacagcg cctcgacgat gcggtcgacg
      541 taaggatcga cccgcgttcg gaaattatgg cggcggggcg acaccgtccc cggctccgcg
      601 accttcgtga cggccgccac gacgcccacg tcgagtagtc ccgcgccgac gagtgcgccc
      661 aaccacgcaa cccgccccgc ctcgtcgctc cggcgctcct cctcgaccgg gcgctcgagc
      721 aggccaagct cgtggcgtag ttcgacgtgc tctcccgtga ggtatccagc agtcggcggc
      781 aaatcgtgtg tcgtcacgct ggcaaggcac agctcgcgcc actggtcggg tggtcgcggc
      841 cggcccacgt cgtcgtgttc gaaccacagg atcgacgtgc cgtagacccc acgagcgcgc
      901 aggtagtcgc gtacccacca ttcgacgttg cccagatcct caccgaccag cacggcgccg
      961 gcccgcgtcg cctcgagcag cacgatgccg accatcgcct cgtggtcgag gcgcacgtac
     1021 gtgccgtcgg ctggcgatgc gccttcaggc acccaccact ggcggaacag gccgatgatg
     1081 tggtcgatgc gcaggcctcc gccgtgccga aacgatgccc ttaccagatc gcgaaacggc
     1141 gcgaaccccg ctgcggccaa cgcgtcggga cgccacggcg gcaggttcca gcactggcct
     1201 tgctggttgt acatgtcggc cggcgctccc aatgcgacac cgtgcgcgag cgcaccgtgg
     1261 aacgcccatg cgtcggcgcc ttcagggctg acgccgacgg caaggtcgtt catcacgccc
     1321 atactcatgc cggcgccgcg cgcggcccgc tgcacctcgg tcagctgttg cgagcacaga
     1381 aactgcagcc aggcgtggaa ttcgatgagc ttggcctcag catcacggaa cgcggcaacc
     1441 ttcgtcgaat gcgggtcccg cagttcctcc ggccaatcgc gagtggctgc tccgtgccgg
     1501 tcgaccaacg cacaccacgt ggcgaaatca cgaagctcgt caccctcagc aacgagaaac
     1561 tcgtcgcgtt ccgcacgtcg gccgtcgtcg tcgagagcgg cgaacgcgcg ttcgagaatc
     1621 tgccgtttcg ccgaccacac ggcgtcccgg tcgaggtctt catcgagctc gttgcgatca
     1681 cgcagaacgc gcgcgacggc ctcgacttct tccggcggga gcgcggccac ctcgggcacg
     1741 tcgtcgactc gaaggtagat ggggttcacg aagcgccggc tcgacgggta gtagggcgac
     1801 ggctccatcg gcccaaccgg accggccgca tgcagcggat tgaccagcac gaagtcggca
     1861 ccaagatcgc gcccgctcca cgtgacgaga tcggcgagat cgcgcaggtc acccatgccc
     1921 cacgacgcgt gcgaccgcac cgcgtacaac tgcagcatga atccccagtg ccggcccgca
     1981 ggcacaacaa gttgatccgg gacgacgatc agctgcgctt tcgcgctgcg ctgccccgac
     2041 tgcgcgtgca ggtagtgata accgagcggc acgacgtccg gtatctcgaa cgtcgcctcg
     2101 ccgatcagct ccccgtcgac gtcgtgcgga tcgacccaga tcatcaattg cgtcaaggga
     2161 atccggccgc caccttcgac ctcaaggtcg acggtcacgg cctcaccgtg cggcacgtgc
     2221 acggcaaccc tcgccgttgc gccaccgcgc acgacaaggt aactcggcag catgtcgcgc
     2281 caccggccat ggcgcgcgcg ttcgagggcc tcacgtgccg cttcgggtgt gctcgcgtcg
     2341 acaccgagtg ccgcgagcac cgagacc
//
LOCUS       DATCBJ010000581         3497 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_364198, whole genome shotgun sequence.
ACCESSION   DATCBJ010000581 DATCBJ010000000
VERSION     DATCBJ010000581.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 3497)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3497)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3497
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_364198"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(77..526)
                     /locus_tag="VII50_11645"
     CDS             complement(77..526)
                     /locus_tag="VII50_11645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TOBE domain-containing protein"
                     /protein_id="HEY5051547.1"
                     /translation="MSTYRIADAARLLGVSDDTARRWAESGRFASTTDESGRLAIEGA
                     ELARFATTLSDTLEPGQVVQASARNRLVGIVTKVTRDTVMAQVEMQCGGHRVVSLMSR
                     EAADELELEPGVLAIASIKSTNVVVELPAPLTGRRPAGVPANSSESQ"
     gene            complement(592..1368)
                     /locus_tag="VII50_11650"
     CDS             complement(592..1368)
                     /locus_tag="VII50_11650"
                     /inference="COORDINATES: protein motif:HMM:NF014019.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HEY5051548.1"
                     /translation="MTEGLDQTAQTRLAESGKFFTSDLSVAEFTLLEHTGFDAIELVM
                     GVSVFHVGFQPGRGMQPGEYQVLSQAMYTARMNAINRLIAETVKVGGDGVVGVKLGYR
                     AHSADALEFTAIGTAVRHRDGTASHRQPSGAPFTSHLDAKELYQLLNVGWCPVSFVLG
                     TCVYHIAVQGVMQTMRQVGQNIELPQWTQGFYDAREIAMTRLQAEGERDGAQGVVGVT
                     VTTSEWVWGGHTLEFYVSGTSVRRVSDQRGQLPPLVVPVG"
     gene            complement(1429..1596)
                     /locus_tag="VII50_11655"
     CDS             complement(1429..1596)
                     /locus_tag="VII50_11655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5051549.1"
                     /translation="MPDHDGTDEKELEAEAKAHIDEMPAETALGNADPEGGGKGTPEE
                     FVAEHEGRGGK"
     gene            complement(1668..2516)
                     /locus_tag="VII50_11660"
     CDS             complement(1668..2516)
                     /locus_tag="VII50_11660"
                     /inference="COORDINATES: protein motif:HMM:NF012976.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0018576 - catechol 1,2-dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0009712 - catechol-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dioxygenase"
                     /protein_id="HEY5051550.1"
                     /translation="MTDEVVASFAGAQSERYRQVMQSLVRHLHDFVRDVRLTDEEWHE
                     AVGFLTRTGHMCDDRRQEFVLLSDVLGLSMLTVAVNEIPGSSATQSTVFGPFFVETSP
                     EIAQGGDLAAGAAGQPCWVEGTVRGVGGEPVAAARIDVWEADGDGLYDVQYDGARTAN
                     RGHLLTDAGGGFRFWSVRPVAYPIPSDGPVGRLLAAAGRGAMRPAHIHFMIRAAGYHK
                     LTTHVFAAGDEYLRDDAVFGVKEQLVAEFIDHPATEAPPRGGPAGSDWSSVSYDFVLA
                     SAVEPG"
     gene            2667..>3497
                     /locus_tag="VII50_11665"
     CDS             2667..>3497
                     /locus_tag="VII50_11665"
                     /inference="COORDINATES: protein motif:HMM:NF024118.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEY5051551.1"
                     /translation="MTGDAVDPIALEPVDEVLVTTLVDNSFDALLPGDDRVSRTSFIG
                     QVAAEQFEGGRTSVGLRAEHGFSALVTVRRAGTTTSLLFDTGISPDGMTCNAHRLGVD
                     LNQIQGVVLSHGHFDHAGGLSGLAAMRGGRLPMTVHPLIWTRRRLVVPGVDPFDMPTL
                     SKRALEGEGFEVVERRQPSLLVDRSVLITGEIDRTTEFERGMPPTHQAWDGEQWRHDP
                     LVLDDQALVVHVRGKGLVVLTGCGHGGAVNIVRHAMRLTGISRLHALIGGLHLSGAAF
                     E"
BASE COUNT          589 a         1170 c         1148 g          590 t
ORIGIN      
        1 ttcttcgacg agctgcacgc agtgagacct agcgcgatga cgacggcggt gacgatgacg
       61 gcggagacat ggcggttcat tgactctccg aggagtttgc agggacgccg gctggtcggc
      121 gtccggtgag aggggccgga agctccacca ccacgttggt tgacttgatc gaagcgatcg
      181 ccaggacgcc tggctccaac tccaactcat cggcagcctc gcggctcatg agggagacga
      241 cccgatgacc gccgcactgc atctcaacct gggccatgac ggtgtcgcga gtcactttgg
      301 tcacgatccc gaccaagcgg ttgcgcgctg acgcctgcac cacctgcccc ggctcgagcg
      361 tgtcgctcaa ggtcgtcgcg aaccgcgcga gctcggcgcc ctcgatcgcg aggcggcccg
      421 actcgtcagt cgttgacgcg aacctgcccg actcggccca acgccgcgcg gtgtcgtcgc
      481 tgacgccgag aaggcgtgcg gcgtcggcga tccgatacgt ggacacgcgc caacattaac
      541 cgcagaagcg gcgccagacc agcgaagatc cccgcagatg cggaaaacga gctagccgac
      601 ggggacgacg agcggcggca actggccgcg ctgatcgctg acgcggcgca ccgacgtccc
      661 ggatacatag aactccagcg tgtggccgcc ccagacccac tcgctcgtgg tgaccgtgac
      721 gcccacgaca ccttgggcgc cgtcgcgctc gccctcggct tgcagtctgg tcatcgcaat
      781 ctcgcgggcg tcgtagaagc cctgggtcca ctgggggagc tcgatgttct ggccgacctg
      841 ccgcatcgtc tgcatcacgc cctgcaccgc gatgtggtag acgcaggtgc cgaggacgaa
      901 cgagaccgga caccaaccca cgttgagaag ctggtaaagc tccttcgcgt cgaggtgact
      961 ggtgaacgga gcgccggacg gttgccggtg cgaggcggtg ccgtcgcggt gacgaactgc
     1021 ggtgccgatc gcggtgaatt caagggcgtc ggcgctgtgc gcgcgatagc cgagcttgac
     1081 cccgaccacg ccgtccccgc cgaccttcac ggtctccgcg atcagtcgat tgatggcgtt
     1141 catccgcgcc gtgtacatcg cttgcgacag gacctggtac tcaccaggct gcatgccgcg
     1201 accgggttgg aagccgacgt ggaaaacgga cacgcccatc acgagctcga tcgcgtcgaa
     1261 cccggtgtgc tcgagcaagg tgaactcggc aaccgacagg tcggacgtga agaacttccc
     1321 ggattccgcg agccgggtct gcgctgtctg atcgagacct tcagtcatgg tgatcaagct
     1381 agcaacgcac gactaccgat caccagatgc cactcgccaa ccgaggccct acttgccgcc
     1441 gcgcccttcg tgctcggcga cgaactcctc cggagtgcct ttgccgccgc cctcagggtc
     1501 ggcgttcccg agcgcagtct ccgcgggcat ctcgtcgata tgcgccttcg cctccgcctc
     1561 gagttccttc tcgtcggttc catcgtggtc gggcatgggc gctcctcgtc ctcttggtgt
     1621 gtgttcacct gctacccgcg aatctaccca cgcaaccagg accgcgctta gccaggttcg
     1681 acggcggacg ccagcacgaa gtcgtagctc acgctcgacc aatccgatcc cgccgggccg
     1741 cctcgcggcg gcgcctcagt cgcagggtgg tcgatgaact cggcaaccag ctgctccttg
     1801 actccgaaga ccgcatcgtc tcgcaggtac tcgtcgccgg cggcgaagac gtgcgtggtg
     1861 agtttgtggt aacccgcagc gcggatcatg aaatggatgt gcgcgggtcg catcgcgcca
     1921 cggccggcgg ccgcgagaag gcgcccgacc gggccgtcgc tcgggatcgg gtacgcgacg
     1981 ggccgaaccg accaaaagcg aaagccgccc ccggcgtcag tgaggagatg cccgcggttc
     2041 gcggtacggg cgccgtcgta ttggacgtcg tacaagccat cgccgtccgc ttcccacacg
     2101 tcgatcctgg cagctgcgac cggctcgccg cctacgccac gaacggtgcc ttcaacccag
     2161 cacggctgac ccgcggcgcc cgcagccagg tcgccgccct gtgcaatctc cggggaagtc
     2221 tcgacgaaga acggtccaaa gacggttgac tgcgtggctg acgaaccggg gatctcgttc
     2281 acggcgacgg tcagcatcga gagtccgaga acgtcggaga gcaacacgaa ttcctgccgc
     2341 cggtcatcgc acatgtgtcc ggtgcgggtg aggaaaccga cggcctcgtg ccactcctca
     2401 tcggtcagcc ggacgtcgcg cacgaagtcg tgtaggtggc gcacgaggct ttgcatcacc
     2461 tggcggtagc gctcggactg cgcgccagcg aagctggcca cgacctcgtc ggtcacggca
     2521 agctctcgag cctcttgcga cgcagacgtc atcaggagag cgagcctaat cccgcagccg
     2581 tcgacatgcg ccaggtcggg gaatatcgtc cgcgcatgtg cgcgacacct ggcaccattg
     2641 acgcccttgc ctcccagccc cggctcgtga ccggcgacgc tgtcgacccg atcgccctgg
     2701 agcccgtcga cgaagtactc gtgacgactc tggtcgacaa ctctttcgac gctttgcttc
     2761 ccggcgacga tcgggtgagc cggacctcat tcatcggcca ggtcgcggcc gagcaattcg
     2821 agggaggtcg gacgtcggtt ggtctgcgcg cggaacacgg cttctctgcg ctcgtgacag
     2881 ttcgccgcgc tggcaccacg acgtccctgc ttttcgatac cggcatttca ccggacggta
     2941 tgacttgtaa cgcccaccgg ctcggcgtcg acctcaacca gattcagggg gtggtcctca
     3001 gtcacgggca tttcgatcac gccggggggt tatcgggtct ggccgcaatg cgaggcggcc
     3061 ggctgccgat gacagttcac ccgctcatct ggactcgccg ccgactggtc gtgccgggtg
     3121 tcgatccgtt cgacatgccg acgcttagca agcgggcgct ggagggcgag ggtttcgagg
     3181 tcgtcgagcg gcggcagccg tcgttgctcg tcgatcgcag tgtcctcatt accggcgaga
     3241 tcgatcgcac caccgagttc gaacgcggga tgccgcccac gcatcaggcg tgggacggcg
     3301 agcagtggcg gcacgacccg ttggtgctcg atgaccaggc actcgtcgtc catgttcgag
     3361 gcaaggggct tgtcgtgttg acggggtgtg ggcacggcgg cgcggtgaac atcgtgcggc
     3421 acgcgatgcg gctgaccggg atatcccggc tgcatgcgtt gatcggcggc ctgcatctga
     3481 gcggggccgc gttcgag
//
LOCUS       DATCBJ010000582         2496 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_459451, whole genome shotgun sequence.
ACCESSION   DATCBJ010000582 DATCBJ010000000
VERSION     DATCBJ010000582.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2496)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2496)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2496
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_459451"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..379
                     /locus_tag="VII50_11670"
     CDS             <1..379
                     /locus_tag="VII50_11670"
                     /inference="COORDINATES: protein motif:HMM:NF024273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEY5051552.1"
                     /translation="EQVTTEPIEGEWSVAEITGHLFDVDLVYGFRWRLMLTEDNPSYP
                     GYDEKLWTPMPRLPFPQLLEAWAGLRAANMLVLQANWPAAAQRSANHSQQGPETFDVT
                     LRKIAAHDLAHLNQMQRAAEASA"
     gene            433..1008
                     /gene="dcd"
                     /locus_tag="VII50_11675"
     CDS             433..1008
                     /gene="dcd"
                     /locus_tag="VII50_11675"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012850633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009394 - 2'-deoxyribonucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HEY5051553.1"
                     /translation="MLLSDRDIRAELAAGRVVLDPYDPIMVQPSSVDVRIDKYFRVFE
                     NHRYPHIDPAVEQPDLTRLVEPAADEPFILHPGEFVLASTYEVITLPDDVAGRLEGKS
                     SLGRLGLLTHSTAGWIDPGFSGHVTLELSNVATLPIKLWPGMKIGQLCLFRTSSPSEF
                     PYGSSVYGSRYQGQRGPTPSRSWQNFHRTQI"
     gene            1025..1390
                     /locus_tag="VII50_11680"
     CDS             1025..1390
                     /locus_tag="VII50_11680"
                     /inference="COORDINATES: protein motif:HMM:NF037170.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY5051554.1"
                     /translation="MAMRLGNVVFDSANPQAAAAFYSELTGWPIVRTDGDWIDLQTES
                     GAALSLQLAPDHVKPQWPDPTRPQQAHVDLIVDDMDAEHARALALGATLLDDASDHPT
                     FRVFADLDGHPFCLCSGSS"
     gene            complement(1567..2106)
                     /locus_tag="VII50_11685"
     CDS             complement(1567..2106)
                     /locus_tag="VII50_11685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5051555.1"
                     /translation="MEVVALADRPDLHHAIDAMPKSWPTFMYKDPAARLMSRLPSTFP
                     QLQFAALDGDEVVGIVIAASFAWDGDLTSLPDRGWDAILEQVIRDEDEGRMPTASPCS
                     KLPFTRPARRRLECLAADRRPRSLRGPRVRIPLRAGPAESQITRADGTDRRLRGAPAP
                     RRVAVRSVAARTSDSRRTS"
     gene            complement(2116..>2496)
                     /locus_tag="VII50_11690"
     CDS             complement(2116..>2496)
                     /locus_tag="VII50_11690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020663866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HEY5051556.1"
                     /translation="SVTYHDPCYLGRHNKVYGQPRDVLAAVPGLTSTEMPRCKERGFC
                     CGAGGARMWMEERVGKRINHERIDEALALDPDIVVTACPYCRVMLGDAVRDRQSQGSA
                     RADVVVIDVAEVLLRSVRGPGPDH"
BASE COUNT          437 a          868 c          786 g          405 t
ORIGIN      
        1 cgagcaagtg accaccgagc cgatcgaagg cgaatggtcg gtcgcggaga tcaccggcca
       61 tctcttcgac gtcgacctgg tctacggatt tcgctggcgg ttgatgctca ccgaagacaa
      121 ccccagctat cccggctacg acgagaagct ctggacgccc atgccgcgcc tgccgtttcc
      181 acagctcctg gaggcgtggg ccggtcttcg ggcagcgaac atgctggtgc tgcaggccaa
      241 ctggccggcc gccgcgcagc gcagcgcgaa ccactctcaa cagggtccgg agacgttcga
      301 cgtgacactg cgcaagatcg ccgcgcatga cctggctcac ctgaaccaga tgcaacgggc
      361 tgccgaagcc tccgcgtgat cggcggccga ctcgccggtg gcgcacgcct gcccgaccga
      421 tagattgcgc gcgtgctgct ctccgaccgc gacatcagag ccgagctcgc ggcgggtcgg
      481 gtcgtcctcg atccgtacga cccgatcatg gtccagccgt ccagtgtcga cgtccggatt
      541 gacaagtact ttcgggtctt cgagaaccac cgctacccgc acatcgatcc ggcggtcgag
      601 cagcccgacc tgacccgcct ggtcgagccc gccgccgacg agccgttcat cctgcacccg
      661 ggtgagttcg tgctcgcctc gacgtacgag gttattactt tgccggacga cgtagccggg
      721 cgtcttgagg ggaagtccag cctcggccgg cttgggctac tgactcacag caccgccggc
      781 tggatcgatc cgggattctc cggccacgtc accctcgagc tgagcaacgt ggcgactctg
      841 ccgatcaagc tctggccagg gatgaagatc ggtcagctgt gcctattccg tacctcctcg
      901 ccgtccgagt tcccgtatgg cagttctgtc tatggctcgc gatatcaagg acagcgcggg
      961 ccgacgccgt cccggtcgtg gcagaacttt caccgcaccc agatctgact caccggaggt
     1021 accgatggcg atgcgcctcg gaaatgtcgt cttcgacagc gccaaccccc aggcggcagc
     1081 ggcgttctat tccgaactca ccggctggcc gatcgtgcga acagacggcg actggatcga
     1141 cctgcagacg gagagtggtg ccgcgctctc gctgcagctc gcacccgatc acgtgaagcc
     1201 ccagtggcca gaccccacgc gaccacagca ggcccacgtc gacttgatag tcgacgatat
     1261 ggacgccgag cacgcccggg cgctcgcgct gggggcgacg cttctcgatg acgcgtccga
     1321 ccatccgacg ttccgggtgt tcgcggatct tgacggccac ccgttctgcc tctgtagcgg
     1381 cagcagctaa gcgagcgggt gctgcatcca gacgttcggc tcgacataga ccgcgtggtc
     1441 gtgctcgacc gatgggtgca ccggcaccaa cgcaccgggc accacaacat cgcctgagac
     1501 gtcgaaccgc aggccggtcc agtcacgcca gcgcgccgct gatcgtcatg gacgccggag
     1561 cgatcttcac gacgtgcgcc tcgagtcgct cgtgcgcgca gccacggatc gaacggcaac
     1621 ccgtcggggc gccggggcgc cacgtagtcg gcgatcggta ccgtcggctc gagtgatttg
     1681 tgactcggcc ggaccggctc gaagagggat ccgaacccga gggccgcgca gtgatcgcgg
     1741 tcgccggtca gcagccaggc actcaagccg ccgccttgcc ggccgggtga acggcagctt
     1801 cgagcaaggt gacgccgtcg gcatccggcc ctcgtcttca tcgcgaatga cctgctcgag
     1861 gatcgcgtcc caaccgcggt ccggcagcga ggtgaggtcg ccgtcccatg cgaaggaagc
     1921 ggcgatgacg ataccgacca cctcgtcgcc gtccagcgcc gcgaactgca gctgcgggaa
     1981 cgtcgacggc agccggctca tcaatcgagc cgccggatcc ttgtacatga acgtcggcca
     2041 cgacttaggc atcgcatcaa tggcgtgatg caggtccggt cgatcggcca gtgcgacaac
     2101 ttccaccggg tcgccctagt gatctgggcc cggcccgcgc accgaacgca gcagcacctc
     2161 ggcgacgtcg atcaccacga cgtccgcgcg cgcagaacct tgagactgcc gatcgcggac
     2221 ggcgtcgccg agcatgaccc ggcagtacgg gcaggccgtg acaacgatgt cagggtcgag
     2281 tgcgagcgct tcgtctatgc gctcgtgatt gatccgcttg ccgacgcgct cctccatcca
     2341 catccgggcg ccccccgcgc cacaacagaa tccgcgctcc ttgcagcgcg gcatctcggt
     2401 cgatgtcaga cccggaactg ccgcgagcac atcacgcggt tgtccgtaca ccttgttgtg
     2461 ccggccgagg taacacggat cgtgataggt caccga
//
LOCUS       DATCBJ010000583        10234 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_348246, whole genome shotgun sequence.
ACCESSION   DATCBJ010000583 DATCBJ010000000
VERSION     DATCBJ010000583.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 10234)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10234)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10234
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_348246"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..734
                     /gene="modA"
                     /locus_tag="VII50_11695"
     CDS             <1..734
                     /gene="modA"
                     /locus_tag="VII50_11695"
                     /inference="COORDINATES: protein motif:HMM:NF024921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_function: GO:0030973 - molybdate ion binding [Evidence
                     IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HEY5051557.1"
                     /translation="KAASSTSSAATPSAATPSVSGTVVVFAASSLKATFTTLATNFEQ
                     ANPGVKIVPSYGGSDTLAAQITQGAPVDVFAAASTTTMGTVATAGDGVGTPQVFAKNE
                     LEIAVSPSNPDHIASLADVAKSGVKLAVCAATVPCGAAATKAFAAAKLTPHPVTLEQD
                     VASVLTKVELGEVDAGIVYQTDVKSAGSKVLGVNFAEAASAINTYPIVVVKTGKNSIA
                     GQEFVNYVVSPAGQQILQAAGFQQP"
     gene            760..1581
                     /locus_tag="VII50_11700"
     CDS             760..1581
                     /locus_tag="VII50_11700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY5051558.1"
                     /translation="MTDAATNTHRRGADPRARVPYALFVPAAIGVLFLILPIAGLLIR
                     TPWHQLPSLVSSPDVRTALRLSLETATAATMIAIVLGVPIAWVLARLDFRGRSLLRAL
                     ITLPLVLPPVAGGIALLLAYGRRGVVGSEILKAFGVSLPFTTAAVVVAETFVAMPFLI
                     VSVEGSLRGVDVRYDHAAATLGASRLFTFVHVTLPMLGPGIAAGAVLCWARALGEFGA
                     TITFAGSYPGTTQTMPLQVYLALQDNPDAAIALSLVLLVVSVAILAGLRDRWLRA"
     gene            1581..2678
                     /locus_tag="VII50_11705"
     CDS             1581..2678
                     /locus_tag="VII50_11705"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY5051559.1"
                     /translation="MPAGATAAAAPASLDAHIVVERQGWRLDAKLHVPGGTVLALLGP
                     NGAGKSTALRVIAGLLPATAGHVRVGERTWEDRAAGVRLATEQRSIGVVFQDYLLFPT
                     MTARDNVAFGLRAKGLEKAAAREVANGWLARVGLVEEAGQRPAQLSGGQAQRVALARA
                     LATTPDVLLLDEPLAALDAGTRMSVRGDLRRHLGEFGGATVLVTHDPLDALILADQVA
                     VIEDGAVVQTGTPHEITRNPRTRYVAQLVGLNLFAGRADGTQVRLPDGAEFVTGETHS
                     GEVFVVARPSSISVHRDLPHGSPRNVWAGTVATVEQHGDLVRLFVTGPPDIVVDVTTA
                     AMAELRLGVAERVWLSVKATDLTVYDVGAAG"
     gene            complement(2675..3820)
                     /locus_tag="VII50_11710"
     CDS             complement(2675..3820)
                     /locus_tag="VII50_11710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HoxN/HupN/NixA family nickel/cobalt transporter"
                     /protein_id="HEY5051560.1"
                     /translation="MGTQTQSRPGGIASRIGPRIRQGLSRAEWTKLAGMFAVIIALNV
                     IGWFIIIALVAPHHYTVGTKTFGIGIGLTAYTLGMRHAFDADHIAAIDNTTRKLMGDG
                     KRPLSVGFWFSLGHSSVVFLLAFLLSLGVKALVGPVKNDTSALHHYTGLIGTSVSGTF
                     LFVIAAINVIILVGIMRVFRDMRRGVYDEETLEHHLANRGLMNRILRPIMKSITKPGQ
                     MYGVGLLFGLGFDTATEVSLLVLAAAGAASTLPWYAILALPILFAAGMCLFDTIDGTF
                     MNFAYGWAFSRPVRKVFYNITITSLSIMIAAVIGGVELLGLVSQQLNVTGRFWTDMQN
                     ANINTLGFVIVGLFVVTWAVALLVWRFGHIEEKWTAGMRQRALRDAD"
     gene            4067..4543
                     /locus_tag="VII50_11715"
     CDS             4067..4543
                     /locus_tag="VII50_11715"
                     /inference="COORDINATES: protein motif:HMM:NF013629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HEY5051561.1"
                     /translation="MAAAKTPPKTQTHGAERLSAAVGELRAQGTRITDARVAILEALS
                     ATDAHLSADDIAHAVNGLQPRVHRATVYRTLESLVTAGLVAHTHLGHGNAVYHLTTVP
                     HAHCQCQRCETIIDVPRTLLRGVSSRLLDDYGFNLDTGHSALLGLCKRCAEVGETR"
     gene            complement(4486..6543)
                     /locus_tag="VII50_11720"
     CDS             complement(4486..6543)
                     /locus_tag="VII50_11720"
                     /inference="COORDINATES: protein motif:HMM:NF013713.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5051562.1"
                     /translation="MTIRWRLFLASVLMLFLELSLIRWLGANVVHLSYFSNFVLLGSF
                     LGIGLGFLRAAGPAHADRPLPFYSPVALLGLLGFVSAYPVTVDRSSSQLIFFTSLQAS
                     GPPIWLTLPCIFLAVAAVLAGPGEIVGACFRQLPRLEAYRFDLLGSLAGIATFTGLSF
                     LDAPPLLWFGIACVLLIALMGRPAVSVTVTLAVALLLLLVYPLRHDTGVFWSPYYKVS
                     TTAVDDGKGGTAWNVDVNGVPHQRLTSAATRAAEEPWYLEPYKRIPTVPKNVLIVGAG
                     TGTDVAIALAQGAQHVDAVEIDPTLLRFGRQHDPDHAYQDPRVSAHVNDGRAFLEQTK
                     TKYDLILFALPDSLTLVSGASSLRLESYLFTKQAVEVARSHLAPGGAFSMYNFYREQW
                     LVDRLAGTVDDVFGHDPCIYDQQAAESLAVIVAGLTPADQVCAQTWVRPASTPAPATD
                     NKPFLYLKDSTIPSLYLAALGLIIAGSAIAIGGVLLANAAAGGKGVRRRFSCQLAGMW
                     GYRDLFLLGAAFLLMETRSVTSFALLFGTTWLVNALVFAGVLAAVLAAVEVTRRFPTP
                     PIPAMYALLIGGLALNWLVPSSWLLSLAFLVRIVVAVVIAFLPIFAANIVFAKRFSET
                     GDAALSFGTNLLGAMLGGCLEYLSLVFGYHALLILAALLYVGAYLVSPTSAHRLQRPS
                     NAL"
     gene            6988..8055
                     /locus_tag="VII50_11725"
     CDS             6988..8055
                     /locus_tag="VII50_11725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020661539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEY5051563.1"
                     /translation="MTDVAQLAGVSHQTVSRVINKHPSVTATTRTRVLAAIEELGYRR
                     NSAARTLVTGKSQTLGVVTLNSTLYGPASTLYGIEQAALESQYFVTVASLRSTDRPAV
                     RQAIGRLVDQGVEGVVVIAPLDSLAQALASLPAGLPVVAVEGDAAADIDVVTVDQHAG
                     AQAATQHLLDLGHATVWHVSGPSDWLEAQHRTTGWRATLERAGVEVPPPLRGDWSARS
                     GFETGRVLAGMPDVTAVFVANDSMAVGVLRAFHERGRDVPGDVSVVGFDDVPEAAYFS
                     PPLTTIRQDFGEVGRRSLALLVDQIESGARAAERHVVEATLIVRDSTAPAGTASRAST
                     ASPATSRRNVQQKPPTKARPH"
     gene            8094..9761
                     /gene="araB"
                     /locus_tag="VII50_11730"
     CDS             8094..9761
                     /gene="araB"
                     /locus_tag="VII50_11730"
                     /EC_number="2.7.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008741 - ribulokinase activity [Evidence
                     IEA];
                     GO_process: GO:0019568 - arabinose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulokinase"
                     /protein_id="HEY5051564.1"
                     /translation="MSDNTDQYVIGVDYGTLSGRALVVRVSDGQEMGSAVHSYAHGVM
                     DRTLPATGATLPPNWALQDPEDYIDVLRDAVPKALSASGVAPEQVIGIATDFTACTVL
                     PVLADGTPLCRDDRFRNRPHAYVKLWKHHAAQSHAERINGTAAARGESWLPRYGGRIS
                     SEWEFAKGLQLLEEDPEIYAATQRFIEAADWIVWQLCGKETRNVCTAGYKGIYQDGGW
                     PSRDFLAALNPGFADFAHDKLQFPLSPLGGRAGGLTAQAAAWTGLPEGIAVAVGNVDA
                     HVTAPAAKATLPGQMLAVMGTSTCHVMNADRLAEVPGMCGVVENGIVQGLWGYEAGQS
                     GVGDIFAWFVDNSLPAEYLTRAESLGMDAHEYLSSLSARQRVGAHGLVALDWESGNRS
                     VLVDHSLSGVIVGLTLSTKPEDIYRALVEATAFGTRKIIETFEEAGVPCEEFVVAGGL
                     LKNAFVMQVYADVTGKAMSLLDSEHGPALGSAIHAAVAAGAYADVTTASASMGRALHD
                     AYLPDPGNHQIYNRLYAEYSALHDYFGRGTNDVLHRLHDLRIEATAP"
     gene            9758..>10234
                     /locus_tag="VII50_11735"
     CDS             9758..>10234
                     /locus_tag="VII50_11735"
                     /inference="COORDINATES: protein motif:HMM:NF012804.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HEY5051565.1"
                     /translation="MTAPPHSVGAIRTSVAQLHAELLRWGLVVWTAGNVSARVPGEDL
                     FVIKPSGISYDELTPQIMIVCDLDGQVVDGDHAPSSDTAAHAYVYRAMPDVGGIVHTH
                     STWATAWAQARREIPVLGTTHADLTAHPIPLTRELSAEEISADYERATGGAVVEALA"
BASE COUNT         1812 a         3443 c         3365 g         1614 t
ORIGIN      
        1 agaaggctgc gtcatcgacg tcctcggcgg ctacgcccag cgcggcgacg cccagcgtgt
       61 ccggcacggt cgtcgtgttc gcggcatcgt cgctgaaggc cacgttcacg acgctggcca
      121 cgaacttcga acaggcaaac ccaggcgtga agatcgtgcc gtcctacgga ggttccgaca
      181 cgctcgcggc ccagatcacc cagggcgcgc cggtcgacgt ctttgccgcc gccagtacca
      241 cgacgatggg caccgtcgcc acggccggtg atggcgtcgg gacgccgcag gtgttcgcca
      301 agaacgagct tgagatcgcg gtgtcgccga gtaaccccga ccacatcgcg agcctcgcgg
      361 acgtggcgaa gtccggggtg aagttggcgg tgtgcgcggc cactgtgccg tgcggcgcgg
      421 cggcgaccaa ggcctttgcg gcagccaagc tgactcccca cccggtgacg ctcgaacaag
      481 acgttgcctc ggtcctgacc aaggtcgaac tcggcgaggt cgatgcgggc atcgtgtacc
      541 agacggatgt gaagtcggcg ggcagcaaag tgctcggcgt gaacttcgcc gaagcggcaa
      601 gcgcgatcaa cacctacccg atcgtggtcg tcaagaccgg gaagaactcg atcgccgggc
      661 aagagttcgt gaactacgtc gtgtcgccgg ccggacagca gatcctgcag gcggccgggt
      721 tccagcagcc ctgaccccac gcgggccaca cttgcgcaca tgactgacgc cgccactaac
      781 acacatcgcc gcggcgccga ccctcgggcg cgggtacctt atgcgctgtt cgtacccgcg
      841 gcgatcggcg tcctgttcct gatcctgccg atcgcggggc tcttgattcg cacgccctgg
      901 caccaactgc cctcgttggt gagcagcccg gacgtccgca ctgccttgcg tttgtcactg
      961 gagactgcga ccgcggcgac gatgatcgcg atcgtgcttg gcgtgccaat tgcgtgggtg
     1021 ttggcccggc tggattttcg cggccgctcg ctgctgcgag cattgatcac cctgcctctc
     1081 gtactgccac ccgtcgccgg cggtatcgca ctgctcctgg catacggccg ccgcggcgtc
     1141 gtgggcagcg aaatcctcaa ggcgttcggg gtatcgctgc ccttcacgac cgcggccgtg
     1201 gtcgtggccg aaacgttcgt cgcgatgccg ttcctcatcg tgtcggtcga aggctccctg
     1261 cgcggggtcg atgttcgcta cgaccacgca gctgcgacgc tcggggcctc gcgactcttc
     1321 accttcgtgc acgtgacgtt gccgatgctg ggcccaggca tcgccgccgg cgccgtgctg
     1381 tgctgggccc gcgcactcgg ggagttcgga gcaacaatca cgttcgccgg cagctatccc
     1441 gggacgacgc agaccatgcc actgcaggtc tatctcgccc tccaagacaa tccggacgcc
     1501 gcgatcgcgc tgagcctcgt cctgctcgtc gtgtcggtgg cgatcctggc cggactgcgt
     1561 gaccgctggc tgcgcgcgtg atgccagcag gcgcaactgc ggccgccgcg ccagcatcgc
     1621 ttgacgcaca catcgtcgtc gagcggcagg ggtggcggct cgatgcgaaa cttcacgtcc
     1681 ccggtggaac agtgctcgca ctccttggtc cgaacggggc cggcaagtcg accgcgttgc
     1741 gggtcatcgc cggactgctg ccggcaacgg ctggtcatgt gcgagtcggc gagcgcacgt
     1801 gggaagaccg cgcagccggc gtccggctcg cgaccgagca acgctcgatc ggcgtcgtgt
     1861 tccaggacta cctgttgttt ccgacgatga ccgctcgcga caacgtggcg ttcggcctgc
     1921 gcgcgaaagg cctggagaag gcggccgcgc gcgaggtagc gaacgggtgg ctggctcgcg
     1981 tcggactggt cgaggaggcc ggccagcggc ctgcgcagtt gtccggcggg caggcccagc
     2041 gagtggcatt ggccagggcg cttgcgacga cgccggacgt cttgctactc gacgaaccgc
     2101 tggccgcgct cgacgccggc acccgcatga gcgtgcgcgg cgatctgcgc cggcaccttg
     2161 gcgagttcgg cggcgcgacc gtcttggtca cccacgatcc gctcgacgcg ttgatcctgg
     2221 ccgaccaggt agctgtcatc gaagacggcg cggtcgtgca gaccggaacc ccgcacgaga
     2281 tcactcgcaa ccccagaacg cgctacgtcg cccagctcgt tggtctcaac ctcttcgcgg
     2341 gccgggccga cggcacgcag gtcaggttgc ccgacggtgc cgagttcgtc acaggggaga
     2401 cacactccgg ggaggtgttc gtcgtcgcgc gcccgtcgag catttcggta caccgcgacc
     2461 tcccgcatgg cagcccgcgc aacgtgtggg cgggaaccgt cgcgacggtc gagcaacacg
     2521 gcgacctggt caggctcttc gtcaccggcc cacccgacat cgtcgtcgac gtgacgacag
     2581 cagcgatggc tgaacttcgg ctcggcgtcg ccgaacgcgt gtggctctcg gtgaaagcca
     2641 cggacctcac cgtctacgac gtaggcgccg cgggttagtc cgcgtcgcga agcgcgcgct
     2701 gtcgcatacc ggcagtccac ttctcttcga tgtgtccgaa ccgccagacc agcaatgcga
     2761 ccgcccaggt caccacgaag agcccgacga tgacgaaccc gagcgtgttg atgttcgcgt
     2821 tctgcatgtc ggtccagaaa cggccggtga cgttcagctg ctgcgatacc aggccgagca
     2881 gttcgacccc accaatgacc gccgcaatca tgatcgacag gctggtgatg gtgatgttgt
     2941 aaaagacctt gcgcaccggt ctggagaacg cccagccgta ggcgaagttc atgaacgtgc
     3001 cgtcgatggt gtcgaacaag cacatgccgg ccgcgaaaag gatcggtagc gcgaggatgg
     3061 cgtaccacgg cagcgtactc gccgcccccg cggccgcaag cacaagaagc gacacctcgg
     3121 ttgcggtgtc gaagccgaga ccgaagagca ggccgacgcc atacatctga cccggtttgg
     3181 tgatggactt catgatcggt cgcagtatcc ggttcatcag gccccggttg gccagatggt
     3241 gctcgagcgt ctcctcgtcg tagacgccgc gccgcatgtc acgaaacacc ctcatgatgc
     3301 caacaaggat gatcacgttg atcgcggcaa tgacaaagag aaacgtcccg gagaccgatg
     3361 tgccgatgag tccggtgtaa tggtgcaggg cggaggtgtc gttcttcacc gggccgacga
     3421 gtgccttcac gccgagcgac agcaagaacg ccagcaggaa gacgaccgac gagtgcccga
     3481 gggagaacca gaacccgacc gacagcggtc gcttgccgtc gcccatcagt tttcgggtgg
     3541 tgttgtcgat cgccgcgatg tggtcggcgt cgaaggcgtg cctcataccg agggtgtaag
     3601 ccgtcagtcc gatgccgatc ccaaaggtct tggtgcccac ggtgtagtgg tgcggcgcga
     3661 ccagcgcgat gatgatgaac cagccgatga cgttcaacgc gatgatgacg gcgaacatcc
     3721 cggcgagctt ggtccactcg gcccggctga gaccctgtct aatgcgaggc ccgatccgtg
     3781 acgcaatgcc acccggtcga ctctgcgtct gggtacccat cgagctacct ccgttgactt
     3841 ccgtcggggc tggctgatcc ccgacgccgc gtggcgccat cagatgcgac aggttcgcag
     3901 ccgggcgggg tttggattgg tccccgccgc ccgcgtcggc tcacctgcag accccgccac
     3961 cgtaacatcc cagttgcgac tgtgtcgcat atcttttgcc tggccccagc gcacccccgg
     4021 cgcggctacg ctgctaccgc gcgcattcga aggagctgac ccggacgtgg ctgcggcaaa
     4081 gaccccgccc aagacccaga ctcatggcgc cgagcggctg tctgcagctg tcggtgagct
     4141 ccgcgcgcag ggcacccgaa tcaccgatgc cagagtggcg atcctcgagg ccctatcggc
     4201 caccgacgcg cacctgtccg ccgacgacat cgcgcacgca gtgaacgggc tacagccgcg
     4261 cgtgcaccgc gcgaccgtgt acaggacgct ggaatcgctc gtcaccgcgg gcctggtcgc
     4321 ccacacccac ctcggccatg gcaatgcggt ctatcacctg accacggtcc cgcacgccca
     4381 ctgccagtgt cagcgctgcg agacgatcat cgacgtcccg cgcaccctgc ttcgaggcgt
     4441 cagcagccga ctcctcgacg attacggctt caatctcgac accggtcaca gcgcgttgct
     4501 cggcctttgc aagcggtgcg ccgaagtcgg cgaaacgaga taggcaccca cgtatagcaa
     4561 cgccgccaag atcagcagcg cgtggtaccc gaagacgaga gacagatact ccaggcaccc
     4621 gccgagcatc gcgccgagca ggttggtgcc aaacgacagc gccgcatcgc ccgtctccga
     4681 aaaacgtttc gcgaagacga tattcgccgc gaatatcggc aggaacgcga tcactaccgc
     4741 gacgacgatc cgcaccagga acgccagcga gagcagccac gacgacggca ccagccagtt
     4801 cagcgccaag ccaccgatca gcaacgcgta catcgccgga atcggtggcg tcgggaaacg
     4861 ccgcgtcact tcgaccgccg caaggaccgc cgccaaaaca ccggcgaaaa cgagcgcgtt
     4921 caccagccac gtcgttccga acagcagcgc gaagctcgtg acactgcgtg tctccatcag
     4981 caggaaagcc gcgcccagca ggaacaggtc gcggtagccc cacatcccgg cgagctgaca
     5041 gctaaagcgc cgacgcactc ctttcccgcc ggccgccgcg ttcgccaaca acacaccgcc
     5101 gatggcgatc gcgctgccgg cgatgatcag accgagcgcg gccaggtaca gcgaggggat
     5161 cgtgctgtcc ttcaggtaaa ggaagggctt gttgtccgtc gcaggagccg gcgtcgacgc
     5221 cggccgaacc cacgtctgcg cgcacacctg gtctgccggc gtcaggccgg ccacgatcac
     5281 cgcgaggctc tcggcggcct gctggtcgta gatgcacggg tcatggccga agacgtcgtc
     5341 gacggtgccg gccagccggt cgacgagcca ttgctcgcgg tagaagttgt acatggagaa
     5401 cgcgcccccc ggcgccaggt gcgagcgggc gacctcgacg gcctgcttgg tgaagaggta
     5461 gctctccagg cgcagcgaac tggctcccga cacgagcgtg agcgagtcgg gcagcgcgaa
     5521 caggatcagg tcgtacttcg tcttggtctg ctcaaggaac gcgcggccgt cgttgacgtg
     5581 tgcgctgacc cgcgggtctt gataggcgtg atccgggtcg tgctggcgac cgaagcgaag
     5641 cagtgtcgga tcgatctcga ccgcatcgac gtgctgcgcg ccctgcgcca gcgcgatcgc
     5701 gacgtcggta ccggtgccgg cgcccacgat gaggacgttc ttcggaaccg tcgggatccg
     5761 cttgtatggc tcgagatacc agggttcctc tgctgccctt gtggcggcgg acgtcagtcg
     5821 ctggtgcgga actccgttta cgtcgacgtt ccacgcggtg ccgcctttgc cgtcgtcgac
     5881 cgcggtcgtc gacaccttgt agtagggcga ccagaacacg ccggtgtcgt gccgcaacgg
     5941 atacaccagg agcagcaaca gtgccaccgc caacgtgacc gtcaccgata ccgcgggcct
     6001 gcccatcagt gcgatcaaga gtacgcatgc gatgccgaac cacagcaggg gcggcgcgtc
     6061 gaggaacgac aacccggtga aggtggcgat gccggccaga ctgccgagca gatcgaatcg
     6121 gtacgcctcg aggcgaggca gttgccgaaa gcaggcaccg acgatctcgc caggacctgc
     6181 gagcactgcg gcgaccgcga ggaagatgca gggcagcgtc agccagatgg gcggaccgct
     6241 tgcctgcagg ctggtgaaga agatcagctg gctgctacta cgatccaccg tgaccggata
     6301 ggcgctgaca aagcccagca gcccaagtag cgcgacgggt gagtagaacg gcaatggccg
     6361 gtcagcatgc gccgggccgg ccgctcgcag gaaccccagt ccgatcccga ggaaggatcc
     6421 gaggagcacg aagttggaga aatacgagag gtggacgacg ttcgcgccga gccagcggat
     6481 gagcgagagc tccaggaaca gcatgagcac gctggcgaga aatagtcgcc atcggatcgt
     6541 cactggcgaa cctctcgtcg tccggtttgc cgtcgtctgc gccactggca tgcctgggtg
     6601 tggtgccgta cctgggggta gtgccgtgcc tggcagcagg cggctgggtt cgcagcgcct
     6661 gcgtcgtatt cagccgcatt ccggcgccgg tggcaatgcc cactcccagg cccgcgcacc
     6721 cgcccactcc cctttccagc ctcggctccg gcgggtcgac ccctatgacg tgccgagagg
     6781 cggtattggt tgcccgtgat cccgctgaca cggaaagagg taacggttga gcgacgttgc
     6841 attgacaggg tgcggttggt cgacatactg agacggctat gttaacgctc actcaggccg
     6901 aaaccaccca ggcggccgca cgtggctagc ccgcctgccg ggccgcctag tcggacaacc
     6961 tcaggcggcg gtttgcgtgc gcccgccatg accgacgtcg cgcaactagc tggagtatcg
     7021 catcagaccg tgtcgcgggt catcaacaag caccctagtg tcacggcgac gacccgcacg
     7081 cgcgtgttgg ccgccatcga ggaattgggc tatcgccgca acagcgcagc ccgcacgctg
     7141 gtcaccggaa aatcgcagac cctgggcgtg gtcaccctca acagcacctt gtacggcccg
     7201 gcatcgacgc tgtacggcat cgaacaggcg gcgctggagt cgcaatactt cgtcactgtt
     7261 gcgagcttgc gctcgaccga tcgacccgca gtccgccagg ccatcggccg cttggtggac
     7321 cagggagtcg aaggggtcgt tgtcatcgca cccctcgact ccctggctca ggcactcgcg
     7381 agcctgccgg ctggcttgcc agtagtggcg gtcgagggcg acgcggccgc cgacatcgac
     7441 gtcgtaacgg tcgaccagca cgcaggcgcc caggcggcca cccaacacct gctggacctc
     7501 ggccacgcga cggtctggca cgtttctgga ccgagcgact ggctcgaagc tcagcaccgc
     7561 accaccggct ggcgagccac cctcgagcgc gccggcgttg aagtgccgcc gcccctacgc
     7621 ggcgactgga gcgcgcggtc ggggttcgag accggtcgcg tccttgccgg catgcccgac
     7681 gtcaccgcag tctttgtcgc gaatgactcg atggcggtcg gagtcctgcg cgcgtttcac
     7741 gagcgcggcc gcgatgtgcc cggtgacgtg agcgtcgtgg gattcgacga cgtgccggag
     7801 gcggcctact tcagcccgcc gctgaccacg atccggcagg acttcggcga ggtcggtcgc
     7861 cgcagtctcg cgctgttggt cgaccagatc gaatccggtg cccgcgcggc ggaacgccac
     7921 gtcgtcgaag cgacgctcat cgttcgcgac agcaccgccc cggcgggcac agcgagcagg
     7981 gccagcacgg ccagcccagc aacctccagg cgcaacgtcc agcagaaacc acccacgaaa
     8041 gcgcggccgc actgatgtcc aacgaaccct cagccgatgc gtcccacgag tatgtgagcg
     8101 ataacacaga ccagtacgtg atcggtgtcg actacggaac gctgtccggc cgggcgctgg
     8161 tggtgcgggt gtccgacggg caggagatgg gcagcgccgt ccattcgtac gcgcacggtg
     8221 tcatggaccg cacactcccc gcgaccggcg ccacgctgcc gccgaactgg gcgttgcaag
     8281 accccgagga ctacatcgac gtcctgcgcg acgcagttcc gaaggcgctg tccgccagcg
     8341 gcgtcgcgcc ggagcaagtg atcggcatcg cgaccgactt caccgcgtgc acggtactgc
     8401 cggtgctcgc cgatggcacc ccgctgtgcc gcgacgatcg cttccgcaat cgcccgcacg
     8461 cgtacgtgaa gctgtggaag caccacgcag cacagtcgca tgccgagcgc atcaacggca
     8521 ccgccgcagc ccgtggcgag agctggctgc cgcgctacgg cggccgcatc tcctcggagt
     8581 gggaattcgc caaaggactg cagttgctcg aggaagaccc agagatctat gccgcgacgc
     8641 aacgcttcat cgaagcagcg gactggatcg tctggcagct gtgcggcaag gagacacgca
     8701 acgtctgcac ggccgggtac aagggcatct atcaggacgg cggttggccg agccgtgact
     8761 tcctcgccgc gttgaatccc ggcttcgccg acttcgcgca cgacaagctg cagtttccgc
     8821 tctccccact gggcggccgg gcgggcggcc tgactgcgca ggcggccgcc tggaccggcc
     8881 ttcccgaagg catcgcggta gccgtcggga acgtcgatgc gcacgtcacg gcgcccgcgg
     8941 cgaaagcgac cttgccgggt cagatgcttg cggtgatggg tacctcgaca tgtcacgtga
     9001 tgaacgctga tcggctcgcc gaggtaccag gaatgtgtgg tgtcgtcgaa aacggaatcg
     9061 tgcaaggtct gtggggatac gaagccggtc agagcggggt tggtgacatc tttgcctggt
     9121 tcgttgataa ctcactgcct gccgagtacc tcacgcgtgc cgagtcactc gggatggacg
     9181 cgcacgagta cctcagctcg ctgagtgctc ggcagcgggt gggcgcgcac ggtctcgtcg
     9241 cactggactg ggagagtggc aaccgatcgg tgctcgtcga ccactcgctg tctggtgtca
     9301 tcgtcggtct caccctgtcg accaaacccg aggacatcta ccgggcactc gtcgaagcca
     9361 ccgcgttcgg tacccgcaag atcatcgaga cgttcgagga ggccggcgta ccgtgcgagg
     9421 agttcgtggt cgccggcggc ttgctgaaga atgcattcgt gatgcaggtg tacgccgacg
     9481 tgaccggcaa ggcgatgtcg ctgctcgact ccgagcatgg gccagccctc ggctcggcga
     9541 tccacgccgc ggtcgcagcc ggcgcgtatg cggacgtcac gacagcgtcg gcatcgatgg
     9601 gccgcgcgct ccacgacgcc tacctgcccg acccggggaa ccaccagatt tacaaccgcc
     9661 tctatgcgga gtactcggcg ttgcacgact acttcggccg cggcaccaac gacgtgctgc
     9721 acaggttgca cgacctgcgc attgaagcga ccgcaccgtg acagcaccgc cgcactccgt
     9781 aggcgcgatc cgtacatcgg tcgcccagct gcacgcggaa ctgttgcgct ggggtctcgt
     9841 cgtctggacc gccggcaacg tctcggctcg ggttcctggc gaggatctgt tcgtcatcaa
     9901 gccaagtggg atcagctacg acgagctcac gccgcagatc atgatcgtgt gcgacctcga
     9961 cggccaggtg gtcgacggtg accacgcgcc gtcaagcgat acggcggcgc acgcgtacgt
    10021 ctatcgcgcg atgcccgacg tcggcggcat cgtccacacc cactcgacat gggcaacggc
    10081 gtgggcgcag gcgcgacgag agatccccgt gctcgggacc actcacgccg acctgacggc
    10141 gcacccgatc ccgctgacgc gcgagttgag cgccgaggag atctctgctg actacgagcg
    10201 cgcgaccggt ggcgccgtcg tcgaggcgct cgcc
//
LOCUS       DATCBJ010000584         4009 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_531701, whole genome shotgun sequence.
ACCESSION   DATCBJ010000584 DATCBJ010000000
VERSION     DATCBJ010000584.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 4009)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4009)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4009
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_531701"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..653)
                     /locus_tag="VII50_11740"
     CDS             complement(<1..653)
                     /locus_tag="VII50_11740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY5051566.1"
                     /translation="MVDVQATARAPRSGGAIVAEGLVKHYGKVRALDGIDLRVEEGTV
                     LGLLGPNGAGKTTAVRVFTTLLKPDAGTATVAGLDVVRDADAVRRTIGVSGQYAAVDE
                     NLTGRENLDMVGRLYHLGKSVSLARADELLERFDLVEAANRVVKTYSGGMRRRLDLAG
                     SLVAQPRVLFLDEPTTGLDPRGRLGMWDVIEELVDGGTTLLLTTQYLEEADRLAQSIV
                     "
     gene            complement(806..1483)
                     /locus_tag="VII50_11745"
     CDS             complement(806..1483)
                     /locus_tag="VII50_11745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5051567.1"
                     /translation="MAAPHFAAAQSITAAQPFAAAPRLAPDPGDLFVAQPDLHLVSQP
                     DVSQPDLRVVVTAPSWSVPPAASVSVLAALTEGDKARPDFAPSNSGDAFPTRTRALSL
                     LLGQVLAVFGIFTLSLAATSIGRHVSHVGSISVWLLATSCVFVGVAFAVVVAARVQHD
                     IMGGGWYGGFSPRYISPQVALYVISPSGISAATRRLNSSPARVIFVEYGLLAAAVLIV
                     LHRLVGR"
     gene            complement(1946..3178)
                     /gene="ilvA"
                     /locus_tag="VII50_11750"
     CDS             complement(1946..3178)
                     /gene="ilvA"
                     /locus_tag="VII50_11750"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004794 - L-threonine ammonia-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0006549 - isoleucine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase"
                     /protein_id="HEY5051568.1"
                     /translation="MTGAVVPRDPQEWVADARAAATLLGGVARRTPIESSRALAELVG
                     GDVRLKCENLQRAGSFKIRGAYVRIARLTDAERARGIVAASAGNHAQGVALASSLLGA
                     KSTVFMPEGAPLPKVEATRKYGADVRFFGHTLDEALAEAISFAAATGAVLIHPFDHPD
                     IVAGQATVGLEIVEQCPDARTIVVSTGGGGLTAGLAVVVKALRPDITVVGVQAQGAAA
                     FPASLEAGTPTRLASMSTIADGIAVGCPGELTFELVRDYVDAVLTVSEDSLSRALLLC
                     LERAKLVVEPAGVAAVAAIIDAPQSFEPPVVAVLSGGNVDPVLLMGVLRHGLAAAGRY
                     LSFQVTISDRPGALAALLHALAAAGGNVLDVVHQRTAPTLRLDEVAVALQVETRGPTH
                     SDEVVEMLRSRGYRLEFT"
     gene            3265..>4009
                     /locus_tag="VII50_11755"
     CDS             3265..>4009
                     /locus_tag="VII50_11755"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY5051569.1"
                     /translation="MRTQLAPGTTDHVVEGIRIRVKTYGGPGDGLDNSPAVLLLHGFP
                     TSSQLWRQVGPEMCAGTDDDLFVLAPDLVQLGESERTGSRIDLATQARLLLGMLDACG
                     VDKVIVAGHGLGGAVAVHIAALDASRVAGLALLDSPLHADAWPVAGALPMLIPGVRAI
                     CGRALPVVPWLAERVLATLVGSGSDATSVRPLDRYVEELRKPGAARGLVHFATAVDPV
                     AIEAAWHIVRASPPPTLVMWGADDRVHNIT"
BASE COUNT          739 a         1345 c         1348 g          577 t
ORIGIN      
        1 acgatcgatt gagcgagccg atcggcttcc tccagatact gcgtggtcag caacaacgtc
       61 gtcccgccat cgaccagctc ttcgatgacg tcccacatcc ccaggcgacc gcgcgggtcg
      121 agacccgttg tcggctcgtc gaggaacagc acccgaggct gcgcgaccag gctgccggcg
      181 aggtcgaggc ggcggcgcat gccaccggag tacgtcttca cgacccgatt cgcggcctca
      241 acgagatcga agcgctcgag gagctcgtcg gctcgcgcga gagacaccga cttaccgagg
      301 tgatagaggc ggccgaccat gtcgaggttc tcgcgaccgg tcaggttctc gtcaaccgcc
      361 gcatactggc ccgacactcc aatggtccgg cgaaccgcat cggcatcgcg aacgacatcg
      421 aggccggcca cggtggcagt tccggcgtcc ggcttgagaa gggtggtgaa gacccgcacc
      481 gcggtcgtct taccggcgcc gttgggcccg agtaggccga gcacggtgcc ctcttcgacg
      541 cgcaagtcaa tgccgtcgag cgcgcgtacc ttgccgtagt gcttgacgag gccctcggcc
      601 acgatcgcgc cgcccgatcg cggagcgcgc gcagttgctt gaacatcaac catgtaagga
      661 gagactccct cgagtcgccg aaatcatcgg ttcgttcaaa ccggcagcag gaactgcgtt
      721 ccggaacaag cacgcatgtg tgaaccaggc aacacagcag gctatcgagc ggccctgcgg
      781 aagcgtcaaa ttgcttacat caacctcagc gaccaaccaa gcggtggagc acgataagaa
      841 cagctgccgc cagaaggccg tactcgacaa agatcacgcg tgcaggcgaa gagttcagtc
      901 gacgggtcgc ggccgagatc cccgatgggc tgatcacgta gagcgcgact tgcgggctga
      961 tgtatcgggg cgagaaccct ccgtaccagc cgccccccat gatgtcgtgc tgaacgcgcg
     1021 cggctacgac gacggcgaac gcaacgccca cgaacacgca cgaggtggcg agcaaccaaa
     1081 cggagatgct gcccacgtgc gacacgtgac ggccgatgga cgtggctgca agcgaaagcg
     1141 taaagatgcc aaacactgcc agcacctgcc ccaggagcag ggatagcgca cgagttctgg
     1201 tcggaaacgc gtcgccggag ttcgacggcg cgaagtccgg tcgcgctttg tccccctccg
     1261 tcaacgccgc aagcaccgag actgacgcag ccgggggaac ggaccacgat ggcgcggtaa
     1321 cgacgacacg gagatcgggc tgcgaaacat cgggctgcga aacgaggtgt aggtcgggct
     1381 gagccacaaa gaggtcgccc gggtcgggcg ccaaccgcgg ggcggctgca aagggctgcg
     1441 cggccgtaat ggactgcgcg gctgcaaagt ggggggcggc cacaaagggc tgcgcggccg
     1501 cgatcgactg ggcggctgca aagtgaggag cggctgcaaa gcgaggagcg gctgcaaagg
     1561 gctgcgcgac cgtaatggac tgagcggccg caaagtggcc gcccggattt ggtgcaaagc
     1621 gcgaaggggc tgcaaagcgc ggatccggcg ccgctacacg gcgggaagcg acccgaggtc
     1681 cgcccgagtg ccggctgcgg gcgccgccat tgttgccgcc ccggctacgc acaaccaagg
     1741 ccgtgccgag caagaccagc aatgcggccg acaagatggg cacgaggggc aatggcgtca
     1801 cgtgcgacaa agcaaggtgc acggcatccg actttcgggg ggggacggct gaccaagcac
     1861 gcagaaatcg agcgctcagt tagttgtttc gaccccgccg gagggcgacc ttgagctttc
     1921 gcttgcgtca cccgggtgaa cttcgctagg tgaactcgag gcggtagccg cgtgaccgga
     1981 gcatctctac cacctcgtcg gagtgcgtcg gccccctggt ttcgacctgc agcgccaccg
     2041 cgacctcgtc gaggcgcagc gtcggcgcag tgcgctggtg caccacatcg agaacattgc
     2101 cgcccgcagc ggccagcgcg tgcaacaacg ccgcgagcgc gccaggccgg tcgctgatcg
     2161 tcacctggaa cgacagatag cggcccgccg ccgcgagtcc gtgccgcaag acgcccatca
     2221 gcagcaccgg atcaacgttg ccgcccgaca acaccgcaac aacgggcggc tcgaacgact
     2281 gcggggcatc gatgattgcg gcgactgcgg cgaccccggc cggttcgacg accagcttgg
     2341 cgcgctcgag acagagcaac agcgcgcgcg acagcgagtc ctcggagacc gtcaacaccg
     2401 cgtcgacata gtcgcgaacc agctcgaacg tcaactcacc aggacacccc acggcgatgc
     2461 cgtcggcgat cgtcgacatg ctcgcgagcc tggtcggcgt cccagcctcg agcgaagccg
     2521 ggaacgcggc cgcaccttgg gcttgcaccc cgacgacagt gatgtcgggc cgcagggctt
     2581 tgacgacgac agcaagaccc gcagtgagtc cgccgccgcc ggtgctgacc acgatggtcc
     2641 gcgcgtcagg gcactgctcg acgatctcca gcccgaccgt cgcttgaccg gccacgatgt
     2701 cggggtgatc gaacgggtga atcagcaccg cgccggtggc ggccgcgaac gagattgcct
     2761 cggcgagcgc ttcgtcaagg gtgtggccga agaaccgcac gtcggcgccg tacttgcggg
     2821 ttgcctcgac cttcggcagc ggtgcacctt cgggcatgaa caccgtcgac ttggcgccga
     2881 gcagcgatga ggcgagcgcg acaccttgcg cgtggttgcc cgcgctcgcc gcgacgatgc
     2941 cgcgggcccg ctcggcgtcc gtcagtcgcg cgatgcgcac gtaggcgccg cgaatcttga
     3001 acgatccggc gcgttgcaag ttctcgcatt tcaaccggac gtcgccgccg accaattcgg
     3061 cgagcgcgcg ggaggactcg atcggcgtgc ggcgcgcgac cccaccgagg agcgtcgcgg
     3121 ctgcacgcgc gtcggcgacc cactcctgcg ggtcgcgcgg cactaccgct ccggtcatgc
     3181 ccgaagtctc gcaccgatcg ggaggaaaaa agattgcctc gcctcagccg gtttcagcgt
     3241 gccgatccgg tgcaggatga acgaatgcgc acccaactcg ctccgggcac gaccgaccat
     3301 gtcgtcgagg gcatccggat ccgggtcaag acctacggcg gaccaggtga cggcctcgac
     3361 aactcaccgg cggtcctgct gctgcacggc ttcccgactt cgtcgcagtt gtggcgccag
     3421 gtcggtcccg agatgtgcgc cggcaccgac gacgacctgt tcgtgctcgc acccgacctc
     3481 gtgcagctcg gcgagtcgga gcgaaccggt tcacgcatcg acctcgcgac gcaggcccgg
     3541 ttgttgctcg gcatgctcga tgcatgcggc gtcgataagg tgatcgtcgc cgggcacgga
     3601 ctcggcggtg ccgtcgcggt ccatatcgcc gcacttgacg cgagccgcgt tgccggcctc
     3661 gcgctgctcg actcgccatt gcatgccgac gcgtggcccg tcgccggtgc actaccgatg
     3721 ctgattcccg gcgtgcgcgc gatttgcgga cgcgcactcc ccgtcgtccc gtggcttgct
     3781 gaacgagtgc ttgcaacgtt ggtcggcagt ggcagcgatg cgaccagcgt gcggccgctc
     3841 gaccgttacg tcgaggagtt gcgcaagccc ggtgcggccc gcgggctcgt gcactttgcg
     3901 accgcggttg acccggtcgc gatcgaagcc gcgtggcaca ttgtccgcgc ctcgccaccc
     3961 ccgacgctcg tgatgtgggg cgccgacgat cgagtgcaca acattacct
//
LOCUS       DATCBJ010000585         8751 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_90053, whole genome shotgun sequence.
ACCESSION   DATCBJ010000585 DATCBJ010000000
VERSION     DATCBJ010000585.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 8751)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8751)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8751
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_90053"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            108..758
                     /locus_tag="VII50_11760"
     CDS             108..758
                     /locus_tag="VII50_11760"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF005856.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HEY5051570.1"
                     /translation="MSDQPVGQPPTAPVMSGAESEIGAVPAQSTGPAPSTSRPYGGYF
                     DEVADALAQAYPNIAQAIERSIVDRGELTLHVRRERLRELAQQLRDDQDLRFELLSSL
                     SGVHYPMDAGRELHVVYELTSMTHNRRIRLEVAAPDDDPHVPSVVAVWPTADFQEREA
                     WDMFGVIFDGHPALTRIMMPDDWPGHPQRKDYPLGGIAVEYKGATVPPPDERRQYA"
     gene            755..2086
                     /locus_tag="VII50_11765"
     CDS             755..2086
                     /locus_tag="VII50_11765"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit D"
                     /protein_id="HEY5051571.1"
                     /translation="MTTSTDPYAGVRETTEGRVYTVTGGDWDDVAADVASEHAERVVV
                     NMGPQHPSTHGVLRLILELDGETITQARLAIGYLHTGIEKNCEYRNFTQAVTFLTRAD
                     YLSPLFTESGYSMAVEKLLGITDEVPIRAQVIRVMMMELNRISSHLVWLGTGGLEVGA
                     LSAMLYAFRERELILDIFEMISGLRMNHAYVRPGGVIQDLPPGAVEKIREFVQLMPGR
                     IDEYHNLLTGQPIWIARLQGVGYLDVNACVALGVTGPLLRAAGLPWDLRKSQPYSGYE
                     TYEFDVPTQQEGDCFARYLVRMDEMRESVNIIEQCLDRLRPGPVMVEDKKIAWPAQLA
                     LGADGMGNSLDHIRHIMATSMESLIHHFKLVTEGFHVPPGQVYVPIESPRGQLGVHLV
                     SDGGTKPYRVHLREPSFVNLQAVPAVCEGSLIADVIASVASLDPVMGGCDR"
     gene            2083..2835
                     /gene="nuoE"
                     /locus_tag="VII50_11770"
     CDS             2083..2835
                     /gene="nuoE"
                     /locus_tag="VII50_11770"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HEY5051572.1"
                     /translation="MSHSHGERSGLPTVTLDPRLGPGASLADPLSDDAKARVRDLGAQ
                     VIARYPRPRSAMMPLLYLVQAEQGYVSPDGIKLVAELLGLTTAEVAAVSTFYTMYKRR
                     SCGDYHVGVCTNTLCAILGGDAIFAALKEHLGVGNAGTTPDNKVTLEHIECNAACDYA
                     PVVMVNWEFFDNQTVESSKELVDRLRAGDLPMPTRGAPLVTHVETSRVLAGFPDALVD
                     EGPSAGAESLVGLELAKERGETAPALESKDPS"
     gene            2832..4136
                     /gene="nuoF"
                     /locus_tag="VII50_11775"
     CDS             2832..4136
                     /gene="nuoF"
                     /locus_tag="VII50_11775"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HEY5051573.1"
                     /translation="MTFALTPVLTANWGTPRSWTLASYRRAGGYDSLRAALAMEPDAL
                     IAMVKDSGLRGRGGAGFPTGMKWGFIPQGDGKPHYLVVNADESEPGTCKDIPLMMASP
                     HTLLEGVLISAYAIRANQAFIYVRGEVLHVVRRLLNAVEEAKAAGFIGHNVLGSGFNV
                     DVVVHIGAGAYICGEETALLESLEGFRGQPRLRPPFPAIEGLYACPTVINNVETISSV
                     PAIVRGGAAWFKKMGTEKSPGFKIFSLSGHVARPGQYEAPLGITLRELLDATGGMREG
                     HELKFWTPGGSSTPLFTAEHLDVPLDFEGPPAHGSMLGTTALQLFDETTCVVRAVLRW
                     SEFYAHESCGKCTPCREGVFWYVQILERLERGEGDEQDIETLLDLSENILGRAFCALG
                     DGATSPVTSSIQYFRDEYIAHFDGRGCPFDPVASTVFAGASA"
     gene            4136..6646
                     /locus_tag="VII50_11780"
     CDS             4136..6646
                     /locus_tag="VII50_11780"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009072062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit G"
                     /protein_id="HEY5051574.1"
                     /translation="MTVTTNPPAGAGGGERHSAGGANQPARSDLVTLTVDGFEISVPK
                     GTLVIRAAEFLGIQVPRFCEHPLLDPVGACRQCLVEVEGQKKPMASCTTTVTEGMVVK
                     TQITSAVAEKAQNGVIELLLVNHPLDCPVCDKGGECPLQNQTMTNGYSESRFRDVKRT
                     YPKPIAISAQVLLDRERCVLCTRCTRFAQQIAGDPFIEMLERSALEQVAVYEDQPFNS
                     YFSGNTVQICPVGALTGTQYRFRARPFDLVSSPSICEHCASGCQQRNDHRRGKMLRRL
                     AGVDPQVNEEWNCDKGRWAFSYTTASDRLDTPLVRDSDGDLVPASWPEALRAAAAGLA
                     AARNGAGVGVLAGGRLTVEDAYAYAKFARVALGTNDIDFRARVHSDEELAFLGSRVAG
                     RFLDVTYDDLEAASTVLLVGLEPEEESPIIFLRLRKAALKKHAKVVSIAPFATRGLDK
                     MGGRLIATVPGGEATMLQVLASGETVEVPPGVDASGWSELLTEVAAGLALPSSVILVG
                     ERLAGVTGGLSAAAALADATGARLAWVPRRAGERGAVEAGALPNLLPGGRAVTDNDAR
                     GQVGLQWVTTIPAKPGRDTAGILEAASRGELGALVVAGVDLDDLPDPAAARAAIDAAS
                     FVVSLELRRTDVTDAADVVFPVAAAVEKAGTFLDWEGRSRPFEASLTDTGQLLDLHVL
                     DALADALDVHLGLPDLRTARAELEALGTRAGGVAAPSLKPGPPAKLSAGRAILATWHE
                     LLDAGSLQDATPYLAATAKPLRARLSPASARALGIADGAPLTIRGAKGGPAITLDLLI
                     TAMPDGVVWVPTRSAGSEVHRTLGVGAGAVVSLEGGSR"
     gene            6646..8022
                     /gene="nuoH"
                     /locus_tag="VII50_11785"
     CDS             6646..8022
                     /gene="nuoH"
                     /locus_tag="VII50_11785"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF004743.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HEY5051575.1"
                     /translation="MPHAAAAQIVASVIVAPDHRDIWWLIIVKTLVVFGFLMVMTLFM
                     IWAERRVVARMQQRLGPNRVGPFGILQGLADGIKLALKEDLIPSAADRIVFILAPIIS
                     AIPAFMAFAVIPFGPTVSIFGQHTNLQLTDLPVAVLFVLAMSSMGIYGIVLAGWSSGS
                     TYPLLGGVRSAAQMISYEVAMGLSFVGVFIQSGSLSTSEIVAAQQQRWFCLTASVSFL
                     IYAVAMVGETNRAPFDLPEAEGELVGGFHTEYTSLKFALFFLAEYINMVTVSALMTTL
                     FLGGWRAPVPISWWHAANTGWWPVLWFFGKLFAVLFVFIWLRGTLPRLRYDQFMKLGW
                     KFLIPVNLLWIMAEASIKVLDRKQLIYGIVILAIIVIAGSFVYDSADQKKRREREAEE
                     ELASLAPFDPYAGGFPVPPMPGQHLPSRRPVPIMAGGETVLPGPEIPDDEPDGPGGSS
                     GPGEVIDG"
     gene            8015..8560
                     /gene="nuoI"
                     /locus_tag="VII50_11790"
     CDS             8015..8560
                     /gene="nuoI"
                     /locus_tag="VII50_11790"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HEY5051576.1"
                     /translation="MASFFGPVKGFGVTFATMFKPVNTELYPEQTKVTMPRFHGRHVL
                     NRHPDGLEKCVGCELCAWACPADAIYVEGADNTDEERFSPGERYGRVYQINYLRCILC
                     GLCIEACPTRALTMSNEFEMADDNRADLIFTKEQLLAPLAPGMELPPHPMRLADAKDY
                     YRGTPAAHVMIGDGDAEEVKD"
     gene            8560..>8751
                     /locus_tag="VII50_11795"
     CDS             8560..>8751
                     /locus_tag="VII50_11795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5051577.1"
                     /translation="MPTAILAASSMHSGGPETAIFWIFAPLSVLAALGMVFSRNTVHG
                     ALMLGGVMLSLAVFYAAEDA"
BASE COUNT         1435 a         2845 c         2878 g         1593 t
ORIGIN      
        1 cgcattcagg tcatgaagct tggcccgaac gccaagaagc aagcgcagga gcgggagatc
       61 gaggcgcttg aggccgtgcc gacccatcaa atgaagggtc tgcttcggtg agcgatcagc
      121 cggtgggtca accgccgacg gcgcccgtga tgagcggagc cgaatccgag atcggcgcgg
      181 tccctgcgca gtccacaggg ccagcgccct cgacgtcacg cccctacggc gggtacttcg
      241 acgaggtcgc cgatgcgctt gcccaggcct acccaaacat cgcgcaggcg atcgagcgat
      301 cgattgtcga ccgcggcgag ctgacgctgc acgtgcgcag ggagaggctc cgcgagcttg
      361 cccagcagtt gcgcgacgat caggacctcc gctttgaact gctgtcgtcg ctgtccggtg
      421 tgcactatcc gatggacgcc ggccgcgagc tgcatgttgt gtacgagctg acgtcaatga
      481 cgcacaaccg ccggatccgg ctcgaggttg cagcgccgga cgacgatccg catgtgcctt
      541 cagtggtggc ggtctggccc acggccgact tccaggaacg tgaggcctgg gacatgttcg
      601 gggtgatctt cgatggccat cctgcgttga cgcgcatcat gatgcccgac gactggcccg
      661 gccatccgca gcgcaaggac tacccgctcg gtggtatcgc ggtcgagtac aagggcgcca
      721 ctgtgccgcc tccagatgag cggagacagt acgcatgacg acgtcgactg atccttacgc
      781 aggtgtgcgc gagacgaccg agggccgcgt ctacacggtg accggtggcg actgggacga
      841 cgtcgcggcc gatgtagcga gcgaacacgc ggagcgggtc gtcgtcaaca tgggtccgca
      901 gcacccgtcg acccacgggg tgctgcgcct gatcctcgaa ctcgacggcg agaccatcac
      961 gcaggcccgg ctcgccatcg ggtatctgca cacgggtatc gagaagaact gcgaataccg
     1021 caacttcact caagctgtga cgttccttac ccgcgctgac tatctgtcgc cactgttcac
     1081 cgagtcgggc tactcgatgg cggtcgagaa gttgctcggc attaccgacg aggttccgat
     1141 tcgcgcgcag gtgatccgcg tgatgatgat ggagctcaac cggatctcct cgcacctggt
     1201 ctggctcggc accggcggtc tcgaggtcgg cgcgctgtcc gcgatgctct acgcgtttcg
     1261 cgagcgcgaa ctcatcctcg acatcttcga gatgataagt ggcctgcgga tgaaccacgc
     1321 atacgtccgt ccgggcggcg tcatccagga tctgccacct ggggcggtcg agaagatccg
     1381 cgagttcgtt cagctcatgc ctgggcgcat cgatgagtac cacaatctgc tgaccggaca
     1441 gccgatctgg atcgcccgcc tgcagggtgt ggggtatctc gacgtcaatg cctgcgtcgc
     1501 cctcggcgtc accggcccgt tacttcgcgc ggccggcctg ccgtgggacc tccgcaagag
     1561 ccagccgtac agcggttatg agacgtatga gttcgacgta ccaacgcaac aggaaggcga
     1621 ctgcttcgcg cgttatctgg tccggatgga tgaaatgcgc gagtccgtca acatcatcga
     1681 gcagtgcctc gatcgcctgc gcccaggccc ggtcatggtc gaggacaaga agatcgcgtg
     1741 gccggcccaa ctggcactcg gtgcagacgg catgggcaac tcgctcgacc acatccggca
     1801 catcatggcg acctcgatgg agtcgcttat ccatcacttc aagttggtca ccgagggatt
     1861 ccacgtaccg cccggccagg tctacgtgcc gatcgaatca ccgcgcgggc aactcggcgt
     1921 ccatctggtc agtgacggtg gcaccaagcc gtatcgagtg cacctgcgcg agccatcgtt
     1981 cgtgaacctg caggcggtac cggcggtatg cgagggcagt ctcattgccg acgtcatcgc
     2041 gtcagttgcc agcctcgacc cggtgatggg cgggtgtgac cgttgagtca tagccatggc
     2101 gagcgcagcg gcttgccgac cgtcacgctc gacccacggc tcggcccagg cgcatcgctt
     2161 gctgatccgt tgtccgacga cgcgaaggcg cgggtccgcg atctcggcgc gcaggtcatc
     2221 gcgagatacc cgcggccgcg gtcggccatg atgccgctgc tctatctggt gcaggccgag
     2281 caaggctacg tgtcacctga tggcatcaag ctggttgccg agctgctcgg actcacgacg
     2341 gccgaggtgg ccgcggtgag caccttctac acgatgtaca agcggcgcag ttgcggcgac
     2401 tatcacgtcg gcgtctgcac gaacactttg tgcgcgatcc ttggtggcga tgccattttc
     2461 gctgcgctca aagagcatct cggcgtcggc aacgccggca cgacgcctga caacaaggtg
     2521 acgcttgagc acatcgagtg caacgcggcg tgcgactacg ccccggtcgt gatggtcaac
     2581 tgggagttct tcgacaacca gaccgtcgag agctcgaagg aactcgtcga caggctgcgc
     2641 gcgggcgacc tcccgatgcc gacgcgcggc gcacctctcg tcacccatgt cgagacatcg
     2701 cgtgtgctgg ccggctttcc cgacgcgttg gtcgacgaag gtccctccgc aggggccgag
     2761 tcgcttgtcg gtctcgagct ggcgaaggag cgcggcgaga cggccccggc gctcgagagc
     2821 aaggacccgt catgaccttc gcgctgacgc cggtccttac ggcgaactgg ggcacgcctc
     2881 ggtcctggac gctcgcctcg taccgtcgcg ccggcggcta cgactcactg cgcgccgcgc
     2941 tggcgatgga gcccgacgct ctcattgcta tggtcaagga ctccgggctt cgggggcgtg
     3001 gcggcgccgg tttcccaacc ggtatgaagt ggggcttcat cccgcagggt gacggcaagc
     3061 cgcactacct ggtcgtgaac gcagacgaga gcgaacccgg tacctgtaaa gacattccgt
     3121 tgatgatggc cagcccgcac accctgctcg agggcgtgct gatctcggcc tacgcgattc
     3181 gcgcgaacca ggcattcatc tacgtccgcg gcgaggtgct gcacgtcgtc cgccggttgc
     3241 tcaatgcagt cgaagaggcg aaggcagccg gcttcatcgg gcacaacgtc ctcggatcgg
     3301 gatttaacgt cgacgtcgtc gtgcacatcg gcgcgggtgc gtacatctgc ggcgaagaga
     3361 ccgcactact cgaatcgctc gaaggattcc gtggccagcc acggttgcga ccgccgttcc
     3421 ccgcgatcga aggcctctac gcctgcccga ccgtcatcaa caacgtcgag accatctcta
     3481 gtgtcccggc aatcgtgcgt gggggtgccg cctggttcaa gaagatgggc acagagaagt
     3541 cgcccggatt caagatcttc tcgctctccg gccatgttgc gcggcctggc cagtacgaag
     3601 cgccgctcgg tatcaccttg cgcgagctgc tcgacgccac cggcggaatg cgcgagggtc
     3661 acgaactaaa gttctggacg ccggggggct cgtcgactcc gttgtttacc gccgagcacc
     3721 tagacgtccc gcttgatttc gaagggccac cggcgcacgg ctcgatgctt gggacgaccg
     3781 cccttcagct cttcgacgag acgacgtgtg tcgtgcgggc agtgttgcgg tggagtgagt
     3841 tctacgcgca cgagtcctgc ggtaagtgca cgccatgccg cgagggagtc ttctggtacg
     3901 tccagatcct cgaacgactt gagcgtggtg agggcgacga gcaggacatc gaaacgctgc
     3961 tcgacctctc cgagaacatt ctcggccgcg cgttttgtgc tctcggcgac ggcgcgacca
     4021 gcccggtgac ctcgtcgatc caatatttcc gtgacgagta catcgcccac ttcgacgggc
     4081 gcggctgtcc gttcgacccg gtcgcgtcga ccgtcttcgc cggggcgagc gcctgatgac
     4141 cgtgacgacg aatccgccag ccggcgccgg aggcggcgaa aggcacagcg ccggcggcgc
     4201 gaaccagccg gctcggtccg acctggtcac gctcaccgtc gatgggttcg agatttcggt
     4261 gcccaagggc acgctggtga ttcgtgcggc cgagttcctc ggcatccagg taccgcgctt
     4321 ttgtgagcac ccgctgcttg acccggtcgg tgcctgccgg cagtgcctcg tcgaggtcga
     4381 gggtcagaag aagccgatgg cgtcatgcac gacgacggtc accgagggca tggtcgtgaa
     4441 gacgcagatc acctcggccg tggccgagaa ggcgcagaac ggcgtcatcg aactcctgct
     4501 cgtcaaccac ccgctcgact gcccggtgtg cgacaagggc ggcgagtgtc cgctgcagaa
     4561 ccagacgatg acgaacggct actccgagtc gcgtttccgc gacgtgaagc ggacgtatcc
     4621 caagccgatt gcgatctcag cgcaagtact tctcgaccgc gaacggtgcg tgctctgcac
     4681 gaggtgcacc cggttcgccc agcagatcgc cggcgatccg ttcatcgaga tgcttgagcg
     4741 ttctgcgctc gaacaggtcg cggtttacga ggatcagccg ttcaactcct acttctcggg
     4801 caacaccgtg cagatctgcc cggtcggcgc gctgaccgga acccaatatc ggttccgagc
     4861 ccggccgttc gacctcgtgt catcgccgag catctgtgag cactgtgcct ccggctgcca
     4921 gcaacgcaac gatcaccggc gcggcaagat gctgcgccgg ctggcgggcg tcgatccgca
     4981 ggtcaacgag gagtggaact gcgacaaggg ccggtgggcc tttagctaca ccaccgcgtc
     5041 cgaccggctc gacacgccgc tggttcgcga cagcgacggc gacctcgttc ccgcatcgtg
     5101 gccggaggcg ttgcgtgccg ctgccgcagg cctcgcggca gcgcggaacg gagcaggcgt
     5161 cggtgtgctc gccgggggac gcctcaccgt cgaagatgcc tacgcctacg cgaaattcgc
     5221 ccgagttgcg ctgggcacga acgacatcga ctttcgggcg cgggtgcact ccgacgaaga
     5281 actcgcattt ttggggtcgc gcgttgccgg tcgcttcctc gacgtgacct acgacgacct
     5341 cgaagcagcg tcgactgtcc tgctcgtcgg gctcgagccc gaggaggagt cgccgatcat
     5401 cttcttgcgg ctgcgcaagg cggcgctgaa gaagcacgcg aaggtcgtgt cgattgcgcc
     5461 ttttgcgacg cgtggtctcg acaagatggg cggccgattg atcgcgacgg ttcccggtgg
     5521 cgaagcgacg atgttgcagg tgcttgcatc gggtgagacg gtcgaggtcc ctcctggtgt
     5581 cgatgccagc ggttggtcgg agctcctcac cgaggtggcg gccggcctcg cgcttccgtc
     5641 gagtgtgatc cttgtcggcg agcgacttgc gggtgtgact ggcggtcttt ctgccgcagc
     5701 cgcgttggcc gacgcgaccg gcgcccgact cgcctgggtc ccccgccggg ccggcgagcg
     5761 aggtgctgtc gaggcaggtg cactaccgaa cctcttgccg ggtggtcgcg ccgtcaccga
     5821 caacgacgcg cgcggtcagg tcgggctgca atgggtgacc accattccgg ccaagccggg
     5881 ccgcgacact gctggcattc tcgaagccgc ctcgcgtggc gagttgggtg cactcgtcgt
     5941 ggccggtgtc gacctcgacg acctgcccga tccggccgcg gcgcgcgcag ccatcgacgc
     6001 agccagcttc gtggtcagcc tcgagctgcg gcgcaccgat gtgaccgacg ctgccgacgt
     6061 cgtcttcccc gttgccgcgg ccgttgagaa ggccggcacg ttcctcgact gggagggccg
     6121 ctcgcgaccc ttcgaggcgt cgctgaccga caccgggcaa ctgctggatc tccacgtgct
     6181 cgatgctctg gccgatgcac tcgacgtcca tctcggattg cctgatctgc gcacggcgcg
     6241 ggccgaactc gaagcgcttg gcacccgcgc cggtggggtg gcggctccat cgttgaagcc
     6301 cggaccgccc gcaaaactca gtgcaggtag ggcgatcctc gcaacgtggc acgagttact
     6361 cgatgccggc agccttcaag acgcgacgcc gtacttggcc gcgacggcca agccattgcg
     6421 ggcccggttg tcgccggcca gcgcgcgggc actgggcatc gccgacggcg ccccgctgac
     6481 cattcgcggc gcgaagggcg gtccggcgat cacgctcgac ctgctcatca cagcaatgcc
     6541 ggacggcgtg gtgtgggtgc ccacccgatc ggctggcagc gaggtgcacc gtaccctcgg
     6601 tgtcggcgcc ggcgcggtcg tcagtctcga agggggctcc cggtgatgcc gcacgctgca
     6661 gcggctcaga tcgtcgcgag tgtgatcgtc gcgcctgacc accgcgacat ctggtggctg
     6721 atcatcgtca agaccctcgt ggtgttcgga ttcctcatgg tcatgacgct gttcatgatc
     6781 tgggccgaac gccgagtggt tgcccgtatg cagcaacgcc tcggcccgaa ccgggtcggc
     6841 ccgttcggca tcctgcaagg cctcgccgac ggcatcaagc tcgcgctcaa agaagacctc
     6901 atcccgagcg cggccgaccg gatcgtgttc atcctggcgc cgatcatctc ggcgatcccg
     6961 gccttcatgg cgtttgccgt tattccgttc ggcccgacgg tctcgatttt cggtcagcac
     7021 acgaacctgc aactgaccga ccttccggtt gcggtgctgt tcgtgctggc gatgagttcg
     7081 atgggcatct acggcatcgt gctcgccggc tggtcgagtg gctcgaccta cccgttgctg
     7141 ggcggcgtgc gttcggcggc ccagatgatc agctacgaag tcgcgatggg gctgtcgttc
     7201 gtcggcgtct tcatccagtc gggatcgttg tcgacctccg agatcgtcgc cgcacaacag
     7261 cagcgctggt tctgccttac cgcgtcggtg tcgttcctga tctatgcggt cgcgatggtc
     7321 ggtgagacca accgggcgcc gttcgacctg cccgaggccg agggcgagct cgtcggcggg
     7381 ttccacaccg agtacacctc gttgaagttc gcgctcttct tcctcgccga atacatcaac
     7441 atggtcacgg tgtcggcgct gatgacgacc ttgttccttg ggggctggcg ggcgccggtg
     7501 ccgatctcgt ggtggcatgc cgccaacaca ggttggtggc cggtgctgtg gttcttcggc
     7561 aaacttttcg ccgtcctgtt cgtcttcatc tggttgcgcg gaacgctgcc gcggctgcgc
     7621 tacgaccagt tcatgaagct cggctggaag ttcctcattc cggtcaactt gctctggatc
     7681 atggccgagg catcgatcaa ggtgctcgac cgcaagcagc tcatctacgg gatcgtgatc
     7741 ctggcgatca tcgtgatcgc gggctccttc gtctacgaca gtgcggatca gaagaagcgg
     7801 cgcgaacgcg aggccgaaga agagctcgcc agtctggcgc cgttcgatcc ctatgccggc
     7861 gggttcccgg tgccgccgat gccgggtcag cacctgccct cgcggcgtcc cgtcccgatc
     7921 atggccggcg gcgagacggt ccttcccggc ccagagattc ccgatgacga gcccgatggt
     7981 ccgggcggct cgtccggtcc tggcgaggtc atcgatggct agtttcttcg ggccagtcaa
     8041 gggtttcggt gtcaccttcg ccacgatgtt caagccggtg aacactgagc tttatcccga
     8101 gcagacgaaa gtcaccatgc cgcgctttca cgggcggcac gtgctcaacc ggcaccccga
     8161 cggcttagag aagtgcgtcg gttgcgagct atgtgcctgg gcgtgtcccg ctgacgcgat
     8221 ctacgtcgag ggcgcggaca acaccgacga ggagcgtttt tctcccggcg agcgctacgg
     8281 ccgcgtctac cagatcaact acctgcggtg catcttgtgc ggcttgtgca tcgaggcgtg
     8341 cccaacgcgc gcgctgacta tgagcaacga gtttgagatg gccgacgaca atcgcgccga
     8401 cctcatcttc accaaggaac aactgctggc gcccctggct ccgggcatgg agttgccgcc
     8461 gcatccgatg cggctggccg acgccaagga ctactaccgc ggcacgccag cggcgcacgt
     8521 catgatcggc gatggcgacg ccgaggaggt gaaggactag tgcctacggc gatcctcgcg
     8581 gcgagcagca tgcacagcgg tggcccggaa accgcgattt tctggatctt cgctccgctg
     8641 tcggtactcg cagccctggg catggtgttc tcccgcaaca ccgtgcacgg cgcgctcatg
     8701 ctcggtggtg tgatgctgtc gctggccgtg ttctacgcag ccgaagacgc g
//
LOCUS       DATCBJ010000586         2293 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_458602, whole genome shotgun sequence.
ACCESSION   DATCBJ010000586 DATCBJ010000000
VERSION     DATCBJ010000586.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2293)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2293)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2293
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_458602"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            60..1271
                     /locus_tag="VII50_11800"
     CDS             60..1271
                     /locus_tag="VII50_11800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5051578.1"
                     /translation="MSAATDGDVGVVHFSIRRPDGRRRSTHMRGLGLLAAWAAGWFVV
                     LERHGGYSWHYFVHGSSLLFGGHPAGLTGAGGLQLYGSYPQYQIGPLAFVVAAGLGPF
                     GLVVSQLFMVALGGVVLLSGARVAVLVGATGDWRRRLRYAAALFVPLWMELAVHYSHL
                     DDALAVALASASLWAAAARRGWAGVLLGGAIAAKPWAAGFLPLLWALPPGQRRSGVLW
                     AVSVPLFAWLPFAVADPGMVWSTSHFTIQNASDSALRVLGVHAARTPGWCRPAQLLCG
                     VTMAALAVRRGRWPAVVLASVAVRLLLDPGTYDYYTAGALAGALIVDVALERRRWPLA
                     STVAFAGLYAPHLAHLPAQLCGESRVVTCVALLALAVAGRRADGRSVEAAHRPTRHAV
                     GESLGVPSGAL"
     gene            1280..1927
                     /locus_tag="VII50_11805"
     CDS             1280..1927
                     /locus_tag="VII50_11805"
                     /inference="COORDINATES: protein motif:HMM:NF020893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HEY5051579.1"
                     /translation="MQHLLSTPAVVVYVAVGLLVFAEDAFFVGFVLPGETAAILGGVD
                     ASRHRVNLWIVLIVVVAAEIVGDSVGYQIGRRFGPRVIDLPVLRRHRARIDDARELLA
                     RRGGWAVFAGRSVAFFRAVVPALAGVAHMPYRRFLAYNAAGGLVWGVSAVMVGFLAAN
                     SYQHVASAMGRGAAIAIGCLVVAAVIGWRIHSHKHDHATAGRACTTCRPTPKTPQ"
     gene            complement(2029..>2293)
                     /locus_tag="VII50_11810"
     CDS             complement(2029..>2293)
                     /locus_tag="VII50_11810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5051580.1"
                     /translation="DDVGSGVGSADADVVQFAGVAHGIGRTGRPATSAALILFLSVVS
                     MSTMPTRSPTATPIADTGALASTRVKLASSRNVERTTPTLWIR"
BASE COUNT          271 a          708 c          838 g          476 t
ORIGIN      
        1 gcgcaccggt gcgtgctcac gtcaggtgat tttcaggttc gtccggtatc caggagggtg
       61 tgagcgctgc gaccgatggc gacgttggtg tcgtccactt ttcgatccga cgcccggatg
      121 ggcgccgacg atcgacgcat atgcgagggc tggggctgct cgctgcgtgg gcggcagggt
      181 ggttcgtggt gttggagcgt cacggcggct attcgtggca ctacttcgtg cacggttcat
      241 cgttgttgtt cggcggtcac ccggctggcc ttacgggtgc aggagggctg cagctgtacg
      301 gcagctaccc gcagtatcag atcggtccgc tggcgttcgt cgtcgccgcg ggtttgggcc
      361 cgttcgggct ggtcgtctcg cagctgttca tggtcgcgct tggcggtgtc gttttactga
      421 gcggcgcgcg agtcgcggtg ctggtcggag ccactggaga ttggcgacgg cgcttgcggt
      481 atgccgcagc gctctttgtc ccgctgtgga tggaacttgc tgttcattac tcccaccttg
      541 atgatgcgct ggctgtcgcg cttgctagtg cttcgctttg ggcggcggcg gcgcggcgcg
      601 ggtgggcggg tgtgctgctg ggaggcgcca tcgcggcgaa gccctgggcc gccggctttt
      661 tgccgctgct gtgggcgttg ccgccaggcc agcggcggtc gggagtgttg tgggcggtct
      721 cggtgccgct ctttgcgtgg ttgccgttcg cggtggctga cccgggcatg gtgtggtcga
      781 ccagtcattt caccattcag aacgcatcgg actctgcgtt gcgggtcctt ggagtacatg
      841 ccgcgcgtac gccgggatgg tgtcggccgg cgcagttgct gtgtggtgtg acgatggctg
      901 cgctggccgt gcgccgcggc cgctggcccg ccgtggttct cgcgtcggtg gctgtccggc
      961 tgctgctaga tcccgggacg tacgactact acaccgcagg tgcgttggcg ggtgcgctga
     1021 tagtcgatgt cgcgctggaa cgccgtcgct ggccgctggc gtcgaccgtc gcgttcgcgg
     1081 gcttgtatgc cccgcacctg gcgcacctgc cggcccaatt atgtggtgag tcgcgcgttg
     1141 tgacgtgcgt cgctctgctg gctctggcag ttgcgggccg ccgtgccgac gggcgctcgg
     1201 tcgaggcagc ccaccggccg acccgccacg cagtcggaga atccttgggc gtgcccagtg
     1261 gtgcattgtg acgggcctaa tgcagcattt gctgtccact ccggcggtgg ttgtctatgt
     1321 cgcagtgggc ttgctcgtat tcgccgaaga cgcattcttt gttgggttcg tgctgcccgg
     1381 tgagaccgcg gccatcctgg gcggcgtcga tgccagccgg catcgggtga acctttggat
     1441 cgtgttgatc gtcgtcgtgg ccgcggagat cgtcggagac agcgtcgggt accagatagg
     1501 tcgccggttc gggcctcggg tcatcgattt gccggttctg cggcggcacc gtgcccgcat
     1561 cgatgatgcc cgtgagctcc tcgcccgcag gggcggctgg gcggtgttcg ccggccggtc
     1621 ggttgcgttc ttcagggcgg tggtgccggc ccttgccggg gtcgcgcaca tgccctaccg
     1681 gcgattcctg gcatacaacg cggcgggcgg acttgtgtgg ggtgtcagtg cggtcatggt
     1741 cgggttcctt gctgccaact cctaccagca cgtggcctcg gccatgggac gaggtgccgc
     1801 gatcgccatc ggttgtctcg tcgtcgctgc ggtcatcgga tggcggatcc actcgcacaa
     1861 gcacgatcac gcgaccgccg gccgggcatg cacaacctgc cggccaaccc ccaagacgcc
     1921 gcagtgacga gcgtcgaccc cgccccgcgc cgtcccctcc cgggacctga cctacgctgc
     1981 cgatgagcat gctgcgcaac cctgttcgca cagcccgcgc ggtgctccct agcgaatcca
     2041 gagagtgggc gtggtgcgct ccacgttccg actcgaagcc agtttgactc gggtcgaggc
     2101 cagcgcgccg gtgtccgcga tcggagtggc tgtcggcgat cttgtgggca tagtggacat
     2161 cgagacgacg gacaggaaca ggatcagggc ggcgctcgtg gcaggtcggc cggtgcggcc
     2221 gatgccgtgc gcaacgccgg caaactgcac cacatcggca tcggccgaac ccacgcccga
     2281 acccacgtca tcg
//
LOCUS       DATCBJ010000587         3897 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_495993, whole genome shotgun sequence.
ACCESSION   DATCBJ010000587 DATCBJ010000000
VERSION     DATCBJ010000587.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 3897)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3897)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3897
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_495993"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..209
                     /locus_tag="VII50_11815"
     CDS             <1..209
                     /locus_tag="VII50_11815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009072664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HEY5051581.1"
                     /translation="GADLAGMSTTLEAIAAREAGAEVLGISLVTNLAAGMTGEPLDHK
                     EVLAAGNASAARMGALLAQVVSRL"
     gene            206..1858
                     /locus_tag="VII50_11820"
     CDS             206..1858
                     /locus_tag="VII50_11820"
                     /EC_number="5.4.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF014877.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HEY5051582.1"
                     /translation="MNFEAAARTWLAEDPDSDTRAELSELLDSRDAEGLSDRFATRLQ
                     FGTAGLRGALGAGPNRMNRAVVIRAAAGLAAYLLETGGTSVVIGYDARHKSDVFAWDT
                     AAVMAGFGLTAWVLPRQLPTPVLAFAIRHLGVSAGVMVTASHNPPQDNGYKVYLGDGG
                     QIVPPADADISRHIDAVGALADVPRSDAWQLLDDEVLDAYLAGITSMPLLAALPHRDV
                     RIAYTPLHGVGRDVVLAAFARAGFTSLSVVAEQGAPDPDFPTVAFPNPEEPGAIDLAL
                     ALARATSADLVLANDPDADRCAVAILDSAVAGGWRMLRGDEVGVLLADFLLSHGISGR
                     YATTIVSSSLLSKMADRHGMPYSETLTGFKWIVHAGSDLVYGYEEALGYCVAPYLTRD
                     KDGISAALIVALLAATLRASGSSLVERLDGLAREYGLHATDQVSVRVSDLALIGAAVD
                     RMRSAPPSMLGGLPVTGVDDLSLGGAGLPPTDGLRYWLDGTALRCGPARVVVRPSGTE
                     PKLKCYLELVVEVTGSIDDARVLARGVLHEIGTDLRAALGLG"
     gene            complement(1868..2854)
                     /locus_tag="VII50_11825"
     CDS             complement(1868..2854)
                     /locus_tag="VII50_11825"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEY5051583.1"
                     /translation="MTDPTRWGILSTALINAKVLKGAALSADVSVVAVGSRAGDRSRE
                     FASHWGIARAYGSYDEILADPDVEAVYIPLPNSMHHTWTMRSLEAGKHVLCEKPYSRH
                     PAEVTAAFDEAARRGLVLSEAFMFRYHPQTQALVQLVTSGQLGQLGLISGAFSGPTKD
                     PGNVRLDPLLDGGSLMDVGVYPLSAARLLAGEPNVVTAQQIKGVTGVDVGFTATLRFD
                     DDVLGHFDSGFHLPDRSRLEVVGSLGRVEVSDPWHCAEPALTVTMHGAEPLRIPVEHA
                     NSYQLELEEFGRAVRGEPNQLLGRADAEGQATAIHALYLAADSDRTVTIGNQ"
     gene            complement(2851..>3897)
                     /locus_tag="VII50_11830"
     CDS             complement(2851..>3897)
                     /locus_tag="VII50_11830"
                     /inference="COORDINATES: protein motif:HMM:NF012245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HEY5051584.1"
                     /translation="RIGRIATQTHILALNASIEATKNVTEGRGFAVIADSVRTLASES
                     AASAVTINTTLSALAASLDVFRSDSDAADVHARSVQASTDAMSEFISELTGRAARVSD
                     SAQTIEATANASEEGTSHFVDSLSRLTEGIDRSQKEIARSRDRVASLLGSANVVLQDL
                     SASGVRTVDTPYIEAAVDAAARARAILEDCVARGAITREDLFDENYVPIPGTDPVQVL
                     TKFTGFAEQNLLPLMESVHANLSDSVYCVAGDRNGYAPANTAEYSHPQGPDPAWNDAH
                     CRNRRIFDNEVATRAAKNRAGPIIQVYRRDLGGGTFDIVKDAAAPIFVGNDHWGAFRI
                     GYIAGAPDVGVTSR"
BASE COUNT          605 a         1249 c         1304 g          739 t
ORIGIN      
        1 tgggcgctga cctggcgggc atgtcgacca cactcgaagc gatagccgcg cgcgaggccg
       61 gcgccgaggt gctcggcatc tcgctggtga ccaacctcgc tgcgggcatg acgggcgaac
      121 cccttgacca caaggaggtt ctcgccgccg gaaacgcgtc cgccgcccgc atgggtgcgc
      181 tactggcgca ggtcgtgtcg cggctgtgaa cttcgaagcg gctgcgcgga cctggcttgc
      241 cgaagacccg gactccgaca cgcgcgccga gctatcggaa ctgcttgatt cgcgggacgc
      301 cgaggggctg agcgaccggt tcgctacccg gctgcagttc ggtacggcag gattgcgcgg
      361 cgcgcttggc gccggcccga atcggatgaa tcgcgcggtc gtcatccgcg ccgccgccgg
      421 gctcgcggcg tacctgctcg agaccggcgg cacatcggtc gtcatcggct acgacgcgcg
      481 gcacaagtcc gacgtgttcg cgtgggacac cgcggccgtg atggctggat tcgggctgac
      541 tgcgtgggtg ctgccacggc aactcccgac gccggttttg gcattcgcga tccgccatct
      601 cggcgtttcc gccggcgtca tggtcaccgc gtcgcacaac cctccgcagg acaacggcta
      661 caaggtctac ctcggtgacg gcggtcagat cgtgcctcct gcggacgccg atatctcgcg
      721 gcacatcgac gctgtcggcg cgctggccga cgtcccgcga agcgatgctt ggcagctact
      781 ggacgacgag gtgctcgacg cgtacctggc cggcatcacg tcgatgccgt tgctcgcggc
      841 gttgccgcat cgcgacgtgc ggatcgcgta cacgccgcta catggggtcg gtcgtgacgt
      901 ggtgcttgct gcttttgcgc gcgcgggctt tacatcgttg tcggtcgttg cggagcaggg
      961 tgcccccgac ccggactttc cgaccgttgc gttcccgaac cctgaagagc ccggcgcgat
     1021 cgacttggca ttggcgttgg cgcgggccac gtcggccgat cttgttcttg ccaatgaccc
     1081 ggacgccgac cggtgtgccg tcgcgattct cgattccgcg gttgccggtg gctggcggat
     1141 gctgcgcggg gacgaggttg gtgtgcttct agccgatttc cttttatcgc acgggatttc
     1201 cggccggtac gcgacgacca tcgtgtcgtc gtcgttgctg tcgaagatgg ctgatcggca
     1261 cggaatgccg tactccgaaa cgctgaccgg cttcaagtgg atcgttcacg cgggttcgga
     1321 tctcgtgtac ggctatgaag aggcgctcgg gtattgcgtt gccccgtact tgactcgcga
     1381 caaggacggc atcagcgccg cgttgatcgt tgccctgctt gcagcgacgc ttcgtgcctc
     1441 cgggtcgtcg ctcgtcgagc gccttgacgg tctcgcacgt gagtacgggc tgcacgcgac
     1501 cgaccaggtg tcggttcggg tatccgacct cgcgctcatc ggcgccgcag ttgaccggat
     1561 gcggtctgct ccgccgtcca tgctcggggg cttgccggtg accggtgtcg acgacctgtc
     1621 gctcggcggt gcggggctgc caccgaccga cggcctgcgc tactggctcg acgggactgc
     1681 actgcgctgt ggaccggccc gcgtcgtggt ccggccgagc ggaaccgagc ccaagctgaa
     1741 gtgctacttg gaactcgtgg tcgaggtcac cggttcgatc gacgacgcgc gggtgttggc
     1801 gcgcggtgtg ctgcacgaaa tcggcaccga cctgcgagcc gcactcggcc ttggctgacg
     1861 cccgctgtta ctggttgccg atcgttacgg tgcggtcgga gtcggccgca agatacagcg
     1921 catggattgc cgtcgcttgt ccctctgcat cggctcggcc aagaagctgg ttcggctcgc
     1981 ctcgaacggc gcgcccgaac tcctcgagtt caagctggta ggaatttgcg tgctcgactg
     2041 gaatccgtaa cggttcggcg ccatgcatcg tgacagtgag agcaggttcg gcgcagtgcc
     2101 aggggtcgga cacctctacg cgaccgagcg agccgacgac ctcgaggcga ctccgatcgg
     2161 gaaggtggaa cccgctgtcg aaatgaccaa ggacgtcgtc atcgaaacgc agagtcgcgg
     2221 tgaagccgac gtctactccc gtgacgccct ttatctgctg ggcggtcacc acattcggct
     2281 ccccggccag gagcctcgca gcgctgagcg ggtacacacc gacgtccatc aggcttccgc
     2341 catcgagcag cggatcgagc cgcacgttgc cggggtcctt ggtaggcccc gagaatgccc
     2401 cagagatgag acccagttgg cccagctgac cacttgtcac gagctggaca agggcttgcg
     2461 tctgcgggtg atatcggaac atgaacgcct cgctgagcac caacccccgt cgtgccgcct
     2521 cgtcgaaagc agcggtcacc tcggcgggat ggcggctgta gggcttctcg cacagcacat
     2581 gcttgcccgc ttcgagcgac ctcatggtcc aggtgtggtg catcgagttg ggcagcggaa
     2641 tgtagacggc ctcgacgtcc ggatcggcca agatctcgtc gtagctgccg taggcgcgcg
     2701 cgatgcccca gtgactcgca aactcacggc tgcggtcacc agcgcgactg ccgaccgcaa
     2761 ccacactcac atctgctgac agtgccgcgc ccttgagcac tttggcgttg atgagcgccg
     2821 tcgacaggat tccccaacgc gtgggatcgg tcatcgcgaa gtgaccccga catcgggggc
     2881 gccagcgatg taaccgatcc ggaacgctcc ccagtggtcg ttaccaacaa agatcggcgc
     2941 ggccgcatcc ttcacgatgt cgaacgtgcc gccgccgagg tcgcggcggt agacctggat
     3001 aatcgggccc gcgcggttct tggccgctcg agttgcaacc tcgttgtcga agattcggcg
     3061 attccggcaa tgcgcgtcgt tccacgccgg gtcggggcct tgtggatggg agtactcagc
     3121 ggtgttggcg ggcgcgtagc cgttgcgatc ccctgccacg cagtacacgc tgtcggacaa
     3181 gttcgcgtgc accgattcca tcaacggcag caggttctgc tcggcgaacc cggtgaactt
     3241 tgtcagcacc tggacaggat cggtccccgg gatcgggacg tagttctcat cgaaaaggtc
     3301 ttcccgtgta attgcgccgc gcgctacgca gtcctcgagg atggcccttg ctcgagcggc
     3361 tgcatcgact gcagcttcga tgtatggagt gtcgacggta cgcacgccgg acgccgacag
     3421 gtcttgcaga acgacgttcg cggacccgag caatgacgcg acccggtcac ggctgcgggc
     3481 gatctccttc tgagagcggt cgatgccttc ggtaagccgg ctcagcgaat cgacgaaatg
     3541 cgacgtcccc tcttcgctcg cattggcagt ggcctcgatg gtctgcgccg agtccgagac
     3601 tcgtgccgct cgcccagtga gctctgagat gaattccgac atggcgtccg tgctcgcctg
     3661 cactgaacgg gcgtgcacat ctgcggcgtc gctgtcagat cggaagacgt cgagtgaggc
     3721 ggcgagcgcg ctgagtgtcg tgttgatggt tactgccgac gctgcactct ccgacgcgag
     3781 cgttcgtacg ctgtcggcaa tcacggcgaa gccgcggcct tcggtgacgt tcttcgtggc
     3841 ttcgatgctc gcattcagcg ccagaatatg ggtctgcgta gcgatgcgtc cgatccg
//
LOCUS       DATCBJ010000588         2749 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_393553, whole genome shotgun sequence.
ACCESSION   DATCBJ010000588 DATCBJ010000000
VERSION     DATCBJ010000588.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2749)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2749)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2749
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_393553"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..237
                     /locus_tag="VII50_11835"
     CDS             <1..237
                     /locus_tag="VII50_11835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5051585.1"
                     /translation="RTMSEPRTEFHNANIVSDVAEAGGCGLKNLQNGATCGLPAHHEG
                     PCDFMTPQAVPNALAEKGIDLEHGASTPIAPAAR"
     gene            complement(252..1169)
                     /locus_tag="VII50_11840"
     CDS             complement(252..1169)
                     /locus_tag="VII50_11840"
                     /inference="COORDINATES: protein motif:HMM:NF039905.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HEY5051586.1"
                     /translation="MTSVAVVAHSGKSFGGGLGELREVLAREGFPDPLWYEVSKSRRA
                     PQCARRAVADGAEVIFVWGGDGTVQRCIDALVGRAATIAILPAGTANLLATNLGVPTD
                     LVDAVQVGLRGHRRPIDTGSVNGEHFAVMAGAGFDALMIRDADGGMKDRIGRAAYVWT
                     GAKNLTASRGKATVDVDGERFFKGLVSCVLVGNVAEILGGVKAFKGARPDDGVLELGI
                     VTAKNPIQWTRTLGRVAVGKTDKSPFVRTTRGKKFKICFDRPFVYELDGGARPSTKRL
                     RIRVHPSSILICVPASAKPEIPSAVGDQS"
     gene            1580..1662
                     /locus_tag="VII50_11845"
     tRNA            1580..1662
                     /locus_tag="VII50_11845"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1621..1623,aa:Ala,seq:ggc)
     gene            complement(1790..>2749)
                     /locus_tag="VII50_11850"
     CDS             complement(1790..>2749)
                     /locus_tag="VII50_11850"
                     /inference="COORDINATES: protein motif:HMM:NF013331.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043231 - intracellular membrane-bounded
                     organelle [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDGSH iron-sulfur domain-containing protein"
                     /protein_id="HEY5051587.1"
                     /translation="NARQPAIEVTKDGPYRVSGGVDMVGADGTEEPRAEGSSHEHYAL
                     CRCGQSRNKPFCSGMHWYVNFHDPAPAEQPTVFEWVGGMPALTRMTRIFYEKYIPQDP
                     LLAPVFATMSDQHPQLVAAWLAEVFGGPKNYSEQLGGYVKMISQHLGKVLTEEKRTRW
                     IQLLVMSATDAGLPNDPEFRSVFSSYIEWGSRLAVENSQLGVTPPPHMPMPHWEWHTA
                     AGEPGSRIPAQPAGAAIVEQAVALPGPDENVLFNKHIKPLFRKQDQLSMSFVFDLWSY
                     GDVKQHVDAILDHLQKGSMPCDGAWAADKVDVFKRWHDAGMPA"
BASE COUNT          504 a          929 c          831 g          485 t
ORIGIN      
        1 cgaaccatgt ctgaaccgcg taccgagttc cacaacgcga atatcgtgag cgacgtcgcc
       61 gaggccggtg gctgcgggtt gaagaacctc caaaacggcg caacatgcgg tctgcctgct
      121 caccatgaag ggccgtgcga cttcatgacc ccgcaagcgg tgccgaacgc tctcgctgag
      181 aaaggcatcg acctagagca cggagccagc accccgatcg cccctgccgc acgctaggtc
      241 agactcggca ctcagctctg gtcgcccacg gcgctcggga tctcgggttt ggcgctcgcg
      301 ggaacgcaga tcaagatcga cgagggatgc accctaatcc gcagccgctt ggtgctaggt
      361 cgggcccccc cgtcgagttc gtacacgaac gggcggtcga aacagatctt gaacttcttt
      421 cccctggtcg tgcggacgaa cggcgacttg tcggtcttgc ctaccgcaac cctccccagc
      481 gtccgggtcc actgaatggg gttcttggcc gtgacgatgc cgagttcgag cactccgtcg
      541 tcgggccgcg cgcccttgaa ggctttgacg ccgccgagga tctctgcgac gttgcccacg
      601 agaacgcagg agaccagtcc cttgaagaag cgctcaccgt cgacatcgac ggtggccttg
      661 cctcggctcg ccgttaaatt cttggctccc gtccacacat acgcggctcg accgatgcgg
      721 tccttcatcc ctccatcggc atccctgatc atcagcgcat caaagccggc tcccgccatc
      781 accgcgaagt gctcaccgtt taccgacccg gtatcgatcg gtcggcgatg cccgcgaagt
      841 ccgacctgaa cagcgtcgac cagatcagtg ggcacaccca ggttggtcgc caacaggttg
      901 gccgtgcccg cagggaggat cgcgatggtc gcggcccggc caaccaacgc gtcgatgcat
      961 cgttgaaccg tcccgtcccc gccccacacg aagatcacct cagcgccatc tgcgaccgca
     1021 cgacgagcac actgtggcgc ccttcggctc ttcgacactt cgtaccacag aggatcgggg
     1081 aacccctcac gggccagaac ttcgcgcagt tcgcccaagc caccaccgaa gctcttgccc
     1141 gagtgagcga cgactgctac cgacgtcata cgaatctctc cgttgctgag aagctgcggg
     1201 acgttggccg gcggtcagcc tgacctggag ccaacacctc ggctgctgtg aaatacaagg
     1261 agaggagacc aggtacgggc tgatggtggt gacgcgatcg aaaatcacac cttcagcgta
     1321 gagcccgacc ggtccgcgag accgttgcga tcacctgagc ggatcgcaaa acgacgcagg
     1381 cccgcaaacg tgcagagcgg ggccgtcgcg gggccgtacg caccacgcct gtaccgcgcc
     1441 gccacgacag ggatcactcc ggcgagctgg ccacgccggt cgatcccggg cgcccgcttt
     1501 acgaaaaccc ttacacagca aggatttcca cgcacccacg gcctccacgt gccgcagaaa
     1561 cgtagatcat gggtccctag cgggccgtag gtaccacgcc agtactgcag gccccagccc
     1621 ggcaagctgg ccatgccggt tcgatcccgg tcacccgctc cactggggct accgagcgat
     1681 ctgggtgggt agcttgcccg cgatatcggg ccacccgcct tatcgctttt cggccgcctg
     1741 ggcacagcca tgctgccggt ggctgcgtgc ctccacgtga tgatcctggc tatgccggca
     1801 tgccggcgtc gtgccaccgt ttgaacacgt cgaccttgtc ggcagcccac gcgccgtcgc
     1861 acggcatgga tcccttctgc agatggtcga ggatcgcatc tacgtgctgc ttcacatcgc
     1921 cgtacgacca caggtcgaaa acgaaactca tcgatagctg gtcctgcttg cggaacagcg
     1981 gcttgatgtg tttgttgaaa agcacgttct cgtctggccc tggcaaagca acggcctgct
     2041 ccacgatcgc ggcgccagcc ggctgggctg gtatccgcga tcccggttcg ccggcggcgg
     2101 tgtgccactc ccagtgcggc atcggcatgt gcggaggcgg ggtgacaccg agctgagagt
     2161 tttccaccgc cagccgtgat ccccattcga tgtagctgga aaagacggag cgaaactccg
     2221 ggtcattcgg cagaccggcg tcggtggccg acatcaccag cagctgaatc cagcgagtcc
     2281 gtttctcctc cgtcaacacc ttccccagat gttgggagat catcttgacg tagccgccga
     2341 gttgttcgct gtaattcttt gggccgccga aaacctccgc aagccatgcc gcaaccagct
     2401 gcgggtgctg gtctgacatg gtcgcgaaga ccggggcgag cagcgggtcc tgaggaatgt
     2461 atttctcgta gaagatgcgg gtcattcggg tcaacgcagg catgccgccg acccattcga
     2521 acaccgtggg ctgctcggcc ggtgcggggt cgtggaagtt gacgtaccag tgcatgccgc
     2581 tgcagaacgg cttgttccgc gactgcccgc agcgacacaa cgcgtagtgc tcgtgcgatg
     2641 aaccttcggc gcggggctcc tcggtgccgt ctgcgccgac catgtccacg ccgccgctga
     2701 cgcggtacgg gccgtccttg gtcacctcaa tcgcgggctg ccgggcgtt
//
LOCUS       DATCBJ010000589         2186 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_237367, whole genome shotgun sequence.
ACCESSION   DATCBJ010000589 DATCBJ010000000
VERSION     DATCBJ010000589.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2186)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2186)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2186
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_237367"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..474)
                     /locus_tag="VII50_11855"
     CDS             complement(<1..474)
                     /locus_tag="VII50_11855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5051588.1"
                     /translation="MTYFVHAPSPHKSGQHLTLARRQRLERCELTMASRSLAPAFVQQ
                     VEAALSRHEWLAYGCEPHMVDEAIKILDLLDQTDRSRLQALAKTRPVGRTCHHDNPHA
                     RSRERGDHLRAIARFAKIEIKQHDVGVLMQAFIYYTVGAAPFAKVDRMDFSSLCGE"
     gene            699..>2186
                     /locus_tag="VII50_11860"
     CDS             699..>2186
                     /locus_tag="VII50_11860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HEY5051589.1"
                     /translation="MVSRDSADREGLAAARDSADGRPRSTLGGMPLEPSYGPPDGADD
                     PRVARIGRPGEYPFTRGLYRSGYRGRPWTIRQFAGFGNATQTNERYRMILDAGGGGLS
                     VAFDMPTLMGRDSDDPQARGEVGHCGVAIDSASDMEVLFQGIPLGEVTTSMTISGPAV
                     PVFCMYVAAAERQGVAHGKLNGTLQTDIFKEYMAQKEWLFTPQPHLRLIGDLMEFCAH
                     EMPDYKPLSVSGYHIREAGSTAVQELAYTLANGFAYVELGLSRHIDVDTFAPQLSFFF
                     DAHVDFFEEIAKFRAARRIWAHWMRDVYGAKTERAQWLRFHTQTAGVSLTAQQPDNNV
                     VRTAVEALAAILGGTNSLHTNALDEVLALPSERAASIALRTQQVLLEETGLTNVVDPL
                     GGSWFVEALTDQIEAEAEKIFTQISALGGDGSMMAGVLRGIEDGWFTGHIAEAAYRQQ
                     QEFESGERRIVGVNSYTDDFEPPLEIMRVSHEVETEQCATLAARRA"
BASE COUNT          376 a          674 c          766 g          370 t
ORIGIN      
        1 ctcaccgcag agggagctga agtccatgcg gtcgaccttc gcgaacgggg cggccccgac
       61 ggtgtagtag atgaatgctt gcatcaacac cccgacgtcg tgttgcttga tctcgatctt
      121 ggcgaagcgg gcgatggcgc gcagatgatc gccccgctcg cggctgcggg cgtgcgggtt
      181 gtcgtggtga caggttcgac cgaccgggcg cgtcttggcg agtgcttgga ggcgggagcg
      241 atcggtctga tcgagaaggt cgaggatctt gatcgcctcg tcgaccatgt gcggctcgca
      301 gccgtaggcg agccactcgt gtcggctcag cgccgcctcg acctgctgaa cgaatgcagg
      361 cgccaacgaa cggctcgcca tcgtcagctc gcaccgttcg aggcgctgac ggcgcgcgag
      421 tgtgaggtgc tggccgctct tgtgcgggga cggcgcgtgg acgaagtagg tcacgaactc
      481 aacgtctgcg aagcgaccgc gcgcacgcat gtgcgcggca tcttgcgaaa gctcggtgtg
      541 aagtcacagc tcgccgccgt cgcgcgcgct cgggaagtgg gctggcagga ggaggagtct
      601 gcccggctgg cgccgagatc ggcgtaaagc gggtggcgtg gccgcgcgcc gaagtatttg
      661 gacgtcctac tatcgaggga ggacggctgg aggcaggcgt ggtgtctcgg gatagcgcgg
      721 atcgcgaagg cctcgcggcc gcgagagaca gtgccgacgg tcggccacgg tcgacgcttg
      781 gcgggatgcc gctggagcct tcttacgggc cgccggacgg cgccgatgat ccgcgggtcg
      841 ccaggatcgg ccggccgggg gagtacccgt tcactcgtgg cctctaccgc agcgggtatc
      901 gcggccgacc gtggacgatc cggcagttcg ccggcttcgg gaacgcgacg cagaccaacg
      961 agcggtaccg catgatcctg gacgccggcg gtggcggtct gtcggtcgct ttcgacatgc
     1021 ccaccctcat gggacgcgat tcggatgatc cgcaggctcg tggcgaggtc ggccattgcg
     1081 gcgtcgccat cgactcggcg tccgatatgg aggtgctgtt ccagggcatt ccgctcggcg
     1141 aggtcacgac gtcgatgacg atcagtgggc ccgcggtgcc ggtgttctgc atgtacgtcg
     1201 ccgccgccga acgtcaaggc gtcgcgcacg gcaagctgaa cggcacgctg cagaccgaca
     1261 tcttcaagga gtacatggcg caaaaagagt ggctgttcac gccgcagcca catcttcgcc
     1321 tcatcggcga cctgatggag ttctgcgcgc acgagatgcc ggactacaag ccgctgtcgg
     1381 tctctggcta ccacatcagg gaagccggtt cgacggctgt gcaagagctc gcctacacgc
     1441 tcgccaacgg cttcgcctac gtcgaacttg gtctgtcgcg acacatcgac gtcgacacgt
     1501 tcgcgccgca gttgtcgttc tttttcgatg cccacgttga cttcttcgaa gaaatcgcga
     1561 agttccgcgc ggctcggcgg atctgggcgc actggatgcg cgacgtctac ggcgcaaaga
     1621 cagaacgggc gcagtggttg cggtttcaca cccaaacggc aggcgtttca ctgacggctc
     1681 agcaaccgga caacaacgtc gttcggacgg cggttgaagc cctcgcggcg atcctcggcg
     1741 gcaccaactc gttgcacacc aatgccctgg acgaagtgct ggcgttgccg agcgagcgcg
     1801 cggcgtcgat cgcactgcgg acacagcaag tgctgctcga ggagaccggg ctgaccaacg
     1861 tcgtcgaccc gctcggcggc tcatggttcg tcgaggcgct taccgatcag atcgaggccg
     1921 aggcagagaa gatcttcacg cagatcagcg cgctcggcgg cgacggcagc atgatggccg
     1981 gagtgctgcg cggaatcgaa gacggctggt tcaccggcca tatcgcggag gctgcgtatc
     2041 gccagcagca ggagttcgaa tcgggggagc gccggatcgt cggcgtgaac tcctacaccg
     2101 acgacttcga accgccgctg gagatcatgc gcgtctcgca cgaggtcgag acagagcaat
     2161 gcgccaccct cgctgcccgt cgagcg
//
LOCUS       DATCBJ010000590         3497 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_203069, whole genome shotgun sequence.
ACCESSION   DATCBJ010000590 DATCBJ010000000
VERSION     DATCBJ010000590.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 3497)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3497)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3497
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_203069"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..639)
                     /locus_tag="VII50_11865"
     CDS             complement(<1..639)
                     /locus_tag="VII50_11865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5051590.1"
                     /translation="MTLTAGVLPLERRYRPSGPLDLDATLGPHRRGRFDPCHRVGPDG
                     AHWRTWRTPGGAATVRLAADPAAGEISTQAWGTGADWALDGVPSLLGADDDWTGLELP
                     PGNVRDARRRLPGMRLSSSRRVFEALVPAILEQLVTGIEARRTWTALVRRFGDPAPGP
                     APEGMAVLPSPAVIRSITDWEWHGVGLDGARRRTLHSAAAVASRLEEVGNVPA"
     gene            complement(636..1697)
                     /locus_tag="VII50_11870"
     CDS             complement(636..1697)
                     /locus_tag="VII50_11870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020662523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NDP-sugar synthase"
                     /protein_id="HEY5051591.1"
                     /translation="MLVGGKGTRLRPLTLTAPKPMLPTAGVPFLTHQLAQARAAGVDH
                     VVLATSYRAETFEQHFGDGSALGLDLEYVTETEPMGTGGGIRNVSERLRGDADSPVLV
                     FNGDVLSGHDIAAQLTLHAKHQAAVTLYLTEVADARAFGCVPVDSSGRVTAFLEKMPE
                     PVTNLINAGCYVFRRDVIDTIPDGQVVSVERATFPRLLASGATVMGYVDSGYWLDLGT
                     PAAFVQGSCDLVRGIAPSPAVPGPIGESLVLAGAQVAASAVVVSGSCIGAGAVVGENV
                     VVDGSVVFDDAVITAGAHVRASVIGRGARIGAGAVLDGVVIGDGADIGGGNELLTGVR
                     VWPGVVLAPGSVRFSSDES"
     gene            1873..>3497
                     /locus_tag="VII50_11875"
     CDS             1873..>3497
                     /locus_tag="VII50_11875"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HEY5051592.1"
                     /translation="MPRSKPPLAVWTATALHLGVMVIFTLFYPAYWGFDETGHVSRVL
                     DSQHGDLYPTPGVAAYVVGVNDTYQMFNLKDQPPFITYLPLPRANRPSFVSLGGGTLE
                     SEPTSVPNQLAQHPPGYYALAAGVLDAIPGSANLAWDQTVGLLRLLDVLFLLPLPYLW
                     WAIARRDTRSGVAVATAFAPILLPAFERLGGSVNNDDLLILLTSVFLLQVAKAMDGDT
                     RIRVAVTMGISLILALLTKGFALVLPPIAVLAYLVAWRRNRTPFPQWPVRIVVLAGAI
                     GGLWWLRNLNVYGRIQPAGLTPAQANVVWPPLPRGSTVHVATYAHHVLDRMTTDVWGA
                     LGLTVPPSLPGWITFVASVLVAALILLAFVATFAGRRVPAEQTTGRHAGAAQRVPVVD
                     LLVLLLPTVLVVVPLLFHGWSDYSRTGQLIGIQGRYLYPSFVGLVAIAAVGADLIVGL
                     LPSAVERFLPIVTCVLAIAIEVTAGCVVLSKLWLRKGSPLTGNLSRVAHAVGTNSPWP
                     GIVTIAMAAFVTLSALAALAVASAADPGTTHTS"
BASE COUNT          527 a         1268 c         1139 g          563 t
ORIGIN      
        1 ggcggggacg ttgccgacct cctcaagccg tgacgccacg gcggctgcgc tgtgcagggt
       61 ccgccgccgc gcaccgtcca ggccgacgcc atgccactcc caatcggtga tcgaacggat
      121 caccgcaggc gagggcagca ccgccatgcc ctcgggcgcc ggaccaggcg cagggtcgcc
      181 gaagcgacgc accaacgccg tccaggtccg ccgggcttcg atacccgtga ccagctgttc
      241 gaggatcgct ggcacgagtg cctcaaaaac ccggcgactg ctgctcaatc gcattccggg
      301 tagccgccga cgcgcgtcgc ggacgttgcc gggcggcaat tcgaggccag tccagtcgtc
      361 gtcggcaccg agcaacgaag ggacgccgtc gagcgcccaa tccgcgcccg tgccccacgc
      421 ctgcgtggag atctcgcctg cggcagggtc ggcggcgagc cggaccgtgg ccgcaccacc
      481 cggcgtccgc catgttcgcc agtgcgcacc atctggcccg acgcgatggc acgggtcgaa
      541 gcgaccgcgg cggtgcggac ccaacgtggc gtcgagatcg agcggcccac tcgggcggta
      601 gcgacgctcg agcggcagga ctccagcggt cagcgtcatg actcgtcgct cgagaaccgc
      661 accgagccgg gtgcgagaac cacgccgggc cacacccgca cacccgtgag cagttcgttg
      721 ccgccaccga tatcggcgcc gtcgccgatc accacgccgt cgagcaccgc acccgcaccg
      781 atgcgggcgc cacgcccgat cacgcttgcc ctcacgtgcg caccggccgt gatgaccgcg
      841 tcgtcgaaaa cgacgctgcc gtcgaccacg acgttctcgc cgaccaccgc ccccgcaccg
      901 atgcagctgc cgctgacgac gaccgccgag gcggcgacct gcgcgccggc aagaaccagc
      961 gactcaccga tcggtcccgg tactgcaggt gaaggcgcga tgccacgcac caggtcgcac
     1021 gacccttgca cgaacgcggc cggcgttccg agatcgagcc agtagcccga atcgacgtac
     1081 cccataaccg tcgcaccact cgccagcagc cgcgggaatg tcgcgcgttc gacggaaacc
     1141 acttgcccgt caggaattgt gtcgatgacg tcgcgccgga agacgtagca accggcgtta
     1201 atgaggttcg tcacgggctc gggcatcttc tccaggaacg cagtcacccg cccggaggaa
     1261 tcgaccggca cgcagccgaa cgcccgcgca tcggcgacct cggtgaggta cagggtgacc
     1321 gccgcctggt gcttggcgtg cagggtgagc tgcgcggcga tgtcgtggcc cgagagcacg
     1381 tcgccgttga acacgagcac cggcgaatcg gcgtccccgc gaagccgctc gctgacgttg
     1441 cggatcccgc cgccggtgcc catcggctcg gtctctgtca cgtactccag atcaagcccg
     1501 agcgccgaac cgtcgccgaa gtgctgttcg aaggtctccg cgcggtacga ggtggcgagc
     1561 accacgtgat cgacgccagc ggcacgggct tgggcgagct gatgggtcag gaacgggacg
     1621 ccggcagtcg gcagcatcgg cttgggcgct gtgagggtga gcgggcgcag ccgggtgccc
     1681 ttgccgccga ccagcacgat cgcgtcgatg acgcactccc ctgtcttgcc gtcacacccc
     1741 tgctgcgcag cttaggcgag ccgcttagcg acatcggcga ggcgtatacg gcgagccgct
     1801 catctggttc ccagcttcgc gggctagcgt tcgggtcgga cgacgggggc gcgcccttct
     1861 ggaatcgagc ccatgccgcg ctccaagccc ccgctcgccg tgtggacagc tacggcgctg
     1921 cacctcggcg tcatggtcat cttcacgctc ttctaccccg cgtactgggg gttcgacgag
     1981 accggccatg tctcgcgggt gctcgactcc cagcatggcg acctctaccc gaccccgggc
     2041 gttgccgcgt acgtcgtcgg agtcaacgac acataccaga tgttcaatct gaaggaccag
     2101 cccccgttca tcacctacct gccgctcccg cgcgcgaacc ggccgtcttt cgtcagcctg
     2161 ggcggcggca ccttggagtc ggaacctacc tccgtgccga accagctggc ccaacaccca
     2221 ccgggctact acgccctggc ggccggcgtg ctcgacgcca taccgggctc cgcgaacctg
     2281 gcgtgggacc agacggtcgg gctgttgcgc ctgctcgacg tgctcttcct gttgccgctg
     2341 ccgtatctgt ggtgggcgat cgcgcgacgc gacacccgta gcggcgtcgc cgtggcgacc
     2401 gcgttcgcac cgatcctgct accggcgttc gaacgccttg gcgggtcggt caacaacgac
     2461 gacctgctga tcctgctgac aagcgtcttc ctgctgcagg tggcgaaggc gatggacggc
     2521 gacacccgca tccgcgtcgc tgtgacgatg ggcatcagct tgattcttgc cctgctcacc
     2581 aagggcttcg cgctcgtctt gccgccgatc gccgtgctgg cctatctcgt cgcgtggcgg
     2641 cgcaaccgca cgccattccc gcagtggccg gtccgcatag tggtcctggc cggcgcgatt
     2701 ggcgggctgt ggtggcttcg caacctcaac gtgtacggcc gcattcaacc ggccggactc
     2761 actccggcgc aggccaacgt cgtgtggccc ccattgcctc gcggctcgac ggtgcacgtg
     2821 gcaacgtacg cccaccacgt gctcgatcgg atgacgaccg acgtctgggg cgcgttgggc
     2881 ctgacggtgc caccgagcct gcccggttgg atcacgttcg tcgccagcgt gctcgtcgcc
     2941 gcgttgatcc tgctggcctt cgtcgccacc tttgcgggac ggcgggtacc tgccgaacaa
     3001 accaccggcc ggcacgccgg ggccgcccaa cgtgtgcccg tcgtcgacct gctcgtcttg
     3061 ctgctgccaa cggtgctggt cgtcgtgccc ctgctgttcc acggctggtc cgactactcg
     3121 cgcaccgggc aactcatcgg catccagggc cgctatctct acccgagttt cgtcgggctg
     3181 gtcgcgatcg cggcggtcgg cgccgacttg atcgtcggcc tcctgccatc ggcggtcgaa
     3241 cgcttcctac cgatcgtgac gtgcgtgctg gccatcgcga tcgaagtgac tgcgggctgc
     3301 gtcgtcttgt cgaagctgtg gctgcgcaag ggatccccgc tcaccgggaa tctctcgcgg
     3361 gtcgcgcacg ccgtcgggac caactcgccg tggcccggca tcgtcacgat cgcgatggcg
     3421 gcgttcgtga ctcttagtgc actggctgct cttgcggttg cgtcggccgc tgatccaggt
     3481 accactcata cgtcgag
//
LOCUS       DATCBJ010000591         4264 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_164672, whole genome shotgun sequence.
ACCESSION   DATCBJ010000591 DATCBJ010000000
VERSION     DATCBJ010000591.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 4264)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4264)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4264
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_164672"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..592)
                     /locus_tag="VII50_11880"
     CDS             complement(<1..592)
                     /locus_tag="VII50_11880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEY5051593.1"
                     /translation="MVSGSGASEFDYPLDEEHEALRSTVADFARQVVAPRAAAMDEAG
                     AFPYDIVAAMARMGLFGLPFPQEYGGMGGDYFALCLAIEELARVDSSVAITLEAAVSL
                     GAMPVFRFGTDEQRKEWLPQLTCGEALAAFGLTEPDGGTDAGATRTRATLADGVWRID
                     GTKAFITNAGTDITRLITVTAVTGEAGSRKEISSLLV"
     gene            743..1231
                     /locus_tag="VII50_11885"
     CDS             743..1231
                     /locus_tag="VII50_11885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HEY5051594.1"
                     /translation="MTVVRSLYARFAKLGHEVAKFGTVGLIAYVIDTGTFNLLKYDGG
                     PVSHKVLTDKIISTALAATFAYFANRHWTWRARARTGLGREYLLFFVFNGIGLGIALA
                     CLATSHYVLDLRSGLADNISANVIGTVLGTLFRFWSYRRWVFIEAEEPLRTEEAASAA
                     VV"
     gene            complement(1228..2517)
                     /locus_tag="VII50_11890"
     CDS             complement(1228..2517)
                     /locus_tag="VII50_11890"
                     /inference="COORDINATES: protein motif:HMM:NF037721.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY5051595.1"
                     /translation="MRRRLLVSTLVVAVVSVILLGIPLGIVTTKLIRDEAQKRINREA
                     TQIVAALDDSASTSPSDTSLDASATAATMAILKQFAGSDRYVSVVQADQTLTTGTPIT
                     GSLDRGTAIGTKDETVTVVASASSTNSELASVWVGVAALAIVSLTAAVELARRQAKRL
                     GGPLEDLAVGAERLGAGDPRPRNHRYGVPELDKVAEVLDRGAERIGRLLGAERELAAD
                     ASHQLRSPLTALSIRLEEIVATSTEPAVREEATAALVQAERLAAVVDSLLAEHSRSAR
                     SESGVVVAIDAVLAQQVVEWDPAFRRASRRLVRDGSTGLTAMSTPGGLAQVVATLIDN
                     ALVHGAGTVTVHTRETSGHVVLEVRDEGPGVADELVPHIFERSFSGAHRTGLGLALAR
                     DLVAADGGRLELAQPRPPIFAIFLAPDNADGTGPETP"
     gene            complement(2524..3192)
                     /locus_tag="VII50_11895"
     CDS             complement(2524..3192)
                     /locus_tag="VII50_11895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020128522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEY5051596.1"
                     /translation="MTAVLLAEDDVSISDPLARALRREGYEVEVRSEGTAVLQRVLSG
                     GVDLVVLDLGLPTMDGLEVCRRMRAEGRSVPVLVLTARAEEVDLVVGLDAGADDYVTK
                     PFRLAELLARVRALLRRGAPEVPDVNGVRINAESRRAWLGDEELSLTAKEFDLLLALV
                     LGAGKVLTREQLMRDVWGMSRGASSKTLDMHVSWLRRKLGDDAASPRLITTIRGLGFR
                     FERG"
     gene            complement(3217..>4264)
                     /locus_tag="VII50_11900"
     CDS             complement(3217..>4264)
                     /locus_tag="VII50_11900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HEY5051597.1"
                     /translation="PSAAGGAYVPAFCDIVIMVEGNASMYLGSDRMVEMVTGEKTTLE
                     AMGGARMHCTESGVGHVLVQSENEALATVRRYLSFLPSNWQGEPPNTPARAAEEIDLR
                     ALVPASERVAFDMRRFIRGLVDVGSLFEIHALWAREVVVGLARLGGKPVGVVANNSLH
                     KGGVLFVDSADKATRFIQLCDAFNVPLIFLADVPGFMVGTAVEKAGIIRHGAKMVSAV
                     SEATVPKFCVVVRKAYGAGLYAMAGPGFEPDATLALPTAKIAVMGADAAVNAVHANRL
                     AAIADDEERAAVVAGLRAEYEADIDIMRLAAELVVDDIVEPEDLRANLIRRLGAAATK
                     ERVFSRRRHGITPV"
BASE COUNT          704 a         1518 c         1377 g          665 t
ORIGIN      
        1 gcaccagcag cgacgagatc tccttgcgac tgccggcttc gccggtcacg gcggtcacgg
       61 tgatcaggcg ggtgatgtcg gtgccggcgt tggtgatgaa cgccttcgtc ccgtcgatgc
      121 gccagacgcc gtcggcaagc gtggctcggg tgcgggtcgc ccccgcgtcg gttccgccgt
      181 ccggctcggt cagcccgaaa gccgccagcg cctcgccgca agtgagctgc ggcagccact
      241 ccttgcgctg ctcgtcggtg ccaaaccgga agaccggcat ggcgccgagc gacacggccg
      301 cctccagggt gatcgccacc gacgagtcga cccgggcaag ctcctcgatg gccaggcaga
      361 gcgcgaagta gtcgccgccc ataccgccgt actcctgcgg gaacggcagc ccgaacaggc
      421 ccatccgggc catcgccgcg acgatgtcgt aggggaacgc gcctgcctcg tccatcgcgg
      481 ctgcccgcgg ggctacgacc tggcgagcaa aatcggccac cgtggaacgc aacgcctcgt
      541 gctcttcgtc caagggatag tcgaactcgg aggcaccgct gccactcacc acgggtccat
      601 cctttcccgg ccggcacgtt acagccggcc ggccagctcg tgtcgcgagc gtcgggaggg
      661 ggcactcccc tcagcatgtt cccattttcg gccgatacgc tgaccaaggc gccaaatcgt
      721 gcgtcccaat cgaggtggtg acgtgacggt cgtgcggtcg ctttacgcgc gcttcgcgaa
      781 actcggccac gaggtcgcca agttcggcac cgtcggcctg atcgcctacg tcatcgacac
      841 cggcacgttc aacctgctga agtacgacgg tggcccggtc tcgcacaagg ttctgaccga
      901 caagatcatc tcgaccgccc tggccgcgac cttcgcctac ttcgcgaatc ggcactggac
      961 ctggcgggca cgcgcgcgga ccggcctcgg ccgcgagtac ctgctgttct tcgtgttcaa
     1021 cggcatcggg ctcggtatcg cgctcgcctg cctcgcgacg tcccactacg ttctcgatct
     1081 tcgcagtggc ctcgccgaca acatctcggc gaacgtcatc ggcacggtcc ttggaacgct
     1141 cttccggttc tggtcgtatc ggcgctgggt gttcatcgag gccgaggagc cgttgcgcac
     1201 cgaagaggca gcatccgccg cagtcgtcta gggcgtctcg ggcccagtgc cgtcagcgtt
     1261 gtcgggcgcg agaaagatcg cgaagatcgg cggccgcggc tgggccaact ccagccgccc
     1321 gccgtccgct gctaccaagt cgcgtgcaag cgcgagccca agtccagtgc ggtgcgcacc
     1381 gctaaacgat cgctcgaaga tgtgcggcac gagttcgtcg gccacgccag gcccctcgtc
     1441 gcgaacctcg agcacgacat gtccgctcgt ctcgcgggta tggaccgtca ccgtgccggc
     1501 gccatgaacg agggcgttgt cgatcagcgt cgccacgacc tgcgcgagtc cgccgggtgt
     1561 tgacatcgcg gtcaggccgg tactgccgtc gcgcacgagg cgccggctag cccgccgaaa
     1621 cgcggggtcc cactccacca cttgctgagc cagcaccgcg tcgatggcga ccacgacgcc
     1681 ggactccgag cgcgcgctgc ggctgtgctc ggccagcagc gagtcgacca cggcggccag
     1741 ccgttcggcc tgcaccagag ccgccgtggc ctcctcgcgc accgccggtt ctgtcgacgt
     1801 cgcgacgatc tcctcgagcc ggatcgacaa cgcggtgagc gggctgcgca gctgatgcga
     1861 tgcgtcggcg gccaactcgc gctcggcgcc caagagccgg ccgatgcgtt cggcaccgcg
     1921 gtcgagaacc tccgccactt tgtcgagttc gggcacgccg taccggtggt tgcggggtcg
     1981 cgggtcaccc gcaccgagcc gctcggcgcc gaccgcaagg tcctcgagag gtccaccgag
     2041 ccgctttgcc tgccggcggg cgagctcgac ggctgccgtg agcgacacga tcgccagcgc
     2101 cgcgacaccg acccacacgg acgccagctc gctgttcgtc gacgacgccg aggcgacgac
     2161 ggtcacggtc tcgtccttcg ttccgatcgc ggtgccccgg tcgagcgatc ctgtgatcgg
     2221 cgtcccggtg gtcagcgttt gatccgcttg gacgacggag acgtagcggt cggacccggc
     2281 gaattgcttc aggatggcca tggtcgccgc ggtcgccgat gcatcaagcg atgtatcgga
     2341 cggcgaggtg ctggcgctgt cgtccaacgc tgcgacgatc tgggtcgctt ctcggttgat
     2401 ccgcttctgt gcttcgtcgc ggatcaactt cgtcgtaaca atgccgagcg gaattcccag
     2461 caggatcacc gaaacgacgg cgacgaccag cgtggagacc agcagtcgcc ttcgcaccgt
     2521 ggatcaacca cgctcgaagc ggaaccccaa gccacggatc gtcgtgatga gccggggtga
     2581 cgcagcgtca tcgccgagct tgcgacgcag ccacgacaca tgcatgtcga gagtcttcga
     2641 ggacgccccg cggctcatgc cccagacgtc gcgcatcaac tgctcgcggg tgagtacctt
     2701 gccggcgccg agtacaaggg cgagcagcag gtcgaactcc ttcgccgtca agctgagttc
     2761 ctcgtcgccc aaccacgcgc gccgcgattc cgcgttgatg cgcacgccgt tgacatcggg
     2821 cacctcgggg gcaccgcgcc gcaacagcgc ccgcacccga gccagcagct cggcaagccg
     2881 gaacggcttg gtcacgtaat cgtcagcgcc cgcgtcaagg ccgacgacga ggtccacctc
     2941 ctcggcccgg gcggtcagca cgagcaccgg caccgagcgg ccttcggcgc gcatccggcg
     3001 gcatacctcg agaccgtcca tcgtgggcag cccgagatcg agcaccacga ggtcgacccc
     3061 gccgctgaga acacgctgca gcaccgcggt gccttcactc ctgacctcga cctcgtaacc
     3121 ctcacggcgc aaggcccggg cgagcgggtc ggaaatcgag acatcatcct cggcgagcaa
     3181 cacggcagtc atgaaggaat cgtatgggtc ggcgagctaa accggggtaa tcccgtggcg
     3241 ccttcgcgaa aacacccgct ccttggtcgc ggcggcgccc agccggcgga tcaagttggc
     3301 ccgcagatcc tcaggctcaa cgatgtcgtc gaccaccaat tcggcagcca gccgcatgat
     3361 gtcgatgtcg gcttcgtact cagcgcgcag cccagcgacc accgcggccc gctcttcgtc
     3421 gtcggcgatc gcggcgagcc ggttggcgtg cacggcgttg accgctgcgt cggctcccat
     3481 caccgcgatc ttggccgtcg gcagcgcaag agtggcgtcc ggctcgaacc ccgggccggc
     3541 catcgcgtag agccccgcgc cgtaggcctt gcgcacgacc acgcaaaact tcggcacggt
     3601 tgcctccgac acagcgctca ccatcttggc gccgtgccgg atgatgccgg ccttctcgac
     3661 agcggtaccg accatgaatc ccggcacatc tgccaggaag atcaacggca cgttgaacgc
     3721 gtcgcacagt tggatgaagc gggtcgcctt atctgcggag tcgacgaaca gcacgccgcc
     3781 cttgtgcagc gagttgttgg cgacgacgcc cacgggcttg cccccgaggc gggccagccc
     3841 gaccacaact tcacgcgccc acagcgcgtg gatctcaaac aggctcccga cgtcgaccag
     3901 accgcgaatg aagcgacgca tgtcaaaagc gactcgctcg ctggccggga caagcgcgcg
     3961 aaggtcgatc tcctcggcgg cgcgcgctgg cgtattcggc ggctcgccct gccagttcga
     4021 cggcaagaac gacagatagc ggcgcacggt cgccagcgcc tcgttctcgc tttggacgag
     4081 aacgtggccg acgcctgatt ccgtgcagtg catgcgagcg ccacccatgg cttcgagcgt
     4141 ggtcttctcg ccggtcacca tctcgaccat gcggtcggag ccgaggtaca tcgaggcgtt
     4201 gccctcgacc atgatgacga tgtcgcagaa cgccggcacg taggcgccgc cggccgcgct
     4261 cgga
//
LOCUS       DATCBJ010000592         3304 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_248772, whole genome shotgun sequence.
ACCESSION   DATCBJ010000592 DATCBJ010000000
VERSION     DATCBJ010000592.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 3304)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3304)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3304
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_248772"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..385
                     /locus_tag="VII50_11905"
     CDS             <1..385
                     /locus_tag="VII50_11905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5051598.1"
                     /translation="DPPASSSANPLSSNLGKCPTVLPLIPGSDAAAQAKAAAVKVVPT
                     IYPPEPTKDFTVTAMYPASQSLDGGKGIGTIAYGTCGKTIGDRTWVVEVHFPHDPNNS
                     ASTGNGQLFVSEFANGGWQVWFRNH"
     gene            complement(392..1456)
                     /locus_tag="VII50_11910"
     CDS             complement(392..1456)
                     /locus_tag="VII50_11910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-eliminating lyase-related protein"
                     /protein_id="HEY5051599.1"
                     /translation="MADDLNGQHHDPARRGFASDNYAGVHPEILGALAVANGGHVGSY
                     GADPYTSAAVEVLQGHFGADAEVFFVFNGTGANVVSLQAMTQRWDAVICAESAHINID
                     ECGAAEKIAGLKLLSVATPEGKLTPELIDVHAWGWGDVHHAQPRVVSITQSTELGTVY
                     TPSEVAAICAHAHSLGMFVHVDGARIANAAAALGVPLRAFTTDVGVDVVSLGGTKNGL
                     LYGEAIVVLNRDAASGVEFLRKAGMQLASKMRFVGVQFEVLFGTDLWLRSATHANAMA
                     RRLADAVADVPGVQLSREPAVNSVYAILPAESTARLQEQYFFYPWDRTTGEVRWVCAF
                     DTTEQDVDDFAEAIRKEMAR"
     gene            complement(1441..2148)
                     /locus_tag="VII50_11915"
     CDS             complement(1441..2148)
                     /locus_tag="VII50_11915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEY5051600.1"
                     /translation="MSLDGAVVVVAGAGGGAGAAVVRRLASAGAIVVSADRSLDSTES
                     LVREIGEDGGQIDAYALDLLDEDTTRRWADSVTSRFGRVDGVVHLVGGWRGGKGITEA
                     DLADWGWLSGLLVRTVQNTTRGFHDQLKASPIGRFVLVSAAEASRPTAKNASYAAAKA
                     AAEAWTLAVADSFRDSDAAATIVVVKALVTPQMREAKPDAAFKGYTDVVELADTIVGL
                     WDKPAQEVNGERVWLTT"
     gene            complement(2145..>3304)
                     /locus_tag="VII50_11920"
     CDS             complement(2145..>3304)
                     /locus_tag="VII50_11920"
                     /inference="COORDINATES: protein motif:HMM:NF039440.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF6421 family protein"
                     /protein_id="HEY5051601.1"
                     /translation="HFEHDADYLDAVILDLQRWRDGGFGVPDFLDSLMAFHPELHRVD
                     GLEHLVVFPMYTQNGNPDRNLEAVVIRVVWPDFVAALEATRYDNSMFVPISFVDFTAG
                     YDTNSAVLFPETVAVREVPRYTWGAIFCDREAARFRRVSAAAADTLKLALPPAAERLI
                     DNQALAQNTFVLWDLIHDRTHSHGDLPFDPFMIKQRMPYWMYSLEELRCDLQAFAQAV
                     DLEAEHVTPYGDLVQYAVLFDRLFRFPITGDRVRNYDGLGGQLLFAYLHKNGVLRWTD
                     NTLSIEWSRIASAVTGLGGEVETLYRDGIDRSRVGHWLAGHEFVARYVAPSPVSVWAR
                     GAAALPLDGPPKGLVDLVLPDEFPLNVFYEALRRKLGDVIESTRGITAAAS"
BASE COUNT          592 a         1116 c         1062 g          534 t
ORIGIN      
        1 cgacccgccg gcgtcatcga gtgcgaatcc gttgtcaagc aacctcggca agtgcccgac
       61 ggtgctaccg ctgataccag gatctgacgc agctgcgcag gcaaaggcgg ccgcggtcaa
      121 ggtggtgccg acgatctatc cgcccgagcc gacgaaggac ttcaccgtca ccgcaatgta
      181 tccggccagc cagagcctcg acggcggcaa gggtatcggg acgatcgcgt acggcacgtg
      241 tggcaagacg atcggcgacc gcacatgggt agtcgaagtc cacttcccac acgatccgaa
      301 taacagcgcg tcgaccggca acgggcagct attcgtctcg gagttcgcga acggcggctg
      361 gcaggtctgg ttccgcaacc actgagctcg gtcagcgtgc catttctttg cggatcgcct
      421 cggcgaagtc gtcgacatct tgctcggtgg tgtcgaacgc gcacacccag cgcacctcac
      481 cggtcgtgcg gtcccacggg tagaagaagt actgctcctg caatcgtgct gtcgactccg
      541 ccggcaaaat cgcgtacacc gagttgactg ccggttcgcg gctgagctgc acgcccggca
      601 cgtcggcaac ggcgtccgcc aaccgccgtg ccatcgcgtt cgcgtgtgtc gcgctgcgca
      661 gccacaggtc ggtgccgaat aacacctcga attgcacgcc gacgaatcgc atcttcgacg
      721 ccaactgcat gccggccttg cgcaggaact ccaccccgct cgcggcgtcc cgattgagaa
      781 cgacgatggc ctcgccgtac agaagaccgt tcttcgtccc accgagactg acgacatcga
      841 cgccgacgtc ggtcgtgaat gcacgtagcg ggacgccgag cgcggccgcc gcgttcgcga
      901 tgcgcgcgcc atcgacgtgc acgaacatcc ccaacgaatg ggcgtgcgca cagatcgcgg
      961 cgacctccga gggtgtgtac accgtaccga gctcggtgct ctgggtgatc gacacgacgc
     1021 gcggctgcgc gtggtgcacg tcaccccaac cccaagcgtg gacgtcgatc agctcgggtg
     1081 tgagcttccc ctcgggagtc gccaccgaga gaagcttgag cccggcaatc ttctccgccg
     1141 caccacactc gtcgatgttg atgtgcgccg attccgcgca gatcacggca tcccagcgct
     1201 gcgtcattgc ttgcaatgac acgacatttg cgccggtgcc gttgaagacg aagaacacct
     1261 cggcgtccgc accgaagtgt ccttgcagta cctcaacagc ggcggacgtg taagggtcgg
     1321 cgccataact gccgacgtgc ccgccgttgg cgaccgctag cgcgccgagg atctcgggat
     1381 gtactcccgc gtaattgtcg ctggcgaatc cgcggcgggc cgggtcgtgg tgctgcccgt
     1441 tcaggtcgtc agccataccc gctccccatt cacttcttga gctggcttgt cccacagacc
     1501 aacgatggtg tcggccagct cgacgacatc ggtgtagccc ttgaacgccg cgtcgggctt
     1561 ggcctcacgc atctgcggtg tgaccagtgc cttgaccacg acgatggtgg ctgcggcgtc
     1621 ggaatcgcgg aatgaatcgg cgacagcgag cgtccacgcc tctgcggcgg cttttgctgc
     1681 ggcatacgag gcgtttttcg cagtcggccg cgacgcttca gctgccgaca ccaacacgaa
     1741 ccggccgatc gggctggcct tcaactgatc gtggaatccg cgcgtcgtgt tctgcaccgt
     1801 gcgcacgagc aagcctgaca accagcccca atcggcgagg tcggcctcgg tgatcccttt
     1861 gccgccacgc cacccgccga cgaggtgcac aacgccgtcg acgcgaccga atctcgacgt
     1921 gacggagtcg gcccacctgc gcgtcgtgtc ttcgtcgagc aggtcgaggg cgtaggcgtc
     1981 gatctggccg ccatcctcgc cgatctcgcg gaccagcgac tcggtcgaat cgagtgaccg
     2041 atcggcgctc acgacgatcg cgccagccga agccaaccgg cggacgacgg cagctcccgc
     2101 cccaccgccg gctcccgcga caaccacgac ggccccgtcg aggctcatga ggcggccgct
     2161 gtgatgccgc gcgtcgactc gatgacgtca ccgagcttgc gacgcaacgc ctcgtagaac
     2221 acgttcagcg ggaactcgtc gggcagcacc aggtcgacga gtcccttcgg cggcccgtcg
     2281 agtggcaagg cagccgcacc gcgagcccac accgataccg ggctcggcgc tacgtaccgt
     2341 gcgacgaact cgtggccggc cagccagtga ccgacccgcg agcggtcgat gccgtcgcgg
     2401 tacagcgtct cgacctcgcc accgagaccg gtgacggcgg acgcgatgcg cgaccattcg
     2461 atcgacagcg tgttgtcggt ccagcgcagg acgccgttct tgtgcaggta ggcgaacagc
     2521 aactgaccac cgaggccgtc gtagttgcgc acgcggtcgc cggtgatggg gaaccgaaac
     2581 agccggtcga acagcactgc gtactgcacg aggtcgccgt agggcgtgac gtgctcggcc
     2641 tcgagatcga ccgcctgcgc gaacgcctgc aggtcgcagc gcaactcctc gagtgagtac
     2701 atccagtacg gcatccgctg cttgatcatg aacgggtcga acggcaggtc gccgtggctg
     2761 tgcgtgcggt cgtgaatcag gtcccacagc acgaacgtgt tctgcgcgag cgcctggttg
     2821 tcgatgaggc gctcggcggc cggcggcagc gcgagcttca acgtgtcggc cgccgccgcg
     2881 ctgacgcgcc ggaagcgggc cgcctcacga tcgcagaaaa tcgcgcccca ggtatatcgc
     2941 ggcacttcgc gaaccgccac cgtttcgggg aacagcaccg cggagttcgt gtcgtagccg
     3001 gccgtgaagt cgacgaacga gatcggcacg aacatcgagt tgtcgtagcg cgttgcctcc
     3061 agcgccgcga cgaagtcggg ccacacgacg cggatcacga cagcctccaa gttgcggtcg
     3121 gggttgccgt tctgggtgta catggggaac accacgaggt gctcgagccc gtcgacccga
     3181 tgcagttcgg ggtggaacgc catcagcgaa tcgaggaagt cgggcacccc gaatccgccg
     3241 tcgcgccagc gctggagatc gagaatgacg gcgtccagat agtccgcgtc gtgttcgaag
     3301 tgcg
//
LOCUS       DATCBJ010000593         7359 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_54238, whole genome shotgun sequence.
ACCESSION   DATCBJ010000593 DATCBJ010000000
VERSION     DATCBJ010000593.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 7359)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7359)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7359
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_54238"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..401)
                     /locus_tag="VII50_11925"
     CDS             complement(<1..401)
                     /locus_tag="VII50_11925"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEY5051602.1"
                     /translation="MRILVASDLHYRLPHYDWLVRAAEDVDVVALAGDLADVVNAVPF
                     DVQVVVLERYLVTMAQRATVLAASGNHDLDGPGDLGEQVASWLRDLRHETLRCDGASV
                     DLDGIRFTVCPWWDGPATRDALAAQLAAAAV"
     gene            544..5466
                     /locus_tag="VII50_11930"
     CDS             544..5466
                     /locus_tag="VII50_11930"
                     /inference="COORDINATES: protein motif:HMM:NF012271.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HEY5051603.1"
                     /translation="MRGAMRPVVIGLATVTFFATTAAGAYAASAPAATAACTAINAPP
                     GMALLPASDDCPTPGARTTLVKAAAVPPTTPISETALTYDHTNGDLLAITKVDGTNDL
                     AIGGNFSLLYTEDGVSHTAINFAVIDEATGTFLYGGSAGGSTDKYVRSIGSLNGNIYV
                     GGDFTSWAGVSRLHAVMLAPTGNPASAYAVTSWNPAPSIRVRGLAVDSSAVYLGIESS
                     IRAVNPTTGATIWSKGVSSGSVASVFKYQGFLFVGGLFNSYNGVAHPGLVKVNPSDGS
                     LVTAFDAHLRPNTGQGQYGAYDGEEIITMAPGPNAGEILAGSGGHAPVGDSSNEALLL
                     DVNTGARLMHYSTLGDCQGIGTVGDTTVAGYHNNTASASNPLSANYFGIQLENSGGAN
                     SLGVPTTWDPRVNGNQGNADGGNNGIQAMYVDQGTQTIYLGGAFLHWNGTTGSNHQSL
                     IAFSFAPSNPTAPGSPTGVTATAGDTTATVSWTAPSDGGSPITGYTVTSDPGAIVATT
                     TGATSVVVPGLTDGVTYTFTVTATNAINTSGSSAPSNPVTPTAGTLQSNSAEGGTPGA
                     SVTTANSGGASGTAFTIVSKGSGATITYAAAAAVHGALGYALTGSSGTATLMGWNGYS
                     ATSMAIRFYYNPGSTLPNQVLRLADIRNSTATAARVELSASNQIFIQNNAGTTVTTFA
                     HALQANTWYRIEMAISVSSSAASINAAYYTLDSTTPVDPAYTTATGNTGTASITQVTV
                     GSTASATWTGTSYFDDIASQSPSTAFIGPVSASPTAPDAPTAVSAVPGDGQATVSWTA
                     PLSNGGSAITGYTVTSSPGGFTATTAGPTSAIVSGLTDGTPYTFTVTATNGVGTGPAS
                     SPSSSVIPVAAPTAPGAPTGVNATPGDGQATVSWTAPLSNGGSAITGYTVTSSPGGFT
                     GTSAGAATTAVVTGLADGTSYTFTVTAANSVGTGAASTPSPAVMPVAAPTAPGAPTAV
                     SAVPGDGQATVSWTAPSSNGGAAITGYTVTSSPGGVTVSGPASPATVTGLTDGVSYTF
                     TVTAANSVGTGPASAPSTGVIPAAAATAPGAPTAVSAVPGDRQATVSWTAPLSNGGSA
                     ITGYTVTSTPGSFTATTAGATSAAVTGLSDGISYTFTVTAANTVGTGPSSSPSPAVTP
                     ATTPGAPTGVTGTPGETQATVTWTAPVSNGGSAITGYTVTASPGVATATTAGATSAVV
                     IGLTDGTPYTFTVTATNGNGAGPASDPSPSVVPGPTVPDAPTGVSAVPGDTQATVSWT
                     APASNGSPITNYTVLSSPGGFMATGATSPLTVTGLTDGTSYTFTVTATNGVGTGAASL
                     PSSSVIPTAAATAPGAPTGVTATPGNTQATVTWTAPSNGGSAITGYTVTSSPGGFTAN
                     SAGPTSALVSGLTNGTTYTFTVTAANGVGTGPASGSSNTVTPSAGTFVSNSAEGGISG
                     TSVTTGNSGGASGNALTVVTKGAGAALVYSTAAAAHGSLGYALTGASGTATLLGWNGY
                     TAPSIAVRFYYNPGTTLPNAVLRLADIRNSTATAARVELSASNQLFIQNAAGATVTTF
                     PHALQANTWYRVEITISVSASTATIQAAYYLSSSTAPVDPAYSTVTGNTGTTNITQVS
                     IGSAASATWVGTSYFDDLAVQSLSTAFIGP"
     gene            complement(5500..5976)
                     /locus_tag="VII50_11935"
     CDS             complement(5500..5976)
                     /locus_tag="VII50_11935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5051604.1"
                     /translation="MTEMLSTRDMTVRSVIGSRGQAFALERPVPVRAGQRVYYGIRVD
                     GRPLVWVASDTPIFDRVEIDQLNPDADVIGVSAAEDVPRPMQNRFIGVMADGDPYVNG
                     ATGGWSSNGQRDYRLVDAIADVADLIYRALESNGRQIDLTRLPSNNNLLIPLPRGI"
     gene            complement(6105..>7359)
                     /locus_tag="VII50_11940"
     CDS             complement(6105..>7359)
                     /locus_tag="VII50_11940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5051605.1"
                     /translation="ISAYDMAVSDAVYGKSGRYVSMPRLQAMLDLEFGLNLERLDAER
                     GDSNAFFAFADTVTARSFSGGNECHGWMGVKFQAHPHDEPSQIIMHVRMLDVEAALQQ
                     EALGIVGVNLLHAAFFQHHEPEMLVESLLDRLTTGRIEIDMIELKGIEFRSVDNRLMA
                     LKLVQVGLSGAAMFGPDREVLQPSEVLRKKAILVERGSFRPPTVVNIDMLESALAKFI
                     SDPDVASREILMLTELTMANLLAGGGDVDRRDFLARADLLAACGMTVLISDYVEYHRL
                     AAYLAWRTDGHIGMVMGIPSLMDLFNESNHAALPGGILESFGRLFKNDLKLFVYPMLR
                     DERITTVDDLSVGEDLQPLYNYLARRGSFVGLDNYKPDYLPILSRDVLRRIASGDVTW
                     ETMVPPEVGELIKKRGFFGYHRTDE"
BASE COUNT         1272 a         2497 c         2352 g         1238 t
ORIGIN      
        1 tcgacagcgg ccgcagccag ctgtgcggca agagcgtcac gggttgcggg accgtcccac
       61 cacgggcaca cagtgaagcg aatcccatcg aggtcgacgg aggcgccgtc acaccggagc
      121 gtctcgtgtc gcagatcgcg cagccagctc gccacctgct cgcccaggtc gccaggcccg
      181 tcgaggtcgt ggttgcccga ggcggcaaga acggtggcgc gctgcgccat cgtgacgagg
      241 tagcgctcga gcacgaccac ctgcacatcg aacggcaccg cattgacaac gtcggcaagg
      301 tccccggcca gcgccacgac gtcgacgtcc tcggcggccc ggaccaacca gtcgtaatgc
      361 ggcagtcggt agtggaggtc ggaggccacc aagatgcgca cgcgctgatc ttgccttact
      421 tgggctttgc cagcgattga agaactagga ggacaaactc gcgcgcgatt tgtgccccag
      481 cgctatgtga ttgaggtcac gtgagccaat agcatgtcca gcaattcctg aggggggaaa
      541 tccatgcgcg gagccatgcg ccctgttgtg atcgggcttg ccacagtcac gtttttcgcg
      601 actactgctg ccggtgccta cgccgcttcc gctcccgctg cgacggctgc ctgcacggcg
      661 atcaacgcgc cgccggggat ggcgctactg ccggcctccg acgactgccc gacgccgggc
      721 gcccgcacca ccctggtcaa ggcggcggcg gtgcccccga ccacaccgat ctccgagacg
      781 gcgttgacct acgaccacac caatggtgac ctccttgcca taaccaaggt cgacggaacc
      841 aacgatctcg cgatcggcgg caacttcagc ctcctctaca cggaggacgg cgtttcgcac
      901 accgcaatca actttgcggt cattgacgaa gcgacaggca cgtttctgta cggcgggagt
      961 gcaggcggca gcaccgacaa atacgtgcgg tcgatcggca gcctcaatgg gaacatctac
     1021 gttggcggcg actttacttc gtgggcaggt gtatcccgac tgcacgcggt catgctcgcg
     1081 cccaccggca accccgcatc ggcgtatgcg gtgacttcgt ggaatcctgc gcccagcatc
     1141 agggtgcgtg gcttggcggt cgacagcagc gccgtctacc tcggtatcga gagctcgata
     1201 cgtgcggtga acccaacgac tggggcgacc atctggtcga agggcgtctc gagcggctcc
     1261 gttgccagcg tctttaaata ccagggcttc ctcttcgtcg gtggcttgtt taacagctac
     1321 aacggggttg cgcatcccgg cctcgtcaaa gtcaatccga gtgacggttc gctcgtcacg
     1381 gccttcgatg cccatctgcg cccgaacact ggccagggcc agtacggcgc ctacgacggc
     1441 gaggaaatca tcacgatggc gccggggccc aacgcgggcg agatcttggc cggcagcggt
     1501 ggccacgcac cagtgggcga ctcatccaac gaagccctcc tgctcgacgt caacaccggc
     1561 gcccggctga tgcattactc gacgctcggg gactgccaag ggatcggcac ggtcggagac
     1621 actaccgtcg cgggctacca caacaacacc gcctccgcga gcaacccgct gtccgcgaac
     1681 tacttcggca tccaactcga gaactcgggc ggcgctaact ccctcggcgt tccgacgacg
     1741 tgggatccgc gagtcaacgg caatcaaggc aacgcggacg gcgggaacaa cggcatccag
     1801 gcgatgtacg tcgaccaagg gactcaaacg atctacctcg gcggcgcctt cctgcactgg
     1861 aacggcacga ccggttcgaa ccatcagtcg ctgattgcgt tctcgtttgc gccgtcgaat
     1921 ccgaccgcgc cgggctcgcc gaccggtgtg acagcgactg ccggcgacac gaccgcaact
     1981 gtgtcgtgga cagcgccgtc cgacggtggg agcccgatca ccgggtacac cgtgacgtct
     2041 gacccgggcg cgattgtcgc gacgaccacc ggtgcgacct cggtggttgt gcctggtctg
     2101 accgatggcg ttacgtacac gtttaccgtg acagccacca acgcaatcaa tacctctggc
     2161 tcgtcggcgc cctcgaatcc ggtcaccccg acagccggca ccttgcagtc gaactcggcc
     2221 gagggcggta cgcccggtgc ctcggtgacc acggccaact ctggcggcgc atcggggacc
     2281 gcctttacca tcgtcagcaa gggcagcggt gcgacgatca cgtacgccgc cgcggccgcc
     2341 gtccacggcg cgttggggta tgcgctcacg gggtcctcgg gcactgcgac cctgatgggg
     2401 tggaacggtt acagcgcgac gtcgatggcg atccggttct actacaaccc cgggtcgaca
     2461 ttgccgaacc aggtgctgcg gttggctgac attcgcaact cgacggcgac cgccgcgcga
     2521 gtcgagctgt cggcgtccaa ccagatcttc atccagaaca acgccggcac gacggtgacc
     2581 acgttcgccc acgcgctgca ggcgaacacc tggtaccgga tcgagatggc gatctcggtg
     2641 tcgtcgtccg ccgcatccat caatgctgcg tactacacgc tcgactcgac aactcctgtc
     2701 gatccggcgt acacgactgc gacaggtaac accggcacgg cgagcatcac tcaggtgacg
     2761 gttggctcga cggctagtgc gacctggact gggacgagct acttcgacga catcgcctcg
     2821 cagtcgccgt ccaccgcctt catcggacct gtctcggcaa gcccgacggc gcctgatgca
     2881 ccgaccgcgg tttccgcggt cccaggtgac gggcaggcga cggtgtcgtg gactgctccg
     2941 ttgtccaacg gcggtagcgc gatcaccggc tacaccgtga cgtcgtcgcc gggcgggttc
     3001 accgcgacga ctgctgggcc gacgtcggcg atcgtgagcg gcctcaccga cggcacgccg
     3061 tacacgttca ccgtcacagc gaccaacggc gtaggcaccg gaccagcgtc ctcaccttcg
     3121 tcgtccgtga ttcccgttgc tgcgccgact gcgcctggcg cgccgactgg cgtgaacgcg
     3181 accccgggcg acgggcaagc gacggtgtcg tggactgctc cgctgtccaa tggtggtagc
     3241 gcgatcaccg gctacacggt gacgtcgtca ccgggtgggt tcaccgggac tagcgctggg
     3301 gccgctacca cggcggtggt gaccgggctg gctgatggca cgtcctacac gttcacggtc
     3361 actgcggcca acagcgtcgg caccggagcg gcatccacgc cttcgcccgc cgtgatgccg
     3421 gttgcggcac cgacggcgcc tggcgcgccg acggccgtga gtgcagtccc gggtgacggg
     3481 caggcgacgg tgtcgtggac tgctccgtcg tccaacggtg gcgctgcgat caccggctac
     3541 acagtgacgt catcgcccgg tggagtcacg gtgtcgggtc cagctagccc cgcgaccgtg
     3601 accggtctga ctgatggcgt ctcctacacg ttcaccgtga ctgctgctaa cagcgtcggc
     3661 acaggtcccg cgtccgcgcc ttcgaccgga gtgattccgg ccgcggcggc gacggcgccg
     3721 ggtgcgccga ctgctgtgag cgcggttccc ggtgaccggc aagcgaccgt gtcgtggacc
     3781 gctccgttgt ccaatggtgg aagtgcgatc accggataca cggtgacgtc aacgccgggc
     3841 agtttcaccg cgaccacggc tggcgcgacg tcggcggccg tgaccggtct cagtgatggc
     3901 atctcgtaca cgttcacggt tactgccgcg aacaccgtcg gcaccggacc ttcatcgtcg
     3961 ccttcgcctg cggtgacccc ggcgacgaca cctggcgcgc ccacgggcgt gacggggacg
     4021 cctggcgaga cgcaagcgac ggtgacgtgg acagctcccg tttccaatgg tggaagtgcg
     4081 atcacgggtt acaccgtgac tgcatcaccg ggtgtggcca ctgccacgac agcgggcgcg
     4141 acgtcggcgg tagtgattgg cctgacggac ggaacgccgt acacgtttac cgtcacggcc
     4201 acgaatggca acggcgccgg accagcgtct gacccgtcgc cgtctgttgt tcccggtccg
     4261 accgttcctg atgcgcccac cggtgtgagc gccgttccgg gtgacacgca agcaacagtg
     4321 tcgtggactg ctccggcgtc caacggcagc ccgatcacga actacacagt gttgtcgtca
     4381 ccgggcggtt tcatggccac cggcgcaact agcccgctga ccgtcaccgg tctgacagac
     4441 ggcacgtcgt acacgttcac cgtcaccgcg acaaacggcg tcggcacggg agccgcgtcg
     4501 ctcccgtcgt cgtccgtcat tccgaccgcg gccgcaaccg cgcctggtgc accgaccggc
     4561 gtgaccgcaa cccctggcaa cacccaggcc acggtgacgt ggaccgcgcc atccaacggt
     4621 ggcagcgcga tcactggtta cacggtgacg tcgtcgccgg gtggattcac cgcgaacagc
     4681 gctggcccca cgtccgcgct ggtgtccggg ctgaccaacg gcacgaccta caccttcacc
     4741 gtgactgcgg ccaacggcgt gggtaccggt ccggcgtcgg gctcgtccaa caccgtgact
     4801 ccgtcggcag ggacgttcgt ctcgaactcg gctgagggcg gcatatcggg cacgtcggtg
     4861 accaccggca actcgggcgg cgcctcaggc aatgcgttga ccgtcgtgac caagggcgcc
     4921 ggcgctgcgt tggtctactc gaccgccgca gccgcacacg ggtcgctggg atacgcgctg
     4981 accggtgcgt cgggcacagc gacgctgctg ggctggaacg ggtacaccgc tccgtcgatt
     5041 gccgtccggt tctactacaa ccccgggacg acgttgccga acgcagtgct gcggctggcc
     5101 gacatccgca actcaacggc taccgccgcg cgggtggagt tgtcggcgtc caaccagctc
     5161 ttcatccaga acgccgctgg agcgaccgtg acgacgttcc cgcacgcgct gcaggccaac
     5221 acctggtacc gcgtcgagat cacgatctcg gtatcggcgt cgacggcgac catccaggcc
     5281 gcgtattacc tcagcagctc gacggcaccg gtcgacccgg cgtactcgac ggtcacgggt
     5341 aacaccggca cgacgaacat cacccaggta tcgatcggtt cggcggccag cgcgacgtgg
     5401 gtcggcacca gctacttcga tgacttggct gttcagtcgc tgtcgaccgc gttcatcggg
     5461 ccctgaccgg tcgcgcgcta gcgcttcgcg gctctgacgt cagatgcctc ggggcaacgg
     5521 gatcagcagg ttgttgttcg acggcaggcg ggtcagatcg atctgccggc cgttggactc
     5581 cagcgctcgg tagatcaggt ctgcgacgtc ggcaatcgcg tcgacgagtc ggtagtcgcg
     5641 ctgcccgtta ctcgaccagc cgccggtagc gccgttcacg tacggatcgc catccgccat
     5701 cacgccgatg aaacggttct gcatcgggcg ggggacgtct tccgcggctg agacaccgat
     5761 gacgtcagcg tccggattga gttggtcgat ctcgacccgg tcgaagatcg gggtgtcact
     5821 cgcgacccac accagcggcc gtccgtcgac gcggatgccg tagtagacgc gctggccggc
     5881 acgtaccggt accggacgct ccagcgcaaa cgcctgccca cgtgatccga tgacgcttcg
     5941 cacggtcatg tcgcgcgtcg acagcatttc tgtcatgagt cgcccccacc tagagtcccg
     6001 acgtcaaagt agtcacgcag ctcgtacggc ggtcctacgt cggtgaaaca gttgtggaac
     6061 agcgggcaaa tgacggctct actacccggc gctaagcgcc cggactactc gtcggtccgg
     6121 tggtagccga agaagccgcg cttcttgatg agctcgccca cctcgggcgg caccatggtc
     6181 tcccaggtga cgtcaccgct cgcgatccgc cgcaacacgt cgcggctgag gatcggcagg
     6241 tagtccggct tgtagttgtc caggccgacg aaactgcctc gcctcgccag gtagttatag
     6301 aggggttgca ggtcctcgcc gaccgaaagg tcgtcgacgg tcgtgatccg ttcgtcacgc
     6361 agcatcgggt agacgaagag cttcaggtcg ttcttgaaca gccggccgaa gctctcgagg
     6421 atgccgccgg gcagtgccgc gtggttgctc tcgttgaaca gatccatcag gctcgggatc
     6481 cccatgacca tgccgatgtg cccgtcggtg cgccaagcga ggtacgcggc cagccggtgg
     6541 tactccacgt agtcggagat caacaccgtc atgccacagg ccgccagcag atccgcccgg
     6601 gccaggaagt cgcgccggtc gacgtcgccg cccccagcga gcagattcgc catcgtcagc
     6661 tcggtaagca tcaggatttc gcggctcgcg acgtccgggt cggaaatgaa cttcgccagc
     6721 gctgactcga gcatgtcgat gttgacgacg gtgggcggcc tgaaactccc acgctcgacg
     6781 aggatcgcct tcttccgtaa cacctcgctc ggctggagca cctcgcggtc gggtccgaac
     6841 atcgccgcgc cactcaagcc gacctgcacg agcttcagcg ccatcagccg gttgtcgacc
     6901 gagcggaact cgatcccctt caactcgatc atgtcgatct ctatgcggcc cgtggtcaac
     6961 ctgtcgagca ggctttcgac cagcatttcc ggctcgtggt gttggaagaa cgcggcgtgc
     7021 agcaagttga cgccgacgat gccgagcgct tcctgctgga gcgctgcttc gacgtccagc
     7081 atgcggacat gcatgatgat ctggcttggc tcatcgtgcg gatgagcttg gaacttcacg
     7141 cccatccagc cgtggcactc gttgccgccg ctgaaactgc gcgcggttac ggtgtcggca
     7201 aacgcgaaga aggcattcga gtcgccgcgc tcggcgtcga ggcgctccag gttgagcccg
     7261 aattcgaggt caagcatggc ctgcagacgc ggcatcgaaa catacctgcc ggacttgccg
     7321 tacacggcat cgctcacggc catgtcgtac gccgagatc
//
LOCUS       DATCBJ010000594         3933 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_201385, whole genome shotgun sequence.
ACCESSION   DATCBJ010000594 DATCBJ010000000
VERSION     DATCBJ010000594.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 3933)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3933)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3933
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_201385"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(63..461)
                     /locus_tag="VII50_11945"
     CDS             complement(63..461)
                     /locus_tag="VII50_11945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HEY5051606.1"
                     /translation="MTTTSGRSTRQKRALAVVIDDAQEFRSAQELHEALRGRGEHVGL
                     TTVYNQLRALADAGLVDTMRSPEGEILYRRCGSAGHHHHLLCRVCGRTVEVAGTAVER
                     WAEKVAAEAGFVDVSHTMEVVGTCPTCALL"
     gene            677..1561
                     /locus_tag="VII50_11950"
     CDS             677..1561
                     /locus_tag="VII50_11950"
                     /inference="COORDINATES: protein motif:HMM:NF013465.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HEY5051607.1"
                     /translation="MALVLVSGCTTSVRRVTFLDGPPRVVAAENFWGSIATQLGGDKV
                     GVESIITNPDTDPHDYDPSATDARALANANVVIENGIGYDPWVSKLLAADHVSDQHVI
                     NVGTLLGIADGGNPHRWYNPTDVHTVIAAITAAYAAADPADAKYFAAQQKNYETKSLA
                     QYTSLINQIKTTYAGTPVGASESIFAMIAPALGLDLLTPPAFLRAISEGTDPTSADKQ
                     TIDNQIKTHTIKVYVYNSQNATPDVQAQVDEAKAAGIPVTTITETLSPASATFQDWQV
                     AELLALQTALKTAKGVGS"
     gene            1558..2403
                     /locus_tag="VII50_11955"
     CDS             1558..2403
                     /locus_tag="VII50_11955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY5051608.1"
                     /translation="MTSTPAAQLPSAVQLRDAAVRVGDRLLWHDVNVDVPAGQFVAIL
                     GPNGVGKSTLIKAVLGLIPLAAGEAFVLGQPAGMANRDIGYLPQRRSFDPSLRVRGID
                     VVRLGLDGDRFGLPLPWGAKNREAGRRVRDVIERVDASSFGLRPIGQLSGGEQQRLLI
                     AQALVRDPQLLILDEPLDSLDLPNQSAIADLIRRVCVDGGVTVLMVAHDVNPILPYLD
                     QVMYLAGGGVVCGTPAEVITSPTLSALYQTPIEVLRTSDGRLVVVGQPEAPAHHADRH
                     TGRPH"
     gene            2403..3278
                     /locus_tag="VII50_11960"
     CDS             2403..3278
                     /locus_tag="VII50_11960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HEY5051609.1"
                     /translation="MTVAASIASGNVAWSWNLLGDVRDMFAYPFMVNAFRAGTIVAIV
                     GAVVGWYMVLRKQTFAGHTLSVAAFPGAAAATLIGISATVGYFTFCLAAALVIAIASR
                     PSRGRGFSAESAVVGTVQAFVLASGYLFATLYNGLLSGLNNQLFGFTFGITNDQVVTL
                     GVVAVIVLAVLAVIGRRLFFASIDTDVADARGVGVRLLGVAFLLVLGAAAAEASQITG
                     ALLVFALLVMPAAVAQRVTARPVAGIVLAVLIGVVATWGGLTMAYYTSYPSGFCITTF
                     AFAAFIAVRRVRAWA"
     gene            3278..>3933
                     /locus_tag="VII50_11965"
     CDS             3278..>3933
                     /locus_tag="VII50_11965"
                     /inference="COORDINATES: protein motif:HMM:NF013143.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HEY5051610.1"
                     /translation="MAPYMQHAALAGTAIAIAAGLIGYFLVLRSQVFTTDALGHVTYT
                     GAMSGLAFAFNPLLGLFAATVAVAGGFAVLGRRARADDVVIGSTFAWVLGLGALFQRL
                     YTEHHSSGNGRAGATYLFGSIFGISSTQAVVSVVICAAVCLAMALIARPLLFSSIDEA
                     VAVARGVPVRLLGAGFLLVVGVTCAQAAQAVGALLLLGLISAPAGAAHRLTTRPFVGL
                     "
BASE COUNT          629 a         1303 c         1260 g          741 t
ORIGIN      
        1 cgggcgactc cgcacgtgct cagaacgaca acggctagca ctacgcggct agcactacac
       61 ggctagagga gagcgcaggt cgggcaggta cccacgactt ccatcgtgtg tgagacgtcg
      121 acgaagccgg cttcggcggc aaccttctcg gcccagcgct cgactgcagt acccgcaacc
      181 tccaccgtcc ggccgcatac ccggcacagc aggtggtggt ggtggccggc actgccgcac
      241 ctgcggtaga ggatctcgcc ttcaggcgac ctcatagtgt cgacgagacc ggcgtcggca
      301 agcgcgcgca actggttgta gaccgtagtc agcccgacgt gttcccctcg tccgcgcaac
      361 gcctcgtgca actcctgcgc actgcggaac tcctgcgcat cgtcgataac cacggcgagc
      421 gcgcgcttct ggcgggtcga tcggccgcta gtcgtcgtca tggcgacacc aaatctagcc
      481 gaccccagca gggcgttgca tcgcacttgg aaacggtaac cattcccgat agtgtacttg
      541 caacggttcc ggataagcgg atccgcggcg acgggtggac agagtgcagg tcttcagccg
      601 gacggcagtg cgccacccga gatcgtggac gtttgccgcc ccaggagggc ggcaaacgtc
      661 gcttttgttg gtagccgtgg ctctagtcct cgtgagcggc tgcacgacat cggtacgcag
      721 ggttacgttc ctggacgggc cgccgcgcgt tgtcgcggcc gagaacttct ggggctcgat
      781 cgcgacacag ctcggcggtg acaaggtcgg ggtggaaagc atcatcacga atcccgatac
      841 agatccgcac gactatgacc caagcgcgac cgacgcgcgc gctctcgcga acgccaacgt
      901 cgtcatcgag aacggcatcg gctacgaccc gtgggtgagc aagctgctcg ctgccgacca
      961 cgtcagcgac caacacgtga tcaacgtcgg gaccctgctc gggatcgcgg acggcggcaa
     1021 tccgcaccgc tggtacaacc caaccgacgt gcacaccgtg atcgccgcga ttaccgctgc
     1081 ctacgccgcc gcggacccag ccgacgcgaa gtattttgct gcccagcaaa agaattacga
     1141 gaccaagtcc ttggcccagt acaccagcct gatcaaccag atcaagacga cctacgcagg
     1201 cacgccggtg ggagcgtcgg agagcatctt cgcgatgatc gccccggcac tgggtctgga
     1261 cctgctgacg ccgccggcct tcctgcgggc gatcagcgaa ggcaccgacc cgacgtcggc
     1321 cgacaaacag acgatcgaca atcagatcaa gacgcacacg atcaaggtct acgtctacaa
     1381 ctcgcagaac gccactcccg acgtgcaggc gcaggttgac gaggcgaagg cggctggcat
     1441 cccggtgacc acgattaccg agactctttc gcccgcctcg gcgacgttcc aagactggca
     1501 agtcgctgaa ctgctcgcgt tgcagactgc cctcaaaacc gcgaaaggag tcggttcatg
     1561 acttcgaccc ccgcagcgca gcttccttcg gcagtgcagc tgcgcgatgc ggcggtccga
     1621 gtcggcgacc ggctgctctg gcatgacgtc aacgtcgacg ttccggccgg tcagttcgtc
     1681 gcgattctcg gaccgaacgg cgtcggcaaa tcgaccctga tcaaggccgt gctaggcctg
     1741 atcccattgg ctgccggcga ggccttcgtg ctcggtcagc cggccggcat ggctaaccgc
     1801 gatatcggct atctccccca gcgacgcagc ttcgatccgt cgctgcgggt gcgcggcatt
     1861 gacgtcgtgc gccttggcct cgacggcgat cgcttcggtc tgccactgcc gtggggcgcg
     1921 aagaaccgtg aagcgggacg ccgggtgcgc gacgtgatcg agcgagttga cgcgtcgtcg
     1981 ttcgggctgc gtccgatcgg gcagctctcc ggcggcgagc agcagcggct gctcatcgcg
     2041 caagcactcg tgcgcgatcc gcagctgctc atcctcgacg agccgctcga cagcctcgac
     2101 ctgcccaacc aaagcgcgat cgcggatctc attcgccggg tgtgcgtcga tggcggcgtg
     2161 accgtactca tggtcgccca cgacgtcaac ccgatcctgc cctacctcga ccaggtgatg
     2221 tatctcgccg gcggcggcgt ggtatgcgga acacctgcgg aggtcatcac ctcaccgaca
     2281 ttgtcagcgc tgtaccagac gccgatcgag gtgttgcgca cctccgatgg ccggctcgtc
     2341 gttgtcgggc aaccggaggc gccggcgcat cacgccgatc gtcacaccgg gcggccacac
     2401 tgatgaccgt cgctgcatcg attgccagcg gaaacgtcgc gtggtcgtgg aaccttcttg
     2461 gcgacgtccg agacatgttc gcgtacccat tcatggtgaa cgcgtttcgc gcaggcacga
     2521 tcgtcgcgat cgtgggtgca gtggtcggct ggtacatggt gttgcgcaag cagacgttcg
     2581 ccggtcacac cttgtcggtc gccgcctttc cgggcgcggc tgccgcaact ctgatcggca
     2641 tcagcgcaac tgttggctac ttcacgttct gtcttgccgc cgcactcgtc atcgcgatcg
     2701 catcgcggcc atctcgtggc cgcggattca gtgcagagtc ggctgtcgtc ggcactgtcc
     2761 aggcattcgt gctcgcgtcg ggatacttgt tcgccacgtt gtacaacggc ttgctgagcg
     2821 ggctcaacaa tcagctgttc ggcttcacgt tcggcatcac caatgatcag gtggtgacgc
     2881 tcggcgtggt cgccgttatc gtgctggcgg tacttgcggt catcggccgc cggctcttct
     2941 tcgcatcgat cgatactgac gtcgccgacg cgcgcggtgt cggcgtccgc ctcttaggtg
     3001 ttgcgttctt gctcgtgctc ggtgctgctg ctgcggaagc cagtcagatc accggcgcgc
     3061 tccttgtgtt tgcgttgctc gtcatgccgg ccgcagtcgc acagcgggtg acggcacggc
     3121 cggtggcagg aatcgtgctc gccgtgctga tcggtgtcgt ggcaacgtgg ggtggcttga
     3181 cgatggcgta ctacacgtcg taccccagcg gcttttgcat cacaacgttt gcgtttgccg
     3241 cgttcatcgc ggtgcgtcgc gtgagggcgt gggcgtgatg gcgccgtaca tgcagcacgc
     3301 ggcgttggcc ggcaccgcga tcgcgatcgc cgcggggctc atcggctatt tcttggtgct
     3361 gcgttcgcag gtgttcacga cggatgccct cggccacgtc acctacaccg gtgcgatgag
     3421 cggtcttgca ttcgcgttca atccgttgct tggactgttc gccgcgactg tcgccgtcgc
     3481 cggcggcttc gctgttctcg gacgccgcgc gcgagccgat gatgtcgtga tcggctcgac
     3541 gttcgcgtgg gtgctcggcc tcggcgcgct gtttcaacgc ctgtacaccg aacatcacag
     3601 cagcgggaac ggacgcgcgg gcgccacgta cctgttcggt tcgatcttcg ggatctcctc
     3661 gacgcaggcg gttgtgtcgg tcgtcatctg cgcggccgtc tgcctcgcga tggcactcat
     3721 cgcgcggccg ttgctcttct ccagcatcga tgaagcagtc gcggtggcgc gcggggtgcc
     3781 ggtgcgacta ctcggcgcag gattcctgct ggtcgtcggc gtgacctgcg cccaggccgc
     3841 gcaggcggtc ggcgcactgc tcttgctcgg ccttatctcc gctccggcgg gcgcggccca
     3901 tcgcctcacc acgcgcccct ttgtcggcct gag
//
LOCUS       DATCBJ010000595         2075 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_201510, whole genome shotgun sequence.
ACCESSION   DATCBJ010000595 DATCBJ010000000
VERSION     DATCBJ010000595.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2075)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2075)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2075
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_201510"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..223
                     /locus_tag="VII50_11970"
     CDS             <1..223
                     /locus_tag="VII50_11970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5051611.1"
                     /translation="GAMVVRDLDGAAVHDRQAEDAFWAEVATRRAVVAAEVATRREAL
                     MAKRLTQWTGRSRERDCDARLAGLPLRPC"
     gene            902..1258
                     /locus_tag="VII50_11975"
     CDS             902..1258
                     /locus_tag="VII50_11975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5051612.1"
                     /translation="MSTVDMWGYPDEAAVDRDLTGWSIEAEDGGIGKVARANNEAGAS
                     WLIVDTGPWIFGKKVMLPAGVIARINSENEVVHVNRTKEEIKNAPEFDEGRYQEQTYR
                     DELGGYYGPGGRGYRQ"
     gene            1519..>2075
                     /locus_tag="VII50_11980"
     CDS             1519..>2075
                     /locus_tag="VII50_11980"
                     /inference="COORDINATES: protein motif:HMM:NF013715.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HEY5051613.1"
                     /translation="MSDSTTKGGGRRRRRTRVDTTQAASPGYLRGYLRALGPGLVTGA
                     SDDDPSGIATYAQAGASFGFGFLWTALLTFPLMATVQEICDRTALATGRGLGELATVR
                     LGGKARSIVIGLVGLLIVANALNIAADLVAVGSGMQLLHAGPTAVWAVIAGVGISALL
                     LTGSFELVARVFKLLCIALVAYIAM"
BASE COUNT          351 a          625 c          725 g          374 t
ORIGIN      
        1 cggcgccatg gtcgtccgag acctcgacgg cgcggccgtc catgaccggc aggccgagga
       61 tgcgttctgg gccgaagtag ccacgcggcg cgcggtcgtc gcagccgagg tcgcgacgcg
      121 gcgcgaggcg ctcatggcca agaggctgac gcagtggacc ggacgttcgc gtgaaaggga
      181 ctgcgatgcc cggctcgccg ggctcccgct tcgaccgtgc tgacgtttac aggtctcccg
      241 gcactttgca agcaccgcga gctcggccgg cggcgcggca gtttgcagga tcgcctgacc
      301 gcagcaaatc gtcggcatcc gagcgactgt cgcatcccga ttgccgagca gcagcaccgc
      361 gtcagccgtc cggcagcaca cgcgcttgtc cataccagca cagcactagc tggaggtcga
      421 tgagcacttc cgcatttgcg gccgcgcgtt tgctgaccga cccagctgca agcaccctgc
      481 gtttgcaaga tgatcgcgac gcagctaacg agtggcgtca tcctacctcg ggccagccaa
      541 gctcggaatg agcgttcggc agcggcgctc gcgccggccg acgaggtgtc caacgagtgg
      601 gtctgtcgct cggtcgagcg gtatcgcgcg cagatccgcg agcacttggg gttcgtagtc
      661 ttgacgctgc gcgggacttg ttcgatcacg gacgtattcg tccgtaccta ggatgcttag
      721 gagacgtccg caggggctaa gttgcgcgtg ctcgcagctt ggcgacggcg gctcagcgtc
      781 agacccatca tcgccgagtg tgagcgccgc ggttggcagc tggtccacac atcgatgctg
      841 tctagtcgcg cgcggttttg aatctgtcag ccacgacgga agtgaaagaa ggtgatggtt
      901 ggtgagtact gtagacatgt gggggtatcc cgatgaggct gccgtcgata gggatttgac
      961 tgggtggtcg atcgaagccg aggatggggg tattggtaag gttgccaggg ccaacaacga
     1021 ggcgggcgca agctggttga tcgttgatac ggggccatgg atcttcggca agaaggtcat
     1081 gctgcccgcg ggtgtgatcg cccggataaa cagcgaaaac gaggtagttc acgtcaaccg
     1141 caccaaggaa gagatcaaga acgctcccga gttcgacgaa ggtcggtacc aagagcagac
     1201 gtatcgcgac gagcttggtg gctactacgg acccggcggt cgcggctacc ggcagtgagt
     1261 ctcgcttgtc ctgccacact cgcactccga tacacggctg gagaccccgg acccgcactc
     1321 gattcctcgc ccggggcgct ccagcaggcc acgccgtctc gcgtctcgcc tagtcaagct
     1381 cggcaccgcg cagggtctga gtcacgcgca gcgacgtgtc aacgccaagc tgccacaagc
     1441 ggacagcgac acgcggagcg gacgggccgg gatgaccgcc gtcacatcta acctctacat
     1501 gctcgggaca acctcgctgt gagtgactcg accactaagg gcggcgggcg ccgtcgtagg
     1561 cggacgcggg tcgacaccac acaggcggcc agccccgggt acctgcgtgg ctatctgcgc
     1621 gcgctaggac cggggttggt gactggtgcc tctgacgatg acccgtccgg gatcgcgacc
     1681 tacgcgcagg ccggggccag cttcggtttc gggtttctct ggacggcgct gctgacgttt
     1741 ccgttgatgg ccacggtcca ggagatttgc gatcggacgg cgctagccac cgggaggggg
     1801 ttgggcgagc tcgcgacggt gcggcttggc ggcaaggctc gtagcatcgt gattggcctg
     1861 gtcgggttgc tgatcgtggc gaacgcgctg aacatcgcgg ctgacctggt ggcggtcggc
     1921 tcgggcatgc agttgctcca tgcagggccc acggctgtct gggcggtgat cgcgggcgtg
     1981 ggcatcagcg cgctgctgct gactgggtcg tttgagctcg tcgctcgggt cttcaagctg
     2041 ctctgcatag cgcttgtggc gtatatcgcg atgtt
//
LOCUS       DATCBJ010000596         4593 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_64347, whole genome shotgun sequence.
ACCESSION   DATCBJ010000596 DATCBJ010000000
VERSION     DATCBJ010000596.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 4593)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4593)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4593
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_64347"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..914
                     /locus_tag="VII50_11985"
     CDS             <1..914
                     /locus_tag="VII50_11985"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HEY5051614.1"
                     /translation="TGRGIGPAYSDKMSRVGVRVQDLFDEKILRQKVEGALKPKNQLL
                     VKIYNRAAIDTSAVVTELLEYADRLQPMVQDTSLLLNNALDRGETVLLEAGQATLLDV
                     DHGTYPFVTSSNATAGGACTGSGIGPTRIDRVIAVVKAYTTRVGAGPFPTELEDADGE
                     KLRAIGAEFGTTTGRPRRCGWYDAVIARYAARVNGVTDFVLTKLDVLSDFDTIPVCVG
                     YRLPDGSVIDEMPMTQTDFHHAQPVYEEFPGWREDISKAREFDDLPKNARRYLEALQE
                     MSGAPFSVIGVGPARDQTLLLRPLTAG"
     gene            928..2181
                     /gene="purD"
                     /locus_tag="VII50_11990"
     CDS             928..2181
                     /gene="purD"
                     /locus_tag="VII50_11990"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HEY5051615.1"
                     /translation="MNILIIGGGAREHAICLALRDDPAVTTLHCAPGNAGIATIAHTS
                     EIDVRDPDAVAKLAATLKADLVVIGPEIPLVAGVVDAVQAQGIACFGPTRAAAQLEGS
                     KAFAKEVMAAADVPTAASRVCTDARQAAAALDEFGPPYVVKDDGLAAGKGVVVTLDRN
                     VALEHASDCTRVVIEEYLDGPEVSLFAITDGTTVVPLLPAQDFKRLADDDQGPNTGGM
                     GAYAPLPWAPNALVDEVMRDVLQPTVDEMRRRGSPFAGLLYAGLALTSRGTRVVEFNA
                     RFGDPETQVVLALLQTPLAGLLNAAATGALADHPPLMWRDGYAVTVVVAAAGYPESPR
                     GGDPIGGLDQVTLPAYVLHAGTNPRGGQTVATGGRVLSVTATADTLAAAREAAYAGVA
                     QITLNGGHHRSDIALLAVRGEIRLP"
     gene            2248..2772
                     /locus_tag="VII50_11995"
     CDS             2248..2772
                     /locus_tag="VII50_11995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013230782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SigE family RNA polymerase sigma factor"
                     /protein_id="HEY5051616.1"
                     /translation="MRAANEAEFKAYAAARMRDLRRTAFLLCGDWHHADDVVQTVFTK
                     LYVNWDKVQRHDRLDGYVRTMLVRATFDRRRRLSWRRELSSAAPPETAAIAPSSVEDR
                     LLLFDALAKLPPRQRAVVVLRFWQDLDVTETAHLLGCTEGTVKSQSARGLAKLRELLA
                     PEPTTAELMEGQPR"
     gene            2769..3836
                     /locus_tag="VII50_12000"
     CDS             2769..3836
                     /locus_tag="VII50_12000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5051617.1"
                     /translation="MNDEDFARHAFAAAFRSGQTGEPPTLPDVELLARHGRRANRSRH
                     GVYAAGTTVLAGVAVAGVVTGPALLGLGSTSPSDISAGAGTGTTTPPSSPAPSSSSSA
                     PDPAKPSPGAPCSTSSTLNWLSVVTAALPKGVTATSDHLANCVQLADGSRSYEALFKL
                     STGAVELQVNVGTGPDIAAKLGAGTVKIGIAPASESPSESLDPAALASLRASKMAAAA
                     AAAGDSSASAVGSPPPSPSLAPSVDAAALASLEAHKRAAASAAATASPAPGDAPAGGD
                     KGVAGGSCSKVGPAENACVSHMSKNSLSVVDVELLRTGGSPVVVDVTASNGKNLAAAA
                     PGRLPSDATMVALAQAVAAHF"
     gene            3892..>4593
                     /locus_tag="VII50_12005"
     CDS             3892..>4593
                     /locus_tag="VII50_12005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003095989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lyase family protein"
                     /protein_id="HEY5051618.1"
                     /translation="MTTPQIPDVLATRYASAALVELWSPRAKVALERQLWIAVLKAQA
                     ELGIDVPAGVVDDYERVVANVDLDSINAREKVTRHDVKARIEEFNALAGHEHIHKGMT
                     SRDLTENVEQLQIRESLVLIRDRMVAALARLARLAAEHAELVIVGRSHNVAAQATTLG
                     KRFASAGDELLIALERIEQLIARYPLRGIKGPMGTAQDMLDLLDGDAAKLAQLEASVA
                     GHLGFERVLNSVGQVY"
BASE COUNT          832 a         1546 c         1483 g          732 t
ORIGIN      
        1 cgacgggccg cggcattggg cccgcctact ccgacaagat gtcgcgcgtc ggcgtgcgcg
       61 ttcaggacct tttcgacgag aagatcctgc gccagaaagt cgaaggcgct ttgaagccga
      121 agaaccagct gctggtgaag atctacaacc gcgcggccat agacaccagc gcggtggtca
      181 ccgaacttct cgagtacgcg gatcgcctcc agccgatggt gcaagacacc agcctgctgc
      241 tgaacaacgc gctcgaccgc ggcgagacgg ttctgctcga agccggccag gcgaccctgc
      301 tggacgtcga ccatggcacc tatcccttcg ttacgtcgtc gaacgccact gctggcggcg
      361 catgcacagg ttccggcatc ggcccaaccc gcatcgatcg cgtcattgcg gtcgtcaagg
      421 cctacacgac gcgcgtgggt gccgggcctt tcccgacaga acttgaagat gcggacggcg
      481 agaagctccg cgctatagga gcagagttcg gcaccaccac cggccgacca cggcgttgtg
      541 gctggtacga cgcggtcatc gcgcgctacg ccgcgcgagt caacggagtc accgacttcg
      601 ttctgaccaa gctcgatgta ctgtccgact tcgacacgat cccggtgtgt gtcggctacc
      661 gacttccgga tggcagcgtc atcgacgaga tgccgatgac gcagaccgac ttccaccatg
      721 cgcagccggt ctacgaagag ttcccgggct ggcgcgaaga catttcgaag gcgcgggaat
      781 tcgacgacct gcccaagaat gcgcgcaggt acctcgaggc gttgcaggag atgtccggtg
      841 cgccgttcag cgtgattggc gtcggccccg cgcgcgacca gaccttactg ttgcgaccgc
      901 tcactgcggg ctgaaccgcc gccacccgtg aatatcctca tcatcggtgg tggtgcgcga
      961 gagcatgcga tctgcttagc gctgcgcgac gacccggccg ttacaacact gcactgcgca
     1021 cccggcaatg ctggcatcgc caccatcgcc cacaccagcg agatcgacgt ccgagatccc
     1081 gacgcggtcg ccaaactcgc agcaaccctc aaggccgatc tcgtcgtcat cggccccgaa
     1141 atccccttag tagcaggggt tgtcgacgcc gtacaggcgc aaggcatcgc atgctttggc
     1201 ccaacccgcg cagccgcgca gctcgaaggg agcaaggcgt tcgcaaaaga agtcatggct
     1261 gccgcggacg tccctactgc tgcttctcgt gtctgtactg acgcccgaca ggcagcagcg
     1321 gcgctcgacg agttcggtcc accgtacgtg gtcaaggacg acgggctcgc cgctggcaaa
     1381 ggtgttgtag tgacgctcga tcgcaatgtg gcgctcgagc acgcaagcga ttgcacgcga
     1441 gtggtcatcg aggagtacct cgacgggccc gaggtgtcac tgttcgccat caccgacggc
     1501 acgactgtcg tcccgctgct accggcgcaa gatttcaagc gactagcgga cgacgatcag
     1561 gggccgaata ccggaggcat gggtgcctac gcgccgctgc cttgggcacc gaatgcgctg
     1621 gtcgacgagg tcatgcgcga cgttctgcaa ccgacggtcg acgagatgcg acgacgtggc
     1681 tcgccgttcg ccggtctcct gtacgcggga ctcgcactga caagtcgagg aacgcgcgtc
     1741 gtcgaattca acgcccgctt cggcgatccc gaaacgcagg tcgtcctcgc gctactgcag
     1801 acgccactag cgggtctgct aaacgcagca gcgaccggcg ctcttgccga ccatccgccg
     1861 ctgatgtggc gcgacgggta cgcggtcact gtcgtcgtcg ccgcagccgg ataccccgag
     1921 agtccgcgtg gcggcgatcc gatcggcggc ctcgaccagg taacactgcc cgcctatgtc
     1981 ctgcacgcgg gtaccaaccc ccgaggtggc caaaccgtag ccaccggggg gcgcgtcctg
     2041 tcggtgaccg ctaccgccga cacgctcgcc gcggcgcgcg aggccgccta tgccggcgtc
     2101 gcgcagatca cgctaaacgg cggacaccat cgaagcgaca tcgcgctgct cgccgtccgc
     2161 ggtgaaatca ggctgccgta gccgtgcaac ctggcgtgcc agccggcacc tgttagaggg
     2221 tgcaacgcag aggcgaaagg gaccagcgtg cgagcagcga acgaggcgga gttcaaggcc
     2281 tacgcggctg cccgcatgcg cgacctgcgc cgcacggcgt tcttgctgtg cggtgattgg
     2341 caccacgcgg acgacgtcgt gcagacggta ttcaccaagc tctacgtgaa ctgggacaag
     2401 gtccaacgcc atgatcgcct cgacggctat gtgcgcacga tgctcgtccg ggccacgttt
     2461 gaccggcgcc gccgattgtc gtggcgccgc gaactgagct ccgccgcgcc acccgaaacg
     2521 gcagcaatag cgcccagcag cgtcgaagac cggctgctgc tgttcgatgc actcgcgaag
     2581 ttgccgcctc ggcagcgagc agtcgtggtc ctgcggttct ggcaggacct cgacgtcacc
     2641 gagaccgcgc acttgctcgg ctgcactgaa ggaaccgtga agagccaaag cgcacgcggg
     2701 cttgcgaaat tgcgcgagtt gttggcgccc gagcccacca ctgctgaact gatggaagga
     2761 caaccacgat gaacgacgag gactttgccc gacacgcttt tgccgccgcg ttccgctccg
     2821 gtcaaaccgg agaaccaccg accttgcccg acgtcgagtt gctggcgcgc cacggtaggc
     2881 gcgcgaatcg aagccggcac ggtgtctacg ccgccgggac gacggtgctg gccggggtcg
     2941 cggttgccgg agtggtgacg gggcctgcat tgcttgggct cggcagcacc tcgccgtccg
     3001 acatctctgc cggtgcaggc actggcacga ccacgccacc gtcatcgcct gccccgtcat
     3061 cgtcaagctc ggcgcccgat cccgcgaagc cgtcacccgg ggcgccgtgc tcgacttcat
     3121 cgacgctcaa ttggctaagc gtcgtcaccg cggcgctgcc gaaaggcgtt accgcgactt
     3181 ccgaccactt agccaactgc gttcagctgg ccgacgggtc gcgtagttac gaggcattgt
     3241 tcaagctgtc gaccggtgcc gtcgagcttc aggtgaacgt cgggactggt cccgacatcg
     3301 cggcgaagct cggcgcgggg acggtcaaga tcggaattgc gccagccagt gaatcgccgt
     3361 cagagtcctt ggatccggcg gcgctggcca gcctccgggc cagtaagatg gcggccgccg
     3421 cagccgccgc tggcgatagc tcggcctctg cggttgggtc gccaccaccc tcaccgtccc
     3481 ttgcgccgtc ggtcgatgct gctgcactcg cgagccttga agcccacaag cgcgctgcgg
     3541 cgagcgccgc cgcaaccgct tcgccggcac ctggcgacgc accagcgggt ggggacaagg
     3601 gggttgccgg cggcagctgc tcgaaagtcg gtccagcaga gaacgcatgt gtttcgcaca
     3661 tgtccaagaa ctcgctgtcc gtcgtggacg tcgaacttct gcgcacaggt ggcagtccgg
     3721 tggtggtcga cgtcaccgcg agcaacggca agaacctcgc ggcggcagct cccggacggc
     3781 tgccgagcga cgcgaccatg gtggcgctcg cgcaagcagt ggccgcccac ttttaacccc
     3841 gactgcggcg accgacgaga ccgcgtcggc cgatgagaca atggtcgcac catgaccacg
     3901 ccccagattc ccgatgtgct cgcgacgcga tacgcctcgg ccgcgctagt cgagctgtgg
     3961 tctccgcgcg ccaaggttgc ccttgaacga cagctgtgga tcgccgtcct caaggctcag
     4021 gcggagctcg gcatcgacgt cccggctggc gtcgtcgacg actacgagcg cgtcgtcgcg
     4081 aatgtcgacc tcgactcgat caacgcacgc gagaaggtca cccggcacga cgtgaaggcg
     4141 cgcatcgaag agttcaacgc gctggcgggg cacgagcaca ttcacaaggg catgacctcg
     4201 cgtgacctca ccgaaaacgt cgagcaactc cagatccgcg aatcactggt gctcatccgc
     4261 gaccggatgg tggccgcgct ggcccggctc gcccgcctcg ccgcggagca cgccgagctg
     4321 gtgattgtcg gacgcagtca caacgtggcg gctcaggcga cgacactggg gaagcgattt
     4381 gcctcagccg gtgacgagct actgatcgct cttgagcgca tcgagcagct gatcgcgcgc
     4441 tacccgttgc ggggcatcaa aggaccaatg ggcaccgccc aggacatgct cgatttgctg
     4501 gacggcgatg cggcgaaact cgcccaactc gaagcgagcg tcgcgggcca cctgggtttc
     4561 gagcgggtac tgaacagcgt cggccaggtg tat
//
LOCUS       DATCBJ010000597         4043 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_376248, whole genome shotgun sequence.
ACCESSION   DATCBJ010000597 DATCBJ010000000
VERSION     DATCBJ010000597.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 4043)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4043)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4043
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_376248"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..280
                     /gene="cgtA"
                     /locus_tag="VII50_12010"
     CDS             <1..280
                     /gene="cgtA"
                     /locus_tag="VII50_12010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009081080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Obg family GTPase CgtA"
                     /protein_id="HEY5051619.1"
                     /translation="FTVTREGDTFVVRGERPERWIKQTDFTNDEAVGYLADRLNRLGV
                     EAELAKAGAEAGAEVIIGDVAFDWEPGMLSGSEHRIGPRGTDSRLDGR"
     gene            303..1454
                     /gene="proB"
                     /locus_tag="VII50_12015"
     CDS             303..1454
                     /gene="proB"
                     /locus_tag="VII50_12015"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HEY5051620.1"
                     /translation="MTDSTDAAGGVVRPHVAAARKIVVKIGSSSLTRPEGGLDQANID
                     ALASAIVGRLAQGTSCVLVSSGAIAAGLRPLGLTARPRDLATQQAAASVGQGLLVESY
                     ARAFGLHDVRVGQVLLTSDDMIRRVHYLNAERTLTRLLELKVVPVVNENDTVVTDEIR
                     FGDNDKLAALVAHLLRADLLVLLSDVDALYTAPPGRPGSRRLDDVHAEADLAGVDLGG
                     VGRVGIGSGGMITKVDAARLATSAGIPVVVASAAAVVDALAGKRAGTLFHPTGARVAS
                     RLLWLAHATTPRGRLVLDPGAVAAVVDRRLSLLPAGITSVEGDFAAGDPVDLVDEAGR
                     AVARGLVNYDATELPGLLGRSTRDLAAELGTAYEREVVHRDDLVLLTNR"
     gene            1540..>4043
                     /locus_tag="VII50_12020"
     CDS             1540..>4043
                     /locus_tag="VII50_12020"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEY5051621.1"
                     /translation="MTTCQHCGAEMPGDLKFCRECGSSMASVRSCSSCGAPDEGGRFC
                     GNCGATLKPGLIPAARDNPVAERRVTSVLFGDLAGFTPLSESRDAEDVRELLSRYFVE
                     CRTIIGRYGGIVEKFIGDAVMAVWGVPLSHEDDAERAVRAGLELVESTKALGERVGVP
                     GLAMRVGVVTGEVAVTVGATAEGMVAGDAVNTASRVQSVAEPGSVWVDDTTRALTAAS
                     VSYMDVGEHWLKGKAEPARLWQARAVVADLGGGQRVDGLEAPLVGREREVRLIKELFH
                     ATNESQRPRLVVLDGEPGVGKSRLAWEFEKYVDGLKAITRWHRGRCLSYGDGVAFWAL
                     AEALRPRLGLTESDAGDAVAAGLEASLAEFVSRSEERDWLRPRLAALVSGGQAGGFAR
                     EDLFTAWTAFLERISEDGNTVVLVIEDAHYADDGLLAFLESMLANANAPIFVLALARP
                     ELLTRRPDLGGRRTSVVRLDPLDDATMSTLVDGLVDGLPAVARNALVERAEGIPLFAI
                     ETVRALIDQDAVVPRDGRYVPADGVTIDLASIGAPASLQALVAARLDALSPTERAVVA
                     DASVLGTSFAREGLLALGSDRDGLDDALATLQRKEIIALQQDRFSADRGQYRFVQSVV
                     RQVAYGTLSRRDRKARHLAAADFLSAQLDANDDLAVVVAQHLLDAVDAGGASDDDTSE
                     LLPRACALLERAGIRARALGSPAEARRRFDAALARATDPAARASLYLAAADAAYDSGD
                     YEGAVAEGAAATDLFDALGRPIDAALAAATGSMGLQTLGDFAGVIRIAEPRWQALDGV
                     AGAERALLQLARPLASATGQLEDEAASGRYTQRRVLL"
BASE COUNT          570 a         1338 c         1459 g          676 t
ORIGIN      
        1 gttcaccgtc acccgcgagg gcgacacgtt cgtcgtccgt ggtgagcggc ccgagcgttg
       61 gatcaagcag accgacttca ccaacgacga agccgtcggg tatctggccg accggctgaa
      121 ccggcttggc gtcgaggcgg agcttgcgaa ggcgggcgct gaagcgggtg cggaggtcat
      181 catcggcgac gtcgcgttcg actgggagcc cggcatgctg tctgggtcag aacaccggat
      241 cggaccgcgc ggaaccgact cgcgcctcga tggccggtga ccacggtggt tcgggtgcaa
      301 cagtgaccga cagcacggac gccgccggcg gcgtcgtccg gccgcacgta gcggcggcgc
      361 gcaagatcgt cgtcaagatc gggtcgtcgt cgctgacccg gcccgaaggt ggcctcgacc
      421 aggcgaacat cgacgcgctc gcgtccgcga tcgtcgggcg actcgcccag gggacatcgt
      481 gtgtgctggt gtcgtcgggc gcgatcgcgg ccggtctgcg gccgctcgga ctcacggctc
      541 ggccgcgcga tcttgccacc cagcaggcgg cggcgagtgt cgggcagggc ctgctcgtgg
      601 agtcgtacgc gcgcgccttc gggctgcacg atgtccgcgt gggtcaggtg ttgctgacct
      661 ccgacgacat gatccgccgg gtgcattacc tcaacgcgga gcggacgctc acccgcctgc
      721 tcgaactcaa agtcgtcccg gtcgtcaacg agaacgacac ggtcgtgacc gacgagatcc
      781 ggttcggcga caacgacaag ctcgccgccc tcgtcgccca cctcctgcgc gccgacctgc
      841 tggtgctgct ctccgacgtc gacgctttgt ataccgcgcc gcccgggcgg ccgggctcgc
      901 ggcggctcga cgacgtgcac gctgaagccg atctcgccgg cgtcgacctc ggcggcgtcg
      961 ggcgagtcgg catcggatcc ggcggcatga tcacgaaggt ggacgccgcc cggttggcga
     1021 cgtcggccgg cattccggta gtcgttgcgt cagctgccgc cgtcgtcgac gcactcgccg
     1081 gaaagcgcgc aggcacgctg tttcatccca cgggcgcgcg ggttgctagt cggctgttgt
     1141 ggctggctca tgcgacaacg ccacgcggtc ggctggtgct cgacccaggc gccgtcgcgg
     1201 cggtggtcga ccggcggttg tcgttgttgc ctgccggcat cacgtccgtc gaaggcgact
     1261 tcgccgccgg cgacccggtc gacctggtcg acgaggcggg ccgtgctgtc gcccgcggcc
     1321 tcgtcaacta cgacgcgacc gagttgcccg gactgctcgg gcgttcgacc cgcgacctgg
     1381 ctgccgaact cggcacggcg tacgaacgtg aagtcgtgca tcgcgacgac ctggtgctgc
     1441 tcacgaatcg ctgagtgtca atccgcggca gtcgacgccg ccgcctgcag tacctatcct
     1501 cgccttacac ggcgcacgtg tgcgaggagg cagtggggcg tgacgacctg ccagcactgc
     1561 ggcgccgaga tgcccggtga cctgaagttc tgccgcgaat gcggcagttc gatggcctca
     1621 gtgcggtcgt gctcatcgtg cggtgccccc gacgagggcg gccgcttctg cggcaactgc
     1681 ggagcgacgc tgaagccagg gctgataccc gccgcgcggg acaacccggt cgccgagcga
     1741 cgcgtcacgt cggtgctctt cggtgatctg gccggcttca ctccgctgtc ggaatcacgc
     1801 gacgccgagg atgttcgcga gttgttgtcg cgctatttcg ttgagtgccg cacgatcatc
     1861 ggtcgctacg gcggcatcgt cgagaagttc atcggcgatg cggtgatggc agtctggggc
     1921 gtaccgcttt ctcacgagga cgacgccgag cgggccgtgc gggccgggct tgagctcgtc
     1981 gaatcgacga aggcgctcgg cgagcgcgtc ggcgtgcctg gactcgcgat gcgggtcggg
     2041 gtggtgaccg gcgaggtcgc ggtcaccgtc ggcgcaacgg cggaaggcat ggtcgccggt
     2101 gacgccgtga ataccgcgtc gcgggtgcag tcggtcgcgg agcccggatc ggtctgggtc
     2161 gacgacacga cgcgcgcact caccgcggcc tccgtctctt acatggatgt cggcgagcac
     2221 tggctgaagg gcaaggccga accggcacgt ctctggcagg cgcgcgcggt ggtcgccgac
     2281 ctgggcggcg gccagcgggt tgacggattg gaggcgccgc tggtcggccg cgaacgcgag
     2341 gtccggctca tcaaggagct cttccacgcc acgaatgagt cgcagcgacc gcgcctcgtg
     2401 gtgcttgacg gcgagcctgg agtcggtaag tcgcggcttg cctgggagtt cgagaagtac
     2461 gttgacggcc tcaaggccat aacgcgttgg catcgcggtc gctgcctgtc ctacggtgac
     2521 ggcgtcgcat tctgggcgct cgccgaggcg ctgcgcccgc gcctcgggct gaccgagtcc
     2581 gacgccggcg acgcggtcgc cgcagggttg gaggcgagtc tcgccgagtt cgtttcccgg
     2641 tcggaagagc gcgactggct gcgaccgcgg ctggcggcgc tggtcagcgg cggccaagcc
     2701 ggcgggttcg cacgcgaaga tctttttact gcgtggacag cgtttctgga gcggatcagt
     2761 gaagacggca acaccgttgt cctcgtgatc gaagacgcgc actacgcgga cgacgggttg
     2821 cttgcgttcc ttgagtcgat gctcgcgaac gccaacgcgc cgatcttcgt gcttgcgctc
     2881 gcgcgtcccg aactgttgac ccgccgcccc gaccttggtg gccgccggac cagcgtcgtg
     2941 cgcctcgatc ccctcgatga cgccacgatg tcgacgctgg tcgatggcct tgttgacggg
     3001 ttaccagcgg tcgccaggaa cgcgctcgtg gagcgtgccg aaggcattcc gctctttgct
     3061 atcgagaccg tgcgagcact gatcgaccag gacgcggtgg tcccgcgcga tgggcgttac
     3121 gtgcctgccg acggagtcac gatcgacctt gcctcgatcg gcgccccggc gtccctgcag
     3181 gcgctcgttg ccgctcgcct tgatgcgttg tcgccgacag agcgcgccgt cgttgccgac
     3241 gcgagcgtgc tgggcacatc gtttgcccgc gaagggctgt tggcgctcgg ctccgaccgt
     3301 gacggcctcg acgacgcgct ggcgacgctg cagcgcaagg agatcatcgc cttgcagcag
     3361 gaccggttct cggccgaccg cggccagtac cgcttcgtgc agtcggtcgt ccgccaagtc
     3421 gcgtacggga cgttgtcacg acgcgaccgc aaagcgcggc atctcgcggc ggccgacttc
     3481 ctgtctgccc aactcgacgc gaacgacgac ctcgccgttg tcgttgcgca gcaccttctc
     3541 gatgccgtcg acgcaggcgg cgcgtcagat gacgacacgt cggagttgtt gccgagagcc
     3601 tgtgcccttc tggagcgggc gggcattcgg gccagagcgc tgggctctcc ggccgaggca
     3661 cgccgccgct tcgatgccgc gttggctcgc gccacggatc cagcagcgcg cgccagtctc
     3721 tatctcgcgg ccgccgatgc ggcgtacgac tccggcgact atgagggtgc tgtcgctgaa
     3781 ggtgcggcgg cgaccgattt gtttgacgcg ctgggcaggc caatcgatgc ggcgctggcc
     3841 gcggcgacgg gcagcatggg tctgcagaca cttggcgatt ttgccggcgt catccgcatc
     3901 gccgaaccac gatggcaggc gctagacggc gtcgcgggag ccgagcgggc gttgctccag
     3961 ctagcgaggc cgcttgcgag cgccacaggg cagctcgagg acgaggccgc gagcggacgg
     4021 tacacgcagc ggcgggtgct act
//
LOCUS       DATCBJ010000598         3349 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_274785, whole genome shotgun sequence.
ACCESSION   DATCBJ010000598 DATCBJ010000000
VERSION     DATCBJ010000598.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 3349)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3349)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3349
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_274785"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(136..1197)
                     /locus_tag="VII50_12025"
     CDS             complement(136..1197)
                     /locus_tag="VII50_12025"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009083418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase"
                     /protein_id="HEY5051622.1"
                     /translation="MTPLSGPATADAVLDRVLTADIEEAVAALDALPAGTAFARTRRP
                     AVGGDRKTGLEQFALGFFVVIPFLALLAAIPVFWGWGLGWHDIVIAVVMYAIAGHGIT
                     IGYHRLFTHKSFKANRPLKIALAFAGGMAIEGPVVRWVADHRRHHAFSDKEGDPHSPW
                     RFGTSLGALTKGLLYAHVGWIFDPEQTDATRYAPDLIADPDIVAVSRLFWLQVTTSLL
                     LPALIGGLWSMSIAGAVTGFFWGALVRISLLHHVTWSINSICHAVGERPFRTRDKSGN
                     VWWLSVLSMGESWHNLHHADPTSARHGVLRGQLDSSGRTIWVFEKFGWVHDVRWPSPE
                     RIKARLVRDLERENQTASV"
     gene            1308..1381
                     /locus_tag="VII50_12030"
     tRNA            1308..1381
                     /locus_tag="VII50_12030"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1340..1342,aa:Gln,seq:ttg)
     gene            1483..3018
                     /gene="glmU"
                     /locus_tag="VII50_12035"
     CDS             1483..3018
                     /gene="glmU"
                     /locus_tag="VII50_12035"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0006048 - UDP-N-acetylglucosamine
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HEY5051623.1"
                     /translation="MTDTRERIAAAVVLAAGEGTRMKSATPKVLHELCGRSMLGHVIA
                     AMRGADPHALVVVTGQGRDQVEDHLKLLDPEAIAVFQSSQNGTGHAVRIALQALDASN
                     GRPIEGTILVAPGDTPLLSAAAVMALAARLAVTQTAAVVLTANVAEPTGYGRIVRDEA
                     GNLAHIVEHADATDAERELAEVNSSVYAFDAAALREALGQLTAHNAQAEEYLPDVVRI
                     LVRSDHPVGAVLIDDWREIAGVNDRVQLAQARAYLRDRLLTTFMRDGVTVVDPATTWI
                     DVDVVLEPDAVVLPNTTLLGRTTIASGARVGPNCSLLDTAVGAGAVVRDATCEGADIG
                     PLATVGPYTYLRPGTRLARGAKAGGFVEMKNAVVGADSKVPHLSYVGDATIGERSNIG
                     AATIFANYDGEEKHATVVGDDVRIGSDTMLVAPVTIGDGAYTAAGSVIVEDVPPGALA
                     VGRGRQRNVEGWVEKKRPGSASARSAARSAASGPDLPGADLPNGPSTHDNGSPDESQG
                     DHS"
     gene            3051..>3349
                     /locus_tag="VII50_12040"
     CDS             3051..>3349
                     /locus_tag="VII50_12040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003405263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase-like
                     domain-containing protein"
                     /protein_id="HEY5051624.1"
                     /translation="MLFAGRAFPELALEVAHHLNIEPTPTLAYEFANGEIFVRFEESV
                     RGCDAFVLQSHTAPINKWIMEQLIMVDALKRASAERITVVAPFYGYARQDKKHRGR"
BASE COUNT          572 a         1114 c         1152 g          511 t
ORIGIN      
        1 gcggccctgg tcagcgcccg gctcgagtgt caggtccagg gtgcttcggg tgcaggcgac
       61 gtgtgggtgc tggagatgcc tgaaggctgg cgcgttcgac ttccgtgctc agtcgtcgat
      121 cgctgaccgc gacgcctaga ccgaggcggt ctggttctcg cgctcgagat cgcgcacgag
      181 gcgcgccttg atgcgctcgg gcgatggcca gcggacgtcg tgcacccaac cgaacttctc
      241 aaacacccag atggtgcgtc cgctcgagtc gagttggccg cgcagcacac cgtgtcgcgc
      301 ggacgtcggg tcggcgtggt gcaggttgtg ccacgactcg cccatcgaca gcaccgagag
      361 ccaccacacg ttgccggact tgtcacgcgt gcgaaacggc cgctcgccca ccgcgtggca
      421 gatcgagttg atcgaccagg tgacgtgatg cagcaacgag atgcgaacca gtgcgcccca
      481 gaagaagccg gtgaccgccc cggcgatcga catgctccag agtccgccga tgagtgcggg
      541 caacagcagg gacgtcgtga cctgcagcca gaacaaccgc gacaccgcga cgatgtcggg
      601 gtccgcgatc aggtcaggtg cgtaccgggt cgcatccgtt tgctccgggt cgaagatcca
      661 accgacgtgc gcgtagagca ggcccttggt cagcgcgccg agacttgtgc cgaaccgcca
      721 cggcgaatgc gggtcaccct ctttgtcgct gaaggcgtgg tggcggcggt ggtcggcgac
      781 ccagcggacg acgggccctt cgatggccat gccgccggcg aacgcgagcg cgatcttcag
      841 cggccggttg gccttgaacg acttgtgcgt gaagagccgg tggtagccga tcgtgatgcc
      901 gtggccggcg atcgcgtaca tgacgaccgc aatgacgatg tcgtgccagc ccagtcccca
      961 gccccagaac accgggatcg cggcgagcag cgccaagaac gggatcacga cgaagaagcc
     1021 gagcgcgaac tgctcgagac cggtcttgcg atcgccgccc accgcgggcc gcctggtccg
     1081 tgcgaacgca gtcccagcag gcagcgcgtc gagcgccgca acggcttcct ctatatccgc
     1141 tgtcaaaacc cgatcgagga cggcgtcagc cgttgcgggt ccggacagcg gggtcatggc
     1201 ggctccagaa acggctcggg gggtaaggcg atgctaaacc acgctgcggg cgttccccgc
     1261 atatcggcct gcgggcgctg gttgcgtact ctgagcgcgg acaccgatcc cgggtcgtct
     1321 aatggcagga cagtgggttt tggtcccatc aatgggggtc cgactcctcc cccgggagcc
     1381 acgtgtcgcc gatcccatct ccccggcagt ccgctgggga gatgggagac ggtgtagccc
     1441 agaactacgg tgtcgcccaa ggagttcagc cgaaggagcc gcgtgaccga cacacgggag
     1501 cgaatagcgg cagccgtcgt ccttgcggcc ggcgagggca cccggatgaa gtcggcgacg
     1561 ccgaaggtgc tgcacgaact gtgcggtcgc agcatgctcg gccacgtcat cgctgccatg
     1621 cgcggcgccg acccgcacgc gctcgtcgtc gtcacgggtc aaggtcgcga ccaggtcgag
     1681 gatcacctca agcttctcga ccccgaggcg atcgcggttt tccagtccag tcaaaacggc
     1741 accggacacg ccgtccgcat tgccttgcaa gcccttgatg ccagcaacgg ccgcccgatc
     1801 gagggcacga tcctcgtcgc gcccggcgat accccgcttc tgtcagccgc cgccgtgatg
     1861 gcgctcgcgg cccggctcgc ggtgacccaa acggccgctg tggtgctgac ggccaacgtc
     1921 gctgagccca ccggttacgg ccggatcgtg cgcgatgagg cgggcaacct cgcacacatc
     1981 gtcgagcacg ccgatgcgac cgacgccgaa cgcgagctag ccgaggtcaa ttcgagcgtc
     2041 tacgccttcg acgccgcggc gctgcgcgag gcgctcggac aattgaccgc ccacaatgcc
     2101 caggccgagg agtacctgcc cgacgtcgtc cgcattctgg tgcgcagcga tcacccggtc
     2161 ggcgctgtgc tgatcgacga ctggcgcgag atcgcgggag tcaacgatcg cgtgcagctc
     2221 gcgcaagcca gggcatacct gcgcgatcgc ctgctcacga cattcatgcg ggacggcgtg
     2281 acggtggtcg atccggcgac gacctggatc gacgtcgacg tggttctcga accggacgcc
     2341 gtcgtgctgc cgaacacgac gttgctcggg cgcaccacga tcgccagcgg cgcgcgggtc
     2401 gggccgaact gctcgctgct cgatacggca gttggtgcgg gcgcggtggt ccgcgatgcc
     2461 acgtgcgagg gcgccgacat cgggccgctc gcgaccgtcg gtccctatac ctatctgcgc
     2521 ccgggcacgc ggctcgcgcg cggcgccaag gccggtggct tcgtcgagat gaagaacgcc
     2581 gtcgtcggtg cggattcgaa ggtcccgcac ctctcttatg taggggacgc gacgatcggg
     2641 gagcggagca acatcggcgc cgcgacgatc ttcgccaact acgacgggga ggaaaagcat
     2701 gcgacggtcg tcggggacga cgtccggatc ggcagcgaca cgatgctggt cgcacccgtg
     2761 accatagggg acggcgcgta caccgcggcc gggtcggtga tcgtcgaaga cgtcccgccc
     2821 ggggcgctcg ccgtgggtcg tggacgccag cgcaacgtcg agggttgggt cgaaaagaaa
     2881 cggcctgggt cggcgtccgc ccgttccgct gctcgatctg ccgcatcggg gccggacctt
     2941 ccgggggccg acttacccaa cgggccgtcg acccacgaca atggttcccc ggacgaatcc
     3001 cagggagacc actcgtgacc ggtatcaagc ggaccggcga gaagaccctc atgctcttcg
     3061 ctgggcgggc gttccccgag ctcgcgctcg aggtggccca tcacctcaac atcgagccga
     3121 cgccgacctt ggcctatgag ttcgcgaacg gcgagatctt cgtccgattc gaggagtcgg
     3181 tgcgcgggtg cgacgcgttc gtcctgcaga gccacaccgc gccgatcaac aagtggatca
     3241 tggaacagct gatcatggtt gacgcgctga agcgggcgtc cgccgagcgc atcaccgtgg
     3301 tcgcgccgtt ctacggttac gcgcgccaag acaagaagca ccgtggtcg
//
LOCUS       DATCBJ010000599         3360 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_268298, whole genome shotgun sequence.
ACCESSION   DATCBJ010000599 DATCBJ010000000
VERSION     DATCBJ010000599.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 3360)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3360)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3360
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_268298"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..665)
                     /locus_tag="VII50_12045"
     CDS             complement(<1..665)
                     /locus_tag="VII50_12045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY5051625.1"
                     /translation="MTATKRAGAEPGIAYVVSGLSKVYRAKSGDVTATDDIALEVRRG
                     EVFGLLGPNGAGKTSLVRQLIGLLRPDAGRIQLFGRDVVAVPRVVPDFVAYLPQDDAA
                     LADLTTAGALETTARLRGLSRPGATAARDDVIDELGLGGFADRPIAKLSGGQRRLAGV
                     GAALVGDRPVLVLDEPTTGLDPSARRAVWSALERRRSEQATTVVLVTHNVLEAETVLD
                     RVA"
     gene            complement(701..1663)
                     /locus_tag="VII50_12050"
     CDS             complement(701..1663)
                     /locus_tag="VII50_12050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5051626.1"
                     /translation="MTTPAFRAILPASGDQDLVAEGGVSMAWSSRRAVLAAMVGASVA
                     ASVVTFAGNDAHAAAPPPRPSVANTGVPPGTVLTEHDGNITITAAGTTLKDLDIHGFV
                     VVEASNVTIEDSVVRGGVSHGDTALVKVVSGTNVLVEDSELVPEYTSVQIDGIRGANY
                     TALRLNIHGTNDASKITGDNVTIEDSYIHDLPFFPHDPDQGGNPAHNDGVEIFVGKNL
                     HITDNAFYIATTGNSAMQVNQNLGVVTDLHFTGNYADGGHCTVNLAIQPRPSMTGIQV
                     DNNRFGRDTTVVNCAIFAKYGVTLTDVNDVWDDTGLPVTLRRAH"
     gene            complement(1832..>3360)
                     /gene="dnaE"
                     /locus_tag="VII50_12055"
     CDS             complement(1832..>3360)
                     /gene="dnaE"
                     /locus_tag="VII50_12055"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HEY5051627.1"
                     /translation="DYADRKNGRKPVVAIHPELAEPLDEILGETHGLLVYQEQVLAIA
                     QKVAGYSLGQADLLRKAMGKKKKEILDKEYDFFSAGMRERGYSPGAIKALWDTLVPFS
                     DYGFNKAHSAGYGVVSYWTAYLKANYPAEYMAALLTSVKDDKDKSALYLHECRRMGIK
                     VLPPDVNESDSNFTPRGTDIRFGLSAIRNVGENVVGSIIETRRAKGRYKDFVDFLAKV
                     EPVVCNKKVVESLIKAGSFDSLGHTRRGLVAVHAEIIDAAMGTKRAEAIGQFDLFGSA
                     DEDQGPSGLGLAFEIAIPSTEWDKTALLAAERDMLGLYVSDHPLFGIEHVLSRLVDCS
                     ISSLTTDEHPEGGIITIGGIVSGLQRKMTKQGNPWAIATVEDLEGSIEVLFFPQTYQQ
                     YAMSLSEDAVICVKGRLSHRDEGLNLQAIELFLPDVGEAAQRGPVVLQLPAARCTPPV
                     VERLREVLATHPGVTEVQLQLRNGARTTVLRLDENLRVDATPALMGDLKALLGPSCLV
                     "
BASE COUNT          597 a         1067 c         1089 g          607 t
ORIGIN      
        1 atcgcgaccc ggtcgaggac cgtctcggcc tcgagcacgt tgtgggtcac cagcaccacg
       61 gtggtcgcct gctcggagcg gcggcgctcg agcgcgctcc acactgcacg gcgcgcgctc
      121 ggatcgagac cggtcgtcgg ctcgtccaga accagcaccg gccgatcccc gaccaaggcc
      181 gcgccaacac cggccagcct gcgctgacct cccgaaagct tggcaatagg ccgatccgcg
      241 aacccaccga gtccgagctc gtcgatcacg tcgtcgcgcg ccgccgtcgc gcccgggcgg
      301 gacagcccgc gcaaccgggc ggtggtttca agggcgccgg cggtcgtcag gtccgcgagt
      361 gcggcgtcgt cctgcggcaa gtaggcaacg aaatccggga cgacgcgggg taccgcaacg
      421 acgtcacggc cgaacagttg gatgcggcca gcgtccggac gcagcaaccc aatgagctgc
      481 cgcaccaagc tcgtcttgcc cgcgccgttg ggaccgagca ggccgaagac ctcgcctcga
      541 cggacctcaa gggcaatgtc gtcagtcgcc gtgacgtcac ccgactttgc ccggtagacc
      601 ttcgagagtc cgctgacgac gtaggcaatt cccggctcgg cacccgcacg cttggtggcc
      661 gtcatctcgc ggacagccta accgccccgt aggcggccga tcagtgggcg cggcgaagcg
      721 tgacgggcaa gcccgtgtca tcccagacgt cgttgacgtc ggtgagggtc accccgtact
      781 tcgcaaagat tgcgcaattc acgacggtcg tgtcgcggcc gaatctgttg ttgtcgactt
      841 gaatacccgt catcgacggt ctcggctgga ttgcgagatt cactgtgcaa tggccgccgt
      901 cggcgtaatt gccggtgaaa tgaaggtcgg taacgacacc gagattctgg ttaacctgca
      961 tggccgagtt gccggtggtc gcgatgtaga aagcattgtc ggtgatgtgc aggttcttgc
     1021 cgacgaagat ctcgacgcca tcgttgtgcg ccggattccc accctggtcg ggatcatgcg
     1081 gaaagaaggg aaggtcgtgg atataggagt cctcaatcgt gacgttgtcg ccggtgatct
     1141 tcgaagcatc gtttgtgccg tggatgttga ggcggagggc cgtgtagttg gctccgcgga
     1201 tgccgtcgat ctgcacggac gtgtattcgg ggacgagttc gctgtcttcc accaatacgt
     1261 tggtgcctga cacgaccttc accagggcgg tatcgccgtg cgaaacaccg ccgcgcacga
     1321 ccgaatcctc gatcgtcacg tttgacgcct cgacgacgac gaacccgtgg atatcgagat
     1381 ccttcagggt cgtgccggct gcggtgatcg tgatgttgcc gtcgtgttcg gtcaacaccg
     1441 tgccgggcgg gacgccggtg ttcgcaaccg agggccgcgg cggcggcgcg gccgcgtgag
     1501 cgtcattgcc ggcgaaggtc acaacgctag cggccaccga ggcgccgacc atggcagcga
     1561 gcacggcccg ccgcgaactc cacgccatcg agactccgcc ctcggcgacg aggtcttgat
     1621 cgccggatgc agggagtatg gccctaaatg caggcgttgt caccacgcct gcgacaccat
     1681 cgcagcacga tgatcgggcc cagcacggtc tcgttcgcta gaagccgcgc cgtcgacggc
     1741 gcaggaagcg ggctatcgca agggtgagcg aggagaagat cgaacgacgg ccgacggttg
     1801 acatgtgacc atcatggccc tgtgagtgcg gctagacgag acaggatggg ccgagtaacg
     1861 ccttgagatc gcccatcagg gcgggtgtcg catcaacccg caggttttcg tcgagccgca
     1921 gcaccgtcgt ccgcgcgccg ttgcgcagct gcagctgcac ctcggtgaca cccggatgcg
     1981 tcgccagcac ctcgcgcaag cgctcgacca cgggcggcgt gcacctggct gcgggcagct
     2041 gcaacaccac gggcccgcgc tgggcggctt caccgacgtc gggcaagaac agctcgatcg
     2101 cctgcaggtt caacccctcg tcacggtggc tcaaccgacc cttgacgcag atgacggcat
     2161 cttcggacag cgacatggcg tactgctggt aggtctgggg gaagaacagc acctcgatcg
     2221 acccttcgag atcctcgacg gtcgcgatcg cccacggatt gccctgcttg gtcatcttgc
     2281 gttgcaaccc ggagacgatg ccaccgatcg tgatgatgcc gccttcggga tgttcgtcgg
     2341 tggtcagtga actgatcgag cagtcgacga ggcgtgagag aacgtgctcg ataccgaaca
     2401 gcggatggtc agagacgtag aggccgagca tgtcgcgctc ggcggcgagc aacgcggtct
     2461 tgtcccactc agtcgagggg atcgcgatct cgaatgcgag gccgagacca cttggtcctt
     2521 ggtcctcgtc cgccgacccg aacaagtcga actgaccgat cgcctcggct cgcttggtgc
     2581 ccatcgccgc atcgatgatc tccgcgtgta ccgcgacgag gcctcgtcgg gtatgcccga
     2641 gcgagtcgaa cgaaccggct ttgatcagtg actcgacgac cttcttgttg cacacaaccg
     2701 gctcgacctt cgcaaggaag tcgacgaagt ccttgtagcg gcccttggcg cgacgcgtct
     2761 cgataatcga tccgacgacg ttctccccga cgttgcggat tgccgacaga ccgaacctga
     2821 tgtcggttcc gcgtggggtg aagttggagt cagactcgtt gacgtcgggc ggcagcacct
     2881 tgatgcccat gcgcctgcac tcgtgcaggt agagcgccga cttgtctttg tcgtccttga
     2941 cgctggtcag cagtgcggcc atgtactcgg cgggatagtt cgccttgaga tacgcggtcc
     3001 agtacgacac gacgccatag cccgcgctgt gcgctttgtt gaatccgtag tcggagaacg
     3061 gcacgagggt gtcccacagc gccttgatcg cacccgggct gtaaccgcgc tcgcgcatgc
     3121 cagcactgaa gaagtcgtac tccttgtcga ggatctcctt cttcttcttg cccatcgcct
     3181 tacggagcaa gtcggcctgg ccgagcgaat agcccgcgac tttctgcgcg atcgcgagca
     3241 cttgttcctg gtacacgagc agaccgtggg tctcgccgag gatctcgtca agcggctcgg
     3301 cgagctctgg gtggatcgcg acaacgggct tgcgaccgtt cttgcgatcc gcgtagtcat
//
LOCUS       DATCBJ010000600         2173 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_386505, whole genome shotgun sequence.
ACCESSION   DATCBJ010000600 DATCBJ010000000
VERSION     DATCBJ010000600.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2173)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2173)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2173
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_386505"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1..320)
                     /locus_tag="VII50_12060"
                     /pseudo
     CDS             complement(1..320)
                     /locus_tag="VII50_12060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611158.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            581..1798
                     /locus_tag="VII50_12065"
     CDS             581..1798
                     /locus_tag="VII50_12065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012226942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HEY5051628.1"
                     /translation="MTDNKIALRELLEKGSDATFLREVIGFAAERLMALETEGLCGAA
                     PGERSVERINQRNGYRERDWHTRAGTVELRIPKLRHGSYFPGFLEPRRMAEKALTAVI
                     QEAYIQGISTRSVDDLVKALGMEGISKSQVSRLCGEIDERVHAFLARPIEGDWPYVWL
                     DATYVKVRRDHHIVSVAVIIAVGVNTDGRREVLGMTVGHSEAEPFWVEFLRSLTRRGL
                     RGTKLVISDAHEGLRSALSQVFVGATWQRCKVHFLRNVASAVPKLHAPAVLVVVKSIF
                     LQPTRETARDAVNHALTVLEPRFPTVAAKLRAAENDVLAYLDFPVDHWRSISSTNAIE
                     RVNAELDRRAKVVGIFPNTASLLRLFTAVLQDQHDEWQDGRRHFSQQSMARVTHPDGP
                     PLLTNPLTEGLVA"
     gene            complement(1869..1942)
                     /locus_tag="VII50_12070"
     tRNA            complement(1869..1942)
                     /locus_tag="VII50_12070"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1906..1908),aa:Pro,seq:ggg)
     gene            complement(1993..>2173)
                     /locus_tag="VII50_12075"
     CDS             complement(1993..>2173)
                     /locus_tag="VII50_12075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HEY5051629.1"
                     /translation="ILYATQARSPVPTIVLFVNDPELMHFAFQRYLENRIRAVFGFAG
                     VRLRIVTRHRDESDD"
BASE COUNT          386 a          740 c          678 g          369 t
ORIGIN      
        1 ggcacctccg caaagatgcc gacggccttt tttaacacgt cgcgctccat gcgcacgcgg
       61 tcgagttcac gtttgagctt catgttctgc gacgccaggt ccgcaggcga tgtgccgggc
      121 ggtgcaatcc ctccatcccc gtttgcccgc ggccgcgatc gtgggggccc gccttgaatg
      181 attgtccgcc aggcccgcag catcgagggc tggatgccca actccaccgc aacctgcgtc
      241 aatggccggc cgctgctttc aagcagggcc actgcctcgc gcttgaactc gggcgcgaac
      301 tcccgcctcg tcttgatcat cggccacctt tcagctccca aaggagcgta tcaaaggtgt
      361 ccaccaaagt gggggaggat caagcctacc tcgcggtcgg tctctcagcc gatagcggtc
      421 acgtcccggg tcgtggtgta ggagctgtgg gtaagtagcc cgccgtagcg accggtgaga
      481 gtctggcgcg ggcgggctac ttatccacag cggtgatcac cgcggcggtg ctgggtaggg
      541 tcgggttgca acacgcaact ctagcccgag gaccaccacg atgaccgaca acaagatcgc
      601 ccttcgcgag cttctggaga agggctccga cgcgacattc ctgcgtgagg tgatcggctt
      661 tgccgccgaa cgcctgatgg cgctggagac cgagggactg tgcggggccg cacccggcga
      721 gcgcagcgtc gaacggatca atcagcgcaa cggctaccgc gagcgggact ggcacacgcg
      781 agccggcacc gtcgaactgc gcatccccaa gctgcgccat gggagctact tcccaggctt
      841 tctggagccg cgccggatgg ccgagaaagc tctgacggcg gtgatccagg aggcctacat
      901 ccaaggcatc tccacccggt cggtcgacga tctggtgaag gcgctcggca tggagggcat
      961 cagcaagagc caggtctccc gcctgtgcgg cgagatcgac gagcgcgtgc acgccttcct
     1021 ggcacggccg atcgagggcg actggccgta tgtctggctc gatgccacct acgtcaaagt
     1081 ccggcgtgac caccacatcg tctcggttgc cgtcatcatc gccgtgggcg tcaacaccga
     1141 cggtcggcgt gaggtgctgg gcatgacggt cggccacagc gaggccgaac cgttctgggt
     1201 cgagtttctg cgcagcctga cgcgccgcgg cctgcgcggc accaagctgg tcatctccga
     1261 cgctcatgag ggattgcgca gtgccttgtc gcaggtcttc gtcggcgcca cctggcagcg
     1321 ctgcaaggtg cacttcctgc gcaacgtggc cagcgccgtt cccaagctgc acgcgccggc
     1381 ggtgctcgtg gtcgtgaagt cgatcttcct gcagccgacg cgcgagactg cccgcgacgc
     1441 cgtcaaccat gcactcaccg tgttggagcc acgcttcccc acggtggcgg ccaagctgcg
     1501 cgctgccgag aacgacgtgc tcgcctatct ggatttcccc gtcgaccact ggcgctcgat
     1561 ctcgtcgacc aacgccatcg agcgcgtcaa cgccgagctc gaccggcgcg ccaaggtggt
     1621 gggcatcttt cccaacaccg cctcactgct ccgcttgttc accgcggtgc tccaggacca
     1681 gcacgacgaa tggcaggacg gacggcgtca tttcagtcag caatcgatgg ctcgggtgac
     1741 gcaccccgac ggtcccccgt tgctcaccaa cccgctcacg gagggactcg tcgcttaatt
     1801 caccgacgtt gtgagaactc caccgaattc acaccactac cagggacttg accgcaatgg
     1861 gctgcgaatc ggggagacag gactcgaacc tgcgacccct ggtcccccag accagtactc
     1921 taaccatctg agctactccc cgaggcgcgc ctagcgactc taccgcccca ggcccacgac
     1981 gccgaaaacg cctcagtcgt cgctctcgtc gcggtgccgg gtgacgatcc gcaatcgcac
     2041 ccccgcgaac ccgaacaccg cgcgaatccg gttctcgagg taccgctgga atgcgaagtg
     2101 catcagctcg gggtcgttaa cgaagagcac aatcgtaggc acggggctgc gagcctgggt
     2161 cgcgtagagg atc
//
LOCUS       DATCBJ010000601         3073 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_422948, whole genome shotgun sequence.
ACCESSION   DATCBJ010000601 DATCBJ010000000
VERSION     DATCBJ010000601.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 3073)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3073)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3073
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_422948"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..424
                     /locus_tag="VII50_12080"
     CDS             <1..424
                     /locus_tag="VII50_12080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009071995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HEY5051630.1"
                     /translation="AGTLSRNPLATAAGLATLRACTRQVYAHLDATAAQVGALASAAL
                     TQAGVAHRLQYAGNLFSLFFTDREVVNFDDARAQESWRYAAFFHALLDRGVYAPPSAF
                     EAWFVSAAHDETAIERIAAALPDAARAAASSVRPEVGT"
     gene            421..1056
                     /locus_tag="VII50_12085"
     CDS             421..1056
                     /locus_tag="VII50_12085"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEY5051631.1"
                     /translation="MSERTVVHLLRHGEVNNPDHVLYGRLPGFHLTDAGQAMAKRAAV
                     AVADRDIVAVISSPLERAIETATPVAEQFDLAIAVDERLIEATNVFEGLTFGVGDGSL
                     RRPAHWRYLTNPFRPSWGEPYRELAVRVLAAVASARDAARGHEAICVSHQLPIWTARR
                     EVAGKHLWHDPRRRQCALGSLTSFTFDGDTIVAVDYTEPSGRAGIGQVAGA"
     gene            1068..1643
                     /locus_tag="VII50_12090"
     CDS             1068..1643
                     /locus_tag="VII50_12090"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HEY5051632.1"
                     /translation="MIAAPTALAIVLGLSGCSTSASATNDQQTNYVALNGVGVLTVYA
                     VGHRKLAPPVSGTDLGGHPLTLSQFAGKVIVLNFWASWCPPCRGEAPALEQVYTDTRA
                     SGVQFVGVDIRENGVSDGAHFETTHHIDYPSFSDQASRIALDFRSTGVETPPTTIVID
                     RQGRIAARGLGEMTYPQLLSAVQSVAAQAST"
     gene            1643..2416
                     /locus_tag="VII50_12095"
     CDS             1643..2416
                     /locus_tag="VII50_12095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcdA"
                     /protein_id="HEY5051633.1"
                     /translation="MAVVASVGSSFQHTVLTGPLLLALPLALIAGVVSFLSPCVVPLV
                     PGYLSYITGLSGADIDDSRGGQRGRVLAGSLLFVLGFSVVFVISGALFGYAGATVRAH
                     TVTIDRILGVVIIALGLVFMGLVPGLQREARFHRVPAAGIAGAPILGFMFGVGWTPCI
                     GPTLGAVQSLAFQGSSATQGALLTFVYCLGLGLPFVVTALAFRRAIGVLGVVKRHYAL
                     VTRIGGVMLIAVGVLLVTGAWHTLVGDLQSTITGYVPAV"
     gene            2418..>3073
                     /locus_tag="VII50_12100"
     CDS             2418..>3073
                     /locus_tag="VII50_12100"
                     /inference="COORDINATES: protein motif:HMM:NF016995.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein ResB"
                     /protein_id="HEY5051634.1"
                     /translation="MKEIPTDVDDVAAAAKLDTAPVDREEPVEQDELGTRQRGGRSTA
                     TSVLGWLRWSWYSLTSMRTALILLFLLALAAVPGSVLPQHASNPLRVRQYYVAHPTLA
                     PIINAIGGFDVFGSPWFAAVYLLLFISLAGCVIPRSRRHIRAMRARPPVAPRNLGRLP
                     YFTSWTTAVPASDVVAASYGLLRSKRFRSDAASTSVAAEKGHLRETGNLVFHLSLLVV
                     "
BASE COUNT          447 a          990 c         1013 g          623 t
ORIGIN      
        1 agccggcacc ctgtcgagaa atccgctcgc caccgctgcg gggctcgcga cgctgcgcgc
       61 gtgcacgcga caggtctatg cgcatctcga cgcgacagca gctcaggttg gtgcgctggc
      121 gtcagcggcc cttacgcagg ccggggtcgc gcaccgcctg cagtacgcgg gcaacctgtt
      181 ctcgttgttc ttcaccgatc gcgaggtcgt gaacttcgat gatgcgcgag cgcaagagtc
      241 ctggcgctac gccgccttct tccatgcgtt gctcgaccgc ggtgtctacg ccccgccgtc
      301 cgccttcgaa gcgtggttcg tgtcggcagc ccatgatgag accgcgatcg agcggatcgc
      361 cgcggcgttg ccggacgccg cgcgagccgc tgccagcagc gtccgaccgg aggtcggcac
      421 atgagcgagc gcaccgtcgt ccatctgttg cggcatggcg aggtgaacaa ccccgatcac
      481 gttctctatg ggcgcctgcc gggttttcac ctgaccgacg ccggccaggc gatggcaaaa
      541 cgggccgcgg tcgctgtcgc cgaccgcgac atcgtggcgg tgatctcgtc cccgctggag
      601 cgggcgatcg aaaccgcgac accggtggcc gaacagtttg acctcgccat tgccgttgac
      661 gaacgactga tcgaggccac caacgtcttt gagggtctga cgttcggcgt cggtgacggg
      721 tcgcttcgcc gtcccgcgca ttggcggtat ttgaccaacc cctttcggcc gtcgtggggg
      781 gagccttatc gcgaactcgc ggtgcgggta ctcgcagcag tggcgagcgc gcgtgatgct
      841 gcccgcggtc acgaagcgat ctgcgtcagc caccagttgc cgatctggac ggcgcgtcga
      901 gaggtggccg gtaagcacct ctggcacgac ccgcgtcgcc ggcagtgcgc acttggcagt
      961 ctgacgagtt tcaccttcga tggcgacacg atcgtggccg ttgactacac cgagccatcg
     1021 ggccgcgcgg gtatcggaca ggtcgcgggc gcatgagggg cttccgcatg atcgccgcgc
     1081 ccaccgcgct tgccatcgtg ctcggcctca gtgggtgttc gacgtcggcc tcggcgacca
     1141 acgatcagca gaccaattac gtcgcgctca acggcgtcgg cgtgctcacg gtctacgcag
     1201 tcgggcatcg caagctggcg cccccggtct cgggcaccga cctaggcggt cacccactga
     1261 cgctgtcgca gtttgccggc aaggtgatcg tgttgaactt ctgggcgtcg tggtgtccac
     1321 catgtcgcgg cgaggcgccc gcactcgagc aggtgtacac cgacactcga gcatcggggg
     1381 tgcagttcgt tggcgtcgac atcagggaga acggcgtctc tgatggcgcc cacttcgaga
     1441 cgactcacca catcgactat ccgagcttct ccgaccaggc atcgcgtatc gcgctggact
     1501 ttcgtagcac tggtgtcgaa accccgccga cgacgatcgt gatcgatcgg cagggccgaa
     1561 tcgctgctcg aggactcggc gagatgacct atccgcagtt actctccgcg gtgcaatcgg
     1621 tggcagcgca ggcgtcaacg tgatggcggt agtcgcatcg gtgggcagct cctttcagca
     1681 cacggtgttg accggtccgc tgttgttggc gctgcccttg gccctgatcg ctggcgtggt
     1741 gtccttcctg tcgccctgtg tcgtcccgct cgtgcccggc tacctctcct acatcacggg
     1801 gctgtccggt gcggacatcg atgattcgcg gggcggccag cgcggccgcg tgctggcagg
     1861 cagtctgttg ttcgtcttgg gattctccgt cgtcttcgtg atctccggag ctctgttcgg
     1921 atacgcagga gcaactgtcc gggcccacac ggtgacgatc gatcgaatcc tcggcgttgt
     1981 gatcatcgct ctcgggctcg tcttcatggg gctcgttccc gggctgcagc gtgaggcgag
     2041 atttcatcgt gtgccggccg cggggatcgc cggcgccccc atcctcgggt tcatgttcgg
     2101 agttggctgg accccatgca tagggccgac gttgggtgca gttcagagtc tggcgtttca
     2161 gggatctagt gccacgcagg gcgccctgct cacgtttgtg tattgccttg gactcggatt
     2221 gcccttcgtt gtcacggcgc tagcgtttcg ccgcgcgatc ggggtgctcg gcgtcgttaa
     2281 gcggcactac gcgttagtca ccagaatcgg cggcgtgatg ttgatcgctg tcggtgtgct
     2341 actcgttacc ggcgcatggc acacgctggt cggcgacctg cagtcgacga tcacgggcta
     2401 cgtgccggcg gtatgagatg aaagaaattc ccacggacgt cgatgatgtc gcggccgcgg
     2461 cgaaactcga cacggcgccg gttgaccgcg aggaacctgt cgagcaagac gagctcggca
     2521 cccgtcaacg aggtggacgt tcaactgcga cgtcggtact tggctggttg cggtggtctt
     2581 ggtactcgct cacatcgatg cggacggcgc tgatcctgct gttcctgctc gcactcgccg
     2641 ctgtaccggg cagcgttctc ccacagcatg cgagcaaccc gctgcgggtg cgtcagtact
     2701 acgtcgcgca cccaacgctc gcgccgatca tcaacgcgat cggtggcttc gacgttttcg
     2761 gctcgccgtg gttcgcggcc gtctacttgt tgctgttcat ttctctcgcg ggttgcgtca
     2821 tcccgcgcag tcgccgccac atacgcgcga tgcgtgcacg gccgcctgtt gcgccgcgca
     2881 accttggccg attgccgtat ttcacgagtt ggacgacggc agtgccggcc agcgacgtcg
     2941 ttgccgcgtc gtacgggttg cttcgttcaa agcgctttcg gtccgacgct gcatcgacga
     3001 gcgtggctgc ggagaagggg cacctgcgcg agaccggcaa cctggtcttc catctctcgc
     3061 tgctcgtggt gtt
//
LOCUS       DATCBJ010000602         3406 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_15633, whole genome shotgun sequence.
ACCESSION   DATCBJ010000602 DATCBJ010000000
VERSION     DATCBJ010000602.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 3406)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3406)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3406
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_15633"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..645)
                     /locus_tag="VII50_12105"
     CDS             complement(<1..645)
                     /locus_tag="VII50_12105"
                     /inference="COORDINATES: protein motif:HMM:NF015923.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquitin-like domain-containing protein"
                     /protein_id="HEY5051635.1"
                     /translation="MRSRLVVGSVCGVITAALVAGTTAFVSLDKNITISVDGQVTKVR
                     TYAASVDGVLHRAGITVGPHDSLTPAPSVKVHDGTTITIARGRLLTLTIDGVTHQAWV
                     TGDNVDEVLQQAGLRIPQAADVSVDRGARVPLTGMNISINLPHLVNVRVDGTVRPVVS
                     TQTTLAGVLAEAGITVNPADQISTDLQTRPFDGLYVIILRISSGQELQSSQIPFT"
     gene            complement(936..1742)
                     /locus_tag="VII50_12110"
     CDS             complement(936..1742)
                     /locus_tag="VII50_12110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HEY5051636.1"
                     /translation="MIAGSPPPAPQPLSVETVDSHCHLDAMELPVAEVVAAAQAVGVR
                     RMVTVGDTMASSRWCAATADAFADVYAAVAVHPNEVNDFTDADADELAQLAALPDVRA
                     VGETGLDYYWGRVGPLVQQAAFRTHIEVAKRAGKALVIHDRDAHDDVLRILAEVGAPD
                     RTVFHCFSGDVSMSRHCVERGYYMSFAGTVTFKNAGALREAAAVVPLELMLVETDAPF
                     LAPVPYRGRPNAPYLIPLTVRALAAVKGVDENAVAAATAANAEHVFGAWR"
     gene            complement(1739..>3406)
                     /gene="metG"
                     /locus_tag="VII50_12115"
     CDS             complement(1739..>3406)
                     /gene="metG"
                     /locus_tag="VII50_12115"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HEY5051637.1"
                     /translation="RNAQRALGSLEGVTDNPGPEAAKAFYLTTPIYYVNDVPHIGHAY
                     TTVAGDVLTRWHRQRGESVWFLTGTDEHGEKVLRTAEAHDVSPQQWADRLVEEEWRPV
                     WRSLDIANDDFIRTTELRHTERVQAFLQGLYDAGEIYEGLYEGPYCVNCEEFKLPGDL
                     LDGTGEYAGQKVCAIHGRPVEELSETNYFFRLSKYGDALLAHYELRPEAVEPESARNE
                     VLSFIRSGLTDLSISRSSFDWGIKVPWDDTQVVYVWFDALLNYATAVGLEAPDAADKE
                     KFAQTWPADVHLVGKDILRFHAVIWPAMLLAAGVALPRKVFAHGWLLVGGEKMSKTKL
                     TGIAPAEITDHFGSDAFRYYFLRAIQFGQDGSFSWEDMSARYTSELANGFGNLASRVT
                     AMTEKYFGGVLPPAEDAGPAEDVVADVASRSVAAADEAVLALDFQGGLAAIWELVGAL
                     NGYLSDQAPWKVAKEIDSSPASRARVATILYTAAEGLRVLAVVLNPFIPRATAALWTA
                     LGAAPALGPLATQNLTDAGRWGQLPVGAAITKGESLFPRLADDAPPA"
BASE COUNT          634 a         1105 c         1112 g          555 t
ORIGIN      
        1 ggtgaaggga atctgcgagc tctgcagctc ttgcccgcta ctgatccgca ggatgatgac
       61 gtacagcccg tcgaacggcc gcgtttgaag gtcggtcgat atctggtcgg cagggttcac
      121 cgtgatgccg gcttctgcga gcacgcccgc caatgtcgtt tgcgtgctga cgaccggacg
      181 cactgttcca tcgacgcgca cattgacgag gtgcggcaag ttgatggaga tgttcattcc
      241 cgtcaaagga acgcgtgcac cacgatcgac agaaacatct gcagcttgcg gaatgcgcaa
      301 ccctgcttgt tgcaacacct cgtcgacgtt gtcaccagtg acccatgctt gatgcgtcac
      361 gccgtcgatc gtcaacgtca acaaacggcc gcgagcgatc gtgatggtcg tgccgtcatg
      421 caccttgact gaaggtgcag gcgtcagtga atcgtggggg ccgacagtga tgccggcgcg
      481 atgcaaaacg ccatcgacgg acgccgcgta ggtgcgcact ttcgtgacct gaccgtcgac
      541 cgaaatggtg atgttcttgt caaggctgac gaacgccgtc gtccctgcta ccagggcagc
      601 agtgatgacg ccgcacaccg aaccgacgac aagcctcgat cgcacgggac tccgctgttc
      661 gaagggtcca caggatggga agcctgggcg cgcaaagcgc aactagatcg cagaaaacga
      721 cccggaaggc cgaccacgag cttccccact ctggccgaac actgccgtga acgtaaccaa
      781 cgagttgttg actaagcaag cgataggcgg cggagatcgc ctgctttggt ctcgatttgc
      841 gctggactta aggaagttcc cggcatttcg acgtgctggc gggcggccgt gtgacgggtg
      901 ggacacgtgt gacgaacggc gaaaccgcgc gggagctacc gccaggcgcc gaagacgtgc
      961 tccgcattgg ctgcggtggc cgccgcgacg gcgttctcgt cgacgccctt caccgcagcg
     1021 agcgcgcgaa cggtgagcgg gatgaggtac ggcgcgttgg ggcgaccgcg atacggcacg
     1081 ggggcgagaa atggcgcgtc ggtctcgacg agcatcaact caagcggaac gaccgcagcc
     1141 gcttcgcgca gagcgccagc gttcttgaac gtgaccgttc cggcgaacga catgtagtag
     1201 ccgcgctcca cacagtgtct tgacatcgag acatcgccgg agaagcaatg gaacaccgtg
     1261 cgatccggcg caccgacctc cgcaaggatg cgcaacacgt cgtcgtgtgc atcgcgatcg
     1321 tgaatgacga gggccttccc ggcgcgtttc gcgacctcga tgtgcgtgcg aaaagcggcc
     1381 tgttgcacga gcggaccaac acgaccccag tagtagtcaa gacccgtctc accgacagcg
     1441 cggacgtcgg gcagcgcagc caattgcgcc agctcgtcgg cgtccgcgtc ggtgaaatca
     1501 ttgacctcgt tggggtgcac cgcgactgcc gcatagacat cggcgaaggc atcggccgtg
     1561 gcagcgcacc agcgcgacga cgccatcgtg tcgccgaccg tgaccatccg gcgcaccccg
     1621 accgcctggg ccgcggcaac cacctcagcg accgggagct ccatggcgtc gaggtggcag
     1681 tgggagtcga ccgtctcgac gctcaaaggc tgcggggcgg gcggcggcga gcctgcgatc
     1741 atgccggcgg ggcgtcgtcg gccaggcgcg ggaagagcga ctcgcccttg gtgatggcgg
     1801 cgccgaccgg cagctggccc cagcgaccgg cgtccgtcag gttctgcgtg gccagtggcc
     1861 cgagggcagg cgcggcacca agagcggtcc aaagcgcagc cgtcgctcgc gggatgaacg
     1921 ggttcagcac gacggccagc acccgcaatc cctcggcggc ggtgtagagg atcgtcgcca
     1981 cccgtgcccg cgaggcgggt gaggagtcga tctccttggc gaccttccac ggcgcctggt
     2041 cgctgaggta gccgttcagg gcaccgacga gctcccaaat cgcggccagg ccgccctgga
     2101 agtcgagtgc gagaaccgcc tcatcagcgg ccgccaccga acgcgacgca acgtcggcga
     2161 caacatcctc ggcgggcccg gcgtcctccg ccggcggcag cacgccgccg aagtacttct
     2221 cggtcatcgc cgtcacgcgc gacgccaggt tgccgaaccc gttcgccaac tcagatgtgt
     2281 agcgcgcact catgtcttcc cacgagaacg agccgtcctg tccgaactga atcgcgcgca
     2341 ggaagtaata gcgaaacgcg tcagacccga aatgatcggt gatctccgcc ggcgcaatgc
     2401 cggtgagctt cgtcttgctc atcttctcgc cgccgacgag cagccagccg tgcgcaaaga
     2461 ccttacgtgg cagcgcaacg ccggccgcga gaagcatcgc gggccagatc acggcatgaa
     2521 agcgcaggat gtctttgcca acaagatgca cgtcggcggg ccaggtctgc gcgaacttct
     2581 ccttgtcggc ggcgtctggc gcttcgagcc cgacggcggt ggcgtagttg agcagcgcgt
     2641 cgaaccagac gtagacaacc tgcgtgtcgt cccacggcac cttgatgccc cagtcgaatg
     2701 acgaccgcga gatcgaaagg tctgtcagac ccgaccggat gaacgacagc acctcgttgc
     2761 gtgcgctctc gggctcgacg gcttcggggc gcagttcgta gtgcgcgagc aacgcgtcgc
     2821 cgtacttcga gagccggaag aagtagttcg tctcgctgag ctcctcgacc ggccggccgt
     2881 ggatcgcgca caccttctga ccggcgtact cgccggtgcc gtcaagcagg tcgcccggca
     2941 gcttgaactc ctcgcagttc acgcagtacg gaccctcata aagaccctcg tagatctcac
     3001 cggcgtcata caggccctgc aagaacgcct gcacccgctc ggtgtgccgc agttccgtcg
     3061 tccggatgaa gtcgtcgttg gcgatgtcga gggagcgcca gaccggtcgc cactcctcct
     3121 cgaccagccg atcggcccac tgctggggcg agacatcgtg cgcttcggcg gttcggagca
     3181 ccttctcgcc gtgctcgtcg gtgccggtca aaaaccagac cgactctccg cgctggcggt
     3241 gccaacgggt cagcacgtcg ccggccaccg tcgtgtaggc gtgcccgatg tgcgggacgt
     3301 cgttgacgta gtagatcggc gtcgtcagat agaaggcctt ggcagcttcg gggcccgggt
     3361 tgtcggtcac accctcaagg ctaccgagcg cacgctgtgc atttct
//
LOCUS       DATCBJ010000603         2198 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_240957, whole genome shotgun sequence.
ACCESSION   DATCBJ010000603 DATCBJ010000000
VERSION     DATCBJ010000603.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2198)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2198)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2198
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_240957"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..333)
                     /locus_tag="VII50_12120"
     CDS             complement(<1..333)
                     /locus_tag="VII50_12120"
                     /inference="COORDINATES: protein motif:HMM:NF025199.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4191 family protein"
                     /protein_id="HEY5051638.1"
                     /translation="MPKPEKNVAPLKGRAARRARKAARAGRPKRSAQIREAFVITRRR
                     DKRLVPYLALAFAATLAFFELAGIVLGNPIVFSVFGVVSAVLVTFIVFGQRAQRSALG
                     EIEGQPGAA"
     gene            complement(342..1292)
                     /gene="lipA"
                     /locus_tag="VII50_12125"
     CDS             complement(342..1292)
                     /gene="lipA"
                     /locus_tag="VII50_12125"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HEY5051639.1"
                     /translation="MSAIAPEGRKLLRVEARNATVPIEKKPAWIKTRARMGPQYSALK
                     ELVRSEGLHTVCEEAGCPNIFECWEDREATFLIGGATCSRNCSFCQIDSGRPEPLDLD
                     EPRRVAESVATMGLRYATVTGVARDDLDDEGAWLYAQTVREIHATVPGCGVELLTPDF
                     HARPELLALVFDAQPEVFAHNLETVPRIFKSIRTGFRYERSLEVIDLARDRGLVTKSN
                     LILGLGEQRAEISSTLVDLRDAGCDLITITQYLRPSLRQHPVERWVEPAEFAELEAEA
                     RELGFAGVMSGPLVRSSYRAGRLYRDALAQRDAARIAPVS"
     gene            complement(1289..1954)
                     /gene="lipB"
                     /locus_tag="VII50_12130"
     CDS             complement(1289..1954)
                     /gene="lipB"
                     /locus_tag="VII50_12130"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: protein motif:HMM:NF010925.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HEY5051640.1"
                     /translation="MIAFRHLGVVPYEQAWEMQRDVHAARVAEQIPDTCLLLEHPPVF
                     TAGRRTQDFERPVDGTPVIDVDRGGRMTWHGPGQLVGYPIVRLPMPVDAVGHVRRLEE
                     ALIAVCADFGVIAIQVAGRSGVWVPGHGGAAARKIAAIGVRISRCTTMHGFALNCDPD
                     LSWFARIVPCGIDDADVTSLSRELGRDVSVDEARPLVERQLAAVLDATKASIATQPSR
                     VPA"
BASE COUNT          366 a          739 c          747 g          346 t
ORIGIN      
        1 cgccgcgccg ggctggcctt cgatctcgcc tagtgccgag cgctgcgcgc gctggccgaa
       61 cacgatgaac gtgaccagga cggcgctgac aaccccgaag acgctgaaca cgatcgggtt
      121 gccgagaacg attccggcaa gctcgaagaa ggcaagcgtc gcggcgaacg cgagcgcgag
      181 gtaggggact agccgcttgt cgcgccgccg ggtgatgacg aaggcttccc ggatctgcgc
      241 ggatcgtttc ggccggcctg ctctggcggc cttgcgagcc ctgcgggccg cgcggccttt
      301 gagcggcgcc acgttcttct cgggtttcgg catagtgcgg ctcaggatac gggcgcgatc
      361 cgcgcggcgt cgcgctgggc gagtgcgtcg cggtagagcc ggcccgcccg gtaggacgaa
      421 cgcacgagcg gtccggacat cacgccggcg aacccgagct cgcgggcctc ggcttcgagt
      481 tcggcgaact cggccggttc gacccagcgc tcgacggggt gctggcgaag tgacgggcgc
      541 aggtactgcg tgatggtgat caggtcgcag cccgcgtcgc gcaggtcgac cagcgtcgag
      601 ctgatctcgg cgcgctgttc acccaggccc aagatcagat tcgacttggt gaccagcccg
      661 cgatcgcgcg ccaagtcgat gacctcgagc gaccgctcat agcgaaaccc cgtgcggatc
      721 gacttgaaga tgcgcggcac ggtctcgagg ttgtgcgcga acacttcagg ttgcgcgtcg
      781 aagaccagcg cgagcaattc cggcctggcg tggaaatcgg gggtcagcag ctcgacgccg
      841 cagccgggca ccgtggcgtg gatctcgcga actgtctgcg catacagcca ggcaccctcg
      901 tcgtcgaggt cgtcgcgcgc gacacccgtg accgtggcgt agcgaagtcc catcgtggcg
      961 accgactccg caacccggcg tggctcgtca aggtccagcg gttcaggccg accgctgtcg
     1021 atctggcaga acgaacagtt acgcgagcag gtcgcgccgc caatcagaaa agtcgcttct
     1081 cggtcttccc agcattcaaa gatgttcggg cagccggcct cctcgcaaac tgtgtgcaac
     1141 ccctcgctgc gcacgagttc cttcaacgcg gagtactgcg gacccatccg ggcgcgggtc
     1201 ttgatccacg ccggcttctt ctcgatcggc accgtcgcgt tgcgtgcctc gacccgcagc
     1261 agcttgcgac cctcgggcgc gatagcgctc atgcgggtac cctgctgggc tgggtcgcga
     1321 tgctggcttt ggtcgcgtcg agcacggccg ccagctgccg ttcgaccaac ggacgtgctt
     1381 catcgacgct gacatcgcgg ccgagctcgc gcgaaagcga cgtcacgtcg gcgtcatcga
     1441 ttccacacgg cacgatccgg gcgaaccagc tcagatcagg gtcgcagttc aacgcgaagc
     1501 cgtgcatcgt cgtgcaacga gagatccgga cgccgattgc cgcgatcttg cgcgccgctg
     1561 ccccgccgtg accgggcacc cacactccgg accggccggc gacctggatc gcgatgaccc
     1621 cgaagtcggc gcataccgcg atcagggctt cctccagccg ccgcacgtgg ccgaccgcgt
     1681 cgaccggcat cggcaaccgc acgatcggat aaccgacaag ctgccccgga ccgtgccacg
     1741 tcatccggcc gccacggtcc acatcgatca ccggcgtgcc atcgacgggc cgctcgaagt
     1801 cctgggtacg acgacccgcg gtgaagaccg gcggatgctc caacagcagg caggtatcgg
     1861 ggatctgttc agccacccga gctgcgtgca cgtcgcgctg catctcccac gcctgctcat
     1921 aagggacgac gccgaggtgc ctgaacgcga tcacgttgtt caggatacgg ggttcgcacg
     1981 gcgcccgcgg gcgcgcaggg ctgtggacaa ccacagtggg cgggacgctt tgtgcggtgc
     2041 aatagcggcg tggacgactt atcgctgccc ggctacgacg tcgaagagct ggtggggttc
     2101 ggcggctcgg gcgaagtctg gcgggcgcgt gactcgtcga tcggtgaggt ggtcgcgctc
     2161 aaacggctgc gaagcgacgt cgcgaccgcg atctcaac
//
LOCUS       DATCBJ010000604         3357 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_78747, whole genome shotgun sequence.
ACCESSION   DATCBJ010000604 DATCBJ010000000
VERSION     DATCBJ010000604.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 3357)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3357)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3357
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_78747"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..625
                     /locus_tag="VII50_12135"
     CDS             <1..625
                     /locus_tag="VII50_12135"
                     /inference="COORDINATES: protein motif:HMM:NF015411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HEY5051641.1"
                     /translation="FRVSPGMRPDAESLCAGMRIALIGTGKIGGTLGQKFRQAGHEVV
                     YGSRHPGDDGPGGEPAAAVGEAIAGADVVVFAIPGPAVAAVVADHGAALAGKVVVDAT
                     NRVGQPVVNNRAEIVAAAPDALYVRAFNTLGWENFADPLPDADLFFAADPDARASAEA
                     LIEAVGLRPQYVGDADATATVDALLPFWFALVKQHGGNRKVALRVVD"
     gene            complement(644..1603)
                     /locus_tag="VII50_12140"
     CDS             complement(644..1603)
                     /locus_tag="VII50_12140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020671078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HEY5051642.1"
                     /translation="MADVLVLVDHVDGVVGKPTLELLTLAARIGSPVAVVVGGSGGMT
                     EKLGEYGAAAVLTVDDDALTSAVVAPIVSLLESLVASRSPAAVLIASTTEGKEIAGRL
                     AVRLDAGVLTDAVDVSLEGGAVVVEQSVFGGSTVVHAQATRGVPIVTVRPNSVTPVAA
                     PVTPDVSAVTAPAIDGRHGVVESRSAAASGGRPGLSEATVVVAGGRGVGTAEKFALIE
                     ELADALSAAVGASRAATDAGWYPHAAQVGQTGVTVAPQLYIACGISGAIQHRAGMQTS
                     KTIIAINKDAEAPIFELADFGVVGDLHVVVPQLIAEIARRKGS"
     gene            complement(1618..2427)
                     /locus_tag="VII50_12145"
     CDS             complement(1618..2427)
                     /locus_tag="VII50_12145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HEY5051643.1"
                     /translation="MSSGAMRIVVCVKQVPDTWSEKRLDPADQTLDRAAADGVIDELD
                     TYAVEEALRIKEANEGSAVVVLTMGPAGAADTVRKALSMGADEAVHVVDDALHGSDAL
                     ATSLVLAKAIERTGFDLVVLGSESTDARMGVVPAMLAERLGVPQATLASKVEVDASSD
                     PATVRVHRQTDVGQALVSAQLPAVISVLEKINEPRYPSFKGIMAAKTKPTATWSLADL
                     GIDASSVGLVAAATVVVDATQRPPRGERTIIKDDGEAGSRIADFLVSRKLL"
     gene            2479..>3357
                     /locus_tag="VII50_12150"
     CDS             2479..>3357
                     /locus_tag="VII50_12150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF885 domain-containing protein"
                     /protein_id="HEY5051644.1"
                     /translation="MDQRLRSVCDLFVGDLREGSGLHEYDGLIQDLSPGGVRAGLAKL
                     GGPPLDDPHDEAHLRAFEDAARLAFGQLEVHRSNPATHLPNFDLSCYDRDYAPASDRA
                     AAKRAHIAQWPDLVDMTIEALDAVPAPLATALLGAFTGLVAVINPTEQGASAALTSHA
                     RLMAHLHDAAEHGAPDASLGAAGLASLMGSVEAMTVDIGRLAEIADSERDRLMTMLRE
                     ACTRIDRNATLAQAVAALVADHPDADGVLTEAQAQVDEVLGFTREHDLVPYTDGECIV
                     TTSPESRRWAMAMMAWA"
BASE COUNT          579 a         1161 c         1088 g          529 t
ORIGIN      
        1 gtttcgggtc agcccgggaa tgcgcccgga cgccgagtcg ttgtgcgccg gtatgcggat
       61 agcactcatt ggaacaggca agatcggtgg cacgctcggc cagaagttcc ggcaggcagg
      121 ccacgaggtt gtgtacgggt cgaggcaccc gggcgacgac ggccccggtg gcgagccggc
      181 cgcggccgtc ggcgaagcga tcgccggcgc ggacgtggtg gtgttcgcga tcccgggccc
      241 agcggtagca gcggtcgtgg cagaccacgg cgcagcgctc gcgggcaagg tcgtggtcga
      301 cgcgacgaac cgggtgggcc agcccgtcgt caacaaccga gccgagatcg tggccgcagc
      361 tcctgacgcg ctgtacgtgc gggcgttcaa cacgctgggc tgggagaact tcgctgatcc
      421 actccccgac gccgacctct tctttgcggc cgacccagac gcccgagcaa gcgccgaggc
      481 gctcatcgag gccgtcggat tgcgacccca gtacgtcggc gacgccgacg caacggcgac
      541 agtcgacgcc ttgttgccgt tctggttcgc gctggtgaag cagcacggcg gcaaccgcaa
      601 ggtggcactg cgcgtcgtcg actagcggcc cgcgccgagc ttgctaggaa cccttgcgcc
      661 tggcgatctc ggcgatcagc tgtggcacga cgacgtgcag atcaccgacg acaccgaagt
      721 cggcgagttc gaagatcggc gcctcggcat ccttgttgat cgcgatgatc gtcttcgatg
      781 tctgcatgcc ggctcggtgc tggatcgcac ccgagatccc gcacgcgatg tagagctgcg
      841 gcgcgaccgt gacaccggtc tgccccacct gcgcagcgtg cgggtaccag cccgcatcag
      901 tcgctgcgcg tgaggcgccg accgccgccg acaacgcgtc ggcaagctcc tcgatgaggg
      961 cgaacttctc cgccgtcccg accccacggc cgccagcgac cacaaccgtc gcctctgaga
     1021 ggccaggccg accacctgat gcggcggccg aacgcgactc gacgacgccg tgccgtccgt
     1081 cgatagcagg agccgtcaca gccgaaacgt caggagtcac cggagccgca acaggagtga
     1141 cgctgttcgg ccggaccgtc acgatcggca caccacgcgt cgcttgcgcg tgcacgacgg
     1201 tcgatccacc gaagacggac tgctcgacca ccaccgcccc accctccagc gagacgtcga
     1261 cggcatcggt cagaacgcca gcatcaagac ggacggcgag gcggccggcg atctccttgc
     1321 cttctgtggt cgacgcgatc agtaccgctg caggcgaccg cgatgcgaca agcgactcga
     1381 gcaaagacac gatcggcgcg acgacagccg aggtcagcgc atcgtcgtcc acggtgagta
     1441 ccgcagccgc gccatattca ccgagcttct ccgtcatgcc gccactgccc ccgacaacca
     1501 cggcgaccgg cgagccgatg cgcgccgcaa gcgtaagcag ttcgagtgtc ggtttgccga
     1561 cgacaccatc gacgtggtcg accagtacaa gcacgtccgc catgacgaat cccgtcgtta
     1621 gagaagcttg cgcgagacga ggaagtcggc gatgcgcgaa ccggcctcgc cgtcgtcctt
     1681 gatgatggtg cgttcgccac gcggcgggcg ctgcgtcgca tcgacgacga cggtagccgc
     1741 cgcgaccaaa ccgacggacg aggcgtcgat gccgagatct gcaagcgacc aggtcgctgt
     1801 cggcttcgtc ttcgcagcca taatcccctt gaacgatggg tatctcggct cattgatctt
     1861 ttcgaggacg ctgataacag ccggcagttg cgccgagaca agggcctgcc cgacgtccgt
     1921 ctgccgatgc acccgcaccg tcgccgggtc gctcgatgcg tcgacctcga ccttgctggc
     1981 cagcgttgcc tgtgggaccc cgaggcgctc ggcaagcatt gctggcacga cgcccatgcg
     2041 tgcatccgtc gattcagacc cgaggacgac caggtcgaaa ccggtgcgct cgatggcctt
     2101 cgccaggacg agtgaggtag ccagcgcgtc ggatccgtgc agggcatcgt cgacaacgtg
     2161 cacggcttcg tccgctccca tcgagagcgc ctttcgcacc gtgtccgcgg cccccgccgg
     2221 ccccatcgtc aggacaacga cggcactccc ttcgttcgct tccttgatgc gcaacgcttc
     2281 ttcgaccgcg taggtatcga gttcgtcgat gacgccgtcc gcggccgcac ggtcaagcgt
     2341 ctgatcagcg gggtcgagcc gcttctccga ccaggtgtcg ggtacctgct tgacgcagac
     2401 cacaatcctc atcgcaccac tactcatggg tcgcgcctcc tgtcggccga ctctaccggg
     2461 cggcgctaac gtgggctcat ggaccagcga ctgcgctcgg tgtgcgacct gttcgtcggt
     2521 gacctccgcg aaggttctgg cttgcacgag tacgacggcc tgattcaaga cctgtcaccc
     2581 ggcggcgtcc gcgctggcct cgcgaagctc ggcggaccgc cgctcgacga cccgcacgac
     2641 gaagcccacc tgcgggcgtt cgaagacgcc gcccggctcg cgttcggaca actggaagta
     2701 catcggtcca acccggcgac gcacctaccg aatttcgacc tctcctgtta cgaccgtgac
     2761 tacgcgccgg caagcgaccg cgcggctgcc aaacgcgcac acatcgcgca atggcccgac
     2821 ctggtcgata tgacgatcga ggcactcgac gcggtcccgg cgcctctcgc aacggcgctc
     2881 cttggcgcgt tcaccggact cgtcgctgtg atcaacccga ccgagcaggg ggcgtcggcc
     2941 gcgttgactt cgcacgccag actgatggct catctccatg atgcagcgga gcacggcgca
     3001 cccgacgcgt cgttgggcgc agccggcttg gcttcgttga tgggttccgt cgaagcaatg
     3061 accgtcgaca ttggccggct cgctgaaatc gcagatagtg aacgtgatcg gctgatgacg
     3121 atgctgcgcg aggcatgtac gcggatcgat cgaaacgcca ctctcgcgca ggcggtagcc
     3181 gccctcgtcg ctgaccatcc ggacgccgat ggcgtgctca ccgaggcgca ggcgcaggtc
     3241 gacgaggtcc tcgggttcac ccgcgaacac gacctcgtgc cgtacacaga cggcgagtgc
     3301 atcgtgacga cctcgcctga gtcgcgacgg tgggccatgg cgatgatggc gtgggct
//
LOCUS       DATCBJ010000605         3539 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_186259, whole genome shotgun sequence.
ACCESSION   DATCBJ010000605 DATCBJ010000000
VERSION     DATCBJ010000605.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 3539)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3539)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3539
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_186259"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(171..2339)
                     /locus_tag="VII50_12155"
     CDS             complement(171..2339)
                     /locus_tag="VII50_12155"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: protein motif:HMM:NF003922.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA degradosome polyphosphate kinase"
                     /protein_id="HEY5051645.1"
                     /translation="MTQLSQSGSPVDASDYGEDLVEALGSAVTEHHVDADPDLPTDRF
                     IDREASWLDFNMRVLELAQRDDVPLLERVKFLAIFATGLDEFFMVRVAGLKRRIATGI
                     AVTAPSGLSARDQLELVSTRAHALATHHARAFFDEIKPALAERGIQLLRWEDLDEADH
                     TQLRTFFSESVYPVLTPLAVDPAHPFPYISGLSLNLAVVVRDPRTGEERFARVKVPEI
                     LPRFLPLGGGRFVTIEEVIAAHLQVMFHGMEVLESHRFRVTRNQDLEVDEDDVDNLLQ
                     ALERELLRRRFGPPVRLEVEETTSPRVLDLLIRELGVNETEVYRLPAPLDLAGLWSIS
                     ELQRPELKYEQFVPATHPRLVNADGDGVRDIFAALRSGDILVHHPYDSFATSVQAFVE
                     QAATDADVLAIKQTLYRTSGDSPIVDALIDAARAGKQVLVLVEIKARFDEEANIRWAR
                     KLEQAGCHVVYGLVGLKTHCKLCLVVRREPDGSLRRYAHLGTGNYNPKTARMYEDVGL
                     LTADPAIGGDVADLFNHLSGYTRPDPYKTLLVAPDTVRTGLTAMIRREAGNATAGRPS
                     GITMKVNSLVDEELIDELYAASRAGVSIDLIVRGMCALRPGVPGLSENVRVRSILGRF
                     LEHARIMRFRGGGDDEYWIGSADLMHRNLDRRVETLVRVVDPEGRRILDNVLDRSMSP
                     EIAAWELGPDGTFAATKGVDLQGEFVRSHRAQSHDGPVVS"
     gene            complement(2366..2905)
                     /locus_tag="VII50_12160"
     CDS             complement(2366..2905)
                     /locus_tag="VII50_12160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5051646.1"
                     /translation="MTFTRSTGSRYGVDVMRESGPPARLRVVKMDGDWLPREVAHFLV
                     EREFGLKLGIFGQLAAGGDAGRFWAAPADRDSRLAHLGHRLQVTGRGDLRRSVALVAW
                     CTAAWEVETGRRSAGQPGTQSKGPWPLLDVAAVPHDQLAGAVRSLAAAAVAWNDLQPG
                     QRVSYEWPLALTLTARRSA"
     gene            complement(2984..>3539)
                     /locus_tag="VII50_12165"
     CDS             complement(2984..>3539)
                     /locus_tag="VII50_12165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5051647.1"
                     /translation="ALSISSCVVLAVVATFPVVDRVTLESRLSNAASTLLGMHVEVDC
                     QTLTGSALDLGRELGYVKWGPGGIPEHKTLIKHQQCNDLHSYLDSSKQRPSAGEILAV
                     HVLTHESMHMSGITSEFIAECAAMQHDTAMAEDLGASPVAAHALAVTYWLTVYPDMPD
                     GYRSADCRPGGSLDLHLPSAPWAA"
BASE COUNT          656 a         1181 c         1115 g          587 t
ORIGIN      
        1 ccgcgttcat cgagatgtgc gtcggcgcat cgatactggc cacttcgggc accacgccgg
       61 cgatcaacgg cagttcaaac gtcgggttca cccggaactt ggcctcggtc tcccgcacag
      121 tccgggggct cacgtccctt gccacgaggc gagtctaccc agcagccgca tcagctaacg
      181 accggtccat cgtggctttg cgcgcgatga ctgcgcacga actcaccttg caggtcgacg
      241 cccttcgtcg ccgcgaaggt tccatccggc cccaactccc acgctgcgat ctccggagac
      301 atcgagcggt cgagaacgtt gtcgaggatg cggcggccct ctgggtcaac aacccggacc
      361 agcgtctcga cccggcggtc gagattgcgg tgcatgaggt cggcactccc gatccagtac
      421 tcatcgtcac cgccgccgcg aaagcgcatg atgcgcgcat gctcgaggaa cctgccaaga
      481 atgcttcgca cccgcacgtt ctccgacagg cccggcacgc ctgggcgcag cgcgcacatc
      541 ccacgcacga tcaagtcgat cgagacaccc gctcgtgacg ccgcgtacag ctcgtcgatc
      601 agttcctcgt caaccaggct gttcaccttc atcgtgatgc cacttggccg cccggcagtt
      661 gcgttgccgg cctcgcgacg aatcatcgcc gtgagtcccg tgcgtacggt gtcgggcgca
      721 acgagcagtg tcttgtacgg atcgggcctg gtgtagcccg ataggtgatt gaacaagtca
      781 gcgacgtcac cgccgattgc cgggtcagcc gtgagcaagc cgacgtcctc gtacatacga
      841 gcggtcttcg ggttgtagtt gccggtgccc agatgcgcat accgacgcag cgatccgtcc
      901 ggctcacgac gtacgacgag acacagtttg caatgcgtct tgagtccgac gagaccgtag
      961 accacatggc aaccggcctg ctcgagtttg cgcgcccagc gaatatttgc ctcttcgtca
     1021 aagcgtgcct tgatctcgac cagcaccaac acttgcttgc ccgcgcgcgc cgcatcgatg
     1081 agtgcgtcga cgatcggtga gtctccactc gtgcggtaca acgtctgctt gatcgccagc
     1141 acatcggcat cggtagcggc ctgctcgaca aatgcctgca cactcgtcgc gaacgagtcg
     1201 tacgggtggt gcacgagaat gtcaccagac cgtagagcgg caaaaatatc gcggacgccg
     1261 tcaccatcgg cgttgaccaa cctcgggtgc gtggccggca cgaactgctc gtacttgagc
     1321 tccggccgtt gcagctcgga gatgctccac aagccggcaa ggtcaagcgg ggcaggaagt
     1381 cggtacacct cggtctcgtt cacgccgagt tcgcgaatca gcaaatcaag cactcgcggg
     1441 ctggtcgtct cctcaacttc gaggcgcacc ggcggcccga atctgcgacg caaaagttcg
     1501 cgttcgagag cctgcaacag gttgtcgaca tcgtcttcgt cgacttcgag atcctggttg
     1561 cgggtcaccc ggaaacggtg gctctcgagc acctccatgc cgtgaaacat cacctgcaga
     1621 tgcgcggcga tgacctcttc gatagtgacg aaccgaccgc cgccaagtgg cagaaagcgc
     1681 ggcaggatct cggggacctt gacgcgcgcg aatcgctcct caccggtgcg cggatcgcgg
     1741 acgacgacag caaggttcag cgacagtccg gagatgtagg ggaacgggtg cgctggatcg
     1801 acggccagtg gcgtgagcac cggatacacg ctctcgctga agaaggtccg caattgcgtg
     1861 tggtcagcct cgtcgagatc ctcccaacgc agcagctgga tgccgcgctc ggcgagcgcc
     1921 ggcttgatct catcgaagaa agctcgggcg tgatgagtcg ccagtgcgtg cgcgcgcgtt
     1981 gacaccagct cgagttggtc acgcgccgac aagccactgg gggccgtgac agcaatgccg
     2041 gtcgcgatcc gccgcttcaa gccggcaacc cgcaccatga agaactcgtc gaggccggtc
     2101 gcgaagatgg cgaggaactt cacccgctcg agcaacggca catcgtcacg ctgcgccaac
     2161 tcgagcactc gcatgttgaa gtcgagccag ctcgcctcgc ggtcgatgaa gcggtcggtc
     2221 ggcaggtccg ggtcggcatc cacatgatgt tcggtgaccg cgctgccgag tgcttcgacc
     2281 aagtcctcgc cgtagtcgga cgcatcgacc ggcgagccgg attgcgaaag ctgcgtcacg
     2341 tcgacctcct gctccgtacg ggagtctagg cggagcgtcg tgcggtaagc gtcagggcca
     2401 gcggccactc gtacgagacc cgttgcccgg gttgcaggtc gttccaggcg actgccgccg
     2461 cagcgagcga gcggacggcg ccagcgagct ggtcatgcgg cacggccgcc acatccagca
     2521 gcggccacgg ccccttggac tgagtccctg gctggcccgc tgaccggcgt ccggtctcga
     2581 cctcccaggc cgcggtgcac cacgcgacca gtgcgaccga ccggcgcaag tcgccacgtc
     2641 cggtgacctg aaggcgatgc ccaaggtgag ccagtcgcga gtcgcggtcg gcaggtgcgg
     2701 cccagaaccg gccggcgtct cctccggcag caagctggcc aaagatgccg agcttgaggc
     2761 cgaactcgcg ctccaccaga aagtgcgcga cctcacgcgg gagccagtcg ccgtccatct
     2821 tgaccacccg caaccgcgca ggcggcccac tctcacgcat cacatcgacg ccgtaccggg
     2881 acccggtcga ccgggtgaaa gtcacgcgca cgccttgagg ctacggaccg ctacgcccgc
     2941 tgtcccctgg aataccgcta tgccgcggag cactacatca gggctaggcc gcccacggcg
     3001 cgctgggcag gtgcagatcc aacgacccac caggccggca gtccgcgctc cggtacccgt
     3061 cgggcatgtc cggatagacc gtcagccagt acgtgacggc taaggcgtga gcagccaccg
     3121 gggaggcgcc cagatcttcg gccattgcgg tgtcgtgctg catggcggcg cattcggcga
     3181 tgaattcgga ggtgatcccg gacatatgca tcgactcgtg ggtcagcacg tggacagcca
     3241 ggatctcccc ggcactggga cgttgcttgc tcgaatcgag atagctgtgc agatcgttgc
     3301 actgctgatg cttgatcagc gtcttgtgct cggggatccc gcccgggccc catttcacgt
     3361 agcccagctc ccggccaagg tcgagcgcgg atccggtgag cgtctgacag tcgacctcga
     3421 catgcatacc gagcaaggtg gacgccgcgt tgctgaggcg gctctcgagg gtgaccctgt
     3481 ccaccacggg aaaggtcgcg acgaccgcaa gaaccacgca ggaactgatg ctgagcgcg
//
LOCUS       DATCBJ010000606         3098 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_538748, whole genome shotgun sequence.
ACCESSION   DATCBJ010000606 DATCBJ010000000
VERSION     DATCBJ010000606.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 3098)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3098)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3098
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_538748"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..213)
                     /gene="fdxA"
                     /locus_tag="VII50_12170"
     CDS             complement(<1..213)
                     /gene="fdxA"
                     /locus_tag="VII50_12170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003083029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HEY5051648.1"
                     /translation="MTYVIAQPCVDLKDRACVDECPVDCIYEGGRMLYIHPDECVDCG
                     ACEPVCPVEAIFYEDDVPDKWKEYYKA"
     gene            295..1266
                     /locus_tag="VII50_12175"
     CDS             295..1266
                     /locus_tag="VII50_12175"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY5051649.1"
                     /translation="MSINLRPDDVGARVSVRHRLPSGPISGLLSGATDVVGDLEILDA
                     DQLAVRQSDGSLVAIEAALVVAARVVGPSLLSARELEAVSGRSWPAPDEEWLGRWWLR
                     SAGGFTARAGAVRPLGDPGRPLDDALAYVISWYAERGLPAMIRAVSGSNVSLELDRRG
                     WTVANESLVQTVTLARLARLLAARDAIARPPAAVQTTTLPPNPWLLRYHGGSITPVAL
                     EVLTGAQDVSFATIEAADPTAAATAIGRAAVEPPWVGFAAIEVDPSVRRQGHGRAVMS
                     GLTQWAASRGAVRACLEVLADNHAALALYESLGFAEHHRYTYRSPPA"
     gene            complement(1279..1743)
                     /locus_tag="VII50_12180"
     CDS             complement(1279..1743)
                     /locus_tag="VII50_12180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5051650.1"
                     /translation="MPPGGRTLDTNDPDSVWMAQNVIDVFAQEVEAVAQDSPDYCDVA
                     VDGFHDGMTVWWHGTPSSAVLGVLVRARHAGITPVVLPTPIERRTLDAAVDRLSNRMQ
                     ELGMTELARSSGCAGIDVGLIVLTPQSEADIAAAAGPGIPLHFSETGPASPA"
     gene            1724..2008
                     /locus_tag="VII50_12185"
     CDS             1724..2008
                     /locus_tag="VII50_12185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5051651.1"
                     /translation="MRPPGGIGGTVPSEPGLGVGIGCGVPDPTSTGEDAATGGDTATG
                     GDTTTVDGPTAVPVGAVPVGAVPTLAHAVSTSALAAAASHFIEPPADYDS"
     gene            2058..2654
                     /locus_tag="VII50_12190"
     CDS             2058..2654
                     /locus_tag="VII50_12190"
                     /inference="COORDINATES: protein motif:HMM:NF024986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HEY5051652.1"
                     /translation="MTTAPVDRPAGDLLAFGEAELVGVRFVGGAYAGADLIELRTRGV
                     VFEDCDFSHARLSSSEHVGSAFLRCTFRDAVLRDSVFDGCKLSGSDLSGAALRPMRVV
                     GGDWSYVTIRGTDLTDLDFTGVKLAEADLSESALHRAVLRDCDLRHARVHHTEFGDAD
                     LRGAELDGVDLLAASWRGTHVDLAQSGLFAIALGLVVD"
     gene            2692..>3098
                     /locus_tag="VII50_12195"
     CDS             2692..>3098
                     /locus_tag="VII50_12195"
                     /inference="COORDINATES: protein motif:HMM:NF015862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-degrading domain-containing protein"
                     /protein_id="HEY5051653.1"
                     /translation="MADDPTGITLEQVAAEEAELRFRRFDFDDAWRLGTMLVETGRAQ
                     RSPIAIDISRGGQQLFHAGLPGSTPDNDAWIQRKVASVLRFGRSSMALSLERKLDGRT
                     LAEASFVDPQKFAAAGGCFPVHIEGIGIVGTVAV"
BASE COUNT          471 a         1042 c         1042 g          543 t
ORIGIN      
        1 cgccttgtag tactccttcc acttatccgg gacgtcgtcc tcgtagaaga tcgcctcgac
       61 cgggcacacc ggctcgcagg cgccgcagtc gacgcactcg tcggggtgga tgtagagcat
      121 ccggcccccc tcgtagatgc aatcgaccgg acactcgtcg acgcacgccc tgtccttgag
      181 gtcgacacag ggctgggcga tcacgtacgt cacggggagt cccttcctcg aaagcggtga
      241 accgcaccta gtatcggcga acctgaccgc ctgtcgatgc ttggggtgtc cggcgtgtct
      301 atcaatctgc gccccgacga cgtcggtgcc cgggtcagcg tgcgtcaccg cctgccttcg
      361 ggcccgattt cgggcctgct ttcgggcgca acggacgttg tgggcgatct ggagattctg
      421 gacgccgatc agctggcggt tcggcaatct gacggctcct tggtggcgat cgaggcggcc
      481 ttggtcgttg ccgcgcgagt ggtcgggccg agcctcctgt cggcccgcga actcgaagcg
      541 gtgagcggcc ggagctggcc tgcgcccgac gaggagtggc tcggccggtg gtggttgcgg
      601 tccgcgggcg gcttcacggc tcgtgctggc gcggtgcgtc cgctaggcga tcccggccgc
      661 ccgctcgatg acgcgctcgc ctacgtcatc agttggtacg cagaacgagg cctacccgcc
      721 atgatccggg cggtgagcgg cagcaacgtg agtctggagc tcgaccgtcg tggttggacc
      781 gtggctaacg agtccttggt tcagacagtg accctggctc ggttggcgcg tttgctcgcg
      841 gcccgcgacg ccattgcacg gccgcctgct gcggttcaga ccacgacgct cccgccgaat
      901 ccatggctgc tgcgatacca cggcggttcg atcactccgg tcgcgcttga agtactgacc
      961 ggcgcacagg acgtctcatt cgcgacgatc gaggctgctg acccgaccgc cgctgccacc
     1021 gccattgggc gagcagcagt agaaccgccg tgggtgggct tcgcagccat cgaggtcgac
     1081 ccgtccgtgc gccgacaagg ccacggtcgt gcggtcatgt cggggttgac gcagtgggcg
     1141 gcttctcgtg gcgctgtccg tgcttgcctc gaagttctcg ccgacaacca cgccgcgttg
     1201 gcactgtacg aatcgctcgg cttcgccgag caccaccgct acacctaccg gtccccgccg
     1261 gcataacgcg cgacgctgct acgccgggga cgctgggcca gtttcgctga agtgcaaggg
     1321 tattcccgga ccagccgccg cagcgatatc cgcttccgac tgtggcgtca agacaataag
     1381 cccgacgtct atgccagcgc acccgctgct acgtgccaac tccgtcatcc cgagctcctg
     1441 catgcggttc gacagtcggt cgaccgccgc gtccagggtt cggcgctcga ttggggtcgg
     1501 caacaccacc ggcgtgatgc ctgcgtgccg cgcacggacc aagaccccga gcacggccga
     1561 cgagggcgtg ccatgccacc acaccgtcat gccatcgtgg aacccgtcga cagcgacatc
     1621 gcagtagtcg ggcgagtctt gcgccaccgc ctcgacttcc tgggcgaaca cgtcaatcac
     1681 gttctgcgcc atccacacgc tgtccggatc gttggtgtca agcgtgcggc cgcccggcgg
     1741 catcggcggc actgttccct cggagccagg actcggcgtg ggcatcggct gcggggttcc
     1801 cgatccaaca tcgaccggcg aggacgctgc gaccggcggg gacactgcga ccggcgggga
     1861 caccacgacg gtcgacggcc caacggccgt cccggttggc gcggttcccg ttggtgccgt
     1921 cccgacactg gcgcacgcgg tcagcaccag cgcgctagcc gcagccgcga gccacttcat
     1981 cgaacctccc gccgactatg actcatgaag cgcgatggcc ggttccagta acgtcgcacg
     2041 ggttggtgag gagagcaatg acaacggcac ccgtggaccg tccggccggt gacctgctgg
     2101 cattcggcga ggcggagctg gtcggcgtcc ggttcgtcgg cggtgcctat gcgggagccg
     2161 atctgattga gctccggacc cgtggcgtgg tgtttgagga ttgcgacttc tcccacgccc
     2221 gcctatcaag cagcgagcac gttggttcgg cgtttctgcg ttgcacgttt cgtgacgcgg
     2281 tgctgcgcga ctccgtcttc gacggatgca agctgagcgg aagtgacctt tccggcgcgg
     2341 cgctccggcc gatgcgcgtc gtcggaggcg actggtcgta cgtcacgatc cgcggaaccg
     2401 acctgaccga cctggacttc accggcgtaa aacttgccga agcggacctt tccgaatcgg
     2461 cgctgcatcg cgcggtgctg cgcgactgcg accttcggca cgcgcgggtg catcacaccg
     2521 agttcgggga cgccgacctg cgcggtgccg agctcgacgg cgtcgacctg ctcgccgcgt
     2581 cctggcgagg cacgcacgtc gacctcgcgc agagtgggct gttcgcgatc gcactcggtc
     2641 tcgtggtgga ctaggcaccc acaagcaact ttcgcgagac gggtcgaact gatggctgac
     2701 gatccaaccg gcatcacgct ggagcaggtg gcggcagaag aagccgaact gcgcttccgt
     2761 cgcttcgact tcgacgacgc gtggcggctc ggcacgatgc tcgtcgagac gggccgcgcg
     2821 cagcggtcgc cgatcgcgat cgatatcagc aggggtggcc agcagctgtt ccacgcgggt
     2881 ctgcccggat cgacgcctga caacgacgcg tggattcaac ggaaggtcgc gtcggtgttg
     2941 cgcttcggac gcagctcgat ggcgctgagc cttgagcgca agctcgacgg tcgcacgcta
     3001 gcggaggcat cgttcgtcga cccccagaag tttgccgccg ccggcggctg tttccctgtg
     3061 cacatcgaag gcatcggcat cgtcgggacg gtcgccgt
//
LOCUS       DATCBJ010000607         2362 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_238512, whole genome shotgun sequence.
ACCESSION   DATCBJ010000607 DATCBJ010000000
VERSION     DATCBJ010000607.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2362)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2362)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2362
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_238512"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(136..1155)
                     /locus_tag="VII50_12200"
     CDS             complement(136..1155)
                     /locus_tag="VII50_12200"
                     /inference="COORDINATES: protein motif:HMM:NF013862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HEY5051654.1"
                     /translation="MSQSHESLPSTGSKAQSFTLDSVRRTPQPQVVRNVALVLGGGGA
                     AGNAWEIGVIAGLAEAGLDMTEAADLVVGTSAGATAAAQVRSGKPAAELLVSVLSPPV
                     QPVGQNRERPPSLPIATVFERMRAIGAAATSAADLRRAMGAFGLESDSTLEPGAGQRR
                     AMVAARLPRPEWPDRPMIVVAVDAHTGELAAFDRDSGVDLVDAVTASCALPGLVPTHS
                     INGTCYIDGGVRSKENADLASGYAHVVVLSPLDGRIRTPTERAAHPAGQFEGLRRPPE
                     WGVDLASEVETLRKQGSHVEVIAPDADSRAAMGTNQMDPATRIPAARAGFAQGQQEAT
                     RVTFL"
     gene            complement(1273..1593)
                     /locus_tag="VII50_12205"
     CDS             complement(1273..1593)
                     /locus_tag="VII50_12205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5051655.1"
                     /translation="MRDAVRTSNARLSPMVGARPGWVGCGGVASGVVAEGRAWGWSRS
                     NGRLEELDSRDDELWLLGLNEARRVDNFVLSVAPSFAAELHARLFFKLSSFRSAVAAH
                     GLRR"
     gene            1736..2092
                     /locus_tag="VII50_12210"
     CDS             1736..2092
                     /locus_tag="VII50_12210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009075328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEY5051656.1"
                     /translation="MQLTEERLGATFTALADPTRRAIIARLARGDATVNELAAPFEMT
                     LPGISKHLKVLERCGMISRSRHAQFRPCHLERAVLDGAVEWIEEARRVWSERFDKLDV
                     HLGEIRATHRDKEGSP"
     gene            2089..>2362
                     /locus_tag="VII50_12215"
     CDS             2089..>2362
                     /locus_tag="VII50_12215"
                     /inference="COORDINATES: protein motif:HMM:NF019928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HEY5051657.1"
                     /translation="MSNQEATPGELEFIRVFDAPRELVFRCMIEPEHLTHFWGPTGVS
                     TPLEHIHVDARPGGAFETVMVNDDDSSEYVSRAVYVEVSEPERLVWR"
BASE COUNT          426 a          780 c          768 g          388 t
ORIGIN      
        1 tgtccggtcg cgatcgtcgt tggaatgtct gggccgaatc acggatccgg gccgcgccgg
       61 tgggaacggt ggcagaggac ggttaccggg ctcttcacag ctgacggtgt agtgctgggc
      121 gccggcgtgc aatcgtcaga gaaatgtcac gcgggtcgct tcctgctggc cttgggcgaa
      181 accggcccgg gcggcaggga tgcgggtcgc cggatccatc tggttggtgc ccatcgcggc
      241 tcgggaatcg gcgtccggtg cgatcacctc gacgtggctg ccctgcttgc gtagggtctc
      301 gacctcgctc gccaggtcca cgccccattc cgggggtctg cgaaggccct cgaactggcc
      361 tgccgggtgc gcggcccgtt ccgttggtgt ccggatccgt ccgtccaacg gagagagcac
      421 cacgacgtgc gcatagccag aggcaaggtc ggcgttttct ttggagcgca cgccaccgtc
      481 gatgtagcaa gtgccgttga tgctgtgtgt gggcaccaag ccgggcagcg cgcagctcgc
      541 ggtgaccgcg tccaccaggt cgacgccgga atcgcggtcg aacgcagcca actcgcccgt
      601 atgcgcgtcg acggccacca cgatcatcgg cctgtccggc cattcagggc gaggcaaccg
      661 ggcggcgacc attgcgcgcc gctgtcccgc cccgggttcg agtgtggagt cgctctccaa
      721 cccgaacgcg cccatcgcac gtcgtagatc ggctgccgag gtggcggcgg cgccgatggc
      781 cctcatccgc tcgaacactg tggcaatcgg cagggacggc ggccgttccc ggttctgtcc
      841 gaccggttga accggcgggg acagtaccga gaccaacagt tcggccgccg gtttcccgct
      901 gcgcacctgc gctgccgcgg tggcaccggc cgaggtgccg acgaccaaat cggcggcctc
      961 tgtcatatcg agaccagctt cggccaggcc tgcgatgaca ccgatctccc acgcatttcc
     1021 ggcagcccca ccgccaccga gaaccagagc aacgttgcga accacctgcg gctgcggcgt
     1081 acgtcgcacc gagtcaagcg tgaaggattg ggctttgctc ccggtcgacg ggagcgattc
     1141 gtgagattgc gacatgtctt ggtccctttc agggcatccg agatgcgggc acccaaggac
     1201 cgatgacgac gaagcgctag aggctccagc atgcagaggt gagcacccga ctgtgaacga
     1261 aaagaatcgg actcacctcc ttagaccgtg cgctgcgaca gcgctcctga acgaactgag
     1321 cttgaaaaat agtcgggcat ggagctccgc cgcaaacgac ggagcaacgg acagtacgaa
     1381 gttgtcgacc cggcgcgctt cgttcaggcc gagcaaccac aactcgtcgt cacgcgaatc
     1441 caactcctcc aggcgaccat ttgaccgcga ccatccccag gcccgccctt cggcgaccac
     1501 accggacgcg acgccgccgc agccaaccca acccggccgg gcgcccacca tcggcgaaag
     1561 ccgtgcattc gacgtccgaa ctgcgtcccg catggcgaac cgcttgcagt cgtcattcag
     1621 gacccggtgg gtgcccgaac tacctgacgg cgatcgctac cgagggttga ccgcgtggta
     1681 gtttacttaa cagataacta accaagtggt aaagtacggg ggtgaggtac acccaatgca
     1741 actgaccgag gagcgactcg gcgccacatt caccgcctta gcggatccga cccgccgagc
     1801 gattattgcc cgcctggcgc ggggggacgc cacggtgaac gagctggccg caccattcga
     1861 gatgacgctg ccagggatct ccaaacatct gaaggtgctc gagcgctgcg ggatgatctc
     1921 ccgttcgcgt cacgcgcagt tcaggccctg ccacctggag cgcgcggtgc tcgacggggc
     1981 tgtcgagtgg atcgaggagg ctcgacgggt ctggtccgag cgcttcgaca aactcgacgt
     2041 gcatctcggc gagatccggg ccacgcacag agacaaggag ggctcgccgt gagcaaccag
     2101 gaggcgacgc ctggcgagct ggagttcatc cgtgtcttcg acgcgccgcg cgagctcgtc
     2161 ttccgctgca tgatcgaacc cgagcacctc acccacttct ggggcccgac cggtgtgagc
     2221 actcccttgg aacatatcca cgtcgatgcc cgtcccggcg gagccttcga gacggtcatg
     2281 gtgaacgacg acgactccag cgaatacgtc tcgcgggccg tttacgtcga ggtgtcagag
     2341 cctgaacgtc tggtatggcg gg
//
LOCUS       DATCBJ010000608         4923 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_91476, whole genome shotgun sequence.
ACCESSION   DATCBJ010000608 DATCBJ010000000
VERSION     DATCBJ010000608.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 4923)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4923)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4923
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_91476"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            78..680
                     /locus_tag="VII50_12220"
     CDS             78..680
                     /locus_tag="VII50_12220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016435521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM165/GDT1 family protein"
                     /protein_id="HEY5051658.1"
                     /translation="MSLAVAAIAFGVVFLAELPDKSMVASLVLGTRYRPLYVWVGAAA
                     AFAVHVVIAVAAGGALALLPHRLVQSIVAVLFLAGAALLLFGNQTREEEAGRDESGNV
                     VEIAVPTLRRVASVSFVVIFVGEWGDITQIATANLAAKYHDPIGVGVGALLGLWTVMA
                     IGVVAGSKLVERVPLALVRRITGVILATLGVVSAVAAIRG"
     gene            complement(684..1052)
                     /locus_tag="VII50_12225"
     CDS             complement(684..1052)
                     /locus_tag="VII50_12225"
                     /inference="COORDINATES: protein motif:HMM:NF037170.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY5051659.1"
                     /translation="MQASVGYLVIDAIDPERLTTFWCAMLGVEVDSTIGEGQFVVLAP
                     TKDGLTVGFQKVPEHKTTKNRVHLDLVVEDLDAATSEIEALGGRWLEPDTTRDLEGFQ
                     WRIMADPEGNEFDIDLLPPD"
     gene            1167..2069
                     /locus_tag="VII50_12230"
     CDS             1167..2069
                     /locus_tag="VII50_12230"
                     /inference="COORDINATES: protein motif:HMM:NF013324.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HEY5051660.1"
                     /translation="MTTDSTITAEPVGHQGARVDITQRPALAITGWLGLAVAGGCIWW
                     AVVTAHHDPGLLWLPLLLCALVITSLVIVTPGQTSVVQFFGRYVGTVRRPGFWWVLPL
                     TLRRRVSIRVRNFETNHLKVNDADGNPVEVAAIVVWQVADTAKSTYAVDNFLSFVTVQ
                     AESALRHVATTHPYDDTTGTGTSLRGSTDIVAAQLAHEVAERVAIAGIEVVEVRISHL
                     AYSPEIAQAMLRRQQANAVVAARSRIVEGAVGMVELALARLTQNGVVSLDEERTASMV
                     SNLMVVLCGDQPPSPIVNVGSLYS"
     gene            2066..2266
                     /locus_tag="VII50_12235"
     CDS             2066..2266
                     /locus_tag="VII50_12235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5051661.1"
                     /translation="MSARRQVLLRVDPTVHDALIRWANDELRSVNAQVEMLLRRALTE
                     AGRMPKHAAPLPKRGRPRASDD"
     gene            2355..4616
                     /locus_tag="VII50_12240"
     CDS             2355..4616
                     /locus_tag="VII50_12240"
                     /EC_number="1.1.1.40"
                     /inference="COORDINATES: protein motif:HMM:NF007233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004470 - malic enzyme activity [Evidence
                     IEA];
                     GO_function: GO:0004471 - malate dehydrogenase
                     (decarboxylating) (NAD+) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent malic enzyme"
                     /protein_id="HEY5051662.1"
                     /translation="MLADEAREYHSSGRPGKIEIAITKSCATQHDLALAYTPGVAVPV
                     QDIALEPLAAFQYTARGNLVAVVTNGTAVLGLGNVGPLASKPVMEGKALLFKRFADVD
                     VFDIELDAADTEDFVRVVKALEPTFGGINLEDISAPQCFEIEERLRATMDIPVFHDDQ
                     HGTAIISGAALLNALELAEKSIAEVAMVCNGAGAAAIACCEFFVSLGMRRENIVLCDT
                     KGVVFRGRTDGMNRYKERFASATSARTLAEAVASADVFLGVSVAGTMTKEMVQSMADR
                     PIVFALANPDPEITYPDAKAARADVIMATGRSDYPNQVNNVLGFPFIFRGALDVRATA
                     IDDGMKVAAARALAELAKEDVPDSVLRAYGVDRLEFGPEYLIPKPLDPRVPLRVAPAV
                     VAAAMESGVARVRIDVQVYTDALANRLTRGREVMSVVSRKAQTGPKRIVFSEGEERKI
                     IRAAARIVEDGIGIPILLGRDEVIRATMDEARISAQIQIVDPARFDDIERYAEALHEK
                     RQRKGITRREAEVLLLQPNYFGAMMVEMGDADGFVSGLTYHYPDVIRPALEVIGTAAG
                     VSRAAGLYLMISEDGRAVVLTDPTVNFDPSAEELADIAVMAADRARAFGLEPRVALLS
                     FSNFGSTRHLRSAKMAAATALVKQRRPDLMADGELMADVALSPQLRSDDYPFSTLTGE
                     ANVLVFPSLEAANIAYKLLQHLGRAVAIGPILVGMAKPVHVLSRGAEVTDIVNIAAIA
                     VVDAQDASVAQPR"
     gene            4730..>4923
                     /locus_tag="VII50_12245"
     CDS             4730..>4923
                     /locus_tag="VII50_12245"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009078001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase"
                     /protein_id="HEY5051663.1"
                     /translation="MADTTERAVLAGGCFWGMQDLIRRRPGVISTRVGYSGGDVANAT
                     YRKHGSHAEALEIIFDPTQTS"
BASE COUNT          832 a         1519 c         1664 g          908 t
ORIGIN      
        1 gtgatcacca cagtcgggcg tccgcggcgc gcgactaggc ttggcacaat ggctgacagc
       61 acccggagcg actcgtcatg agcctggccg tcgccgcgat cgccttcggg gtggtctttc
      121 tcgccgagtt gcctgacaag agcatggtcg cctcgctggt gctcgggacc cggtaccggc
      181 cgctctatgt atgggtggga gcggcggctg ccttcgccgt ccatgtcgtc atcgccgtgg
      241 ctgcgggtgg tgccttagcg ctgctgccac accggttggt gcagagcatc gtggcggtgc
      301 tgttcctggc gggtgctgct ctgttgctgt tcggcaacca gacgcgcgag gaggaagcgg
      361 gccgcgacga gagcggcaac gtcgttgaga tcgcggttcc gacgctgcgc cgggtcgcct
      421 ctgtgagctt tgtggtgatc ttcgtcggcg agtggggtga catcacccag atcgcaacgg
      481 ccaacctcgc ggccaagtac cacgacccga tcggcgtcgg cgtcggcgcc ttgctcggct
      541 tgtggactgt gatggctatc ggcgttgtgg ccgggtcgaa gttggtcgag cgagtgccgc
      601 tagctttggt ccgccggatc accggagtga tcctggcgac gcttggcgtg gtgagcgcgg
      661 tggcggctat ccggggctga cggtcagtcg ggcggcagca ggtcgatgtc gaactcgttg
      721 ccttcggggt cggccatgat gcgccactga aagccctcga gatcccgcgt ggtgtctggc
      781 tcaagccagc gaccgccaag cgcctcgatc tctgaggtgg cggcgtcgag atcctcgacc
      841 accagatcca gatgcaccct gttcttggtc gtcttgtgct ccggcacctt ttggaacccg
      901 acggtcaggc cgtccttggt cggtgccagc acgacgaact gaccctcgcc gatggtgctg
      961 tcgacctcga cgccaagcat ggcgcaccaa aacgtggtca gccgctcggg gtcgatcgcg
     1021 tcgatgacga ggtagcccac cgaagcctgc atcggtcgcc tcttaatcag ttggtgccgc
     1081 gttgtttctg gatggtagac ggaggtagcg ggtcgcggca aaaagtgata gctattagat
     1141 atcactttct aagcgaggag cacgtcatga ccaccgactc aacgatcacc gccgaacccg
     1201 tcgggcacca gggagcccgc gtcgacatca ctcagcgacc cgccctcgcg atcaccgggt
     1261 ggctggggct cgcggtggcc ggcggatgca tctggtgggc ggtggtgact gcccaccacg
     1321 accccggttt gctgtggctg ccgctgctcc tctgcgcgtt ggtcatcacc tcgctcgtga
     1381 tcgtgacacc ggggcagacc tccgtggtcc agttcttcgg ccgctacgtg ggaactgttc
     1441 ggcgccctgg attctggtgg gtgctgccgc tgacgctgcg acggagagtc agcatccggg
     1501 tgcgcaactt cgagacgaac cacctgaaag tcaacgacgc cgacggcaac cccgtggagg
     1561 tcgcagccat cgtggtctgg caggtcgcgg acactgcgaa gtcgacgtac gcggtggaca
     1621 acttcttgag cttcgtcacg gtgcaggcgg agtccgcgct gcgccacgtc gcgaccaccc
     1681 acccatacga cgacacgacg ggcactggga cgtcgcttcg tggctcgacc gacatcgtcg
     1741 ccgcgcagct cgctcacgag gtcgccgaga gagtggctat cgccggcatc gaggttgtcg
     1801 aggtccggat ctcccacctc gcatactcac cggagatcgc gcaggcgatg ttgcggcgcc
     1861 agcaggccaa cgccgttgtc gcggcgcgct cgcggatcgt ggaaggcgcg gtcggcatgg
     1921 tcgagctggc gcttgccagg cttacccaga acggcgtcgt gtctttagac gaggagcgca
     1981 cagcaagcat ggtgagcaac ctgatggtcg tgctgtgcgg ggatcagccg ccgtcgccga
     2041 tcgtgaacgt cggttcgttg tattcgtgag cgcgcgtcgg caggtcctgc tgcgcgtcga
     2101 tcccacagtc catgacgcgc tgatccgctg ggctaatgac gagctgcgca gcgtcaacgc
     2161 ccaggtggag atgctgctgc gccgagcgtt gaccgaggcc ggccggatgc ccaaacatgc
     2221 ggcgccgctg cccaagcgcg ggcggccccg agcaagcgac gactaggtgc ttcgaggccg
     2281 ctcgagggca ttggcgcgcc gtattgttct ttcagatcgc gcgaatgggg caccactacc
     2341 gaacggacat gcgcatgctg gcagacgagg cccgggagta ccacagttcg ggacgccccg
     2401 gcaagatcga gattgcgatc acgaagtcat gcgcgacgca gcacgacctc gcgctggcct
     2461 acacgcccgg ggtcgcggtc ccggttcagg acatcgcgct tgaacccttg gccgcgttcc
     2521 agtacacggc acgcggaaac ctcgttgcag ttgtgactaa cggaactgcc gtgttggggc
     2581 tcggcaacgt cggaccgctc gctagcaagc cggtcatgga ggggaaagcg ctgctgttca
     2641 aacgctttgc cgacgtcgac gttttcgaca tcgagctcga tgccgccgac actgaagact
     2701 tcgttcgggt cgtcaaggct ctggagccga cgttcggcgg catcaacctc gaagacatca
     2761 gcgcgccgca gtgctttgag atcgaggaac ggctgcgcgc gacgatggac attcccgtct
     2821 tccacgacga ccagcacggg acggcgatta tcagcggcgc ggcattgctc aacgcgctcg
     2881 agttggcgga gaagtccatc gccgaggtcg ccatggtgtg caacggtgcc ggcgctgcgg
     2941 cgatcgcgtg ctgcgagttc ttcgtctcgc taggtatgcg cagggagaac atcgtcctct
     3001 gcgatacgaa aggtgttgtc ttccgcggtc gcacagacgg aatgaaccgc tacaaggaac
     3061 gattcgcctc cgctacgtcg gcgcgaacgt tagccgaagc cgtggcctcc gctgatgtat
     3121 tcctcggggt gtctgtcgcc ggcacgatga cgaaggagat ggtgcagtcg atggccgatc
     3181 ggccaatcgt cttcgccctg gccaatcccg atccggagat cacctatccc gatgcgaaag
     3241 cggcgcgcgc cgacgtcatc atggcgaccg gacgctccga ctatcccaat caagtgaaca
     3301 acgtcttggg tttccccttc atcttccgag gtgcgctcga cgttcgggcc accgcaattg
     3361 atgacggcat gaaggtggca gcagcgaggg cgctcgccga gcttgcgaag gaagacgttc
     3421 cggattccgt gcttcgtgcg tacggcgtcg accgtctcga gttcgggccg gagtacctga
     3481 tccccaagcc gctcgatcca agggttccgt tgcgcgtggc gccggcggtg gtcgccgccg
     3541 cgatggaatc tggtgtggcc cgagtgcgga tcgacgtgca ggtctacacc gacgcgctcg
     3601 ccaaccggct gacgcgtggc cgtgaagtga tgagcgttgt cagccggaaa gcgcagactg
     3661 gccccaagcg gatcgtgttc tctgagggcg aggagcgcaa gatcatccgc gcggcggcgc
     3721 gcatcgtcga agacgggatc ggtatcccca tcctgttggg ccgagacgaa gtgatccgcg
     3781 cgacgatgga tgaggcgcga atcagcgcac agatccagat cgtcgacccg gcgcgctttg
     3841 acgacatcga gcggtatgca gaggcgcttc acgagaagcg gcaacgcaag ggaatcacga
     3901 ggcgcgaggc cgaggtgttg ctgctccagc cgaactactt cggcgcgatg atggtcgaga
     3961 tgggggacgc cgacgggttc gtgtctggcc tcacgtacca ctatcccgac gtcatccgtc
     4021 ctgccctgga ggtgattggt acggcggcgg gagtgtctcg tgccgcaggt ctgtatctga
     4081 tgatcagcga agacggccgt gcagtcgttc ttacggaccc gaccgtgaac ttcgacccgt
     4141 ccgcggaaga gctcgcggac atcgcggtga tggctgctga tcgcgcacga gcattcggcc
     4201 tggagccacg cgtggcgctg ctctcgttct cgaacttcgg atcgacccgt catctgaggt
     4261 cggcgaagat ggccgccgcg acggcgctgg tgaagcagcg ccgtccggat ctgatggccg
     4321 acggcgaact catggccgac gtcgcgctct cgcctcaact gcgcagcgac gactacccgt
     4381 tctccaccct cactggggaa gcgaacgtcc tcgtctttcc cagtcttgag gcagcgaaca
     4441 tcgcctacaa gctgttgcag cacctcgggc gcgcggtcgc gatcggcccg attcttgtcg
     4501 gcatggcgaa gccggtgcac gtgctgagcc ggggtgccga ggtcaccgac atcgtcaaca
     4561 ttgcggccat cgcggtggtc gatgctcagg acgcgagcgt ggctcagccc cgctgatctc
     4621 ccgcaaacat cgcagcaggg cgcgaggaaa tggcgggaag cttcgatagc taaacttggt
     4681 tgcatagtcg agatgcgttg acgctggcgg acggatagga gcattggcga tggctgacac
     4741 tactgagcgg gctgtcttgg ctggcgggtg cttttggggg atgcaggacc tcattcgccg
     4801 ccgtccgggc gtgatctcga cccgagtcgg ctacagcggt ggcgacgtgg cgaacgcgac
     4861 ctaccgtaag cacggatccc atgcggaggc gctcgagatc atcttcgatc cgacgcagac
     4921 ctc
//
LOCUS       DATCBJ010000609         2146 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_165726, whole genome shotgun sequence.
ACCESSION   DATCBJ010000609 DATCBJ010000000
VERSION     DATCBJ010000609.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2146)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2146)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2146
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_165726"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1225)
                     /locus_tag="VII50_12250"
     CDS             complement(<1..1225)
                     /locus_tag="VII50_12250"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY5051664.1"
                     /translation="MTDRTTFREAEESLRKSEAQYRQIVEATSDGIIQLDERRTVVFV
                     NQRLADMLGYDASEMIGRDFLDFVSSAAQTTFSNSLAERKAGRKQPVDTIYCHRDGRS
                     ISVSVAGAATFDAEGRFSGTLGLVRDMTERNKLQSQLMVSDRMASIGTLAAGVAHEIN
                     NPLAAVLANLDFIAESMSASVQGSIAATSPASSRPWLCGEIQGPLDDAREAARRMRFV
                     VRDLKVFSHPASDDLRAPVNVRALMDSSLRMAENEIRHRARVVKQYGPVPPLDINEAR
                     IGQVFLNLLVNAAQALPEDHADNNEIHVDIRLDGAWVIIEVRDTGSGIPPEIIGRIFD
                     AFFTTKAVGVGTGLGLAISHRIVTDMGGDLTVESTMGTGTLFRVSLPVTRAVDVAVAT
                     IVEPPPAPKVRGHILV"
     gene            1385..1567
                     /locus_tag="VII50_12255"
     CDS             1385..1567
                     /locus_tag="VII50_12255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5051665.1"
                     /translation="MAVGDKPLERTADHLGNRVAEQFGDSTVGEREHPIDIDGADAFI
                     GRFDDAAIAFLALAKR"
     gene            complement(1711..1965)
                     /locus_tag="VII50_12260"
     CDS             complement(1711..1965)
                     /locus_tag="VII50_12260"
                     /inference="COORDINATES: protein motif:HMM:NF019309.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="circadian clock KaiB family protein"
                     /protein_id="HEY5051666.1"
                     /translation="MRLYIAGNAPNSLDAVANARAICEEHFPAAHELEIVDLLVHPQR
                     AAIDGIIVTPTLLKLSPPPVQRMIGSLSDAHEVMRMLADP"
BASE COUNT          333 a          702 c          712 g          399 t
ORIGIN      
        1 ccaccagaat gtggccgcgc accttcggcg ccggcggcgg ctccacgatg gttgccaccg
       61 ccacgtccac cgcgcgcgtg actggaagcg acacacgaaa gagcgttccc gttcccatgg
      121 tgctctcgac tgtcagatcg ccacccatat ccgtgacgat gcgatggctg atggcgaggc
      181 cgaggccggt gccgacgcct acggccttcg tcgtgaagaa cgcatcgaag atccggccga
      241 taatttctgg cggaattccc gagcccgtgt cgcgcacctc gatgatgacc catgcgccgt
      301 cgagccggat gtcgacgtga atctcgttgt tatcggcgtg gtcttcgggc agcgcttgcg
      361 ccgcattgac cagcaggttg agaaacacct gtccgatgcg cgcctcgttg atgtcgagcg
      421 gaggcacggg gccgtactgc ttgaccacgc gtgcgcgatg ccgaatctcg ttttcggcca
      481 tgcgcagcga cgagtccatc agcgccctca cgttcaccgg agcgcgcagg tcgtcgctgg
      541 ctgggtgcga gaacaccttg agatcgcgca cgacgaaccg catgcgcctg gcggcttcgc
      601 gtgcgtcgtc gaggggtcct tggatctccc cgcacagcca ggggcgggac gaggccgggg
      661 aagttgcggc gatgctgccc tgcaccgacg cgctcatgct ctcagcaata aagtccagat
      721 tcgcgagcac cgcggccagc ggattgttga tctcgtgggc gacgcccgcc gccagagtac
      781 cgatcgaggc catgcggtcg ctcaccatca gctgtgactg cagcttgttg cgttcagtca
      841 tgtcacgcac gagcccgagc gtcccggaga agcgcccctc cgcgtcgaag gtcgcggcgc
      901 cggcgacgct gaccgagatg ctcctgccgt cgcggtgaca atagatcgtg tcgacgggct
      961 gcttccgccc cgccttgcgc tccgcgagcg agttcgagaa tgtcgtctgg gcggccgagc
     1021 tcacaaagtc gaggaagtcc cggccgatca tctcgctcgc gtcgtagccg agcatatcgg
     1081 cgagccgctg attcacgaag acgaccgtgc gccgctcgtc gagctggatg atgccgtcgc
     1141 tcgtggcttc gacgatctgg cggtactgtg cttcgctctt gcgcagcgac tcctccgctt
     1201 cgcggaaggt cgtacggtcg gtcatcatgc cgagcgtacc gacgatcgtt ccgtcggcgt
     1261 cgcgaatcgg cgtggtgcgg agccgtaccc acagcgcgct gccgtctcgg cgtcggtaca
     1321 cgacctcctg ctcttccgat attccctcgt gtgagcgctc gcgccggagc ctcgtgatcg
     1381 cttcgtggcc gtcggggaca aaccgctcga gcggactgcc gatcatctcg gcaaccgagt
     1441 agccgagcaa ttcggcgatt cgacggttgg cgaacgtgaa catcccattg atatcgatgg
     1501 tgcagatgcc ttcatcggcc gtttcgacga tgcggcgata gcgttcctcg ctctcgcgaa
     1561 gcgctgactg cgcgttggag agcagcaggg gcgtctggct cgatgcgtcg accaccgcgt
     1621 cgatctcacc cgagaccagt gcgtggatgg tcgccgcggc ttcggcgagt tgccgctcga
     1681 ggtcgtggac atgtcggacg ctcgtgacgc tcatgggtcc gccagcatgc gcatgacctc
     1741 gtgcgcgtcg ctgaggcttc cgatcatgcg ctgcacaggg ggtggcgaga gcttgaggag
     1801 cgtcggcgtc acgatgatcc cgtcgatcgc ggcacgctgc gggtgcacca gcaagtcgac
     1861 gatctcgagt tcgtgcgcgg ccgggaaatg ctcttcgcag atcgccctgg cattggcgac
     1921 ggcgtccaac gaattcggcg cattccccgc gatgtaaagg cgcaaccgaa gccgcggcgg
     1981 tttttggtgc agcgccctgg gcatcctacc gcgctcggcg acggtcggcg ggcgcctcgc
     2041 gatgcggtgc gccctttcgc ttggcgcggc cgacctccgc gtcggcgtgc cgcatcttgc
     2101 ggcgtaccgc gcggtcggac ttcaggcgcg tggatgccaa acccgt
//
LOCUS       DATCBJ010000610         3743 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_202217, whole genome shotgun sequence.
ACCESSION   DATCBJ010000610 DATCBJ010000000
VERSION     DATCBJ010000610.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 3743)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3743)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3743
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_202217"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            296..730
                     /locus_tag="VII50_12265"
     CDS             296..730
                     /locus_tag="VII50_12265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016826463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3052 domain-containing protein"
                     /protein_id="HEY5051667.1"
                     /translation="MSATADHAEAGRTRAEKLGFQAGQVVQEIGYDDDCDEDLRASIE
                     KTIGSELVDEDYDDVIDAVVLWWREDDGDLVDALVDALTSLADGGTIWLLSPKAGREG
                     HIEPSDVTEAAPTAGLSQTSSISAGPDWSGARLVAPKSSRKR"
     gene            727..1236
                     /locus_tag="VII50_12270"
     CDS             727..1236
                     /locus_tag="VII50_12270"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HEY5051668.1"
                     /translation="MTAAPDSLPDGLVPDAGLPAVGAPAPDFELVDQHGTPVRLSSFR
                     GMRNVLLVFYPWSFSRTCTGELCVLRDDLASFQNDDAQLLAISIDSKHVQRQFAEREG
                     FEFPLLADFWPHGEVARRYGVFDEVKGVALRGTFVIDRFGIVRWSVLRDIPDARDPDE
                     YRRVLAELR"
     gene            1290..1361
                     /locus_tag="VII50_12275"
     tRNA            1290..1361
                     /locus_tag="VII50_12275"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1322..1324,aa:Val,seq:tac)
     gene            1450..1776
                     /locus_tag="VII50_12280"
     CDS             1450..1776
                     /locus_tag="VII50_12280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5051669.1"
                     /translation="MRIGFSVFLMAVGAVLTFAVHKTATGVNIHTIGVILMIAGVIGL
                     AATLTIFAPRRGVSAVATERTVTTAPAAAPVAMPVAAPAATPAVVTTTPAGVTTQQVV
                     EHREVY"
     gene            2040..2306
                     /locus_tag="VII50_12285"
     CDS             2040..2306
                     /locus_tag="VII50_12285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5051670.1"
                     /translation="MPTVIGHHDVKDKDHWLASPKREEVFGPLGVTNIRTFVNPQNPT
                     QVAVLMDVADLDAMMKAMQEDPAMGEAMAFDGVLPETLVILVEA"
     gene            2334..3089
                     /locus_tag="VII50_12290"
     CDS             2334..3089
                     /locus_tag="VII50_12290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011553803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase subunit A"
                     /protein_id="HEY5051671.1"
                     /translation="MDKVVPSAEQAVADVFDGASIAIGGFGLCGVPSVLIDALLEQGA
                     TNLETVSNNCGVDGLGLGVLLEAHRIRRHTGSYVGENQEYERQYLTGVLEVEFSPQGT
                     LAERLRAGGAGIPAFFTPSGIGTQVADGGIPRRYHPDGTIAVLSEPKEVRDFDGVSYV
                     LERGIATDFAFVHAWKGDRHGNLVYRKTARNFNPLCAAAGRITIAEVELLLEPGEIDA
                     DEVDTPGIFVHRVVHTPDVVKHIERRTTTGKGA"
     gene            3091..>3743
                     /locus_tag="VII50_12295"
     CDS             3091..>3743
                     /locus_tag="VII50_12295"
                     /inference="COORDINATES: protein motif:HMM:TIGR02428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacid CoA-transferase subunit B"
                     /protein_id="HEY5051672.1"
                     /translation="MAFDRGGLAARVAAELHDGQYVNLGIGMPTLVSNHLPDGVTVVL
                     QSENGILGTGPYPALDEVDADLINAGKETVTVLPGASFFDSAASFGMIRGGHIDVAVL
                     GAMQVSAQGDLANWMIPGKMVKGMGGAMDLVHGAKRVIVMMEHRARDGSSKILKHCTL
                     PLTGRGCVDRVITDLAVIDVTADGLLLVETAPGVSVDEVDADLINAGKETVTVLPGA"
BASE COUNT          627 a         1182 c         1247 g          687 t
ORIGIN      
        1 ctgatgaccg cggggcggtc tcctgctctg cctacctggg ccacgggaat cctcacgccg
       61 gaactgtctc ggacacgcgt ggggattgtc tcatcacttc gctctaccct gcgttgcgct
      121 atgtttccgg cccgcttgcg ctgcggtttc cgggcgaagg catctgttgt ttacagacca
      181 gcacttgcgc gcggggcccc ggtgcgttgg actacatcga ctgagccctg ccacggggcc
      241 cagcagtagg cgccgtcgcg gtgtcgacag cacggcaacg caggggagga tcttcgtgag
      301 cgcgaccgcg gaccacgcgg aggctggacg tacccgggcg gagaagctcg gcttccaggc
      361 ggggcaggtg gtccaggaga tcggctacga cgacgactgc gacgaggacc tgcgtgcgtc
      421 gatcgagaag acgatcggca gcgaacttgt cgacgaggac tatgacgacg tcatcgacgc
      481 cgtggtcctt tggtggcgtg aagacgacgg cgacctggtc gacgcgctcg tcgacgcgct
      541 gacctcgttg gctgacggcg gcacgatctg gctgctgtct cccaaggccg gccgcgaggg
      601 ccacatcgag ccgagtgatg tcacggaggc cgcgccgacc gctggtctga gccagacgag
      661 ttcgatcagc gctggtcctg actggtccgg tgcccgcctc gtcgcgccga agagttctcg
      721 caagcgttga ctgctgcacc ggattcactg cccgacggtt tagtgccgga cgccgggttg
      781 ccggcggtgg gcgcgccggc accggatttc gaactcgtcg atcagcacgg cactccggtg
      841 cggctttcgt cgtttcgggg tatgcgcaac gtccttctcg tcttctatcc ctggtcgttc
      901 tcgcgtacgt gcacgggcga gctgtgcgtc ttgcgcgacg acctcgcgtc gttccaaaac
      961 gacgacgccc agttgctggc gatctcgatc gattcgaagc acgtgcagcg ccagttcgcg
     1021 gagcgcgagg gattcgagtt cccgctgctc gccgacttct ggccccatgg cgaggtggcg
     1081 cgtcgctacg gcgtcttcga cgaggtgaaa ggcgtggcgt tgcgtgggac cttcgtcatc
     1141 gaccgcttcg gcatcgtgcg ctggtcggtg ctgcgcgaca tcccggacgc acgcgacccg
     1201 gacgagtacc gccgcgtcct cgccgaactg cgctaaaccg cgcgcatcgg taggctgacc
     1261 ggcgcgctgc ttgtgcgtcg ggcagccgcg ggcgcgtagc tcagcggaag agcacccggc
     1321 ttacacccgg gcggtcacag gttcgaaccc tgtcgcgccc acctggtggt ctgcgcgttt
     1381 caccggcggg ggcgtcgggt aattgcgctg tgcacggctg cgccgcagcc aattcactgg
     1441 agaatcctca tgcggatcgg tttctcggtc tttctgatgg ccgttggtgc agtcctgacc
     1501 ttcgctgtac acaagaccgc gacaggcgtg aatatccaca cgatcggcgt catcttgatg
     1561 atcgccggcg tcattggtct cgcagccacg ttgacgatct tcgcgccacg caggggcgtt
     1621 tccgcggtgg ccaccgaacg gactgtcacc acggcccctg ctgcggctcc cgttgcaatg
     1681 cccgtcgcgg ctcccgctgc gaccccggca gtggtgacca caaccccggc tggcgtgacc
     1741 acccagcagg ttgtcgaaca tcgagaggtc tactaaggat ctgggggaca tagcgtgacc
     1801 ccatgggctt cgttacccgc accgtccgct acgtgctgac gccgcgtgcg atacgtcgcg
     1861 cgatacgagc taggcaatcg gtcgtgccgc gacgggttcg gccgtcattg ttcaggcgcc
     1921 gtcgttagca agacgggcca cgggacgagc ggtcggtctg ttcgtcacta cgaagtaggc
     1981 ctacgatccg cgggacgggt ccgccgaagg tgctggaccc actaccgcga ggagtcggca
     2041 tgcccacagt catcggtcac cacgatgtga aggacaagga ccactggctg gcttcgccca
     2101 agcgtgaaga ggtgttcgga cctctcggcg tcaccaacat ccgcacgttc gtcaacccgc
     2161 agaatccgac ccaagtggcg gtcctgatgg acgtcgcgga cctggacgcc atgatgaagg
     2221 cgatgcagga ggacccggcc atgggcgagg cgatggcgtt cgacggcgtg ctgcctgaga
     2281 ccctggtgat cctcgtcgag gcctagaacc ttgggacgtg accggaggat ttcgtggaca
     2341 aggtagtgcc ttcggctgag caagccgtgg cggatgtctt cgacggcgcg tcgatcgcga
     2401 tcggtggatt cggcttgtgc ggagtcccct cggtgctgat cgacgcgctg ttggaacaag
     2461 gcgcgaccaa cctggagact gtaagcaaca actgcggcgt cgacggtctc gggctcgggg
     2521 tgttgctcga ggcgcaccgg attcgccggc acaccggctc gtatgtcgga gagaaccagg
     2581 agtatgagcg gcagtacctc accggtgtgc tcgaggtcga gttcagcccg cagggaacgt
     2641 tggccgaacg gctgcgcgca ggcggtgcgg gcattcctgc cttcttcacg ccatcaggca
     2701 tcggcaccca ggtagcggac ggcggtatcc ctcgccgtta tcaccctgac ggcaccatcg
     2761 cggtgctgag cgaacccaaa gaggtgcgcg acttcgacgg tgtgagctac gtgctcgaac
     2821 gtggcatcgc caccgacttt gcgtttgtgc atgcatggaa aggcgaccgg cacggcaacc
     2881 tcgtgtatcg aaagaccgcg cgcaacttca acccgctctg cgcagcagcc ggacgtatca
     2941 cgatcgccga agtcgagctg ctgcttgagc ctggcgagat cgacgccgat gaagtcgaca
     3001 cccccggcat cttcgtacat cgggtcgtgc acacgcccga cgtcgttaag cacatcgagc
     3061 gtcgtaccac cactggcaag ggtgcctgag atggcattcg atcgtggcgg tctcgcggcg
     3121 agagttgccg ccgaactcca tgacggccaa tacgtgaacc tcgggattgg catgccgaca
     3181 cttgtgtcga atcacttgcc cgacggcgtc actgtcgtct tgcagagcga gaacggcatc
     3241 cttggcaccg ggccgtatcc agccctcgac gaggtcgatg ccgatctcat caacgcgggc
     3301 aaggaaaccg tcacggttct gcccggcgcg agcttcttcg attccgcggc ctcgttcggc
     3361 atgatccgcg gcggacacat tgatgtcgcg gtgctcggtg cgatgcaggt cagcgcgcag
     3421 ggtgatctcg ccaactggat gatccccggc aagatggtca agggcatggg cggcgcgatg
     3481 gatctcgtcc acggcgcgaa gcgcgtcatc gtgatgatgg aacaccgcgc gagggacggc
     3541 agctcgaaga tcctcaagca ctgcacattg ccgctgacgg gccgaggctg cgtcgatcgc
     3601 gtcatcaccg atttggcggt catcgacgtc acggccgacg gtctgcttct cgttgagaca
     3661 gcccccggcg tcagtgtcga cgaggtcgat gccgatctca tcaacgcggg caaggaaacc
     3721 gtcacggttc tgcccggcgc gag
//
LOCUS       DATCBJ010000611         2275 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_17588, whole genome shotgun sequence.
ACCESSION   DATCBJ010000611 DATCBJ010000000
VERSION     DATCBJ010000611.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2275)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2275)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2275
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_17588"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            511..1899
                     /locus_tag="VII50_12300"
     CDS             511..1899
                     /locus_tag="VII50_12300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5051673.1"
                     /translation="MGLATLDIATPDVAAAPRRGMPVAALLLLAALAAGVRAQGAFYV
                     AGQLVVGVLIAAATVAAMRSSSFRGRDFGLPGVFAAMLAVWSIASDAVARHVGAAAPG
                     VALLAAILATFAIVARLDATQRGWFVDALTVIGVVVGLSGWVGVAAHVTPLAHVDQGL
                     WRAATTLTYTNAAAGLLMPMAFVAVARLADFVARRERALRSAAVTVLWMSIGATFSRG
                     GVVAVLVGLIVVAFAADRRALARALVGPAIGASIAIAGMLVSVPQNSHPHVVAASALL
                     TVGIGIAVAIDSLGTWRGTAAVVGALALAVVVAIAASPSIRHATTTIAHPRLTATSDD
                     RVRESSTVLREIEQRSVFGAGGGSQTLQWSAPDGSVLFDRYAHDEYLQVAWKSGVVGL
                     GLLLAMLGAFAARIRRGRREARSALWVGPVAGLCGLAASSALDFLWHIPVIPLVGAVL
                     AGLCAPTKGNSL"
     gene            1896..>2275
                     /locus_tag="VII50_12305"
     CDS             1896..>2275
                     /locus_tag="VII50_12305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5051674.1"
                     /translation="MSKRMAAGIAVAIVSGSLMSGAVPALGAVTNSSPPTQVQAHLGN
                     PDKISARGAAVDVLLTVKCPRTSGVADVSVQVQQQNRNGLAGSYTMMPAFVTCTGGKE
                     TVDIYVQAVDHPYEAGPATVTAQVV"
BASE COUNT          317 a          743 c          792 g          423 t
ORIGIN      
        1 ccgcccgcta gtttgccgcc gaaccgctcc gaggccggca ccttgaggtt cgtgctcgcc
       61 ttcgtccacg tcctcttgcc ttcgctgccg tgcgcgaaag cgacacacac tgcccgacgc
      121 taggaaccgg cgcgtccgag gacagagatc cggatgtgat cgaggacgag ctgctggatg
      181 gccctacgca cagctgcgag ccggacacgg tcgagaccct gcccgtgaaa caacggcctt
      241 cgccgtcgcc acagctgaca gccacgcaaa gaagtagtgc gactccaccg ccggccaagc
      301 aagccatccg aatcggcacg gcagcctcct cgctgctcga agggcgtaag cccggcccgg
      361 gcggctagcc gagccaagta catccggtaa cgctgcgagg tgcggtgcta tgggggcgcg
      421 ctgacaacca tccgtcgagt gtttgcgtca ccccagtcga ccgacagttc cggcccgcgg
      481 ccgcgactgt cacgacatga gggggacggc gtggggctgg cgacgcttga catcgcgacg
      541 cccgatgtcg cagctgctcc ccgccggggg atgccggttg cggccctgct gttgcttgcc
      601 gcgctcgccg ccggcgtgcg agctcagggc gcgttctacg tcgccgggca gctggtcgtc
      661 ggcgtattga tcgcggcggc gaccgttgcg gcgatgcgat cgtcgtcctt tcggggtcgc
      721 gacttcggct taccgggcgt cttcgcggcg atgctcgcgg tgtggtcgat cgccagcgat
      781 gccgtcgcgc gtcatgtcgg cgctgctgcg ccgggggtgg cgctacttgc cgcgattctg
      841 gcgacgttcg cgattgtcgc gcgtctcgac gcgacgcagc gcggctggtt cgtcgatgca
      901 ttaacggtca tcggcgttgt tgtcggcttg tcgggttggg ttggcgtcgc cgcccacgtg
      961 acgccactcg cgcacgtcga tcaaggattg tggcgagcgg cgacaacgct gacgtacacc
     1021 aacgcggccg ccgggctatt aatgccgatg gccttcgtcg ccgtggcgcg cctcgcggat
     1081 ttcgttgcac ggcgcgagag ggcgctgcgg tcggctgccg tcacggtgct ctggatgagc
     1141 atcggcgcga cgtttagccg gggcggggtc gtggcggtgc tggtaggtct catcgtcgtt
     1201 gcgttcgccg cggatcgacg cgcccttgct cgagcgctcg tcgggccggc gatcggcgcg
     1261 agtatcgcga tcgccgggat gctggtgagc gttccgcaga attcccaccc gcatgtggtt
     1321 gcggcgtccg cgttgctgac tgtcggcatt ggcatcgctg tggcgatcga ctctctcggc
     1381 acctggcgcg gaacggctgc ggtagtcggc gccctcgccc tcgctgtcgt cgtcgcgatt
     1441 gcggcgtcgc cctcgatacg gcacgcgacg accactatcg cgcatccgcg gcttactgcg
     1501 acgtccgatg accgcgtgcg cgaatcgtcg accgtcctgc gcgagatcga gcagcggtca
     1561 gtgttcggtg ctggcggggg ttcgcaaacc ttgcaatgga gcgcacccga cggcagcgtc
     1621 ttgttcgatc gttacgccca cgacgaatac cttcaggtcg cgtggaagtc cggcgtcgtc
     1681 ggcctcggtt tgttgctggc gatgttaggc gcgttcgccg cgcgtatccg tcgcgggcgt
     1741 cgtgaggcga ggtcggcctt gtgggtcggc ccagtagcgg gcctttgcgg actcgccgcc
     1801 tcgagtgcgc tcgacttctt gtggcacatc cccgtcattc ccctggtcgg cgccgtgctg
     1861 gctgggctgt gtgcaccaac gaagggaaac tccctatgag taagcgcatg gcggcaggga
     1921 tcgccgtcgc cattgtgagc ggatcgctga tgtccggtgc ggtaccggcg cttggggcgg
     1981 tgaccaacag ctcgccgccg acccaggttc aggcgcacct gggtaacccg gacaagatct
     2041 ccgcccgcgg tgctgcagtc gacgtgttgc tcaccgtgaa gtgccctcgg acatcaggtg
     2101 tcgccgacgt aagcgtgcag gtgcagcaac agaaccgcaa cggtctcgcg ggtagctaca
     2161 cgatgatgcc cgcgttcgtc acgtgcactg gcggcaagga aacggtcgac atctacgtcc
     2221 aggccgtcga tcacccctac gaggccggac cggcgacagt cacggcgcag gtcgt
//
LOCUS       DATCBJ010000612         4561 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_202253, whole genome shotgun sequence.
ACCESSION   DATCBJ010000612 DATCBJ010000000
VERSION     DATCBJ010000612.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 4561)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4561)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4561
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_202253"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..338)
                     /locus_tag="VII50_12310"
     CDS             complement(<1..338)
                     /locus_tag="VII50_12310"
                     /inference="COORDINATES: protein motif:HMM:NF014619.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="laccase domain-containing protein"
                     /protein_id="HEY5051675.1"
                     /translation="MLWLGSLGGDVRFAFTSRLGGFSAAPYDSLNLSLNVGDDQDAVE
                     RNRRDVLERLGVPRAGWLHAQHGAVVQFVNGTDDPGSPGGGHEADGLVTRATDFALGA
                     LSADCALVVLA"
     gene            complement(339..1535)
                     /gene="ftsZ"
                     /locus_tag="VII50_12315"
     CDS             complement(339..1535)
                     /gene="ftsZ"
                     /locus_tag="VII50_12315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HEY5051676.1"
                     /translation="MAAPQNYLAVIKVVGIGGGGVNAVNRMIEVGLKGVEFIAINTDA
                     QALLMSDADVKLDIGRELTRGLGAGADPEVGRQAAEDHTEEIEEVLKGADMVFVTAGE
                     GGGTGTGGAPVVARLSRALGALTIGVVTRPFGFEGRRRGEQADVGIEELREEVDTLIV
                     IPNDRLLSISDRKISVLDAFKSADQVLLQGVSGITDLITTPGLINLDFADVKSVMSGA
                     GSALMGIGSARGEDRAVAAAEMAISSPLLEASIEGAHGVLLSVAGGSDLGLFEINEAA
                     QLVAEAAHPDANIIFGAVIDDALGDEVRVTVIAAGFDGGSPRPARQSARRVIETPRPT
                     DAIDDAINVTTAPPAATRSELREPERVSLPEAGAIPRRDPAAPRRTIVFEDEVEDELD
                     VPDFLK"
     gene            complement(1836..3275)
                     /gene="murC"
                     /locus_tag="VII50_12320"
     CDS             complement(1836..3275)
                     /gene="murC"
                     /locus_tag="VII50_12320"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HEY5051677.1"
                     /translation="MSANLRSDVVPVGELGVVHFIGIGGGGMSGIARIMLTRGTTVTG
                     SDAKEARVLAALRVLGAKVFVGHDAANLGAADTVVVSTAIQESNPELIEARTRGLRIL
                     PRAAALASVMAGRRGIAVAGTHGKTTTTSMLARALQHCGEDPSFAIGADLNEPGSNAH
                     DGVGGLFVAEADESDESFLLLSPFAAIVTNIEADHLNHYAGLAEIHQAFERFAGRIAP
                     DGFLVVCADDAGAMQLARFARRAGIDVRTYGEAVDADVRIAKLELAGTGSSFQIIAGG
                     RRLGEVALRVPGRHNALNAAAAITVGLGLDLPFAEFAAGLTVFTGARRRFEPKGVVNG
                     VRVYDDYAHHPTEIAAILRAAREVVGSGRLVVAFQAHHYYRTADFCAEFGQALGLADD
                     VVVMEVYAPGETMLPGGTGTALAAAVTLSPEHVVFEPSWSAVPAQLAERARPGDLVLT
                     MGAGGDVAMLGPEVLEALRQRAQAAAVLP"
     gene            complement(3272..4408)
                     /gene="murG"
                     /locus_tag="VII50_12325"
     CDS             complement(3272..4408)
                     /gene="murG"
                     /locus_tag="VII50_12325"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HEY5051678.1"
                     /translation="MHVVLAGGGTAGHVEPALAVADALRRRDPDVGITMLGTARGLET
                     RLVPARGYDLAFIPPVPLPRSLTPSFLTVPTRMRTAVREATAIFDNVGADVVVGFGGY
                     VALPAYLAARGAKLPFVVHEANAKPGLANRWGARFTPYVAASTPDPKLPHAQVIGVPL
                     RRAISTLDRADLRGRARAQLGLLDDVPTLLVSGGSQGARSLNRAVVGVVADLLAAGIQ
                     VLHIAGPTQVADVDAAKPEPIAGQPPYVLVPYVDQMELAYAAADLMLCRAGAMTCAEL
                     TAVGLPGIYVPLPHGNGEQRFNAAPIVDSGGGLLVSDAELRPAWIRDQVIPLLTNPRR
                     LHAMSVRAADLGRRDADEKMVDMILEASGSDADRAGRPRGGRGR"
BASE COUNT          797 a         1583 c         1483 g          698 t
ORIGIN      
        1 gccaggacga cgagggcgca gtccgccgac aaagcgccga gcgcgaaatc ggtcgcccgc
       61 gtcacgagac cgtcggcctc gtgcccgccg ccggggctgc cggggtcatc ggtgccgtta
      121 acaaactgga ctacagcgcc gtgctgagca tgcagccagc cggcgcgcgg cacccccagt
      181 cgctcgagta cgtcgcggcg attgcgttcc acggcgtcct ggtcatcccc aacgttcaag
      241 ctcaggttga ggctgtcata cggcgccgca ctaaacccgc cgagccgact ggtgaaggcg
      301 aaccgcacat caccaccgag actcccgagc cagagcacct acttcaagaa gtcggggacg
      361 tcgagttcat cttcgacctc gtcctcgaac acgatcgtgc gacgtggcgc cgcgggatca
      421 cgacgcggaa tcgcaccggc ttcaggcaac gacacgcgct ccggctctcg cagctcgctc
      481 cgggtggctg ccggcggggc ggtcgtgacg ttgatggcgt cgtcgatcgc atccgtcgga
      541 cgtggcgtct cgatgactcg acgtgccgat tggcgagccg gccggggcga gccaccatcg
      601 aatcccgcgg cgatgaccgt gacccgcacc tcgtcgccca gtgcatcgtc gatgacggcg
      661 ccgaaaatga tgttggcgtc ggggtgggct gcttcggcga cgagttgagc agcctcattg
      721 atctcgaaga gaccgaggtc tgatccgccg gctaccgaca acaagacgcc gtgcgcaccc
      781 tcgatcgacg cttcgagcaa cgggctcgag atcgccatct cggcggccgc caccgcgcgg
      841 tcttcaccgc gcgctgaacc gatccccatc aacgccgaac cggcgccgga catcaccgat
      901 ttcacgtcgg cgaagtcgag gttgatcagg cctggcgtgg tgatgagatc cgtgatgccc
      961 gagacgcctt gcagcaggac ctgatcggcc gacttgaacg cgtcgagcac gctgatcttg
     1021 cggtcactga tcgacagcaa ccggtcgtta ggaatgacga tgagggtgtc gacctcctca
     1081 cggagttctt cgatgccgac gtccgcctgc tcgccgcgac ggcgaccctc gaatccgaac
     1141 ggccgcgtaa cgacgccgat agtcagggcg ccgagtgcgc gcgaaagtcg cgcgacgacc
     1201 ggcgcgccac cggtcccggt gccgccgccc tcgccggcgg tcacgaacac catgtcggcg
     1261 cctttgagta cctcttcgat ctcctcggtg tggtcctcgg cggcctgccg gccgacctcg
     1321 gggtccgcac cagcacccaa gccgcgggtg agctcacgac cgatgtcgag tttgacatcg
     1381 gcgtcgctca tcagcagtgc ctgcgcgtcg gtgttgatgg cgatgaactc gacacccttg
     1441 agaccgactt cgatcatccg gttgacggcg ttgacacccc cgccaccgat gccgacgacc
     1501 ttgatgaccg cgaggtagtt ctgcggtgct gccacggact ttcgccttgc cttccctcgt
     1561 cggtcaaaca cattcgtgct cacgcgggca acccacccgg tgcgtgagtg caaacccatg
     1621 cgggcggcga tgcggggaag ccaccaccga cggcgagatc gcagaccgga tatccgcgac
     1681 cctcatcctc aagtagaccg ttatagttat gtcaacctga ggacgtcacg aagattagag
     1741 ccccgaaccg cgaagggtca acgcgggtag cggcgtgtcg ccgtgcgagc cggattttgt
     1801 gcgagctgtg cgagcccgac gcacaacgag cacgattagg gcaagacggc tgcggcctgt
     1861 gcgcgctgcc gcaacgcctc gagtacttcc ggcccgagca tcgcgacatc tcccccggcg
     1921 cccatcgtca acacgaggtc cccgggccgc gcgcgttctg ccagttgcgc gggcaccgcc
     1981 gaccatgacg gttcgaagac gacatgttcg ggcgacaagg tcacagccgc cgcgagtgcg
     2041 gtgccggtgc cgccgggcag catcgtctcg ccaggcgcgt agacctccat cacgacaacg
     2101 tcgtcggcca atccgagggc ctgaccgaac tcggcgcaga agtccgcggt tcggtagtag
     2161 tggtgggctt gaaaggcgac gaccaatcgc ccgctgccga cgacttcgcg ggcagcccgc
     2221 aggatcgccg cgatctcggt cggatggtgc gcgtagtcgt cgtagacccg cactccgttg
     2281 accacccctt tcggctcgaa ccgccgtcgc gctccggtga aaaccgtgag gcccgcggcg
     2341 aactcggcga aggggagatc gagaccgagg ccaacggtga tcgccgcagc tgcgttgagt
     2401 gcgttgtgcc gacccggcac ccgcaacgcg acctcaccca gtcgccgtcc gcccgcgatg
     2461 atctgaaacg acgatccagt accggccagc tcgagcttgg caatccgcac atcggcgtca
     2521 accgcttcgc cgtaagtccg gacgtcgatc cctgcccgcc gcgcgaaacg cgcgagttgc
     2581 atcgcgcccg catcgtccgc gcacaccacc aggaagccgt ctggggcgat cctccccgcg
     2641 aagcgctcga aggcctgatg gatctcagcg agtccggcgt agtggttgag gtggtcggct
     2701 tcgatattgg tcacgatcgc ggcaaacggt gacagcaaca ggaagctctc gtcgctctcg
     2761 tccgcctcgg cgacgaacaa cccaccgacg ccatcgtgag cattggaacc cggctcgttc
     2821 aggtcggcac cgatggcgaa cgacgggtcc tcgccacagt gctgaagtgc acgcgcgagc
     2881 atcgaggtcg tcgtggtctt gccgtgcgta ccggcaactg cgattccgcg gcgaccggcc
     2941 attaccgagg caagcgcggc cgcacggggc agtatccgca acccgcgggt gcgcgcctcg
     3001 atgagttccg gattggattc ctgaatcgcc gtggacacca cgacggtgtc ggcggcaccg
     3061 aggttcgcgg cgtcatggcc gacaaacacc ttcgctccga gcacgcgaag tgccgcgagc
     3121 acccgggctt ccttcgcatc gctgccggtc accgtcgtgc cgcgtgtgag catgatgcgc
     3181 gcaatgccgg acatgccacc gccgccgatg ccgatgaaat gcacgacgcc gagctcgccg
     3241 accggcacga cgtccgaccg cagattcgcg ctcatctccc gcggccgccg cgcggccgcc
     3301 ccgctcggtc ggcgtcgctg cccgacgctt cgaggatcat gtccaccatt ttctcgtccg
     3361 cgtcgcgacg gcccaaatcg gcggcccgca ccgacatcgc gtgcagcctg cgcgggttcg
     3421 tcagcagcgg gatcacctgg tcgcggatcc acgctgggcg cagttcggcg tccgacacga
     3481 gaagtccgcc gcctgaatcg acgatcggcg cggcgttgaa gcgctgctcg ccgttgccat
     3541 gcggcaaggg cacgtagatc ccgggcaacc ccaccgcggt aagctcggcg cacgtcatcg
     3601 ctccggcacg gcacagcatc agatcggccg cggcgtacgc cagttccatc tggtcgacat
     3661 agggcacgag cacgtacggg ggctgtccgg caatcggttc gggcttcgcg gcatcgacgt
     3721 cagcgacctg agtcggaccg gcgatgtgca gcacctggat gcccgcggcc agcaagtcgg
     3781 cgacaacgcc aaccacggcc cggttgagcg agcgcgcgcc ttgcgatccc cccgacacca
     3841 gcaacgtcgg gacgtcgtcg aggaggccaa gttgagcgcg cgcgcgaccg cgcaggtcgg
     3901 cgcggtcaag tgtgctgatc gctcgtcgaa gcgggacccc gatcacctgc gcatgtggca
     3961 gcttcgggtc gggcgtgctc gccgcgacgt acggcgtgaa ccgagcgccc catcgattgg
     4021 ccagcccggg cttggcgttg gcttcatgca ccacgaaggg cagcttcgcg cccctggcag
     4081 caagataggc aggcagcgcc acgtacccgc caaacccgac gacgacgtcg gcgccaacgt
     4141 tgtcgaaaat ggcggtcgct tcacgcacgg cggtccgcat ccgggttgga accgttagga
     4201 acgacggagt gagcgagcgc ggcaacggca ccggtggtat gaacgcgagg tcatagccgc
     4261 gcgccggtac caaccgggtc tcgagcccgc gcgccgtacc gagcatggtg atgccgacgt
     4321 cggggtcgcg acgtcgcaac gcatcggcga cggcaagggc gggctcgaca tggccagcgg
     4381 tacccccgcc ggcgagcacc acatgcaccc gacttcaccg accgccgcgc gacagatgca
     4441 gtactactgc tgtcttatgc agacctcggc ggaatacgtt ggggcctcgc gcggcaatgg
     4501 cggccgcagc gctcggctct cgacgcgcga gcgacatcaa catcccgagg gcgaacatgg
     4561 t
//
LOCUS       DATCBJ010000613         6477 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_55314, whole genome shotgun sequence.
ACCESSION   DATCBJ010000613 DATCBJ010000000
VERSION     DATCBJ010000613.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 6477)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6477)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6477
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_55314"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            88..1440
                     /locus_tag="VII50_12330"
     CDS             88..1440
                     /locus_tag="VII50_12330"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis tyrosine autokinase"
                     /protein_id="HEY5051679.1"
                     /translation="MDARRFWHAVAHRWIVIAMLVVLGPLIGAVVALGSPVHYRATAA
                     VLISSDHIGSIDDLGAGSQLAVNVAPSFAALVTSPAVLSPAITALGLDSSPGQLAPAV
                     RVTVALASSTVTISLTATGRTTAAALANAVADQFALLVPKLSPTLENSPAFKATKIEP
                     AYPPASDTGLRVGAGVAIGLIAALAICWPIVAAYAANPVVDRREVAARATAVPVLGTL
                     PAAGRTATTGHRAAAGDSGSERDRSVLSTLAAVAPAVQCLMVASSRAGDGRTRTAVNM
                     AVSGVQSSRSVLLVDADLRESSVAGLLDLDGSAGLQGILAGTSTFADVVQPVGSHGLH
                     VITAGVNPRGVGNPLLASAVMSRFLATARERYDLIVIDTPPIATSSDALGLAAQVDGI
                     VVVVDARRTRQRTLTTTLRRLTLAGGPVVGIIVNRTSSPWRLPQLPDHDADRQRLAGV
                     "
     gene            1437..2756
                     /locus_tag="VII50_12335"
     CDS             1437..2756
                     /locus_tag="VII50_12335"
                     /inference="COORDINATES: protein motif:HMM:NF016800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HEY5051680.1"
                     /translation="MSAQQAVERPWHVDLDRAPRTGHLDQTVVLMVLTWLAVVLPRFV
                     QSLTAPKHEALINDAIPYSSSTSFLSHLLTLLVIGWSVLVVARGAANLPTDRRRVLVI
                     LLAPWIYLVTRDLYSGVHLHLQDLEYPAIVIAVWVLRPPIRKLALLGYLAAALALISV
                     LMGVFLKSKGLYHASNGDLVPLGKKILPWGILVGPLTSENNLGQVLVLGLPLIGLARS
                     RIVRLLVVALTTYAVVWTASRSSIAALAVGGLIALALQVPGRKLRQVVAAVLVLAAGL
                     VMIALPVSTSDGTAFSNRGYIWIASLRAWSQHRLFGLGSQWFSSLAKYEEGLGGQAFH
                     GHNTFVQMVVMGGAVLLVLVAAMAVVSLGSAVRWVGRGVNAPAVLLGTFFVSATLEWS
                     FAFYDRDFLLAVTVLPMAMVLFAERPRDDEPASGADDQTQQLDASHP"
     gene            complement(2709..3986)
                     /locus_tag="VII50_12340"
     CDS             complement(2709..3986)
                     /locus_tag="VII50_12340"
                     /inference="COORDINATES: protein motif:HMM:NF014732.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzz/FepE/Etk N-terminal domain-containing
                     protein"
                     /protein_id="HEY5051681.1"
                     /translation="MSLDSYLSILRLRWLTILAVIVVSVLGTALYSYNATPRYEAHAG
                     VFFSVSVSDNAGTRSRGFTYQQTQVRTFAQLATLPIVLNPVIKKLNLTTTADKLADEI
                     TAQAPLNTPIVDITVSGSSRDRAIAIAGAVAAQLSTTVVSLAPKVDNGTATVVATTVN
                     PASAGSSPAVPRTRLNLLVALVLGAMVGALLALALDARETRAVRRRQLEALGGAAVIG
                     TVTMPSDNGRQGGGGARWADGLQQLQLRLFPPLQTRTFTSVAITSGADPGLSARLAAG
                     LAVAQTSAPQRVLLIDANLHQPSVGASLGLPASAGLVGVLRGEVGLDAATQRVGNHGL
                     DVLAAGPGPLDPTGMVDSDQMRALLHRAGQRYDLVIVNAPPFVTADGLALAGLTDRAV
                     AVLARDETRGALADPLATLRVARVQLLGLVVST"
     gene            4211..6130
                     /locus_tag="VII50_12345"
     CDS             4211..6130
                     /locus_tag="VII50_12345"
                     /inference="COORDINATES: protein motif:HMM:NF024832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HEY5051682.1"
                     /translation="MTDETLFDAHPDRYLPVPDFRLPFTAYYANDLSHDGVSGSAHNG
                     GAHNGGAHNGSAQNDRAAAGSPLEGKSSSGDAAVLTEVFTEMLFDYGDTQSLPPLTQL
                     VDLPDPFDPSRHAPSEPTDSPELASDLVNEPMEKPAGGLGNAAARGTGITLAMQCVRF
                     VLQFCSLVVLARLLTPADFGIVAMVTAIVGVADIMRDFGLSSAAIQAKFLNNAERTNL
                     FWVNVGIGCAGALVILLCTPLISRLYGQHHLAPIIFSLAWILIVSGVNTQFNAELSRS
                     LRFKALAFADIGAQALGIAAAITVAALGGGYWAIVVQQIVVAVMTLTFNMAVCRWRPG
                     LPRRSVSITRFFRFGGGVFGTQLIAYATKNIDNVAVGAYWGAGPLGLYSRAYQLLMTP
                     LSQINAPLTRVALPVLSRVQEDDVVFARYLKKAQLVGCYLTATVFAMCAALATPLVAV
                     LFGPKWHGVAPIFALLAIGGVFRSISQISYWIFLSRGKTAQQFKQYLVTRPIMIAIIL
                     GGLPWGPKGVAVGASIAYFMDWAVSLWWVGRAAHVDSRMLFRNAFRSLFLVSAPCGVV
                     AYLGTLLVSQPIAQLAVAGVLAIVYVAVLIAVVPTVRDDAKMILYFVRRSIGRAKPKP
                     RHRAPQVQLLFTRQP"
     gene            complement(6137..>6477)
                     /locus_tag="VII50_12350"
     CDS             complement(6137..>6477)
                     /locus_tag="VII50_12350"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEY5051683.1"
                     /translation="GDGELSSFFAAADVVACPFLDVTTSGSAMLALSFGVPIVAPRIG
                     ALADLPDDVGFFYEPAEPGGLAAALEHALGSPDDVAKAAHQAHDYAAGMTWARAAEAT
                     IAVYQRVVPL"
BASE COUNT          962 a         2143 c         2176 g         1196 t
ORIGIN      
        1 gcggacgggc gcttctgggg ccagtagtgg ccagcccggc gatccacggc gcgggcccgg
       61 gtcctgactg aaacggcggg atccctcatg gacgcacgcc ggttctggca cgccgttgca
      121 caccgctgga tcgtcatcgc catgctcgtc gtgctcggac cactgatcgg ggcggttgtc
      181 gccctggggt cgccagtgca ttaccgggcc accgcggccg tgctcatctc cagtgatcac
      241 atcgggtcga tcgacgacct cggcgccggc agccagctag ccgtgaacgt cgcaccctcc
      301 tttgcggcac tcgtgacctc acccgccgtg ctgtctcctg ccatcaccgc gctggggctc
      361 gacagctcac ctggccagct cgcccctgcg gtgcgcgtca cggttgcgct ggccagcagc
      421 acggtgacga tcagcctgac cgcgaccggt cgcacgaccg ccgcagcgct ggcgaatgcg
      481 gtggctgacc agttcgcgct gctcgtgccg aaactgtcac cgacgcttga gaactcgccc
      541 gcgttcaagg cgacgaaaat cgagcctgcg tacccgccgg cttccgacac tggcctgcgg
      601 gtaggagccg gtgtagccat cggactgatc gcggcgctcg cgatctgctg gccgattgtc
      661 gccgcgtacg cggcgaaccc cgtcgtcgac cggcgcgagg ttgccgcccg cgccaccgcc
      721 gtaccggtgc tcggtacgct gcctgcggcg ggtcgcacgg ccacgacagg gcaccgggcc
      781 gccgccggcg attccgggtc ggagcgcgac cgcagcgtgc tgtccacgct cgcagcggtg
      841 gcgcctgcgg tgcaatgcct gatggtggcg tcgtcgcgcg caggcgatgg cagaacgcgc
      901 accgcggtga acatggcagt gagtggtgtg cagtcgtcgc gcagtgtctt gctcgtcgac
      961 gccgatctgc gcgagtcgag cgttgccggc ctcctcgatc ttgatggatc cgctggactg
     1021 caagggattc ttgccgggac gtcgactttc gccgacgtcg tgcagccggt tggcagccac
     1081 ggcttgcacg tcatcacggc aggcgtcaac ccacgcggag tgggcaatcc gctcctcgcg
     1141 tccgccgtca tgagccgatt cttggcgacg gcgcgagagc gctacgacct catcgtgatc
     1201 gacaccccgc cgattgccac gtcgagcgat gcccttggtc tggccgcgca ggtcgacggc
     1261 atcgtggtcg tggttgatgc gcgacgcacg cgccagcgga cgctgacaac gacgctgcgc
     1321 cggttgacgc tggctggtgg tccggttgtg ggcatcatcg tgaatcggac gtcgtcaccc
     1381 tggcggcttc cgcagttgcc ggatcacgac gcagaccgcc aacggttggc gggcgtgtga
     1441 gcgcgcaaca ggccgtcgag cggccgtggc acgtcgatct cgaccgggcg ccgcggacag
     1501 gccacctcga ccagaccgtc gtcttgatgg tgttgacctg gctcgctgtc gtgttgcctc
     1561 gcttcgtgca aagtcttacc gccccgaagc acgaggcgtt gatcaacgat gccatcccgt
     1621 attcttcgtc gacgtcgttc ctgagccatc tgctgaccct gctggtgatc ggctggtccg
     1681 tgctcgtcgt cgcccgaggg gcggccaacc tgccgaccga tcgccgccgc gtgctggtca
     1741 tcttgctggc gccgtggatc tacctcgtca cgcgcgacct gtactccggt gtccatctac
     1801 atctgcagga tcttgaatac ccggcgatcg tcattgcggt ctgggtgttg cggccaccga
     1861 tccgcaagct cgcattgctc gggtacctgg cggcagcctt ggccctgatc agcgtgctga
     1921 tgggcgtgtt cttgaagtcg aagggcctct accacgcgag caacggcgac ttggttccgc
     1981 tcggcaagaa gatcctgccg tggggaatcc tggtcggtcc gttgacctct gagaacaacc
     2041 tgggccaggt gctcgtcctc ggcctgccgc tgattggcct tgcgcgcagt cgcatcgttc
     2101 ggctgctcgt cgttgccctc acgacgtacg cggttgtgtg gaccgcgtcc cgctcgtcga
     2161 tcgcggcgtt ggcagtcggc ggtctcattg ccctcgccct ccaggtgccc ggccgcaagt
     2221 tacggcaggt cgttgcggcg gtgctggtct tggccgcggg tcttgtcatg attgcgctcc
     2281 cggtgtcgac gtccgacggc accgccttca gcaatcgcgg ctacatctgg attgccagcc
     2341 tgcgtgcgtg gtcacagcac cgcctcttcg ggctcggcag ccagtggttc tcgtcactgg
     2401 cgaaatacga ggaaggactg ggcggccagg cgttccacgg tcacaacacc ttcgtgcaga
     2461 tggtggtgat gggcggcgca gtgcttctcg tccttgtcgc cgcgatggcc gtggtctcgc
     2521 tggggtctgc ggtccgctgg gtcggtcgcg gcgtgaacgc gcccgcggtg ctgctcggca
     2581 cgttcttcgt gtcggcgacg ctcgaatggt cgttcgcgtt ctacgaccgc gacttcctgc
     2641 tcgccgtcac cgtgctgccg atggcgatgg tcctgttcgc cgagcggccg cgcgacgacg
     2701 agcctgcgtc aggtgctgac gaccagaccc aacagctgga cgcgagccac ccgtagcgtc
     2761 gcgagcgggt ctgcaagggc gccgcgcgtc tcatctcgtg cgagcaccgc aacggcgcgg
     2821 tcagtcaggc ccgccagcgc caatccgtcg gcggtgacaa acggcggggc attcacgatc
     2881 accaggtcgt agcgctgtcc ggcccgatga agcagcgccc gcatctggtc ggagtcgacc
     2941 atgccggtcg gatcaagggg tccgggcccg gcggccagca cgtcgagtcc atggttgccg
     3001 acacgttggg tggccgcgtc aaggcccacc tcgccgcgca gcaccccgac gagtccggca
     3061 gacgcgggga ggccaagtga cgcgcccacc gatggctggt gcaggttggc gtcgatcagc
     3121 aacacccgct gcggcgcgct ggtttgcgca acagcgaggc cggccgccaa tcgcgccgac
     3181 agtccggggt cggcgcccga ggtgatcgcc accgaagtga aggtgcgcgt ctgcaatggc
     3241 gggaagagcc gcagctgcaa ctgctgcaag ccgtcggccc atcgcgcgcc gccgccgccc
     3301 tggcgcccgt tgtcgctagg catcgtgact gtgccgatca ccgcggcgcc gccgagcgct
     3361 tcgagttgac gtcgccgcac ggcgcgagtc tcacgagcgt cgagggcgag cgcaagcagc
     3421 gcaccgacca tggctccaag caccagtgcc acgagcaggt tgagcctggt acgcgggaca
     3481 gcgggagagg accccgcgct cgcagggttg acggtcgtcg ccacgacggt cgcggtgcca
     3541 ttgtcgacct tgggggccaa gctgacgacg gttgtcgaca gctgggccgc caccgcaccg
     3601 gcgatggcga tggcgcggtc gcgcgacgac ccggacacgg tgatgtcgac gatcggtgtg
     3661 ttcagcggcg cctgcgcggt gatctcatcg gccagcttgt cggccgtcgt ggtgaggttc
     3721 agtttcttga tcaccgggtt cagcacgatc ggcagcgtcg cgagctgcgc gaacgtgcgc
     3781 acctgggttt gctggtaggt gaagcctctg ctgcgcgtgc cggcgttgtc actgacgctg
     3841 accgagaaga acacgccagc gtgtgcctcg tagcggggcg tggcgttgta ggaatacagc
     3901 gccgtgccaa gcacggagac gacgatgacg gcaaggatcg tcagccatcg caagcgaagg
     3961 attgacaggt agctgtcgag actcacgaca tcaatcgtag atcaactcac ttgcatacag
     4021 ggcaagaacc ggccctgtcc ctcaggccaa acgggtggga tttagcgccg aacagcgggc
     4081 cagccgacgc cagctgggcg gctggccaat cctggcaaag cagccgttcg gccacccgcg
     4141 ctccggtaag ctggcgaggt tcggttgagc gcgcgagcgc gcgagtgtgg tggcatggcg
     4201 agggggctcg gtgacggacg agaccttatt tgacgctcac cccgaccgct atctgccggt
     4261 gccagacttc cgtcttcctt tcacggcgta ctacgccaac gacctgtccc atgacggtgt
     4321 gtcaggcagt gctcacaacg gcggtgctca caacggcggt gctcacaacg gcagtgctca
     4381 aaacgacaga gccgccgcgg ggtcgccgct cgagggcaaa tcgtcgtcag gtgatgccgc
     4441 ggtgctcacc gaggtgttca ccgagatgct cttcgactac ggcgacacgc aatccctccc
     4501 gccgctgacc caactcgttg acctccctga tccctttgac ccctcgcgcc acgcgccgtc
     4561 ggagccaacg gactcgcccg aactggcaag cgatctcgtc aacgagccga tggagaagcc
     4621 ggctggcggt ctgggtaacg cggccgcccg tggtactggc atcactctgg ccatgcaatg
     4681 cgtgcgcttt gtgctgcagt tctgctctct tgtcgtgctg gctcggctac tgacaccggc
     4741 ggacttcggc atcgtcgcga tggtgaccgc gatcgtcggg gtggccgaca tcatgcgcga
     4801 cttcggtttg tcatcggccg cgattcaggc gaagttcctg aacaacgcgg agcggaccaa
     4861 cctcttctgg gtcaacgtcg gcattggctg cgccggcgcg ctcgtaatct tgctctgcac
     4921 gccgctgatc agtcgcctct acggccagca ccatttggca ccgatcatct tctctcttgc
     4981 gtggattctc atcgtcagtg gcgtcaacac gcagttcaac gcggagctca gccgcagtct
     5041 gcggttcaag gcgctcgcat ttgccgacat cggtgcgcag gcgctcggca tcgccgcggc
     5101 gatcacggtg gccgcgctcg gcggtgggta ctgggcgatc gtcgtgcagc agatcgtcgt
     5161 cgccgtcatg acgctgacct tcaacatggc cgtctgtcgt tggcggcctg gcctgcccag
     5221 gcgttcggtg tccatcacca ggttcttccg ctttggtggc ggagtgttcg gcacgcagtt
     5281 gatcgcctac gcaaccaaga acatcgacaa cgtcgccgtt ggtgcctact ggggcgccgg
     5341 cccgctcggt ctctacagcc gggcttacca gttactgatg acaccgctct cacagatcaa
     5401 cgcgccgctc acccgcgtgg cgttgccggt gctgtcacgt gtgcaggaag acgacgttgt
     5461 cttcgcgcgg tacctgaaga aggcacaact cgttggctgt tacctgacgg ccaccgtgtt
     5521 tgcgatgtgc gcggcgctgg cgactccgct ggtcgcggtg ctgttcgggc cgaagtggca
     5581 cggcgtcgca ccgatcttcg cgctgctggc gatcggcggc gtgttccggt cgatctcgca
     5641 gatctcctac tggatcttct tgtcgcgtgg caagaccgcc caacagttca agcagtatct
     5701 cgtcacgcgg ccgatcatga tcgccatcat cttgggcggt ctgccgtggg ggccaaaggg
     5761 cgttgcggtc ggggcctcga tcgcgtactt catggactgg gcggtctcgc tgtggtgggt
     5821 cggccgggcc gcgcacgtcg acagtcggat gctgttccgc aatgccttcc ggtcattgtt
     5881 cctcgtcagc gcgccgtgtg gcgttgtcgc gtacctcggc accctgctgg tcagccagcc
     5941 catcgcgcag ctcgcggtcg ccggcgtcct cgccatcgtg tacgtggcgg tgctgatcgc
     6001 cgtggtcccg accgttcgtg acgacgccaa gatgatcctg tacttcgtgc ggcgctcgat
     6061 cggtcgcgcg aagccgaagc cacgtcatcg cgcgccgcag gtgcagttgc tattcacccg
     6121 tcagccgtag aggtcgttac agcggcacga cgcgctgata cacggcaata gtcgcttcgg
     6181 cagcgcgtgc ccaggtcatg ccggccgcgt agtcgtgagc ttgatgggct gctttcgcga
     6241 catcgtcggg cgagccgagc gcgtgttcga gagcagctgc gagaccgccc ggctccgcgg
     6301 gctcgtaaaa gaagccgacg tcgtcgggaa ggtcggcgag ggcaccgatg cgcggcgcca
     6361 cgatcggtac gccgaacgac agcgccagca tcgctgatcc gctcgttgtg acgtcgagga
     6421 aagggcacgc gacgacgtcg gccgccgcaa agaagctgct gagttcgccg tcaccga
//
LOCUS       DATCBJ010000614         2830 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_262252, whole genome shotgun sequence.
ACCESSION   DATCBJ010000614 DATCBJ010000000
VERSION     DATCBJ010000614.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2830)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2830)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2830
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_262252"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..723)
                     /locus_tag="VII50_12355"
     CDS             complement(<1..723)
                     /locus_tag="VII50_12355"
                     /inference="COORDINATES: protein motif:HMM:TIGR03083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maleylpyruvate isomerase family
                     mycothiol-dependent enzyme"
                     /protein_id="HEY5051684.1"
                     /translation="MTTAVDVCTIPRIMHAEAMRIADVEADRVAATLRNLEPGDWAKP
                     TDCPRWDVRALAAHMVGSAASQASPREFLRQVRAGRPVVAEIGGRYWWDGMNEIQVRE
                     RSDLRTEQLIAEWDHIAPRALKARRKLPRPIAKLPLLKLPAPVGRQPLAYLFNVGFTR
                     DTWMHRIDLAEATRKPLNPDADHDGRIVADLVAEWATTHHLPFVLELTGPAGGHYTQG
                     TAGEHITIDAIEFARTLAERAHG"
     gene            complement(720..1340)
                     /locus_tag="VII50_12360"
     CDS             complement(720..1340)
                     /locus_tag="VII50_12360"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEY5051685.1"
                     /translation="MTAQPDRRKVRHQAKRAEILAQAWLLAHRDGLAAISLRDLADSV
                     GLRQPSLYVYFESKLNLYDAMFADGYRQLLSFLADRSYSGDPRRALARFLGDLVQFSS
                     DDIVRHQILFQRTIPGFEPSAEAFALALEFYEVSRQLIAAAGVTDQRNIDIFSALANG
                     LAHQQVANEPGGQRWVRLASRVTEMFLTDIDRRRTTPPLKKGPRRS"
     gene            complement(1366..1674)
                     /locus_tag="VII50_12365"
     CDS             complement(1366..1674)
                     /locus_tag="VII50_12365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5051686.1"
                     /translation="MAAVITQTWAGFTPAQYDAVRDIVGWDRHTPDGMRLHVATFDSG
                     VLNMTDVWDSEAQFMTFVQTQIMPAVTQLGIAGQPEMAVSSLYELNNITFAQTPPAKG
                     "
     gene            1951..>2830
                     /locus_tag="VII50_12370"
     CDS             1951..>2830
                     /locus_tag="VII50_12370"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HEY5051687.1"
                     /translation="MAAPKIPGVEELLALAVAGIGGEPRDGQLTMARAVEHAMLSGDH
                     LLVQAGTGTGKSLAYLVPAIRHALAMDAPVRKPKTDNEADDGGRGSDDELPPPRGPVV
                     VATATIALQRQLIERDLPRLAESLAPALPVAPTFAILKGRRNYLCLQRLHAGPDAEQD
                     GLFDDVGPSGPAGSGATGPTSALGRDVARISEWANTTDTGDRDELLPGVSERAWSQVA
                     VSSRECLGATNCPYGADCFAESARMRAMRADVVVTNHALLAIDALSEVDVLPEHDVVV
                     IDEAHELVDRVTGVATE"
BASE COUNT          436 a          870 c         1003 g          521 t
ORIGIN      
        1 gccgtgggcg cgttcggcca gggtacgggc gaactcgatc gcatcgatgg tgatgtgctc
       61 gccggcggtg ccttgggtgt agtggccgcc ggccggtccg gtcagctcga gcacgaatgg
      121 caggtggtgg gtggttgccc attcggcgac caggtcggcg acgatgcggc cgtcgtgatc
      181 ggcgtcgggg ttgaggggtt tgcgagtggc ctcggcgagg tcgatgcggt gcatccacgt
      241 atcgcgggtg aacccgacgt tgaacaggta ggcgaggggt tgtcgtccga caggggcagg
      301 gagtttgagt agtggcagct tcgcaatggg gcggggcagc ttgcggcggg ccttcaacgc
      361 gcgcggtgcg atgtggtccc attcggcgat gagctgttcg gttctgaggt cgcttcgttc
      421 acggacctga atctcgttca ttccatccca ccagtaccgc ccgcctattt cggcgacgac
      481 ggggcgaccg gcccggactt ggcggaggaa ctcgcgtggc gaggcctggc tggcggcgga
      541 cccgaccatg tgcgcggcca gcgcgcgcac atcccaccga gggcagtcgg tgggtttggc
      601 ccagtcaccc ggctccaggt tgcgtagcgt cgcggcgacc cggtcggcct cgacgtcagc
      661 gatccgcatt gcctcggcgt gcatgatccg cgggatcgtg cacacgtcaa ctgcggtcgt
      721 catgatcgtc ggggtccctt cttaagtggt ggcgtggtgc gacgtcgatc gatgtcggtg
      781 aggaacattt cggtgacacg actggccaac cggacccagc gctggccacc tggttcgttg
      841 gctacctgtt ggtgggccaa cccgttggcc agggcactga atatgtcgat gttgcgttgg
      901 tcagtgacgc ctgcggcagc gatcagctgg cggctgacct cgtagaactc cagcgccaag
      961 gcgaaggcct cagccgatgg ctcaaagccc gggatggtgc gctggaacag gatttggtgc
     1021 cgcacgatgt cgtcgctgga gaactgcacg aggtcgccga ggaatcgtgc caaggcacgg
     1081 cgcggatcac cgctgtagct gcggtccgca aggaaactca gaagttggcg gtagccgtcg
     1141 gcgaacatcg cgtcgtaaag gttcagcttc gattcgaagt acacatacag cgagggttgg
     1201 cgaaggccca cactgtcggc caggtctcgc agcgagatcg ctgcgaggcc gtcgcggtgc
     1261 gcgaggagcc aagcctgagc aagaatctcg gcacgcttcg cctggtggcg gacttttctg
     1321 cggtctggtt gagccgtcat catctcgcag cccggcagag cggtgtcatc ccttggcggg
     1381 tggggtctgc gcgaaggtga tgttattgag ctcgtacagt gaactgacgg ccatctccgg
     1441 ttggccggcg atacctagct gcgtgacggc cggcatgatc tgcgtctgca cgaacgtcat
     1501 gaactgcgcc tcggagtccc acacatcggt catgttgagt accccgctgt cgaaggtggc
     1561 cacgtgtaag cgcatcccgt ccggcgtatg ccgatcccag cccacgatgt cgcggaccgc
     1621 gtcgtactgg gcgggagtaa agccggccca tgtctgtgtg atcactgcgg ccacgatgtg
     1681 cctcctaggt tgattcggcc gatggtttcg caccggtcgt gcgtggttgt cggtcggggt
     1741 tggctcggca tcgtagccga cagcccgtag tttcccgatc gacgatagga agcctatcgg
     1801 tcataggctg acagtcaagg gtgtcgtcgt gtatggcgtc gccgagttgc gtcgttcgcg
     1861 aaccaggcgc gtcgcggcgg cacggccgga cctagccggg ctgtgggtgc gcgccgaatc
     1921 gacagccgcc ggccagctac ccttcgagcc gtggctgcac cgaagattcc tggcgtcgag
     1981 gaactgctcg ccctcgccgt tgccggcatc gggggcgagc cgcgcgacgg ccagctgacc
     2041 atggcccgcg cggtcgagca cgcgatgctc tcgggtgatc atctgctcgt tcaggcgggc
     2101 accggcaccg gcaagagcct cgcctacctg gtccccgcga ttcgccacgc gctcgccatg
     2161 gatgcgccgg ttcgcaagcc caaaaccgac aacgaagcgg acgacggggg gcgcggctca
     2221 gacgacgagt tgccgcctcc ccgcgggccc gttgtggtgg ccaccgccac tatcgcgttg
     2281 cagcggcagc tgatcgagcg cgatctgccg cggcttgccg aatcgctcgc gcccgcattg
     2341 ccagtcgcac cgaccttcgc gatcctcaag ggacgccgga attacctgtg cctgcaacgc
     2401 cttcatgctg gtccggacgc tgagcaggac ggtctcttcg acgacgtagg gccttcgggc
     2461 ccggccgggt cgggcgctac cgggccgact tcggcgctcg gtcgcgacgt cgcgcgaatc
     2521 agcgaatggg cgaacaccac cgacaccggt gaccgcgacg agctgctccc gggtgtcagc
     2581 gagcgggcgt ggagtcaggt cgcggtctcg tctcgtgagt gtctcggcgc gaccaattgt
     2641 ccttacggcg cagactgttt cgccgagtct gcgcgcatgc gggcgatgcg cgccgacgtc
     2701 gtcgtcacca accacgcgtt gctcgcgatc gatgcgttga gcgaggtcga cgtgctgccg
     2761 gagcacgacg tcgttgtcat cgacgaggca cacgagctgg tcgaccgggt cactggcgtc
     2821 gcgaccgagg
//
LOCUS       DATCBJ010000615         3525 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_219245, whole genome shotgun sequence.
ACCESSION   DATCBJ010000615 DATCBJ010000000
VERSION     DATCBJ010000615.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 3525)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3525)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3525
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_219245"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..198
                     /locus_tag="VII50_12375"
     CDS             <1..198
                     /locus_tag="VII50_12375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5051688.1"
                     /translation="ASRAGVSPDAYAQQLVSSGTVPLAVADVLRGKALAYVLENAVVT
                     DASGRIVDLNRLEQDVAAGSS"
     gene            423..1013
                     /locus_tag="VII50_12380"
     CDS             423..1013
                     /locus_tag="VII50_12380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HEY5051689.1"
                     /translation="MPSSGVSPDDDVFNKLLKNRIVILGSVVDDAIANKICAQLLLLA
                     AEDPEREIYMHINSPGGSVDAGMAIYDTMQYVPCDVATVGMGLAASMGQFLLCAGERG
                     KRYALPHARIMMHQPLGGIGGSASLIAIQAEQMLYTKKKLQELTAQHTGQTVDQIERD
                     SDRERWFSADEAKDYGFVDHVVRSARQVPTETTAVS"
     gene            1031..1726
                     /locus_tag="VII50_12385"
     CDS             1031..1726
                     /locus_tag="VII50_12385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HEY5051690.1"
                     /translation="MNSNVPPDLFDVADAGASGLARPTGLQRPDGRYIIPEFRERTST
                     GDRIHNPYTKLFEERIIFLGVQIDDVSANDVMAQLLTLESMDPDRDIQLYINSPGGSF
                     TALTAIYDTMQFIRPEVQTICMGQAASAAAVILAAGAKGKRIALPNSRILIHQPSSEA
                     GGQASDIEIAAREILRMRALLEFMLAKHTGRTEAQVRIDIERDKILTAEEAVEYGLID
                     EVIVSRKLTLAAH"
     gene            1882..3171
                     /gene="clpX"
                     /locus_tag="VII50_12390"
     CDS             1882..3171
                     /gene="clpX"
                     /locus_tag="VII50_12390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014816688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HEY5051691.1"
                     /translation="MARIGDGGDLLKCSFCGKSQKQVKKLIAGPGVYICDECIDLCNE
                     IIEEELSESADLKWDELPKPREIYDFLEQYVIGQDIAKKALSVAVYNHYKRIQASGKE
                     SRTGSDTVELAKSNILLIGPTGCGKTYLAQTLARMLNVPFAIADATALTEAGYVGEDV
                     ENILLKLIQAADYDVKKAETGIIYIDEIDKIARKSENPSITRDVSGEGVQQALLKILE
                     GTTASVPPQGGRKHPHQEFIQIDTTNVLFIVGGAFSGLEKIIESRVGKKGIGFAATLH
                     TRRDADAVDAFADVMPEDLLKFGMIPEFIGRLPVITTVANLDREALIRILTEPKNALV
                     RQYKRLFDLDSVELEFNEEALEAIADQAILRGTGARGLRAIMEEVLLSVMYDIPSRKD
                     VARVLINREVVLDKVIPTLVPREAAASVKRERREKTA"
     gene            complement(3211..>3525)
                     /locus_tag="VII50_12395"
     CDS             complement(3211..>3525)
                     /locus_tag="VII50_12395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine phenol-lyase"
                     /protein_id="HEY5051692.1"
                     /translation="FLPHIPPLQYPGQSLAIALYETGGVRGCEIGTVMFGRQPDGTEL
                     PASLDLVRLAIPRRTYTQSHIDYTIEVCEAVATQAADLRGMRIVDEPPSLRHFTATFE
                     PL"
BASE COUNT          671 a         1160 c         1123 g          571 t
ORIGIN      
        1 gcctcgcgcg ccggagtgag tccggacgcc tacgcgcagc agctggtgag cagcgggacg
       61 gttccgctcg ccgtcgccga cgtgttgcgg ggaaaggcgc tcgcctacgt gctcgagaat
      121 gcggtggtca ccgacgcgtc cggccggatc gtcgacctca accgccttga gcaggacgtc
      181 gccgccgggt cttcgtgacc gaggttgcgt cgcggctgcc ggggtgcgcc tacagcgaac
      241 agcgcgcggg agtgtcccgc ggaacacgat cggccggtta gggtcggatc gttggcaccc
      301 gataactcca gtaggcacgt cgagcgcgcg ccgtagcgcg gccgaccagc gaagacgagc
      361 aggtgaagag cgtggcgctg tactccccgg cctccggttc ggctgacgat ccgcaggggc
      421 ggatgccgtc gtccggcgtg tcccccgacg acgacgtttt caacaagctg ctcaagaacc
      481 gcattgtcat tctcgggtcg gtggtcgatg atgcgatcgc caacaagatc tgtgcgcagc
      541 tgttgttgct cgccgccgaa gaccccgagc gcgagatcta catgcacatc aactcccccg
      601 gtggctcggt cgacgccggc atggcgatct acgacaccat gcaatacgtc ccgtgcgacg
      661 ttgcgacggt cggcatgggg cttgcggcgt cgatgggcca gttcctgctc tgcgccggcg
      721 agcgcggcaa gcgctacgcg ctcccgcatg cgcggatcat gatgcaccag ccgctcggcg
      781 gcatcggcgg ctcggcgtcc ctcatcgcta tccaggccga gcagatgctc tacacgaaga
      841 agaagcttca ggagctcacc gcgcagcaca ccggccagac cgtcgaccag atcgagcgag
      901 actccgaccg cgaacggtgg ttctccgcag acgaggcgaa ggactacggc ttcgtcgacc
      961 acgtggtgcg cagcgcccgc caggttccta ccgagaccac cgcggtctcc tgaagagcga
     1021 ggacctttcc gtgaattcga atgtgccacc cgacctgttc gacgtcgccg acgccggcgc
     1081 gagcggcctc gcccgcccca ctggtcttca gcgccccgac ggccgctaca tcattccgga
     1141 gttccgcgag cgcaccagca cgggcgaccg gatccacaac ccgtacacga agctgttcga
     1201 agagcggatc atcttcctgg gcgtccagat cgacgacgtg tcggcgaacg atgtcatggc
     1261 tcagctgctg acacttgaat cgatggatcc cgaccgcgac atccagctct acatcaactc
     1321 accgggcggt tccttcaccg cgctcacggc gatctacgac acgatgcagt tcatccggcc
     1381 cgaggtacag acgatctgca tgggccaggc cgcctcggcg gccgcggtca tcctggcagc
     1441 cggagccaag ggcaagcgga tcgcgctgcc gaactcgcgg atcttgatcc accagccgtc
     1501 cagcgaggcc ggagggcaag ccagcgacat cgagatcgcg gctcgcgaga tcctgcggat
     1561 gcgggcgctg ctggagttca tgttggccaa gcacaccggt cgtaccgagg cccaggtccg
     1621 gatcgatatc gagcgcgaca agatcctcac cgccgaggaa gccgtcgagt acggcttgat
     1681 cgacgaagtc atcgtcagcc gcaagctcac cctcgcggcg cattaggccc ccggcagcca
     1741 gccggacacg ccgtaaccgg cccgaagccg gcgctgaagc ggtaccttcg gagtaccgct
     1801 gcaccaccga gtacgagcag ctagtccgat ttgtaccgca gtccgacacg tagcaggtcc
     1861 gacggtcagg agacgcatcc ggtggcacgc ataggtgacg gcggcgacct gctcaagtgc
     1921 tctttttgcg gaaagagcca gaagcaggtc aagaagctca tcgcgggccc gggcgtctac
     1981 atctgcgacg agtgcatcga cctgtgcaac gagatcatcg aggaagagct ttcggagtcc
     2041 gccgacctga agtgggacga gctaccgaag ccgcgagaga tctacgactt cctcgagcaa
     2101 tacgtcattg gtcaagacat tgccaagaag gctttgtcgg tcgcggtcta caatcactac
     2161 aagcgtatcc aggcctccgg caaagagtcg cgcaccggta gcgacactgt cgagctcgcc
     2221 aagtcgaaca tcttgctgat cggacccacc ggctgcggca agacgtacct tgcgcagaca
     2281 ctcgcgcgga tgctgaacgt gccgttcgcc atcgccgacg ccacggcact gaccgaagcc
     2341 ggctacgtcg gcgaggacgt cgagaacatc ctgctgaagc tcatccaggc cgctgactac
     2401 gacgtcaaga aggccgagac cggcatcatc tacatcgacg agatcgacaa gatcgcccgg
     2461 aagagcgaga acccctcgat cacccgtgac gtctccggcg agggtgttca gcaggctctg
     2521 ttgaagatcc ttgagggcac caccgcatcg gtaccgccgc aaggcggccg caagcacccg
     2581 catcaggaat tcatccagat cgacacgact aacgtcttgt tcatcgtcgg cggcgcgttc
     2641 tccggcctcg agaagatcat cgaatcccgc gtcggcaaga aggggatcgg tttcgccgcc
     2701 accttgcaca ccaggcgcga tgccgacgcc gtcgatgcat tcgccgatgt gatgccagaa
     2761 gacctgttga agttcggcat gatccccgag ttcatcgggc ggctgccggt catcacgacc
     2821 gtcgcgaacc tcgaccgcga ggcgctgatc cggatcctca ccgagccgaa gaacgcactc
     2881 gtgcgccagt acaagcggct cttcgatctc gactcggtcg agctcgagtt caacgaggag
     2941 gcgctcgaag cgatcgcgga tcaggcgatc ctgcgcggca ccggcgcgcg tggcctgcgc
     3001 gccatcatgg aagaagtctt gctgtcggtg atgtacgaca tcccgagtcg caaggacgtc
     3061 gcccgcgtgc tgatcaaccg cgaggtcgtg ctcgacaagg tcatcccgac gctggtgccg
     3121 cgtgaggccg cggcttcggt caagcgcgaa cggcgtgaaa agactgcctg attttgcgcg
     3181 ctgcgcgacg tgatcgcctg actgttccgg ctagaggggc tcgaaggtcg cggtgaaatg
     3241 ccgcaacgac ggcggctcgt cgacgatccg cattccccgc aagtcggccg cctgtgttgc
     3301 taccgcctcg cagacctcga tcgtgtagtc gatgtggctt tgcgtgtagg tccgccgcgg
     3361 gatcgccagg cgcaccaggt cgagggacgc cggcagttcc gtgccgtcgg gctggcggcc
     3421 gaacatgacg gtgccgatct cgcacccgcg cacaccgccg gtctcgtaca gcgcgatcgc
     3481 cagagactga cctgggtact gcagcggcgg gatgtgaggc aggaa
//
LOCUS       DATCBJ010000616         3885 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_386141, whole genome shotgun sequence.
ACCESSION   DATCBJ010000616 DATCBJ010000000
VERSION     DATCBJ010000616.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 3885)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3885)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3885
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_386141"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..666
                     /locus_tag="VII50_12400"
     CDS             <1..666
                     /locus_tag="VII50_12400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5051693.1"
                     /translation="GASSPLSGESSLQEFALFASATVTVLPSIETGLPLLFVKVNVNW
                     TAGCPDTPPWPVPDEVMSWVPLTATVKAPTVGVAVDGTGVGLLVAVGVGTGVSLAGAE
                     GDFDADGFGLALAVAFGVVPVTGVAAVAVGWAGVLADPDATAGTEAAGAEPATPVDDT
                     AGLGSVLDAAAATGPDDAALLLCDELEHADSASAATTVTPTHPVTRKRTVMAFPPAGM
                     RQQ"
     gene            944..>3885
                     /locus_tag="VII50_12405"
     CDS             944..>3885
                     /locus_tag="VII50_12405"
                     /inference="COORDINATES: protein motif:HMM:NF024777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LamG domain-containing protein"
                     /protein_id="HEY5051694.1"
                     /translation="MHAFSRRGGRSVSAVLAIVMVGSWLAVASSDAARADTAPPSPTT
                     PVTVSADALPTVQIDGVVWQQAIVGNTVYAGGSFANARPAGSAAGTNLTPRNNFLSYD
                     ITSGALNTAFVPNLNAQVKAVVASPDGTRVYVGGDFTTANGSNRWRLAAYNTATGALI
                     NTFIPNLNGGVRAIVATNTAVYVGGAFTVANGVTRNKLAAFSATDGSLLSWNPNANDG
                     INAMVMAPDGSKIIVGGQFTTLNGTAEYGMGALDPITGANLPWAADATVKDAGVNAAI
                     DTLSTDGTNIYGGGYVFGAGGNLEGVFAADPSTGAIRWIEDCHGDTYSTYSDGNAMYE
                     VGHQHYCDDVPNAFPQTNPWTIHHSQAFSTAVAGTLLHNTQGAYADFGGTPAPEQLDW
                     YPTLTTGTYTGQTQAAWTVTGNGQYVVEGGEFPTVNGVAQQGLVRFAVRAIAPNKMGP
                     QVTGSNFVPTITSITAGTARVAWQANWDQDNTALTYKVVRNSNTASPVFTTTVNSTFF
                     NRPMLGFTDTGLVVGQSYKYRLYVTDPLGNQVAGDTVTFTPTANTVSPYAQTIINDGP
                     NTYWRLGEASGTMAYDWAGYSDGTVGAGATRGAAGAIIGDSNTATTFNGTATSIVSHP
                     YSQTAPNSFSVEGWFQTTSTSGGRIIGFGDTQTGTSGDADRAVYMTNGGQLSFGVISG
                     STVTGTKTTITSSAAYNDGQWHYVVASLGSGGMSLYVDSKRVAQNAAVTTAVDYAGWW
                     RLGGDSLSGFPSQPSNVNFRGSIDDVAIYSAPLTAAQIANHYNASGRTAAGARPADTY
                     GSTIYDASPNFYWRLDETSGSVAHDITPNGNNGNYSGGVTFGTAGAITGTTDTAVTMN
                     GSNAVVSTTSAVPGPSVYSEELWFKTTTTTGGKLIGFGSSATGNSGSYDRHVYMLNNG
                     KLTFGTYTGVTNTIASSASYNDGKWHYMVATQASDGMKLYVDGALVASGSVTGAQSYN
                     GYWRVGGD"
BASE COUNT          744 a         1296 c         1218 g          627 t
ORIGIN      
        1 ggcgcgtcgt cgccgttgtc cggggagtcc tcactccagg aattcgcatt attcgcgtcg
       61 gccaccgtca cggtgttgcc gtcgatcgaa accggactgc cgctcttgtt cgtgaaggtc
      121 aacgtgaact ggacggcggg ctgtcccgac acgccgccct ggccggtgcc cgacgaggtg
      181 atgtcatggg tgccgctgac cgccaccgtg aaagcgccaa cggtcggcgt cgcggttgac
      241 ggcacgggcg tcgggctgct cgtcgccgtc ggcgtcggta ctggtgtgag cttggcaggc
      301 gcggagggcg acttcgatgc cgacggcttc gggctggcct tggcagtggc cttcggcgtc
      361 gtcccggtca caggagtcgc ggcggtcgcg gtgggctggg ctggtgtgct ggctgacccg
      421 gacgcaaccg cgggcaccga ggctgcaggt gccgaaccgg cgacccctgt tgacgacact
      481 gctgggctcg gcagcgtact cgacgcggcg gcggccaccg gtccagacga tgcggcgctc
      541 ttgttgtgtg acgagctgga gcatgcggac agcgcgagtg cggctacgac cgtcacaccc
      601 acccatccag tcacacgcaa acggacggtc atggcgtttc cccctgcagg catgcgccaa
      661 caatagacga cgcgatgttg cgaggagtgc gttcgcagaa cttcgccacg ttccacccga
      721 tgtgtctagt gaattggtag ccaggtgcgg attattgagc catttggcag ctgctcgata
      781 ggccgaacgg ataacgaatc tgtgagcaag ctgagacttg agccccgaac gggtgatgtt
      841 gcttgcgacc tcactgatac gcctgcagac atcccctcgc tcacttaacg ttgggaacgt
      901 ttacgacggc ggtgggtcgt cggtgttggg gggaaataca agaatgcacg ccttttcgcg
      961 ccgcggcggc cgctccgttt ctgcggtact tgccatcgtg atggtcggat cgtggctcgc
     1021 tgttgcgagc tcggacgcgg cgcgtgctga caccgcgccg cccagcccga ccacacccgt
     1081 caccgtgtcg gccgacgcac ttccgacggt gcaaatcgac ggcgtcgtct ggcagcaggc
     1141 gatcgtcgga aacaccgtgt acgcgggtgg cagcttcgca aacgcccgtc cggctggttc
     1201 agccgctggc acgaacctca cgccacgcaa caacttcctg tcctacgaca tcaccagcgg
     1261 cgcgctgaac accgcgttcg tgccgaacct gaacgcccag gtcaaagcgg tcgtcgcatc
     1321 acctgacggc actcgggtct atgtcggcgg cgacttcacc accgcgaacg gatcgaaccg
     1381 ctggcgcttg gccgcctaca acaccgcaac cggcgccctg atcaacacgt tcattcccaa
     1441 cctgaacggc ggcgtgcgcg caattgtcgc cacgaacacc gcggtttacg tcggaggcgc
     1501 gttcaccgtc gcgaacggcg tcacgcgtaa caagctcgcc gcattcagcg cgaccgacgg
     1561 ttcgctgctg agctggaacc cgaacgctaa cgacggcatc aacgccatgg tgatggctcc
     1621 ggacggttcc aagatcatcg tcggcggcca gttcacgacc ctgaacggca cggccgagta
     1681 cggcatgggt gcactcgacc cgatcaccgg cgcgaacctg ccgtgggcag ccgatgcgac
     1741 ggtgaaggac gccggcgtca acgcagccat cgacacactc tcgaccgacg gaaccaacat
     1801 ctacggcggc ggctacgtct tcggcgccgg cggcaacctt gagggcgtct ttgccgccga
     1861 tccgtcgacc ggagccatcc gttggatcga ggactgccac ggcgacactt actccacgta
     1921 ctccgacggc aacgccatgt acgaagtcgg ccaccagcac tactgcgacg acgtaccgaa
     1981 cgcattcccg cagacgaacc catggacgat ccaccactca caggcgttct ccacggccgt
     2041 cgcgggcacg ctgttgcaca acacccaggg tgcatacgcc gacttcggcg ggaccccggc
     2101 gcccgagcag ctcgactggt acccgacgct cacgacaggc acgtacacgg gccagacgca
     2161 ggccgcctgg accgtcacgg gcaacgggca atacgtcgtt gagggcggcg aattcccgac
     2221 ggtcaacggc gttgcgcagc agggcttggt gcggttcgcc gttcgggcga tcgctccgaa
     2281 caagatgggc ccgcaggtca ctggaagcaa cttcgttccg acgatcacct ccatcaccgc
     2341 gggcacggca cgtgttgcat ggcaggccaa ctgggaccag gacaacactg cgctgaccta
     2401 caaagtcgtg cggaacagca acactgcatc gccggtcttt accaccacgg tgaactcgac
     2461 cttcttcaac cgtccgatgc tcggtttcac cgataccggg ctggtggtcg ggcagagcta
     2521 caaataccgg ctgtacgtca ctgacccact cggcaaccag gtcgccggcg acacagtgac
     2581 gttcactcca accgcgaaca ccgtgagccc ctacgcgcag accatcatca atgacgggcc
     2641 taacacgtac tggcgcctcg gtgaggcctc cggaaccatg gcctacgact gggctggcta
     2701 ctccgacggc accgtcggtg ccggggcaac gcgcggtgca gcgggcgcga tcattggcga
     2761 cagcaacact gctacgacgt tcaatggcac ggcaacgtcc atcgtctcgc acccatacag
     2821 ccagactgcg ccgaactctt tctcggtgga aggctggttc cagacgacga gcaccagcgg
     2881 cggccggatc atcggcttcg gcgacacgca gacaggcacc agcggtgatg ccgaccgcgc
     2941 tgtctacatg accaacggtg gacagctctc gttcggcgtc atctccggca gcaccgtgac
     3001 cggcacgaag accacgatca ccagcagcgc ggcctacaac gacgggcagt ggcactacgt
     3061 tgtcgcgagc cttggaagcg gcggcatgtc gctgtacgtc gattctaagc gggtggccca
     3121 aaacgctgca gtgacaaccg cggtcgatta cgcgggttgg tggcgcctgg gtggtgacag
     3181 cctgagcggc ttcccgagcc agccgtcgaa cgtcaacttc aggggctcga tcgacgatgt
     3241 cgcaatctac tcagctccgc tcacggcagc gcagatcgcg aaccactaca acgccagcgg
     3301 tcggacggcg gccggcgcac ggccggctga cacgtatggc tcgaccatct acgacgcgtc
     3361 gccgaacttc tactggcggc tcgacgaaac gtcgggttcg gtcgcgcacg acatcacgcc
     3421 caacggcaac aacggcaact actcaggcgg cgtcacgttc ggcaccgcag gcgcgatcac
     3481 cgggacgacc gataccgcag tcaccatgaa cggttccaac gcagtggtgt cgacaacctc
     3541 ggcggtaccc ggcccgagcg tctacagcga agagttgtgg ttcaagacga cgaccaccac
     3601 cggtggcaag ctcatcggct tcggtagctc ggcaactggc aacagcggca gctacgaccg
     3661 ccacgtctac atgctgaaca acgggaagct gacgttcggc acctacaccg gggtgaccaa
     3721 cacgatcgcc agctcggcca gttacaacga cggcaagtgg cactacatgg tcgccactca
     3781 ggccagcgac gggatgaagc tgtacgtcga cggcgcgctc gttgcgagcg ggtcggtcac
     3841 cggcgcacag tcctacaacg gttactggcg ggtcggcggc gacaa
//
LOCUS       DATCBJ010000617         2753 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_17851, whole genome shotgun sequence.
ACCESSION   DATCBJ010000617 DATCBJ010000000
VERSION     DATCBJ010000617.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2753)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2753)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2753
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_17851"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(432..932)
                     /locus_tag="VII50_12410"
     CDS             complement(432..932)
                     /locus_tag="VII50_12410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5051695.1"
                     /translation="MSVGFQSDGDGLGLVDGSSSDHVSVGCAGRESGAGVTVGTTSTG
                     CTGEASGGVDAGLSSPRRVALGAPGEGLGVAGRAAASVTSSDSSSTSTGWVGWTNINV
                     DFFDGVAAPELVPVGVGPPISAAAGMDTSTRVPLAYAIAIAAQSTATVPVTGPAHAPI
                     RRVSTR"
     gene            1131..2477
                     /locus_tag="VII50_12415"
     CDS             1131..2477
                     /locus_tag="VII50_12415"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEY5051696.1"
                     /translation="MLGAVVIVFVKQFAASYQTLGARTLTSELRSYTATAAARPPSSD
                     LQTFTVSYLQERALPAGTVLIVSLPGARVATPGSAVLLSDSRVTSWLTDPPTQTFTRA
                     VHVDGSLTEFLVSPIVVGGQRTGTFLAAIDLAGDEGQRSRVLALSVAEAGIALLAGVA
                     SAYLLLRRLLRTVGRITTTAADIGSGALDSRLGDQGIDDEVGQLATTFDSMLERIDTA
                     MTNQRRLLSDVSHQLRTPLTVARGHLDVLKHTGDVANPKVVTETVTLVLDELDHMRSL
                     VERLLLLGRAMEPDFLSPDLIDARAFLADLHSACQVLAPRQWTLAAIPDIVVSADVAK
                     LRGALLNLVDNAVRVTAPNDTIEIACELDNTAGTVTFAIEDSGPGIPAAQREAVLARF
                     ARPGARDQDGSGLGLAIVRAVAEAHGGRVTVGESRLGGARIAMVLPASLVRSLEEV"
     gene            2477..>2753
                     /locus_tag="VII50_12420"
     CDS             2477..>2753
                     /locus_tag="VII50_12420"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY5051697.1"
                     /translation="MHVLIVEDDPRIASFLNKGLRAEGYVTSVATNAIEANTYAEAME
                     TDLNLVLLDLGLPDGSGTGVLQRLRQRQSDVPVIILTARSDVSDKVRG"
BASE COUNT          468 a          894 c          885 g          506 t
ORIGIN      
        1 gtgcgctcga agtcttcagc gttgatttgc ggcaccacct tcgtgggctg ccatgcggcc
       61 gcggcgtagc cggctggttc cccgtgcccg acgttgcgcg catcggggag tcacgatgaa
      121 gaacgatcgc gcacgcctca tcgccaaccc agcgctatgc gacgggcatg ggatctgcgt
      181 actgcgggcg cccgacctct tgaccctaga tgcgtgggga tttgtcggtg tcgagacgac
      241 ggaggcgctg tcaggtcgcg atcttaggcg ggcgcgagtc gcggtcgcgg catgtcccga
      301 gcgggcgctg tcgatggtgg aggtccatgt cacgggtggc aacggcgtcc atcttggagg
      361 aaactcatcg acgtggactc cacctgggcg agagcgcggt cggcgccgag cctcctcaga
      421 caaagacagt tctagcgtgt gctaacacgt cgtattggtg catgggctgg tccggtgacg
      481 ggcaccgtgg cggtgctctg cgctgcgatc gcgatcgcgt acgccagcgg cactcgggta
      541 gaagtatcca taccggctgc ggccgatatc ggcggtccga cgccgaccgg caccaactcg
      601 ggagcggcta cgccgtcgaa gaaatcaacg ttgatgttgg tccatccgac ccatccggtc
      661 gaagtgctcg atgagtcaga cgacgtcacc gaggccgccg cccggcctgc cacgcccagc
      721 ccttcgccag gggcccccag cgccacccgg cgtggcgacg acagcccggc gtcgacgccg
      781 cccgatgcct cacccgtgca cccggtcgaa gtcgtaccca ccgtcacccc cgcaccggac
      841 tcgcgcccgg cgcagccgac agacacatgg tcggacgacg acccgtcaac cagtccgagg
      901 ccgtcgccgt ccgactggaa gccgacagac acaccatcat ggtccccgtc ggggagttac
      961 cgtgacgacg gtagcgagga cgacggtgcc ggcggagcct cgcagtcgga ctcatgatgg
     1021 cggcacgctc tgcacgccat gcacacctcg cacgggcccc gcaacttcat cgtcgacgct
     1081 ttggcaccgc tgctcgactg gcaggtttcc atgcgcttgt gctactcgtc gtgctcggag
     1141 ctgtcgtcat tgtcttcgtc aagcagttcg cggcgagcta ccagactctt ggcgcgcgaa
     1201 cgcttacgag cgagctgcgc tcgtacacgg ccacggcagc tgctcgccca ccgagcagtg
     1261 atttgcaaac tttcacagtg agctacttgc aagagcgagc gttgcctgcc gggacggtgc
     1321 tcatcgtcag ccttcccgga gcgcgtgtgg caacgccagg atcagcggtc ttgctctcgg
     1381 attcacgggt gacgtcctgg ctcacggatc cgccgacaca gacgttcacc agagcagtac
     1441 acgtcgatgg atcgctgacc gagttccttg tctcacccat tgtggtgggt ggtcagcgga
     1501 ccggaacatt tctcgccgca atcgacttag ccggcgacga aggccagcgg tctcgcgttc
     1561 ttgcgctatc ggtggcggag gctggtatcg ccctcttggc gggtgtcgca agcgcttacc
     1621 tgctgctgcg tcgtttgctg cgaacggtag gacgcatcac cacaaccgca gccgacatcg
     1681 gcagtggcgc gctcgacagc aggctcggcg accagggcat cgatgatgag gtaggtcaac
     1741 ttgcgactac cttcgactcg atgctcgagc gcatcgacac ggcaatgacc aatcaacggc
     1801 ggctgctgtc cgatgtgtcc caccaactgc gcacgccact gacggtcgcc cgggggcatc
     1861 tcgacgtcct gaaacacacg ggcgacgttg ctaacccgaa ggtcgtgacc gaaacggtga
     1921 cgctggtgct tgacgagctc gaccatatgc gatctctcgt cgagcgtctc ttgctgctcg
     1981 gccgggcgat ggagcccgac ttcctgtcac ccgatctcat cgatgcccgt gccttcctcg
     2041 ccgatctcca ctcggcatgc caggtgctcg ctccgcgtca atggacacta gcggcgattc
     2101 ccgacatcgt tgtgagtgcc gacgtcgcca aactgcgggg cgctttgctt aacctggtcg
     2161 acaatgccgt tcgggtgact gcgccgaatg acaccatcga aatcgcttgt gagcttgaca
     2221 acacggcggg cactgtgaca ttcgccatcg aagactccgg accgggcata cccgccgcgc
     2281 aacgagaggc ggtgctcgct aggtttgcgc ggcccggcgc gcgggaccag gatgggtcgg
     2341 gtctcggctt ggcgatcgtg cgcgcggtgg cggaagcaca cggcggccgg gtcactgtcg
     2401 gcgagtctcg gctcgggggt gcacgcatcg cgatggtgct tcctgccagc cttgtgcgca
     2461 gcctcgagga ggtgtgatgc acgtcttgat cgtggaggac gatccgcgga tcgcgtcctt
     2521 cctcaacaag ggtcttcgcg ctgaggggta cgtcacctct gtggcgacga atgctatcga
     2581 ggccaacacg tatgccgaag ctatggagac tgacctgaac ctcgtgctct tggatctcgg
     2641 cttacccgac ggatcgggaa caggagttct gcagcgcctg cggcaacgac aatccgatgt
     2701 gccggtgatc atccttaccg cccgctccga tgtgagtgac aaggttcgcg gcc
//
LOCUS       DATCBJ010000618         5650 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_275933, whole genome shotgun sequence.
ACCESSION   DATCBJ010000618 DATCBJ010000000
VERSION     DATCBJ010000618.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 5650)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5650)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5650
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_275933"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..850)
                     /locus_tag="VII50_12425"
     CDS             complement(<1..850)
                     /locus_tag="VII50_12425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
                     /protein_id="HEY5051698.1"
                     /translation="MSAQRDSTDLYLGLIFSVITFFLFLVAGGLLSALLSGHGFPMHF
                     ARGAITAFAHVGDPSFAWKVPVGPAWLYWTMTTIAMIGGGVGIVFTVQFVKHEGRRKT
                     EDPTQLLGLADRHDVVAAAGAKHLVARGSVLRPSVVKPVPNDLGLYLGTSRRVDCYAS
                     VEDSMVILGPPRSGKGFNLVIPFILDAPGAVVTTSTRPDNLAATMTARARRGPVGVFD
                     PQGLARGVSGGLRWSPIRGCEHPQTAMMRASALCAGAAAGVREANFWQQQTEVVVRCL
                     LQAAAVD"
     gene            complement(847..2607)
                     /locus_tag="VII50_12430"
     CDS             complement(847..2607)
                     /locus_tag="VII50_12430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY5051699.1"
                     /translation="MTKRNKQSVASPTRAPIPPIPALLQSRTVWLSDGQQPLTLPKVP
                     GRVRVLTPHPTDEKPVQAILDGGKSLGGALYGEPGTARRAKRARSHAVTKITGADRVA
                     AREDEKRARRQLREEAAAAAYLPRNGEVGGDALRTYRALKVEPHNATSEVLAGAYPFL
                     AESGLGSQGILIGQDAWSGAGFVYDPWVLYQLGILTNPNCLLAGVVGRGKSTLAKSIA
                     TRSIAFGRKCYVPGDPKGEWTAVSRSVGGQAIELGGGTMARLNPLDEGPRPEYLTSQD
                     GVREPVTDTAWFELVQQRRRDLLRSITEAALGRAMAPVESTALHAALDEAVRTNAVPV
                     LPHVVTAMFDPPNDVIGSSRAQLIDDGREVAHAVNRLVGGDLGGLFDGPSTVRFDADL
                     PMVSLDLSRIAGSDAKIGMVMTCASSWMEAALQDPAGGPRWVIYDEAWRVLKQPALLA
                     RMQSQWKLSRALGIANLMVIHRLSDLDAVGDANSESRNLALGLLADCSTKIIYAQERG
                     EDAKTGAALGLSSTEIDQLSSLARGEGLWRIGERSFVVRHVCTPGELALFDTNQRMGQ
                     NPRGFSNSEAFVSGGSGVDQ"
     gene            complement(2616..3113)
                     /locus_tag="VII50_12435"
     CDS             complement(2616..3113)
                     /locus_tag="VII50_12435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5051700.1"
                     /translation="MAVGAVLAGLVALFVIVTLIHPEITRGVFGSPPTTTTVPAATTT
                     VTSTTTTTTPTTDHPITPVAHPVTTTPTPRVAHQINSSIDASSSSPGEVMVSVTVTTD
                     ADRVTGTIYGTSASGDTTATFAGAVVDGTATFNATLDLELPASVYAVVVSSSGTVTTS
                     TVPLS"
     gene            complement(3204..4118)
                     /locus_tag="VII50_12440"
     CDS             complement(3204..4118)
                     /locus_tag="VII50_12440"
                     /inference="COORDINATES: protein motif:HMM:NF013793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5051701.1"
                     /translation="MSRRKDKAAQQTEIERLTAAIATPLLARVPGSHHIVVACPKGGT
                     GKTTTAAMLGLNLADVRGEIVSVVDANPHMSTLRRRLVSPAVPPTRPFVELCARALDE
                     DVLPEWSALAPYTDVVARLRILSNQTADPAEVESLTFAEFEAGIALLRRAAQIVISDM
                     GTSSTGPVAVAAMETANTIVFATELAQDSLELAIEMVSALAGQPLSYRPDPDDWSAVS
                     NGRFAPLVQGAIVAIAPGRGDRDPADLTALIDWLRAVCGGGVFIINKDDHLALGDLIV
                     PSALQSETTIAHLGVAAAVAARFPLAPA"
     gene            complement(4138..5583)
                     /locus_tag="VII50_12445"
     CDS             complement(4138..5583)
                     /locus_tag="VII50_12445"
                     /inference="COORDINATES: protein motif:HMM:NF042935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO6880 family protein"
                     /protein_id="HEY5051702.1"
                     /translation="MTTTTFSPATVRFSRRSTRGLMLGFSASRCIAIGLGALTVLAGL
                     LAGGGPGLLFTAPLWTLLVAIAFVTRKDQPLVEWIPSVLHWSFRVSTKQTAYRARVLA
                     PRPSGTMALPGDAAALRFYNDPQSRTCLIHDPHRQTLSAVLAISHPAYVLLAPIDQVN
                     RVSNWGRVIASLAQTGTCSVLQILESTIPDPGTKVAQWYAAHATHRDDWPNAEYQALL
                     AQSSFGSSTHRTTVTITLDMKRAAAAIRGAGRGVKGAAHVLRIDMSVLEHGLRAADLR
                     LDGWLGEAQVAAMIRQAYDPKTELDANSPGAKLVSAGPVALSEHWSFLHHDSGYSTVL
                     WISEWPRIEVAPHFLHALIFAPDIRKSLCLLARPLGTAEALRALRKEKTEAITDAGHK
                     AKVGQVQDLSDVQEYNDLLKREQALIGGHADVQFSGLITVTASSEEGLTQAVALIERS
                     AGQSGCETRVLYGRQSQGFVVGALPLGRGVF"
BASE COUNT          973 a         1871 c         1825 g          981 t
ORIGIN      
        1 cgtccaccgc agccgcctgc aggaggcaac gcacgaccac ctcggtctgc tgctgccaga
       61 agttcgcctc cctaacccct gcggccgcac cggcgcagag cgcactcgcg cgcatcatgg
      121 ccgtttgggg gtgctcgcat cctcttattg gtgaccaacg cagcccgccg gatactcctc
      181 tcgcgaggcc ctgcgggtcg aagaccccca ccggaccacg cctcgcgcgt gcagtcatcg
      241 tcgccgccag attgtccggc cgggtgctgg tcgtcaccac cgcaccgggc gcgtcgagga
      301 tgaaggggat caccaggttg aaacccttgc cgctccgcgg gggtccgagg atcaccatcg
      361 agtcctcgac gctggcgtag cagtcgacgc ggcgactcgt cccgagatac agtccaagat
      421 cgtttggtac cggctttacc accgacggcc gcaggaccga gccccgggct accaggtgct
      481 tcgccccggc cgcagcgacg acatcgtgtc ggtcggcgag gccgagcagc tgcgtcgggt
      541 cttcggtctt ccggcgtccc tcgtgcttca cgaactggac tgtgaatacg atgccgacgc
      601 caccgccaat catggcaatc gttgtcatcg tccagtacag ccacgcggga ccgaccggca
      661 ccttccacgc gaacgacgga tcaccgacgt gcgcgaatgc ggtgatcgct cccctggcga
      721 agtgcatggg gaacccgtgg ccggagagga gcgccgagag caagccgcca gcaacgagaa
      781 acaggaagaa ggtgatgaca ctgaaaatca gtccgaggta caggtcggtg ctgtcgcgtt
      841 gcgcgctcat tggtccaccc ctgagccccc gctgacaaag gcttcagaat tgctgaaccc
      901 acgggggttt tgtcccatcc tttggttcgt gtcgaacagg gccagctcgc ccggcgtaca
      961 gacgtggcgc acgacaaagg aacgctcgcc aattctccag agtccttcgc cacgagccag
     1021 gctggagagt tggtcaattt ccgtcgagga cagcccgagc gccgcgccgg tcttggcatc
     1081 ttcgcctctc tcttgggcgt agatgatctt ggttgagcag tccgccaaca agcccagagc
     1141 gagattgcgc gactcggagt tagcgtcgcc caccgcgtca aggtcgctca gtcggtggat
     1201 caccatgagg ttcgcgatcc cgagggctcg actgagcttc cactgcgact gcattcgtgc
     1261 gagcagcgcc ggctgcttca acacccgcca cgcctcgtcg tagatcaccc agcgcggtcc
     1321 gcccgcgggg tcttgcaggg ccgcctccat ccacgaactc gcgcacgtca ttaccatgcc
     1381 gatcttcgcg tccgagcccg caatgcggct gaggtcgagg ctcaccatgg gtaggtccgc
     1441 gtcaaagcgc accgtcgacg gtccgtcgaa cagtccgccg aggtcaccgc cgaccagccg
     1501 attgaccgcg tgcgcgacct cacgcccgtc atcaatcaac tgcgcgcgac tggagccgat
     1561 cacgtcgttc ggcgggtcga acatggccgt caccacgtgc gggaggaccg gtactgcgtt
     1621 cgtgcgcacc gcttcatcga gggccgcgtg gagcgcggtg gattcaaccg gcgccatagc
     1681 gcgaccgagg gccgcttcag taatcgagcg caacaggtcg cggcgtcgtt gttgcaccag
     1741 ctcgaaccac gccgtgtcgg tgactggctc gcgaaccccg tcctgacttg tcaggtactc
     1801 gggtctcgga ccctcatcga gcggattgag tcgggccatg gtcccgccac ccagctcaat
     1861 ggcctggccg cccacactcc gacttaccgc cgtccactcg cccttggggt ctcccggcac
     1921 gtaacacttc ctgccgaagg caatggaacg tgtcgcaatt gacttggcaa gggtgctctt
     1981 gcctcggccg actacaccgg cgagcaaaca gttcggattg gtgagaatcc ccaactggta
     2041 gagcacccag gggtcgtaga cgaagccggc gccgctccac gcgtcttgac caatcaaaat
     2101 gccctgacta cccagcccgc tctcggcgag gaacgggtac gcgccagcta ggacttcact
     2161 ggtcgcattg tgcggctcca ctttcagcgc tcgatacgtg cgcagcgcat cgccaccgac
     2221 ctcgccgttc cggggcagat acgccgccgc cgcggcttcc tcgcgcagtt ggcgtcgggc
     2281 tcgcttctcg tcctcgcgcg ccgccacgcg gtcggcacct gtgatcttcg tcaccgcatg
     2341 cgaccgcgcg cgtttggcgc gtctcgcggt gccgggttcg ccgtacagag cgccaccgag
     2401 tgactttcct ccgtcgagga tcgcctgtac gggcttttcg tccgtcgggt ggggtgtaag
     2461 tacccgaacg cgaccaggga ctttggggag agtgagcggt tgctgcccat cggatagcca
     2521 cactgttcgt gactgaagca acgccgggat cggtggaatc ggtgccctcg ttgggctggc
     2581 gaccgattgc ttgtttcgtt tggtcacgtc aactcctaac tgagcggaac cgtggacgtg
     2641 gtgacggtgc ccgaggacga caccactacc gcgtagaccg acgccggcag ttcgagatcg
     2701 agcgtggcgt tgaacgtggc cgtgccgtcc accaccgctc cggcgaacgt ggcggtcgtg
     2761 tcgcccgatg cactggtgcc gtagatcgtt ccggtgacgc gatctgcgtc cgtggtgacc
     2821 gtcacgctca ccatcacttc gccgggactg gacgagctcg cgtcgattga cgaattgatc
     2881 tgatgggcca cgcgaggagt gggcgttgtc gttaccggat gcgccactgg cgttatcggg
     2941 tgatcagtcg tcggcgtcgt cgtggttgta gtactggtga cggtcgtcgt ggccgccggc
     3001 accgtcgtcg tggtcggcgg cgacccgaac accccgcgcg tgatctcggg atggatgagc
     3061 gtcacgatca cgaacagtgc caccagcccc gcgaggaccg ccccgaccgc catccagagc
     3121 gtccggcgtg tccacgcgcg ttcggccggc tgcggagtgg gattccactc ttcgggcctc
     3181 ggaggtagct cgggtagcac gaactacgcc ggggccagtg ggaaccgcgc ggccacggcg
     3241 gcggccacgc cgaggtgcgc gatggtcgtt tccgactgca gcgcgctggg aacaatcaga
     3301 tcacccaatg ccaggtgatc gtccttgttg ataatgaaga ccccgcctcc gcacacggcg
     3361 cgaagccagt cgatcaacgc agttaaatct gcgggatcac ggtcgccgcg accgggggcg
     3421 atggccacga tcgccccctg caccaagggc gcgaaccggc cgttcgacac ggccgaccag
     3481 tcgtcaggat cgggccggta actgagcggt tgaccggcca acgcgctcac catttcgatt
     3541 gcgagctcga gggagtcttg cgccaattcg gtcgcaaaga caatcgtgtt cgccgtctcc
     3601 atcgccgcga cggcgaccgg gccggtggac gacgtaccca tgtccgagat gacgatctgg
     3661 gccgcgcggc gaagcagcgc aattccggcc tcgaactctg cgaaggtgag actctcaacc
     3721 tccgccgggt cggccgtctg attgctgaga atccggagtc gagcgaccac gtcggtgtac
     3781 ggcgccagag cggaccactc gggcagcacg tcctcgtcga gggctcgggc gcacagttcc
     3841 acgaacggac gcgtcggggg cacggcgggc gacaccagac gtcgacggag cgtactcatg
     3901 tggggattcg cgtccacgac gctcacgatc tctccgcgca cgtcggcgag gttgaggccc
     3961 aacatcgccg ccgtcgtggt cttgccggtg ccccccttcg ggcacgcaac cacgatgtga
     4021 tgcgagccgg gcacgcgagc caacaacggc gtggcaatcg ccgccgtcag ccgttcgatc
     4081 tcggtctgct gtgctgcctt atctttacgt cgtgacatgt cgtaccaccc ttttctgtta
     4141 gaagacaccg cgaccgaggg gcaacgcccc gacaacaaaa ccctgcgact ggcgcccgta
     4201 gagcacccgt gtctcgcacc ccgactggcc agcggagcgt tcgatcagcg cgacggcctg
     4261 agtgagtccc tcttcgctgc tcgcggtgac ggtgatcagt ccgctgaact gcacgtcggc
     4321 gtgaccgccg atcagggcct gctcgcgctt tagcaagtcg ttgtactcct ggacgtcgga
     4381 gaggtcctgc acttgaccga ccttggcctt gtgcccggcg tcggtgatcg cttcggtctt
     4441 ctccttgcgt agcgctcgca gagcttcggc cgtgcccagg ggccgggcga gcaggcacag
     4501 cgactttcga atatcggggg cgaagatgag cgcgtgcagg aagtgtggcg ccacctcgat
     4561 cctgggccac tcgctgatcc acaggaccgt cgaatatccc gagtcgtggt gcaagaagga
     4621 ccagtgctcc gagagggcta ccggtcccgc cgagacgagc ttcgctcccg gactgttcgc
     4681 gtcgagctcg gtctttggat cgtaggcctg gcggatcatc gcggcgacct gcgcctcgcc
     4741 cagccagccg tcgagacgca ggtcggccgc gcgcagcccg tgctcgagca cggacatgtc
     4801 gatgcgcagc acgtgcgcgg cgcccttgac tccgcgtccc gcgccacgaa tggccgccgc
     4861 ggctctcttc atgtcgaggg taatggtgac ggtcgtgcga tgagtggagg atccgaacga
     4921 cgactgggcg agcaacgcct gatactcagc gttgggccag tcgtctcggt gcgtggcgtg
     4981 cgcggcgtac cactgcgcga ccttcgtacc ggggtcggga atcgttgact ccaagatctg
     5041 cagcaccgag cacgtgcctg tctgggcgag ggacgcgatg acgcgccccc agtttgacac
     5101 ccggttcacc tgatcgatcg gagccagcag gacgtacgcc ggatgggaga tggcgagcac
     5161 cgccgacaac gtctggcgat ggggatcgtg aatcaggcac gttctcgatt ggggatcgtt
     5221 gtagaaccgc aacgccgccg cgtcgcccgg tagggccatc gtgcccgacg gacgtggcgc
     5281 cagcaccctg gcccgatagg ccgtctgttt cgtcgacact cgaaacgacc agtgcagtac
     5341 cgatggaatc cattcgacga gaggctggtc cttgcgcgtg acgaacgcaa ttgctacaag
     5401 cagcgtccat aggggcgcgg tgaagagtag ccccgggccg ccgcctgcga gcaggcccgc
     5461 cagcaccgtc agcgcgccca gacctatcgc gatgcaacgc gatgccgaaa agccgagcat
     5521 caacccgcgc gtggaacgtc gggaaaatcg cactgtggcc gggctgaaag tggtagtggt
     5581 cacgatctac tgcttttgca actcgggagg actgacggga aacggtggtg ccggcacgga
     5641 cgaagatgct
//
LOCUS       DATCBJ010000619         6801 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_395284, whole genome shotgun sequence.
ACCESSION   DATCBJ010000619 DATCBJ010000000
VERSION     DATCBJ010000619.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 6801)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6801)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6801
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_395284"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(122..3499)
                     /locus_tag="VII50_12450"
     CDS             complement(122..3499)
                     /locus_tag="VII50_12450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="error-prone DNA polymerase"
                     /protein_id="HEY5051703.1"
                     /translation="MGFNNPDIPWRELERQLSGRPKPAPTPGYKPVDGDGGDSPAWSR
                     KRERYQPVSPPRADGSTQPAVPYAELHCHSNFSFLDGASEPEELAEEAARLGIETLAI
                     TDHDGFYGVVRFAEAAGELGVATVFGAELSLGLRTPQNGIADPEGDHLLVLARDPEGY
                     RRLSMAISAAQLAGKEKGRPIYDLEELGDSHGGHWVVLTGCRKGSVPKTLAAEGFHAA
                     EAELDRLAALFGRDNVFVELTDHGNPLDSTRNVALFDLAGRRKLGVVATTNAHYATPG
                     GGRLAAALAAVRGRRGLDDMDGWLPAAGTAHLRSGSEMAARFARYPGAVDLAAEIGRA
                     CAFDLRLVAPRLPDFPVPAGHSEMSYLRELAMQGALKKYGSPDAERQLGAYKQLEHEL
                     EVIEQLNFPGYFLIVWEIVEFCRANDILCQGRGSAANSAVCYAIGIAKADPVSFHLLF
                     ERFLSPERDGPPDIDLDIESGRREEVIQHVYTRYGRTNTAQVANVISYRPKSAVRDVA
                     KALGYSTGQQDAWSKQIDSWGSVTSDASGTGAGTDHDIPGDVIELAEQVLRFPRHLGI
                     HSGGMVICDRPVTEVCPVEWGRMENRTVLQWDKDDCARTGLVKFDLLGLGMLSALHGA
                     FDLLREHYDVDLQLDTVPPEDPKVYDMLCAADSVGVFQVESRAQMATLPRLKPREFYD
                     LVTEVAIIRPGPIQGGSVHPFIRRRNGLEEATYAHPLLKNSLERTYGVPLFQEQVMQM
                     AIDVAGFTASESDELRQAMSSKRSSARMERLRGKLYGGMAERGITGAVADEIYEKLTA
                     FAHFGFPESHSISFAYLVYASAWLKLHYPAAFCAALLNAQPMGFYSPQSLVADARRHG
                     VEVRRPDINASAALTTLEPVDARAHSKSGAHSKSGMHSGARAVRLGLTSVRGITDPLG
                     ERIAAGRPYADMTDLVHRVGLAAVQVEALATAGAFDCFGMSRRVALWAAGAAAEGSPD
                     RLAGTVLGTHAPTLPVMSDVEVTVADLWATAITTDGYPTRHVRDRLDELGVLTASALR
                     TAEAGRRVLVGGVVTHWQRPATAGGTTFLNLEDETGMVNVICSRGVWIHYRRVARTAA
                     ALLVRGRLEREQGVVNVVAERFERLPLAVRRTSRDFR"
     gene            complement(3544..5145)
                     /locus_tag="VII50_12455"
     CDS             complement(3544..5145)
                     /locus_tag="VII50_12455"
                     /inference="COORDINATES: protein motif:HMM:NF013015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase Y family protein"
                     /protein_id="HEY5051704.1"
                     /translation="MTVAAGFVPQVRVLAVWCPDWPVIAAGGSAELPGAVMHANRVVA
                     CSAAARLDGVRRGQRRRDAQSKCPELTVYSHDPGRDARLFESVAVAVEAIVPGIEIIR
                     PGLLVVAARGAARYYQGELPLLATIARAVSDATRYDVQLGIADGIFAATQAAYAASIV
                     PVGVAAAFLAPLPIRVLDRPELADLLTRLGIHTLGDFADLPRTDVLARFGPDGAYVHD
                     LARGIEMRPPSARVVPPELAVEMTLDPPVDRVDTATFVAKTLAEQLHERLAARGLACI
                     RISIEACTENGEQLSRSWRHGSISPAVSTPGFTANAIADRTRWQLDGWLSGTIRSDDG
                     QPSSGLVFLRLVPDEVIYDDGRQLSLWGAEAGGASGPGGDLEQIDRALARVQGMLGPD
                     AVVTAVVGAGRDVAEQIRLVSWGEPREVLPADAWPWPGRLPAPSPATVLKSAQPAAVT
                     DVGGNPVVVTGRCVVSAAPGWLRIGQQPPVAITGWAGPWPLDVRWWDRLTARRRARFQ
                     IATVDGAGLLMVCENGRWSVEALYD"
     gene            complement(5142..5762)
                     /locus_tag="VII50_12460"
     CDS             complement(5142..5762)
                     /locus_tag="VII50_12460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5051705.1"
                     /translation="MLRSAASSLEGLSPDLLEQLRGTGSKELLPVPQALAELFPEGGV
                     RRGSTITVSGSSSLVLALLAEVSQHQGWCAEVGSPLLGSAAAAEAGVELERFVRVAEP
                     GEQWPSVVASLLEAFDIVVIHPPPRAREADMRRLSARARERSAVLLTTAKWEGSQIGL
                     SVVEQRWHGLGTGHGYLQAHELEVQSLGRGAAVRPRRATVWLPQAL"
     gene            5833..6321
                     /locus_tag="VII50_12465"
     CDS             5833..6321
                     /locus_tag="VII50_12465"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY5051706.1"
                     /translation="MAINETASDVVLRVVRWDDPHAEELRAAQRADIVARYGGDFEPG
                     HRPTAADIAVFVVAYDGATPIGCGAFRDLVDEQPTVEVKRMYVRPEYRGRKIGQLILG
                     DLETRARERGATRVRLETGTRQPEAVRLYEGAGYRLIPNFGDYVDAPASLCFERILEA
                     TE"
     gene            6318..6794
                     /locus_tag="VII50_12470"
     CDS             6318..6794
                     /locus_tag="VII50_12470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HEY5051707.1"
                     /translation="MSDLHPGVVRVQDALLAAGAKGSVRMLDESARTAAEAAAALGVE
                     VGQITNSLVFVADGEPLLVLTSGRHRVDTSKVAELVGVTKVSRADADFVREHAGVAIG
                     GVSPVGHPRPLRTLVDVALNDYQVVWAAAGHTHAVFPTSFEELLTLTGGTPAEVGD"
BASE COUNT         1300 a         2238 c         2166 g         1097 t
ORIGIN      
        1 cacgcgactt tcgctgaatg ggcttgacgg atcctcgagt ggccgcttac tgtggactcg
       61 ttcgaacata tgttcgaacg agtccacagt aagcggccac tcgaggatcc gtcaagccca
      121 ttcagcgaaa gtcgcgtgac gtcctgcgga ccgccagcgg cagtcgctcg aaacgctcgg
      181 caaccacatt gacgacgccc tgctcacgct cgagacgacc tcgcaccaag agagcagcgg
      241 cggtgcgggc cacccgtcgg tagtggatcc agacgccgcg cgaacaaatc acgttcacca
      301 tgccggtctc gtcctcgagg ttgaggaagg tggtcccacc cgcggtcgcc ggccgttgcc
      361 agtgggtcac gactcccccg accaggacac ggcgtccggc ctctgcagtg cgtagggcgc
      421 tggccgtgag cacgccgagt tcgtcgagac gatcgcgcac gtggcgggtc ggatagccgt
      481 cggtcgtgat cgcggtcgcc cacaggtcgg ccacggtaac ctcgacgtcg ctcatcacgg
      541 ggagagtggg agcgtgcgtt ccgagcaccg tgccggcgag gcgatcagga gacccttccg
      601 ctgcagcgcc agcggcccac aacgccaccc gccgactcat cccgaaacag tcgaaggctc
      661 cggcggtcgc cagtgcctcg acctgaacag ccgctagccc gacccgatgc actaagtcgg
      721 tcatgtctgc gtaagggcga cccgccgcga tgcgctcgcc aaggggatcg gtgattccgc
      781 gcacagaagt cagcccgagc cgaactgcgc gcgcaccact gtgcatccct gacttactgt
      841 gcgcccctga cttactgtgc gcccgtgcat cgactggttc gagagtggtc agggctgcac
      901 tcgcattgat gtcgggacgc cgcacctcaa cgccatggcg acgcgcgtca gcaaccaacg
      961 actggggcga gtagaagccc atcggctgcg cattgagcag tgcggcacaa aaagctgccg
     1021 ggtagtgcaa tttcaaccat gcgctggcat agacgagata ggcgaagctg atcgagtgac
     1081 tttcgggaaa accgaagtgc gcgaatgcag tgagcttctc gtagatctcg tcggcgacgg
     1141 cgcccgtgat gccacgctcg gccattccgc cgtagagctt gccgcgcagc cgttccatgc
     1201 gagcgctcga tcgcttcgaa ctcattgcct gccgcaactc atctgactcg gacgccgtga
     1261 agccggcgac atcgatcgcc atctgcatca cttgctcttg gaagagtgga acgccgtagg
     1321 tacgttcgag cgagttcttc aacaatggat gggcataggt ggcctcttcg agaccgttgc
     1381 gccggcggat gaagggatgt acggagcccc cctgaatggg cccaggcctg atgatcgcga
     1441 cctcggtgac cagatcgtaa aactcccgcg gtttcaagcg cggcaacgtg gccatctgcg
     1501 cacggctctc gacctggaac accccgaccg agtcggcggc gcacaacatg tcgtagacct
     1561 tcggatcttc gggcgggacg gtgtcgagct gcaggtcgac gtcgtagtgc tcgcgtagca
     1621 gatcgaacgc accgtgcaag gcggacaaca tgcccagacc caacaggtcg aacttcacca
     1681 aaccggtgcg cgcgcaatca tctttgtccc actgcaaaac cgtgcggttc tccatgcggc
     1741 cccactcgac aggacacacc tcggtgaccg gccggtcgca gatgaccatg ccaccggaat
     1801 gaatgccgag atgcctcgga aagcgcagga cctgctcggc cagttcgatg acatcgccgg
     1861 ggatatcatg gtcggtacct gcaccggtgc cactcgcatc ggaggtgacg gatccccagc
     1921 tgtcgatttg ttttgaccac gcatcttgtt ggccagtcga gtatccgagc gcctttgcca
     1981 cgtcgcggac ggccgacttc ggccggtagc tgatcacgtt ggccacctgc gccgtattcg
     2041 tacgtccata gcgcgtatag acgtgttgga tgacttcttc gcggcgtccg ctctcgatgt
     2101 cgagatcgat gtcgggcggg ccgtctcgct cgggtgaaag gaaccgttcg aacagcagat
     2161 ggaaggacac cggatcagcc ttggcgatac cgattgcgta gcacactgcc gagttcgccg
     2221 ccgagccacg gccttggcac aagatgtcat tggctcggca gaactccacg atctcccaga
     2281 cgatcaggaa atagccgggg aaattgagct gctcgatcac ctcgagttcg tgttcaagct
     2341 gcttgtaggc accgagctgt cgttcagcat caggtgaccc gtactttttc agcgcaccct
     2401 gcatcgcaag ctcacgtagg taactcatct cggaatgtcc tgcgggcacg gggaaatccg
     2461 gcaagcgcgg cgcaacgagc cgcagatcga aagcacaggc gcggccgatc tcggcggcga
     2521 ggtctaccgc accgggataa cgcgcgaacc tcgccgccat ctcagaacct gagcgcaggt
     2581 gcgcggtgcc ggcggcaggc agccaaccgt ccatgtcgtc aagaccacga cgaccgcgga
     2641 cggcggccag tgccgcagcg agccgaccgc cgccaggagt ggcgtaatgc gcgttagtgg
     2701 tggcaaccac gcccagcttt cggcgtccgg ccaaatcgaa gagcgcaaca ttccgagtgg
     2761 aatcgagcgg attgccatga tcagtcagtt cgacgaacac gttgtcgcga ccgaacaatg
     2821 cggcgagccg gtcaagctca gcttcagcag catgaaaccc ttctgcggca agggttttgg
     2881 ggacactacc tttgcggcaa ccagtgagaa cgacccagtg accgccgtgc gaatccccca
     2941 gttcttcgag gtcgtagatt ggccgacctt tctccttgcc ggcgagttgc gccgcactga
     3001 tagccatcga gagccggcga tacccttccg ggtcacgcgc aaggacgagc agatggtcac
     3061 cttcaggatc ggcgatgccg ttttgcggtg tgcgtagacc gagactaagt tcggcaccga
     3121 acaccgtggc gacgccgagt tcaccggccg cttcggcgaa gcgcacgaca ccgtaaaacc
     3181 cgtcgtggtc ggtgatggcg agggtttcga tcccgagtcg tgccgcttct tcggcgagtt
     3241 cctcgggttc gctggcgccg tcaagaaagc tgaagttgga atggcagtgc agttcggcgt
     3301 acggcaccgc tggctgcgtc gacccgtcgg ctcgaggtgg cgagactggc tggtaacgct
     3361 cgcgtttgcg tgaccaggcc ggactgtcgc caccatcacc gtcgaccggc ttgtaaccag
     3421 gagtgggtgc cggcttaggc cgccccgaca gttggcgttc gagttcacgc cacgggatgt
     3481 cggggttatt gaagcccaca gcgacctccc tgaccgttag tcatgtcgcg cctgctgcgc
     3541 gcgtcagtca tagagggcct cgaccgacca gcggccgttc tcgcacacca tcagcagacc
     3601 ggcaccatcg acggtcgcaa tctggaaacg cgcacggcgg cgcgccgtca ggcggtccca
     3661 ccagcggacg tcgagtggcc agggtccggc ccaaccggtg atcgccaccg gtggttgctg
     3721 cccgatgcgc aaccagcccg gggccgcgga gacgacgcac cggccggtca ccaccacggg
     3781 gttgccacct acatcggtga ccgcggccgg ctgtgctgac ttgagcaccg tggctggcga
     3841 gggtgcaggc aaccgacccg gccacggcca cgcatcggcg ggcaatacct cgcgaggctc
     3901 accccacgac accagcctga tctgttcggc cacatcgcgg ccggctccga caaccgcggt
     3961 gacgactgca tcgggcccga gcatgccttg aacgcgagcg agcgcgcgat cgatctgctc
     4021 aaggtcgcca cccgggccac tcgcgccacc cgcttcggcg ccccacaaag aaagctgccg
     4081 gccgtcgtca tagatgactt cgtcgggcac caaccgcaag aacaccaaac cgctcgacgg
     4141 ctgtccgtca tcggaccgga tggtgcccga caaccagccg tcgagttgcc agcgggtgcg
     4201 atcagcaatg gcattcgcgg taaaacctgg ggtggagacc gccggagaaa tactcccgtg
     4261 ccgccaactg cgcgacagct gttcaccgtt ttcggtgcac gcttcgatac tgatccgaat
     4321 gcaggcaagc ccccgcgcag ctagccgctc atgcaactgt tcagccagcg tcttggcgac
     4381 gaaggtggcg gtgtcgacac ggtcgaccgg agggtcgagc gtcatctcga cagcgagttc
     4441 gggcggcacg acgcgagccg atggtggtcg catctcgata ccgcgggcga gatcgtgcac
     4501 gtaggcgccg tcaggcccga acctggcaag cacatcggta cgcggcaagt cggcgaaatc
     4561 accgagcgta tggatgccga gtctggtcag cagatcggcg agttcaggcc ggtcgagaac
     4621 ccggatcggc agcggcgcaa ggaaagccgc cgccacaccg acaggcacga tggacgccgc
     4681 ataggcagcc tgggtagcag cgaagatgcc atcagcaata ccgagttgga cgtcgtatct
     4741 ggtcgcatcg gaaacagctc gcgcaatggt ggcgagcaac ggcaattccc cttggtaata
     4801 gcgggccgcg ccccgcgcag ccacgacgag cagcccaggt cggatgatct cgatgccggg
     4861 aacgatcgct tcgacggcaa ctgccaccga ctcgaaaagc cgagcgtcac ggccagggtc
     4921 atggctatag accgtcagct caggacactt cgattgggca tcacgacgtc gctgaccgcg
     4981 gcgcactccg tcgaggcgag cagcggccga gcaagcgacg acgcggttcg cgtgcatgac
     5041 ggcacccggc agctcggcgc taccgccggc agcgatcacc ggccagtcag gacaccagac
     5101 agcaagaacg cgaacctgtg gcacaaagcc tgcggcaact gtcataaggc ctgcggcaac
     5161 cagactgtgg cacgccgcgg ccggaccgcc gccccacgac cgagcgactg cacctcaagt
     5221 tcgtgggcct gcaggtatcc atggccggtc ccgaggccgt gccagcgctg ctcgacaacc
     5281 gaaagcccga tctgactgcc ttcccacttg gcggttgtca gcagcaccgc ggagcgttcg
     5341 cgtgccctgg cgctcagccg gcgcatatct gcttcgcggg cccgaggtgg cggatggatg
     5401 accacgatgt cgaacgcctc gagcaggctg gcgacgaccg agggccactg ctcccccggc
     5461 tcggctaccc ggacgaacct ttcaagctcg actccggcct ccgccgctgc ggcactgccg
     5521 agtaacggcg aaccgacctc tgcacaccag ccctggtgct gggagacctc cgccagcaac
     5581 gccagcacca gagacgacga gccgctcacg gtgatggtcg aaccgcgccg cactccccct
     5641 tcggggaaaa gctcggccaa cgcctgcggc accggcaaaa gctccttgct gccggtgccg
     5701 cgaagctgct cgaggaggtc tggcgacagg ccctcgagtg acgatgcggc ggagcggagc
     5761 atcccttgag tatcgaacat atgttcgaac gccgtcaatg caggccgctc gcgggatctc
     5821 taggatcggc gggtggcgat caacgaaacg gccagcgacg tcgtcctgcg tgttgtgcgg
     5881 tgggacgacc cccacgccga ggagctgcgc gctgcccagc gcgccgacat tgtggcccgc
     5941 tacggcgggg atttcgaacc tgggcatcgt ccgacggccg ccgacatcgc agtgtttgtg
     6001 gtcgcctacg acggcgcgac acccatcggg tgcggggcct ttcgcgatct ggtcgacgag
     6061 cagccgaccg ttgaggtcaa gcggatgtat gtgcgaccgg agtatcgcgg ccgaaagatc
     6121 ggccagctca tcctgggcga ccttgaaacg cgcgcccgtg agcgaggggc gacgcgcgtc
     6181 cgactcgaga ccggtactcg gcagcccgaa gcagtgcggc tctatgaagg cgccggctac
     6241 cggctgatcc cgaatttcgg cgactacgtc gacgccccag ccagcctctg cttcgaacgc
     6301 atcctggagg caaccgaatg agcgacctgc acccgggcgt cgtacgcgtt caagatgcgt
     6361 tactcgcagc gggcgcaaag ggcagtgtgc gcatgctcga cgaatcggcc cgcacagcag
     6421 ccgaagctgc cgccgcctta ggcgtcgaag tagggcagat caccaacagc ctggtctttg
     6481 tagcggacgg cgaaccgttg ctcgtcctga ccagcggccg tcatcgggtt gacacctcga
     6541 aggtggctga gcttgtgggc gtgaccaagg tcagccgcgc cgacgcggac ttcgtccgcg
     6601 aacacgccgg agtagcgata ggcggtgtct cccccgtcgg tcacccacgt ccgctccgga
     6661 cgctcgtcga tgtcgcgttg aacgactacc aggtggtgtg ggccgcggcc ggccacacgc
     6721 acgccgtctt cccgaccagc ttcgaggaac tgctcaccct caccggcggc acacccgccg
     6781 aggtcggcga ttaaggcaga a
//
LOCUS       DATCBJ010000620         2286 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_44753, whole genome shotgun sequence.
ACCESSION   DATCBJ010000620 DATCBJ010000000
VERSION     DATCBJ010000620.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2286)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2286)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2286
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_44753"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            144..1787
                     /locus_tag="VII50_12475"
     CDS             144..1787
                     /locus_tag="VII50_12475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009077635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HEY5051708.1"
                     /translation="MSELHPTALDRQRWWPGAAIYQVYIRSFADGDGDGTGDIAGVRS
                     RLPYLADLGIDAIWFNPFYVSPMADAGYDIADYRNVEPLFGSLADAEALIAEAHALNV
                     RVIIDVVPNHGSDAHVWFQAALAAGPGSAERNLFWFRPGRGENGELPPNDWNSIFGGP
                     AWTRVTEADGSPGEWYLHLFAPKQPDFNWENPAVRAEFLDVLRFWLDRGADGVRIDSA
                     ALLAKDPLLREVPPDESGEQHPFIDLDEVHEVYREWRQLTDSYPGDRILIGEVWLPDR
                     ERFTRYLRPDELHTAFNFYFLGCAWEADRIRSVIDDTLADHAPVGAPPTWVLSNHDVV
                     RHVTRYGRGDTSFSMDNRRLGEFSDLELGTRRARAAALVTMALPGAVYVYQGEELGLW
                     EVEDLPPALLQDPIWSRSGYTDRGRDGCRVPIPWDGDSSPFGFGPSGSEPWLPQPAGW
                     KTLTVAAETGDPESMLELYRAALHIRRTVASLGPGPLSWLPSADGVVTFARGTDFVCI
                     ANTSQQPVLLPEHDAVLLASAPIVNSLLPADAAAWLRIC"
     gene            1781..>2286
                     /locus_tag="VII50_12480"
     CDS             1781..>2286
                     /locus_tag="VII50_12480"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEY5051709.1"
                     /translation="MLKRVLVTGAGGRLGRHVLSACADAGIATKALALARDATAAIAA
                     DHVVIGDACDPLVVSDAVADVDAVVHLAAIPSPHGGTAEEVFVGNTSATFTVLEQAAQ
                     AGVRRAVIASSHAATGMPFAASVRRPAYLPIDEHIPLQVEDPYALSKEVDELTAAMMW
                     WRHNLSVVA"
BASE COUNT          378 a          731 c          741 g          436 t
ORIGIN      
        1 attgcctcat tgccgatgat catcttcttc ctgattttcc agcgcaacat catgtccgga
       61 ctcaccgcag gtggcgtcaa ggggtgatct cgcgagctcc agatgttcga cagtaaagga
      121 tcacgaatga agggattgcc acggtgtccg agctccaccc gactgccttg gaccgacaga
      181 gatggtggcc aggcgctgcc atttaccagg tctacatacg cagcttcgcc gacggtgacg
      241 gagacgggac gggcgacatc gcaggtgtgc gatcacgatt gccgtatctg gccgacctgg
      301 gtatcgacgc catctggttc aaccccttct atgtgtctcc catggccgac gccgggtacg
      361 acatcgccga ctaccgcaac gtcgagccgc tgttcggttc gcttgcggac gcagaagccc
      421 tcattgccga ggcgcatgcg ctgaacgtca gagtcatcat tgacgtcgtc cccaatcatg
      481 gttccgacgc gcacgtgtgg ttccaggctg cgcttgccgc gggtcctgga tcggccgagc
      541 gcaacctgtt ctggtttcgg cccggtcgtg gcgaaaacgg cgagctgccg cccaacgact
      601 ggaactccat cttcggtggg cccgcatgga cgcgagtcac cgaagcggac ggcagcccgg
      661 gggagtggta cctgcatctg ttcgcaccga agcagcccga cttcaactgg gagaaccctg
      721 ccgtcagggc tgagtttctg gacgtgctgc gcttctggct ggatcgcggt gctgacgggg
      781 ttcgcatcga ctccgcagcg ttgctcgcca aggatccgct gttgcgtgag gtaccacccg
      841 acgagtcggg ggagcagcat ccgttcatcg atctcgatga ggtccatgag gtctatcgcg
      901 agtggcgtca gctcaccgac agctaccccg gtgaccgaat cctgatcggt gaagtgtggt
      961 tgcccgaccg cgagcggttc actcgttacc tgcgccctga tgagctgcac acggcgttca
     1021 acttctactt cctcggctgc gcatgggaag ccgatcgcat tcgcagcgtg atagacgaca
     1081 cgctcgccga tcacgcgcca gtcggcgcac ctccgacatg ggtgctgtcg aatcacgacg
     1141 tcgtgcgtca cgtcacgcgc tatggccgcg gggatacatc gttttccatg gacaaccgcc
     1201 gtctcggaga gttcagcgat ctcgaactcg gaacacgacg ggctcgagcg gccgcgttgg
     1261 tcacgatggc gttgcccggc gcggtctacg tctatcaggg agaggagctc gggctctggg
     1321 aggttgagga cttgcccccc gcgctgctgc aagacccgat ctggtcgcgg agtggctaca
     1381 ccgatcgcgg gcgcgacggt tgccgcgtgc cgatcccctg ggacggcgac agctcgccgt
     1441 tcggttttgg accgagcggc agcgagccct ggctgccaca gccggccgga tggaagaccc
     1501 tcacggtcgc agcggagacc ggcgatccgg agtcgatgct cgagttgtat cgggcggcac
     1561 tgcacatccg ccgaactgtt gcctcgctcg gtcctggacc gttgtcgtgg cttccgtcgg
     1621 cggacggcgt cgtcacgttc gcccgaggga ccgacttcgt atgtatcgcg aacacgtcgc
     1681 aacagccggt cttgctaccc gagcacgacg cggtcttgct cgcgagcgcg ccaatcgtta
     1741 actccttgtt gcccgccgac gcagctgcgt ggttgcggat ttgctgaagc gagtgctggt
     1801 cacaggagcg ggcggtcggc tggggcgcca tgtcctttcc gcctgcgcgg acgccggtat
     1861 cgcgacaaag gcgctcgcgc tggctcgtga cgcgacggcc gcgatcgcgg ccgatcacgt
     1921 cgtgatcggc gatgcatgtg atccgctcgt ggtcagcgat gccgtcgcgg atgtcgatgc
     1981 cgtcgtgcac ttggcagcca tcccctctcc ccacggcggc accgccgagg aggtatttgt
     2041 cggcaacacc agcgcgacct tcacggtgct cgagcaggcc gcccaagcag gtgtgcggcg
     2101 cgcggtgatc gcgagcagcc atgccgcaac cgggatgccg tttgccgcat cagttcgccg
     2161 gcccgcgtac ctcccgatcg acgagcacat cccactccag gtcgaggatc cgtatgcgct
     2221 ctcaaaggag gtcgatgaac tgaccgcggc gatgatgtgg tggcggcaca acctcagcgt
     2281 ggtcgc
//
LOCUS       DATCBJ010000621         2280 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_92355, whole genome shotgun sequence.
ACCESSION   DATCBJ010000621 DATCBJ010000000
VERSION     DATCBJ010000621.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2280)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2280)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2280
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_92355"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            143..1225
                     /gene="paaE"
                     /locus_tag="VII50_12485"
     CDS             143..1225
                     /gene="paaE"
                     /locus_tag="VII50_12485"
                     /EC_number="1.14.13.149"
                     /inference="COORDINATES: protein motif:HMM:TIGR02160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaE"
                     /protein_id="HEY5051710.1"
                     /translation="MAGRPQFHTVRVSAIDRLTTDAVAITFAVPDDLAGEYAFSPGQH
                     VTVRCAAAGDDGRRTYSLCSVPGVVRIGVKQIPGGVFSTYALGGLRIGDDIEVMTPTG
                     RFTTQVDGARPRHRAAIVAGSGITPVLSIAAATLRAEPASWFTLIYGNRSADAVMFLD
                     ELADLKDRFPDRLAVFHVLSREVRDSALLTGRLDRAKLDELLEVAVAPGTVDEWFVCG
                     PAAMIDAARAALREHDVDDATVHVELFHLATATRSTDMQAAVADTLTEQQASASVTIT
                     LAGRSTTLAVPPGRDVLHEVMRVRPDVPYGCTNGMCGTCRAKVVEGRVEMDHCYALDQ
                     GELDAGFVLTCQAQPRSEVLVLDYDA"
     gene            complement(1213..1803)
                     /locus_tag="VII50_12490"
     CDS             complement(1213..1803)
                     /locus_tag="VII50_12490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5051711.1"
                     /translation="MESEVLAATSFWRAVALLRLERPTGLAALESVFAGGQPAARLRG
                     PMRGRFLASTIGHHIDPAFEALTSMWLPWKGKTFDPDAEQGRNLFTAGARRLMRVTLP
                     NYATIDEAGGCSAFRFLTSIGPSAFTPGVDVLRIDYRDVPENPSWPVRKILDELVMIE
                     DGRYLGQALIEFRGSLRRAAWFSLEDLHIVLTDYAS"
     gene            1883..>2280
                     /locus_tag="VII50_12495"
     CDS             1883..>2280
                     /locus_tag="VII50_12495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5051712.1"
                     /translation="MADGDFVQLAETISPGRRLGRHVEHDARSRNFPAAVVPIADVTH
                     VRHGEPFDQGQLGSCTGNAMAGALMTAPYYQSGRTLVEASAVQLYEQATHLDNVQGSY
                     PPDDTGSTGLAVAKAAKRDGYISAYSHAFG"
BASE COUNT          379 a          768 c          749 g          384 t
ORIGIN      
        1 gcgcttgctg tgcgttgccc gcggtgcgat tcgctcgaca cgagactggt cagccgtttc
       61 ggctcgaccg cctgccaggc gatgtatcga tgcacctcgt gcctcgaacc gttcgacgcc
      121 atcaagccgt actgagcttt acgtggcagg gcgtccgcag tttcacaccg ttcgagtgtc
      181 cgccatcgac cgactgacca ccgacgctgt cgccatcacg ttcgcggtac ccgatgacct
      241 tgccggcgag tacgccttct cgccgggtca gcatgtgacg gtccgctgtg ccgcggcggg
      301 tgacgacggt cgtcgcacct actcgttgtg ctcggtacca ggcgtcgtgc gcatcggcgt
      361 caagcaaatt cccggcggcg tcttctccac ctatgcgctc ggcggcttgc gcatcggcga
      421 tgacatcgag gtcatgactc cgaccggccg gttcacgacg caggtcgacg gggcacgacc
      481 gcgtcaccgc gccgcgatcg ttgcaggtag cggaatcacg ccggtgttgt cgatcgcggc
      541 tgcgacgctg cgcgccgagc ccgccagctg gttcacgctg atttatggca accgcagcgc
      601 cgacgccgtc atgttccttg acgagctggc cgacctgaag gatcgctttc cagaccgact
      661 cgcggtcttc cacgtcctgt cccgtgaggt ccgcgactca gcgctgctca cgggccgtct
      721 cgatcgcgca aagctcgatg agctactcga ggttgcggtc gcgccgggca ctgttgacga
      781 gtggttcgtg tgtggcccgg ccgcgatgat cgacgccgca cgtgcggcgc tgcgcgagca
      841 cgacgttgat gacgccaccg tgcatgtcga gctcttccat ctcgcgaccg cgacccgttc
      901 gaccgatatg caagccgccg tcgccgacac gctgaccgag cagcaggcga gcgcgagcgt
      961 gacgatcacc ttggcaggcc gttcgaccac gctggcggtg ccgcccggta gagacgtgct
     1021 gcacgaggtg atgcgggtac gccccgacgt cccgtatgga tgtacgaacg gaatgtgtgg
     1081 cacctgcagg gcgaaggtgg tcgagggccg agtcgagatg gatcactgct acgcactgga
     1141 tcagggtgag ctcgacgccg gattcgtgtt gacctgccag gcgcagccgc gcagcgaggt
     1201 gctcgtgctc gactacgacg cgtaatccgt cagcacgatg tgcaggtcct cgagactgaa
     1261 ccaggccgcg cgtcgcagtg agccgcggaa ctcgatcagt gcctggccga ggtagcgacc
     1321 gtcctcgatc atcaccagct cgtcgaggat cttgcgcacc ggccacgacg ggttctccgg
     1381 gacgtcgcgg tagtcgatgc gcaacacgtc gacacccggc gtgaaagcgc tcggcccgat
     1441 cgaggtcagg aagcgaaacg cactgcatcc gcctgcctcg tcgatcgtcg cgtagttcgg
     1501 caacgtgacg cgcatcaggc gtcgggcgcc tgcggtgaaa aggttgcgac cctgttcggc
     1561 gtccgggtcg aaggtcttgc ccttccacgg cagccacatc gaggtcaacg cctcaaacgc
     1621 cgggtcgatg tggtggccga tcgtgctcgc caggaagcgc ccgcgcatcg ggccacgaag
     1681 cctcgcggcc ggctgaccgc cggcgaagac ggactcaagg gcggcgagcc cagttggtcg
     1741 ctcgaggcgg agcagcgcga ccgcccgcca gaagctcgtc gccgccaaga cttccgattc
     1801 catccggaca ttctgtatca cggcgccagg ttgcgacatc cttccggtgg aagtcccgag
     1861 ccgagcagaa caggagacca ccatggctga cggcgatttt gtccagctcg ccgaaacgat
     1921 ctcacccgga cgccgattgg gccgccatgt cgaacacgat gcccgcagcc gcaactttcc
     1981 ggccgcggtc gtcccgatcg cggacgtcac gcatgtccgc cacggcgagc ccttcgacca
     2041 gggccagctc ggttcctgca ccggcaacgc gatggcgggc gcgctcatga ccgcgccgta
     2101 ctaccagtcc ggccggacgc tggtcgaggc gagcgcggtg cagctgtatg agcaggcgac
     2161 tcatctcgac aatgtgcagg gcagttaccc ccccgacgac acagggtcga ccgggctggc
     2221 ggtggcgaag gcggccaagc gcgacggcta catcagcgcg tacagccacg ccttcgggat
//
LOCUS       DATCBJ010000622         2212 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_498156, whole genome shotgun sequence.
ACCESSION   DATCBJ010000622 DATCBJ010000000
VERSION     DATCBJ010000622.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2212)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2212)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2212
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_498156"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..641)
                     /locus_tag="VII50_12500"
     CDS             complement(<1..641)
                     /locus_tag="VII50_12500"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/oligopeptide/nickel ABC transporter
                     ATP-binding protein"
                     /protein_id="HEY5051713.1"
                     /translation="MTNDVTSATSHDASVSKPAHPALPPLLETRGMTKHFKIGGNFSR
                     KSLHAVDDVNIIINRQEIVALAGESGSGKSTIARLLARIYKPTSGEIYYQGKPLSSMR
                     TRRDSLGYRSDVPMVFQDPFSSLNPAYRVNHGIMRGLKLHRRDLTSREQRYDEAERVV
                     DAVGLTPASDILQRYPYELSGGQRQRIGFAQALAYKPKLILADEPVSMLDVSIR"
     gene            complement(638..1639)
                     /locus_tag="VII50_12505"
     CDS             complement(638..1639)
                     /locus_tag="VII50_12505"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY5051714.1"
                     /translation="MSSAPILEVSGLRVEYSTSRGAVCAVDNVDLEVRKGEFVGVVGE
                     SGCGKSTMLFAIAALLSPPAAVTAGTVKFHGHNLVTMTEKQLNVLRWRDYSVVMQSAM
                     NALNPVTTIAAQFKDVLKAHGAASNNEIANRSAEVMRLVGIDPIHLKSYPHQLSGGMR
                     QRSMIAMALLFTPDLVIMDEPTSALDVVAQRSLMVQIKELQHQLGFAVIFVTHDMSLV
                     SHFSDQLIVMYAGQVSEIGPTRQVFDHPAHPYSRGLLDAFPSIRGPRVELTGIPGSPP
                     DLARPPQGCRFHPRCPQVFDACRVTEPGLYEVGAVQARCLLHAAGGAPLLEASGATT"
     gene            complement(1617..>2212)
                     /locus_tag="VII50_12510"
     CDS             complement(1617..>2212)
                     /locus_tag="VII50_12510"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY5051715.1"
                     /translation="LIGVSAAYLGGIGDEALSLLTNVFLVIPTFPLIIVLATYAGKGN
                     LLVVIVVLVVTGWSYGANQLRAQTLSLRNRDFLESARVRGERRSYIIVAELLPTMTSL
                     IVANFLGAALYAVLTAAGLQFLGLGDPSSLSWGTMLYWSQNDGALQSGFPAWAIAPGI
                     CVALLGASFALLNYAFDEISNPALRPVRRIRVKRTHS"
BASE COUNT          421 a          701 c          713 g          377 t
ORIGIN      
        1 cggatcgaca cgtcgagcat cgagaccggc tcgtcggcca ggatcagctt cggtttgtac
       61 gccagcgcct gcgcgaaccc gattcgctgc cgctgcccgc cgctgagctc gtacgggtag
      121 cgctgcaaga tgtcggaggc cggcgtgagg ccgaccgcat cgacgacgcg ttcggcctcg
      181 tcgtagcgct gctcgcgcga cgtcagatcc cggcgatgca gtttcaggcc gcgcataatg
      241 ccgtggttca cccggtacgc ggggttcaat gagctgaagg gatcctggaa gaccatcggc
      301 acgtcgctgc ggtagccgag gctgtcacga cgggtgcgca tcgacgacaa cggcttgccc
      361 tgatagtaga tctcaccgct ggtgggcttg tagatacgag cgagcagccg ggcaatggtg
      421 ctcttgccac taccgctctc gccggcaagc gcgacgatct cctgccggtt gatgatgatg
      481 ttcacgtcat caacggcatg caaactcttg cgcgagaagt tgccgccaat cttgaaatgc
      541 ttggtcatgc cacgggtctc gagcagcggc ggcagggcag ggtgggccgg tttgctcacg
      601 ctcgcgtcgt ggctcgtggc gctggtcacg tcgttcgtca cgtcgtcgct ccggatgctt
      661 caaggagcgg cgccccaccg gcagcgtgca gcaagcaccg cgcctgcacg gcaccgacct
      721 cgtacagccc tggctcggtc acccgacacg catcgaacac ttgcgggcat cgagggtgga
      781 accgacaccc ctgcggcggc ctcgcgaggt ccggcgggct gccgggaatg ccggtgagtt
      841 cgacccgcgg tccgcgaatc gacgggaacg cgtcgagcag gccccggctg tagggatgag
      901 cagggtggtc gaaaacctgt cgggtcggcc cgatctcgga cacctggccc gcatacatga
      961 cgatcaactg gtcggagaag tggctcacca gtgacatgtc gtgagtcacg aagatgaccg
     1021 cgaaacccag ctgatgctgg agttctttga tctgcaccat gagcgagcgc tgtgcgacga
     1081 cgtcgagggc cgacgtcggc tcatccatga tgaccaggtc gggcgtgaag agcagcgcca
     1141 tcgcgatcat cgatcgctgc cgcatgccgc cgctgagctg gtgcggataa ctcttgagat
     1201 ggatgggatc gatcccgacc aaccgcatga cttcggccga gcggttggcg atctcgttgt
     1261 tcgacgctgc tccgtgcgcc ttcaggacat ccttgaattg cgcggcgatc gtcgtcaccg
     1321 ggttcagcgc gttcatcgcg ctctgcatga caacggagta gtcacgccac cgaagcacgt
     1381 tcaactgctt ttccgtcatc gtgacgaggt tgtgcccgtg gaacttcacg gtgcccgcgg
     1441 tgacggccgc cggcggactc agcagcgctg cgatcgcgaa gagcatcgtc gacttgccac
     1501 aaccggattc accgacaacg ccgacgaact cgcccttgcg gacttcgaga tcgacgttgt
     1561 cgacggcgca caccgctccg cgcgaggtgc tgtactcgac ccgcaggccg gagacctcaa
     1621 gaatgggtgc gcttgacacg tatcctccgg accggccgca gcgccgggtt gctgatctcg
     1681 tcgaatgcgt agttgagcag cgcgaacgat gcgccgagca aggcaacaca gatgcctggc
     1741 gcgatagccc atgccgggaa gccgctttgc agggccccgt cattttgcga ccaatacagc
     1801 atggtgcccc aactcaacga cgagggatcg ccaaggccaa ggaactgcaa accggccgcg
     1861 gtgagcaccg catagagcgc cgcgccaagg aagttcgcca cgatcagcga cgtcatcgta
     1921 ggcagcaact cggcgacgat gatgtacgac cgccgttcgc ccctcacccg cgccgattcg
     1981 aggaagtcgc ggttgcgtag cgacagcgtc tgcgcacgca gctggttggc accatacgac
     2041 cagccggtca ccaccaagac cacgatgacg accaacagat tgcctttgcc ggcataggtc
     2101 gcgagcacga tgatcagcgg gaacgtgggg atgacgagaa agacgttggt aagcaacgag
     2161 agtgcctcgt cgccgatgcc gccgaggtac gccgccgaca ctccgatcag ca
//
LOCUS       DATCBJ010000623         2386 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_498431, whole genome shotgun sequence.
ACCESSION   DATCBJ010000623 DATCBJ010000000
VERSION     DATCBJ010000623.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2386)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2386)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2386
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_498431"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..759)
                     /locus_tag="VII50_12515"
     CDS             complement(<1..759)
                     /locus_tag="VII50_12515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016337099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HEY5051716.1"
                     /translation="MTFDFKVADLGLAAFGRKEISLAENEMPGLMAMRAQHGETKPLT
                     GARITGSLHMTVQTAVLIETLVTLGAQVRWASCNIFSTQDHAAAAVAVGPTGTVDAPA
                     GVPVFAWKGETLEEYWWCTEQVLVWPEGDGPNMILDDGGDATLLVHKGVEFEKAGAVP
                     DPSTAESEEFEVVLRVLQRSLAEDPQRYTKIGQSIKGVTEETTTGVHRLYQMVETGEL
                     LFPAINVNDSVTKSKFDNLYGCRHSLIDGINRATD"
     gene            complement(827..1798)
                     /locus_tag="VII50_12520"
     CDS             complement(827..1798)
                     /locus_tag="VII50_12520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009083029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HEY5051717.1"
                     /translation="MTASGGSRAVVAALAANLGIAVTKFVAFAFTGSSSMLAEAVHSV
                     ADTGNQGLLLLGGRRARKERDNVHQFGYGSERYFYAFVVSLVLFSVGGVFAVYEGITK
                     IAHPHHLDNAIVAVVVLAVAVCLESYSFHTAMTESSKIKGDQSWVSFVRRTKNPELPV
                     VLLEDTAALFGLTFALAGVALSVLTHNPRWDGVGTLAIGALLVTVAVVLSIETKSLLI
                     GEAAAPEVQQRISEALMAGPEVESVIHMRTLHLAPDELLVAAKIAVAHDDTAATVARG
                     IDAAERRVRAAVPIAKIIYLEPDLQRPTVPPPASPAGDAGNTPDSDV"
     gene            complement(1876..>2386)
                     /locus_tag="VII50_12525"
     CDS             complement(1876..>2386)
                     /locus_tag="VII50_12525"
                     /inference="COORDINATES: protein motif:HMM:NF021916.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HEY5051718.1"
                     /translation="IAGALPVIWGAGALCRVAAKRFARQLHANAKYPALDGGLQEAAH
                     GTVALFDGMFATTTPEDVFRDPFDDESPRTMPLRVVLMRDSPESETETDAGLRRAAEH
                     LAGERGVRTSQVAADNGLPFERLAQLIALGDFASVYLALGLGIDPSPTRSVLEMKERI
                     ESSRRNREG"
BASE COUNT          418 a          813 c          747 g          408 t
ORIGIN      
        1 gtcggtggca cggtttatgc cgtcgatcag cgagtgacga cagccataga ggttgtcgaa
       61 cttggacttg gtcaccgagt cgttgacgtt gatggccggg aacaacagct cgccggtctc
      121 gaccatctgg tagagacggt gcacgcccgt ggtggtctcc tcggtgacac ctttgatgct
      181 ctggccgatc ttggtgtagc gctgtgggtc ttcagccagc gagcgttgca gcacccgcag
      241 cacgacctcg aactcttcgg actcagccgt cgacgggtcg ggcacggcgc cagccttttc
      301 gaattcaaca cccttgtgga caagcagagt cgcgtcgccg ccgtcgtcca aaatcatgtt
      361 cggaccgtca ccctcgggcc acaccaacac ttgctcggtg caccaccagt actcctcgag
      421 agtctcgccc ttccacgcga acaccggcac gccagccggt gcgtccacgg tgccagtcgg
      481 tccgaccgca actgcggcag ccgcgtgatc ttgagtcgag aagatgttgc aactcgccca
      541 tcgcacctgt gcacccagag tcacaagcgt ttcgatcagg acggcggttt gcacggtcat
      601 gtgcagcgag ccggtgattc gcgcgccggt gaggggcttg gtctcgccgt gctgagcgcg
      661 catcgccatc aggccgggca tctcgttctc ggcgagcgag atctctttgc gtccgaaggc
      721 ggccagccca aggtcggcaa ccttgaagtc gaacgtcatg cctgtgtctc ctcattcgtc
      781 ggcgctgtca ggttacgaga atactggcgc gcctccgcgc tcacgcctaa acgtccgaat
      841 cgggggtgtt gcctgcgtca cccgcgggac tggcgggggg aggcacggtc ggccgctgga
      901 gatcgggctc gagatagatg atcttggcga tcggtaccgc tgcccggacc cttcgctccg
      961 ccgcatcgat cccccgggca accgtcgctg ccgtgtcgtc gtgggccacc gcaatcttcg
     1021 cggcgacgag caactcgtct ggtgcgagat gcagcgtgcg catgtggatc accgactcga
     1081 cctcaggacc ggccatgagc gcctcgctga tgcgctgctg cacctccggc gcagcggctt
     1141 cgccgatcaa caggctcttt gtctcgatcg acagcacgac cgcaaccgtc accagcaagg
     1201 ccccgatcgc gagcgtcccg acgccgtccc atcgcggatt gtgcgtgagc accgacagcg
     1261 caacacccgc cagcgcgaac gtgagaccga acagtgccgc cgtgtcttcg agcaggacga
     1321 cgggcagctc gggattcttc gtccgacgca caaagctcac ccacgactgg tcgcccttga
     1381 tcttgctcga ctccgtcatc gctgtgtgga acgagtacga ctccaagcag accgcaacgg
     1441 cgagaaccac gacggcgacg atcgcgttgt cgaggtgatg cgggtgcgca atcttggtga
     1501 tgccctcgta caccgcgaag acaccaccga cgctgaacag caccagtgag acgacgaagg
     1561 cgtagaagta acgttccgag ccgtagccga actggtgcac gttgtcgcgc tctttgcgag
     1621 cgcgccgacc accgagcagg agcagtccct ggttgccggt gtcggcgacc gagtgcacgg
     1681 cctcggccag catcgacgac gacccggtga acgcgaaggc gacgaacttg gtcaccgcga
     1741 tcccgaggtt ggccgccagg gccgcaacga cggcacgaga gccgccggag gcggtcatac
     1801 ggcgctgccg ccggaggctg tcatacggcg ccgcccgacc cgcacgaggt gctcatgcgt
     1861 ggccgcctag cgtgctcatc cctcgcgatt tcggcggctc gactcgatcc gttccttcat
     1921 ctccagcacg gagcgcgtcg gcgagggatc gatgcccaac ccaagagcga gatagacgct
     1981 cgcgaagtcg ccgagcgcga tgagctgcgc cagccgttcg aacggaagcc cattgtccgc
     2041 ggccacctgg ctcgtgcgca caccccgctc tcccgcgagg tgttcggccg cccgccgaag
     2101 gccggcgtcg gtctcggtct cactctccgg agaatcgcgc atcagcacga ctcgcagtgg
     2161 catggtcctc ggcgattcgt cgtcgaacgg atcgcgaaac acatcctcgg gcgtcgtcgt
     2221 cgcgaacatc ccgtcgaaca acgcgaccgt accgtgcgct gcctcctgca acccgccgtc
     2281 caacgccggg tatttcgcgt tcgcatgtaa ctggcgggca aatcgcttgg ccgccacgcg
     2341 gcacagtgcg cccgcacccc agatcaccgg cagggcgccg gcgatg
//
LOCUS       DATCBJ010000624         2141 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_276983, whole genome shotgun sequence.
ACCESSION   DATCBJ010000624 DATCBJ010000000
VERSION     DATCBJ010000624.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2141)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2141)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2141
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_276983"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..848)
                     /locus_tag="VII50_12530"
     CDS             complement(<1..848)
                     /locus_tag="VII50_12530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011718902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate-binding protein"
                     /protein_id="HEY5051719.1"
                     /translation="MGAVGANEQAAGYRSPLLDLPGAVATDSWSSGTAAALDVDSGVA
                     AHYGDPLREQRQLANGAGLVDLSHRTVIRISGTDRLTWLHNLTTQGLERLAPGVGAQA
                     LILSPQGHVEHHLQLVDDGEAVWIHVERDDAQPLVDFLTSMRFMLRVEVDNLTADWAV
                     VWEPILEPHASLLSRVDPQRDEVAGRQVFVPRNGVLAFATAADRTGPPAGVLALEALR
                     VAAGRPRFGFETDHRTIPHELGWIGTAVQLDKGCYRGQETVARVANLGRPPRRLVRLH
                     LDGSAR"
     gene            complement(864..1016)
                     /locus_tag="VII50_12535"
     CDS             complement(864..1016)
                     /locus_tag="VII50_12535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5051720.1"
                     /translation="MPIFTFVSTQTEHMEDVPMQLLAEHLPISLLWDLADPAGPDSAD
                     LLAHEA"
     gene            1478..2041
                     /locus_tag="VII50_12540"
     CDS             1478..2041
                     /locus_tag="VII50_12540"
                     /inference="COORDINATES: protein motif:HMM:TIGR03083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03086 family metal-binding protein"
                     /protein_id="HEY5051721.1"
                     /translation="MADTAELDDVLHTTGAIVAGVKPDQYGLPTPCPDYDVGTLLNHI
                     VGWVGLFADGASQQPPQNDPATYQVGPHPAAEFDASAKRLVAAFHDGAAERDIDLGNG
                     PSPGSMILGVILMEYIGHGWDLAMATDQPVPYSERSIAAAKAAAQSNLKPEYRGPDKS
                     FGDIVEVPDDAPALDELIGLLGRDPRG"
BASE COUNT          377 a          696 c          708 g          360 t
ORIGIN      
        1 tcgcgcgcgc tgccgtcgag gtgaagccga acgagacggc gcggcggccg accaagattc
       61 gcaacacgcg ccaccgtctc ctggccgcgg taacaaccct tgtccagctg caccgccgtc
      121 ccgatccagc ccagctcgtg cgggatcgtg cggtggtcag tctcaaatcc gaaccgtggc
      181 cgcccggctg caacccgaag agcttcgaga gcgaggacgc cggcgggcgg gccggtgcga
      241 tcagcggcgg tcgcgaaggc cagaacgccg ttgcgaggca cgaaaacttg ccgccccgcg
      301 acctcgtcgc gctgcggatc gaccctgctc agcaagctcg cgtgcggctc gaggattggc
      361 tcccacacga cggcccagtc ggccgtcagg ttgtcgacct cgacccgcag catgaatcgc
      421 atcgaggtaa ggaaatcgac gagcggctgg gcgtcatccc gttccacgtg aatccagacc
      481 gcttcgccat cgtcgacgag ttggagatgg tgctcgacgt ggccctgcgg gctcagaatc
      541 agggcttgcg ccccgacgcc aggagccagc cgttcgaggc cttgcgtcgt gaggttgtgc
      601 aaccaggtca gccgatccgt gccgctgatc cggatcacgg tgcgatggga taaatcgacg
      661 agcccggcgc cattcgccaa ctgccgctgt tcgcggagtg ggtcgccgta gtgtgcggcc
      721 actccgctat cgacgtcgag tgccgccgcc gtgccactcg accagctgtc ggtggccacc
      781 gcgcctggca ggtcgagcag cgggctgcgg taacccgcgg cctgctcatt agcccctacc
      841 gcgcccatag ggcccagtct aagtcaggct tcgtgcgcga gcaggtcagc gctgtcgggg
      901 ccagcagggt cggcgagatc ccaaagcagc gagatgggca ggtgctccgc cagcagctgc
      961 atggggacgt cttccatgtg ttccgtttgt gtactcacga aagtaaagat cggcacccga
     1021 atggcgtcgc tgacccgccg atagggtgat agatcgcgcg caaatgccgc atttggctat
     1081 ttggccctaa ccgagtacca accctacgta ccgagctcat gcgcaggtcc acgcccccga
     1141 atgtgattgc cgagcgttcg cgccgtgatt tacgttgggg gtacacgaga gaggaggtgg
     1201 tctggagaat gtgtagtagc cggacgcgtg aggtggctgt cagctagccg ccgtcctatt
     1261 cggacgtgcc tctgagatcc gaggttgacg tcgcctggat ggcgatggcg aaaccacgac
     1321 agctcatccg acccccggac tgccggacat cgtccagccg gcccgtgtcg cgcgaacctg
     1381 tgcggcacgg cgcagtccgg gggtcgaaca ctgtgtttat cgagcactgg ttctggagca
     1441 ctgtgtttac ggcccgcaat cggggcaaga tcgggccatg gccgataccg ctgaactcga
     1501 tgacgtcctg cacacgaccg gcgcaatcgt cgcgggtgtg aagcccgatc aatacgggtt
     1561 gccgacaccg tgcccggact acgacgtcgg gacgctgctc aaccacatcg ttggctgggt
     1621 cgggcttttc gccgacggcg ccagtcaaca accgccgcag aatgatccag cgacttacca
     1681 ggttggcccg catccggccg ccgagtttga cgcgtccgcg aaacgactcg tcgccgcgtt
     1741 ccacgatggt gccgccgagc gggacatcga cttgggtaac gggccgtcgc cgggctcgat
     1801 gatcctcggc gtgatcctga tggagtacat cgggcatggg tgggatctcg cgatggccac
     1861 cgaccagccg gtgccgtatt cggagcggtc gatcgcagcc gcgaaagccg cggcacaatc
     1921 gaatctgaag cccgagtacc ggggtccgga caagtcgttc ggcgacatcg tcgaggtccc
     1981 cgacgacgcg cccgccctcg acgaactgat cgggttgctg ggccgcgatc cgcgcggcta
     2041 gtcagatctt cttcagccgc gcggacagat gcgggccaag cagctgaccg actgctgcca
     2101 tgtcgaacgc gtagagcagg tcgccgtcga ccgtcccgta g
//
LOCUS       DATCBJ010000625         2757 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_547320, whole genome shotgun sequence.
ACCESSION   DATCBJ010000625 DATCBJ010000000
VERSION     DATCBJ010000625.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2757)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2757)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2757
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_547320"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..422)
                     /locus_tag="VII50_12545"
     CDS             complement(<1..422)
                     /locus_tag="VII50_12545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011718871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-containing protein"
                     /protein_id="HEY5051722.1"
                     /translation="MASLKQFRLPDVGEGLTEAEIVHWHVKVGDVVTQNQTIVDIETA
                     KALVELPSPFAGVVSELLIPEGTTVEVGTPIIIIDVAPGAETLMAAGPVPVAQGESPA
                     VDDLVPSIPAVSEMSAGRTANLVGYGPRADSPVRRRQR"
     gene            complement(457..1440)
                     /locus_tag="VII50_12550"
     CDS             complement(457..1440)
                     /locus_tag="VII50_12550"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011718872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HEY5051723.1"
                     /translation="MAPVAIARALNMGLRKALESDPKVIIMGEDVGKLGGVFRVTDGL
                     QKDFGDDRVMDTPLAESGIIGTAIGMAMRGYRPICEIQFDGFVFPAYDQIVSQLAKLH
                     YRSQGLVPMPMVIRIPFGGGIGAVEHHSESPEALFAHVAGLKVVACSNSADAYTMIQQ
                     AVISNDPIIFFEPKRRYWEKAELDVDTPLAQAVPLHAARVVRPGRHLTLLAYGPMVKT
                     CLDAAKVAADEGHELEVVDVRSLSPIDWPTITDSVRHTGRCVVVHEAQKFLGMGAELA
                     ARITELCFYELQAPVLRVGGYPIPYPPSRLEEHFLPDLDRVLDTVDRSLSF"
     gene            complement(1440..2543)
                     /gene="pdhA"
                     /locus_tag="VII50_12555"
     CDS             complement(1440..2543)
                     /gene="pdhA"
                     /locus_tag="VII50_12555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011718873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045250 - cytosolic pyruvate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0004739 - pyruvate dehydrogenase
                     (acetyl-transferring) activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HEY5051724.1"
                     /translation="MTADHGALTSERDLVQLLTPEGERLEHPDYPLEISQAELRSLYR
                     DLALVRRFDTEATALQRQGELGIWASLLGQEAAQIGSGRALLPGDMAFPTYREHGVAW
                     CRGIGPENLLQLFRGVNFGGWDPKEHNFALYTIVIGAQTLHATGYAMGMQRDGSEDAV
                     IAYFGDGASSEGDVNEAFIFAASYNAPVVFFLQNNQWAISVPEERQSRIPLFRRAHGF
                     GFPGVRVDGNDVLACLAVTRKALQYAREGQGPFLVEAFTYRMGAHTTSDDPTRYRLAS
                     ELENWKLRDPIERVKAYLSRNGLADAAFYESIEAEGAELAARVRTACLTMPEPKPSFI
                     FEQVYAGGSPLVDEERAAFDEYMAGYESAEVGH"
BASE COUNT          497 a          878 c          889 g          493 t
ORIGIN      
        1 tgccgttggc gacggcggac tggggagtcg gcgcgcggac cgtaaccgac gaggttggcg
       61 gtgcgtcctg cactcatctc agagaccgcg ggaatcgagg ggaccaggtc gtccactgct
      121 ggtgactcgc cctgcgctac cggaacgggc cccgcagcca tcaacgtctc cgcaccgggt
      181 gcaacgtcga tgatgatgat cggcgtcccg acctcgacag ttgtcccttc ggggatcagc
      241 aactcgctga ccacaccggc gaatgggctt ggtaattcga ccagtgcctt cgcggtttcg
      301 atgtcgacga tggtctggtt ctgcgtcacc acgtcaccga ccttgacgtg ccagtgcacg
      361 atctcagctt cggtcagacc ctcgccgaca tcggggagtc tgaactgctt gagcgatgcc
      421 acatcgcacc tccgtgcgtg gtcagtacgg cgctgttcag aacgacagtg atcggtcgac
      481 agtgtcgaga acgcgatcga gatccggcag gaaatgctcc tcaagacgcg atggcgggta
      541 cgggatcggg tagccgccga cccgcagtac cggtgcctgc aactcgtaga agcaaagttc
      601 agtgatacga gcggccaact cggcgcccat gcccaagaat ttctgtgctt cgtgaacgac
      661 gacgcagcgc ccagtgtgcc gcacggagtc ggtgatcgtc ggccagtcga tcggcgatag
      721 cgaccggacg tcgacaacct cgagttcgtg accctcgtct gccgccacct tcgcggcgtc
      781 gaggcaggtc ttcaccatcg gtccgtacgc gagcaacgtc agatgccgac ccggccggac
      841 gacgcgcgcg gcgtgcaacg ggacagcttg cgcgagcggg gtgtcgacgt cgagctctgc
      901 cttttcccag taacgccgct tcggctcgaa gaagatgatc ggatcgttgc tgatgacggc
      961 ctgttgaatc atcgtgtagg cgtcagcgga gttggaacac gcgacgacct tgagacccgc
     1021 cacgtgggcg aacagcgcct cgggggattc gctgtgatgc tcgacagcac cgatgccgcc
     1081 gccgaacggg atgcggatga ccatcggcat cggcaccagg ccctgcgatc ggtagtgcag
     1141 tttcgcgagt tggctgacga tctggtcgta ggcggggaag acgaagccgt cgaactggat
     1201 ctcgcagatg ggtcggtagc cgcgcatcgc catcccgatc gcggtgccga tgatgccgga
     1261 ctccgcaagc ggcgtatcca tgacacggtc gtcgccaaag tccttctgca aaccgtcggt
     1321 cacccggaac acgccgccga gcttgccgac gtcctcgccc atgatgatga ccttggggtc
     1381 tgattccagg gccttgcgca ggcccatgtt caacgctcgc gcaatcgcta ccggcgccat
     1441 cagtgaccca cctcggccga ctcgtacccc gccatgtact cgtcgaacgc cgcgcgctct
     1501 tcgtccacta gcggcgaacc tccggcgtag acctgctcga agatgaagct gggcttgggc
     1561 tccggcatcg tcaagcacgc ggtcctgact cgggctgcga gctcggcacc ctcggcttcg
     1621 atcgactcgt agaaggcagc gtccgcgagc ccgttgcgcg acaagtaagc cttcacgcgc
     1681 tcgatgggat cacgcagctt ccagttctcg agctcgctcg cgaggcggta gcgggtcggg
     1741 tcgtccgacg tcgtgtgcgc gcccatgcgg taggtgaacg cctcaacgag aaacggtccc
     1801 tggccctcgc gtgcgtactg caaagccttt cgcgtcacag caaggcaagc gagcacgtcg
     1861 ttgccgtcga cgcggacgcc ggggaagccg aagccatgtg cgcggcggaa cagcggaatg
     1921 cgcgactggc gttcttcggg gaccgagatc gcccactggt tgttctgcag aaagaacacc
     1981 accggagcgt tgtaggacgc cgcaaagatg aacgcctcgt tgacgtcgcc ctcactcgat
     2041 gcgccgtcgc cgaagtaagc gatgacggca tcttcggagc cgtcgcgctg cattcccatc
     2101 gcgtagccgg tggcgtgcag cgtctgcgct ccgatgacga tcgtgtacag cgcgaagttg
     2161 tgctccttcg ggtcccagcc gccgaagttc acgccgcgaa aaagctgcag caggttctcc
     2221 ggtccgatac cgcggcacca ggcaacgccg tgctcgcggt aggtcggaaa cgccatgtca
     2281 cctgggagca gagctcggcc ggagccgatc tgcgcagcct cttggccaag cagcgacgcc
     2341 cagatgccca gctcgccctg ccgctgaagc gcagtcgcct cggtatcgaa gcgccgtacg
     2401 agcgcgaggt cgcgatagag gcttcgcagc tcggcttgcg agatttccaa cggatagtcg
     2461 ggatgctcga gccgctcccc ctcgggagtc agcagctgga caagatcgcg ttcactcgtt
     2521 agtgcgccgt ggtcggccgt catgcgtgcc actcctcctg ctcgccaacg cgaggatcac
     2581 tcgtcgatgg gcgccggacc gccttgccgg taggcaacat cggaccgggt acctaggcaa
     2641 tcgttgcaga tcgaaggcca gtagttggca tccgtggaag ccctagctgt tgcgtggaag
     2701 atctagcagt tatcgatcca gctttaacgg tcggggtggt gcgggcacgg gtgtatc
//
LOCUS       DATCBJ010000626         2942 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_84653, whole genome shotgun sequence.
ACCESSION   DATCBJ010000626 DATCBJ010000000
VERSION     DATCBJ010000626.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2942)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2942)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2942
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_84653"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..636
                     /locus_tag="VII50_12560"
     CDS             <1..636
                     /locus_tag="VII50_12560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5051725.1"
                     /translation="PEHLEVFMIRRHMGMLFAGGMYVFPGGVVDATDHVGRDHREAHA
                     FAAIRETYEECGVLFASGPETDLANLEADRLALIEHRATFDEVLARHAAMAQPHLLQP
                     WSRWITPDFEPRRYDTYFYVAIAAAQHKDARFVGGEADAAQWVAPEHAIAAQERGEWL
                     LMPPTEWTLRELATFTAAADVFAAGVGRNLAPVQVRIDLDTEPARWVVLEP"
     gene            633..1448
                     /locus_tag="VII50_12565"
     CDS             633..1448
                     /locus_tag="VII50_12565"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEY5051726.1"
                     /translation="MTTTASPEHATARAFLVRAGNRSPMTLEGTNTWVLVEPGQSQAV
                     VIDPGPDDARHRDAIMVAIETAGANQVALIVLTHGHADHAEGAPSLAQRVRAPTRSIA
                     PAHCSPGQQPLSDGERLRIGGLDLDVIATPGHTADSACFVIDADDALLTGDTILGTGS
                     TVVARPDGGLADYLATLHRLRALVDERQLRAVLPGHGPVVNDPAAKLDAYSQHREERL
                     DQVRAAASSAATVDQVLHAVYGDVVSDLQFAARWSLLAQLDYLRDQGFNVPAE"
     gene            complement(1429..2103)
                     /locus_tag="VII50_12570"
     CDS             complement(1429..2103)
                     /locus_tag="VII50_12570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003975365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEY5051727.1"
                     /translation="MDDVLGKLPLFAALDDDANVALRVAMRHEHFSRGDVIFDEGDPG
                     TKLFAVIEGKVKLARTAPDGRENLQAVLGPGEMFGELSLFDPKPRTAGAVALTDTVLA
                     SLAHEELRRWITGRPDVAVQLLQALAQRLRRTNDVLADLVFSDVPSRVAKALLGLAER
                     FGQPTTEGIHVAHDLTQEELAQLVGASRETVNKALADFSARGWIRIESRAVVIRDVEG
                     LTRRAR"
     gene            complement(2170..2778)
                     /locus_tag="VII50_12575"
     CDS             complement(2170..2778)
                     /locus_tag="VII50_12575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5051728.1"
                     /translation="MRLSSHKHRINRVLESQWDGVALSAGSSQSGTRRAPERSTDFDH
                     LSIGDLRNYRTALAEEENRVSYWRRIIQARLDVVHAGETGAARVDNLRNVLADAHLTS
                     SRRALVTIMPIEDVPPLPDLATLWEREPRAADPAYNEVLARDLSNAEAQLSAYRNALH
                     RRISGSTDELIARYREDPVLCLLALPADQKEAASRRALAERR"
BASE COUNT          474 a          968 c         1015 g          485 t
ORIGIN      
        1 ccggaacatc tcgaggtctt catgatcagg cgccacatgg ggatgctctt tgccggcggc
       61 atgtacgtct ttccgggcgg cgtggtcgac gccaccgacc atgtcggccg cgatcatcgg
      121 gaggcccacg cgttcgcggc catcagggag acatacgagg agtgcggcgt cctgttcgcg
      181 tcgggtccgg agaccgacct cgcgaacctc gaggccgacc ggctcgcgct gatcgagcat
      241 cgtgcaacgt tcgatgaagt cctggcgcgg cacgcggcga tggcgcagcc acacctcttg
      301 cagccatggt cacgctggat cacccccgac ttcgaaccgc gccgctacga cacgtacttc
      361 tatgtcgcca tcgcagccgc gcagcacaaa gacgcgcgct tcgtcggcgg ggaggccgac
      421 gcggcgcagt gggtcgcgcc ggaacacgcg atagcggcgc aagaaagggg cgagtggctg
      481 ctcatgccac ccaccgagtg gacgcttcgt gagctcgcga cgttcacggc ggcggcggat
      541 gtcttcgccg ctggtgtcgg ccgcaacctc gcgccagtgc aggtgcgtat cgacctcgac
      601 accgagccgg cgcgctgggt ggtactcgaa ccgtgacaac caccgcgagc ccagagcacg
      661 cgaccgcgcg ggccttcttg gtgcgcgccg gcaaccgctc tccgatgacg ctcgagggca
      721 ccaacacctg ggtgctcgtg gagccgggcc aaagccaggc cgtcgtcatc gatccgggtc
      781 cggacgacgc gaggcaccgc gacgcgatca tggtggcgat cgaaacggct ggcgccaacc
      841 aggtcgcgct catcgtgctg actcacggtc acgccgacca cgctgagggc gcgccgtccc
      901 tcgcccagcg agtgagggcc ccaacccgct cgatcgcacc agcgcactgt tcgcccggtc
      961 aacaaccact gtcggacggc gagcggctac ggatcggcgg cttggacctc gacgtcatcg
     1021 cgacgccggg ccacaccgcc gactcggcct gtttcgtgat cgacgccgat gacgcactgc
     1081 tgaccggcga cacgattctc ggcacgggca gtacggtggt cgcgcggcct gatgggggtc
     1141 tggcggacta cctcgccacc ttgcaccgac tgcgggcact ggtcgatgaa cggcagctgc
     1201 gcgcggtcct gcctgggcat ggtcctgttg tgaacgaccc cgcggccaag ctcgacgcgt
     1261 actcgcagca tcgcgaagag cgcctcgatc aagtgcgggc ggcagctagc tcggccgcga
     1321 ctgtcgacca ggtgttgcac gcggtgtacg gcgacgtcgt gtcagacctg cagtttgcgg
     1381 ctcggtggtc actgctggcc cagctcgact acctgcgcga tcaaggcttc aacgtgcccg
     1441 ccgagtgagc ccttcgacgt cgcggatcac gaccgcgcgc gactcgatgc ggatccagcc
     1501 acgcgcgctg aagtcggcaa gcgccttgtt cacggtctcg cgcgaggcgc cgacgagttg
     1561 cgcgagctct tcttgcgtga ggtcgtgcgc gacgtggatg ccttcggtcg ttggctgacc
     1621 gaaacgttcg gcaagtccga gcaaggcctt cgccactcgt gacggcacat cgctgaaaac
     1681 caggtcggcc aaaacgtcgt tggtgcggcg cagccgttgc gccaaagctt gcaggagttg
     1741 gacggcgacg tcaggccgtc cggtgatcca gcgccgcagt tcctcatggg caagcgaggc
     1801 gagcactgtg tcggtcaacg ccacggcacc ggcagttcgc ggtttcggat cgaacagcga
     1861 cagttcgccg aacatctcgc cgggccccag gactgcctgc aaattctccc tgccgtcggg
     1921 cgccgtgcgg gcgagcttca ccttgccctc gatgaccgcg aacagcttcg tgcccgggtc
     1981 cccttcatcg aagatgacat cgccccgtga gaagtgctcg tgccgcatcg cgacgcgtag
     2041 ggccacgttg gcgtcatcgt cgagtgcggc gaacagggga agcttgccca acacgtcatc
     2101 cacgtcggtg attctcacac ttttgcaagc cgaaggcgcg aagtgtttgc gagccgaagg
     2161 cgcgaagtgc tagcgacgtt cggcgagtgc gcgccggctc gcggcttcct tctggtcggc
     2221 cggaagggcg agcaggcaca ggaccggatc ctcgcgatac cgcgcaatga gttcgtcggt
     2281 cgaaccggag atgcggcggt gcaaggcgtt gcggtacgcc gacagctggg cctcggcgtt
     2341 ggacaggtcg cgcgcgagga cctcgttgta ggcggggtcg gccgctcgtg gctcacgctc
     2401 ccagagcgtg gcaaggtcgg gcagcggcgg gacgtcttcg atcggcatga tggtgaccag
     2461 cgcccgacgg ctgctggtga ggtgggcgtc ggcgagtacg ttgcgcaggt tgtcgacacg
     2521 cgctgcgccg gtctcgccag catgaacgac atcgagcctg gcttggatga tgcgccgcca
     2581 gtaggagacc cggttctcct cctcggcaag tgccgtgcgg tagttgcgta ggtcaccgat
     2641 agagaggtga tcgaagtcgg tactgcgctc aggagcgcgg cgagtgcctg actggctgct
     2701 cccggcgctc aaagcgaccc cgtcccactg cgactcgaga actcgattga tgcggtgctt
     2761 gtggctactg aggcgcatcg gccggtccgg cgcgagcctt gaggcctagc gggctggggt
     2821 cacccgggtg gccgttcagg tcacccgtcc aggcgaatga caccctccgt gattaggctc
     2881 gacgtcgtgg cgcggcgggt gatcgaccag gaatcgccgc tcgcgcgtgt ccggcgggcc
     2941 cg
//
LOCUS       DATCBJ010000627         4686 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_167239, whole genome shotgun sequence.
ACCESSION   DATCBJ010000627 DATCBJ010000000
VERSION     DATCBJ010000627.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 4686)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4686)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4686
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_167239"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..707
                     /locus_tag="VII50_12580"
     CDS             <1..707
                     /locus_tag="VII50_12580"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FCD domain-containing protein"
                     /protein_id="HEY5051729.1"
                     /translation="PGDSLPPELPLSQQFGVSRTVLRESVKRLEEKGLVKVVQGRGTV
                     VQPTSAWTMMDRVVLTSLIAHDKTLGVLDELSIVRARLEAAMAGEAARLHTPTELEVL
                     REILHTMRQTVDDPVDFHAADVSFHETVMSISGNRLAESIARVLYERARDSARYLGAV
                     SPTLMHQTLLEHQAIFDAIEAGDADLSERVMDTHISDAWQRRRPPDHRGTKSRRKPVG
                     AERTTAVAPRSRIRPT"
     gene            954..2075
                     /locus_tag="VII50_12585"
     CDS             954..2075
                     /locus_tag="VII50_12585"
                     /inference="COORDINATES: protein motif:HMM:NF015155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HEY5051730.1"
                     /translation="MRRLAEFVLGHRRWVLLFWGVVLVAGIALAGKTTGRLTVDFSLP
                     GQPGTVTAQKIKADLGNGGDTDPYLVSVTLPAGQQVSGNTAAVAKVFSTVEQGVPNVR
                     VLDEATTGDRAFRTSDGKTAYALLFYRFNPSPTAKLQTDAIRAAATSAAPAGATIGVT
                     GEDALAVGSSSGGPGVLGETLLGALGALIVLAFVFASFLALLPLAVAAVSILATFLML
                     LPITYLTNVSFIVEFLIALIGLGVAIDYSLLLVTRWREERDHGRDNHDAVVVAMQTAG
                     KAVVFSGVTVAIGLLALVVLPVPFMRSLGYGGALIPLASVLTTLTLTPAILGGIGPRV
                     DWRGSGTRIGPASSGVAGPRSLCVAGGSPQALRWSRCSR"
     gene            2054..3100
                     /locus_tag="VII50_12590"
     CDS             2054..3100
                     /locus_tag="VII50_12590"
                     /inference="COORDINATES: protein motif:HMM:NF015155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HEY5051731.1"
                     /translation="MVALFALISVFFGIKIGLASSKSLAKTGPAYTALQTLENGGVTT
                     GTLTPMEILVSSDQARSTAARVAAISGVSHVFISTDPSSTRNGQTVIVAVPNTETVNS
                     HSVGVVKRVKSAVDGLPGVVGVAGVGAAQIDFLHAVYGNFPLMLLIIAVLTYLLLARA
                     FRSLLLPLKAVVLNLVSLGATLGLMVLFWQDGHGSNAIFGIAATGAVTFWIPLMVFAF
                     LFGLSMDYEVFILSRIREEYDAAKSTSAAVIEGVGRTGRLVTSAALILFLAFAALASG
                     PGTDLKTLATGLGFGILLDATIVRAVLVPSLVSLFGSWNWYLPATVAKILRVQPSYPH
                     PEHGEPRTPIAADA"
     gene            complement(3133..3723)
                     /locus_tag="VII50_12595"
     CDS             complement(3133..3723)
                     /locus_tag="VII50_12595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5051732.1"
                     /translation="MKTTADALVDRYLAELDHAMARLSVTKRHQILAEVSSHISEGRA
                     SLDTDDPASVRALLDRVGDPDAIATEAGATRAPARRSDAWVPWLLLFGGFAAAVGWFV
                     GVGLLWSSHTWRTRDKLIGTLILPGGLLGLFILGTHPSSGASCSSTTASDGQSVTHCV
                     THGFSFPWPLGIVVLVVALVAPIATSVHLQAVRRRA"
     gene            complement(3720..4070)
                     /locus_tag="VII50_12600"
     CDS             complement(3720..4070)
                     /locus_tag="VII50_12600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013350246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEY5051733.1"
                     /translation="MVPSDDPVWLAQLRRGSVEHCVLALLRDGERYGFELARILADAG
                     GLIASEGTIYPLLARLRRNGLVKTTWRESAEGPPRRYYRLTNAGERAVAAFVQNWKVF
                     RSAVDRILDETGAT"
     gene            complement(4144..>4686)
                     /locus_tag="VII50_12605"
     CDS             complement(4144..>4686)
                     /locus_tag="VII50_12605"
                     /inference="COORDINATES: protein motif:HMM:NF024091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEY5051734.1"
                     /translation="GTGMIRTTLTAMPNRVAVLAAKAIVVAGWALVAGIVGVLGSTLL
                     GQFMLPGKGFTPANGYMSLALSDAGMLRAAFGSVLYLVLIALLSLGIATAVRDSAAGI
                     GVVLGLLFAFPIALVMVSNPHWHRHLEQIAPMNAGMEIQATIDLHGLPLSPWAGLGVL
                     GLWAAGALILGALVLQLRDA"
BASE COUNT          770 a         1521 c         1575 g          820 t
ORIGIN      
        1 cgccaggcga ttcgctgccg ccggagctgc cgctgagcca acagttcggc gttagcagga
       61 ctgtcctccg ggagtccgtc aagcgcctcg aagaaaaggg tttggtcaag gttgttcagg
      121 gccggggaac ggtcgttcag ccgacgagtg cctggaccat gatggatcgc gttgtgctga
      181 cgtcgctcat cgcccacgac aagacgttgg gcgtgctcga cgaactgagc attgtccggg
      241 cacgtctcga ggccgcgatg gccggcgagg ccgcgagact acataccccg accgaactag
      301 aggtgctgcg agagatcctt cacacgatgc gccagacggt cgacgatcct gtggatttcc
      361 acgcggccga tgtctcattt cacgagacgg tgatgtcaat ctcgggcaac cgtctggcgg
      421 aaagcatcgc ccgcgtgctt tacgaacggg cgcgagacag tgcgcgctat ctcggggcgg
      481 tatcgccgac acttatgcac caaacgctcc tcgaacacca ggcgatcttc gatgcgatcg
      541 aggctgggga cgccgacctt tcggagcgtg tcatggacac gcacatcagc gacgcgtggc
      601 agcgccgtcg gccgcccgat caccgcggaa cgaagtctcg ccgaaagccc gtcggcgctg
      661 aacggacaac agcggtcgct ccccgtagcc gtatccgacc gacgtagcgg aacatgggtc
      721 gacacagacc caagcgtcgt cggctacggc tgtttgttgt ctgcgtggag tggtgcattc
      781 tgggtctcaa ggcgcaggac cagggcgatg ccggcgaggt cgttgttccg agcccgccgg
      841 ggtagggccc gatcgagcac gaatcgagaa gcgccgctga ccgcgcctgg catagcgtgg
      901 tcgcgatcgg ccccgattgc ggccatgagt gccagagcgc ggaggagacg gctgtgcgac
      961 ggttggctga gttcgtgctg gggcaccgcc ggtgggtgct gttgttctgg ggcgtcgtgc
     1021 tggttgccgg tatcgcgtta gccggtaaga cgaccggccg gctgacggtc gatttctcgc
     1081 tgcccgggca gccggggacg gtgaccgcgc agaagatcaa ggccgacctt ggtaacggcg
     1141 gtgacaccga cccatatctg gtctcggtga ccctgccggc cgggcagcaa gtctccggca
     1201 acaccgcggc ggtcgcgaag gttttcagca ccgtcgagca gggcgtgccg aacgttcggg
     1261 tgctcgacga ggccaccacc ggcgaccggg cgttccggac ctccgacggg aagactgcat
     1321 atgcgctctt gttctaccgg ttcaatccgt cgccgacggc caagctgcag accgatgcga
     1381 tccgcgccgc cgccacctcc gcggcacccg ccggtgccac gatcggggtc accggcgagg
     1441 acgcgctcgc ggtcgggagc tccagtggcg gccccggggt gctcggggag acgctgctcg
     1501 gcgcgctcgg cgccctgatc gtgttggcgt tcgtcttcgc gtcgttcctg gcgctacttc
     1561 cgctcgccgt ggcggcggtg tcgatccttg cgacgttctt gatgctgctc ccgatcacct
     1621 atctcacgaa tgtgtcgttc atcgtggagt tcctgatcgc cttgatcggt cttggggtgg
     1681 cgatcgacta ctcgctgctg ctggtcacgc ggtggcggga ggaacgcgac cacggccgcg
     1741 acaaccacga cgcggtcgtc gtggcgatgc agaccgcggg caaggcagtg gtgttcagcg
     1801 gcgtcacggt cgccatcggc ttgctggcgc tggtggtgtt gccggtgccg ttcatgcgca
     1861 gcctcgggta cggcggcgcg ctgataccgc tggctagcgt gctgaccacg ctgacgttga
     1921 cgccggcgat cctgggcggc atcggcccac gggtcgattg gcgcggatcc ggcacgagaa
     1981 tcgggccagc aagttctggg gtcgctgggc cgcgctcgtt gtgcgtcgcc ggtggatcgc
     2041 cgcaggcgct gcgttggtcg cgttgttcgc gctgatcagc gtgttcttcg gcatcaagat
     2101 cggcttggcg tcttcgaagt cgctggccaa gaccggcccc gcctacacgg cgctgcagac
     2161 cctggagaac ggtggtgtca ccaccggcac gctgacgccg atggagatcc tggtcagctc
     2221 cgaccaggcc cgcagcaccg ccgcccgggt cgctgcgatc tctggcgtca gccacgtgtt
     2281 catttctacc gacccgagca gcacccgcaa cgggcagacc gtgatcgtcg ccgtgccgaa
     2341 caccgaaacc gtgaactccc acagcgtggg cgtggtcaaa cgggtcaagt ccgcggtcga
     2401 tggcctgcca ggggtggttg gggtagccgg ggtcggggcg gcgcagatcg acttcctgca
     2461 cgcggtctac ggaaacttcc cgctgatgct gctcatcatc gcggtcctga cctatctgtt
     2521 gctggcgcgc gcgttccggt cgctgctgct cccgctgaaa gcggtcgtgc tcaaccttgt
     2581 ctccttgggg gcgacactgg ggttgatggt gctgttctgg caagacggcc acggctccaa
     2641 cgccatcttc ggcatcgccg ccaccggtgc ggtgacgttc tggatcccgc tgatggtttt
     2701 cgcgttcctg ttcgggctgt cgatggacta cgaggtgttc atcctgtccc ggatacgcga
     2761 ggaatacgac gcggccaagt ccaccagcgc cgcggtcatc gaaggcgtcg ggcggaccgg
     2821 ccgccttgtg accagcgccg ccctgatctt gttcctcgcc ttcgctgctc tcgcctccgg
     2881 tcccgggacc gacctgaaaa ccctggctac cggcctcggc ttcggcatcc tgctcgacgc
     2941 caccatcgtc cgcgccgtac ttgtgccttc actggtctcc ctgttcggat cctggaactg
     3001 gtacctgccc gcaaccgtcg cgaaaatcct gcgcgtgcag ccgtcctacc cccaccccga
     3061 acacggcgaa ccgcgcaccc ccatcgccgc cgacgcctaa ccgcgcagcc accaagctcc
     3121 aagcacgtgt ggtcatgccc gccgtcggac tgcttgaagg tggacggagg tcgcgatcgg
     3181 tgcgacgaga gccacgacga ggacgacgat gccgagcggc cacgggaagc tgaagccgtg
     3241 cgtcacgcaa tgcgtgacgc tttgtccgtc acttgcggta gtggacgagc acgaggctcc
     3301 agagcttggg tgcgtaccaa gaatgaacaa gccgaggagg ccgccgggaa gtatcaacgt
     3361 gccgatgagc ttgtctcgtg ttcgccaggt atgagacgac cacaacaagc cgactccgac
     3421 gaaccaaccg accgcggcgg cgaagccacc gaagagcaac agccacggca cccacgcgtc
     3481 agatcgtcgc gccggcgctc gggtggcgcc ggcttccgtg gcgattgcgt cggggtcgcc
     3541 gacccggtcg agcagtgcgc ggacgctggc tgggtcgtcc gtgtccagac tcgctctgcc
     3601 ttcgctgatg tggctggaca cttccgcgag aatctggtgg cgcttggtca ccgacaagcg
     3661 cgccatcgca tggtcaagtt ccgccaagta ccggtctacc agcgcgtctg cggtcgtttt
     3721 cacgttgcac ctgtctcatc gaggatccga tcgactgcgg accgaaacac cttccagttt
     3781 tggacgaacg cggcgacggc gcgctcgcct gcgttggtga gccggtagta cctgcgggga
     3841 ggtccctcgg cggattctcg ccaggtcgtc ttgaccagtc cgttgcgtcg caggcgagcg
     3901 agcagcgggt agatggtgcc ctcgctggcg atcagcccgc cggcgtcggc gaggatgcgg
     3961 gcgagctcga agccgtaccg ctcgccgtcg cgcagcaagg cgagaacgca atgctcaacc
     4021 gacccacgcc gcagctgggc aagccagacg gggtcgtcac ttggtaccat gcatcgcatg
     4081 gtagctggcc aacgcggttt ctactagctc gctaagcctt cgaagtgtcc gtcccgtcgg
     4141 cgctcaggcg tcacgcagct gcagcacgag cgcaccgaga atcagtgcgc cggcagccca
     4201 tagcccgagc acaccgagtc cggcccacgg gctgagtggc aggccatgca ggtcgatcgt
     4261 tgcttgaatt tccatgccgg cgttcatcgg cgcgatctgt tcgaggtggc ggtgccagtg
     4321 cgggttgctg accatgacaa gggcgatagg aaaggcgaat agcagcccga gcacaacgcc
     4381 gatgcccgcg gccgagtcgc gtaccgcggt cgcaatgccc aggctgagca gggcaatgag
     4441 cacgagatag agcaccgagc cgaacgcggc ccgcagcatc ccggcgtccg acaacgcaag
     4501 cgacatgtag ccgttggcgg gggtaaaccc cttgccaggc agcatgaatt ggccgagcag
     4561 tgtcgagcca aggacgccaa ctatgccggc aacgagcgcc caaccggcga cgacgatcgc
     4621 cttcgccgcg agcacggcaa cgcggttagg catcgccgtg agcgtggtgc gaatcattcc
     4681 ggtgcc
//
LOCUS       DATCBJ010000628         2894 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_387550, whole genome shotgun sequence.
ACCESSION   DATCBJ010000628 DATCBJ010000000
VERSION     DATCBJ010000628.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2894)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2894)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2894
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_387550"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..490
                     /locus_tag="VII50_12610"
     CDS             <1..490
                     /locus_tag="VII50_12610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HEY5051735.1"
                     /translation="ECDAANLAHPRLSVEPGRAICGPTTFTLYEVGTVKPVDVGAGVV
                     RTYVSVDGGMSDNVRTALYDATYSCTLASRASSAPPALSRVVGKHCESGDIVVRDTFL
                     PADVAPGDLLAVPVTGAYCRSLASNYNHLGRPPVVAVRDGVARLIVRRETIDDLLRLD
                     AE"
     gene            582..1883
                     /locus_tag="VII50_12615"
     CDS             582..1883
                     /locus_tag="VII50_12615"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HEY5051736.1"
                     /translation="MTGRPLRIALLGCGVVGAEVARLLTEQADDLAARVGVPLELAGI
                     AVRRLNRKRDVELDPGLFTTDAASLVAREDIDIVVEVIGGIEPARSLLLSAMSHGASI
                     VTANKALLAEDGATLYDAASKHGVDLYFEASVAGAIPLLRPLRESLAGDTVHRVLGIV
                     NGTTNFILTRMDETGAGFAEALDEATALGYAEADPSADIDAFDAAAKAAILAGLAFHT
                     RVTIADVHREGIGDVSATAVASARDMGCVVKLLAICERTADGRGVSVRVHPAMIPRSH
                     PLAGVREAYNAVFVEAEAAGQLMFYGRGAGGAPTASAVLGDIVAVARNKVKGALGPGE
                     SAYADLEIRSMGETVTRYHISLDVADKAGVLAAVATVFSAHDVSIQAVRQSGHGDDAS
                     LVIVTHAASDAALSATVEHLRTLDIVRDVASVMRVEGEETD"
     gene            1883..>2894
                     /gene="thrC"
                     /locus_tag="VII50_12620"
     CDS             1883..>2894
                     /gene="thrC"
                     /locus_tag="VII50_12620"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HEY5051737.1"
                     /translation="MTAATGPRVAQPRWRGLIEEYREWLPVTDATPVVTLQEGGTPLL
                     PAPVLSARTGCEVFLKVEGANPTGSFKDRGMTMAISKAVEEGSKAVICASTGNTSASA
                     AAYAARAGLLCAVLVPRGKIALGKLAQALVHGAKLLQIDGNFDDCLALASKLAQDYPV
                     TLVNSVNPDRIQGQKTASFEIVDALGDAPDVHCLPVGNAGNITAYWKGYVEYADAGRA
                     VKRPKMRGFQAAGAAPIVLGHVVEQPSTIATAIRIGNPASWINALDARDQSGGAIESV
                     TDRQILSAYRLLASKEAVFVEPASAASVAGLLMAHEQGTLPAGLRVVCTVTGNGLKDP
                     EWA"
BASE COUNT          482 a          944 c         1005 g          463 t
ORIGIN      
        1 cgagtgcgac gcggccaacc ttgctcatcc gaggctgagc gtcgagccag gccgcgcgat
       61 ctgcggccca acgacgttca cgctctacga ggttggcacg gtgaagccgg tcgatgtcgg
      121 tgctggagtg gttcgcacgt acgtcagcgt cgacggaggt atgagcgaca acgtccgaac
      181 cgcgctgtac gacgccacgt attcgtgcac gctcgcgagt cgggcctcaa gcgcgcctcc
      241 tgctttgagc agggtggtcg gcaagcattg cgaatcgggc gacatcgtgg tgcgcgatac
      301 cttcctgccg gcagacgtcg cccctggaga cctgttggct gtgccggtca ccggcgcgta
      361 ttgccggtcg ctggcgagca actacaacca ccttgggcgc ccgccggtcg tcgcggtccg
      421 cgatggtgtc gcgaggttga tcgtgcgtcg cgagacgatc gacgacctgc tccgcctcga
      481 cgccgagtga gccccgacca gcgtcgcgct gcccgagatg tcaggacaag gcagaatgag
      541 cgccgacgcc caccacaccg cgagcaggac ggagaaccac gatgaccggg cgaccgctgc
      601 ggatcgccct gctcggctgt ggcgttgtcg gtgccgaggt ggcgaggctg ctgaccgagc
      661 aggccgacga tctggcggcc agggtcggcg tcccgctcga gcttgccggt atcgccgtcc
      721 gccggcttaa ccgcaaacgc gatgtcgaac tcgatccggg gctgttcacc accgacgccg
      781 cgagcctggt ggcccgcgag gacatcgaca tcgtggtcga agtcatcggt ggcatcgagc
      841 ctgccaggtc gttgctgctg agtgcgatga gccacggtgc ctcgatcgtg accgccaaca
      901 aggcgctgct cgcggaggac ggcgcaactc tctacgacgc agcgagtaag catggcgtcg
      961 acctctactt cgaggcgagc gtcgccggtg cgatcccgct gctgaggccg ctgcgcgaat
     1021 cgctcgccgg cgacacggtg caccgggtgc ttggcatcgt gaacggcacc acgaacttca
     1081 tcctgacccg catggacgaa acgggtgccg gctttgccga ggccctcgac gaggccacgg
     1141 ctctcggcta tgcggaggcc gatccgagcg ccgacatcga tgcgttcgat gcggcggcga
     1201 aggcagcgat cctggccggg ctcgccttcc acacccgagt cacgattgcc gacgtccacc
     1261 gtgaaggcat cggcgatgtc agcgccactg cagtagcaag tgcccgcgat atgggttgcg
     1321 tcgtgaagct ccttgccatc tgcgagcgca ccgccgacgg tcgtggcgtc agcgtgcgcg
     1381 tgcacccggc gatgattcct cgctcgcatc cgttggcggg tgtgcgggag gcctacaacg
     1441 ccgtcttcgt cgaagccgaa gcggccggcc agctgatgtt ctacgggcga ggagccggtg
     1501 gcgcaccgac tgcgagtgcg gtactcggcg acatcgtggc ggttgcgcgc aacaaggtga
     1561 agggcgcgct cggccccggc gaatctgcct atgccgatct cgaaatacgg tcgatgggcg
     1621 agacggtcac gcgctaccac atcagcctcg acgttgccga caaggctggt gtgctcgcgg
     1681 cggtcgcgac ggtgttctcg gcccacgacg tctcgatcca ggcggtgcgt caatccggtc
     1741 atggggacga cgcgagcttg gtgatcgtga cgcacgcggc gtccgatgcg gcgctgtccg
     1801 ccacggtcga gcatcttcgc acgctcgaca tcgtccgcga cgtcgccagc gtgatgcggg
     1861 tcgagggcga ggagaccgac tgatgaccgc agccaccggc ccacgcgtcg cgcagccgcg
     1921 gtggcgcggg ttgatcgagg agtatcgcga gtggctccca gtcactgacg cgacgccggt
     1981 ggtgaccctg caagaaggtg gcacgccgct gctgcccgct cccgtgctgt cggcgcgcac
     2041 cgggtgcgag gtgttcttga aggtcgaggg cgccaacccc accgggtcgt tcaaggaccg
     2101 cggcatgacg atggcgatca gcaaggccgt cgaagaaggc tcgaaagcgg tgatttgcgc
     2161 gtcgaccggc aacaccagcg cgagcgccgc tgcctatgcg gcacgggccg gcttgctttg
     2221 cgcggtgctg gtgccgcgcg gcaagatcgc actcggcaaa ctcgcacaag ctctcgtgca
     2281 cggcgcgaaa ttgctgcaga tcgacggaaa cttcgacgac tgtctggcgc tcgcgagcaa
     2341 gctcgcccag gactatccgg tcaccctggt gaactcggtg aaccccgatc ggatccaagg
     2401 ccagaagacc gcgtcgttcg aaatcgtcga tgcgctcggt gacgcacccg acgtgcactg
     2461 tctgccggtc ggaaatgcgg gaaacatcac tgcctactgg aagggctacg tcgagtacgc
     2521 cgacgcgggg cgcgctgtca agcgaccgaa gatgcgcgga ttccaagccg ccggcgcggc
     2581 gccgattgtg ctcggccatg tggtcgagca gccgtcgacg atcgcgacgg cgatccgcat
     2641 cggcaacccg gccagctgga tcaatgccct cgatgcgcga gaccaatcgg gcggcgcgat
     2701 cgagtcggta accgaccggc agatcctgtc cgcgtaccgg ttgctcgcga gcaaagaggc
     2761 ggtcttcgtc gagccggcgt ccgccgcgag tgtggccggc ttgctaatgg ctcacgagca
     2821 gggcacgttg ccggccggcc tgcgggtggt ctgcacggtc accggcaacg gcctcaaaga
     2881 ccccgagtgg gcca
//
LOCUS       DATCBJ010000629         2572 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_534897, whole genome shotgun sequence.
ACCESSION   DATCBJ010000629 DATCBJ010000000
VERSION     DATCBJ010000629.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2572)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2572)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2572
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_534897"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..649)
                     /locus_tag="VII50_12625"
     CDS             complement(<1..649)
                     /locus_tag="VII50_12625"
                     /inference="COORDINATES: protein motif:HMM:NF012742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEY5051738.1"
                     /translation="MTLHDVATRAGVSIKTVSNVVRGVASKASPETARRVREAIDELG
                     YRPNLSARRLRSGRSQVIALALPDLRNPYFAEVAAAVIACARARDYTVFIEETGGDAA
                     AELDAAEGLSDPMIDGVILSPLLLDQKQLTARGNSVPLVLLGERQYESPCDHVMFDNV
                     EASQRITQHLIDQGFRRIAVIGHQKDPPHATAVKRLTGYRQALRGAGIAYDKTLAP"
     gene            947..2386
                     /locus_tag="VII50_12630"
     CDS             947..2386
                     /locus_tag="VII50_12630"
                     /inference="COORDINATES: protein motif:HMM:NF013697.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEY5051739.1"
                     /translation="MIPPIDPAAGSLPTSAGVTNSALSAGVSRRTVLQGGIALAAALA
                     TGGLLSGCAGGKSSSKVSSSGKLRSVSTNKSQSGTVTLWVPATDLAKVFASVIPAFNA
                     VYPNIKVNQVQVAIDTKLPPTLVSGAGVPDGAFWGDTNIPGQAAHFADLSDVMAQYKS
                     DIVPYKVDVNTVNGRLVGVPWDTDPGLLYYREDLLNAAGVDPTTLTSYDSLLTAARAL
                     KAKNPNAKPIPLEQDPGLSLQWLEMFANQQQGVGMVDKSNKLHIDSDAYRNALTWIKA
                     VSDEGLGTRQKFANTADVASLDDGTQSLCPWAVWWIFLPQGALKTSVGKWRVTSLPSW
                     TPSGARSGVMGGSSFVIPAKAKNPDLAWLFYEFLVFNKTGYTAGFGKNTTYPGGLNTV
                     VPSYTPALDPATPLFSPISQLGNEPLWQTDIAAVKQIPAGYFIPTWFNQASTYLGANL
                     QQLMDGKMSVDDVISKSSDEIQKNLINRA"
BASE COUNT          502 a          823 c          770 g          477 t
ORIGIN      
        1 ctggcgcgag agtcttgtcg taggcgatgc cggccccacg aagtgcctgc cgatatccgg
       61 tgagtcgctt gaccgccgtt gcgtgcggcg gatctttctg atgaccgatc accgcgatgc
      121 ggcgaaatcc ctgatcgatc aggtgctggg tgatcctttg ggaagcctcg acgttatcga
      181 acatcacatg gtcgcacggc gactcgtact ggcgctcgcc caagaggacg agaggtacgg
      241 agttgccacg agccgtcagt tgcttctgat cgagcagcag cggcgagaga atcacgccgt
      301 cgatcatcgg atcgctgagg ccttcggcgg cgtcgagctc ggctgccgcg tcaccgcccg
      361 tctcttcgat gaacaccgtg tagtcacgag cgcgcgcaca agcgatgacg gccgcggcta
      421 cttccgcaaa gtacgggttg cgcagatcgg gcaatgccaa cgcgatgact tgactgcgac
      481 cactgcgcag ccggcgcgcc gagaggttgg gtcggtagcc gagttcgtcg atcgcttcac
      541 ggacacggcg agcggtctct ggcgatgcct tactcgcgac tcctcggacg acattggaga
      601 ccgtcttgat cgagacacct gcgcgcgtcg cgacgtcgtg caatgtcaca gctgccacca
      661 agcctccatc tccgcgccaa ggacccgcac gtaccgctac ctgttcactg aaacaggccg
      721 ctcgataacg aaacagcgcc gattgcaacc gaccccagcg aagccgttga caccgatccc
      781 agccgcgggt acaacgatag accacaagca gctactcgtc taacccagtt ccgctccacc
      841 gcatcgagcc agccgacgga ggcgctcgct gcctgtggcg taccgagctc aaaacgttcc
      901 tcttgcaggt ctccgccgat tgttatcgct aacgaaagga tcaccgatga taccgccaat
      961 cgacccagcc gccggctctc tcccgacgag cgcaggtgtc accaactccg cactgagcgc
     1021 gggggtgagt cggcgaactg ttctgcaagg cggcatcgcg ctggccgctg ccttggccac
     1081 cggtgggctg ctatcgggat gcgcgggtgg aaagtcctca agcaaggtca gcagcagcgg
     1141 caagctccgg tccgtatcga cgaacaagtc gcagtctggc acggtgacgc tctgggtgcc
     1201 ggcaaccgac ttggcaaagg ttttcgcgtc cgtgatcccg gccttcaacg cggtctatcc
     1261 gaatatcaag gtgaaccagg tgcaggtcgc catcgacacg aagctgccac ctacgcttgt
     1321 gagtggcgcg ggcgtccccg atggcgcgtt ctggggcgac accaatattc ccggccaggc
     1381 ggcacatttt gcagatctga gcgacgtcat ggcccaatac aaatctgaca tcgtgcctta
     1441 caaggtcgac gtcaacaccg tcaacggccg gctggtcggc gtgccgtggg acaccgatcc
     1501 cggcttgctg tactaccgcg aggatctcct gaacgcggcc ggagtcgacc cgacaacgct
     1561 gacttcctac gacagcctgc tcaccgcggc ccgcgccttg aaggccaaga accccaacgc
     1621 aaagccgatt ccactcgagc aggatcctgg cctgagcctg cagtggctgg agatgttcgc
     1681 caaccaacag cagggcgtcg gcatggtcga caagagcaac aagctgcaca tcgactccga
     1741 tgcgtatcgc aatgcgctga cctggatcaa ggccgtctcc gacgaagggc tcggcacacg
     1801 gcagaagttc gccaacacgg cggatgtcgc ttcgctggac gacggcacgc agtcgctgtg
     1861 cccctgggca gtctggtgga tcttcttgcc ccaaggggcc ttgaagacaa gcgtcggaaa
     1921 gtggcgagtg acatcgcttc cgtcgtggac tcctagcgga gcgcgcagtg gggttatggg
     1981 tggaagctcc ttcgtgatcc cggctaaagc caagaacccc gacctggcat ggcttttcta
     2041 tgagttcctg gtgttcaaca agaccgggta taccgccggg ttcggcaaga acacgacgta
     2101 cccgggcggc ctcaacaccg tcgtcccgtc gtacacgccg gctctcgatc ccgcgacgcc
     2161 gttgttcagc ccgatttcac aactgggtaa cgagcccctg tggcagaccg acatcgccgc
     2221 ggtgaagcaa atccccgctg ggtatttcat accgacctgg ttcaaccagg ccagcactta
     2281 cctcggtgcc aacctgcagc agttgatgga cggcaagatg agcgtcgacg acgtgatcag
     2341 caagtcgtcg gatgaaatcc agaagaatct catcaaccgc gcctaagggt tatcgccgtg
     2401 agttctgttg gacttgatga cacacagctg gtcgcaccgc cggcgcgtat cgtgccgcgc
     2461 cggcggtggc gatccgatcc gcgatggtcg ggcttcttgt tcgtcctgcc atttctagtt
     2521 atcttcgctg ccttcggtgc ctaccccttg ctgtatgcgc tgcggatcag ct
//
LOCUS       DATCBJ010000630         2255 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_240269, whole genome shotgun sequence.
ACCESSION   DATCBJ010000630 DATCBJ010000000
VERSION     DATCBJ010000630.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2255)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2255)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2255
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_240269"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..525)
                     /locus_tag="VII50_12635"
     CDS             complement(<1..525)
                     /locus_tag="VII50_12635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HEY5051740.1"
                     /translation="MVDYEMRVDMDRLRSYRLGRARAALEASDCGSFLLFDFYNIRYV
                     TQTWIGGALGDKMIRYALVFRDRDPILWDFGSAVRHHKLYSTWLPDENWRPGFLGFRG
                     AVAPEAGLMRDAVTEIKSLLTEAGVAGAPLGLDIVEPPFLFELDRQGITVRDAQQAML
                     DARVIKSPDEIMLLN"
     gene            777..2183
                     /locus_tag="VII50_12640"
     CDS             777..2183
                     /locus_tag="VII50_12640"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY5051741.1"
                     /translation="MASRTTVDADDWPMRPLAFLVAGTLFMENLDATIVATAAPSIAR
                     SFGVTSSQIGITATAYLATVAALIPVSGWLAARRGARQTFLFAIALFTVASVLCSFST
                     NLTELSIMRVAQGAGGAMMVPVGRFVVLRATGKSNIIKAVAYLTWPALVAPVLAPAVG
                     GLITTYATWPWIFLINLPLGIVALSVALRIMPRQARGDPGPLDWIGFVSTAISLGCLV
                     VLAADLGSPTVSWLAVAVLAGLTVGVGTFAIVHLLRARSPLVALDALRVKTFRAAAVG
                     GTAFRVGINGAAFLLPLLFQDGFGWSPARAGSTLLFLFLGNLLIKPATTPLLRVAKFR
                     TVLVATTAGATLTLIGCALLRPSTNVMLLIGVLALSGVFRSIGYTGYNTIAFADIDQA
                     SMSQANTLASTIQQLTQAFGVALAVISLKVGTGIVGHGNNFRFAFCVLGVAVFCALVA
                     AVRLPVDSGDALRGGRKA"
BASE COUNT          351 a          754 c          701 g          449 t
ORIGIN      
        1 gttgagcagc atgatctcgt ccggcgactt gatgacgcgg gcgtcgagca tggcctgctg
       61 ggcgtcgcgc accgtgatgc cctgccggtc cagctcgaac aggaacgggg gctcgacgat
      121 gtcgaggccg agcggggcgc cagccacccc ggcctccgtg agcagcgact tgatctcggt
      181 caccgcgtcg cgcatcaggc ccgcttcggg ggcgaccgca ccgcggaagc cgaggaaccc
      241 gggcctccag ttctcgtccg gcagccacgt cgagtacagc ttgtgatgcc gcaccgccga
      301 gccgaagtcc cacagaatcg ggtcccggtc ccggaacacc agggcgtagc ggatcatctt
      361 gtcgccgagc gccccgccga tccaggtctg cgtgacatac cgaatgttgt agaagtcgaa
      421 gagcagaaac gagccacagt cgctcgcctc aagcgcggca cgtgcacgcc cgagccggta
      481 cgaacgcagt cggtccatat caacgcgcat ctcgtagtcg accaagccgt gtccgggggc
      541 tgggatctgc ggggtgcgaa agatcgccgc gtctattgcc gagagggagg aatccgacat
      601 ctagcttccc ccttctcctc gcgaaggccc gcgtcgacct cgggctactt tcaccatgca
      661 aacgattgct gttagagtag catcggccgc tcgcggctcg tcaactagtc ccgcgtgaga
      721 ctcaactttc gagctcagcc ggcctgcctc tcggcgcgat gacggaggcc catcgggtgg
      781 ctagccgcac aacggtcgat gccgacgact ggccgatgcg tccgctcgca ttccttgtag
      841 ccggcacgct cttcatggag aacctcgacg ccaccatcgt cgctacggcc gcaccgagca
      901 tcgcccgatc cttcggcgtg acgtcatcgc aaatcggcat cacggcaacc gcataccttg
      961 cgaccgtcgc cgcgctcatc ccggtgagtg gctggttggc tgcgcggcgc ggtgcgcggc
     1021 agacgtttct cttcgccatc gctcttttca cggtggcgtc ggtgctctgc tccttcagta
     1081 ccaacctcac cgagctgagc atcatgcggg tggcgcaggg cgcgggcgga gcgatgatgg
     1141 tgccggtcgg acgattcgtg gtcctgcgtg ccaccgggaa atccaacatc atcaaggccg
     1201 tcgcctacct cacctggccc gcacttgtgg ctccggtact ggcgcccgcg gtaggtgggc
     1261 tcatcacgac gtacgcaacg tggccctgga tcttcctgat caacctgcca ctgggcatcg
     1321 tcgcgcttag tgtcgctttg cggatcatgc ctcggcaagc gcgtggcgac cccggcccgc
     1381 ttgactggat cggctttgtc agcaccgcga tttcgctcgg ctgccttgtc gtgctagccg
     1441 cggacctggg ctcaccgacg gtgtcctggc tagctgtggc cgtcctcgcg gggctgaccg
     1501 tgggagttgg cacgttcgca atcgtccatc tcctgcgggc tcgatcgccg ctcgttgcac
     1561 tcgacgcctt gcgggtgaaa acatttcggg ccgccgcggt gggtggaact gcttttcggg
     1621 tgggcatcaa cggcgccgct tttctgctcc ccttgctgtt ccaggacggt ttcgggtggt
     1681 ccccggcccg ggccggatca acgcttttgt tcctcttctt aggtaacttg ctcatcaagc
     1741 cggcaacaac tccgctgctg cgagttgcaa agtttcgaac tgtgctagtc gcgacgactg
     1801 ccggcgccac gctcacactg atcggctgcg cactcctgcg gccatcgacg aacgtgatgc
     1861 tgctcatcgg cgtcctcgcc ctgagcggcg tcttccgttc gatcgggtat accgggtaca
     1921 acacgatcgc cttcgccgac atcgatcagg cgtcgatgag ccaggcgaat acgcttgctt
     1981 caacgattca acagctgacg caagcattcg gcgtcgcgct cgctgttatc tccctcaagg
     2041 ttggcaccgg catcgtcggt cacggaaaca acttccggtt cgccttctgc gtgctcggcg
     2101 tcgctgtatt ttgcgcgctg gtcgcagcgg tacgcctgcc ggtcgattct ggcgacgccc
     2161 tacgcggcgg ccgtaaagcc taggttgcca gctgcttgtt ggcctgctgt tcaagccgtt
     2221 cggcgacatc gggagcaaga ccggccgtgc ctgcg
//
LOCUS       DATCBJ010000631         5528 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_129799, whole genome shotgun sequence.
ACCESSION   DATCBJ010000631 DATCBJ010000000
VERSION     DATCBJ010000631.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 5528)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5528)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5528
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_129799"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1259)
                     /locus_tag="VII50_12645"
     CDS             complement(<1..1259)
                     /locus_tag="VII50_12645"
                     /inference="COORDINATES: protein motif:HMM:NF012431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HEY5051742.1"
                     /translation="MTTVADFVVQRVRQWGVTRVFGFPGDGVGELDGALGQAEHDGDG
                     LSYIRPTHEEICALMAVAHAKFTGEVGVCIATSSPGAFHLINGLYDANMDNQPVVAIV
                     GQQGLNALGTFNQQESNLERTFADVAAYVQTIVSPQQAQAVIDTAFRTARVRLAPTVI
                     IIPHDVQAMEMEEPAAATWVSRSSAVAPSIAITPPQSEIIKAAGIINSGSKVTFLVGH
                     GANGATDEVIEAARLAGAGIITALRGKQVVPSDIAFHSQQLGLLGSRPSADQMAGCDT
                     LLLLGTNYPYGQFLPKTGQARAIQVDLKPEQMGLRYPTEVNLWGDVKSTLTALIPHLQ
                     AKTDLGWQNTVAEGMLEWEAEMEAQAMLTYPDGVNPRRVYHELNKRLPPNAIVTADAG
                     TTADWYGHHIRLRRGMMGDLSGRLATML"
     gene            1607..2554
                     /locus_tag="VII50_12650"
     CDS             1607..2554
                     /locus_tag="VII50_12650"
                     /inference="COORDINATES: protein motif:HMM:NF013431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HEY5051743.1"
                     /translation="MRHEATGDQVPSRPAMPSGQQFHISHGEHHATVVEVGGGLRRYD
                     VAGRELLDGYPSEERCTGARGLPLVPWPNRIQDGAYAFDGIDYQVPLTEPEAHNAIHG
                     FGRWRNWTCHQHTESQVVMGTVLHPQMGYPFTLDVRVEYQLTAAGLTIRTAATNIGAQ
                     PCPYGAGQHPYLSLGTDLIDDCQLHLDAARWLPTDERGLPTGSADVQGSDYDFRSPKQ
                     IGARDIDYTFTDLARDRDGLAWVHLSAATHGALSLWVDESYPYIEIYTAHTQPAPHWR
                     TGLGVEPMTCAPNAFRSGQGLIRLAPGQAHTASWGLRPA"
     gene            2756..4126
                     /locus_tag="VII50_12655"
     CDS             2756..4126
                     /locus_tag="VII50_12655"
                     /inference="COORDINATES: protein motif:HMM:NF005495.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY5051744.1"
                     /translation="MALLRGVWGVVELAVPRKLLASLGSVDTGRLPVGVLRVLGMRHV
                     LQAAATFALPDEAVVKLGAGADALHALTGFGLAATSRSWRRAALTDSFVAVSFSAAGW
                     LLRPRSTEFSDRNMEGCKGMTARKVAVVTGGSAGVGRAVVRELANHGWDVGVLARGQA
                     GLDGAVADVQARGGKGVGLPTDVAVLDQVRAAADRIENELGPIELWVNVAFSGSLRFF
                     WDTDDDVFRRITEVTYLGMVNGTRVALERMRPRNKGVIVQTGSALAYRGIPLQSAYCG
                     AKHALVGFTESVITELKHEKSAVRVCMVQLPAVNTVQFDWNDSEFEEHPMPVPPIFQP
                     EVPARAVRYLADHPRRNLWVGASTAGTIVGNRLAPGLLDWYLGRTGVKSQTTKQDGPR
                     YGSNVFQARDDEQDRGAHGMFDDQAHASDPWSAVSMRRTPIAAGVLGSLVGAALLALK
                     VTRRTR"
     gene            4123..>5528
                     /locus_tag="VII50_12660"
     CDS             4123..>5528
                     /locus_tag="VII50_12660"
                     /inference="COORDINATES: protein motif:HMM:NF012926.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
                     /protein_id="HEY5051745.1"
                     /translation="MRPQRLDGWVPIADYGLLSNGHTVAMVALDGRIDWWPLPALNDP
                     PVFAAILDPINGGRLEMQPADDFTVTRRYVGESNVLETTFRTTTTTGTARVTDALTVG
                     RAGVPLPWGELVRRVDGVDGQVAMRWAVYPGTRFGTTEPWTQQSGDDIVVHCADQHLA
                     LRCFDIGKPKVTAHQVSGTFRTSATSTGIFAISASDNAPIYLPDRAALESRLDDTVSS
                     WHQWSAAMTYDGPWSEAVHRSGLVLKLLLYGPSGAIAAAATTSLPERIGGDKNWDYRY
                     TWIRDTAFTVEACIQLGLEEEVQGAVASLLRSVRSTLPGLNVFYQLDGGVPAKEHDLA
                     APGYRNSRPVRAGNAAVSQTQLGTFGDLFDSVWRYVGDGHLLDPASAQMLAELADRCC
                     DIWHSKDAGIWELNEKQHYTISKIGCWVALDRAVRLHEHGQVPTAHADRWRAERDGIK
                     AWVNEHCWSADKCSYTFFA"
BASE COUNT          936 a         1786 c         1884 g          922 t
ORIGIN      
        1 agcatcgtcg ccagtcggcc ggacaggtcg cccatcatgc cgcggcgaag tcggatgtga
       61 tggccgtacc agtctgcggt cgtgccggcg tcagcggtga cgatggcgtt gggtggcagc
      121 cgcttgttca gctcgtggta gacgcgccga gggttcacgc cgtcggggta cgtgagcatc
      181 gcctgggctt ccatctcggc ttcccactcg agcattccct cggcgacggt gttctgccag
      241 ccgagatcgg tctttgcctg caggtgcggg atcagcgcgg tcagggtcga tttgacgtcg
      301 ccccacaggt tgacttcggt ggggtagcga aggcccatct gctcgggttt caggtcgacc
      361 tggatcgctc gcgcctgacc ggtcttgggc aggaactggc cgtacgggta gttagtgccg
      421 agtagaagga gcgtgtcgca accggccatc tggtccgcgc tggggcgcga gccgagcagt
      481 cctagttgct gggagtggaa agcgatgtcg gaggggacca cttgctttcc gcgcagcgcg
      541 gtgatgatgc cggcaccggc cagacgcgcc gcttcgatca cttcgtcggt tgcgccgttg
      601 gcgccgtgac cgacgaggaa ggtgaccttc gagccgctgt tgatgattcc ggccgccttg
      661 atgatctcgc tctgcggcgg cgtgatggcg atcgatggcg cgacggcact ggagcgcgac
      721 acccaggtcg cggccgcggg ttcttccatc tccatggcct gaacgtcgtg cgggatgatg
      781 atgaccgtcg gcgcaagtcg aacgcgtgcg gtgcgaaacg ccgtgtcgat gacggcttgc
      841 gcctgctggg gcgacacgat cgtctgcaca tacgcggcga cgtcggcgaa ggtgcgctcg
      901 aggttgctct cctgctggtt gaacgttccc agagcgttca ggccctgctg gcccacgatg
      961 gcgacgactg gttggttgtc catgttggcg tcatacaggc cattgatcag atggaacgcc
     1021 ccagggctcg acgtcgcgat gcacacgcca acttcaccgg tgaacttggc gtgcgccacc
     1081 gccatcagcg cgcatatctc ttcgtgggtg ggtcggatgt agctgaggcc atcaccgtcg
     1141 tgctccgcct gaccaagcgc gccgtcaagc tcccccactc cgtcgccggg gaacccgaat
     1201 actcgcgtca ccccccactg cctgacgcgt tgaacgacga agtcggcgac tgttgtcatg
     1261 gcaggtctcc cgattcagcg gcgagcggac tcccggcgcg agaggcgcaa cgcgaagcca
     1321 gcactcctgt ttgaatgcgg ccctacccgg cgtcggcatg accaaacagg cgccagctaa
     1381 taagcgatgt cgccgaggat cgttggttag tcagcgcgga gcaatgcacg tgcagtcgaa
     1441 gagcaaaggg ggcgccaagc aacgcgtcca ttcagctccg cggcgacgac cggaacatac
     1501 tcatctcacc ggcgtatgtg ctggcagcac agtgggacag cggcagcaca gtagaccgag
     1561 tgtcgcaacg cgtaaccggc atcgtcgcaa aagaaggagc agtccgatga ggcacgaggc
     1621 gaccggggac caagtcccat cccggccggc gatgccgtcc ggacagcagt tccatatcag
     1681 ccacggcgag catcacgcga ccgtggtcga agtcggcggt gggctgcgcc ggtatgacgt
     1741 ggccggacgt gaactgctcg acggctaccc tagcgaggag cggtgcaccg gcgcgcgggg
     1801 tctgccgctg gtcccctggc ccaaccgcat ccaggacggc gcctacgcct tcgacgggat
     1861 cgactaccag gtgccgctca ccgaacccga agcgcacaac gccatccacg gattcgggcg
     1921 ctggcgaaac tggacctgcc accagcacac cgaaagccag gtcgtgatgg gaaccgtgct
     1981 gcacccgcag atgggctacc cgttcaccct cgacgtccgc gtcgagtacc agctcaccgc
     2041 ggccgggctg acgatacgca ccgccgccac caacatcggt gcgcagccct gcccctacgg
     2101 ggccggccaa cacccctacc tgagcctagg caccgacctc atcgacgact gtcagctgca
     2161 ccttgatgcc gcccgatggc tgccgaccga cgaaagaggt ctgccgaccg ggtcggctga
     2221 cgtgcaggga tcggactacg acttccgcag cccaaaacag atcggtgccc gcgacatcga
     2281 ctacacgttc accgacctcg cccgcgaccg cgacgggctg gcatgggtac acctgtcggc
     2341 agccacgcac ggcgcgctat ccctgtgggt cgacgaaagc tacccctaca tcgagatcta
     2401 caccgcccac acccaacctg ctccgcactg gcgcacgggg ctgggcgtag aaccgatgac
     2461 ctgcgcgccc aacgcgttcc gcagcggcca aggcctgatc cgcctcgcgc ccggacaggc
     2521 ccacactgcg agctggggcc tgcgcccggc gtaagcaatc agccgccgct ccgatcgagg
     2581 cctgccgact cagctcgtcc ttggtcgtca gaccagccga ccgtcctgga atagctgccc
     2641 aaccgcgacg atccgagcgg gtaactcgcg tgtgttcggg tagggcccat gggtgacgca
     2701 gcgcaagggg aggctcgggt gtgggagggg tagcgcgacc gctccgacac gggtgttggc
     2761 gcttctgcga ggtgtgtggg gcgtcgtcga acttgcggtg ccccggaagt tgcttgcgtc
     2821 tcttgggagc gtcgacacgg gccggcttcc ggtcggtgtg ctgcgagtcc tcgggatgcg
     2881 gcacgtactt caggcagcgg caacgtttgc cctgcccgac gaggcggtgg tcaagctcgg
     2941 cgctggggcc gatgccttgc acgccttgac cgggttcggg ctagccgcga cgtcgcgctc
     3001 gtggagacgg gctgctctga cggacagctt cgtggccgtc agcttcagtg ccgcgggctg
     3061 gttgctgcgg ccacgctcaa ccgagttctc agaccgcaac atggagggat gtaaaggcat
     3121 gactgcgcgc aaagtcgctg tcgttaccgg aggctccgcg ggggtcggcc gtgccgtcgt
     3181 gcgcgagctc gctaaccacg gctgggacgt cggtgtgctg gcccgcgggc aggctggcct
     3241 ggatggcgcc gtcgcggacg tgcaggcccg tggcggcaag ggcgtaggcc ttcccaccga
     3301 cgtcgccgta ctggatcaag ttcgagccgc agcggatcgg atcgagaacg agctcggccc
     3361 gatcgagctg tgggtcaacg tggcgttctc cggctcgctg cggttcttct gggacaccga
     3421 cgacgacgtg ttccgtcgga ttaccgaggt gacctatctc ggcatggtca acggcactcg
     3481 ggtggcgctg gagcgcatgc gtccgcgtaa caagggcgtg atcgttcaga ccggctcggc
     3541 acttgcctac cgcggaattc ctttgcagtc ggcgtactgc ggagccaagc acgccctcgt
     3601 cgggtttacc gagagcgtta ttactgagct caaacatgag aagagcgccg tgcgggtgtg
     3661 catggtgcaa ctgcccgcgg tgaacacggt gcagttcgat tggaacgaca gcgagttcga
     3721 ggaacatccg atgccggtgc cgccgatctt ccagccggag gtgccagccc gtgcggtgcg
     3781 gtacctggcc gaccacccgc gccgcaacct gtgggtcggc gcgtcgaccg cgggcaccat
     3841 cgtcggcaac cggctggcgc cgggactgtt ggactggtat ctgggcagga ccggggtgaa
     3901 gagtcagacc accaagcagg acggcccgcg gtacggctcc aacgtgttcc aggccaggga
     3961 cgacgaacag gaccgcggcg cgcacgggat gttcgacgac caggcgcacg cctcggatcc
     4021 ttggtcggcg gtgtcgatgc gtcggacgcc gatcgcggcc ggggtcctcg gcagcctcgt
     4081 cggcgcagcc ctactcgcgc tgaaagtgac gcggcgtacg cggtgaggcc gcagcggctc
     4141 gacgggtggg tgccgatcgc ggattacggt ctgctgagca acggtcatac cgtggccatg
     4201 gtggcgctgg acggccggat cgactggtgg ccgctcccgg cgctgaacga cccgccggtg
     4261 ttcgcggcaa tcctcgaccc gatcaacggt gggcgcctcg aaatgcagcc ggcggacgat
     4321 ttcaccgtga cgcgacgtta cgtcggcgag tccaacgtcc ttgaaacgac gttccgcacg
     4381 accacgacca cgggcacggc acgggtgacc gatgccctga ccgtcggccg ggccggtgtg
     4441 ccgctgccgt ggggcgagtt ggtccggcgc gtcgacggcg tcgacgggca ggtcgcgatg
     4501 cggtgggctg tgtatcccgg caccagattc ggcaccacgg agccgtggac gcagcaaagc
     4561 ggagacgaca tcgtcgtgca ctgcgccgac cagcacctgg cgctgcgctg cttcgacatc
     4621 gggaaaccga aggtaaccgc acaccaggtc agcggcacat tcaggacctc ggcaaccagc
     4681 accgggatat tcgcgatctc ggcgagcgac aacgccccga tttacctgcc cgaccgggcg
     4741 gctttggagt cacggctcga cgacaccgtc agcagctggc accaatggtc agccgcgatg
     4801 acctacgacg gcccgtggtc cgaagcggta cacaggtccg gtctggtgct caagctgttg
     4861 ctttacggcc cgagcggtgc gatcgctgcg gcggcgacca cgtcgttgcc ggagcggatc
     4921 ggcggggaca agaactggga ctaccgctat acgtggatcc gcgacaccgc cttcaccgtc
     4981 gaggcatgta tccaactcgg ccttgaggag gaggtgcagg gtgcggtcgc gtcgctgttg
     5041 cgatcggtcc gttcgaccct tcccgggctg aacgtgtttt accaacttga cgggggtgtg
     5101 ccagccaagg agcacgacct cgccgcaccg ggctaccgca atagtcgccc tgtccgggct
     5161 ggtaacgcag ccgtgtccca gacccagctc ggcaccttcg gggatttgtt cgattcggtc
     5221 tggcgctacg tgggcgacgg tcacctcctc gaccccgcca gcgcgcagat gctcgccgag
     5281 ttggccgacc ggtgctgcga catctggcat tcgaaggacg caggcatctg ggagttgaac
     5341 gagaagcagc actacacgat ctcaaagatc ggctgctggg tcgcgctcga ccgggcagtt
     5401 cggttgcacg agcacggcca ggtacctacc gcgcatgccg accgctggcg cgccgaacgt
     5461 gatggcatca aagcgtgggt caacgagcac tgctggtcgg cagacaaatg cagctacacg
     5521 tttttcgc
//
LOCUS       DATCBJ010000632         5418 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_19259, whole genome shotgun sequence.
ACCESSION   DATCBJ010000632 DATCBJ010000000
VERSION     DATCBJ010000632.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 5418)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5418)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5418
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_19259"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..274
                     /locus_tag="VII50_12665"
     CDS             <1..274
                     /locus_tag="VII50_12665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5051746.1"
                     /translation="VNSIAVAHPAENAIFGLRVSALVELGLALLAVLLAYLAHSAARI
                     DPDLTAHRVARWMAGAALLLAIVSIAESGASLLIMLGAHAPAVSGG"
     regulatory      277..387
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            380..1516
                     /gene="thiO"
                     /locus_tag="VII50_12670"
     CDS             380..1516
                     /gene="thiO"
                     /locus_tag="VII50_12670"
                     /EC_number="1.4.3.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016647 - oxidoreductase activity, acting
                     on the CH-NH group of donors, oxygen as acceptor [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine oxidase ThiO"
                     /protein_id="HEY5051747.1"
                     /translation="MAGHDGDVVVVGGGVIGLAIAWRAALRGLAVSVLDPTPGMGASH
                     HGAGMLAPVTEVHYGEEDLLRLNLASATRYPSFVAELEEASGTPVGYRATGTLAVAFD
                     ADDKAALDELHDYQSSLGLSSQPVTARECRKLEPYLSTAVRGGLLVDGDHQVDPRRLV
                     AALLVACTRAGVDLRRVPARHVRVEGDRVVGVDDLSTRTVVLAAGCWSGELRGLPNHA
                     VPPVRPVKGQILRLRDDVEHLTLQRNVRALVRGRPIYLVPRADGEIVLGATSEEKGFD
                     TRLTVEGTGDLLADARELLPRVSEIELLEASVGLRPGSPDNAPMIGAGALPGLVVATG
                     HYRNGILLAPTTADGVAELLTSGALPAGFERFDPCRFARAAVPA"
     gene            1513..1713
                     /gene="thiS"
                     /locus_tag="VII50_12675"
     CDS             1513..1713
                     /gene="thiS"
                     /locus_tag="VII50_12675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003110013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HEY5051748.1"
                     /translation="MTIVVNGEHHDLPDDAMLADAIARLTTGMRGVAVALNGSVVPRG
                     EWDSTALVDADQVEILTAVQGG"
     gene            1717..2475
                     /locus_tag="VII50_12680"
     CDS             1717..2475
                     /locus_tag="VII50_12680"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990107 - thiazole synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HEY5051749.1"
                     /translation="MDDPFVLAGETFTSRLIMGTGGIPSLDVLEAALEASGTEITTVA
                     MRRVDPTAHGSILDVLARRGVRVLPNTAGCYTAADAVLTAQLGREALGTDWVKLEVIA
                     DEHTLLPDPIELLNAAETLVSEGFLVLPYTNDDPVLARRLEQAGCVAVMPLGSPIGSG
                     LGIRNPHNIELIVERAGVPVVLDAGIGTASDAALAMELGCDAVLIASAVTRAQRPALM
                     ADAMRKAVEAGRLARLAGRIPVRRYAKASSPPVD"
     gene            complement(2442..3155)
                     /locus_tag="VII50_12685"
     CDS             complement(2442..3155)
                     /locus_tag="VII50_12685"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HEY5051750.1"
                     /translation="MHPGVVLFDLDGTLTESGPGIMNSLAYALDAIGRPPLGAAELRR
                     FVGPPLHESLRDVAGLDDARTHDAMIAYRRYFVAHGMYENSVYPGIVDLLRALGDAGR
                     RLGVATSKPIPYALPIVEHFDLRPYFEVVCGPVLDGVGASKAEVVADALASLAVGAGS
                     DVVLVGDRSHDVVGAHENGIGCIGVLWGYGSRDELTAAGADAIAADVDELAALLGVRL
                     PASGPAALDQSTGGDDAFA"
     gene            3274..5244
                     /gene="pknB"
                     /locus_tag="VII50_12690"
     CDS             3274..5244
                     /gene="pknB"
                     /locus_tag="VII50_12690"
                     /inference="COORDINATES: protein motif:HMM:NF033483.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HEY5051751.1"
                     /translation="MSDALVGRVLDGRYRVEARIARGGMATVYRALDTRLDRVVALKV
                     MHQLFAENDQFVARFIREAKSAARLSHPNVVAVYDQGDDGGYVFLAMEYVQGRTLRDL
                     LRERTRLTPQEALWILEPVLAALSAAHAAGLVHRDVKPENVLLADDGRVKVADFGLAR
                     AAANLEATSATSLIGTVAYLSPEQVIRGIADERSDVYSAGIMLFEMLTGRPPYDGETA
                     VSVALKHAHEDVPPPSSLVSGIPTSVDLLVARATARDADRRPHDAGAFLAEVVRVRGG
                     LVASDADAGSLTSLINRTLVVELPDTASVGPNPPWQLGQAPVPAQPPYGGQPGQPGQP
                     PRIVEPPQPPEEEYRRPHPHRNGWIAFFIVLLLAAAAAGAGWWFAAGRWASTPNLVNL
                     SSSAATAKAKAAHVTVQVGQPEFSSTVPKNSVVEQTPTAKHKVLRGDAITIRLSQGPQ
                     LFQVPTYDLAGNTSLVDYQSELIAAHLNVTDPPVTEYSPTVIAGNVISVDPPVGTNEK
                     AGTAVTITVSLGPQPIPIVDYTGQPADQATQALTTAGFTVVPTQDYSTAVKAGLVISQ
                     SPSSGSGKLGDTVALDISKGPQLFKVPDVTSQLNGDPKNWVSIQQATKTLTDAGFKVN
                     VGSSGRFGVVTSQSPKQLSMQPLGTVITIYAQ"
BASE COUNT          875 a         1895 c         1782 g          866 t
ORIGIN      
        1 cgtgaactcg atcgcagtgg cgcatccggc ggagaatgcc atcttcggac tccgggtcag
       61 cgctcttgtc gaactcggcc tcgccttact cgcggtgctt ctcgcctacc ttgcccacag
      121 cgcggcccgc atcgatcccg acctcacagc gcatcgggtc gcacggtgga tggcgggcgc
      181 agcactgctt ctcgcgatcg tgtcgatcgc cgaatccggc gcatccctgc tcatcatgct
      241 cggcgcgcac gcccccgccg ttagcggcgg ttagactgca gattgcacgg gagcccggtc
      301 ggactgggct gagagggcgg ctggtggccg ccgaccgtcg aacctgctcc gggtcatgcc
      361 ggcgaaggga gtcaatgcca tggccggcca cgatggtgac gtcgtcgtcg tcggcggtgg
      421 cgtcatcggc ctcgcgatcg cctggcgcgc cgcgctgcgc gggctcgcgg tttccgtcct
      481 cgatccgact ccaggcatgg gcgcctcgca ccacggcgcg ggcatgcttg caccggtcac
      541 cgaggtgcac tacggcgagg aagacctcct tcgcctaaac ctcgcgtcag caactcgcta
      601 cccgagcttc gtcgccgaac tcgaagaagc cagcgggacg ccggtgggtt accgcgcgac
      661 cggcacgctc gccgtcgcgt tcgacgccga cgacaaggcg gcactcgacg aattgcacga
      721 ctaccagtcc tcgctgggtc ttagctcgca gccggtcacg gctcgcgagt gcaggaaact
      781 cgagccctac ctgtccacgg ccgtccgcgg cggccttctt gtcgacggtg accaccaagt
      841 cgatcctcgt cgcctcgtcg ctgcgttgct ggtggcgtgc acgcgagctg gcgtcgatct
      901 gcgccgcgtc cctgctcgcc acgttcgagt cgagggcgac cgcgttgtgg gcgtcgacga
      961 cctcagcacg cgaaccgtcg tgctcgccgc cggctgctgg tctggtgagt tgcgcgggct
     1021 ccccaatcat gcggtgccgc cggtgcggcc ggtaaagggt cagatcctgc gactgcgcga
     1081 tgacgtcgag catctgacgc ttcaacgcaa tgtgcgcgca ctcgtacgcg ggcggccgat
     1141 ctacctcgtg ccgcgtgcag acggtgagat cgtgctcggc gccaccagcg aggaaaaggg
     1201 attcgacacc cgacttaccg tcgagggcac cggcgacctg ctagccgacg cccgagaact
     1261 gctgccgcgg gtaagcgaga tcgaactgct cgaggcatcg gtcggactac gtccgggctc
     1321 accggacaac gcgcccatga tcggcgccgg tgcactgccc gggctcgtgg tggcgaccgg
     1381 ccactaccgc aacggcattc tgcttgcgcc gaccacagcc gacggtgttg cggagttgct
     1441 cacgtcgggc gccttgccag ccgggttcga gcgtttcgac ccgtgccgct ttgcgcgagc
     1501 cgcggtgccg gcatgactat cgtcgtgaac ggtgagcacc atgacctgcc tgacgacgcg
     1561 atgctggccg acgcgatcgc ccggctcaca acggggatgc gcggcgtcgc cgtcgcgctg
     1621 aacggcagcg tggtcccccg cggcgagtgg gactccacgg ccctggtcga cgccgatcaa
     1681 gtcgagattc tgactgctgt gcaagggggt tgagccgtgg acgacccgtt cgtgctggcc
     1741 ggtgagacgt tcacctcgcg cctgatcatg ggaaccggcg gcattccgag cctcgacgtc
     1801 ctcgaggcgg cgctcgaagc gtcgggaacc gagatcacca cggtggcgat gcgccgcgtc
     1861 gatccgactg ctcatggttc gatcctcgac gtactcgccc ggcgtggtgt gcgcgtgctg
     1921 ccgaacaccg cgggctgcta caccgcagca gacgccgttc tcacggcgca gcttggtcgc
     1981 gaagcactgg gtacggactg ggtgaagctc gaggtgatcg ccgacgagca caccttgctg
     2041 cccgacccga tcgagctgct caacgcggca gaaacccttg tgtccgaagg ttttctggtg
     2101 ctgccgtaca cgaacgacga tccggtgctt gcacgccgtc tcgaacaggc tggctgcgtg
     2161 gccgtcatgc cgctgggctc cccgatcggt tccgggctcg gcatccgcaa tccgcacaac
     2221 atcgagctga tcgttgaacg cgccggggta cctgtcgtcc tcgacgccgg catcgggacc
     2281 gcgagtgacg ccgcgctggc gatggaactg ggctgcgacg cagttctgat cgcctcggcc
     2341 gtcacccgcg cgcaacgccc tgcgctgatg gccgacgcga tgcgcaaggc cgtcgaagcc
     2401 ggccgcctgg cgcggctcgc cgggcggatc cccgttcgcc gctacgcgaa agcgtcgtca
     2461 ccaccagtcg actgatctag ggcagcgggg cccgacgcag ggagccggac gcccagcagt
     2521 gccgccagct cgtcgacgtc cgcggcaatg gcgtcagcgc ccgcggcggt cagctcgtcg
     2581 cgactgccgt agccccacaa cacacctata catccgatcc cgttctcatg ggctccgacc
     2641 acgtcgtgcg aacggtcacc aacgaggacg acgtcagagc cggcgcccac cgcaaggctc
     2701 gccagcgcat cggcgaccac ttccgccttc gacgctccta cgccgtcgag cacgggcccg
     2761 cagacaacct cgaagtacgg ccgcaaatcg aagtgctcga cgatcgggag cgcatacggg
     2821 atcggtttcg acgtcgcgac cccgagccgg cggccagcat cgccgagcgc gcgcaagagg
     2881 tcgacgatac cggggtacac ggagttctcg tacatcccgt gcgcgacgaa gtaccgacgg
     2941 taggcgatca tcgcgtcgtg cgtcctggcg tcgtcgagac cggcgacgtc gcgcaacgat
     3001 tcgtgcagcg gcggcccgac aaagcggcgt agttcggcag cgccgagcgg cggccgcccg
     3061 atcgcgtcga gcgcataggc gagcgagttc atgatgcccg ggcctgattc ggtcagcgtc
     3121 ccgtcaagat cgaagaggac gacgccggga tgcacagcgc ccatcctgtc gtacaaagcg
     3181 ccggggacca cccgcgtcca gggccacgtt ccgcgtcgcg cctgccccgt cgagccgacc
     3241 gtcgcacgta tgctcgacgc cgtggatacg acgatgtcgg acgccttggt gggccgcgtc
     3301 cttgacggcc ggtaccgggt cgaggcacgc atcgcccgcg gtgggatggc gacggtgtac
     3361 cgcgcgctcg acacccgatt ggaccgcgtc gtcgcgctca aggtcatgca ccaactgttc
     3421 gccgagaacg accagttcgt cgcgcggttc atccgtgagg ccaagtcggc tgcgcgcctc
     3481 tcgcacccca acgtcgtcgc ggtctacgac cagggcgacg acggcggcta cgtgttcctt
     3541 gcgatggagt acgtccaggg ccggacgctg cgggatctct tgcgcgagcg cacccggctc
     3601 accccgcagg aggcactgtg gatccttgag ccggtgctcg cggcgctgtc ggccgcgcac
     3661 gccgctggcc tggtgcaccg cgacgtgaag cccgagaacg tcctgctcgc tgacgacggc
     3721 cgcgtgaagg tcgccgactt cgggctggcc agagccgccg ccaatctcga agccacgagc
     3781 gcgaccagcc tcatcggcac cgtcgcctat ttgtcgcccg agcaagtcat cagaggcatc
     3841 gccgacgaac gaagcgacgt ctattcggct ggcatcatgc tgtttgagat gctgaccggc
     3901 cggccgccct acgacggcga aaccgcggtt tcggtggcgt tgaagcatgc gcacgaggac
     3961 gtgccgccgc cgtccagttt ggtgtcgggc atccctacct ccgtcgacct gttggtggcc
     4021 agggcgacgg cgcgcgacgc cgaccggcgc ccgcacgatg ccggcgcgtt tctggcggag
     4081 gtcgtgcgcg tccgtggcgg cctggtcgca tccgacgcgg acgccgggag cctgacgagc
     4141 ctgatcaacc gcacgctcgt cgtcgaactc ccagacactg cgtccgtcgg accgaatccg
     4201 ccctggcagc tagggcaggc cccggttccg gcgcagccac catatggggg gcagcctggc
     4261 caaccggggc agccgccgcg gatcgtggag ccgccgcaac cgccggagga ggagtaccgc
     4321 cgaccacatc cacaccggaa tggttggatc gcgttcttca tcgtcctgct gcttgcagct
     4381 gctgcggccg gtgccgggtg gtggttcgcc gcgggccggt gggcgtccac gccgaatctg
     4441 gtcaacctgt cgtcatctgc cgccacggca aaagccaagg cggcccacgt gactgtgcag
     4501 gttggccagc cggagttctc gtccacggtc cccaagaact ccgtcgtgga gcagactccg
     4561 acggccaagc acaaggtgtt gcgcggcgac gcgatcacca tccgtctgtc gcaggggcca
     4621 cagctgttcc aagtgccgac gtacgacctc gctgggaata cgtcactggt cgactaccag
     4681 tcagaactca tcgctgcgca tctgaacgtc accgatcctc ccgttaccga atacagccca
     4741 accgtgattg ccgggaacgt gatttccgtc gatccgccgg tcggcacgaa cgagaaggcc
     4801 ggcacggcgg tgaccatcac cgtcagcctt gggccacagc ccatcccgat cgtcgactac
     4861 acaggccaac cggccgacca ggccacgcaa gctctgacga ccgccggctt caccgtggtg
     4921 ccgacccagg actacagcac ggcggtgaag gccggcttgg tcatctccca gtcgccgagc
     4981 agcgggtcag ggaagctcgg cgacaccgtc gcgctcgaca tctccaaagg cccgcagctg
     5041 ttcaaggtgc ctgacgtgac gagtcaactc aatggcgacc cgaaaaattg ggtcagcatc
     5101 cagcaagcga cgaagacctt gaccgatgca ggcttcaagg tcaacgtcgg gagcagcggc
     5161 cgattcggcg tcgtgaccag ccagagtccc aagcaacttt cgatgcagcc gctcggcacc
     5221 gtcatcacca tctacgccca ataagctcca cgaccggttc gccgtcccgg cggtctgaca
     5281 tccttgaccg atgccgcctc gcaagccgtc gccggtcggg gttcacgtcc cggtcgcggg
     5341 agggctgtcc agcggcggcc tcgcctacgc gcgcgatgtc ggcgccgagg cgatccaggt
     5401 ctttttgtcc aacccacg
//
LOCUS       DATCBJ010000633         4779 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_209525, whole genome shotgun sequence.
ACCESSION   DATCBJ010000633 DATCBJ010000000
VERSION     DATCBJ010000633.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 4779)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4779)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4779
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_209525"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..293
                     /locus_tag="VII50_12695"
     CDS             <1..293
                     /locus_tag="VII50_12695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009085513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEY5051752.1"
                     /translation="LAYGESPYGYLMTRRIERAMALLRRGDLSVTEVCFAVGCSSLGT
                     FSTRFTELVGVPPSTYRRQAARATAGMPSCVVKQVTRPIRNREAPVAQPHLS"
     gene            325..678
                     /locus_tag="VII50_12700"
     CDS             325..678
                     /locus_tag="VII50_12700"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY5051753.1"
                     /translation="MTSTQGIKTVLHPVSDLAAAKSVYAALLGVEPSSDAPYYVGYEV
                     EGQQIGLVPSGAQQGMTSPIAYWHVSDIEAKLAEVAAAGATVKESPRDVGGGRLVATF
                     TDPDGNVLGLLQDPS"
     gene            679..3036
                     /locus_tag="VII50_12705"
     CDS             679..3036
                     /locus_tag="VII50_12705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009085509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HEY5051754.1"
                     /translation="MTRHAADGHDLIRVHGARVNNLKDVSVVLPKRRLTVFTGVSGSG
                     KSSLVFGTIAADSQRLINETYSAFVQGFMPTLARPEVDVLEGLTTAIVVGQERIGGDA
                     RSTVGTATDANAMLRILFSRLGKPHIGSPQAFSFNVASISGAGAVTLQRAGRTTKERR
                     SFSILGGMCPRCEGRGSVSDFDLTALYDDGLSLNEGALLIPGYSMDGWFGRIFSGCGF
                     FDPDKPIKKFTKKELADLLYKEPTKIKVDGVNLTYEGLVPKIQKSMLSKDVDSLQPHV
                     RAFVERAIAFAVCPECGGTRLSEEARSSKINGKNIADVCAMQITDLAKWAQDLDEPSV
                     APLVASLRAILDSFVEIGLGYLSLERPSGTLSGGEGQRIKMIRHLGSSLTDVTYVFDE
                     PTIGLHPHDIERMNDLLLQLRDKGNTVLVVEHKPETIVIADHVVDLGPGAGAAGGEVV
                     FEGTVDGLRASGTQTGKHLDDRASVKRDVRARAGVLEVRGASLHNLRDVDVDIPRGVL
                     VVITGVAGSGKSSLIHGSVAGRDGVVVVDQAPIRGSRRSNPATYTGLLDPIRKAFAKA
                     SGVQPALFSSNSEGACPNCNGAGVIYTDLAMMAGVETVCEVCEGKRFQADVLDYKLGG
                     KDISEVLAMSVTEAQEFFGAGKAHTPAAHVILGRLADVGLGYLRLGQPLTTLSGGERQ
                     RLKLATHMAEKGDVYVLDEPTTGLHLADVEQLLGLLDRLVESGKSVIVVEHHQAVMAH
                     ADWIIDLGPGAGHDGGRIVFEGTPAELVADRSTLTGEHLAAYVGA"
     gene            3055..3465
                     /locus_tag="VII50_12710"
     CDS             3055..3465
                     /locus_tag="VII50_12710"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY5051755.1"
                     /translation="MDITINASFLPQDDPDAALAFYRDTLGFEVRNDVGYGGMRWITV
                     GPPNQPGTSIVLYPPGADPGITADERRTIAEMMAKGTYGIITFATHDLDETFAQLQAG
                     GAEVVQEPTEQPYGIRDCAFRDPAGNLIRVNQLR"
     gene            3468..4112
                     /locus_tag="VII50_12715"
     CDS             3468..4112
                     /locus_tag="VII50_12715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside/nucleotide kinase family protein"
                     /protein_id="HEY5051756.1"
                     /translation="MHTPAPEVARSDFAALVDRARQLAASPTRRILGITGAPGSGKST
                     LADEIVDALGADAVLVPMDGFHLAEAELKRLGIHDRKGALDTFDAGGFVALLRRMREN
                     TETVVYAPTFRRDLEEAIAGAIWVPSNVPLVVTEGNYLLRPDGAWSAVRGLLDEVWYL
                     EPDEHDRLDRLVRRHMDFGRDVDTANARARGSDQRNAEVIAATKEWADLIVVGS"
     gene            complement(4109..>4779)
                     /locus_tag="VII50_12720"
     CDS             complement(4109..>4779)
                     /locus_tag="VII50_12720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY5051757.1"
                     /translation="LVSGPLAGFLSDRFGARAFATAGMLVFGGSFVGLMLLPIDFPYW
                     TFALLITANGIGSGMFGAPNSSAIMSSVPAKQRGVASGMRATFQNSGTALSIGAFFSL
                     MIIGLASSLPKTLTNGLQQQGVSHAVAHQIASLPPVSSLFAAILGVNPIQHLLAPSGA
                     LASLPAANQQNLIGRTFFPNLISAPFHQGLVVVFAVAAALSLLAAVASVLRGERYVAS
                     DDLV"
BASE COUNT          883 a         1535 c         1592 g          769 t
ORIGIN      
        1 gactcgccta tggcgagtcg ccgtatggct acctgatgac aaggcgcatc gagcgggcga
       61 tggcgctgct gcgtcgcggc gacctcagcg tcacggaggt ctgtttcgcc gtcggctgct
      121 cgtcgttggg aacgttcagc actcgcttca ccgagctggt cggcgtccct cccagcacct
      181 accgtcgaca ggccgcacgc gcgacggccg ggatgccgtc gtgcgtggtc aagcaggtga
      241 ccagaccgat caggaatcga gaagcaccgg tcgcacagcc gcacctatcg tgatcacgaa
      301 ggtgaccacg acgaaggagt gcccatgact tcgacccaag gaatcaagac cgtgctgcat
      361 ccggtgtctg atctggcggc agccaagtcg gtgtatgccg ctctgctcgg cgtcgagccg
      421 tccagtgacg cgccctacta cgtcggctac gaggtcgagg gccagcagat cgggctggtg
      481 ccgagcggtg cacagcaggg catgacctcg ccgatcgcgt actggcacgt gtcggatatc
      541 gaggcgaagc tggccgaggt ggctgcggcg ggtgccaccg tgaaggagtc tccgcgcgac
      601 gtgggtggcg gccgcttggt ggccaccttc accgaccccg acggcaacgt cctcgggctg
      661 cttcaggatc cctcctgagt gacgcgtcac gctgccgatg gccacgattt gatccgcgtg
      721 cacggcgcgc gggtgaacaa cctcaaggac gtcagcgtcg tgctcccgaa gcgcaggctg
      781 acggtgttca ctggcgtctc cggctccggt aagagctcct tggtgttcgg cacgatcgcc
      841 gcggattcgc aacggctcat caacgagacg tacagcgcgt tcgtccaggg cttcatgccg
      901 acactggcac gaccggaggt cgacgtgctc gaggggctga cgacggcgat cgtcgtcggc
      961 caggagcgaa tcggcggcga cgcgcgctcg actgtcggca ctgcgaccga cgcgaacgcg
     1021 atgctgcgca ttctcttcag taggctcggg aagccgcaca tcggctctcc gcaggcgttc
     1081 tcgttcaacg tcgcgtcgat cagcggcgcc ggcgccgtga cactgcaacg cgctggtcgg
     1141 acgacgaagg aacgacgaag cttcagcatc ctcggcggca tgtgcccgcg gtgcgaaggc
     1201 cggggctcgg tctccgactt cgacttgact gcgctgtacg acgacggctt gtcgctcaac
     1261 gagggcgcgc tcctcatccc gggctacagc atggacggct ggttcgggcg gatctttagc
     1321 ggctgtggat tcttcgaccc tgacaagccg atcaagaaat tcaccaagaa ggaactggcc
     1381 gatctcctgt acaaggagcc gaccaagatc aaggtggacg gcgtcaacct gacctacgag
     1441 ggcctggtcc cgaagattca gaagtcgatg ctgtcgaagg acgtcgactc gttgcagccg
     1501 cacgttcgcg ccttcgtgga gcgggcgatc gcgtttgccg tctgtcccga gtgcggcggc
     1561 acccggctga gcgaggaggc gcggtcgtcg aagatcaacg ggaagaacat tgccgatgtc
     1621 tgcgcgatgc agatcaccga cttggcgaag tgggcgcagg atctagacga accgtcggtc
     1681 gcgccgctgg tggcgtcgct gcgagcgatc ctcgactcgt tcgtcgagat cggcctcggc
     1741 tacctcagcc tcgagcggcc atcgggcacg ctgtcgggcg gcgaggggca acgcatcaag
     1801 atgatccgcc atctcggctc atcgctgacc gacgtcacat acgtgttcga tgagccgacg
     1861 atcgggctgc acccccatga catcgagcga atgaacgacc tgctgctgca gctacgggac
     1921 aagggcaaca ccgtgctggt cgtcgagcac aagcccgaga ccatcgtgat cgccgaccat
     1981 gtcgtcgacc tcggcccggg cgcaggcgcc gccggcggcg aggtggtgtt cgaaggcacc
     2041 gtcgacgggc tgcgcgcgag cggcacgcag accgggaagc atctcgacga ccgagcgtcc
     2101 gtgaagcgag atgtgcgcgc gcgggcgggc gtgctcgagg tacgcggcgc cagcctccac
     2161 aacctgcggg atgtcgacgt cgacatcccg cgaggcgtgc tggtcgtgat tactggcgtg
     2221 gccgggtcgg gcaagagctc gctgatccac gggtcggttg ccgggcggga cggcgtggtg
     2281 gtggtcgacc aggcgccgat caggggctcg cgacggagta atccggcaac ctacaccgga
     2341 ctgcttgacc cgatccgcaa ggcattcgcg aaggccagcg gcgtgcagcc ggcgctgttc
     2401 agttccaact ccgagggcgc ttgccccaac tgcaacggcg ccggcgtcat ctacaccgac
     2461 ctcgcgatga tggccggtgt cgaaaccgtc tgcgaggtgt gcgaggggaa gcgcttccaa
     2521 gccgacgtgc tggattacaa attaggcggc aaggacatca gcgaggtgct cgcgatgtcg
     2581 gtgaccgagg cgcaggaatt cttcggcgcc ggtaaagcgc acacgccggc tgcgcacgtc
     2641 atcctcggcc ggctcgccga cgtcgggctt ggctacctgc gcctcgggca gccgcttaca
     2701 accctctccg gtggcgaacg gcagcgactg aagctggcca ctcacatggc ggaaaagggc
     2761 gacgtctacg tcctcgacga gcccaccacc ggcctgcacc tcgccgacgt cgagcagctg
     2821 ctcggcctgc tcgaccggct cgtcgaatcc ggcaagtcag ttattgtcgt cgagcaccac
     2881 caggcggtca tggcgcacgc ggactggatc atcgacctcg gccccggcgc cgggcacgac
     2941 ggtggccgca ttgtcttcga gggcaccccc gctgagctcg tcgccgaccg ctcaaccctc
     3001 accggcgagc acctcgcggc ctacgtcggc gcctgacccc agaaagggac tgtcatggac
     3061 atcacgatta acgcgagctt cctcccgcaa gacgaccccg acgcggctct cgccttctat
     3121 cgcgacacgc tgggttttga ggtcagaaac gacgtcggat acggcggcat gcgctggatc
     3181 acggtcggcc cacccaatca gccgggcacg tccatcgtgt tgtacccgcc gggcgccgat
     3241 cctggcatca ccgccgacga gcgccgcacc atcgccgaga tgatggccaa ggggacctac
     3301 ggcatcatca ccttcgccac ccacgacctc gacgagacct tcgcccaact gcaggcgggc
     3361 ggtgccgaag tcgtccagga gccgacggag cagccgtacg gcattcgcga ctgcgcgttc
     3421 cgagatcccg caggcaatct gatccgcgtc aaccagcttc gctgacagtg cacacgcccg
     3481 cgccggaggt agcgcgttcc gacttcgcgg cactagtcga tagggcccga caactcgcag
     3541 cttcaccgac ccggcggatt ctcgggatca ccggggcgcc cggttcgggc aaatcgacgt
     3601 tggccgacga gatcgtcgac gcgcttggcg ccgatgcggt gctcgtgccg atggatggat
     3661 tccatctcgc tgaagccgag ctcaagcggc tcggcatcca cgaccgcaag ggcgcgctcg
     3721 acactttcga cgccgggggc ttcgttgccc tgcttcgccg aatgcgcgaa aacaccgaga
     3781 cggtcgtgta cgcacccacg tttcgccgcg accttgagga ggcgatcgcc ggtgcaatct
     3841 gggtgccgag caacgtgccc cttgttgtca ctgaaggcaa ctacctctta agaccggacg
     3901 gcgcgtggtc ggcggttcgt ggattgctcg acgaggtttg gtacttggaa ccagacgagc
     3961 acgatcggtt ggatcgattg gtcagacggc acatggactt cggtcgtgac gtcgacaccg
     4021 cgaacgcacg agcacgaggc agcgatcaac gaaacgccga ggtgatcgca gcaacgaagg
     4081 agtgggcgga tctcatcgtg gtcggcagct agaccaaatc gtctgacgcg acgtagcgct
     4141 cgccacgcag gaccgacgcg acagccgcga gcagcgacag cgctgcggcg accgcgaaca
     4201 cgacgacgag accctgatga aagggcgccg atatcaagtt ggggaagaac gtgcggccaa
     4261 tcaggttctg ctggttcgcc gccggcaagg atgcgagcgc accgcttggg gcgagcaggt
     4321 gttggatcgg gttcacccca agaatcgctg cgaataagga ggaaaccggc ggcaggctgg
     4381 cgatttgatg tgcgacggca tgcgacacgc cctgttgttg caatccgttc gtcagcgtct
     4441 tcgggaggct actcgccagg ccgataatca tcagcgagaa gaaggcaccg atactcaatg
     4501 ccgtgccgga attctggaac gtcgctcgca tgccggacgc cacgccgcgc tgcttggcgg
     4561 gaacactgct catgatcgcc gaagagttcg gcgcgccgaa catgccggac ccgatgccat
     4621 tggcggtgat gagcaacgcg aacgtccagt agggaaagtc gatgggcagc aacatcagtc
     4681 cgacgaagct tccgccgaag acgagcatgc ccgcggtcgc gaaagctcga gcgccgaacc
     4741 ggtcggagag gaatcccgcc agtggcccgg acacgagga
//
LOCUS       DATCBJ010000634         4008 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_83658, whole genome shotgun sequence.
ACCESSION   DATCBJ010000634 DATCBJ010000000
VERSION     DATCBJ010000634.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 4008)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4008)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4008
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_83658"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(107..361)
                     /gene="rpmA"
                     /locus_tag="VII50_12725"
     CDS             complement(107..361)
                     /gene="rpmA"
                     /locus_tag="VII50_12725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007492765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HEY5051758.1"
                     /translation="MAHKKGASSSRNGRDSNAQFLGVKRYGGQLVNAGEILIRQRGTH
                     HHPGLNVGRGKDDTLFALAAGRVEFGTARGRRVVNIVPGT"
     gene            complement(371..685)
                     /gene="rplU"
                     /locus_tag="VII50_12730"
     CDS             complement(371..685)
                     /gene="rplU"
                     /locus_tag="VII50_12730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019513235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HEY5051759.1"
                     /translation="MYAIVRSGGRQHKVAVGDVVEVDRVDGEPGTSVTLAALLLVDGD
                     AVTSDAKALGNVTVTAEVVDHTKGPKIHILKYKNKTGYRKRQGHRQRHSRLKVTGIET
                     GK"
     gene            complement(820..1179)
                     /locus_tag="VII50_12735"
     CDS             complement(820..1179)
                     /locus_tag="VII50_12735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5051760.1"
                     /translation="MNVAPELSPGSPAPGPAAVFGDALADALGAWLGDAGLVDVAAAG
                     ADVAAAAPTAVEPAVDGAADVLAEVAAAVEPGADGPADLVVAVHPAVTRAGTSTSAAA
                     IRRIPPSSRILRRWGPV"
     gene            1205..2239
                     /locus_tag="VII50_12740"
     CDS             1205..2239
                     /locus_tag="VII50_12740"
                     /inference="COORDINATES: protein motif:HMM:NF014312.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncE family protein"
                     /protein_id="HEY5051761.1"
                     /translation="MPVDQIAPAARMVLVKTITGHLDEKSVVSSQTGLFFAQNMMYLH
                     TMNVYDRNFHLVKIIPDAVNLASLGYPQYPGTYKGAPVEAAFTPDHKYVYVSNYSMYG
                     AAPFTREGSDTCTLADHYPDSFVYRVNLVTLTIDQAIEVGAVPKFVAVTPNGKYLLVS
                     NWCSYSESIIDTATGNVVTTIPLGPYPRGITVDPVSGTAYVAVMGSTRIAAINLETFA
                     LSWISNVGSAPRHLVMSADGHWLYATINGEGVVDKISLITRTVVAKIATGRAPRSMTI
                     SPDGSYIYVVNYMSNTLSKVRTSDMHIVQTIATNPDPIGITYDNTTHRVWVACYSGSI
                     MVFQDGTPTS"
     gene            complement(2242..>4008)
                     /locus_tag="VII50_12745"
     CDS             complement(2242..>4008)
                     /locus_tag="VII50_12745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006549480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HEY5051762.1"
                     /translation="TRLEAKKQRRREGREQGRGHGRRGLVITESEFLARRESVDRVMA
                     VRQRGDRTQIGVLEDGILVEHFVSTASTNRMVGNVYLGRVQNVLSGMEAAFVDIGKGR
                     NAVLYAGEVNFDTSGLEGGPRRIESALKPGQAVVVQVTKDPIGQKGARLTSQISLPGR
                     YLVYVPEGSMMGISRKLPDVERSRLKAILKKVTPEGAGVIVRTAAEGASEEDLTRDVA
                     RLAAQWEVIQKKSETANAPALLYSEPDLTIRVIRDVFNEDFNQLVVSADSDWDTVEAY
                     VAHVAPDLVPRLSQWTSEEDIFTHYRVDEQIAKALDRKVWLPSGGYLVIDRTEAMIVI
                     DVNTGRFIGKGGNLEETVTKNNLEAAEEIVRQLRLRDIGGIIVIDFIDMVLEGNRDLV
                     LRRLVECLGRDRTKHQVAEVTSLGLVQMTRKRVGNGLLEAFSEVCEVCNGRGVIVHTD
                     LVEHNHAPRQLTVPKPSPARVAAAKGKSSRGRGGQGGAATSTDGEVAAAEPTTEAVAD
                     VGGASDAEHSNADGNAHPHPEAAEPTNSDHANSEDAEAPAAKKSSGASRSRRGGRRRG
                     GSKSNAGADHTAAAHDSGPAGA"
BASE COUNT          661 a         1352 c         1304 g          691 t
ORIGIN      
        1 tcggcatcgt gtcctccttc gcagttgcaa ctacacggtg gaaacaagac gagggcggac
       61 cgaaaccggt tcgccctcgt gtactgctgc ggtgcaatga ccgggcttag gtccccggga
      121 cgatgttgac cacgcggcgg ccgcgcgcgg tgccgaactc gacccgaccc gccgccaggg
      181 cgaacagcgt gtcgtccttg ccccgcccga cgttcaggcc ggggtgatgg tgggtgccgc
      241 gctggcgaat gaggatctcg ccggcgttca ccagctggcc gccgtagcgc ttcacgccga
      301 ggaactgggc attggagtcg cggccgttac ggctggacga tgcgcccttc ttgtgagcca
      361 tgtcagcagc ctactttccg gtctcgatgc cggtgacctt caaacggctg tggcgctgac
      421 ggtggccctg ccgcttgcgg tatccggtct tgttcttgta cttgaggatg tggatcttgg
      481 ggcccttggt gtggtcgacg acctcggccg tcacggtcac gttgcccagg gccttcgcgt
      541 cgctggtcac cgcgtcaccg tcgacgagca ggagcgcggc gagagtgacc gatgtgccgg
      601 gctcgccatc gacccggtcg acctcgacga cgtcgccgac ggccaccttg tgctgcctgc
      661 cgccgctgcg aacgatcgcg tacaccgcgg aactcctgcc tgctcgaacc tgacgactcg
      721 gactcaccgc ggacacgcga caccgacaac ggacgcgcgg cgactggcgc attctcaagc
      781 gcgccgaccc ccaagagtac ggaccagcgc gccagggggt caaaccgggc cccatcggcg
      841 aagaatccgc gaggatggcg ggatgcgacg gatagcggct gcgctggtgc tcgtgcctgc
      901 cctggtgacg gccgggtgca cggcgacgac caggtcggcc gggccgtcgg cgccgggttc
      961 gacggctgcg gctacctcgg cgagcacgtc cgcggcgcca tcaaccgcgg gttcgaccgc
     1021 cgttggcgcg gcggcggcaa catccgcgcc cgcggccgcc acgtcaacca gtccggcgtc
     1081 ccccagccag gccccgagcg cgtcggcgag tgcgtcgccg aacaccgcag cggggccggg
     1141 cgcgggcgaa ccgggtgaca actcgggcgc cacgttcaag ggcgccggcc cctcggcgtc
     1201 cgccgtgcct gtcgatcaga tcgcaccggc ggcgaggatg gtcctggtca agaccatcac
     1261 gggtcacctc gacgagaagt cggtggtgtc gtcgcagacg ggactcttct tcgcgcagaa
     1321 catgatgtat ctgcacacga tgaacgtgta cgaccgcaac ttccacctgg tgaagatcat
     1381 cccggacgcc gtgaacttgg cgagcctcgg ctatccccag tacccgggca cctacaaggg
     1441 cgctcccgtc gaggcggcgt tcactcccga ccacaagtac gtgtacgtgt cgaactattc
     1501 gatgtacggc gcggcaccgt tcacccgaga gggttccgac acctgcacgc tcgccgacca
     1561 ctaccccgac agcttcgtct accgagtgaa cctcgtgacg ctgacgatcg accaggcgat
     1621 cgaggtcggc gcggttccga agttcgtcgc ggtcacgccg aacggcaagt acctgctggt
     1681 gtcgaactgg tgctcctaca gcgaaagcat catcgatacc gcgaccggca acgtcgtcac
     1741 aacgatcccg ctcggcccct acccgcgcgg catcacggtc gatccggtca gcggcaccgc
     1801 ctacgtcgcc gtcatgggca gcacgcggat cgcggccatc aacctcgaga cgttcgcgct
     1861 cagctggatc tcgaacgtcg gctcagcacc gcggcatctg gtgatgagcg cggacggtca
     1921 ttggctgtac gccacgatca acggtgaggg cgtcgtcgac aagatctccc tcatcacccg
     1981 gacggtagtg gccaagatcg cgaccggtcg ggcgccgcgc agcatgacga tctcgccgga
     2041 tggcagctac atctacgtcg tgaactacat gtccaacacg ctgagcaagg tgcgcacgag
     2101 cgacatgcac atcgtgcaga ccattgcgac caatcccgac ccgatcggca tcacctacga
     2161 caacacgacg caccgggtgt gggtcgcgtg ctacagcggc tcgatcatgg tgttccaaga
     2221 cgggacgccg accagctgag gttaggcgcc ggccggtccg gagtcgtggg ctgctgcggt
     2281 gtgatccgcg ccggcgttcg acttcgaccc accccgacga cgaccgccgc gacgtgaccg
     2341 tgacgcaccc gacgacttct tggcagccgg cgcctccgcg tcctccgagt tcgcgtggtc
     2401 ggaattcgtg ggctcggcgg cctcggggtg cgggtgcgcg ttgccgtcgg cgttcgagtg
     2461 ctcggcgtca ctagcgccgc caacatccgc gacggcttcg gtggtcggct cggcagccgc
     2521 gacctcaccg tcggtcgacg ttgccgcgcc accctggccg ccgcgcccgc gtgagctctt
     2581 gcccttcgca gccgcgaccc gtgcggggct cggcttgggg acagtgagct ggcgtggcgc
     2641 gtggttgtgc tcgaccagat cggtgtgcac gatgacgccg cgtccgttgc agacttcgca
     2701 gacttccgag aacgcctcga gcagaccgtt gccgacgcgc ttgcgggtca tctgcacgag
     2761 accgagcgag gtgacctcag cgacctgatg cttcgtgcga tcgcgaccga gacactcgac
     2821 gagtcggcgc aacaccaggt ctctgttgcc ttcgaggacc atgtcgatga agtcgatgac
     2881 gatgatgccg ccgatgtcac gcaatcgaag ctgacggacg atttcttcgg ccgcctcgag
     2941 attgttcttg gtgaccgtct cttcgaggtt gccacccttg ccgatgaacc ggccggtgtt
     3001 gacgtcgatc acgatcatgg cttcagtgcg atcgatgacg aggtagccac cgctgggcaa
     3061 ccagaccttg cggtcgaggg ccttcgcgat ctgctcgtca acgcgatagt gggtgaagat
     3121 gtcttcttcc gacgtccact gcgagagccg cggcaccagg tcgggagcga cgtgggcgac
     3181 gtacgcctcg acggtgtccc agtctgaatc cgcagacacg acgagctgat tgaaatcttc
     3241 gttgaagacg tcgcggatga cccggatcgt cagatcgggc tcgctgtaca gcagcgcagg
     3301 ggcgtttgcg gtctcagact tcttctggat gacttcccat tgcgccgcaa gtcgcgcgac
     3361 gtcacgcgtg aggtcctcct cggaggcacc ctcggccgcc gtgcgcacga tgacgccggc
     3421 gccttcaggt gtgaccttct tcaggatcgc cttcaaccga gaccgttcga cgtccggcag
     3481 tttgcggctg atacccatca tcgaaccctc gggcacgtac acgaggtagc gaccggggag
     3541 gctgatctgg ctggtcaggc gggcaccctt ctgcccgatc ggatccttcg tgacctggac
     3601 gacgacagct tggcctggct tcaacgccga ctcgatgcga cgcggaccac cttcaaggcc
     3661 ggaggtgtcg aagttcacct caccggcgta tagaaccgcg ttgcggccct tgccgatgtc
     3721 gacgaacgcc gcctccatgc cggacagcac gttctgcacg cggccgagat agacgttgcc
     3781 gaccatgcga ttggtcgagg ccgtgctgac gaagtgctcg accagaatgc cgtcttcgag
     3841 gacaccgatc tgagtgcgat cgccgcgctg ccgaactgcc atgacacggt cgaccgactc
     3901 ccggcgggca aggaactcgg actcggtgat gaccagaccg cgccgcccgt gccctctgcc
     3961 ctgctcgcga ccttcgcgac ggcgctgctt cttggcctcg aggcgggt
//
LOCUS       DATCBJ010000635         2798 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_425495, whole genome shotgun sequence.
ACCESSION   DATCBJ010000635 DATCBJ010000000
VERSION     DATCBJ010000635.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2798)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2798)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2798
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_425495"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(158..1540)
                     /gene="radA"
                     /locus_tag="VII50_12750"
     CDS             complement(158..1540)
                     /gene="radA"
                     /locus_tag="VII50_12750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009084198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HEY5051763.1"
                     /translation="MPKSTTSGNARPSRHAFRCGECGWESLKWVGRCGECQAWGTIEQ
                     PGLPRLRSVAATSVTEAARPIGEIDAESARSQPTGVDELDRVLGGGLVPGVVVLLAGE
                     PGVGKSTLVLEVASKFATPGTRTLYITGEESTAQVRLRAGRIKALSPDLFLAAETDIG
                     AVLKHLDDVGPSLLIVDSVQTMAIADVDGSAGGVTQVRQVAAALIAVAKERGIATVLV
                     GHVTKDGSIAGPRVLEHLVDVVLQFEGERHSRLRLVRAIKNRYGACDEVGCFDLTEEG
                     IVGLADPSGLFLSRRSAPVSGTCITVALEGRRPLVAEVQALVAKSNMAIPRRTTSGLD
                     AGRVSMVLAVTERRAKILLTGADVFTATVGGVKLTEPAGDLAVALAVASAENDKALLP
                     GLVAIGEVGLAGEVRRVEGVGRRLIEAARLGFTHALVPPDSGPVPPGVHSIEVSDLAQ
                     AIEYALATNG"
     gene            complement(1604..2455)
                     /locus_tag="VII50_12755"
     CDS             complement(1604..2455)
                     /locus_tag="VII50_12755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5051764.1"
                     /translation="MPSKPPQQSTTSGRAEPAHPTIPTLPIPRNASAGERTPVPETGL
                     DFAREWVEFVDPADAEQIFRCDLTWLTSSWGCIFGNGCHGIVPGRAADGCCSHGAFYS
                     GRADEKRVRKFAEQLTPESWQYYKKGQKGFTELEDGKRRTRRVDGACIFLNRPGFEGG
                     AGCSLHSLALTIDRHPLETKPDVCWQLPIRRTFDWVDRPDETKVLIVTIGEYDRRGWG
                     TGGHDLTWWCTSSPEAHGARDRVYVSYGPELTELMGLQGYAELVAHCERRIASKTPVA
                     PHPADPQ"
     gene            2543..>2798
                     /locus_tag="VII50_12760"
     CDS             2543..>2798
                     /locus_tag="VII50_12760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014155562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5051765.1"
                     /translation="MGTAEGAALERATTGPTVALSTSSVWPESTAVAFEVAARLGYDG
                     VELMVSGDPVSQDLDTVRRLIDYHQMPVVSVHSPCLLISQR"
BASE COUNT          455 a          920 c          929 g          494 t
ORIGIN      
        1 cgccggtgtt gccgcgcagg atccgttcga gtgcatcgcg caggtcggta ccaggcgcga
       61 tcgtcgcgag agttgcccgc agtttctcgt cgacggtccg gtcaaaccga tcgttggctg
      121 gcaccgggct cccctgttgt acggcgttac ggcgattcta gccgttggtc gcgagcgcgt
      181 actcaatcgc ttgtgcaagg tcggacacct caatcgaatg gacgccgggg ggtaccggtc
      241 ctgagtcggg tggcaccagt gcgtgagtga acccgagccg ggcggcttct atgaggcggc
      301 gccctacccc ctcgacgcga cgcacctcgc cggctagacc gacctcacct atcgcaacga
      361 gacctggaag cagtgctttg tcgttctcgg cactcgcgac ggcgagtgcg acagcgaggt
      421 cccccgcagg ctcggtcagc ttcaccccgc cgacggtggc agtgaagaca tcagccccgg
      481 tgagcaggat cttggctcgc cgctctgtta ccgccagcac catcgatacc cggccggcgt
      541 cgagccccga ggtcgtgcgc cgcggtatcg ccatgttcga cttggcgacc aacgcctgca
      601 cctctgcgac caacgggcgg cgaccctcca aggccaccgt gatgcaggtg ccggacaccg
      661 gagcactgcg tcgcgacaag aacaggccac tgggatcagc gagcccgacg atgccctcct
      721 cggtgaggtc gaagcacccg acctcgtcgc acgcgccgta gcggttcttt attgctcgaa
      781 ccaaccggag ccgtgaatgc cgctcgccct cgaactgcag cacgacgtcg acgaggtgtt
      841 cgagcacgcg agggcctgcg atcgacccgt ctttggtgac gtggccgaca aggacggttg
      901 cgatgccgcg ctctttggcg acggcgatga gtgcggccgc cacctggcgg acctgcgtca
      961 ccccaccggc cgacccatcg acgtccgcga tggccatagt ttgcaccgag tcgacgatca
     1021 gcaggctagg gccgacgtcg tcgagatgtt tcaagactgc gccgatgtcc gtttccgctg
     1081 ccagaaagag gtcgggcgac agtgccttga tgcgccccgc gcgcagccgc acctgtgcgg
     1141 tcgactcctc gccggtgatg tagagcgtgc gggtgccggg agtggcgaac ttcgaggcga
     1201 cctctaggac caacgtcgac ttgcccacac ccggctcgcc agccaacagc accacgacgc
     1261 cgggtactag gccgccgccg agcactcggt caagctcatc gacaccggtc ggctgggaac
     1321 gcgccgattc ggcatcgatc tcaccgatgg ggcgcgcagc ctcagtgacc gacgtcgcgg
     1381 caacactgcg aagtcgaggc aagccgggtt gctcgatggt gccccacgcc tggcattcac
     1441 cgcagcggcc gacccacttc aaagactccc aaccgcactc accgcaccgg aatgcgtgcc
     1501 tggaaggtcg ggcattgccg gatgtggtgc tcttgggcat gcccagcagg ctagtgccgc
     1561 gcgcggacag ttccctggat cgcgaggcag gttgggggtt tgattattgg gggtcggccg
     1621 ggtgcggcgc gaccggtgtc ttcgaggcga ttcggcgctc gcagtgcgcg accaactccg
     1681 cgtaaccctg gaggcccatc aactcggtca gttcgggacc gtaggagacg tagacccgat
     1741 cacgcgcgcc gtgtgcttcg ggtgagctgg tgcaccacca cgtgaggtcg tgtccgcccg
     1801 ttccccatcc cctgcggtcg tattcaccga tcgtgacgat caataccttg gtttcgtcgg
     1861 gtcggtcgac ccagtcgaac gtgcggcgaa tgggcagctg ccagcacaca tcgggtttgg
     1921 tctcgagcgg gtgccggtcg atcgtcaacg cgaggctgtg caatgagcag ccggcgccgc
     1981 cctcgaatcc cggccggttc aagaaaatgc atgcaccgtc gaccctgcga gtgcgtcgct
     2041 tcccgtcctc gagttcggtg aagcccttct ggcccttctt gtagtactgc caagattccg
     2101 gcgtgagttg ctcggcgaac ttgcggacgc gcttctcgtc ggcgcgtccg gagtagaacg
     2161 caccatggct gcagcagccg tccgcggcac gaccgggcac gatgccgtga cagccgttgc
     2221 cgaagatgca tccccagctc gacgtcaacc acgtcaggtc gcagcgaaag atttgctcgg
     2281 cgtcggcggg gtcgacgaac tcgacccact cgcgcgcgaa gtcgagaccc gtttccggca
     2341 ccggtgtgcg ttcaccggcg gacgcgttac gtggtattgg gagcgtcggg atcgtcgggt
     2401 gggcgggttc ggctcgaccg gaggtcgtgg actgctgcgg tggcttgctt ggcatgcgca
     2461 aagactaggc cccaaggcat cagagtcgga cgccgatgtg caccgtccgg tgtgtcggct
     2521 ggtgtcgtcg taggctcggg gaatgggcac tgcggaagga gcggcactgg agcgggcgac
     2581 aactggcccg acggtggccc tttcgacgtc gtccgtgtgg cccgaatcga ctgctgtcgc
     2641 cttcgaggtc gcggctcgac tgggctacga cggcgtcgag ttgatggtct ccggcgatcc
     2701 cgtcagtcaa gacctcgaca ccgtgcgtcg gctcatcgac taccaccaga tgccggtcgt
     2761 ctccgtgcat tcgccctgcc tgctgatcag ccagcggg
//
LOCUS       DATCBJ010000636         2500 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_452471, whole genome shotgun sequence.
ACCESSION   DATCBJ010000636 DATCBJ010000000
VERSION     DATCBJ010000636.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2500)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2500)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2500
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_452471"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(109..756)
                     /locus_tag="VII50_12765"
     CDS             complement(109..756)
                     /locus_tag="VII50_12765"
                     /inference="COORDINATES: protein motif:HMM:NF024949.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEY5051766.1"
                     /translation="MCEPRPDDPERVAAQLVQQARATTHLSQRALAALAGVPQSTVAR
                     IEGGHMQPTLPLLYRILQAAGLKARTVLEPAADAVIGPPAALPAAPAYRPMTLVDLAG
                     HLAGADDDQLRWRLIAEFLEEHRHEPIDVRAALLRGEPPATGDERWDVFVAALAEHLA
                     GLDDRGTAAWTAQRRLDVFWFPFNTAAARVDAYVHAPAGFRRRGIFVAPQELGVA"
     gene            903..1817
                     /locus_tag="VII50_12770"
     CDS             903..1817
                     /locus_tag="VII50_12770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009086035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY5051767.1"
                     /translation="MEQIAIRTVGLSKRYGDVDALNDLDLEVAPGEVLGYLGPNGAGK
                     TTTIRLLLGLIRPTRGRAEIFGFDCQRQPVEAHRRMAYVAGEASLWPSLTGAETLHLL
                     ARVQGQVDVAYRDELVERFALDPSKKVRTYSKGNRQKVILIAALMARPDLLVLDEPTS
                     GLDPLMEQAFRHCVQEAKERGQTVFLSSHILSEVEALCDRIGILRAGKLVEMGTLAEM
                     RHLSSLTVEATFDASVPDLSHVLGVSSIQVEGRVVRCQVRGTVGPLLEVLAGAGVHEL
                     LSREPSLEELFLAHYGSSDDTADAAHVG"
     gene            1807..>2500
                     /locus_tag="VII50_12775"
     CDS             1807..>2500
                     /locus_tag="VII50_12775"
                     /inference="COORDINATES: protein motif:HMM:NF024091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEY5051768.1"
                     /translation="MSADLALRVPLATMRRPGLVAATLTARRAARSAVVWGCVFGLYV
                     TSSALGYAATYKTALARAQLAATFGTNAGFNALIGPAHGIGTVAGFTAWRSLGVLSIV
                     GGVWGLLAGTRLLRGEEDAGRWELLLVGHTTRRGATARALAGLAVGLLTLWGVTAVIT
                     VAVGRAHTVNIAVGPSLFLALALVCSAAIFLTLGALASQLAPTRRQAAGYAAAVLGIS
                     YALRMIADSGTGL"
BASE COUNT          386 a          758 c          890 g          466 t
ORIGIN      
        1 cgatgacgta gatgtctccg acgacaccgc gtcgctgtag gcgttcgcca agtcgcgtga
       61 acagatcgcg gatgcgagcg cggtcgaaca gcggcccatc ctctggcgtc acgcgacgcc
      121 taactcttgc ggtgcgacga agatgcctcg gcggcggaaa cccgcgggtg catggacata
      181 cgcgtcgact cgagcagccg cggtgttgaa cgggaaccag aagacgtcca gtcggcgctg
      241 cgcagtccag gccgcggtcc cacgatcgtc aaggccagca aggtgttcag ccaacgcggc
      301 aacgaacaca tcccaccgtt cgtcgccggt ggccggaggc tctccgcgga ggagcgcggc
      361 ccgcacgtcg attggctcgt gccgatgctc ctccaagaac tccgcgataa gccgccaccg
      421 caactgatcg tcgtccgcgc ctgcgaggtg ccccgcaagg tcgacgagcg tcatcggccg
      481 gtacgcggga gccgccggaa gtgctgcggg ggggccgatc acggcatcgg ctgcgggctc
      541 cagcacggtg cgcgccttca gtcccgcagc ttggaggatt cggtagagca gcggaagcgt
      601 gggctgcatg tgtccgccct cgatccgcgc caccgtcgac tgcggtacgc cggccaaggc
      661 tgcgagagcc cgttgagaaa ggtgcgttgt ggcgcgggcc tgctgcacca gctgagccgc
      721 aacacgttcg gggtcatccg gacggggctc acacaccgct gccatctcgc aatgatagct
      781 gatctgcagt cactcgggct cacgctcgag attgcgtttg tgcaggcggt cgggcaggtc
      841 ttttggccct gtggtgattc cagttctttc agtaatctct gaaagaacct tggaggtcac
      901 tggtggagca gatcgcgatt cggacggtcg gactgtcgaa gcgttacggc gatgtcgatg
      961 cgctcaacga tcttgacctg gaggtggcac cgggtgaggt gttgggctac ctcggtccga
     1021 acggtgccgg gaagacgacg acgatccggt tgttgcttgg gttgatccgg ccaacgcgtg
     1081 ggcgcgcgga gattttcggt ttcgattgcc aacggcagcc cgtcgaggcc catcgccgta
     1141 tggcgtatgt cgccggggag gcgagtctgt ggccgtcgtt gaccggtgcg gagactctgc
     1201 atcttttggc acgggtgcag ggacaggtcg atgtcgccta ccgcgacgaa ctcgtggagc
     1261 ggttcgccct ggatccgtcg aagaaggtgc ggacctactc caaaggaaat cgacagaagg
     1321 tcatcttgat cgcggctctc atggcgcgcc cggatctcct tgtgctggat gagccgacga
     1381 gcgggttaga tccgctgatg gagcaggcgt ttcgtcattg cgtccaggaa gcgaaagagc
     1441 gcggccagac ggtgttcctg tcgtcgcaca tcttgagcga ggtcgaggcg ttgtgcgatc
     1501 ggatcggcat tctgcgcgcc gggaagcttg tcgagatggg cacgctggcc gagatgcgcc
     1561 acctgtcctc gctgaccgtg gaggcgactt tcgacgcctc agtgccggat ctgtcgcacg
     1621 tcttgggagt cagttcgatt caggtcgagg gccgagttgt tcgttgtcag gttcgcggga
     1681 cggtcgggcc gctgcttgaa gtgttggcgg gcgcgggggt gcacgagtta ctgagtcgcg
     1741 aaccgtcgct ggaggagctg ttcttggccc actacggcag cagcgatgac acggcggatg
     1801 ccgctcatgt cggctgacct cgcgctgcgg gttcccctcg caacgatgcg ccggccaggg
     1861 ctggtcgcag cgacactgac ggcacgcagg gcagcgcgtt cggctgtcgt gtggggatgt
     1921 gtgttcggcc tgtatgtcac gtcgtcggca ctcggttatg cggcgactta taagacagcc
     1981 ttggcgcggg cgcagttggc ggcgacgttc ggcacgaacg ccggcttcaa cgccctcatc
     2041 ggacctgcgc acgggatcgg gacggtcgcc ggttttactg cctggcgcag cctcggtgtg
     2101 ctgagcatcg tcggtggcgt gtggggactg ctggccggaa ccaggctgct acgcggagaa
     2161 gaggacgccg ggcgctggga gctgctgctt gtcgggcaca ccactcgccg cggcgcgact
     2221 gcacgggcgc tggccgggct cgccgtcggg ctcctgaccc tgtggggagt gacggcggtc
     2281 atcaccgtcg cggtcggccg agcccacacc gtgaacatcg cggtcggccc atcgctgttc
     2341 ctcgcccttg cactcgtgtg cagcgcggcg atctttctca ccctcggggc gttggccagc
     2401 cagctggcgc caacgcgtcg tcaagccgcc gggtacgccg ccgccgtgct cgggatttcg
     2461 tatgcgctgc ggatgatcgc cgactcgggc accgggctgg
//
LOCUS       DATCBJ010000637         2565 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidothermaceae bacterium isolate SMAG_U7669
            k141_99173, whole genome shotgun sequence.
ACCESSION   DATCBJ010000637 DATCBJ010000000
VERSION     DATCBJ010000637.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962045
            Sequence Read Archive: SRR3985388
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidothermaceae bacterium (soil metagenome)
  ORGANISM  Acidothermaceae bacterium
            Bacteria; Actinomycetota; Actinomycetes; Acidothermales;
            Acidothermaceae.
REFERENCE   1  (bases 1 to 2565)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2565)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 13:04:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,558
            CDSs (total)                      :: 2,542
            Genes (coding)                    :: 2,520
            CDSs (with protein)               :: 2,520
            Genes (RNA)                       :: 16
            tRNAs                             :: 15
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 18 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 1 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2565
                     /organism="Acidothermaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_99173"
                     /isolate="SMAG_U7669"
                     /isolation_source="soil"
                     /db_xref="taxon:2575569"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1005)
                     /locus_tag="VII50_12780"
     CDS             complement(<1..1005)
                     /locus_tag="VII50_12780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HEY5051769.1"
                     /translation="MARTHDAASTATDDSGVAVSGAAVSVEGATVNEPVHDLAALRAE
                     RTAQADAWLRELLGGADGVALVAVGGYGRGELGPGSDLDVMMLHDGRSDVVQIADQVW
                     YPIWDAGWQLDHSVRTVADAVSVANADMKAALGMLHARHIAGVPDLTARLREHAFGQW
                     RSRASKRLPELHEMVRDRAERLGEVAFLLEPDLKEARGGLRDVHALEAIAAAWVAAGP
                     GPPVRAAYRRLIDVRDTLQEVAGRPTTRMVAQEQEAIAARLGMADADELLRAISEAAR
                     TVSYASDVTWRSVEGSLRRRPKAERRPVAEGVVEQDGEVVLARGVDPTPDPTLVVRAA
                     A"
     gene            complement(1035..1373)
                     /locus_tag="VII50_12785"
     CDS             complement(1035..1373)
                     /locus_tag="VII50_12785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018850023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HEY5051770.1"
                     /translation="MRLITAIVKPFKLDDVKSALETFGVTGMTVSEANGYGRQRGHTE
                     VYRGAEYEVDLVPKVRIEVLVDEGDADDVIDVIVKSAQTGKIGDGKVWSTPVENVVRV
                     RTGERGNSAL"
     gene            complement(1522..>2565)
                     /gene="ftsY"
                     /locus_tag="VII50_12790"
     CDS             complement(1522..>2565)
                     /gene="ftsY"
                     /locus_tag="VII50_12790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HEY5051771.1"
                     /translation="PRQSAPPLRPAPNAASVSAAAEPAVDTEAVLEPDLTETPEPAAG
                     RLVRLRARLARSESTLGRGLLALLSRDTLDDETWQEVEDLLLTADVGVSASNELVDSL
                     RTKAKVLGTRSAADARAFLREGLLELVDPQLDRSLATQRPAGEGPAIVLVVGVNGTGK
                     TTTCGKIARLLVAEGRTVVLGAADTFRAAAADQLETWGRRVGAQTVRGPEGGDPAAVA
                     FDAVKTGIELGVDTVLVDTAGRLHTKAGLMDELGKVKRVIEKQTPVDEVLLVLDATTG
                     QNGLVQARVFAEVVDITGVVLTKLDGTAKGGIVISVQRELGVPVKLVGLGEGADDLAP
                     FEPEAFVDALLGS"
BASE COUNT          421 a          890 c          803 g          451 t
ORIGIN      
        1 agcagcagcg cgcacgacaa gagtcggatc aggagtcggg tcgactccgc gcgcgagcac
       61 cacctcgccg tcctgctcga caacaccttc ggcgaccggc ctgcgttccg ctttcggacg
      121 tcgccgcagg ctgccctcga ccgagcgcca cgtcacgtcg ctcgcgtatg acaccgtgcg
      181 cgcagcttca ctaatggcac gcagcaactc gtcggcgtcc gccatcccaa gacgagccgc
      241 gattgcctcc tgttcctgcg caaccatgcg ggtcgttggt ctgcctgcga cttcctgcaa
      301 ggtgtcacgg acgtcgatca gccggcgata tgccgcccgg accggcggtc ccgggccggc
      361 ggcaacccac gccgcggcaa tcgcttcgag tgcgtgcacg tcacgaagcc cgccacgtgc
      421 ctccttcaga tcgggttcaa gaaggaacgc cacttcgccg agtcgttcgg cccgatcgcg
      481 caccatctcg tggagttctg gtaggcgctt cgatgcgcgg gagcgccatt ggccgaatgc
      541 gtgctcgcga aggcgcgcgg tgagatcggg cactccggcg atgtgccgcg cgtgcagcat
      601 gccgagtgcg gccttcatgt cggcgtttgc caccgacacc gcgtccgcga ccgtgcgtac
      661 cgaatggtcg agctgccacc cggcgtccca gatcggatac cagacctgat cggcgatctg
      721 cacgacgtcc gaccggccgt cgtgcagcat catgacgtcg aggtcgctgc caggtccgag
      781 ttcacctcgc ccgtaaccac cgaccgcgac cagtgcaacg ccgtcagctc cgccgagcag
      841 ttcgcgcaac cacgcgtccg cctgcgccgt tcgttccgcg cgcaacgcag ccaggtcatg
      901 caccggctca ttcacagtcg cgccttcgac ggacacagcg gcgccggata ccgccacgcc
      961 ggaatcgtcg gtcgcagtcg aggcggcgtc atgagtgcgc gccacctctg ccccatccag
     1021 cgatgtcatg ggcttcagag agcgctgttt ccgcgctcgc cggtgcgaac gcgcaccacg
     1081 ttctcgacag gggttgacca caccttgccg tcaccgatct tgccggtttg cgccgacttc
     1141 acgatgacgt cgatgacatc gtcggcgtcg ccttcgtcga caagcacttc gatccggacc
     1201 ttgggcacga ggtcaacctc gtactcggcg ccacggtaga cctcggtgtg cccacgctgc
     1261 cggccgtagc cattcgcctc gctgactgtc atgccagtga cgccgaaggt ctcgagcgcc
     1321 gatttaacat cgtcgagctt gaacggcttg acgatcgcgg tgatcaatct catgaattca
     1381 ccttctcagt agcgccggca tggaccgtct caggggtgac tttgcccggg agaaagctcg
     1441 ccgagagtga tttccgaaat gggactccgt tgtttcacgg cagtgaaatc attacgtctc
     1501 gcgttacgca cgtgtaacgc ttcaagaacc cagaagtgcg tcgacgaacg cctcgggctc
     1561 aaacggcgcg agatcgtcag cgccttcacc cagaccgacc agcttgaccg ggacgccgag
     1621 ctcgcgctgt acggagatga cgatgccacc cttggcggtg ccgtcaagct tcgtcaggac
     1681 gacgccagtg atgtcgacga cctcggcaaa gactcgtgcc tgcacgaggc cgttctgccc
     1741 cgtggtcgcg tcgagcacca gcagcacttc gtcaaccggg gtctgcttct cgatcacccg
     1801 cttgaccttg ccgagctcgt ccatcaggcc tgctttggtg tgcagccgac cggcagtgtc
     1861 gacgagcacc gtgtcgaccc cgagttcgat gccggttttc actgcgtcga acgcgaccgc
     1921 tgcagggtca ccgccctcgg ggccacgaac ggtctgggcg cctactcggc gtccccacgt
     1981 ttcgagctgg tcagctgccg cggctctgaa ggtgtcggcg gcaccgagca ccacggttcg
     2041 tccttccgca acgagcagcc gggcgatctt gccgcacgtc gtcgtcttgc cggtgccatt
     2101 gaccccgacg acaagcacga tcgctgggcc ctcaccggcc gggcgctggg tcgccaacga
     2161 acggtcgagt tggggatcga cgagctcgag gagcccttcg cgaaggaatg cgcgtgcgtc
     2221 tgccgcggat ctggtgccga gcaccttcgc cttggttcgc agggaatcga cgagctcgtt
     2281 gcttgcactg acgccgacgt cggccgtgag cagcagatcc tcgacctcct gccacgtctc
     2341 gtcgtcgagc gtgtcacgcg acaacaacgc gagcagccct cgaccaaggg tcgattcgga
     2401 ccgtgcgaga cgggcccgca acctgaccag ccggccggct gccggttcgg gcgtctcggt
     2461 gaggtcaggc tcgagaacgg cctcggtgtc gacagcaggc tccgcggccg ctgagacact
     2521 cgcggcgttc ggtgctggtc gcagtggcgg cgcagactgg cgggg
//
